Cucsa.192110 (gene) Cucumber (Gy14) v1

BLAST of Cucsa.192110 vs. Swiss-Prot
Match: SERA2_ARATH (D-3-phosphoglycerate dehydrogenase 2, chloroplastic OS=Arabidopsis thaliana GN=PGDH2 PE=1 SV=2)

HSP 1 Score: 883.6 bits (2282), Expect = 1.3e-255
Identity = 467/631 (74.01%), Postives = 528/631 (83.68%), Query Frame = 1

Query: 29  LSFSTSSVSLNPRPLHSFSSSSSSSSSSSSNRCSFIP---------------LRSVLKTL 88
           ++FS+S  S+      + +S  +S S S S+R + +P               + + LKT+
Sbjct: 1   MAFSSSCSSVK-----AVNSRWTSPSPSPSSRFAVLPAFLHRRYATSVKLTAISAALKTV 60

Query: 89  ESSAFVSQN--------SDLLDPTSPNDRSTILVAEKLGEAGLELLRSFGNVVCAYDLSP 148
           E +     N        SD  +PT P  R  ILV EKLGEAG+ LLR FG+V C+YDLSP
Sbjct: 61  EQTTLTEDNRFSTVGSDSDEYNPTLPKPR--ILVTEKLGEAGVNLLREFGDVDCSYDLSP 120

Query: 149 EELCAKISSCDALIVRSGTKVNRQVFEAAKGRLKVVGRAGVGIDNVDLQAATEFGCLVVN 208
           E+L  K++  DALIVRSGTKV R+VFEAAKGRLKVVGRAGVGIDNVDLQAATE GCLVVN
Sbjct: 121 EDLKKKVAESDALIVRSGTKVTREVFEAAKGRLKVVGRAGVGIDNVDLQAATEHGCLVVN 180

Query: 209 APTANTIAAAEHGIALLTSMARNVAQADASMKAGKWERNKYVGVSLVGKTLAVMGFGKVG 268
           APTANT+AAAEHGIALL SMARNVAQADAS+KAGKWER+KYVGVSLVGKTLAVMGFGKVG
Sbjct: 181 APTANTVAAAEHGIALLASMARNVAQADASIKAGKWERSKYVGVSLVGKTLAVMGFGKVG 240

Query: 269 SEVARRAKGLGMQVIAHDPYAPVDRARAIGVELVSFNQAISTADFISLHMPLTPTTSKVF 328
           +EVARRAKGLGM VI+HDPYAP DRARA+GV+LVSF+QAISTADF+SLHMPLTP T KVF
Sbjct: 241 TEVARRAKGLGMTVISHDPYAPADRARALGVDLVSFDQAISTADFVSLHMPLTPATKKVF 300

Query: 329 NDDTFGLMKKGARLINVARGGVIDEDALVRALDSGAVAQAALDVFVEEPPPKDSKLVQHK 388
           ND+TF  MKKG RLINVARGGVIDEDALVRALD+G VAQAALDVF EEPP KDS+L+QH+
Sbjct: 301 NDETFSKMKKGVRLINVARGGVIDEDALVRALDAGIVAQAALDVFCEEPPSKDSRLIQHE 360

Query: 389 NVTVTPHLGASTKEAQEGVAIEIAEAVVGALNGELSATAVNAPMVPPEVLSELAPYVVLA 448
           NVTVTPHLGASTKEAQEGVAIEIAEAV GAL GELSATAVNAPMV PEVLSEL PY+VLA
Sbjct: 361 NVTVTPHLGASTKEAQEGVAIEIAEAVAGALKGELSATAVNAPMVAPEVLSELTPYIVLA 420

Query: 449 EKLGRLAVQLVAGGSGIKSVKVVYRSGRAPDDLDTRLLRAMITKGIIEPISDSHINLVNA 508
           EKLGRLAVQL +GG G++S++VVYRS R  DDLDTRLLRAMITKGIIEPISDS++NLVNA
Sbjct: 421 EKLGRLAVQLASGGKGVQSIRVVYRSARDRDDLDTRLLRAMITKGIIEPISDSYVNLVNA 480

Query: 509 DFTAKQKGLRISEERVLVDAPPEFPVESIQIVVSEVESKFASAVTEKGEVVIEGKVKYGV 568
           DF AKQKGLRISEER++VD+ PE+PV+SIQ+ +  VES FA AV++ G++ IEGKVKYGV
Sbjct: 481 DFIAKQKGLRISEERMVVDSSPEYPVDSIQVQILNVESNFAGAVSDAGDISIEGKVKYGV 540

Query: 569 PHLRRVGSFDVDVSLEGNLILCRQVDQPGMIGQVGNILGENNVNVNFMSVGRTLRRKRAI 628
           PHL  VGSF VDVSLEGNLILCRQVDQPGMIGQVGNILGE NVNVNFMSVGRT+ RK+AI
Sbjct: 541 PHLTCVGSFGVDVSLEGNLILCRQVDQPGMIGQVGNILGEQNVNVNFMSVGRTVLRKQAI 600

Query: 629 MAIGMDEEPNKDTLKKIGQVPAIEEFVFLNL 637
           MAIG+DEEP+  TL++IG V AIEEFVFL L
Sbjct: 601 MAIGVDEEPDNKTLERIGGVSAIEEFVFLKL 624

BLAST of Cucsa.192110 vs. Swiss-Prot
Match: SERA1_ARATH (D-3-phosphoglycerate dehydrogenase 1, chloroplastic OS=Arabidopsis thaliana GN=PGDH1 PE=1 SV=1)

HSP 1 Score: 860.1 bits (2221), Expect = 1.5e-248
Identity = 444/598 (74.25%), Postives = 518/598 (86.62%), Query Frame = 1

Query: 47  SSSSSSSSSSSSNRCSFIPLRSVLKTLESSAFVSQNSDLLD--------PTSPNDRSTIL 106
           ++SSS + +++S R   +  RS L +  S AF S+  + L          T    + TIL
Sbjct: 6   AASSSIAVATNSLRNVTLSSRSPLPSAISVAFPSRGRNTLQRRLVLVSCSTGDGSKPTIL 65

Query: 107 VAEKLGEAGLELLRSFGNVVCAYDLSPEELCAKISSCDALIVRSGTKVNRQVFEAAKGRL 166
           VAEKLG+AG++LL    NV C+Y+++PEEL  KIS CDALIVRSGTKV R+VFE++ GRL
Sbjct: 66  VAEKLGDAGIKLLEDVANVDCSYNMTPEELNIKISLCDALIVRSGTKVGREVFESSHGRL 125

Query: 167 KVVGRAGVGIDNVDLQAATEFGCLVVNAPTANTIAAAEHGIALLTSMARNVAQADASMKA 226
           KVVGRAGVGIDNVDL AATEFGCLVVNAPTANTIAAAEHGIAL+ +MARNVAQADAS+KA
Sbjct: 126 KVVGRAGVGIDNVDLSAATEFGCLVVNAPTANTIAAAEHGIALMAAMARNVAQADASVKA 185

Query: 227 GKWERNKYVGVSLVGKTLAVMGFGKVGSEVARRAKGLGMQVIAHDPYAPVDRARAIGVEL 286
           G+W+RNKYVGVSLVGKTLAV+GFGKVG+EVARRAKGLGM+VIAHDPYAP DRA AIGV+L
Sbjct: 186 GEWKRNKYVGVSLVGKTLAVLGFGKVGTEVARRAKGLGMRVIAHDPYAPADRAHAIGVDL 245

Query: 287 VSFNQAISTADFISLHMPLTPTTSKVFNDDTFGLMKKGARLINVARGGVIDEDALVRALD 346
           VSF++A++TADFISLHMPLTPTTSK+ ND+TF  MKKG R++NVARGGVIDEDALVRALD
Sbjct: 246 VSFDEALATADFISLHMPLTPTTSKILNDETFAKMKKGVRIVNVARGGVIDEDALVRALD 305

Query: 347 SGAVAQAALDVFVEEPPPKDSKLVQHKNVTVTPHLGASTKEAQEGVAIEIAEAVVGALNG 406
           +G VAQAALDVF +EPP KDSKLVQH+ VTVTPHLGAST EAQEGVAIEIAEAVVGALNG
Sbjct: 306 AGIVAQAALDVFTKEPPAKDSKLVQHERVTVTPHLGASTMEAQEGVAIEIAEAVVGALNG 365

Query: 407 ELSATAVNAPMVPPEVLSELAPYVVLAEKLGRLAVQLVAGGSGIKSVKVVYRSGRAPDDL 466
           EL+ATAVNAPMV  EVL+EL PYVVLAEKLGRLAVQLVAGGSG+K+ K+ Y S RA DDL
Sbjct: 366 ELAATAVNAPMVSAEVLTELKPYVVLAEKLGRLAVQLVAGGSGVKNAKITYASARATDDL 425

Query: 467 DTRLLRAMITKGIIEPISDSHINLVNADFTAKQKGLRISEERVLVDAPPEFPVESIQIVV 526
           DTRLLRAMITKGIIEPISD ++NLVNADFTAKQ+GLR+SEERVL+D  PE P+E+I + +
Sbjct: 426 DTRLLRAMITKGIIEPISDVYVNLVNADFTAKQRGLRLSEERVLLDGSPESPLETITVQL 485

Query: 527 SEVESKFASAVTEKGEVVIEGKVKYGVPHLRRVGSFDVDVSLEGNLILCRQVDQPGMIGQ 586
           S VESKFAS+++E GEV +EGKVK GVPHL +VGSF+VDV+LEG++ILCRQVDQPGMIG 
Sbjct: 486 SNVESKFASSLSESGEVKVEGKVKDGVPHLTKVGSFEVDVTLEGSIILCRQVDQPGMIGT 545

Query: 587 VGNILGENNVNVNFMSVGRTLRRKRAIMAIGMDEEPNKDTLKKIGQVPAIEEFVFLNL 637
           VG+ILGE+NVNVNFMSVGR   RK+AIMAIG+D+ P+K+TLKKIG++PA+EEFVFL L
Sbjct: 546 VGSILGESNVNVNFMSVGRIAPRKQAIMAIGVDDIPSKETLKKIGEIPAVEEFVFLKL 603

BLAST of Cucsa.192110 vs. Swiss-Prot
Match: SERA3_ARATH (D-3-phosphoglycerate dehydrogenase 3, chloroplastic OS=Arabidopsis thaliana GN=PGDH3 PE=1 SV=1)

HSP 1 Score: 820.5 bits (2118), Expect = 1.3e-236
Identity = 423/587 (72.06%), Postives = 495/587 (84.33%), Query Frame = 1

Query: 55  SSSSNRCSFIPLRSVLKTLESSAF-VSQNSDLLDPTSPND----RSTILVAEKLGEAGLE 114
           ++S N  S     S L T  SS F + Q   ++  TS +     + TILV EKLG+AG++
Sbjct: 2   ATSLNLSSIFSSSSRLVTTPSSVFPIRQRRRIILVTSSSSGGGGKPTILVTEKLGQAGID 61

Query: 115 LLRSFGNVVCAYDLSPEELCAKISSCDALIVRSGTKVNRQVFEAAKGRLKVVGRAGVGID 174
           LL+ + NV C+YDLS EELC KIS CDALIVRSGTKV R VFE+++GRLKVVGRAGVGID
Sbjct: 62  LLKKYANVDCSYDLSLEELCTKISLCDALIVRSGTKVGRDVFESSRGRLKVVGRAGVGID 121

Query: 175 NVDLQAATEFGCLVVNAPTANTIAAAEHGIALLTSMARNVAQADASMKAGKWERNKYVGV 234
           NVDL AATE+GCLVVNAPTANT+AAAEHGIALLT+MARN+AQADAS+KAGKW RNKYVGV
Sbjct: 122 NVDLAAATEYGCLVVNAPTANTVAAAEHGIALLTAMARNIAQADASIKAGKWTRNKYVGV 181

Query: 235 SLVGKTLAVMGFGKVGSEVARRAKGLGMQVIAHDPYAPVDRARAIGVELVSFNQAISTAD 294
           SLVGKTLAV+GFGKVGSEVARRA+GLGM VI HDPYAP DRARAIGVELVSF  AISTAD
Sbjct: 182 SLVGKTLAVLGFGKVGSEVARRARGLGMHVITHDPYAPADRARAIGVELVSFEVAISTAD 241

Query: 295 FISLHMPLTPTTSKVFNDDTFGLMKKGARLINVARGGVIDEDALVRALDSGAVAQAALDV 354
           FISLH+PLT  TSK+ ND TF +MKKG R++NVARGGVIDE+AL+RALDSG VAQAALDV
Sbjct: 242 FISLHLPLTAATSKMMNDVTFAMMKKGVRIVNVARGGVIDEEALLRALDSGIVAQAALDV 301

Query: 355 FVEEPPPKDSKLVQHKNVTVTPHLGASTKEAQEGVAIEIAEAVVGALNGELSATAVNAPM 414
           F  EPP KD+KLV H++VT TPHLGAST EAQEGV+IE+AEAV+GAL GEL+ATAVNAPM
Sbjct: 302 FTVEPPVKDNKLVLHESVTATPHLGASTMEAQEGVSIEVAEAVIGALRGELAATAVNAPM 361

Query: 415 VPPEVLSELAPYVVLAEKLGRLAVQLVAGGSGIKSVKVVYRSGRAPDDLDTRLLRAMITK 474
           VP EVL EL PYVVLAEKLGRLAVQLV GGSG+ +VKV Y S RAPDDLDTRLLRAM+ K
Sbjct: 362 VPLEVLRELKPYVVLAEKLGRLAVQLVTGGSGVNAVKVTYASSRAPDDLDTRLLRAMVIK 421

Query: 475 GIIEPISDSHINLVNADFTAKQKGLRISEERVLVDAPPEFPVESIQIVVSEVESKFASAV 534
           GIIEPIS   INLVN+D+ AKQ+G++ISEER+++D  PE P+E I + ++ VES+FASA+
Sbjct: 422 GIIEPISSVFINLVNSDYIAKQRGVKISEERMVLDGSPENPIEYITVRIANVESRFASAL 481

Query: 535 TEKGEVVIEGKVKYGVPHLRRVGSFDVDVSLEGNLILCRQVDQPGMIGQVGNILGENNVN 594
           +E GE+ +EG+VK GVP L +VG F VDVSLEG++ILCRQVDQPGMIG+V +ILG+ NVN
Sbjct: 482 SESGEIKVEGRVKQGVPSLTKVGLFGVDVSLEGSVILCRQVDQPGMIGKVASILGDENVN 541

Query: 595 VNFMSVGRTLRRKRAIMAIGMDEEPNKDTLKKIGQVPAIEEFVFLNL 637
           V+FMSVGR    K+A+MAIG+DE+P+K+TLKKIG +PAIEEFVFL L
Sbjct: 542 VSFMSVGRIAPGKQAVMAIGVDEQPSKETLKKIGDIPAIEEFVFLKL 588

BLAST of Cucsa.192110 vs. Swiss-Prot
Match: SERA_METJA (D-3-phosphoglycerate dehydrogenase OS=Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440) GN=serA PE=3 SV=1)

HSP 1 Score: 396.7 bits (1018), Expect = 4.7e-109
Identity = 222/546 (40.66%), Postives = 327/546 (59.89%), Query Frame = 1

Query: 97  ILVAEKLGEAGLELLRSFGNVVCAYDLSPEELCAKISSCDALIVRSGTKVNRQVFEAAKG 156
           ILV + L E  +++L   G V  A  L+ EEL  KI   D L+VRSGTKV R V E A+ 
Sbjct: 4   ILVTDPLHEDAIKILEEVGEVEVATGLTKEELLEKIKDADVLVVRSGTKVTRDVIEKAE- 63

Query: 157 RLKVVGRAGVGIDNVDLQAATEFGCLVVNAPTANTIAAAEHGIALLTSMARNVAQADASM 216
           +LKV+GRAGVG+DN+D++AATE G +VVNAP A++I+ AE  + L+ + ARN+ QA AS+
Sbjct: 64  KLKVIGRAGVGVDNIDVEAATEKGIIVVNAPDASSISVAELTMGLMLAAARNIPQATASL 123

Query: 217 KAGKWERNKYVGVSLVGKTLAVMGFGKVGSEVARRAKGLGMQVIAHDPYAPVDRARAIGV 276
           K G+W+R ++ G+ L GKTL V+G G++G +V +RAK  GM +I +DPY P + A ++GV
Sbjct: 124 KRGEWDRKRFKGIELYGKTLGVIGLGRIGQQVVKRAKAFGMNIIGYDPYIPKEVAESMGV 183

Query: 277 ELV-SFNQAISTADFISLHMPLTPTTSKVFNDDTFGLMKKGARLINVARGGVIDEDALVR 336
           ELV   N+    ADFI+LH+PLTP T  +   +   LMKK A ++N ARGG+IDE AL  
Sbjct: 184 ELVDDINELCKRADFITLHVPLTPKTRHIIGREQIALMKKNAIIVNCARGGLIDEKALYE 243

Query: 337 ALDSGAVAQAALDVFVEEPPPKDSKLVQHKNVTVTPHLGASTKEAQEGVAIEIAEAVVGA 396
           AL  G +  AALDVF EE PPKD+ L+   NV  TPH GAST+EAQ+     +AE +   
Sbjct: 244 ALKEGKIRAAALDVF-EEEPPKDNPLLTLDNVIGTPHQGASTEEAQKAAGTIVAEQIKKV 303

Query: 397 LNGELSATAVNAPMVPPEVLSELAPYVVLAEKLGRLAVQLVAGGSGIKSVKVVYRSGRAP 456
           L GEL+   VN P +P E L +L PY++LAE LG + +Q++ G   +  V+++Y    A 
Sbjct: 304 LRGELAENVVNMPNIPQEKLGKLKPYMLLAEMLGNIVMQVLDG--SVNRVELIYSGELAK 363

Query: 457 DDLDTRLLRAMITKGIIEPISDSHINLVNADFTAKQKGLRISEERVLVDAPPEFPVESIQ 516
           +  D  L++    KG++ PI  + INLVNA   AK + + + E                 
Sbjct: 364 EKTD--LIKRAFLKGLLSPILLAGINLVNAPIIAKNRNINVVES---------------- 423

Query: 517 IVVSEVESKFASAV-----TEKGEVVIEGKVKYGVPHLRRVGSFDVDVSLEGNLILCRQV 576
              S  E K+ +A+     ++K +  I G +    P +  V  ++V    EG L + + +
Sbjct: 424 ---STSEEKYGNAIKITAESDKKKFSIVGAIINNKPVILEVDGYEVSFIPEGVLAIIKHI 483

Query: 577 DQPGMIGQVGNILGENNVNVNFMSVGRTLRRKRAIMAIGMDEEPNKDTLKKIGQVPAIEE 636
           D+PG IG+V   LG+  +N+  M VGR      ++M + +D    ++ ++KI ++P I++
Sbjct: 484 DRPGTIGRVCITLGDYGINIASMQVGRKEPGGESVMLLNLDHTVPEEVIEKIKEIPNIKD 524

BLAST of Cucsa.192110 vs. Swiss-Prot
Match: SERA_METTH (D-3-phosphoglycerate dehydrogenase OS=Methanothermobacter thermautotrophicus (strain ATCC 29096 / DSM 1053 / JCM 10044 / NBRC 100330 / Delta H) GN=serA PE=3 SV=1)

HSP 1 Score: 373.6 bits (958), Expect = 4.2e-102
Identity = 202/543 (37.20%), Postives = 319/543 (58.75%), Query Frame = 1

Query: 94  RSTILVAEKLGEAGLELLRSFGNVVCAYDLSPEELCAKISSCDALIVRSGTKVNRQVFEA 153
           R  +L+A+ + E G+  L     VV    ++PEEL   I   DA++VRS TKV R+V EA
Sbjct: 3   RMKVLIADSINEKGISELEEVAEVVVNTTITPEELLDAIKDFDAIVVRSRTKVTREVIEA 62

Query: 154 AKGRLKVVGRAGVGIDNVDLQAATEFGCLVVNAPTANTIAAAEHGIALLTSMARNVAQAD 213
           A  RLK++ RAGVG+DNVD++AAT+ G +V+NAP + +I  AEH I L+ ++AR +A AD
Sbjct: 63  AP-RLKIIARAGVGVDNVDVKAATDRGIMVINAPESTSITVAEHSIGLMLALARKIAIAD 122

Query: 214 ASMKAGKWERNKYVGVSLVGKTLAVMGFGKVGSEVARRAKGLGMQVIAHDPYAPVDRARA 273
            S+K GKWE+N+++G+ L GKTL ++G G++GS+V  R K  GM ++ +DPY   + A  
Sbjct: 123 RSVKEGKWEKNRFMGIELNGKTLGIIGMGRIGSQVVVRTKAFGMDIMVYDPYISKEAAEE 182

Query: 274 IGVELVSFNQAISTADFISLHMPLTPTTSKVFNDDTFGLMKKGARLINVARGGVIDEDAL 333
           +GV +      +  +D +++H+PLTP T  + ++D F LMK  A ++N ARGG+IDEDAL
Sbjct: 183 MGVTVTDLETLLRESDIVTIHVPLTPETRHLISEDEFKLMKDTAFIVNCARGGIIDEDAL 242

Query: 334 VRALDSGAVAQAALDVFVEEPPPKDSKLVQHKNVTVTPHLGASTKEAQEGVAIEIAEAVV 393
            RAL  G +A AALDVF EE PP+ S L++ +NV +TPH+GAST EAQ   AI +A  + 
Sbjct: 243 YRALKDGEIAGAALDVF-EEEPPEGSPLLELENVVLTPHIGASTSEAQRDAAIIVANEIK 302

Query: 394 GALNGELSATAVNAPMVPPEVLSELAPYVVLAEKLGRLAVQLVAGGSGIKSVKVVYRSGR 453
               G      +N P++  E    L PY+ LAEK+G +  Q + G   I+ + V Y    
Sbjct: 303 TVFQGGAPRNVLNMPVMDSETYKSLKPYIELAEKMGAIIAQALPG--NIEKLDVTYCGEL 362

Query: 454 APDDLDTRLLRAMITKGIIEPISDSHINLVNADFTAKQKGLRISEERVLVDAPPEFPVES 513
           A    D  +L   + + I+ PI    +NL+NA   AK++G+ ++E R           + 
Sbjct: 363 AEMQFD--ILTRTMLQAILNPILTEPVNLINAPSIAKKRGIMVTEAR-------RSESDG 422

Query: 514 IQIVVSEVESKFASAVTEKGEVVIEGKVKYGVPHLRRVGSFDVDVSLEGNLILCRQVDQP 573
            + ++       A+A +++G   +E       P +  +  + VDV  EG +I+ R  D P
Sbjct: 423 YRSII------IATAESDRGGFSVEA-THIKEPTIIGINGYRVDVKPEGTMIIARYRDLP 482

Query: 574 GMIGQVGNILGENNVNVNFMSVGRTLRRKRAIMAIGMDEEPNKDTLKKIGQVPAIEEFVF 633
           G IG +G  LG++ +N+  M VGR      A+M + +D+    + ++++ ++  +++ V 
Sbjct: 483 GTIGAIGTKLGQHGINIATMQVGRKEIGGEAVMVLKVDQSVPAEVIEEVKKLDNVDDAVA 525

Query: 634 LNL 637
           + +
Sbjct: 543 IEI 525

BLAST of Cucsa.192110 vs. TrEMBL
Match: A0A0A0LC35_CUCSA (D-3-phosphoglycerate dehydrogenase OS=Cucumis sativus GN=Csa_3G651740 PE=3 SV=1)

HSP 1 Score: 1205.7 bits (3118), Expect = 0.0e+00
Identity = 632/636 (99.37%), Postives = 632/636 (99.37%), Query Frame = 1

Query: 1   MASSSSNSLFSSPHFTSFNRSFHSKPPLLSFSTSSVSLNPRPLHSFSSSSSSSSSSSSNR 60
           MASSSSNSLFSSPHFTSFNRSFHSKPPLLSFSTSSVSLNPRPLHS    SSSSSSSSSNR
Sbjct: 1   MASSSSNSLFSSPHFTSFNRSFHSKPPLLSFSTSSVSLNPRPLHS----SSSSSSSSSNR 60

Query: 61  CSFIPLRSVLKTLESSAFVSQNSDLLDPTSPNDRSTILVAEKLGEAGLELLRSFGNVVCA 120
           CSFIPLRSVLKTLESSAFVSQNSDLLDPTSPNDRSTILVAEKLGEAGLELLRSFGNVVCA
Sbjct: 61  CSFIPLRSVLKTLESSAFVSQNSDLLDPTSPNDRSTILVAEKLGEAGLELLRSFGNVVCA 120

Query: 121 YDLSPEELCAKISSCDALIVRSGTKVNRQVFEAAKGRLKVVGRAGVGIDNVDLQAATEFG 180
           YDLSPEELCAKISSCDALIVRSGTKVNRQVFEAAKGRLKVVGRAGVGIDNVDLQAATEFG
Sbjct: 121 YDLSPEELCAKISSCDALIVRSGTKVNRQVFEAAKGRLKVVGRAGVGIDNVDLQAATEFG 180

Query: 181 CLVVNAPTANTIAAAEHGIALLTSMARNVAQADASMKAGKWERNKYVGVSLVGKTLAVMG 240
           CLVVNAPTANTIAAAEHGIALLTSMARNVAQADASMKAGKWERNKYVGVSLVGKTLAVMG
Sbjct: 181 CLVVNAPTANTIAAAEHGIALLTSMARNVAQADASMKAGKWERNKYVGVSLVGKTLAVMG 240

Query: 241 FGKVGSEVARRAKGLGMQVIAHDPYAPVDRARAIGVELVSFNQAISTADFISLHMPLTPT 300
           FGKVGSEVARRAKGLGMQVIAHDPYAPVDRARAIGVELVSFNQAISTADFISLHMPLTPT
Sbjct: 241 FGKVGSEVARRAKGLGMQVIAHDPYAPVDRARAIGVELVSFNQAISTADFISLHMPLTPT 300

Query: 301 TSKVFNDDTFGLMKKGARLINVARGGVIDEDALVRALDSGAVAQAALDVFVEEPPPKDSK 360
           TSKVFNDDTFGLMKKGARLINVARGGVIDEDALVRALDSGAVAQAALDVFVEEPPPKDSK
Sbjct: 301 TSKVFNDDTFGLMKKGARLINVARGGVIDEDALVRALDSGAVAQAALDVFVEEPPPKDSK 360

Query: 361 LVQHKNVTVTPHLGASTKEAQEGVAIEIAEAVVGALNGELSATAVNAPMVPPEVLSELAP 420
           LVQHKNVTVTPHLGASTKEAQEGVAIEIAEAVVGALNGELSATAVNAPMVPPEVLSELAP
Sbjct: 361 LVQHKNVTVTPHLGASTKEAQEGVAIEIAEAVVGALNGELSATAVNAPMVPPEVLSELAP 420

Query: 421 YVVLAEKLGRLAVQLVAGGSGIKSVKVVYRSGRAPDDLDTRLLRAMITKGIIEPISDSHI 480
           YVVLAEKLGRLAVQLVAGGSGIKSVKVVYRSGRAPDDLDTRLLRAMITKGIIEPISDSHI
Sbjct: 421 YVVLAEKLGRLAVQLVAGGSGIKSVKVVYRSGRAPDDLDTRLLRAMITKGIIEPISDSHI 480

Query: 481 NLVNADFTAKQKGLRISEERVLVDAPPEFPVESIQIVVSEVESKFASAVTEKGEVVIEGK 540
           NLVNADFTAKQKGLRISEERVLVDAPPEFPVESIQIVVSEVESKFASAVTEKGEVVIEGK
Sbjct: 481 NLVNADFTAKQKGLRISEERVLVDAPPEFPVESIQIVVSEVESKFASAVTEKGEVVIEGK 540

Query: 541 VKYGVPHLRRVGSFDVDVSLEGNLILCRQVDQPGMIGQVGNILGENNVNVNFMSVGRTLR 600
           VKYGVPHLRRVGSFDVDVSLEGNLILCRQVDQPGMIGQVGNILGENNVNVNFMSVGRTLR
Sbjct: 541 VKYGVPHLRRVGSFDVDVSLEGNLILCRQVDQPGMIGQVGNILGENNVNVNFMSVGRTLR 600

Query: 601 RKRAIMAIGMDEEPNKDTLKKIGQVPAIEEFVFLNL 637
           RKRAIMAIGMDEEPNKDTLKKIGQVPAIEEFVFLNL
Sbjct: 601 RKRAIMAIGMDEEPNKDTLKKIGQVPAIEEFVFLNL 632

BLAST of Cucsa.192110 vs. TrEMBL
Match: M1AIT2_SOLTU (D-3-phosphoglycerate dehydrogenase OS=Solanum tuberosum GN=PGSC0003DMG400009159 PE=3 SV=1)

HSP 1 Score: 943.7 bits (2438), Expect = 1.1e-271
Identity = 508/641 (79.25%), Postives = 564/641 (87.99%), Query Frame = 1

Query: 2   ASSSSNSLFSSPHFTSFNRSFHSKPPLLSF-STSSVSLNPRPLHSFSSSSS-----SSSS 61
           A++  NSL SS   +S N   H+KP  LSF   SS   + + LHS S+SSS     SSSS
Sbjct: 11  ATAHLNSLLSSS--SSVN---HNKPSNLSFLHASSNPSSIKLLHSVSASSSFPPSISSSS 70

Query: 62  SSSNRCSFIPLRSVLKTLESSAFVSQNSDLLDPTSPNDRSTILVAEKLGEAGLELLRSFG 121
           S+++ C+      VLKT+ES A +S + DL    S + + TILV+EKLGEAGL+LLRSFG
Sbjct: 71  STTSICN------VLKTVES-ADISLSRDLHGVVSTS-KPTILVSEKLGEAGLDLLRSFG 130

Query: 122 NVVCAYDLSPEELCAKISSCDALIVRSGTKVNRQVFEAAKGRLKVVGRAGVGIDNVDLQA 181
           NV C+YDLSP++LC KIS CDALIVRSGTKV R+VFEAA+GRLKVVGRAGVGIDNVDLQA
Sbjct: 131 NVDCSYDLSPQDLCTKISLCDALIVRSGTKVTREVFEAAQGRLKVVGRAGVGIDNVDLQA 190

Query: 182 ATEFGCLVVNAPTANTIAAAEHGIALLTSMARNVAQADASMKAGKWERNKYVGVSLVGKT 241
           ATEFGCLVVNAPTANTIAAAEHGIALLTSMARNVAQ+DASMKAGKW R+KYVGVSLVGKT
Sbjct: 191 ATEFGCLVVNAPTANTIAAAEHGIALLTSMARNVAQSDASMKAGKWLRSKYVGVSLVGKT 250

Query: 242 LAVMGFGKVGSEVARRAKGLGMQVIAHDPYAPVDRARAIGVELVSFNQAISTADFISLHM 301
           LA+MGFGKVGSEVARRAKGLGM VIAHDPYAP DRARAIGV+LVSF QAISTADFISLHM
Sbjct: 251 LAIMGFGKVGSEVARRAKGLGMHVIAHDPYAPADRARAIGVDLVSFEQAISTADFISLHM 310

Query: 302 PLTPTTSKVFNDDTFGLMKKGARLINVARGGVIDEDALVRALDSGAVAQAALDVFVEEPP 361
           PLTP T+KVFNDDTF  MKKG RLINVARGGVIDEDALVRALDSG VAQAALDVF  EPP
Sbjct: 311 PLTPATNKVFNDDTFAKMKKGVRLINVARGGVIDEDALVRALDSGIVAQAALDVFTVEPP 370

Query: 362 PKDSKLVQHKNVTVTPHLGASTKEAQEGVAIEIAEAVVGALNGELSATAVNAPMVPPEVL 421
           PKDSKLVQH+NVTVTPHLGASTKEAQEGVAIEIAEAVVGALNGELSATAVNAPMVPPEVL
Sbjct: 371 PKDSKLVQHENVTVTPHLGASTKEAQEGVAIEIAEAVVGALNGELSATAVNAPMVPPEVL 430

Query: 422 SELAPYVVLAEKLGRLAVQLVAGGSGIKSVKVVYRSGRAPDDLDTRLLRAMITKGIIEPI 481
           SELAPYVVLAEKLGRLAVQLV GGSGI+SVKVVY+S R PD LDTRLLRAM+TKGIIEPI
Sbjct: 431 SELAPYVVLAEKLGRLAVQLVTGGSGIQSVKVVYKSARDPDSLDTRLLRAMVTKGIIEPI 490

Query: 482 SDSHINLVNADFTAKQKGLRISEERVLVDAPPEFPVESIQIVVSEVESKFASAVTEKGEV 541
           SD+ INLVNADF+AKQKGLRISEER++VD+ PE+PVESIQ+ +S V+S+FASA++E G +
Sbjct: 491 SDTIINLVNADFSAKQKGLRISEERIIVDSSPEYPVESIQVQISNVQSRFASALSENGNI 550

Query: 542 VIEGKVKYGVPHLRRVGSFDVDVSLEGNLILCRQVDQPGMIGQVGNILGENNVNVNFMSV 601
            IEGKVKYGVPHL RVG F VDVSLEGNLILC+QVDQPGMIG+VGNILGE+NVNV+FMSV
Sbjct: 551 SIEGKVKYGVPHLTRVGPFSVDVSLEGNLILCKQVDQPGMIGKVGNILGESNVNVSFMSV 610

Query: 602 GRTLRRKRAIMAIGMDEEPNKDTLKKIGQVPAIEEFVFLNL 637
           GRT++ K+AIMAIG+DEEP+KDT KKIG+V A+EEFVFL L
Sbjct: 611 GRTVKGKQAIMAIGVDEEPDKDTQKKIGEVSAVEEFVFLKL 638

BLAST of Cucsa.192110 vs. TrEMBL
Match: A0A022Q288_ERYGU (D-3-phosphoglycerate dehydrogenase OS=Erythranthe guttata GN=MIMGU_mgv1a002844mg PE=3 SV=1)

HSP 1 Score: 943.3 bits (2437), Expect = 1.5e-271
Identity = 496/635 (78.11%), Postives = 552/635 (86.93%), Query Frame = 1

Query: 3   SSSSNSLFS-SPHFTSFNRSFHSKPPLLSFSTSSVSLNPRPLHSFSSSSSSSSSSSSNRC 62
           SS++N L + +P F    ++ HS P  LSFS SS+SL      SFS++ ++ +S SS   
Sbjct: 7   SSAANKLTNQTPSFQPQEKNSHSSPTCLSFSNSSISLQKL---SFSTNFTNPNSRSSTAI 66

Query: 63  SFIPLRSVLKTLESSAFVSQNSDLLDPTSPNDRSTILVAEKLGEAGLELLRSFGNVVCAY 122
           +      VLKT E+S  VS+  D  +P   + + TILV+EKLGEAGL+LLRSFGNV C Y
Sbjct: 67  N------VLKTAEASESVSRTRDP-EPVGKSTKPTILVSEKLGEAGLDLLRSFGNVECLY 126

Query: 123 DLSPEELCAKISSCDALIVRSGTKVNRQVFEAAKGRLKVVGRAGVGIDNVDLQAATEFGC 182
            LS E+LCAKIS CDALIVRSGTKV RQVFEAAKG+LKVVGRAGVGIDNVDLQAATEFGC
Sbjct: 127 SLSQEDLCAKISECDALIVRSGTKVTRQVFEAAKGKLKVVGRAGVGIDNVDLQAATEFGC 186

Query: 183 LVVNAPTANTIAAAEHGIALLTSMARNVAQADASMKAGKWERNKYVGVSLVGKTLAVMGF 242
           LVVNAPTANTIAAAEHGIALL  MARNVAQADASMKAGKW+RNKYVGVSLVGKTLAVMGF
Sbjct: 187 LVVNAPTANTIAAAEHGIALLAGMARNVAQADASMKAGKWQRNKYVGVSLVGKTLAVMGF 246

Query: 243 GKVGSEVARRAKGLGMQVIAHDPYAPVDRARAIGVELVSFNQAISTADFISLHMPLTPTT 302
           GKVGSEVARRAKGLGM VIAHDPYAP DRARA+G ELVSF+QAISTADFISLHMPLTP T
Sbjct: 247 GKVGSEVARRAKGLGMHVIAHDPYAPADRARALGAELVSFDQAISTADFISLHMPLTPAT 306

Query: 303 SKVFNDDTFGLMKKGARLINVARGGVIDEDALVRALDSGAVAQAALDVFVEEPPPKDSKL 362
           +K+ NDDTF  MKKG R+INVARGGVIDEDALVRALD+G VAQAALDVF  EPPP+DSKL
Sbjct: 307 NKILNDDTFAKMKKGVRIINVARGGVIDEDALVRALDNGIVAQAALDVFTVEPPPQDSKL 366

Query: 363 VQHKNVTVTPHLGASTKEAQEGVAIEIAEAVVGALNGELSATAVNAPMVPPEVLSELAPY 422
           VQH+NVTVTPHLGASTKEAQEGVA+EIAEAVVGAL GELSATAVNAPMVPPEVLSELAPY
Sbjct: 367 VQHENVTVTPHLGASTKEAQEGVAVEIAEAVVGALRGELSATAVNAPMVPPEVLSELAPY 426

Query: 423 VVLAEKLGRLAVQLVAGGSGIKSVKVVYRSGRAPDDLDTRLLRAMITKGIIEPISDSHIN 482
           V LAEKLGRLAVQLVAGGSGI+SVKV Y S R PD+LDTRLLRAMITKGIIEPISD+HIN
Sbjct: 427 VALAEKLGRLAVQLVAGGSGIQSVKVAYSSARDPDNLDTRLLRAMITKGIIEPISDAHIN 486

Query: 483 LVNADFTAKQKGLRISEERVLVDAPPEFPVESIQIVVSEVESKFASAVTEKGEVVIEGKV 542
           LVNADF AKQKGLR+SEERV VD+ PE P+ESIQ+ +S V SKFASA+++ G + +EGKV
Sbjct: 487 LVNADFIAKQKGLRLSEERVFVDSSPENPIESIQVQISNVGSKFASALSDNGNISVEGKV 546

Query: 543 KYGVPHLRRVGSFDVDVSLEGNLILCRQVDQPGMIGQVGNILGENNVNVNFMSVGRTLRR 602
           KYGVPHL RVG F VDVSLEGNLILCRQVDQPGMIGQVG+ILGE+NVNV+FMSVGRTL+R
Sbjct: 547 KYGVPHLTRVGPFSVDVSLEGNLILCRQVDQPGMIGQVGSILGESNVNVSFMSVGRTLKR 606

Query: 603 KRAIMAIGMDEEPNKDTLKKIGQVPAIEEFVFLNL 637
            +A+MAIG+DEEP+K+TLKKIG+V AIEEFVFL+L
Sbjct: 607 TKAVMAIGVDEEPDKETLKKIGEVAAIEEFVFLDL 631

BLAST of Cucsa.192110 vs. TrEMBL
Match: A0A0V0IKH0_SOLCH (D-3-phosphoglycerate dehydrogenase OS=Solanum chacoense PE=3 SV=1)

HSP 1 Score: 943.3 bits (2437), Expect = 1.5e-271
Identity = 506/640 (79.06%), Postives = 560/640 (87.50%), Query Frame = 1

Query: 1   MASSSSNSLFSSPHFTSFNRSF---HSKPPLLSF-STSSVSLNPRPLHSFSSSSSSSSSS 60
           MAS+ S    ++ H  S   S    H+KP  LSF   SS   + + LHS S S S SSSS
Sbjct: 1   MASTPSPHTAATAHLNSLLSSSSVNHNKPSNLSFLHASSNPSSIKLLHSSSFSPSISSSS 60

Query: 61  SSNRCSFIPLRSVLKTLESSAFVSQNSDLLDPTSPNDRSTILVAEKLGEAGLELLRSFGN 120
           S+       + +VLKT+ES A +S + DL    S + + TILV+EKLGEAGL+LLRSFGN
Sbjct: 61  STTA-----ICNVLKTVES-ADISLSRDLHGVVSTS-KPTILVSEKLGEAGLDLLRSFGN 120

Query: 121 VVCAYDLSPEELCAKISSCDALIVRSGTKVNRQVFEAAKGRLKVVGRAGVGIDNVDLQAA 180
           V C+YDLSP++LCAKIS CDALIVRSGTKV R+VFEAA+GRLKVVGRAGVGIDNVDLQAA
Sbjct: 121 VDCSYDLSPQDLCAKISLCDALIVRSGTKVTREVFEAAQGRLKVVGRAGVGIDNVDLQAA 180

Query: 181 TEFGCLVVNAPTANTIAAAEHGIALLTSMARNVAQADASMKAGKWERNKYVGVSLVGKTL 240
           TEFGCLVVNAPTANTIAAAEHGIALLTSMARNVAQ+DASMKAGKW R+KYVGVSLVGKTL
Sbjct: 181 TEFGCLVVNAPTANTIAAAEHGIALLTSMARNVAQSDASMKAGKWLRSKYVGVSLVGKTL 240

Query: 241 AVMGFGKVGSEVARRAKGLGMQVIAHDPYAPVDRARAIGVELVSFNQAISTADFISLHMP 300
           A+MGFGKVGSEVARRAKGLGM VIAHDPYAP DRARAIGV+LVSF QAISTADFISLHMP
Sbjct: 241 AIMGFGKVGSEVARRAKGLGMHVIAHDPYAPADRARAIGVDLVSFEQAISTADFISLHMP 300

Query: 301 LTPTTSKVFNDDTFGLMKKGARLINVARGGVIDEDALVRALDSGAVAQAALDVFVEEPPP 360
           LTP T+KVFNDDTF  MKKG RLINVARGGVIDEDALVRALDSG VAQAALDVF  EPPP
Sbjct: 301 LTPATNKVFNDDTFPKMKKGVRLINVARGGVIDEDALVRALDSGIVAQAALDVFTVEPPP 360

Query: 361 KDSKLVQHKNVTVTPHLGASTKEAQEGVAIEIAEAVVGALNGELSATAVNAPMVPPEVLS 420
           KDSKLVQH+NVTVTPHLGASTKEAQEGVAIEIAEAVVGALNGELSATAVNAPMVPPEVLS
Sbjct: 361 KDSKLVQHENVTVTPHLGASTKEAQEGVAIEIAEAVVGALNGELSATAVNAPMVPPEVLS 420

Query: 421 ELAPYVVLAEKLGRLAVQLVAGGSGIKSVKVVYRSGRAPDDLDTRLLRAMITKGIIEPIS 480
           ELAPYVVLAEKLGRLAVQLV GGSGI+SVKVVY+S R PD LDTRLLRAM+TKGIIEPIS
Sbjct: 421 ELAPYVVLAEKLGRLAVQLVTGGSGIQSVKVVYKSARDPDSLDTRLLRAMVTKGIIEPIS 480

Query: 481 DSHINLVNADFTAKQKGLRISEERVLVDAPPEFPVESIQIVVSEVESKFASAVTEKGEVV 540
           D+ INLVNADF+AKQKGLRISEER++VD+ PE+PVESIQ+ +S V+S+FASA++E G + 
Sbjct: 481 DTIINLVNADFSAKQKGLRISEERIIVDSSPEYPVESIQVQISNVQSRFASALSENGNIS 540

Query: 541 IEGKVKYGVPHLRRVGSFDVDVSLEGNLILCRQVDQPGMIGQVGNILGENNVNVNFMSVG 600
           IEGKVKYGVPHL RVG F VDVSLEGNLILC+QVDQPGMIG+VGNILGE+NVNV+FMSVG
Sbjct: 541 IEGKVKYGVPHLTRVGPFSVDVSLEGNLILCKQVDQPGMIGKVGNILGESNVNVSFMSVG 600

Query: 601 RTLRRKRAIMAIGMDEEPNKDTLKKIGQVPAIEEFVFLNL 637
           RT++ K+AIMAIG+DEEP+KDT KKIG+V A+EEFVFL L
Sbjct: 601 RTVKGKQAIMAIGVDEEPDKDTQKKIGEVSAVEEFVFLKL 633

BLAST of Cucsa.192110 vs. TrEMBL
Match: B9HK69_POPTR (D-3-phosphoglycerate dehydrogenase OS=Populus trichocarpa GN=POPTR_0008s01000g PE=3 SV=1)

HSP 1 Score: 943.0 bits (2436), Expect = 1.9e-271
Identity = 503/642 (78.35%), Postives = 560/642 (87.23%), Query Frame = 1

Query: 1   MASSSSNSLFSSPHFTSFNRSFH--SKPPLLSF--STSSVSLNPRPLHSFSSSSSSSSSS 60
           MASSS+  + + P  T+ + S    ++  LLSF  +T+   ++ +  HS +S  +S SSS
Sbjct: 1   MASSSTKPISTPPFTTTISSSSQPSTRSSLLSFLRNTTPTPISLKLSHSRNSFLNSHSSS 60

Query: 61  SSNRCSFIPLRSVLKTLESSAFVSQNSDL--LDPTSPNDRSTILVAEKLGEAGLELLRSF 120
           S +    + +++  KT+ES A  S+ S +   D  S   + TILV+EKLGEAGLELLRSF
Sbjct: 61  SRS----LSIKNATKTIES-AETSRVSKVGGQDADSQETKPTILVSEKLGEAGLELLRSF 120

Query: 121 GNVVCAYDLSPEELCAKISSCDALIVRSGTKVNRQVFEAAKGRLKVVGRAGVGIDNVDLQ 180
           G+V C+YDLS E+LC KI+SCDALIVRSGTKV RQVFEAAKG+LKVVGRAGVGIDNVDLQ
Sbjct: 121 GDVDCSYDLSQEDLCKKIASCDALIVRSGTKVTRQVFEAAKGKLKVVGRAGVGIDNVDLQ 180

Query: 181 AATEFGCLVVNAPTANTIAAAEHGIALLTSMARNVAQADASMKAGKWERNKYVGVSLVGK 240
           AATEFGCLVVNAPTANT+AAAEHGIALL +MARNVAQADASMKAG+W+RNKYVGVSLVGK
Sbjct: 181 AATEFGCLVVNAPTANTVAAAEHGIALLAAMARNVAQADASMKAGQWQRNKYVGVSLVGK 240

Query: 241 TLAVMGFGKVGSEVARRAKGLGMQVIAHDPYAPVDRARAIGVELVSFNQAISTADFISLH 300
           TLAVMGFGKVGSEVARRAKGLGMQVIAHDPYAP DRARAIGVELVSF+QAISTADFISLH
Sbjct: 241 TLAVMGFGKVGSEVARRAKGLGMQVIAHDPYAPADRARAIGVELVSFDQAISTADFISLH 300

Query: 301 MPLTPTTSKVFNDDTFGLMKKGARLINVARGGVIDEDALVRALDSGAVAQAALDVFVEEP 360
           MPLTP+T KVFNDDTF  +K G R+INVARGGVIDEDALVRALDSG VAQAALDVF EEP
Sbjct: 301 MPLTPSTEKVFNDDTFAKVKTGVRIINVARGGVIDEDALVRALDSGKVAQAALDVFTEEP 360

Query: 361 PPKDSKLVQHKNVTVTPHLGASTKEAQEGVAIEIAEAVVGALNGELSATAVNAPMVPPEV 420
           PPKDSKLVQH+ VTVTPHLGASTKEAQEGVAIEIAEAVVGAL GEL+ATAVNAPMVP EV
Sbjct: 361 PPKDSKLVQHERVTVTPHLGASTKEAQEGVAIEIAEAVVGALQGELAATAVNAPMVPAEV 420

Query: 421 LSELAPYVVLAEKLGRLAVQLVAGGSGIKSVKVVYRSGRAPDDLDTRLLRAMITKGIIEP 480
           LSELAPYVVLAEKLGRLAVQLVAGGSGIKS KVVYRS R PDDLDTRLLRAMITKGIIEP
Sbjct: 421 LSELAPYVVLAEKLGRLAVQLVAGGSGIKSAKVVYRSSRDPDDLDTRLLRAMITKGIIEP 480

Query: 481 ISDSHINLVNADFTAKQKGLRISEERVLVDAPPEFPVESIQIVVSEVESKFASAVTEKGE 540
           ISDS INLVNADFTAKQKGLRISEERV+VD  PEFP+ SIQ+ +S V+SKF S V+E G+
Sbjct: 481 ISDSFINLVNADFTAKQKGLRISEERVVVDTSPEFPIHSIQVQLSNVDSKFGSGVSEGGD 540

Query: 541 VVIEGKVKYGVPHLRRVGSFDVDVSLEGNLILCRQVDQPGMIGQVGNILGENNVNVNFMS 600
           + IEG+VKYG PHL RVGSF VDVSLEGNLILCRQVDQPGMIGQVGNILGE NVNV+FMS
Sbjct: 541 ISIEGRVKYGKPHLTRVGSFSVDVSLEGNLILCRQVDQPGMIGQVGNILGEQNVNVSFMS 600

Query: 601 VGRTLRRKRAIMAIGMDEEPNKDTLKKIGQVPAIEEFVFLNL 637
           VGRT++R++AIMAIG+DEEPN++TLKKIG+VPAIEEFVFL L
Sbjct: 601 VGRTVQRRKAIMAIGVDEEPNQETLKKIGEVPAIEEFVFLKL 637

BLAST of Cucsa.192110 vs. TAIR10
Match: AT1G17745.2 (AT1G17745.2 D-3-phosphoglycerate dehydrogenase)

HSP 1 Score: 862.1 bits (2226), Expect = 2.2e-250
Identity = 465/658 (70.67%), Postives = 528/658 (80.24%), Query Frame = 1

Query: 29  LSFSTSSVSLNPRPLHSFSSSSSSSSSSSSNRCSFIP---------------LRSVLKTL 88
           ++FS+S  S+      + +S  +S S S S+R + +P               + + LKT+
Sbjct: 1   MAFSSSCSSVK-----AVNSRWTSPSPSPSSRFAVLPAFLHRRYATSVKLTAISAALKTV 60

Query: 89  ESSAFVSQN--------SDLLDPTSPNDRSTILVAEKLGEAGLELLRSFGNVVCAYDLSP 148
           E +     N        SD  +PT P  R  ILV EKLGEAG+ LLR FG+V C+YDLSP
Sbjct: 61  EQTTLTEDNRFSTVGSDSDEYNPTLPKPR--ILVTEKLGEAGVNLLREFGDVDCSYDLSP 120

Query: 149 EELCAKISSCDALIVRSGTKVNRQVFEAAKGRLKVVGRAGVGIDNVDLQAATEFGCLVVN 208
           E+L  K++  DALIVRSGTKV R+VFEAAKGRLKVVGRAGVGIDNVDLQAATE GCLVVN
Sbjct: 121 EDLKKKVAESDALIVRSGTKVTREVFEAAKGRLKVVGRAGVGIDNVDLQAATEHGCLVVN 180

Query: 209 APTANTIAAAEHGIALLTSMARNVAQADASMKAG-----------KW------------- 268
           APTANT+AAAEHGIALL SMARNVAQADAS+KAG           +W             
Sbjct: 181 APTANTVAAAEHGIALLASMARNVAQADASIKAGTLNYLFLVLLLRWNCRQSKHQYTIET 240

Query: 269 ---ERNKYVGVSLVGKTLAVMGFGKVGSEVARRAKGLGMQVIAHDPYAPVDRARAIGVEL 328
              +R+KYVGVSLVGKTLAVMGFGKVG+EVARRAKGLGM VI+HDPYAP DRARA+GV+L
Sbjct: 241 ETEKRSKYVGVSLVGKTLAVMGFGKVGTEVARRAKGLGMTVISHDPYAPADRARALGVDL 300

Query: 329 VSFNQAISTADFISLHMPLTPTTSKVFNDDTFGLMKKGARLINVARGGVIDEDALVRALD 388
           VSF+QAISTADF+SLHMPLTP T KVFND+TF  MKKG RLINVARGGVIDEDALVRALD
Sbjct: 301 VSFDQAISTADFVSLHMPLTPATKKVFNDETFSKMKKGVRLINVARGGVIDEDALVRALD 360

Query: 389 SGAVAQAALDVFVEEPPPKDSKLVQHKNVTVTPHLGASTKEAQEGVAIEIAEAVVGALNG 448
           +G VAQAALDVF EEPP KDS+L+QH+NVTVTPHLGASTKEAQEGVAIEIAEAV GAL G
Sbjct: 361 AGIVAQAALDVFCEEPPSKDSRLIQHENVTVTPHLGASTKEAQEGVAIEIAEAVAGALKG 420

Query: 449 ELSATAVNAPMVPPEVLSELAPYVVLAEKLGRLAVQLVAGGSGIKSVKVVYRSGRAPDDL 508
           ELSATAVNAPMV PEVLSEL PY+VLAEKLGRLAVQL +GG G++S++VVYRS R  DDL
Sbjct: 421 ELSATAVNAPMVAPEVLSELTPYIVLAEKLGRLAVQLASGGKGVQSIRVVYRSARDRDDL 480

Query: 509 DTRLLRAMITKGIIEPISDSHINLVNADFTAKQKGLRISEERVLVDAPPEFPVESIQIVV 568
           DTRLLRAMITKGIIEPISDS++NLVNADF AKQKGLRISEER++VD+ PE+PV+SIQ+ +
Sbjct: 481 DTRLLRAMITKGIIEPISDSYVNLVNADFIAKQKGLRISEERMVVDSSPEYPVDSIQVQI 540

Query: 569 SEVESKFASAVTEKGEVVIEGKVKYGVPHLRRVGSFDVDVSLEGNLILCRQVDQPGMIGQ 628
             VES FA AV++ G++ IEGKVKYGVPHL  VGSF VDVSLEGNLILCRQVDQPGMIGQ
Sbjct: 541 LNVESNFAGAVSDAGDISIEGKVKYGVPHLTCVGSFGVDVSLEGNLILCRQVDQPGMIGQ 600

Query: 629 VGNILGENNVNVNFMSVGRTLRRKRAIMAIGMDEEPNKDTLKKIGQVPAIEEFVFLNL 637
           VGNILGE NVNVNFMSVGRT+ RK+AIMAIG+DEEP+  TL++IG V AIEEFVFL L
Sbjct: 601 VGNILGEQNVNVNFMSVGRTVLRKQAIMAIGVDEEPDNKTLERIGGVSAIEEFVFLKL 651

BLAST of Cucsa.192110 vs. TAIR10
Match: AT4G34200.1 (AT4G34200.1 D-3-phosphoglycerate dehydrogenase)

HSP 1 Score: 860.1 bits (2221), Expect = 8.4e-250
Identity = 444/598 (74.25%), Postives = 518/598 (86.62%), Query Frame = 1

Query: 47  SSSSSSSSSSSSNRCSFIPLRSVLKTLESSAFVSQNSDLLD--------PTSPNDRSTIL 106
           ++SSS + +++S R   +  RS L +  S AF S+  + L          T    + TIL
Sbjct: 6   AASSSIAVATNSLRNVTLSSRSPLPSAISVAFPSRGRNTLQRRLVLVSCSTGDGSKPTIL 65

Query: 107 VAEKLGEAGLELLRSFGNVVCAYDLSPEELCAKISSCDALIVRSGTKVNRQVFEAAKGRL 166
           VAEKLG+AG++LL    NV C+Y+++PEEL  KIS CDALIVRSGTKV R+VFE++ GRL
Sbjct: 66  VAEKLGDAGIKLLEDVANVDCSYNMTPEELNIKISLCDALIVRSGTKVGREVFESSHGRL 125

Query: 167 KVVGRAGVGIDNVDLQAATEFGCLVVNAPTANTIAAAEHGIALLTSMARNVAQADASMKA 226
           KVVGRAGVGIDNVDL AATEFGCLVVNAPTANTIAAAEHGIAL+ +MARNVAQADAS+KA
Sbjct: 126 KVVGRAGVGIDNVDLSAATEFGCLVVNAPTANTIAAAEHGIALMAAMARNVAQADASVKA 185

Query: 227 GKWERNKYVGVSLVGKTLAVMGFGKVGSEVARRAKGLGMQVIAHDPYAPVDRARAIGVEL 286
           G+W+RNKYVGVSLVGKTLAV+GFGKVG+EVARRAKGLGM+VIAHDPYAP DRA AIGV+L
Sbjct: 186 GEWKRNKYVGVSLVGKTLAVLGFGKVGTEVARRAKGLGMRVIAHDPYAPADRAHAIGVDL 245

Query: 287 VSFNQAISTADFISLHMPLTPTTSKVFNDDTFGLMKKGARLINVARGGVIDEDALVRALD 346
           VSF++A++TADFISLHMPLTPTTSK+ ND+TF  MKKG R++NVARGGVIDEDALVRALD
Sbjct: 246 VSFDEALATADFISLHMPLTPTTSKILNDETFAKMKKGVRIVNVARGGVIDEDALVRALD 305

Query: 347 SGAVAQAALDVFVEEPPPKDSKLVQHKNVTVTPHLGASTKEAQEGVAIEIAEAVVGALNG 406
           +G VAQAALDVF +EPP KDSKLVQH+ VTVTPHLGAST EAQEGVAIEIAEAVVGALNG
Sbjct: 306 AGIVAQAALDVFTKEPPAKDSKLVQHERVTVTPHLGASTMEAQEGVAIEIAEAVVGALNG 365

Query: 407 ELSATAVNAPMVPPEVLSELAPYVVLAEKLGRLAVQLVAGGSGIKSVKVVYRSGRAPDDL 466
           EL+ATAVNAPMV  EVL+EL PYVVLAEKLGRLAVQLVAGGSG+K+ K+ Y S RA DDL
Sbjct: 366 ELAATAVNAPMVSAEVLTELKPYVVLAEKLGRLAVQLVAGGSGVKNAKITYASARATDDL 425

Query: 467 DTRLLRAMITKGIIEPISDSHINLVNADFTAKQKGLRISEERVLVDAPPEFPVESIQIVV 526
           DTRLLRAMITKGIIEPISD ++NLVNADFTAKQ+GLR+SEERVL+D  PE P+E+I + +
Sbjct: 426 DTRLLRAMITKGIIEPISDVYVNLVNADFTAKQRGLRLSEERVLLDGSPESPLETITVQL 485

Query: 527 SEVESKFASAVTEKGEVVIEGKVKYGVPHLRRVGSFDVDVSLEGNLILCRQVDQPGMIGQ 586
           S VESKFAS+++E GEV +EGKVK GVPHL +VGSF+VDV+LEG++ILCRQVDQPGMIG 
Sbjct: 486 SNVESKFASSLSESGEVKVEGKVKDGVPHLTKVGSFEVDVTLEGSIILCRQVDQPGMIGT 545

Query: 587 VGNILGENNVNVNFMSVGRTLRRKRAIMAIGMDEEPNKDTLKKIGQVPAIEEFVFLNL 637
           VG+ILGE+NVNVNFMSVGR   RK+AIMAIG+D+ P+K+TLKKIG++PA+EEFVFL L
Sbjct: 546 VGSILGESNVNVNFMSVGRIAPRKQAIMAIGVDDIPSKETLKKIGEIPAVEEFVFLKL 603

BLAST of Cucsa.192110 vs. TAIR10
Match: AT3G19480.1 (AT3G19480.1 D-3-phosphoglycerate dehydrogenase)

HSP 1 Score: 820.5 bits (2118), Expect = 7.4e-238
Identity = 423/587 (72.06%), Postives = 495/587 (84.33%), Query Frame = 1

Query: 55  SSSSNRCSFIPLRSVLKTLESSAF-VSQNSDLLDPTSPND----RSTILVAEKLGEAGLE 114
           ++S N  S     S L T  SS F + Q   ++  TS +     + TILV EKLG+AG++
Sbjct: 2   ATSLNLSSIFSSSSRLVTTPSSVFPIRQRRRIILVTSSSSGGGGKPTILVTEKLGQAGID 61

Query: 115 LLRSFGNVVCAYDLSPEELCAKISSCDALIVRSGTKVNRQVFEAAKGRLKVVGRAGVGID 174
           LL+ + NV C+YDLS EELC KIS CDALIVRSGTKV R VFE+++GRLKVVGRAGVGID
Sbjct: 62  LLKKYANVDCSYDLSLEELCTKISLCDALIVRSGTKVGRDVFESSRGRLKVVGRAGVGID 121

Query: 175 NVDLQAATEFGCLVVNAPTANTIAAAEHGIALLTSMARNVAQADASMKAGKWERNKYVGV 234
           NVDL AATE+GCLVVNAPTANT+AAAEHGIALLT+MARN+AQADAS+KAGKW RNKYVGV
Sbjct: 122 NVDLAAATEYGCLVVNAPTANTVAAAEHGIALLTAMARNIAQADASIKAGKWTRNKYVGV 181

Query: 235 SLVGKTLAVMGFGKVGSEVARRAKGLGMQVIAHDPYAPVDRARAIGVELVSFNQAISTAD 294
           SLVGKTLAV+GFGKVGSEVARRA+GLGM VI HDPYAP DRARAIGVELVSF  AISTAD
Sbjct: 182 SLVGKTLAVLGFGKVGSEVARRARGLGMHVITHDPYAPADRARAIGVELVSFEVAISTAD 241

Query: 295 FISLHMPLTPTTSKVFNDDTFGLMKKGARLINVARGGVIDEDALVRALDSGAVAQAALDV 354
           FISLH+PLT  TSK+ ND TF +MKKG R++NVARGGVIDE+AL+RALDSG VAQAALDV
Sbjct: 242 FISLHLPLTAATSKMMNDVTFAMMKKGVRIVNVARGGVIDEEALLRALDSGIVAQAALDV 301

Query: 355 FVEEPPPKDSKLVQHKNVTVTPHLGASTKEAQEGVAIEIAEAVVGALNGELSATAVNAPM 414
           F  EPP KD+KLV H++VT TPHLGAST EAQEGV+IE+AEAV+GAL GEL+ATAVNAPM
Sbjct: 302 FTVEPPVKDNKLVLHESVTATPHLGASTMEAQEGVSIEVAEAVIGALRGELAATAVNAPM 361

Query: 415 VPPEVLSELAPYVVLAEKLGRLAVQLVAGGSGIKSVKVVYRSGRAPDDLDTRLLRAMITK 474
           VP EVL EL PYVVLAEKLGRLAVQLV GGSG+ +VKV Y S RAPDDLDTRLLRAM+ K
Sbjct: 362 VPLEVLRELKPYVVLAEKLGRLAVQLVTGGSGVNAVKVTYASSRAPDDLDTRLLRAMVIK 421

Query: 475 GIIEPISDSHINLVNADFTAKQKGLRISEERVLVDAPPEFPVESIQIVVSEVESKFASAV 534
           GIIEPIS   INLVN+D+ AKQ+G++ISEER+++D  PE P+E I + ++ VES+FASA+
Sbjct: 422 GIIEPISSVFINLVNSDYIAKQRGVKISEERMVLDGSPENPIEYITVRIANVESRFASAL 481

Query: 535 TEKGEVVIEGKVKYGVPHLRRVGSFDVDVSLEGNLILCRQVDQPGMIGQVGNILGENNVN 594
           +E GE+ +EG+VK GVP L +VG F VDVSLEG++ILCRQVDQPGMIG+V +ILG+ NVN
Sbjct: 482 SESGEIKVEGRVKQGVPSLTKVGLFGVDVSLEGSVILCRQVDQPGMIGKVASILGDENVN 541

Query: 595 VNFMSVGRTLRRKRAIMAIGMDEEPNKDTLKKIGQVPAIEEFVFLNL 637
           V+FMSVGR    K+A+MAIG+DE+P+K+TLKKIG +PAIEEFVFL L
Sbjct: 542 VSFMSVGRIAPGKQAVMAIGVDEQPSKETLKKIGDIPAIEEFVFLKL 588

BLAST of Cucsa.192110 vs. TAIR10
Match: AT1G68010.2 (AT1G68010.2 hydroxypyruvate reductase)

HSP 1 Score: 129.4 bits (324), Expect = 7.8e-30
Identity = 104/299 (34.78%), Postives = 149/299 (49.83%), Query Frame = 1

Query: 123 LSPEELCAKISS-CDALIVRSGTKVNRQVFEA-AKGRLKVVGRAGVGIDNVDLQAATEFG 182
           LS E++   I   CD +I +        +F A +K   K      VG +NVD++AA ++G
Sbjct: 50  LSVEDIIDLIGDKCDGVIGQLTEDWGETLFSALSKAGGKAFSNMAVGYNNVDVEAANKYG 109

Query: 183 CLVVNAPTANTIAAAEHGIALLTSMARNVAQADASMKAG---KWERNKYVGVSLVGKTLA 242
             V N P   T   AE   +L  + AR + +AD  M+ G    W  + +VG  L G+T+ 
Sbjct: 110 IAVGNTPGVLTETTAELAASLSLAAARRIVEADEFMRGGLYEGWLPHLFVGNLLKGQTVG 169

Query: 243 VMGFGKVGSEVAR-RAKGLGMQVIAHDPY----------APVDRARAIGVELVSFNQAIS 302
           V+G G++GS  AR   +G  M +I  D Y          A     +A G + V++ +A S
Sbjct: 170 VIGAGRIGSAYARMMVEGFKMNLIYFDLYQSTRLEKFVTAYGQFLKANGEQPVTWKRASS 229

Query: 303 ------TADFISLHMPLTPTTSKVFNDDTFGLMKK-GARLINVARGGVIDEDALVRALDS 362
                  AD ISLH  L  TT  + N +   +MKK  A L+N +RG VIDE ALV  L  
Sbjct: 230 MEEVLREADLISLHPVLDKTTYHLVNKERLAMMKKVEAILVNCSRGPVIDEAALVEHLKE 289

Query: 363 GAVAQAALDVFVEEPPPKDSKLVQHKNVTVTPHLGASTKEAQEGVAIEIAEAVVGALNG 399
             + +  LDVF EEP  K   L   KN  V PH+ +++K  +EG+A   A  V+G + G
Sbjct: 290 NPMFRVGLDVFEEEPFMKPG-LADTKNAIVVPHIASASKWTREGMATLAALNVLGRVKG 347

BLAST of Cucsa.192110 vs. TAIR10
Match: AT5G14780.1 (AT5G14780.1 formate dehydrogenase)

HSP 1 Score: 122.1 bits (305), Expect = 1.2e-27
Identity = 73/229 (31.88%), Postives = 119/229 (51.97%), Query Frame = 1

Query: 158 LKVVGRAGVGIDNVDLQAATEFGCLVVNAPTANTIAAAEHGIALLTSMARNVAQADASMK 217
           LK++  AG+G D++DLQAA   G  V     +N ++ AE  +  +  + RN       + 
Sbjct: 120 LKLLLTAGIGSDHIDLQAAAAAGLTVAEVTGSNVVSVAEDELMRILILMRNFVPGYNQVV 179

Query: 218 AGKWERN--KYVGVSLVGKTLAVMGFGKVGSEVARRAKGLGMQVIAHD--PYAPVDRARA 277
            G+W      Y    L GKT+  +G G++G  + +R K  G  ++ HD    AP +  + 
Sbjct: 180 KGEWNVAGIAYRAYDLEGKTIGTVGAGRIGKLLLQRLKPFGCNLLYHDRLQMAP-ELEKE 239

Query: 278 IGVELVS-FNQAISTADFISLHMPLTPTTSKVFNDDTFGLMKKGARLINVARGGVIDEDA 337
            G + V   N+ +   D I ++MPLT  T  +FN +  G +KKG  ++N ARG +++  A
Sbjct: 240 TGAKFVEDLNEMLPKCDVIVINMPLTEKTRGMFNKELIGKLKKGVLIVNNARGAIMERQA 299

Query: 338 LVRALDSGAVAQAALDVFVEEPPPKDSKLVQHKNVTVTPHLGASTKEAQ 382
           +V A++SG +   + DV+  +P PKD       N  +TPH   +T +AQ
Sbjct: 300 VVDAVESGHIGGYSGDVWDPQPAPKDHPWRYMPNQAMTPHTSGTTIDAQ 347

BLAST of Cucsa.192110 vs. NCBI nr
Match: gi|778682562|ref|XP_004148563.2| (PREDICTED: D-3-phosphoglycerate dehydrogenase 1, chloroplastic [Cucumis sativus])

HSP 1 Score: 1205.7 bits (3118), Expect = 0.0e+00
Identity = 632/636 (99.37%), Postives = 632/636 (99.37%), Query Frame = 1

Query: 1   MASSSSNSLFSSPHFTSFNRSFHSKPPLLSFSTSSVSLNPRPLHSFSSSSSSSSSSSSNR 60
           MASSSSNSLFSSPHFTSFNRSFHSKPPLLSFSTSSVSLNPRPLHS    SSSSSSSSSNR
Sbjct: 1   MASSSSNSLFSSPHFTSFNRSFHSKPPLLSFSTSSVSLNPRPLHS----SSSSSSSSSNR 60

Query: 61  CSFIPLRSVLKTLESSAFVSQNSDLLDPTSPNDRSTILVAEKLGEAGLELLRSFGNVVCA 120
           CSFIPLRSVLKTLESSAFVSQNSDLLDPTSPNDRSTILVAEKLGEAGLELLRSFGNVVCA
Sbjct: 61  CSFIPLRSVLKTLESSAFVSQNSDLLDPTSPNDRSTILVAEKLGEAGLELLRSFGNVVCA 120

Query: 121 YDLSPEELCAKISSCDALIVRSGTKVNRQVFEAAKGRLKVVGRAGVGIDNVDLQAATEFG 180
           YDLSPEELCAKISSCDALIVRSGTKVNRQVFEAAKGRLKVVGRAGVGIDNVDLQAATEFG
Sbjct: 121 YDLSPEELCAKISSCDALIVRSGTKVNRQVFEAAKGRLKVVGRAGVGIDNVDLQAATEFG 180

Query: 181 CLVVNAPTANTIAAAEHGIALLTSMARNVAQADASMKAGKWERNKYVGVSLVGKTLAVMG 240
           CLVVNAPTANTIAAAEHGIALLTSMARNVAQADASMKAGKWERNKYVGVSLVGKTLAVMG
Sbjct: 181 CLVVNAPTANTIAAAEHGIALLTSMARNVAQADASMKAGKWERNKYVGVSLVGKTLAVMG 240

Query: 241 FGKVGSEVARRAKGLGMQVIAHDPYAPVDRARAIGVELVSFNQAISTADFISLHMPLTPT 300
           FGKVGSEVARRAKGLGMQVIAHDPYAPVDRARAIGVELVSFNQAISTADFISLHMPLTPT
Sbjct: 241 FGKVGSEVARRAKGLGMQVIAHDPYAPVDRARAIGVELVSFNQAISTADFISLHMPLTPT 300

Query: 301 TSKVFNDDTFGLMKKGARLINVARGGVIDEDALVRALDSGAVAQAALDVFVEEPPPKDSK 360
           TSKVFNDDTFGLMKKGARLINVARGGVIDEDALVRALDSGAVAQAALDVFVEEPPPKDSK
Sbjct: 301 TSKVFNDDTFGLMKKGARLINVARGGVIDEDALVRALDSGAVAQAALDVFVEEPPPKDSK 360

Query: 361 LVQHKNVTVTPHLGASTKEAQEGVAIEIAEAVVGALNGELSATAVNAPMVPPEVLSELAP 420
           LVQHKNVTVTPHLGASTKEAQEGVAIEIAEAVVGALNGELSATAVNAPMVPPEVLSELAP
Sbjct: 361 LVQHKNVTVTPHLGASTKEAQEGVAIEIAEAVVGALNGELSATAVNAPMVPPEVLSELAP 420

Query: 421 YVVLAEKLGRLAVQLVAGGSGIKSVKVVYRSGRAPDDLDTRLLRAMITKGIIEPISDSHI 480
           YVVLAEKLGRLAVQLVAGGSGIKSVKVVYRSGRAPDDLDTRLLRAMITKGIIEPISDSHI
Sbjct: 421 YVVLAEKLGRLAVQLVAGGSGIKSVKVVYRSGRAPDDLDTRLLRAMITKGIIEPISDSHI 480

Query: 481 NLVNADFTAKQKGLRISEERVLVDAPPEFPVESIQIVVSEVESKFASAVTEKGEVVIEGK 540
           NLVNADFTAKQKGLRISEERVLVDAPPEFPVESIQIVVSEVESKFASAVTEKGEVVIEGK
Sbjct: 481 NLVNADFTAKQKGLRISEERVLVDAPPEFPVESIQIVVSEVESKFASAVTEKGEVVIEGK 540

Query: 541 VKYGVPHLRRVGSFDVDVSLEGNLILCRQVDQPGMIGQVGNILGENNVNVNFMSVGRTLR 600
           VKYGVPHLRRVGSFDVDVSLEGNLILCRQVDQPGMIGQVGNILGENNVNVNFMSVGRTLR
Sbjct: 541 VKYGVPHLRRVGSFDVDVSLEGNLILCRQVDQPGMIGQVGNILGENNVNVNFMSVGRTLR 600

Query: 601 RKRAIMAIGMDEEPNKDTLKKIGQVPAIEEFVFLNL 637
           RKRAIMAIGMDEEPNKDTLKKIGQVPAIEEFVFLNL
Sbjct: 601 RKRAIMAIGMDEEPNKDTLKKIGQVPAIEEFVFLNL 632

BLAST of Cucsa.192110 vs. NCBI nr
Match: gi|659127975|ref|XP_008463985.1| (PREDICTED: D-3-phosphoglycerate dehydrogenase, chloroplastic-like [Cucumis melo])

HSP 1 Score: 1171.0 bits (3028), Expect = 0.0e+00
Identity = 612/636 (96.23%), Postives = 624/636 (98.11%), Query Frame = 1

Query: 1   MASSSSNSLFSSPHFTSFNRSFHSKPPLLSFSTSSVSLNPRPLHSFSSSSSSSSSSSSNR 60
           MASSSS SLFSSPHFTSFNRS HSK PLLSFSTSSVSLNPRPLHS    S +SSSSSSNR
Sbjct: 1   MASSSSKSLFSSPHFTSFNRSSHSKSPLLSFSTSSVSLNPRPLHS----SITSSSSSSNR 60

Query: 61  CSFIPLRSVLKTLESSAFVSQNSDLLDPTSPNDRSTILVAEKLGEAGLELLRSFGNVVCA 120
           CSFIPLRSVLKTLESSAFVSQNSDL+DPTSPNDRSTILVAEKLGEAGLELLRSFGNVVCA
Sbjct: 61  CSFIPLRSVLKTLESSAFVSQNSDLVDPTSPNDRSTILVAEKLGEAGLELLRSFGNVVCA 120

Query: 121 YDLSPEELCAKISSCDALIVRSGTKVNRQVFEAAKGRLKVVGRAGVGIDNVDLQAATEFG 180
           YDLSPEELCAKISSCDALIVRSGTKVNRQVFEAAKGRLKVVGRAGVGIDNVDLQAATEFG
Sbjct: 121 YDLSPEELCAKISSCDALIVRSGTKVNRQVFEAAKGRLKVVGRAGVGIDNVDLQAATEFG 180

Query: 181 CLVVNAPTANTIAAAEHGIALLTSMARNVAQADASMKAGKWERNKYVGVSLVGKTLAVMG 240
           CLVVNAPTANTIAAAEHGIALLT+MARNVAQADAS+KAGKWERNKYVGVSLVGKTLAVMG
Sbjct: 181 CLVVNAPTANTIAAAEHGIALLTAMARNVAQADASIKAGKWERNKYVGVSLVGKTLAVMG 240

Query: 241 FGKVGSEVARRAKGLGMQVIAHDPYAPVDRARAIGVELVSFNQAISTADFISLHMPLTPT 300
           FGKVGSEVARRAKGLGMQVIAHDPYAPVDRARAIGVELVSFNQAISTADFISLHMPLT  
Sbjct: 241 FGKVGSEVARRAKGLGMQVIAHDPYAPVDRARAIGVELVSFNQAISTADFISLHMPLTSA 300

Query: 301 TSKVFNDDTFGLMKKGARLINVARGGVIDEDALVRALDSGAVAQAALDVFVEEPPPKDSK 360
           TSKVFNDDTFGLMKKGARLINVARGGVIDEDALVRALD+GAVAQAALDVFVEEPPPKDSK
Sbjct: 301 TSKVFNDDTFGLMKKGARLINVARGGVIDEDALVRALDTGAVAQAALDVFVEEPPPKDSK 360

Query: 361 LVQHKNVTVTPHLGASTKEAQEGVAIEIAEAVVGALNGELSATAVNAPMVPPEVLSELAP 420
           LVQHKNVTVTPHLGASTKEAQEGVAIEIAEAVVGALNGELSATAVNAPMV PEVLSELAP
Sbjct: 361 LVQHKNVTVTPHLGASTKEAQEGVAIEIAEAVVGALNGELSATAVNAPMVAPEVLSELAP 420

Query: 421 YVVLAEKLGRLAVQLVAGGSGIKSVKVVYRSGRAPDDLDTRLLRAMITKGIIEPISDSHI 480
           YVVLAEKLGRLAVQLVAGGSGIKSVKVVY+SGRAPDDLDTRLLRAMITKGIIEPISDSHI
Sbjct: 421 YVVLAEKLGRLAVQLVAGGSGIKSVKVVYKSGRAPDDLDTRLLRAMITKGIIEPISDSHI 480

Query: 481 NLVNADFTAKQKGLRISEERVLVDAPPEFPVESIQIVVSEVESKFASAVTEKGEVVIEGK 540
           NLVNADFTAKQKGLRISEERVLVDAPPEFPVESIQ+VVSEVESKFASAVTEKGEV IEGK
Sbjct: 481 NLVNADFTAKQKGLRISEERVLVDAPPEFPVESIQVVVSEVESKFASAVTEKGEVAIEGK 540

Query: 541 VKYGVPHLRRVGSFDVDVSLEGNLILCRQVDQPGMIGQVGNILGENNVNVNFMSVGRTLR 600
           VKYGVPHLRRVGSFDVDVSLEGNLILCRQVDQPGMIG+VGNILGENNVNVNFMSVGRT+R
Sbjct: 541 VKYGVPHLRRVGSFDVDVSLEGNLILCRQVDQPGMIGRVGNILGENNVNVNFMSVGRTMR 600

Query: 601 RKRAIMAIGMDEEPNKDTLKKIGQVPAIEEFVFLNL 637
           RKRAIMAIG+DEEPNKDTL++IGQVPAIEEFVFLNL
Sbjct: 601 RKRAIMAIGVDEEPNKDTLRQIGQVPAIEEFVFLNL 632

BLAST of Cucsa.192110 vs. NCBI nr
Match: gi|698582849|ref|XP_009777942.1| (PREDICTED: D-3-phosphoglycerate dehydrogenase 2, chloroplastic [Nicotiana sylvestris])

HSP 1 Score: 953.4 bits (2463), Expect = 2.1e-274
Identity = 508/636 (79.87%), Postives = 560/636 (88.05%), Query Frame = 1

Query: 2   ASSSSNSLFSSPHFTSFNRSFHSKPPLLSF-STSSVSLNPRPLHSFSSSSSSSSSSSSNR 61
           A++  NSL SS   +S N   H+KP  LSF   SS S + +  HS S+S S S+SSS+  
Sbjct: 11  ATTLQNSLLSSSS-SSVN---HNKPSNLSFLHASSNSSSLKLFHSVSASFSPSNSSST-- 70

Query: 62  CSFIPLRSVLKTLESSAFVSQNSDLLDPTSPNDRSTILVAEKLGEAGLELLRSFGNVVCA 121
                + +V+KT+ES+     N+  LD      + TILV+EKLGEAGL+LLRSFGNV C+
Sbjct: 71  -----ICNVIKTVESADISFSNARDLDGVVSIPKPTILVSEKLGEAGLDLLRSFGNVDCS 130

Query: 122 YDLSPEELCAKISSCDALIVRSGTKVNRQVFEAAKGRLKVVGRAGVGIDNVDLQAATEFG 181
           YDLSP++LCAKIS CDALIVRSGTKV R+VFEAA+GRLKVVGRAGVGIDNVDLQAATEFG
Sbjct: 131 YDLSPQDLCAKISLCDALIVRSGTKVTREVFEAAQGRLKVVGRAGVGIDNVDLQAATEFG 190

Query: 182 CLVVNAPTANTIAAAEHGIALLTSMARNVAQADASMKAGKWERNKYVGVSLVGKTLAVMG 241
           CLVVNAPTANTIAAAEHGIALLTSMARNVAQADASMKAGKW R+KYVGVSLVGKTLAVMG
Sbjct: 191 CLVVNAPTANTIAAAEHGIALLTSMARNVAQADASMKAGKWLRSKYVGVSLVGKTLAVMG 250

Query: 242 FGKVGSEVARRAKGLGMQVIAHDPYAPVDRARAIGVELVSFNQAISTADFISLHMPLTPT 301
           FGKVGSEVARRAKGLG+ VIAHDPYAP DRARAIGV+LVSF QAI+TADF+SLHMPLTP 
Sbjct: 251 FGKVGSEVARRAKGLGIHVIAHDPYAPADRARAIGVDLVSFEQAIATADFVSLHMPLTPA 310

Query: 302 TSKVFNDDTFGLMKKGARLINVARGGVIDEDALVRALDSGAVAQAALDVFVEEPPPKDSK 361
           T KVFNDDTF  MKKG RLINVARGGVIDEDALVRALDSG VAQAALDVF  EPPPKDSK
Sbjct: 311 TKKVFNDDTFAKMKKGVRLINVARGGVIDEDALVRALDSGIVAQAALDVFTVEPPPKDSK 370

Query: 362 LVQHKNVTVTPHLGASTKEAQEGVAIEIAEAVVGALNGELSATAVNAPMVPPEVLSELAP 421
           LVQH+NVTVTPHLGASTKEAQEGVAIEIAEAVVGALNGELSATAVNAPMVPPEVLSELAP
Sbjct: 371 LVQHENVTVTPHLGASTKEAQEGVAIEIAEAVVGALNGELSATAVNAPMVPPEVLSELAP 430

Query: 422 YVVLAEKLGRLAVQLVAGGSGIKSVKVVYRSGRAPDDLDTRLLRAMITKGIIEPISDSHI 481
           YVVLAEKLGRLAVQLV GGSGI++VKVVY+S R PD LDTRLLRAM+TKGIIEPISD+ I
Sbjct: 431 YVVLAEKLGRLAVQLVTGGSGIQNVKVVYKSARDPDSLDTRLLRAMVTKGIIEPISDTII 490

Query: 482 NLVNADFTAKQKGLRISEERVLVDAPPEFPVESIQIVVSEVESKFASAVTEKGEVVIEGK 541
           NLVNADF+AKQKGLRISEERV+VD+ PE+PVESIQ+ +S V+S+FASA++E G + IEGK
Sbjct: 491 NLVNADFSAKQKGLRISEERVIVDSSPEYPVESIQVQISNVQSRFASALSENGNISIEGK 550

Query: 542 VKYGVPHLRRVGSFDVDVSLEGNLILCRQVDQPGMIGQVGNILGENNVNVNFMSVGRTLR 601
           VKYGVPHL RVGSF VDVSLEGNLILC+QVDQPGMIGQVGNILGE+NVNV+FMSVGRT+R
Sbjct: 551 VKYGVPHLTRVGSFSVDVSLEGNLILCKQVDQPGMIGQVGNILGESNVNVSFMSVGRTVR 610

Query: 602 RKRAIMAIGMDEEPNKDTLKKIGQVPAIEEFVFLNL 637
           RK+AIMAIG+DEEPNKDT KKIG+V AIEEFVFL L
Sbjct: 611 RKQAIMAIGVDEEPNKDTQKKIGEVSAIEEFVFLKL 635

BLAST of Cucsa.192110 vs. NCBI nr
Match: gi|697114181|ref|XP_009610991.1| (PREDICTED: D-3-phosphoglycerate dehydrogenase 2, chloroplastic [Nicotiana tomentosiformis])

HSP 1 Score: 951.8 bits (2459), Expect = 6.0e-274
Identity = 507/636 (79.72%), Postives = 560/636 (88.05%), Query Frame = 1

Query: 2   ASSSSNSLFSSPHFTSFNRSFHSKPPLLSF-STSSVSLNPRPLHSFSSSSSSSSSSSSNR 61
           A++  NSL SS   +S N   H+KP  LSF   SS S + + +HS S+S S S+SSS+  
Sbjct: 11  ATTLQNSLLSSSS-SSIN---HNKPSNLSFLHASSNSSSLKLVHSVSASFSPSNSSST-- 70

Query: 62  CSFIPLRSVLKTLESSAFVSQNSDLLDPTSPNDRSTILVAEKLGEAGLELLRSFGNVVCA 121
                + +VLKT+ES+     N+  LD      +  ILV+EKLGEAGL+LLRSFGNV C+
Sbjct: 71  -----ICNVLKTVESADISFSNARDLDGVVSIPKPIILVSEKLGEAGLDLLRSFGNVDCS 130

Query: 122 YDLSPEELCAKISSCDALIVRSGTKVNRQVFEAAKGRLKVVGRAGVGIDNVDLQAATEFG 181
           YDLSP++LCAKIS CDALIVRSGTKV RQVFEAA+GRLKVVGRAGVGIDNVDLQAATEFG
Sbjct: 131 YDLSPQDLCAKISLCDALIVRSGTKVTRQVFEAAQGRLKVVGRAGVGIDNVDLQAATEFG 190

Query: 182 CLVVNAPTANTIAAAEHGIALLTSMARNVAQADASMKAGKWERNKYVGVSLVGKTLAVMG 241
           CLVVNAPTANTIAAAEHGIALLTSMARNVAQADASMKAGKW R+KYVGVSLVGKTLAVMG
Sbjct: 191 CLVVNAPTANTIAAAEHGIALLTSMARNVAQADASMKAGKWLRSKYVGVSLVGKTLAVMG 250

Query: 242 FGKVGSEVARRAKGLGMQVIAHDPYAPVDRARAIGVELVSFNQAISTADFISLHMPLTPT 301
           FGKVGSEVARRAKGLGM VIAHDPYAP DRARAIGV+LVSF QAI+TADF+SLHMPLTP 
Sbjct: 251 FGKVGSEVARRAKGLGMHVIAHDPYAPADRARAIGVDLVSFEQAIATADFVSLHMPLTPA 310

Query: 302 TSKVFNDDTFGLMKKGARLINVARGGVIDEDALVRALDSGAVAQAALDVFVEEPPPKDSK 361
           T KVFNDDTF  MKKG RLINVARGGVIDEDALVRALDSG VAQAALDVF  EPPPKDSK
Sbjct: 311 TKKVFNDDTFAKMKKGVRLINVARGGVIDEDALVRALDSGIVAQAALDVFTVEPPPKDSK 370

Query: 362 LVQHKNVTVTPHLGASTKEAQEGVAIEIAEAVVGALNGELSATAVNAPMVPPEVLSELAP 421
           LVQH+NVTVTPHLGASTKEAQEGVAIEIAEAVVGALNGELSATAVNAPMVPPEVLSELAP
Sbjct: 371 LVQHENVTVTPHLGASTKEAQEGVAIEIAEAVVGALNGELSATAVNAPMVPPEVLSELAP 430

Query: 422 YVVLAEKLGRLAVQLVAGGSGIKSVKVVYRSGRAPDDLDTRLLRAMITKGIIEPISDSHI 481
           YVVLAEKLGRLAVQLV GGSGI++VKVVY+S R PD LDTRLLRAM+TKGIIEPISD+ I
Sbjct: 431 YVVLAEKLGRLAVQLVTGGSGIQNVKVVYKSARDPDSLDTRLLRAMVTKGIIEPISDTII 490

Query: 482 NLVNADFTAKQKGLRISEERVLVDAPPEFPVESIQIVVSEVESKFASAVTEKGEVVIEGK 541
           NLVNADF+AKQKGLRISEERV+VD+ PE+PVE+IQ+ +S V+S+FASA++E G + IEGK
Sbjct: 491 NLVNADFSAKQKGLRISEERVIVDSSPEYPVETIQVQISNVQSRFASALSENGNISIEGK 550

Query: 542 VKYGVPHLRRVGSFDVDVSLEGNLILCRQVDQPGMIGQVGNILGENNVNVNFMSVGRTLR 601
           VKYGVPHL RVGSF VDVSLEGNLILC+QVDQPGMIG+VGNILGE+NVNV+FMSVGRT++
Sbjct: 551 VKYGVPHLTRVGSFSVDVSLEGNLILCKQVDQPGMIGEVGNILGESNVNVSFMSVGRTVK 610

Query: 602 RKRAIMAIGMDEEPNKDTLKKIGQVPAIEEFVFLNL 637
           RK+AIMAIG+DEEPNKDT KKIG+V AIEEFVFL L
Sbjct: 611 RKQAIMAIGVDEEPNKDTQKKIGEVSAIEEFVFLKL 635

BLAST of Cucsa.192110 vs. NCBI nr
Match: gi|970017524|ref|XP_015069902.1| (PREDICTED: D-3-phosphoglycerate dehydrogenase 3, chloroplastic-like [Solanum pennellii])

HSP 1 Score: 946.0 bits (2444), Expect = 3.3e-272
Identity = 504/631 (79.87%), Postives = 559/631 (88.59%), Query Frame = 1

Query: 7   NSLFSSPHFTSFNRSFHSKPPLLSF-STSSVSLNPRPLHSFSSSSSSSSSSSSNRCSFIP 66
           NSL SS   +SF    H+KP  LSF   SS   + + LHS S S SSSSSS++       
Sbjct: 13  NSLLSSSSSSSFVN--HNKPSNLSFLHASSNPSSIKLLHSVSPSISSSSSSTT------A 72

Query: 67  LRSVLKTLESSAFVSQNSDLLDPTSPNDRSTILVAEKLGEAGLELLRSFGNVVCAYDLSP 126
           +R+VLKT+ES A +S + DL    S + + TILV+EKLGEAGL+LL+SFGNV C+YDLSP
Sbjct: 73  IRNVLKTVES-ADISLSRDLHGVVSTS-KPTILVSEKLGEAGLDLLKSFGNVDCSYDLSP 132

Query: 127 EELCAKISSCDALIVRSGTKVNRQVFEAAKGRLKVVGRAGVGIDNVDLQAATEFGCLVVN 186
           ++LCAKIS CDALIVRSGTKV R VFEAA+GRLKVVGRAGVGIDNVDLQAATEFGCLVVN
Sbjct: 133 QDLCAKISLCDALIVRSGTKVTRDVFEAAQGRLKVVGRAGVGIDNVDLQAATEFGCLVVN 192

Query: 187 APTANTIAAAEHGIALLTSMARNVAQADASMKAGKWERNKYVGVSLVGKTLAVMGFGKVG 246
           APTANTIAAAEHGIALLTSMARNVAQ+DASMKAGKW R+KYVGVSLVGKTLA+MGFGKVG
Sbjct: 193 APTANTIAAAEHGIALLTSMARNVAQSDASMKAGKWLRSKYVGVSLVGKTLAIMGFGKVG 252

Query: 247 SEVARRAKGLGMQVIAHDPYAPVDRARAIGVELVSFNQAISTADFISLHMPLTPTTSKVF 306
           SEVARRAKGLGM VIAHDPYAP DRARAIGV+LVSF QAISTADFISLHMPLTP T+KVF
Sbjct: 253 SEVARRAKGLGMHVIAHDPYAPADRARAIGVDLVSFEQAISTADFISLHMPLTPATNKVF 312

Query: 307 NDDTFGLMKKGARLINVARGGVIDEDALVRALDSGAVAQAALDVFVEEPPPKDSKLVQHK 366
           NDDTF  MKKG RLINVARGGVIDEDALVRALDSG VAQAALDVF  EPPPKDSKLVQH+
Sbjct: 313 NDDTFAKMKKGVRLINVARGGVIDEDALVRALDSGIVAQAALDVFTVEPPPKDSKLVQHE 372

Query: 367 NVTVTPHLGASTKEAQEGVAIEIAEAVVGALNGELSATAVNAPMVPPEVLSELAPYVVLA 426
           NVTVTPHLGASTKEAQEGVAIEIAEAVVGALNGELSATAVNAPMVPPEVLSELAPYVVLA
Sbjct: 373 NVTVTPHLGASTKEAQEGVAIEIAEAVVGALNGELSATAVNAPMVPPEVLSELAPYVVLA 432

Query: 427 EKLGRLAVQLVAGGSGIKSVKVVYRSGRAPDDLDTRLLRAMITKGIIEPISDSHINLVNA 486
           EK+GRLAVQLV GGSGI+SVKVVY+S R PD LDTRLLRAM+TKGIIEPISD+ INLVNA
Sbjct: 433 EKVGRLAVQLVTGGSGIQSVKVVYKSARDPDSLDTRLLRAMVTKGIIEPISDTIINLVNA 492

Query: 487 DFTAKQKGLRISEERVLVDAPPEFPVESIQIVVSEVESKFASAVTEKGEVVIEGKVKYGV 546
           DF+AKQKGLRISEER++VD+ PE+PVESIQ+ +S V+S+FASA++E G + IEG+VKYGV
Sbjct: 493 DFSAKQKGLRISEERIIVDSSPEYPVESIQVQISNVQSRFASALSENGNISIEGRVKYGV 552

Query: 547 PHLRRVGSFDVDVSLEGNLILCRQVDQPGMIGQVGNILGENNVNVNFMSVGRTLRRKRAI 606
           PHL RVG F VDVSLEGNLILC+QVDQPGMIG+VGNILGE+NVNV+FMSVGRT++ K+AI
Sbjct: 553 PHLTRVGPFSVDVSLEGNLILCKQVDQPGMIGKVGNILGESNVNVSFMSVGRTVKGKQAI 612

Query: 607 MAIGMDEEPNKDTLKKIGQVPAIEEFVFLNL 637
           MAIG+DEEP+KDT KKIG+V A+EEFVFL L
Sbjct: 613 MAIGVDEEPDKDTQKKIGEVSAVEEFVFLKL 633