Cucsa.198800 (gene) Cucumber (Gy14) v1

BLAST of Cucsa.198800 vs. Swiss-Prot
Match: EI2BD_RABIT (Translation initiation factor eIF-2B subunit delta OS=Oryctolagus cuniculus GN=EIF2B4 PE=2 SV=2)

HSP 1 Score: 329.7 bits (844), Expect = 7.1e-89
Identity = 195/479 (40.71%), Postives = 273/479 (56.99%), Query Frame = 1

Query: 148 KASETSQAMVPNTVEANKSVGVAEKNGGASTELQNRQPPSNSKPQKAKTTKAERRALQEA 207
           K ++   A   +  +       A   G  S+    + P   +K +     +AERRA QEA
Sbjct: 54  KGTDVDTASAVSAAQCPGPAREAPGPGSQSSTPGEKVPAGRTKAE----LRAERRAKQEA 113

Query: 208 QRAAKGEAKKSSNAPGKAVKQSSTMRDSTAAPSVASTDKRGGDRTHDKERKKDAPPPRMQ 267
           +RA K   K     P              A+PS A     GG R  +  +  D    R  
Sbjct: 114 ERALKQARKGEQGGP-----------PPQASPSTAGEAPAGGKRLTEHTQADDPTLLRRL 173

Query: 268 FDDKSRVEKAKKRAVFNQTEARNRVELFRHLPQYEQGTQLPDLVSKFFQLDPIHPAIYKV 327
                 V K++++ V  + +  ++V LF HLPQY +   L   +S       IHPA+ ++
Sbjct: 174 ------VRKSERQQVPTRKDYGSKVSLFSHLPQYSRQNSLTQYMS--IPSSVIHPAMVRL 233

Query: 328 GLQYMAGDICGGNARCIAMLHAFQQAIKDYSTPPQKSLGRDLTTKIGGYVSFFNECRPLS 387
           GLQY  G I G NARCIA+L A QQ I+DY+TPP + L RDL  K+  Y+ F  +CRPLS
Sbjct: 234 GLQYSQGLISGSNARCIALLRALQQVIQDYTTPPNEELSRDLVNKLKPYICFLTQCRPLS 293

Query: 388 VSMGNAIRFLKNLIAKLPLNLPESEVKATLCSDIDRFINEKIIIADEVIVRHAATKIRDG 447
            SM NAI+FL   I  +     E E KA L +  DR++ EKI++A + I+R A+ KI +G
Sbjct: 294 ASMYNAIKFLNKEITGVSSTKREEEAKAELQAAADRYVQEKIVLAAQAILRFASKKISNG 353

Query: 448 DVLLTYGSSCAVEMLLLYAHELGKEFRVVVVDSRPKLEGQALLRRLIGRGLSCTYTHINA 507
           DV+L YG S  V  +L  A   G++FRVVVVDSRP+LEG+ +LR L+  G+  +Y  I A
Sbjct: 354 DVILVYGCSSLVSRILQEAWSEGRKFRVVVVDSRPRLEGRHMLRFLVRAGVPASYLLIPA 413

Query: 508 ISYMMHKVTRVFLGAAAVLSNGTVYSRVGTAAVAMVANAFHVPVLICCEAYKFHERVQLD 567
            SY++ +V++V LGA A+L+NG+V SRVGTA +A+VA A +VPVL+CCE YKF ERVQ D
Sbjct: 414 ASYVLPEVSKVLLGAHALLANGSVMSRVGTAQLALVARAHNVPVLVCCETYKFCERVQTD 473

Query: 568 SICFNELGDPDAISKVPGRKDINHLENLAAKEQLQLLNLMYDATPSDYISMIVTDYGMV 627
           +   NEL DPD +    G  D   L N  +   L+LLNL+YD TP + + +++T+ GM+
Sbjct: 474 AFVSNELDDPDDLQCERG--DHVALANWQSHPSLRLLNLVYDVTPPELVDLVITELGMI 507

BLAST of Cucsa.198800 vs. Swiss-Prot
Match: EI2BD_RAT (Translation initiation factor eIF-2B subunit delta OS=Rattus norvegicus GN=Eif2b4 PE=2 SV=1)

HSP 1 Score: 328.2 bits (840), Expect = 2.1e-88
Identity = 193/463 (41.68%), Postives = 264/463 (57.02%), Query Frame = 1

Query: 168 GVAEKNGGASTELQNRQPPSNSKPQKAKTTKAERRALQEAQRAAKGEAKKSSNAPGKAVK 227
           G   + GG + E   + P   SK +     +AERRA QEA+RA K   K     P     
Sbjct: 78  GTGSQLGGTTGE---KLPAGRSKAE----LRAERRAKQEAERALKQARKGEQGGPSPQ-- 137

Query: 228 QSSTMRDSTAAPSVASTDKRGGDRTHDKERKKDAPPPRMQFDDKSRVEKAKKRAVFNQTE 287
                    A PS A     G  R           P   Q DD + + +  ++    Q  
Sbjct: 138 ---------ACPSTAGEATSGVKRV----------PEHTQADDPTLLRRLLRKPDRQQVP 197

Query: 288 AR----NRVELFRHLPQYEQGTQLPDLVSKFFQLDPIHPAIYKVGLQYMAGDICGGNARC 347
            R    ++V LF HLPQY + + L   +S       IHPA+ ++GLQY  G + G NARC
Sbjct: 198 TRKDYGSKVSLFSHLPQYSRQSSLTQYMS--IPSSVIHPAMVRLGLQYSQGLVSGSNARC 257

Query: 348 IAMLHAFQQAIKDYSTPPQKSLGRDLTTKIGGYVSFFNECRPLSVSMGNAIRFLKNLIAK 407
           IA+LHA QQ I+DY+TPP + L RDL  K+  Y+SF  +CRP+S SM NAI+F    +  
Sbjct: 258 IALLHALQQVIQDYTTPPNEELSRDLVNKLKPYISFLTQCRPMSASMCNAIKFFNKEVTG 317

Query: 408 LPLNLPESEVKATLCSDIDRFINEKIIIADEVIVRHAATKIRDGDVLLTYGSSCAVEMLL 467
           +  +  E E K+ L   IDR++ EKI++A + I R A+ KI DGDV+L YG S  V  +L
Sbjct: 318 MSSSKREEEAKSELKEAIDRYVQEKIVLASQAISRFASKKISDGDVILVYGCSSLVSRIL 377

Query: 468 LYAHELGKEFRVVVVDSRPKLEGQALLRRLIGRGLSCTYTHINAISYMMHKVTRVFLGAA 527
             A   G+ FRVVVVDSRP+LEG+ +L  L+  G+  +Y  I A SY++ +V++V LGA 
Sbjct: 378 QEAWVEGRRFRVVVVDSRPRLEGRHMLHCLVRAGVPTSYLLIPAASYVLPEVSKVLLGAH 437

Query: 528 AVLSNGTVYSRVGTAAVAMVANAFHVPVLICCEAYKFHERVQLDSICFNELGDPDAISKV 587
           A+L+NG+V SRVGTA +A+VA A +VPVL+CCE YKF ERVQ D+   NEL DPD +   
Sbjct: 438 ALLANGSVMSRVGTAQLALVARAHNVPVLVCCETYKFCERVQTDAFVSNELDDPDDLQCK 497

Query: 588 PGRKDINHLENLAAKEQLQLLNLMYDATPSDYISMIVTDYGMV 627
            G  D   L N      L+LLNL+YD TP + + +++T+ GM+
Sbjct: 498 RG--DQVTLANWQNNSSLRLLNLVYDVTPPELVDLVITELGMI 508

BLAST of Cucsa.198800 vs. Swiss-Prot
Match: EI2BD_MOUSE (Translation initiation factor eIF-2B subunit delta OS=Mus musculus GN=Eif2b4 PE=1 SV=2)

HSP 1 Score: 327.4 bits (838), Expect = 3.5e-88
Identity = 195/459 (42.48%), Postives = 273/459 (59.48%), Query Frame = 1

Query: 168 GVAEKNGGASTELQNRQPPSNSKPQKAKTTKAERRALQEAQRAAKGEAKKSSNAPGKAVK 227
           G   + GG + E   + P   SK +     +AERRA QEA+RA K +A+K     G    
Sbjct: 78  GPGSQLGGTAGE---KLPAGRSKAE----LRAERRAKQEAERALK-QARKGEQ--GGVPP 137

Query: 228 QSSTMRDSTAAPSVASTDKRGGDRTHDKERKKDAPPPRMQFDDKSRVEKAKKRAVFNQTE 287
           Q        A PS A      G+ T   +R  +  P       +  + K  ++ V  + +
Sbjct: 138 Q--------ACPSTA------GETTSGVKRVPEHTPADDPTLLRRLLRKPDRQQVPTRKD 197

Query: 288 ARNRVELFRHLPQYEQGTQLPDLVSKFFQLDPIHPAIYKVGLQYMAGDICGGNARCIAML 347
             ++V LF HLPQY + + L   +S       IHPA+ ++GLQY  G I G NARCIA+L
Sbjct: 198 YGSKVSLFSHLPQYSRQSSLTQYMS--IPSSVIHPAMVRLGLQYSQGLISGSNARCIALL 257

Query: 348 HAFQQAIKDYSTPPQKSLGRDLTTKIGGYVSFFNECRPLSVSMGNAIRFLKNLIAKLPLN 407
           HA QQ I+DY+TPP + L RDL  K+  Y+SF  +CRP+S SM NAI+FL   +  +  +
Sbjct: 258 HALQQVIQDYTTPPSEELSRDLVNKLKPYISFLTQCRPMSASMCNAIKFLTKEVTGMSSS 317

Query: 408 LPESEVKATLCSDIDRFINEKIIIADEVIVRHAATKIRDGDVLLTYGSSCAVEMLLLYAH 467
             E E K+ L   +DR++ EKI++A + I R A+TKI DGDV+L YG S  V  +L  A 
Sbjct: 318 KREEEAKSELREALDRYVQEKIVLAAQAISRFASTKISDGDVILVYGCSSLVSRILQEAR 377

Query: 468 ELGKEFRVVVVDSRPKLEGQALLRRLIGRGLSCTYTHINAISYMMHKVTRVFLGAAAVLS 527
             G+ FRVVVVDSRP+LEG+ +L  L+  G+  +Y  I A SY++ +V++V LGA A+L+
Sbjct: 378 VEGRRFRVVVVDSRPRLEGRHMLHSLVRAGVPTSYLLIPAASYVLPEVSKVLLGAHALLA 437

Query: 528 NGTVYSRVGTAAVAMVANAFHVPVLICCEAYKFHERVQLDSICFNELGDPDAISKVPGRK 587
           NG+V SRVGTA +A+VA A +VPVL+CCE YKF ERVQ D+   NEL DPD +    G  
Sbjct: 438 NGSVMSRVGTAQLALVARAHNVPVLVCCETYKFCERVQTDAFVSNELDDPDDLQCKRG-- 497

Query: 588 DINHLENLAAKEQLQLLNLMYDATPSDYISMIVTDYGMV 627
           D   L N  +   L+LLNL+YD TP + + +++T+ GM+
Sbjct: 498 DQVALANWQSHPSLRLLNLVYDVTPPELVDLVITELGMI 508

BLAST of Cucsa.198800 vs. Swiss-Prot
Match: EI2BD_HUMAN (Translation initiation factor eIF-2B subunit delta OS=Homo sapiens GN=EIF2B4 PE=1 SV=2)

HSP 1 Score: 323.2 bits (827), Expect = 6.6e-87
Identity = 197/478 (41.21%), Postives = 269/478 (56.28%), Query Frame = 1

Query: 162 EANKSVGVAEKNGGASTEL----------QNRQPPSNSKPQKAKTTKAERRALQEAQRAA 221
           E   +V  A+   G + EL          + + P   SK +     +AERRA QEA+RA 
Sbjct: 59  ETGSAVSAAQCQVGPTRELPESGIQLGTPREKVPAGRSKAE----LRAERRAKQEAERAL 118

Query: 222 KGEAKKSSNAPGKAVKQSSTMRDSTAAPSVASTDKRGGDRTHDKERKKDAPPPRMQFDD- 281
           K   K     P              A+PS A     G  R           P   Q DD 
Sbjct: 119 KQARKGEQGGP-----------PPKASPSTAGETPSGVKRL----------PEYPQVDDL 178

Query: 282 --KSRVEKAKKRAVFNQTEARNRVELFRHLPQYEQGTQLPDLVSKFFQLDPIHPAIYKVG 341
             +  V+K +++ V  + +  ++V LF HLPQY +   L   +S       IHPA+ ++G
Sbjct: 179 LLRRLVKKPERQQVPTRKDYGSKVSLFSHLPQYSRQNSLTQFMS--IPSSVIHPAMVRLG 238

Query: 342 LQYMAGDICGGNARCIAMLHAFQQAIKDYSTPPQKSLGRDLTTKIGGYVSFFNECRPLSV 401
           LQY  G + G NARCIA+L A QQ I+DY+TPP + L RDL  K+  Y+SF  +CRPLS 
Sbjct: 239 LQYSQGLVSGSNARCIALLRALQQVIQDYTTPPNEELSRDLVNKLKPYMSFLTQCRPLSA 298

Query: 402 SMGNAIRFLKNLIAKLPLNLPESEVKATLCSDIDRFINEKIIIADEVIVRHAATKIRDGD 461
           SM NAI+FL   I  +  +  E E K+ L + IDR++ EKI++A + I R A  KI +GD
Sbjct: 299 SMHNAIKFLNKEITSVGSSKREEEAKSELRAAIDRYVQEKIVLAAQAISRFAYQKISNGD 358

Query: 462 VLLTYGSSCAVEMLLLYAHELGKEFRVVVVDSRPKLEGQALLRRLIGRGLSCTYTHINAI 521
           V+L YG S  V  +L  A   G+ FRVVVVDSRP LEG+  LR L+  G+  +Y  I A 
Sbjct: 359 VILVYGCSSLVSRILQEAWTEGRRFRVVVVDSRPWLEGRHTLRSLVHAGVPASYLLIPAA 418

Query: 522 SYMMHKVTRVFLGAAAVLSNGTVYSRVGTAAVAMVANAFHVPVLICCEAYKFHERVQLDS 581
           SY++ +V++V LGA A+L+NG+V SRVGTA +A+VA A +VPVL+CCE YKF ERVQ D+
Sbjct: 419 SYVLPEVSKVLLGAHALLANGSVMSRVGTAQLALVARAHNVPVLVCCETYKFCERVQTDA 478

Query: 582 ICFNELGDPDAISKVPGRKDINHLENLAAKEQLQLLNLMYDATPSDYISMIVTDYGMV 627
              NEL DPD +    G      L N      L+LLNL+YD TP + + +++T+ GM+
Sbjct: 479 FVSNELDDPDDLQCKRGEHVA--LANWQNHASLRLLNLVYDVTPPELVDLVITELGMI 507

BLAST of Cucsa.198800 vs. Swiss-Prot
Match: EI2BD_BOVIN (Translation initiation factor eIF-2B subunit delta OS=Bos taurus GN=EIF2B4 PE=2 SV=1)

HSP 1 Score: 322.0 bits (824), Expect = 1.5e-86
Identity = 198/486 (40.74%), Postives = 274/486 (56.38%), Query Frame = 1

Query: 148 KASETSQAMVPNTVEANKSVGVAEKNGGASTEL---QNRQPPSNSKPQKAKTTKAERRAL 207
           K +ET      +  +    VG A+   G  ++L   + + P   SK +     +AERRA 
Sbjct: 54  KGTETETGSAVSAAQCQ--VGPAKHLAGPDSQLGTAKEKVPAGRSKAE----LRAERRAK 113

Query: 208 QEAQRAAKGEAKKSSNAPGKAVKQSSTMRDSTAAPSVASTDKRGGDRTHDKERKKDAPPP 267
           QEA+RA K   K     P              A PS A     G  R           P 
Sbjct: 114 QEAERAMKQARKGDQGGP-----------PPQACPSTAGETPSGVKRL----------PE 173

Query: 268 RMQFDD----KSRVEKAKKRAVFNQTEARNRVELFRHLPQYEQGTQLPDLVSKFFQLDPI 327
             Q DD    +  V+K +++ V  + +  ++V LF HLPQY +   L   +S       I
Sbjct: 174 HSQVDDPTLLRRLVKKPERQQVPTRKDYGSKVSLFSHLPQYSRQNSLTQFMS--IPSSVI 233

Query: 328 HPAIYKVGLQYMAGDICGGNARCIAMLHAFQQAIKDYSTPPQKSLGRDLTTKIGGYVSFF 387
           HPA+ ++GLQY  G + G NARCIA+L A QQ I+DY+TPP + L RDL  K+  Y SF 
Sbjct: 234 HPAMVRLGLQYSQGLVSGSNARCIALLRALQQVIQDYTTPPSEELSRDLVNKLKPYFSFL 293

Query: 388 NECRPLSVSMGNAIRFLKNLIAKLPLNLPESEVKATLCSDIDRFINEKIIIADEVIVRHA 447
            +CRPLS SM NAI+ L   I  +  +  E E K+ L + IDR+I EKI++A + I R A
Sbjct: 294 TQCRPLSASMYNAIKLLNKEITGVSGSKREEEAKSELRAAIDRYIKEKIVLAAQAISRFA 353

Query: 448 ATKIRDGDVLLTYGSSCAVEMLLLYAHELGKEFRVVVVDSRPKLEGQALLRRLIGRGLSC 507
             KI +GDV+L YG S  V  +L  A   G++FRVVVVDSRP+LE +  LR L+  G+  
Sbjct: 354 YKKISNGDVILVYGCSSLVSHILQEAWAKGRQFRVVVVDSRPRLEAKHTLRSLVRAGVPA 413

Query: 508 TYTHINAISYMMHKVTRVFLGAAAVLSNGTVYSRVGTAAVAMVANAFHVPVLICCEAYKF 567
           +Y  I A SY++ +V++V LGA A+L+NG+V SRVGTA +A+VA A +VPVL+CCE YKF
Sbjct: 414 SYLLIPAASYVLPEVSKVLLGAHALLANGSVMSRVGTAQLALVARAHNVPVLVCCETYKF 473

Query: 568 HERVQLDSICFNELGDPDAISKVPGRKDINHLENLAAKEQLQLLNLMYDATPSDYISMIV 627
            ERVQ D+   NEL DPD +  +  R +   L N      L+LLNL+YD TP + + +++
Sbjct: 474 CERVQTDAFVSNELDDPDDL--LCERGEHVALANWQNHSSLRLLNLVYDVTPPELVDLVI 508

BLAST of Cucsa.198800 vs. TrEMBL
Match: A0A0A0LSL4_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_1G015840 PE=3 SV=1)

HSP 1 Score: 1244.6 bits (3219), Expect = 0.0e+00
Identity = 625/626 (99.84%), Postives = 626/626 (100.00%), Query Frame = 1

Query: 1   MDARRGSRVVNPKIRQVGFFTLDPPNPPGRSLSGPADCTPSQQSLSPPSNSLSPVMIPPP 60
           MDARRGSRVVNPKIRQVGFFTLDPPNPPGRSLSGPADCTPSQQSLSPPSNSLSPVMIPPP
Sbjct: 1   MDARRGSRVVNPKIRQVGFFTLDPPNPPGRSLSGPADCTPSQQSLSPPSNSLSPVMIPPP 60

Query: 61  RHLSDNLASRSAVFPLPPPANWRDSADHIHVGSYNPSEMLLATSPSGRIGDEELDMSEDS 120
           RHLSDNLASRSAVFPLPPPANWRDSADHIHVGSYNPSEMLLATSPSGRIGDEELDMSEDS
Sbjct: 61  RHLSDNLASRSAVFPLPPPANWRDSADHIHVGSYNPSEMLLATSPSGRIGDEELDMSEDS 120

Query: 121 FTSVPYRRSGSGKIASSFPGVGIYMAAKASETSQAMVPNTVEANKSVGVAEKNGGASTEL 180
           FTSVPYRRSGSGKIASSFPGVGIYMAAKASETSQAMVPNTVEANKSVGVAEKNGGASTEL
Sbjct: 121 FTSVPYRRSGSGKIASSFPGVGIYMAAKASETSQAMVPNTVEANKSVGVAEKNGGASTEL 180

Query: 181 QNRQPPSNSKPQKAKTTKAERRALQEAQRAAKGEAKKSSNAPGKAVKQSSTMRDSTAAPS 240
           QNRQPPSNSKPQKAKTTKAERRALQEAQRAAKGEAKKSSNAPGKAVKQSSTMRDSTAAPS
Sbjct: 181 QNRQPPSNSKPQKAKTTKAERRALQEAQRAAKGEAKKSSNAPGKAVKQSSTMRDSTAAPS 240

Query: 241 VASTDKRGGDRTHDKERKKDAPPPRMQFDDKSRVEKAKKRAVFNQTEARNRVELFRHLPQ 300
           VASTDKRGGDRTHDKERKKDAPPPRMQFDDKSRVEKAKKRAVFNQTEARNRVELFRHLPQ
Sbjct: 241 VASTDKRGGDRTHDKERKKDAPPPRMQFDDKSRVEKAKKRAVFNQTEARNRVELFRHLPQ 300

Query: 301 YEQGTQLPDLVSKFFQLDPIHPAIYKVGLQYMAGDICGGNARCIAMLHAFQQAIKDYSTP 360
           YEQGTQLPDLVSKFFQLDPIHPAIYKVGLQYMAGDICGGNARCIAMLHAFQQAIKDYSTP
Sbjct: 301 YEQGTQLPDLVSKFFQLDPIHPAIYKVGLQYMAGDICGGNARCIAMLHAFQQAIKDYSTP 360

Query: 361 PQKSLGRDLTTKIGGYVSFFNECRPLSVSMGNAIRFLKNLIAKLPLNLPESEVKATLCSD 420
           PQKSLGRDLTTKIGGYVSFFNECRPLSVSMGNAIRFLKNLIAKLPLNLPESEVKATLCSD
Sbjct: 361 PQKSLGRDLTTKIGGYVSFFNECRPLSVSMGNAIRFLKNLIAKLPLNLPESEVKATLCSD 420

Query: 421 IDRFINEKIIIADEVIVRHAATKIRDGDVLLTYGSSCAVEMLLLYAHELGKEFRVVVVDS 480
           IDRFINEKIIIADEVIVRHAATKIRDGDVLLTYGSSCAVEMLLLYAHELGKEFRVVVVDS
Sbjct: 421 IDRFINEKIIIADEVIVRHAATKIRDGDVLLTYGSSCAVEMLLLYAHELGKEFRVVVVDS 480

Query: 481 RPKLEGQALLRRLIGRGLSCTYTHINAISYMMHKVTRVFLGAAAVLSNGTVYSRVGTAAV 540
           RPKLEGQALLRRLIGRGLSCTYTHINAISYMMHKVTRVFLGAAAVLSNGTVYSRVGTAAV
Sbjct: 481 RPKLEGQALLRRLIGRGLSCTYTHINAISYMMHKVTRVFLGAAAVLSNGTVYSRVGTAAV 540

Query: 541 AMVANAFHVPVLICCEAYKFHERVQLDSICFNELGDPDAISKVPGRKDINHLENLAAKEQ 600
           AMVANAFHVPVLICCEAYKFHERVQLDSICFNELGDPDAISKVPGRKDINHLENLAAKEQ
Sbjct: 541 AMVANAFHVPVLICCEAYKFHERVQLDSICFNELGDPDAISKVPGRKDINHLENLAAKEQ 600

Query: 601 LQLLNLMYDATPSDYISMIVTDYGMV 627
           LQLLNLMYDATPSDYISMIVTDYGM+
Sbjct: 601 LQLLNLMYDATPSDYISMIVTDYGMI 626

BLAST of Cucsa.198800 vs. TrEMBL
Match: A0A0A0LPQ4_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_1G015850 PE=3 SV=1)

HSP 1 Score: 1070.5 bits (2767), Expect = 8.4e-310
Identity = 552/635 (86.93%), Postives = 576/635 (90.71%), Query Frame = 1

Query: 1   MDARRGSRVVNPKIRQVGFFTLDPPNPPGRSLSGPADCTPSQQSLSPPSNSLSPVMIPPP 60
           MDARRGSR+VNPK+RQVGFFTLD PN P RSLSGP +C PSQQSLSPP+NSLSPVMIPPP
Sbjct: 1   MDARRGSRIVNPKVRQVGFFTLDAPNSPDRSLSGPPNCLPSQQSLSPPTNSLSPVMIPPP 60

Query: 61  RHLSDNLASRSAVFPLPPPANWRDSADHIHVGSYNPSEMLLATSP----SGRIGDEELDM 120
           RHLSDNLASRSAV P+P PANWR+S D I VGSYNPSE LLATSP    S R GDEELDM
Sbjct: 61  RHLSDNLASRSAVLPVPAPANWRNSTDQIPVGSYNPSETLLATSPMASPSSRTGDEELDM 120

Query: 121 SEDSFTSVPYRRSGSGKIASSFPGVGIYMAAKASETSQAMVPNTVEANKSVGVAEKNGGA 180
           SEDS  SV +RRSGSGKIASSFPGVGI++ AK+ E SQA+   T+E NK  GV EK GG 
Sbjct: 121 SEDSSNSVWHRRSGSGKIASSFPGVGIHVGAKSLENSQAVPLQTLETNKPAGVGEKTGGP 180

Query: 181 STELQNRQPPSNSKPQKAKTTKAERRALQEAQRAAK----GEAKKSSNAPGKAVKQSSTM 240
           STE+QNRQPP NSKP KAKTTKAERRALQEAQRAAK    GEA KS  APGKAVKQ + M
Sbjct: 181 STEVQNRQPPPNSKPLKAKTTKAERRALQEAQRAAKVSAKGEANKSGAAPGKAVKQPAAM 240

Query: 241 RDSTAAPSVASTDKRGG-DRTHDKERKKDAPPPRMQFDDKSRVEKAKKRAVFNQTEARNR 300
           +DSTAAPSVA TDKRGG DRT DKERKKDAPPPRMQFDDKSRVEKAKKRAVFNQTEARNR
Sbjct: 241 KDSTAAPSVAPTDKRGGGDRTLDKERKKDAPPPRMQFDDKSRVEKAKKRAVFNQTEARNR 300

Query: 301 VELFRHLPQYEQGTQLPDLVSKFFQLDPIHPAIYKVGLQYMAGDICGGNARCIAMLHAFQ 360
           VELFRHLPQYEQGTQLPDLVSKFFQLD IHPAIYKVGLQYMA DICGGNARCIAMLHAFQ
Sbjct: 301 VELFRHLPQYEQGTQLPDLVSKFFQLDSIHPAIYKVGLQYMARDICGGNARCIAMLHAFQ 360

Query: 361 QAIKDYSTPPQKSLGRDLTTKIGGYVSFFNECRPLSVSMGNAIRFLKNLIAKLPLNLPES 420
           Q+IKDYSTPPQKSLGRDLT KIG YVSFFNECRPLSVSMGNAIRFLKN IAKLPLNLPES
Sbjct: 361 QSIKDYSTPPQKSLGRDLTAKIGSYVSFFNECRPLSVSMGNAIRFLKNRIAKLPLNLPES 420

Query: 421 EVKATLCSDIDRFINEKIIIADEVIVRHAATKIRDGDVLLTYGSSCAVEMLLLYAHELGK 480
           EVKATLCSDI+RFINEKIIIADEVIVRHAATKIRDGDVLLTYGSS AVEML+LYAHELGK
Sbjct: 421 EVKATLCSDINRFINEKIIIADEVIVRHAATKIRDGDVLLTYGSSSAVEMLILYAHELGK 480

Query: 481 EFRVVVVDSRPKLEGQALLRRLIGRGLSCTYTHINAISYMMHKVTRVFLGAAAVLSNGTV 540
           EFRVVVVDSRPK EG+ALLRRLI RGL+CTYTHINAISY+MH+VTRVFLGAAAVLSNGTV
Sbjct: 481 EFRVVVVDSRPKQEGKALLRRLIARGLNCTYTHINAISYVMHEVTRVFLGAAAVLSNGTV 540

Query: 541 YSRVGTAAVAMVANAFHVPVLICCEAYKFHERVQLDSICFNELGDPDAISKVPGRKDINH 600
           YSRVGTAAVAMVANAFHVPVLICCEAYKFHERVQLDSICFNELGDPD ISKVP RKDIN 
Sbjct: 541 YSRVGTAAVAMVANAFHVPVLICCEAYKFHERVQLDSICFNELGDPDTISKVPERKDIN- 600

Query: 601 LENLAAKEQLQLLNLMYDATPSDYISMIVTDYGMV 627
             +LAAKEQLQLLNLMYDATPSDYISMIVTDYGM+
Sbjct: 601 --DLAAKEQLQLLNLMYDATPSDYISMIVTDYGMI 632

BLAST of Cucsa.198800 vs. TrEMBL
Match: M5WXG3_PRUPE (Uncharacterized protein OS=Prunus persica GN=PRUPE_ppa002668mg PE=3 SV=1)

HSP 1 Score: 842.0 bits (2174), Expect = 4.7e-241
Identity = 451/644 (70.03%), Postives = 514/644 (79.81%), Query Frame = 1

Query: 1   MDARRGSR-VVNPKIRQVGFFTLDPPNPPGRSLSGPADCTPSQQSLSPPSNSLSPVMIPP 60
           MDARR SR V++PK+RQVGFFT +    P R+LSGP   +    S SP SNSL PVMIPP
Sbjct: 1   MDARRASRTVIDPKVRQVGFFTPNAETAPSRTLSGPPIASSPPLSSSPASNSLYPVMIPP 60

Query: 61  PRHLSDNLASRSAVFPLPPPANWRDSA-DHIHVGSYNPSEMLLATSP-----SGRIGDEE 120
            RHLSD +A R+A  P+P  A+ R  A + + VGSYNPSE LL TSP     S R+GD  
Sbjct: 61  SRHLSDTIAIRTAALPVPESASRRKEAGERVPVGSYNPSESLLGTSPVTASPSSRVGDAT 120

Query: 121 LDMSEDSFTSVPYRRSGSGKIASSFPGVGIYMAAKASETSQAMVPNTVEANKSVGVAEKN 180
           L+ S++S     YRRS SGK+ASSFPG G   A             TV+A   + V EK+
Sbjct: 121 LEFSDESSVRW-YRRSDSGKLASSFPGGGFDSA-------------TVKA--PLEVPEKS 180

Query: 181 GGASTELQNRQPPSNSKPQKAKTT-KAERRALQEAQRAAKGEAKKSSN---------APG 240
           G   ++ Q ++ PSNS+P K+KTT KAERRALQEAQRAAK  AK             APG
Sbjct: 181 GEEVSKEQ-KEVPSNSEPLKSKTTTKAERRALQEAQRAAKAAAKAEGKKPAAVTRGGAPG 240

Query: 241 KAVKQSSTMRDST-AAPSVASTDKRGGDRTHDKERKKDAPPPRMQFDDKSRVEKAKKRAV 300
           K  KQS   RD    A SVA++DK+GGDR  +KERKKD PPPRMQFDDK+RV+KAK+RAV
Sbjct: 241 KPAKQSLQKRDPPPVASSVAASDKKGGDRPPEKERKKDVPPPRMQFDDKNRVDKAKRRAV 300

Query: 301 FNQTEARNRVELFRHLPQYEQGTQLPDLVSKFFQLDPIHPAIYKVGLQYMAGDICGGNAR 360
            NQTEA+NRVELFRHLPQYE GTQLPDL SKFFQLDP+HPAIYKVGLQY+AG+I GGNAR
Sbjct: 301 VNQTEAKNRVELFRHLPQYEHGTQLPDLESKFFQLDPMHPAIYKVGLQYLAGEISGGNAR 360

Query: 361 CIAMLHAFQQAIKDYSTPPQKSLGRDLTTKIGGYVSFFNECRPLSVSMGNAIRFLKNLIA 420
           CIAML AFQ+AI+DY+TPP+K+L RDLT KI  YVSFF ECRPLS+SMGNAIRFLK+ IA
Sbjct: 361 CIAMLQAFQEAIRDYTTPPEKTLVRDLTAKISSYVSFFIECRPLSISMGNAIRFLKSRIA 420

Query: 421 KLPLNLPESEVKATLCSDIDRFINEKIIIADEVIVRHAATKIRDGDVLLTYGSSCAVEML 480
           KLPL L ESE K+TLCS+IDRFINEKII+AD+VIVRHAATK+RDGDVLLTYGSSC VEM+
Sbjct: 421 KLPLTLSESEAKSTLCSEIDRFINEKIILADKVIVRHAATKVRDGDVLLTYGSSCVVEMI 480

Query: 481 LLYAHELGKEFRVVVVDSRPKLEGQALLRRLIGRGLSCTYTHINAISYMMHKVTRVFLGA 540
           LLYAHELGK+FRVVVVDSRPKLEGQALLRRL+ +GLSCTYTHINA+SY+MH+VTRVFLGA
Sbjct: 481 LLYAHELGKQFRVVVVDSRPKLEGQALLRRLVAKGLSCTYTHINAVSYIMHEVTRVFLGA 540

Query: 541 AAVLSNGTVYSRVGTAAVAMVANAFHVPVLICCEAYKFHERVQLDSICFNELGDPDAISK 600
           A+VLSNGTVYSRVGTA VAMVA+AF VPVLICCEAYKFHERVQLDSIC NELGDPDAI+K
Sbjct: 541 ASVLSNGTVYSRVGTACVAMVAHAFCVPVLICCEAYKFHERVQLDSICSNELGDPDAIAK 600

Query: 601 VPGRKDINHLENLAAKEQLQLLNLMYDATPSDYISMIVTDYGMV 627
           V GR D+N+L+N A KE LQLLNLMYDATPSDYISMIVTDYGM+
Sbjct: 601 VSGRTDVNYLDNWANKENLQLLNLMYDATPSDYISMIVTDYGMI 627

BLAST of Cucsa.198800 vs. TrEMBL
Match: W9S6A9_9ROSA (Translation initiation factor eIF-2B subunit delta OS=Morus notabilis GN=L484_022286 PE=3 SV=1)

HSP 1 Score: 840.1 bits (2169), Expect = 1.8e-240
Identity = 443/645 (68.68%), Postives = 513/645 (79.53%), Query Frame = 1

Query: 1   MDARRGSRVVN-PKIRQVGFFTLDPPNPPGRSLSGPADCTPSQQSLSPPSNSLSPVMIPP 60
           MDARR SR V+ PK+R VGFFT + P  P R+ SGP        S SP SNS SPVMIPP
Sbjct: 1   MDARRSSRAVSDPKVRHVGFFTPNAPPLPSRTQSGPPAAGSPPLSESPASNSFSPVMIPP 60

Query: 61  PRHLSDNLASRSAVFPLPPPANWRDSA-DHIHVGSYNPSEMLLATSP----SGRIGDEEL 120
           PRHLSD +A R+A  P+P  A  R +A DH+ VGSYNPSE LL TSP    S   GD EL
Sbjct: 61  PRHLSD-VAERTAAVPVPDSAYRRTAAGDHVPVGSYNPSESLLGTSPVASPSSVAGDGEL 120

Query: 121 DMSEDSFTSVPYRRSGSGKIASSFPGVGIYMA-AKASETSQAMVPNTVEAN---KSVGVA 180
           + S +S  S  +R   SGK ASSFP  G  +  AK SE S     N   A+   K + V 
Sbjct: 121 EFSGESSASW-HRWRDSGKFASSFPSGGFELTMAKGSENSATKGKNLAAASNAKKPLDVP 180

Query: 181 EKNGGASTELQNRQPPSNSKPQKAKTTKAERRALQEAQRAAKGEAKKSSN--------AP 240
           EK+GG  TE+QN   P+ SKP KAK+TKAERRALQEAQRAAK   K+ S         AP
Sbjct: 181 EKSGGVVTEVQNELQPT-SKPLKAKSTKAERRALQEAQRAAKAATKEGSKSATVSRGVAP 240

Query: 241 GKAVKQSSTMRD-STAAPSVASTDKRGGDRTHDKERKKDAPPPRMQFDDKSRVEKAKKRA 300
            K VKQ S  +D S+ APSVA+++K+GGDR  +KERKKD PPPRMQFDDK+RVEKAKKR+
Sbjct: 241 SKPVKQPSQRKDASSVAPSVAASEKKGGDRAPEKERKKDVPPPRMQFDDKNRVEKAKKRS 300

Query: 301 VFNQTEARNRVELFRHLPQYEQGTQLPDLVSKFFQLDPIHPAIYKVGLQYMAGDICGGNA 360
           VF+QTEARNRVELFRHLPQYE GTQLPDL SKFFQL+P+HPAIYKVGLQY+AGDICGGN 
Sbjct: 301 VFHQTEARNRVELFRHLPQYEHGTQLPDLESKFFQLEPMHPAIYKVGLQYLAGDICGGNG 360

Query: 361 RCIAMLHAFQQAIKDYSTPPQKSLGRDLTTKIGGYVSFFNECRPLSVSMGNAIRFLKNLI 420
           RC+AML AF++ I+DYSTPP+K+L RDLT KI  YVSFF ECRP+S+SMGNAIRF+K+ I
Sbjct: 361 RCVAMLKAFEEVIRDYSTPPEKALSRDLTAKISSYVSFFTECRPISISMGNAIRFIKSRI 420

Query: 421 AKLPLNLPESEVKATLCSDIDRFINEKIIIADEVIVRHAATKIRDGDVLLTYGSSCAVEM 480
           AKLPL L ESE KA LC+DI+ FINEKI++AD VIV+HAA+K++DGDVLLTYGSSC VEM
Sbjct: 421 AKLPLFLSESEAKAALCADINSFINEKILLADRVIVKHAASKVKDGDVLLTYGSSCVVEM 480

Query: 481 LLLYAHELGKEFRVVVVDSRPKLEGQALLRRLIGRGLSCTYTHINAISYMMHKVTRVFLG 540
           +LL+AHELGK+FRVV+VDSRPK EGQALL RL+ +GLSCTYTHINA+SY+MH+VT+V LG
Sbjct: 481 ILLHAHELGKQFRVVIVDSRPKFEGQALLCRLVAKGLSCTYTHINAVSYVMHEVTKVLLG 540

Query: 541 AAAVLSNGTVYSRVGTAAVAMVANAFHVPVLICCEAYKFHERVQLDSICFNELGDPDAIS 600
           AA+VLSNGTVYSRVGTA+VAMVA+AF VPVLICCEAYKFHERVQLDSIC NELGD DAI+
Sbjct: 541 AASVLSNGTVYSRVGTASVAMVAHAFCVPVLICCEAYKFHERVQLDSICSNELGDTDAIA 600

Query: 601 KVPGRKDINHLENLAAKEQLQLLNLMYDATPSDYISMIVTDYGMV 627
           KVPGR D+N+LENLA KE LQLLNLMYDATPSDY+SMI+TDYGM+
Sbjct: 601 KVPGRMDVNYLENLAKKENLQLLNLMYDATPSDYVSMIITDYGMI 642

BLAST of Cucsa.198800 vs. TrEMBL
Match: A0A067JJB6_JATCU (Uncharacterized protein OS=Jatropha curcas GN=JCGZ_05844 PE=3 SV=1)

HSP 1 Score: 813.5 bits (2100), Expect = 1.8e-232
Identity = 442/648 (68.21%), Postives = 509/648 (78.55%), Query Frame = 1

Query: 1   MDARRGSRVVNPKIRQVGFFTLDPPNPPGRSLSGPADCTPSQQSLSPPSNSLSPVMIPPP 60
           M+ARRG R V PK+RQVGFFT + P PP RS SGP D    Q + SP SNSLSPVMIPPP
Sbjct: 1   MEARRGWRAV-PKVRQVGFFTPNEPLPPTRSQSGPPDSNSPQLANSPASNSLSPVMIPPP 60

Query: 61  RHLSDNLASRS-AVFPLPPPANWRDS---ADHIH-VGSYNPSEMLLATSP-SGRIGDEEL 120
           RH+SDNLA R+ +  P+P    +R S    DH+  VGSYNP+E LL  SP S RI D E 
Sbjct: 61  RHMSDNLAHRATSPLPVPEAPAFRRSITGGDHVTLVGSYNPTESLLGQSPPSARIVDGEF 120

Query: 121 DMSEDSFTSVPYRRSGSGKIASSFPGVGIYMAA-KASETSQAMVPNTVEANKSVGVAEKN 180
             SE+S      RRS S K ASSFP  G  + + K+SE  +      V+  K VG  EK 
Sbjct: 121 --SEESSPGW-LRRSNSAKFASSFPSGGFDLTSLKSSENLE------VQEKKPVGGPEKG 180

Query: 181 GGASTELQNRQPPSNSKPQKAKTTKAERRALQEAQRAAKGEAKKSSNAPGK--------- 240
           GG   ELQN   PS SKP KAKTT+AERRALQE+QRAAK  AK +  A GK         
Sbjct: 181 GGV-VELQNELQPS-SKPLKAKTTRAERRALQESQRAAKAAAKATGEAVGKPAILSEGAA 240

Query: 241 ----AVKQSSTMRDST--AAPSVASTDKRGGDRTHDKERKKDAPPPRMQFDDKSRVEKAK 300
               A +Q S  +D T  AA S+A++D++G DR  +KER+++ P PRMQFDD++RVEKAK
Sbjct: 241 ASKPAKQQPSQKKDVTPPAASSIAASDRKGVDRPPEKERRREVPAPRMQFDDRTRVEKAK 300

Query: 301 KRAVFNQTEARNRVELFRHLPQYEQGTQLPDLVSKFFQLDPIHPAIYKVGLQYMAGDICG 360
           KRAV NQTEARNRVELFRHLPQYE GTQLPDL SK FQLDP+HPAIYK GL+Y+AGDI G
Sbjct: 301 KRAVVNQTEARNRVELFRHLPQYEHGTQLPDLESKIFQLDPMHPAIYKAGLKYLAGDIIG 360

Query: 361 GNARCIAMLHAFQQAIKDYSTPPQKSLGRDLTTKIGGYVSFFNECRPLSVSMGNAIRFLK 420
           GNARCIAML AFQ+ IKDYSTPP+KSL RDLT K+G Y+SF  ECRPLS+SMGNAIRFLK
Sbjct: 361 GNARCIAMLQAFQEVIKDYSTPPEKSLTRDLTAKLGSYISFLIECRPLSMSMGNAIRFLK 420

Query: 421 NLIAKLPLNLPESEVKATLCSDIDRFINEKIIIADEVIVRHAATKIRDGDVLLTYGSSCA 480
           + IAKLPL   ESE KA+L SDIDRFINEKI++AD+VIVRHAA+KIRDGDVLLTYGSSC 
Sbjct: 421 SRIAKLPLTFSESEAKASLRSDIDRFINEKIVLADKVIVRHAASKIRDGDVLLTYGSSCV 480

Query: 481 VEMLLLYAHELGKEFRVVVVDSRPKLEGQALLRRLIGRGLSCTYTHINAISYMMHKVTRV 540
           VEM+LLYAHELGK+FRVV+VDSRPKLEG+ALLR L+ +GL+CTYTHINA+SY+MH+VTRV
Sbjct: 481 VEMILLYAHELGKQFRVVIVDSRPKLEGRALLRTLVAKGLNCTYTHINAVSYIMHEVTRV 540

Query: 541 FLGAAAVLSNGTVYSRVGTAAVAMVANAFHVPVLICCEAYKFHERVQLDSICFNELGDPD 600
           FLGA++VLSNGTVYSRVGTA VAMVA+AFHVPVLICCEAYKFHERVQLDSIC NELGDP+
Sbjct: 541 FLGASSVLSNGTVYSRVGTACVAMVAHAFHVPVLICCEAYKFHERVQLDSICSNELGDPN 600

Query: 601 AISKVPGRKDINHLENLAAKEQLQLLNLMYDATPSDYISMIVTDYGMV 627
           AI+KV GR D+N+L+ LA KE LQLLNLMYDATPS+YISMIVTDYGM+
Sbjct: 601 AIAKVSGRTDVNYLDTLANKENLQLLNLMYDATPSEYISMIVTDYGMI 636

BLAST of Cucsa.198800 vs. TAIR10
Match: AT5G38640.1 (AT5G38640.1 NagB/RpiA/CoA transferase-like superfamily protein)

HSP 1 Score: 702.6 bits (1812), Expect = 2.3e-202
Identity = 390/652 (59.82%), Postives = 465/652 (71.32%), Query Frame = 1

Query: 1   MDARRGSR-VVNPKIRQVGFFTLDPPNPPGRSLS----GPADCTPSQQSLSPPSNSLSPV 60
           M++RR  R VV+PK+RQVGFFT   P+   +            + S  ++SP  NSLSPV
Sbjct: 1   MESRRNPRAVVDPKVRQVGFFTSSQPDSSTQQQQPDPIAATSASSSSSTISPSGNSLSPV 60

Query: 61  MIPPPRHLSDNLASRSAVFPLPPPANWRDSADHIHVGSYNPSEMLLATSPSGRIGDEELD 120
           MIPPPR       + S  FPL            + V     S    A SPS        D
Sbjct: 61  MIPPPR------LNSSDTFPL-----------RVAVAGAAAS----AVSPSSFSYSSRRD 120

Query: 121 MSEDSFTSVPYRRS------GSGKIASSFPGVGI--YMAAKASETSQAMVPNTVEANKSV 180
             + S T+ P R S       S  +ASS PG+G+    AAK+S    + +          
Sbjct: 121 FPDGSTTASPGRHSRGSFVKSSVPVASSLPGIGMDSMAAAKSSSVPASGLTTVSVVTMPP 180

Query: 181 GVAEKNGGASTELQNRQPPSNSKPQKAKTTKAERRALQEAQRAAKGEAK---------KS 240
           G++EK  G   E+Q+ Q     KP K KTTKAERRA+QEAQRAAK  AK          S
Sbjct: 181 GLSEKASGM--EVQSDQ----KKPLKEKTTKAERRAIQEAQRAAKAAAKADGGKVAPVSS 240

Query: 241 SNAPGKAVKQS----STMRDSTAAPSVASTDKRGGDRTHDKERKKDAPPPRMQFDDKSRV 300
           ++    +VK +    +T + +  A +  + +K+GG    +K+RKKDAP PRMQ+DD+SRV
Sbjct: 241 ASVASTSVKAAKPAKATSQKNDVAVATGAAEKKGGPS--EKDRKKDAPHPRMQYDDESRV 300

Query: 301 EKAKKRAVFNQTEARNRVELFRHLPQYEQGTQLPDLVSKFFQLDPIHPAIYKVGLQYMAG 360
            KAK+RAV  QTEA+NRVELFRHLPQYE GTQLPDL +KFFQLDP+HPA+YKVGLQY++G
Sbjct: 301 VKAKRRAVVKQTEAKNRVELFRHLPQYEHGTQLPDLETKFFQLDPMHPAVYKVGLQYLSG 360

Query: 361 DICGGNARCIAMLHAFQQAIKDYSTPPQKSLGRDLTTKIGGYVSFFNECRPLSVSMGNAI 420
           DI GGNARCIAML AFQ+ +KDYSTPP+KSL RD+T KI  YVSF  ECRPLS+SMGNAI
Sbjct: 361 DISGGNARCIAMLQAFQEVVKDYSTPPEKSLNRDMTAKISSYVSFLIECRPLSISMGNAI 420

Query: 421 RFLKNLIAKLPLNLPESEVKATLCSDIDRFINEKIIIADEVIVRHAATKIRDGDVLLTYG 480
           RF+KN IAKLP+ L ESE KATL SDI+RFINEKII+AD VIV+HA TKIRDGDVLLTYG
Sbjct: 421 RFVKNRIAKLPITLSESEAKATLQSDIERFINEKIIVADTVIVKHAVTKIRDGDVLLTYG 480

Query: 481 SSCAVEMLLLYAHELGKEFRVVVVDSRPKLEGQALLRRLIGRGLSCTYTHINAISYMMHK 540
           S  AVEM++L+AHEL K+FRV+VVDSRPKLEGQ LLRRLI RG++CTYTHINAISY+MH+
Sbjct: 481 SPTAVEMVILHAHELRKKFRVLVVDSRPKLEGQLLLRRLIKRGINCTYTHINAISYIMHE 540

Query: 541 VTRVFLGAAAVLSNGTVYSRVGTAAVAMVANAFHVPVLICCEAYKFHERVQLDSICFNEL 600
           VT+VFLGA++VLSNGTVYSRVGTA VAMVANAF VPVL+CCEAYKFHERVQLDSIC NEL
Sbjct: 541 VTKVFLGASSVLSNGTVYSRVGTACVAMVANAFRVPVLVCCEAYKFHERVQLDSICSNEL 600

Query: 601 GDPDAISKVPGRKDINHLENLAAKEQLQLLNLMYDATPSDYISMIVTDYGMV 627
           GDP+AIS+V GR+DIN+L+ L     LQ LNLMYDATPSDYISMI+TDYGMV
Sbjct: 601 GDPNAISEVHGREDINYLDGLTNNANLQFLNLMYDATPSDYISMIITDYGMV 623

BLAST of Cucsa.198800 vs. TAIR10
Match: AT1G48970.1 (AT1G48970.1 NagB/RpiA/CoA transferase-like superfamily protein)

HSP 1 Score: 684.1 bits (1764), Expect = 8.3e-197
Identity = 372/656 (56.71%), Postives = 460/656 (70.12%), Query Frame = 1

Query: 1   MDARRGSRVVNPKIRQVGFFTLDPPNP------PGRSLSGPADCTPSQQSLS--PPSNSL 60
           MD RRG+  V PK+R+VGFFT   P P      P RS SGP D T S   LS  P  N +
Sbjct: 1   MDTRRGALAV-PKVRRVGFFTSIEPPPESSLQRPNRSQSGPVDATTSSSPLSDSPSGNLI 60

Query: 61  SPVMIPPPRHLSDNLASR-----SAVFPLPPPANWR----DSADHIHVGSYNPSEMLLAT 120
           SPV+IPP RH SDNL SR     +A  P+P PA +R         +HVGSYNP + LL T
Sbjct: 61  SPVLIPPSRHHSDNLTSRVAAAAAAPVPVPGPAAFRRYLTPERTLLHVGSYNPPDSLLGT 120

Query: 121 S-PSGRIGDEELDMSEDSFTSVPYRRSGSGKIASSFP--GVGIYMAAKASETSQAMVPNT 180
           S PS   G     +SEDS +   ++RS S K+++S P  G  + +A +A + S+  +  T
Sbjct: 121 SSPSSNGG-----LSEDSASLFGFQRSDSTKLSASLPNGGFDLTLAVRAPQESETKIATT 180

Query: 181 VEANKSVGVAEKNGGASTELQNRQPPSNSKPQKAKTTKAERRALQEAQRAAKGEAKKSSN 240
             +     +AE +G + +     Q     K  K KTTKAERRA+QEAQRAAK  AK   +
Sbjct: 181 SASQGKKKIAEVSGKSESATTKPQKEKEPKALKDKTTKAERRAIQEAQRAAKAAAKGEGS 240

Query: 241 A---------PGKAVKQSSTMRDSTAAPSVASTDKRGGDRTHDKERKKDAPPPRMQFDDK 300
                     PGKA  +    ++    P  +S  ++      +KE++ D P  +MQ+DDK
Sbjct: 241 RRADESGRANPGKAAIKPQPKKERL--PVTSSVSEKTAVAV-EKEKRMDVPQTQMQYDDK 300

Query: 301 SRVEKAKKRAVFNQTEARNRVELFRHLPQYEQGTQLPDLVSKFFQLDPIHPAIYKVGLQY 360
           SRV+KAK+RAV  QTE++N+VELF HLPQYE+  QLP+L S FF LD IH A+YKVGLQ+
Sbjct: 301 SRVDKAKRRAVVEQTESKNKVELFLHLPQYERSNQLPNLSSNFFTLDSIHHAVYKVGLQH 360

Query: 361 MAGDICGGNARCIAMLHAFQQAIKDYSTPPQKSLGRDLTTKIGGYVSFFNECRPLSVSMG 420
           +AGDI G NARCIAML AFQ+AI+DYSTPP K L  DLT KI GYVSF  ECRPLS+SMG
Sbjct: 361 LAGDISGDNARCIAMLQAFQEAIEDYSTPPMKDLTMDLTAKINGYVSFLIECRPLSMSMG 420

Query: 421 NAIRFLKNLIAKLPLNLPESEVKATLCSDIDRFINEKIIIADEVIVRHAATKIRDGDVLL 480
           NAIRFLKN I KLP+NL ESE K++LCSDI RFI+EK IIAD+ IV+HA TKIRDG+VLL
Sbjct: 421 NAIRFLKNQIRKLPVNLSESEAKSSLCSDIGRFIDEKTIIADKAIVQHAVTKIRDGEVLL 480

Query: 481 TYGSSCAVEMLLLYAHELGKEFRVVVVDSRPKLEGQALLRRLIGRGLSCTYTHINAISYM 540
           TYG SC VEM+LLYAHE+GK+FRVV+VDSRP LEGQ LLRRL+ RGL CTYTHINAISY+
Sbjct: 481 TYGFSCVVEMILLYAHEIGKKFRVVIVDSRPNLEGQKLLRRLVTRGLDCTYTHINAISYI 540

Query: 541 MHKVTRVFLGAAAVLSNGTVYSRVGTAAVAMVANAFHVPVLICCEAYKFHERVQLDSICF 600
           M + TRVFLGA+++ SNGT+Y+RVGT+ +AMVANAF VPV++CCEAYKFHERV LDSIC 
Sbjct: 541 MREATRVFLGASSIYSNGTLYARVGTSCIAMVANAFSVPVIVCCEAYKFHERVLLDSICS 600

Query: 601 NELGDPDAISKVPG-RKDINHLENLAAKEQLQLLNLMYDATPSDYISMIVTDYGMV 627
           NELGDPDA++ +P  R +  H + +   + LQ LNLMYD+TPS+YISMIV+DYGM+
Sbjct: 601 NELGDPDAVANIPSFRTNAKHSKTMDNNKNLQFLNLMYDSTPSEYISMIVSDYGMI 647

BLAST of Cucsa.198800 vs. TAIR10
Match: AT2G44070.1 (AT2G44070.1 NagB/RpiA/CoA transferase-like superfamily protein)

HSP 1 Score: 466.8 bits (1200), Expect = 2.1e-131
Identity = 230/308 (74.68%), Postives = 267/308 (86.69%), Query Frame = 1

Query: 320 IHPAIYKVGLQYMAGDICGGNARCIAMLHAFQQAIKDYSTPPQKSLGRDLTTKIGGYVSF 379
           +H A+YKVGL Y++ DI GGNARCIAML AFQ+ +KDYSTPP+ +L RD+T +I  YVSF
Sbjct: 1   MHSAVYKVGLHYLSWDISGGNARCIAMLQAFQEVVKDYSTPPENTLNRDMTAQISSYVSF 60

Query: 380 FNECRPLSVSMGNAIRFLKNLIAKLPLNLPESEVKATLCSDIDRFINEKIIIADEVIVRH 439
             ECRPLS+SMGNAIRF+KN IAKLP+ L ESE KA+L SDI+RFINEKII AD VIV H
Sbjct: 61  LIECRPLSISMGNAIRFVKNRIAKLPITLSESEAKASLHSDIERFINEKIIGADTVIVEH 120

Query: 440 AATKIRDGDVLLTYGSSCAVEMLLLYAHELGKEFRVVVVDSRPKLEGQALLRRLIGRGLS 499
           A TKIRDGDVLLTYGSS  +EM+L++AHELGK+FRV VVDSRPKL+G+ LLRRLI RG++
Sbjct: 121 AVTKIRDGDVLLTYGSSTVIEMILVHAHELGKKFRVFVVDSRPKLQGKLLLRRLIKRGIN 180

Query: 500 CTYTHINAISYMMHKVTRVFLGAAAVLSNGTVYSRVGTAAVAMVANAFHVPVLICCEAYK 559
           CTYTHI AISY+MH+VT+VFLGA++V SNGTVYSRVGTA VAMVANAF VPVL+CCEAYK
Sbjct: 181 CTYTHITAISYIMHQVTKVFLGASSVFSNGTVYSRVGTACVAMVANAFRVPVLVCCEAYK 240

Query: 560 FHERVQLD-SICFNELGDPDAISKVPGRKDINHLENLAAKEQLQLLNLMYDATPSDYISM 619
           FHE+VQLD SIC NELGDP+AISKV GR+DIN+L  L    +L+ LNLMYDATPSDYISM
Sbjct: 241 FHEKVQLDYSICSNELGDPNAISKVHGREDINYLNGLTNNAKLKFLNLMYDATPSDYISM 300

Query: 620 IVTDYGMV 627
           I+TDYGMV
Sbjct: 301 IITDYGMV 308

BLAST of Cucsa.198800 vs. TAIR10
Match: AT3G07300.3 (AT3G07300.3 NagB/RpiA/CoA transferase-like superfamily protein)

HSP 1 Score: 90.9 bits (224), Expect = 3.1e-18
Identity = 64/214 (29.91%), Postives = 101/214 (47.20%), Query Frame = 1

Query: 412 EVKATLCSDIDRFINEKIIIADEVIVRHAATKIRDGDVLLTYGSSCAVEMLLLYAHELGK 471
           ++K  +   +++ I E I    E I   A   I   +V+LT GSS  V   L  A E  +
Sbjct: 177 KLKHDVIEGVNQLIQE-IAGCHEQIAEQAIEHIHQNEVILTLGSSRTVLEFLCAAKEKKR 236

Query: 472 EFRVVVVDSRPKLEGQALLRRLIGRGLSCTYTHINAISYMMHKVTRVFLGAAAVLSNGTV 531
            FRV V +  P+ +G  L + L+ RGL  T    +A+  M+ +V  V +GA AV++NG V
Sbjct: 237 SFRVFVAEGAPRYQGHLLAKELVARGLQTTVITDSAVFAMISRVNMVIIGAHAVMANGGV 296

Query: 532 YSRVGTAAVAMVANAFHVPVLICCEAYKFHERVQLD-SICFNELGDPDAISKVPGRKDIN 591
              VG    A+ A    VP ++   ++K       +  +  NEL  P  +       D  
Sbjct: 297 IGPVGVNMAALAAQKHAVPFVVLAGSHKLCPLYPHNPEVLLNELRSPSELLDFGEFSDC- 356

Query: 592 HLENLAAKEQLQLLNLMYDATPSDYISMIVTDYG 625
            L+  A    LQ++N  +D  P + +S+ +TD G
Sbjct: 357 -LDFGAGSGSLQVVNPTFDYVPPNLVSLFITDTG 387

BLAST of Cucsa.198800 vs. TAIR10
Match: AT1G72340.1 (AT1G72340.1 NagB/RpiA/CoA transferase-like superfamily protein)

HSP 1 Score: 73.6 bits (179), Expect = 5.2e-13
Identity = 53/196 (27.04%), Postives = 91/196 (46.43%), Query Frame = 1

Query: 432 ADEVIVRHAATKIRDGDVLLTYGSSCAVEMLLLYAHELGKEFRVVVVDSRPKLEGQALLR 491
           A  +I   +   I DG  +L +G S  V  +L  A +  K FRV+  + RP   G  L  
Sbjct: 187 ARRIIAMLSQDFIFDGCTILVHGLSRVVLEILKTAAQNNKLFRVLCTEGRPDGTGVLLSS 246

Query: 492 RLIGRGLSCTYTHINAISYMMHKVTRVFLGAAAVLSNGTVYSRVGTAAVAMVANAFHVPV 551
            L    +       +A++Y M +V  VF+GA  V+ +G + + +GT  +A+VA++ + PV
Sbjct: 247 ELSKLDIPVKLLLDSAVAYSMDEVDMVFVGADGVVESGGIINMMGTYQIALVAHSMNKPV 306

Query: 552 LICCEAYKFHERVQLDSICFNELGDPDAISKVPGRKDINHLENLAAKEQLQLLNLMYDAT 611
            +  E+YKF     LD                P  + I     +  K +++      D T
Sbjct: 307 YVAAESYKFARLYPLDQ-----------KDMAPALRPIEFGVKIPTKVEVE--RSARDYT 366

Query: 612 PSDYISMIVTDYGMVN 628
           P  Y++++ TD G+++
Sbjct: 367 PPQYLTLLFTDLGVLS 369

BLAST of Cucsa.198800 vs. NCBI nr
Match: gi|778656268|ref|XP_011648725.1| (PREDICTED: translation initiation factor eIF-2B subunit delta-like [Cucumis sativus])

HSP 1 Score: 1244.6 bits (3219), Expect = 0.0e+00
Identity = 625/626 (99.84%), Postives = 626/626 (100.00%), Query Frame = 1

Query: 1   MDARRGSRVVNPKIRQVGFFTLDPPNPPGRSLSGPADCTPSQQSLSPPSNSLSPVMIPPP 60
           MDARRGSRVVNPKIRQVGFFTLDPPNPPGRSLSGPADCTPSQQSLSPPSNSLSPVMIPPP
Sbjct: 1   MDARRGSRVVNPKIRQVGFFTLDPPNPPGRSLSGPADCTPSQQSLSPPSNSLSPVMIPPP 60

Query: 61  RHLSDNLASRSAVFPLPPPANWRDSADHIHVGSYNPSEMLLATSPSGRIGDEELDMSEDS 120
           RHLSDNLASRSAVFPLPPPANWRDSADHIHVGSYNPSEMLLATSPSGRIGDEELDMSEDS
Sbjct: 61  RHLSDNLASRSAVFPLPPPANWRDSADHIHVGSYNPSEMLLATSPSGRIGDEELDMSEDS 120

Query: 121 FTSVPYRRSGSGKIASSFPGVGIYMAAKASETSQAMVPNTVEANKSVGVAEKNGGASTEL 180
           FTSVPYRRSGSGKIASSFPGVGIYMAAKASETSQAMVPNTVEANKSVGVAEKNGGASTEL
Sbjct: 121 FTSVPYRRSGSGKIASSFPGVGIYMAAKASETSQAMVPNTVEANKSVGVAEKNGGASTEL 180

Query: 181 QNRQPPSNSKPQKAKTTKAERRALQEAQRAAKGEAKKSSNAPGKAVKQSSTMRDSTAAPS 240
           QNRQPPSNSKPQKAKTTKAERRALQEAQRAAKGEAKKSSNAPGKAVKQSSTMRDSTAAPS
Sbjct: 181 QNRQPPSNSKPQKAKTTKAERRALQEAQRAAKGEAKKSSNAPGKAVKQSSTMRDSTAAPS 240

Query: 241 VASTDKRGGDRTHDKERKKDAPPPRMQFDDKSRVEKAKKRAVFNQTEARNRVELFRHLPQ 300
           VASTDKRGGDRTHDKERKKDAPPPRMQFDDKSRVEKAKKRAVFNQTEARNRVELFRHLPQ
Sbjct: 241 VASTDKRGGDRTHDKERKKDAPPPRMQFDDKSRVEKAKKRAVFNQTEARNRVELFRHLPQ 300

Query: 301 YEQGTQLPDLVSKFFQLDPIHPAIYKVGLQYMAGDICGGNARCIAMLHAFQQAIKDYSTP 360
           YEQGTQLPDLVSKFFQLDPIHPAIYKVGLQYMAGDICGGNARCIAMLHAFQQAIKDYSTP
Sbjct: 301 YEQGTQLPDLVSKFFQLDPIHPAIYKVGLQYMAGDICGGNARCIAMLHAFQQAIKDYSTP 360

Query: 361 PQKSLGRDLTTKIGGYVSFFNECRPLSVSMGNAIRFLKNLIAKLPLNLPESEVKATLCSD 420
           PQKSLGRDLTTKIGGYVSFFNECRPLSVSMGNAIRFLKNLIAKLPLNLPESEVKATLCSD
Sbjct: 361 PQKSLGRDLTTKIGGYVSFFNECRPLSVSMGNAIRFLKNLIAKLPLNLPESEVKATLCSD 420

Query: 421 IDRFINEKIIIADEVIVRHAATKIRDGDVLLTYGSSCAVEMLLLYAHELGKEFRVVVVDS 480
           IDRFINEKIIIADEVIVRHAATKIRDGDVLLTYGSSCAVEMLLLYAHELGKEFRVVVVDS
Sbjct: 421 IDRFINEKIIIADEVIVRHAATKIRDGDVLLTYGSSCAVEMLLLYAHELGKEFRVVVVDS 480

Query: 481 RPKLEGQALLRRLIGRGLSCTYTHINAISYMMHKVTRVFLGAAAVLSNGTVYSRVGTAAV 540
           RPKLEGQALLRRLIGRGLSCTYTHINAISYMMHKVTRVFLGAAAVLSNGTVYSRVGTAAV
Sbjct: 481 RPKLEGQALLRRLIGRGLSCTYTHINAISYMMHKVTRVFLGAAAVLSNGTVYSRVGTAAV 540

Query: 541 AMVANAFHVPVLICCEAYKFHERVQLDSICFNELGDPDAISKVPGRKDINHLENLAAKEQ 600
           AMVANAFHVPVLICCEAYKFHERVQLDSICFNELGDPDAISKVPGRKDINHLENLAAKEQ
Sbjct: 541 AMVANAFHVPVLICCEAYKFHERVQLDSICFNELGDPDAISKVPGRKDINHLENLAAKEQ 600

Query: 601 LQLLNLMYDATPSDYISMIVTDYGMV 627
           LQLLNLMYDATPSDYISMIVTDYGM+
Sbjct: 601 LQLLNLMYDATPSDYISMIVTDYGMI 626

BLAST of Cucsa.198800 vs. NCBI nr
Match: gi|659106505|ref|XP_008453358.1| (PREDICTED: translation initiation factor eIF-2B subunit delta-like isoform X2 [Cucumis melo])

HSP 1 Score: 1218.8 bits (3152), Expect = 0.0e+00
Identity = 612/626 (97.76%), Postives = 617/626 (98.56%), Query Frame = 1

Query: 1   MDARRGSRVVNPKIRQVGFFTLDPPNPPGRSLSGPADCTPSQQSLSPPSNSLSPVMIPPP 60
           MDARRGSRVVNPK+RQVGFFTLD PNPPGRSLSGPADCTPSQQSLSPPSNSLSPVMIPPP
Sbjct: 1   MDARRGSRVVNPKVRQVGFFTLDAPNPPGRSLSGPADCTPSQQSLSPPSNSLSPVMIPPP 60

Query: 61  RHLSDNLASRSAVFPLPPPANWRDSADHIHVGSYNPSEMLLATSPSGRIGDEELDMSEDS 120
           RHLSDNLASRSAVFPLPPPANWRDSADHIHVGSYNPSEMLLATSPSGRIGDEELDMSEDS
Sbjct: 61  RHLSDNLASRSAVFPLPPPANWRDSADHIHVGSYNPSEMLLATSPSGRIGDEELDMSEDS 120

Query: 121 FTSVPYRRSGSGKIASSFPGVGIYMAAKASETSQAMVPNTVEANKSVGVAEKNGGASTEL 180
            TSVPYRRSGSGKIASSFPGVGIYMAAKASETSQAMV NTVEANKSVGVAEKNGGASTE+
Sbjct: 121 STSVPYRRSGSGKIASSFPGVGIYMAAKASETSQAMVVNTVEANKSVGVAEKNGGASTEV 180

Query: 181 QNRQPPSNSKPQKAKTTKAERRALQEAQRAAKGEAKKSSNAPGKAVKQSSTMRDSTAAPS 240
           QNRQPPSNSKP KAKTTKAERRALQEAQRAAKGE KKS N PGKAVKQSSTMRDSTAAPS
Sbjct: 181 QNRQPPSNSKPLKAKTTKAERRALQEAQRAAKGEVKKSGNTPGKAVKQSSTMRDSTAAPS 240

Query: 241 VASTDKRGGDRTHDKERKKDAPPPRMQFDDKSRVEKAKKRAVFNQTEARNRVELFRHLPQ 300
           VASTDKRGGDRTHDKERKKDAPPPRMQFDDKSRVEKAKKRAVFNQTEARNRVELFRHLPQ
Sbjct: 241 VASTDKRGGDRTHDKERKKDAPPPRMQFDDKSRVEKAKKRAVFNQTEARNRVELFRHLPQ 300

Query: 301 YEQGTQLPDLVSKFFQLDPIHPAIYKVGLQYMAGDICGGNARCIAMLHAFQQAIKDYSTP 360
           YEQGTQLPDLVSKFFQLDPIHPAIYKVGLQYMAGDICGGNARCIAMLHAFQQAIKDYSTP
Sbjct: 301 YEQGTQLPDLVSKFFQLDPIHPAIYKVGLQYMAGDICGGNARCIAMLHAFQQAIKDYSTP 360

Query: 361 PQKSLGRDLTTKIGGYVSFFNECRPLSVSMGNAIRFLKNLIAKLPLNLPESEVKATLCSD 420
           PQKSLGRDLTTKIGGYVSFFNECRPLSVSMGNAIRFLKNLIAKLPLNLPESEVKATLCSD
Sbjct: 361 PQKSLGRDLTTKIGGYVSFFNECRPLSVSMGNAIRFLKNLIAKLPLNLPESEVKATLCSD 420

Query: 421 IDRFINEKIIIADEVIVRHAATKIRDGDVLLTYGSSCAVEMLLLYAHELGKEFRVVVVDS 480
           IDRFINEKIIIADEVIVRHAATKIRDGDVLLTYGSSC+VEMLLLYAHELGKEFRVVVVDS
Sbjct: 421 IDRFINEKIIIADEVIVRHAATKIRDGDVLLTYGSSCSVEMLLLYAHELGKEFRVVVVDS 480

Query: 481 RPKLEGQALLRRLIGRGLSCTYTHINAISYMMHKVTRVFLGAAAVLSNGTVYSRVGTAAV 540
           RPKLEGQALLRRLIGRGLSCTYTHINAISY+MHKVTRVFLGAAAVLSNGTVYSRVGTAAV
Sbjct: 481 RPKLEGQALLRRLIGRGLSCTYTHINAISYVMHKVTRVFLGAAAVLSNGTVYSRVGTAAV 540

Query: 541 AMVANAFHVPVLICCEAYKFHERVQLDSICFNELGDPDAISKVPGRKDINHLENLAAKEQ 600
           AMVANAFHVPVLICCEAYKFHERVQLDSICFNELGDPDAISKV GRKDINHLENLAAKEQ
Sbjct: 541 AMVANAFHVPVLICCEAYKFHERVQLDSICFNELGDPDAISKVQGRKDINHLENLAAKEQ 600

Query: 601 LQLLNLMYDATPSDYISMIVTDYGMV 627
           LQ LNLMYDATPSDYISMIVTDYGM+
Sbjct: 601 LQFLNLMYDATPSDYISMIVTDYGMI 626

BLAST of Cucsa.198800 vs. NCBI nr
Match: gi|659106503|ref|XP_008453356.1| (PREDICTED: translation initiation factor eIF-2B subunit delta-like isoform X1 [Cucumis melo])

HSP 1 Score: 1213.0 bits (3137), Expect = 0.0e+00
Identity = 612/630 (97.14%), Postives = 617/630 (97.94%), Query Frame = 1

Query: 1   MDARRGSRVVNPKIRQVGFFTLDPPNPPGRSLSGPADCTPSQQSLSPPSNSLSPVMIPPP 60
           MDARRGSRVVNPK+RQVGFFTLD PNPPGRSLSGPADCTPSQQSLSPPSNSLSPVMIPPP
Sbjct: 1   MDARRGSRVVNPKVRQVGFFTLDAPNPPGRSLSGPADCTPSQQSLSPPSNSLSPVMIPPP 60

Query: 61  RHLSDNLASRSAVFPLPPPANWRDSADHIHVGSYNPSEMLLATSPSGRIGDEELDMSEDS 120
           RHLSDNLASRSAVFPLPPPANWRDSADHIHVGSYNPSEMLLATSPSGRIGDEELDMSEDS
Sbjct: 61  RHLSDNLASRSAVFPLPPPANWRDSADHIHVGSYNPSEMLLATSPSGRIGDEELDMSEDS 120

Query: 121 FTSVPYRRSGSGKIASSFPGVGIYMAAKASETSQAMVPNTVEANKSVGVAEKNGGASTEL 180
            TSVPYRRSGSGKIASSFPGVGIYMAAKASETSQAMV NTVEANKSVGVAEKNGGASTE+
Sbjct: 121 STSVPYRRSGSGKIASSFPGVGIYMAAKASETSQAMVVNTVEANKSVGVAEKNGGASTEV 180

Query: 181 QNRQPPSNSKPQKAKTTKAERRALQEAQRAAKG----EAKKSSNAPGKAVKQSSTMRDST 240
           QNRQPPSNSKP KAKTTKAERRALQEAQRAAKG    E KKS N PGKAVKQSSTMRDST
Sbjct: 181 QNRQPPSNSKPLKAKTTKAERRALQEAQRAAKGSANGEVKKSGNTPGKAVKQSSTMRDST 240

Query: 241 AAPSVASTDKRGGDRTHDKERKKDAPPPRMQFDDKSRVEKAKKRAVFNQTEARNRVELFR 300
           AAPSVASTDKRGGDRTHDKERKKDAPPPRMQFDDKSRVEKAKKRAVFNQTEARNRVELFR
Sbjct: 241 AAPSVASTDKRGGDRTHDKERKKDAPPPRMQFDDKSRVEKAKKRAVFNQTEARNRVELFR 300

Query: 301 HLPQYEQGTQLPDLVSKFFQLDPIHPAIYKVGLQYMAGDICGGNARCIAMLHAFQQAIKD 360
           HLPQYEQGTQLPDLVSKFFQLDPIHPAIYKVGLQYMAGDICGGNARCIAMLHAFQQAIKD
Sbjct: 301 HLPQYEQGTQLPDLVSKFFQLDPIHPAIYKVGLQYMAGDICGGNARCIAMLHAFQQAIKD 360

Query: 361 YSTPPQKSLGRDLTTKIGGYVSFFNECRPLSVSMGNAIRFLKNLIAKLPLNLPESEVKAT 420
           YSTPPQKSLGRDLTTKIGGYVSFFNECRPLSVSMGNAIRFLKNLIAKLPLNLPESEVKAT
Sbjct: 361 YSTPPQKSLGRDLTTKIGGYVSFFNECRPLSVSMGNAIRFLKNLIAKLPLNLPESEVKAT 420

Query: 421 LCSDIDRFINEKIIIADEVIVRHAATKIRDGDVLLTYGSSCAVEMLLLYAHELGKEFRVV 480
           LCSDIDRFINEKIIIADEVIVRHAATKIRDGDVLLTYGSSC+VEMLLLYAHELGKEFRVV
Sbjct: 421 LCSDIDRFINEKIIIADEVIVRHAATKIRDGDVLLTYGSSCSVEMLLLYAHELGKEFRVV 480

Query: 481 VVDSRPKLEGQALLRRLIGRGLSCTYTHINAISYMMHKVTRVFLGAAAVLSNGTVYSRVG 540
           VVDSRPKLEGQALLRRLIGRGLSCTYTHINAISY+MHKVTRVFLGAAAVLSNGTVYSRVG
Sbjct: 481 VVDSRPKLEGQALLRRLIGRGLSCTYTHINAISYVMHKVTRVFLGAAAVLSNGTVYSRVG 540

Query: 541 TAAVAMVANAFHVPVLICCEAYKFHERVQLDSICFNELGDPDAISKVPGRKDINHLENLA 600
           TAAVAMVANAFHVPVLICCEAYKFHERVQLDSICFNELGDPDAISKV GRKDINHLENLA
Sbjct: 541 TAAVAMVANAFHVPVLICCEAYKFHERVQLDSICFNELGDPDAISKVQGRKDINHLENLA 600

Query: 601 AKEQLQLLNLMYDATPSDYISMIVTDYGMV 627
           AKEQLQ LNLMYDATPSDYISMIVTDYGM+
Sbjct: 601 AKEQLQFLNLMYDATPSDYISMIVTDYGMI 630

BLAST of Cucsa.198800 vs. NCBI nr
Match: gi|659106513|ref|XP_008453361.1| (PREDICTED: translation initiation factor eIF-2B subunit delta-like [Cucumis melo])

HSP 1 Score: 1084.7 bits (2804), Expect = 0.0e+00
Identity = 555/635 (87.40%), Postives = 580/635 (91.34%), Query Frame = 1

Query: 1   MDARRGSRVVNPKIRQVGFFTLDPPNPPGRSLSGPADCTPSQQSLSPPSNSLSPVMIPPP 60
           MDARRGSR+VNPK+RQVGFFTLD PN P RSLSGP +C PSQQSLSPP+NSLSPVMIPPP
Sbjct: 1   MDARRGSRIVNPKVRQVGFFTLDAPNSPDRSLSGPPNCLPSQQSLSPPTNSLSPVMIPPP 60

Query: 61  RHLSDNLASRSAVFPLPPPANWRDSADHIHVGSYNPSEMLLATSP----SGRIGDEELDM 120
           RHLSDNLASRSAV P+P PANWR+S D I VGSYNPSE LLATSP    S R GDEELDM
Sbjct: 61  RHLSDNLASRSAVLPVPAPANWRNSTDQIPVGSYNPSETLLATSPMASPSSRTGDEELDM 120

Query: 121 SEDSFTSVPYRRSGSGKIASSFPGVGIYMAAKASETSQAMVP-NTVEANKSVGVAEKNGG 180
           SEDS  SV +RRSGSGKIASSFPGVGI+M AK+ E SQA+VP  T+E NK  GV EK GG
Sbjct: 121 SEDSSNSVWHRRSGSGKIASSFPGVGIHMGAKSLENSQAVVPLQTLETNKPAGVGEKTGG 180

Query: 181 ASTELQNRQPPSNSKPQKAKTTKAERRALQEAQRAAK----GEAKKSSNAPGKAVKQSST 240
            STE+QNRQPP NSKP KAKTTKAERRALQEAQRAAK    GEA KS  A GKAVKQ + 
Sbjct: 181 PSTEVQNRQPPPNSKPLKAKTTKAERRALQEAQRAAKVSAKGEANKSGAASGKAVKQPAA 240

Query: 241 MRDSTAAPSVASTDKRGGDRTHDKERKKDAPPPRMQFDDKSRVEKAKKRAVFNQTEARNR 300
           M+DSTAAPSVA TDKRGGDRT DKERKKDAPPPR+QFDDKSRVEKAKKRAVFNQTEARNR
Sbjct: 241 MKDSTAAPSVAPTDKRGGDRTLDKERKKDAPPPRLQFDDKSRVEKAKKRAVFNQTEARNR 300

Query: 301 VELFRHLPQYEQGTQLPDLVSKFFQLDPIHPAIYKVGLQYMAGDICGGNARCIAMLHAFQ 360
           VELFRHLPQYEQGTQLPDLVSKFFQLD IHPA+YKVGLQYMA DICGGNARCIAMLHAFQ
Sbjct: 301 VELFRHLPQYEQGTQLPDLVSKFFQLDSIHPAVYKVGLQYMARDICGGNARCIAMLHAFQ 360

Query: 361 QAIKDYSTPPQKSLGRDLTTKIGGYVSFFNECRPLSVSMGNAIRFLKNLIAKLPLNLPES 420
           QAIKDYSTPPQKSLGRDLT KIG YVSFFNECRPLSVSMGNAIRFLKN IAKLPLNLPES
Sbjct: 361 QAIKDYSTPPQKSLGRDLTAKIGSYVSFFNECRPLSVSMGNAIRFLKNRIAKLPLNLPES 420

Query: 421 EVKATLCSDIDRFINEKIIIADEVIVRHAATKIRDGDVLLTYGSSCAVEMLLLYAHELGK 480
           EVKATLCSDI+RFINEKIIIADEVIVRHAATKIRDGDVLLTYGSS AVEML+LYAHELGK
Sbjct: 421 EVKATLCSDINRFINEKIIIADEVIVRHAATKIRDGDVLLTYGSSSAVEMLILYAHELGK 480

Query: 481 EFRVVVVDSRPKLEGQALLRRLIGRGLSCTYTHINAISYMMHKVTRVFLGAAAVLSNGTV 540
           EFRVVVVDSRPK EG+ALLRRLI RGL+CTYTHINAISYMMH+VTRVFLGAAAVLSNGTV
Sbjct: 481 EFRVVVVDSRPKQEGKALLRRLIARGLNCTYTHINAISYMMHEVTRVFLGAAAVLSNGTV 540

Query: 541 YSRVGTAAVAMVANAFHVPVLICCEAYKFHERVQLDSICFNELGDPDAISKVPGRKDINH 600
           YSRVGTAAVAMVANAFHVPVLICCEAYKFHERVQLDSICFNELGDPD ISKVP RKDIN+
Sbjct: 541 YSRVGTAAVAMVANAFHVPVLICCEAYKFHERVQLDSICFNELGDPDTISKVPERKDINY 600

Query: 601 LENLAAKEQLQLLNLMYDATPSDYISMIVTDYGMV 627
           L++LAAKEQLQLLNLMYDATPSDYISMIVTDYGM+
Sbjct: 601 LDDLAAKEQLQLLNLMYDATPSDYISMIVTDYGMI 635

BLAST of Cucsa.198800 vs. NCBI nr
Match: gi|778656271|ref|XP_004138224.2| (PREDICTED: translation initiation factor eIF-2B subunit delta-like isoform X1 [Cucumis sativus])

HSP 1 Score: 1070.5 bits (2767), Expect = 1.2e-309
Identity = 552/635 (86.93%), Postives = 576/635 (90.71%), Query Frame = 1

Query: 1   MDARRGSRVVNPKIRQVGFFTLDPPNPPGRSLSGPADCTPSQQSLSPPSNSLSPVMIPPP 60
           MDARRGSR+VNPK+RQVGFFTLD PN P RSLSGP +C PSQQSLSPP+NSLSPVMIPPP
Sbjct: 1   MDARRGSRIVNPKVRQVGFFTLDAPNSPDRSLSGPPNCLPSQQSLSPPTNSLSPVMIPPP 60

Query: 61  RHLSDNLASRSAVFPLPPPANWRDSADHIHVGSYNPSEMLLATSP----SGRIGDEELDM 120
           RHLSDNLASRSAV P+P PANWR+S D I VGSYNPSE LLATSP    S R GDEELDM
Sbjct: 61  RHLSDNLASRSAVLPVPAPANWRNSTDQIPVGSYNPSETLLATSPMASPSSRTGDEELDM 120

Query: 121 SEDSFTSVPYRRSGSGKIASSFPGVGIYMAAKASETSQAMVPNTVEANKSVGVAEKNGGA 180
           SEDS  SV +RRSGSGKIASSFPGVGI++ AK+ E SQA+   T+E NK  GV EK GG 
Sbjct: 121 SEDSSNSVWHRRSGSGKIASSFPGVGIHVGAKSLENSQAVPLQTLETNKPAGVGEKTGGP 180

Query: 181 STELQNRQPPSNSKPQKAKTTKAERRALQEAQRAAK----GEAKKSSNAPGKAVKQSSTM 240
           STE+QNRQPP NSKP KAKTTKAERRALQEAQRAAK    GEA KS  APGKAVKQ + M
Sbjct: 181 STEVQNRQPPPNSKPLKAKTTKAERRALQEAQRAAKVSAKGEANKSGAAPGKAVKQPAAM 240

Query: 241 RDSTAAPSVASTDKRGG-DRTHDKERKKDAPPPRMQFDDKSRVEKAKKRAVFNQTEARNR 300
           +DSTAAPSVA TDKRGG DRT DKERKKDAPPPRMQFDDKSRVEKAKKRAVFNQTEARNR
Sbjct: 241 KDSTAAPSVAPTDKRGGGDRTLDKERKKDAPPPRMQFDDKSRVEKAKKRAVFNQTEARNR 300

Query: 301 VELFRHLPQYEQGTQLPDLVSKFFQLDPIHPAIYKVGLQYMAGDICGGNARCIAMLHAFQ 360
           VELFRHLPQYEQGTQLPDLVSKFFQLD IHPAIYKVGLQYMA DICGGNARCIAMLHAFQ
Sbjct: 301 VELFRHLPQYEQGTQLPDLVSKFFQLDSIHPAIYKVGLQYMARDICGGNARCIAMLHAFQ 360

Query: 361 QAIKDYSTPPQKSLGRDLTTKIGGYVSFFNECRPLSVSMGNAIRFLKNLIAKLPLNLPES 420
           Q+IKDYSTPPQKSLGRDLT KIG YVSFFNECRPLSVSMGNAIRFLKN IAKLPLNLPES
Sbjct: 361 QSIKDYSTPPQKSLGRDLTAKIGSYVSFFNECRPLSVSMGNAIRFLKNRIAKLPLNLPES 420

Query: 421 EVKATLCSDIDRFINEKIIIADEVIVRHAATKIRDGDVLLTYGSSCAVEMLLLYAHELGK 480
           EVKATLCSDI+RFINEKIIIADEVIVRHAATKIRDGDVLLTYGSS AVEML+LYAHELGK
Sbjct: 421 EVKATLCSDINRFINEKIIIADEVIVRHAATKIRDGDVLLTYGSSSAVEMLILYAHELGK 480

Query: 481 EFRVVVVDSRPKLEGQALLRRLIGRGLSCTYTHINAISYMMHKVTRVFLGAAAVLSNGTV 540
           EFRVVVVDSRPK EG+ALLRRLI RGL+CTYTHINAISY+MH+VTRVFLGAAAVLSNGTV
Sbjct: 481 EFRVVVVDSRPKQEGKALLRRLIARGLNCTYTHINAISYVMHEVTRVFLGAAAVLSNGTV 540

Query: 541 YSRVGTAAVAMVANAFHVPVLICCEAYKFHERVQLDSICFNELGDPDAISKVPGRKDINH 600
           YSRVGTAAVAMVANAFHVPVLICCEAYKFHERVQLDSICFNELGDPD ISKVP RKDIN 
Sbjct: 541 YSRVGTAAVAMVANAFHVPVLICCEAYKFHERVQLDSICFNELGDPDTISKVPERKDIN- 600

Query: 601 LENLAAKEQLQLLNLMYDATPSDYISMIVTDYGMV 627
             +LAAKEQLQLLNLMYDATPSDYISMIVTDYGM+
Sbjct: 601 --DLAAKEQLQLLNLMYDATPSDYISMIVTDYGMI 632