BLAST of Cucsa.194320 vs. Swiss-Prot
Match:
NCED6_ARATH (9-cis-epoxycarotenoid dioxygenase NCED6, chloroplastic OS=Arabidopsis thaliana GN=NCED6 PE=2 SV=1)
HSP 1 Score: 696.8 bits (1797), Expect = 1.9e-199
Identity = 334/540 (61.85%), Postives = 428/540 (79.26%), Query Frame = 1
Query: 13 TLCRSSSTIETP-PIRKLKRRLNPLQKFAASLLDAVESSLFVDK-----LPKTIDPAVQI 72
TL +S + +P ++ LN LQK AA++LD +ESS+ + LPK DPAVQ+
Sbjct: 42 TLPDLTSPVPSPVKLKPTYPNLNLLQKLAATMLDKIESSIVIPMEQNRPLPKPTDPAVQL 101
Query: 73 SGNFSPVPECSVRHDLEIIGNLPACLRGIYLRNGANPMHAPTGGHHLFDGDGMIHAVTFH 132
SGNF+PV EC V++ LE++G +P+CL+G+Y+RNGANPM P GHHLFDGDGMIHAV+
Sbjct: 102 SGNFAPVNECPVQNGLEVVGQIPSCLKGVYIRNGANPMFPPLAGHHLFDGDGMIHAVSIG 161
Query: 133 SGNKASYSCRFTRTNRLQQEAALGRLVFPKPIGELHGHQGLARLAIFLARAGIGLIDGSK 192
N+ SYSCR+T+TNRL QE ALGR VFPKPIGELHGH GLARLA+F ARAGIGL+DG++
Sbjct: 162 FDNQVSYSCRYTKTNRLVQETALGRSVFPKPIGELHGHSGLARLALFTARAGIGLVDGTR 221
Query: 193 GTGVANAGLVYFNGRLLALSEDDLPYHVQIKDDGDLETIGRFNFNGQINCPMIAHPKVDP 252
G GVANAG+V+FNGRLLA+SEDDLPY V+I GDLETIGRF F+ QI+ +IAHPKVD
Sbjct: 222 GMGVANAGVVFFNGRLLAMSEDDLPYQVKIDGQGDLETIGRFGFDDQIDSSVIAHPKVDA 281
Query: 253 ISGDLHGLSYNMIKKPYLKYLRFDRFGKKSRDVDITLREPTMIHDFAITENHVVIPDHQV 312
+GDLH LSYN++KKP+L+YL+F+ GKK+RDV+ITL EPTMIHDFAITEN VVIPD Q+
Sbjct: 282 TTGDLHTLSYNVLKKPHLRYLKFNTCGKKTRDVEITLPEPTMIHDFAITENFVVIPDQQM 341
Query: 313 VFKLLEMVRGGSPVVFDPKKTSRFGILPKSGVDEKGIVWIEVPNCFCFHLWNAWEETGGN 372
VFKL EM+RGGSPV++ +K +RFG+L K + I W++VP+CFCFHLWNAWEE
Sbjct: 342 VFKLSEMIRGGSPVIYVKEKMARFGVLSKQDLTGSDINWVDVPDCFCFHLWNAWEERTEE 401
Query: 373 DEKSIVVVGSCMNPPDSIFNDRDQPLRIELTEIRMDVKSRKVTRRVFGSGMNLEAGQMNR 432
+ IVV+GSCM+PPD+IF++ +P R+EL+EIR+++++++ R+V +G+NLEAG +NR
Sbjct: 402 GDPVIVVIGSCMSPPDTIFSESGEPTRVELSEIRLNMRTKESNRKVIVTGVNLEAGHINR 461
Query: 433 GLVGRKTRFVYMAIADPWPKCSGIAKVDLETGKVKKFLYGEGRYGGEPFYVPENGNWGGD 492
VGRK++FVY+AIADPWPKCSGIAKVD++ G V +F YG R+GGEP +VPE G+
Sbjct: 462 SYVGRKSQFVYIAIADPWPKCSGIAKVDIQNGTVSEFNYGPSRFGGEPCFVPE-----GE 521
Query: 493 NEKEDGGYIVGFVRDEKRERSEVVVVKAAEMEEVAAVRLPVRVPYGFHGTFVSEEELNGQ 547
E ED GY++GFVRDE+++ SE VVV A +M++VAAVRLP RVPYGFHGTFVSE +L Q
Sbjct: 522 GE-EDKGYVMGFVRDEEKDESEFVVVDATDMKQVAAVRLPERVPYGFHGTFVSENQLKEQ 575
BLAST of Cucsa.194320 vs. Swiss-Prot
Match:
NCED9_ARATH (9-cis-epoxycarotenoid dioxygenase NCED9, chloroplastic OS=Arabidopsis thaliana GN=NCED9 PE=2 SV=1)
HSP 1 Score: 647.1 bits (1668), Expect = 1.7e-184
Identity = 320/553 (57.87%), Postives = 408/553 (73.78%), Query Frame = 1
Query: 4 SLQSP---PPPLTLCRSSSTIETPPIRKLKRRLNPLQKFAASLLDAVESSLFVDKL---- 63
SLQSP PP + T P +LNP+Q+ AA +LDAVE+++ +
Sbjct: 113 SLQSPTFKPPSWKKLCNDVTNLIPKTTNQNPKLNPVQRTAAMVLDAVENAMISHERRRHP 172
Query: 64 -PKTIDPAVQISGNFSPVPECSVRHDLEIIGNLPACLRGIYLRNGANPMHAPTGGHHLFD 123
PKT DPAVQI+GNF PVPE V H+L + G +P C++G+Y+RNGANP+H P GHHLFD
Sbjct: 173 HPKTADPAVQIAGNFFPVPEKPVVHNLPVTGTVPECIQGVYVRNGANPLHKPVSGHHLFD 232
Query: 124 GDGMIHAVTFHSGNKASYSCRFTRTNRLQQEAALGRLVFPKPIGELHGHQGLARLAIFLA 183
GDGM+HAV F +G+ SY+CRFT TNRL QE GR VFPK IGELHGH G+A+L +F
Sbjct: 233 GDGMVHAVRFDNGS-VSYACRFTETNRLVQERECGRPVFPKAIGELHGHLGIAKLMLFNT 292
Query: 184 RAGIGLIDGSKGTGVANAGLVYFNGRLLALSEDDLPYHVQIKDDGDLETIGRFNFNGQIN 243
R GL+D + G GVANAGLVYFNG LLA+SEDDLPYHV++ GDLET GR++F+GQ+
Sbjct: 293 RGLFGLVDPTGGLGVANAGLVYFNGHLLAMSEDDLPYHVKVTQTGDLETSGRYDFDGQLK 352
Query: 244 CPMIAHPKVDPISGDLHGLSYNMIKKPYLKYLRFDRFGKKSRDVDITLREPTMIHDFAIT 303
MIAHPK+DP + +L LSY+++ KPYLKY RF G+KS DV+I L +PTMIHDFAIT
Sbjct: 353 STMIAHPKIDPETRELFALSYDVVSKPYLKYFRFTSDGEKSPDVEIPLDQPTMIHDFAIT 412
Query: 304 ENHVVIPDHQVVFKLLEMVRGGSPVVFDPKKTSRFGILPKSGVDEKGIVWIEVPNCFCFH 363
EN VVIPD QVVF+L EM+RGGSPVV+D KK SRFGIL K+ D I WIEVP+CFCFH
Sbjct: 413 ENFVVIPDQQVVFRLPEMIRGGSPVVYDEKKKSRFGILNKNAKDASSIQWIEVPDCFCFH 472
Query: 364 LWNAWEETGGNDEKSIVVVGSCMNPPDSIFNDRDQPLRIELTEIRMDVKSRKVTRR-VFG 423
LWN+WEE + +VV+GSCM PPDSIFN+ D+ L+ L+EIR+++K+ + TRR V
Sbjct: 473 LWNSWEEP---ETDEVVVIGSCMTPPDSIFNEHDETLQSVLSEIRLNLKTGESTRRPVIS 532
Query: 424 SGMNLEAGQMNRGLVGRKTRFVYMAIADPWPKCSGIAKVDLETGKVKKFLYGEGRYGGEP 483
+NLEAG +NR L+GRKTR+ Y+A+ +PWPK SG AKVDL TG+++K++YGEG+YGGEP
Sbjct: 533 EQVNLEAGMVNRNLLGRKTRYAYLALTEPWPKVSGFAKVDLSTGEIRKYIYGEGKYGGEP 592
Query: 484 FYVPENGNWGGDNEKEDGGYIVGFVRDEKRERSEVVVVKAAEMEEVAAVRLPVRVPYGFH 543
++P GD E EDGGYI+ FV DE++ +SE+ ++ A M+ A V LP RVPYGFH
Sbjct: 593 LFLP-----SGDGE-EDGGYIMVFVHDEEKVKSELQLINAVNMKLEATVTLPSRVPYGFH 652
Query: 544 GTFVSEEELNGQA 548
GTF+S+E+L+ QA
Sbjct: 653 GTFISKEDLSKQA 655
BLAST of Cucsa.194320 vs. Swiss-Prot
Match:
NCED1_MAIZE (9-cis-epoxycarotenoid dioxygenase 1, chloroplastic OS=Zea mays GN=VP14 PE=1 SV=2)
HSP 1 Score: 639.8 bits (1649), Expect = 2.7e-182
Identity = 310/527 (58.82%), Postives = 389/527 (73.81%), Query Frame = 1
Query: 30 KRRLNPLQKFAASLLDAVESSLFVDKL------PKTIDPAVQISGNFSPVPECSVRHDLE 89
K++LN Q+ AA+ LDA E + L P T DPAVQI+GNF+PV E H+L
Sbjct: 81 KKQLNLFQRAAAAALDAFEEGFVANVLERPHGLPSTADPAVQIAGNFAPVGERPPVHELP 140
Query: 90 IIGNLPACLRGIYLRNGANPMHAPTGGHHLFDGDGMIHAVTFHSGNKASYSCRFTRTNRL 149
+ G +P + G+Y RNGANP P GHHLFDGDGM+HA+ +G SY+CRFT T RL
Sbjct: 141 VSGRIPPFIDGVYARNGANPCFDPVAGHHLFDGDGMVHALRIRNGAAESYACRFTETARL 200
Query: 150 QQEAALGRLVFPKPIGELHGHQGLARLAIFLARAGIGLIDGSKGTGVANAGLVYFNGRLL 209
+QE A+GR VFPK IGELHGH G+ARLA+F ARA GL+D S GTGVANAGLVYFNGRLL
Sbjct: 201 RQERAIGRPVFPKAIGELHGHSGIARLALFYARAACGLVDPSAGTGVANAGLVYFNGRLL 260
Query: 210 ALSEDDLPYHVQIKDDGDLETIGRFNFNGQINCPMIAHPKVDPISGDLHGLSYNMIKKPY 269
A+SEDDLPYHV++ DDGDLET+GR++F+GQ+ C MIAHPK+DP +G+LH LSY++IK+PY
Sbjct: 261 AMSEDDLPYHVRVADDGDLETVGRYDFDGQLGCAMIAHPKLDPATGELHALSYDVIKRPY 320
Query: 270 LKYLRFDRFGKKSRDVDITLREPTMIHDFAITENHVVIPDHQVVFKLLEMVRGGSPVVFD 329
LKY F G KS DV+I L +PTMIHDFAITEN VV+PDHQVVFKL EM+RGGSPVV D
Sbjct: 321 LKYFYFRPDGTKSDDVEIPLEQPTMIHDFAITENLVVVPDHQVVFKLQEMLRGGSPVVLD 380
Query: 330 PKKTSRFGILPKSGVDEKGIVWIEVPNCFCFHLWNAWEETGGNDEKSIVVVGSCMNPPDS 389
+KTSRFG+LPK D + W++VP+CFCFHLWNAWE+ + +VV+GSCM P DS
Sbjct: 381 KEKTSRFGVLPKHAADASEMAWVDVPDCFCFHLWNAWEDEATGE---VVVIGSCMTPADS 440
Query: 390 IFNDRDQPLRIELTEIRMDVKSRKVTRRVF---GSGMNLEAGQMNRGLVGRKTRFVYMAI 449
IFN+ D+ L LTEIR+D ++ + TRR +NLE G +NR L+GR+TR+ Y+A+
Sbjct: 441 IFNESDERLESVLTEIRLDARTGRSTRRAVLPPSQQVNLEVGMVNRNLLGRETRYAYLAV 500
Query: 450 ADPWPKCSGIAKVDLETGKVKKFLYGEGRYGGEPFYVPENGNWGGDNEKEDGGYIVGFVR 509
A+PWPK SG AKVDL TG++ KF YGEGR+GGEP +VP + ED GY++ FV
Sbjct: 501 AEPWPKVSGFAKVDLSTGELTKFEYGEGRFGGEPCFVPMDPA-AAHPRGEDDGYVLTFVH 560
Query: 510 DEKRERSEVVVVKAAEMEEVAAVRLPVRVPYGFHGTFVSEEELNGQA 548
DE+ SE++VV AA+M A V+LP RVP+GFHGTF++ +EL QA
Sbjct: 561 DERAGTSELLVVNAADMRLEATVQLPSRVPFGFHGTFITGQELEAQA 603
BLAST of Cucsa.194320 vs. Swiss-Prot
Match:
NCED3_ARATH (9-cis-epoxycarotenoid dioxygenase NCED3, chloroplastic OS=Arabidopsis thaliana GN=NCED3 PE=2 SV=1)
HSP 1 Score: 634.8 bits (1636), Expect = 8.8e-181
Identity = 316/555 (56.94%), Postives = 403/555 (72.61%), Query Frame = 1
Query: 4 SLQSPPP---PLTLCRSSSTIETPPIRKLK-RRLNPLQKFAASLLDAVESSLF----VDK 63
+L +PP P S + + P ++ +++N Q+ AA+ LDA E L +
Sbjct: 53 ALHTPPALHFPKQSSNSPAIVVKPKAKESNTKQMNLFQRAAAAALDAAEGFLVSHEKLHP 112
Query: 64 LPKTIDPAVQISGNFSPVPECSVRHDLEIIGNLPACLRGIYLRNGANPMHAPTGGHHLFD 123
LPKT DP+VQI+GNF+PV E VR +L ++G LP ++G+Y+RNGANP+H P GHH FD
Sbjct: 113 LPKTADPSVQIAGNFAPVNEQPVRRNLPVVGKLPDSIKGVYVRNGANPLHEPVTGHHFFD 172
Query: 124 GDGMIHAVTFHSGNKASYSCRFTRTNRLQQEAALGRLVFPKPIGELHGHQGLARLAIFLA 183
GDGM+HAV F G+ ASY+CRFT+TNR QE LGR VFPK IGELHGH G+ARL +F A
Sbjct: 173 GDGMVHAVKFEHGS-ASYACRFTQTNRFVQERQLGRPVFPKAIGELHGHTGIARLMLFYA 232
Query: 184 RAGIGLIDGSKGTGVANAGLVYFNGRLLALSEDDLPYHVQIKDDGDLETIGRFNFNGQIN 243
RA G++D + GTGVANAGLVYFNGRLLA+SEDDLPY VQI +GDL+T+GRF+F+GQ+
Sbjct: 233 RAAAGIVDPAHGTGVANAGLVYFNGRLLAMSEDDLPYQVQITPNGDLKTVGRFDFDGQLE 292
Query: 244 CPMIAHPKVDPISGDLHGLSYNMIKKPYLKYLRFDRFGKKSRDVDITLREPTMIHDFAIT 303
MIAHPKVDP SG+L LSY+++ KPYLKY RF G KS DV+I L +PTM+HDFAIT
Sbjct: 293 STMIAHPKVDPESGELFALSYDVVSKPYLKYFRFSPDGTKSPDVEIQLDQPTMMHDFAIT 352
Query: 304 ENHVVIPDHQVVFKLLEMVRGGSPVVFDPKKTSRFGILPKSGVDEKGIVWIEVPNCFCFH 363
EN VV+PD QVVFKL EM+RGGSPVV+D K +RFGIL K D I WI+ P+CFCFH
Sbjct: 353 ENFVVVPDQQVVFKLPEMIRGGSPVVYDKNKVARFGILDKYAEDSSNIKWIDAPDCFCFH 412
Query: 364 LWNAWEETGGNDEKSIVVVGSCMNPPDSIFNDRDQPLRIELTEIRMDVKSRKVTRRVFGS 423
LWNAWEE + +VV+GSCM PPDSIFN+ D+ L+ L+EIR+++K+ + TRR S
Sbjct: 413 LWNAWEEP---ETDEVVVIGSCMTPPDSIFNESDENLKSVLSEIRLNLKTGESTRRPIIS 472
Query: 424 G----MNLEAGQMNRGLVGRKTRFVYMAIADPWPKCSGIAKVDLETGKVKKFLYGEGRYG 483
+NLEAG +NR ++GRKT+F Y+A+A+PWPK SG AKVDL TG+VKK LYG+ RYG
Sbjct: 473 NEDQQVNLEAGMVNRNMLGRKTKFAYLALAEPWPKVSGFAKVDLTTGEVKKHLYGDNRYG 532
Query: 484 GEPFYVPENGNWGGDNEKEDGGYIVGFVRDEKRERSEVVVVKAAEMEEVAAVRLPVRVPY 543
GEP ++P G+ +ED GYI+ FV DEK +SE+ +V A +E A V+LP RVPY
Sbjct: 533 GEPLFLP------GEGGEEDEGYILCFVHDEKTWKSELQIVNAVSLEVEATVKLPSRVPY 592
Query: 544 GFHGTFVSEEELNGQ 547
GFHGTF+ ++L Q
Sbjct: 593 GFHGTFIGADDLAKQ 597
BLAST of Cucsa.194320 vs. Swiss-Prot
Match:
NCED_ONCHC (9-cis-epoxycarotenoid dioxygenase, chloroplastic OS=Oncidium hybrid cultivar GN=NCED PE=2 SV=1)
HSP 1 Score: 634.4 bits (1635), Expect = 1.1e-180
Identity = 306/524 (58.40%), Postives = 398/524 (75.95%), Query Frame = 1
Query: 34 NPLQKFAASLLDAVESSLFVD------KLPKTIDPAVQISGNFSPVPECSVRHDLEIIGN 93
N Q+ AA++L+AVE +L + LPKT DPAVQI+GNF+PV E HDL + G
Sbjct: 96 NLFQRAAAAVLEAVEDNLIQNLLESGHPLPKTADPAVQIAGNFAPVGEQKPHHDLPVDGR 155
Query: 94 LPACLRGIYLRNGANPMHAPTGGHHLFDGDGMIHAVTFHSGNKASYSCRFTRTNRLQQEA 153
+P + G+YLRNGANP+ P GHH FDGDGM+HAV +G +ASY+CRFT T RL+QE
Sbjct: 156 IPPLINGVYLRNGANPLFEPVAGHHFFDGDGMVHAVHLRNG-RASYACRFTETERLKQER 215
Query: 154 ALGRLVFPKPIGELHGHQGLARLAIFLARAGIGLIDGSKGTGVANAGLVYFNGRLLALSE 213
A+GR +FPK IGELHGH G+ARL +F AR +GLID S+GTGVANAGL+YFN RLLA+SE
Sbjct: 216 AVGRAIFPKAIGELHGHSGIARLLLFYARGLLGLIDHSRGTGVANAGLIYFNNRLLAMSE 275
Query: 214 DDLPYHVQIKDDGDLETIGRFNFNGQINCPMIAHPKVDPISGDLHGLSYNMIKKPYLKYL 273
DDLPYHV+IK +GDLET GR++F+GQ+ MIAHPK+DP + + LSY++IKKPYLKY
Sbjct: 276 DDLPYHVRIKPNGDLETAGRYDFDGQLTTTMIAHPKLDPETREFFALSYDVIKKPYLKYF 335
Query: 274 RFDRFGKKSRDVDITLREPTMIHDFAITENHVVIPDHQVVFKLLEMVRGGSPVVFDPKKT 333
RF G+KS DV+I L +PTM+HDFAIT+N V+IPD QVVFKL EM+ GGSPVV+D +K
Sbjct: 336 RFSPCGEKSPDVEIPLPQPTMMHDFAITKNFVIIPDQQVVFKLQEMICGGSPVVYDKEKI 395
Query: 334 SRFGILPKSGVDEKGIVWIEVPNCFCFHLWNAWEETGGNDEKSIVVVGSCMNPPDSIFND 393
+RFG+LPK +D + WI+VP+CFCFHLWN+WEE + + +VV+GSCM PPDSIFN+
Sbjct: 396 ARFGVLPKYAIDASEMQWIDVPDCFCFHLWNSWEEP---ETEEVVVIGSCMTPPDSIFNE 455
Query: 394 RDQPLRIELTEIRMDVKSRKVTRRVF----GSGMNLEAGQMNRGLVGRKTRFVYMAIADP 453
++ L+ LTEIR+++++ K TRR S +NLEAG +NR +GR+TRF Y+AIA+P
Sbjct: 456 SEENLQSVLTEIRLNLRTGKSTRRPILRPGNSQINLEAGMVNRNRLGRRTRFAYLAIAEP 515
Query: 454 WPKCSGIAKVDLETGKVKKFLYGEGRYGGEPFYVPENGNWGGDNEKEDGGYIVGFVRDEK 513
WPK SG AKVDL +G++++F YG+G YGGEP++VP G ++EDGGY++ FV DE+
Sbjct: 516 WPKVSGFAKVDLASGEIQRFEYGDGGYGGEPYFVPREG-----CDREDGGYVLAFVHDER 575
Query: 514 RERSEVVVVKAAEMEEVAAVRLPVRVPYGFHGTFVSEEELNGQA 548
SE++++ AA+M AAVRLP RVPYGF+GTFVS EL+ QA
Sbjct: 576 EGSSELLIMNAADMRLEAAVRLPSRVPYGFYGTFVSATELHSQA 610
BLAST of Cucsa.194320 vs. TrEMBL
Match:
A0A0A0LIE2_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_2G160620 PE=4 SV=1)
HSP 1 Score: 1114.8 bits (2882), Expect = 0.0e+00
Identity = 549/568 (96.65%), Postives = 549/568 (96.65%), Query Frame = 1
Query: 1 MQISLQSPPPPLTLCRSSSTIETPPIRKLK-------------------RRLNPLQKFAA 60
MQISLQSPPPPLTLCRSSSTIETPPIRKLK RRLNPLQKFAA
Sbjct: 1 MQISLQSPPPPLTLCRSSSTIETPPIRKLKVITPPRPPLSLPKKVSPPQRRLNPLQKFAA 60
Query: 61 SLLDAVESSLFVDKLPKTIDPAVQISGNFSPVPECSVRHDLEIIGNLPACLRGIYLRNGA 120
SLLDAVESSLFVDKLPKTIDPAVQISGNFSPVPECSVRHDLEIIGNLPACLRGIYLRNGA
Sbjct: 61 SLLDAVESSLFVDKLPKTIDPAVQISGNFSPVPECSVRHDLEIIGNLPACLRGIYLRNGA 120
Query: 121 NPMHAPTGGHHLFDGDGMIHAVTFHSGNKASYSCRFTRTNRLQQEAALGRLVFPKPIGEL 180
NPMHAPTGGHHLFDGDGMIHAVTFHSGNKASYSCRFTRTNRLQQEAALGRLVFPKPIGEL
Sbjct: 121 NPMHAPTGGHHLFDGDGMIHAVTFHSGNKASYSCRFTRTNRLQQEAALGRLVFPKPIGEL 180
Query: 181 HGHQGLARLAIFLARAGIGLIDGSKGTGVANAGLVYFNGRLLALSEDDLPYHVQIKDDGD 240
HGHQGLARLAIFLARAGIGLIDGSKGTGVANAGLVYFNGRLLALSEDDLPYHVQIKDDGD
Sbjct: 181 HGHQGLARLAIFLARAGIGLIDGSKGTGVANAGLVYFNGRLLALSEDDLPYHVQIKDDGD 240
Query: 241 LETIGRFNFNGQINCPMIAHPKVDPISGDLHGLSYNMIKKPYLKYLRFDRFGKKSRDVDI 300
LETIGRFNFNGQINCPMIAHPKVDPISGDLHGLSYNMIKKPYLKYLRFDRFGKKSRDVDI
Sbjct: 241 LETIGRFNFNGQINCPMIAHPKVDPISGDLHGLSYNMIKKPYLKYLRFDRFGKKSRDVDI 300
Query: 301 TLREPTMIHDFAITENHVVIPDHQVVFKLLEMVRGGSPVVFDPKKTSRFGILPKSGVDEK 360
TLREPTMIHDFAITENHVVIPDHQVVFKLLEMVRGGSPVVFDPKKTSRFGILPKSGVDEK
Sbjct: 301 TLREPTMIHDFAITENHVVIPDHQVVFKLLEMVRGGSPVVFDPKKTSRFGILPKSGVDEK 360
Query: 361 GIVWIEVPNCFCFHLWNAWEETGGNDEKSIVVVGSCMNPPDSIFNDRDQPLRIELTEIRM 420
GIVWIEVPNCFCFHLWNAWEETGGNDEKSIVVVGSCMNPPDSIFNDRDQPLRIELTEIRM
Sbjct: 361 GIVWIEVPNCFCFHLWNAWEETGGNDEKSIVVVGSCMNPPDSIFNDRDQPLRIELTEIRM 420
Query: 421 DVKSRKVTRRVFGSGMNLEAGQMNRGLVGRKTRFVYMAIADPWPKCSGIAKVDLETGKVK 480
DVKSRKVTRRVFGSGMNLEAGQMNRGLVGRKTRFVYMAIADPWPKCSGIAKVDLETGKVK
Sbjct: 421 DVKSRKVTRRVFGSGMNLEAGQMNRGLVGRKTRFVYMAIADPWPKCSGIAKVDLETGKVK 480
Query: 481 KFLYGEGRYGGEPFYVPENGNWGGDNEKEDGGYIVGFVRDEKRERSEVVVVKAAEMEEVA 540
KFLYGEGRYGGEPFYVPENGNWGGDNEKEDGGYIVGFVRDEKRERSEVVVVKAAEMEEVA
Sbjct: 481 KFLYGEGRYGGEPFYVPENGNWGGDNEKEDGGYIVGFVRDEKRERSEVVVVKAAEMEEVA 540
Query: 541 AVRLPVRVPYGFHGTFVSEEELNGQARN 550
AVRLPVRVPYGFHGTFVSEEELNGQARN
Sbjct: 541 AVRLPVRVPYGFHGTFVSEEELNGQARN 568
BLAST of Cucsa.194320 vs. TrEMBL
Match:
D5G3I2_CITLA (9-cis epoxycarotenoid dioxygenase (Fragment) OS=Citrullus lanatus GN=NCED1 PE=2 SV=1)
HSP 1 Score: 818.5 bits (2113), Expect = 4.8e-234
Identity = 392/422 (92.89%), Postives = 413/422 (97.87%), Query Frame = 1
Query: 113 LFDGDGMIHAVTFHSGNKASYSCRFTRTNRLQQEAALGRLVFPKPIGELHGHQGLARLAI 172
LFDGDGMIHAVTFH GN+ASYSCRFTRT+RLQQEA+LGR VFPKPIGELHGHQGLARLAI
Sbjct: 1 LFDGDGMIHAVTFHCGNRASYSCRFTRTSRLQQEASLGRPVFPKPIGELHGHQGLARLAI 60
Query: 173 FLARAGIGLIDGSKGTGVANAGLVYFNGRLLALSEDDLPYHVQIKDDGDLETIGRFNFNG 232
FLARAGIGLIDGSKGTGVANAGLVYFNGRLLALSEDDLPYHV+IKDDGDLETIGRFNFNG
Sbjct: 61 FLARAGIGLIDGSKGTGVANAGLVYFNGRLLALSEDDLPYHVKIKDDGDLETIGRFNFNG 120
Query: 233 QINCPMIAHPKVDPISGDLHGLSYNMIKKPYLKYLRFDRFGKKSRDVDITLREPTMIHDF 292
QINCP+IAHPKVDPISGDLHGLSYNMIKKPYLKYLRFDRFGKKSRDVDI+LREPTMIHDF
Sbjct: 121 QINCPIIAHPKVDPISGDLHGLSYNMIKKPYLKYLRFDRFGKKSRDVDISLREPTMIHDF 180
Query: 293 AITENHVVIPDHQVVFKLLEMVRGGSPVVFDPKKTSRFGILPKSGVDEKGIVWIEVPNCF 352
AITENHVVIPDHQVVFKL EM+RGGSPVVFDPKKTSRFGILPKSGVDEKGIVWIEVP+CF
Sbjct: 181 AITENHVVIPDHQVVFKLSEMIRGGSPVVFDPKKTSRFGILPKSGVDEKGIVWIEVPDCF 240
Query: 353 CFHLWNAWEETGGNDEKSIVVVGSCMNPPDSIFNDRDQPLRIELTEIRMDVKSRKVTRRV 412
CFHLWNAWEETG NDE+++VVVGSCMNPPDSIFNDRDQPLRIELTEIRMDV++RKVTRRV
Sbjct: 241 CFHLWNAWEETGDNDEETVVVVGSCMNPPDSIFNDRDQPLRIELTEIRMDVRNRKVTRRV 300
Query: 413 FGSGMNLEAGQMNRGLVGRKTRFVYMAIADPWPKCSGIAKVDLETGKVKKFLYGEGRYGG 472
FGSG+NLEAGQ+NR LVG++TRFVYMAIA+PWPKCSGIAKVDLETGKVKKFLYG+GRYGG
Sbjct: 301 FGSGLNLEAGQVNRRLVGQRTRFVYMAIAEPWPKCSGIAKVDLETGKVKKFLYGDGRYGG 360
Query: 473 EPFYVPENGNWGGDNEKEDGGYIVGFVRDEKRERSEVVVVKAAEMEEVAAVRLPVRVPYG 532
EPFYV ENGN GGD+E EDGG+IVGFVRDEKRERSEVVVVKAAEMEEVAAVRLP RVPYG
Sbjct: 361 EPFYVSENGNHGGDHENEDGGFIVGFVRDEKRERSEVVVVKAAEMEEVAAVRLPARVPYG 420
Query: 533 FH 535
FH
Sbjct: 421 FH 422
BLAST of Cucsa.194320 vs. TrEMBL
Match:
B9RWM0_RICCO (9-cis-epoxycarotenoid dioxygenase, putative OS=Ricinus communis GN=RCOM_1021470 PE=4 SV=1)
HSP 1 Score: 783.5 bits (2022), Expect = 1.7e-223
Identity = 381/570 (66.84%), Postives = 459/570 (80.53%), Query Frame = 1
Query: 2 QISLQSPPPPLTLCRSSS-----TIETPPIRKLKRRLNPLQKFAASLLDAVESSLFVD-- 61
++ L PPPPL+ S T TP LNPLQK AA LD +ESSL V
Sbjct: 47 KLPLSLPPPPLSEPAPPSLEPGPTFTTPRETDHPIALNPLQKLAALALDKIESSLLVPFE 106
Query: 62 ---KLPKTIDPAVQISGNFSPVPECSVRHDLEIIGNLPACLRGIYLRNGANPMHAPTGGH 121
LP+TIDP VQISGNF+PV EC V H LE++G++P LRG+YLRNGANP++APTGGH
Sbjct: 107 RNHLLPRTIDPTVQISGNFAPVLECPVHHGLEVVGHIPNSLRGVYLRNGANPVYAPTGGH 166
Query: 122 HLFDGDGMIHAVTFHSGNKASYSCRFTRTNRLQQEAALGRLVFPKPIGELHGHQGLARLA 181
HLFDGDGM+HAVT N+ASYSCRFTRT+RL+QEAALGR VFPKPIGELHGH G ARL
Sbjct: 167 HLFDGDGMVHAVTLGYENRASYSCRFTRTSRLEQEAALGRSVFPKPIGELHGHLGFARLL 226
Query: 182 IFLARAGIGLIDGSKGTGVANAGLVYFNGRLLALSEDDLPYHVQIKDDGDLETIGRFNFN 241
IF+ RAGIGL+DGS+GTGVANAGLVYFNGRLLA+SEDDLPYHV+IK DGDLETI RF+F+
Sbjct: 227 IFMVRAGIGLVDGSRGTGVANAGLVYFNGRLLAMSEDDLPYHVKIKGDGDLETIKRFSFD 286
Query: 242 GQINCPMIAHPKVDPISGDLHGLSYNMIKKPYLKYLRFDRFGKKSRDVDITLREPTMIHD 301
Q++CPMIAHPKVDP++G+LH LSYN+++KPYLKY +FD++GKKSRD+DITL +PTMIHD
Sbjct: 287 DQLDCPMIAHPKVDPVTGELHALSYNVVQKPYLKYFKFDKYGKKSRDLDITLHQPTMIHD 346
Query: 302 FAITENHVVIPDHQVVFKLLEMVRGGSPVVFDPKKTSRFGILPKSGVDEKGIVWIEVPNC 361
FA+T N VVIPDHQVVFKL EM+RGGSPV++D KTSRFGIL K+ V+E GI WIEVPNC
Sbjct: 347 FAVTRNFVVIPDHQVVFKLSEMIRGGSPVIYDKNKTSRFGILSKNEVNESGIQWIEVPNC 406
Query: 362 FCFHLWNAWEETGGNDEKSIVVVGSCMNPPDSIFNDRDQPLRIELTEIRMDVKSRKVTRR 421
FCFHLWN+WEE N +K IVV+GSCMNPPDSIFN+ + ++ EL+EIR+++++R+ TR+
Sbjct: 407 FCFHLWNSWEEISTNGDKIIVVIGSCMNPPDSIFNESETAIQSELSEIRLNMRTRESTRK 466
Query: 422 VFGSGMNLEAGQMNRGLVGRKTRFVYMAIADPWPKCSGIAKVDLETGKVKKFLYGEGRYG 481
GMNLEAGQ+NR VG+KTR+VY+AIA+PWPKCSGIAKVDLET +V KF+YG+GRYG
Sbjct: 467 AIVRGMNLEAGQVNRKFVGQKTRYVYLAIAEPWPKCSGIAKVDLETKEVTKFMYGKGRYG 526
Query: 482 GEPFYVPEN------------GNWGGD--NEKEDGGYIVGFVRDEKRERSEVVVVKAAEM 541
GEPF+V +N G G+ N++E GYI+GFVRDE +ERSE+V+V A M
Sbjct: 527 GEPFFVAKNREGEEEEDDDDDGKKIGNVINDEEGEGYIMGFVRDEIKERSELVIVNATGM 586
Query: 542 EEVAAVRLPVRVPYGFHGTFVSEEELNGQA 548
E+VA+V LP RVPYGFHGTFVSE+EL GQA
Sbjct: 587 EQVASVSLPTRVPYGFHGTFVSEDELKGQA 616
BLAST of Cucsa.194320 vs. TrEMBL
Match:
A0A061EL25_THECC (Nine-cis-epoxycarotenoid dioxygenase 6 OS=Theobroma cacao GN=TCM_020388 PE=4 SV=1)
HSP 1 Score: 780.4 bits (2014), Expect = 1.4e-222
Identity = 380/558 (68.10%), Postives = 457/558 (81.90%), Query Frame = 1
Query: 8 PPPPLTLCRSSSTIETPPIRKLKR-----------RLNPLQKFAASLLDAVESSLF--VD 67
PPPP RS E PI K +R LNPLQK AA LD +E+S+ ++
Sbjct: 50 PPPP---SRSPLYPEVEPIVKPERVLVPHEPNYPVHLNPLQKLAALALDQIETSVIKPLE 109
Query: 68 K---LPKTIDPAVQISGNFSPVPECSVRHDLEIIGNLPACLRGIYLRNGANPMHAPTGGH 127
K LPKT+DPAVQISGNF+PV ECSV+H LE++G +PACLRG+Y+RNGANPM AP+GGH
Sbjct: 110 KNHDLPKTVDPAVQISGNFAPVQECSVQHWLEVVGQIPACLRGVYVRNGANPMFAPSGGH 169
Query: 128 HLFDGDGMIHAVTFHSGNKASYSCRFTRTNRLQQEAALGRLVFPKPIGELHGHQGLARLA 187
HLFDGDGMIHAV+ GN+ASYSCR+TRT+RL QEA LGR +FPKPIGELHGH GLARLA
Sbjct: 170 HLFDGDGMIHAVSLGRGNEASYSCRYTRTSRLMQEARLGRSIFPKPIGELHGHLGLARLA 229
Query: 188 IFLARAGIGLIDGSKGTGVANAGLVYFNGRLLALSEDDLPYHVQIKDDGDLETIGRFNFN 247
+F+AR G+GL+DGS GTGVANAGLVYFNGRLLA+SEDDLPYHV+I D DLET+GRFNF+
Sbjct: 230 LFMARDGVGLVDGSHGTGVANAGLVYFNGRLLAMSEDDLPYHVKINADADLETMGRFNFD 289
Query: 248 GQINCPMIAHPKVDPISGDLHGLSYNMIKKPYLKYLRFDRFGKKSRDVDITLREPTMIHD 307
QI+CP+IAHPKVDP++G+LH L+YN++KKPYLKY +FD+FGKKSRD+ +++ + TMIHD
Sbjct: 290 DQIDCPLIAHPKVDPVTGELHTLNYNVLKKPYLKYFKFDKFGKKSRDLHVSIEQATMIHD 349
Query: 308 FAITENHVVIPDHQVVFKLLEMVRGGSPVVFDPKKTSRFGILPKSGVDEKGIVWIEVPNC 367
FAITEN VVIPDHQVVFKL EM+RGGSPV++D KKTSRFGIL ++ VD I WIEVPNC
Sbjct: 350 FAITENFVVIPDHQVVFKLSEMIRGGSPVLYDKKKTSRFGILQQNDVDGSNIQWIEVPNC 409
Query: 368 FCFHLWNAWEETGGNDEKSIVVVGSCMNPPDSIFNDRDQPLRIELTEIRMDVKSRKVTRR 427
FCFHLWNAWEE N +K IVV+GSCM+PPDS+FN+ P R EL+EIR+++++ + T+R
Sbjct: 410 FCFHLWNAWEEHSDNGDKIIVVIGSCMSPPDSVFNESKDPFRSELSEIRLNLRTGESTQR 469
Query: 428 VFGSGMNLEAGQMNRGLVGRKTRFVYMAIADPWPKCSGIAKVDLETGKVKKFLYGEGRYG 487
V SG NLEAGQ+NR GRKTRFVY+AIA+PWPKCSGI KVDLETG+V KF+YG R+G
Sbjct: 470 VVISGTNLEAGQVNRQYQGRKTRFVYLAIAEPWPKCSGITKVDLETGEVTKFMYGACRFG 529
Query: 488 GEPFYVPE-NGNWGGD--NEKEDGGYIVGFVRDEKRERSEVVVVKAAEMEEVAAVRLPVR 547
GEPF+VPE GG+ NE ED GYI+GFVRDEK+E SE+V+VKA ME+VA++RLP R
Sbjct: 530 GEPFFVPEKKQKHGGNIANEAEDEGYIMGFVRDEKKEISELVIVKALSMEQVASIRLPTR 589
BLAST of Cucsa.194320 vs. TrEMBL
Match:
V4S0R4_9ROSI (Uncharacterized protein OS=Citrus clementina GN=CICLE_v10006710mg PE=4 SV=1)
HSP 1 Score: 774.6 bits (1999), Expect = 7.9e-221
Identity = 367/549 (66.85%), Postives = 448/549 (81.60%), Query Frame = 1
Query: 5 LQSPPPPLTLCRSSSTIETPPIRKLKRRLNPLQKFAASLLDAVESSL--FVDK---LPKT 64
+ +PPPP T P K LNPLQK AAS LD +E SL F +K LPKT
Sbjct: 48 MPTPPPP-----DPVTFLEPVRPKYPHHLNPLQKLAASALDKIEDSLIGFFEKGKSLPKT 107
Query: 65 IDPAVQISGNFSPVPECSVRHDLEIIGNLPACLRGIYLRNGANPMHAPTGGHHLFDGDGM 124
+DP VQISGNF+PV EC V H L+++G +P CLRG+Y+RNGANPM P+GGHHLFDGDGM
Sbjct: 108 VDPTVQISGNFAPVQECPVHHGLQVVGRIPDCLRGVYVRNGANPMFVPSGGHHLFDGDGM 167
Query: 125 IHAVTFHSGNKASYSCRFTRTNRLQQEAALGRLVFPKPIGELHGHQGLARLAIFLARAGI 184
IHAVT N+ASYSCR+T+T+RL QEA G+ VFPKPIGELHGH GLARL +F+ARAG
Sbjct: 168 IHAVTLGHANQASYSCRYTQTSRLVQEAKYGKPVFPKPIGELHGHLGLARLGLFMARAGA 227
Query: 185 GLIDGSKGTGVANAGLVYFNGRLLALSEDDLPYHVQIKDDGDLETIGRFNFNGQINCPMI 244
GL+DGS+G G ANAGLVYFNG LLA+SEDDLPY V+IK DGDLETIGRF+F+ Q++CPMI
Sbjct: 228 GLVDGSRGMGAANAGLVYFNGSLLAMSEDDLPYRVKIKPDGDLETIGRFSFDDQLDCPMI 287
Query: 245 AHPKVDPISGDLHGLSYNMIKKPYLKYLRF--DRFGKKSRDVDITLREPTMIHDFAITEN 304
AHPKVDP++GDLH LSYN+++KPYLKY RF GKKSRDVD+TL +PTMIHDFAITEN
Sbjct: 288 AHPKVDPVTGDLHALSYNVVRKPYLKYFRFGGSNNGKKSRDVDVTLDQPTMIHDFAITEN 347
Query: 305 HVVIPDHQVVFKLLEMVRGGSPVVFDPKKTSRFGILPKSGVDEKGIVWIEVPNCFCFHLW 364
V+IPDHQVVFKL EM+RGGSPV++D KTSRFG+L K+ DE GI WI+VPNCFCFHLW
Sbjct: 348 CVIIPDHQVVFKLSEMIRGGSPVIYDKHKTSRFGVLSKNDADESGIQWIDVPNCFCFHLW 407
Query: 365 NAWEETGGNDEKSIVVVGSCMNPPDSIFNDRDQPLRIELTEIRMDVKSRKVTRRVFGSGM 424
NAWEE + ++++VV+GSCM+PPDS+FN++ P+R EL+EIR+++K+ K TRRV SGM
Sbjct: 408 NAWEENNDDGDRNVVVIGSCMDPPDSVFNEQQDPVRSELSEIRLNLKTGKSTRRVIVSGM 467
Query: 425 NLEAGQMNRGLVGRKTRFVYMAIADPWPKCSGIAKVDLETGKVKKFLYGEGRYGGEPFYV 484
NLEAGQ+NR L+G+KTR+VY+AIADPWPKCSGIAKVDL TG+V K +YGE R+GGEP +V
Sbjct: 468 NLEAGQVNRALLGQKTRYVYLAIADPWPKCSGIAKVDLGTGEVTKLMYGEHRFGGEPMFV 527
Query: 485 PENGNWGGDNEKEDGGYIVGFVRDEKRERSEVVVVKAAEMEEVAAVRLPVRVPYGFHGTF 544
P+N G ED G+I+GFVRDE+RE+SE+ +VKA+ ME+VA+V+LP RVPYGFHGTF
Sbjct: 528 PQNSKNAGSG-SEDEGFIMGFVRDERREQSEMAIVKASSMEQVASVKLPRRVPYGFHGTF 587
Query: 545 VSEEELNGQ 547
+SE+EL+GQ
Sbjct: 588 ISEQELSGQ 590
BLAST of Cucsa.194320 vs. TAIR10
Match:
AT3G24220.1 (AT3G24220.1 nine-cis-epoxycarotenoid dioxygenase 6)
HSP 1 Score: 696.8 bits (1797), Expect = 1.1e-200
Identity = 334/540 (61.85%), Postives = 428/540 (79.26%), Query Frame = 1
Query: 13 TLCRSSSTIETP-PIRKLKRRLNPLQKFAASLLDAVESSLFVDK-----LPKTIDPAVQI 72
TL +S + +P ++ LN LQK AA++LD +ESS+ + LPK DPAVQ+
Sbjct: 42 TLPDLTSPVPSPVKLKPTYPNLNLLQKLAATMLDKIESSIVIPMEQNRPLPKPTDPAVQL 101
Query: 73 SGNFSPVPECSVRHDLEIIGNLPACLRGIYLRNGANPMHAPTGGHHLFDGDGMIHAVTFH 132
SGNF+PV EC V++ LE++G +P+CL+G+Y+RNGANPM P GHHLFDGDGMIHAV+
Sbjct: 102 SGNFAPVNECPVQNGLEVVGQIPSCLKGVYIRNGANPMFPPLAGHHLFDGDGMIHAVSIG 161
Query: 133 SGNKASYSCRFTRTNRLQQEAALGRLVFPKPIGELHGHQGLARLAIFLARAGIGLIDGSK 192
N+ SYSCR+T+TNRL QE ALGR VFPKPIGELHGH GLARLA+F ARAGIGL+DG++
Sbjct: 162 FDNQVSYSCRYTKTNRLVQETALGRSVFPKPIGELHGHSGLARLALFTARAGIGLVDGTR 221
Query: 193 GTGVANAGLVYFNGRLLALSEDDLPYHVQIKDDGDLETIGRFNFNGQINCPMIAHPKVDP 252
G GVANAG+V+FNGRLLA+SEDDLPY V+I GDLETIGRF F+ QI+ +IAHPKVD
Sbjct: 222 GMGVANAGVVFFNGRLLAMSEDDLPYQVKIDGQGDLETIGRFGFDDQIDSSVIAHPKVDA 281
Query: 253 ISGDLHGLSYNMIKKPYLKYLRFDRFGKKSRDVDITLREPTMIHDFAITENHVVIPDHQV 312
+GDLH LSYN++KKP+L+YL+F+ GKK+RDV+ITL EPTMIHDFAITEN VVIPD Q+
Sbjct: 282 TTGDLHTLSYNVLKKPHLRYLKFNTCGKKTRDVEITLPEPTMIHDFAITENFVVIPDQQM 341
Query: 313 VFKLLEMVRGGSPVVFDPKKTSRFGILPKSGVDEKGIVWIEVPNCFCFHLWNAWEETGGN 372
VFKL EM+RGGSPV++ +K +RFG+L K + I W++VP+CFCFHLWNAWEE
Sbjct: 342 VFKLSEMIRGGSPVIYVKEKMARFGVLSKQDLTGSDINWVDVPDCFCFHLWNAWEERTEE 401
Query: 373 DEKSIVVVGSCMNPPDSIFNDRDQPLRIELTEIRMDVKSRKVTRRVFGSGMNLEAGQMNR 432
+ IVV+GSCM+PPD+IF++ +P R+EL+EIR+++++++ R+V +G+NLEAG +NR
Sbjct: 402 GDPVIVVIGSCMSPPDTIFSESGEPTRVELSEIRLNMRTKESNRKVIVTGVNLEAGHINR 461
Query: 433 GLVGRKTRFVYMAIADPWPKCSGIAKVDLETGKVKKFLYGEGRYGGEPFYVPENGNWGGD 492
VGRK++FVY+AIADPWPKCSGIAKVD++ G V +F YG R+GGEP +VPE G+
Sbjct: 462 SYVGRKSQFVYIAIADPWPKCSGIAKVDIQNGTVSEFNYGPSRFGGEPCFVPE-----GE 521
Query: 493 NEKEDGGYIVGFVRDEKRERSEVVVVKAAEMEEVAAVRLPVRVPYGFHGTFVSEEELNGQ 547
E ED GY++GFVRDE+++ SE VVV A +M++VAAVRLP RVPYGFHGTFVSE +L Q
Sbjct: 522 GE-EDKGYVMGFVRDEEKDESEFVVVDATDMKQVAAVRLPERVPYGFHGTFVSENQLKEQ 575
BLAST of Cucsa.194320 vs. TAIR10
Match:
AT1G78390.1 (AT1G78390.1 nine-cis-epoxycarotenoid dioxygenase 9)
HSP 1 Score: 647.1 bits (1668), Expect = 9.6e-186
Identity = 320/553 (57.87%), Postives = 408/553 (73.78%), Query Frame = 1
Query: 4 SLQSP---PPPLTLCRSSSTIETPPIRKLKRRLNPLQKFAASLLDAVESSLFVDKL---- 63
SLQSP PP + T P +LNP+Q+ AA +LDAVE+++ +
Sbjct: 113 SLQSPTFKPPSWKKLCNDVTNLIPKTTNQNPKLNPVQRTAAMVLDAVENAMISHERRRHP 172
Query: 64 -PKTIDPAVQISGNFSPVPECSVRHDLEIIGNLPACLRGIYLRNGANPMHAPTGGHHLFD 123
PKT DPAVQI+GNF PVPE V H+L + G +P C++G+Y+RNGANP+H P GHHLFD
Sbjct: 173 HPKTADPAVQIAGNFFPVPEKPVVHNLPVTGTVPECIQGVYVRNGANPLHKPVSGHHLFD 232
Query: 124 GDGMIHAVTFHSGNKASYSCRFTRTNRLQQEAALGRLVFPKPIGELHGHQGLARLAIFLA 183
GDGM+HAV F +G+ SY+CRFT TNRL QE GR VFPK IGELHGH G+A+L +F
Sbjct: 233 GDGMVHAVRFDNGS-VSYACRFTETNRLVQERECGRPVFPKAIGELHGHLGIAKLMLFNT 292
Query: 184 RAGIGLIDGSKGTGVANAGLVYFNGRLLALSEDDLPYHVQIKDDGDLETIGRFNFNGQIN 243
R GL+D + G GVANAGLVYFNG LLA+SEDDLPYHV++ GDLET GR++F+GQ+
Sbjct: 293 RGLFGLVDPTGGLGVANAGLVYFNGHLLAMSEDDLPYHVKVTQTGDLETSGRYDFDGQLK 352
Query: 244 CPMIAHPKVDPISGDLHGLSYNMIKKPYLKYLRFDRFGKKSRDVDITLREPTMIHDFAIT 303
MIAHPK+DP + +L LSY+++ KPYLKY RF G+KS DV+I L +PTMIHDFAIT
Sbjct: 353 STMIAHPKIDPETRELFALSYDVVSKPYLKYFRFTSDGEKSPDVEIPLDQPTMIHDFAIT 412
Query: 304 ENHVVIPDHQVVFKLLEMVRGGSPVVFDPKKTSRFGILPKSGVDEKGIVWIEVPNCFCFH 363
EN VVIPD QVVF+L EM+RGGSPVV+D KK SRFGIL K+ D I WIEVP+CFCFH
Sbjct: 413 ENFVVIPDQQVVFRLPEMIRGGSPVVYDEKKKSRFGILNKNAKDASSIQWIEVPDCFCFH 472
Query: 364 LWNAWEETGGNDEKSIVVVGSCMNPPDSIFNDRDQPLRIELTEIRMDVKSRKVTRR-VFG 423
LWN+WEE + +VV+GSCM PPDSIFN+ D+ L+ L+EIR+++K+ + TRR V
Sbjct: 473 LWNSWEEP---ETDEVVVIGSCMTPPDSIFNEHDETLQSVLSEIRLNLKTGESTRRPVIS 532
Query: 424 SGMNLEAGQMNRGLVGRKTRFVYMAIADPWPKCSGIAKVDLETGKVKKFLYGEGRYGGEP 483
+NLEAG +NR L+GRKTR+ Y+A+ +PWPK SG AKVDL TG+++K++YGEG+YGGEP
Sbjct: 533 EQVNLEAGMVNRNLLGRKTRYAYLALTEPWPKVSGFAKVDLSTGEIRKYIYGEGKYGGEP 592
Query: 484 FYVPENGNWGGDNEKEDGGYIVGFVRDEKRERSEVVVVKAAEMEEVAAVRLPVRVPYGFH 543
++P GD E EDGGYI+ FV DE++ +SE+ ++ A M+ A V LP RVPYGFH
Sbjct: 593 LFLP-----SGDGE-EDGGYIMVFVHDEEKVKSELQLINAVNMKLEATVTLPSRVPYGFH 652
Query: 544 GTFVSEEELNGQA 548
GTF+S+E+L+ QA
Sbjct: 653 GTFISKEDLSKQA 655
BLAST of Cucsa.194320 vs. TAIR10
Match:
AT3G14440.1 (AT3G14440.1 nine-cis-epoxycarotenoid dioxygenase 3)
HSP 1 Score: 634.8 bits (1636), Expect = 4.9e-182
Identity = 316/555 (56.94%), Postives = 403/555 (72.61%), Query Frame = 1
Query: 4 SLQSPPP---PLTLCRSSSTIETPPIRKLK-RRLNPLQKFAASLLDAVESSLF----VDK 63
+L +PP P S + + P ++ +++N Q+ AA+ LDA E L +
Sbjct: 53 ALHTPPALHFPKQSSNSPAIVVKPKAKESNTKQMNLFQRAAAAALDAAEGFLVSHEKLHP 112
Query: 64 LPKTIDPAVQISGNFSPVPECSVRHDLEIIGNLPACLRGIYLRNGANPMHAPTGGHHLFD 123
LPKT DP+VQI+GNF+PV E VR +L ++G LP ++G+Y+RNGANP+H P GHH FD
Sbjct: 113 LPKTADPSVQIAGNFAPVNEQPVRRNLPVVGKLPDSIKGVYVRNGANPLHEPVTGHHFFD 172
Query: 124 GDGMIHAVTFHSGNKASYSCRFTRTNRLQQEAALGRLVFPKPIGELHGHQGLARLAIFLA 183
GDGM+HAV F G+ ASY+CRFT+TNR QE LGR VFPK IGELHGH G+ARL +F A
Sbjct: 173 GDGMVHAVKFEHGS-ASYACRFTQTNRFVQERQLGRPVFPKAIGELHGHTGIARLMLFYA 232
Query: 184 RAGIGLIDGSKGTGVANAGLVYFNGRLLALSEDDLPYHVQIKDDGDLETIGRFNFNGQIN 243
RA G++D + GTGVANAGLVYFNGRLLA+SEDDLPY VQI +GDL+T+GRF+F+GQ+
Sbjct: 233 RAAAGIVDPAHGTGVANAGLVYFNGRLLAMSEDDLPYQVQITPNGDLKTVGRFDFDGQLE 292
Query: 244 CPMIAHPKVDPISGDLHGLSYNMIKKPYLKYLRFDRFGKKSRDVDITLREPTMIHDFAIT 303
MIAHPKVDP SG+L LSY+++ KPYLKY RF G KS DV+I L +PTM+HDFAIT
Sbjct: 293 STMIAHPKVDPESGELFALSYDVVSKPYLKYFRFSPDGTKSPDVEIQLDQPTMMHDFAIT 352
Query: 304 ENHVVIPDHQVVFKLLEMVRGGSPVVFDPKKTSRFGILPKSGVDEKGIVWIEVPNCFCFH 363
EN VV+PD QVVFKL EM+RGGSPVV+D K +RFGIL K D I WI+ P+CFCFH
Sbjct: 353 ENFVVVPDQQVVFKLPEMIRGGSPVVYDKNKVARFGILDKYAEDSSNIKWIDAPDCFCFH 412
Query: 364 LWNAWEETGGNDEKSIVVVGSCMNPPDSIFNDRDQPLRIELTEIRMDVKSRKVTRRVFGS 423
LWNAWEE + +VV+GSCM PPDSIFN+ D+ L+ L+EIR+++K+ + TRR S
Sbjct: 413 LWNAWEEP---ETDEVVVIGSCMTPPDSIFNESDENLKSVLSEIRLNLKTGESTRRPIIS 472
Query: 424 G----MNLEAGQMNRGLVGRKTRFVYMAIADPWPKCSGIAKVDLETGKVKKFLYGEGRYG 483
+NLEAG +NR ++GRKT+F Y+A+A+PWPK SG AKVDL TG+VKK LYG+ RYG
Sbjct: 473 NEDQQVNLEAGMVNRNMLGRKTKFAYLALAEPWPKVSGFAKVDLTTGEVKKHLYGDNRYG 532
Query: 484 GEPFYVPENGNWGGDNEKEDGGYIVGFVRDEKRERSEVVVVKAAEMEEVAAVRLPVRVPY 543
GEP ++P G+ +ED GYI+ FV DEK +SE+ +V A +E A V+LP RVPY
Sbjct: 533 GEPLFLP------GEGGEEDEGYILCFVHDEKTWKSELQIVNAVSLEVEATVKLPSRVPY 592
Query: 544 GFHGTFVSEEELNGQ 547
GFHGTF+ ++L Q
Sbjct: 593 GFHGTFIGADDLAKQ 597
BLAST of Cucsa.194320 vs. TAIR10
Match:
AT4G18350.1 (AT4G18350.1 nine-cis-epoxycarotenoid dioxygenase 2)
HSP 1 Score: 619.4 bits (1596), Expect = 2.1e-177
Identity = 308/533 (57.79%), Postives = 386/533 (72.42%), Query Frame = 1
Query: 20 TIETPPIRKLKRRLNPLQKFAASLLDAVESSLFVDK----LPKTIDPAVQISGNFSPVPE 79
T TPP RLN QK AA +DA E +L + LPKT DP VQI+GN+SPVPE
Sbjct: 57 TTATPPQHN-PLRLNIFQKAAAIAIDAAERALISHEQDSPLPKTADPRVQIAGNYSPVPE 116
Query: 80 CSVRHDLEIIGNLPACLRGIYLRNGANPMHAPTGGHHLFDGDGMIHAVTFHSGNKASYSC 139
SVR +L + G +P C+ G+Y+RNGANPM PT GHHLFDGDGM+HAV +G+ ASY+C
Sbjct: 117 SSVRRNLTVEGTIPDCIDGVYIRNGANPMFEPTAGHHLFDGDGMVHAVKITNGS-ASYAC 176
Query: 140 RFTRTNRLQQEAALGRLVFPKPIGELHGHQGLARLAIFLARAGIGLIDGSKGTGVANAGL 199
RFT+T RL QE LGR VFPK IGELHGH G+ARL +F AR GLI+ G GVANAGL
Sbjct: 177 RFTKTERLVQEKRLGRPVFPKAIGELHGHSGIARLMLFYARGLCGLINNQNGVGVANAGL 236
Query: 200 VYFNGRLLALSEDDLPYHVQIKDDGDLETIGRFNFNGQINCPMIAHPKVDPISGDLHGLS 259
VYFN RLLA+SEDDLPY ++I GDL+T+GR++F+GQ+ MIAHPK+DP++ +LH LS
Sbjct: 237 VYFNNRLLAMSEDDLPYQLKITQTGDLQTVGRYDFDGQLKSAMIAHPKLDPVTKELHALS 296
Query: 260 YNMIKKPYLKYLRFDRFGKKSRDVDITLREPTMIHDFAITENHVVIPDHQVVFKLLEMVR 319
Y+++KKPYLKY RF G KS +++I L PTMIHDFAITEN VVIPD QVVFKL EM+
Sbjct: 297 YDVVKKPYLKYFRFSPDGVKSPELEIPLETPTMIHDFAITENFVVIPDQQVVFKLGEMIS 356
Query: 320 GGSPVVFDPKKTSRFGILPKSGVDEKGIVWIEVPNCFCFHLWNAWEETGGNDEKSIVVVG 379
G SPVVFD +K SR GI+PK + I+W+ P FCFHLWNAWE + + IVV+G
Sbjct: 357 GKSPVVFDGEKVSRLGIMPKDATEASQIIWVNSPETFCFHLWNAWESP---ETEEIVVIG 416
Query: 380 SCMNPPDSIFNDRDQPLRIELTEIRMDVKSRKVTRR--VFGSGMNLEAGQMNRGLVGRKT 439
SCM+P DSIFN+RD+ LR L+EIR+++++RK TRR + +NLE G +NR +GRKT
Sbjct: 417 SCMSPADSIFNERDESLRSVLSEIRINLRTRKTTRRSLLVNEDVNLEIGMVNRNRLGRKT 476
Query: 440 RFVYMAIADPWPKCSGIAKVDLETGKVKKFLYGEGRYGGEPFYVPENGNWGGDNEKEDGG 499
RF ++AIA PWPK SG AKVDL TG++KK++YG +YGGEPF++P GN G E ED G
Sbjct: 477 RFAFLAIAYPWPKVSGFAKVDLCTGEMKKYIYGGEKYGGEPFFLP--GNSGNGEENEDDG 536
Query: 500 YIVGFVRDEKRERSEVVVVKAAEMEEVAAVRLPVRVPYGFHGTFVSEEELNGQ 547
YI V DE+ + SE+ ++ A ++ A ++LP RVPYGFHGTFV EL Q
Sbjct: 537 YIFCHVHDEETKTSELQIINAVNLKLEATIKLPSRVPYGFHGTFVDSNELVDQ 582
BLAST of Cucsa.194320 vs. TAIR10
Match:
AT1G30100.1 (AT1G30100.1 nine-cis-epoxycarotenoid dioxygenase 5)
HSP 1 Score: 609.8 bits (1571), Expect = 1.7e-174
Identity = 302/523 (57.74%), Postives = 383/523 (73.23%), Query Frame = 1
Query: 32 RLNPLQKFAASLLDAVESSLFVDK----LPKTIDPAVQISGNFSPVPECSVRHDLEIIGN 91
R NPLQ+ A++ LD E++L + LPKT+DP QISGN++PVPE SV+ L + G
Sbjct: 75 RWNPLQRAASAALDFAETALLRRERSKPLPKTVDPRHQISGNYAPVPEQSVKSSLSVDGK 134
Query: 92 LPACLRGIYLRNGANPMHAPTGGHHLFDGDGMIHAVTFHSGNKASYSCRFTRTNRLQQEA 151
+P C+ G+YLRNGANP+ P GHHLFDGDGM+HAV +G+ ASYSCRFT T RL QE
Sbjct: 135 IPDCIDGVYLRNGANPLFEPVSGHHLFDGDGMVHAVKITNGD-ASYSCRFTETERLVQEK 194
Query: 152 ALGRLVFPKPIGELHGHQGLARLAIFLARAGIGLIDGSKGTGVANAGLVYFNGRLLALSE 211
LG +FPK IGELHGH G+ARL +F AR GL++ GTGVANAGLVYF+ RLLA+SE
Sbjct: 195 QLGSPIFPKAIGELHGHSGIARLMLFYARGLFGLLNHKNGTGVANAGLVYFHDRLLAMSE 254
Query: 212 DDLPYHVQIKDDGDLETIGRFNFNGQINCPMIAHPKVDPISGDLHGLSYNMIKKPYLKYL 271
DDLPY V++ D+GDLETIGRF+F+GQ++ MIAHPK+DP++ +L LSY+++KKPYLKY
Sbjct: 255 DDLPYQVRVTDNGDLETIGRFDFDGQLSSAMIAHPKIDPVTKELFALSYDVVKKPYLKYF 314
Query: 272 RFDRFGKKSRDVDITLREPTMIHDFAITENHVVIPDHQVVFKLLEMVRGGSPVVFDPKKT 331
+F G+KS DV+I L PTM+HDFAITEN VVIPD QVVFKL +M G SPV +D +K
Sbjct: 315 KFSPEGEKSPDVEIPLASPTMMHDFAITENFVVIPDQQVVFKLSDMFLGKSPVKYDGEKI 374
Query: 332 SRFGILPKSGVDEKGIVWIEVPNCFCFHLWNAWEETGGNDEKSIVVVGSCMNPPDSIFND 391
SRFGILP++ D +VW+E P FCFHLWNAWE + +VV+GSCM P DSIFN+
Sbjct: 375 SRFGILPRNAKDASEMVWVESPETFCFHLWNAWESP---ETDEVVVIGSCMTPADSIFNE 434
Query: 392 RDQPLRIELTEIRMDVKSRKVTRRVFGSG---MNLEAGQMNRGLVGRKTRFVYMAIADPW 451
D+ L L+EIR+++K+ K TRR G MNLEAG +NR L+GRKTR+ Y+AIA+PW
Sbjct: 435 CDEQLNSVLSEIRLNLKTGKSTRRTIIPGSVQMNLEAGMVNRNLLGRKTRYAYLAIAEPW 494
Query: 452 PKCSGIAKVDLETGKVKKFLYGEGRYGGEPFYVPENGNWGGDNEKEDGGYIVGFVRDEKR 511
PK SG AKVDL TG+VK YG +YGGEPF++P G +++ ED GYI+ FV DE+
Sbjct: 495 PKVSGFAKVDLSTGEVKNHFYGGKKYGGEPFFLPR----GLESDGEDDGYIMSFVHDEES 554
Query: 512 ERSEVVVVKAAEMEEVAAVRLPVRVPYGFHGTFVSEEELNGQA 548
SE+ +V A +E A V+LP RVPYGFHGTFV+ ++ QA
Sbjct: 555 WESELHIVNAVTLELEATVKLPSRVPYGFHGTFVNSADMLNQA 589
BLAST of Cucsa.194320 vs. NCBI nr
Match:
gi|449465258|ref|XP_004150345.1| (PREDICTED: 9-cis-epoxycarotenoid dioxygenase NCED6, chloroplastic [Cucumis sativus])
HSP 1 Score: 1114.8 bits (2882), Expect = 0.0e+00
Identity = 549/568 (96.65%), Postives = 549/568 (96.65%), Query Frame = 1
Query: 1 MQISLQSPPPPLTLCRSSSTIETPPIRKLK-------------------RRLNPLQKFAA 60
MQISLQSPPPPLTLCRSSSTIETPPIRKLK RRLNPLQKFAA
Sbjct: 1 MQISLQSPPPPLTLCRSSSTIETPPIRKLKVITPPRPPLSLPKKVSPPQRRLNPLQKFAA 60
Query: 61 SLLDAVESSLFVDKLPKTIDPAVQISGNFSPVPECSVRHDLEIIGNLPACLRGIYLRNGA 120
SLLDAVESSLFVDKLPKTIDPAVQISGNFSPVPECSVRHDLEIIGNLPACLRGIYLRNGA
Sbjct: 61 SLLDAVESSLFVDKLPKTIDPAVQISGNFSPVPECSVRHDLEIIGNLPACLRGIYLRNGA 120
Query: 121 NPMHAPTGGHHLFDGDGMIHAVTFHSGNKASYSCRFTRTNRLQQEAALGRLVFPKPIGEL 180
NPMHAPTGGHHLFDGDGMIHAVTFHSGNKASYSCRFTRTNRLQQEAALGRLVFPKPIGEL
Sbjct: 121 NPMHAPTGGHHLFDGDGMIHAVTFHSGNKASYSCRFTRTNRLQQEAALGRLVFPKPIGEL 180
Query: 181 HGHQGLARLAIFLARAGIGLIDGSKGTGVANAGLVYFNGRLLALSEDDLPYHVQIKDDGD 240
HGHQGLARLAIFLARAGIGLIDGSKGTGVANAGLVYFNGRLLALSEDDLPYHVQIKDDGD
Sbjct: 181 HGHQGLARLAIFLARAGIGLIDGSKGTGVANAGLVYFNGRLLALSEDDLPYHVQIKDDGD 240
Query: 241 LETIGRFNFNGQINCPMIAHPKVDPISGDLHGLSYNMIKKPYLKYLRFDRFGKKSRDVDI 300
LETIGRFNFNGQINCPMIAHPKVDPISGDLHGLSYNMIKKPYLKYLRFDRFGKKSRDVDI
Sbjct: 241 LETIGRFNFNGQINCPMIAHPKVDPISGDLHGLSYNMIKKPYLKYLRFDRFGKKSRDVDI 300
Query: 301 TLREPTMIHDFAITENHVVIPDHQVVFKLLEMVRGGSPVVFDPKKTSRFGILPKSGVDEK 360
TLREPTMIHDFAITENHVVIPDHQVVFKLLEMVRGGSPVVFDPKKTSRFGILPKSGVDEK
Sbjct: 301 TLREPTMIHDFAITENHVVIPDHQVVFKLLEMVRGGSPVVFDPKKTSRFGILPKSGVDEK 360
Query: 361 GIVWIEVPNCFCFHLWNAWEETGGNDEKSIVVVGSCMNPPDSIFNDRDQPLRIELTEIRM 420
GIVWIEVPNCFCFHLWNAWEETGGNDEKSIVVVGSCMNPPDSIFNDRDQPLRIELTEIRM
Sbjct: 361 GIVWIEVPNCFCFHLWNAWEETGGNDEKSIVVVGSCMNPPDSIFNDRDQPLRIELTEIRM 420
Query: 421 DVKSRKVTRRVFGSGMNLEAGQMNRGLVGRKTRFVYMAIADPWPKCSGIAKVDLETGKVK 480
DVKSRKVTRRVFGSGMNLEAGQMNRGLVGRKTRFVYMAIADPWPKCSGIAKVDLETGKVK
Sbjct: 421 DVKSRKVTRRVFGSGMNLEAGQMNRGLVGRKTRFVYMAIADPWPKCSGIAKVDLETGKVK 480
Query: 481 KFLYGEGRYGGEPFYVPENGNWGGDNEKEDGGYIVGFVRDEKRERSEVVVVKAAEMEEVA 540
KFLYGEGRYGGEPFYVPENGNWGGDNEKEDGGYIVGFVRDEKRERSEVVVVKAAEMEEVA
Sbjct: 481 KFLYGEGRYGGEPFYVPENGNWGGDNEKEDGGYIVGFVRDEKRERSEVVVVKAAEMEEVA 540
Query: 541 AVRLPVRVPYGFHGTFVSEEELNGQARN 550
AVRLPVRVPYGFHGTFVSEEELNGQARN
Sbjct: 541 AVRLPVRVPYGFHGTFVSEEELNGQARN 568
BLAST of Cucsa.194320 vs. NCBI nr
Match:
gi|659121571|ref|XP_008460725.1| (PREDICTED: 9-cis-epoxycarotenoid dioxygenase NCED6, chloroplastic [Cucumis melo])
HSP 1 Score: 1069.3 bits (2764), Expect = 2.2e-309
Identity = 526/568 (92.61%), Postives = 535/568 (94.19%), Query Frame = 1
Query: 1 MQISLQSPPPPLTLCRSSSTIETPPIRKLK-------------------RRLNPLQKFAA 60
MQISL SP PPLT CRSSSTIETPPIRKLK R+LNPLQ FAA
Sbjct: 1 MQISLLSPTPPLTPCRSSSTIETPPIRKLKVITPPHPPLSLPKKVSPPLRQLNPLQNFAA 60
Query: 61 SLLDAVESSLFVDKLPKTIDPAVQISGNFSPVPECSVRHDLEIIGNLPACLRGIYLRNGA 120
SLLDAVESSLFVDKLPKT+DPAVQISGNFSPVPECSVRHDLEIIGNLPACLRG+YLRNGA
Sbjct: 61 SLLDAVESSLFVDKLPKTVDPAVQISGNFSPVPECSVRHDLEIIGNLPACLRGVYLRNGA 120
Query: 121 NPMHAPTGGHHLFDGDGMIHAVTFHSGNKASYSCRFTRTNRLQQEAALGRLVFPKPIGEL 180
NPMHAPTGGHHLFDGDGMIHAVTFHS NKASYSCRFTRTNRLQ EAALGR VFPKPIGEL
Sbjct: 121 NPMHAPTGGHHLFDGDGMIHAVTFHSNNKASYSCRFTRTNRLQHEAALGRPVFPKPIGEL 180
Query: 181 HGHQGLARLAIFLARAGIGLIDGSKGTGVANAGLVYFNGRLLALSEDDLPYHVQIKDDGD 240
HGHQGLARLAIFLARAGIG+IDGSKGTGVANAGLVYFNGRLLALSEDDLPYHVQIKDDGD
Sbjct: 181 HGHQGLARLAIFLARAGIGIIDGSKGTGVANAGLVYFNGRLLALSEDDLPYHVQIKDDGD 240
Query: 241 LETIGRFNFNGQINCPMIAHPKVDPISGDLHGLSYNMIKKPYLKYLRFDRFGKKSRDVDI 300
LETIGRF+FNGQINCPMIAHPKVDPISGDLHGLSYNMIKKPYLKYLRFD FGKKSRDVDI
Sbjct: 241 LETIGRFDFNGQINCPMIAHPKVDPISGDLHGLSYNMIKKPYLKYLRFDMFGKKSRDVDI 300
Query: 301 TLREPTMIHDFAITENHVVIPDHQVVFKLLEMVRGGSPVVFDPKKTSRFGILPKSGVDEK 360
TLREPTMIHDFAITENHVVIPDHQVVFKL EMVRGGSPVVFDPKKTSRFGILPKSGVDEK
Sbjct: 301 TLREPTMIHDFAITENHVVIPDHQVVFKLFEMVRGGSPVVFDPKKTSRFGILPKSGVDEK 360
Query: 361 GIVWIEVPNCFCFHLWNAWEETGGNDEKSIVVVGSCMNPPDSIFNDRDQPLRIELTEIRM 420
GIVWIEVPNCFCFHLWNAWEETGGNDEKSIVV+GSCMNPPDSIFNDRDQPLRIELTEIRM
Sbjct: 361 GIVWIEVPNCFCFHLWNAWEETGGNDEKSIVVIGSCMNPPDSIFNDRDQPLRIELTEIRM 420
Query: 421 DVKSRKVTRRVFGSGMNLEAGQMNRGLVGRKTRFVYMAIADPWPKCSGIAKVDLETGKVK 480
DVK+RKVTRRVFGSGMNLEAGQMNR VGRKTRFVYMAIA+PWPKCSGIAKVDLETGKVK
Sbjct: 421 DVKNRKVTRRVFGSGMNLEAGQMNRRFVGRKTRFVYMAIAEPWPKCSGIAKVDLETGKVK 480
Query: 481 KFLYGEGRYGGEPFYVPENGNWGGDNEKEDGGYIVGFVRDEKRERSEVVVVKAAEMEEVA 540
KFLYGEGRYGGEPFYV ENGN GGDNE EDGG+IVGFVRDEKRERSEVVVVKAAEMEEVA
Sbjct: 481 KFLYGEGRYGGEPFYVSENGNCGGDNENEDGGFIVGFVRDEKRERSEVVVVKAAEMEEVA 540
Query: 541 AVRLPVRVPYGFHGTFVSEEELNGQARN 550
AVRLPVRVPYGFHGTFVSEEELNGQARN
Sbjct: 541 AVRLPVRVPYGFHGTFVSEEELNGQARN 568
BLAST of Cucsa.194320 vs. NCBI nr
Match:
gi|289468083|gb|ADC95722.1| (9-cis epoxycarotenoid dioxygenase [Citrullus lanatus])
HSP 1 Score: 818.5 bits (2113), Expect = 6.8e-234
Identity = 392/422 (92.89%), Postives = 413/422 (97.87%), Query Frame = 1
Query: 113 LFDGDGMIHAVTFHSGNKASYSCRFTRTNRLQQEAALGRLVFPKPIGELHGHQGLARLAI 172
LFDGDGMIHAVTFH GN+ASYSCRFTRT+RLQQEA+LGR VFPKPIGELHGHQGLARLAI
Sbjct: 1 LFDGDGMIHAVTFHCGNRASYSCRFTRTSRLQQEASLGRPVFPKPIGELHGHQGLARLAI 60
Query: 173 FLARAGIGLIDGSKGTGVANAGLVYFNGRLLALSEDDLPYHVQIKDDGDLETIGRFNFNG 232
FLARAGIGLIDGSKGTGVANAGLVYFNGRLLALSEDDLPYHV+IKDDGDLETIGRFNFNG
Sbjct: 61 FLARAGIGLIDGSKGTGVANAGLVYFNGRLLALSEDDLPYHVKIKDDGDLETIGRFNFNG 120
Query: 233 QINCPMIAHPKVDPISGDLHGLSYNMIKKPYLKYLRFDRFGKKSRDVDITLREPTMIHDF 292
QINCP+IAHPKVDPISGDLHGLSYNMIKKPYLKYLRFDRFGKKSRDVDI+LREPTMIHDF
Sbjct: 121 QINCPIIAHPKVDPISGDLHGLSYNMIKKPYLKYLRFDRFGKKSRDVDISLREPTMIHDF 180
Query: 293 AITENHVVIPDHQVVFKLLEMVRGGSPVVFDPKKTSRFGILPKSGVDEKGIVWIEVPNCF 352
AITENHVVIPDHQVVFKL EM+RGGSPVVFDPKKTSRFGILPKSGVDEKGIVWIEVP+CF
Sbjct: 181 AITENHVVIPDHQVVFKLSEMIRGGSPVVFDPKKTSRFGILPKSGVDEKGIVWIEVPDCF 240
Query: 353 CFHLWNAWEETGGNDEKSIVVVGSCMNPPDSIFNDRDQPLRIELTEIRMDVKSRKVTRRV 412
CFHLWNAWEETG NDE+++VVVGSCMNPPDSIFNDRDQPLRIELTEIRMDV++RKVTRRV
Sbjct: 241 CFHLWNAWEETGDNDEETVVVVGSCMNPPDSIFNDRDQPLRIELTEIRMDVRNRKVTRRV 300
Query: 413 FGSGMNLEAGQMNRGLVGRKTRFVYMAIADPWPKCSGIAKVDLETGKVKKFLYGEGRYGG 472
FGSG+NLEAGQ+NR LVG++TRFVYMAIA+PWPKCSGIAKVDLETGKVKKFLYG+GRYGG
Sbjct: 301 FGSGLNLEAGQVNRRLVGQRTRFVYMAIAEPWPKCSGIAKVDLETGKVKKFLYGDGRYGG 360
Query: 473 EPFYVPENGNWGGDNEKEDGGYIVGFVRDEKRERSEVVVVKAAEMEEVAAVRLPVRVPYG 532
EPFYV ENGN GGD+E EDGG+IVGFVRDEKRERSEVVVVKAAEMEEVAAVRLP RVPYG
Sbjct: 361 EPFYVSENGNHGGDHENEDGGFIVGFVRDEKRERSEVVVVKAAEMEEVAAVRLPARVPYG 420
Query: 533 FH 535
FH
Sbjct: 421 FH 422
BLAST of Cucsa.194320 vs. NCBI nr
Match:
gi|255554198|ref|XP_002518139.1| (PREDICTED: 9-cis-epoxycarotenoid dioxygenase NCED6, chloroplastic [Ricinus communis])
HSP 1 Score: 783.5 bits (2022), Expect = 2.4e-223
Identity = 381/570 (66.84%), Postives = 459/570 (80.53%), Query Frame = 1
Query: 2 QISLQSPPPPLTLCRSSS-----TIETPPIRKLKRRLNPLQKFAASLLDAVESSLFVD-- 61
++ L PPPPL+ S T TP LNPLQK AA LD +ESSL V
Sbjct: 47 KLPLSLPPPPLSEPAPPSLEPGPTFTTPRETDHPIALNPLQKLAALALDKIESSLLVPFE 106
Query: 62 ---KLPKTIDPAVQISGNFSPVPECSVRHDLEIIGNLPACLRGIYLRNGANPMHAPTGGH 121
LP+TIDP VQISGNF+PV EC V H LE++G++P LRG+YLRNGANP++APTGGH
Sbjct: 107 RNHLLPRTIDPTVQISGNFAPVLECPVHHGLEVVGHIPNSLRGVYLRNGANPVYAPTGGH 166
Query: 122 HLFDGDGMIHAVTFHSGNKASYSCRFTRTNRLQQEAALGRLVFPKPIGELHGHQGLARLA 181
HLFDGDGM+HAVT N+ASYSCRFTRT+RL+QEAALGR VFPKPIGELHGH G ARL
Sbjct: 167 HLFDGDGMVHAVTLGYENRASYSCRFTRTSRLEQEAALGRSVFPKPIGELHGHLGFARLL 226
Query: 182 IFLARAGIGLIDGSKGTGVANAGLVYFNGRLLALSEDDLPYHVQIKDDGDLETIGRFNFN 241
IF+ RAGIGL+DGS+GTGVANAGLVYFNGRLLA+SEDDLPYHV+IK DGDLETI RF+F+
Sbjct: 227 IFMVRAGIGLVDGSRGTGVANAGLVYFNGRLLAMSEDDLPYHVKIKGDGDLETIKRFSFD 286
Query: 242 GQINCPMIAHPKVDPISGDLHGLSYNMIKKPYLKYLRFDRFGKKSRDVDITLREPTMIHD 301
Q++CPMIAHPKVDP++G+LH LSYN+++KPYLKY +FD++GKKSRD+DITL +PTMIHD
Sbjct: 287 DQLDCPMIAHPKVDPVTGELHALSYNVVQKPYLKYFKFDKYGKKSRDLDITLHQPTMIHD 346
Query: 302 FAITENHVVIPDHQVVFKLLEMVRGGSPVVFDPKKTSRFGILPKSGVDEKGIVWIEVPNC 361
FA+T N VVIPDHQVVFKL EM+RGGSPV++D KTSRFGIL K+ V+E GI WIEVPNC
Sbjct: 347 FAVTRNFVVIPDHQVVFKLSEMIRGGSPVIYDKNKTSRFGILSKNEVNESGIQWIEVPNC 406
Query: 362 FCFHLWNAWEETGGNDEKSIVVVGSCMNPPDSIFNDRDQPLRIELTEIRMDVKSRKVTRR 421
FCFHLWN+WEE N +K IVV+GSCMNPPDSIFN+ + ++ EL+EIR+++++R+ TR+
Sbjct: 407 FCFHLWNSWEEISTNGDKIIVVIGSCMNPPDSIFNESETAIQSELSEIRLNMRTRESTRK 466
Query: 422 VFGSGMNLEAGQMNRGLVGRKTRFVYMAIADPWPKCSGIAKVDLETGKVKKFLYGEGRYG 481
GMNLEAGQ+NR VG+KTR+VY+AIA+PWPKCSGIAKVDLET +V KF+YG+GRYG
Sbjct: 467 AIVRGMNLEAGQVNRKFVGQKTRYVYLAIAEPWPKCSGIAKVDLETKEVTKFMYGKGRYG 526
Query: 482 GEPFYVPEN------------GNWGGD--NEKEDGGYIVGFVRDEKRERSEVVVVKAAEM 541
GEPF+V +N G G+ N++E GYI+GFVRDE +ERSE+V+V A M
Sbjct: 527 GEPFFVAKNREGEEEEDDDDDGKKIGNVINDEEGEGYIMGFVRDEIKERSELVIVNATGM 586
Query: 542 EEVAAVRLPVRVPYGFHGTFVSEEELNGQA 548
E+VA+V LP RVPYGFHGTFVSE+EL GQA
Sbjct: 587 EQVASVSLPTRVPYGFHGTFVSEDELKGQA 616
BLAST of Cucsa.194320 vs. NCBI nr
Match:
gi|590657059|ref|XP_007034452.1| (Nine-cis-epoxycarotenoid dioxygenase 6 [Theobroma cacao])
HSP 1 Score: 780.4 bits (2014), Expect = 2.1e-222
Identity = 380/558 (68.10%), Postives = 457/558 (81.90%), Query Frame = 1
Query: 8 PPPPLTLCRSSSTIETPPIRKLKR-----------RLNPLQKFAASLLDAVESSLF--VD 67
PPPP RS E PI K +R LNPLQK AA LD +E+S+ ++
Sbjct: 50 PPPP---SRSPLYPEVEPIVKPERVLVPHEPNYPVHLNPLQKLAALALDQIETSVIKPLE 109
Query: 68 K---LPKTIDPAVQISGNFSPVPECSVRHDLEIIGNLPACLRGIYLRNGANPMHAPTGGH 127
K LPKT+DPAVQISGNF+PV ECSV+H LE++G +PACLRG+Y+RNGANPM AP+GGH
Sbjct: 110 KNHDLPKTVDPAVQISGNFAPVQECSVQHWLEVVGQIPACLRGVYVRNGANPMFAPSGGH 169
Query: 128 HLFDGDGMIHAVTFHSGNKASYSCRFTRTNRLQQEAALGRLVFPKPIGELHGHQGLARLA 187
HLFDGDGMIHAV+ GN+ASYSCR+TRT+RL QEA LGR +FPKPIGELHGH GLARLA
Sbjct: 170 HLFDGDGMIHAVSLGRGNEASYSCRYTRTSRLMQEARLGRSIFPKPIGELHGHLGLARLA 229
Query: 188 IFLARAGIGLIDGSKGTGVANAGLVYFNGRLLALSEDDLPYHVQIKDDGDLETIGRFNFN 247
+F+AR G+GL+DGS GTGVANAGLVYFNGRLLA+SEDDLPYHV+I D DLET+GRFNF+
Sbjct: 230 LFMARDGVGLVDGSHGTGVANAGLVYFNGRLLAMSEDDLPYHVKINADADLETMGRFNFD 289
Query: 248 GQINCPMIAHPKVDPISGDLHGLSYNMIKKPYLKYLRFDRFGKKSRDVDITLREPTMIHD 307
QI+CP+IAHPKVDP++G+LH L+YN++KKPYLKY +FD+FGKKSRD+ +++ + TMIHD
Sbjct: 290 DQIDCPLIAHPKVDPVTGELHTLNYNVLKKPYLKYFKFDKFGKKSRDLHVSIEQATMIHD 349
Query: 308 FAITENHVVIPDHQVVFKLLEMVRGGSPVVFDPKKTSRFGILPKSGVDEKGIVWIEVPNC 367
FAITEN VVIPDHQVVFKL EM+RGGSPV++D KKTSRFGIL ++ VD I WIEVPNC
Sbjct: 350 FAITENFVVIPDHQVVFKLSEMIRGGSPVLYDKKKTSRFGILQQNDVDGSNIQWIEVPNC 409
Query: 368 FCFHLWNAWEETGGNDEKSIVVVGSCMNPPDSIFNDRDQPLRIELTEIRMDVKSRKVTRR 427
FCFHLWNAWEE N +K IVV+GSCM+PPDS+FN+ P R EL+EIR+++++ + T+R
Sbjct: 410 FCFHLWNAWEEHSDNGDKIIVVIGSCMSPPDSVFNESKDPFRSELSEIRLNLRTGESTQR 469
Query: 428 VFGSGMNLEAGQMNRGLVGRKTRFVYMAIADPWPKCSGIAKVDLETGKVKKFLYGEGRYG 487
V SG NLEAGQ+NR GRKTRFVY+AIA+PWPKCSGI KVDLETG+V KF+YG R+G
Sbjct: 470 VVISGTNLEAGQVNRQYQGRKTRFVYLAIAEPWPKCSGITKVDLETGEVTKFMYGACRFG 529
Query: 488 GEPFYVPE-NGNWGGD--NEKEDGGYIVGFVRDEKRERSEVVVVKAAEMEEVAAVRLPVR 547
GEPF+VPE GG+ NE ED GYI+GFVRDEK+E SE+V+VKA ME+VA++RLP R
Sbjct: 530 GEPFFVPEKKQKHGGNIANEAEDEGYIMGFVRDEKKEISELVIVKALSMEQVASIRLPTR 589
The following BLAST results are available for this feature:
Match Name | E-value | Identity | Description | |
NCED6_ARATH | 1.9e-199 | 61.85 | 9-cis-epoxycarotenoid dioxygenase NCED6, chloroplastic OS=Arabidopsis thaliana G... | [more] |
NCED9_ARATH | 1.7e-184 | 57.87 | 9-cis-epoxycarotenoid dioxygenase NCED9, chloroplastic OS=Arabidopsis thaliana G... | [more] |
NCED1_MAIZE | 2.7e-182 | 58.82 | 9-cis-epoxycarotenoid dioxygenase 1, chloroplastic OS=Zea mays GN=VP14 PE=1 SV=2 | [more] |
NCED3_ARATH | 8.8e-181 | 56.94 | 9-cis-epoxycarotenoid dioxygenase NCED3, chloroplastic OS=Arabidopsis thaliana G... | [more] |
NCED_ONCHC | 1.1e-180 | 58.40 | 9-cis-epoxycarotenoid dioxygenase, chloroplastic OS=Oncidium hybrid cultivar GN=... | [more] |
Match Name | E-value | Identity | Description | |
A0A0A0LIE2_CUCSA | 0.0e+00 | 96.65 | Uncharacterized protein OS=Cucumis sativus GN=Csa_2G160620 PE=4 SV=1 | [more] |
D5G3I2_CITLA | 4.8e-234 | 92.89 | 9-cis epoxycarotenoid dioxygenase (Fragment) OS=Citrullus lanatus GN=NCED1 PE=2 ... | [more] |
B9RWM0_RICCO | 1.7e-223 | 66.84 | 9-cis-epoxycarotenoid dioxygenase, putative OS=Ricinus communis GN=RCOM_1021470 ... | [more] |
A0A061EL25_THECC | 1.4e-222 | 68.10 | Nine-cis-epoxycarotenoid dioxygenase 6 OS=Theobroma cacao GN=TCM_020388 PE=4 SV=... | [more] |
V4S0R4_9ROSI | 7.9e-221 | 66.85 | Uncharacterized protein OS=Citrus clementina GN=CICLE_v10006710mg PE=4 SV=1 | [more] |