Cucsa.180180 (gene) Cucumber (Gy14) v1

BLAST of Cucsa.180180 vs. Swiss-Prot
Match: CCD4_ARATH (Probable carotenoid cleavage dioxygenase 4, chloroplastic OS=Arabidopsis thaliana GN=CCD4 PE=1 SV=1)

HSP 1 Score: 783.5 bits (2022), Expect = 1.6e-225
Identity = 388/600 (64.67%), Postives = 475/600 (79.17%), Query Frame = 1

Query: 1   MDSISSP-FLSGR-NLILSPPISSSLPPISTPIYSVLTEQNVKKNTPPPDADSPSPPLPR 60
           MDS+SS  FLS   +L  S     S  P    I S + E+      P  + D  + P   
Sbjct: 1   MDSVSSSSFLSSTFSLHHSLLRRRSSSPTLLRINSAVVEERSPITNPSDNNDRRNKPKTL 60

Query: 61  PSPPSPPMPRVSSTRRVQP--SLPARFFNAFDDLINNFINPPVSPSVDPRYILADNFAPV 120
            +  +  +  VSS  +++P  +L    F   +D+IN FI+PP  PSVDP+++L+DNFAPV
Sbjct: 61  HNRTNHTL--VSSPPKLRPEMTLATALFTTVEDVINTFIDPPSRPSVDPKHVLSDNFAPV 120

Query: 121 -DELPPTECEVIYGSLPSSLNGAYIRNGPNPQYLPRGPYHLFDGDGMLHSLRISDGRAVL 180
            DELPPT+CE+I+G+LP SLNGAYIRNGPNPQ+LPRGPYHLFDGDGMLH+++I +G+A L
Sbjct: 121 LDELPPTDCEIIHGTLPLSLNGAYIRNGPNPQFLPRGPYHLFDGDGMLHAIKIHNGKATL 180

Query: 181 CSRYVKTYKYTLERDAGHPVFPNVFSGFNGLTASAARGAVAVGRILTGQYNPANGIGLAN 240
           CSRYVKTYKY +E+  G PV PNVFSGFNG+TAS ARGA+   R+LTGQYNP NGIGLAN
Sbjct: 181 CSRYVKTYKYNVEKQTGAPVMPNVFSGFNGVTASVARGALTAARVLTGQYNPVNGIGLAN 240

Query: 241 TSLAFFGDRLYALGESDLPYPIRLTPNGDIETLARHDFDGKLTLSMTAHPKVDSDTGEAF 300
           TSLAFF +RL+ALGESDLPY +RLT +GDIET+ R+DFDGKL +SMTAHPK D  TGE F
Sbjct: 241 TSLAFFSNRLFALGESDLPYAVRLTESGDIETIGRYDFDGKLAMSMTAHPKTDPITGETF 300

Query: 301 AFRYGPLPPFLTYFRFDKNGAKHSDVPILSMNRPSFLHDFAITKKYAVFTDIQIG--INP 360
           AFRYGP+PPFLTYFRFD  G K  DVPI SM  PSFLHDFAITK++A+F +IQ+G  +N 
Sbjct: 301 AFRYGPVPPFLTYFRFDSAGKKQRDVPIFSMTSPSFLHDFAITKRHAIFAEIQLGMRMNM 360

Query: 361 TQMIIEGGSPVGSDPSKISRVGLIPRYANDESKMKWFDVPGLNLIHAINAWDEDD--AVV 420
             +++EGGSPVG+D  K  R+G+IP+YA DES+MKWF+VPG N+IHAINAWDEDD  +VV
Sbjct: 361 LDLVLEGGSPVGTDNGKTPRLGVIPKYAGDESEMKWFEVPGFNIIHAINAWDEDDGNSVV 420

Query: 421 IVAPNILSVEHALERMDLVHALVEKIRIDLKTGIVTRTPLSTRNLDFGVIHPSYVGKKHR 480
           ++APNI+S+EH LERMDLVHALVEK++IDL TGIV R P+S RNLDF VI+P+++G+  R
Sbjct: 421 LIAPNIMSIEHTLERMDLVHALVEKVKIDLVTGIVRRHPISARNLDFAVINPAFLGRCSR 480

Query: 481 FVYAGVGDPMPKISGVVKLEISQEERRDCIVACRIFGPGCYGGEPFFVPRERESSDETEA 540
           +VYA +GDPMPKISGVVKL++S+ +R DC VA R++G GCYGGEPFFV R+       EA
Sbjct: 481 YVYAAIGDPMPKISGVVKLDVSKGDRDDCTVARRMYGSGCYGGEPFFVARD---PGNPEA 540

Query: 541 EEDDGYVVSYVHDENSGESRFIVMDAKSPELEIIAAVKLPRRVPYGFHGLFVKESDLNKL 592
           EEDDGYVV+YVHDE +GES+F+VMDAKSPELEI+AAV+LPRRVPYGFHGLFVKESDLNKL
Sbjct: 541 EEDDGYVVTYVHDEVTGESKFLVMDAKSPELEIVAAVRLPRRVPYGFHGLFVKESDLNKL 595

BLAST of Cucsa.180180 vs. Swiss-Prot
Match: ZCD_CROSA (Zeaxanthin 7,8(7',8')-cleavage dioxygenase, chromoplastic OS=Crocus sativus GN=ZCD PE=1 SV=1)

HSP 1 Score: 429.9 bits (1104), Expect = 4.6e-119
Identity = 216/369 (58.54%), Postives = 272/369 (73.71%), Query Frame = 1

Query: 224 QYNPANGIGLANTSLAFFGDRLYALGESDLPYPIRLTP-NGDIETLARHDFDGKLTLSMT 283
           Q +P  GIGLANTSL F   RL+AL E DLPY +RL+P +GDI T+ R + +   T S T
Sbjct: 2   QVDPTKGIGLANTSLQFSNGRLHALCEYDLPYVVRLSPEDGDISTVGRIE-NNVSTKSTT 61

Query: 284 AHPKVDSDTGEAFAFRYGPLPPFLTYFRFDKNGAKHS-DVPILSMNRPSFLHDFAITKKY 343
           AHPK D  TGE F+F YGP+ P++TY R+D +G K   DVPI S   PSF+HDFAIT+ Y
Sbjct: 62  AHPKTDPVTGETFSFSYGPIQPYVTYSRYDCDGKKSGPDVPIFSFKEPSFVHDFAITEHY 121

Query: 344 AVFTDIQIGINPTQMIIEGGSPVGSDPSKISRVGLIPRYANDESKMKWFDVPGLNLIHAI 403
           AVF DIQI + P + I+ G   +G D  K+ R+GL+PRYA  +S+M+WFDVPG N++H +
Sbjct: 122 AVFPDIQIVMKPAE-IVRGRRMIGPDLEKVPRLGLLPRYATSDSEMRWFDVPGFNMVHVV 181

Query: 404 NAWDED--DAVVIVAPNILSVEHALERMDLVHALVEKIRIDLKTGIVTRTPLSTRNLDFG 463
           NAW+E+  + VVIVAPN+  +E+A++R DL+H  VE  RI+LK+G V+RT LS  NLDFG
Sbjct: 182 NAWEEEGGEVVVIVAPNVSPIENAIDRFDLLHVSVEMARIELKSGSVSRTLLSAENLDFG 241

Query: 464 VIHPSYVGKKHRFVYAGVGDPMPKISGVVKLEISQEERRDCIVACRIFGPGCYGGEPFFV 523
           VIH  Y G+K R+ Y GVGDPMPKI GVVK++     R +C+VA R FG GC+GGEPFFV
Sbjct: 242 VIHRGYSGRKSRYAYLGVGDPMPKIRGVVKVDFELAGRGECVVARREFGVGCFGGEPFFV 301

Query: 524 PRERESSDETEAEEDDGYVVSYVHDENSGESRFIVMDAKSPELEIIAAVKLPRRVPYGFH 583
           P    SS ++  EEDDGYVVSY+HDE  GES F+VMDA+SPELEI+A V LPRRVPYGFH
Sbjct: 302 P---ASSKKSGGEEDDGYVVSYLHDEGKGESSFVVMDARSPELEILAEVVLPRRVPYGFH 361

Query: 584 GLFVKESDL 589
           GLFV E++L
Sbjct: 362 GLFVTEAEL 365

BLAST of Cucsa.180180 vs. Swiss-Prot
Match: NCED3_ARATH (9-cis-epoxycarotenoid dioxygenase NCED3, chloroplastic OS=Arabidopsis thaliana GN=NCED3 PE=2 SV=1)

HSP 1 Score: 384.4 bits (986), Expect = 2.2e-105
Identity = 228/611 (37.32%), Postives = 339/611 (55.48%), Query Frame = 1

Query: 3   SISSPFLSGRNLILSPPISSSLPP-----ISTPIYSVLTEQ-NVKKNTPPPDADSPSPPL 62
           ++S  +L G +    PP+SSS         S P+ S +T + NV      P    P+   
Sbjct: 9   AVSGRWLGGNHT--QPPLSSSQSSDLSYCSSLPMASRVTRKLNVSSALHTP----PALHF 68

Query: 63  PRPSPPSPPM---PRVSSTRRVQPSLPARFFNAFDDLINNFINP-----PVSPSVDPRYI 122
           P+ S  SP +   P+   +   Q +L  R   A  D    F+       P+  + DP   
Sbjct: 69  PKQSSNSPAIVVKPKAKESNTKQMNLFQRAAAAALDAAEGFLVSHEKLHPLPKTADPSVQ 128

Query: 123 LADNFAPVDELPPTECEVIYGSLPSSLNGAYIRNGPNPQYLPRGPYHLFDGDGMLHSLRI 182
           +A NFAPV+E P      + G LP S+ G Y+RNG NP + P   +H FDGDGM+H+++ 
Sbjct: 129 IAGNFAPVNEQPVRRNLPVVGKLPDSIKGVYVRNGANPLHEPVTGHHFFDGDGMVHAVKF 188

Query: 183 SDGRAVLCSRYVKTYKYTLERDAGHPVFPNVFSGFNGLTASAARGAVAVGRILTGQYNPA 242
             G A    R+ +T ++  ER  G PVFP      +G T   AR  +   R   G  +PA
Sbjct: 189 EHGSASYACRFTQTNRFVQERQLGRPVFPKAIGELHGHT-GIARLMLFYARAAAGIVDPA 248

Query: 243 NGIGLANTSLAFFGDRLYALGESDLPYPIRLTPNGDIETLARHDFDGKLTLSMTAHPKVD 302
           +G G+AN  L +F  RL A+ E DLPY +++TPNGD++T+ R DFDG+L  +M AHPKVD
Sbjct: 249 HGTGVANAGLVYFNGRLLAMSEDDLPYQVQITPNGDLKTVGRFDFDGQLESTMIAHPKVD 308

Query: 303 SDTGEAFAFRYGPL-PPFLTYFRFDKNGAKHSDVPILSMNRPSFLHDFAITKKYAVFTDI 362
            ++GE FA  Y  +  P+L YFRF  +G K  DV I  +++P+ +HDFAIT+ + V  D 
Sbjct: 309 PESGELFALSYDVVSKPYLKYFRFSPDGTKSPDVEI-QLDQPTMMHDFAITENFVVVPDQ 368

Query: 363 QIGINPTQMIIEGGSPVGSDPSKISRVGLIPRYANDESKMKWFDVPGLNLIHAINAWD-- 422
           Q+     +M I GGSPV  D +K++R G++ +YA D S +KW D P     H  NAW+  
Sbjct: 369 QVVFKLPEM-IRGGSPVVYDKNKVARFGILDKYAEDSSNIKWIDAPDCFCFHLWNAWEEP 428

Query: 423 EDDAVVIVAPNILSVEHALERMD-LVHALVEKIRIDLKTGIVTRTPLSTR-----NLDFG 482
           E D VV++   +   +      D  + +++ +IR++LKTG  TR P+ +      NL+ G
Sbjct: 429 ETDEVVVIGSCMTPPDSIFNESDENLKSVLSEIRLNLKTGESTRRPIISNEDQQVNLEAG 488

Query: 483 VIHPSYVGKKHRFVYAGVGDPMPKISGVVKLEISQEERRDCIVACRIFGPGCYGGEPFFV 542
           +++ + +G+K +F Y  + +P PK+SG  K++++  E     V   ++G   YGGEP F+
Sbjct: 489 MVNRNMLGRKTKFAYLALAEPWPKVSGFAKVDLTTGE-----VKKHLYGDNRYGGEPLFL 548

Query: 543 PRERESSDETEAEEDDGYVVSYVHDENSGESRFIVMDAKSPELEIIAAVKLPRRVPYGFH 591
           P E         EED+GY++ +VHDE + +S   +++A S  LE+ A VKLP RVPYGFH
Sbjct: 549 PGE-------GGEEDEGYILCFVHDEKTWKSELQIVNAVS--LEVEATVKLPSRVPYGFH 596

BLAST of Cucsa.180180 vs. Swiss-Prot
Match: NCED_ONCHC (9-cis-epoxycarotenoid dioxygenase, chloroplastic OS=Oncidium hybrid cultivar GN=NCED PE=2 SV=1)

HSP 1 Score: 377.1 bits (967), Expect = 3.5e-103
Identity = 219/566 (38.69%), Postives = 326/566 (57.60%), Query Frame = 1

Query: 48  DADSPSPPLPRPSPPSPPMPR-VSSTRRVQPSLPARFFNAF------------DDLINNF 107
           D  SPSP  P P    P  P+ +   +  +PS P+  +N F            D+LI N 
Sbjct: 64  DTTSPSPFYPLP----PTCPKEIHPEQSAKPSRPS--WNLFQRAAAAVLEAVEDNLIQNL 123

Query: 108 INP--PVSPSVDPRYILADNFAPVDELPPTECEVIYGSLPSSLNGAYIRNGPNPQYLPRG 167
           +    P+  + DP   +A NFAPV E  P     + G +P  +NG Y+RNG NP + P  
Sbjct: 124 LESGHPLPKTADPAVQIAGNFAPVGEQKPHHDLPVDGRIPPLINGVYLRNGANPLFEPVA 183

Query: 168 PYHLFDGDGMLHSLRISDGRAVLCSRYVKTYKYTLERDAGHPVFPNVFSGFNGLTASAAR 227
            +H FDGDGM+H++ + +GRA    R+ +T +   ER  G  +FP      +G  +  AR
Sbjct: 184 GHHFFDGDGMVHAVHLRNGRASYACRFTETERLKQERAVGRAIFPKAIGELHG-HSGIAR 243

Query: 228 GAVAVGRILTGQYNPANGIGLANTSLAFFGDRLYALGESDLPYPIRLTPNGDIETLARHD 287
             +   R L G  + + G G+AN  L +F +RL A+ E DLPY +R+ PNGD+ET  R+D
Sbjct: 244 LLLFYARGLLGLIDHSRGTGVANAGLIYFNNRLLAMSEDDLPYHVRIKPNGDLETAGRYD 303

Query: 288 FDGKLTLSMTAHPKVDSDTGEAFAFRYGPL-PPFLTYFRFDKNGAKHSDVPILSMNRPSF 347
           FDG+LT +M AHPK+D +T E FA  Y  +  P+L YFRF   G K  DV I  + +P+ 
Sbjct: 304 FDGQLTTTMIAHPKLDPETREFFALSYDVIKKPYLKYFRFSPCGEKSPDVEI-PLPQPTM 363

Query: 348 LHDFAITKKYAVFTDIQIGINPTQMIIEGGSPVGSDPSKISRVGLIPRYANDESKMKWFD 407
           +HDFAITK + +  D Q+     +MI  GGSPV  D  KI+R G++P+YA D S+M+W D
Sbjct: 364 MHDFAITKNFVIIPDQQVVFKLQEMIC-GGSPVVYDKEKIARFGVLPKYAIDASEMQWID 423

Query: 408 VPGLNLIHAINAWDEDDA-VVIVAPNILSVEHAL--ERMDLVHALVEKIRIDLKTGIVTR 467
           VP     H  N+W+E +   V+V  + ++   ++  E  + + +++ +IR++L+TG  TR
Sbjct: 424 VPDCFCFHLWNSWEEPETEEVVVIGSCMTPPDSIFNESEENLQSVLTEIRLNLRTGKSTR 483

Query: 468 TPL-----STRNLDFGVIHPSYVGKKHRFVYAGVGDPMPKISGVVKLEISQEERRDCIVA 527
            P+     S  NL+ G+++ + +G++ RF Y  + +P PK+SG  K++++  E     + 
Sbjct: 484 RPILRPGNSQINLEAGMVNRNRLGRRTRFAYLAIAEPWPKVSGFAKVDLASGE-----IQ 543

Query: 528 CRIFGPGCYGGEPFFVPRERESSDETEAEEDDGYVVSYVHDENSGESRFIVMDAKSPELE 587
              +G G YGGEP+FVP  RE  D     ED GYV+++VHDE  G S  ++M+A    LE
Sbjct: 544 RFEYGDGGYGGEPYFVP--REGCD----REDGGYVLAFVHDEREGSSELLIMNAADMRLE 603

Query: 588 IIAAVKLPRRVPYGFHGLFVKESDLN 590
             AAV+LP RVPYGF+G FV  ++L+
Sbjct: 604 --AAVRLPSRVPYGFYGTFVSATELH 607

BLAST of Cucsa.180180 vs. Swiss-Prot
Match: NCED5_ARATH (Probable 9-cis-epoxycarotenoid dioxygenase NCED5, chloroplastic OS=Arabidopsis thaliana GN=NCED5 PE=1 SV=1)

HSP 1 Score: 374.8 bits (961), Expect = 1.8e-102
Identity = 220/582 (37.80%), Postives = 325/582 (55.84%), Query Frame = 1

Query: 19  PISSSLPPISTPIYSVLTEQNVKKNTPPPDADSPS----PPLPRPSPPSPPMPRVSSTRR 78
           P     P  S+ +    T+   +K +    +D+P+    P  P P+P  P     S    
Sbjct: 20  PSDLDAPSPSSSVSFTNTKPRRRKLSANSVSDTPNLLNFPNYPSPNPIIPEKD-TSRWNP 79

Query: 79  VQPSLPARFFNAFDDLINNFINPPVSPSVDPRYILADNFAPVDELPPTECEVIYGSLPSS 138
           +Q +  A    A   L+    + P+  +VDPR+ ++ N+APV E        + G +P  
Sbjct: 80  LQRAASAALDFAETALLRRERSKPLPKTVDPRHQISGNYAPVPEQSVKSSLSVDGKIPDC 139

Query: 139 LNGAYIRNGPNPQYLPRGPYHLFDGDGMLHSLRISDGRAVLCSRYVKTYKYTLERDAGHP 198
           ++G Y+RNG NP + P   +HLFDGDGM+H+++I++G A    R+ +T +   E+  G P
Sbjct: 140 IDGVYLRNGANPLFEPVSGHHLFDGDGMVHAVKITNGDASYSCRFTETERLVQEKQLGSP 199

Query: 199 VFPNVFSGFNGLTASAARGAVAVGRILTGQYNPANGIGLANTSLAFFGDRLYALGESDLP 258
           +FP      +G  +  AR  +   R L G  N  NG G+AN  L +F DRL A+ E DLP
Sbjct: 200 IFPKAIGELHG-HSGIARLMLFYARGLFGLLNHKNGTGVANAGLVYFHDRLLAMSEDDLP 259

Query: 259 YPIRLTPNGDIETLARHDFDGKLTLSMTAHPKVDSDTGEAFAFRYGPL-PPFLTYFRFDK 318
           Y +R+T NGD+ET+ R DFDG+L+ +M AHPK+D  T E FA  Y  +  P+L YF+F  
Sbjct: 260 YQVRVTDNGDLETIGRFDFDGQLSSAMIAHPKIDPVTKELFALSYDVVKKPYLKYFKFSP 319

Query: 319 NGAKHSDVPILSMNRPSFLHDFAITKKYAVFTDIQIGINPTQMIIEGGSPVGSDPSKISR 378
            G K  DV I  +  P+ +HDFAIT+ + V  D Q+    + M + G SPV  D  KISR
Sbjct: 320 EGEKSPDVEI-PLASPTMMHDFAITENFVVIPDQQVVFKLSDMFL-GKSPVKYDGEKISR 379

Query: 379 VGLIPRYANDESKMKWFDVPGLNLIHAINAWD--EDDAVVIVAPNILSVEHALERMD-LV 438
            G++PR A D S+M W + P     H  NAW+  E D VV++   +   +      D  +
Sbjct: 380 FGILPRNAKDASEMVWVESPETFCFHLWNAWESPETDEVVVIGSCMTPADSIFNECDEQL 439

Query: 439 HALVEKIRIDLKTGIVTRTPL----STRNLDFGVIHPSYVGKKHRFVYAGVGDPMPKISG 498
           ++++ +IR++LKTG  TR  +       NL+ G+++ + +G+K R+ Y  + +P PK+SG
Sbjct: 440 NSVLSEIRLNLKTGKSTRRTIIPGSVQMNLEAGMVNRNLLGRKTRYAYLAIAEPWPKVSG 499

Query: 499 VVKLEISQEERRDCIVACRIFGPGCYGGEPFFVPRERESSDETEAEEDDGYVVSYVHDEN 558
             K+++S  E     V    +G   YGGEPFF+PR  ES       EDDGY++S+VHDE 
Sbjct: 500 FAKVDLSTGE-----VKNHFYGGKKYGGEPFFLPRGLESDG-----EDDGYIMSFVHDEE 559

Query: 559 SGESRFIVMDAKSPELEIIAAVKLPRRVPYGFHGLFVKESDL 589
           S ES   +++A + ELE  A VKLP RVPYGFHG FV  +D+
Sbjct: 560 SWESELHIVNAVTLELE--ATVKLPSRVPYGFHGTFVNSADM 585

BLAST of Cucsa.180180 vs. TrEMBL
Match: A0A0A0KV99_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_4G056640 PE=4 SV=1)

HSP 1 Score: 1205.3 bits (3117), Expect = 0.0e+00
Identity = 591/591 (100.00%), Postives = 591/591 (100.00%), Query Frame = 1

Query: 1   MDSISSPFLSGRNLILSPPISSSLPPISTPIYSVLTEQNVKKNTPPPDADSPSPPLPRPS 60
           MDSISSPFLSGRNLILSPPISSSLPPISTPIYSVLTEQNVKKNTPPPDADSPSPPLPRPS
Sbjct: 1   MDSISSPFLSGRNLILSPPISSSLPPISTPIYSVLTEQNVKKNTPPPDADSPSPPLPRPS 60

Query: 61  PPSPPMPRVSSTRRVQPSLPARFFNAFDDLINNFINPPVSPSVDPRYILADNFAPVDELP 120
           PPSPPMPRVSSTRRVQPSLPARFFNAFDDLINNFINPPVSPSVDPRYILADNFAPVDELP
Sbjct: 61  PPSPPMPRVSSTRRVQPSLPARFFNAFDDLINNFINPPVSPSVDPRYILADNFAPVDELP 120

Query: 121 PTECEVIYGSLPSSLNGAYIRNGPNPQYLPRGPYHLFDGDGMLHSLRISDGRAVLCSRYV 180
           PTECEVIYGSLPSSLNGAYIRNGPNPQYLPRGPYHLFDGDGMLHSLRISDGRAVLCSRYV
Sbjct: 121 PTECEVIYGSLPSSLNGAYIRNGPNPQYLPRGPYHLFDGDGMLHSLRISDGRAVLCSRYV 180

Query: 181 KTYKYTLERDAGHPVFPNVFSGFNGLTASAARGAVAVGRILTGQYNPANGIGLANTSLAF 240
           KTYKYTLERDAGHPVFPNVFSGFNGLTASAARGAVAVGRILTGQYNPANGIGLANTSLAF
Sbjct: 181 KTYKYTLERDAGHPVFPNVFSGFNGLTASAARGAVAVGRILTGQYNPANGIGLANTSLAF 240

Query: 241 FGDRLYALGESDLPYPIRLTPNGDIETLARHDFDGKLTLSMTAHPKVDSDTGEAFAFRYG 300
           FGDRLYALGESDLPYPIRLTPNGDIETLARHDFDGKLTLSMTAHPKVDSDTGEAFAFRYG
Sbjct: 241 FGDRLYALGESDLPYPIRLTPNGDIETLARHDFDGKLTLSMTAHPKVDSDTGEAFAFRYG 300

Query: 301 PLPPFLTYFRFDKNGAKHSDVPILSMNRPSFLHDFAITKKYAVFTDIQIGINPTQMIIEG 360
           PLPPFLTYFRFDKNGAKHSDVPILSMNRPSFLHDFAITKKYAVFTDIQIGINPTQMIIEG
Sbjct: 301 PLPPFLTYFRFDKNGAKHSDVPILSMNRPSFLHDFAITKKYAVFTDIQIGINPTQMIIEG 360

Query: 361 GSPVGSDPSKISRVGLIPRYANDESKMKWFDVPGLNLIHAINAWDEDDAVVIVAPNILSV 420
           GSPVGSDPSKISRVGLIPRYANDESKMKWFDVPGLNLIHAINAWDEDDAVVIVAPNILSV
Sbjct: 361 GSPVGSDPSKISRVGLIPRYANDESKMKWFDVPGLNLIHAINAWDEDDAVVIVAPNILSV 420

Query: 421 EHALERMDLVHALVEKIRIDLKTGIVTRTPLSTRNLDFGVIHPSYVGKKHRFVYAGVGDP 480
           EHALERMDLVHALVEKIRIDLKTGIVTRTPLSTRNLDFGVIHPSYVGKKHRFVYAGVGDP
Sbjct: 421 EHALERMDLVHALVEKIRIDLKTGIVTRTPLSTRNLDFGVIHPSYVGKKHRFVYAGVGDP 480

Query: 481 MPKISGVVKLEISQEERRDCIVACRIFGPGCYGGEPFFVPRERESSDETEAEEDDGYVVS 540
           MPKISGVVKLEISQEERRDCIVACRIFGPGCYGGEPFFVPRERESSDETEAEEDDGYVVS
Sbjct: 481 MPKISGVVKLEISQEERRDCIVACRIFGPGCYGGEPFFVPRERESSDETEAEEDDGYVVS 540

Query: 541 YVHDENSGESRFIVMDAKSPELEIIAAVKLPRRVPYGFHGLFVKESDLNKL 592
           YVHDENSGESRFIVMDAKSPELEIIAAVKLPRRVPYGFHGLFVKESDLNKL
Sbjct: 541 YVHDENSGESRFIVMDAKSPELEIIAAVKLPRRVPYGFHGLFVKESDLNKL 591

BLAST of Cucsa.180180 vs. TrEMBL
Match: K4JYD3_MOMCH (Carotenoid cleavage dioxygenase 4 OS=Momordica charantia GN=CCD4 PE=2 SV=1)

HSP 1 Score: 991.9 bits (2563), Expect = 3.4e-286
Identity = 496/597 (83.08%), Postives = 532/597 (89.11%), Query Frame = 1

Query: 1   MDSISSPFLSGRNLILSPPISSSLPPISTPIYSVLTEQN---VKKNTP-PPDADSPSPPL 60
           MD+ISSPFLSG NL+LSP IS S P I+T I SVLTE+    VKK  P P DADS SP  
Sbjct: 1   MDAISSPFLSGGNLLLSPAISISRPSIATVISSVLTEETPKTVKKTGPRPSDADS-SPLR 60

Query: 61  PRPSPPS--PPMPRVSSTRRVQPSLPARFFNAFDDLINNFINPPVSPSVDPRYILADNFA 120
             P PP+  P M + SSTRRV+PSLPAR FNAFDDLINNFINPPV+PSVDPRY+LADNFA
Sbjct: 61  ATPPPPAKVPAMAKSSSTRRVEPSLPARLFNAFDDLINNFINPPVNPSVDPRYVLADNFA 120

Query: 121 PVDELPPTECEVIYGSLPSSLNGAYIRNGPNPQYLPRGPYHLFDGDGMLHSLRISDGRAV 180
           PVDELPPTECEVI GSLP SLNGAYIRNGPNPQYLPRGPYHLFDGDGMLHSLRISDGRAV
Sbjct: 121 PVDELPPTECEVIQGSLPPSLNGAYIRNGPNPQYLPRGPYHLFDGDGMLHSLRISDGRAV 180

Query: 181 LCSRYVKTYKYTLERDAGHPVFPNVFSGFNGLTASAARGAVAVGRILTGQYNPANGIGLA 240
           LCSRYVKTYKYTLERD GHPV PNVFSGFNGLTASAAR AV  GR+LTGQ++PANGIGLA
Sbjct: 181 LCSRYVKTYKYTLERDVGHPVIPNVFSGFNGLTASAARSAVTAGRMLTGQFDPANGIGLA 240

Query: 241 NTSLAFFGDRLYALGESDLPYPIRLTPNGDIETLARHDFDGKLTLSMTAHPKVDSDTGEA 300
           NTSLA+FGDRLYALGESDLPYPIRLTP GDIETL RHDFDGKL++SMTAHPKVD  TGEA
Sbjct: 241 NTSLAYFGDRLYALGESDLPYPIRLTPTGDIETLDRHDFDGKLSISMTAHPKVDPVTGEA 300

Query: 301 FAFRYGPLPPFLTYFRFDKNGAKHSDVPILSMNRPSFLHDFAITKKYAVFTDIQIGINPT 360
           FAFRYGPLPPFLT+FRFD++GAK SDVPI SM+RPSFLHDFAIT+KYAVF + QIG NP 
Sbjct: 301 FAFRYGPLPPFLTFFRFDRSGAKQSDVPIFSMSRPSFLHDFAITEKYAVFGETQIGFNPM 360

Query: 361 QMIIEGGSPVGSDPSKISRVGLIPRYANDESKMKWFDVPGLNLIHAINAWDEDDAVVIVA 420
           QMI EG SPVGS+PSK+ R G+IPRYA DESKMKWFDVPG NLIHAINAWDE DAVV+VA
Sbjct: 361 QMITEGRSPVGSNPSKVCRAGIIPRYATDESKMKWFDVPGFNLIHAINAWDEYDAVVMVA 420

Query: 421 PNILSVEHALERMDLVHALVEKIRIDLKTGIVTRTPLSTRNLDFGVIHPSYVGKKHRFVY 480
           PNILSVEH +ERMDLVHALVEK RIDLKTGIVTR  LSTRNLDFGVI+PSYVG+K+RFVY
Sbjct: 421 PNILSVEHTMERMDLVHALVEKARIDLKTGIVTRRSLSTRNLDFGVINPSYVGRKNRFVY 480

Query: 481 AGVGDPMPKISGVVKLEISQEERRDCIVACRIFGPGCYGGEPFFVPRERESSDETEAEED 540
           AGVGDPMPKISGVVKL++SQEE RDCIVA RIFGPGCYGGEPF VPRE E++ ET AEE 
Sbjct: 481 AGVGDPMPKISGVVKLDVSQEECRDCIVASRIFGPGCYGGEPFLVPREGENAGETAAEEG 540

Query: 541 DGYVVSYVHDENSGESRFIVMDAKSPELEIIAAVKLPRRVPYGFHGLFVKESDLNKL 592
           DGYVVSYVH+EN+GESRFIVMDAKSP L I+AAVKLPRRVPYGF GLFVKESDLNKL
Sbjct: 541 DGYVVSYVHNENTGESRFIVMDAKSPNLAIVAAVKLPRRVPYGFLGLFVKESDLNKL 596

BLAST of Cucsa.180180 vs. TrEMBL
Match: S4UMV5_PRUPE (Carotenoid cleavage dioxygenase 4 OS=Prunus persica GN=ccd4 PE=4 SV=1)

HSP 1 Score: 878.2 bits (2268), Expect = 5.5e-252
Identity = 424/608 (69.74%), Postives = 500/608 (82.24%), Query Frame = 1

Query: 1   MDSISSPFLSG---RNLILSPPISSSLPPISTPIYSVLTEQNVKKNTPPPDADSPSPPLP 60
           MD+ SS FLS    +NL LSP I+       TP +S+ + +  ++ + PP A  P+    
Sbjct: 1   MDAFSSSFLSTFPTQNLSLSPAIA-------TPKFSISSVRIEERPSSPPPASKPTST-K 60

Query: 61  RPSPPSPPMPRVSSTRR--------------VQPSLPARFFNAFDDLINNFINPPVSPSV 120
            P PP  P P +++  R                P+LPA  FNA DD+INNFI+PP+ PSV
Sbjct: 61  APQPPKTPSPPLTTKARDYNNASTFSAAKKGTDPTLPAVIFNALDDIINNFIDPPLRPSV 120

Query: 121 DPRYILADNFAPVDELPPTECEVIYGSLPSSLNGAYIRNGPNPQYLPRGPYHLFDGDGML 180
           DP+++L++NFAPVDELPPTECE+I GSLP  L+GAYIRNGPNPQYLPRGPYHLFDGDGML
Sbjct: 121 DPKHVLSNNFAPVDELPPTECEIIQGSLPPCLDGAYIRNGPNPQYLPRGPYHLFDGDGML 180

Query: 181 HSLRISDGRAVLCSRYVKTYKYTLERDAGHPVFPNVFSGFNGLTASAARGAVAVGRILTG 240
           HS+RIS GRAVLCSRYVKTYKYT+ERDAG+P+ P+VFSGFNGLTASA RGA++  R+ TG
Sbjct: 181 HSVRISKGRAVLCSRYVKTYKYTIERDAGYPILPSVFSGFNGLTASATRGALSAARVFTG 240

Query: 241 QYNPANGIGLANTSLAFFGDRLYALGESDLPYPIRLTPNGDIETLARHDFDGKLTLSMTA 300
           QYNPANGIGLANTSLAFFG++LYALGESDLPY +RLT NGDI+TL RHDFDGKL +SMTA
Sbjct: 241 QYNPANGIGLANTSLAFFGNQLYALGESDLPYSLRLTSNGDIQTLGRHDFDGKLFMSMTA 300

Query: 301 HPKVDSDTGEAFAFRYGPLPPFLTYFRFDKNGAKHSDVPILSMNRPSFLHDFAITKKYAV 360
           HPK+D +TGEAFAFRYGPLPPFLTYFRFD NG K  DVPI SM  PSFLHDFAITKKYA+
Sbjct: 301 HPKIDPETGEAFAFRYGPLPPFLTYFRFDANGTKQPDVPIFSMVTPSFLHDFAITKKYAI 360

Query: 361 FTDIQIGINPTQMIIEGGSPVGSDPSKISRVGLIPRYANDESKMKWFDVPGLNLIHAINA 420
           F DIQIG+NP  MI +G SPVG DPSK+ R+G+IPRYA DE++M+WFDVPG N+IHAINA
Sbjct: 361 FVDIQIGMNPIDMITKGASPVGLDPSKVPRIGVIPRYAKDETEMRWFDVPGFNIIHAINA 420

Query: 421 WDEDDAVVIVAPNILSVEHALERMDLVHALVEKIRIDLKTGIVTRTPLSTRNLDFGVIHP 480
           WDE+DA+V+VAPNILS EH +ERMDL+HA VEK+RIDLKTGIV+R P+STRNLDF V +P
Sbjct: 421 WDEEDAIVMVAPNILSAEHTMERMDLIHASVEKVRIDLKTGIVSRQPISTRNLDFAVFNP 480

Query: 481 SYVGKKHRFVYAGVGDPMPKISGVVKLEISQEERRDCIVACRIFGPGCYGGEPFFVPRER 540
           +YVGKK+++VYA VGDPMPKISGVVKL++S  E ++CIVA R+FGPGCYGGEPFFV RE 
Sbjct: 481 AYVGKKNKYVYAAVGDPMPKISGVVKLDVSNVEHKECIVASRMFGPGCYGGEPFFVAREP 540

Query: 541 ESSDETEAEEDDGYVVSYVHDENSGESRFIVMDAKSPELEIIAAVKLPRRVPYGFHGLFV 592
           E+    EA+EDDGYVV+YVHDE +GES F+VMDAKSP L+I+A V+LPRRVPYGFHGLFV
Sbjct: 541 EN---PEADEDDGYVVTYVHDEKAGESSFLVMDAKSPRLDIVADVRLPRRVPYGFHGLFV 597

BLAST of Cucsa.180180 vs. TrEMBL
Match: A0SE35_9ROSI (Carotenoid cleavage dioxygenase 4a OS=Citrus clementina GN=CCD4a PE=2 SV=1)

HSP 1 Score: 873.6 bits (2256), Expect = 1.3e-250
Identity = 427/610 (70.00%), Postives = 504/610 (82.62%), Query Frame = 1

Query: 1   MDSISSPFLSGRNLILSPPISSSL--------PPISTPIYSVLTEQ----NVKKNTPPPD 60
           MDS SS F S    +L P + +SL         P S  + SV  E+    N    TP   
Sbjct: 1   MDSFSSSFFSS---VLPPKLLTSLVIIPHKSTKPTSFSVSSVRIEEKPAVNQDPKTPTTT 60

Query: 61  ADSPSPPLPRPSPPSPPMPRVSST----RRVQPSLPARFFNAFDDLINNFINPPVSPSVD 120
             +P+  + +P P   P PR  S+    +R +P++P    NA DD+INNFI+PP+  SVD
Sbjct: 61  TSTPTRTVSQPQPK--PQPRTQSSYLPKKRAEPTIPTIILNACDDIINNFIDPPLKYSVD 120

Query: 121 PRYILADNFAPVDELPPTECEVIYGSLPSSLNGAYIRNGPNPQYLPRGPYHLFDGDGMLH 180
           PR++L+DNFAPVDELPPTECEV+ GSLPS L+GAYIRNGPNPQYLPRGPYHLFDGDGMLH
Sbjct: 121 PRHVLSDNFAPVDELPPTECEVVQGSLPSCLDGAYIRNGPNPQYLPRGPYHLFDGDGMLH 180

Query: 181 SLRISDGRAVLCSRYVKTYKYTLERDAGHPVFPNVFSGFNGLTASAARGAVAVGRILTGQ 240
           S++IS GRA LCSRYV+TYKYT+E +AG P+ PNVFSGFNGLTASAARGA++  R+L GQ
Sbjct: 181 SIKISRGRATLCSRYVRTYKYTIENEAGSPILPNVFSGFNGLTASAARGALSAARLLAGQ 240

Query: 241 YNPANGIGLANTSLAFFGDRLYALGESDLPYPIRLTPNGDIETLARHDFDGKLTLSMTAH 300
           +NP NGIGLANT+LAFFG+RLYALGESDLPY IRLTPNGDIET+ RHDFDGKL +SMTAH
Sbjct: 241 FNPVNGIGLANTNLAFFGNRLYALGESDLPYAIRLTPNGDIETIGRHDFDGKLAMSMTAH 300

Query: 301 PKVDSDTGEAFAFRYGPLPPFLTYFRFDKNGAKHSDVPILSMNRPSFLHDFAITKKYAVF 360
           PK+DSDTGEAFAFRYGP+PPFLTYFRFD NG K  +VPI SM RPSFLHDFAITKKYAVF
Sbjct: 301 PKLDSDTGEAFAFRYGPVPPFLTYFRFDANGKKQPNVPIFSMTRPSFLHDFAITKKYAVF 360

Query: 361 TDIQIGINPTQMIIEGGSPVGSDPSKISRVGLIPRYANDESKMKWFDVPGLNLIHAINAW 420
            DIQIG+NP +MI  GGSPVG+DP+K+ R+G+IPRYA DES+M+WF+VPG N+IHAINAW
Sbjct: 361 VDIQIGMNPMEMIFGGGSPVGADPAKVCRIGIIPRYATDESQMRWFEVPGFNIIHAINAW 420

Query: 421 DEDD--AVVIVAPNILSVEHALERMDLVHALVEKIRIDLKTGIVTRTPLSTRNLDFGVIH 480
           DE+D  AVV+VAPNILSVEH L+R DLVHALVEK+RIDL+TGIVTR P+S RNLDFGVI+
Sbjct: 421 DEEDGNAVVMVAPNILSVEHTLDRTDLVHALVEKVRIDLRTGIVTRRPMSARNLDFGVIN 480

Query: 481 PSYVGKKHRFVYAGVGDPMPKISGVVKLEISQ-EERRDCIVACRIFGPGCYGGEPFFVPR 540
           P+Y+ KK R+VYA VGDPMPKISGVVKL++S+ +ERRDCIVA RI+GPGCYGGEPFFV R
Sbjct: 481 PAYMAKKSRYVYAAVGDPMPKISGVVKLDVSKGDERRDCIVATRIYGPGCYGGEPFFVAR 540

Query: 541 ERESSDETEAEEDDGYVVSYVHDENSGESRFIVMDAKSPELEIIAAVKLPRRVPYGFHGL 592
           + E+    E EEDDGYVVSYVHDEN+GES+F+VMDAKSP L+I+AAVKLPRRVPYGFHGL
Sbjct: 541 DPEN---PETEEDDGYVVSYVHDENTGESKFLVMDAKSPRLDIVAAVKLPRRVPYGFHGL 600

BLAST of Cucsa.180180 vs. TrEMBL
Match: B9IQS5_POPTR (Uncharacterized protein OS=Populus trichocarpa GN=POPTR_0019s12320g PE=4 SV=2)

HSP 1 Score: 870.5 bits (2248), Expect = 1.1e-249
Identity = 429/615 (69.76%), Postives = 501/615 (81.46%), Query Frame = 1

Query: 1   MDSISSPFLSG--RNLILSPPISSSLPP---ISTPIY-----------SVLTEQNVKKNT 60
           MD+ SS FLS    + +L+  ++ ++P     +TP +           SV  E+  + +T
Sbjct: 1   MDASSSSFLSAIQTSKLLTGTMAMTIPKSAVTTTPSFLSRHLPSLNVSSVRIEEKPQNST 60

Query: 61  PPPDADSPSPP------LPRPSPPSPPMPR--VSSTRRVQPSLPARFFNAFDDLINNFIN 120
             P     S P      LP  + P+P   +   +  R V+P+ P   FN  + +INNFI+
Sbjct: 61  TRPTTSRTSRPASSTTTLPAATKPTPSTRKSPANDRRVVEPNQPTMMFNVLEGVINNFID 120

Query: 121 PPVSPSVDPRYILADNFAPVDELPPTECEVIYGSLPSSLNGAYIRNGPNPQYLPRGPYHL 180
           PP+  SVDPRY+L+DNFAPVDELPPTECEVI+GSLPS L+GAYIRNGPNPQYLPRGPYHL
Sbjct: 121 PPLRQSVDPRYVLSDNFAPVDELPPTECEVIHGSLPSCLDGAYIRNGPNPQYLPRGPYHL 180

Query: 181 FDGDGMLHSLRISDGRAVLCSRYVKTYKYTLERDAGHPVFPNVFSGFNGLTASAARGAVA 240
           FDGDGMLHS+RIS G+A LCSRYVKTYKYT+ERDAG P+ PNVFSGFNGL ASAARGA++
Sbjct: 181 FDGDGMLHSIRISQGKATLCSRYVKTYKYTMERDAGAPLLPNVFSGFNGLAASAARGALS 240

Query: 241 VGRILTGQYNPANGIGLANTSLAFFGDRLYALGESDLPYPIRLTPNGDIETLARHDFDGK 300
             RIL GQ+NPANGIGLANTSLA+FG+RLYALGESDLPY +RLT NGDIETL RHDFD K
Sbjct: 241 AARILAGQFNPANGIGLANTSLAYFGNRLYALGESDLPYAVRLTSNGDIETLGRHDFDRK 300

Query: 301 LTLSMTAHPKVDSDTGEAFAFRYGPLPPFLTYFRFDKNGAKHSDVPILSMNRPSFLHDFA 360
           L +SMTAHPKVD +TGEAFAFRYGP+PPFLTYF FD NG K  DVPI SM RPSFLHDF 
Sbjct: 301 LLMSMTAHPKVDLETGEAFAFRYGPVPPFLTYFHFDGNGNKQPDVPIFSMTRPSFLHDFG 360

Query: 361 ITKKYAVFTDIQIGINPTQMIIEGGSPVGSDPSKISRVGLIPRYANDESKMKWFDVPGLN 420
           I+ KYA+F DIQIG+NP +MI  GGSPVGSDP+K+SR+G+IPRYA DES+MKWFDVPG N
Sbjct: 361 ISSKYAIFADIQIGMNPMEMIFGGGSPVGSDPAKVSRLGIIPRYATDESEMKWFDVPGFN 420

Query: 421 LIHAINAWDEDDAVVIVAPNILSVEHALERMDLVHALVEKIRIDLKTGIVTRTPLSTRNL 480
           +IHAINAWDE+DAVVI+APNILSVEH LERMDL+HALVEK+RIDLKTGIVTR P+S RNL
Sbjct: 421 IIHAINAWDEEDAVVILAPNILSVEHTLERMDLIHALVEKVRIDLKTGIVTRNPVSARNL 480

Query: 481 DFGVIHPSYVGKKHRFVYAGVGDPMPKISGVVKLEISQEERRDCIVACRIFGPGCYGGEP 540
           DFGVI+P+Y+GKK+RFVYA +GDPMPKISGVVKL++S+ ER++C VA RIFGP CYGGEP
Sbjct: 481 DFGVINPAYLGKKNRFVYAAIGDPMPKISGVVKLDVSKGERQECTVASRIFGPRCYGGEP 540

Query: 541 FFVPRERESSDETEAEEDDGYVVSYVHDENSGESRFIVMDAKSPELEIIAAVKLPRRVPY 592
           FFV RE E+    EAEEDDGYVVSYVHDE +GES+F+VMDAKSP L+I+AAV+LPRRVPY
Sbjct: 541 FFVAREPEN---PEAEEDDGYVVSYVHDETAGESKFLVMDAKSPGLDIVAAVRLPRRVPY 600

BLAST of Cucsa.180180 vs. TAIR10
Match: AT4G19170.1 (AT4G19170.1 nine-cis-epoxycarotenoid dioxygenase 4)

HSP 1 Score: 783.5 bits (2022), Expect = 9.3e-227
Identity = 388/600 (64.67%), Postives = 475/600 (79.17%), Query Frame = 1

Query: 1   MDSISSP-FLSGR-NLILSPPISSSLPPISTPIYSVLTEQNVKKNTPPPDADSPSPPLPR 60
           MDS+SS  FLS   +L  S     S  P    I S + E+      P  + D  + P   
Sbjct: 1   MDSVSSSSFLSSTFSLHHSLLRRRSSSPTLLRINSAVVEERSPITNPSDNNDRRNKPKTL 60

Query: 61  PSPPSPPMPRVSSTRRVQP--SLPARFFNAFDDLINNFINPPVSPSVDPRYILADNFAPV 120
            +  +  +  VSS  +++P  +L    F   +D+IN FI+PP  PSVDP+++L+DNFAPV
Sbjct: 61  HNRTNHTL--VSSPPKLRPEMTLATALFTTVEDVINTFIDPPSRPSVDPKHVLSDNFAPV 120

Query: 121 -DELPPTECEVIYGSLPSSLNGAYIRNGPNPQYLPRGPYHLFDGDGMLHSLRISDGRAVL 180
            DELPPT+CE+I+G+LP SLNGAYIRNGPNPQ+LPRGPYHLFDGDGMLH+++I +G+A L
Sbjct: 121 LDELPPTDCEIIHGTLPLSLNGAYIRNGPNPQFLPRGPYHLFDGDGMLHAIKIHNGKATL 180

Query: 181 CSRYVKTYKYTLERDAGHPVFPNVFSGFNGLTASAARGAVAVGRILTGQYNPANGIGLAN 240
           CSRYVKTYKY +E+  G PV PNVFSGFNG+TAS ARGA+   R+LTGQYNP NGIGLAN
Sbjct: 181 CSRYVKTYKYNVEKQTGAPVMPNVFSGFNGVTASVARGALTAARVLTGQYNPVNGIGLAN 240

Query: 241 TSLAFFGDRLYALGESDLPYPIRLTPNGDIETLARHDFDGKLTLSMTAHPKVDSDTGEAF 300
           TSLAFF +RL+ALGESDLPY +RLT +GDIET+ R+DFDGKL +SMTAHPK D  TGE F
Sbjct: 241 TSLAFFSNRLFALGESDLPYAVRLTESGDIETIGRYDFDGKLAMSMTAHPKTDPITGETF 300

Query: 301 AFRYGPLPPFLTYFRFDKNGAKHSDVPILSMNRPSFLHDFAITKKYAVFTDIQIG--INP 360
           AFRYGP+PPFLTYFRFD  G K  DVPI SM  PSFLHDFAITK++A+F +IQ+G  +N 
Sbjct: 301 AFRYGPVPPFLTYFRFDSAGKKQRDVPIFSMTSPSFLHDFAITKRHAIFAEIQLGMRMNM 360

Query: 361 TQMIIEGGSPVGSDPSKISRVGLIPRYANDESKMKWFDVPGLNLIHAINAWDEDD--AVV 420
             +++EGGSPVG+D  K  R+G+IP+YA DES+MKWF+VPG N+IHAINAWDEDD  +VV
Sbjct: 361 LDLVLEGGSPVGTDNGKTPRLGVIPKYAGDESEMKWFEVPGFNIIHAINAWDEDDGNSVV 420

Query: 421 IVAPNILSVEHALERMDLVHALVEKIRIDLKTGIVTRTPLSTRNLDFGVIHPSYVGKKHR 480
           ++APNI+S+EH LERMDLVHALVEK++IDL TGIV R P+S RNLDF VI+P+++G+  R
Sbjct: 421 LIAPNIMSIEHTLERMDLVHALVEKVKIDLVTGIVRRHPISARNLDFAVINPAFLGRCSR 480

Query: 481 FVYAGVGDPMPKISGVVKLEISQEERRDCIVACRIFGPGCYGGEPFFVPRERESSDETEA 540
           +VYA +GDPMPKISGVVKL++S+ +R DC VA R++G GCYGGEPFFV R+       EA
Sbjct: 481 YVYAAIGDPMPKISGVVKLDVSKGDRDDCTVARRMYGSGCYGGEPFFVARD---PGNPEA 540

Query: 541 EEDDGYVVSYVHDENSGESRFIVMDAKSPELEIIAAVKLPRRVPYGFHGLFVKESDLNKL 592
           EEDDGYVV+YVHDE +GES+F+VMDAKSPELEI+AAV+LPRRVPYGFHGLFVKESDLNKL
Sbjct: 541 EEDDGYVVTYVHDEVTGESKFLVMDAKSPELEIVAAVRLPRRVPYGFHGLFVKESDLNKL 595

BLAST of Cucsa.180180 vs. TAIR10
Match: AT3G14440.1 (AT3G14440.1 nine-cis-epoxycarotenoid dioxygenase 3)

HSP 1 Score: 384.4 bits (986), Expect = 1.3e-106
Identity = 228/611 (37.32%), Postives = 339/611 (55.48%), Query Frame = 1

Query: 3   SISSPFLSGRNLILSPPISSSLPP-----ISTPIYSVLTEQ-NVKKNTPPPDADSPSPPL 62
           ++S  +L G +    PP+SSS         S P+ S +T + NV      P    P+   
Sbjct: 9   AVSGRWLGGNHT--QPPLSSSQSSDLSYCSSLPMASRVTRKLNVSSALHTP----PALHF 68

Query: 63  PRPSPPSPPM---PRVSSTRRVQPSLPARFFNAFDDLINNFINP-----PVSPSVDPRYI 122
           P+ S  SP +   P+   +   Q +L  R   A  D    F+       P+  + DP   
Sbjct: 69  PKQSSNSPAIVVKPKAKESNTKQMNLFQRAAAAALDAAEGFLVSHEKLHPLPKTADPSVQ 128

Query: 123 LADNFAPVDELPPTECEVIYGSLPSSLNGAYIRNGPNPQYLPRGPYHLFDGDGMLHSLRI 182
           +A NFAPV+E P      + G LP S+ G Y+RNG NP + P   +H FDGDGM+H+++ 
Sbjct: 129 IAGNFAPVNEQPVRRNLPVVGKLPDSIKGVYVRNGANPLHEPVTGHHFFDGDGMVHAVKF 188

Query: 183 SDGRAVLCSRYVKTYKYTLERDAGHPVFPNVFSGFNGLTASAARGAVAVGRILTGQYNPA 242
             G A    R+ +T ++  ER  G PVFP      +G T   AR  +   R   G  +PA
Sbjct: 189 EHGSASYACRFTQTNRFVQERQLGRPVFPKAIGELHGHT-GIARLMLFYARAAAGIVDPA 248

Query: 243 NGIGLANTSLAFFGDRLYALGESDLPYPIRLTPNGDIETLARHDFDGKLTLSMTAHPKVD 302
           +G G+AN  L +F  RL A+ E DLPY +++TPNGD++T+ R DFDG+L  +M AHPKVD
Sbjct: 249 HGTGVANAGLVYFNGRLLAMSEDDLPYQVQITPNGDLKTVGRFDFDGQLESTMIAHPKVD 308

Query: 303 SDTGEAFAFRYGPL-PPFLTYFRFDKNGAKHSDVPILSMNRPSFLHDFAITKKYAVFTDI 362
            ++GE FA  Y  +  P+L YFRF  +G K  DV I  +++P+ +HDFAIT+ + V  D 
Sbjct: 309 PESGELFALSYDVVSKPYLKYFRFSPDGTKSPDVEI-QLDQPTMMHDFAITENFVVVPDQ 368

Query: 363 QIGINPTQMIIEGGSPVGSDPSKISRVGLIPRYANDESKMKWFDVPGLNLIHAINAWD-- 422
           Q+     +M I GGSPV  D +K++R G++ +YA D S +KW D P     H  NAW+  
Sbjct: 369 QVVFKLPEM-IRGGSPVVYDKNKVARFGILDKYAEDSSNIKWIDAPDCFCFHLWNAWEEP 428

Query: 423 EDDAVVIVAPNILSVEHALERMD-LVHALVEKIRIDLKTGIVTRTPLSTR-----NLDFG 482
           E D VV++   +   +      D  + +++ +IR++LKTG  TR P+ +      NL+ G
Sbjct: 429 ETDEVVVIGSCMTPPDSIFNESDENLKSVLSEIRLNLKTGESTRRPIISNEDQQVNLEAG 488

Query: 483 VIHPSYVGKKHRFVYAGVGDPMPKISGVVKLEISQEERRDCIVACRIFGPGCYGGEPFFV 542
           +++ + +G+K +F Y  + +P PK+SG  K++++  E     V   ++G   YGGEP F+
Sbjct: 489 MVNRNMLGRKTKFAYLALAEPWPKVSGFAKVDLTTGE-----VKKHLYGDNRYGGEPLFL 548

Query: 543 PRERESSDETEAEEDDGYVVSYVHDENSGESRFIVMDAKSPELEIIAAVKLPRRVPYGFH 591
           P E         EED+GY++ +VHDE + +S   +++A S  LE+ A VKLP RVPYGFH
Sbjct: 549 PGE-------GGEEDEGYILCFVHDEKTWKSELQIVNAVS--LEVEATVKLPSRVPYGFH 596

BLAST of Cucsa.180180 vs. TAIR10
Match: AT1G30100.1 (AT1G30100.1 nine-cis-epoxycarotenoid dioxygenase 5)

HSP 1 Score: 374.8 bits (961), Expect = 9.9e-104
Identity = 220/582 (37.80%), Postives = 325/582 (55.84%), Query Frame = 1

Query: 19  PISSSLPPISTPIYSVLTEQNVKKNTPPPDADSPS----PPLPRPSPPSPPMPRVSSTRR 78
           P     P  S+ +    T+   +K +    +D+P+    P  P P+P  P     S    
Sbjct: 20  PSDLDAPSPSSSVSFTNTKPRRRKLSANSVSDTPNLLNFPNYPSPNPIIPEKD-TSRWNP 79

Query: 79  VQPSLPARFFNAFDDLINNFINPPVSPSVDPRYILADNFAPVDELPPTECEVIYGSLPSS 138
           +Q +  A    A   L+    + P+  +VDPR+ ++ N+APV E        + G +P  
Sbjct: 80  LQRAASAALDFAETALLRRERSKPLPKTVDPRHQISGNYAPVPEQSVKSSLSVDGKIPDC 139

Query: 139 LNGAYIRNGPNPQYLPRGPYHLFDGDGMLHSLRISDGRAVLCSRYVKTYKYTLERDAGHP 198
           ++G Y+RNG NP + P   +HLFDGDGM+H+++I++G A    R+ +T +   E+  G P
Sbjct: 140 IDGVYLRNGANPLFEPVSGHHLFDGDGMVHAVKITNGDASYSCRFTETERLVQEKQLGSP 199

Query: 199 VFPNVFSGFNGLTASAARGAVAVGRILTGQYNPANGIGLANTSLAFFGDRLYALGESDLP 258
           +FP      +G  +  AR  +   R L G  N  NG G+AN  L +F DRL A+ E DLP
Sbjct: 200 IFPKAIGELHG-HSGIARLMLFYARGLFGLLNHKNGTGVANAGLVYFHDRLLAMSEDDLP 259

Query: 259 YPIRLTPNGDIETLARHDFDGKLTLSMTAHPKVDSDTGEAFAFRYGPL-PPFLTYFRFDK 318
           Y +R+T NGD+ET+ R DFDG+L+ +M AHPK+D  T E FA  Y  +  P+L YF+F  
Sbjct: 260 YQVRVTDNGDLETIGRFDFDGQLSSAMIAHPKIDPVTKELFALSYDVVKKPYLKYFKFSP 319

Query: 319 NGAKHSDVPILSMNRPSFLHDFAITKKYAVFTDIQIGINPTQMIIEGGSPVGSDPSKISR 378
            G K  DV I  +  P+ +HDFAIT+ + V  D Q+    + M + G SPV  D  KISR
Sbjct: 320 EGEKSPDVEI-PLASPTMMHDFAITENFVVIPDQQVVFKLSDMFL-GKSPVKYDGEKISR 379

Query: 379 VGLIPRYANDESKMKWFDVPGLNLIHAINAWD--EDDAVVIVAPNILSVEHALERMD-LV 438
            G++PR A D S+M W + P     H  NAW+  E D VV++   +   +      D  +
Sbjct: 380 FGILPRNAKDASEMVWVESPETFCFHLWNAWESPETDEVVVIGSCMTPADSIFNECDEQL 439

Query: 439 HALVEKIRIDLKTGIVTRTPL----STRNLDFGVIHPSYVGKKHRFVYAGVGDPMPKISG 498
           ++++ +IR++LKTG  TR  +       NL+ G+++ + +G+K R+ Y  + +P PK+SG
Sbjct: 440 NSVLSEIRLNLKTGKSTRRTIIPGSVQMNLEAGMVNRNLLGRKTRYAYLAIAEPWPKVSG 499

Query: 499 VVKLEISQEERRDCIVACRIFGPGCYGGEPFFVPRERESSDETEAEEDDGYVVSYVHDEN 558
             K+++S  E     V    +G   YGGEPFF+PR  ES       EDDGY++S+VHDE 
Sbjct: 500 FAKVDLSTGE-----VKNHFYGGKKYGGEPFFLPRGLESDG-----EDDGYIMSFVHDEE 559

Query: 559 SGESRFIVMDAKSPELEIIAAVKLPRRVPYGFHGLFVKESDL 589
           S ES   +++A + ELE  A VKLP RVPYGFHG FV  +D+
Sbjct: 560 SWESELHIVNAVTLELE--ATVKLPSRVPYGFHGTFVNSADM 585

BLAST of Cucsa.180180 vs. TAIR10
Match: AT3G63520.1 (AT3G63520.1 carotenoid cleavage dioxygenase 1)

HSP 1 Score: 368.2 bits (944), Expect = 9.3e-102
Identity = 212/538 (39.41%), Postives = 306/538 (56.88%), Query Frame = 1

Query: 70  SSTRRVQPSLPARFFNAFDDLINNFINPPVSPSVDPRYILADNFAPV-DELPPTECEVIY 129
           SS   V P     F +   DL+   +   +  +  P + L+ NFAP+ DE PP +   ++
Sbjct: 9   SSIISVHPRPSKGFSSKLLDLLERLVVKLMHDASLPLHYLSGNFAPIRDETPPVKDLPVH 68

Query: 130 GSLPSSLNGAYIRNGPNPQYLPRGPYHLFDGDGMLHSLRISDGRAVLCSRYVKTYKYTLE 189
           G LP  LNG ++R GPNP++     YH FDGDGM+H +RI DG+A   SRYVKT +   E
Sbjct: 69  GFLPECLNGEFVRVGPNPKFDAVAGYHWFDGDGMIHGVRIKDGKATYVSRYVKTSRLKQE 128

Query: 190 RDAGHPVFPNV--FSGFNGLTASAARGAVAVGRILTGQYNPANGIGLANTSLAFFGDRLY 249
              G   F  +    GF GL     +      +IL   Y    G G ANT+L +   +L 
Sbjct: 129 EFFGAAKFMKIGDLKGFFGLLMVNVQQLRTKLKILDNTY----GNGTANTALVYHHGKLL 188

Query: 250 ALGESDLPYPIRLTPNGDIETLARHDFDGKLTLSMTAHPKVDSDTGEAFAFRYGPLPPFL 309
           AL E+D PY I++  +GD++TL   D+D +LT S TAHPKVD  TGE F F Y   PP+L
Sbjct: 189 ALQEADKPYVIKVLEDGDLQTLGIIDYDKRLTHSFTAHPKVDPVTGEMFTFGYSHTPPYL 248

Query: 310 TYFRFDKNGAKHSDVPILSMNRPSFLHDFAITKKYAVFTDIQIGINPTQMIIEGGSPVGS 369
           TY    K+G  H  VPI +++ P  +HDFAIT+ YA+F D+ +   P +M+ E       
Sbjct: 249 TYRVISKDGIMHDPVPI-TISEPIMMHDFAITETYAIFMDLPMHFRPKEMVKEKKMIYSF 308

Query: 370 DPSKISRVGLIPRYANDESKMKWFDVPGLNLIHAINAWDEDDAVVIVA-----PNILSVE 429
           DP+K +R G++PRYA DE  ++WF++P   + H  NAW+E+D VV++      P++  V 
Sbjct: 309 DPTKKARFGVLPRYAKDELMIRWFELPNCFIFHNANAWEEEDEVVLITCRLENPDLDMVS 368

Query: 430 HAL-ERMDLVHALVEKIRIDLKTGIVTRTPLSTRNLDFGVIHPSYVGKKHRFVYAGVGDP 489
             + E+++     + ++R ++KTG  ++  LS   +DF  I+  Y GKK R+VY  + D 
Sbjct: 369 GKVKEKLENFGNELYEMRFNMKTGSASQKKLSASAVDFPRINECYTGKKQRYVYGTILDS 428

Query: 490 MPKISGVVKLEISQEE---RRDCIVACRI-----FGPGCYGGEPFFVPRERESSDETEAE 549
           + K++G++K ++  E    +R   V   I      G G YG E  +VPRE        AE
Sbjct: 429 IAKVTGIIKFDLHAEAETGKRMLEVGGNIKGIYDLGEGRYGSEAIYVPRE-------TAE 488

Query: 550 EDDGYVVSYVHDENSGESRFIVMDAKSPELEIIAAVKLPRRVPYGFHGLFVKESDLNK 591
           EDDGY++ +VHDEN+G+S   V+DAK+   E +A V+LP RVPYGFH LFV E  L +
Sbjct: 489 EDDGYLIFFVHDENTGKSCVTVIDAKTMSAEPVAVVELPHRVPYGFHALFVTEEQLQE 534

BLAST of Cucsa.180180 vs. TAIR10
Match: AT1G78390.1 (AT1G78390.1 nine-cis-epoxycarotenoid dioxygenase 9)

HSP 1 Score: 350.9 bits (899), Expect = 1.5e-96
Identity = 195/503 (38.77%), Postives = 291/503 (57.85%), Query Frame = 1

Query: 98  PVSPSVDPRYILADNFAPVDELPPTECEVIYGSLPSSLNGAYIRNGPNPQYLPRGPYHLF 157
           P   + DP   +A NF PV E P      + G++P  + G Y+RNG NP + P   +HLF
Sbjct: 172 PHPKTADPAVQIAGNFFPVPEKPVVHNLPVTGTVPECIQGVYVRNGANPLHKPVSGHHLF 231

Query: 158 DGDGMLHSLRISDGRAVLCSRYVKTYKYTLERDAGHPVFPNVFSGFNGLTASAARGAVAV 217
           DGDGM+H++R  +G      R+ +T +   ER+ G PVFP      +G     A+  +  
Sbjct: 232 DGDGMVHAVRFDNGSVSYACRFTETNRLVQERECGRPVFPKAIGELHG-HLGIAKLMLFN 291

Query: 218 GRILTGQYNPANGIGLANTSLAFFGDRLYALGESDLPYPIRLTPNGDIETLARHDFDGKL 277
            R L G  +P  G+G+AN  L +F   L A+ E DLPY +++T  GD+ET  R+DFDG+L
Sbjct: 292 TRGLFGLVDPTGGLGVANAGLVYFNGHLLAMSEDDLPYHVKVTQTGDLETSGRYDFDGQL 351

Query: 278 TLSMTAHPKVDSDTGEAFAFRYGPL-PPFLTYFRFDKNGAKHSDVPILSMNRPSFLHDFA 337
             +M AHPK+D +T E FA  Y  +  P+L YFRF  +G K  DV I  +++P+ +HDFA
Sbjct: 352 KSTMIAHPKIDPETRELFALSYDVVSKPYLKYFRFTSDGEKSPDVEI-PLDQPTMIHDFA 411

Query: 338 ITKKYAVFTDIQIGINPTQMIIEGGSPVGSDPSKISRVGLIPRYANDESKMKWFDVPGLN 397
           IT+ + V  D Q+     +M I GGSPV  D  K SR G++ + A D S ++W +VP   
Sbjct: 412 ITENFVVIPDQQVVFRLPEM-IRGGSPVVYDEKKKSRFGILNKNAKDASSIQWIEVPDCF 471

Query: 398 LIHAINAWDE---DDAVVI----VAPNILSVEHALERMDLVHALVEKIRIDLKTGIVTRT 457
             H  N+W+E   D+ VVI      P+ +  EH     + + +++ +IR++LKTG  TR 
Sbjct: 472 CFHLWNSWEEPETDEVVVIGSCMTPPDSIFNEHD----ETLQSVLSEIRLNLKTGESTRR 531

Query: 458 PLSTR--NLDFGVIHPSYVGKKHRFVYAGVGDPMPKISGVVKLEISQEERRDCIVACRIF 517
           P+ +   NL+ G+++ + +G+K R+ Y  + +P PK+SG  K+++S  E R       I+
Sbjct: 532 PVISEQVNLEAGMVNRNLLGRKTRYAYLALTEPWPKVSGFAKVDLSTGEIRK-----YIY 591

Query: 518 GPGCYGGEPFFVPRERESSDETEAEEDDGYVVSYVHDENSGESRFIVMDAKSPELEIIAA 577
           G G YGGEP F+P         + EED GY++ +VHDE   +S   +++A + +LE  A 
Sbjct: 592 GEGKYGGEPLFLP-------SGDGEEDGGYIMVFVHDEEKVKSELQLINAVNMKLE--AT 651

Query: 578 VKLPRRVPYGFHGLFVKESDLNK 591
           V LP RVPYGFHG F+ + DL+K
Sbjct: 652 VTLPSRVPYGFHGTFISKEDLSK 653

BLAST of Cucsa.180180 vs. NCBI nr
Match: gi|449460074|ref|XP_004147771.1| (PREDICTED: probable carotenoid cleavage dioxygenase 4, chloroplastic [Cucumis sativus])

HSP 1 Score: 1205.3 bits (3117), Expect = 0.0e+00
Identity = 591/591 (100.00%), Postives = 591/591 (100.00%), Query Frame = 1

Query: 1   MDSISSPFLSGRNLILSPPISSSLPPISTPIYSVLTEQNVKKNTPPPDADSPSPPLPRPS 60
           MDSISSPFLSGRNLILSPPISSSLPPISTPIYSVLTEQNVKKNTPPPDADSPSPPLPRPS
Sbjct: 1   MDSISSPFLSGRNLILSPPISSSLPPISTPIYSVLTEQNVKKNTPPPDADSPSPPLPRPS 60

Query: 61  PPSPPMPRVSSTRRVQPSLPARFFNAFDDLINNFINPPVSPSVDPRYILADNFAPVDELP 120
           PPSPPMPRVSSTRRVQPSLPARFFNAFDDLINNFINPPVSPSVDPRYILADNFAPVDELP
Sbjct: 61  PPSPPMPRVSSTRRVQPSLPARFFNAFDDLINNFINPPVSPSVDPRYILADNFAPVDELP 120

Query: 121 PTECEVIYGSLPSSLNGAYIRNGPNPQYLPRGPYHLFDGDGMLHSLRISDGRAVLCSRYV 180
           PTECEVIYGSLPSSLNGAYIRNGPNPQYLPRGPYHLFDGDGMLHSLRISDGRAVLCSRYV
Sbjct: 121 PTECEVIYGSLPSSLNGAYIRNGPNPQYLPRGPYHLFDGDGMLHSLRISDGRAVLCSRYV 180

Query: 181 KTYKYTLERDAGHPVFPNVFSGFNGLTASAARGAVAVGRILTGQYNPANGIGLANTSLAF 240
           KTYKYTLERDAGHPVFPNVFSGFNGLTASAARGAVAVGRILTGQYNPANGIGLANTSLAF
Sbjct: 181 KTYKYTLERDAGHPVFPNVFSGFNGLTASAARGAVAVGRILTGQYNPANGIGLANTSLAF 240

Query: 241 FGDRLYALGESDLPYPIRLTPNGDIETLARHDFDGKLTLSMTAHPKVDSDTGEAFAFRYG 300
           FGDRLYALGESDLPYPIRLTPNGDIETLARHDFDGKLTLSMTAHPKVDSDTGEAFAFRYG
Sbjct: 241 FGDRLYALGESDLPYPIRLTPNGDIETLARHDFDGKLTLSMTAHPKVDSDTGEAFAFRYG 300

Query: 301 PLPPFLTYFRFDKNGAKHSDVPILSMNRPSFLHDFAITKKYAVFTDIQIGINPTQMIIEG 360
           PLPPFLTYFRFDKNGAKHSDVPILSMNRPSFLHDFAITKKYAVFTDIQIGINPTQMIIEG
Sbjct: 301 PLPPFLTYFRFDKNGAKHSDVPILSMNRPSFLHDFAITKKYAVFTDIQIGINPTQMIIEG 360

Query: 361 GSPVGSDPSKISRVGLIPRYANDESKMKWFDVPGLNLIHAINAWDEDDAVVIVAPNILSV 420
           GSPVGSDPSKISRVGLIPRYANDESKMKWFDVPGLNLIHAINAWDEDDAVVIVAPNILSV
Sbjct: 361 GSPVGSDPSKISRVGLIPRYANDESKMKWFDVPGLNLIHAINAWDEDDAVVIVAPNILSV 420

Query: 421 EHALERMDLVHALVEKIRIDLKTGIVTRTPLSTRNLDFGVIHPSYVGKKHRFVYAGVGDP 480
           EHALERMDLVHALVEKIRIDLKTGIVTRTPLSTRNLDFGVIHPSYVGKKHRFVYAGVGDP
Sbjct: 421 EHALERMDLVHALVEKIRIDLKTGIVTRTPLSTRNLDFGVIHPSYVGKKHRFVYAGVGDP 480

Query: 481 MPKISGVVKLEISQEERRDCIVACRIFGPGCYGGEPFFVPRERESSDETEAEEDDGYVVS 540
           MPKISGVVKLEISQEERRDCIVACRIFGPGCYGGEPFFVPRERESSDETEAEEDDGYVVS
Sbjct: 481 MPKISGVVKLEISQEERRDCIVACRIFGPGCYGGEPFFVPRERESSDETEAEEDDGYVVS 540

Query: 541 YVHDENSGESRFIVMDAKSPELEIIAAVKLPRRVPYGFHGLFVKESDLNKL 592
           YVHDENSGESRFIVMDAKSPELEIIAAVKLPRRVPYGFHGLFVKESDLNKL
Sbjct: 541 YVHDENSGESRFIVMDAKSPELEIIAAVKLPRRVPYGFHGLFVKESDLNKL 591

BLAST of Cucsa.180180 vs. NCBI nr
Match: gi|659101954|ref|XP_008451876.1| (PREDICTED: probable carotenoid cleavage dioxygenase 4, chloroplastic [Cucumis melo])

HSP 1 Score: 1133.2 bits (2930), Expect = 0.0e+00
Identity = 556/592 (93.92%), Postives = 570/592 (96.28%), Query Frame = 1

Query: 1   MDSISSPFLSGRNLILSPPISSSLPPISTPIYSVLTEQNVKKNTPPPDADSPSPP-LPRP 60
           MDSISSPFLS  NLILSPPIS+S PPISTPIYSVLTEQ  KKNTPPPDADSPSP  LPR 
Sbjct: 1   MDSISSPFLSRGNLILSPPISTSRPPISTPIYSVLTEQTAKKNTPPPDADSPSPSSLPRR 60

Query: 61  SPPSPPMPRVSSTRRVQPSLPARFFNAFDDLINNFINPPVSPSVDPRYILADNFAPVDEL 120
           SPPSP M RVSSTRRV+PSLPAR FNAFDDLINNFINPP+SPSVDPRYILADNFAPVDEL
Sbjct: 61  SPPSPAMARVSSTRRVEPSLPARLFNAFDDLINNFINPPISPSVDPRYILADNFAPVDEL 120

Query: 121 PPTECEVIYGSLPSSLNGAYIRNGPNPQYLPRGPYHLFDGDGMLHSLRISDGRAVLCSRY 180
           PPTECE+IYGSLPSSLNGAYIRNGPNPQYLPRGPYHLFDGDGMLHSLRIS+GRAVLCSRY
Sbjct: 121 PPTECEIIYGSLPSSLNGAYIRNGPNPQYLPRGPYHLFDGDGMLHSLRISNGRAVLCSRY 180

Query: 181 VKTYKYTLERDAGHPVFPNVFSGFNGLTASAARGAVAVGRILTGQYNPANGIGLANTSLA 240
           VKTYKYTLERDAGHPV PNVFSGFNGLTASAARGAV+ GRILTGQYNPANGIGLANTSLA
Sbjct: 181 VKTYKYTLERDAGHPVLPNVFSGFNGLTASAARGAVSFGRILTGQYNPANGIGLANTSLA 240

Query: 241 FFGDRLYALGESDLPYPIRLTPNGDIETLARHDFDGKLTLSMTAHPKVDSDTGEAFAFRY 300
           FFGDRLYALGESDLPY IRL PNGDIETL+RHDFDGKLT SMTAHPK+DSDTGEAFAFRY
Sbjct: 241 FFGDRLYALGESDLPYLIRLAPNGDIETLSRHDFDGKLTYSMTAHPKIDSDTGEAFAFRY 300

Query: 301 GPLPPFLTYFRFDKNGAKHSDVPILSMNRPSFLHDFAITKKYAVFTDIQIGINPTQMIIE 360
           GPLPPFLTYFRFDKNGAK SDVPILSMNRPSFLHDFAITKKYAVF DIQIGINPTQMIIE
Sbjct: 301 GPLPPFLTYFRFDKNGAKQSDVPILSMNRPSFLHDFAITKKYAVFADIQIGINPTQMIIE 360

Query: 361 GGSPVGSDPSKISRVGLIPRYANDESKMKWFDVPGLNLIHAINAWDEDDAVVIVAPNILS 420
           GGSPVGSDPSKISRVGLIPRYANDESKMKWFDVPGLNL+HAINAWDEDDAVVIVAPNILS
Sbjct: 361 GGSPVGSDPSKISRVGLIPRYANDESKMKWFDVPGLNLVHAINAWDEDDAVVIVAPNILS 420

Query: 421 VEHALERMDLVHALVEKIRIDLKTGIVTRTPLSTRNLDFGVIHPSYVGKKHRFVYAGVGD 480
           VEHALERM+LVH LVEK+RIDLKTGIVTRTPLSTRNLDFGVIHPSYVGKK+RFVYA +GD
Sbjct: 421 VEHALERMNLVHGLVEKVRIDLKTGIVTRTPLSTRNLDFGVIHPSYVGKKNRFVYACIGD 480

Query: 481 PMPKISGVVKLEISQEERRDCIVACRIFGPGCYGGEPFFVPRERESSDETEAEEDDGYVV 540
           PMPKISGV KL+ISQEERRDCIVACRIFGPGCYGGEPFFVPRERESSDET AEEDDGYVV
Sbjct: 481 PMPKISGVAKLDISQEERRDCIVACRIFGPGCYGGEPFFVPRERESSDETAAEEDDGYVV 540

Query: 541 SYVHDENSGESRFIVMDAKSPELEIIAAVKLPRRVPYGFHGLFVKESDLNKL 592
           SYVHDENSGESRFIVMDAKSPELEIIAAVKLPRRVPYGFHGLFVKESDLNKL
Sbjct: 541 SYVHDENSGESRFIVMDAKSPELEIIAAVKLPRRVPYGFHGLFVKESDLNKL 592

BLAST of Cucsa.180180 vs. NCBI nr
Match: gi|408794953|gb|AFU91490.1| (carotenoid cleavage dioxygenase 4 [Momordica charantia])

HSP 1 Score: 991.9 bits (2563), Expect = 4.9e-286
Identity = 496/597 (83.08%), Postives = 532/597 (89.11%), Query Frame = 1

Query: 1   MDSISSPFLSGRNLILSPPISSSLPPISTPIYSVLTEQN---VKKNTP-PPDADSPSPPL 60
           MD+ISSPFLSG NL+LSP IS S P I+T I SVLTE+    VKK  P P DADS SP  
Sbjct: 1   MDAISSPFLSGGNLLLSPAISISRPSIATVISSVLTEETPKTVKKTGPRPSDADS-SPLR 60

Query: 61  PRPSPPS--PPMPRVSSTRRVQPSLPARFFNAFDDLINNFINPPVSPSVDPRYILADNFA 120
             P PP+  P M + SSTRRV+PSLPAR FNAFDDLINNFINPPV+PSVDPRY+LADNFA
Sbjct: 61  ATPPPPAKVPAMAKSSSTRRVEPSLPARLFNAFDDLINNFINPPVNPSVDPRYVLADNFA 120

Query: 121 PVDELPPTECEVIYGSLPSSLNGAYIRNGPNPQYLPRGPYHLFDGDGMLHSLRISDGRAV 180
           PVDELPPTECEVI GSLP SLNGAYIRNGPNPQYLPRGPYHLFDGDGMLHSLRISDGRAV
Sbjct: 121 PVDELPPTECEVIQGSLPPSLNGAYIRNGPNPQYLPRGPYHLFDGDGMLHSLRISDGRAV 180

Query: 181 LCSRYVKTYKYTLERDAGHPVFPNVFSGFNGLTASAARGAVAVGRILTGQYNPANGIGLA 240
           LCSRYVKTYKYTLERD GHPV PNVFSGFNGLTASAAR AV  GR+LTGQ++PANGIGLA
Sbjct: 181 LCSRYVKTYKYTLERDVGHPVIPNVFSGFNGLTASAARSAVTAGRMLTGQFDPANGIGLA 240

Query: 241 NTSLAFFGDRLYALGESDLPYPIRLTPNGDIETLARHDFDGKLTLSMTAHPKVDSDTGEA 300
           NTSLA+FGDRLYALGESDLPYPIRLTP GDIETL RHDFDGKL++SMTAHPKVD  TGEA
Sbjct: 241 NTSLAYFGDRLYALGESDLPYPIRLTPTGDIETLDRHDFDGKLSISMTAHPKVDPVTGEA 300

Query: 301 FAFRYGPLPPFLTYFRFDKNGAKHSDVPILSMNRPSFLHDFAITKKYAVFTDIQIGINPT 360
           FAFRYGPLPPFLT+FRFD++GAK SDVPI SM+RPSFLHDFAIT+KYAVF + QIG NP 
Sbjct: 301 FAFRYGPLPPFLTFFRFDRSGAKQSDVPIFSMSRPSFLHDFAITEKYAVFGETQIGFNPM 360

Query: 361 QMIIEGGSPVGSDPSKISRVGLIPRYANDESKMKWFDVPGLNLIHAINAWDEDDAVVIVA 420
           QMI EG SPVGS+PSK+ R G+IPRYA DESKMKWFDVPG NLIHAINAWDE DAVV+VA
Sbjct: 361 QMITEGRSPVGSNPSKVCRAGIIPRYATDESKMKWFDVPGFNLIHAINAWDEYDAVVMVA 420

Query: 421 PNILSVEHALERMDLVHALVEKIRIDLKTGIVTRTPLSTRNLDFGVIHPSYVGKKHRFVY 480
           PNILSVEH +ERMDLVHALVEK RIDLKTGIVTR  LSTRNLDFGVI+PSYVG+K+RFVY
Sbjct: 421 PNILSVEHTMERMDLVHALVEKARIDLKTGIVTRRSLSTRNLDFGVINPSYVGRKNRFVY 480

Query: 481 AGVGDPMPKISGVVKLEISQEERRDCIVACRIFGPGCYGGEPFFVPRERESSDETEAEED 540
           AGVGDPMPKISGVVKL++SQEE RDCIVA RIFGPGCYGGEPF VPRE E++ ET AEE 
Sbjct: 481 AGVGDPMPKISGVVKLDVSQEECRDCIVASRIFGPGCYGGEPFLVPREGENAGETAAEEG 540

Query: 541 DGYVVSYVHDENSGESRFIVMDAKSPELEIIAAVKLPRRVPYGFHGLFVKESDLNKL 592
           DGYVVSYVH+EN+GESRFIVMDAKSP L I+AAVKLPRRVPYGF GLFVKESDLNKL
Sbjct: 541 DGYVVSYVHNENTGESRFIVMDAKSPNLAIVAAVKLPRRVPYGFLGLFVKESDLNKL 596

BLAST of Cucsa.180180 vs. NCBI nr
Match: gi|488892479|gb|AGL08676.1| (carotenoid cleavage dioxygenase 4 [Prunus persica])

HSP 1 Score: 878.2 bits (2268), Expect = 7.8e-252
Identity = 424/608 (69.74%), Postives = 500/608 (82.24%), Query Frame = 1

Query: 1   MDSISSPFLSG---RNLILSPPISSSLPPISTPIYSVLTEQNVKKNTPPPDADSPSPPLP 60
           MD+ SS FLS    +NL LSP I+       TP +S+ + +  ++ + PP A  P+    
Sbjct: 1   MDAFSSSFLSTFPTQNLSLSPAIA-------TPKFSISSVRIEERPSSPPPASKPTST-K 60

Query: 61  RPSPPSPPMPRVSSTRR--------------VQPSLPARFFNAFDDLINNFINPPVSPSV 120
            P PP  P P +++  R                P+LPA  FNA DD+INNFI+PP+ PSV
Sbjct: 61  APQPPKTPSPPLTTKARDYNNASTFSAAKKGTDPTLPAVIFNALDDIINNFIDPPLRPSV 120

Query: 121 DPRYILADNFAPVDELPPTECEVIYGSLPSSLNGAYIRNGPNPQYLPRGPYHLFDGDGML 180
           DP+++L++NFAPVDELPPTECE+I GSLP  L+GAYIRNGPNPQYLPRGPYHLFDGDGML
Sbjct: 121 DPKHVLSNNFAPVDELPPTECEIIQGSLPPCLDGAYIRNGPNPQYLPRGPYHLFDGDGML 180

Query: 181 HSLRISDGRAVLCSRYVKTYKYTLERDAGHPVFPNVFSGFNGLTASAARGAVAVGRILTG 240
           HS+RIS GRAVLCSRYVKTYKYT+ERDAG+P+ P+VFSGFNGLTASA RGA++  R+ TG
Sbjct: 181 HSVRISKGRAVLCSRYVKTYKYTIERDAGYPILPSVFSGFNGLTASATRGALSAARVFTG 240

Query: 241 QYNPANGIGLANTSLAFFGDRLYALGESDLPYPIRLTPNGDIETLARHDFDGKLTLSMTA 300
           QYNPANGIGLANTSLAFFG++LYALGESDLPY +RLT NGDI+TL RHDFDGKL +SMTA
Sbjct: 241 QYNPANGIGLANTSLAFFGNQLYALGESDLPYSLRLTSNGDIQTLGRHDFDGKLFMSMTA 300

Query: 301 HPKVDSDTGEAFAFRYGPLPPFLTYFRFDKNGAKHSDVPILSMNRPSFLHDFAITKKYAV 360
           HPK+D +TGEAFAFRYGPLPPFLTYFRFD NG K  DVPI SM  PSFLHDFAITKKYA+
Sbjct: 301 HPKIDPETGEAFAFRYGPLPPFLTYFRFDANGTKQPDVPIFSMVTPSFLHDFAITKKYAI 360

Query: 361 FTDIQIGINPTQMIIEGGSPVGSDPSKISRVGLIPRYANDESKMKWFDVPGLNLIHAINA 420
           F DIQIG+NP  MI +G SPVG DPSK+ R+G+IPRYA DE++M+WFDVPG N+IHAINA
Sbjct: 361 FVDIQIGMNPIDMITKGASPVGLDPSKVPRIGVIPRYAKDETEMRWFDVPGFNIIHAINA 420

Query: 421 WDEDDAVVIVAPNILSVEHALERMDLVHALVEKIRIDLKTGIVTRTPLSTRNLDFGVIHP 480
           WDE+DA+V+VAPNILS EH +ERMDL+HA VEK+RIDLKTGIV+R P+STRNLDF V +P
Sbjct: 421 WDEEDAIVMVAPNILSAEHTMERMDLIHASVEKVRIDLKTGIVSRQPISTRNLDFAVFNP 480

Query: 481 SYVGKKHRFVYAGVGDPMPKISGVVKLEISQEERRDCIVACRIFGPGCYGGEPFFVPRER 540
           +YVGKK+++VYA VGDPMPKISGVVKL++S  E ++CIVA R+FGPGCYGGEPFFV RE 
Sbjct: 481 AYVGKKNKYVYAAVGDPMPKISGVVKLDVSNVEHKECIVASRMFGPGCYGGEPFFVAREP 540

Query: 541 ESSDETEAEEDDGYVVSYVHDENSGESRFIVMDAKSPELEIIAAVKLPRRVPYGFHGLFV 592
           E+    EA+EDDGYVV+YVHDE +GES F+VMDAKSP L+I+A V+LPRRVPYGFHGLFV
Sbjct: 541 EN---PEADEDDGYVVTYVHDEKAGESSFLVMDAKSPRLDIVADVRLPRRVPYGFHGLFV 597

BLAST of Cucsa.180180 vs. NCBI nr
Match: gi|645230118|ref|XP_008221785.1| (PREDICTED: probable carotenoid cleavage dioxygenase 4, chloroplastic [Prunus mume])

HSP 1 Score: 877.9 bits (2267), Expect = 1.0e-251
Identity = 426/606 (70.30%), Postives = 505/606 (83.33%), Query Frame = 1

Query: 1   MDSISSPFLSG---RNLILSPPISSSLPPISTPIYSVLTEQNVKKNTPPPDADSPSPPLP 60
           MD+ SS FLS    +N  LSP I++  P +S  I SV  E+  + ++PPP +   S   P
Sbjct: 1   MDAFSSSFLSTFPTQNFSLSPAIAT--PKLS--ISSVRIEE--RPSSPPPASKPTSTKAP 60

Query: 61  RP-SPPSPPMPRVS-----------STRRVQPSLPARFFNAFDDLINNFINPPVSPSVDP 120
           +P   PSPP+   +           + ++  P+LPA  FNA DD+INNFI+PP+ PSVDP
Sbjct: 61  QPPKTPSPPLTTKARDYNNASTFSAAKKQTDPTLPAVIFNALDDIINNFIDPPLRPSVDP 120

Query: 121 RYILADNFAPVDELPPTECEVIYGSLPSSLNGAYIRNGPNPQYLPRGPYHLFDGDGMLHS 180
           +++L++NFAPVDELPPTECE+I GSLP  L+GAYIRNGPNPQYLPRGPYHLFDGDGMLHS
Sbjct: 121 KHVLSNNFAPVDELPPTECEIIQGSLPPCLDGAYIRNGPNPQYLPRGPYHLFDGDGMLHS 180

Query: 181 LRISDGRAVLCSRYVKTYKYTLERDAGHPVFPNVFSGFNGLTASAARGAVAVGRILTGQY 240
           +RIS GRAVLCSRYVKTYKYT+ERDAG+P+ PNVFSGFNGLTASA RGA++  R+ TGQY
Sbjct: 181 VRISKGRAVLCSRYVKTYKYTIERDAGYPLLPNVFSGFNGLTASATRGALSAARVFTGQY 240

Query: 241 NPANGIGLANTSLAFFGDRLYALGESDLPYPIRLTPNGDIETLARHDFDGKLTLSMTAHP 300
           NPANGIGLANTSLAFFG++LYALGESDLPY +RLT NGDI+TL RHDFDGKL +SMTAHP
Sbjct: 241 NPANGIGLANTSLAFFGNQLYALGESDLPYSLRLTSNGDIQTLGRHDFDGKLFMSMTAHP 300

Query: 301 KVDSDTGEAFAFRYGPLPPFLTYFRFDKNGAKHSDVPILSMNRPSFLHDFAITKKYAVFT 360
           K+D +TGEAFAFRYGPLPPFLTYFRFD NG K  DVPI SM  PSFLHDFAITKKYA+F 
Sbjct: 301 KIDPETGEAFAFRYGPLPPFLTYFRFDANGTKQPDVPIFSMVTPSFLHDFAITKKYAIFV 360

Query: 361 DIQIGINPTQMIIEGGSPVGSDPSKISRVGLIPRYANDESKMKWFDVPGLNLIHAINAWD 420
           DIQIG+NP  MI +G SPVG DPSK+SR+G+IPRYA DE++M+WFDVPG N+IHAINAWD
Sbjct: 361 DIQIGMNPIDMITKGASPVGLDPSKVSRIGVIPRYAKDETEMRWFDVPGFNIIHAINAWD 420

Query: 421 EDDAVVIVAPNILSVEHALERMDLVHALVEKIRIDLKTGIVTRTPLSTRNLDFGVIHPSY 480
           E+DA+V+VAPNILS EH +ERM+L+HA VEK+RIDLKTGIV+R P+STRNLDF V +P+Y
Sbjct: 421 EEDAIVMVAPNILSAEHTMERMELIHASVEKVRIDLKTGIVSRQPISTRNLDFAVFNPAY 480

Query: 481 VGKKHRFVYAGVGDPMPKISGVVKLEISQEERRDCIVACRIFGPGCYGGEPFFVPRERES 540
           VG+K+++VYA VGDPMPKISGVVKL++S  E ++CIVA R+FGPGCYGGEPFFV RE E+
Sbjct: 481 VGRKNKYVYAAVGDPMPKISGVVKLDVSNVEHKECIVASRMFGPGCYGGEPFFVAREPEN 540

Query: 541 SDETEAEEDDGYVVSYVHDENSGESRFIVMDAKSPELEIIAAVKLPRRVPYGFHGLFVKE 592
               EA+EDDGYVV+YVHDE +GESRF+VMDAKSP  +I+A V+LPRRVPYGFHGLFVKE
Sbjct: 541 ---PEADEDDGYVVTYVHDEKAGESRFLVMDAKSPRFDIVANVRLPRRVPYGFHGLFVKE 597