Cucsa.200370 (gene) Cucumber (Gy14) v1 | Cucurbit Genomics Database (CuGenDB)

Overview


Name	Cucsa.200370
Type	gene
Organism	Cucumis sativus (Cucumber (Gy14) v1)
Description	Pheophorbide a oxygenase
Location	scaffold01365 : 861741 .. 864907 (-)

Genome Viewer

Gene Expression

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDS

Hold the cursor over a type above to highlight its positions in the sequence below.

CCTGAGTTCTCATCAGTTACGATTCAGCGGGATTTATTTTATGGTTATGATACACTAATGGAGAATGTTCTCCATCAGATCCTTCCCACATAAATTTTGCTCATCACTAGGCAAGGGAGGGCCAACGTGAAAAACCTTTTCAACTAAACTTGAAATTTGCACCAGTGGTTTAGTTATTGATTGAATCATTATGGTCGTTTGTGTTTTTCTTTTCTTTCTGTTTCTGATGAATGCTAAGTGAAAGAAATGTGTCATATATAAAGAACTAAAAAACTTCAATGTTACCTGCTGGGTGCTGTTAAATATTTACTATGATTTAGAAGAGATTATGTTTCAGGAAACAAGAGCATTTGATAGGAACAGTCCTGCTGATATGGTAGTACTGTACTTCGTGTATATTAGTAAACTTGGTCTACAAAATCACATTTGTTTTTTGTTGAACATCACAATGTCTTGCTTCTGAAAGGAACATTTTCACAGGTTACAGGGAGGCAGGACCAAGCAAAACCTTTACCTTTCAAGGTGGATTCTAATGGTTCCTGGGGATTTTCTGGAGCAAATAAGGGTAACCCAAGGATTAGTGCCAAGTTTATTTCTCCTTGTTACTATATCAACAAGTAAAGATTTCACTCTTTTTCTCCTGTATTCACATTTGAACAATGCATAGCTTAAGATTGTTGGAACCATCCCTTACATATCTGATATTATACATCAATAGATATGCTTATGCATTTATGATGATTTGGTACCTCATGCATTAAGCGATAAATTTTTGGTAGCTTAGTATACTGGCTGATAGAGTGATAGTTTATTGACAGTGGGCTATTTCTCTCAATTCTATTCCAAATTTGGCCAGTCTTCTCTGGCATCTCTGTCTTCCTTATAAACTTCTTACTACTAACAGCTTAACTAACTCGCTTTTCCTAACCATATGCATAAAAAAGTTCTTTTCAATGATTATTCTACCTCCTTGATCCTACGTATGACCAATTGGACATGGGCATAAGCTTCATTCACTATCAAGTGTCAGTTTGCGTTATACATGTAAACAGATTGTCCAAGGATTCTTGAATCCAAAGCTGCAAATTTGAAGCAAATTATTTAATGTGTATTTGAACATTGTATGACCAACTATGAGTTTATGCTCAAATGACAAAAAGTTTCGAGCCTTACTAATTAAGGGATCAATTAGGATATAAATAACACATACACATACCATGATGACCATCCGGAATTTGGCAATTTTTTTTAAAAAGAAAAAAGTTTCATTTCTCACTCTTTCTTTTTGGATGCATTCCTGGATACAAATTTTAATAATGTTAGGCTGAGCAGGTAAGTATTATTCTTAAGAAATGTTTAATATTTGAAGTCAAGTATTATTTAATAATGTAGGGAGGGAAACGATAGTTAATAACAAGTGACCAGAGGAACCCAAGCACGTGAACCTTGAGACTTGAGTGTATTAGACCTGTTTAACGTTTCAGAATTTTTATAATTTTTCCACCATGAGAAAGACAGTATGTGCCATTTGAAATTATAGCTCTAGGAAATGGAAGGCAAAAAGAAATTAGAGAACTTTGATAGGAAAATGTTAGGAAAATTTAAAGTGCATTTGCTGTGCATCTTTGATCACAGGAATGTGTCATCAGTTATGAAGGTGAGGATATTGAGATCACGGTAAGTGAACAATTGCTTCAAGTTTGGTGAAATGAGCATGGGGTAGGAAATGAATAATGGAGTAGTCTTGAATGAGTATTCCAAGGAGGAGTACACCAGATTTTTTAATTGCCAAAATAGGTTTTAGGAATTTATATCCTTATTCCATTTTATGCATTCAAGAAGGAAGGCAAAACAGGCTGTGGTGTAAGGAAGGGAGAAATGAATAGTAAGAACTAACTAGAGGTTCTTTAGTTTTGTGAAATTACGAGACATCTCAAAACTTGACTCTTGGAGCTTTTCTCAAAAAAAAAAAAAAAAAAGTTACAATACAAGTCCTCTAGCATAAAATTAGCAACACCCTACTGAATTTATTGGCAACTGTTTCAACATTCTATTTCCTTTTGTTTAAGTGCTAAATATTATGAATCCCTTATACACAAATTCCTATAGGTTTTAACTTTATTGTAAAGTTTTCTTCGGAGTGGAGATTGACACAAAGCTTCCTTTACTTGGCGATAAAAAATGGGTAATGTGGATTTCCTCCTTCAATGTGCTAGTGGGTCCTGGAAAAATTCGTTCTATTGTATGTACTGCTCGAAACTTCTTCCAGTTTTCAATGGCCGGTCCTGTTTGATGGCAGGCAAGCAGTGCGGTGTTAATTAGATCTGTTTCTTATTTTTATGATATACTATCATCTCCACCGTTATTGGTCATTCAACATGCATTATATGTGATATTACAGTGTTTGACTTCCTAAGTTTGCTTTTTCTATTAGGTAGTTCCTCGATGGCACAAGCATTGGACTTGGAATAAGGTATATGATGGAGACATGATTGTTCTTTAGGGTCAAGAGAAGATATTTTTATCAATGGAAGGTTCGACAAATGTAAAGAATACACTAAAATAACGTTCACACCATAGCATTCAGAAATTGGCTGAATCGGCATGGAAAGGGCCAAAACCGAATAGTTCAGAGCTAGCAGCCACTAGCGCCTGTCATCAACAGTTCTATCTAGAACTCAGGTACTGAAAATTACCAGCATCTTGATTGTTTTTATAAAACTAAATGCTAAAAAGCATGAACACTTCAATTTAACTAGGAATATTCGTGTGCAATTTGTCCAATTTCTACCATTATTGTAAGTATGTAAGTCCAATTTTAACGATCCCAAGTGTAAGGACCTAATTTTTACTATTAAAAATAAGTGTGGTTGATGGGACTATTCTTTGGGTTGATTTTTGTCTTCTATTATCATCTCCTCTACAGACTACAGATGCTGGACAGATTTGAGCAACACACTCCATTGTTCATCATGTAAAGGAGCTTATAAAACATTTCAAACAGTGCAAAATTGCAAAAGCTCCTAATTGGTGCAGCTGTAGTTTTTGCGGCCAACGCCGGCATCTCTTCTGCTTTACAGATATGTATTCTTCTCACTAGTCTTGCACTCACAAGCACCGGCTTGGCTTACGCTCTGTTTGAACTTAAAAGTGAATTGTGTTTTTGA

mRNA sequence

CCTGAGTTCTCATCAGTTACGATTCAGCGGGATTTATTTTATGGTTATGATACACTAATGGAGAATGTTACAGGGAGGCAGGACCAAGCAAAACCTTTACCTTTCAAGGTGGATTCTAATGGTTCCTGGGGATTTTCTGGAGCAAATAAGGGTAACCCAAGGATTAGTGCCAAGTTTATTTCTCCTTTTTTCTTCGGAGTGGAGATTGACACAAAGCTTCCTTTACTTGGCGATAAAAAATGGGTAATGTGGATTTCCTCCTTCAATGTGCTAGTGGGTCCTGGAAAAATTCGTTCTATTGTATGTACTGCTCGAAACTTCTTCCAGTTTTCAATGGCCGGTCCTGTAGTTCCTCGATGGCACAAGCATTGGACTTGGAATAAGGTATATGATGGAGACATGATTCTCCTAATTGGTGCAGCTGTAGTTTTTGCGGCCAACGCCGGCATCTCTTCTGCTTTACAGATATGTATTCTTCTCACTAGTCTTGCACTCACAAGCACCGGCTTGGCTTACGCTCTGTTTGAACTTAAAAGTGAATTGTGTTTTTGA

Coding sequence (CDS)

CCTGAGTTCTCATCAGTTACGATTCAGCGGGATTTATTTTATGGTTATGATACACTAATGGAGAATGTTACAGGGAGGCAGGACCAAGCAAAACCTTTACCTTTCAAGGTGGATTCTAATGGTTCCTGGGGATTTTCTGGAGCAAATAAGGGTAACCCAAGGATTAGTGCCAAGTTTATTTCTCCTTTTTTCTTCGGAGTGGAGATTGACACAAAGCTTCCTTTACTTGGCGATAAAAAATGGGTAATGTGGATTTCCTCCTTCAATGTGCTAGTGGGTCCTGGAAAAATTCGTTCTATTGTATGTACTGCTCGAAACTTCTTCCAGTTTTCAATGGCCGGTCCTGTAGTTCCTCGATGGCACAAGCATTGGACTTGGAATAAGGTATATGATGGAGACATGATTCTCCTAATTGGTGCAGCTGTAGTTTTTGCGGCCAACGCCGGCATCTCTTCTGCTTTACAGATATGTATTCTTCTCACTAGTCTTGCACTCACAAGCACCGGCTTGGCTTACGCTCTGTTTGAACTTAAAAGTGAATTGTGTTTTTGA

Protein sequence

PEFSSVTIQRDLFYGYDTLMENVTGRQDQAKPLPFKVDSNGSWGFSGANKGNPRISAKFISPFFFGVEIDTKLPLLGDKKWVMWISSFNVLVGPGKIRSIVCTARNFFQFSMAGPVVPRWHKHWTWNKVYDGDMILLIGAAVVFAANAGISSALQICILLTSLALTSTGLAYALFELKSELCF*

Homology

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BLAST of Cucsa.200370 vs. Swiss-Prot
Match: PAO_ARATH (Pheophorbide a oxygenase, chloroplastic OS=Arabidopsis thaliana GN=PAO PE=1 SV=1)

HSP 1 Score: 211.1 bits (536), Expect = 1.0e-53
Identity = 107/187 (57.22%), Postives = 133/187 (71.12%), Query Frame = 1


Query: 1   PEFSSVTIQRDLFYGYDTLMENV-------------TGRQDQAKPLPFKVDSNGSWGFSG 60
           PEFS+VTIQRDLFYGYDTLMENV             TGR+D+AKPLPFKV+S+G WGF G
Sbjct: 225 PEFSTVTIQRDLFYGYDTLMENVSDPSHIDFAHHKVTGRRDRAKPLPFKVESSGPWGFQG 284

Query: 61  ANKGNPRISAKFISPFFF--GVEIDTKLPLLGDKKWVMWISSFNVLVGPGKIRSIVCTAR 120
           AN  +PRI+AKF++P +    +E+D KLP++G++KWV+WI SFN+ + PGK RSIVC+AR
Sbjct: 285 ANDDSPRITAKFVAPCYSMNKIELDAKLPIVGNQKWVIWICSFNIPMAPGKTRSIVCSAR 344

Query: 121 NFFQFSMAGP----VVPRWHKHWTWNKVYDGDMILLIGAAVVFAANAGISSALQICILLT 169
           NFFQFS+ GP    VVPRW++HWT N VYDGDMI+L G   VF A +  S    +    T
Sbjct: 345 NFFQFSVPGPAWWQVVPRWYEHWTSNLVYDGDMIVLQGQEKVFLAKSMESPDYDVNKQYT 404

HSP 2 Score: 40.8 bits (94), Expect = 1.9e-02
Identity = 21/47 (44.68%), Postives = 29/47 (61.70%), Query Frame = 1


Query: 137 LIGAAVVFAANAGISSALQICILLTSLALTSTGLAYALFELKSELCF 184
           L+GA V +AA AG+ S +QI ++L  L+L S   AYAL E +    F
Sbjct: 482 LVGATVFWAATAGVPSDVQIRLVLAGLSLISAASAYALHEQEKNFVF 528

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BLAST of Cucsa.200370 vs. TrEMBL
Match: C6L809_CUCSA (Putative pheide a oxygenase (Fragment) OS=Cucumis sativus GN=CsPAO PE=2 SV=1)

HSP 1 Score: 235.0 bits (598), Expect = 7.5e-59
Identity = 116/168 (69.05%), Postives = 134/168 (79.76%), Query Frame = 1


Query: 1   PEFSSVTIQRDLFYGYDTLMENV-------------TGRQDQAKPLPFKVDSNGSWGFSG 60
           PEFSSVTIQRDLFYGYDTLMENV             TGR+D+AKPLPFK+D+NG WGFSG
Sbjct: 10  PEFSSVTIQRDLFYGYDTLMENVSDPSHIDFAHHKVTGRRDRAKPLPFKLDTNGPWGFSG 69

Query: 61  ANKGNPRISAKFISPFFF--GVEIDTKLPLLGDKKWVMWISSFNVLVGPGKIRSIVCTAR 120
           ANKGNPRISA+F++P ++   VEID+KLPLLGD+KWV+WI SFNV +GPGK RSIVC+AR
Sbjct: 70  ANKGNPRISAEFVAPCYYINKVEIDSKLPLLGDQKWVIWICSFNVPMGPGKTRSIVCSAR 129

Query: 121 NFFQFSMAGP----VVPRWHKHWTWNKVYDGDMILLIGAAVVFAANAG 150
           NFFQFSM GP    VVPRWH+HWT NKVYDGDMI+L G   +F +  G
Sbjct: 130 NFFQFSMPGPAWWQVVPRWHEHWTSNKVYDGDMIVLQGQEKIFLSMEG 177

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BLAST of Cucsa.200370 vs. TrEMBL
Match: A0A0A0LSG3_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_1G132160 PE=4 SV=1)

HSP 1 Score: 235.0 bits (598), Expect = 7.5e-59
Identity = 116/168 (69.05%), Postives = 134/168 (79.76%), Query Frame = 1


Query: 1   PEFSSVTIQRDLFYGYDTLMENV-------------TGRQDQAKPLPFKVDSNGSWGFSG 60
           PEFSSVTIQRDLFYGYDTLMENV             TGR+D+AKPLPFK+D+NG WGFSG
Sbjct: 244 PEFSSVTIQRDLFYGYDTLMENVSDPSHIDFAHHKVTGRRDRAKPLPFKLDTNGPWGFSG 303

Query: 61  ANKGNPRISAKFISPFFF--GVEIDTKLPLLGDKKWVMWISSFNVLVGPGKIRSIVCTAR 120
           ANKGNPRISA+F++P ++   VEID+KLPLLGD+KWV+WI SFNV +GPGK RSIVC+AR
Sbjct: 304 ANKGNPRISAEFVAPCYYINKVEIDSKLPLLGDQKWVIWICSFNVPMGPGKTRSIVCSAR 363

Query: 121 NFFQFSMAGP----VVPRWHKHWTWNKVYDGDMILLIGAAVVFAANAG 150
           NFFQFSM GP    VVPRWH+HWT NKVYDGDMI+L G   +F +  G
Sbjct: 364 NFFQFSMPGPAWWQVVPRWHEHWTSNKVYDGDMIVLQGQEKIFLSMEG 411

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BLAST of Cucsa.200370 vs. TrEMBL
Match: A0A0A0LSG3_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_1G132160 PE=4 SV=1)

HSP 1 Score: 36.6 bits (83), Expect = 3.9e+01
Identity = 19/28 (67.86%), Postives = 20/28 (71.43%), Query Frame = 1


Query: 156 ICILLTSLALTSTGLAYALFELKSELCF 184
           I ILL SLAL S GLAYALFEL+    F
Sbjct: 483 IRILLASLALASAGLAYALFELQKNFVF 510

HSP 2 Score: 226.1 bits (575), Expect = 3.5e-56
Identity = 110/167 (65.87%), Postives = 133/167 (79.64%), Query Frame = 1


Query: 1   PEFSSVTIQRDLFYGYDTLMENV-------------TGRQDQAKPLPFKVDSNGSWGFSG 60
           PEFSSVTIQRDLFYGYDTLMENV             TGR+D+AKPLPFKV+++G WGF+G
Sbjct: 211 PEFSSVTIQRDLFYGYDTLMENVSDPSHIDFAHHKVTGRRDRAKPLPFKVEASGPWGFAG 270

Query: 61  ANKGNPRISAKFISPFFF--GVEIDTKLPLLGDKKWVMWISSFNVLVGPGKIRSIVCTAR 120
           AN+GNPRI+A+FI+P ++   +EIDTKLPL+GD+KW++WI SFNV + PGK RSIVC+AR
Sbjct: 271 ANEGNPRITAEFIAPCYYLNKIEIDTKLPLVGDQKWIIWICSFNVPMAPGKTRSIVCSAR 330

Query: 121 NFFQFSMAGP----VVPRWHKHWTWNKVYDGDMILLIGAAVVFAANA 149
           NFFQFSM GP    VVPRWH+HWT NKVYDGDMI+L G   VF + +
Sbjct: 331 NFFQFSMPGPAWWQVVPRWHEHWTSNKVYDGDMIVLQGQEKVFLSKS 377

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BLAST of Cucsa.200370 vs. TrEMBL
Match: M5WRF3_PRUPE (Uncharacterized protein (Fragment) OS=Prunus persica GN=PRUPE_ppa019738mg PE=4 SV=1)

HSP 1 Score: 51.6 bits (122), Expect = 1.2e-03
Identity = 31/59 (52.54%), Postives = 34/59 (57.63%), Query Frame = 1


Query: 125 TWNKVYDGDMILLIGAAVVFAANAGISSALQICILLTSLALTSTGLAYALFELKSELCF 184
           TW K       LLIGA VVF A AGI S LQ+ I+L   AL S  LAYAL EL+    F
Sbjct: 461 TWQK-------LLIGATVVFCATAGIPSDLQLRIILAGFALLSAALAYALRELEKNFVF 512

HSP 2 Score: 225.3 bits (573), Expect = 6.0e-56
Identity = 114/187 (60.96%), Postives = 140/187 (74.87%), Query Frame = 1


Query: 1   PEFSSVTIQRDLFYGYDTLMENV-------------TGRQDQAKPLPFKVDSNGSWGFSG 60
           PEFS+VTIQRDLFYGYDTLMENV             TGR+D+AKPLPFK+DS+G WGF+G
Sbjct: 221 PEFSTVTIQRDLFYGYDTLMENVSDPSHIDFAHHKVTGRRDRAKPLPFKMDSSGHWGFAG 280

Query: 61  ANKGNPRISAKFISPFFF--GVEIDTKLPLLGDKKWVMWISSFNVLVGPGKIRSIVCTAR 120
           AN GNPRISA+FI+P ++   +EIDTKLP++GD+KWV+WI SFNV + PGK RSIVC+AR
Sbjct: 281 ANDGNPRISAEFIAPCYYINKIEIDTKLPIVGDQKWVIWICSFNVPMAPGKTRSIVCSAR 340

Query: 121 NFFQFSMAGP----VVPRWHKHWTWNKVYDGDMILLIGAAVVFAANAGISSALQICILLT 169
           NFFQ +M GP    VVPRWH+HWT NKVYDGDMI+L G   VF + + + S+  +    T
Sbjct: 341 NFFQLTMPGPAWWQVVPRWHEHWTSNKVYDGDMIVLQGQEKVFLSKS-MESSTDVNKEYT 400

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BLAST of Cucsa.200370 vs. TrEMBL
Match: V4SFU9_9ROSI (Uncharacterized protein OS=Citrus clementina GN=CICLE_v10028147mg PE=4 SV=1)

HSP 1 Score: 224.9 bits (572), Expect = 7.8e-56
Identity = 108/167 (64.67%), Postives = 132/167 (79.04%), Query Frame = 1


Query: 1   PEFSSVTIQRDLFYGYDTLMENV-------------TGRQDQAKPLPFKVDSNGSWGFSG 60
           PEFSSVTIQRDLFYGYDTLMENV             TGR+D+AKPLPFK++S+G WGF+G
Sbjct: 231 PEFSSVTIQRDLFYGYDTLMENVSDPSHIDFAHHKVTGRRDRAKPLPFKLESSGHWGFAG 290

Query: 61  ANKGNPRISAKFISPFFF--GVEIDTKLPLLGDKKWVMWISSFNVLVGPGKIRSIVCTAR 120
           AN GNPRISAKF++P ++   +EIDTKLP++GD+KW++WI SFNV + PGK RSIVC+AR
Sbjct: 291 ANDGNPRISAKFVAPCYYMNKIEIDTKLPVVGDQKWIIWICSFNVPMAPGKTRSIVCSAR 350

Query: 121 NFFQFSMAGP----VVPRWHKHWTWNKVYDGDMILLIGAAVVFAANA 149
           NFFQF+M GP    VVPRWH+HWT NKVYDGDMI+L G   +F + +
Sbjct: 351 NFFQFTMPGPAWWQVVPRWHEHWTSNKVYDGDMIVLQGQEKIFLSKS 397

[Close]

BLAST of Cucsa.200370 vs. TAIR10
Match: AT3G44880.1 (AT3G44880.1 Pheophorbide a oxygenase family protein with Rieske [2Fe-2S] domain)

HSP 1 Score: 211.1 bits (536), Expect = 5.9e-55
Identity = 107/187 (57.22%), Postives = 133/187 (71.12%), Query Frame = 1


Query: 1   PEFSSVTIQRDLFYGYDTLMENV-------------TGRQDQAKPLPFKVDSNGSWGFSG 60
           PEFS+VTIQRDLFYGYDTLMENV             TGR+D+AKPLPFKV+S+G WGF G
Sbjct: 225 PEFSTVTIQRDLFYGYDTLMENVSDPSHIDFAHHKVTGRRDRAKPLPFKVESSGPWGFQG 284

Query: 61  ANKGNPRISAKFISPFFF--GVEIDTKLPLLGDKKWVMWISSFNVLVGPGKIRSIVCTAR 120
           AN  +PRI+AKF++P +    +E+D KLP++G++KWV+WI SFN+ + PGK RSIVC+AR
Sbjct: 285 ANDDSPRITAKFVAPCYSMNKIELDAKLPIVGNQKWVIWICSFNIPMAPGKTRSIVCSAR 344

Query: 121 NFFQFSMAGP----VVPRWHKHWTWNKVYDGDMILLIGAAVVFAANAGISSALQICILLT 169
           NFFQFS+ GP    VVPRW++HWT N VYDGDMI+L G   VF A +  S    +    T
Sbjct: 345 NFFQFSVPGPAWWQVVPRWYEHWTSNLVYDGDMIVLQGQEKVFLAKSMESPDYDVNKQYT 404

HSP 2 Score: 40.8 bits (94), Expect = 1.1e-03
Identity = 21/47 (44.68%), Postives = 29/47 (61.70%), Query Frame = 1


Query: 137 LIGAAVVFAANAGISSALQICILLTSLALTSTGLAYALFELKSELCF 184
           L+GA V +AA AG+ S +QI ++L  L+L S   AYAL E +    F
Sbjct: 482 LVGATVFWAATAGVPSDVQIRLVLAGLSLISAASAYALHEQEKNFVF 528

[Close]

BLAST of Cucsa.200370 vs. NCBI nr
Match: gi|659071896|ref|XP_008462467.1| (PREDICTED: pheophorbide a oxygenase, chloroplastic [Cucumis melo])

HSP 1 Score: 239.6 bits (610), Expect = 4.4e-60
Identity = 120/168 (71.43%), Postives = 134/168 (79.76%), Query Frame = 1


Query: 1   PEFSSVTIQRDLFYGYDTLMENV-------------TGRQDQAKPLPFKVDSNGSWGFSG 60
           PEFSSVTIQRDLFYGYDTLMENV             TGR+D+AKPLPFK+DSNG WGFSG
Sbjct: 237 PEFSSVTIQRDLFYGYDTLMENVSDPSHIDFAHHKVTGRRDRAKPLPFKLDSNGPWGFSG 296

Query: 61  ANKGNPRISAKFISPFFF--GVEIDTKLPLLGDKKWVMWISSFNVLVGPGKIRSIVCTAR 120
           ANKGNPRISAKFI+P ++   VEIDTKLPLLGD+KWV+WI SFNV +GPGK RSIVC+AR
Sbjct: 297 ANKGNPRISAKFIAPCYYINKVEIDTKLPLLGDQKWVIWICSFNVPMGPGKTRSIVCSAR 356

Query: 121 NFFQFSMAGP----VVPRWHKHWTWNKVYDGDMILLIGAAVVFAANAG 150
           NFFQFSM GP    VVPRWH+HWT NKVYDGDMI+L G   +F +  G
Sbjct: 357 NFFQFSMPGPAWWQVVPRWHEHWTSNKVYDGDMIVLQGQEKIFLSMEG 404

[Close]

BLAST of Cucsa.200370 vs. NCBI nr
Match: gi|659071896|ref|XP_008462467.1| (PREDICTED: pheophorbide a oxygenase, chloroplastic [Cucumis melo])

HSP 1 Score: 62.4 bits (150), Expect = 9.6e-07
Identity = 33/48 (68.75%), Postives = 37/48 (77.08%), Query Frame = 1


Query: 136 LLIGAAVVFAANAGISSALQICILLTSLALTSTGLAYALFELKSELCF 184
           LLIGA VVF+A AGI S+LQI +LL SLAL S GLAYALFEL+    F
Sbjct: 489 LLIGATVVFSATAGIPSSLQIRVLLASLALASAGLAYALFELQKNFVF 536

HSP 2 Score: 235.0 bits (598), Expect = 1.1e-58
Identity = 116/168 (69.05%), Postives = 134/168 (79.76%), Query Frame = 1


Query: 1   PEFSSVTIQRDLFYGYDTLMENV-------------TGRQDQAKPLPFKVDSNGSWGFSG 60
           PEFSSVTIQRDLFYGYDTLMENV             TGR+D+AKPLPFK+D+NG WGFSG
Sbjct: 238 PEFSSVTIQRDLFYGYDTLMENVSDPSHIDFAHHKVTGRRDRAKPLPFKLDTNGPWGFSG 297

Query: 61  ANKGNPRISAKFISPFFF--GVEIDTKLPLLGDKKWVMWISSFNVLVGPGKIRSIVCTAR 120
           ANKGNPRISA+F++P ++   VEID+KLPLLGD+KWV+WI SFNV +GPGK RSIVC+AR
Sbjct: 298 ANKGNPRISAEFVAPCYYINKVEIDSKLPLLGDQKWVIWICSFNVPMGPGKTRSIVCSAR 357

Query: 121 NFFQFSMAGP----VVPRWHKHWTWNKVYDGDMILLIGAAVVFAANAG 150
           NFFQFSM GP    VVPRWH+HWT NKVYDGDMI+L G   +F +  G
Sbjct: 358 NFFQFSMPGPAWWQVVPRWHEHWTSNKVYDGDMIVLQGQEKIFLSMEG 405

[Close]

BLAST of Cucsa.200370 vs. NCBI nr
Match: gi|778659272|ref|XP_011654100.1| (PREDICTED: LOW QUALITY PROTEIN: pheophorbide a oxygenase, chloroplastic [Cucumis sativus])

HSP 1 Score: 65.1 bits (157), Expect = 1.5e-07
Identity = 36/48 (75.00%), Postives = 37/48 (77.08%), Query Frame = 1


Query: 136 LLIGAAVVFAANAGISSALQICILLTSLALTSTGLAYALFELKSELCF 184
           LLIGA VVFAA AGI SALQI ILL SLAL S GLAYALFEL+    F
Sbjct: 490 LLIGATVVFAATAGIPSALQIRILLASLALASAGLAYALFELQKNFVF 537

HSP 2 Score: 235.0 bits (598), Expect = 1.1e-58
Identity = 116/168 (69.05%), Postives = 134/168 (79.76%), Query Frame = 1


Query: 1   PEFSSVTIQRDLFYGYDTLMENV-------------TGRQDQAKPLPFKVDSNGSWGFSG 60
           PEFSSVTIQRDLFYGYDTLMENV             TGR+D+AKPLPFK+D+NG WGFSG
Sbjct: 10  PEFSSVTIQRDLFYGYDTLMENVSDPSHIDFAHHKVTGRRDRAKPLPFKLDTNGPWGFSG 69

Query: 61  ANKGNPRISAKFISPFFF--GVEIDTKLPLLGDKKWVMWISSFNVLVGPGKIRSIVCTAR 120
           ANKGNPRISA+F++P ++   VEID+KLPLLGD+KWV+WI SFNV +GPGK RSIVC+AR
Sbjct: 70  ANKGNPRISAEFVAPCYYINKVEIDSKLPLLGDQKWVIWICSFNVPMGPGKTRSIVCSAR 129

Query: 121 NFFQFSMAGP----VVPRWHKHWTWNKVYDGDMILLIGAAVVFAANAG 150
           NFFQFSM GP    VVPRWH+HWT NKVYDGDMI+L G   +F +  G
Sbjct: 130 NFFQFSMPGPAWWQVVPRWHEHWTSNKVYDGDMIVLQGQEKIFLSMEG 177

[Close]

BLAST of Cucsa.200370 vs. NCBI nr
Match: gi|700209759|gb|KGN64855.1| (hypothetical protein Csa_1G132160 [Cucumis sativus])

HSP 1 Score: 235.0 bits (598), Expect = 1.1e-58
Identity = 116/168 (69.05%), Postives = 134/168 (79.76%), Query Frame = 1


Query: 1   PEFSSVTIQRDLFYGYDTLMENV-------------TGRQDQAKPLPFKVDSNGSWGFSG 60
           PEFSSVTIQRDLFYGYDTLMENV             TGR+D+AKPLPFK+D+NG WGFSG
Sbjct: 244 PEFSSVTIQRDLFYGYDTLMENVSDPSHIDFAHHKVTGRRDRAKPLPFKLDTNGPWGFSG 303

Query: 61  ANKGNPRISAKFISPFFF--GVEIDTKLPLLGDKKWVMWISSFNVLVGPGKIRSIVCTAR 120
           ANKGNPRISA+F++P ++   VEID+KLPLLGD+KWV+WI SFNV +GPGK RSIVC+AR
Sbjct: 304 ANKGNPRISAEFVAPCYYINKVEIDSKLPLLGDQKWVIWICSFNVPMGPGKTRSIVCSAR 363

Query: 121 NFFQFSMAGP----VVPRWHKHWTWNKVYDGDMILLIGAAVVFAANAG 150
           NFFQFSM GP    VVPRWH+HWT NKVYDGDMI+L G   +F +  G
Sbjct: 364 NFFQFSMPGPAWWQVVPRWHEHWTSNKVYDGDMIVLQGQEKIFLSMEG 411

[Close]

BLAST of Cucsa.200370 vs. NCBI nr
Match: gi|700209759|gb|KGN64855.1| (hypothetical protein Csa_1G132160 [Cucumis sativus])

HSP 1 Score: 36.6 bits (83), Expect = 5.6e+01
Identity = 19/28 (67.86%), Postives = 20/28 (71.43%), Query Frame = 1


Query: 156 ICILLTSLALTSTGLAYALFELKSELCF 184
           I ILL SLAL S GLAYALFEL+    F
Sbjct: 483 IRILLASLALASAGLAYALFELQKNFVF 510

HSP 2 Score: 227.6 bits (579), Expect = 1.7e-56
Identity = 111/167 (66.47%), Postives = 133/167 (79.64%), Query Frame = 1


Query: 1   PEFSSVTIQRDLFYGYDTLMENV-------------TGRQDQAKPLPFKVDSNGSWGFSG 60
           PEFSSVTIQRDLFYGYDTLMENV             TGR+D+AKPLPFKV+++G WGF+G
Sbjct: 238 PEFSSVTIQRDLFYGYDTLMENVSDPSHIDFAHHKVTGRRDRAKPLPFKVEASGPWGFAG 297

Query: 61  ANKGNPRISAKFISPFFF--GVEIDTKLPLLGDKKWVMWISSFNVLVGPGKIRSIVCTAR 120
           AN+GNPRI+A+FI+P ++   +EIDTKLPL+GDKKW++WI SFNV + PGK RSIVC+AR
Sbjct: 298 ANEGNPRITAEFIAPSYYLNKIEIDTKLPLVGDKKWIIWICSFNVPMAPGKTRSIVCSAR 357

Query: 121 NFFQFSMAGP----VVPRWHKHWTWNKVYDGDMILLIGAAVVFAANA 149
           NFFQFSM GP    VVPRWH+HWT NKVYDGDMI+L G   VF + +
Sbjct: 358 NFFQFSMPGPAWWQVVPRWHEHWTSNKVYDGDMIVLQGQEKVFLSKS 404

The following BLAST results are available for this feature:

BLAST of Cucsa.200370 vs. Swiss-Prot


Match Name	E-value	Identity	Description
PAO_ARATH	1.0e-53	57.22	Pheophorbide a oxygenase, chloroplastic OS=Arabidopsis thaliana GN=PAO PE=1 SV=1	[more]

BLAST of Cucsa.200370 vs. TrEMBL


Match Name	E-value	Identity	Description
C6L809_CUCSA	7.5e-59	69.05	Putative pheide a oxygenase (Fragment) OS=Cucumis sativus GN=CsPAO PE=2 SV=1	[more]
A0A0A0LSG3_CUCSA	7.5e-59	69.05	Uncharacterized protein OS=Cucumis sativus GN=Csa_1G132160 PE=4 SV=1	[more]
A0A0A0LSG3_CUCSA	3.9e+01	67.86	Uncharacterized protein OS=Cucumis sativus GN=Csa_1G132160 PE=4 SV=1	[more]
M5WRF3_PRUPE	1.2e-03	52.54	Uncharacterized protein (Fragment) OS=Prunus persica GN=PRUPE_ppa019738mg PE=4 S...	[more]
V4SFU9_9ROSI	7.8e-56	64.67	Uncharacterized protein OS=Citrus clementina GN=CICLE_v10028147mg PE=4 SV=1	[more]

BLAST of Cucsa.200370 vs. TAIR10


Match Name	E-value	Identity	Description
AT3G44880.1	5.9e-55	57.22	Pheophorbide a oxygenase family protein with Rieske [2Fe-2S] domain	[more]

BLAST of Cucsa.200370 vs. NCBI nr


Match Name	E-value	Identity	Description
gi\|659071896\|ref\|XP_008462467.1\|	4.4e-60	71.43	PREDICTED: pheophorbide a oxygenase, chloroplastic [Cucumis melo]	[more]
gi\|659071896\|ref\|XP_008462467.1\|	9.6e-07	68.75	PREDICTED: pheophorbide a oxygenase, chloroplastic [Cucumis melo]	[more]
gi\|778659272\|ref\|XP_011654100.1\|	1.5e-07	75.00	PREDICTED: LOW QUALITY PROTEIN: pheophorbide a oxygenase, chloroplastic [Cucumis...	[more]
gi\|700209759\|gb\|KGN64855.1\|	1.1e-58	69.05	hypothetical protein Csa_1G132160 [Cucumis sativus]	[more]
gi\|700209759\|gb\|KGN64855.1\|	5.6e+01	67.86	hypothetical protein Csa_1G132160 [Cucumis sativus]	[more]

Annotated Terms

The following terms have been associated with this gene:

Vocabulary: **INTERPRO**
Term	Definition
IPR013626	PaO

Vocabulary: **Molecular Function**
Term	Definition
GO:0010277	chlorophyllide a oxygenase [overall] activity

Vocabulary: **Biological Process**
Term	Definition
GO:0055114	oxidation-reduction process

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0055114	oxidation-reduction process
biological_process	GO:0048608	reproductive structure development
biological_process	GO:0044763	single-organism cellular process
cellular_component	GO:0044434	chloroplast part
cellular_component	GO:0005575	cellular_component
molecular_function	GO:0051537	2 iron, 2 sulfur cluster binding
molecular_function	GO:0010277	chlorophyllide a oxygenase [overall] activity
molecular_function	GO:0046872	metal ion binding

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Cucsa.200370.1	Cucsa.200370.1	mRNA

InterPro

Analysis Name: InterPro Annotations of cucumber (Gy14)
Date Performed: 2017-01-17


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR013626	Pheophorbide a oxygenase	PFAM	PF08417	PaO	coord: 77..140 score: 2.2
None	No IPR available	PANTHER	PTHR21266	IRON-SULFUR DOMAIN CONTAINING PROTEIN	coord: 1..144 score: 1.6
None	No IPR available	PANTHER	PTHR21266:SF24	PHEOPHORBIDE A OXYGENASE, CHLOROPLASTIC	coord: 1..144 score: 1.6

Synteny

The following gene(s) are orthologous to this gene:


Gene	Orthologue	Organism	Block
Cucsa.200370	Cla001857	Watermelon (97103) v1	cgywmB391
Cucsa.200370	MELO3C004867	Melon (DHL92) v3.5.1	cgymeB365
Cucsa.200370	ClCG01G013870	Watermelon (Charleston Gray)	cgywcgB371
Cucsa.200370	CmaCh13G002620	Cucurbita maxima (Rimu)	cgycmaB0594
Cucsa.200370	CmoCh13G002690	Cucurbita moschata (Rifu)	cgycmoB0594
Cucsa.200370	Lsi03G000580	Bottle gourd (USVL1VR-Ls)	cgylsiB343
Cucsa.200370	Cp4.1LG20g07340	Cucurbita pepo (Zucchini)	cgycpeB0565
Cucsa.200370	MELO3C004867.2	Melon (DHL92) v3.6.1	cgymedB361
Cucsa.200370	Cla97C01G012850	Watermelon (97103) v2	cgywmbB374
Cucsa.200370	Bhi08G000125	Wax gourd	cgywgoB422
Cucsa.200370	Carg23702	Silver-seed gourd	carcgyB0205

The following gene(s) are paralogous to this gene:

None

The following block(s) are covering this gene:


Gene	Organism	Block
Cucsa.200370	Cucumber (Chinese Long) v3	cgycucB322
Cucsa.200370	Cucurbita maxima (Rimu)	cgycmaB0593
Cucsa.200370	Cucurbita moschata (Rifu)	cgycmoB0593
Cucsa.200370	Wild cucumber (PI 183967)	cgycpiB318
Cucsa.200370	Cucumber (Chinese Long) v2	cgycuB305