Cucsa.192720 (gene) Cucumber (Gy14) v1

BLAST of Cucsa.192720 vs. Swiss-Prot
Match: HEM3_PEA (Porphobilinogen deaminase, chloroplastic OS=Pisum sativum GN=HEMC PE=1 SV=1)

HSP 1 Score: 550.4 bits (1417), Expect = 1.5e-155
Identity = 283/349 (81.09%), Postives = 313/349 (89.68%), Query Frame = 1

Query: 30  SLSLKPPTFSNGAKK---FHGVGLIRAVAAEQQVEKTKVALLRIGTRGSPLALAQAHETR 89
           SLSL   +F   + K   F    +  ++A EQQ ++ K AL+RIGTRGSPLALAQAHETR
Sbjct: 21  SLSLFTSSFRFSSFKTSPFSKCRIRASLAVEQQTQQNKTALIRIGTRGSPLALAQAHETR 80

Query: 90  DKLMASHPELAEDGAIQIVVIKTTGDKILSQPLADIGGKGLFTKEIDDALINGDIDIAVH 149
           DKLMASH ELAE+GAIQIV+IKTTGDKILSQPLADIGGKGLFTKEID+ALINGDIDIAVH
Sbjct: 81  DKLMASHTELAEEGAIQIVIIKTTGDKILSQPLADIGGKGLFTKEIDEALINGDIDIAVH 140

Query: 150 SMKDVPTYLPEKTILPCNLPREDVRDAFISLSAGSFAELPAGSIIGTASLRRKSQLLNRY 209
           SMKDVPTYLPE+TILPCNLPREDVRDAFISLSA S A+LPAGS+IGTASLRRKSQ+L+RY
Sbjct: 141 SMKDVPTYLPEETILPCNLPREDVRDAFISLSAASLADLPAGSVIGTASLRRKSQILHRY 200

Query: 210 PSLKVLENFRGNVQTRLRKLNEGVVQATLLALAGLRRLNMTENVTSILSIDEMLPAVAQG 269
           PSL V +NFRGNVQTRLRKL+EGVV+ATLLALAGL+RLNMTENVTS LSID+MLPAVAQG
Sbjct: 201 PSLTVQDNFRGNVQTRLRKLSEGVVKATLLALAGLKRLNMTENVTSTLSIDDMLPAVAQG 260

Query: 270 AIGIACRSDDDIMVNYLASLNHEETRLAVVCERAFLETLDGSCRTPIAGYASRDEDGNCI 329
           AIGIACRS+DD M  YLASLNHEETRLA+ CERAFL TLDGSCRTPIAGYASRD+DGNC+
Sbjct: 261 AIGIACRSNDDKMAEYLASLNHEETRLAISCERAFLTTLDGSCRTPIAGYASRDKDGNCL 320

Query: 330 FKGLVASPDGTRVLETSRRGPYAIEDMIAMGKDAGQELLSRAGPGFFDS 376
           F+GLVASPDGTRVLETSR G Y  EDM+ +GKDAG+ELLSRAGPGFF+S
Sbjct: 321 FRGLVASPDGTRVLETSRIGSYTYEDMMKIGKDAGEELLSRAGPGFFNS 369

BLAST of Cucsa.192720 vs. Swiss-Prot
Match: HEM3_ARATH (Porphobilinogen deaminase, chloroplastic OS=Arabidopsis thaliana GN=HEMC PE=1 SV=1)

HSP 1 Score: 546.6 bits (1407), Expect = 2.1e-154
Identity = 274/356 (76.97%), Postives = 309/356 (86.80%), Query Frame = 1

Query: 18  CNVGSLSLLGFSSLSLKPPTFSNGAKKFHGVGLIRAVAAEQQVEKTKVALLRIGTRGSPL 77
           C++GS+S +GFS   +  P      +K    G ++A  A +Q  KT+ A++RIGTRGSPL
Sbjct: 27  CSLGSVSAIGFSLPQISSPALGKCRRKQSSSGFVKACVAVEQ--KTRTAIIRIGTRGSPL 86

Query: 78  ALAQAHETRDKLMASHPELAEDGAIQIVVIKTTGDKILSQPLADIGGKGLFTKEIDDALI 137
           ALAQA+ETR+KL   HPEL EDGAI I +IKTTGDKILSQPLADIGGKGLFTKEID+ALI
Sbjct: 87  ALAQAYETREKLKKKHPELVEDGAIHIEIIKTTGDKILSQPLADIGGKGLFTKEIDEALI 146

Query: 138 NGDIDIAVHSMKDVPTYLPEKTILPCNLPREDVRDAFISLSAGSFAELPAGSIIGTASLR 197
           NG IDIAVHSMKDVPTYLPEKTILPCNLPREDVRDAFI L+A + AELPAGS++GTASLR
Sbjct: 147 NGHIDIAVHSMKDVPTYLPEKTILPCNLPREDVRDAFICLTAATLAELPAGSVVGTASLR 206

Query: 198 RKSQLLNRYPSLKVLENFRGNVQTRLRKLNEGVVQATLLALAGLRRLNMTENVTSILSID 257
           RKSQ+L++YP+L V ENFRGNVQTRL KL  G VQATLLALAGL+RL+MTENV SILS+D
Sbjct: 207 RKSQILHKYPALHVEENFRGNVQTRLSKLQGGKVQATLLALAGLKRLSMTENVASILSLD 266

Query: 258 EMLPAVAQGAIGIACRSDDDIMVNYLASLNHEETRLAVVCERAFLETLDGSCRTPIAGYA 317
           EMLPAVAQGAIGIACR+DDD M  YLASLNHEETRLA+ CERAFLETLDGSCRTPIAGYA
Sbjct: 267 EMLPAVAQGAIGIACRTDDDKMATYLASLNHEETRLAISCERAFLETLDGSCRTPIAGYA 326

Query: 318 SRDEDGNCIFKGLVASPDGTRVLETSRRGPYAIEDMIAMGKDAGQELLSRAGPGFF 374
           S+DE+GNCIF+GLVASPDGT+VLETSR+GPY  EDM+ MGKDAGQELLSRAGPGFF
Sbjct: 327 SKDEEGNCIFRGLVASPDGTKVLETSRKGPYVYEDMVKMGKDAGQELLSRAGPGFF 380

BLAST of Cucsa.192720 vs. Swiss-Prot
Match: HEM3_ORYSJ (Porphobilinogen deaminase, chloroplastic OS=Oryza sativa subsp. japonica GN=HEMC PE=2 SV=1)

HSP 1 Score: 498.8 bits (1283), Expect = 5.1e-140
Identity = 249/325 (76.62%), Postives = 283/325 (87.08%), Query Frame = 1

Query: 51  IRAVAAEQQVEKTKVALLRIGTRGSPLALAQAHETRDKLMASHPELAEDGAIQIVVIKTT 110
           +RA  A Q   + KV+L+RIGTRGSPLALAQAHETRDKL A+H ELAE+GA++IV+IKTT
Sbjct: 33  VRAAVAVQAEAQAKVSLIRIGTRGSPLALAQAHETRDKLKAAHSELAEEGAVEIVIIKTT 92

Query: 111 GDKILSQPLADIGGKGLFTKEIDDALINGDIDIAVHSMKDVPTYLPEKTILPCNLPREDV 170
           GD IL +PLADIGGKGLFTKEIDDAL+ G IDIAVHSMKDVPTYLPE TILPCNLPREDV
Sbjct: 93  GDMILDKPLADIGGKGLFTKEIDDALLQGRIDIAVHSMKDVPTYLPEGTILPCNLPREDV 152

Query: 171 RDAFISLSAGSFAELPAGSIIGTASLRRKSQLLNRYPSLKVLENFRGNVQTRLRKLNEGV 230
           RDAFI L+A S AELPAGS++G+ASLRR+SQ+L +YPSLKV+ NFRGNVQTRLRKL EG 
Sbjct: 153 RDAFICLTASSLAELPAGSVVGSASLRRQSQILYKYPSLKVV-NFRGNVQTRLRKLKEGD 212

Query: 231 VQATLLALAGLRRLNMTENVTSILSIDEMLPAVAQGAIGIACRSDDDIMVNYLASLNHEE 290
           V ATLLALAGL+RLNM E  TS+LS+DEMLPAVAQGAIGIACRS DD M+NYL+SLNHE+
Sbjct: 213 VHATLLALAGLKRLNMAETATSVLSVDEMLPAVAQGAIGIACRSSDDTMMNYLSSLNHED 272

Query: 291 TRLAVVCERAFLETLDGSCRTPIAGYASRDEDGNCIFKGLVASPDGTRVLETSRRGPYAI 350
           TRLAV CER FL  LDG+CRTPIA YASRD+DGNC F+GL+ASPDG+ V ETSR GPY  
Sbjct: 273 TRLAVACEREFLSVLDGNCRTPIAAYASRDKDGNCSFRGLLASPDGSTVYETSRTGPYDF 332

Query: 351 EDMIAMGKDAGQELLSRAGPGFFDS 376
           + M+ MGKDAG EL ++AGPGFFDS
Sbjct: 333 DIMVEMGKDAGHELKAKAGPGFFDS 356

BLAST of Cucsa.192720 vs. Swiss-Prot
Match: HEM3_PARDP (Porphobilinogen deaminase OS=Paracoccus denitrificans (strain Pd 1222) GN=hemC PE=3 SV=1)

HSP 1 Score: 315.1 bits (806), Expect = 1.1e-84
Identity = 171/307 (55.70%), Postives = 217/307 (70.68%), Query Frame = 1

Query: 68  LRIGTRGSPLALAQAHETRDKLMASHPELAEDGAIQIVVIKTTGDKILSQPLADIGGKGL 127
           +RIGTRGS LALAQAHETRD+LMA+H   A+  A +IVVIKTTGD++L +PL +IGGKGL
Sbjct: 11  IRIGTRGSALALAQAHETRDRLMAAHGLAAD--AFRIVVIKTTGDRVLDRPLKEIGGKGL 70

Query: 128 FTKEIDDALINGDIDIAVHSMKDVPTYLPEKTILPCNLPREDVRDAFISLSAGSFAELPA 187
           FT+EI+DAL+  +IDIAVHSMKD+PT  PE  ++ C LPREDVRDAF+S    + +ELP 
Sbjct: 71  FTREIEDALLAHEIDIAVHSMKDMPTIQPEGLVIDCYLPREDVRDAFVSAQFAAISELPQ 130

Query: 188 GSIIGTASLRRKSQLLNRYPSLKVLENFRGNVQTRLRKLNEGVVQATLLALAGLRRLNMT 247
           G+++G++SLRR++QL  R P LK++E FRGNVQTRL+KL +GV  AT LA+AGL RL M 
Sbjct: 131 GAVVGSSSLRRRAQLAARRPDLKLVE-FRGNVQTRLKKLEDGVAVATFLAMAGLTRLGML 190

Query: 248 ENVTSILSIDEMLPAVAQGAIGIACRSDDDIMVNYLASLNHEETRLAVVCERAFLETLDG 307
                 +  DEMLPAVAQG IG+  R+DD    + LA+++  ++ L V  ERAFL  LDG
Sbjct: 191 HVARGAVEPDEMLPAVAQGCIGVERRADDARTASLLAAISDRDSALRVTAERAFLARLDG 250

Query: 308 SCRTPIAGYASRDEDGNCIFKGLVASPDGTRVLETSRRGPYAIEDMIAMGKDAGQELLSR 367
           SC+TPIAG A    D     +G +  PDG+ V+   R GP A  D  AMG D  +EL  R
Sbjct: 251 SCQTPIAGLAELQGD-RLRLRGEILRPDGSEVIAAERVGPAA--DGAAMGTDLAEELRGR 310

Query: 368 AGPGFFD 375
           A   FFD
Sbjct: 311 APADFFD 311

BLAST of Cucsa.192720 vs. Swiss-Prot
Match: HEM3_NITWN (Porphobilinogen deaminase OS=Nitrobacter winogradskyi (strain Nb-255 / ATCC 25391) GN=hemC PE=3 SV=1)

HSP 1 Score: 310.5 bits (794), Expect = 2.6e-83
Identity = 174/316 (55.06%), Postives = 214/316 (67.72%), Query Frame = 1

Query: 58  QQVEKTKVALLRIGTRGSPLALAQAHETRDKLMASHPELAEDGAIQIVVIKTTGDKILSQ 117
           Q  ++T + L  IGTRGSPLALAQAHE RD+L  +H    E   I I  I+T+GD I  +
Sbjct: 2   QSSDETDI-LATIGTRGSPLALAQAHEVRDRLARAHQVAPE--RIAIKTIRTSGDAIQDR 61

Query: 118 PLADIGGKGLFTKEIDDALINGDIDIAVHSMKDVPTYLPEKTILPCNLPREDVRDAFISL 177
           PL D+GGKGLFTKEI++AL+ G ID AVHS KDVPT+LP+ T LP  LPREDVRD FIS 
Sbjct: 62  PLFDVGGKGLFTKEIEEALLAGTIDFAVHSSKDVPTFLPDATWLPAFLPREDVRDVFISP 121

Query: 178 SAGSFAELPAGSIIGTASLRRKSQLLNRYPSLKVLENFRGNVQTRLRKLNEGVVQATLLA 237
            AGS  +LPAG+ +GTASLRR++ +L   P LKV  + RGNV+TRLRK++ G   ATLLA
Sbjct: 122 HAGSLNDLPAGATVGTASLRRQAMVLKLRPDLKV-NSLRGNVETRLRKISVGEADATLLA 181

Query: 238 LAGLRRLNMTENVTSILSIDEMLPAVAQGAIGIACRSDDDIMVNYLASLNHEETRLAVVC 297
           LAGL RL + +  T IL  DE LPAV QGAI I  R DDD +  ++ ++   ET +A+  
Sbjct: 182 LAGLNRLGLQDKATRILETDEFLPAVGQGAIAIESRRDDDRINAFVKAIGDPETEVALSA 241

Query: 298 ERAFLETLDGSCRTPIAGYASRDEDGNCIFKGLVASPDGTRVLETSRRGPYAIEDMIAMG 357
           ER+FL  LDGSCRTPI G+   + D    F+GL+ SPDGT   ET+R G  A  D  A+G
Sbjct: 242 ERSFLALLDGSCRTPIGGHCRVNGD-RIDFRGLIISPDGTEFYETTREGARA--DAAALG 301

Query: 358 KDAGQELLSRAGPGFF 374
            DA  EL  RAG  FF
Sbjct: 302 ADAAHELRERAGEKFF 310

BLAST of Cucsa.192720 vs. TrEMBL
Match: A0A0A0L9F2_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_3G682170 PE=3 SV=1)

HSP 1 Score: 642.1 bits (1655), Expect = 4.2e-181
Identity = 330/331 (99.70%), Postives = 330/331 (99.70%), Query Frame = 1

Query: 45  FHGVGLIRAVAAEQQVEKTKVALLRIGTRGSPLALAQAHETRDKLMASHPELAEDGAIQI 104
           FHGVGLIRAVAAEQQVEKTKVALLRIGTRGSPLALAQAHETRDKLMASHPELAEDGAIQI
Sbjct: 34  FHGVGLIRAVAAEQQVEKTKVALLRIGTRGSPLALAQAHETRDKLMASHPELAEDGAIQI 93

Query: 105 VVIKTTGDKILSQPLADIGGKGLFTKEIDDALINGDIDIAVHSMKDVPTYLPEKTILPCN 164
           VVIKTTGDKILSQPLADIGGKGLFTKEIDDALINGDIDIAVHSMKDVPTYLPEKTILPCN
Sbjct: 94  VVIKTTGDKILSQPLADIGGKGLFTKEIDDALINGDIDIAVHSMKDVPTYLPEKTILPCN 153

Query: 165 LPREDVRDAFISLSAGSFAELPAGSIIGTASLRRKSQLLNRYPSLKVLENFRGNVQTRLR 224
           LPREDVRDAFISLSAGSFAELPAGSIIGTASLRRKSQLLNRYPSLKVLENFRGNVQTRLR
Sbjct: 154 LPREDVRDAFISLSAGSFAELPAGSIIGTASLRRKSQLLNRYPSLKVLENFRGNVQTRLR 213

Query: 225 KLNEGVVQATLLALAGLRRLNMTENVTSILSIDEMLPAVAQGAIGIACRSDDDIMVNYLA 284
           KLNEGVVQATLLALAGLRRLNMTENVTSILSIDEMLPAVAQGAIGIACRSDDDIM NYLA
Sbjct: 214 KLNEGVVQATLLALAGLRRLNMTENVTSILSIDEMLPAVAQGAIGIACRSDDDIMANYLA 273

Query: 285 SLNHEETRLAVVCERAFLETLDGSCRTPIAGYASRDEDGNCIFKGLVASPDGTRVLETSR 344
           SLNHEETRLAVVCERAFLETLDGSCRTPIAGYASRDEDGNCIFKGLVASPDGTRVLETSR
Sbjct: 274 SLNHEETRLAVVCERAFLETLDGSCRTPIAGYASRDEDGNCIFKGLVASPDGTRVLETSR 333

Query: 345 RGPYAIEDMIAMGKDAGQELLSRAGPGFFDS 376
           RGPYAIEDMIAMGKDAGQELLSRAGPGFFDS
Sbjct: 334 RGPYAIEDMIAMGKDAGQELLSRAGPGFFDS 364

BLAST of Cucsa.192720 vs. TrEMBL
Match: W9SZ70_9ROSA (Porphobilinogen deaminase OS=Morus notabilis GN=L484_007545 PE=3 SV=1)

HSP 1 Score: 593.2 bits (1528), Expect = 2.2e-166
Identity = 307/369 (83.20%), Postives = 333/369 (90.24%), Query Frame = 1

Query: 14  MPRPC------NVGSLSLLGFSSLSLKPPTFSNGAKKFHGVGLIRA-VAAEQQVEKTKVA 73
           M RPC      + GS+S+LGFS  SLK  +     ++ HG+G+ RA VA EQQ +K++VA
Sbjct: 17  MARPCFPVSFSSSGSVSVLGFSLPSLKTTS-----RRKHGIGVTRASVAVEQQTQKSRVA 76

Query: 74  LLRIGTRGSPLALAQAHETRDKLMASHPELAEDGAIQIVVIKTTGDKILSQPLADIGGKG 133
           LLRIGTRGSPLALAQAHETRDKL ASHPELAE+GAI+IV+IKTTGDKILSQPLADIGGKG
Sbjct: 77  LLRIGTRGSPLALAQAHETRDKLKASHPELAEEGAIEIVIIKTTGDKILSQPLADIGGKG 136

Query: 134 LFTKEIDDALINGDIDIAVHSMKDVPTYLPEKTILPCNLPREDVRDAFISLSAGSFAELP 193
           LFTKEID+ALIN DIDIAVHSMKDVPTYLPEKTILPCNLPREDVRDAFISLSA S AELP
Sbjct: 137 LFTKEIDEALINSDIDIAVHSMKDVPTYLPEKTILPCNLPREDVRDAFISLSAASLAELP 196

Query: 194 AGSIIGTASLRRKSQLLNRYPSLKVLENFRGNVQTRLRKLNEGVVQATLLALAGLRRLNM 253
           AGSI+GTASLRRKSQ+L RYPSLKV +NFRGNVQTRLRKLNEGVVQATLLALAGL+RLNM
Sbjct: 197 AGSIVGTASLRRKSQILYRYPSLKVEDNFRGNVQTRLRKLNEGVVQATLLALAGLKRLNM 256

Query: 254 TENVTSILSIDEMLPAVAQGAIGIACRSDDDIMVNYLASLNHEETRLAVVCERAFLETLD 313
           TENVT ILSIDEMLPAVAQGAIGIACRSDDD M +Y+ASLNHEETRLA+ CERAFL  LD
Sbjct: 257 TENVTCILSIDEMLPAVAQGAIGIACRSDDDKMASYIASLNHEETRLAIACERAFLTKLD 316

Query: 314 GSCRTPIAGYASRDEDGNCIFKGLVASPDGTRVLETSRRGPYAIEDMIAMGKDAGQELLS 373
           GSCRTPIAGYAS+DEDGNCIFKGLVASPDGTRVLETSR+GPYA EDM+ MGKDAG+ELLS
Sbjct: 317 GSCRTPIAGYASKDEDGNCIFKGLVASPDGTRVLETSRKGPYAFEDMMKMGKDAGEELLS 376

Query: 374 RAGPGFFDS 376
           RAGPGFFDS
Sbjct: 377 RAGPGFFDS 380

BLAST of Cucsa.192720 vs. TrEMBL
Match: M5XYM7_PRUPE (Uncharacterized protein OS=Prunus persica GN=PRUPE_ppa007124mg PE=3 SV=1)

HSP 1 Score: 587.0 bits (1512), Expect = 1.6e-164
Identity = 302/356 (84.83%), Postives = 327/356 (91.85%), Query Frame = 1

Query: 21  GSLSLLGFSSLSLKPPTFSNGAKKFHGVGLIRA-VAAEQQVEKTKVALLRIGTRGSPLAL 80
           GS+S+ GFS  SLK   F +  +K   VG+ RA VA EQQ +K K+AL+RIGTRGSPLAL
Sbjct: 26  GSVSVPGFSLPSLKTRAFPHCIRKHSAVGIPRASVAVEQQTQKAKLALIRIGTRGSPLAL 85

Query: 81  AQAHETRDKLMASHPELAEDGAIQIVVIKTTGDKILSQPLADIGGKGLFTKEIDDALING 140
           AQAHETRDKLMASHP+LAE+GAIQIV+IKTTGDKILSQPLADIGGKGLFTKEID+ALING
Sbjct: 86  AQAHETRDKLMASHPDLAEEGAIQIVIIKTTGDKILSQPLADIGGKGLFTKEIDEALING 145

Query: 141 DIDIAVHSMKDVPTYLPEKTILPCNLPREDVRDAFISLSAGSFAELPAGSIIGTASLRRK 200
           +IDIAVHSMKDVPTYLPEKTILPCNLPREDVRDAFISL+A S A+LPAGS IGTASLRRK
Sbjct: 146 EIDIAVHSMKDVPTYLPEKTILPCNLPREDVRDAFISLTASSLADLPAGSTIGTASLRRK 205

Query: 201 SQLLNRYPSLKVLENFRGNVQTRLRKLNEGVVQATLLALAGLRRLNMTENVTSILSIDEM 260
           SQ+LNRYPSL VLENFRGNVQTRLRKLNE VVQATLLALAGL+RL+MTENVTSILS+DEM
Sbjct: 206 SQILNRYPSLNVLENFRGNVQTRLRKLNEKVVQATLLALAGLKRLDMTENVTSILSLDEM 265

Query: 261 LPAVAQGAIGIACRSDDDIMVNYLASLNHEETRLAVVCERAFLETLDGSCRTPIAGYASR 320
           LPAVAQGAIGIACRS+DD M NY+ASLNHEETRLAV CERAFL TLDGSCRTPIAGYASR
Sbjct: 266 LPAVAQGAIGIACRSNDDKMANYIASLNHEETRLAVACERAFLLTLDGSCRTPIAGYASR 325

Query: 321 DEDGNCIFKGLVASPDGTRVLETSRRGPYAIEDMIAMGKDAGQELLSRAGPGFFDS 376
           DEDGNCIFKGLVASPDGTRVLETSR+G YA +DMI MGK+AGQELLS+AGPGFFDS
Sbjct: 326 DEDGNCIFKGLVASPDGTRVLETSRKGTYAFQDMINMGKEAGQELLSQAGPGFFDS 381

BLAST of Cucsa.192720 vs. TrEMBL
Match: A0A0D2RJ21_GOSRA (Uncharacterized protein OS=Gossypium raimondii GN=B456_005G211000 PE=3 SV=1)

HSP 1 Score: 583.2 bits (1502), Expect = 2.3e-163
Identity = 302/357 (84.59%), Postives = 324/357 (90.76%), Query Frame = 1

Query: 18  CNVGSLSLLGFSSLSLKPPTFSNGAKKFHGVGLIRAVAAEQQVEKTKVALLRIGTRGSPL 77
           C  GS+S+LGF    LK  +  +  KK  G G+I+A      V KT+VAL+RIGTRGSPL
Sbjct: 25  CGGGSVSVLGFPLQHLKIQSLPDSKKK-QGFGVIKA-----SVAKTEVALVRIGTRGSPL 84

Query: 78  ALAQAHETRDKLMASHPELAEDGAIQIVVIKTTGDKILSQPLADIGGKGLFTKEIDDALI 137
           ALAQAHETRDKLMASHPELAE+GAI+IVVIKTTGDKILSQPLADIGGKGLFTKEID+ALI
Sbjct: 85  ALAQAHETRDKLMASHPELAEEGAIKIVVIKTTGDKILSQPLADIGGKGLFTKEIDEALI 144

Query: 138 NGDIDIAVHSMKDVPTYLPEKTILPCNLPREDVRDAFISLSAGSFAELPAGSIIGTASLR 197
           NGDIDIAVHSMKDVPTYLPEKTILPCNLPREDVRDAFISLSA S AELPAGS+IGTASLR
Sbjct: 145 NGDIDIAVHSMKDVPTYLPEKTILPCNLPREDVRDAFISLSASSLAELPAGSVIGTASLR 204

Query: 198 RKSQLLNRYPSLKVLENFRGNVQTRLRKLNEGVVQATLLALAGLRRLNMTENVTSILSID 257
           RKSQ+L RYPSLKV +NFRGNVQTRLRKLNEGVVQATLLALAGLRRL+MTENVTS+L I 
Sbjct: 205 RKSQILYRYPSLKVEDNFRGNVQTRLRKLNEGVVQATLLALAGLRRLSMTENVTSVLPIH 264

Query: 258 EMLPAVAQGAIGIACRSDDDIMVNYLASLNHEETRLAVVCERAFLETLDGSCRTPIAGYA 317
           EMLPAVAQGAIGIACRS+D+ M NYLASLNHEETRLAV CERAFLETLDGSCRTPIAGYA
Sbjct: 265 EMLPAVAQGAIGIACRSNDEKMANYLASLNHEETRLAVTCERAFLETLDGSCRTPIAGYA 324

Query: 318 SRDEDGNCIFKGLVASPDGTRVLETSRRGPYAIEDMIAMGKDAGQELLSRAGPGFFD 375
           S+DEDGNCIFKGLVASPDGTRVLETSR+GPYA EDM+ MGKDAG+ELLSRAGPGFFD
Sbjct: 325 SKDEDGNCIFKGLVASPDGTRVLETSRKGPYAFEDMVMMGKDAGKELLSRAGPGFFD 375

BLAST of Cucsa.192720 vs. TrEMBL
Match: B9HGK9_POPTR (Uncharacterized protein OS=Populus trichocarpa GN=POPTR_0007s07680g PE=3 SV=2)

HSP 1 Score: 579.3 bits (1492), Expect = 3.3e-162
Identity = 306/381 (80.31%), Postives = 333/381 (87.40%), Query Frame = 1

Query: 1   MGVLSSPSVSHSPMPRP------CNVGSLSLLGFSSLSLKPPTFSNGAKKFHGVGLIRA- 60
           M  LSS   S + M RP      C  GS+S  GFS   LK   FS   KK   +  ++A 
Sbjct: 1   METLSSLCTSQALMSRPSSPAIFCTSGSVSFTGFS---LKTQAFS---KKKQTLSFVKAS 60

Query: 61  VAAEQQVEKTKVALLRIGTRGSPLALAQAHETRDKLMASHPELAEDGAIQIVVIKTTGDK 120
           VA EQQ ++ KVAL+RIGTRGSPLALAQAHETRDKLMASH +LAE+GAIQIV+IKTTGDK
Sbjct: 61  VAVEQQTQEAKVALIRIGTRGSPLALAQAHETRDKLMASHSDLAEEGAIQIVIIKTTGDK 120

Query: 121 ILSQPLADIGGKGLFTKEIDDALINGDIDIAVHSMKDVPTYLPEKTILPCNLPREDVRDA 180
           I SQPLADIGGKGLFTKEID+ALINGDIDIAVHSMKDVPTYLPEKTILPCNLPREDVRDA
Sbjct: 121 IQSQPLADIGGKGLFTKEIDEALINGDIDIAVHSMKDVPTYLPEKTILPCNLPREDVRDA 180

Query: 181 FISLSAGSFAELPAGSIIGTASLRRKSQLLNRYPSLKVLENFRGNVQTRLRKLNEGVVQA 240
           FISLSA S A+LPAGSIIGTASLRRKSQ+L+RYPSL V ENFRGNVQTRLRKLNEGVV+A
Sbjct: 181 FISLSAASLADLPAGSIIGTASLRRKSQILHRYPSLSVEENFRGNVQTRLRKLNEGVVKA 240

Query: 241 TLLALAGLRRLNMTENVTSILSIDEMLPAVAQGAIGIACRSDDDIMVNYLASLNHEETRL 300
           TLLALAGL+RLNMTENVTSIL +D+MLPAVAQGAIGIACRS+DD MVNYLASLNHEETRL
Sbjct: 241 TLLALAGLKRLNMTENVTSILPLDDMLPAVAQGAIGIACRSNDDKMVNYLASLNHEETRL 300

Query: 301 AVVCERAFLETLDGSCRTPIAGYASRDEDGNCIFKGLVASPDGTRVLETSRRGPYAIEDM 360
           AV CERAFLETLDGSCRTPIAGYA +DE+G+CIFKGLVASPDG RVLETSR+GPYA +DM
Sbjct: 301 AVACERAFLETLDGSCRTPIAGYARKDENGDCIFKGLVASPDGRRVLETSRKGPYAFDDM 360

Query: 361 IAMGKDAGQELLSRAGPGFFD 375
           IAMGKDAG+ELLS+AGPGFFD
Sbjct: 361 IAMGKDAGKELLSQAGPGFFD 375

BLAST of Cucsa.192720 vs. TAIR10
Match: AT5G08280.1 (AT5G08280.1 hydroxymethylbilane synthase)

HSP 1 Score: 546.6 bits (1407), Expect = 1.2e-155
Identity = 274/356 (76.97%), Postives = 309/356 (86.80%), Query Frame = 1

Query: 18  CNVGSLSLLGFSSLSLKPPTFSNGAKKFHGVGLIRAVAAEQQVEKTKVALLRIGTRGSPL 77
           C++GS+S +GFS   +  P      +K    G ++A  A +Q  KT+ A++RIGTRGSPL
Sbjct: 27  CSLGSVSAIGFSLPQISSPALGKCRRKQSSSGFVKACVAVEQ--KTRTAIIRIGTRGSPL 86

Query: 78  ALAQAHETRDKLMASHPELAEDGAIQIVVIKTTGDKILSQPLADIGGKGLFTKEIDDALI 137
           ALAQA+ETR+KL   HPEL EDGAI I +IKTTGDKILSQPLADIGGKGLFTKEID+ALI
Sbjct: 87  ALAQAYETREKLKKKHPELVEDGAIHIEIIKTTGDKILSQPLADIGGKGLFTKEIDEALI 146

Query: 138 NGDIDIAVHSMKDVPTYLPEKTILPCNLPREDVRDAFISLSAGSFAELPAGSIIGTASLR 197
           NG IDIAVHSMKDVPTYLPEKTILPCNLPREDVRDAFI L+A + AELPAGS++GTASLR
Sbjct: 147 NGHIDIAVHSMKDVPTYLPEKTILPCNLPREDVRDAFICLTAATLAELPAGSVVGTASLR 206

Query: 198 RKSQLLNRYPSLKVLENFRGNVQTRLRKLNEGVVQATLLALAGLRRLNMTENVTSILSID 257
           RKSQ+L++YP+L V ENFRGNVQTRL KL  G VQATLLALAGL+RL+MTENV SILS+D
Sbjct: 207 RKSQILHKYPALHVEENFRGNVQTRLSKLQGGKVQATLLALAGLKRLSMTENVASILSLD 266

Query: 258 EMLPAVAQGAIGIACRSDDDIMVNYLASLNHEETRLAVVCERAFLETLDGSCRTPIAGYA 317
           EMLPAVAQGAIGIACR+DDD M  YLASLNHEETRLA+ CERAFLETLDGSCRTPIAGYA
Sbjct: 267 EMLPAVAQGAIGIACRTDDDKMATYLASLNHEETRLAISCERAFLETLDGSCRTPIAGYA 326

Query: 318 SRDEDGNCIFKGLVASPDGTRVLETSRRGPYAIEDMIAMGKDAGQELLSRAGPGFF 374
           S+DE+GNCIF+GLVASPDGT+VLETSR+GPY  EDM+ MGKDAGQELLSRAGPGFF
Sbjct: 327 SKDEEGNCIFRGLVASPDGTKVLETSRKGPYVYEDMVKMGKDAGQELLSRAGPGFF 380

BLAST of Cucsa.192720 vs. NCBI nr
Match: gi|449464030|ref|XP_004149732.1| (PREDICTED: porphobilinogen deaminase, chloroplastic [Cucumis sativus])

HSP 1 Score: 727.6 bits (1877), Expect = 1.1e-206
Identity = 373/375 (99.47%), Postives = 373/375 (99.47%), Query Frame = 1

Query: 1   MGVLSSPSVSHSPMPRPCNVGSLSLLGFSSLSLKPPTFSNGAKKFHGVGLIRAVAAEQQV 60
           MGVLSSPSVSHSPMPRPCNVGSLS LGFSSLSLKPPTFSNGAKKFHGVGLIRAVAAEQQV
Sbjct: 1   MGVLSSPSVSHSPMPRPCNVGSLSFLGFSSLSLKPPTFSNGAKKFHGVGLIRAVAAEQQV 60

Query: 61  EKTKVALLRIGTRGSPLALAQAHETRDKLMASHPELAEDGAIQIVVIKTTGDKILSQPLA 120
           EKTKVALLRIGTRGSPLALAQAHETRDKLMASHPELAEDGAIQIVVIKTTGDKILSQPLA
Sbjct: 61  EKTKVALLRIGTRGSPLALAQAHETRDKLMASHPELAEDGAIQIVVIKTTGDKILSQPLA 120

Query: 121 DIGGKGLFTKEIDDALINGDIDIAVHSMKDVPTYLPEKTILPCNLPREDVRDAFISLSAG 180
           DIGGKGLFTKEIDDALINGDIDIAVHSMKDVPTYLPEKTILPCNLPREDVRDAFISLSAG
Sbjct: 121 DIGGKGLFTKEIDDALINGDIDIAVHSMKDVPTYLPEKTILPCNLPREDVRDAFISLSAG 180

Query: 181 SFAELPAGSIIGTASLRRKSQLLNRYPSLKVLENFRGNVQTRLRKLNEGVVQATLLALAG 240
           SFAELPAGSIIGTASLRRKSQLLNRYPSLKVLENFRGNVQTRLRKLNEGVVQATLLALAG
Sbjct: 181 SFAELPAGSIIGTASLRRKSQLLNRYPSLKVLENFRGNVQTRLRKLNEGVVQATLLALAG 240

Query: 241 LRRLNMTENVTSILSIDEMLPAVAQGAIGIACRSDDDIMVNYLASLNHEETRLAVVCERA 300
           LRRLNMTENVTSILSIDEMLPAVAQGAIGIACRSDDDIM NYLASLNHEETRLAVVCERA
Sbjct: 241 LRRLNMTENVTSILSIDEMLPAVAQGAIGIACRSDDDIMANYLASLNHEETRLAVVCERA 300

Query: 301 FLETLDGSCRTPIAGYASRDEDGNCIFKGLVASPDGTRVLETSRRGPYAIEDMIAMGKDA 360
           FLETLDGSCRTPIAGYASRDEDGNCIFKGLVASPDGTRVLETSRRGPYAIEDMIAMGKDA
Sbjct: 301 FLETLDGSCRTPIAGYASRDEDGNCIFKGLVASPDGTRVLETSRRGPYAIEDMIAMGKDA 360

Query: 361 GQELLSRAGPGFFDS 376
           GQELLSRAGPGFFDS
Sbjct: 361 GQELLSRAGPGFFDS 375

BLAST of Cucsa.192720 vs. NCBI nr
Match: gi|700203422|gb|KGN58555.1| (hypothetical protein Csa_3G682170 [Cucumis sativus])

HSP 1 Score: 642.1 bits (1655), Expect = 6.0e-181
Identity = 330/331 (99.70%), Postives = 330/331 (99.70%), Query Frame = 1

Query: 45  FHGVGLIRAVAAEQQVEKTKVALLRIGTRGSPLALAQAHETRDKLMASHPELAEDGAIQI 104
           FHGVGLIRAVAAEQQVEKTKVALLRIGTRGSPLALAQAHETRDKLMASHPELAEDGAIQI
Sbjct: 34  FHGVGLIRAVAAEQQVEKTKVALLRIGTRGSPLALAQAHETRDKLMASHPELAEDGAIQI 93

Query: 105 VVIKTTGDKILSQPLADIGGKGLFTKEIDDALINGDIDIAVHSMKDVPTYLPEKTILPCN 164
           VVIKTTGDKILSQPLADIGGKGLFTKEIDDALINGDIDIAVHSMKDVPTYLPEKTILPCN
Sbjct: 94  VVIKTTGDKILSQPLADIGGKGLFTKEIDDALINGDIDIAVHSMKDVPTYLPEKTILPCN 153

Query: 165 LPREDVRDAFISLSAGSFAELPAGSIIGTASLRRKSQLLNRYPSLKVLENFRGNVQTRLR 224
           LPREDVRDAFISLSAGSFAELPAGSIIGTASLRRKSQLLNRYPSLKVLENFRGNVQTRLR
Sbjct: 154 LPREDVRDAFISLSAGSFAELPAGSIIGTASLRRKSQLLNRYPSLKVLENFRGNVQTRLR 213

Query: 225 KLNEGVVQATLLALAGLRRLNMTENVTSILSIDEMLPAVAQGAIGIACRSDDDIMVNYLA 284
           KLNEGVVQATLLALAGLRRLNMTENVTSILSIDEMLPAVAQGAIGIACRSDDDIM NYLA
Sbjct: 214 KLNEGVVQATLLALAGLRRLNMTENVTSILSIDEMLPAVAQGAIGIACRSDDDIMANYLA 273

Query: 285 SLNHEETRLAVVCERAFLETLDGSCRTPIAGYASRDEDGNCIFKGLVASPDGTRVLETSR 344
           SLNHEETRLAVVCERAFLETLDGSCRTPIAGYASRDEDGNCIFKGLVASPDGTRVLETSR
Sbjct: 274 SLNHEETRLAVVCERAFLETLDGSCRTPIAGYASRDEDGNCIFKGLVASPDGTRVLETSR 333

Query: 345 RGPYAIEDMIAMGKDAGQELLSRAGPGFFDS 376
           RGPYAIEDMIAMGKDAGQELLSRAGPGFFDS
Sbjct: 334 RGPYAIEDMIAMGKDAGQELLSRAGPGFFDS 364

BLAST of Cucsa.192720 vs. NCBI nr
Match: gi|703160461|ref|XP_010112535.1| (Porphobilinogen deaminase [Morus notabilis])

HSP 1 Score: 593.2 bits (1528), Expect = 3.2e-166
Identity = 307/369 (83.20%), Postives = 333/369 (90.24%), Query Frame = 1

Query: 14  MPRPC------NVGSLSLLGFSSLSLKPPTFSNGAKKFHGVGLIRA-VAAEQQVEKTKVA 73
           M RPC      + GS+S+LGFS  SLK  +     ++ HG+G+ RA VA EQQ +K++VA
Sbjct: 17  MARPCFPVSFSSSGSVSVLGFSLPSLKTTS-----RRKHGIGVTRASVAVEQQTQKSRVA 76

Query: 74  LLRIGTRGSPLALAQAHETRDKLMASHPELAEDGAIQIVVIKTTGDKILSQPLADIGGKG 133
           LLRIGTRGSPLALAQAHETRDKL ASHPELAE+GAI+IV+IKTTGDKILSQPLADIGGKG
Sbjct: 77  LLRIGTRGSPLALAQAHETRDKLKASHPELAEEGAIEIVIIKTTGDKILSQPLADIGGKG 136

Query: 134 LFTKEIDDALINGDIDIAVHSMKDVPTYLPEKTILPCNLPREDVRDAFISLSAGSFAELP 193
           LFTKEID+ALIN DIDIAVHSMKDVPTYLPEKTILPCNLPREDVRDAFISLSA S AELP
Sbjct: 137 LFTKEIDEALINSDIDIAVHSMKDVPTYLPEKTILPCNLPREDVRDAFISLSAASLAELP 196

Query: 194 AGSIIGTASLRRKSQLLNRYPSLKVLENFRGNVQTRLRKLNEGVVQATLLALAGLRRLNM 253
           AGSI+GTASLRRKSQ+L RYPSLKV +NFRGNVQTRLRKLNEGVVQATLLALAGL+RLNM
Sbjct: 197 AGSIVGTASLRRKSQILYRYPSLKVEDNFRGNVQTRLRKLNEGVVQATLLALAGLKRLNM 256

Query: 254 TENVTSILSIDEMLPAVAQGAIGIACRSDDDIMVNYLASLNHEETRLAVVCERAFLETLD 313
           TENVT ILSIDEMLPAVAQGAIGIACRSDDD M +Y+ASLNHEETRLA+ CERAFL  LD
Sbjct: 257 TENVTCILSIDEMLPAVAQGAIGIACRSDDDKMASYIASLNHEETRLAIACERAFLTKLD 316

Query: 314 GSCRTPIAGYASRDEDGNCIFKGLVASPDGTRVLETSRRGPYAIEDMIAMGKDAGQELLS 373
           GSCRTPIAGYAS+DEDGNCIFKGLVASPDGTRVLETSR+GPYA EDM+ MGKDAG+ELLS
Sbjct: 317 GSCRTPIAGYASKDEDGNCIFKGLVASPDGTRVLETSRKGPYAFEDMMKMGKDAGEELLS 376

Query: 374 RAGPGFFDS 376
           RAGPGFFDS
Sbjct: 377 RAGPGFFDS 380

BLAST of Cucsa.192720 vs. NCBI nr
Match: gi|1009174122|ref|XP_015868182.1| (PREDICTED: porphobilinogen deaminase, chloroplastic [Ziziphus jujuba])

HSP 1 Score: 587.8 bits (1514), Expect = 1.3e-164
Identity = 307/379 (81.00%), Postives = 339/379 (89.45%), Query Frame = 1

Query: 1   MGVLSSPSVSHSPMPRPCNV---GSLSLLGFSSLSLKPPTFSNGAKKFHGVGLIRA-VAA 60
           M  LSS  + + P+ RP N    GS+S+LGFSS S K P F++  +K   +G IRA VA 
Sbjct: 1   MESLSSCMLRNQPLTRPVNFSSSGSVSVLGFSSSSPKAPFFTSYGRK-QNIGFIRASVAV 60

Query: 61  EQQVEKTKV-ALLRIGTRGSPLALAQAHETRDKLMASHPELAEDGAIQIVVIKTTGDKIL 120
           EQ  +KTKV AL++IGTRGSPLALAQA+ETR+KL+A HPELAE+GAI I +IKTTGDKIL
Sbjct: 61  EQPTQKTKVDALIKIGTRGSPLALAQAYETREKLIAIHPELAEEGAIHIEIIKTTGDKIL 120

Query: 121 SQPLADIGGKGLFTKEIDDALINGDIDIAVHSMKDVPTYLPEKTILPCNLPREDVRDAFI 180
           SQPLADIGGKGLFTKEID+ALINGDIDIAVHSMKDVPTYLP+KTILPCNL REDVRDAFI
Sbjct: 121 SQPLADIGGKGLFTKEIDEALINGDIDIAVHSMKDVPTYLPDKTILPCNLQREDVRDAFI 180

Query: 181 SLSAGSFAELPAGSIIGTASLRRKSQLLNRYPSLKVLENFRGNVQTRLRKLNEGVVQATL 240
           SLSA S A+LPAGS++GTASLRRKSQ+L+RYPSLKV ENFRGNVQTRLRKLNEGVV+ATL
Sbjct: 181 SLSAASLADLPAGSVVGTASLRRKSQILHRYPSLKVEENFRGNVQTRLRKLNEGVVKATL 240

Query: 241 LALAGLRRLNMTENVTSILSIDEMLPAVAQGAIGIACRSDDDIMVNYLASLNHEETRLAV 300
           LALAGL+RL+MTENVTS+LSIDEMLPAVAQGAIGIACRSDDD M NYLASLNHEETRLAV
Sbjct: 241 LALAGLKRLSMTENVTSVLSIDEMLPAVAQGAIGIACRSDDDKMANYLASLNHEETRLAV 300

Query: 301 VCERAFLETLDGSCRTPIAGYASRDEDGNCIFKGLVASPDGTRVLETSRRGPYAIEDMIA 360
            CERAFLETLDGSCRTPIAGYAS+DEDGNCIFKGLVASPDGTRVLETSR+GPY  EDMI 
Sbjct: 301 ACERAFLETLDGSCRTPIAGYASKDEDGNCIFKGLVASPDGTRVLETSRKGPYLFEDMIK 360

Query: 361 MGKDAGQELLSRAGPGFFD 375
           MGKDAG+ELLS+AGPGFFD
Sbjct: 361 MGKDAGKELLSQAGPGFFD 378

BLAST of Cucsa.192720 vs. NCBI nr
Match: gi|596287238|ref|XP_007225744.1| (hypothetical protein PRUPE_ppa007124mg [Prunus persica])

HSP 1 Score: 587.0 bits (1512), Expect = 2.3e-164
Identity = 302/356 (84.83%), Postives = 327/356 (91.85%), Query Frame = 1

Query: 21  GSLSLLGFSSLSLKPPTFSNGAKKFHGVGLIRA-VAAEQQVEKTKVALLRIGTRGSPLAL 80
           GS+S+ GFS  SLK   F +  +K   VG+ RA VA EQQ +K K+AL+RIGTRGSPLAL
Sbjct: 26  GSVSVPGFSLPSLKTRAFPHCIRKHSAVGIPRASVAVEQQTQKAKLALIRIGTRGSPLAL 85

Query: 81  AQAHETRDKLMASHPELAEDGAIQIVVIKTTGDKILSQPLADIGGKGLFTKEIDDALING 140
           AQAHETRDKLMASHP+LAE+GAIQIV+IKTTGDKILSQPLADIGGKGLFTKEID+ALING
Sbjct: 86  AQAHETRDKLMASHPDLAEEGAIQIVIIKTTGDKILSQPLADIGGKGLFTKEIDEALING 145

Query: 141 DIDIAVHSMKDVPTYLPEKTILPCNLPREDVRDAFISLSAGSFAELPAGSIIGTASLRRK 200
           +IDIAVHSMKDVPTYLPEKTILPCNLPREDVRDAFISL+A S A+LPAGS IGTASLRRK
Sbjct: 146 EIDIAVHSMKDVPTYLPEKTILPCNLPREDVRDAFISLTASSLADLPAGSTIGTASLRRK 205

Query: 201 SQLLNRYPSLKVLENFRGNVQTRLRKLNEGVVQATLLALAGLRRLNMTENVTSILSIDEM 260
           SQ+LNRYPSL VLENFRGNVQTRLRKLNE VVQATLLALAGL+RL+MTENVTSILS+DEM
Sbjct: 206 SQILNRYPSLNVLENFRGNVQTRLRKLNEKVVQATLLALAGLKRLDMTENVTSILSLDEM 265

Query: 261 LPAVAQGAIGIACRSDDDIMVNYLASLNHEETRLAVVCERAFLETLDGSCRTPIAGYASR 320
           LPAVAQGAIGIACRS+DD M NY+ASLNHEETRLAV CERAFL TLDGSCRTPIAGYASR
Sbjct: 266 LPAVAQGAIGIACRSNDDKMANYIASLNHEETRLAVACERAFLLTLDGSCRTPIAGYASR 325

Query: 321 DEDGNCIFKGLVASPDGTRVLETSRRGPYAIEDMIAMGKDAGQELLSRAGPGFFDS 376
           DEDGNCIFKGLVASPDGTRVLETSR+G YA +DMI MGK+AGQELLS+AGPGFFDS
Sbjct: 326 DEDGNCIFKGLVASPDGTRVLETSRKGTYAFQDMINMGKEAGQELLSQAGPGFFDS 381