Cucsa.184640 (gene) Cucumber (Gy14) v1

NameCucsa.184640
Typegene
OrganismCucumis sativus (Cucumber (Gy14) v1)
DescriptiontRNA-2-methylthio-N(6)-dimethylallyladenosine synthase
Locationscaffold01274 : 291488 .. 294517 (+)
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGACCAGATCCTTACCAGCTTCTGACGTGCCTTTAAGCGGTCGTGTTTATCATGAAACCTATGGATGTCAAATGAATATTAATGATATGGAGGTAGTTCTATCTATCATGAAAAAAGCTGGATACAGTGAAACTGTTGACACTCCTGAGACTGCAGAGATCATTTTTATCAACACTTGTGCTATCAGGGACAATGCTGAACAGAAGGTGTGGCAGAGACTTAATTACTTTTGGTTTCTGAAGAGAAATTGGAAAAGTAATGTTGCTACCGGAAGATCACAGTCTCGACATCCTCCAAAAGTAGTTGTTTTGGGGTGTATGGCTGAGAGGTTGAAAGATAAAATACTAGATTCAGACAAGATGGTTGATGTGGTTTGCGGACCTGATGCTTACAGAGATCTACCAAGATTATTGGATGAAGTAGATTATGGTCAGAAGGGAATAAACACACTTCTTTCCCTTGAAGAAACTTATGCTGATATAAGTCCTGTACGAATAGCCAAAAATTCAGTCACTGCATTTGTATCTGTGATGAGAGGTTGCAACAATATGTGCTCATTCTGCATTGTTCCTTTTACCAGGGGCAGAGAGCGGTCACGTCCTGTTGAATCAATTGTGTGTGAGGTTAGAGAACTTTATAGAGAAGGTGTGAAAGAGGTAACTCTTCTTGGTCAGAACGTTAACAGCTATAACGATACCACTGAGAATGCAGACATAGAACCAGGAACTAATTGGAAGCTCAGTGATGGATTTTCCACCATAGCCAAGGTTAAGAAAATGGGTTtGCGTTTTTCCGATCTCTTAGATCGTCTATCCACTGAATTTCCTGAGATGAGGTTCAGATACACATCTCCACATCCTAAAGACTTTCCCGATGAATTCCTTTATTTAATGCGAGACAGACACAACATTTGCAAAAGCATCCATTTACCTGCTCAGAGTGGGAATAGCATAGTTCTTGAAAGAATGCGTAGGGGATACACCCGAGAGGCCTACTTGGATCTTGTGCACAAGATTCGATCAATCATTCCAGATGTGGGAATAACTAGTGATTTCATTTGTGGTATGTGGGCCTTTTTAACTTCTATAATATTTTCTTATTTAAAAAAATCTGCAATATTCTGGATAAATATTGATTATTTTGTATGAAGCATTGGTACCCATACCTCGAGTGGTTAGAAGTGCCTAGCCAATGGCAGTGGTTATTGGATCAGGATGTAGAACTTCTTTGTTTTTAAAATCTCTTTACTTTCTTTAGTCAACCATTTTTTtAAATTTTTTTGAAAAGGGATGGAACTTTTCATTGATAAAATGAGAATAGACTAATGCTCAAAGATACAATAATCCGTAACCATTTTAGCTGTACTGTAATAATGTCTGCATCAGAAATCAGATTGGAAATATGTAAAACAATCTAAGCTTCAATTGAAACACTTGCTGATTCATTTGTCAGTTAACTGGCTAATGTTTGTTCTTTCATTTCAATTTCTCTCATCGTCATCTTACAGTACTTGTAAAAACACTAATCGTTCTACACTTCTGCTGCTGGAGGACCACATTTGTGAATCTGTCATTGCTTCTCTTATAAATTGGATTCGACTGTCGTTTTTATCCCTGTAATATTGACCCTCTTTATTTAAATCATCGAACTTTTAAAGTATAATTCAAAATTGTGAACTTTTGTTATGGTTATCCTTGTTAGTTGTTAAGGGAAAAAAAAaCAAAAaCAAAAACAGAATATCGCTGACACTGGCACTTAAATAAAAACCTTCTACGACATTTGATTTCGTAAACAATTGTCACTATGTAATTCACATAACACAATGTATGTCTCATTCCTTCATCGATTTAGCTAATCATTTTtATTGTGGTGTAGCAAGAAGTTTTGAGCATCCTTTTTtCTTGCTGAAGAACATTGTGAAATTCATGTGTCGGAGATAGCATGTTAAATACCTGGTTTGATACCGTTGCATAACTTTTCGAGTTGGATCTATATCATATTTACTTTTCCAGCAAAGTAGCTTGGGCTAATAATACTGGATTTATGCATGGTTAAAGCAAACAGTAGCATGCAGAGTTGGATTTTCTTTTTTAAATACTTTCTTTTTTCTTGCCTTTATGCCATGGATATTAGAGATTTTTGTAATTTATTCTTTTTCCCTCTAAGTTATGAGATGAAAGTAGTGCTTTAGTTCTTGAAGCAGAAGTGCATTCCTCCTTCAATATCAGTGGTTTCAAATTGCAAAAAAGTGCATTGTAATTATTTATCCATGTTGTTCTATTGCACCTCGTGGGATTACTGGCTTGATGCATCGTTTTTCATTCCAATTTATAGGTTTTTGTGGGGAGACCGAAGAGGAACATGCTGATACTCTGAGTTTGATTAATGAAGTTGGTTACGATATGGCTTACATGTTTGCATATAGCATGAGGGAAAAGACTCATGCCCATCGGAACTATGTCGATGACATTCCAGAGGAAGTGAAGCAGAGAAGACTTACTGAACTCATTGAAGCTTTTCGAAACAGTACAGGACGGTGTTATGATTCACAAATTGGTAGTGTCCAATTGGTGCTTGTGGAAGGACCAAACAAGAGAGCCCCAGAGACAGAAATGATCGGCAAGAGCGACAGAAGTCATAGAGTTTCGTTTGTTAATGTCGCAGTTCCACATCGAGATGGTGATTATGATGGACATCGGAAAGCAATGGTTGGTGACTTTGTGGAAGTCCGTATATCAAAATCGACCAGAGCATCATTGTTCGGAGAGGCTCTTGCCATTACAAAACTGAGCTCGTTCTACAACGGTATGGATGAACCAATTGCCTGTATGAGCTGAAATTCTAACTAATTTAGACCATTTTTACTTTTTCTAAAATAGTGTTGCTTGAAGTCTTCAACTACCTCTTTAATACCCGAGGGGTGCTTTGTTGTTATTATTATTTTGTTTTTGATTAGATTATCATCGCACCTATTTGAATGTACAAAAATATAATTTTAGCTTTGGTTACTTGTGATTTATGAAGAGAGG

mRNA sequence

ATGACCAGATCCTTACCAGCTTCTGACGTGCCTTTAAGCGGTCGTGTTTATCATGAAACCTATGGATGTCAAATGAATATTAATGATATGGAGGTAGTTCTATCTATCATGAAAAAAGCTGGATACAGTGAAACTGTTGACACTCCTGAGACTGCAGAGATCATTTTTATCAACACTTGTGCTATCAGGGACAATGCTGAACAGAAGGTGTGGCAGAGACTTAATTACTTTTGGTTTCTGAAGAGAAATTGGAAAAGTAATGTTGCTACCGGAAGATCACAGTCTCGACATCCTCCAAAAGTAGTTGTTTTGGGGTGTATGGCTGAGAGGTTGAAAGATAAAATACTAGATTCAGACAAGATGGTTGATGTGGTTTGCGGACCTGATGCTTACAGAGATCTACCAAGATTATTGGATGAAGTAGATTATGGTCAGAAGGGAATAAACACACTTCTTTCCCTTGAAGAAACTTATGCTGATATAAGTCCTGTACGAATAGCCAAAAATTCAGTCACTGCATTTGTATCTGTGATGAGAGGTTGCAACAATATGTGCTCATTCTGCATTGTTCCTTTTACCAGGGGCAGAGAGCGGTCACGTCCTGTTGAATCAATTGTGTGTGAGGTTAGAGAACTTTATAGAGAAGGTGTGAAAGAGGTAACTCTTCTTGGTCAGAACGTTAACAGCTATAACGATACCACTGAGAATGCAGACATAGAACCAGGAACTAATTGGAAGCTCAGTGATGGATTTTCCACCATAGCCAAGGTTAAGAAAATGGGTTTGCGTTTTTCCGATCTCTTAGATCGTCTATCCACTGAATTTCCTGAGATGAGGTTCAGATACACATCTCCACATCCTAAAGACTTTCCCGATGAATTCCTTTATTTAATGCGAGACAGACACAACATTTGCAAAAGCATCCATTTACCTGCTCAGAGTGGGAATAGCATAGTTCTTGAAAGAATGCGTAGGGGATACACCCGAGAGGCCTACTTGGATCTTGTGCACAAGATTCGATCAATCATTCCAGATGTGGGAATAACTAGTGATTTCATTTGTGGTTTTTGTGGGGAGACCGAAGAGGAACATGCTGATACTCTGAGTTTGATTAATGAAGTTGGTTACGATATGGCTTACATGTTTGCATATAGCATGAGGGAAAAGACTCATGCCCATCGGAACTATGTCGATGACATTCCAGAGGAAGTGAAGCAGAGAAGACTTACTGAACTCATTGAAGCTTTTCGAAACAGTACAGGACGGTGTTATGATTCACAAATTGGTAGTGTCCAATTGGTGCTTGTGGAAGGACCAAACAAGAGAGCCCCAGAGACAGAAATGATCGGCAAGAGCGACAGAAGTCATAGAGTTTCGTTTGTTAATGTCGCAGTTCCACATCGAGATGGTGATTATGATGGACATCGGAAAGCAATGGTTGGTGACTTTGTGGAAGTCCGTATATCAAAATCGACCAGAGCATCATTGTTCGGAGAGGCTCTTGCCATTACAAAACTGAGCTCGTTCTACAACGGTATGGATGAACCAATTGCCTGTATGAGCTGAAATTCTAACTAATTTAGACCATTTTTACTTTTTCTAAAATAGTGTTGCTTGAAGTCTTCAACTACCTCTTTAATACCCGAGGGGTGCTTTGTTGTTATTATTATTTTGTTTTTGATTAGATTATCATCGCACCTATTTGAATGTACAAAAATATAATTTTAGCTTTGGTTACTTGTGATTTATGAAGAGAGG

Coding sequence (CDS)

ATGACCAGATCCTTACCAGCTTCTGACGTGCCTTTAAGCGGTCGTGTTTATCATGAAACCTATGGATGTCAAATGAATATTAATGATATGGAGGTAGTTCTATCTATCATGAAAAAAGCTGGATACAGTGAAACTGTTGACACTCCTGAGACTGCAGAGATCATTTTTATCAACACTTGTGCTATCAGGGACAATGCTGAACAGAAGGTGTGGCAGAGACTTAATTACTTTTGGTTTCTGAAGAGAAATTGGAAAAGTAATGTTGCTACCGGAAGATCACAGTCTCGACATCCTCCAAAAGTAGTTGTTTTGGGGTGTATGGCTGAGAGGTTGAAAGATAAAATACTAGATTCAGACAAGATGGTTGATGTGGTTTGCGGACCTGATGCTTACAGAGATCTACCAAGATTATTGGATGAAGTAGATTATGGTCAGAAGGGAATAAACACACTTCTTTCCCTTGAAGAAACTTATGCTGATATAAGTCCTGTACGAATAGCCAAAAATTCAGTCACTGCATTTGTATCTGTGATGAGAGGTTGCAACAATATGTGCTCATTCTGCATTGTTCCTTTTACCAGGGGCAGAGAGCGGTCACGTCCTGTTGAATCAATTGTGTGTGAGGTTAGAGAACTTTATAGAGAAGGTGTGAAAGAGGTAACTCTTCTTGGTCAGAACGTTAACAGCTATAACGATACCACTGAGAATGCAGACATAGAACCAGGAACTAATTGGAAGCTCAGTGATGGATTTTCCACCATAGCCAAGGTTAAGAAAATGGGTTtGCGTTTTTCCGATCTCTTAGATCGTCTATCCACTGAATTTCCTGAGATGAGGTTCAGATACACATCTCCACATCCTAAAGACTTTCCCGATGAATTCCTTTATTTAATGCGAGACAGACACAACATTTGCAAAAGCATCCATTTACCTGCTCAGAGTGGGAATAGCATAGTTCTTGAAAGAATGCGTAGGGGATACACCCGAGAGGCCTACTTGGATCTTGTGCACAAGATTCGATCAATCATTCCAGATGTGGGAATAACTAGTGATTTCATTTGTGGTTTTTGTGGGGAGACCGAAGAGGAACATGCTGATACTCTGAGTTTGATTAATGAAGTTGGTTACGATATGGCTTACATGTTTGCATATAGCATGAGGGAAAAGACTCATGCCCATCGGAACTATGTCGATGACATTCCAGAGGAAGTGAAGCAGAGAAGACTTACTGAACTCATTGAAGCTTTTCGAAACAGTACAGGACGGTGTTATGATTCACAAATTGGTAGTGTCCAATTGGTGCTTGTGGAAGGACCAAACAAGAGAGCCCCAGAGACAGAAATGATCGGCAAGAGCGACAGAAGTCATAGAGTTTCGTTTGTTAATGTCGCAGTTCCACATCGAGATGGTGATTATGATGGACATCGGAAAGCAATGGTTGGTGACTTTGTGGAAGTCCGTATATCAAAATCGACCAGAGCATCATTGTTCGGAGAGGCTCTTGCCATTACAAAACTGAGCTCGTTCTACAACGGTATGGATGAACCAATTGCCTGTATGAGCTGA

Protein sequence

MTRSLPASDVPLSGRVYHETYGCQMNINDMEVVLSIMKKAGYSETVDTPETAEIIFINTCAIRDNAEQKVWQRLNYFWFLKRNWKSNVATGRSQSRHPPKVVVLGCMAERLKDKILDSDKMVDVVCGPDAYRDLPRLLDEVDYGQKGINTLLSLEETYADISPVRIAKNSVTAFVSVMRGCNNMCSFCIVPFTRGRERSRPVESIVCEVRELYREGVKEVTLLGQNVNSYNDTTENADIEPGTNWKLSDGFSTIAKVKKMGLRFSDLLDRLSTEFPEMRFRYTSPHPKDFPDEFLYLMRDRHNICKSIHLPAQSGNSIVLERMRRGYTREAYLDLVHKIRSIIPDVGITSDFICGFCGETEEEHADTLSLINEVGYDMAYMFAYSMREKTHAHRNYVDDIPEEVKQRRLTELIEAFRNSTGRCYDSQIGSVQLVLVEGPNKRAPETEMIGKSDRSHRVSFVNVAVPHRDGDYDGHRKAMVGDFVEVRISKSTRASLFGEALAITKLSSFYNGMDEPIACMS*
BLAST of Cucsa.184640 vs. Swiss-Prot
Match: CK5P1_ARATH (CDK5RAP1-like protein OS=Arabidopsis thaliana GN=At4g36390 PE=2 SV=1)

HSP 1 Score: 823.5 bits (2126), Expect = 1.3e-237
Identity = 403/508 (79.33%), Postives = 449/508 (88.39%), Query Frame = 1

Query: 8   SDVPLSGRVYHETYGCQMNINDMEVVLSIMKKAGYSETVDTPETAEIIFINTCAIRDNAE 67
           SD+   GR+YHETYGCQMNINDME+VL+IMK +GY E V  PE+AE+IF+NTCAIR+NAE
Sbjct: 123 SDIASKGRIYHETYGCQMNINDMEIVLAIMKNSGYKEVVTDPESAEVIFVNTCAIRENAE 182

Query: 68  QKVWQRLNYFWFLKRNWKSNVATGRSQSRHPPKVVVLGCMAERLKDKILDSDKMVDVVCG 127
           Q+VWQRLNYFWFLKR WK N ATGR++S  PPKVVVLGCMAERLKDKILDSDKMVDVVCG
Sbjct: 183 QRVWQRLNYFWFLKREWKVNAATGRAKSLKPPKVVVLGCMAERLKDKILDSDKMVDVVCG 242

Query: 128 PDAYRDLPRLLDEVDYGQKGINTLLSLEETYADISPVRIAKNSVTAFVSVMRGCNNMCSF 187
           PDAYRDLPRLL+EVDYGQKGINTLLSLEETYADISPVRI++NS+TAFVSVMRGCNNMC+F
Sbjct: 243 PDAYRDLPRLLEEVDYGQKGINTLLSLEETYADISPVRISENSITAFVSVMRGCNNMCAF 302

Query: 188 CIVPFTRGRERSRPVESIVCEVRELYREGVKEVTLLGQNVNSYNDTTENADIEPGTNWKL 247
           CIVPFTRGRERSRPVESI+ EV EL+  GVKEVTLLGQNVNSYND  ++AD E G NW+ 
Sbjct: 303 CIVPFTRGRERSRPVESIIREVGELWESGVKEVTLLGQNVNSYND--DSADRESGANWEY 362

Query: 248 SDGFSTIAKVKKMGLRFSDLLDRLSTEFPEMRFRYTSPHPKDFPDEFLYLMRDRHNICKS 307
           S+GFS+  KVK MGLRF+DLLDRLS EFPEMRFR+TSPHPKD+PDE LYLMRDRHNIC  
Sbjct: 363 SEGFSSRCKVKNMGLRFADLLDRLSVEFPEMRFRFTSPHPKDYPDELLYLMRDRHNICNL 422

Query: 308 IHLPAQSGNSIVLERMRRGYTREAYLDLVHKIRSIIPDVGITSDFICGFCGETEEEHADT 367
           IHLPAQSGNS +LE+MRRGYTREAYLDLV KIRSIIPDV ITSDFI GFCGETEEEH +T
Sbjct: 423 IHLPAQSGNSRILEQMRRGYTREAYLDLVKKIRSIIPDVAITSDFITGFCGETEEEHQET 482

Query: 368 LSLINEVGYDMAYMFAYSMREKTHAHRNYVDDIPEEVKQRRLTELIEAFRNSTGRCYDSQ 427
           LSL+  VGYDMAYMFAYSMREKTHAHRNY DD+PEEVKQRRLTELI+AFR +TG CYDSQ
Sbjct: 483 LSLVRAVGYDMAYMFAYSMREKTHAHRNYTDDVPEEVKQRRLTELIDAFRETTGPCYDSQ 542

Query: 428 IGSVQLVLVEGPNKRAPETEMIGKSDRSHRVSFVNVAVPHR----DGDYDGHRKAMVGDF 487
           +GS QLVLVEGPNKRAPETE+IGK+D+ HRVSFV   +  +    DGD D  R   +GDF
Sbjct: 543 VGSTQLVLVEGPNKRAPETELIGKTDKGHRVSFVTKPLFDKACLLDGD-DLKRNPGIGDF 602

Query: 488 VEVRISKSTRASLFGEALAITKLSSFYN 512
           VEV+I KSTRASLFGEALAI+K+S F++
Sbjct: 603 VEVQIEKSTRASLFGEALAISKMSLFHD 627

BLAST of Cucsa.184640 vs. Swiss-Prot
Match: CK5P1_ORYSJ (CDK5RAP1-like protein OS=Oryza sativa subsp. japonica GN=Os11g0592800 PE=2 SV=1)

HSP 1 Score: 819.3 bits (2115), Expect = 2.4e-236
Identity = 393/497 (79.07%), Postives = 444/497 (89.34%), Query Frame = 1

Query: 14  GRVYHETYGCQMNINDMEVVLSIMKKAGYSETVDTPETAEIIFINTCAIRDNAEQKVWQR 73
           GR+YHETYGCQMN+NDME+VLSIMK  GY E V  PE+AEIIFINTCAIRDNAEQKVWQR
Sbjct: 93  GRIYHETYGCQMNVNDMEIVLSIMKNEGYDEIVPDPESAEIIFINTCAIRDNAEQKVWQR 152

Query: 74  LNYFWFLKRNWKSNVATGRSQSRHPPKVVVLGCMAERLKDKILDSDKMVDVVCGPDAYRD 133
           LNYFWFLKR WK+NVA GRS+S  PPK+ VLGCMAERLK+KILDSDKMVDVVCGPDAYRD
Sbjct: 153 LNYFWFLKRQWKANVAAGRSRSLRPPKIAVLGCMAERLKEKILDSDKMVDVVCGPDAYRD 212

Query: 134 LPRLLDEVDYGQKGINTLLSLEETYADISPVRIAKNSVTAFVSVMRGCNNMCSFCIVPFT 193
           LPRLL EVDYGQKGINTLLSLEETYADI+PVRI+ NSVTAFVS+MRGCNNMCSFCIVPFT
Sbjct: 213 LPRLLQEVDYGQKGINTLLSLEETYADITPVRISDNSVTAFVSIMRGCNNMCSFCIVPFT 272

Query: 194 RGRERSRPVESIVCEVRELYREGVKEVTLLGQNVNSYNDTTENADIEPGTNWKLSDGFST 253
           RGRERSRPV SIV EV EL++ GVKEV LLGQNVNSYNDT+E  ++EPG NW+LS+GFS+
Sbjct: 273 RGRERSRPVSSIVREVGELWKAGVKEVMLLGQNVNSYNDTSEVEELEPGKNWELSEGFSS 332

Query: 254 IAKVKKMGLRFSDLLDRLSTEFPEMRFRYTSPHPKDFPDEFLYLMRDRHNICKSIHLPAQ 313
           + KVK MGLRF+DLLD+LS E+PEMRFR+TSPHPKD+PDE LYLMRDRHN+CK IH+PAQ
Sbjct: 333 MCKVKNMGLRFADLLDQLSLEYPEMRFRFTSPHPKDYPDELLYLMRDRHNVCKLIHMPAQ 392

Query: 314 SGNSIVLERMRRGYTREAYLDLVHKIRSIIPDVGITSDFICGFCGETEEEHADTLSLINE 373
           SG+S VLERMRRGYTREAYL+LV KIRSIIPDVG++SDFI GFCGETEEEHA+TL+L+  
Sbjct: 393 SGSSAVLERMRRGYTREAYLELVQKIRSIIPDVGLSSDFISGFCGETEEEHAETLTLVRA 452

Query: 374 VGYDMAYMFAYSMREKTHAHRNYVDDIPEEVKQRRLTELIEAFRNSTGRCYDSQIGSVQL 433
           VGYDMAYMFAYSMREKTHAHRNYVDD+P++VKQRRL ELI  FR +T + YDSQ+G+VQL
Sbjct: 453 VGYDMAYMFAYSMREKTHAHRNYVDDVPDDVKQRRLAELISTFRETTAKIYDSQVGTVQL 512

Query: 434 VLVEGPNKRAPETEMIGKSDRSHRVSFVNVAVPHRDGDYDGHRKAMVGDFVEVRISKSTR 493
           VLVEGPNKRAPETEMIGK+DR HRVSF +V VPH   + D  RK +VGDF+EV+I+KS+ 
Sbjct: 513 VLVEGPNKRAPETEMIGKTDRGHRVSFASVPVPH-TFEGDELRKPVVGDFIEVKITKSST 572

Query: 494 ASLFGEALAITKLSSFY 511
           ASL G+ +A T LS FY
Sbjct: 573 ASLSGDVIARTSLSKFY 588

BLAST of Cucsa.184640 vs. Swiss-Prot
Match: CK5P1_RAT (CDK5 regulatory subunit-associated protein 1 OS=Rattus norvegicus GN=Cdk5rap1 PE=1 SV=1)

HSP 1 Score: 511.9 bits (1317), Expect = 8.1e-144
Identity = 271/493 (54.97%), Postives = 354/493 (71.81%), Query Frame = 1

Query: 15  RVYHETYGCQMNINDMEVVLSIMKKAGYSETVDTPETAEIIFINTCAIRDNAEQKVWQRL 74
           +VY ETYGCQMN+ND E+  SI++K+GY  T +  E A++I + TC+IR+ AEQ +W RL
Sbjct: 98  KVYLETYGCQMNVNDTEIAWSILQKSGYLRTSNLQE-ADVILLVTCSIREKAEQTIWNRL 157

Query: 75  NYFWFLKRNWKSNVATGRSQSRHPPKVVVLGCMAERLKDKILDSDKMVDVVCGPDAYRDL 134
           +    LK          R +SR P ++ +LGCMAERLK +IL+ +KMVD++ GPDAYRDL
Sbjct: 158 HQLKVLKAK--------RPRSRVPLRIGILGCMAERLKGEILNREKMVDLLAGPDAYRDL 217

Query: 135 PRLLDEVDYGQKGINTLLSLEETYADISPVRIAKNSVTAFVSVMRGCNNMCSFCIVPFTR 194
           PRLL  V+ GQ+  N LLSL+ETYADI PV+ + ++ +AFVS+MRGC+NMCS+CIVPFTR
Sbjct: 218 PRLLAVVESGQQAANVLLSLDETYADIMPVQTSPSATSAFVSIMRGCDNMCSYCIVPFTR 277

Query: 195 GRERSRPVESIVCEVRELYREGVKEVTLLGQNVNSYNDTTENADIEPGTNWKLSDGFSTI 254
           GRERSRPV SI+ EVR+L  +G+KEVTLLGQNVNS+ D +E      G+   LS GF+T 
Sbjct: 278 GRERSRPVASILDEVRKLSEQGLKEVTLLGQNVNSFRDNSEVQFSSTGSA-NLSRGFTTN 337

Query: 255 AKVKKMGLRFSDLLDRLSTEFPEMRFRYTSPHPKDFPDEFLYLMRDRHNICKSIHLPAQS 314
            K K+ GLRFS LLD++S   PEMR R+TSPHPKDFPDE L L+R+RHNICK IHLPAQS
Sbjct: 338 YKPKQGGLRFSHLLDQVSRIDPEMRIRFTSPHPKDFPDEVLQLIRERHNICKQIHLPAQS 397

Query: 315 GNSIVLERMRRGYTREAYLDLVHKIRSIIPDVGITSDFICGFCGETEEEHADTLSLINEV 374
           G+S VLE MRRGY+REAY+ LVH IR  IP VG++SDFI GFCGETE++H  T+SL+ EV
Sbjct: 398 GSSRVLEAMRRGYSREAYVALVHHIREAIPGVGLSSDFITGFCGETEDDHLQTVSLLREV 457

Query: 375 GYDMAYMFAYSMREKTHAHRNYVDDIPEEVKQRRLTELIEAFRNSTGRCYDSQIGSVQLV 434
            Y+  ++FAYSMR+KT A+    DD+PEEVK RRL ELI  FR    +   + +G  QLV
Sbjct: 458 QYNTGFLFAYSMRQKTRAYHRLKDDVPEEVKLRRLEELITVFREEASKVNATSVGCTQLV 517

Query: 435 LVEGPNKRAPETEMIGKSDRSHRVSFVNVAVPHRDGDYDGHR-KAMVGDFVEVRISKSTR 494
           LVEG +KR+  T++ G++D + +V F +  V   D    G + +A  GD+V V+I  ++ 
Sbjct: 518 LVEGFSKRS-TTDLCGRNDANLKVIFPDAEV--EDITDPGLKVRAQPGDYVLVKIISASS 577

Query: 495 ASLFGEALAITKL 507
            +L G  L  T +
Sbjct: 578 QTLKGHILCRTTM 577

BLAST of Cucsa.184640 vs. Swiss-Prot
Match: CK5P1_MOUSE (CDK5 regulatory subunit-associated protein 1 OS=Mus musculus GN=Cdk5rap1 PE=2 SV=2)

HSP 1 Score: 510.8 bits (1314), Expect = 1.8e-143
Identity = 269/493 (54.56%), Postives = 355/493 (72.01%), Query Frame = 1

Query: 15  RVYHETYGCQMNINDMEVVLSIMKKAGYSETVDTPETAEIIFINTCAIRDNAEQKVWQRL 74
           +VY ETYGCQMN+ND E+  SI++K+GY  T +  E A++I + TC+IR+ AEQ +W RL
Sbjct: 100 KVYLETYGCQMNVNDTEIAWSILQKSGYLRTSNLQE-ADVILLVTCSIREKAEQTIWNRL 159

Query: 75  NYFWFLKRNWKSNVATGRSQSRHPPKVVVLGCMAERLKDKILDSDKMVDVVCGPDAYRDL 134
           +    LK        T R +SR P ++ +LGCMAERLK +IL+ +KMVD++ GPDAYRDL
Sbjct: 160 HQLKVLK--------TKRPRSRVPLRIGILGCMAERLKGEILNREKMVDLLAGPDAYRDL 219

Query: 135 PRLLDEVDYGQKGINTLLSLEETYADISPVRIAKNSVTAFVSVMRGCNNMCSFCIVPFTR 194
           PRLL  V+ GQ+  N LLSL+ETYADI PV+ + ++ +AFVS+MRGC+NMCS+CIVPFTR
Sbjct: 220 PRLLAVVESGQQAANVLLSLDETYADIMPVQTSPSATSAFVSIMRGCDNMCSYCIVPFTR 279

Query: 195 GRERSRPVESIVCEVRELYREGVKEVTLLGQNVNSYNDTTENADIEPGTNWKLSDGFSTI 254
           GRERSRPV SI+ EVR+L  +G+KEVTLLGQNVNS+ D +E      G+   LS GF+T 
Sbjct: 280 GRERSRPVASILDEVRKLSEQGLKEVTLLGQNVNSFRDNSEVQFNNAGSA-NLSRGFTTN 339

Query: 255 AKVKKMGLRFSDLLDRLSTEFPEMRFRYTSPHPKDFPDEFLYLMRDRHNICKSIHLPAQS 314
            K K+ GLRFS LLD++S   PEMR R+TSPHPKDFPDE L L+R+RHNICK IHLPAQS
Sbjct: 340 YKPKQGGLRFSHLLDQVSRIDPEMRIRFTSPHPKDFPDEVLQLIRERHNICKQIHLPAQS 399

Query: 315 GNSIVLERMRRGYTREAYLDLVHKIRSIIPDVGITSDFICGFCGETEEEHADTLSLINEV 374
           G+S VL+ MRRGY+REAY+ LVH +R  IP V ++SDFI GFCGETE++H  T+SL+ EV
Sbjct: 400 GSSRVLDAMRRGYSREAYVALVHHVRETIPGVSLSSDFITGFCGETEDDHRQTVSLLREV 459

Query: 375 GYDMAYMFAYSMREKTHAHRNYVDDIPEEVKQRRLTELIEAFRNSTGRCYDSQIGSVQLV 434
            Y+  ++FAYSMR+KT A+    DD+PEEVK RRL ELI  FR    +   + +G  QLV
Sbjct: 460 QYNTGFLFAYSMRQKTRAYHRLKDDVPEEVKLRRLEELITVFREEASKANKTSVGCSQLV 519

Query: 435 LVEGPNKRAPETEMIGKSDRSHRVSFVNVAVPHRDGDYDGHR-KAMVGDFVEVRISKSTR 494
           LVEG +KR+  T++ G++D + +V F +  V   D    G + +A  GD+V V+I+ ++ 
Sbjct: 520 LVEGFSKRS-TTDLCGRNDANLKVIFPDAEV--EDITNPGLKVRAQPGDYVLVKITSASS 579

Query: 495 ASLFGEALAITKL 507
            +L G  L  T +
Sbjct: 580 QTLKGHILCRTTM 579

BLAST of Cucsa.184640 vs. Swiss-Prot
Match: CK5P1_DROME (CDK5RAP1-like protein OS=Drosophila melanogaster GN=CG6345 PE=2 SV=1)

HSP 1 Score: 504.6 bits (1298), Expect = 1.3e-141
Identity = 259/498 (52.01%), Postives = 343/498 (68.88%), Query Frame = 1

Query: 15  RVYHETYGCQMNINDMEVVLSIMKKAGYSETVDTPETAEIIFINTCAIRDNAEQKVWQRL 74
           +V+ E YGCQMN ND EVV SI+K+ GY    + PE A++I + TCA+RD AEQ++  RL
Sbjct: 93  KVHFEVYGCQMNTNDTEVVFSILKENGYLRCQE-PEEADVIMLVTCAVRDGAEQRIRNRL 152

Query: 75  NYFWFLKRNWKSNVATGRSQSRHPPKVVVLGCMAERLKDKILDSDKMVDVVCGPDAYRDL 134
            +   +K          RS  RHP ++ +LGCMAERLK+K+L+ ++ VDV+ GPD+Y+DL
Sbjct: 153 KHLRAMKNK--------RSTRRHPLQLTLLGCMAERLKEKLLEQEQCVDVIAGPDSYKDL 212

Query: 135 PRLLD-EVDYGQKGINTLLSLEETYADISPVRIAKNSVTAFVSVMRGCNNMCSFCIVPFT 194
           PRLL     YG   IN LLSL+ETYAD+ PVR+   S TAFVS+MRGC+NMC++CIVPFT
Sbjct: 213 PRLLAISRHYGNSAINVLLSLDETYADVMPVRLNSESPTAFVSIMRGCDNMCTYCIVPFT 272

Query: 195 RGRERSRPVESIVCEVRELYREGVKEVTLLGQNVNSYNDTTENADIEPGTNWKLSDGFST 254
           RGRERSRP+ SIV EV+ L  +GVKEVTLLGQNVNSY D T   + +         GFST
Sbjct: 273 RGRERSRPLASIVAEVKALAEQGVKEVTLLGQNVNSYRDRTAQEE-QDSLKATPVPGFST 332

Query: 255 IAKVKKMGLRFSDLLDRLSTEFPEMRFRYTSPHPKDFPDEFLYLMRDRHNICKSIHLPAQ 314
           + K K  G  F+ LL  ++   P+MR R+TSPHPKDF DE L ++RD  N+CK +HLPAQ
Sbjct: 333 VYKPKTGGTPFAALLRSVAQAVPDMRIRFTSPHPKDFSDEVLEVIRDHPNVCKQLHLPAQ 392

Query: 315 SGNSIVLERMRRGYTREAYLDLVHKIRSIIPDVGITSDFICGFCGETEEEHADTLSLINE 374
           SGN+ VLERMRRGY+REAYL+LV  IR  +P+VG++SDFICGFCGETEEE  DT+SLI +
Sbjct: 393 SGNTQVLERMRRGYSREAYLELVQHIRQFLPNVGLSSDFICGFCGETEEEFQDTVSLIQQ 452

Query: 375 VGYDMAYMFAYSMREKTHAHRNYVDDIPEEVKQRRLTELIEAFRNSTGRCYDSQIGSVQL 434
           V Y++AY+FAYSMREKT AHR Y DD+P  VK  RL  +++ FR    + +    G  QL
Sbjct: 453 VQYNVAYLFAYSMREKTTAHRRYKDDVPINVKNERLQRMVQVFREGATQLHRKMEGQEQL 512

Query: 435 VLVEGPNKRAPETEMIGKSDRSHRVSFVNVAVPHRDGDYDGHRKAMVGDFVEVRISKSTR 494
           +L+EG +KR+ +    G++D + +V   ++ VP   GD    +   VGDF+ VRI +S  
Sbjct: 513 ILIEGKSKRS-DAHWFGRNDANIKVIVPSIYVP-ISGDSTARKSFGVGDFLAVRIEESNS 572

Query: 495 ASLFGEALAITKLSSFYN 512
             L G  L ++ ++ F++
Sbjct: 573 QVLKGTPLELSNITDFHS 578

BLAST of Cucsa.184640 vs. TrEMBL
Match: A0A0A0KDY2_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_6G411190 PE=4 SV=1)

HSP 1 Score: 1053.5 bits (2723), Expect = 8.4e-305
Identity = 519/520 (99.81%), Postives = 519/520 (99.81%), Query Frame = 1

Query: 2   TRSLPASDVPLSGRVYHETYGCQMNINDMEVVLSIMKKAGYSETVDTPETAEIIFINTCA 61
           T SLPASDVPLSGRVYHETYGCQMNINDMEVVLSIMKKAGYSETVDTPETAEIIFINTCA
Sbjct: 116 TESLPASDVPLSGRVYHETYGCQMNINDMEVVLSIMKKAGYSETVDTPETAEIIFINTCA 175

Query: 62  IRDNAEQKVWQRLNYFWFLKRNWKSNVATGRSQSRHPPKVVVLGCMAERLKDKILDSDKM 121
           IRDNAEQKVWQRLNYFWFLKRNWKSNVATGRSQSRHPPKVVVLGCMAERLKDKILDSDKM
Sbjct: 176 IRDNAEQKVWQRLNYFWFLKRNWKSNVATGRSQSRHPPKVVVLGCMAERLKDKILDSDKM 235

Query: 122 VDVVCGPDAYRDLPRLLDEVDYGQKGINTLLSLEETYADISPVRIAKNSVTAFVSVMRGC 181
           VDVVCGPDAYRDLPRLLDEVDYGQKGINTLLSLEETYADISPVRIAKNSVTAFVSVMRGC
Sbjct: 236 VDVVCGPDAYRDLPRLLDEVDYGQKGINTLLSLEETYADISPVRIAKNSVTAFVSVMRGC 295

Query: 182 NNMCSFCIVPFTRGRERSRPVESIVCEVRELYREGVKEVTLLGQNVNSYNDTTENADIEP 241
           NNMCSFCIVPFTRGRERSRPVESIVCEVRELYREGVKEVTLLGQNVNSYNDTTENADIEP
Sbjct: 296 NNMCSFCIVPFTRGRERSRPVESIVCEVRELYREGVKEVTLLGQNVNSYNDTTENADIEP 355

Query: 242 GTNWKLSDGFSTIAKVKKMGLRFSDLLDRLSTEFPEMRFRYTSPHPKDFPDEFLYLMRDR 301
           GTNWKLSDGFSTIAKVKKMGLRFSDLLDRLSTEFPEMRFRYTSPHPKDFPDEFLYLMRDR
Sbjct: 356 GTNWKLSDGFSTIAKVKKMGLRFSDLLDRLSTEFPEMRFRYTSPHPKDFPDEFLYLMRDR 415

Query: 302 HNICKSIHLPAQSGNSIVLERMRRGYTREAYLDLVHKIRSIIPDVGITSDFICGFCGETE 361
           HNICKSIHLPAQSGNSIVLERMRRGYTREAYLDLVHKIRSIIPDVGITSDFICGFCGETE
Sbjct: 416 HNICKSIHLPAQSGNSIVLERMRRGYTREAYLDLVHKIRSIIPDVGITSDFICGFCGETE 475

Query: 362 EEHADTLSLINEVGYDMAYMFAYSMREKTHAHRNYVDDIPEEVKQRRLTELIEAFRNSTG 421
           EEHADTLSLINEVGYDMAYMFAYSMREKTHAHRNYVDDIPEEVKQRRLTELIEAFRNSTG
Sbjct: 476 EEHADTLSLINEVGYDMAYMFAYSMREKTHAHRNYVDDIPEEVKQRRLTELIEAFRNSTG 535

Query: 422 RCYDSQIGSVQLVLVEGPNKRAPETEMIGKSDRSHRVSFVNVAVPHRDGDYDGHRKAMVG 481
           RCYDSQIGSVQLVLVEGPNKRAPETEMIGKSDRSHRVSFVNVAVPHRDGDYDGHRKAMVG
Sbjct: 536 RCYDSQIGSVQLVLVEGPNKRAPETEMIGKSDRSHRVSFVNVAVPHRDGDYDGHRKAMVG 595

Query: 482 DFVEVRISKSTRASLFGEALAITKLSSFYNGMDEPIACMS 522
           DFVEVRISKSTRASLFGEALAITKLSSFYNGMDEPIACMS
Sbjct: 596 DFVEVRISKSTRASLFGEALAITKLSSFYNGMDEPIACMS 635

BLAST of Cucsa.184640 vs. TrEMBL
Match: B9HFB0_POPTR (Uncharacterized protein OS=Populus trichocarpa GN=POPTR_0007s13510g PE=4 SV=1)

HSP 1 Score: 880.6 bits (2274), Expect = 9.7e-253
Identity = 431/525 (82.10%), Postives = 474/525 (90.29%), Query Frame = 1

Query: 5   LPASDVPLS------GRVYHETYGCQMNINDMEVVLSIMKKAGYSETVDTPETAEIIFIN 64
           +PA +VP        GR+YHETYGCQMN+NDME+VLSIMK AGYSE VD PE+AEIIFIN
Sbjct: 99  VPAIEVPPDIEILPRGRIYHETYGCQMNVNDMEIVLSIMKNAGYSEIVDVPESAEIIFIN 158

Query: 65  TCAIRDNAEQKVWQRLNYFWFLKRNWKSNVATGRSQSRHPPKVVVLGCMAERLKDKILDS 124
           TCAIRDNAEQKVWQRLNYFWFLKR+WKSNVA GRSQS HPPKVVVLGCMAERLKDKILD+
Sbjct: 159 TCAIRDNAEQKVWQRLNYFWFLKRHWKSNVAIGRSQSHHPPKVVVLGCMAERLKDKILDA 218

Query: 125 DKMVDVVCGPDAYRDLPRLLDEVDYGQKGINTLLSLEETYADISPVRIAKNSVTAFVSVM 184
           DKMVDVVCGPDAYRDLPRLL+EV+YGQKGINTLLSLEETYADISPVRI+KNS+ AFVSVM
Sbjct: 219 DKMVDVVCGPDAYRDLPRLLEEVEYGQKGINTLLSLEETYADISPVRISKNSINAFVSVM 278

Query: 185 RGCNNMCSFCIVPFTRGRERSRPVESIVCEVRELYREGVKEVTLLGQNVNSYNDTTE-NA 244
           RGCNNMCSFCIVPFTRGRERSRPVESIV EV EL++EGVKEVTLLGQNVNSYND +E   
Sbjct: 279 RGCNNMCSFCIVPFTRGRERSRPVESIVKEVAELWKEGVKEVTLLGQNVNSYNDASEIEK 338

Query: 245 DIEPGTNWKLSDGFSTIAKVKKMGLRFSDLLDRLSTEFPEMRFRYTSPHPKDFPDEFLYL 304
           ++EPGTNWK SDGFS+  KVK++GLRFSDLLDRLSTEFPEMRFRYTSPHPKDFPD+ LY+
Sbjct: 339 EVEPGTNWKYSDGFSSTCKVKRVGLRFSDLLDRLSTEFPEMRFRYTSPHPKDFPDDLLYV 398

Query: 305 MRDRHNICKSIHLPAQSGNSIVLERMRRGYTREAYLDLVHKIRSIIPDVGITSDFICGFC 364
           MR+RHNICKSIHLPAQ+G+S VLERMRRGYTREAYLDLV KIR IIPDVGITSDFICGFC
Sbjct: 399 MRNRHNICKSIHLPAQTGSSTVLERMRRGYTREAYLDLVQKIRRIIPDVGITSDFICGFC 458

Query: 365 GETEEEHADTLSLINEVGYDMAYMFAYSMREKTHAHRNYVDDIPEEVKQRRLTELIEAFR 424
           GETEEEH DTLSL+  VGYDMAYMFAYSMREKTHAHRNYVDD+P+EVK+RRL ELIEAFR
Sbjct: 459 GETEEEHQDTLSLVKAVGYDMAYMFAYSMREKTHAHRNYVDDVPDEVKKRRLAELIEAFR 518

Query: 425 NSTGRCYDSQIGSVQLVLVEGPNKRAPETEMIGKSDRSHRVSFVNVAVPHRDGDYDGHRK 484
            STG+C+DSQIGSVQLVLVEGPNKRAP+TE+IGKSDR HRV F N+ +P+R+ D    R 
Sbjct: 519 ESTGQCFDSQIGSVQLVLVEGPNKRAPDTELIGKSDRGHRVLFTNLPLPNRNEDGSQARN 578

Query: 485 AMVGDFVEVRISKSTRASLFGEALAITKLSSFYNGMDE-PIACMS 522
            MVGD+VEV I KSTRASLFG+ALAIT LSSFY+ +D+  +AC S
Sbjct: 579 PMVGDYVEVLILKSTRASLFGDALAITTLSSFYSNLDQKAVACAS 623

BLAST of Cucsa.184640 vs. TrEMBL
Match: M5WF79_PRUPE (Uncharacterized protein OS=Prunus persica GN=PRUPE_ppa002826mg PE=4 SV=1)

HSP 1 Score: 880.2 bits (2273), Expect = 1.3e-252
Identity = 425/516 (82.36%), Postives = 472/516 (91.47%), Query Frame = 1

Query: 8   SDVPLSGRVYHETYGCQMNINDMEVVLSIMKKAGYSETVDTPETAEIIFINTCAIRDNAE 67
           S+VP  GR+YHETYGCQMNINDME+VLSIMK AGYSE VD PE AE+IFINTCAIRDNAE
Sbjct: 112 SEVPPMGRIYHETYGCQMNINDMEIVLSIMKNAGYSEVVDVPENAEVIFINTCAIRDNAE 171

Query: 68  QKVWQRLNYFWFLKRNWKSNVATGRSQSRHPPKVVVLGCMAERLKDKILDSDKMVDVVCG 127
           QKVWQRLNYFWFLKR+WKSNV  GRSQS+ PPKVVVLGCMAERLKDKILD+DKMVDVVCG
Sbjct: 172 QKVWQRLNYFWFLKRHWKSNVKIGRSQSKRPPKVVVLGCMAERLKDKILDADKMVDVVCG 231

Query: 128 PDAYRDLPRLLDEVDYGQKGINTLLSLEETYADISPVRIAKNSVTAFVSVMRGCNNMCSF 187
           PDAYRDLPRLL++VDYGQKGINTLLSLEETYADISPVRIAKNS++AFVSVMRGCNNMCSF
Sbjct: 232 PDAYRDLPRLLEDVDYGQKGINTLLSLEETYADISPVRIAKNSISAFVSVMRGCNNMCSF 291

Query: 188 CIVPFTRGRERSRPVESIVCEVRELYREGVKEVTLLGQNVNSYNDTTE-NADIEPGTNWK 247
           CIVPFTRGRERSRPVESIV EV EL+ EGVKEVTLLGQNVNSYND +E   ++E GTNW+
Sbjct: 292 CIVPFTRGRERSRPVESIVREVAELWEEGVKEVTLLGQNVNSYNDASEYEKEVETGTNWR 351

Query: 248 LSDGFSTIAKVKKMGLRFSDLLDRLSTEFPEMRFRYTSPHPKDFPDEFLYLMRDRHNICK 307
            S+GFS++ KVKK+G RF+DLLDRLSTEFPEMRFRYTSPHPKDFPDE LY+MRDR N+CK
Sbjct: 352 YSEGFSSMCKVKKVGSRFADLLDRLSTEFPEMRFRYTSPHPKDFPDELLYIMRDRPNVCK 411

Query: 308 SIHLPAQSGNSIVLERMRRGYTREAYLDLVHKIRSIIPDVGITSDFICGFCGETEEEHAD 367
           SIHLPAQ+G+S VLERMRRGY+REAYLDLV KIR IIPDVGI+SDFICGFCGETEEEHAD
Sbjct: 412 SIHLPAQTGSSTVLERMRRGYSREAYLDLVQKIRRIIPDVGISSDFICGFCGETEEEHAD 471

Query: 368 TLSLINEVGYDMAYMFAYSMREKTHAHRNYVDDIPEEVKQRRLTELIEAFRNSTGRCYDS 427
           TLSL+  VGYDMAYMFAYSMREKTHAHRNYVDD+PE+VKQRRLTELI+AFR STG+CYDS
Sbjct: 472 TLSLVKAVGYDMAYMFAYSMREKTHAHRNYVDDVPEKVKQRRLTELIKAFRESTGQCYDS 531

Query: 428 QIGSVQLVLVEGPNKRAPETEMIGKSDRSHRVSFVNVAVPHRDGDYDGHRKAMVGDFVEV 487
           Q+G+ QL+LVEGPNKRAP+TE+IGKSDR HRVSF NV +PHRD D +  R  +VGD+VEV
Sbjct: 532 QVGTTQLILVEGPNKRAPDTELIGKSDRGHRVSFANVLLPHRDADSNEERNPVVGDYVEV 591

Query: 488 RISKSTRASLFGEALAITKLSSFYNGM-DEPIACMS 522
           RI KSTRASLFGEA+AI+KLS FY+ + +E +AC S
Sbjct: 592 RILKSTRASLFGEAVAISKLSLFYDNVEEEAVACGS 627

BLAST of Cucsa.184640 vs. TrEMBL
Match: A5AL29_VITVI (Putative uncharacterized protein OS=Vitis vinifera GN=VITISV_007911 PE=4 SV=1)

HSP 1 Score: 879.8 bits (2272), Expect = 1.7e-252
Identity = 425/513 (82.85%), Postives = 468/513 (91.23%), Query Frame = 1

Query: 7   ASDVPLSGRVYHETYGCQMNINDMEVVLSIMKKAGYSETVDTPETAEIIFINTCAIRDNA 66
           AS++   GR+YHETYGCQMNINDME+VLSIMKKAGY+E V+ PE+AE+IFINTCAIRDNA
Sbjct: 68  ASEILPRGRIYHETYGCQMNINDMEIVLSIMKKAGYNEVVEVPESAEVIFINTCAIRDNA 127

Query: 67  EQKVWQRLNYFWFLKRNWKSNVATGRSQSRHPPKVVVLGCMAERLKDKILDSDKMVDVVC 126
           EQKVWQRLNYFWFLKR+WKSNV+ GR+ S HPPKVVVLGCMAERLKDKILD+DKMVDVVC
Sbjct: 128 EQKVWQRLNYFWFLKRHWKSNVSIGRADSLHPPKVVVLGCMAERLKDKILDADKMVDVVC 187

Query: 127 GPDAYRDLPRLLDEVDYGQKGINTLLSLEETYADISPVRIAKNSVTAFVSVMRGCNNMCS 186
           GPDAYRDLPRLL+EVDYGQKGINTLLSLEETYADISPVRI+KNSVTAFVS+MRGCNNMCS
Sbjct: 188 GPDAYRDLPRLLEEVDYGQKGINTLLSLEETYADISPVRISKNSVTAFVSIMRGCNNMCS 247

Query: 187 FCIVPFTRGRERSRPVESIVCEVRELYREGVKEVTLLGQNVNSYNDTTE-NADIEPGTNW 246
           FCIVPFTRGRERSRPVESIV EV EL++EGVKEV LLGQNVNSYND +  + ++EPG NW
Sbjct: 248 FCIVPFTRGRERSRPVESIVREVAELWKEGVKEVMLLGQNVNSYNDASGFDKEVEPGANW 307

Query: 247 KLSDGFSTIAKVKKMGLRFSDLLDRLSTEFPEMRFRYTSPHPKDFPDEFLYLMRDRHNIC 306
           KLS+GFS+  KVKKMGLRFSDLLDRLSTEFPEMRFRYTSPHPKDFPDE LYLMRDR+N+C
Sbjct: 308 KLSEGFSSKCKVKKMGLRFSDLLDRLSTEFPEMRFRYTSPHPKDFPDELLYLMRDRYNVC 367

Query: 307 KSIHLPAQSGNSIVLERMRRGYTREAYLDLVHKIRSIIPDVGITSDFICGFCGETEEEHA 366
           KSIHLPAQ+G+S VLERMRRGYTREAYLDLV KIR I+PDVGITSDFICGFCGETEEE+A
Sbjct: 368 KSIHLPAQTGSSTVLERMRRGYTREAYLDLVQKIRRIVPDVGITSDFICGFCGETEEEYA 427

Query: 367 DTLSLINEVGYDMAYMFAYSMREKTHAHRNYVDDIPEEVKQRRLTELIEAFRNSTGRCYD 426
           DT+SL+  VGYDMAYMFAYSMREKTHAHRNYVDD+P+ +KQRRL E+IE FR STG+CYD
Sbjct: 428 DTISLVKAVGYDMAYMFAYSMREKTHAHRNYVDDVPDNIKQRRLAEMIEVFRESTGQCYD 487

Query: 427 SQIGSVQLVLVEGPNKRAPETEMIGKSDRSHRVSFVNVAVPHRDGDYDGHRKAMVGDFVE 486
           SQIG+VQLVLVEGPNKRAP+TE+IGK+DR HRVSF N  VPH D D  G++   VGDFVE
Sbjct: 488 SQIGTVQLVLVEGPNKRAPDTELIGKTDRGHRVSFKNTPVPHWDDDVGGNQNPRVGDFVE 547

Query: 487 VRISKSTRASLFGEALAITKLSSFYNGMDEPIA 519
           VRI  STRASLFGEALAITKLS FY+  DE IA
Sbjct: 548 VRILTSTRASLFGEALAITKLSLFYSNADEAIA 580

BLAST of Cucsa.184640 vs. TrEMBL
Match: B9S686_RICCO (Radical sam protein, putative OS=Ricinus communis GN=RCOM_0533210 PE=4 SV=1)

HSP 1 Score: 877.1 bits (2265), Expect = 1.1e-251
Identity = 426/515 (82.72%), Postives = 473/515 (91.84%), Query Frame = 1

Query: 8   SDVPLSGRVYHETYGCQMNINDMEVVLSIMKKAGYSETVDTPETAEIIFINTCAIRDNAE 67
           +++P+ GR+YHETYGCQMNINDME+VLSIMK AGY E VD PE+AEIIFINTCAIRDNAE
Sbjct: 20  TEIPVRGRIYHETYGCQMNINDMEIVLSIMKDAGYREVVDVPESAEIIFINTCAIRDNAE 79

Query: 68  QKVWQRLNYFWFLKRNWKSNVATGRSQSRHPPKVVVLGCMAERLKDKILDSDKMVDVVCG 127
           QKVWQRLNYFWFLKR+WKSNV  GRSQS  PPKVVVLGCMAERLKDKILD+DKMVDVVCG
Sbjct: 80  QKVWQRLNYFWFLKRHWKSNVTIGRSQSLSPPKVVVLGCMAERLKDKILDADKMVDVVCG 139

Query: 128 PDAYRDLPRLLDEVDYGQKGINTLLSLEETYADISPVRIAKNSVTAFVSVMRGCNNMCSF 187
           PDAYRDLPRLL+EVDYGQKGINTLLSLEETYADI PVRI+KNS+TAFVSVMRGCNNMCSF
Sbjct: 140 PDAYRDLPRLLEEVDYGQKGINTLLSLEETYADICPVRISKNSITAFVSVMRGCNNMCSF 199

Query: 188 CIVPFTRGRERSRPVESIVCEVRELYREGVKEVTLLGQNVNSYNDTT--ENADIEPGTNW 247
           CIVPFTRGRERSRPV+SIV EV EL++EGVKEVTLLGQNVNSYND +  EN ++EPG+NW
Sbjct: 200 CIVPFTRGRERSRPVDSIVKEVAELWKEGVKEVTLLGQNVNSYNDASGVEN-EVEPGSNW 259

Query: 248 KLSDGFSTIAKVKKMGLRFSDLLDRLSTEFPEMRFRYTSPHPKDFPDEFLYLMRDRHNIC 307
           KLS+GFS++ KVKKMGLRFSDLLDRLSTEFPEMRFRYTSPHPKDFPD+ LY+MRD++NIC
Sbjct: 260 KLSEGFSSMCKVKKMGLRFSDLLDRLSTEFPEMRFRYTSPHPKDFPDDLLYVMRDKYNIC 319

Query: 308 KSIHLPAQSGNSIVLERMRRGYTREAYLDLVHKIRSIIPDVGITSDFICGFCGETEEEHA 367
           KSIHLPAQSG+S VLERMRRGYTREAYLDLV KIR I+PDVGITSDFICGFCGETEE+H 
Sbjct: 320 KSIHLPAQSGSSTVLERMRRGYTREAYLDLVQKIRRIMPDVGITSDFICGFCGETEEDHE 379

Query: 368 DTLSLINEVGYDMAYMFAYSMREKTHAHRNYVDDIPEEVKQRRLTELIEAFRNSTGRCYD 427
            TLSL+  VGYDMAYMFAYSMREKTHAHRNY DD+P+ VKQRRLTELIEAFR STG+CYD
Sbjct: 380 STLSLVRTVGYDMAYMFAYSMREKTHAHRNYNDDVPDNVKQRRLTELIEAFRGSTGQCYD 439

Query: 428 SQIGSVQLVLVEGPNKRAPETEMIGKSDRSHRVSFVNVAVPHRDGDYDGHRKAMVGDFVE 487
           SQIGS+QLVLVEGPNKRAP+TE+IGK+DR HRVSF+N+AVP+R   +   R  +VGD+VE
Sbjct: 440 SQIGSIQLVLVEGPNKRAPDTELIGKTDRGHRVSFINLAVPNRSEGFINKRNPVVGDYVE 499

Query: 488 VRISKSTRASLFGEALAITKLSSFYNGMD-EPIAC 520
           VRI KSTRASLFGEALAITKLS F++ +D E +AC
Sbjct: 500 VRILKSTRASLFGEALAITKLSLFHSNVDQEAVAC 533

BLAST of Cucsa.184640 vs. TAIR10
Match: AT4G36390.1 (AT4G36390.1 Methylthiotransferase)

HSP 1 Score: 823.5 bits (2126), Expect = 7.1e-239
Identity = 403/508 (79.33%), Postives = 449/508 (88.39%), Query Frame = 1

Query: 8   SDVPLSGRVYHETYGCQMNINDMEVVLSIMKKAGYSETVDTPETAEIIFINTCAIRDNAE 67
           SD+   GR+YHETYGCQMNINDME+VL+IMK +GY E V  PE+AE+IF+NTCAIR+NAE
Sbjct: 123 SDIASKGRIYHETYGCQMNINDMEIVLAIMKNSGYKEVVTDPESAEVIFVNTCAIRENAE 182

Query: 68  QKVWQRLNYFWFLKRNWKSNVATGRSQSRHPPKVVVLGCMAERLKDKILDSDKMVDVVCG 127
           Q+VWQRLNYFWFLKR WK N ATGR++S  PPKVVVLGCMAERLKDKILDSDKMVDVVCG
Sbjct: 183 QRVWQRLNYFWFLKREWKVNAATGRAKSLKPPKVVVLGCMAERLKDKILDSDKMVDVVCG 242

Query: 128 PDAYRDLPRLLDEVDYGQKGINTLLSLEETYADISPVRIAKNSVTAFVSVMRGCNNMCSF 187
           PDAYRDLPRLL+EVDYGQKGINTLLSLEETYADISPVRI++NS+TAFVSVMRGCNNMC+F
Sbjct: 243 PDAYRDLPRLLEEVDYGQKGINTLLSLEETYADISPVRISENSITAFVSVMRGCNNMCAF 302

Query: 188 CIVPFTRGRERSRPVESIVCEVRELYREGVKEVTLLGQNVNSYNDTTENADIEPGTNWKL 247
           CIVPFTRGRERSRPVESI+ EV EL+  GVKEVTLLGQNVNSYND  ++AD E G NW+ 
Sbjct: 303 CIVPFTRGRERSRPVESIIREVGELWESGVKEVTLLGQNVNSYND--DSADRESGANWEY 362

Query: 248 SDGFSTIAKVKKMGLRFSDLLDRLSTEFPEMRFRYTSPHPKDFPDEFLYLMRDRHNICKS 307
           S+GFS+  KVK MGLRF+DLLDRLS EFPEMRFR+TSPHPKD+PDE LYLMRDRHNIC  
Sbjct: 363 SEGFSSRCKVKNMGLRFADLLDRLSVEFPEMRFRFTSPHPKDYPDELLYLMRDRHNICNL 422

Query: 308 IHLPAQSGNSIVLERMRRGYTREAYLDLVHKIRSIIPDVGITSDFICGFCGETEEEHADT 367
           IHLPAQSGNS +LE+MRRGYTREAYLDLV KIRSIIPDV ITSDFI GFCGETEEEH +T
Sbjct: 423 IHLPAQSGNSRILEQMRRGYTREAYLDLVKKIRSIIPDVAITSDFITGFCGETEEEHQET 482

Query: 368 LSLINEVGYDMAYMFAYSMREKTHAHRNYVDDIPEEVKQRRLTELIEAFRNSTGRCYDSQ 427
           LSL+  VGYDMAYMFAYSMREKTHAHRNY DD+PEEVKQRRLTELI+AFR +TG CYDSQ
Sbjct: 483 LSLVRAVGYDMAYMFAYSMREKTHAHRNYTDDVPEEVKQRRLTELIDAFRETTGPCYDSQ 542

Query: 428 IGSVQLVLVEGPNKRAPETEMIGKSDRSHRVSFVNVAVPHR----DGDYDGHRKAMVGDF 487
           +GS QLVLVEGPNKRAPETE+IGK+D+ HRVSFV   +  +    DGD D  R   +GDF
Sbjct: 543 VGSTQLVLVEGPNKRAPETELIGKTDKGHRVSFVTKPLFDKACLLDGD-DLKRNPGIGDF 602

Query: 488 VEVRISKSTRASLFGEALAITKLSSFYN 512
           VEV+I KSTRASLFGEALAI+K+S F++
Sbjct: 603 VEVQIEKSTRASLFGEALAISKMSLFHD 627

BLAST of Cucsa.184640 vs. TAIR10
Match: AT1G72090.1 (AT1G72090.1 Methylthiotransferase)

HSP 1 Score: 136.3 bits (342), Expect = 5.2e-32
Identity = 124/510 (24.31%), Postives = 220/510 (43.14%), Query Frame = 1

Query: 6   PASDVPLSGRVYHETYGCQMNINDMEVVLSIMKKAGYSETVDTPETAEIIFINTCAIRDN 65
           P+  +P +  +Y +T+GC  N +D E +   +   GY+ T + PE A++  INTC ++  
Sbjct: 56  PSMKIPGTQTIYIKTFGCSHNQSDSEYMAGQLSAFGYALT-EVPEEADLWLINTCTVKSP 115

Query: 66  AEQKVWQRLNYFWFLKRNWKSNVATGRSQSRHPPKVVVLGCMAERLKD-KILDSDKMVDV 125
           ++  +               + +  GRS  +    +V+ GC+ +  +D K L+   +V V
Sbjct: 116 SQSAM--------------STLITRGRSGKK---PLVIAGCVPQGSRDLKELEGVSVVGV 175

Query: 126 VCGPDAYRDLPRLLDEVDYGQKGINTLLSLEETYADISPVRIAKNSVTAFVSVMRGCNNM 185
                  + + R+++ V+   KG    L   +T   +   ++ +N+    + +  GC   
Sbjct: 176 -------QQIDRVVEIVEETLKGHEVRLLTRKTLPALDLPKVRRNNFIEILPINVGCLGA 235

Query: 186 CSFCIVPFTRGRERSRPVESIVCEVRELYREGVKEVTLLGQNVNSYNDTTENADIEPGTN 245
           C++C     RG   S  V+S+V  VR +  EGVKE+ L  ++  +Y              
Sbjct: 236 CTYCKTKHARGHLGSYTVDSLVERVRTVISEGVKEIWLSSEDTGAYG------------- 295

Query: 246 WKLSDGFSTIAKVKKMGLRFSDLLDRLSTEFPE-----MRFRYTSP-----HPKDFPDEF 305
                        + +G+    LL+ +  E P      +R   T+P     H K+     
Sbjct: 296 -------------RDIGVNLPILLNAIVKELPSDQSTMLRIGMTNPPFILEHLKEIAAVL 355

Query: 306 LYLMRDRHN-ICKSIHLPAQSGNSIVLERMRRGYTREAYLDLVHKIRSIIPDVGITSDFI 365
                 RH  +   +H+P QSG+  VL  M R YT   +  +V  +  ++P + I +D I
Sbjct: 356 ------RHPCVYTFLHVPVQSGSDSVLTAMNREYTASEFRTVVDTLTELVPGMQIATDII 415

Query: 366 CGFCGETEEEHADTLSLINEVGYDMAYMFAYSMREKTHAHR-NYVDDIPEEVKQRRLTEL 425
           CGF GET+E+ + T+ LI +  +   ++  +  R  T A +   V     + + R LT +
Sbjct: 416 CGFPGETDEDFSQTVELIKDYKFPQVHISQFYPRPGTPAAKMKKVQSKIVKQRSRELTSV 475

Query: 426 IEAFRNSTG-RCYDSQIGSVQLVLVEGPNKRAPETEMIGKSDRSHRVSFVNVAVPHRDGD 485
            EAF   TG  C + +I   + V  +G         ++G     H   +V V V   +  
Sbjct: 476 FEAFAPYTGMECREERIWITE-VATDG-------IHLVG-----HTKGYVQVLVTGPE-- 487

Query: 486 YDGHRKAMVGDFVEVRISKSTRASLFGEAL 502
                 +M+G     RI+   R S+FGE +
Sbjct: 536 ------SMLGTSAMARITSVGRWSVFGEVI 487

BLAST of Cucsa.184640 vs. NCBI nr
Match: gi|449444679|ref|XP_004140101.1| (PREDICTED: CDK5RAP1-like protein [Cucumis sativus])

HSP 1 Score: 1053.5 bits (2723), Expect = 1.2e-304
Identity = 519/520 (99.81%), Postives = 519/520 (99.81%), Query Frame = 1

Query: 2   TRSLPASDVPLSGRVYHETYGCQMNINDMEVVLSIMKKAGYSETVDTPETAEIIFINTCA 61
           T SLPASDVPLSGRVYHETYGCQMNINDMEVVLSIMKKAGYSETVDTPETAEIIFINTCA
Sbjct: 116 TESLPASDVPLSGRVYHETYGCQMNINDMEVVLSIMKKAGYSETVDTPETAEIIFINTCA 175

Query: 62  IRDNAEQKVWQRLNYFWFLKRNWKSNVATGRSQSRHPPKVVVLGCMAERLKDKILDSDKM 121
           IRDNAEQKVWQRLNYFWFLKRNWKSNVATGRSQSRHPPKVVVLGCMAERLKDKILDSDKM
Sbjct: 176 IRDNAEQKVWQRLNYFWFLKRNWKSNVATGRSQSRHPPKVVVLGCMAERLKDKILDSDKM 235

Query: 122 VDVVCGPDAYRDLPRLLDEVDYGQKGINTLLSLEETYADISPVRIAKNSVTAFVSVMRGC 181
           VDVVCGPDAYRDLPRLLDEVDYGQKGINTLLSLEETYADISPVRIAKNSVTAFVSVMRGC
Sbjct: 236 VDVVCGPDAYRDLPRLLDEVDYGQKGINTLLSLEETYADISPVRIAKNSVTAFVSVMRGC 295

Query: 182 NNMCSFCIVPFTRGRERSRPVESIVCEVRELYREGVKEVTLLGQNVNSYNDTTENADIEP 241
           NNMCSFCIVPFTRGRERSRPVESIVCEVRELYREGVKEVTLLGQNVNSYNDTTENADIEP
Sbjct: 296 NNMCSFCIVPFTRGRERSRPVESIVCEVRELYREGVKEVTLLGQNVNSYNDTTENADIEP 355

Query: 242 GTNWKLSDGFSTIAKVKKMGLRFSDLLDRLSTEFPEMRFRYTSPHPKDFPDEFLYLMRDR 301
           GTNWKLSDGFSTIAKVKKMGLRFSDLLDRLSTEFPEMRFRYTSPHPKDFPDEFLYLMRDR
Sbjct: 356 GTNWKLSDGFSTIAKVKKMGLRFSDLLDRLSTEFPEMRFRYTSPHPKDFPDEFLYLMRDR 415

Query: 302 HNICKSIHLPAQSGNSIVLERMRRGYTREAYLDLVHKIRSIIPDVGITSDFICGFCGETE 361
           HNICKSIHLPAQSGNSIVLERMRRGYTREAYLDLVHKIRSIIPDVGITSDFICGFCGETE
Sbjct: 416 HNICKSIHLPAQSGNSIVLERMRRGYTREAYLDLVHKIRSIIPDVGITSDFICGFCGETE 475

Query: 362 EEHADTLSLINEVGYDMAYMFAYSMREKTHAHRNYVDDIPEEVKQRRLTELIEAFRNSTG 421
           EEHADTLSLINEVGYDMAYMFAYSMREKTHAHRNYVDDIPEEVKQRRLTELIEAFRNSTG
Sbjct: 476 EEHADTLSLINEVGYDMAYMFAYSMREKTHAHRNYVDDIPEEVKQRRLTELIEAFRNSTG 535

Query: 422 RCYDSQIGSVQLVLVEGPNKRAPETEMIGKSDRSHRVSFVNVAVPHRDGDYDGHRKAMVG 481
           RCYDSQIGSVQLVLVEGPNKRAPETEMIGKSDRSHRVSFVNVAVPHRDGDYDGHRKAMVG
Sbjct: 536 RCYDSQIGSVQLVLVEGPNKRAPETEMIGKSDRSHRVSFVNVAVPHRDGDYDGHRKAMVG 595

Query: 482 DFVEVRISKSTRASLFGEALAITKLSSFYNGMDEPIACMS 522
           DFVEVRISKSTRASLFGEALAITKLSSFYNGMDEPIACMS
Sbjct: 596 DFVEVRISKSTRASLFGEALAITKLSSFYNGMDEPIACMS 635

BLAST of Cucsa.184640 vs. NCBI nr
Match: gi|659097074|ref|XP_008449427.1| (PREDICTED: CDK5RAP1-like protein [Cucumis melo])

HSP 1 Score: 1032.7 bits (2669), Expect = 2.2e-298
Identity = 509/520 (97.88%), Postives = 512/520 (98.46%), Query Frame = 1

Query: 2   TRSLPASDVPLSGRVYHETYGCQMNINDMEVVLSIMKKAGYSETVDTPETAEIIFINTCA 61
           T SLPASDVP  GRVYHETYGCQMNINDMEVVLSIMKKAGY ETVDTPETAEIIFINTCA
Sbjct: 113 TESLPASDVPSRGRVYHETYGCQMNINDMEVVLSIMKKAGYGETVDTPETAEIIFINTCA 172

Query: 62  IRDNAEQKVWQRLNYFWFLKRNWKSNVATGRSQSRHPPKVVVLGCMAERLKDKILDSDKM 121
           IRDNAEQKVWQRLNYFWFLKR WKSNVA GRSQSRHPPKVVVLGCMAERLKDKILDSDKM
Sbjct: 173 IRDNAEQKVWQRLNYFWFLKRQWKSNVAIGRSQSRHPPKVVVLGCMAERLKDKILDSDKM 232

Query: 122 VDVVCGPDAYRDLPRLLDEVDYGQKGINTLLSLEETYADISPVRIAKNSVTAFVSVMRGC 181
           VDVVCGPDAYRDLPRLLDEVDYGQKGINTLLSLEETYADISPVRIAKNSVTAFVSVMRGC
Sbjct: 233 VDVVCGPDAYRDLPRLLDEVDYGQKGINTLLSLEETYADISPVRIAKNSVTAFVSVMRGC 292

Query: 182 NNMCSFCIVPFTRGRERSRPVESIVCEVRELYREGVKEVTLLGQNVNSYNDTTENADIEP 241
           NNMCSFCIVPFTRGRERSRPVESIVCEV+ELYREGVKEVTLLGQNVNSYNDTTENADIEP
Sbjct: 293 NNMCSFCIVPFTRGRERSRPVESIVCEVKELYREGVKEVTLLGQNVNSYNDTTENADIEP 352

Query: 242 GTNWKLSDGFSTIAKVKKMGLRFSDLLDRLSTEFPEMRFRYTSPHPKDFPDEFLYLMRDR 301
           GTNWKLSDGFSTIAKVKKMGLRFSDLLDRLSTEFPEMRFRYTSPHPKDFPDEFLYLMRDR
Sbjct: 353 GTNWKLSDGFSTIAKVKKMGLRFSDLLDRLSTEFPEMRFRYTSPHPKDFPDEFLYLMRDR 412

Query: 302 HNICKSIHLPAQSGNSIVLERMRRGYTREAYLDLVHKIRSIIPDVGITSDFICGFCGETE 361
           HNICKSIHLPAQSGNSIVLERMRRGYTREAYLDLVHKIRSIIPDVGITSDFICGFCGETE
Sbjct: 413 HNICKSIHLPAQSGNSIVLERMRRGYTREAYLDLVHKIRSIIPDVGITSDFICGFCGETE 472

Query: 362 EEHADTLSLINEVGYDMAYMFAYSMREKTHAHRNYVDDIPEEVKQRRLTELIEAFRNSTG 421
           EEHADTLSLINEVGYDMAYMFAYSMREKTHAHRNYVDDIPEEVKQRRLTELIEAFRNSTG
Sbjct: 473 EEHADTLSLINEVGYDMAYMFAYSMREKTHAHRNYVDDIPEEVKQRRLTELIEAFRNSTG 532

Query: 422 RCYDSQIGSVQLVLVEGPNKRAPETEMIGKSDRSHRVSFVNVAVPHRDGDYDGHRKAMVG 481
           RCYDSQIGSVQLVLVEGPNKRAPETEMIGKSDRSHRVSFVNVAVPHRDGDYDGHRKA+VG
Sbjct: 533 RCYDSQIGSVQLVLVEGPNKRAPETEMIGKSDRSHRVSFVNVAVPHRDGDYDGHRKAVVG 592

Query: 482 DFVEVRISKSTRASLFGEALAITKLSSFYNGMDEPIACMS 522
           DFVEVRISKSTRASLFGEALAITKLSSFYN MDE IAC+S
Sbjct: 593 DFVEVRISKSTRASLFGEALAITKLSSFYNRMDESIACVS 632

BLAST of Cucsa.184640 vs. NCBI nr
Match: gi|1009144577|ref|XP_015889876.1| (PREDICTED: CDK5RAP1-like protein [Ziziphus jujuba])

HSP 1 Score: 899.0 bits (2322), Expect = 3.8e-258
Identity = 440/518 (84.94%), Postives = 481/518 (92.86%), Query Frame = 1

Query: 5   LPASDVPLSGRVYHETYGCQMNINDMEVVLSIMKKAGYSETVDTPETAEIIFINTCAIRD 64
           LPAS++   GR+YHETYGCQMNINDME+VLSIMKKAGYSE+VD PE+AEIIFINTCAIRD
Sbjct: 98  LPASEISSRGRIYHETYGCQMNINDMEIVLSIMKKAGYSESVDNPESAEIIFINTCAIRD 157

Query: 65  NAEQKVWQRLNYFWFLKRNWKSNVATGRSQSRHPPKVVVLGCMAERLKDKILDSDKMVDV 124
           NAEQKVWQRLNYFWFLKR+WKSNVA GRSQS  PPKVVVLGCMAERLKDKILD+DKMVDV
Sbjct: 158 NAEQKVWQRLNYFWFLKRHWKSNVAIGRSQSIRPPKVVVLGCMAERLKDKILDADKMVDV 217

Query: 125 VCGPDAYRDLPRLLDEVDYGQKGINTLLSLEETYADISPVRIAKNSVTAFVSVMRGCNNM 184
           VCGPDAYRDLPRLL+EVDYGQKGINTLLSLEETYADISPVRI+KNSVTAFVSVMRGCNNM
Sbjct: 218 VCGPDAYRDLPRLLEEVDYGQKGINTLLSLEETYADISPVRISKNSVTAFVSVMRGCNNM 277

Query: 185 CSFCIVPFTRGRERSRPVESIVCEVRELYREGVKEVTLLGQNVNSYNDTTE-NADIEPGT 244
           CSFCIVPFTRGRERSRPV+SIV EV EL++EGVKEVTLLGQNVNSYNDT+    ++EPGT
Sbjct: 278 CSFCIVPFTRGRERSRPVDSIVREVAELWKEGVKEVTLLGQNVNSYNDTSRVENEVEPGT 337

Query: 245 NWKLSDGFSTIAKVKKMGLRFSDLLDRLSTEFPEMRFRYTSPHPKDFPDEFLYLMRDRHN 304
           NW+LS+GFS+++KVKKMGLRFSDLLDRLS EFP MRFRYTSPHPKDFPDEFLYLMRDRHN
Sbjct: 338 NWRLSEGFSSMSKVKKMGLRFSDLLDRLSREFPGMRFRYTSPHPKDFPDEFLYLMRDRHN 397

Query: 305 ICKSIHLPAQSGNSIVLERMRRGYTREAYLDLVHKIRSIIPDVGITSDFICGFCGETEEE 364
           IC+ IHLPAQSG++ VLERMRRGYTREAYLDLV KIR IIPDVGI+SDFICGFCGETEEE
Sbjct: 398 ICRYIHLPAQSGSTNVLERMRRGYTREAYLDLVQKIRRIIPDVGISSDFICGFCGETEEE 457

Query: 365 HADTLSLINEVGYDMAYMFAYSMREKTHAHRNYVDDIPEEVKQRRLTELIEAFRNSTGRC 424
           HADTLSLI  VGYDMAYMFAYSMREKTHA+RNY DD+PEEVKQRRLTELIEAFR STG+C
Sbjct: 458 HADTLSLIRAVGYDMAYMFAYSMREKTHANRNYSDDVPEEVKQRRLTELIEAFRQSTGQC 517

Query: 425 YDSQIGSVQLVLVEGPNKRAPETEMIGKSDRSHRVSFVNVAVPHRDGDYDGHRKAMVGDF 484
           +DSQ+G+VQLVLVEGPNKRAP+TE+IGKSDR HRVSFV+VAV  RD D +  R  +VGD+
Sbjct: 518 FDSQVGTVQLVLVEGPNKRAPDTELIGKSDRGHRVSFVSVAVQDRDSDPNAKRNPVVGDY 577

Query: 485 VEVRISKSTRASLFGEALAITKLSSFYNGMDEPIACMS 522
           VEVRI KSTRASLFGEALAIT+LSSFYN  ++ +AC S
Sbjct: 578 VEVRILKSTRASLFGEALAITRLSSFYNVHEDAVACGS 615

BLAST of Cucsa.184640 vs. NCBI nr
Match: gi|719996007|ref|XP_010254686.1| (PREDICTED: CDK5RAP1-like protein [Nelumbo nucifera])

HSP 1 Score: 886.3 bits (2289), Expect = 2.5e-254
Identity = 436/518 (84.17%), Postives = 475/518 (91.70%), Query Frame = 1

Query: 4   SLPASDVPLSGRVYHETYGCQMNINDMEVVLSIMKKAGYSETVDTPETAEIIFINTCAIR 63
           SLPAS+V   GR+YHETYGCQMNINDME+VLS+MK AGY E VD PE+AEIIFINTCAIR
Sbjct: 100 SLPASEVHPRGRIYHETYGCQMNINDMEIVLSVMKNAGYGEVVDVPESAEIIFINTCAIR 159

Query: 64  DNAEQKVWQRLNYFWFLKRNWKSNVATGRSQSRHPPKVVVLGCMAERLKDKILDSDKMVD 123
           DNAEQKVWQRLNYFWFLKR+WKSNV+ GRSQS HPPKVVVLGCMAERLKDKILD+DKMVD
Sbjct: 160 DNAEQKVWQRLNYFWFLKRHWKSNVSIGRSQSWHPPKVVVLGCMAERLKDKILDADKMVD 219

Query: 124 VVCGPDAYRDLPRLLDEVDYGQKGINTLLSLEETYADISPVRIAKNSVTAFVSVMRGCNN 183
           VVCGPDAYRDLPRLL+EVD GQKGINTLLSLEETYADISPVRI+KNSVTAFVSVMRGCNN
Sbjct: 220 VVCGPDAYRDLPRLLEEVDDGQKGINTLLSLEETYADISPVRISKNSVTAFVSVMRGCNN 279

Query: 184 MCSFCIVPFTRGRERSRPVESIVCEVRELYREGVKEVTLLGQNVNSYNDTTE-NADIEPG 243
           MCSFCIVPFTRGRERSRPVESIV EV EL++EGVKEV LLGQNVNSYND +  + D+EPG
Sbjct: 280 MCSFCIVPFTRGRERSRPVESIVREVGELWKEGVKEVMLLGQNVNSYNDASGVDKDVEPG 339

Query: 244 TNWKLSDGFSTIAKVKKMGLRFSDLLDRLSTEFPEMRFRYTSPHPKDFPDEFLYLMRDRH 303
            NWK S+GFS++ KVK MGLRF+DLLDRLSTEFPEMRFRYTSPHPKDFPDE LYLMRDR+
Sbjct: 340 INWKYSEGFSSMCKVKNMGLRFADLLDRLSTEFPEMRFRYTSPHPKDFPDELLYLMRDRY 399

Query: 304 NICKSIHLPAQSGNSIVLERMRRGYTREAYLDLVHKIRSIIPDVGITSDFICGFCGETEE 363
           NICKSIHLPAQ+G++ VLERMRRGY+REAYLDLV KIRS IPDVGI+SDFICGFCGETEE
Sbjct: 400 NICKSIHLPAQTGSTTVLERMRRGYSREAYLDLVQKIRSTIPDVGISSDFICGFCGETEE 459

Query: 364 EHADTLSLINEVGYDMAYMFAYSMREKTHAHRNYVDDIPEEVKQRRLTELIEAFRNSTGR 423
           EHADTLSLI  VGYDMAYMFAYSMRE+THAHRNYVDD+PE+VKQRRLTELIEAFR++TG+
Sbjct: 460 EHADTLSLIRAVGYDMAYMFAYSMRERTHAHRNYVDDVPEDVKQRRLTELIEAFRDTTGK 519

Query: 424 CYDSQIGSVQLVLVEGPNKRAPETEMIGKSDRSHRVSFVNVAVPHRDGDYDGHRKAMVGD 483
           CYDSQIG++QLVLVEGPNKRAPE E+IGKSDR HRVSFVNVAVPHRD + D  R   VGD
Sbjct: 520 CYDSQIGTIQLVLVEGPNKRAPERELIGKSDRGHRVSFVNVAVPHRD-ETDEKRNPRVGD 579

Query: 484 FVEVRISKSTRASLFGEALAITKLSSFYNGMDE-PIAC 520
           +VEV I KST+ASLFGEALAIT LSSFY  MD+  +AC
Sbjct: 580 YVEVHILKSTKASLFGEALAITDLSSFYTKMDDAAVAC 616

BLAST of Cucsa.184640 vs. NCBI nr
Match: gi|224094881|ref|XP_002310277.1| (hypothetical protein POPTR_0007s13510g [Populus trichocarpa])

HSP 1 Score: 880.6 bits (2274), Expect = 1.4e-252
Identity = 431/525 (82.10%), Postives = 474/525 (90.29%), Query Frame = 1

Query: 5   LPASDVPLS------GRVYHETYGCQMNINDMEVVLSIMKKAGYSETVDTPETAEIIFIN 64
           +PA +VP        GR+YHETYGCQMN+NDME+VLSIMK AGYSE VD PE+AEIIFIN
Sbjct: 99  VPAIEVPPDIEILPRGRIYHETYGCQMNVNDMEIVLSIMKNAGYSEIVDVPESAEIIFIN 158

Query: 65  TCAIRDNAEQKVWQRLNYFWFLKRNWKSNVATGRSQSRHPPKVVVLGCMAERLKDKILDS 124
           TCAIRDNAEQKVWQRLNYFWFLKR+WKSNVA GRSQS HPPKVVVLGCMAERLKDKILD+
Sbjct: 159 TCAIRDNAEQKVWQRLNYFWFLKRHWKSNVAIGRSQSHHPPKVVVLGCMAERLKDKILDA 218

Query: 125 DKMVDVVCGPDAYRDLPRLLDEVDYGQKGINTLLSLEETYADISPVRIAKNSVTAFVSVM 184
           DKMVDVVCGPDAYRDLPRLL+EV+YGQKGINTLLSLEETYADISPVRI+KNS+ AFVSVM
Sbjct: 219 DKMVDVVCGPDAYRDLPRLLEEVEYGQKGINTLLSLEETYADISPVRISKNSINAFVSVM 278

Query: 185 RGCNNMCSFCIVPFTRGRERSRPVESIVCEVRELYREGVKEVTLLGQNVNSYNDTTE-NA 244
           RGCNNMCSFCIVPFTRGRERSRPVESIV EV EL++EGVKEVTLLGQNVNSYND +E   
Sbjct: 279 RGCNNMCSFCIVPFTRGRERSRPVESIVKEVAELWKEGVKEVTLLGQNVNSYNDASEIEK 338

Query: 245 DIEPGTNWKLSDGFSTIAKVKKMGLRFSDLLDRLSTEFPEMRFRYTSPHPKDFPDEFLYL 304
           ++EPGTNWK SDGFS+  KVK++GLRFSDLLDRLSTEFPEMRFRYTSPHPKDFPD+ LY+
Sbjct: 339 EVEPGTNWKYSDGFSSTCKVKRVGLRFSDLLDRLSTEFPEMRFRYTSPHPKDFPDDLLYV 398

Query: 305 MRDRHNICKSIHLPAQSGNSIVLERMRRGYTREAYLDLVHKIRSIIPDVGITSDFICGFC 364
           MR+RHNICKSIHLPAQ+G+S VLERMRRGYTREAYLDLV KIR IIPDVGITSDFICGFC
Sbjct: 399 MRNRHNICKSIHLPAQTGSSTVLERMRRGYTREAYLDLVQKIRRIIPDVGITSDFICGFC 458

Query: 365 GETEEEHADTLSLINEVGYDMAYMFAYSMREKTHAHRNYVDDIPEEVKQRRLTELIEAFR 424
           GETEEEH DTLSL+  VGYDMAYMFAYSMREKTHAHRNYVDD+P+EVK+RRL ELIEAFR
Sbjct: 459 GETEEEHQDTLSLVKAVGYDMAYMFAYSMREKTHAHRNYVDDVPDEVKKRRLAELIEAFR 518

Query: 425 NSTGRCYDSQIGSVQLVLVEGPNKRAPETEMIGKSDRSHRVSFVNVAVPHRDGDYDGHRK 484
            STG+C+DSQIGSVQLVLVEGPNKRAP+TE+IGKSDR HRV F N+ +P+R+ D    R 
Sbjct: 519 ESTGQCFDSQIGSVQLVLVEGPNKRAPDTELIGKSDRGHRVLFTNLPLPNRNEDGSQARN 578

Query: 485 AMVGDFVEVRISKSTRASLFGEALAITKLSSFYNGMDE-PIACMS 522
            MVGD+VEV I KSTRASLFG+ALAIT LSSFY+ +D+  +AC S
Sbjct: 579 PMVGDYVEVLILKSTRASLFGDALAITTLSSFYSNLDQKAVACAS 623

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
CK5P1_ARATH1.3e-23779.33CDK5RAP1-like protein OS=Arabidopsis thaliana GN=At4g36390 PE=2 SV=1[more]
CK5P1_ORYSJ2.4e-23679.07CDK5RAP1-like protein OS=Oryza sativa subsp. japonica GN=Os11g0592800 PE=2 SV=1[more]
CK5P1_RAT8.1e-14454.97CDK5 regulatory subunit-associated protein 1 OS=Rattus norvegicus GN=Cdk5rap1 PE... [more]
CK5P1_MOUSE1.8e-14354.56CDK5 regulatory subunit-associated protein 1 OS=Mus musculus GN=Cdk5rap1 PE=2 SV... [more]
CK5P1_DROME1.3e-14152.01CDK5RAP1-like protein OS=Drosophila melanogaster GN=CG6345 PE=2 SV=1[more]
Match NameE-valueIdentityDescription
A0A0A0KDY2_CUCSA8.4e-30599.81Uncharacterized protein OS=Cucumis sativus GN=Csa_6G411190 PE=4 SV=1[more]
B9HFB0_POPTR9.7e-25382.10Uncharacterized protein OS=Populus trichocarpa GN=POPTR_0007s13510g PE=4 SV=1[more]
M5WF79_PRUPE1.3e-25282.36Uncharacterized protein OS=Prunus persica GN=PRUPE_ppa002826mg PE=4 SV=1[more]
A5AL29_VITVI1.7e-25282.85Putative uncharacterized protein OS=Vitis vinifera GN=VITISV_007911 PE=4 SV=1[more]
B9S686_RICCO1.1e-25182.72Radical sam protein, putative OS=Ricinus communis GN=RCOM_0533210 PE=4 SV=1[more]
Match NameE-valueIdentityDescription
AT4G36390.17.1e-23979.33 Methylthiotransferase[more]
AT1G72090.15.2e-3224.31 Methylthiotransferase[more]
Match NameE-valueIdentityDescription
gi|449444679|ref|XP_004140101.1|1.2e-30499.81PREDICTED: CDK5RAP1-like protein [Cucumis sativus][more]
gi|659097074|ref|XP_008449427.1|2.2e-29897.88PREDICTED: CDK5RAP1-like protein [Cucumis melo][more]
gi|1009144577|ref|XP_015889876.1|3.8e-25884.94PREDICTED: CDK5RAP1-like protein [Ziziphus jujuba][more]
gi|719996007|ref|XP_010254686.1|2.5e-25484.17PREDICTED: CDK5RAP1-like protein [Nelumbo nucifera][more]
gi|224094881|ref|XP_002310277.1|1.4e-25282.10hypothetical protein POPTR_0007s13510g [Populus trichocarpa][more]
The following terms have been associated with this gene:
Vocabulary: INTERPRO
TermDefinition
IPR002792TRAM_dom
IPR005839Methylthiotransferase
IPR006638Elp3/MiaB/NifB
IPR007197rSAM
IPR013848Methylthiotransferase_N
IPR020612Methylthiotransferase_CS
IPR023404rSAM_horseshoe
IPR023970Methylthiotransferase/radical SAM-type protein
Vocabulary: Molecular Function
TermDefinition
GO:00515394 iron, 4 sulfur cluster binding
GO:0016740transferase activity
GO:0003824catalytic activity
GO:0051536iron-sulfur cluster binding
Vocabulary: Biological Process
TermDefinition
GO:0043412macromolecule modification
GO:0009451RNA modification
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0009073 aromatic amino acid family biosynthetic process
biological_process GO:0007131 reciprocal meiotic recombination
biological_process GO:0043412 macromolecule modification
biological_process GO:0009451 RNA modification
biological_process GO:0009691 cytokinin biosynthetic process
biological_process GO:0000079 regulation of cyclin-dependent protein serine/threonine kinase activity
biological_process GO:0010155 regulation of proton transport
biological_process GO:0046777 protein autophosphorylation
biological_process GO:0006655 phosphatidylglycerol biosynthetic process
biological_process GO:0055114 oxidation-reduction process
biological_process GO:0016226 iron-sulfur cluster assembly
biological_process GO:0009793 embryo development ending in seed dormancy
cellular_component GO:0005575 cellular_component
molecular_function GO:0032440 2-alkenal reductase [NAD(P)] activity
molecular_function GO:0051539 4 iron, 4 sulfur cluster binding
molecular_function GO:0016740 transferase activity
molecular_function GO:0003824 catalytic activity
molecular_function GO:0051536 iron-sulfur cluster binding

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Cucsa.184640.1Cucsa.184640.1mRNA


Analysis Name: InterPro Annotations of cucumber (Gy14)
Date Performed: 2017-01-17
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR002792TRAM domainPFAMPF01938TRAMcoord: 425..501
score: 4.3
IPR002792TRAM domainPROFILEPS50926TRAMcoord: 425..502
score: 14
IPR005839MethylthiotransferaseTIGRFAMsTIGR00089TIGR00089coord: 15..498
score: 7.5E
IPR006638Elongator protein 3/MiaB/NifBSMARTSM00729MiaBcoord: 171..411
score: 9.6
IPR007197Radical SAMPFAMPF04055Radical_SAMcoord: 175..371
score: 9.1
IPR013848Methylthiotransferase, N-terminalPFAMPF00919UPF0004coord: 15..127
score: 9.0
IPR013848Methylthiotransferase, N-terminalPROFILEPS51449MTTASE_Ncoord: 14..143
score: 37
IPR020612Methylthiotransferase, conserved sitePROSITEPS01278MTTASE_RADICALcoord: 175..195
scor
IPR023404Radical SAM, alpha/beta horseshoeGENE3DG3DSA:3.80.30.20coord: 170..421
score: 9.1
IPR023970Methylthiotransferase/radical SAM-type proteinPANTHERPTHR11918RADICAL SAM PROTEINScoord: 253..516
score: 5.3E-256coord: 3..233
score: 5.3E
NoneNo IPR availablePANTHERPTHR11918:SF43CDK5 REGULATORY SUBUNIT-ASSOCIATED PROTEIN 1coord: 3..233
score: 5.3E-256coord: 253..516
score: 5.3E
NoneNo IPR availableTIGRFAMsTIGR01574TIGR01574coord: 16..499
score: 1.1E
NoneNo IPR availableunknownSSF102114Radical SAM enzymescoord: 165..233
score: 4.32E-53coord: 263..418
score: 4.32

The following gene(s) are paralogous to this gene:

None

The following block(s) are covering this gene:
GeneOrganismBlock
Cucsa.184640Bottle gourd (USVL1VR-Ls)cgylsiB310
Cucsa.184640Watermelon (97103) v2cgywmbB341
Cucsa.184640Wax gourdcgywgoB377
Cucsa.184640Watermelon (Charleston Gray)cgywcgB329