Cucsa.198810 (gene) Cucumber (Gy14) v1

BLAST of Cucsa.198810 vs. Swiss-Prot
Match: EI2BD_MOUSE (Translation initiation factor eIF-2B subunit delta OS=Mus musculus GN=Eif2b4 PE=1 SV=2)

HSP 1 Score: 345.5 bits (885), Expect = 1.3e-93
Identity = 215/551 (39.02%), Postives = 313/551 (56.81%), Query Frame = 1

Query: 93  SYNPSETLLATSPMASPSSRTGDEELDMSEDSSNSVWHRRSGSGKIASSFPGVGIHVGAK 152
           S +  +T L+  P A+    T +E+L + ++       R+   G        +G  V A 
Sbjct: 12  SRSEMKTELSPRPGAAGRELTQEEKLQLRKEKKQQKKKRKEEKGADQE----IGSAVSAA 71

Query: 153 SLENSQAVPLQTLETNKPAGVGEKTGGPSTEVQNRQPPPNSKPLKAKTTKAERRALQEAQ 212
             ++    P++ L      G G + GG + E   + P   SK       +AERRA QEA+
Sbjct: 72  QRQD----PIRELP-----GPGSQLGGTAGE---KLPAGRSKA----ELRAERRAKQEAE 131

Query: 213 RAAKVSAKGEANKSGAAPGKAVKQPAAMKDSTAAPSVAPTDKRGGGDRTLDKERKKDAPP 272
           RA K + KGE  + G  P              A PS A       G+ T   +R  +  P
Sbjct: 132 RALKQARKGE--QGGVPP-------------QACPSTA-------GETTSGVKRVPEHTP 191

Query: 273 PRMQFDDKSRVEKAKKRAVFNQTEARNRVELFRHLPQYEQGTQLPDLVSKFFQLDSIHPA 332
                  +  + K  ++ V  + +  ++V LF HLPQY + + L   +S       IHPA
Sbjct: 192 ADDPTLLRRLLRKPDRQQVPTRKDYGSKVSLFSHLPQYSRQSSLTQYMS--IPSSVIHPA 251

Query: 333 IYKVGLQYMARDICGGNARCIAMLHAFQQAIKDYSTPPQKSLGRDLTAKIGSYVSFFNEC 392
           + ++GLQY    I G NARCIA+LHA QQ I+DY+TPP + L RDL  K+  Y+SF  +C
Sbjct: 252 MVRLGLQYSQGLISGSNARCIALLHALQQVIQDYTTPPSEELSRDLVNKLKPYISFLTQC 311

Query: 393 RPLSVSMGNAIRFLKNRIAKLPLNLPESEVKATLCSDINRFINEKIIIADEVIVRHAATK 452
           RP+S SM NAI+FL   +  +  +  E E K+ L   ++R++ EKI++A + I R A+TK
Sbjct: 312 RPMSASMCNAIKFLTKEVTGMSSSKREEEAKSELREALDRYVQEKIVLAAQAISRFASTK 371

Query: 453 IRDGDVLLTYGSSSAVEMLILYAHELGKEFRVVVVDSRPKQEGKALLRRLIARGLNCTYT 512
           I DGDV+L YG SS V  ++  A   G+ FRVVVVDSRP+ EG+ +L  L+  G+  +Y 
Sbjct: 372 ISDGDVILVYGCSSLVSRILQEARVEGRRFRVVVVDSRPRLEGRHMLHSLVRAGVPTSYL 431

Query: 513 HINAISYVMHEVTRVFLGAAAVLSNGTVYSRVGTAAVAMVANAFHVPVLICCEAYKFHER 572
            I A SYV+ EV++V LGA A+L+NG+V SRVGTA +A+VA A +VPVL+CCE YKF ER
Sbjct: 432 LIPAASYVLPEVSKVLLGAHALLANGSVMSRVGTAQLALVARAHNVPVLVCCETYKFCER 491

Query: 573 VQLDSICFNELGDPDTIS-KVPERKDINDLAAKEQLQLLNLMYDATPSDYISMIVTDYGM 632
           VQ D+   NEL DPD +  K  ++  + +  +   L+LLNL+YD TP + + +++T+ GM
Sbjct: 492 VQTDAFVSNELDDPDDLQCKRGDQVALANWQSHPSLRLLNLVYDVTPPELVDLVITELGM 518

Query: 633 IPPTSVPVIVR 643
           IP +SVPV++R
Sbjct: 552 IPCSSVPVVLR 518

BLAST of Cucsa.198810 vs. Swiss-Prot
Match: EI2BD_RAT (Translation initiation factor eIF-2B subunit delta OS=Rattus norvegicus GN=Eif2b4 PE=2 SV=1)

HSP 1 Score: 344.7 bits (883), Expect = 2.1e-93
Identity = 216/555 (38.92%), Postives = 313/555 (56.40%), Query Frame = 1

Query: 93  SYNPSETLLATSPMASPSSRTGDEELDMSEDSSNSVWHRRSGSGKIASSFPGVGIHVGAK 152
           S +  +T L+  P A+    T +E+L + ++       R+   G        +G  V A 
Sbjct: 12  SRSEMKTELSPRPGAAGRELTQEEKLQLRKEKKQQKKKRKEEKGADQE----IGSAVSAA 71

Query: 153 SLENSQAVPLQTLETNKPAGVGEKTGGPSTEVQNRQPPPNSKPLKAKTTKAERRALQEAQ 212
             ++    P++ L+     G G + GG + E   + P   SK       +AERRA QEA+
Sbjct: 72  QRQD----PVRELQ-----GTGSQLGGTTGE---KLPAGRSKA----ELRAERRAKQEAE 131

Query: 213 RAAKVSAKGEANKSGAAPGKAVKQPAAMKDSTAAPSVAPTDKRGGGDRTLDKERKKDAPP 272
           RA K + KGE  + G +P              A PS A       G+ T   +R     P
Sbjct: 132 RALKQARKGE--QGGPSP-------------QACPSTA-------GEATSGVKRV----P 191

Query: 273 PRMQFDDKSRVEKAKKRAVFNQTEAR----NRVELFRHLPQYEQGTQLPDLVSKFFQLDS 332
              Q DD + + +  ++    Q   R    ++V LF HLPQY + + L   +S       
Sbjct: 192 EHTQADDPTLLRRLLRKPDRQQVPTRKDYGSKVSLFSHLPQYSRQSSLTQYMS--IPSSV 251

Query: 333 IHPAIYKVGLQYMARDICGGNARCIAMLHAFQQAIKDYSTPPQKSLGRDLTAKIGSYVSF 392
           IHPA+ ++GLQY    + G NARCIA+LHA QQ I+DY+TPP + L RDL  K+  Y+SF
Sbjct: 252 IHPAMVRLGLQYSQGLVSGSNARCIALLHALQQVIQDYTTPPNEELSRDLVNKLKPYISF 311

Query: 393 FNECRPLSVSMGNAIRFLKNRIAKLPLNLPESEVKATLCSDINRFINEKIIIADEVIVRH 452
             +CRP+S SM NAI+F    +  +  +  E E K+ L   I+R++ EKI++A + I R 
Sbjct: 312 LTQCRPMSASMCNAIKFFNKEVTGMSSSKREEEAKSELKEAIDRYVQEKIVLASQAISRF 371

Query: 453 AATKIRDGDVLLTYGSSSAVEMLILYAHELGKEFRVVVVDSRPKQEGKALLRRLIARGLN 512
           A+ KI DGDV+L YG SS V  ++  A   G+ FRVVVVDSRP+ EG+ +L  L+  G+ 
Sbjct: 372 ASKKISDGDVILVYGCSSLVSRILQEAWVEGRRFRVVVVDSRPRLEGRHMLHCLVRAGVP 431

Query: 513 CTYTHINAISYVMHEVTRVFLGAAAVLSNGTVYSRVGTAAVAMVANAFHVPVLICCEAYK 572
            +Y  I A SYV+ EV++V LGA A+L+NG+V SRVGTA +A+VA A +VPVL+CCE YK
Sbjct: 432 TSYLLIPAASYVLPEVSKVLLGAHALLANGSVMSRVGTAQLALVARAHNVPVLVCCETYK 491

Query: 573 FHERVQLDSICFNELGDPDTIS-KVPERKDINDLAAKEQLQLLNLMYDATPSDYISMIVT 632
           F ERVQ D+   NEL DPD +  K  ++  + +      L+LLNL+YD TP + + +++T
Sbjct: 492 FCERVQTDAFVSNELDDPDDLQCKRGDQVTLANWQNNSSLRLLNLVYDVTPPELVDLVIT 518

Query: 633 DYGMIPPTSVPVIVR 643
           + GMIP +SVPV++R
Sbjct: 552 ELGMIPCSSVPVVLR 518

BLAST of Cucsa.198810 vs. Swiss-Prot
Match: EI2BD_DICDI (Translation initiation factor eIF-2B subunit delta OS=Dictyostelium discoideum GN=eif2b4 PE=3 SV=1)

HSP 1 Score: 342.8 bits (878), Expect = 8.2e-93
Identity = 225/647 (34.78%), Postives = 344/647 (53.17%), Query Frame = 1

Query: 11  NPKVRQVGFFTLDAPNSPDRSLSGPPNCLPSQQSLSPPTNSLSPVMIPPPRHLSDNLASR 70
           N   R  G    +  +  D    G P     Q+++   TN+ SPV IP            
Sbjct: 7   NSSYRDTGASLKELGDYLDSKQEGKPT---HQKTIGFDTNT-SPVSIP------------ 66

Query: 71  SAVLPVPAPANWRNSTDQIPVGSYNPSETLLATSPMASPSSRTGDEELDMSEDSSNSVWH 130
             ++  P  +N  ++  + P  SY+  +T    SP+ S S+          ++++N+  +
Sbjct: 67  -TIISPPLGSN-NSNYGKSPKSSYDNKQT----SPLLSASNNR--------KNNNNNNNN 126

Query: 131 RRSGSGKIASSFPGVGIHVGAKSLENSQAVPLQTLETNKPAGVGEKTGGPSTEVQNRQPP 190
             + S K +S      ++     + +S    L  L+  K   +G  +  P++   +   P
Sbjct: 127 NNATSPKDSSIIGKNNVNSDLSKVSSS----LNELKFEKQP-IGSTSSTPTSTPSST--P 186

Query: 191 PNSKPLKAKTTKAERRALQEAQRAAKVSAKGEANKSGAAPGKAVKQPAAMKDST-AAPSV 250
            +S P    +T       Q+ Q+  +   + +  K    P +  KQ A  +D        
Sbjct: 187 SSSTPSSTPSTPNTNSQQQQQQQQQQKKQQQQQPKQPQQPKQQSKQQATQQDKKDKEQQQ 246

Query: 251 APTDKRGGGDRTL--DKERKKDAPPPRMQFDDKSRVEKAKKRAVFNQTEARNRVELFRHL 310
              DK+      +   KE  KD      QFDD  +  K  K+ +    E+   V+LF HL
Sbjct: 247 QQQDKQDKESNEIKGSKEVAKDGQHGVKQFDDPKQRGKITKKKIIKVGESSRSVQLFNHL 306

Query: 311 PQYEQGTQLPDLVSKFFQLDS--IHPAIYKVGLQYMARDICGGNARCIAMLHAFQQAIKD 370
           PQY     +   VS     +   IHP I  +GL+Y    I G NAR IAM+ AF Q  KD
Sbjct: 307 PQYNSEFSMGVSVSTDEPNEKYPIHPDIISLGLKYAEFKIAGSNARAIAMMTAFIQIFKD 366

Query: 371 YSTPPQKSLGRDLTAKIGSYVSFFNECRPLSVSMGNAIRFLKNRIAKLPLNLPESEVKAT 430
           Y   P K   R+L + +   + F  +CRP+S+SMGN+I ++K++++ L  N+     +  
Sbjct: 367 YVAAPDKVYSRELDSLLKRNIQFLVDCRPISISMGNSINYVKHKLS-LTNNMSHEGARDY 426

Query: 431 LCSDINRFINEKIIIADEVIVRHAATKIRDGDVLLTYGSSSAVEMLILYAHELGKEFRVV 490
           L   IN FI E+I +AD  IV+H  +KI DGDV+LTY SS  VE++I  A +  K+FR++
Sbjct: 427 LIKSINEFI-ERIQMADNAIVKHGCSKINDGDVILTYASSHVVELIIQQAIQDKKKFRLI 486

Query: 491 VVDSRPKQEGKALLRRLIARGLNCTYTHINAISYVMHEVTRVFLGAAAVLSNGTVYSRVG 550
           +VDSRPK EG+ LL RL+  G+  TY  ++A+SY+M EVT+VF+GA +VLSNG + SR G
Sbjct: 487 IVDSRPKHEGRELLHRLVLHGVKITYIMLHAVSYIMKEVTKVFVGAYSVLSNGNLISRSG 546

Query: 551 TAAVAMVANAFHVPVLICCEAYKFHERVQLDSICFNELGDP-DTISKVPERKDINDL--- 610
           T+ VA +A  ++VP ++CCE YKF ERVQLDSICFN++G+P D +  + E++    L   
Sbjct: 547 TSLVASMAKFYNVPFIVCCETYKFTERVQLDSICFNQIGNPQDLVQNLGEKEGSKSLLEN 606

Query: 611 -AAKEQLQLLNLMYDATPSDYISMIVTDYGMIPPTSVPVIVREYRRE 648
             +   L+LLNLMYD TP + I M++T++GM+PPTS+PV++REYR+E
Sbjct: 607 WESYSTLKLLNLMYDLTPIELIDMVITEFGMLPPTSIPVVLREYRKE 614

BLAST of Cucsa.198810 vs. Swiss-Prot
Match: EI2BD_RABIT (Translation initiation factor eIF-2B subunit delta OS=Oryctolagus cuniculus GN=EIF2B4 PE=2 SV=2)

HSP 1 Score: 339.3 bits (869), Expect = 9.0e-92
Identity = 215/545 (39.45%), Postives = 306/545 (56.15%), Query Frame = 1

Query: 101 LATSPMASPSSRTGDEELDMSEDSSNSVWHRRSGSGKIASSFPGVGIHVGAKSLENSQAV 160
           L+  P A     T +E+L + ++       R+   G    +   V     A+    ++  
Sbjct: 20  LSARPGAGGKEMTQEEKLQLRKEKKQQKKKRKEEKGTDVDTASAVS---AAQCPGPAREA 79

Query: 161 PLQTLETNKPAGVGEKTGGPSTEVQNRQPPPNSKPLKAKTTKAERRALQEAQRAAKVSAK 220
           P    +++ P   GEK     T+ + R               AERRA QEA+RA K + K
Sbjct: 80  PGPGSQSSTP---GEKVPAGRTKAELR---------------AERRAKQEAERALKQARK 139

Query: 221 GEANKSGAAPGKAVKQPAAMKDSTAAPSVAPTDKRGGGDRTLDKERKKDAPPPRMQFDDK 280
           GE  + G  P              A+PS A     GG  + L +  + D P    +    
Sbjct: 140 GE--QGGPPP-------------QASPSTAGEAPAGG--KRLTEHTQADDPTLLRRL--- 199

Query: 281 SRVEKAKKRAVFNQTEARNRVELFRHLPQYEQGTQLPDLVSKFFQLDSIHPAIYKVGLQY 340
             V K++++ V  + +  ++V LF HLPQY +   L   +S       IHPA+ ++GLQY
Sbjct: 200 --VRKSERQQVPTRKDYGSKVSLFSHLPQYSRQNSLTQYMS--IPSSVIHPAMVRLGLQY 259

Query: 341 MARDICGGNARCIAMLHAFQQAIKDYSTPPQKSLGRDLTAKIGSYVSFFNECRPLSVSMG 400
               I G NARCIA+L A QQ I+DY+TPP + L RDL  K+  Y+ F  +CRPLS SM 
Sbjct: 260 SQGLISGSNARCIALLRALQQVIQDYTTPPNEELSRDLVNKLKPYICFLTQCRPLSASMY 319

Query: 401 NAIRFLKNRIAKLPLNLPESEVKATLCSDINRFINEKIIIADEVIVRHAATKIRDGDVLL 460
           NAI+FL   I  +     E E KA L +  +R++ EKI++A + I+R A+ KI +GDV+L
Sbjct: 320 NAIKFLNKEITGVSSTKREEEAKAELQAAADRYVQEKIVLAAQAILRFASKKISNGDVIL 379

Query: 461 TYGSSSAVEMLILYAHELGKEFRVVVVDSRPKQEGKALLRRLIARGLNCTYTHINAISYV 520
            YG SS V  ++  A   G++FRVVVVDSRP+ EG+ +LR L+  G+  +Y  I A SYV
Sbjct: 380 VYGCSSLVSRILQEAWSEGRKFRVVVVDSRPRLEGRHMLRFLVRAGVPASYLLIPAASYV 439

Query: 521 MHEVTRVFLGAAAVLSNGTVYSRVGTAAVAMVANAFHVPVLICCEAYKFHERVQLDSICF 580
           + EV++V LGA A+L+NG+V SRVGTA +A+VA A +VPVL+CCE YKF ERVQ D+   
Sbjct: 440 LPEVSKVLLGAHALLANGSVMSRVGTAQLALVARAHNVPVLVCCETYKFCERVQTDAFVS 499

Query: 581 NELGDPDTISKVPERKDINDLA---AKEQLQLLNLMYDATPSDYISMIVTDYGMIPPTSV 640
           NEL DPD +    ER D   LA   +   L+LLNL+YD TP + + +++T+ GMIP +SV
Sbjct: 500 NELDDPDDLQ--CERGDHVALANWQSHPSLRLLNLVYDVTPPELVDLVITELGMIPCSSV 517

Query: 641 PVIVR 643
           PV++R
Sbjct: 560 PVVLR 517

BLAST of Cucsa.198810 vs. Swiss-Prot
Match: EI2BD_HUMAN (Translation initiation factor eIF-2B subunit delta OS=Homo sapiens GN=EIF2B4 PE=1 SV=2)

HSP 1 Score: 335.9 bits (860), Expect = 1.0e-90
Identity = 219/546 (40.11%), Postives = 304/546 (55.68%), Query Frame = 1

Query: 101 LATSPMASPSSRTGDEELDMSEDSSNSVWHRRSGSGKIASSFPGVGIHVGAKSLENSQAV 160
           L   P A     T +E+L + ++       R+   G    + P  G    A S    Q  
Sbjct: 20  LPPGPGAVGREMTKEEKLQLRKEKKQQKKKRKEEKG----AEPETG---SAVSAAQCQVG 79

Query: 161 PLQTLETNKPAGVGEKTGGPSTEVQNRQPPPNSKPLKAKTTKAERRALQEAQRAAKVSAK 220
           P + L  +     G + G P  +V    P   SK       +AERRA QEA+RA K + K
Sbjct: 80  PTRELPES-----GIQLGTPREKV----PAGRSKA----ELRAERRAKQEAERALKQARK 139

Query: 221 GEANKSGAAPGKAVKQPAAMKDSTAAPSVAPTDKRGGGDRTLDKERKKDAPPPRMQFDD- 280
           GE    G  P KA            +PS A       G+     +R  + P    Q DD 
Sbjct: 140 GE---QGGPPPKA------------SPSTA-------GETPSGVKRLPEYP----QVDDL 199

Query: 281 --KSRVEKAKKRAVFNQTEARNRVELFRHLPQYEQGTQLPDLVSKFFQLDSIHPAIYKVG 340
             +  V+K +++ V  + +  ++V LF HLPQY +   L   +S       IHPA+ ++G
Sbjct: 200 LLRRLVKKPERQQVPTRKDYGSKVSLFSHLPQYSRQNSLTQFMS--IPSSVIHPAMVRLG 259

Query: 341 LQYMARDICGGNARCIAMLHAFQQAIKDYSTPPQKSLGRDLTAKIGSYVSFFNECRPLSV 400
           LQY    + G NARCIA+L A QQ I+DY+TPP + L RDL  K+  Y+SF  +CRPLS 
Sbjct: 260 LQYSQGLVSGSNARCIALLRALQQVIQDYTTPPNEELSRDLVNKLKPYMSFLTQCRPLSA 319

Query: 401 SMGNAIRFLKNRIAKLPLNLPESEVKATLCSDINRFINEKIIIADEVIVRHAATKIRDGD 460
           SM NAI+FL   I  +  +  E E K+ L + I+R++ EKI++A + I R A  KI +GD
Sbjct: 320 SMHNAIKFLNKEITSVGSSKREEEAKSELRAAIDRYVQEKIVLAAQAISRFAYQKISNGD 379

Query: 461 VLLTYGSSSAVEMLILYAHELGKEFRVVVVDSRPKQEGKALLRRLIARGLNCTYTHINAI 520
           V+L YG SS V  ++  A   G+ FRVVVVDSRP  EG+  LR L+  G+  +Y  I A 
Sbjct: 380 VILVYGCSSLVSRILQEAWTEGRRFRVVVVDSRPWLEGRHTLRSLVHAGVPASYLLIPAA 439

Query: 521 SYVMHEVTRVFLGAAAVLSNGTVYSRVGTAAVAMVANAFHVPVLICCEAYKFHERVQLDS 580
           SYV+ EV++V LGA A+L+NG+V SRVGTA +A+VA A +VPVL+CCE YKF ERVQ D+
Sbjct: 440 SYVLPEVSKVLLGAHALLANGSVMSRVGTAQLALVARAHNVPVLVCCETYKFCERVQTDA 499

Query: 581 ICFNELGDPDTIS-KVPERKDINDLAAKEQLQLLNLMYDATPSDYISMIVTDYGMIPPTS 640
              NEL DPD +  K  E   + +      L+LLNL+YD TP + + +++T+ GMIP +S
Sbjct: 500 FVSNELDDPDDLQCKRGEHVALANWQNHASLRLLNLVYDVTPPELVDLVITELGMIPCSS 517

Query: 641 VPVIVR 643
           VPV++R
Sbjct: 560 VPVVLR 517

BLAST of Cucsa.198810 vs. TrEMBL
Match: A0A0A0LPQ4_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_1G015850 PE=3 SV=1)

HSP 1 Score: 1291.9 bits (3342), Expect = 0.0e+00
Identity = 650/651 (99.85%), Postives = 651/651 (100.00%), Query Frame = 1

Query: 1   MDARRGSRIVNPKVRQVGFFTLDAPNSPDRSLSGPPNCLPSQQSLSPPTNSLSPVMIPPP 60
           MDARRGSRIVNPKVRQVGFFTLDAPNSPDRSLSGPPNCLPSQQSLSPPTNSLSPVMIPPP
Sbjct: 1   MDARRGSRIVNPKVRQVGFFTLDAPNSPDRSLSGPPNCLPSQQSLSPPTNSLSPVMIPPP 60

Query: 61  RHLSDNLASRSAVLPVPAPANWRNSTDQIPVGSYNPSETLLATSPMASPSSRTGDEELDM 120
           RHLSDNLASRSAVLPVPAPANWRNSTDQIPVGSYNPSETLLATSPMASPSSRTGDEELDM
Sbjct: 61  RHLSDNLASRSAVLPVPAPANWRNSTDQIPVGSYNPSETLLATSPMASPSSRTGDEELDM 120

Query: 121 SEDSSNSVWHRRSGSGKIASSFPGVGIHVGAKSLENSQAVPLQTLETNKPAGVGEKTGGP 180
           SEDSSNSVWHRRSGSGKIASSFPGVGIHVGAKSLENSQAVPLQTLETNKPAGVGEKTGGP
Sbjct: 121 SEDSSNSVWHRRSGSGKIASSFPGVGIHVGAKSLENSQAVPLQTLETNKPAGVGEKTGGP 180

Query: 181 STEVQNRQPPPNSKPLKAKTTKAERRALQEAQRAAKVSAKGEANKSGAAPGKAVKQPAAM 240
           STEVQNRQPPPNSKPLKAKTTKAERRALQEAQRAAKVSAKGEANKSGAAPGKAVKQPAAM
Sbjct: 181 STEVQNRQPPPNSKPLKAKTTKAERRALQEAQRAAKVSAKGEANKSGAAPGKAVKQPAAM 240

Query: 241 KDSTAAPSVAPTDKRGGGDRTLDKERKKDAPPPRMQFDDKSRVEKAKKRAVFNQTEARNR 300
           KDSTAAPSVAPTDKRGGGDRTLDKERKKDAPPPRMQFDDKSRVEKAKKRAVFNQTEARNR
Sbjct: 241 KDSTAAPSVAPTDKRGGGDRTLDKERKKDAPPPRMQFDDKSRVEKAKKRAVFNQTEARNR 300

Query: 301 VELFRHLPQYEQGTQLPDLVSKFFQLDSIHPAIYKVGLQYMARDICGGNARCIAMLHAFQ 360
           VELFRHLPQYEQGTQLPDLVSKFFQLDSIHPAIYKVGLQYMARDICGGNARCIAMLHAFQ
Sbjct: 301 VELFRHLPQYEQGTQLPDLVSKFFQLDSIHPAIYKVGLQYMARDICGGNARCIAMLHAFQ 360

Query: 361 QAIKDYSTPPQKSLGRDLTAKIGSYVSFFNECRPLSVSMGNAIRFLKNRIAKLPLNLPES 420
           Q+IKDYSTPPQKSLGRDLTAKIGSYVSFFNECRPLSVSMGNAIRFLKNRIAKLPLNLPES
Sbjct: 361 QSIKDYSTPPQKSLGRDLTAKIGSYVSFFNECRPLSVSMGNAIRFLKNRIAKLPLNLPES 420

Query: 421 EVKATLCSDINRFINEKIIIADEVIVRHAATKIRDGDVLLTYGSSSAVEMLILYAHELGK 480
           EVKATLCSDINRFINEKIIIADEVIVRHAATKIRDGDVLLTYGSSSAVEMLILYAHELGK
Sbjct: 421 EVKATLCSDINRFINEKIIIADEVIVRHAATKIRDGDVLLTYGSSSAVEMLILYAHELGK 480

Query: 481 EFRVVVVDSRPKQEGKALLRRLIARGLNCTYTHINAISYVMHEVTRVFLGAAAVLSNGTV 540
           EFRVVVVDSRPKQEGKALLRRLIARGLNCTYTHINAISYVMHEVTRVFLGAAAVLSNGTV
Sbjct: 481 EFRVVVVDSRPKQEGKALLRRLIARGLNCTYTHINAISYVMHEVTRVFLGAAAVLSNGTV 540

Query: 541 YSRVGTAAVAMVANAFHVPVLICCEAYKFHERVQLDSICFNELGDPDTISKVPERKDIND 600
           YSRVGTAAVAMVANAFHVPVLICCEAYKFHERVQLDSICFNELGDPDTISKVPERKDIND
Sbjct: 541 YSRVGTAAVAMVANAFHVPVLICCEAYKFHERVQLDSICFNELGDPDTISKVPERKDIND 600

Query: 601 LAAKEQLQLLNLMYDATPSDYISMIVTDYGMIPPTSVPVIVREYRREHLWI 652
           LAAKEQLQLLNLMYDATPSDYISMIVTDYGMIPPTSVPVIVREYRREHLWI
Sbjct: 601 LAAKEQLQLLNLMYDATPSDYISMIVTDYGMIPPTSVPVIVREYRREHLWI 651

BLAST of Cucsa.198810 vs. TrEMBL
Match: A0A0A0LSL4_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_1G015840 PE=3 SV=1)

HSP 1 Score: 1112.4 bits (2876), Expect = 0.0e+00
Identity = 573/654 (87.61%), Postives = 595/654 (90.98%), Query Frame = 1

Query: 1   MDARRGSRIVNPKVRQVGFFTLDAPNSPDRSLSGPPNCLPSQQSLSPPTNSLSPVMIPPP 60
           MDARRGSR+VNPK+RQVGFFTLD PN P RSLSGP +C PSQQSLSPP+NSLSPVMIPPP
Sbjct: 1   MDARRGSRVVNPKIRQVGFFTLDPPNPPGRSLSGPADCTPSQQSLSPPSNSLSPVMIPPP 60

Query: 61  RHLSDNLASRSAVLPVPAPANWRNSTDQIPVGSYNPSETLLATSPMASPSSRTGDEELDM 120
           RHLSDNLASRSAV P+P PANWR+S D I VGSYNPSE LLATSP    S R GDEELDM
Sbjct: 61  RHLSDNLASRSAVFPLPPPANWRDSADHIHVGSYNPSEMLLATSP----SGRIGDEELDM 120

Query: 121 SEDSSNSVWHRRSGSGKIASSFPGVGIHVGAKSLENSQAVPLQTLETNKPAGVGEKTGGP 180
           SEDS  SV +RRSGSGKIASSFPGVGI++ AK+ E SQA+   T+E NK  GV EK GG 
Sbjct: 121 SEDSFTSVPYRRSGSGKIASSFPGVGIYMAAKASETSQAMVPNTVEANKSVGVAEKNGGA 180

Query: 181 STEVQNRQPPPNSKPLKAKTTKAERRALQEAQRAAKVSAKGEANKSGAAPGKAVKQPAAM 240
           STE+QNRQPP NSKP KAKTTKAERRALQEAQRAAK    GEA KS  APGKAVKQ + M
Sbjct: 181 STELQNRQPPSNSKPQKAKTTKAERRALQEAQRAAK----GEAKKSSNAPGKAVKQSSTM 240

Query: 241 KDSTAAPSVAPTDKRGGGDRTLDKERKKDAPPPRMQFDDKSRVEKAKKRAVFNQTEARNR 300
           +DSTAAPSVA TDKRGG DRT DKERKKDAPPPRMQFDDKSRVEKAKKRAVFNQTEARNR
Sbjct: 241 RDSTAAPSVASTDKRGG-DRTHDKERKKDAPPPRMQFDDKSRVEKAKKRAVFNQTEARNR 300

Query: 301 VELFRHLPQYEQGTQLPDLVSKFFQLDSIHPAIYKVGLQYMARDICGGNARCIAMLHAFQ 360
           VELFRHLPQYEQGTQLPDLVSKFFQLD IHPAIYKVGLQYMA DICGGNARCIAMLHAFQ
Sbjct: 301 VELFRHLPQYEQGTQLPDLVSKFFQLDPIHPAIYKVGLQYMAGDICGGNARCIAMLHAFQ 360

Query: 361 QAIKDYSTPPQKSLGRDLTAKIGSYVSFFNECRPLSVSMGNAIRFLKNRIAKLPLNLPES 420
           QAIKDYSTPPQKSLGRDLT KIG YVSFFNECRPLSVSMGNAIRFLKN IAKLPLNLPES
Sbjct: 361 QAIKDYSTPPQKSLGRDLTTKIGGYVSFFNECRPLSVSMGNAIRFLKNLIAKLPLNLPES 420

Query: 421 EVKATLCSDINRFINEKIIIADEVIVRHAATKIRDGDVLLTYGSSSAVEMLILYAHELGK 480
           EVKATLCSDI+RFINEKIIIADEVIVRHAATKIRDGDVLLTYGSS AVEML+LYAHELGK
Sbjct: 421 EVKATLCSDIDRFINEKIIIADEVIVRHAATKIRDGDVLLTYGSSCAVEMLLLYAHELGK 480

Query: 481 EFRVVVVDSRPKQEGKALLRRLIARGLNCTYTHINAISYVMHEVTRVFLGAAAVLSNGTV 540
           EFRVVVVDSRPK EG+ALLRRLI RGL+CTYTHINAISY+MH+VTRVFLGAAAVLSNGTV
Sbjct: 481 EFRVVVVDSRPKLEGQALLRRLIGRGLSCTYTHINAISYMMHKVTRVFLGAAAVLSNGTV 540

Query: 541 YSRVGTAAVAMVANAFHVPVLICCEAYKFHERVQLDSICFNELGDPDTISKVPERKDIN- 600
           YSRVGTAAVAMVANAFHVPVLICCEAYKFHERVQLDSICFNELGDPD ISKVP RKDIN 
Sbjct: 541 YSRVGTAAVAMVANAFHVPVLICCEAYKFHERVQLDSICFNELGDPDAISKVPGRKDINH 600

Query: 601 --DLAAKEQLQLLNLMYDATPSDYISMIVTDYGMIPPTSVPVIVREYRREHLWI 652
             +LAAKEQLQLLNLMYDATPSDYISMIVTDYGMIPPTSVPVIVREYRREHLWI
Sbjct: 601 LENLAAKEQLQLLNLMYDATPSDYISMIVTDYGMIPPTSVPVIVREYRREHLWI 645

BLAST of Cucsa.198810 vs. TrEMBL
Match: W9S6A9_9ROSA (Translation initiation factor eIF-2B subunit delta OS=Morus notabilis GN=L484_022286 PE=3 SV=1)

HSP 1 Score: 884.4 bits (2284), Expect = 8.4e-254
Identity = 470/666 (70.57%), Postives = 539/666 (80.93%), Query Frame = 1

Query: 1   MDARRGSRIVN-PKVRQVGFFTLDAPNSPDRSLSGPPNCLPSQQSLSPPTNSLSPVMIPP 60
           MDARR SR V+ PKVR VGFFT +AP  P R+ SGPP       S SP +NS SPVMIPP
Sbjct: 1   MDARRSSRAVSDPKVRHVGFFTPNAPPLPSRTQSGPPAAGSPPLSESPASNSFSPVMIPP 60

Query: 61  PRHLSDNLASRSAVLPVPAPANWRNST-DQIPVGSYNPSETLLATSPMASPSSRTGDEEL 120
           PRHLSD +A R+A +PVP  A  R +  D +PVGSYNPSE+LL TSP+ASPSS  GD EL
Sbjct: 61  PRHLSD-VAERTAAVPVPDSAYRRTAAGDHVPVGSYNPSESLLGTSPVASPSSVAGDGEL 120

Query: 121 DMSEDSSNSVWHRRSGSGKIASSFPGVGIHVG-AKSLENSQAVPLQTLETN---KPAGVG 180
           + S +SS S WHR   SGK ASSFP  G  +  AK  ENS          +   KP  V 
Sbjct: 121 EFSGESSAS-WHRWRDSGKFASSFPSGGFELTMAKGSENSATKGKNLAAASNAKKPLDVP 180

Query: 181 EKTGGPSTEVQNRQPPPNSKPLKAKTTKAERRALQEAQRAAKVSAKGEANKS-----GAA 240
           EK+GG  TEVQN +  P SKPLKAK+TKAERRALQEAQRAAK + K E +KS     G A
Sbjct: 181 EKSGGVVTEVQN-ELQPTSKPLKAKSTKAERRALQEAQRAAKAATK-EGSKSATVSRGVA 240

Query: 241 PGKAVKQPAAMKD-STAAPSVAPTDKRGGGDRTLDKERKKDAPPPRMQFDDKSRVEKAKK 300
           P K VKQP+  KD S+ APSVA ++K+GG DR  +KERKKD PPPRMQFDDK+RVEKAKK
Sbjct: 241 PSKPVKQPSQRKDASSVAPSVAASEKKGG-DRAPEKERKKDVPPPRMQFDDKNRVEKAKK 300

Query: 301 RAVFNQTEARNRVELFRHLPQYEQGTQLPDLVSKFFQLDSIHPAIYKVGLQYMARDICGG 360
           R+VF+QTEARNRVELFRHLPQYE GTQLPDL SKFFQL+ +HPAIYKVGLQY+A DICGG
Sbjct: 301 RSVFHQTEARNRVELFRHLPQYEHGTQLPDLESKFFQLEPMHPAIYKVGLQYLAGDICGG 360

Query: 361 NARCIAMLHAFQQAIKDYSTPPQKSLGRDLTAKIGSYVSFFNECRPLSVSMGNAIRFLKN 420
           N RC+AML AF++ I+DYSTPP+K+L RDLTAKI SYVSFF ECRP+S+SMGNAIRF+K+
Sbjct: 361 NGRCVAMLKAFEEVIRDYSTPPEKALSRDLTAKISSYVSFFTECRPISISMGNAIRFIKS 420

Query: 421 RIAKLPLNLPESEVKATLCSDINRFINEKIIIADEVIVRHAATKIRDGDVLLTYGSSSAV 480
           RIAKLPL L ESE KA LC+DIN FINEKI++AD VIV+HAA+K++DGDVLLTYGSS  V
Sbjct: 421 RIAKLPLFLSESEAKAALCADINSFINEKILLADRVIVKHAASKVKDGDVLLTYGSSCVV 480

Query: 481 EMLILYAHELGKEFRVVVVDSRPKQEGKALLRRLIARGLNCTYTHINAISYVMHEVTRVF 540
           EM++L+AHELGK+FRVV+VDSRPK EG+ALL RL+A+GL+CTYTHINA+SYVMHEVT+V 
Sbjct: 481 EMILLHAHELGKQFRVVIVDSRPKFEGQALLCRLVAKGLSCTYTHINAVSYVMHEVTKVL 540

Query: 541 LGAAAVLSNGTVYSRVGTAAVAMVANAFHVPVLICCEAYKFHERVQLDSICFNELGDPDT 600
           LGAA+VLSNGTVYSRVGTA+VAMVA+AF VPVLICCEAYKFHERVQLDSIC NELGD D 
Sbjct: 541 LGAASVLSNGTVYSRVGTASVAMVAHAFCVPVLICCEAYKFHERVQLDSICSNELGDTDA 600

Query: 601 ISKVPERKDIN---DLAAKEQLQLLNLMYDATPSDYISMIVTDYGMIPPTSVPVIVREYR 652
           I+KVP R D+N   +LA KE LQLLNLMYDATPSDY+SMI+TDYGMIPPTSVPVIVREYR
Sbjct: 601 IAKVPGRMDVNYLENLAKKENLQLLNLMYDATPSDYVSMIITDYGMIPPTSVPVIVREYR 660

BLAST of Cucsa.198810 vs. TrEMBL
Match: M5WXG3_PRUPE (Uncharacterized protein OS=Prunus persica GN=PRUPE_ppa002668mg PE=3 SV=1)

HSP 1 Score: 879.4 bits (2271), Expect = 2.7e-252
Identity = 469/663 (70.74%), Postives = 535/663 (80.69%), Query Frame = 1

Query: 1   MDARRGSR-IVNPKVRQVGFFTLDAPNSPDRSLSGPPNCLPSQQSLSPPTNSLSPVMIPP 60
           MDARR SR +++PKVRQVGFFT +A  +P R+LSGPP       S SP +NSL PVMIPP
Sbjct: 1   MDARRASRTVIDPKVRQVGFFTPNAETAPSRTLSGPPIASSPPLSSSPASNSLYPVMIPP 60

Query: 61  PRHLSDNLASRSAVLPVPAPANWRNST-DQIPVGSYNPSETLLATSPM-ASPSSRTGDEE 120
            RHLSD +A R+A LPVP  A+ R    +++PVGSYNPSE+LL TSP+ ASPSSR GD  
Sbjct: 61  SRHLSDTIAIRTAALPVPESASRRKEAGERVPVGSYNPSESLLGTSPVTASPSSRVGDAT 120

Query: 121 LDMSEDSSNSVWHRRSGSGKIASSFPGVGIHVGAKSLENSQAVPLQTLETNKPAGVGEKT 180
           L+ S++SS   W+RRS SGK+ASSFPG G                 +     P  V EK+
Sbjct: 121 LEFSDESSVR-WYRRSDSGKLASSFPGGGF---------------DSATVKAPLEVPEKS 180

Query: 181 GGPSTEVQNRQPPPNSKPLKAKTT-KAERRALQEAQRAAKVSAKGEANKS-----GAAPG 240
           G   ++ Q ++ P NS+PLK+KTT KAERRALQEAQRAAK +AK E  K      G APG
Sbjct: 181 GEEVSKEQ-KEVPSNSEPLKSKTTTKAERRALQEAQRAAKAAAKAEGKKPAAVTRGGAPG 240

Query: 241 KAVKQPAAMKDST-AAPSVAPTDKRGGGDRTLDKERKKDAPPPRMQFDDKSRVEKAKKRA 300
           K  KQ    +D    A SVA +DK+GG DR  +KERKKD PPPRMQFDDK+RV+KAK+RA
Sbjct: 241 KPAKQSLQKRDPPPVASSVAASDKKGG-DRPPEKERKKDVPPPRMQFDDKNRVDKAKRRA 300

Query: 301 VFNQTEARNRVELFRHLPQYEQGTQLPDLVSKFFQLDSIHPAIYKVGLQYMARDICGGNA 360
           V NQTEA+NRVELFRHLPQYE GTQLPDL SKFFQLD +HPAIYKVGLQY+A +I GGNA
Sbjct: 301 VVNQTEAKNRVELFRHLPQYEHGTQLPDLESKFFQLDPMHPAIYKVGLQYLAGEISGGNA 360

Query: 361 RCIAMLHAFQQAIKDYSTPPQKSLGRDLTAKIGSYVSFFNECRPLSVSMGNAIRFLKNRI 420
           RCIAML AFQ+AI+DY+TPP+K+L RDLTAKI SYVSFF ECRPLS+SMGNAIRFLK+RI
Sbjct: 361 RCIAMLQAFQEAIRDYTTPPEKTLVRDLTAKISSYVSFFIECRPLSISMGNAIRFLKSRI 420

Query: 421 AKLPLNLPESEVKATLCSDINRFINEKIIIADEVIVRHAATKIRDGDVLLTYGSSSAVEM 480
           AKLPL L ESE K+TLCS+I+RFINEKII+AD+VIVRHAATK+RDGDVLLTYGSS  VEM
Sbjct: 421 AKLPLTLSESEAKSTLCSEIDRFINEKIILADKVIVRHAATKVRDGDVLLTYGSSCVVEM 480

Query: 481 LILYAHELGKEFRVVVVDSRPKQEGKALLRRLIARGLNCTYTHINAISYVMHEVTRVFLG 540
           ++LYAHELGK+FRVVVVDSRPK EG+ALLRRL+A+GL+CTYTHINA+SY+MHEVTRVFLG
Sbjct: 481 ILLYAHELGKQFRVVVVDSRPKLEGQALLRRLVAKGLSCTYTHINAVSYIMHEVTRVFLG 540

Query: 541 AAAVLSNGTVYSRVGTAAVAMVANAFHVPVLICCEAYKFHERVQLDSICFNELGDPDTIS 600
           AA+VLSNGTVYSRVGTA VAMVA+AF VPVLICCEAYKFHERVQLDSIC NELGDPD I+
Sbjct: 541 AASVLSNGTVYSRVGTACVAMVAHAFCVPVLICCEAYKFHERVQLDSICSNELGDPDAIA 600

Query: 601 KVPERKDINDL---AAKEQLQLLNLMYDATPSDYISMIVTDYGMIPPTSVPVIVREYRRE 651
           KV  R D+N L   A KE LQLLNLMYDATPSDYISMIVTDYGMIPPTSVPVIVREY RE
Sbjct: 601 KVSGRTDVNYLDNWANKENLQLLNLMYDATPSDYISMIVTDYGMIPPTSVPVIVREYGRE 645

BLAST of Cucsa.198810 vs. TrEMBL
Match: A0A067JJB6_JATCU (Uncharacterized protein OS=Jatropha curcas GN=JCGZ_05844 PE=3 SV=1)

HSP 1 Score: 839.0 bits (2166), Expect = 4.0e-240
Identity = 465/670 (69.40%), Postives = 536/670 (80.00%), Query Frame = 1

Query: 1   MDARRGSRIVNPKVRQVGFFTLDAPNSPDRSLSGPPNCLPSQQSLSPPTNSLSPVMIPPP 60
           M+ARRG R V PKVRQVGFFT + P  P RS SGPP+    Q + SP +NSLSPVMIPPP
Sbjct: 1   MEARRGWRAV-PKVRQVGFFTPNEPLPPTRSQSGPPDSNSPQLANSPASNSLSPVMIPPP 60

Query: 61  RHLSDNLASRS-AVLPVP-APANWRNST--DQIP-VGSYNPSETLLATSPMASPSSRTGD 120
           RH+SDNLA R+ + LPVP APA  R+ T  D +  VGSYNP+E+LL  SP   PS+R  D
Sbjct: 61  RHMSDNLAHRATSPLPVPEAPAFRRSITGGDHVTLVGSYNPTESLLGQSP---PSARIVD 120

Query: 121 EELDMSEDSSNSVWHRRSGSGKIASSFPGVGIHVGAKSLENSQAVPLQTLETNKPAGVGE 180
            E   SE+SS   W RRS S K ASSFP  G  +   SL++S+ + +Q     KP G  E
Sbjct: 121 GEF--SEESSPG-WLRRSNSAKFASSFPSGGFDL--TSLKSSENLEVQE---KKPVGGPE 180

Query: 181 KTGGPSTEVQNRQPPPNSKPLKAKTTKAERRALQEAQRAAKVSAK--GEA-------NKS 240
           K GG   E+QN +  P+SKPLKAKTT+AERRALQE+QRAAK +AK  GEA       ++ 
Sbjct: 181 KGGGV-VELQN-ELQPSSKPLKAKTTRAERRALQESQRAAKAAAKATGEAVGKPAILSEG 240

Query: 241 GAAPGKAVKQPAAMKDST--AAPSVAPTDKRGGGDRTLDKERKKDAPPPRMQFDDKSRVE 300
            AA   A +QP+  KD T  AA S+A +D++G  DR  +KER+++ P PRMQFDD++RVE
Sbjct: 241 AAASKPAKQQPSQKKDVTPPAASSIAASDRKGV-DRPPEKERRREVPAPRMQFDDRTRVE 300

Query: 301 KAKKRAVFNQTEARNRVELFRHLPQYEQGTQLPDLVSKFFQLDSIHPAIYKVGLQYMARD 360
           KAKKRAV NQTEARNRVELFRHLPQYE GTQLPDL SK FQLD +HPAIYK GL+Y+A D
Sbjct: 301 KAKKRAVVNQTEARNRVELFRHLPQYEHGTQLPDLESKIFQLDPMHPAIYKAGLKYLAGD 360

Query: 361 ICGGNARCIAMLHAFQQAIKDYSTPPQKSLGRDLTAKIGSYVSFFNECRPLSVSMGNAIR 420
           I GGNARCIAML AFQ+ IKDYSTPP+KSL RDLTAK+GSY+SF  ECRPLS+SMGNAIR
Sbjct: 361 IIGGNARCIAMLQAFQEVIKDYSTPPEKSLTRDLTAKLGSYISFLIECRPLSMSMGNAIR 420

Query: 421 FLKNRIAKLPLNLPESEVKATLCSDINRFINEKIIIADEVIVRHAATKIRDGDVLLTYGS 480
           FLK+RIAKLPL   ESE KA+L SDI+RFINEKI++AD+VIVRHAA+KIRDGDVLLTYGS
Sbjct: 421 FLKSRIAKLPLTFSESEAKASLRSDIDRFINEKIVLADKVIVRHAASKIRDGDVLLTYGS 480

Query: 481 SSAVEMLILYAHELGKEFRVVVVDSRPKQEGKALLRRLIARGLNCTYTHINAISYVMHEV 540
           S  VEM++LYAHELGK+FRVV+VDSRPK EG+ALLR L+A+GLNCTYTHINA+SY+MHEV
Sbjct: 481 SCVVEMILLYAHELGKQFRVVIVDSRPKLEGRALLRTLVAKGLNCTYTHINAVSYIMHEV 540

Query: 541 TRVFLGAAAVLSNGTVYSRVGTAAVAMVANAFHVPVLICCEAYKFHERVQLDSICFNELG 600
           TRVFLGA++VLSNGTVYSRVGTA VAMVA+AFHVPVLICCEAYKFHERVQLDSIC NELG
Sbjct: 541 TRVFLGASSVLSNGTVYSRVGTACVAMVAHAFHVPVLICCEAYKFHERVQLDSICSNELG 600

Query: 601 DPDTISKVPERKDIN---DLAAKEQLQLLNLMYDATPSDYISMIVTDYGMIPPTSVPVIV 652
           DP+ I+KV  R D+N    LA KE LQLLNLMYDATPS+YISMIVTDYGMIPPTSVPVIV
Sbjct: 601 DPNAIAKVSGRTDVNYLDTLANKENLQLLNLMYDATPSEYISMIVTDYGMIPPTSVPVIV 655

BLAST of Cucsa.198810 vs. TAIR10
Match: AT5G38640.1 (AT5G38640.1 NagB/RpiA/CoA transferase-like superfamily protein)

HSP 1 Score: 730.3 bits (1884), Expect = 1.0e-210
Identity = 411/679 (60.53%), Postives = 481/679 (70.84%), Query Frame = 1

Query: 1   MDARRGSR-IVNPKVRQVGFFTLDAPNSPDRSLSGPP----NCLPSQQSLSPPTNSLSPV 60
           M++RR  R +V+PKVRQVGFFT   P+S  +     P    +   S  ++SP  NSLSPV
Sbjct: 1   MESRRNPRAVVDPKVRQVGFFTSSQPDSSTQQQQPDPIAATSASSSSSTISPSGNSLSPV 60

Query: 61  MIPPPR-HLSDNLASRSAVLPVPAPANWRNSTDQIPVGSYNPSETLLATSPMASPSSRTG 120
           MIPPPR + SD    R AV    A A                           SPSS + 
Sbjct: 61  MIPPPRLNSSDTFPLRVAVAGAAASA--------------------------VSPSSFSY 120

Query: 121 DEELDMSEDSSNSVWHRRS------GSGKIASSFPGVGIHVGAKSLENSQAVPLQTLET- 180
               D  + S+ +   R S       S  +ASS PG+G+   A +   S +VP   L T 
Sbjct: 121 SSRRDFPDGSTTASPGRHSRGSFVKSSVPVASSLPGIGMDSMAAA--KSSSVPASGLTTV 180

Query: 181 ---NKPAGVGEKTGGPSTEVQNRQPPPNSKPLKAKTTKAERRALQEAQRAAKVSAKGEAN 240
                P G+ EK  G   EVQ+ Q     KPLK KTTKAERRA+QEAQRAAK +AK +  
Sbjct: 181 SVVTMPPGLSEKASG--MEVQSDQ----KKPLKEKTTKAERRAIQEAQRAAKAAAKADGG 240

Query: 241 K-----SGAAPGKAVKQPAAMKDSTAAPSVA----PTDKRGGGDRTLDKERKKDAPPPRM 300
           K     S +    +VK     K ++    VA      +K+GG     +K+RKKDAP PRM
Sbjct: 241 KVAPVSSASVASTSVKAAKPAKATSQKNDVAVATGAAEKKGGPS---EKDRKKDAPHPRM 300

Query: 301 QFDDKSRVEKAKKRAVFNQTEARNRVELFRHLPQYEQGTQLPDLVSKFFQLDSIHPAIYK 360
           Q+DD+SRV KAK+RAV  QTEA+NRVELFRHLPQYE GTQLPDL +KFFQLD +HPA+YK
Sbjct: 301 QYDDESRVVKAKRRAVVKQTEAKNRVELFRHLPQYEHGTQLPDLETKFFQLDPMHPAVYK 360

Query: 361 VGLQYMARDICGGNARCIAMLHAFQQAIKDYSTPPQKSLGRDLTAKIGSYVSFFNECRPL 420
           VGLQY++ DI GGNARCIAML AFQ+ +KDYSTPP+KSL RD+TAKI SYVSF  ECRPL
Sbjct: 361 VGLQYLSGDISGGNARCIAMLQAFQEVVKDYSTPPEKSLNRDMTAKISSYVSFLIECRPL 420

Query: 421 SVSMGNAIRFLKNRIAKLPLNLPESEVKATLCSDINRFINEKIIIADEVIVRHAATKIRD 480
           S+SMGNAIRF+KNRIAKLP+ L ESE KATL SDI RFINEKII+AD VIV+HA TKIRD
Sbjct: 421 SISMGNAIRFVKNRIAKLPITLSESEAKATLQSDIERFINEKIIVADTVIVKHAVTKIRD 480

Query: 481 GDVLLTYGSSSAVEMLILYAHELGKEFRVVVVDSRPKQEGKALLRRLIARGLNCTYTHIN 540
           GDVLLTYGS +AVEM+IL+AHEL K+FRV+VVDSRPK EG+ LLRRLI RG+NCTYTHIN
Sbjct: 481 GDVLLTYGSPTAVEMVILHAHELRKKFRVLVVDSRPKLEGQLLLRRLIKRGINCTYTHIN 540

Query: 541 AISYVMHEVTRVFLGAAAVLSNGTVYSRVGTAAVAMVANAFHVPVLICCEAYKFHERVQL 600
           AISY+MHEVT+VFLGA++VLSNGTVYSRVGTA VAMVANAF VPVL+CCEAYKFHERVQL
Sbjct: 541 AISYIMHEVTKVFLGASSVLSNGTVYSRVGTACVAMVANAFRVPVLVCCEAYKFHERVQL 600

Query: 601 DSICFNELGDPDTISKVPERKDIN---DLAAKEQLQLLNLMYDATPSDYISMIVTDYGMI 652
           DSIC NELGDP+ IS+V  R+DIN    L     LQ LNLMYDATPSDYISMI+TDYGM+
Sbjct: 601 DSICSNELGDPNAISEVHGREDINYLDGLTNNANLQFLNLMYDATPSDYISMIITDYGMV 642

BLAST of Cucsa.198810 vs. TAIR10
Match: AT1G48970.1 (AT1G48970.1 NagB/RpiA/CoA transferase-like superfamily protein)

HSP 1 Score: 706.1 bits (1821), Expect = 2.1e-203
Identity = 389/677 (57.46%), Postives = 482/677 (71.20%), Query Frame = 1

Query: 1   MDARRGSRIVNPKVRQVGFFTLDAP------NSPDRSLSGPPNCLPSQQSLS--PPTNSL 60
           MD RRG+  V PKVR+VGFFT   P        P+RS SGP +   S   LS  P  N +
Sbjct: 1   MDTRRGALAV-PKVRRVGFFTSIEPPPESSLQRPNRSQSGPVDATTSSSPLSDSPSGNLI 60

Query: 61  SPVMIPPPRHLSDNLASR-----SAVLPVPAPANWRN----STDQIPVGSYNPSETLLAT 120
           SPV+IPP RH SDNL SR     +A +PVP PA +R         + VGSYNP ++LL T
Sbjct: 61  SPVLIPPSRHHSDNLTSRVAAAAAAPVPVPGPAAFRRYLTPERTLLHVGSYNPPDSLLGT 120

Query: 121 SPMASPSSRTGDEELDMSEDSSNSVWHRRSGSGKIASSFP--GVGIHVGAKSLENSQAVP 180
           S   SPSS  G     +SEDS++    +RS S K+++S P  G  + +  ++ + S+   
Sbjct: 121 S---SPSSNGG-----LSEDSASLFGFQRSDSTKLSASLPNGGFDLTLAVRAPQESETKI 180

Query: 181 LQTLETNKPAGVGEKTGGPSTEVQNRQPPPNSKPLKAKTTKAERRALQEAQRAAKVSAKG 240
             T  +     + E +G   +     Q     K LK KTTKAERRA+QEAQRAAK +AKG
Sbjct: 181 ATTSASQGKKKIAEVSGKSESATTKPQKEKEPKALKDKTTKAERRAIQEAQRAAKAAAKG 240

Query: 241 E----ANKSGAA-PGKAVKQPAAMKDSTAAPSVAPTDKRGGGDRTLDKERKKDAPPPRMQ 300
           E    A++SG A PGKA  +P   K+     S + ++K       ++KE++ D P  +MQ
Sbjct: 241 EGSRRADESGRANPGKAAIKPQPKKERLPVTS-SVSEKTAVA---VEKEKRMDVPQTQMQ 300

Query: 301 FDDKSRVEKAKKRAVFNQTEARNRVELFRHLPQYEQGTQLPDLVSKFFQLDSIHPAIYKV 360
           +DDKSRV+KAK+RAV  QTE++N+VELF HLPQYE+  QLP+L S FF LDSIH A+YKV
Sbjct: 301 YDDKSRVDKAKRRAVVEQTESKNKVELFLHLPQYERSNQLPNLSSNFFTLDSIHHAVYKV 360

Query: 361 GLQYMARDICGGNARCIAMLHAFQQAIKDYSTPPQKSLGRDLTAKIGSYVSFFNECRPLS 420
           GLQ++A DI G NARCIAML AFQ+AI+DYSTPP K L  DLTAKI  YVSF  ECRPLS
Sbjct: 361 GLQHLAGDISGDNARCIAMLQAFQEAIEDYSTPPMKDLTMDLTAKINGYVSFLIECRPLS 420

Query: 421 VSMGNAIRFLKNRIAKLPLNLPESEVKATLCSDINRFINEKIIIADEVIVRHAATKIRDG 480
           +SMGNAIRFLKN+I KLP+NL ESE K++LCSDI RFI+EK IIAD+ IV+HA TKIRDG
Sbjct: 421 MSMGNAIRFLKNQIRKLPVNLSESEAKSSLCSDIGRFIDEKTIIADKAIVQHAVTKIRDG 480

Query: 481 DVLLTYGSSSAVEMLILYAHELGKEFRVVVVDSRPKQEGKALLRRLIARGLNCTYTHINA 540
           +VLLTYG S  VEM++LYAHE+GK+FRVV+VDSRP  EG+ LLRRL+ RGL+CTYTHINA
Sbjct: 481 EVLLTYGFSCVVEMILLYAHEIGKKFRVVIVDSRPNLEGQKLLRRLVTRGLDCTYTHINA 540

Query: 541 ISYVMHEVTRVFLGAAAVLSNGTVYSRVGTAAVAMVANAFHVPVLICCEAYKFHERVQLD 600
           ISY+M E TRVFLGA+++ SNGT+Y+RVGT+ +AMVANAF VPV++CCEAYKFHERV LD
Sbjct: 541 ISYIMREATRVFLGASSIYSNGTLYARVGTSCIAMVANAFSVPVIVCCEAYKFHERVLLD 600

Query: 601 SICFNELGDPDTISKVP----ERKDINDLAAKEQLQLLNLMYDATPSDYISMIVTDYGMI 650
           SIC NELGDPD ++ +P      K    +   + LQ LNLMYD+TPS+YISMIV+DYGMI
Sbjct: 601 SICSNELGDPDAVANIPSFRTNAKHSKTMDNNKNLQFLNLMYDSTPSEYISMIVSDYGMI 660

BLAST of Cucsa.198810 vs. TAIR10
Match: AT2G44070.1 (AT2G44070.1 NagB/RpiA/CoA transferase-like superfamily protein)

HSP 1 Score: 493.0 bits (1268), Expect = 2.8e-139
Identity = 244/325 (75.08%), Postives = 282/325 (86.77%), Query Frame = 1

Query: 329 IHPAIYKVGLQYMARDICGGNARCIAMLHAFQQAIKDYSTPPQKSLGRDLTAKIGSYVSF 388
           +H A+YKVGL Y++ DI GGNARCIAML AFQ+ +KDYSTPP+ +L RD+TA+I SYVSF
Sbjct: 1   MHSAVYKVGLHYLSWDISGGNARCIAMLQAFQEVVKDYSTPPENTLNRDMTAQISSYVSF 60

Query: 389 FNECRPLSVSMGNAIRFLKNRIAKLPLNLPESEVKATLCSDINRFINEKIIIADEVIVRH 448
             ECRPLS+SMGNAIRF+KNRIAKLP+ L ESE KA+L SDI RFINEKII AD VIV H
Sbjct: 61  LIECRPLSISMGNAIRFVKNRIAKLPITLSESEAKASLHSDIERFINEKIIGADTVIVEH 120

Query: 449 AATKIRDGDVLLTYGSSSAVEMLILYAHELGKEFRVVVVDSRPKQEGKALLRRLIARGLN 508
           A TKIRDGDVLLTYGSS+ +EM++++AHELGK+FRV VVDSRPK +GK LLRRLI RG+N
Sbjct: 121 AVTKIRDGDVLLTYGSSTVIEMILVHAHELGKKFRVFVVDSRPKLQGKLLLRRLIKRGIN 180

Query: 509 CTYTHINAISYVMHEVTRVFLGAAAVLSNGTVYSRVGTAAVAMVANAFHVPVLICCEAYK 568
           CTYTHI AISY+MH+VT+VFLGA++V SNGTVYSRVGTA VAMVANAF VPVL+CCEAYK
Sbjct: 181 CTYTHITAISYIMHQVTKVFLGASSVFSNGTVYSRVGTACVAMVANAFRVPVLVCCEAYK 240

Query: 569 FHERVQLD-SICFNELGDPDTISKVPERKDI---NDLAAKEQLQLLNLMYDATPSDYISM 628
           FHE+VQLD SIC NELGDP+ ISKV  R+DI   N L    +L+ LNLMYDATPSDYISM
Sbjct: 241 FHEKVQLDYSICSNELGDPNAISKVHGREDINYLNGLTNNAKLKFLNLMYDATPSDYISM 300

Query: 629 IVTDYGMIPPTSVPVIVREYRREHL 650
           I+TDYGM+PPTSVPVIVREY++EHL
Sbjct: 301 IITDYGMVPPTSVPVIVREYQKEHL 325

BLAST of Cucsa.198810 vs. TAIR10
Match: AT3G07300.3 (AT3G07300.3 NagB/RpiA/CoA transferase-like superfamily protein)

HSP 1 Score: 98.6 bits (244), Expect = 1.5e-20
Identity = 67/226 (29.65%), Postives = 108/226 (47.79%), Query Frame = 1

Query: 421 EVKATLCSDINRFINEKIIIADEVIVRHAATKIRDGDVLLTYGSSSAVEMLILYAHELGK 480
           ++K  +   +N+ I E I    E I   A   I   +V+LT GSS  V   +  A E  +
Sbjct: 177 KLKHDVIEGVNQLIQE-IAGCHEQIAEQAIEHIHQNEVILTLGSSRTVLEFLCAAKEKKR 236

Query: 481 EFRVVVVDSRPKQEGKALLRRLIARGLNCTYTHINAISYVMHEVTRVFLGAAAVLSNGTV 540
            FRV V +  P+ +G  L + L+ARGL  T    +A+  ++  V  V +GA AV++NG V
Sbjct: 237 SFRVFVAEGAPRYQGHLLAKELVARGLQTTVITDSAVFAMISRVNMVIIGAHAVMANGGV 296

Query: 541 YSRVGTAAVAMVANAFHVPVLICCEAYKFHERVQLD-SICFNELGDPDTISKVPERKDIN 600
              VG    A+ A    VP ++   ++K       +  +  NEL  P  +    E  D  
Sbjct: 297 IGPVGVNMAALAAQKHAVPFVVLAGSHKLCPLYPHNPEVLLNELRSPSELLDFGEFSDCL 356

Query: 601 DL-AAKEQLQLLNLMYDATPSDYISMIVTDYGMIPPTSVPVIVREY 645
           D  A    LQ++N  +D  P + +S+ +TD G   P+ +  ++ +Y
Sbjct: 357 DFGAGSGSLQVVNPTFDYVPPNLVSLFITDTGGHNPSYMYRLIADY 401

BLAST of Cucsa.198810 vs. TAIR10
Match: AT1G72340.1 (AT1G72340.1 NagB/RpiA/CoA transferase-like superfamily protein)

HSP 1 Score: 80.1 bits (196), Expect = 5.6e-15
Identity = 56/198 (28.28%), Postives = 93/198 (46.97%), Query Frame = 1

Query: 441 ADEVIVRHAATKIRDGDVLLTYGSSSAVEMLILYAHELGKEFRVVVVDSRPKQEGKALLR 500
           A  +I   +   I DG  +L +G S  V  ++  A +  K FRV+  + RP   G  L  
Sbjct: 187 ARRIIAMLSQDFIFDGCTILVHGLSRVVLEILKTAAQNNKLFRVLCTEGRPDGTGVLLSS 246

Query: 501 RLIARGLNCTYTHINAISYVMHEVTRVFLGAAAVLSNGTVYSRVGTAAVAMVANAFHVPV 560
            L    +       +A++Y M EV  VF+GA  V+ +G + + +GT  +A+VA++ + PV
Sbjct: 247 ELSKLDIPVKLLLDSAVAYSMDEVDMVFVGADGVVESGGIINMMGTYQIALVAHSMNKPV 306

Query: 561 LICCEAYKFHERVQLDSICFNELGDPDTIS-KVPERKDINDLAAKEQLQLLNLMYDATPS 620
            +  E+YKF     LD         P     K+P + ++   A            D TP 
Sbjct: 307 YVAAESYKFARLYPLDQKDMAPALRPIEFGVKIPTKVEVERSAR-----------DYTPP 366

Query: 621 DYISMIVTDYGMIPPTSV 638
            Y++++ TD G++ P+ V
Sbjct: 367 QYLTLLFTDLGVLSPSVV 373

BLAST of Cucsa.198810 vs. NCBI nr
Match: gi|778656271|ref|XP_004138224.2| (PREDICTED: translation initiation factor eIF-2B subunit delta-like isoform X1 [Cucumis sativus])

HSP 1 Score: 1291.9 bits (3342), Expect = 0.0e+00
Identity = 650/651 (99.85%), Postives = 651/651 (100.00%), Query Frame = 1

Query: 1   MDARRGSRIVNPKVRQVGFFTLDAPNSPDRSLSGPPNCLPSQQSLSPPTNSLSPVMIPPP 60
           MDARRGSRIVNPKVRQVGFFTLDAPNSPDRSLSGPPNCLPSQQSLSPPTNSLSPVMIPPP
Sbjct: 1   MDARRGSRIVNPKVRQVGFFTLDAPNSPDRSLSGPPNCLPSQQSLSPPTNSLSPVMIPPP 60

Query: 61  RHLSDNLASRSAVLPVPAPANWRNSTDQIPVGSYNPSETLLATSPMASPSSRTGDEELDM 120
           RHLSDNLASRSAVLPVPAPANWRNSTDQIPVGSYNPSETLLATSPMASPSSRTGDEELDM
Sbjct: 61  RHLSDNLASRSAVLPVPAPANWRNSTDQIPVGSYNPSETLLATSPMASPSSRTGDEELDM 120

Query: 121 SEDSSNSVWHRRSGSGKIASSFPGVGIHVGAKSLENSQAVPLQTLETNKPAGVGEKTGGP 180
           SEDSSNSVWHRRSGSGKIASSFPGVGIHVGAKSLENSQAVPLQTLETNKPAGVGEKTGGP
Sbjct: 121 SEDSSNSVWHRRSGSGKIASSFPGVGIHVGAKSLENSQAVPLQTLETNKPAGVGEKTGGP 180

Query: 181 STEVQNRQPPPNSKPLKAKTTKAERRALQEAQRAAKVSAKGEANKSGAAPGKAVKQPAAM 240
           STEVQNRQPPPNSKPLKAKTTKAERRALQEAQRAAKVSAKGEANKSGAAPGKAVKQPAAM
Sbjct: 181 STEVQNRQPPPNSKPLKAKTTKAERRALQEAQRAAKVSAKGEANKSGAAPGKAVKQPAAM 240

Query: 241 KDSTAAPSVAPTDKRGGGDRTLDKERKKDAPPPRMQFDDKSRVEKAKKRAVFNQTEARNR 300
           KDSTAAPSVAPTDKRGGGDRTLDKERKKDAPPPRMQFDDKSRVEKAKKRAVFNQTEARNR
Sbjct: 241 KDSTAAPSVAPTDKRGGGDRTLDKERKKDAPPPRMQFDDKSRVEKAKKRAVFNQTEARNR 300

Query: 301 VELFRHLPQYEQGTQLPDLVSKFFQLDSIHPAIYKVGLQYMARDICGGNARCIAMLHAFQ 360
           VELFRHLPQYEQGTQLPDLVSKFFQLDSIHPAIYKVGLQYMARDICGGNARCIAMLHAFQ
Sbjct: 301 VELFRHLPQYEQGTQLPDLVSKFFQLDSIHPAIYKVGLQYMARDICGGNARCIAMLHAFQ 360

Query: 361 QAIKDYSTPPQKSLGRDLTAKIGSYVSFFNECRPLSVSMGNAIRFLKNRIAKLPLNLPES 420
           Q+IKDYSTPPQKSLGRDLTAKIGSYVSFFNECRPLSVSMGNAIRFLKNRIAKLPLNLPES
Sbjct: 361 QSIKDYSTPPQKSLGRDLTAKIGSYVSFFNECRPLSVSMGNAIRFLKNRIAKLPLNLPES 420

Query: 421 EVKATLCSDINRFINEKIIIADEVIVRHAATKIRDGDVLLTYGSSSAVEMLILYAHELGK 480
           EVKATLCSDINRFINEKIIIADEVIVRHAATKIRDGDVLLTYGSSSAVEMLILYAHELGK
Sbjct: 421 EVKATLCSDINRFINEKIIIADEVIVRHAATKIRDGDVLLTYGSSSAVEMLILYAHELGK 480

Query: 481 EFRVVVVDSRPKQEGKALLRRLIARGLNCTYTHINAISYVMHEVTRVFLGAAAVLSNGTV 540
           EFRVVVVDSRPKQEGKALLRRLIARGLNCTYTHINAISYVMHEVTRVFLGAAAVLSNGTV
Sbjct: 481 EFRVVVVDSRPKQEGKALLRRLIARGLNCTYTHINAISYVMHEVTRVFLGAAAVLSNGTV 540

Query: 541 YSRVGTAAVAMVANAFHVPVLICCEAYKFHERVQLDSICFNELGDPDTISKVPERKDIND 600
           YSRVGTAAVAMVANAFHVPVLICCEAYKFHERVQLDSICFNELGDPDTISKVPERKDIND
Sbjct: 541 YSRVGTAAVAMVANAFHVPVLICCEAYKFHERVQLDSICFNELGDPDTISKVPERKDIND 600

Query: 601 LAAKEQLQLLNLMYDATPSDYISMIVTDYGMIPPTSVPVIVREYRREHLWI 652
           LAAKEQLQLLNLMYDATPSDYISMIVTDYGMIPPTSVPVIVREYRREHLWI
Sbjct: 601 LAAKEQLQLLNLMYDATPSDYISMIVTDYGMIPPTSVPVIVREYRREHLWI 651

BLAST of Cucsa.198810 vs. NCBI nr
Match: gi|659106513|ref|XP_008453361.1| (PREDICTED: translation initiation factor eIF-2B subunit delta-like [Cucumis melo])

HSP 1 Score: 1269.2 bits (3283), Expect = 0.0e+00
Identity = 645/655 (98.47%), Postives = 649/655 (99.08%), Query Frame = 1

Query: 1   MDARRGSRIVNPKVRQVGFFTLDAPNSPDRSLSGPPNCLPSQQSLSPPTNSLSPVMIPPP 60
           MDARRGSRIVNPKVRQVGFFTLDAPNSPDRSLSGPPNCLPSQQSLSPPTNSLSPVMIPPP
Sbjct: 1   MDARRGSRIVNPKVRQVGFFTLDAPNSPDRSLSGPPNCLPSQQSLSPPTNSLSPVMIPPP 60

Query: 61  RHLSDNLASRSAVLPVPAPANWRNSTDQIPVGSYNPSETLLATSPMASPSSRTGDEELDM 120
           RHLSDNLASRSAVLPVPAPANWRNSTDQIPVGSYNPSETLLATSPMASPSSRTGDEELDM
Sbjct: 61  RHLSDNLASRSAVLPVPAPANWRNSTDQIPVGSYNPSETLLATSPMASPSSRTGDEELDM 120

Query: 121 SEDSSNSVWHRRSGSGKIASSFPGVGIHVGAKSLENSQAV-PLQTLETNKPAGVGEKTGG 180
           SEDSSNSVWHRRSGSGKIASSFPGVGIH+GAKSLENSQAV PLQTLETNKPAGVGEKTGG
Sbjct: 121 SEDSSNSVWHRRSGSGKIASSFPGVGIHMGAKSLENSQAVVPLQTLETNKPAGVGEKTGG 180

Query: 181 PSTEVQNRQPPPNSKPLKAKTTKAERRALQEAQRAAKVSAKGEANKSGAAPGKAVKQPAA 240
           PSTEVQNRQPPPNSKPLKAKTTKAERRALQEAQRAAKVSAKGEANKSGAA GKAVKQPAA
Sbjct: 181 PSTEVQNRQPPPNSKPLKAKTTKAERRALQEAQRAAKVSAKGEANKSGAASGKAVKQPAA 240

Query: 241 MKDSTAAPSVAPTDKRGGGDRTLDKERKKDAPPPRMQFDDKSRVEKAKKRAVFNQTEARN 300
           MKDSTAAPSVAPTDKRGG DRTLDKERKKDAPPPR+QFDDKSRVEKAKKRAVFNQTEARN
Sbjct: 241 MKDSTAAPSVAPTDKRGG-DRTLDKERKKDAPPPRLQFDDKSRVEKAKKRAVFNQTEARN 300

Query: 301 RVELFRHLPQYEQGTQLPDLVSKFFQLDSIHPAIYKVGLQYMARDICGGNARCIAMLHAF 360
           RVELFRHLPQYEQGTQLPDLVSKFFQLDSIHPA+YKVGLQYMARDICGGNARCIAMLHAF
Sbjct: 301 RVELFRHLPQYEQGTQLPDLVSKFFQLDSIHPAVYKVGLQYMARDICGGNARCIAMLHAF 360

Query: 361 QQAIKDYSTPPQKSLGRDLTAKIGSYVSFFNECRPLSVSMGNAIRFLKNRIAKLPLNLPE 420
           QQAIKDYSTPPQKSLGRDLTAKIGSYVSFFNECRPLSVSMGNAIRFLKNRIAKLPLNLPE
Sbjct: 361 QQAIKDYSTPPQKSLGRDLTAKIGSYVSFFNECRPLSVSMGNAIRFLKNRIAKLPLNLPE 420

Query: 421 SEVKATLCSDINRFINEKIIIADEVIVRHAATKIRDGDVLLTYGSSSAVEMLILYAHELG 480
           SEVKATLCSDINRFINEKIIIADEVIVRHAATKIRDGDVLLTYGSSSAVEMLILYAHELG
Sbjct: 421 SEVKATLCSDINRFINEKIIIADEVIVRHAATKIRDGDVLLTYGSSSAVEMLILYAHELG 480

Query: 481 KEFRVVVVDSRPKQEGKALLRRLIARGLNCTYTHINAISYVMHEVTRVFLGAAAVLSNGT 540
           KEFRVVVVDSRPKQEGKALLRRLIARGLNCTYTHINAISY+MHEVTRVFLGAAAVLSNGT
Sbjct: 481 KEFRVVVVDSRPKQEGKALLRRLIARGLNCTYTHINAISYMMHEVTRVFLGAAAVLSNGT 540

Query: 541 VYSRVGTAAVAMVANAFHVPVLICCEAYKFHERVQLDSICFNELGDPDTISKVPERKDIN 600
           VYSRVGTAAVAMVANAFHVPVLICCEAYKFHERVQLDSICFNELGDPDTISKVPERKDIN
Sbjct: 541 VYSRVGTAAVAMVANAFHVPVLICCEAYKFHERVQLDSICFNELGDPDTISKVPERKDIN 600

Query: 601 ---DLAAKEQLQLLNLMYDATPSDYISMIVTDYGMIPPTSVPVIVREYRREHLWI 652
              DLAAKEQLQLLNLMYDATPSDYISMIVTDYGMIPPTSVPVIVREYRREHLWI
Sbjct: 601 YLDDLAAKEQLQLLNLMYDATPSDYISMIVTDYGMIPPTSVPVIVREYRREHLWI 654

BLAST of Cucsa.198810 vs. NCBI nr
Match: gi|659106503|ref|XP_008453356.1| (PREDICTED: translation initiation factor eIF-2B subunit delta-like isoform X1 [Cucumis melo])

HSP 1 Score: 1124.4 bits (2907), Expect = 0.0e+00
Identity = 577/654 (88.23%), Postives = 598/654 (91.44%), Query Frame = 1

Query: 1   MDARRGSRIVNPKVRQVGFFTLDAPNSPDRSLSGPPNCLPSQQSLSPPTNSLSPVMIPPP 60
           MDARRGSR+VNPKVRQVGFFTLDAPN P RSLSGP +C PSQQSLSPP+NSLSPVMIPPP
Sbjct: 1   MDARRGSRVVNPKVRQVGFFTLDAPNPPGRSLSGPADCTPSQQSLSPPSNSLSPVMIPPP 60

Query: 61  RHLSDNLASRSAVLPVPAPANWRNSTDQIPVGSYNPSETLLATSPMASPSSRTGDEELDM 120
           RHLSDNLASRSAV P+P PANWR+S D I VGSYNPSE LLATSP    S R GDEELDM
Sbjct: 61  RHLSDNLASRSAVFPLPPPANWRDSADHIHVGSYNPSEMLLATSP----SGRIGDEELDM 120

Query: 121 SEDSSNSVWHRRSGSGKIASSFPGVGIHVGAKSLENSQAVPLQTLETNKPAGVGEKTGGP 180
           SEDSS SV +RRSGSGKIASSFPGVGI++ AK+ E SQA+ + T+E NK  GV EK GG 
Sbjct: 121 SEDSSTSVPYRRSGSGKIASSFPGVGIYMAAKASETSQAMVVNTVEANKSVGVAEKNGGA 180

Query: 181 STEVQNRQPPPNSKPLKAKTTKAERRALQEAQRAAKVSAKGEANKSGAAPGKAVKQPAAM 240
           STEVQNRQPP NSKPLKAKTTKAERRALQEAQRAAK SA GE  KSG  PGKAVKQ + M
Sbjct: 181 STEVQNRQPPSNSKPLKAKTTKAERRALQEAQRAAKGSANGEVKKSGNTPGKAVKQSSTM 240

Query: 241 KDSTAAPSVAPTDKRGGGDRTLDKERKKDAPPPRMQFDDKSRVEKAKKRAVFNQTEARNR 300
           +DSTAAPSVA TDKRGG DRT DKERKKDAPPPRMQFDDKSRVEKAKKRAVFNQTEARNR
Sbjct: 241 RDSTAAPSVASTDKRGG-DRTHDKERKKDAPPPRMQFDDKSRVEKAKKRAVFNQTEARNR 300

Query: 301 VELFRHLPQYEQGTQLPDLVSKFFQLDSIHPAIYKVGLQYMARDICGGNARCIAMLHAFQ 360
           VELFRHLPQYEQGTQLPDLVSKFFQLD IHPAIYKVGLQYMA DICGGNARCIAMLHAFQ
Sbjct: 301 VELFRHLPQYEQGTQLPDLVSKFFQLDPIHPAIYKVGLQYMAGDICGGNARCIAMLHAFQ 360

Query: 361 QAIKDYSTPPQKSLGRDLTAKIGSYVSFFNECRPLSVSMGNAIRFLKNRIAKLPLNLPES 420
           QAIKDYSTPPQKSLGRDLT KIG YVSFFNECRPLSVSMGNAIRFLKN IAKLPLNLPES
Sbjct: 361 QAIKDYSTPPQKSLGRDLTTKIGGYVSFFNECRPLSVSMGNAIRFLKNLIAKLPLNLPES 420

Query: 421 EVKATLCSDINRFINEKIIIADEVIVRHAATKIRDGDVLLTYGSSSAVEMLILYAHELGK 480
           EVKATLCSDI+RFINEKIIIADEVIVRHAATKIRDGDVLLTYGSS +VEML+LYAHELGK
Sbjct: 421 EVKATLCSDIDRFINEKIIIADEVIVRHAATKIRDGDVLLTYGSSCSVEMLLLYAHELGK 480

Query: 481 EFRVVVVDSRPKQEGKALLRRLIARGLNCTYTHINAISYVMHEVTRVFLGAAAVLSNGTV 540
           EFRVVVVDSRPK EG+ALLRRLI RGL+CTYTHINAISYVMH+VTRVFLGAAAVLSNGTV
Sbjct: 481 EFRVVVVDSRPKLEGQALLRRLIGRGLSCTYTHINAISYVMHKVTRVFLGAAAVLSNGTV 540

Query: 541 YSRVGTAAVAMVANAFHVPVLICCEAYKFHERVQLDSICFNELGDPDTISKVPERKDIN- 600
           YSRVGTAAVAMVANAFHVPVLICCEAYKFHERVQLDSICFNELGDPD ISKV  RKDIN 
Sbjct: 541 YSRVGTAAVAMVANAFHVPVLICCEAYKFHERVQLDSICFNELGDPDAISKVQGRKDINH 600

Query: 601 --DLAAKEQLQLLNLMYDATPSDYISMIVTDYGMIPPTSVPVIVREYRREHLWI 652
             +LAAKEQLQ LNLMYDATPSDYISMIVTDYGMIPPTSVPVIVREYRREHLWI
Sbjct: 601 LENLAAKEQLQFLNLMYDATPSDYISMIVTDYGMIPPTSVPVIVREYRREHLWI 649

BLAST of Cucsa.198810 vs. NCBI nr
Match: gi|659106505|ref|XP_008453358.1| (PREDICTED: translation initiation factor eIF-2B subunit delta-like isoform X2 [Cucumis melo])

HSP 1 Score: 1116.7 bits (2887), Expect = 0.0e+00
Identity = 575/654 (87.92%), Postives = 596/654 (91.13%), Query Frame = 1

Query: 1   MDARRGSRIVNPKVRQVGFFTLDAPNSPDRSLSGPPNCLPSQQSLSPPTNSLSPVMIPPP 60
           MDARRGSR+VNPKVRQVGFFTLDAPN P RSLSGP +C PSQQSLSPP+NSLSPVMIPPP
Sbjct: 1   MDARRGSRVVNPKVRQVGFFTLDAPNPPGRSLSGPADCTPSQQSLSPPSNSLSPVMIPPP 60

Query: 61  RHLSDNLASRSAVLPVPAPANWRNSTDQIPVGSYNPSETLLATSPMASPSSRTGDEELDM 120
           RHLSDNLASRSAV P+P PANWR+S D I VGSYNPSE LLATSP    S R GDEELDM
Sbjct: 61  RHLSDNLASRSAVFPLPPPANWRDSADHIHVGSYNPSEMLLATSP----SGRIGDEELDM 120

Query: 121 SEDSSNSVWHRRSGSGKIASSFPGVGIHVGAKSLENSQAVPLQTLETNKPAGVGEKTGGP 180
           SEDSS SV +RRSGSGKIASSFPGVGI++ AK+ E SQA+ + T+E NK  GV EK GG 
Sbjct: 121 SEDSSTSVPYRRSGSGKIASSFPGVGIYMAAKASETSQAMVVNTVEANKSVGVAEKNGGA 180

Query: 181 STEVQNRQPPPNSKPLKAKTTKAERRALQEAQRAAKVSAKGEANKSGAAPGKAVKQPAAM 240
           STEVQNRQPP NSKPLKAKTTKAERRALQEAQRAAK    GE  KSG  PGKAVKQ + M
Sbjct: 181 STEVQNRQPPSNSKPLKAKTTKAERRALQEAQRAAK----GEVKKSGNTPGKAVKQSSTM 240

Query: 241 KDSTAAPSVAPTDKRGGGDRTLDKERKKDAPPPRMQFDDKSRVEKAKKRAVFNQTEARNR 300
           +DSTAAPSVA TDKRGG DRT DKERKKDAPPPRMQFDDKSRVEKAKKRAVFNQTEARNR
Sbjct: 241 RDSTAAPSVASTDKRGG-DRTHDKERKKDAPPPRMQFDDKSRVEKAKKRAVFNQTEARNR 300

Query: 301 VELFRHLPQYEQGTQLPDLVSKFFQLDSIHPAIYKVGLQYMARDICGGNARCIAMLHAFQ 360
           VELFRHLPQYEQGTQLPDLVSKFFQLD IHPAIYKVGLQYMA DICGGNARCIAMLHAFQ
Sbjct: 301 VELFRHLPQYEQGTQLPDLVSKFFQLDPIHPAIYKVGLQYMAGDICGGNARCIAMLHAFQ 360

Query: 361 QAIKDYSTPPQKSLGRDLTAKIGSYVSFFNECRPLSVSMGNAIRFLKNRIAKLPLNLPES 420
           QAIKDYSTPPQKSLGRDLT KIG YVSFFNECRPLSVSMGNAIRFLKN IAKLPLNLPES
Sbjct: 361 QAIKDYSTPPQKSLGRDLTTKIGGYVSFFNECRPLSVSMGNAIRFLKNLIAKLPLNLPES 420

Query: 421 EVKATLCSDINRFINEKIIIADEVIVRHAATKIRDGDVLLTYGSSSAVEMLILYAHELGK 480
           EVKATLCSDI+RFINEKIIIADEVIVRHAATKIRDGDVLLTYGSS +VEML+LYAHELGK
Sbjct: 421 EVKATLCSDIDRFINEKIIIADEVIVRHAATKIRDGDVLLTYGSSCSVEMLLLYAHELGK 480

Query: 481 EFRVVVVDSRPKQEGKALLRRLIARGLNCTYTHINAISYVMHEVTRVFLGAAAVLSNGTV 540
           EFRVVVVDSRPK EG+ALLRRLI RGL+CTYTHINAISYVMH+VTRVFLGAAAVLSNGTV
Sbjct: 481 EFRVVVVDSRPKLEGQALLRRLIGRGLSCTYTHINAISYVMHKVTRVFLGAAAVLSNGTV 540

Query: 541 YSRVGTAAVAMVANAFHVPVLICCEAYKFHERVQLDSICFNELGDPDTISKVPERKDIN- 600
           YSRVGTAAVAMVANAFHVPVLICCEAYKFHERVQLDSICFNELGDPD ISKV  RKDIN 
Sbjct: 541 YSRVGTAAVAMVANAFHVPVLICCEAYKFHERVQLDSICFNELGDPDAISKVQGRKDINH 600

Query: 601 --DLAAKEQLQLLNLMYDATPSDYISMIVTDYGMIPPTSVPVIVREYRREHLWI 652
             +LAAKEQLQ LNLMYDATPSDYISMIVTDYGMIPPTSVPVIVREYRREHLWI
Sbjct: 601 LENLAAKEQLQFLNLMYDATPSDYISMIVTDYGMIPPTSVPVIVREYRREHLWI 645

BLAST of Cucsa.198810 vs. NCBI nr
Match: gi|778656268|ref|XP_011648725.1| (PREDICTED: translation initiation factor eIF-2B subunit delta-like [Cucumis sativus])

HSP 1 Score: 1112.4 bits (2876), Expect = 0.0e+00
Identity = 573/654 (87.61%), Postives = 595/654 (90.98%), Query Frame = 1

Query: 1   MDARRGSRIVNPKVRQVGFFTLDAPNSPDRSLSGPPNCLPSQQSLSPPTNSLSPVMIPPP 60
           MDARRGSR+VNPK+RQVGFFTLD PN P RSLSGP +C PSQQSLSPP+NSLSPVMIPPP
Sbjct: 1   MDARRGSRVVNPKIRQVGFFTLDPPNPPGRSLSGPADCTPSQQSLSPPSNSLSPVMIPPP 60

Query: 61  RHLSDNLASRSAVLPVPAPANWRNSTDQIPVGSYNPSETLLATSPMASPSSRTGDEELDM 120
           RHLSDNLASRSAV P+P PANWR+S D I VGSYNPSE LLATSP    S R GDEELDM
Sbjct: 61  RHLSDNLASRSAVFPLPPPANWRDSADHIHVGSYNPSEMLLATSP----SGRIGDEELDM 120

Query: 121 SEDSSNSVWHRRSGSGKIASSFPGVGIHVGAKSLENSQAVPLQTLETNKPAGVGEKTGGP 180
           SEDS  SV +RRSGSGKIASSFPGVGI++ AK+ E SQA+   T+E NK  GV EK GG 
Sbjct: 121 SEDSFTSVPYRRSGSGKIASSFPGVGIYMAAKASETSQAMVPNTVEANKSVGVAEKNGGA 180

Query: 181 STEVQNRQPPPNSKPLKAKTTKAERRALQEAQRAAKVSAKGEANKSGAAPGKAVKQPAAM 240
           STE+QNRQPP NSKP KAKTTKAERRALQEAQRAAK    GEA KS  APGKAVKQ + M
Sbjct: 181 STELQNRQPPSNSKPQKAKTTKAERRALQEAQRAAK----GEAKKSSNAPGKAVKQSSTM 240

Query: 241 KDSTAAPSVAPTDKRGGGDRTLDKERKKDAPPPRMQFDDKSRVEKAKKRAVFNQTEARNR 300
           +DSTAAPSVA TDKRGG DRT DKERKKDAPPPRMQFDDKSRVEKAKKRAVFNQTEARNR
Sbjct: 241 RDSTAAPSVASTDKRGG-DRTHDKERKKDAPPPRMQFDDKSRVEKAKKRAVFNQTEARNR 300

Query: 301 VELFRHLPQYEQGTQLPDLVSKFFQLDSIHPAIYKVGLQYMARDICGGNARCIAMLHAFQ 360
           VELFRHLPQYEQGTQLPDLVSKFFQLD IHPAIYKVGLQYMA DICGGNARCIAMLHAFQ
Sbjct: 301 VELFRHLPQYEQGTQLPDLVSKFFQLDPIHPAIYKVGLQYMAGDICGGNARCIAMLHAFQ 360

Query: 361 QAIKDYSTPPQKSLGRDLTAKIGSYVSFFNECRPLSVSMGNAIRFLKNRIAKLPLNLPES 420
           QAIKDYSTPPQKSLGRDLT KIG YVSFFNECRPLSVSMGNAIRFLKN IAKLPLNLPES
Sbjct: 361 QAIKDYSTPPQKSLGRDLTTKIGGYVSFFNECRPLSVSMGNAIRFLKNLIAKLPLNLPES 420

Query: 421 EVKATLCSDINRFINEKIIIADEVIVRHAATKIRDGDVLLTYGSSSAVEMLILYAHELGK 480
           EVKATLCSDI+RFINEKIIIADEVIVRHAATKIRDGDVLLTYGSS AVEML+LYAHELGK
Sbjct: 421 EVKATLCSDIDRFINEKIIIADEVIVRHAATKIRDGDVLLTYGSSCAVEMLLLYAHELGK 480

Query: 481 EFRVVVVDSRPKQEGKALLRRLIARGLNCTYTHINAISYVMHEVTRVFLGAAAVLSNGTV 540
           EFRVVVVDSRPK EG+ALLRRLI RGL+CTYTHINAISY+MH+VTRVFLGAAAVLSNGTV
Sbjct: 481 EFRVVVVDSRPKLEGQALLRRLIGRGLSCTYTHINAISYMMHKVTRVFLGAAAVLSNGTV 540

Query: 541 YSRVGTAAVAMVANAFHVPVLICCEAYKFHERVQLDSICFNELGDPDTISKVPERKDIN- 600
           YSRVGTAAVAMVANAFHVPVLICCEAYKFHERVQLDSICFNELGDPD ISKVP RKDIN 
Sbjct: 541 YSRVGTAAVAMVANAFHVPVLICCEAYKFHERVQLDSICFNELGDPDAISKVPGRKDINH 600

Query: 601 --DLAAKEQLQLLNLMYDATPSDYISMIVTDYGMIPPTSVPVIVREYRREHLWI 652
             +LAAKEQLQLLNLMYDATPSDYISMIVTDYGMIPPTSVPVIVREYRREHLWI
Sbjct: 601 LENLAAKEQLQLLNLMYDATPSDYISMIVTDYGMIPPTSVPVIVREYRREHLWI 645