Cucsa.181130 (gene) Cucumber (Gy14) v1 | Cucurbit Genomics Database (CuGenDB)

Overview


Name	Cucsa.181130
Type	gene
Organism	Cucumis sativus (Cucumber (Gy14) v1)
Description	Pectin lyase-like superfamily protein
Location	scaffold01239 : 135981 .. 137770 (-)

Genome Viewer

Gene Expression

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDS

Hold the cursor over a type above to highlight its positions in the sequence below.

GCTTTGAAAGATGCATGGAATGACGCATGTGCATCTGATCGTCCAAGTGCAGTGGTTATTCCAAAAGGAACATTTAAAGTAAGAGAAGGAGAATTTAAAGGCCCTTGCAAGAGCCCTATTGAATTTCGAGTTCATGGAACATTGCAGGCTCCAAAACACCCTCATGGAGATAGTTGGATTACTTTTGCATATGTTGATAGATTGAGACTATCAGGTGGAGGAGTTTTTGATGGCCAAGGAAAGGCAGGTTGGGAAAAGAATGATTGCCATAAACGTATAGATTGTGCTAAACTCCCAATTGTAAGTTCTATATCTTTTGTTTTCCTATCCATTAACTACATTCGTCTTTGTTCATTATGAAGAGGTTTGTTAGATTTTCGTTCTCTATTATTTTCATTTTTACAATTTGGTAAATGTGTTCTTAATACCATACGATAATGATTAAGAAATTAAATATTGAACGAAACTTAACCATAAAAAATTAGGCAAAAGAGCCATTTTTTTATATAAAAATATAAAGACTAAATAGATAGAGAAAACTCATAATTTTCATCCCTCCCTAATAGTTTGTGCATGACTTTCTCCCCCATCTCTGACACNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNATCTCAATCTTGAAATCTCGAATAGCAACAGGAGATGATTGCATCTCACTTGGAGATGGAAGTAAACGAATAAAAGTCAATAATGTGATATGTGGGCCAGGGCATGGAATAAGCATAGGAAGTCTTGGAAAATACAATAACGAAGAACCTGTTGAAGGTGTCATAGTAAATAATTGCACAATAATAAACACCACCAATGGTGTTAGGATCAAAACATGGCCAGCCTCTCCCGTTGCTGGTATTGCCACCAACATGCATTTTTCAGACATTACAATGGTCAACGTTAGCAACCCAATTCTCATAGACCAGGAGTACTGTCCATGGAATCAGTGCAATCGTGAGGTATTGATCACATTCTTAAAACTTTGATTTTGAAATTCCTTCATACATTTCAACTTCACGAATAAACTTTGATTTTCACTTTCAATTTTTCAGATTCCGTCAAATATTAAGATTAATAAGGTGAGCTTCAAGAACATTAGAGGAAGTTCTGCAACTCCAGTGGCAGTCAAACTTATTTGTAGTAGCAACCTACCATGTGAAGAAGTAAAAGTAGCCAACATTGACCTCGTTTATAATGGAATAAAAGGTTCTATTACTTCTGAATGCATGAATGTGAAACCAATCATTTCAGGAATACAAAATCCTCCGATTTGCTCTTCTTCTTACACCACTGCTTCAAAATGA

mRNA sequence

GCTTTGAAAGATGCATGGAATGACGCATGTGCATCTGATCGTCCAAGTGCAGTGGTTATTCCAAaaggaacatttaaagtaagagaaggagaatttaaaggcccttgcaagagccctattgaatttcgagttcatggaacattgcaggctccaaaacaccctcatggagatagttggattacttttgcatatgttgatagattgagactatcaggtggaggagtttttgatggccaaggaaaggcaggttgggaaaagaatgattgccataaacgtatagattgtgctaaactcccaattgtaacaacaggagatgattgcatctcacttggagatggaagtaaacgaataaaagtcaataatgtgatatgtgggccagggcatggaataagcataggaagtcttggaaaatacaataacgaagaacctgttgaaggtgtcatagtaaataattgcacaataataaacaccaccaatggtgttaggatcaaaacatggccagcctctcccgttgctggtattgccaccaacatgcatttttcagacattacaatggtcaacgttagcaacccaattctcatagaccaggagtactgtccatggaatcagtgcaatcgtgagattccgtcaaatattaagattaataaggtgagcttcaagaacattagaggaagttctgcaactccagtggcagtcaaacttatttgtagtagcaacctaccatgtgaagaagtaaaagtagccaacattgacctcgtttataatggaataaaaggttctattacttctgaatgcatgaatgtgaaaccaatcatttcaggaatacaaaatcctccgatttgctcttcttcttacaccactgcttcaaaatga

Coding sequence (CDS)

GCTTTGAAAGATGCATGGAATGACGCATGTGCATCTGATCGTCCAAGTGCAGTGGTTATTCCAAAAGGAACATTTAAAGTAAGAGAAGGAGAATTTAAAGGCCCTTGCAAGAGCCCTATTGAATTTCGAGTTCATGGAACATTGCAGGCTCCAAAACACCCTCATGGAGATAGTTGGATTACTTTTGCATATGTTGATAGATTGAGACTATCAGGTGGAGGAGTTTTTGATGGCCAAGGAAAGGCAGGTTGGGAAAAGAATGATTGCCATAAACGTATAGATTGTGCTAAACTCCCAATTGTAACAACAGGAGATGATTGCATCTCACTTGGAGATGGAAGTAAACGAATAAAAGTCAATAATGTGATATGTGGGCCAGGGCATGGAATAAGCATAGGAAGTCTTGGAAAATACAATAACGAAGAACCTGTTGAAGGTGTCATAGTAAATAATTGCACAATAATAAACACCACCAATGGTGTTAGGATCAAAACATGGCCAGCCTCTCCCGTTGCTGGTATTGCCACCAACATGCATTTTTCAGACATTACAATGGTCAACGTTAGCAACCCAATTCTCATAGACCAGGAGTACTGTCCATGGAATCAGTGCAATCGTGAGATTCCGTCAAATATTAAGATTAATAAGGTGAGCTTCAAGAACATTAGAGGAAGTTCTGCAACTCCAGTGGCAGTCAAACTTATTTGTAGTAGCAACCTACCATGTGAAGAAGTAAAAGTAGCCAACATTGACCTCGTTTATAATGGAATAAAAGGTTCTATTACTTCTGAATGCATGAATGTGAAACCAATCATTTCAGGAATACAAAATCCTCCGATTTGCTCTTCTTCTTACACCACTGCTTCAAAATGA

Protein sequence

ALKDAWNDACASDRPSAVVIPKGTFKVREGEFKGPCKSPIEFRVHGTLQAPKHPHGDSWITFAYVDRLRLSGGGVFDGQGKAGWEKNDCHKRIDCAKLPIVTTGDDCISLGDGSKRIKVNNVICGPGHGISIGSLGKYNNEEPVEGVIVNNCTIINTTNGVRIKTWPASPVAGIATNMHFSDITMVNVSNPILIDQEYCPWNQCNREIPSNIKINKVSFKNIRGSSATPVAVKLICSSNLPCEEVKVANIDLVYNGIKGSITSECMNVKPIISGIQNPPICSSSYTTASK*

Homology

[Close]

BLAST of Cucsa.181130 vs. Swiss-Prot
Match: PGLR_OENOR (Exopolygalacturonase (Fragment) OS=Oenothera organensis PE=2 SV=1)

HSP 1 Score: 244.2 bits (622), Expect = 1.8e-63
Identity = 114/189 (60.32%), Postives = 140/189 (74.07%), Query Frame = 1


Query: 101 VTTGDDCISLGDGSKRIKVNNVICGPGHGISIGSLGKYNNEEPVEGVIVNNCTIINTTNG 160
           + TGDDCISLGDGSK I + N+ CGPGHGIS+GSLG+Y NEE V G+ V NCTI  + NG
Sbjct: 175 IKTGDDCISLGDGSKNINITNITCGPGHGISVGSLGRYKNEESVVGIYVKNCTITGSQNG 234

Query: 161 VRIKTWPASPVAGIATNMHFSDITMVNVSNPILIDQEYCPWNQCNREIPSNIKINKVSFK 220
           VRIKTWP S   G A+ MHF DITM +V  PILIDQ YCP+NQC  E+PS++K++K+SFK
Sbjct: 235 VRIKTWPKSE-PGEASEMHFQDITMNSVGTPILIDQGYCPYNQCTAEVPSSVKLSKISFK 294

Query: 221 NIRGSSATPVAVKLICSSNLPCEEVKVANIDLVYNGIKGSITSECMNVKPIISGIQNPPI 280
           NI+G+S T  AVKL+CS + PC  V++A+IDL Y+G  G  TS C N+KP I G Q P I
Sbjct: 295 NIKGTSTTKEAVKLVCSKSFPCNGVELADIDLTYSGKGGPATSVCENIKPTIKGKQIPAI 354

Query: 281 CSSSYTTAS 290
           CS S   A+
Sbjct: 355 CSGSAAKAA 362

HSP 2 Score: 101.7 bits (252), Expect = 1.4e-20
Identity = 48/94 (51.06%), Postives = 60/94 (63.83%), Query Frame = 1


Query: 1  ALKDAWNDACASDRPSAVVIPKGTFKVREGEFKGPCKSPIEFRVHGTLQAPKHP---HGD 60
          AL  AW +ACAS  PS +++PKG F V     +GPCKS I  ++ GTL+AP  P    G 
Sbjct: 5  ALTTAWKEACASASPSTILVPKGNFAVGLITLEGPCKSSIGLQLQGTLKAPADPSKIKGL 64

Query: 61 SWITFAYVDRLRLSGGGVFDGQGKAGWEKNDCHK 92
           WI    +D L + GGGVFDGQGK+ W +NDCHK
Sbjct: 65 GWINLNKIDLLTIFGGGVFDGQGKSAWVQNDCHK 98

[Close]

BLAST of Cucsa.181130 vs. Swiss-Prot
Match: PGLR_GOSHI (Polygalacturonase OS=Gossypium hirsutum GN=G9 PE=2 SV=1)

HSP 1 Score: 238.0 bits (606), Expect = 1.3e-61
Identity = 107/182 (58.79%), Postives = 141/182 (77.47%), Query Frame = 1


Query: 101 VTTGDDCISLGDGSKRIKVNNVICGPGHGISIGSLGKYNNEEPVEGVIVNNCTIINTTNG 160
           + TGDDCIS+GDG+K + +  + CGPGHGISIGSLGK+ NEEPVEG+ ++NCTI NT+NG
Sbjct: 217 IKTGDDCISIGDGTKNMVIKEITCGPGHGISIGSLGKFQNEEPVEGIKISNCTITNTSNG 276

Query: 161 VRIKTWPASPVAGIATNMHFSDITMVNVSNPILIDQEYCPWNQCNREIPSNIKINKVSFK 220
            RIKTWP     G  + +HF DITM NVS+PILIDQ+YCPWN+C +   S +K++ +SFK
Sbjct: 277 ARIKTWPGEH-GGAVSEIHFEDITMNNVSSPILIDQQYCPWNKCKKNEESKVKLSNISFK 336

Query: 221 NIRGSSATPVAVKLICSSNLPCEEVKVANIDLVYNGIKGSITSECMNVKPIISGIQNPPI 280
           NIRG+SA P A+K ICS + PC+ V++A+ID+ +NG + + TS+C+NVKPI SG  NP  
Sbjct: 337 NIRGTSALPEAIKFICSGSSPCQNVELADIDIKHNGAEPA-TSQCLNVKPITSGKLNPIP 396

Query: 281 CS 283
           CS
Sbjct: 397 CS 396

HSP 2 Score: 107.5 bits (267), Expect = 2.6e-22
Identity = 49/100 (49.00%), Postives = 65/100 (65.00%), Query Frame = 1


Query: 4   DAWNDACASDRPSAVVIPKGTFKVREGEFKGPCKSPIEFRVHGTLQAPKHPHG---DSWI 63
           DAW +ACAS  PS VVIPKGT+ + +   +GPCK+PIE  V GT+QAP  P      +W+
Sbjct: 51  DAWKEACASVTPSTVVIPKGTYLLSKVNLEGPCKAPIEINVQGTIQAPADPSAFKDPNWV 110

Query: 64  TFAYVDRLRLSGGGVFDGQGKAGWEKNDCHKRIDCAKLPI 101
            F  V+  ++ GGG+FDGQG   +EKN C  R   +KLP+
Sbjct: 111 RFYSVENFKMFGGGIFDGQGSIAYEKNTCENREFRSKLPV 150

[Close]

BLAST of Cucsa.181130 vs. Swiss-Prot
Match: PGLR_GOSBA (Polygalacturonase OS=Gossypium barbadense GN=G9 PE=2 SV=1)

HSP 1 Score: 235.7 bits (600), Expect = 6.3e-61
Identity = 106/182 (58.24%), Postives = 140/182 (76.92%), Query Frame = 1


Query: 101 VTTGDDCISLGDGSKRIKVNNVICGPGHGISIGSLGKYNNEEPVEGVIVNNCTIINTTNG 160
           + TGDDCIS+GDG+K + +  + CGPGHGISIGSLGK+ NEEPVEG+ ++NCTI NT+NG
Sbjct: 217 IKTGDDCISIGDGTKNMVIKEITCGPGHGISIGSLGKFQNEEPVEGIKISNCTITNTSNG 276

Query: 161 VRIKTWPASPVAGIATNMHFSDITMVNVSNPILIDQEYCPWNQCNREIPSNIKINKVSFK 220
            RIKTWP     G  + +HF DITM NVS+PILIDQ+YCPWN+C +   S +K++ +SFK
Sbjct: 277 ARIKTWPGEH-GGAVSEIHFEDITMNNVSSPILIDQQYCPWNKCKKNEESKVKLSNISFK 336

Query: 221 NIRGSSATPVAVKLICSSNLPCEEVKVANIDLVYNGIKGSITSECMNVKPIISGIQNPPI 280
           NIRG+SA P A+K ICS + PC+ V++A+ID+ +NG + + TS+C+NVKPI  G  NP  
Sbjct: 337 NIRGTSALPEAIKFICSGSSPCQNVELADIDIQHNGAEPA-TSQCLNVKPITIGKLNPIP 396

Query: 281 CS 283
           CS
Sbjct: 397 CS 396

HSP 2 Score: 107.5 bits (267), Expect = 2.6e-22
Identity = 49/100 (49.00%), Postives = 65/100 (65.00%), Query Frame = 1


Query: 4   DAWNDACASDRPSAVVIPKGTFKVREGEFKGPCKSPIEFRVHGTLQAPKHPHG---DSWI 63
           DAW +ACAS  PS VVIPKGT+ + +   +GPCK+PIE  V GT+QAP  P      +W+
Sbjct: 51  DAWKEACASVTPSTVVIPKGTYLLSKVNLEGPCKAPIEINVQGTIQAPADPSAFKDPNWV 110

Query: 64  TFAYVDRLRLSGGGVFDGQGKAGWEKNDCHKRIDCAKLPI 101
            F  V+  ++ GGG+FDGQG   +EKN C  R   +KLP+
Sbjct: 111 RFYSVENFKMFGGGIFDGQGSIAYEKNTCENREFRSKLPV 150

[Close]

BLAST of Cucsa.181130 vs. Swiss-Prot
Match: PGLR_TOBAC (Polygalacturonase OS=Nicotiana tabacum GN=PG1 PE=2 SV=1)

HSP 1 Score: 228.8 bits (582), Expect = 7.7e-59
Identity = 102/188 (54.26%), Postives = 143/188 (76.06%), Query Frame = 1


Query: 102 TTGDDCISLGDGSKRIKVNNVICGPGHGISIGSLGKYNNEEPVEGVIVNNCTIINTTNGV 161
           +TGDDCIS+GD ++++ +  V CGPGHGIS+GSLG   +E+PV GV V NCT  NT NGV
Sbjct: 210 STGDDCISVGDETEQLYITRVTCGPGHGISVGSLGGNPDEKPVVGVFVRNCTFTNTDNGV 269

Query: 162 RIKTWPASPVAGIATNMHFSDITMVNVSNPILIDQEYCPWNQCNREIPSNIKINKVSFKN 221
           RIKTWPAS   G+  ++HF DI + NVSNP++IDQ YCP+N+CN+++PS +KI+KVSF+N
Sbjct: 270 RIKTWPASH-PGVVNDVHFEDIIVQNVSNPVVIDQVYCPFNKCNKDLPSQVKISKVSFQN 329

Query: 222 IRGSSATPVAVKLICSSNLPCEEVKVANIDLVYNGIKGSITSECMNVKPIISGIQNPPIC 281
           I+G+S T  AV L+ S  +PCE ++V +ID+ Y+G +G   S C N+KP + G QNPP+C
Sbjct: 330 IKGTSRTQDAVSLLRSKGVPCEGIEVGDIDITYSGKEGPAKSSCENIKPSLKGKQNPPVC 389

Query: 282 SSSYTTAS 290
           ++S  ++S
Sbjct: 390 TASAASSS 396

HSP 2 Score: 94.7 bits (234), Expect = 1.7e-18
Identity = 45/102 (44.12%), Postives = 63/102 (61.76%), Query Frame = 1


Query: 1   ALKDAWNDACASDRPSAVVIPKGTFKVREGEFKGPCKSPIEFRVHGTLQAPKHPHG---D 60
           AL +A+ +AC S  PS +VIPKGTF + + + +GPCKSP+E ++  TL+AP  P      
Sbjct: 43  ALLNAFKEACQSTSPSTIVIPKGTFTMNQVKLEGPCKSPLELQIQATLKAPSDPSQLKVG 102

Query: 61  SWITFAYVDRLRLSGGGVFDGQGKAGWEKNDCHKRIDCAKLP 100
            W+T   +D+  +SGGG+ DGQ  A WE   C +   C KLP
Sbjct: 103 EWLTVNKLDQFTMSGGGILDGQAAAAWE---CKQSKKCNKLP 141

[Close]

BLAST of Cucsa.181130 vs. Swiss-Prot
Match: PGLR2_ARATH (Exopolygalacturonase clone GBGA483 OS=Arabidopsis thaliana GN=At3g07850 PE=2 SV=2)

HSP 1 Score: 227.6 bits (579), Expect = 1.7e-58
Identity = 105/182 (57.69%), Postives = 137/182 (75.27%), Query Frame = 1


Query: 101 VTTGDDCISLGDGSKRIKVNNVICGPGHGISIGSLGKYNNEEPVEGVIVNNCTIINTTNG 160
           + TGDDC+S+GDG++ + V NV CGPGHGISIGSLG+Y NE+PV+GV V  C I NT NG
Sbjct: 257 IKTGDDCVSIGDGTENLIVENVECGPGHGISIGSLGRYPNEQPVKGVTVRKCLIKNTDNG 316

Query: 161 VRIKTWPASPVAGIATNMHFSDITMVNVSNPILIDQEYCPWNQCNREIPSNIKINKVSFK 220
           VRIKTWP SP  GIA+N+ F DITM NVS P+LIDQEYCP+  C   +PS +K++ V+ K
Sbjct: 317 VRIKTWPGSP-PGIASNILFEDITMDNVSLPVLIDQEYCPYGHCKAGVPSQVKLSDVTIK 376

Query: 221 NIRGSSATPVAVKLICSSNLPCEEVKVANIDLVYNGIKGSITSECMNVKPIISGIQNPPI 280
            I+G+SAT VAVKL+CS  +PC  + +++I+LV+NG +G   S C N+KPI+SG   P  
Sbjct: 377 GIKGTSATKVAVKLMCSKGVPCTNIALSDINLVHNGKEGPAVSACSNIKPILSGKLVPAA 436

Query: 281 CS 283
           C+
Sbjct: 437 CT 437

HSP 2 Score: 83.2 bits (204), Expect = 5.2e-15
Identity = 43/106 (40.57%), Postives = 56/106 (52.83%), Query Frame = 1


Query: 1   ALKDAWNDACASDRPSAVVIPKGTFKVREGEFKGPCKSPIEFRVHGTLQAP-----KHPH 60
           A   AW +ACA+   S + +PKG + V   EFKGPCK P+   ++G  +AP       PH
Sbjct: 88  AFAAAWKEACAAG--STITVPKGEYMVESLEFKGPCKGPVTLELNGNFKAPATVKTTKPH 147

Query: 61  GDSWITFAYVDRLRLSGG-GVFDGQGKAGWEKNDCHKRIDCAKLPI 101
              WI F  +    L+G   +FDGQG   W+ NDC K   C  LPI
Sbjct: 148 A-GWIDFENIADFTLNGNKAIFDGQGSLAWKANDCAKTGKCNSLPI 190

[Close]

BLAST of Cucsa.181130 vs. TrEMBL
Match: A0A0A0K3B7_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_7G037910 PE=3 SV=1)

HSP 1 Score: 389.4 bits (999), Expect = 3.8e-105
Identity = 188/190 (98.95%), Postives = 189/190 (99.47%), Query Frame = 1


Query: 101 VTTGDDCISLGDGSKRIKVNNVICGPGHGISIGSLGKYNNEEPVEGVIVNNCTIINTTNG 160
           + TGDDCISLGDGSKRIKVNNVICGPGHGISIGSLGKYNNEEPVEGVIVNNCTIINTTNG
Sbjct: 215 IATGDDCISLGDGSKRIKVNNVICGPGHGISIGSLGKYNNEEPVEGVIVNNCTIINTTNG 274

Query: 161 VRIKTWPASPVAGIATNMHFSDITMVNVSNPILIDQEYCPWNQCNREIPSNIKINKVSFK 220
           VRIKTWPASPVAGIATNMHFSDITMVNVSNPILIDQEYCPWNQCNREIPSNIKINKVSFK
Sbjct: 275 VRIKTWPASPVAGIATNMHFSDITMVNVSNPILIDQEYCPWNQCNREIPSNIKINKVSFK 334

Query: 221 NIRGSSATPVAVKLICSSNLPCEEVKVANIDLVYNGIKGSITSECMNVKPIISGIQNPPI 280
           NIRGSSATPVAVKLICSSNLPCEEVKVANIDLVYNGIKGSITSECMNVKPIISGIQNPPI
Sbjct: 335 NIRGSSATPVAVKLICSSNLPCEEVKVANIDLVYNGIKGSITSECMNVKPIISGIQNPPI 394

Query: 281 CSSSYTTASK 291
           CSSSYTTASK
Sbjct: 395 CSSSYTTASK 404

[Close]

BLAST of Cucsa.181130 vs. TrEMBL
Match: A0A0A0K3B7_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_7G037910 PE=3 SV=1)

HSP 1 Score: 218.8 bits (556), Expect = 8.8e-54
Identity = 99/100 (99.00%), Postives = 100/100 (100.00%), Query Frame = 1


Query: 1   ALKDAWNDACASDRPSAVVIPKGTFKVREGEFKGPCKSPIEFRVHGTLQAPKHPHGDSWI 60
           ALKDAWNDACASDRPSAVVIPKGTFKVREGEFKGPCKSPIEFRVHGTLQAPKHPHGDSWI
Sbjct: 49  ALKDAWNDACASDRPSAVVIPKGTFKVREGEFKGPCKSPIEFRVHGTLQAPKHPHGDSWI 108

Query: 61  TFAYVDRLRLSGGGVFDGQGKAGWEKNDCHKRIDCAKLPI 101
           TFAYVDR+RLSGGGVFDGQGKAGWEKNDCHKRIDCAKLPI
Sbjct: 109 TFAYVDRMRLSGGGVFDGQGKAGWEKNDCHKRIDCAKLPI 148

HSP 2 Score: 322.4 bits (825), Expect = 5.7e-85
Identity = 161/292 (55.14%), Postives = 200/292 (68.49%), Query Frame = 1


Query: 1   ALKDAWNDACASDRPSAVVIPKGTFKVREGEFKGPCKSPIEFRVHGTLQAP----KHPHG 60
           AL  AW DAC+S   S V+IP GT+ + +    GPCK+PI F V GT+ AP    +    
Sbjct: 46  ALLKAWGDACSSPVASTVMIPDGTYALGQIIIAGPCKAPINFVVQGTVMAPVDTSRFRAE 105

Query: 61  DSWITFAYVDRLRLSGGGVFDGQGKAGWEKNDCHKRIDCAKLPIVT------TGDDCISL 120
             WITF  +D+  LSG GVFDGQGK  W    C     C +LPI T      TGDDC+S+
Sbjct: 106 AGWITFQQIDQFTLSGDGVFDGQGKIVWGTK-CLSSAYCNQLPINTNTISFETGDDCVSI 165

Query: 121 GDGSKRIKVNNVICGPGHGISIGSLGKYNNEEPVEGVIVNNCTIINTTNGVRIKTWPASP 180
           GDGS++I +  V CGPGHGIS+GSLGKY NEEPV G+ V NCT+ NT NGVR+KTWPAS 
Sbjct: 166 GDGSEQINIQRVTCGPGHGISVGSLGKYPNEEPVVGISVKNCTLTNTQNGVRVKTWPASH 225

Query: 181 VAGIATNMHFSDITMVNVSNPILIDQEYCPWNQCNREIPSNIKINKVSFKNIRGSSATPV 240
             G A+ MHF DI M NV NPI+IDQEYCP NQCN + PS IKI+ VSF+NIRG+++T V
Sbjct: 226 -QGTASEMHFEDIVMNNVGNPIIIDQEYCPHNQCNLKSPSRIKISNVSFRNIRGTTSTQV 285

Query: 241 AVKLICSSNLPCEEVKVANIDLVYNGIKGSITSECMNVKPIISGIQNPPICS 283
           AVKLICS  +PC++V++ +I+L YNG  G   S+C N+KP + G Q P IC+
Sbjct: 286 AVKLICSQGVPCQDVELGDINLEYNGNDGPAMSQCKNIKPNVLGAQLPRICA 335

[Close]

BLAST of Cucsa.181130 vs. TrEMBL
Match: A0A0B2SJ99_GLYSO (Polygalacturonase (Fragment) OS=Glycine soja GN=glysoja_050108 PE=3 SV=1)

HSP 1 Score: 321.2 bits (822), Expect = 1.3e-84
Identity = 158/301 (52.49%), Postives = 202/301 (67.11%), Query Frame = 1


Query: 5   AWNDACASDRPSAVVIPKGTFKVREGEFKGPCKSPIEFRVHGTLQAPKHPH----GDSWI 64
           AW  ACAS     +VIP GT+++   + KGPCK+PIE +V GT+QAP +P        W 
Sbjct: 2   AWTQACASPTAVKIVIPAGTYQMGAVDVKGPCKAPIEVQVDGTIQAPANPTDLKAAHQWF 61

Query: 65  TFAYVDRLRLSGGGVFDGQGKAGWEKNDCHKRIDCAKLPIVT------------------ 124
              YV+   LSG GVFDGQG   W++NDC    +C  L + T                  
Sbjct: 62  VVQYVNSFTLSGKGVFDGQGATAWKQNDCTTNKNCKMLCMPTKPHIGRSTDVKILNTNIA 121

Query: 125 TGDDCISLGDGSKRIKVNNVICGPGHGISIGSLGKYNNEEPVEGVIVNNCTIINTTNGVR 184
           TGDDC+SLGDG K I V NV CGPGHGIS+GSLGKY++EEPV G +V NCT+  T NGVR
Sbjct: 122 TGDDCVSLGDGCKNITVQNVNCGPGHGISVGSLGKYDSEEPVAGFLVKNCTLNGTDNGVR 181

Query: 185 IKTWPASPVAGIATNMHFSDITMVNVSNPILIDQEYCPWNQCNREIPSNIKINKVSFKNI 244
           IKTWP +P A   T+MHF D+TM NV+NPI+IDQEYCPWNQC+++ PS IKI+KVSFKNI
Sbjct: 182 IKTWPNTPGAITITDMHFEDLTMNNVTNPIIIDQEYCPWNQCSKQNPSKIKISKVSFKNI 241

Query: 245 RGSSATPVAVKLICSSNLPCEEVKVANIDLVYNGIKGSITSECMNVKPIISGIQNPPICS 284
           +G+S +   V L+CSS +PCE V++A+IDL +NG   + T++C NVKP I+G    P C+
Sbjct: 242 KGTSGSQEGVVLVCSSGVPCEGVEMADIDLTFNG--AAATAKCANVKPTITG--KAPTCA 298

[Close]

BLAST of Cucsa.181130 vs. TrEMBL
Match: A0A078G9V9_BRANA (BnaC01g39620D protein OS=Brassica napus GN=BnaC01g39620D PE=3 SV=1)

HSP 1 Score: 298.1 bits (762), Expect = 1.1e-77
Identity = 145/323 (44.89%), Postives = 190/323 (58.82%), Query Frame = 1


Query: 1   ALKDAWNDACASDRPSAVVIPKGTFKVREGEFKGPCKSPIEFRVHGTLQAP--KHPHGDS 60
           A+  A+  AC +  PS V+IPKG FK+ E    GPCKSPIEF + G ++A        D 
Sbjct: 26  AVLKAFTSACQAPAPSQVLIPKGDFKLGETVMTGPCKSPIEFTLQGNVKADGGSTQGKDR 85

Query: 61  WITFAYVDRLRLSGGGVFDGQGKAGWEKNDCHKRIDCAKLPI------------------ 120
           W+ F  ++  +L+GGG FDG+G A W+ N+CHK  +C KLPI                  
Sbjct: 86  WVVFEKINGFKLNGGGTFDGEGNAAWKANNCHKTFECKKLPISVSGKNMTFDNIKIIAPA 145

Query: 121 ----------------------VTTGDDCISLGDGSKRIKVNNVICGPGHGISIGSLGKY 180
                                 + TGDDCIS+GDG K + +  V+CGPGHGIS+GSLG+Y
Sbjct: 146 ESPNTDGIHLGRCEGVKILNTKIATGDDCISVGDGMKNLLIEKVVCGPGHGISVGSLGRY 205

Query: 181 NNEEPVEGVIVNNCTIINTTNGVRIKTWPASPVAGIATNMHFSDITMVNVSNPILIDQEY 240
             E+ V  + V NCT+  T NG+RIKTWP++     A  +HF DI +  VSNPILIDQEY
Sbjct: 206 GWEQDVTDINVKNCTLEGTDNGLRIKTWPSAACTTTAAGIHFEDIILNKVSNPILIDQEY 265

Query: 241 CPWNQCNREIPSNIKINKVSFKNIRGSSATPVAVKLICSSNLPCEEVKVANIDLVYNGIK 282
           CPWNQCN+  PS IK+  ++F+NIRG+S    AVKL+CS   PCE V++ +I++ Y G  
Sbjct: 266 CPWNQCNKNKPSTIKLVDITFRNIRGTSGNKDAVKLLCSKGHPCENVEIGDINIEYTGPD 325

[Close]

BLAST of Cucsa.181130 vs. TrEMBL
Match: A0A0D3BA84_BRAOL (Uncharacterized protein OS=Brassica oleracea var. oleracea PE=3 SV=1)

HSP 1 Score: 297.0 bits (759), Expect = 2.6e-77
Identity = 144/323 (44.58%), Postives = 191/323 (59.13%), Query Frame = 1


Query: 1   ALKDAWNDACASDRPSAVVIPKGTFKVREGEFKGPCKSPIEFRVHGTLQAP--KHPHGDS 60
           AL  A+  AC +  PS V+IPKG FK+ E    GPCKSP+EF + G ++A        D 
Sbjct: 20  ALLKAFTSACQAPAPSQVLIPKGDFKLGEIAMTGPCKSPVEFTLQGNVKADGGSTQGKDR 79

Query: 61  WITFAYVDRLRLSGGGVFDGQGKAGWEKNDCHKRIDCAKLPI------------------ 120
           W+ F  ++  +L+GGG FDG+G A W+ N+CHK  +C KLPI                  
Sbjct: 80  WVVFEKINGFKLNGGGTFDGEGNAAWKANNCHKTFECKKLPISVSGKNMTFDNIKIIAPA 139

Query: 121 ----------------------VTTGDDCISLGDGSKRIKVNNVICGPGHGISIGSLGKY 180
                                 ++TGDDCIS+GDG K + +  V+CGPGHGIS+GSLG+Y
Sbjct: 140 ESPNTDGIHLGRCEGVKILNTKISTGDDCISVGDGMKNLLIEKVVCGPGHGISVGSLGRY 199

Query: 181 NNEEPVEGVIVNNCTIINTTNGVRIKTWPASPVAGIATNMHFSDITMVNVSNPILIDQEY 240
             E+ V  + V NCT+  T NG+RIKTWP++     A  +HF +I +  VSNPILIDQEY
Sbjct: 200 GWEQDVNDIKVINCTLEGTDNGLRIKTWPSAACTTTAAGIHFENIILNKVSNPILIDQEY 259

Query: 241 CPWNQCNREIPSNIKINKVSFKNIRGSSATPVAVKLICSSNLPCEEVKVANIDLVYNGIK 282
           CPWNQCN+  PS IK+  ++F+NIRG+S    AVKL+CS   PCE V++ +I++ Y G  
Sbjct: 260 CPWNQCNKSKPSTIKLVDITFRNIRGTSGNKDAVKLLCSKGHPCENVEIGDINIEYTGAD 319

[Close]

BLAST of Cucsa.181130 vs. TAIR10
Match: AT3G07840.1 (AT3G07840.1 Pectin lyase-like superfamily protein)

HSP 1 Score: 231.5 bits (589), Expect = 6.7e-61
Identity = 109/192 (56.77%), Postives = 138/192 (71.88%), Query Frame = 1


Query: 101 VTTGDDCISLGDGSKRIKVNNVICGPGHGISIGSLGKYNNEEPVEGVIVNNCTIINTTNG 160
           + TGDDCIS+GDG K + V NV+CGPGHGIS+GSLG+Y +E+ V G+ V NCT+  T NG
Sbjct: 207 IATGDDCISVGDGMKNLHVENVMCGPGHGISVGSLGRYVHEQDVTGITVVNCTLQGTDNG 266

Query: 161 VRIKTWPASPVAGIATNMHFSDITMVNVSNPILIDQEYCPWNQCNREIPSNIKINKVSFK 220
           +RIKTWP++  A  A+ +HF +I + NVSNPILIDQEYCPWNQCN++ PS IK+  +SFK
Sbjct: 267 LRIKTWPSAACATTASGIHFENIILNNVSNPILIDQEYCPWNQCNKQKPSTIKLVDISFK 326

Query: 221 NIRGSSATPVAVKLICSSNLPCEEVKVANIDLVYNGIKGSITSECMNVKPIISGIQNPPI 280
           NIRG+S    AVKL+CS   PC  V++ NI+L Y G  G  T  C NV P + G QNP  
Sbjct: 327 NIRGTSGNKDAVKLLCSKAHPCANVEIGNINLEYKGADGPPTFMCSNVSPKLVGTQNPKA 386

Query: 281 CSSSYT--TASK 291
           CS+  T  TA+K
Sbjct: 387 CSAGPTPKTAAK 398

HSP 2 Score: 114.8 bits (286), Expect = 9.1e-26
Identity = 104/360 (28.89%), Postives = 155/360 (43.06%), Query Frame = 1


Query: 1   ALKDAWNDACASDRPSAVVIPKGTFKVREGEFKGPCKSPIEFRVHGTLQAP-KHPHG-DS 60
           AL  A+  AC +   S VVI KG FK+ E E  GPCK+P+E  + GTL+A  K   G + 
Sbjct: 39  ALLKAFTSACQAPTASKVVITKGEFKLGEIEMTGPCKAPVEINLQGTLKADGKAIQGKER 98

Query: 61  WITFAYVDRLRLSGGGVFDGQGKAGWEKNDCHKRIDCAKLPIVTTGD--------DCISL 120
           W+ F  ++  +L+GGG+FDG+G A W  N+CHK  +C KLPI    D        D  S+
Sbjct: 99  WVVFLRINGFKLNGGGIFDGEGNAAWRVNNCHKTFECKKLPISIRFDFVENAEIRDISSI 158

Query: 121 GD--------GSKRIKVNNV-ICGPGHGISIGS--LGKYNNEEPVEGVIVNNCTIINTTN 180
                     G+K +  +NV +  P    +     LG+    + +   I      I+  +
Sbjct: 159 DAKNFHINVLGAKNMTFDNVKVIAPAESPNTDGIHLGRSEGVKILNSKIATGDDCISVGD 218

Query: 181 GVR---IKTWPASPVAGIATN-----MHFSDITMVNVSNPIL------IDQEYCPWNQCN 240
           G++   ++     P  GI+       +H  D+T + V N  L      +  +  P   C 
Sbjct: 219 GMKNLHVENVMCGPGHGISVGSLGRYVHEQDVTGITVVNCTLQGTDNGLRIKTWPSAACA 278

Query: 241 REIP----SNIKINKVSFKNI---------RGSSATPVAVKLICSS-------------- 291
                    NI +N VS   +         + +   P  +KL+  S              
Sbjct: 279 TTASGIHFENIILNNVSNPILIDQEYCPWNQCNKQKPSTIKLVDISFKNIRGTSGNKDAV 338

[Close]

BLAST of Cucsa.181130 vs. TAIR10
Match: AT3G14040.1 (AT3G14040.1 Pectin lyase-like superfamily protein)

HSP 1 Score: 229.9 bits (585), Expect = 1.9e-60
Identity = 106/182 (58.24%), Postives = 138/182 (75.82%), Query Frame = 1


Query: 101 VTTGDDCISLGDGSKRIKVNNVICGPGHGISIGSLGKYNNEEPVEGVIVNNCTIINTTNG 160
           + TGDDC+S+GDG++ + V NV CGPGHGISIGSLG+Y NE+PV+GV V  C I NT NG
Sbjct: 258 IKTGDDCVSIGDGTENLIVENVECGPGHGISIGSLGRYPNEQPVKGVTVRKCLIKNTDNG 317

Query: 161 VRIKTWPASPVAGIATNMHFSDITMVNVSNPILIDQEYCPWNQCNREIPSNIKINKVSFK 220
           VRIKTWP SP  GIA+N+ F DITM NVS P+LIDQEYCP+  C   +PS +K++ V+ K
Sbjct: 318 VRIKTWPGSP-PGIASNILFEDITMDNVSLPVLIDQEYCPYGHCKAGVPSKVKLSDVTIK 377

Query: 221 NIRGSSATPVAVKLICSSNLPCEEVKVANIDLVYNGIKGSITSECMNVKPIISGIQNPPI 280
           NI+G+SAT VAVKL+CS  +PC  + +++I+LV+NG +G   S C N+KPI+SG   P  
Sbjct: 378 NIKGTSATKVAVKLMCSKGVPCTNIALSDINLVHNGKEGPAVSACSNIKPILSGKLVPAA 437

Query: 281 CS 283
           C+
Sbjct: 438 CT 438

HSP 2 Score: 82.0 bits (201), Expect = 6.5e-16
Identity = 43/106 (40.57%), Postives = 56/106 (52.83%), Query Frame = 1


Query: 1   ALKDAWNDACASDRPSAVVIPKGTFKVREGEFKGPCKSPIEFRVHGTLQAP-----KHPH 60
           A   AW +ACA+   S + +PKG + V   EFKGPCK P+   ++G  +AP       PH
Sbjct: 89  AFAAAWKEACAAG--STITVPKGEYLVESLEFKGPCKGPVTLELNGNFKAPATVKTTKPH 148

Query: 61  GDSWITFAYVDRLRLSGG-GVFDGQGKAGWEKNDCHKRIDCAKLPI 101
              WI F  +    L+G   +FDGQG   W+ NDC K   C  LPI
Sbjct: 149 A-GWIDFENLADFTLNGNKAIFDGQGSLAWKANDCAKTGKCNSLPI 191

[Close]

BLAST of Cucsa.181130 vs. TAIR10
Match: AT3G07820.1 (AT3G07820.1 Pectin lyase-like superfamily protein)

HSP 1 Score: 229.2 bits (583), Expect = 3.3e-60
Identity = 102/183 (55.74%), Postives = 134/183 (73.22%), Query Frame = 1


Query: 101 VTTGDDCISLGDGSKRIKVNNVICGPGHGISIGSLGKYNNEEPVEGVIVNNCTIINTTNG 160
           ++TGDDCIS+GDG K + V  V CGPGHGIS+GSLG+Y +E+ V G+ V NCT+  T NG
Sbjct: 206 ISTGDDCISVGDGMKNLHVEKVTCGPGHGISVGSLGRYGHEQDVSGIKVINCTLQETDNG 265

Query: 161 VRIKTWPASPVAGIATNMHFSDITMVNVSNPILIDQEYCPWNQCNREIPSNIKINKVSFK 220
           +RIKTWP++  +  A+++HF DI + +VSNPILIDQEYCPWNQCN++  S IK+  +SFK
Sbjct: 266 LRIKTWPSAACSTTASDIHFEDIILKDVSNPILIDQEYCPWNQCNKQKASTIKLVNISFK 325

Query: 221 NIRGSSATPVAVKLICSSNLPCEEVKVANIDLVYNGIKGSITSECMNVKPIISGIQNPPI 280
           NIRG+S    AVKL+CS   PC+ V++ +ID+ YNG  G  T  C NV P I G Q+P  
Sbjct: 326 NIRGTSGNKDAVKLLCSKGYPCQNVEIGDIDIKYNGADGPATFHCSNVSPKILGSQSPKA 385

Query: 281 CSS 284
           CS+
Sbjct: 386 CSA 388

HSP 2 Score: 110.2 bits (274), Expect = 2.2e-24
Identity = 98/357 (27.45%), Postives = 149/357 (41.74%), Query Frame = 1


Query: 1   ALKDAWNDACASDRPSAVVIPKGTFKVREGEFKGPCKSPIEFRVHGTLQAPKHP--HGDS 60
           AL  A+  AC S  PS VVIPKG FK+ E E +GPCK+PIE  + GT++A  +     + 
Sbjct: 38  ALLKAFTSACQSSSPSKVVIPKGEFKLGEIEMRGPCKAPIEVTLQGTVKADGNAIQGKEK 97

Query: 61  WITFAYVDRLRLSGGGVFDGQGKAGWEKNDCHKRIDCAKLPIVTTGD--------DCISL 120
           W+ F  +D  +L+GGG FDG+G A W  N+CHK  +C KLPI    D        D  S+
Sbjct: 98  WVVFGNIDGFKLNGGGAFDGEGNAAWRVNNCHKTFECKKLPISIRFDFILNSEIRDISSI 157

Query: 121 GD--------GSKRIKVNNV-ICGPGHGISIGS--LGKYNNEEPVEGVIVNNCTIINTTN 180
                     G+K + +NN+ I  P    +     LG+ +  + +   I      I+  +
Sbjct: 158 DAKNFHINVLGAKNMTMNNIKIVAPEDSPNTDGIHLGRSDGVKILNSFISTGDDCISVGD 217

Query: 181 GVR---IKTWPASPVAGIATNM-----HFSDITMVNVSNPILIDQE------YCPWNQCN 240
           G++   ++     P  GI+        H  D++ + V N  L + +        P   C+
Sbjct: 218 GMKNLHVEKVTCGPGHGISVGSLGRYGHEQDVSGIKVINCTLQETDNGLRIKTWPSAACS 277

Query: 241 REIPSNIKINKVSFKNIRGSSATPVAV-KLICSSNLPCEEVKVANIDLVYNGIK------ 284
               S+I    +  K++    + P+ + +  C  N  C + K + I LV    K      
Sbjct: 278 -TTASDIHFEDIILKDV----SNPILIDQEYCPWN-QCNKQKASTIKLVNISFKNIRGTS 337

[Close]

BLAST of Cucsa.181130 vs. TAIR10
Match: AT5G48140.1 (AT5G48140.1 Pectin lyase-like superfamily protein)

HSP 1 Score: 228.0 bits (580), Expect = 7.4e-60
Identity = 102/190 (53.68%), Postives = 135/190 (71.05%), Query Frame = 1


Query: 101 VTTGDDCISLGDGSKRIKVNNVICGPGHGISIGSLGKYNNEEPVEGVIVNNCTIINTTNG 160
           ++TGDDC+S+GDG K + V  V CGPGHGISIGSLG+Y++EE V G+ + NCT+  T NG
Sbjct: 206 ISTGDDCVSVGDGMKNLLVERVTCGPGHGISIGSLGRYSHEENVSGIKIINCTLQETDNG 265

Query: 161 VRIKTWPASPVAGIATNMHFSDITMVNVSNPILIDQEYCPWNQCNREIPSNIKINKVSFK 220
           +RIKTWP++     A+++HF +I + NVSNPILIDQEYCPWNQCN++ PS IK+  +SFK
Sbjct: 266 LRIKTWPSAACTTTASDIHFENILLKNVSNPILIDQEYCPWNQCNKQKPSTIKLANISFK 325

Query: 221 NIRGSSATPVAVKLICSSNLPCEEVKVANIDLVYNGIKGSITSECMNVKPIISGIQNPPI 280
            IRG+S    AVKL+CS   PC+ V+V ++++ Y G  G  T +C NV P + G Q P  
Sbjct: 326 KIRGTSGNKDAVKLLCSKGYPCQNVEVGDVNIQYTGADGPATFQCSNVSPKLVGTQIPKA 385

Query: 281 CSSSYTTASK 291
           CSS  T   K
Sbjct: 386 CSSPVTKPPK 395

HSP 2 Score: 101.3 bits (251), Expect = 1.0e-21
Identity = 46/101 (45.54%), Postives = 66/101 (65.35%), Query Frame = 1


Query: 1   ALKDAWNDACASDRPSAVVIPKGTFKVREGEFKGPCKSPIEFRVHGTLQAPKHPHG-DSW 60
           AL  A+N+AC     S V+IPKG +K+ E    GPCK+PI   + GT++A  + +G + W
Sbjct: 39  ALLKAFNEACQFPTKSTVMIPKGEYKLGEIVMMGPCKAPIRIALLGTVKADGNANGKEKW 98

Query: 61  ITFAYVDRLRLSGGGVFDGQGKAGWEKNDCHKRIDCAKLPI 101
           + F  ++  +L+GGGVFDG+G A W  N+CHK  +C KLPI
Sbjct: 99  VAFRNINGFKLNGGGVFDGEGNAAWRVNNCHKTFNCKKLPI 139

[Close]

BLAST of Cucsa.181130 vs. TAIR10
Match: AT3G07850.1 (AT3G07850.1 Pectin lyase-like superfamily protein)

HSP 1 Score: 227.6 bits (579), Expect = 9.6e-60
Identity = 105/182 (57.69%), Postives = 137/182 (75.27%), Query Frame = 1


Query: 101 VTTGDDCISLGDGSKRIKVNNVICGPGHGISIGSLGKYNNEEPVEGVIVNNCTIINTTNG 160
           + TGDDC+S+GDG++ + V NV CGPGHGISIGSLG+Y NE+PV+GV V  C I NT NG
Sbjct: 257 IKTGDDCVSIGDGTENLIVENVECGPGHGISIGSLGRYPNEQPVKGVTVRKCLIKNTDNG 316

Query: 161 VRIKTWPASPVAGIATNMHFSDITMVNVSNPILIDQEYCPWNQCNREIPSNIKINKVSFK 220
           VRIKTWP SP  GIA+N+ F DITM NVS P+LIDQEYCP+  C   +PS +K++ V+ K
Sbjct: 317 VRIKTWPGSP-PGIASNILFEDITMDNVSLPVLIDQEYCPYGHCKAGVPSQVKLSDVTIK 376

Query: 221 NIRGSSATPVAVKLICSSNLPCEEVKVANIDLVYNGIKGSITSECMNVKPIISGIQNPPI 280
            I+G+SAT VAVKL+CS  +PC  + +++I+LV+NG +G   S C N+KPI+SG   P  
Sbjct: 377 GIKGTSATKVAVKLMCSKGVPCTNIALSDINLVHNGKEGPAVSACSNIKPILSGKLVPAA 436

Query: 281 CS 283
           C+
Sbjct: 437 CT 437

HSP 2 Score: 83.2 bits (204), Expect = 2.9e-16
Identity = 43/106 (40.57%), Postives = 56/106 (52.83%), Query Frame = 1


Query: 1   ALKDAWNDACASDRPSAVVIPKGTFKVREGEFKGPCKSPIEFRVHGTLQAP-----KHPH 60
           A   AW +ACA+   S + +PKG + V   EFKGPCK P+   ++G  +AP       PH
Sbjct: 88  AFAAAWKEACAAG--STITVPKGEYMVESLEFKGPCKGPVTLELNGNFKAPATVKTTKPH 147

Query: 61  GDSWITFAYVDRLRLSGG-GVFDGQGKAGWEKNDCHKRIDCAKLPI 101
              WI F  +    L+G   +FDGQG   W+ NDC K   C  LPI
Sbjct: 148 A-GWIDFENIADFTLNGNKAIFDGQGSLAWKANDCAKTGKCNSLPI 190

[Close]

BLAST of Cucsa.181130 vs. NCBI nr
Match: gi|778730103|ref|XP_011659699.1| (PREDICTED: exopolygalacturonase-like [Cucumis sativus])

HSP 1 Score: 389.4 bits (999), Expect = 5.4e-105
Identity = 188/190 (98.95%), Postives = 189/190 (99.47%), Query Frame = 1


Query: 101 VTTGDDCISLGDGSKRIKVNNVICGPGHGISIGSLGKYNNEEPVEGVIVNNCTIINTTNG 160
           + TGDDCISLGDGSKRIKVNNVICGPGHGISIGSLGKYNNEEPVEGVIVNNCTIINTTNG
Sbjct: 200 IATGDDCISLGDGSKRIKVNNVICGPGHGISIGSLGKYNNEEPVEGVIVNNCTIINTTNG 259

Query: 161 VRIKTWPASPVAGIATNMHFSDITMVNVSNPILIDQEYCPWNQCNREIPSNIKINKVSFK 220
           VRIKTWPASPVAGIATNMHFSDITMVNVSNPILIDQEYCPWNQCNREIPSNIKINKVSFK
Sbjct: 260 VRIKTWPASPVAGIATNMHFSDITMVNVSNPILIDQEYCPWNQCNREIPSNIKINKVSFK 319

Query: 221 NIRGSSATPVAVKLICSSNLPCEEVKVANIDLVYNGIKGSITSECMNVKPIISGIQNPPI 280
           NIRGSSATPVAVKLICSSNLPCEEVKVANIDLVYNGIKGSITSECMNVKPIISGIQNPPI
Sbjct: 320 NIRGSSATPVAVKLICSSNLPCEEVKVANIDLVYNGIKGSITSECMNVKPIISGIQNPPI 379

Query: 281 CSSSYTTASK 291
           CSSSYTTASK
Sbjct: 380 CSSSYTTASK 389

[Close]

BLAST of Cucsa.181130 vs. NCBI nr
Match: gi|778730103|ref|XP_011659699.1| (PREDICTED: exopolygalacturonase-like [Cucumis sativus])

HSP 1 Score: 218.8 bits (556), Expect = 1.3e-53
Identity = 99/100 (99.00%), Postives = 100/100 (100.00%), Query Frame = 1


Query: 1   ALKDAWNDACASDRPSAVVIPKGTFKVREGEFKGPCKSPIEFRVHGTLQAPKHPHGDSWI 60
           ALKDAWNDACASDRPSAVVIPKGTFKVREGEFKGPCKSPIEFRVHGTLQAPKHPHGDSWI
Sbjct: 34  ALKDAWNDACASDRPSAVVIPKGTFKVREGEFKGPCKSPIEFRVHGTLQAPKHPHGDSWI 93

Query: 61  TFAYVDRLRLSGGGVFDGQGKAGWEKNDCHKRIDCAKLPI 101
           TFAYVDR+RLSGGGVFDGQGKAGWEKNDCHKRIDCAKLPI
Sbjct: 94  TFAYVDRMRLSGGGVFDGQGKAGWEKNDCHKRIDCAKLPI 133

HSP 2 Score: 389.4 bits (999), Expect = 5.4e-105
Identity = 188/190 (98.95%), Postives = 189/190 (99.47%), Query Frame = 1


Query: 101 VTTGDDCISLGDGSKRIKVNNVICGPGHGISIGSLGKYNNEEPVEGVIVNNCTIINTTNG 160
           + TGDDCISLGDGSKRIKVNNVICGPGHGISIGSLGKYNNEEPVEGVIVNNCTIINTTNG
Sbjct: 215 IATGDDCISLGDGSKRIKVNNVICGPGHGISIGSLGKYNNEEPVEGVIVNNCTIINTTNG 274

Query: 161 VRIKTWPASPVAGIATNMHFSDITMVNVSNPILIDQEYCPWNQCNREIPSNIKINKVSFK 220
           VRIKTWPASPVAGIATNMHFSDITMVNVSNPILIDQEYCPWNQCNREIPSNIKINKVSFK
Sbjct: 275 VRIKTWPASPVAGIATNMHFSDITMVNVSNPILIDQEYCPWNQCNREIPSNIKINKVSFK 334

Query: 221 NIRGSSATPVAVKLICSSNLPCEEVKVANIDLVYNGIKGSITSECMNVKPIISGIQNPPI 280
           NIRGSSATPVAVKLICSSNLPCEEVKVANIDLVYNGIKGSITSECMNVKPIISGIQNPPI
Sbjct: 335 NIRGSSATPVAVKLICSSNLPCEEVKVANIDLVYNGIKGSITSECMNVKPIISGIQNPPI 394

Query: 281 CSSSYTTASK 291
           CSSSYTTASK
Sbjct: 395 CSSSYTTASK 404

[Close]

BLAST of Cucsa.181130 vs. NCBI nr
Match: gi|700188231|gb|KGN43464.1| (hypothetical protein Csa_7G037910 [Cucumis sativus])

HSP 1 Score: 218.8 bits (556), Expect = 1.3e-53
Identity = 99/100 (99.00%), Postives = 100/100 (100.00%), Query Frame = 1


Query: 1   ALKDAWNDACASDRPSAVVIPKGTFKVREGEFKGPCKSPIEFRVHGTLQAPKHPHGDSWI 60
           ALKDAWNDACASDRPSAVVIPKGTFKVREGEFKGPCKSPIEFRVHGTLQAPKHPHGDSWI
Sbjct: 49  ALKDAWNDACASDRPSAVVIPKGTFKVREGEFKGPCKSPIEFRVHGTLQAPKHPHGDSWI 108

Query: 61  TFAYVDRLRLSGGGVFDGQGKAGWEKNDCHKRIDCAKLPI 101
           TFAYVDR+RLSGGGVFDGQGKAGWEKNDCHKRIDCAKLPI
Sbjct: 109 TFAYVDRMRLSGGGVFDGQGKAGWEKNDCHKRIDCAKLPI 148

HSP 2 Score: 364.8 bits (935), Expect = 1.4e-97
Identity = 173/187 (92.51%), Postives = 179/187 (95.72%), Query Frame = 1


Query: 101 VTTGDDCISLGDGSKRIKVNNVICGPGHGISIGSLGKYNNEEPVEGVIVNNCTIINTTNG 160
           + TGDDCISLGDGSKR+K+ NV CGPGHGISIGSLGKY NEEPVEGVIV NCTI+NTTNG
Sbjct: 216 IATGDDCISLGDGSKRVKITNVTCGPGHGISIGSLGKYTNEEPVEGVIVKNCTIMNTTNG 275

Query: 161 VRIKTWPASPVAGIATNMHFSDITMVNVSNPILIDQEYCPWNQCNREIPSNIKINKVSFK 220
           VRIKTWPASPVAGIATNMHF DITMVNVSNPILIDQEYCPWNQCNR+IPS IKINKVSFK
Sbjct: 276 VRIKTWPASPVAGIATNMHFLDITMVNVSNPILIDQEYCPWNQCNRKIPSKIKINKVSFK 335

Query: 221 NIRGSSATPVAVKLICSSNLPCEEVKVANIDLVYNGIKGSITSECMNVKPIISGIQNPPI 280
           NIRGSSATPVAVKLICSSNLPCEEVKVANIDLVYNGIKGS+TSECMNVKPIISG QNPPI
Sbjct: 336 NIRGSSATPVAVKLICSSNLPCEEVKVANIDLVYNGIKGSLTSECMNVKPIISGTQNPPI 395

Query: 281 CSSSYTT 288
           CSSSYTT
Sbjct: 396 CSSSYTT 402

[Close]

BLAST of Cucsa.181130 vs. NCBI nr
Match: gi|659111053|ref|XP_008455553.1| (PREDICTED: exopolygalacturonase-like [Cucumis melo])

HSP 1 Score: 201.1 bits (510), Expect = 2.7e-48
Identity = 91/100 (91.00%), Postives = 94/100 (94.00%), Query Frame = 1


Query: 1   ALKDAWNDACASDRPSAVVIPKGTFKVREGEFKGPCKSPIEFRVHGTLQAPKHPHGDSWI 60
           ALKDAWNDACAS RPSAVVIPKGTFKVREGEFKGPCKSP+E RV GTLQAPKHPHGDSWI
Sbjct: 50  ALKDAWNDACASLRPSAVVIPKGTFKVREGEFKGPCKSPVEVRVRGTLQAPKHPHGDSWI 109

Query: 61  TFAYVDRLRLSGGGVFDGQGKAGWEKNDCHKRIDCAKLPI 101
           TFAYVDR+ LSGGGVFDGQGKAGWEKNDCHK I CA+LPI
Sbjct: 110 TFAYVDRMTLSGGGVFDGQGKAGWEKNDCHKHISCARLPI 149

HSP 2 Score: 321.2 bits (822), Expect = 1.8e-84
Identity = 158/301 (52.49%), Postives = 202/301 (67.11%), Query Frame = 1


Query: 5   AWNDACASDRPSAVVIPKGTFKVREGEFKGPCKSPIEFRVHGTLQAPKHPH----GDSWI 64
           AW  ACAS     +VIP GT+++   + KGPCK+PIE +V GT+QAP +P        W 
Sbjct: 2   AWTQACASPTAVKIVIPAGTYQMGAVDVKGPCKAPIEVQVDGTIQAPANPTDLKAAHQWF 61

Query: 65  TFAYVDRLRLSGGGVFDGQGKAGWEKNDCHKRIDCAKLPIVT------------------ 124
              YV+   LSG GVFDGQG   W++NDC    +C  L + T                  
Sbjct: 62  VVQYVNSFTLSGKGVFDGQGATAWKQNDCTTNKNCKMLCMPTKPHIGRSTDVKILNTNIA 121

Query: 125 TGDDCISLGDGSKRIKVNNVICGPGHGISIGSLGKYNNEEPVEGVIVNNCTIINTTNGVR 184
           TGDDC+SLGDG K I V NV CGPGHGIS+GSLGKY++EEPV G +V NCT+  T NGVR
Sbjct: 122 TGDDCVSLGDGCKNITVQNVNCGPGHGISVGSLGKYDSEEPVAGFLVKNCTLNGTDNGVR 181

Query: 185 IKTWPASPVAGIATNMHFSDITMVNVSNPILIDQEYCPWNQCNREIPSNIKINKVSFKNI 244
           IKTWP +P A   T+MHF D+TM NV+NPI+IDQEYCPWNQC+++ PS IKI+KVSFKNI
Sbjct: 182 IKTWPNTPGAITITDMHFEDLTMNNVTNPIIIDQEYCPWNQCSKQNPSKIKISKVSFKNI 241

Query: 245 RGSSATPVAVKLICSSNLPCEEVKVANIDLVYNGIKGSITSECMNVKPIISGIQNPPICS 284
           +G+S +   V L+CSS +PCE V++A+IDL +NG   + T++C NVKP I+G    P C+
Sbjct: 242 KGTSGSQEGVVLVCSSGVPCEGVEMADIDLTFNG--AAATAKCANVKPTITG--KAPTCA 298

[Close]

BLAST of Cucsa.181130 vs. NCBI nr
Match: gi|731409339|ref|XP_010657162.1| (PREDICTED: polygalacturonase [Vitis vinifera])

HSP 1 Score: 320.9 bits (821), Expect = 2.4e-84
Identity = 160/293 (54.61%), Postives = 199/293 (67.92%), Query Frame = 1


Query: 1   ALKDAWNDACASDRPSAVVIPKGTFKVREGEFKGPCKSPIEFRVHGTLQAP----KHPHG 60
           AL  AW DAC+S   S V+IP GT+ + +    GPCK+PI F V GT+ AP    +    
Sbjct: 46  ALLKAWGDACSSPVASTVMIPDGTYALGQIIIAGPCKAPINFVVQGTVMAPVDTSRFRAE 105

Query: 61  DSWITFAYVDRLRLSGGGVFDGQGKAGWEKNDCHKRIDCAKLPI-------VTTGDDCIS 120
             WITF  +D+  LSG GVFDGQGK  W    C     C +LPI         TGDDC+S
Sbjct: 106 AGWITFQQIDQFTLSGDGVFDGQGKIVWGTK-CLSSAYCNQLPINLRFNFITKTGDDCVS 165

Query: 121 LGDGSKRIKVNNVICGPGHGISIGSLGKYNNEEPVEGVIVNNCTIINTTNGVRIKTWPAS 180
           +GDGS++I +  V CGPGHGIS+GSLGKY NEEPV G+ V NCT+ NT NGVR+KTWPAS
Sbjct: 166 IGDGSEQINIQRVTCGPGHGISVGSLGKYPNEEPVVGISVKNCTLTNTQNGVRVKTWPAS 225

Query: 181 PVAGIATNMHFSDITMVNVSNPILIDQEYCPWNQCNREIPSNIKINKVSFKNIRGSSATP 240
              G A+ MHF DI M NV NPI+IDQEYCP NQCN + PS IKI+ VSF+NIRG+++T 
Sbjct: 226 H-QGTASEMHFEDIVMNNVGNPIIIDQEYCPHNQCNLKSPSRIKISNVSFRNIRGTTSTQ 285

Query: 241 VAVKLICSSNLPCEEVKVANIDLVYNGIKGSITSECMNVKPIISGIQNPPICS 283
           VAVKLICS  +PC++V++ +I+L YNG  G   S+C N+KP + G Q P IC+
Sbjct: 286 VAVKLICSQGVPCQDVELGDINLEYNGNDGPAMSQCKNIKPNVLGAQLPRICA 336

The following BLAST results are available for this feature:

BLAST of Cucsa.181130 vs. Swiss-Prot


Match Name	E-value	Identity	Description
PGLR_OENOR	1.8e-63	60.32	Exopolygalacturonase (Fragment) OS=Oenothera organensis PE=2 SV=1	[more]
PGLR_GOSHI	1.3e-61	58.79	Polygalacturonase OS=Gossypium hirsutum GN=G9 PE=2 SV=1	[more]
PGLR_GOSBA	6.3e-61	58.24	Polygalacturonase OS=Gossypium barbadense GN=G9 PE=2 SV=1	[more]
PGLR_TOBAC	7.7e-59	54.26	Polygalacturonase OS=Nicotiana tabacum GN=PG1 PE=2 SV=1	[more]
PGLR2_ARATH	1.7e-58	57.69	Exopolygalacturonase clone GBGA483 OS=Arabidopsis thaliana GN=At3g07850 PE=2 SV=...	[more]

BLAST of Cucsa.181130 vs. TrEMBL


Match Name	E-value	Identity	Description
A0A0A0K3B7_CUCSA	3.8e-105	98.95	Uncharacterized protein OS=Cucumis sativus GN=Csa_7G037910 PE=3 SV=1	[more]
A0A0A0K3B7_CUCSA	8.8e-54	99.00	Uncharacterized protein OS=Cucumis sativus GN=Csa_7G037910 PE=3 SV=1	[more]
A0A0B2SJ99_GLYSO	1.3e-84	52.49	Polygalacturonase (Fragment) OS=Glycine soja GN=glysoja_050108 PE=3 SV=1	[more]
A0A078G9V9_BRANA	1.1e-77	44.89	BnaC01g39620D protein OS=Brassica napus GN=BnaC01g39620D PE=3 SV=1	[more]
A0A0D3BA84_BRAOL	2.6e-77	44.58	Uncharacterized protein OS=Brassica oleracea var. oleracea PE=3 SV=1	[more]

BLAST of Cucsa.181130 vs. TAIR10


Match Name	E-value	Identity	Description
AT3G07840.1	6.7e-61	56.77	Pectin lyase-like superfamily protein	[more]
AT3G14040.1	1.9e-60	58.24	Pectin lyase-like superfamily protein	[more]
AT3G07820.1	3.3e-60	55.74	Pectin lyase-like superfamily protein	[more]
AT5G48140.1	7.4e-60	53.68	Pectin lyase-like superfamily protein	[more]
AT3G07850.1	9.6e-60	57.69	Pectin lyase-like superfamily protein	[more]

BLAST of Cucsa.181130 vs. NCBI nr


Match Name	E-value	Identity	Description
gi\|778730103\|ref\|XP_011659699.1\|	5.4e-105	98.95	PREDICTED: exopolygalacturonase-like [Cucumis sativus]	[more]
gi\|778730103\|ref\|XP_011659699.1\|	1.3e-53	99.00	PREDICTED: exopolygalacturonase-like [Cucumis sativus]	[more]
gi\|700188231\|gb\|KGN43464.1\|	1.3e-53	99.00	hypothetical protein Csa_7G037910 [Cucumis sativus]	[more]
gi\|659111053\|ref\|XP_008455553.1\|	2.7e-48	91.00	PREDICTED: exopolygalacturonase-like [Cucumis melo]	[more]
gi\|731409339\|ref\|XP_010657162.1\|	2.4e-84	54.61	PREDICTED: polygalacturonase [Vitis vinifera]	[more]

Annotated Terms

The following terms have been associated with this gene:

Vocabulary: **INTERPRO**
Term	Definition
IPR000743	Glyco_hydro_28
IPR006626	PbH1
IPR011050	Pectin_lyase_fold/virulence
IPR012334	Pectin_lyas_fold

Vocabulary: **Molecular Function**
Term	Definition
GO:0004650	polygalacturonase activity

Vocabulary: **Biological Process**
Term	Definition
GO:0005975	carbohydrate metabolic process

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0071555	cell wall organization
biological_process	GO:0005982	starch metabolic process
biological_process	GO:0005985	sucrose metabolic process
biological_process	GO:0005975	carbohydrate metabolic process
cellular_component	GO:0005576	extracellular region
molecular_function	GO:0004650	polygalacturonase activity

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Cucsa.181130.1	Cucsa.181130.1	mRNA

InterPro

Analysis Name: InterPro Annotations of cucumber (Gy14)
Date Performed: 2017-01-17


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR000743	Glycoside hydrolase, family 28	PFAM	PF00295	Glyco_hydro_28	coord: 100..269 score: 6.9
IPR000743	Glycoside hydrolase, family 28	PROSITE	PS00502	POLYGALACTURONASE	coord: 121..134 scor
IPR006626	Parallel beta-helix repeat	SMART	SM00710	pbh1	coord: 175..196 score: 3100.0coord: 114..134 score: 1700.0coord: 144..165 score:
IPR011050	Pectin lyase fold/virulence factor	unknown	SSF51126	Pectin lyase-like	coord: 1..286 score: 1.66
IPR012334	Pectin lyase fold	GENE3D	G3DSA:2.160.20.10		coord: 101..284 score: 4.6E-60coord: 1..96 score: 2.2
None	No IPR available	PANTHER	PTHR31375	FAMILY NOT NAMED	coord: 1..286 score: 1.9E
None	No IPR available	PANTHER	PTHR31375:SF37	PECTIN LYASE-LIKE SUPERFAMILY PROTEIN-RELATED	coord: 1..286 score: 1.9E

Synteny

The following gene(s) are orthologous to this gene:


Gene	Orthologue	Organism	Block
Cucsa.181130	MELO3C018881.2	Melon (DHL92) v3.6.1	cgymedB321
Cucsa.181130	MELO3C018871.2	Melon (DHL92) v3.6.1	cgymedB322
Cucsa.181130	CsaV3_7G003040	Cucumber (Chinese Long) v3	cgycucB292

The following gene(s) are paralogous to this gene:

None

The following block(s) are covering this gene:


Gene	Organism	Block
Cucsa.181130	Silver-seed gourd	carcgyB0243
Cucsa.181130	Silver-seed gourd	carcgyB1185
Cucsa.181130	Watermelon (97103) v2	cgywmbB326
Cucsa.181130	Wax gourd	cgywgoB371
Cucsa.181130	Cucurbita maxima (Rimu)	cgycmaB0515
Cucsa.181130	Cucurbita maxima (Rimu)	cgycmaB0516
Cucsa.181130	Cucurbita moschata (Rifu)	cgycmoB0515
Cucsa.181130	Cucurbita moschata (Rifu)	cgycmoB0516
Cucsa.181130	Wild cucumber (PI 183967)	cgycpiB287
Cucsa.181130	Cucumber (Chinese Long) v2	cgycuB277
Cucsa.181130	Melon (DHL92) v3.5.1	cgymeB327
Cucsa.181130	Watermelon (Charleston Gray)	cgywcgB322
Cucsa.181130	Watermelon (97103) v1	cgywmB346
Cucsa.181130	Cucurbita pepo (Zucchini)	cgycpeB0497
Cucsa.181130	Cucurbita pepo (Zucchini)	cgycpeB0498
Cucsa.181130	Bottle gourd (USVL1VR-Ls)	cgylsiB303