Cucsa.178760 (gene) Cucumber (Gy14) v1 | Cucurbit Genomics Database (CuGenDB)

Overview


Name	Cucsa.178760
Type	gene
Organism	Cucumis sativus (Cucumber (Gy14) v1)
Description	ralf-like 9
Location	scaffold01227 : 1114950 .. 1115186 (+)

Genome Viewer

Gene Expression

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGATCATGTCAAAAAGAATCACACTTGTTTTTATTGAGATTTTCTTGATTAGCTTTAACATCCTTACGATGACAGATGCTAGAAAATTCATTGATTACCATTCTATAGTTGCAGGAGATGTTAGCCCCGGATGTAACCCAAAGCATCCTGAACTTTGTAAATTAACTCCAGCAAATCCATATGAAAGAGGTTGCAATAAAATCAATCGCTGTAGAGGAGGAAATATATATAATTGA

mRNA sequence

ATGATCATGTCAAAAAGAATCACACTTGTTTTTATTGAGATTTTCTTGATTAGCTTTAACATCCTTACGATGACAGATGCTAGAAAATTCATTGATTACCATTCTATAGTTGCAGGAGATGTTAGCCCCGGATGTAACCCAAAGCATCCTGAACTTTGTAAATTAACTCCAGCAAATCCATATGAAAGAGGTTGCAATAAAATCAATCGCTGTAGAGGAGGAAATATATATAATTGA

Coding sequence (CDS)

ATGATCATGTCAAAAAGAATCACACTTGTTTTTATTGAGATTTTCTTGATTAGCTTTAACATCCTTACGATGACAGATGCTAGAAAATTCATTGATTACCATTCTATAGTTGCAGGAGATGTTAGCCCCGGATGTAACCCAAAGCATCCTGAACTTTGTAAATTAACTCCAGCAAATCCATATGAAAGAGGTTGCAATAAAATCAATCGCTGTAGAGGAGGAAATATATATAATTGA

Protein sequence

MIMSKRITLVFIEIFLISFNILTMTDARKFIDYHSIVAGDVSPGCNPKHPELCKLTPANPYERGCNKINRCRGGNIYN*

Homology

[Close]

BLAST of Cucsa.178760 vs. Swiss-Prot
Match: RLF9_ARATH (Protein RALF-like 9 OS=Arabidopsis thaliana GN=RALFL9 PE=3 SV=1)

HSP 1 Score: 67.0 bits (162), Expect = 1.0e-10
Identity = 35/76 (46.05%), Postives = 47/76 (61.84%), Query Frame = 1


Query: 1  MIMSKRITLVFIEIFLISFNILTMT--DARKFIDYHSIVAGDVSPGCNPKHPELCKLTPA 60
          M MSK I ++ + + L+ F  L  T  +A ++I Y +I  GD +  C+  HP  CK   A
Sbjct: 1  MGMSKSIKVI-LSLALVVFLALAATKVEATRYITYPAIDRGDHAVHCDKAHPNTCKKKEA 60

Query: 61 NPYERGCNKINRCRGG 75
          NPY+RGC KINRCRGG
Sbjct: 61 NPYQRGCEKINRCRGG 75

[Close]

BLAST of Cucsa.178760 vs. Swiss-Prot
Match: RLF15_ARATH (Protein RALF-like 15 OS=Arabidopsis thaliana GN=RALFL15 PE=3 SV=1)

HSP 1 Score: 56.2 bits (134), Expect = 1.8e-07
Identity = 31/74 (41.89%), Postives = 41/74 (55.41%), Query Frame = 1


Query: 1  MIMSKRITLVFIEIFLISFNILTMT--DARKFIDYHSIVAGDVSPGCNPKHPELCKLTPA 60
          M MSK I ++ + + LI F  L  T  +A ++I Y  +  GD +  C+  HP  CK   A
Sbjct: 1  MGMSKSIKVI-VSLALILFLALAATKVEATRYISYRGMNHGDHAIHCDKAHPNTCKKQVA 60

Query: 61 NPYERGCNKINRCR 73
          NPY RGC  I RCR
Sbjct: 61 NPYRRGCGTIERCR 73

[Close]

BLAST of Cucsa.178760 vs. Swiss-Prot
Match: RLF20_ARATH (Protein RALF-like 20 OS=Arabidopsis thaliana GN=RALFL20 PE=3 SV=1)

HSP 1 Score: 50.8 bits (120), Expect = 7.8e-06
Identity = 24/73 (32.88%), Postives = 39/73 (53.42%), Query Frame = 1


Query: 1  MIMSKRITLVFIEIFLISFNILTMTDARKFIDYHSIVAGDVSPGCNPKHPELCKLTPANP 60
          M++SK+  +    + +I   +++ T+A+     +  +  D   GC P  P  CK+ PANP
Sbjct: 1  MVLSKKTIMQSFALMIILSIVMSTTEAKTI--GNPAMREDEPKGCPPGSPASCKMQPANP 60

Query: 61 YERGCNKINRCRG 74
          Y+ GC    RCRG
Sbjct: 61 YKPGCEASQRCRG 71

[Close]

BLAST of Cucsa.178760 vs. TrEMBL
Match: A0A0A0L8B1_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_3G426350 PE=4 SV=1)

HSP 1 Score: 121.3 bits (303), Expect = 5.2e-25
Identity = 54/75 (72.00%), Postives = 67/75 (89.33%), Query Frame = 1


Query: 1  MIMSKRITLVFIEIFLISFNILTMTDARKFIDYHSIVAGDVSPGCNPKHPELCKLTPANP 60
          MIMSKR+TLVFI I L+SF+++ +T+ARKFIDY SIVAGDVSPGC+P HPELC++  ANP
Sbjct: 1  MIMSKRMTLVFIGILLVSFDMIMITNARKFIDYGSIVAGDVSPGCSPTHPELCRVKSANP 60

Query: 61 YERGCNKINRCRGGN 76
          Y+RGCN+I+RCR GN
Sbjct: 61 YQRGCNRIDRCREGN 75

[Close]

BLAST of Cucsa.178760 vs. TrEMBL
Match: V4UF46_9ROSI (Uncharacterized protein OS=Citrus clementina GN=CICLE_v10018043mg PE=4 SV=1)

HSP 1 Score: 65.5 bits (158), Expect = 3.4e-08
Identity = 33/75 (44.00%), Postives = 47/75 (62.67%), Query Frame = 1


Query: 1  MIMSKRITLVFIEIFLISFNILTMTDAR--KFIDYHSIVAGDVSPGCNPKHPELCKLTPA 60
          M+MSKR+ +  + + L +  ++ + D    K I Y +I AG  +PGC+P HPELCK+ P 
Sbjct: 1  MMMSKRMMVFGVIMVLTASIVMVVKDGAEAKEIGYGAIGAGG-NPGCSPVHPELCKMEPG 60

Query: 61 NPYERGCNKINRCRG 74
          NPY RGC    +CRG
Sbjct: 61 NPYHRGCENEAQCRG 74

[Close]

BLAST of Cucsa.178760 vs. TrEMBL
Match: A0A067F0E4_CITSI (Uncharacterized protein OS=Citrus sinensis GN=CISIN_1g041712mg PE=4 SV=1)

HSP 1 Score: 65.5 bits (158), Expect = 3.4e-08
Identity = 33/75 (44.00%), Postives = 47/75 (62.67%), Query Frame = 1


Query: 1  MIMSKRITLVFIEIFLISFNILTMTDAR--KFIDYHSIVAGDVSPGCNPKHPELCKLTPA 60
          M+MSKR+ +  + + L +  ++ + D    K I Y +I AG  +PGC+P HPELCK+ P 
Sbjct: 1  MMMSKRMMVFGVIMVLTASIVMVVKDGAEAKEIGYGAIGAGG-NPGCSPVHPELCKMEPG 60

Query: 61 NPYERGCNKINRCRG 74
          NPY RGC    +CRG
Sbjct: 61 NPYHRGCENEAQCRG 74

[Close]

BLAST of Cucsa.178760 vs. TrEMBL
Match: D7KVJ4_ARALL (Putative uncharacterized protein OS=Arabidopsis lyrata subsp. lyrata GN=ARALYDRAFT_893358 PE=4 SV=1)

HSP 1 Score: 60.1 bits (144), Expect = 1.4e-06
Identity = 32/77 (41.56%), Postives = 45/77 (58.44%), Query Frame = 1


Query: 1  MIMSKRITLVFIEIFLISFNILTM--TDARKFIDYHSIVAGDVSPGCNPKHPELCKLTPA 60
          M MSK I ++ + + L+ F  L     +A ++I Y SI  GD +  C+  HP  CK   A
Sbjct: 1  MGMSKSIKVI-LSLALVVFLALASIKVEATRYITYPSINHGDHATHCDKAHPNTCKKKEA 60

Query: 61 NPYERGCNKINRCRGGN 76
          NPY+RGC  + RCRGG+
Sbjct: 61 NPYKRGCEILERCRGGS 76

[Close]

BLAST of Cucsa.178760 vs. TrEMBL
Match: A0A078IYN5_BRANA (BnaCnng31830D protein OS=Brassica napus GN=BnaCnng31830D PE=4 SV=1)

HSP 1 Score: 60.1 bits (144), Expect = 1.4e-06
Identity = 32/77 (41.56%), Postives = 47/77 (61.04%), Query Frame = 1


Query: 1  MIMSKRITLVFIEIFLISFNILTMTDAR-KFIDYHSIVAGDVSPGCNPKHPELCKLTPAN 60
          M MS+ I ++ + +FL+    L  T    ++I+YH++  GD S  C+  HP  CK   AN
Sbjct: 1  MGMSESIKVI-VSLFLVVLLALAATQTESRYINYHAL-HGDHSLICDKAHPSTCKKEEAN 60

Query: 61 PYERGCNKINRCRGGNI 77
          PY RGC KI+RCRG ++
Sbjct: 61 PYTRGCEKIDRCRGQSL 75

[Close]

BLAST of Cucsa.178760 vs. TAIR10
Match: AT1G61566.1 (AT1G61566.1 ralf-like 9)

HSP 1 Score: 67.0 bits (162), Expect = 5.9e-12
Identity = 35/76 (46.05%), Postives = 47/76 (61.84%), Query Frame = 1


Query: 1  MIMSKRITLVFIEIFLISFNILTMT--DARKFIDYHSIVAGDVSPGCNPKHPELCKLTPA 60
          M MSK I ++ + + L+ F  L  T  +A ++I Y +I  GD +  C+  HP  CK   A
Sbjct: 1  MGMSKSIKVI-LSLALVVFLALAATKVEATRYITYPAIDRGDHAVHCDKAHPNTCKKKEA 60

Query: 61 NPYERGCNKINRCRGG 75
          NPY+RGC KINRCRGG
Sbjct: 61 NPYQRGCEKINRCRGG 75

[Close]

BLAST of Cucsa.178760 vs. TAIR10
Match: AT2G22055.1 (AT2G22055.1 RALF-like 15)

HSP 1 Score: 56.2 bits (134), Expect = 1.0e-08
Identity = 31/74 (41.89%), Postives = 41/74 (55.41%), Query Frame = 1


Query: 1  MIMSKRITLVFIEIFLISFNILTMT--DARKFIDYHSIVAGDVSPGCNPKHPELCKLTPA 60
          M MSK I ++ + + LI F  L  T  +A ++I Y  +  GD +  C+  HP  CK   A
Sbjct: 1  MGMSKSIKVI-VSLALILFLALAATKVEATRYISYRGMNHGDHAIHCDKAHPNTCKKQVA 60

Query: 61 NPYERGCNKINRCR 73
          NPY RGC  I RCR
Sbjct: 61 NPYRRGCGTIERCR 73

[Close]

BLAST of Cucsa.178760 vs. TAIR10
Match: AT2G34825.1 (AT2G34825.1 RALF-like 20)

HSP 1 Score: 50.8 bits (120), Expect = 4.4e-07
Identity = 24/73 (32.88%), Postives = 39/73 (53.42%), Query Frame = 1


Query: 1  MIMSKRITLVFIEIFLISFNILTMTDARKFIDYHSIVAGDVSPGCNPKHPELCKLTPANP 60
          M++SK+  +    + +I   +++ T+A+     +  +  D   GC P  P  CK+ PANP
Sbjct: 1  MVLSKKTIMQSFALMIILSIVMSTTEAKTI--GNPAMREDEPKGCPPGSPASCKMQPANP 60

Query: 61 YERGCNKINRCRG 74
          Y+ GC    RCRG
Sbjct: 61 YKPGCEASQRCRG 71

[Close]

BLAST of Cucsa.178760 vs. TAIR10
Match: AT1G23145.1 (AT1G23145.1 RALF-like 2)

HSP 1 Score: 48.5 bits (114), Expect = 2.2e-06
Identity = 28/69 (40.58%), Postives = 36/69 (52.17%), Query Frame = 1


Query: 3  MSKRITLVFIEIFLISFNILTMTDARKFIDYHSIVAGDVSPGCNPKHPELCKLTPANPYE 62
          M  R  LV I +    F  +   +A+K I Y +I   D + GC+PK P  C   P  PY+
Sbjct: 1  MEARHMLVTILLLSFVFMNIMKVEAQKVIGYPAI-GRDGARGCSPKDPS-CPQQPEKPYK 60

Query: 63 RGCNKINRC 72
          RGC KI RC
Sbjct: 61 RGCEKITRC 67

[Close]

BLAST of Cucsa.178760 vs. TAIR10
Match: AT1G35467.1 (AT1G35467.1 RALF-like 5)

HSP 1 Score: 47.0 bits (110), Expect = 6.3e-06
Identity = 25/72 (34.72%), Postives = 37/72 (51.39%), Query Frame = 1


Query: 3  MSKRITLVFIEIFLISFNILTMTDARKFIDYHSIVAGDVSPGCNPKHP--ELCKLTPANP 62
          M K    +F+ + +     +   DA+++I+Y           CNP+ P  +  K TPANP
Sbjct: 1  MLKAQVFMFVTVLVFVCVFINSNDAKRYIEYPPWQKHP----CNPRFPTPDCYKRTPANP 60

Query: 63 YERGCNKINRCR 73
          Y RGC  I+RCR
Sbjct: 61 YRRGCTCISRCR 68

[Close]

BLAST of Cucsa.178760 vs. NCBI nr
Match: gi|700202865|gb|KGN57998.1| (hypothetical protein Csa_3G426350 [Cucumis sativus])

HSP 1 Score: 121.3 bits (303), Expect = 7.5e-25
Identity = 54/75 (72.00%), Postives = 67/75 (89.33%), Query Frame = 1


Query: 1  MIMSKRITLVFIEIFLISFNILTMTDARKFIDYHSIVAGDVSPGCNPKHPELCKLTPANP 60
          MIMSKR+TLVFI I L+SF+++ +T+ARKFIDY SIVAGDVSPGC+P HPELC++  ANP
Sbjct: 1  MIMSKRMTLVFIGILLVSFDMIMITNARKFIDYGSIVAGDVSPGCSPTHPELCRVKSANP 60

Query: 61 YERGCNKINRCRGGN 76
          Y+RGCN+I+RCR GN
Sbjct: 61 YQRGCNRIDRCREGN 75

[Close]

BLAST of Cucsa.178760 vs. NCBI nr
Match: gi|21592626|gb|AAM64575.1| (unknown [Arabidopsis thaliana])

HSP 1 Score: 67.0 bits (162), Expect = 1.7e-08
Identity = 35/76 (46.05%), Postives = 47/76 (61.84%), Query Frame = 1


Query: 1  MIMSKRITLVFIEIFLISFNIL--TMTDARKFIDYHSIVAGDVSPGCNPKHPELCKLTPA 60
          M MSK I ++ + + L+ F  L  T  +A ++I Y +I  GD +  C+  HP  CK   A
Sbjct: 1  MGMSKSIKVI-LSLALVVFLALAGTKVEATRYITYPAIDRGDHAVHCDKAHPNTCKKKQA 60

Query: 61 NPYERGCNKINRCRGG 75
          NPY+RGC KINRCRGG
Sbjct: 61 NPYQRGCEKINRCRGG 75

[Close]

BLAST of Cucsa.178760 vs. NCBI nr
Match: gi|18407238|ref|NP_564779.1| (protein RALF-like 9 [Arabidopsis thaliana])

HSP 1 Score: 67.0 bits (162), Expect = 1.7e-08
Identity = 35/76 (46.05%), Postives = 47/76 (61.84%), Query Frame = 1


Query: 1  MIMSKRITLVFIEIFLISFNILTMT--DARKFIDYHSIVAGDVSPGCNPKHPELCKLTPA 60
          M MSK I ++ + + L+ F  L  T  +A ++I Y +I  GD +  C+  HP  CK   A
Sbjct: 1  MGMSKSIKVI-LSLALVVFLALAATKVEATRYITYPAIDRGDHAVHCDKAHPNTCKKKEA 60

Query: 61 NPYERGCNKINRCRGG 75
          NPY+RGC KINRCRGG
Sbjct: 61 NPYQRGCEKINRCRGG 75

[Close]

BLAST of Cucsa.178760 vs. NCBI nr
Match: gi|567910725|ref|XP_006447676.1| (hypothetical protein CICLE_v10018043mg [Citrus clementina])

HSP 1 Score: 65.5 bits (158), Expect = 4.9e-08
Identity = 33/75 (44.00%), Postives = 47/75 (62.67%), Query Frame = 1


Query: 1  MIMSKRITLVFIEIFLISFNILTMTDAR--KFIDYHSIVAGDVSPGCNPKHPELCKLTPA 60
          M+MSKR+ +  + + L +  ++ + D    K I Y +I AG  +PGC+P HPELCK+ P 
Sbjct: 1  MMMSKRMMVFGVIMVLTASIVMVVKDGAEAKEIGYGAIGAGG-NPGCSPVHPELCKMEPG 60

Query: 61 NPYERGCNKINRCRG 74
          NPY RGC    +CRG
Sbjct: 61 NPYHRGCENEAQCRG 74

[Close]

BLAST of Cucsa.178760 vs. NCBI nr
Match: gi|1009128592|ref|XP_015881314.1| (PREDICTED: protein RALF-like 9 [Ziziphus jujuba])

HSP 1 Score: 60.8 bits (146), Expect = 1.2e-06
Identity = 30/73 (41.10%), Postives = 43/73 (58.90%), Query Frame = 1


Query: 1  MIMSKRITLVFIEI-FLISFNILTMTDARKFIDYHSIVAGDVSPGCNPKHPELCKLTPAN 60
          M + K + +V   +  +I  N++  ++  KF+ Y ++   DV PGC PKHPE CK + AN
Sbjct: 1  MAICKGLLVVIASLQVMIWMNLIGQSEGSKFLQYGALDR-DVIPGCGPKHPEECKKSQAN 60

Query: 61 PYERGCNKINRCR 73
          PY RGC    RCR
Sbjct: 61 PYTRGCLSEERCR 72

The following BLAST results are available for this feature:

BLAST of Cucsa.178760 vs. Swiss-Prot


Match Name	E-value	Identity	Description
RLF9_ARATH	1.0e-10	46.05	Protein RALF-like 9 OS=Arabidopsis thaliana GN=RALFL9 PE=3 SV=1	[more]
RLF15_ARATH	1.8e-07	41.89	Protein RALF-like 15 OS=Arabidopsis thaliana GN=RALFL15 PE=3 SV=1	[more]
RLF20_ARATH	7.8e-06	32.88	Protein RALF-like 20 OS=Arabidopsis thaliana GN=RALFL20 PE=3 SV=1	[more]

BLAST of Cucsa.178760 vs. TrEMBL


Match Name	E-value	Identity	Description
A0A0A0L8B1_CUCSA	5.2e-25	72.00	Uncharacterized protein OS=Cucumis sativus GN=Csa_3G426350 PE=4 SV=1	[more]
V4UF46_9ROSI	3.4e-08	44.00	Uncharacterized protein OS=Citrus clementina GN=CICLE_v10018043mg PE=4 SV=1	[more]
A0A067F0E4_CITSI	3.4e-08	44.00	Uncharacterized protein OS=Citrus sinensis GN=CISIN_1g041712mg PE=4 SV=1	[more]
D7KVJ4_ARALL	1.4e-06	41.56	Putative uncharacterized protein OS=Arabidopsis lyrata subsp. lyrata GN=ARALYDRA...	[more]
A0A078IYN5_BRANA	1.4e-06	41.56	BnaCnng31830D protein OS=Brassica napus GN=BnaCnng31830D PE=4 SV=1	[more]

BLAST of Cucsa.178760 vs. TAIR10


Match Name	E-value	Identity	Description
AT1G61566.1	5.9e-12	46.05	ralf-like 9	[more]
AT2G22055.1	1.0e-08	41.89	RALF-like 15	[more]
AT2G34825.1	4.4e-07	32.88	RALF-like 20	[more]
AT1G23145.1	2.2e-06	40.58	RALF-like 2	[more]
AT1G35467.1	6.3e-06	34.72	RALF-like 5	[more]

BLAST of Cucsa.178760 vs. NCBI nr


Match Name	E-value	Identity	Description
gi\|700202865\|gb\|KGN57998.1\|	7.5e-25	72.00	hypothetical protein Csa_3G426350 [Cucumis sativus]	[more]
gi\|21592626\|gb\|AAM64575.1\|	1.7e-08	46.05	unknown [Arabidopsis thaliana]	[more]
gi\|18407238\|ref\|NP_564779.1\|	1.7e-08	46.05	protein RALF-like 9 [Arabidopsis thaliana]	[more]
gi\|567910725\|ref\|XP_006447676.1\|	4.9e-08	44.00	hypothetical protein CICLE_v10018043mg [Citrus clementina]	[more]
gi\|1009128592\|ref\|XP_015881314.1\|	1.2e-06	41.10	PREDICTED: protein RALF-like 9 [Ziziphus jujuba]	[more]

Annotated Terms

The following terms have been associated with this gene:

Vocabulary: **INTERPRO**
Term	Definition
IPR008801	RALF

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0008150	biological_process
cellular_component	GO:0005575	cellular_component
molecular_function	GO:0003674	molecular_function

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Cucsa.178760.1	Cucsa.178760.1	mRNA

InterPro

Analysis Name: InterPro Annotations of cucumber (Gy14)
Date Performed: 2017-01-17


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR008801	Rapid ALkalinization Factor	PFAM	PF05498	RALF	coord: 26..73 score: 7.4
None	No IPR available	PANTHER	PTHR34270	FAMILY NOT NAMED	coord: 1..75 score: 8.4

Synteny

The following gene(s) are orthologous to this gene:

None

The following gene(s) are paralogous to this gene:

None

The following block(s) are covering this gene:


Gene	Organism	Block
Cucsa.178760	Cucurbita maxima (Rimu)	cgycmaB0509
Cucsa.178760	Cucurbita maxima (Rimu)	cgycmaB0510
Cucsa.178760	Cucurbita moschata (Rifu)	cgycmoB0509
Cucsa.178760	Cucurbita moschata (Rifu)	cgycmoB0510
Cucsa.178760	Wild cucumber (PI 183967)	cgycpiB284
Cucsa.178760	Cucumber (Chinese Long) v2	cgycuB274
Cucsa.178760	Melon (DHL92) v3.5.1	cgymeB320
Cucsa.178760	Watermelon (Charleston Gray)	cgywcgB319
Cucsa.178760	Watermelon (97103) v1	cgywmB341
Cucsa.178760	Cucurbita pepo (Zucchini)	cgycpeB0491
Cucsa.178760	Cucurbita pepo (Zucchini)	cgycpeB0492
Cucsa.178760	Bottle gourd (USVL1VR-Ls)	cgylsiB300
Cucsa.178760	Melon (DHL92) v3.6.1	cgymedB315
Cucsa.178760	Silver-seed gourd	carcgyB0005
Cucsa.178760	Silver-seed gourd	carcgyB0204
Cucsa.178760	Cucumber (Chinese Long) v3	cgycucB287
Cucsa.178760	Watermelon (97103) v2	cgywmbB323
Cucsa.178760	Wax gourd	cgywgoB366