Cucsa.193420 (gene) Cucumber (Gy14) v1

BLAST of Cucsa.193420 vs. Swiss-Prot
Match: SSG1_ANTMA (Granule-bound starch synthase 1, chloroplastic/amyloplastic OS=Antirrhinum majus GN=WAXY PE=2 SV=1)

HSP 1 Score: 907.5 bits (2344), Expect = 7.8e-263
Identity = 446/607 (73.48%), Postives = 513/607 (84.51%), Query Frame = 1

Query: 13  VSRSSLISH---GATAS-ETKMSLS-VGGKNQVETHKGLKSLNNLDVLRKRTPASAICRT 72
           V+ S L+SH   GAT+S +TK +L+ VG +NQ  TH GL+S+N +D L+ R  A    R+
Sbjct: 4   VTASQLVSHVHGGATSSPDTKTNLAQVGLRNQQFTHNGLRSINMVDKLQMRNNAKQ-SRS 63

Query: 73  VSRKTDGLGGTAGKIVCEQGMNIVFVAAEVTPWSKTGGLGDVLGGLPPAMAARGHRVMTI 132
           + +KTD  G   GKI+C  GMN+VFV AEV PWSKTGGLGDV+GGLPPAMA  GHRVMT+
Sbjct: 64  LVKKTDN-GSPLGKIICGTGMNLVFVLAEVGPWSKTGGLGDVVGGLPPAMAGNGHRVMTV 123

Query: 133 APRYDQYKDAWDTDVTVEIQVGDAILTVRFFHCYKRGVDRVFVDHPIFLEKVWGKTKSKL 192
           +PRYDQYKDAWDT V VEI+VGD+I TVRFFHCYKRGVDRVFVDHPIFLEKVWGKTKSK+
Sbjct: 124 SPRYDQYKDAWDTSVVVEIKVGDSIETVRFFHCYKRGVDRVFVDHPIFLEKVWGKTKSKI 183

Query: 193 YGPNAGVDYDDNQLRFSLLCQAALLAPLVLNLNSSKYFSGPYGEEVVFVANDWHTALLPC 252
           YGPNAG DY DNQLRFSLLCQAAL AP VLNL SSKYFSGPYGE+VVFVANDWHTALLPC
Sbjct: 184 YGPNAGTDYQDNQLRFSLLCQAALEAPRVLNLTSSKYFSGPYGEDVVFVANDWHTALLPC 243

Query: 253 YLKTMYQARGIYKSAKVAFCIHNIAYQGRFAFSDFSLLNLPDQFKSSFDFTDGYVKPVKG 312
           YLK+MYQ++G+Y  AKVAFCIHNIAYQGRF  SDF LLNLPDQFKSSFDF DGY KPVKG
Sbjct: 244 YLKSMYQSKGMYLHAKVAFCIHNIAYQGRFGSSDFCLLNLPDQFKSSFDFFDGYEKPVKG 303

Query: 313 RKINWMKAGILESDRVLTVSPYYAQELVSGIERGVELDSILRLTTVTGILNGMDVQEWNP 372
           RKINWMKAGILESDRV+TVSPYYA ELVSG E+GVELD+++  T++TGI+NGMD QEWNP
Sbjct: 304 RKINWMKAGILESDRVVTVSPYYAMELVSGAEKGVELDNVIAKTSITGIVNGMDTQEWNP 363

Query: 373 STDKYIPIKFNAETVAEAKALLKEALQAEVGLPVDRNIPLIGFIGRLEEQKGSDILSAAI 432
           +TDK+I   ++  TV +AK LLKEALQA VGLPVD+NIP+IGFIGRLEEQKGSDIL AAI
Sbjct: 364 ATDKHIDTNYDITTVMDAKPLLKEALQAAVGLPVDKNIPVIGFIGRLEEQKGSDILVAAI 423

Query: 433 PNFIHEDVQLVVLGTGKKYLEKQLEELEIKYPDKARGVAKFNVPLAHLINAAADFLMIPS 492
             F+  DVQ+++LGTGKK  E+Q++ELE+ YPDKARGVAKFNVPLAH+I A ADF+++PS
Sbjct: 424 SKFVGLDVQIIILGTGKKKFEQQIQELEVLYPDKARGVAKFNVPLAHMITAGADFMLVPS 483

Query: 493 RFEPCGLIQLQAMPYGTIPLVSTTGGLADTVIDGYTGLQMGAFSVECETVSPADAAAIAK 552
           RFEPCGLIQL AM YGTIP+ ++TGGL DTV +G+TG  MGAF+VEC TV PAD   IA 
Sbjct: 484 RFEPCGLIQLHAMRYGTIPICASTGGLVDTVTEGFTGFHMGAFNVECATVDPADVQKIAT 543

Query: 553 TVLRAVALFGTAAHKEMIQNCMAQDFSWKGPAKQWEKFLLGLGVENSQAGIEGEEIAPLA 612
           TV RA+A +G+ A+KEMIQNCMAQD SWKGPAK WEK LL LGV  S+ G++GEEIAPLA
Sbjct: 544 TVERALAAYGSVAYKEMIQNCMAQDLSWKGPAKNWEKMLLSLGVSGSEPGVDGEEIAPLA 603

Query: 613 KENVATP 615
           KENVATP
Sbjct: 604 KENVATP 608

BLAST of Cucsa.193420 vs. Swiss-Prot
Match: SSG1_MANES (Granule-bound starch synthase 1, chloroplastic/amyloplastic OS=Manihot esculenta GN=WAXY PE=2 SV=1)

HSP 1 Score: 890.2 bits (2299), Expect = 1.3e-257
Identity = 437/611 (71.52%), Postives = 496/611 (81.18%), Query Frame = 1

Query: 4   MGAVSVSNFVSRSSLISHGATASETKMSLSVGGKNQVETHKGLKSLNNLDVLRKRTPASA 63
           M  V  ++FVSRSS +S  A  ++       G   Q  T  GL+SLN +D L+ +T + A
Sbjct: 1   MATVIAAHFVSRSSHLSIHALETKANNLSHTGPWTQTITPNGLRSLNTMDKLQMKTQSKA 60

Query: 64  ICRTVSRKTDGLGGTAGKIVCEQGMNIVFVAAEVTPWSKTGGLGDVLGGLPPAMAARGHR 123
           + +       G G  A KI+C  GMN++FV AEV PWSKTGGLGDVLGGLPPAMAARGHR
Sbjct: 61  VKKV---SATGNGRPAAKIICGHGMNLIFVGAEVGPWSKTGGLGDVLGGLPPAMAARGHR 120

Query: 124 VMTIAPRYDQYKDAWDTDVTVEIQVGDAILTVRFFHCYKRGVDRVFVDHPIFLEKVWGKT 183
           VMT++PRYDQYKDAWDT V+VEI++GD I TVRFFH YKRGVDRVFVDHP+FLEKVWGKT
Sbjct: 121 VMTVSPRYDQYKDAWDTSVSVEIKIGDRIETVRFFHSYKRGVDRVFVDHPMFLEKVWGKT 180

Query: 184 KSKLYGPNAGVDYDDNQLRFSLLCQAALLAPLVLNLNSSKYFSGPYGEEVVFVANDWHTA 243
            SK+YGP AG+DY DNQLRFSLLC AAL AP VLNLNSSK FSGPYGEEV F+ANDWHTA
Sbjct: 181 GSKIYGPRAGLDYQDNQLRFSLLCLAALEAPRVLNLNSSKNFSGPYGEEVAFIANDWHTA 240

Query: 244 LLPCYLKTMYQARGIYKSAKVAFCIHNIAYQGRFAFSDFSLLNLPDQFKSSFDFTDGYVK 303
           LLPCYLK +YQ  GIYK AKVAFCIHNIAYQGRFAFSDF  LNLPD+FKSSFDF DGY K
Sbjct: 241 LLPCYLKAIYQPMGIYKHAKVAFCIHNIAYQGRFAFSDFPRLNLPDKFKSSFDFIDGYEK 300

Query: 304 PVKGRKINWMKAGILESDRVLTVSPYYAQELVSGIERGVELDSILRLTTVTGILNGMDVQ 363
           PVKGRKINWMKAGILESDRVLTVSPYYAQE++SG+ERGVELD+ +R T + GI+NGMDVQ
Sbjct: 301 PVKGRKINWMKAGILESDRVLTVSPYYAQEVISGVERGVELDNFIRKTGIAGIINGMDVQ 360

Query: 364 EWNPSTDKYIPIKFNAETVAEAKALLKEALQAEVGLPVDRNIPLIGFIGRLEEQKGSDIL 423
           EWNP TDKYI I ++A TV +AK LLKEALQAEVGLPVDRN+PLIGFIGRLEEQKGSDI 
Sbjct: 361 EWNPVTDKYIDIHYDATTVMDAKPLLKEALQAEVGLPVDRNVPLIGFIGRLEEQKGSDIF 420

Query: 424 SAAIPNFIHEDVQLVVLGTGKKYLEKQLEELEIKYPDKARGVAKFNVPLAHLINAAADFL 483
            AAI   +  +VQ+V+LGTGKK  EKQ+E LE+ YPDKARGVAKFNVPLAH+I A ADF+
Sbjct: 421 VAAISQLVEHNVQIVILGTGKKKFEKQIEHLEVLYPDKARGVAKFNVPLAHMITAGADFM 480

Query: 484 MIPSRFEPCGLIQLQAMPYGTIPLVSTTGGLADTVIDGYTGLQMGAFSVECETVSPADAA 543
           ++PSRFEPCGLIQL AM YGT+P+V++TGGL DTV +GYTG QMGA  VEC+ +  AD A
Sbjct: 481 LVPSRFEPCGLIQLHAMRYGTVPIVASTGGLVDTVKEGYTGFQMGALHVECDKIDSADVA 540

Query: 544 AIAKTVLRAVALFGTAAHKEMIQNCMAQDFSWKGPAKQWEKFLLGLGVENSQAGIEGEEI 603
           AI KTV RA+  + TAA +EMI NCMAQD SWKGPA+ WEK LL L V  S+ G EGEEI
Sbjct: 541 AIVKTVARALGTYATAALREMILNCMAQDLSWKGPARMWEKMLLDLEVTGSEPGTEGEEI 600

Query: 604 APLAKENVATP 615
           APLAKENV TP
Sbjct: 601 APLAKENVPTP 608

BLAST of Cucsa.193420 vs. Swiss-Prot
Match: SSG1_SOLTU (Granule-bound starch synthase 1, chloroplastic/amyloplastic OS=Solanum tuberosum GN=WAXY PE=1 SV=1)

HSP 1 Score: 882.9 bits (2280), Expect = 2.1e-255
Identity = 428/613 (69.82%), Postives = 507/613 (82.71%), Query Frame = 1

Query: 4   MGAVSVSN-FVSRSSLISHGATASETKMSLS-VGGKNQVETHKGLKSLNNLDVLRKRTPA 63
           M +++ S+ FVSRS       T+ +TK +LS +G +N   TH GL+++N LD L+ RT  
Sbjct: 1   MASITASHHFVSRSQ------TSLDTKSTLSQIGLRNHTLTHNGLRAVNKLDGLQSRTNT 60

Query: 64  SAICRTVSRKTDGLGGTAGKIVCEQGMNIVFVAAEVTPWSKTGGLGDVLGGLPPAMAARG 123
               +  SR      G +  IVC +GMN++FV  EV PWSKTGGLGDVLGGLPPA+AARG
Sbjct: 61  KVTPKMASRTETKRPGCSATIVCGKGMNLIFVGTEVGPWSKTGGLGDVLGGLPPALAARG 120

Query: 124 HRVMTIAPRYDQYKDAWDTDVTVEIQVGDAILTVRFFHCYKRGVDRVFVDHPIFLEKVWG 183
           HRVMTI+PRYDQYKDAWDT V VE++VGD+I  VRFFHCYKRGVDRVFVDHP+FLEKVWG
Sbjct: 121 HRVMTISPRYDQYKDAWDTSVAVEVKVGDSIEIVRFFHCYKRGVDRVFVDHPMFLEKVWG 180

Query: 184 KTKSKLYGPNAGVDYDDNQLRFSLLCQAALLAPLVLNLNSSKYFSGPYGEEVVFVANDWH 243
           KT SK+YGP AG+DY DN+LRFSLLCQAAL AP VLNLNSS YFSGPYGE+V+F+ANDWH
Sbjct: 181 KTGSKIYGPKAGLDYLDNELRFSLLCQAALEAPKVLNLNSSNYFSGPYGEDVLFIANDWH 240

Query: 244 TALLPCYLKTMYQARGIYKSAKVAFCIHNIAYQGRFAFSDFSLLNLPDQFKSSFDFTDGY 303
           TAL+PCYLK+MYQ+RGIY +AKVAFCIHNIAYQGRF+FSDF LLNLPD+F+ SFDF DGY
Sbjct: 241 TALIPCYLKSMYQSRGIYLNAKVAFCIHNIAYQGRFSFSDFPLLNLPDEFRGSFDFIDGY 300

Query: 304 VKPVKGRKINWMKAGILESDRVLTVSPYYAQELVSGIERGVELDSILRLTTVTGILNGMD 363
            KPVKGRKINWMKAGILES RV+TVSPYYAQELVS +++GVELDS+LR T +TGI+NGMD
Sbjct: 301 EKPVKGRKINWMKAGILESHRVVTVSPYYAQELVSAVDKGVELDSVLRKTCITGIVNGMD 360

Query: 364 VQEWNPSTDKYIPIKFNAETVAEAKALLKEALQAEVGLPVDRNIPLIGFIGRLEEQKGSD 423
            QEWNP+TDKY  +K++  TV +AK LLKEALQA VGLPVD+ IPLIGFIGRLEEQKGSD
Sbjct: 361 TQEWNPATDKYTDVKYDITTVMDAKPLLKEALQAAVGLPVDKKIPLIGFIGRLEEQKGSD 420

Query: 424 ILSAAIPNFIHEDVQLVVLGTGKKYLEKQLEELEIKYPDKARGVAKFNVPLAHLINAAAD 483
           IL AAI  FI  DVQ+VVLGTGKK  E+++E+LE+ YP+KA+GVAKFNVPLAH+I A AD
Sbjct: 421 ILVAAIHKFIGLDVQIVVLGTGKKEFEQEIEQLEVLYPNKAKGVAKFNVPLAHMITAGAD 480

Query: 484 FLMIPSRFEPCGLIQLQAMPYGTIPLVSTTGGLADTVIDGYTGLQMGAFSVECETVSPAD 543
           F+++PSRFEPCGLIQL AM YGT+P+ ++TGGL DTV +GYTG  MGAF+VEC+ V PAD
Sbjct: 481 FMLVPSRFEPCGLIQLHAMRYGTVPICASTGGLVDTVKEGYTGFHMGAFNVECDVVDPAD 540

Query: 544 AAAIAKTVLRAVALFGTAAHKEMIQNCMAQDFSWKGPAKQWEKFLLGLGVENSQAGIEGE 603
              I  TV RA+A++GT A  EMI+NCM+++ SWK PAK+WE  LLGLG   S+ G+EGE
Sbjct: 541 VLKIVTTVARALAVYGTLAFAEMIKNCMSEELSWKEPAKKWETLLLGLGASGSEPGVEGE 600

Query: 604 EIAPLAKENVATP 615
           EIAPLAKENVATP
Sbjct: 601 EIAPLAKENVATP 607

BLAST of Cucsa.193420 vs. Swiss-Prot
Match: SSG1_ARATH (Granule-bound starch synthase 1, chloroplastic/amyloplastic OS=Arabidopsis thaliana GN=GBSS1 PE=1 SV=1)

HSP 1 Score: 878.6 bits (2269), Expect = 3.9e-254
Identity = 432/606 (71.29%), Postives = 506/606 (83.50%), Query Frame = 1

Query: 10  SNFVSRSSLIS-HGATASETKMSLSVGGKNQVETHKGLKSLNNLDVLRKRTPASAICRTV 69
           SNFVSR+SL + HGA++      +++  K Q  TH GL+S N +D L++R+ A  +    
Sbjct: 8   SNFVSRTSLFNNHGASSCSDVAQITL--KGQSLTHCGLRSFNMVDNLQRRSQAKPVSAKS 67

Query: 70  SRKTDGLGGTAGKIVCEQGMNIVFVAAEVTPWSKTGGLGDVLGGLPPAMAARGHRVMTIA 129
           S+++  +  TAGKIVCE+GM+++F+ AEV PWSKTGGLGDVLGGLPPA+AARGHRVMTI 
Sbjct: 68  SKRSSKVK-TAGKIVCEKGMSVIFIGAEVGPWSKTGGLGDVLGGLPPALAARGHRVMTIC 127

Query: 130 PRYDQYKDAWDTDVTVEIQVGDAILTVRFFHCYKRGVDRVFVDHPIFLEKVWGKTKSKLY 189
           PRYDQYKDAWDT V V+I+VGD +  VRFFHCYKRGVDRVFVDHPIFL KV GKT SK+Y
Sbjct: 128 PRYDQYKDAWDTCVVVQIKVGDKVENVRFFHCYKRGVDRVFVDHPIFLAKVVGKTGSKIY 187

Query: 190 GPNAGVDYDDNQLRFSLLCQAALLAPLVLNLNSSKYFSGPYGEEVVFVANDWHTALLPCY 249
           GP  GVDY+DNQLRFSLLCQAAL AP VLNLNSSKYFSGPYGE+VVFVANDWHTALLPCY
Sbjct: 188 GPITGVDYNDNQLRFSLLCQAALEAPQVLNLNSSKYFSGPYGEDVVFVANDWHTALLPCY 247

Query: 250 LKTMYQARGIYKSAKVAFCIHNIAYQGRFAFSDFSLLNLPDQFKSSFDFTDGYVKPVKGR 309
           LK+MYQ+RG+Y +AKV FCIHNIAYQGRFAF D+SLLNLP  FKSSFDF DGY KPVKGR
Sbjct: 248 LKSMYQSRGVYMNAKVVFCIHNIAYQGRFAFDDYSLLNLPISFKSSFDFMDGYEKPVKGR 307

Query: 310 KINWMKAGILESDRVLTVSPYYAQELVSGIERGVELDSILRLTTVTGILNGMDVQEWNPS 369
           KINWMKA ILE+ RVLTVSPYYAQEL+SG++RGVEL   LR+ TV+GI+NGMDVQEWNPS
Sbjct: 308 KINWMKAAILEAHRVLTVSPYYAQELISGVDRGVELHKYLRMKTVSGIINGMDVQEWNPS 367

Query: 370 TDKYIPIKFNAETVAEAKALLKEALQAEVGLPVDRNIPLIGFIGRLEEQKGSDILSAAIP 429
           TDKYI IK++  TV +AK L+KEALQA VGLPVDR++P+IGFIGRLEEQKGSDIL  AI 
Sbjct: 368 TDKYIDIKYDITTVTDAKPLIKEALQAAVGLPVDRDVPVIGFIGRLEEQKGSDILVEAIS 427

Query: 430 NFIHEDVQLVVLGTGKKYLEKQLEELEIKYPDKARGVAKFNVPLAHLINAAADFLMIPSR 489
            F+  +VQ+V+LGTGKK +E Q+ ELE K+P KA GVAKFNVPLAH+I A ADF+++PSR
Sbjct: 428 KFMGLNVQMVILGTGKKKMEAQILELEEKFPGKAVGVAKFNVPLAHMITAGADFIIVPSR 487

Query: 490 FEPCGLIQLQAMPYGTIPLVSTTGGLADTVIDGYTGLQMGAFSVECETVSPADAAAIAKT 549
           FEPCGLIQL AM YGT+P+V++TGGL DTV DGYTG  +G F+V+CE V P D  A AK 
Sbjct: 488 FEPCGLIQLHAMRYGTVPIVASTGGLVDTVKDGYTGFHIGRFNVKCEVVDPDDVIATAKA 547

Query: 550 VLRAVALFGTAAHKEMIQNCMAQDFSWKGPAKQWEKFLLGLGVENSQAGIEGEEIAPLAK 609
           V RAVA++GT+A +EM++NCM QDFSWKGPA+ WEK LL L V  S+AG EGEEIAPLAK
Sbjct: 548 VTRAVAVYGTSAMQEMVKNCMDQDFSWKGPARLWEKVLLSLNVAGSEAGTEGEEIAPLAK 607

Query: 610 ENVATP 615
           ENVATP
Sbjct: 608 ENVATP 610

BLAST of Cucsa.193420 vs. Swiss-Prot
Match: SSG1_IPOBA (Granule-bound starch synthase 1, chloroplastic/amyloplastic OS=Ipomoea batatas GN=WAXY PE=2 SV=2)

HSP 1 Score: 869.4 bits (2245), Expect = 2.4e-251
Identity = 432/613 (70.47%), Postives = 500/613 (81.57%), Query Frame = 1

Query: 4   MGAVSVSNFVSRSSLISHGATASETKMSLS-VGGKNQVETHKGLKSLNNLDVLRKRTPAS 63
           M  ++ S+FVS    +  GAT+ E+K+ L  +  ++Q  TH GL+ +N +D+L+ RT A 
Sbjct: 1   MATITASHFVSH---VCGGATSGESKVGLGQLALRSQAVTHNGLRPVNKIDMLQLRTSAK 60

Query: 64  AICRTVSRKTDGLGGTAGKIVC-EQGMNIVFVAAEVTPWSKTGGLGDVLGGLPPAMAARG 123
              +   R+ +G G  AG IVC +QGMN+VFV  EV PW KTGGLGDVLGGLPPA+AARG
Sbjct: 61  KPSKN-GRENEG-GMAAGTIVCKQQGMNLVFVGCEVGPWCKTGGLGDVLGGLPPALAARG 120

Query: 124 HRVMTIAPRYDQYKDAWDTDVTVEIQVGDAILTVRFFHCYKRGVDRVFVDHPIFLEKVWG 183
           HRVMT+ PRYDQYKDAWDT V VE+QVGD I  VRFFH YKRGVDRVFVDHP+FLEKVWG
Sbjct: 121 HRVMTVCPRYDQYKDAWDTCVVVELQVGDRIEPVRFFHSYKRGVDRVFVDHPMFLEKVWG 180

Query: 184 KTKSKLYGPNAGVDYDDNQLRFSLLCQAALLAPLVLNLNSSKYFSGPYGEEVVFVANDWH 243
           KT S LYGP AG DY DNQLRFSLLCQAAL AP VLNLNSS YFSGPYGE+VVFVANDWH
Sbjct: 181 KTGSMLYGPKAGKDYKDNQLRFSLLCQAALEAPRVLNLNSSNYFSGPYGEDVVFVANDWH 240

Query: 244 TALLPCYLKTMYQARGIYKSAKVAFCIHNIAYQGRFAFSDFSLLNLPDQFKSSFDFTDGY 303
           TALLPCYLKTMYQ+RGIY +AKVAFCIHNIAYQGRFAFSDFSLLNLPD++K SFDF DGY
Sbjct: 241 TALLPCYLKTMYQSRGIYMNAKVAFCIHNIAYQGRFAFSDFSLLNLPDEYKGSFDFIDGY 300

Query: 304 VKPVKGRKINWMKAGILESDRVLTVSPYYAQELVSGIERGVELDSILRLTTVTGILNGMD 363
            KPVKGRKINWMKAGI E+DRV TVSP YA+ELVS + +GVELD+ +R   +TGI NGMD
Sbjct: 301 DKPVKGRKINWMKAGIREADRVFTVSPNYAKELVSCVSKGVELDNHIRDCGITGICNGMD 360

Query: 364 VQEWNPSTDKYIPIKFNAETVAEAKALLKEALQAEVGLPVDRNIPLIGFIGRLEEQKGSD 423
            QEWNP+TDKY+ +K++  TV +AK LLKEALQA VGLPVDRNIPLIGFIGRLEEQKGSD
Sbjct: 361 TQEWNPATDKYLAVKYDITTVMQAKPLLKEALQAAVGLPVDRNIPLIGFIGRLEEQKGSD 420

Query: 424 ILSAAIPNFIHEDVQLVVLGTGKKYLEKQLEELEIKYPDKARGVAKFNVPLAHLINAAAD 483
           IL AAI  FI  DVQ+++LGTGKK  E+Q+E+LE+ YPDKARGVAKFNVPLAH+I A AD
Sbjct: 421 ILYAAISKFISMDVQILILGTGKKKFEQQIEQLEVMYPDKARGVAKFNVPLAHMITAGAD 480

Query: 484 FLMIPSRFEPCGLIQLQAMPYGTIPLVSTTGGLADTVIDGYTGLQMGAFSVECETVSPAD 543
           F++IPSRFEPCGLIQL AM YGT  + ++TGGL DTV +GYTG  MGAF+V+CETV P D
Sbjct: 481 FMLIPSRFEPCGLIQLHAMRYGTPCICASTGGLVDTVKEGYTGFHMGAFNVDCETVDPED 540

Query: 544 AAAIAKTVLRAVALFGTAAHKEMIQNCMAQDFSWKGPAKQWEKFLLGLGVENSQAGIEGE 603
              +  TV RA+A++GT A  EMI+NCM+Q+ SWKGPAK WE  LL LGV  S+ G+EG+
Sbjct: 541 VLKVITTVGRALAMYGTLAFTEMIKNCMSQELSWKGPAKNWETVLLSLGVAGSEPGVEGD 600

Query: 604 EIAPLAKENVATP 615
           EIAPLAKENVATP
Sbjct: 601 EIAPLAKENVATP 608

BLAST of Cucsa.193420 vs. TrEMBL
Match: G1JSF0_CUCMO (Starch synthase, chloroplastic/amyloplastic OS=Cucurbita moschata GN=GBSS PE=2 SV=1)

HSP 1 Score: 1103.6 bits (2853), Expect = 0.0e+00
Identity = 556/614 (90.55%), Postives = 581/614 (94.63%), Query Frame = 1

Query: 3   SMGAVSVSNFVSRSSLI-SHGATASETKMSLS-VGGKNQVETHKGLKSLNNLDVLRKRTP 62
           SMGAV+VSN VSRSSLI SHG + SETK SLS VG KNQVETHKGLKSLNNLD+LRK+TP
Sbjct: 8   SMGAVTVSNSVSRSSLILSHGHSVSETKKSLSPVGLKNQVETHKGLKSLNNLDMLRKKTP 67

Query: 63  ASAICRTVSRKTDGLGGTAGKIVCEQGMNIVFVAAEVTPWSKTGGLGDVLGGLPPAMAAR 122
           +SAICRT SRKTDG   TAGKIVCEQGMNIV VAAEVTPWSKTGGLGDVLGGLPPA+AAR
Sbjct: 68  SSAICRTASRKTDGNCRTAGKIVCEQGMNIVLVAAEVTPWSKTGGLGDVLGGLPPALAAR 127

Query: 123 GHRVMTIAPRYDQYKDAWDTDVTVEIQVGDAILTVRFFHCYKRGVDRVFVDHPIFLEKVW 182
           GHRVMTIAPRYDQYKDAWDT+VTVE+QVGD  L VRFFHCYKRGVDRVFVDHP+FLEKVW
Sbjct: 128 GHRVMTIAPRYDQYKDAWDTNVTVEMQVGDQTLNVRFFHCYKRGVDRVFVDHPMFLEKVW 187

Query: 183 GKTKSKLYGPNAGVDYDDNQLRFSLLCQAALLAPLVLNLNSSKYFSGPYGEEVVFVANDW 242
           GKTKSKLYGP AGVDY+DNQLRFSLLCQAALLAPLVLNLNSS+YFSGPYGE+VVFVANDW
Sbjct: 188 GKTKSKLYGPKAGVDYEDNQLRFSLLCQAALLAPLVLNLNSSEYFSGPYGEDVVFVANDW 247

Query: 243 HTALLPCYLKTMYQARGIYKSAKVAFCIHNIAYQGRFAFSDFSLLNLPDQFKSSFDFTDG 302
           HTALLPCYLK+MYQ RGIY SAKVAFCIHNIAYQGRF+FSDFS LNLPDQFKSSFDFTDG
Sbjct: 248 HTALLPCYLKSMYQTRGIYSSAKVAFCIHNIAYQGRFSFSDFSFLNLPDQFKSSFDFTDG 307

Query: 303 YVKPVKGRKINWMKAGILESDRVLTVSPYYAQELVSGIERGVELDSILRLTTVTGILNGM 362
           Y KPVKGRKIN MKAGILE+DRVLTVSPYYAQELVSG+ERGVELD+ LRLTTVTGILNGM
Sbjct: 308 YEKPVKGRKINRMKAGILEADRVLTVSPYYAQELVSGVERGVELDNSLRLTTVTGILNGM 367

Query: 363 DVQEWNPSTDKYIPIKFNAETVAEAKALLKEALQAEVGLPVDRNIPLIGFIGRLEEQKGS 422
           DVQEWNP+TDKYIPIKFNAE V EAKALLKEALQAEVGLPVDRNIPLIGFIGRLEEQKGS
Sbjct: 368 DVQEWNPATDKYIPIKFNAEAVTEAKALLKEALQAEVGLPVDRNIPLIGFIGRLEEQKGS 427

Query: 423 DILSAAIPNFIHEDVQLVVLGTGKKYLEKQLEELEIKYPDKARGVAKFNVPLAHLINAAA 482
           DILSAAIP FI E+VQLVVLGTGKKYLEKQLEELEIKYPDKARGVAKFNVPLAHLINAAA
Sbjct: 428 DILSAAIPTFIDENVQLVVLGTGKKYLEKQLEELEIKYPDKARGVAKFNVPLAHLINAAA 487

Query: 483 DFLMIPSRFEPCGLIQLQAMPYGTIPLVSTTGGLADTVIDGYTGLQMGAFSVECETVSPA 542
           DFL+IPSRFEPCGLIQLQAMPYGTIPLVSTTGGLADTVIDGYTGLQMGAFSVECETV PA
Sbjct: 488 DFLIIPSRFEPCGLIQLQAMPYGTIPLVSTTGGLADTVIDGYTGLQMGAFSVECETVDPA 547

Query: 543 DAAAIAKTVLRAVALFGTAAHKEMIQNCMAQDFSWKGPAKQWEKFLLGLGVENSQAGIEG 602
           DAAAIAKTVLRA+AL+G+AA +EM QNCMAQDFSWKGPAK WEK LL LGV+NSQ GIEG
Sbjct: 548 DAAAIAKTVLRALALYGSAALEEMRQNCMAQDFSWKGPAKLWEKLLLSLGVDNSQPGIEG 607

Query: 603 EEIAPLAKENVATP 615
           EEIAPLAKENVATP
Sbjct: 608 EEIAPLAKENVATP 621

BLAST of Cucsa.193420 vs. TrEMBL
Match: C0KH22_NELNU (Starch synthase, chloroplastic/amyloplastic OS=Nelumbo nucifera GN=Waxy PE=3 SV=1)

HSP 1 Score: 925.6 bits (2391), Expect = 3.1e-266
Identity = 459/621 (73.91%), Postives = 525/621 (84.54%), Query Frame = 1

Query: 4   MGAVSVSNFVSRSSLISHGA-TASETKMSLS-VGGKNQVETHKGLKSLNNLDVLRKRTPA 63
           M  V+ S FVS SSL+S+G  + S+ K +L+ +G +NQ  TH GL+S+NN+D L  R  A
Sbjct: 1   MATVTASKFVSTSSLVSYGGMSGSQPKTALAQIGLRNQTMTHHGLRSVNNVDKLNLRANA 60

Query: 64  SAI--------CRTVSRKTDGLGGTAGKIVCEQGMNIVFVAAEVTPWSKTGGLGDVLGGL 123
                      C+T + K   +      I C  GMN+VFV AEV PWSKTGGLGDVLGGL
Sbjct: 61  KTTTGQAKGKECKTRNAKERSV------IRCGSGMNLVFVGAEVGPWSKTGGLGDVLGGL 120

Query: 124 PPAMAARGHRVMTIAPRYDQYKDAWDTDVTVEIQVGDAILTVRFFHCYKRGVDRVFVDHP 183
           PPAMAA GHRVMT+APRYDQYKDAWDT V VEI+VGD I TVRFFHCYKRGVDRVFVDHP
Sbjct: 121 PPAMAANGHRVMTVAPRYDQYKDAWDTSVLVEIKVGDRIETVRFFHCYKRGVDRVFVDHP 180

Query: 184 IFLEKVWGKTKSKLYGPNAGVDYDDNQLRFSLLCQAALLAPLVLNLNSSKYFSGPYGEEV 243
           +FLEKVWGKT SK+YGP AG DY DNQLRFSLLCQAAL AP VLNLNSSK+FSGPYGE+V
Sbjct: 181 MFLEKVWGKTGSKIYGPMAGEDYSDNQLRFSLLCQAALEAPRVLNLNSSKHFSGPYGEDV 240

Query: 244 VFVANDWHTALLPCYLKTMYQARGIYKSAKVAFCIHNIAYQGRFAFSDFSLLNLPDQFKS 303
           VF+ NDWHTALLPCYLKTMY++RGIY++AKVAFCIHNIAYQGRF+FSDFSLLNLPD+FKS
Sbjct: 241 VFICNDWHTALLPCYLKTMYKSRGIYRNAKVAFCIHNIAYQGRFSFSDFSLLNLPDEFKS 300

Query: 304 SFDFTDGYVKPVKGRKINWMKAGILESDRVLTVSPYYAQELVSGIERGVELDSILRLTTV 363
           SFDF DGY KPVKGRKINWMKAGILESDRVLTVSPYYA+ELVSGIE+GVELD+I+R T +
Sbjct: 301 SFDFIDGYDKPVKGRKINWMKAGILESDRVLTVSPYYAEELVSGIEKGVELDNIIRKTGI 360

Query: 364 TGILNGMDVQEWNPSTDKYIPIKFNAETVAEAKALLKEALQAEVGLPVDRNIPLIGFIGR 423
           TGI+NG DVQEWNP+TDKYI +K++A TV +AK LLKEALQ+EVGLPVDRNIP+IGFIGR
Sbjct: 361 TGIVNGTDVQEWNPTTDKYISVKYDATTVMDAKPLLKEALQSEVGLPVDRNIPVIGFIGR 420

Query: 424 LEEQKGSDILSAAIPNFIHEDVQLVVLGTGKKYLEKQLEELEIKYPDKARGVAKFNVPLA 483
           LEEQKGSDIL+A+IP FI E+VQ++VLGTGKK  EKQLE+LEIKYPDKARGVAKFNVPLA
Sbjct: 421 LEEQKGSDILAASIPKFIGENVQIIVLGTGKKAFEKQLEQLEIKYPDKARGVAKFNVPLA 480

Query: 484 HLINAAADFLMIPSRFEPCGLIQLQAMPYGTIPLVSTTGGLADTVIDGYTGLQMGAFSVE 543
           H+I A ADFL+IPSRFEPCGLIQLQ MPYGTIPLVS+TGGL DTV +GYTG  MGAF+V+
Sbjct: 481 HMIIAGADFLLIPSRFEPCGLIQLQTMPYGTIPLVSSTGGLVDTVKEGYTGFHMGAFNVD 540

Query: 544 CETVSPADAAAIAKTVLRAVALFGTAAHKEMIQNCMAQDFSWKGPAKQWEKFLLGLGVEN 603
           C+ V PAD  A+A TV +A+  +GT+A  +MI+NCMAQ+FSWKGPAK+WE  LL L V  
Sbjct: 541 CDAVDPADVNAVATTVKKALKTYGTSAMIQMIKNCMAQNFSWKGPAKKWESILLSLEVAG 600

Query: 604 SQAGIEGEEIAPLAKENVATP 615
           S+ GI+GEEIAPLAKENVATP
Sbjct: 601 SEPGIDGEEIAPLAKENVATP 615

BLAST of Cucsa.193420 vs. TrEMBL
Match: A0A0D3N9U2_9ASTR (Starch synthase, chloroplastic/amyloplastic OS=Codonopsis pilosula PE=2 SV=1)

HSP 1 Score: 924.9 bits (2389), Expect = 5.3e-266
Identity = 450/614 (73.29%), Postives = 519/614 (84.53%), Query Frame = 1

Query: 4   MGAVSVSNFVSRSSLISHGATASETKMSLS-VGGKNQVETHKGLKSLNNLDVLRKRTPAS 63
           M  ++ S FVS ++        SET  +L+ +G +NQ  TH G +SLN +D+L+  T   
Sbjct: 1   MATITASKFVSHAN--GGAILGSETNTTLAKMGPRNQFMTHNGFRSLNAVDMLQMTTSPK 60

Query: 64  AICRTVSRKT--DGLGGTAGKIVCEQGMNIVFVAAEVTPWSKTGGLGDVLGGLPPAMAAR 123
           A  R V R     G     G I C+ GM++VF+ AEV PWSKTGGLGDVLGGLPPAMAA 
Sbjct: 61  ATARQVRRTAIKTGTKSGPGTITCKTGMSLVFLGAEVGPWSKTGGLGDVLGGLPPAMAAN 120

Query: 124 GHRVMTIAPRYDQYKDAWDTDVTVEIQVGDAILTVRFFHCYKRGVDRVFVDHPIFLEKVW 183
           GHRVMT++PRYDQYKDAWDT+V VEIQV D I TVRFFHCYKRGVDR+FVDHPIFLEKVW
Sbjct: 121 GHRVMTVSPRYDQYKDAWDTNVVVEIQVADKIETVRFFHCYKRGVDRIFVDHPIFLEKVW 180

Query: 184 GKTKSKLYGPNAGVDYDDNQLRFSLLCQAALLAPLVLNLNSSKYFSGPYGEEVVFVANDW 243
           GKT SK+YGPNAG DY DNQL+FSLLCQAAL+AP+VLNLNSSKYFSGPYGE+V+F+ANDW
Sbjct: 181 GKTGSKIYGPNAGEDYKDNQLKFSLLCQAALMAPMVLNLNSSKYFSGPYGEDVIFIANDW 240

Query: 244 HTALLPCYLKTMYQARGIYKSAKVAFCIHNIAYQGRFAFSDFSLLNLPDQFKSSFDFTDG 303
           HTALLPCYLKTMY++RG+YK+AKVAFCIHNIAYQGRF+FSDFSLLNLPDQ +SSFDF DG
Sbjct: 241 HTALLPCYLKTMYKSRGMYKNAKVAFCIHNIAYQGRFSFSDFSLLNLPDQVRSSFDFIDG 300

Query: 304 YVKPVKGRKINWMKAGILESDRVLTVSPYYAQELVSGIERGVELDSILRLTTVTGILNGM 363
           Y KPVKGRKINWMKAGILESDRV+TVSP+YA+ELVSG+E+GVELD+I+R T +TGI+NGM
Sbjct: 301 YEKPVKGRKINWMKAGILESDRVVTVSPHYAKELVSGVEKGVELDNIIRKTGITGIVNGM 360

Query: 364 DVQEWNPSTDKYIPIKFNAETVAEAKALLKEALQAEVGLPVDRNIPLIGFIGRLEEQKGS 423
           D+QEWNP TDKY+ +K++  TV +AK LLKEALQAEVGLPVDRNIPLIGFIGRLEEQKGS
Sbjct: 361 DIQEWNPLTDKYVDVKYDVTTVMDAKPLLKEALQAEVGLPVDRNIPLIGFIGRLEEQKGS 420

Query: 424 DILSAAIPNFIHEDVQLVVLGTGKKYLEKQLEELEIKYPDKARGVAKFNVPLAHLINAAA 483
           DIL+AAIP FI +DVQ+VVLGTGKKY+EKQLE+LEI YPDKARGVAKFNVPLAH I A A
Sbjct: 421 DILAAAIPKFIGQDVQIVVLGTGKKYMEKQLEQLEILYPDKARGVAKFNVPLAHKIIAGA 480

Query: 484 DFLMIPSRFEPCGLIQLQAMPYGTIPLVSTTGGLADTVIDGYTGLQMGAFSVECETVSPA 543
           DF++IPSRFEPCGLIQL +M YGT+P+V++TGGL DTV +GYTG Q+GAFSVEC+ V PA
Sbjct: 481 DFMIIPSRFEPCGLIQLHSMRYGTVPMVASTGGLVDTVEEGYTGFQIGAFSVECDAVEPA 540

Query: 544 DAAAIAKTVLRAVALFGTAAHKEMIQNCMAQDFSWKGPAKQWEKFLLGLGVENSQAGIEG 603
           D   I KTV RA+AL+GT A  EMI+NCM+QD SWKGPAK WE  LL LG   S+ GI+G
Sbjct: 541 DVLKIVKTVTRALALYGTLALNEMIKNCMSQDLSWKGPAKNWELMLLSLGAAGSEPGIDG 600

Query: 604 EEIAPLAKENVATP 615
           EEIAPLAKENVATP
Sbjct: 601 EEIAPLAKENVATP 612

BLAST of Cucsa.193420 vs. TrEMBL
Match: B5THH5_NELNU (Starch synthase, chloroplastic/amyloplastic OS=Nelumbo nucifera GN=Wx PE=2 SV=1)

HSP 1 Score: 921.0 bits (2379), Expect = 7.6e-265
Identity = 457/621 (73.59%), Postives = 523/621 (84.22%), Query Frame = 1

Query: 4   MGAVSVSNFVSRSSLISHGA-TASETKMSLS-VGGKNQVETHKGLKSLNNLDVLRKRTPA 63
           M  V+ S FVS SSL+S+G  + S+ K +L+ +G +NQ  TH GL+S+NN+D L  R  A
Sbjct: 1   MATVTASKFVSTSSLVSYGGMSGSQPKTALAQIGLRNQTMTHHGLRSVNNVDKLNLRANA 60

Query: 64  SAI--------CRTVSRKTDGLGGTAGKIVCEQGMNIVFVAAEVTPWSKTGGLGDVLGGL 123
                      C+T + K   +      I C  GMN+VFV AEV PWSKTGGLGDVLGGL
Sbjct: 61  KTTTGRAKGKECKTRNAKERSV------IRCGSGMNLVFVGAEVGPWSKTGGLGDVLGGL 120

Query: 124 PPAMAARGHRVMTIAPRYDQYKDAWDTDVTVEIQVGDAILTVRFFHCYKRGVDRVFVDHP 183
           PPAMAA GHRVMT+APRYDQYKDAWDT V VEI+VGD I TVRFFHCYKRGVDRVFVDHP
Sbjct: 121 PPAMAANGHRVMTVAPRYDQYKDAWDTSVLVEIKVGDRIETVRFFHCYKRGVDRVFVDHP 180

Query: 184 IFLEKVWGKTKSKLYGPNAGVDYDDNQLRFSLLCQAALLAPLVLNLNSSKYFSGPYGEEV 243
           +FLEKVWGKT SK+YGP +G DY DNQLRFSLLCQAAL AP VLNLNSSK FSGPYGE+V
Sbjct: 181 MFLEKVWGKTGSKIYGPMSGEDYSDNQLRFSLLCQAALEAPRVLNLNSSKPFSGPYGEDV 240

Query: 244 VFVANDWHTALLPCYLKTMYQARGIYKSAKVAFCIHNIAYQGRFAFSDFSLLNLPDQFKS 303
           VF+ NDWHTALLPCYLKTMY++RGIY++AKVAFCIHNIAYQGRF+FSDFSLLNLPD+FKS
Sbjct: 241 VFICNDWHTALLPCYLKTMYKSRGIYRNAKVAFCIHNIAYQGRFSFSDFSLLNLPDEFKS 300

Query: 304 SFDFTDGYVKPVKGRKINWMKAGILESDRVLTVSPYYAQELVSGIERGVELDSILRLTTV 363
           SFDF DGY KPVKGRKINWMKAGILESDRVLTVSPYYA+EL SGIE+GVELD+I+R T +
Sbjct: 301 SFDFIDGYDKPVKGRKINWMKAGILESDRVLTVSPYYAEELASGIEKGVELDNIIRKTGI 360

Query: 364 TGILNGMDVQEWNPSTDKYIPIKFNAETVAEAKALLKEALQAEVGLPVDRNIPLIGFIGR 423
           TGI+NG DVQEWNP+TDKYI +K++A TV +AK LLKEALQ+EVGLPVDRNIP+IGFIGR
Sbjct: 361 TGIVNGTDVQEWNPTTDKYISVKYDATTVMDAKPLLKEALQSEVGLPVDRNIPVIGFIGR 420

Query: 424 LEEQKGSDILSAAIPNFIHEDVQLVVLGTGKKYLEKQLEELEIKYPDKARGVAKFNVPLA 483
           LEEQKGSDIL+A+IP FI E+VQ++VLGTGKK  EKQLE+LEIKYPDKARGVAKFNVPLA
Sbjct: 421 LEEQKGSDILAASIPKFIGENVQIIVLGTGKKAFEKQLEQLEIKYPDKARGVAKFNVPLA 480

Query: 484 HLINAAADFLMIPSRFEPCGLIQLQAMPYGTIPLVSTTGGLADTVIDGYTGLQMGAFSVE 543
           H+I A ADFL+IPSRFEPCGLIQLQ MPYGTIPLVS+TGGL DTV +GYTG  MGAF+V+
Sbjct: 481 HMIIAGADFLLIPSRFEPCGLIQLQTMPYGTIPLVSSTGGLVDTVKEGYTGFHMGAFNVD 540

Query: 544 CETVSPADAAAIAKTVLRAVALFGTAAHKEMIQNCMAQDFSWKGPAKQWEKFLLGLGVEN 603
           C+ V PAD  A+A TV +A+  +GT+A  +MI+NCMAQ+FSWKGPAK+WE  LL L V  
Sbjct: 541 CDAVDPADVNAVATTVKKALKTYGTSAMIQMIKNCMAQNFSWKGPAKKWESILLSLEVAG 600

Query: 604 SQAGIEGEEIAPLAKENVATP 615
           S+ GI+GEEIAPLAKENVATP
Sbjct: 601 SEPGIDGEEIAPLAKENVATP 615

BLAST of Cucsa.193420 vs. TrEMBL
Match: B2LUN6_MALDO (Starch synthase, chloroplastic/amyloplastic OS=Malus domestica GN=GBSSIb PE=2 SV=1)

HSP 1 Score: 916.8 bits (2368), Expect = 1.4e-263
Identity = 448/619 (72.37%), Postives = 522/619 (84.33%), Query Frame = 1

Query: 4   MGAVSVSNFVSRSSLISHGATASET-------KMSLSVGGKNQVETHKGLKSLNNLDVLR 63
           M  ++ S+F S SS +S+GA +S +       KMS       Q  TH GL++LN++D LR
Sbjct: 1   MATITASSFASTSSHVSYGAVSSGSDPKTGFKKMSFG----KQTLTHNGLRALNSVDELR 60

Query: 64  KRTPASAICRTVSRKT-DGLGGTAGKIVCEQGMNIVFVAAEVTPWSKTGGLGDVLGGLPP 123
            R  A+++ R    KT +    T+G IVC  GMN+VF+  EV PWSKTGGLGDVLGGLPP
Sbjct: 61  VRIMANSVARQTRGKTVNSTRKTSGVIVCGSGMNLVFLGTEVGPWSKTGGLGDVLGGLPP 120

Query: 124 AMAARGHRVMTIAPRYDQYKDAWDTDVTVEIQVGDAILTVRFFHCYKRGVDRVFVDHPIF 183
           AMAA GHRVMTI+PRYDQYKDAWDT+VTVE++VGD   TVRFFHCYKRGVDRVFVDHP+F
Sbjct: 121 AMAANGHRVMTISPRYDQYKDAWDTEVTVELKVGDKTETVRFFHCYKRGVDRVFVDHPLF 180

Query: 184 LEKVWGKTKSKLYGPNAGVDYDDNQLRFSLLCQAALLAPLVLNLNSSKYFSGPYGEEVVF 243
           LEKVWGKT SK+YGP AGVD+ DNQLRFSLLCQAAL+AP VLNLNSSKYFSGPYGEEVVF
Sbjct: 181 LEKVWGKTASKIYGPVAGVDFKDNQLRFSLLCQAALVAPRVLNLNSSKYFSGPYGEEVVF 240

Query: 244 VANDWHTALLPCYLKTMYQARGIYKSAKVAFCIHNIAYQGRFAFSDFSLLNLPDQFKSSF 303
           +ANDWHTALLPCYLK +Y+ +GIYK+AKVAFCIHNIAYQGRFAF+DF+LLNLP++FKSSF
Sbjct: 241 IANDWHTALLPCYLKAIYKPKGIYKTAKVAFCIHNIAYQGRFAFADFALLNLPNEFKSSF 300

Query: 304 DFTDGYVKPVKGRKINWMKAGILESDRVLTVSPYYAQELVSGIERGVELDSILRLTTVTG 363
           DF DGY KPVKGRKINWMKAGILESD+VLTVSPYYA+ELVS +E+GVELD+ILR + + G
Sbjct: 301 DFIDGYNKPVKGRKINWMKAGILESDKVLTVSPYYAEELVSSVEKGVELDNILRKSRIQG 360

Query: 364 ILNGMDVQEWNPSTDKYIPIKFNAETVAEAKALLKEALQAEVGLPVDRNIPLIGFIGRLE 423
           I+NGMDVQEWNP TDKY  +K++A TVA+AK LLKEALQAEVGLPVDR+IP+IGFIGRLE
Sbjct: 361 IVNGMDVQEWNPVTDKYTTVKYDASTVADAKPLLKEALQAEVGLPVDRDIPVIGFIGRLE 420

Query: 424 EQKGSDILSAAIPNFIHEDVQLVVLGTGKKYLEKQLEELEIKYPDKARGVAKFNVPLAHL 483
           EQKGSDIL  AIP+FI E+VQ++VLGTGKK +EKQLE+LE +YPDKARG+AKFNVPLAH+
Sbjct: 421 EQKGSDILIEAIPHFIKENVQIIVLGTGKKPMEKQLEQLETEYPDKARGIAKFNVPLAHM 480

Query: 484 INAAADFLMIPSRFEPCGLIQLQAMPYGTIPLVSTTGGLADTVIDGYTGLQMGAFSVECE 543
           I A ADF+++PSRFEPCGLIQL AM YGT+P+V++TGGL DTV +G+TG  MGAF+VECE
Sbjct: 481 ITAGADFMLVPSRFEPCGLIQLHAMRYGTVPIVASTGGLVDTVKEGFTGFHMGAFNVECE 540

Query: 544 TVSPADAAAIAKTVLRAVALFGTAAHKEMIQNCMAQDFSWKGPAKQWEKFLLGLGVENSQ 603
            V P D  AIA TV RA+  +GT A  E+I NCMAQD SWKGPAK+WE+ LL LGV NS+
Sbjct: 541 VVDPVDVQAIATTVTRALGSYGTPAFTEIISNCMAQDLSWKGPAKKWEEVLLSLGVANSE 600

Query: 604 AGIEGEEIAPLAKENVATP 615
            GIEGEEIAPLAKENVATP
Sbjct: 601 LGIEGEEIAPLAKENVATP 615

BLAST of Cucsa.193420 vs. TAIR10
Match: AT1G32900.1 (AT1G32900.1 UDP-Glycosyltransferase superfamily protein)

HSP 1 Score: 878.6 bits (2269), Expect = 2.2e-255
Identity = 432/606 (71.29%), Postives = 506/606 (83.50%), Query Frame = 1

Query: 10  SNFVSRSSLIS-HGATASETKMSLSVGGKNQVETHKGLKSLNNLDVLRKRTPASAICRTV 69
           SNFVSR+SL + HGA++      +++  K Q  TH GL+S N +D L++R+ A  +    
Sbjct: 8   SNFVSRTSLFNNHGASSCSDVAQITL--KGQSLTHCGLRSFNMVDNLQRRSQAKPVSAKS 67

Query: 70  SRKTDGLGGTAGKIVCEQGMNIVFVAAEVTPWSKTGGLGDVLGGLPPAMAARGHRVMTIA 129
           S+++  +  TAGKIVCE+GM+++F+ AEV PWSKTGGLGDVLGGLPPA+AARGHRVMTI 
Sbjct: 68  SKRSSKVK-TAGKIVCEKGMSVIFIGAEVGPWSKTGGLGDVLGGLPPALAARGHRVMTIC 127

Query: 130 PRYDQYKDAWDTDVTVEIQVGDAILTVRFFHCYKRGVDRVFVDHPIFLEKVWGKTKSKLY 189
           PRYDQYKDAWDT V V+I+VGD +  VRFFHCYKRGVDRVFVDHPIFL KV GKT SK+Y
Sbjct: 128 PRYDQYKDAWDTCVVVQIKVGDKVENVRFFHCYKRGVDRVFVDHPIFLAKVVGKTGSKIY 187

Query: 190 GPNAGVDYDDNQLRFSLLCQAALLAPLVLNLNSSKYFSGPYGEEVVFVANDWHTALLPCY 249
           GP  GVDY+DNQLRFSLLCQAAL AP VLNLNSSKYFSGPYGE+VVFVANDWHTALLPCY
Sbjct: 188 GPITGVDYNDNQLRFSLLCQAALEAPQVLNLNSSKYFSGPYGEDVVFVANDWHTALLPCY 247

Query: 250 LKTMYQARGIYKSAKVAFCIHNIAYQGRFAFSDFSLLNLPDQFKSSFDFTDGYVKPVKGR 309
           LK+MYQ+RG+Y +AKV FCIHNIAYQGRFAF D+SLLNLP  FKSSFDF DGY KPVKGR
Sbjct: 248 LKSMYQSRGVYMNAKVVFCIHNIAYQGRFAFDDYSLLNLPISFKSSFDFMDGYEKPVKGR 307

Query: 310 KINWMKAGILESDRVLTVSPYYAQELVSGIERGVELDSILRLTTVTGILNGMDVQEWNPS 369
           KINWMKA ILE+ RVLTVSPYYAQEL+SG++RGVEL   LR+ TV+GI+NGMDVQEWNPS
Sbjct: 308 KINWMKAAILEAHRVLTVSPYYAQELISGVDRGVELHKYLRMKTVSGIINGMDVQEWNPS 367

Query: 370 TDKYIPIKFNAETVAEAKALLKEALQAEVGLPVDRNIPLIGFIGRLEEQKGSDILSAAIP 429
           TDKYI IK++  TV +AK L+KEALQA VGLPVDR++P+IGFIGRLEEQKGSDIL  AI 
Sbjct: 368 TDKYIDIKYDITTVTDAKPLIKEALQAAVGLPVDRDVPVIGFIGRLEEQKGSDILVEAIS 427

Query: 430 NFIHEDVQLVVLGTGKKYLEKQLEELEIKYPDKARGVAKFNVPLAHLINAAADFLMIPSR 489
            F+  +VQ+V+LGTGKK +E Q+ ELE K+P KA GVAKFNVPLAH+I A ADF+++PSR
Sbjct: 428 KFMGLNVQMVILGTGKKKMEAQILELEEKFPGKAVGVAKFNVPLAHMITAGADFIIVPSR 487

Query: 490 FEPCGLIQLQAMPYGTIPLVSTTGGLADTVIDGYTGLQMGAFSVECETVSPADAAAIAKT 549
           FEPCGLIQL AM YGT+P+V++TGGL DTV DGYTG  +G F+V+CE V P D  A AK 
Sbjct: 488 FEPCGLIQLHAMRYGTVPIVASTGGLVDTVKDGYTGFHIGRFNVKCEVVDPDDVIATAKA 547

Query: 550 VLRAVALFGTAAHKEMIQNCMAQDFSWKGPAKQWEKFLLGLGVENSQAGIEGEEIAPLAK 609
           V RAVA++GT+A +EM++NCM QDFSWKGPA+ WEK LL L V  S+AG EGEEIAPLAK
Sbjct: 548 VTRAVAVYGTSAMQEMVKNCMDQDFSWKGPARLWEKVLLSLNVAGSEAGTEGEEIAPLAK 607

Query: 610 ENVATP 615
           ENVATP
Sbjct: 608 ENVATP 610

BLAST of Cucsa.193420 vs. TAIR10
Match: AT3G01180.1 (AT3G01180.1 starch synthase 2)

HSP 1 Score: 357.8 bits (917), Expect = 1.3e-98
Identity = 218/512 (42.58%), Postives = 291/512 (56.84%), Query Frame = 1

Query: 88  MNIVFVAAEVTPWSKTGGLGDVLGGLPPAMAARGHRVMTIAPRYDQYKDAWDTDVTVEIQ 147
           MN++ VAAE  P+SKTGGLGDV G LP ++A RGHRVM + PRY +Y +A D  V    +
Sbjct: 301 MNVILVAAECAPFSKTGGLGDVAGALPKSLARRGHRVMVVVPRYAEYAEAKDLGVRKRYK 360

Query: 148 VGDAILTVRFFHCYKRGVDRVFVDHPIFLEKVWGKTKSKLYGPNAGVDYDDNQLRFSLLC 207
           V    + V +FH +  GVD VF+D P F         + +YG N      D   R  L C
Sbjct: 361 VAGQDMEVMYFHAFIDGVDFVFIDSPEFRH-----LSNNIYGGNRL----DILKRMVLFC 420

Query: 208 QAALLAPLVLNLNSSKYFSGPYGEEVVFVANDWHTALLPCYLKTMYQARGIYKSAKVAFC 267
           +AA+  P  +      Y  G     + F+ANDWHTALLP YLK  Y+  GI K  +    
Sbjct: 421 KAAVEVPWYVPCGGVCYGDG----NLAFIANDWHTALLPVYLKAYYRDHGIMKYTRSVLV 480

Query: 268 IHNIAYQGRFAFSDFSLLNLPDQFKSSFDFTDGYVKPVKGRKINWMKAGILESDRVLTVS 327
           IHNIA+QGR    DFS ++LP  +  SF   D    PV G   N   AG+  +DRVLTVS
Sbjct: 481 IHNIAHQGRGPVDDFSYVDLPSHYLDSFKLYD----PVGGEHFNIFAAGLKAADRVLTVS 540

Query: 328 PYYAQELVSGIERGVELDSILRLTT--VTGILNGMDVQEWNPSTDKYIP----IKFNAET 387
             Y+ E V  +E G  L +I+        GI+NG+D QEWNP  D Y+       ++ E 
Sbjct: 541 HGYSWE-VKTLEGGWGLHNIINENDWKFRGIVNGIDTQEWNPEFDTYLHSDDYTNYSLEN 600

Query: 388 VAEAKALLKEALQAEVGLPVDRNIPLIGFIGRLEEQKGSDILSAAIPNFIHEDVQLVVLG 447
           +   K   K ALQ E+GLPV  ++PLIGFIGRL+ QKG D+++ A+P  + +DVQLV+LG
Sbjct: 601 LHIGKPQCKAALQKELGLPVRPDVPLIGFIGRLDHQKGVDLIAEAVPWMMSQDVQLVMLG 660

Query: 448 TGKKYLEKQLEELEIKYPDKARGVAKFNVPLAHLINAAADFLMIPSRFEPCGLIQLQAMP 507
           TG+  LE+ L ++E +Y DKARG   F+V  AH I A AD L++PSRFEPCGL QL AM 
Sbjct: 661 TGRPDLEEVLRQMEHQYRDKARGWVGFSVKTAHRITAGADILLMPSRFEPCGLNQLYAMN 720

Query: 508 YGTIPLVSTTGGLADTV--IDGYTGLQMGAFSVECETVSPADAAAIAKTVLRAVALFGTA 567
           YGTIP+V   GGL DTV   D Y+   +G       T   A+A  +   +   +  +   
Sbjct: 721 YGTIPVVHAVGGLRDTVQQFDPYSETGLG------WTFDSAEAGKLIHALGNCLLTY--R 780

Query: 568 AHKE----MIQNCMAQDFSWKGPAKQWEKFLL 588
            +KE    + +  M QD SW   A+++E+ L+
Sbjct: 781 EYKESWEGLQRRGMTQDLSWDNAAEKYEEVLV 786

BLAST of Cucsa.193420 vs. TAIR10
Match: AT5G24300.1 (AT5G24300.1 Glycogen/starch synthases, ADP-glucose type)

HSP 1 Score: 357.1 bits (915), Expect = 2.2e-98
Identity = 216/538 (40.15%), Postives = 304/538 (56.51%), Query Frame = 1

Query: 66  RTVSRKTDGLGGTAGKIVCEQGMNIVFVAAEVTPWSKTGGLGDVLGGLPPAMAARGHRVM 125
           R V      +G  +GK   E   N+VFV +E  P+SKTGGLGDV G LP A+A RGHRVM
Sbjct: 122 RAVEEGAAEVGIPSGK--AEVVNNLVFVTSEAAPYSKTGGLGDVCGSLPIALAGRGHRVM 181

Query: 126 TIAPRY-------DQYKDAWDTDVTVEIQVGDAILTVRFFHCYKRGVDRVFVDHPIFLEK 185
            I+PRY         Y  A D  + V +        V F+H Y+ GVD VFVDH  +   
Sbjct: 182 VISPRYLNGTAADKNYARAKDLGIRVTVNCFGGSQEVSFYHEYRDGVDWVFVDHKSY--- 241

Query: 186 VWGKTKSKLYGPNAGVDYDDNQLRFSLLCQAALLAPLVLNLNSSKYFSGPYGEEVVFVAN 245
                    YG + G  + DNQ RF+LLC AA  APLVL L    Y     GE+ +F+ N
Sbjct: 242 ---HRPGNPYGDSKGA-FGDNQFRFTLLCHAACEAPLVLPLGGFTY-----GEKSLFLVN 301

Query: 246 DWHTALLPCYLKTMYQARGIYKSAKVAFCIHNIAYQGRFAFSDFSLLNLPDQFKSSFDFT 305
           DWH  L+P  L   Y+  G+YK A+    IHN+A+QG    + ++ L LP ++  +    
Sbjct: 302 DWHAGLVPILLAAKYRPYGVYKDARSILIIHNLAHQGVEPAATYTNLGLPSEWYGAV--- 361

Query: 306 DGYVKPV--------KGRKINWMKAGILESDRVLTVSPYYAQELVSGIERGVELDSIL-- 365
            G+V P          G  +N +K  I+ SDR++TVS  YA E+ + +E G  L  +L  
Sbjct: 362 -GWVFPTWARTHALDTGEAVNVLKGAIVTSDRIITVSQGYAWEITT-VEGGYGLQDLLSS 421

Query: 366 RLTTVTGILNGMDVQEWNPSTDKYIPIKFNAETVAEAKALLKEALQAEVGLPVDRNIPLI 425
           R + + GI NG++V EWNPSTD++IP  ++A+ V+E K   K ALQ E+GLP+    P+I
Sbjct: 422 RKSVINGITNGINVDEWNPSTDEHIPFHYSADDVSE-KIKCKMALQKELGLPIRPECPMI 481

Query: 426 GFIGRLEEQKGSDILSAAIPNFIHEDVQLVVLGTGKKYLEKQLEELEIKYPDKARGVAKF 485
           GFIGRL+ QKG D++  A P+ + +D+Q V+LG+G    E  +  +E  Y DK RG   F
Sbjct: 482 GFIGRLDYQKGIDLIQTAGPDLMVDDIQFVMLGSGDPKYESWMRSMEETYRDKFRGWVGF 541

Query: 486 NVPLAHLINAAADFLMIPSRFEPCGLIQLQAMPYGTIPLVSTTGGLADTVIDGYTGLQMG 545
           NVP++H I A  D L++PSRFEPCGL QL AM YGTIP+V  TGGL DTV +     + G
Sbjct: 542 NVPISHRITAGCDILLMPSRFEPCGLNQLYAMRYGTIPVVHGTGGLRDTVENFNPYAEGG 601

Query: 546 AFSVECETVSPADAAAIAKTVLRAVALFG--TAAHKEMIQNCMAQDFSWKGPAKQWEK 585
           A +      +P    ++   +  A A +     + + +++  M +++SW+  A Q+E+
Sbjct: 602 AGTGTGWVFTPLSKDSMVSALRLAAATYREYKQSWEGLMRRGMTRNYSWENAAVQYEQ 639

BLAST of Cucsa.193420 vs. TAIR10
Match: AT4G18240.1 (AT4G18240.1 starch synthase 4)

HSP 1 Score: 207.6 bits (527), Expect = 2.2e-53
Identity = 170/518 (32.82%), Postives = 252/518 (48.65%), Query Frame = 1

Query: 87   GMNIVFVAAEVTPWSKTGGLGDVLGGLPPAMAARGHRVMTIAPRYD--QY---KDAWDTD 146
            G+ +V +AAE+ P +K GGLGDV+ GL  A+  +GH V  I P+YD  QY   +D    D
Sbjct: 541  GLYVVHIAAEMAPVAKVGGLGDVVAGLGKALQRKGHLVEIILPKYDCMQYDRVRDLRALD 600

Query: 147  VTVEIQVGDAILTVRFFHCYKRGVDRVFVDHPIFLEKVWGKTKSKLYGPNAGVDYDDNQL 206
              VE      +   + +     G+   F++     +  W   + + YG        D+  
Sbjct: 601  TVVESYFDGKLYKNKIWIGTVEGLPVHFIEPQHPSKFFW---RGQFYGEQ------DDFR 660

Query: 207  RFSLLCQAALLAPLVLNLNSSKYFSGPYGEEVVFVANDWHTALLPCYLKTMYQARGIYKS 266
            RFS   +AAL     L L S K       +  +   +DW TA +      +Y  +G+  S
Sbjct: 661  RFSYFSRAAL----ELLLQSGK-------KPDIIHCHDWQTAFVAPLYWDLYAPKGL-DS 720

Query: 267  AKVAFCIHNIAYQGRFAFS-------DFSLLNLPDQFKSSFDFTDGYVKPVKGRKINWMK 326
            A++ F  HN  YQG  + S       D + LN PD+ +              G ++N +K
Sbjct: 721  ARICFTCHNFEYQGTASASELGSCGLDVNQLNRPDRMQDHSS----------GDRVNPVK 780

Query: 327  AGILESDRVLTVSPYYAQELVSGIERGVELDSILRLTT--VTGILNGMDVQEWNPSTDKY 386
              I+ S+ V TVSP YAQE V   E G  L S L   +    GILNG+D   WNP+TD +
Sbjct: 781  GAIIFSNIVTTVSPTYAQE-VRTAEGGKGLHSTLNFHSKKFIGILNGIDTDSWNPATDPF 840

Query: 387  IPIKFNAETVAEAKALLKEALQAEVGLP-VDRNIPLIGFIGRLEEQKGSDILSAAIPNFI 446
            +  +FNA+ + + K   K AL+ ++GL   +   PL+G I RL  QKG  ++  AI   +
Sbjct: 841  LKAQFNAKDL-QGKEENKHALRKQLGLSSAESRRPLVGCITRLVPQKGVHLIRHAIYRTL 900

Query: 447  HEDVQLVVLGTGK-KYLEKQLEELE--IKYPDKARGVAKFNVPLAHLINAAADFLMIPSR 506
                Q V+LG+    +++++ E +E   K  D  R + K++  L+H I AA+D  +IPS 
Sbjct: 901  ELGGQFVLLGSSPVPHIQREFEGIEQQFKSHDHVRLLLKYDEALSHTIYAASDLFIIPSI 960

Query: 507  FEPCGLIQLQAMPYGTIPLVSTTGGLADTVIDGYTGLQMGAFSVECETVSPADAAAIAKT 566
            FEPCGL Q+ AM YG+IP+   TGGL D+V D         F     T   AD       
Sbjct: 961  FEPCGLTQMIAMRYGSIPIARKTGGLNDSVFDIDDDTIPTQFQ-NGFTFQTADEQGFNYA 1020

Query: 567  VLRAVALFGTAAHK--EMIQNCMAQDFSWKGPAKQWEK 585
            + RA   +     K   +++  M+ DFSW   A Q+E+
Sbjct: 1021 LERAFNHYKKDEEKWMRLVEKVMSIDFSWGSSATQYEE 1024

BLAST of Cucsa.193420 vs. TAIR10
Match: AT1G11720.2 (AT1G11720.2 starch synthase 3)

HSP 1 Score: 152.5 bits (384), Expect = 8.3e-37
Identity = 96/279 (34.41%), Postives = 147/279 (52.69%), Query Frame = 1

Query: 320  SDRVLTVSPYYAQELVSGIERGVELDSIL--RLTTVTGILNGMDVQEWNPSTDKYIPIKF 379
            +D+  TVSP YA+E+          +S++   L    GI+NG+D   W+P  D +IP+ +
Sbjct: 820  ADKATTVSPTYAKEVAG--------NSVISAHLYKFHGIINGIDPDIWDPYNDNFIPVPY 879

Query: 380  NAETVAEAKALLKEALQAEVGLPVDRNIPLIGFIGRLEEQKGSDILSAAIPNFIHEDVQL 439
             +E V E K   KE LQ  +GL    + P++G I RL  QKG  ++  AI   +  + Q+
Sbjct: 880  TSENVVEGKRAAKEELQNRLGLK-SADFPVVGIITRLTHQKGIHLIKHAIWRTLERNGQV 939

Query: 440  VVLGTG-----KKYLEKQLEELEIKYPDKARGVAKFNVPLAHLINAAADFLMIPSRFEPC 499
            V+LG+      +        +L   + D+AR V  ++ PL+HLI A ADF+++PS FEPC
Sbjct: 940  VLLGSAPDPRIQNDFVNLANQLHSSHGDRARLVLTYDEPLSHLIYAGADFILVPSIFEPC 999

Query: 500  GLIQLQAMPYGTIPLVSTTGGLADTVIDGYTGLQMGAFSVECETVSP-------ADAAAI 559
            GL QL AM YG +P+V  TGGL DTV D    +       + + + P       ADA  +
Sbjct: 1000 GLTQLIAMRYGAVPVVRKTGGLFDTVFD----VDHDKERAQAQVLEPNGFSFDGADAPGV 1059

Query: 560  AKTVLRAVALF--GTAAHKEMIQNCMAQDFSWKGPAKQW 583
               + RA++ +  G      + +  M QD+SW  PA ++
Sbjct: 1060 DYALNRAISAWYDGREWFNSLCKTVMEQDWSWNRPALEY 1085

BLAST of Cucsa.193420 vs. NCBI nr
Match: gi|449456273|ref|XP_004145874.1| (PREDICTED: granule-bound starch synthase 1, chloroplastic/amyloplastic [Cucumis sativus])

HSP 1 Score: 1224.9 bits (3168), Expect = 0.0e+00
Identity = 614/614 (100.00%), Postives = 614/614 (100.00%), Query Frame = 1

Query: 1   MASMGAVSVSNFVSRSSLISHGATASETKMSLSVGGKNQVETHKGLKSLNNLDVLRKRTP 60
           MASMGAVSVSNFVSRSSLISHGATASETKMSLSVGGKNQVETHKGLKSLNNLDVLRKRTP
Sbjct: 1   MASMGAVSVSNFVSRSSLISHGATASETKMSLSVGGKNQVETHKGLKSLNNLDVLRKRTP 60

Query: 61  ASAICRTVSRKTDGLGGTAGKIVCEQGMNIVFVAAEVTPWSKTGGLGDVLGGLPPAMAAR 120
           ASAICRTVSRKTDGLGGTAGKIVCEQGMNIVFVAAEVTPWSKTGGLGDVLGGLPPAMAAR
Sbjct: 61  ASAICRTVSRKTDGLGGTAGKIVCEQGMNIVFVAAEVTPWSKTGGLGDVLGGLPPAMAAR 120

Query: 121 GHRVMTIAPRYDQYKDAWDTDVTVEIQVGDAILTVRFFHCYKRGVDRVFVDHPIFLEKVW 180
           GHRVMTIAPRYDQYKDAWDTDVTVEIQVGDAILTVRFFHCYKRGVDRVFVDHPIFLEKVW
Sbjct: 121 GHRVMTIAPRYDQYKDAWDTDVTVEIQVGDAILTVRFFHCYKRGVDRVFVDHPIFLEKVW 180

Query: 181 GKTKSKLYGPNAGVDYDDNQLRFSLLCQAALLAPLVLNLNSSKYFSGPYGEEVVFVANDW 240
           GKTKSKLYGPNAGVDYDDNQLRFSLLCQAALLAPLVLNLNSSKYFSGPYGEEVVFVANDW
Sbjct: 181 GKTKSKLYGPNAGVDYDDNQLRFSLLCQAALLAPLVLNLNSSKYFSGPYGEEVVFVANDW 240

Query: 241 HTALLPCYLKTMYQARGIYKSAKVAFCIHNIAYQGRFAFSDFSLLNLPDQFKSSFDFTDG 300
           HTALLPCYLKTMYQARGIYKSAKVAFCIHNIAYQGRFAFSDFSLLNLPDQFKSSFDFTDG
Sbjct: 241 HTALLPCYLKTMYQARGIYKSAKVAFCIHNIAYQGRFAFSDFSLLNLPDQFKSSFDFTDG 300

Query: 301 YVKPVKGRKINWMKAGILESDRVLTVSPYYAQELVSGIERGVELDSILRLTTVTGILNGM 360
           YVKPVKGRKINWMKAGILESDRVLTVSPYYAQELVSGIERGVELDSILRLTTVTGILNGM
Sbjct: 301 YVKPVKGRKINWMKAGILESDRVLTVSPYYAQELVSGIERGVELDSILRLTTVTGILNGM 360

Query: 361 DVQEWNPSTDKYIPIKFNAETVAEAKALLKEALQAEVGLPVDRNIPLIGFIGRLEEQKGS 420
           DVQEWNPSTDKYIPIKFNAETVAEAKALLKEALQAEVGLPVDRNIPLIGFIGRLEEQKGS
Sbjct: 361 DVQEWNPSTDKYIPIKFNAETVAEAKALLKEALQAEVGLPVDRNIPLIGFIGRLEEQKGS 420

Query: 421 DILSAAIPNFIHEDVQLVVLGTGKKYLEKQLEELEIKYPDKARGVAKFNVPLAHLINAAA 480
           DILSAAIPNFIHEDVQLVVLGTGKKYLEKQLEELEIKYPDKARGVAKFNVPLAHLINAAA
Sbjct: 421 DILSAAIPNFIHEDVQLVVLGTGKKYLEKQLEELEIKYPDKARGVAKFNVPLAHLINAAA 480

Query: 481 DFLMIPSRFEPCGLIQLQAMPYGTIPLVSTTGGLADTVIDGYTGLQMGAFSVECETVSPA 540
           DFLMIPSRFEPCGLIQLQAMPYGTIPLVSTTGGLADTVIDGYTGLQMGAFSVECETVSPA
Sbjct: 481 DFLMIPSRFEPCGLIQLQAMPYGTIPLVSTTGGLADTVIDGYTGLQMGAFSVECETVSPA 540

Query: 541 DAAAIAKTVLRAVALFGTAAHKEMIQNCMAQDFSWKGPAKQWEKFLLGLGVENSQAGIEG 600
           DAAAIAKTVLRAVALFGTAAHKEMIQNCMAQDFSWKGPAKQWEKFLLGLGVENSQAGIEG
Sbjct: 541 DAAAIAKTVLRAVALFGTAAHKEMIQNCMAQDFSWKGPAKQWEKFLLGLGVENSQAGIEG 600

Query: 601 EEIAPLAKENVATP 615
           EEIAPLAKENVATP
Sbjct: 601 EEIAPLAKENVATP 614

BLAST of Cucsa.193420 vs. NCBI nr
Match: gi|659074162|ref|XP_008437455.1| (PREDICTED: granule-bound starch synthase 1, chloroplastic/amyloplastic [Cucumis melo])

HSP 1 Score: 1193.3 bits (3086), Expect = 0.0e+00
Identity = 599/615 (97.40%), Postives = 607/615 (98.70%), Query Frame = 1

Query: 1   MASMGAVSVSNFVSRSSLISHGATASETKMSLS-VGGKNQVETHKGLKSLNNLDVLRKRT 60
           MASMGAVSVSNFVSRSSLISHGATASETK SLS VG KNQVETHKGLKSLNNLDVLRKRT
Sbjct: 1   MASMGAVSVSNFVSRSSLISHGATASETKTSLSQVGVKNQVETHKGLKSLNNLDVLRKRT 60

Query: 61  PASAICRTVSRKTDGLGGTAGKIVCEQGMNIVFVAAEVTPWSKTGGLGDVLGGLPPAMAA 120
           PA AICRT+SRKTDGLGGTAGKIVCEQGMNIVFVAAEVTPWSKTGGLGDVLGGLPPA+AA
Sbjct: 61  PAIAICRTMSRKTDGLGGTAGKIVCEQGMNIVFVAAEVTPWSKTGGLGDVLGGLPPALAA 120

Query: 121 RGHRVMTIAPRYDQYKDAWDTDVTVEIQVGDAILTVRFFHCYKRGVDRVFVDHPIFLEKV 180
           RGHRVMTIAPRYDQYKDAWDT+VTVEIQVGD ILTVRFFHCYKRGVDRVFVDHPIFLEKV
Sbjct: 121 RGHRVMTIAPRYDQYKDAWDTNVTVEIQVGDEILTVRFFHCYKRGVDRVFVDHPIFLEKV 180

Query: 181 WGKTKSKLYGPNAGVDYDDNQLRFSLLCQAALLAPLVLNLNSSKYFSGPYGEEVVFVAND 240
           WGKTKSKLYGPNAGVDYDDNQLRFSLLCQAALLAPLVLNLNSSKYFSGPYGEEVVFVAND
Sbjct: 181 WGKTKSKLYGPNAGVDYDDNQLRFSLLCQAALLAPLVLNLNSSKYFSGPYGEEVVFVAND 240

Query: 241 WHTALLPCYLKTMYQARGIYKSAKVAFCIHNIAYQGRFAFSDFSLLNLPDQFKSSFDFTD 300
           WHTALLPCYLKTMYQARGIY SAKVAFCIHNIAYQGRFAFSDF+LLNLPDQFKSSFDFTD
Sbjct: 241 WHTALLPCYLKTMYQARGIYASAKVAFCIHNIAYQGRFAFSDFALLNLPDQFKSSFDFTD 300

Query: 301 GYVKPVKGRKINWMKAGILESDRVLTVSPYYAQELVSGIERGVELDSILRLTTVTGILNG 360
           GYVKPVKGRKINWMKAGILESDRVLTVSPYYAQELVSG+ERGVELDSILRLTTVTGILNG
Sbjct: 301 GYVKPVKGRKINWMKAGILESDRVLTVSPYYAQELVSGVERGVELDSILRLTTVTGILNG 360

Query: 361 MDVQEWNPSTDKYIPIKFNAETVAEAKALLKEALQAEVGLPVDRNIPLIGFIGRLEEQKG 420
           MDVQEWNP+TDKYIPIKFNAETVAEAKALLKEALQAEVGLPVDRNIPLIGFIGRLEEQKG
Sbjct: 361 MDVQEWNPTTDKYIPIKFNAETVAEAKALLKEALQAEVGLPVDRNIPLIGFIGRLEEQKG 420

Query: 421 SDILSAAIPNFIHEDVQLVVLGTGKKYLEKQLEELEIKYPDKARGVAKFNVPLAHLINAA 480
           SDILSAAIP+FIHEDVQLVVLGTGKKYLEKQLEELEIKYPDKARGVAKFNVPLAHLINAA
Sbjct: 421 SDILSAAIPDFIHEDVQLVVLGTGKKYLEKQLEELEIKYPDKARGVAKFNVPLAHLINAA 480

Query: 481 ADFLMIPSRFEPCGLIQLQAMPYGTIPLVSTTGGLADTVIDGYTGLQMGAFSVECETVSP 540
           ADFL+IPSRFEPCGLIQLQAMPYGTIPLVSTTGGLADTVIDGYTGLQMGAFSVECE VSP
Sbjct: 481 ADFLIIPSRFEPCGLIQLQAMPYGTIPLVSTTGGLADTVIDGYTGLQMGAFSVECEMVSP 540

Query: 541 ADAAAIAKTVLRAVALFGTAAHKEMIQNCMAQDFSWKGPAKQWEKFLLGLGVENSQAGIE 600
           ADAAAIAKTVLRAVALFGTAAHKEMIQNCMAQDFSWKGPAKQWEKFLLGLGVENSQ GIE
Sbjct: 541 ADAAAIAKTVLRAVALFGTAAHKEMIQNCMAQDFSWKGPAKQWEKFLLGLGVENSQPGIE 600

Query: 601 GEEIAPLAKENVATP 615
           GEEIAPLAKENVATP
Sbjct: 601 GEEIAPLAKENVATP 615

BLAST of Cucsa.193420 vs. NCBI nr
Match: gi|343410565|gb|AEM24398.1| (granule-bound starch synthase [Cucurbita moschata])

HSP 1 Score: 1103.6 bits (2853), Expect = 0.0e+00
Identity = 556/614 (90.55%), Postives = 581/614 (94.63%), Query Frame = 1

Query: 3   SMGAVSVSNFVSRSSLI-SHGATASETKMSLS-VGGKNQVETHKGLKSLNNLDVLRKRTP 62
           SMGAV+VSN VSRSSLI SHG + SETK SLS VG KNQVETHKGLKSLNNLD+LRK+TP
Sbjct: 8   SMGAVTVSNSVSRSSLILSHGHSVSETKKSLSPVGLKNQVETHKGLKSLNNLDMLRKKTP 67

Query: 63  ASAICRTVSRKTDGLGGTAGKIVCEQGMNIVFVAAEVTPWSKTGGLGDVLGGLPPAMAAR 122
           +SAICRT SRKTDG   TAGKIVCEQGMNIV VAAEVTPWSKTGGLGDVLGGLPPA+AAR
Sbjct: 68  SSAICRTASRKTDGNCRTAGKIVCEQGMNIVLVAAEVTPWSKTGGLGDVLGGLPPALAAR 127

Query: 123 GHRVMTIAPRYDQYKDAWDTDVTVEIQVGDAILTVRFFHCYKRGVDRVFVDHPIFLEKVW 182
           GHRVMTIAPRYDQYKDAWDT+VTVE+QVGD  L VRFFHCYKRGVDRVFVDHP+FLEKVW
Sbjct: 128 GHRVMTIAPRYDQYKDAWDTNVTVEMQVGDQTLNVRFFHCYKRGVDRVFVDHPMFLEKVW 187

Query: 183 GKTKSKLYGPNAGVDYDDNQLRFSLLCQAALLAPLVLNLNSSKYFSGPYGEEVVFVANDW 242
           GKTKSKLYGP AGVDY+DNQLRFSLLCQAALLAPLVLNLNSS+YFSGPYGE+VVFVANDW
Sbjct: 188 GKTKSKLYGPKAGVDYEDNQLRFSLLCQAALLAPLVLNLNSSEYFSGPYGEDVVFVANDW 247

Query: 243 HTALLPCYLKTMYQARGIYKSAKVAFCIHNIAYQGRFAFSDFSLLNLPDQFKSSFDFTDG 302
           HTALLPCYLK+MYQ RGIY SAKVAFCIHNIAYQGRF+FSDFS LNLPDQFKSSFDFTDG
Sbjct: 248 HTALLPCYLKSMYQTRGIYSSAKVAFCIHNIAYQGRFSFSDFSFLNLPDQFKSSFDFTDG 307

Query: 303 YVKPVKGRKINWMKAGILESDRVLTVSPYYAQELVSGIERGVELDSILRLTTVTGILNGM 362
           Y KPVKGRKIN MKAGILE+DRVLTVSPYYAQELVSG+ERGVELD+ LRLTTVTGILNGM
Sbjct: 308 YEKPVKGRKINRMKAGILEADRVLTVSPYYAQELVSGVERGVELDNSLRLTTVTGILNGM 367

Query: 363 DVQEWNPSTDKYIPIKFNAETVAEAKALLKEALQAEVGLPVDRNIPLIGFIGRLEEQKGS 422
           DVQEWNP+TDKYIPIKFNAE V EAKALLKEALQAEVGLPVDRNIPLIGFIGRLEEQKGS
Sbjct: 368 DVQEWNPATDKYIPIKFNAEAVTEAKALLKEALQAEVGLPVDRNIPLIGFIGRLEEQKGS 427

Query: 423 DILSAAIPNFIHEDVQLVVLGTGKKYLEKQLEELEIKYPDKARGVAKFNVPLAHLINAAA 482
           DILSAAIP FI E+VQLVVLGTGKKYLEKQLEELEIKYPDKARGVAKFNVPLAHLINAAA
Sbjct: 428 DILSAAIPTFIDENVQLVVLGTGKKYLEKQLEELEIKYPDKARGVAKFNVPLAHLINAAA 487

Query: 483 DFLMIPSRFEPCGLIQLQAMPYGTIPLVSTTGGLADTVIDGYTGLQMGAFSVECETVSPA 542
           DFL+IPSRFEPCGLIQLQAMPYGTIPLVSTTGGLADTVIDGYTGLQMGAFSVECETV PA
Sbjct: 488 DFLIIPSRFEPCGLIQLQAMPYGTIPLVSTTGGLADTVIDGYTGLQMGAFSVECETVDPA 547

Query: 543 DAAAIAKTVLRAVALFGTAAHKEMIQNCMAQDFSWKGPAKQWEKFLLGLGVENSQAGIEG 602
           DAAAIAKTVLRA+AL+G+AA +EM QNCMAQDFSWKGPAK WEK LL LGV+NSQ GIEG
Sbjct: 548 DAAAIAKTVLRALALYGSAALEEMRQNCMAQDFSWKGPAKLWEKLLLSLGVDNSQPGIEG 607

Query: 603 EEIAPLAKENVATP 615
           EEIAPLAKENVATP
Sbjct: 608 EEIAPLAKENVATP 621

BLAST of Cucsa.193420 vs. NCBI nr
Match: gi|1009170436|ref|XP_015866198.1| (PREDICTED: granule-bound starch synthase 1, chloroplastic/amyloplastic-like [Ziziphus jujuba])

HSP 1 Score: 947.2 bits (2447), Expect = 1.4e-272
Identity = 464/615 (75.45%), Postives = 530/615 (86.18%), Query Frame = 1

Query: 4   MGAVSVSNFVSRSSLIS-HGATASETKMSLS-VGGKNQVETHKGLKSLNNLDVLRKRTPA 63
           M  V+ S FVSR S I+ HGA+ SETK +L+ +G +NQ  TH GL+SLNNLDVLR RT A
Sbjct: 1   MATVTASYFVSRISTINCHGASVSETKANLAQLGQRNQTITHNGLRSLNNLDVLRMRTSA 60

Query: 64  SAICRTVSRKTDGLGGTA--GKIVCEQGMNIVFVAAEVTPWSKTGGLGDVLGGLPPAMAA 123
           + + R   + T         GKIVC QGMN++FV AEV PWSKTGGLGDVLGGLPPAMAA
Sbjct: 61  NKVARQAMKNTKKTQTERPLGKIVCGQGMNLIFVGAEVAPWSKTGGLGDVLGGLPPAMAA 120

Query: 124 RGHRVMTIAPRYDQYKDAWDTDVTVEIQVGDAILTVRFFHCYKRGVDRVFVDHPIFLEKV 183
            GHRVMT++PRYDQYKDAWDT+V VEI+VGD I TVRFFHCYKRGVDRVFVDHP+FLEKV
Sbjct: 121 NGHRVMTVSPRYDQYKDAWDTNVVVEIRVGDRIETVRFFHCYKRGVDRVFVDHPVFLEKV 180

Query: 184 WGKTKSKLYGPNAGVDYDDNQLRFSLLCQAALLAPLVLNLNSSKYFSGPYGEEVVFVAND 243
           WGKT SK+YGP AG+DY DNQLRFSLLCQAAL AP VLNLN+SKY+SGPYGE+VVF+AND
Sbjct: 181 WGKTGSKIYGPVAGIDYKDNQLRFSLLCQAALEAPRVLNLNNSKYYSGPYGEDVVFIAND 240

Query: 244 WHTALLPCYLKTMYQARGIYKSAKVAFCIHNIAYQGRFAFSDFSLLNLPDQFKSSFDFTD 303
           WHTALLPCYLK+MY++RG+YKSAKV FCIHNIAYQGRFAFSDFSLLNLPD+FKSSFDF D
Sbjct: 241 WHTALLPCYLKSMYKSRGLYKSAKVVFCIHNIAYQGRFAFSDFSLLNLPDKFKSSFDFID 300

Query: 304 GYVKPVKGRKINWMKAGILESDRVLTVSPYYAQELVSGIERGVELDSILRLTTVTGILNG 363
           GY KP+KGRKINWMKAG+LESDR  TVSPYYAQELVSG ++GVELD+I+R T +TGI+NG
Sbjct: 301 GYEKPIKGRKINWMKAGLLESDRAPTVSPYYAQELVSGEDKGVELDNIIRKTGITGIVNG 360

Query: 364 MDVQEWNPSTDKYIPIKFNAETVAEAKALLKEALQAEVGLPVDRNIPLIGFIGRLEEQKG 423
           MDVQEWNP+ DKYI IK++A TV +AK LLKEALQAEVGLPVDRN+P+IGFIGRLEEQKG
Sbjct: 361 MDVQEWNPAADKYIDIKYDATTVEDAKPLLKEALQAEVGLPVDRNVPVIGFIGRLEEQKG 420

Query: 424 SDILSAAIPNFIHEDVQLVVLGTGKKYLEKQLEELEIKYPDKARGVAKFNVPLAHLINAA 483
           SDIL+AAI  FI E+VQ+VVLGTGKK +EKQ+E+L++KYPDKARGVAKFNVPLAH+I A 
Sbjct: 421 SDILAAAISKFIGEEVQIVVLGTGKKPMEKQIEQLDVKYPDKARGVAKFNVPLAHMIIAG 480

Query: 484 ADFLMIPSRFEPCGLIQLQAMPYGTIPLVSTTGGLADTVIDGYTGLQMGAFSVECETVSP 543
           ADF++IPSRFEPCGLIQL AM YGT+P+V++TGGL DTV +GYTG  MGAF+VEC+ + P
Sbjct: 481 ADFMLIPSRFEPCGLIQLHAMRYGTVPIVASTGGLVDTVKEGYTGFHMGAFNVECDALDP 540

Query: 544 ADAAAIAKTVLRAVALFGTAAHKEMIQNCMAQDFSWKGPAKQWEKFLLGLGVENSQAGIE 603
           AD  A+A TV RA+A +GT A KEMIQNCMAQDFSWKGPAK WEK LL L V  S+ GIE
Sbjct: 541 ADVTAVATTVNRALATYGTHALKEMIQNCMAQDFSWKGPAKLWEKMLLSLEVAGSEPGIE 600

Query: 604 GEEIAPLAKENVATP 615
           GEEIAP AKENVATP
Sbjct: 601 GEEIAPRAKENVATP 615

BLAST of Cucsa.193420 vs. NCBI nr
Match: gi|719964271|ref|XP_010252174.1| (PREDICTED: granule-bound starch synthase 1, chloroplastic/amyloplastic [Nelumbo nucifera])

HSP 1 Score: 925.6 bits (2391), Expect = 4.4e-266
Identity = 459/621 (73.91%), Postives = 525/621 (84.54%), Query Frame = 1

Query: 4   MGAVSVSNFVSRSSLISHGA-TASETKMSLS-VGGKNQVETHKGLKSLNNLDVLRKRTPA 63
           M  V+ S FVS SSL+S+G  + S+ K +L+ +G +NQ  TH GL+S+NN+D L  R  A
Sbjct: 1   MATVTASKFVSTSSLVSYGGMSGSQPKTALAQIGLRNQTMTHHGLRSVNNVDKLNLRANA 60

Query: 64  SAI--------CRTVSRKTDGLGGTAGKIVCEQGMNIVFVAAEVTPWSKTGGLGDVLGGL 123
                      C+T + K   +      I C  GMN+VFV AEV PWSKTGGLGDVLGGL
Sbjct: 61  KTTTGQAKGKECKTRNAKERSV------IRCGSGMNLVFVGAEVGPWSKTGGLGDVLGGL 120

Query: 124 PPAMAARGHRVMTIAPRYDQYKDAWDTDVTVEIQVGDAILTVRFFHCYKRGVDRVFVDHP 183
           PPAMAA GHRVMT+APRYDQYKDAWDT V VEI+VGD I TVRFFHCYKRGVDRVFVDHP
Sbjct: 121 PPAMAANGHRVMTVAPRYDQYKDAWDTSVLVEIKVGDRIETVRFFHCYKRGVDRVFVDHP 180

Query: 184 IFLEKVWGKTKSKLYGPNAGVDYDDNQLRFSLLCQAALLAPLVLNLNSSKYFSGPYGEEV 243
           +FLEKVWGKT SK+YGP AG DY DNQLRFSLLCQAAL AP VLNLNSSK+FSGPYGE+V
Sbjct: 181 MFLEKVWGKTGSKIYGPMAGEDYSDNQLRFSLLCQAALEAPRVLNLNSSKHFSGPYGEDV 240

Query: 244 VFVANDWHTALLPCYLKTMYQARGIYKSAKVAFCIHNIAYQGRFAFSDFSLLNLPDQFKS 303
           VF+ NDWHTALLPCYLKTMY++RGIY++AKVAFCIHNIAYQGRF+FSDFSLLNLPD+FKS
Sbjct: 241 VFICNDWHTALLPCYLKTMYKSRGIYRNAKVAFCIHNIAYQGRFSFSDFSLLNLPDEFKS 300

Query: 304 SFDFTDGYVKPVKGRKINWMKAGILESDRVLTVSPYYAQELVSGIERGVELDSILRLTTV 363
           SFDF DGY KPVKGRKINWMKAGILESDRVLTVSPYYA+ELVSGIE+GVELD+I+R T +
Sbjct: 301 SFDFIDGYDKPVKGRKINWMKAGILESDRVLTVSPYYAEELVSGIEKGVELDNIIRKTGI 360

Query: 364 TGILNGMDVQEWNPSTDKYIPIKFNAETVAEAKALLKEALQAEVGLPVDRNIPLIGFIGR 423
           TGI+NG DVQEWNP+TDKYI +K++A TV +AK LLKEALQ+EVGLPVDRNIP+IGFIGR
Sbjct: 361 TGIVNGTDVQEWNPTTDKYISVKYDATTVMDAKPLLKEALQSEVGLPVDRNIPVIGFIGR 420

Query: 424 LEEQKGSDILSAAIPNFIHEDVQLVVLGTGKKYLEKQLEELEIKYPDKARGVAKFNVPLA 483
           LEEQKGSDIL+A+IP FI E+VQ++VLGTGKK  EKQLE+LEIKYPDKARGVAKFNVPLA
Sbjct: 421 LEEQKGSDILAASIPKFIGENVQIIVLGTGKKAFEKQLEQLEIKYPDKARGVAKFNVPLA 480

Query: 484 HLINAAADFLMIPSRFEPCGLIQLQAMPYGTIPLVSTTGGLADTVIDGYTGLQMGAFSVE 543
           H+I A ADFL+IPSRFEPCGLIQLQ MPYGTIPLVS+TGGL DTV +GYTG  MGAF+V+
Sbjct: 481 HMIIAGADFLLIPSRFEPCGLIQLQTMPYGTIPLVSSTGGLVDTVKEGYTGFHMGAFNVD 540

Query: 544 CETVSPADAAAIAKTVLRAVALFGTAAHKEMIQNCMAQDFSWKGPAKQWEKFLLGLGVEN 603
           C+ V PAD  A+A TV +A+  +GT+A  +MI+NCMAQ+FSWKGPAK+WE  LL L V  
Sbjct: 541 CDAVDPADVNAVATTVKKALKTYGTSAMIQMIKNCMAQNFSWKGPAKKWESILLSLEVAG 600

Query: 604 SQAGIEGEEIAPLAKENVATP 615
           S+ GI+GEEIAPLAKENVATP
Sbjct: 601 SEPGIDGEEIAPLAKENVATP 615