Cucsa.178530 (gene) Cucumber (Gy14) v1 | Cucurbit Genomics Database (CuGenDB)

Overview


Name	Cucsa.178530
Type	gene
Organism	Cucumis sativus (Cucumber (Gy14) v1)
Description	TIR-NBS-LRR disease resistance protein
Location	scaffold01227 : 830739 .. 831589 (-)

Genome Viewer

Gene Expression

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

GGTTGTTTGTTTCTCATGACCGTATCACCATTACTTCTCCATACGCCTAACCATCAAGGTTCCATCAATGGTGGACGCCGCATCATCTCCGACCACCAGACGCTGGACGTACGACGTTTTCTTAAGCTTTAGAGGTGAGGACACTCGTCCGAAGTTCACCAAACACCTTTACCAAGCCCTCGATGCTGCCGGAGTCAACACGTTTCGTGACGACGTCGAACTCCGGCAAGGAGACGCCGTACGCTCAGAGCTCGTGGTAGCAATTAAAAAATCGAGGATAGCGGTCGTGGTGTTCTCCAGTGGCTATGCCGACTCACAGTGGTGCCTAGGGGAGATCGCCGAGATCATGGATTGCCGGACCGCGGATGGCCAACTGGTCCTTCCGATCTTCTATGAGGTTGATCCGTCGGACGTTCGGAAGCAGATGGGGAGGTTTGCGGCCGCGTTTGAGAAGCACGAGAAGAGATATGGTGTAAATTCGGTGGAGGTCCTGCGGTGGAGGGCGGCGCTCACGGAGGCCGCTAGCTTGTCCGGCTGGGACTTGAGGCAGCTAGCCGATGGGTACCTATTATTATTATTATTATTTTCCATCATTTTATTTACCTTTTTCTCATAAAATTGCATTAGCAACGGGCAAAATGGTAATAATAAAAAAAAATCGAAACTATTTACCAAATTTGTTTTTTTTAAAAAAAAAACTCAAATAAATGGGATAGAATTTTGAAAATGTATTAAAGAGAAACAATTTTTTTTAGATACAATTTAAGGTGTGATTTTTTATGAAAAGTTTATGGAATAAAATTAAAGCTGTATGGTTTCCACTTTCTCTGAACATTTTGGTTGAAGTCTGA

mRNA sequence

GGTTGTTTGTTTCTCATGACCGTATCACCATTACTTCTCCATACGCCTAACCATCAAGGTTCCATCAATGGTGGACGCCGCATCATCTCCGACCACCAGACGCTGGACGTACGACGTTTTCTTAAGCTTTAGAGGTGAGGACACTCGTCCGAAGTTCACCAAACACCTTTACCAAGCCCTCGATGCTGCCGGAGTCAACACGTTTCGTGACGACGTCGAACTCCGGCAAGGAGACGCCGTACGCTCAGAGCTCGTGGTAGCAATTAAAAAATCGAGGATAGCGGTCGTGGTGTTCTCCAGTGGCTATGCCGACTCACAGTGGTGCCTAGGGGAGATCGCCGAGATCATGGATTGCCGGACCGCGGATGGCCAACTGGTCCTTCCGATCTTCTATGAGGTTGATCCGTCGGACGTTCGGAAGCAGATGGGGAGGTTTGCGGCCGCGTTTGAGAAGCACGAGAAGAGATATGGTGTAAATTCGGTGGAGGTCCTGCGGTGGAGGGCGGCGCTCACGGAGGCCGCTAGCTTGTCCGGCTGGGACTTGAGGCAGCTAGCCGATGGTTTATGGAATAAAATTAAAGCTGTATGGTTTCCACTTTCTCTGAACATTTTGGTTGAAGTCTGA

Coding sequence (CDS)

ATGGTGGACGCCGCATCATCTCCGACCACCAGACGCTGGACGTACGACGTTTTCTTAAGCTTTAGAGGTGAGGACACTCGTCCGAAGTTCACCAAACACCTTTACCAAGCCCTCGATGCTGCCGGAGTCAACACGTTTCGTGACGACGTCGAACTCCGGCAAGGAGACGCCGTACGCTCAGAGCTCGTGGTAGCAATTAAAAAATCGAGGATAGCGGTCGTGGTGTTCTCCAGTGGCTATGCCGACTCACAGTGGTGCCTAGGGGAGATCGCCGAGATCATGGATTGCCGGACCGCGGATGGCCAACTGGTCCTTCCGATCTTCTATGAGGTTGATCCGTCGGACGTTCGGAAGCAGATGGGGAGGTTTGCGGCCGCGTTTGAGAAGCACGAGAAGAGATATGGTGTAAATTCGGTGGAGGTCCTGCGGTGGAGGGCGGCGCTCACGGAGGCCGCTAGCTTGTCCGGCTGGGACTTGAGGCAGCTAGCCGATGGTTTATGGAATAAAATTAAAGCTGTATGGTTTCCACTTTCTCTGAACATTTTGGTTGAAGTCTGA

Protein sequence

MVDAASSPTTRRWTYDVFLSFRGEDTRPKFTKHLYQALDAAGVNTFRDDVELRQGDAVRSELVVAIKKSRIAVVVFSSGYADSQWCLGEIAEIMDCRTADGQLVLPIFYEVDPSDVRKQMGRFAAAFEKHEKRYGVNSVEVLRWRAALTEAASLSGWDLRQLADGLWNKIKAVWFPLSLNILVEV*

Homology

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BLAST of Cucsa.178530 vs. Swiss-Prot
Match: TMVRN_NICGU (TMV resistance protein N OS=Nicotiana glutinosa GN=N PE=1 SV=1)

HSP 1 Score: 173.3 bits (438), Expect = 2.4e-42
Identity = 83/152 (54.61%), Postives = 107/152 (70.39%), Query Frame = 1


Query: 5   ASSPTTRRWTYDVFLSFRGEDTRPKFTKHLYQALDAAGVNTFRDDVELRQGDAVRSELVV 64
           ASS ++ RW+YDVFLSFRGEDTR  FT HLY+ L+  G+ TF+DD  L  G  +  EL  
Sbjct: 2   ASSSSSSRWSYDVFLSFRGEDTRKTFTSHLYEVLNDKGIKTFQDDKRLEYGATIPGELCK 61

Query: 65  AIKKSRIAVVVFSSGYADSQWCLGEIAEIMDCRTADGQLVLPIFYEVDPSDVRKQMGRFA 124
           AI++S+ A+VVFS  YA S+WCL E+ +IM+C+T   Q V+PIFY+VDPS VR Q   FA
Sbjct: 62  AIEESQFAIVVFSENYATSRWCLNELVKIMECKTRFKQTVIPIFYDVDPSHVRNQKESFA 121

Query: 125 AAFEKHEKRYGVNSVEVLRWRAALTEAASLSG 157
            AFE+HE +Y  +   + RWR AL EAA+L G
Sbjct: 122 KAFEEHETKYKDDVEGIQRWRIALNEAANLKG 153

[Close]

BLAST of Cucsa.178530 vs. Swiss-Prot
Match: Y4117_ARATH (Putative disease resistance protein At4g11170 OS=Arabidopsis thaliana GN=At4g11170 PE=2 SV=1)

HSP 1 Score: 139.0 bits (349), Expect = 5.1e-32
Identity = 71/158 (44.94%), Postives = 98/158 (62.03%), Query Frame = 1


Query: 6   SSPTTRRWTYDVFLSFRGEDTRPKFTKHLYQALDAAGVNTFRDDVELRQGDAVRSELVVA 65
           +S ++  W YDVF SFRGED R  F  HL +  ++ G+ TFRDD  +++   +  EL  A
Sbjct: 2   ASSSSNSWRYDVFPSFRGEDVRNNFLSHLLKEFESKGIVTFRDD-HIKRSHTIGHELRAA 61

Query: 66  IKKSRIAVVVFSSGYADSQWCLGEIAEIMDCRTADGQLVLPIFYEVDPSDVRKQMGRFAA 125
           I++S+I+VV+FS  YA S WCL E+ EIM C+   G  V+P+FY+VDPSD+RKQ G+F  
Sbjct: 62  IRESKISVVLFSENYASSSWCLDELIEIMKCKEEQGLKVMPVFYKVDPSDIRKQTGKFGM 121

Query: 126 AFEKHEKRYGVNSVEVLRWRAALTEAASLSG-----WD 159
           +F   E   G        WR ALT+AA++ G     WD
Sbjct: 122 SF--LETCCGKTEERQHNWRRALTDAANILGDHPQNWD 156

[Close]

BLAST of Cucsa.178530 vs. Swiss-Prot
Match: RPS6R_ARATH (Disease resistance protein RPS6 OS=Arabidopsis thaliana GN=RPS6 PE=1 SV=1)

HSP 1 Score: 136.3 bits (342), Expect = 3.3e-31
Identity = 71/156 (45.51%), Postives = 100/156 (64.10%), Query Frame = 1


Query: 4   AASSPTTRRWTYDVFLSFRGEDTRPKFTKHLYQALDAAGVNTFRDDVELRQGDAVRSELV 63
           ++SS ++R W+Y VF SF GED R  F  H  + LD   + +F+D+ E+ +  ++  EL 
Sbjct: 3   SSSSSSSRNWSYHVFPSFSGEDVRNTFLSHFLKELDRKLIISFKDN-EIERSQSLDPELK 62

Query: 64  VAIKKSRIAVVVFSSGYADSQWCLGEIAEIMDCRTADGQLVLPIFYEVDPSDVRKQMGRF 123
             I+ SRIAVVVFS  YA S WCL E+ EI+ C+   GQLV+PIFY +DPS VRKQ G F
Sbjct: 63  HGIRNSRIAVVVFSKTYASSSWCLNELLEIVKCKKEFGQLVIPIFYNLDPSHVRKQTGDF 122

Query: 124 AAAFEKHEKRYGVNSVEVLRWRAALTEAASLSGWDL 160
              FEK  +   V+  E +RW+ ALT+ A++ G+ +
Sbjct: 123 GKIFEKTCRNKTVD--EKIRWKEALTDVANILGYHI 155

[Close]

BLAST of Cucsa.178530 vs. Swiss-Prot
Match: RPS6C_ARATH (Disease resistance protein RPS6 OS=Arabidopsis thaliana GN=RPS6 PE=1 SV=1)

HSP 1 Score: 136.3 bits (342), Expect = 3.3e-31
Identity = 71/156 (45.51%), Postives = 100/156 (64.10%), Query Frame = 1


Query: 4   AASSPTTRRWTYDVFLSFRGEDTRPKFTKHLYQALDAAGVNTFRDDVELRQGDAVRSELV 63
           ++SS ++R W+Y VF SF GED R  F  H  + LD   + +F+D+ E+ +  ++  EL 
Sbjct: 3   SSSSSSSRNWSYHVFPSFSGEDVRNTFLSHFLKELDRKLIISFKDN-EIERSQSLDPELK 62

Query: 64  VAIKKSRIAVVVFSSGYADSQWCLGEIAEIMDCRTADGQLVLPIFYEVDPSDVRKQMGRF 123
             I+ SRIAVVVFS  YA S WCL E+ EI+ C+   GQLV+PIFY +DPS VRKQ G F
Sbjct: 63  HGIRNSRIAVVVFSKTYASSSWCLNELLEIVKCKKEFGQLVIPIFYNLDPSHVRKQTGDF 122

Query: 124 AAAFEKHEKRYGVNSVEVLRWRAALTEAASLSGWDL 160
              FEK  +   V+  E +RW+ ALT+ A++ G+ +
Sbjct: 123 GKIFEKTCRNKTVD--EKIRWKEALTDVANILGYHI 155

[Close]

BLAST of Cucsa.178530 vs. Swiss-Prot
Match: VICTR_ARATH (Protein VARIATION IN COMPOUND TRIGGERED ROOT growth response OS=Arabidopsis thaliana GN=VICTR PE=1 SV=1)

HSP 1 Score: 134.0 bits (336), Expect = 1.6e-30
Identity = 72/155 (46.45%), Postives = 102/155 (65.81%), Query Frame = 1


Query: 5   ASSPTTRRWTYDVFLSFRGEDTRPKFTKHLYQALDAAGVNTFRDDVELRQGDAVRSELVV 64
           ASS ++R W YDVFLSF G+D R  F  H  + LD   ++ FRD+ E+ +  ++  +L  
Sbjct: 2   ASSSSSRNWVYDVFLSFSGKDVRVTFRSHFLKELDRKLISAFRDN-EIERSHSLWPDLEQ 61

Query: 65  AIKKSRIAVVVFSSGYADSQWCLGEIAEIMDCRTADGQLVLPIFYEVDPSDVRKQMGRFA 124
           AIK SRIAVVVFS  YA S WCL E+ EI++C   + ++++P+FY VDPS VR Q+G F 
Sbjct: 62  AIKDSRIAVVVFSKNYASSSWCLNELLEIVNC---NDKIIIPVFYGVDPSQVRYQIGEFG 121

Query: 125 AAFEKHEKRYGVNSVEVL-RWRAALTEAASLSGWD 159
           + FEK  KR    + EV  +W+ ALT+ A++ G+D
Sbjct: 122 SIFEKTCKR---QTEEVKNQWKKALTDVANMLGFD 149

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BLAST of Cucsa.178530 vs. TrEMBL
Match: A0A0A0LJJ7_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_2G074250 PE=4 SV=1)

HSP 1 Score: 332.0 bits (850), Expect = 4.6e-88
Identity = 165/165 (100.00%), Postives = 165/165 (100.00%), Query Frame = 1


Query: 1   MVDAASSPTTRRWTYDVFLSFRGEDTRPKFTKHLYQALDAAGVNTFRDDVELRQGDAVRS 60
           MVDAASSPTTRRWTYDVFLSFRGEDTRPKFTKHLYQALDAAGVNTFRDDVELRQGDAVRS
Sbjct: 1   MVDAASSPTTRRWTYDVFLSFRGEDTRPKFTKHLYQALDAAGVNTFRDDVELRQGDAVRS 60

Query: 61  ELVVAIKKSRIAVVVFSSGYADSQWCLGEIAEIMDCRTADGQLVLPIFYEVDPSDVRKQM 120
           ELVVAIKKSRIAVVVFSSGYADSQWCLGEIAEIMDCRTADGQLVLPIFYEVDPSDVRKQM
Sbjct: 61  ELVVAIKKSRIAVVVFSSGYADSQWCLGEIAEIMDCRTADGQLVLPIFYEVDPSDVRKQM 120

Query: 121 GRFAAAFEKHEKRYGVNSVEVLRWRAALTEAASLSGWDLRQLADG 166
           GRFAAAFEKHEKRYGVNSVEVLRWRAALTEAASLSGWDLRQLADG
Sbjct: 121 GRFAAAFEKHEKRYGVNSVEVLRWRAALTEAASLSGWDLRQLADG 165

[Close]

BLAST of Cucsa.178530 vs. TrEMBL
Match: M5XI94_PRUPE (Uncharacterized protein OS=Prunus persica GN=PRUPE_ppa022336mg PE=4 SV=1)

HSP 1 Score: 208.8 bits (530), Expect = 5.8e-51
Identity = 101/157 (64.33%), Postives = 124/157 (78.98%), Query Frame = 1


Query: 9   TTRRWTYDVFLSFRGEDTRPKFTKHLYQALDAAGVNTFRDDVELRQGDAVRSELVVAIKK 68
           ++++W YDVFLSFRGEDTR  FT HLY AL  AG+N F DD ELR+G+ + +ELV AI+ 
Sbjct: 11  SSKQWIYDVFLSFRGEDTRKNFTGHLYMALKEAGINAFIDDNELRRGEDITAELVQAIQG 70

Query: 69  SRIAVVVFSSGYADSQWCLGEIAEIMDCRTADGQLVLPIFYEVDPSDVRKQMGRFAAAFE 128
           SRI+V+VFS  YADS WCL E+ +IM+CR    Q+VLPIFY+VDPSDVRKQ G F  AFE
Sbjct: 71  SRISVIVFSRRYADSGWCLEELVKIMECRRTVRQMVLPIFYDVDPSDVRKQNGCFGQAFE 130

Query: 129 KHEKRYGVNSVEVLRWRAALTEAASLSGWDLRQLADG 166
           KHE+R+ +   +VLRWR+ALTEAA+LSGWDLR  ADG
Sbjct: 131 KHEERFLLEVDKVLRWRSALTEAANLSGWDLRNTADG 167

[Close]

BLAST of Cucsa.178530 vs. TrEMBL
Match: U5FF18_POPTR (Uncharacterized protein (Fragment) OS=Populus trichocarpa GN=POPTR_0019s01570g PE=4 SV=1)

HSP 1 Score: 203.0 bits (515), Expect = 3.2e-49
Identity = 99/164 (60.37%), Postives = 126/164 (76.83%), Query Frame = 1


Query: 4   AASSPTTRRWTYDVFLSFRGEDTRPKFTKHLYQALDAAGVNTFRDDVELRQGDAVRSELV 63
           ++S  +++ W YDVFLSFRGEDTR  FT HLY A   AG+NTFRDD ELR+G+ + +EL+
Sbjct: 6   SSSHSSSKPWIYDVFLSFRGEDTRKNFTDHLYFAFKDAGINTFRDDNELRRGEDISTELL 65

Query: 64  VAIKKSRIAVVVFSSGYADSQWCLGEIAEIMDCRTADGQLVLPIFYEVDPSDVRKQMGRF 123
            AI+KSRI+V+VFS  YA+S+WCL E+ +IM+CR +  QLV PIFY+VDPSDVRKQ G F
Sbjct: 66  QAIQKSRISVIVFSENYANSRWCLEELVKIMECRRSCRQLVFPIFYDVDPSDVRKQTGSF 125

Query: 124 AAAFEKHEKRY--GVNSVEVLRWRAALTEAASLSGWDLRQLADG 166
           A AF  HE+R+    +  +V  WR ALTEAA+LSGWDLR +ADG
Sbjct: 126 AKAFAGHEERFVLQTDKGKVATWRMALTEAANLSGWDLRNVADG 169

[Close]

BLAST of Cucsa.178530 vs. TrEMBL
Match: W9QLS2_9ROSA (TMV resistance protein N OS=Morus notabilis GN=L484_016858 PE=4 SV=1)

HSP 1 Score: 202.6 bits (514), Expect = 4.2e-49
Identity = 101/161 (62.73%), Postives = 124/161 (77.02%), Query Frame = 1


Query: 5   ASSPTTRRWTYDVFLSFRGEDTRPKFTKHLYQALDAAGVNTFRDDVELRQGDAVRSELVV 64
           +SS ++   TYDVFLSFRGEDTR  FT HLY AL  AGVN F DD EL +G+ +  ELV 
Sbjct: 30  SSSSSSNARTYDVFLSFRGEDTRKTFTGHLYSALTDAGVNAFIDDRELPKGENIPEELVR 89

Query: 65  AIKKSRIAVVVFSSGYADSQWCLGEIAEIMDCRTADGQLVLPIFYEVDPSDVRKQMGRFA 124
           AI+ SRI+VVVFS  YADS WCL E+ +IM+CR    Q+++P+FY+VDPSDVRKQ G F+
Sbjct: 90  AIQGSRISVVVFSRNYADSSWCLEELVKIMECRRTVRQMMIPVFYDVDPSDVRKQTGIFS 149

Query: 125 AAFEKHEKRYGVNSVEVLRWRAALTEAASLSGWDLRQLADG 166
            +FEKHEK +  +S +VLRWR+ALTEAA+LSGWDLR  ADG
Sbjct: 150 KSFEKHEKWFLSDSEKVLRWRSALTEAANLSGWDLRNTADG 190

[Close]

BLAST of Cucsa.178530 vs. TrEMBL
Match: U7DZ20_POPTR (Uncharacterized protein OS=Populus trichocarpa GN=POPTR_0182s00230g PE=4 SV=1)

HSP 1 Score: 201.4 bits (511), Expect = 9.3e-49
Identity = 99/164 (60.37%), Postives = 125/164 (76.22%), Query Frame = 1


Query: 4   AASSPTTRRWTYDVFLSFRGEDTRPKFTKHLYQALDAAGVNTFRDDVELRQGDAVRSELV 63
           ++S   ++ W YDVFLSFRGEDTR  FT HLY A   AG+NTFRDD ELR+G+ + +EL+
Sbjct: 6   SSSHSFSKPWIYDVFLSFRGEDTRKNFTDHLYFAFKDAGINTFRDDNELRRGEDISTELL 65

Query: 64  VAIKKSRIAVVVFSSGYADSQWCLGEIAEIMDCRTADGQLVLPIFYEVDPSDVRKQMGRF 123
            AI+KSRI+V+VFS  YA+S+WCL E+ +IM+CR +  QLV PIFY+VDPSDVRKQ G F
Sbjct: 66  QAIQKSRISVIVFSRNYANSRWCLEELVKIMECRRSCRQLVFPIFYDVDPSDVRKQTGSF 125

Query: 124 AAAFEKHEKRY--GVNSVEVLRWRAALTEAASLSGWDLRQLADG 166
           A AF  HE+R+    +  +V  WR ALTEAA+LSGWDLR +ADG
Sbjct: 126 AEAFAGHEERFVLQTDKGKVATWRMALTEAANLSGWDLRNVADG 169

[Close]

BLAST of Cucsa.178530 vs. TAIR10
Match: AT5G36930.2 (AT5G36930.2 Disease resistance protein (TIR-NBS-LRR class) family)

HSP 1 Score: 149.8 bits (377), Expect = 1.6e-36
Identity = 71/150 (47.33%), Postives = 100/150 (66.67%), Query Frame = 1


Query: 12  RWTYDVFLSFRGEDTRPKFTKHLYQALDAAGVNTFRDDVELRQGDAVRSELVVAIKKSRI 71
           RWTYDVF+SFRG D R  F  HLY +L   G++TF DDVEL++G+ +  EL+ AI+ S+I
Sbjct: 14  RWTYDVFVSFRGADVRKNFLSHLYDSLRRCGISTFMDDVELQRGEYISPELLNAIETSKI 73

Query: 72  AVVVFSSGYADSQWCLGEIAEIMDC-RTADGQLVLPIFYEVDPSDVRKQMGRFAAAFEKH 131
            +VV +  YA S WCL E+  IM   +     +V PIF  VDPSD+R Q G +A +F KH
Sbjct: 74  LIVVLTKDYASSAWCLDELVHIMKSHKNNPSHMVFPIFLYVDPSDIRWQQGSYAKSFSKH 133

Query: 132 EKRYGVNSVEVLRWRAALTEAASLSGWDLR 161
           +  + +N ++   WR ALT+ A++SGWD++
Sbjct: 134 KNSHPLNKLK--DWREALTKVANISGWDIK 161

[Close]

BLAST of Cucsa.178530 vs. TAIR10
Match: AT5G17680.1 (AT5G17680.1 disease resistance protein (TIR-NBS-LRR class), putative)

HSP 1 Score: 141.4 bits (355), Expect = 5.8e-34
Identity = 72/161 (44.72%), Postives = 100/161 (62.11%), Query Frame = 1


Query: 4   AASSPTTRRWTYDVFLSFRGEDTRPKFTKHLYQALDAAGVNTFRDDVELRQGDAVRSELV 63
           ++SS ++  W  DVF+SFRGED R  F  HL+   D  G+  FRDD++L++G ++  EL+
Sbjct: 7   SSSSSSSTVWKTDVFVSFRGEDVRKTFVSHLFCEFDRMGIKAFRDDLDLQRGKSISPELI 66

Query: 64  VAIKKSRIAVVVFSSGYADSQWCLGEIAEIMDCRTADGQLVLPIFYEVDPSDVRKQMGRF 123
            AIK SR A+VV S  YA S WCL E+ +IM+C   +   ++PIFYEVDPSDVR+Q G F
Sbjct: 67  DAIKGSRFAIVVVSRNYAASSWCLDELLKIMEC---NKDTIVPIFYEVDPSDVRRQRGSF 126

Query: 124 AAAFEKHEKRYGVNSVEVLRWRAALTEAASLSGWDLRQLAD 165
               E H  +  V      +W+ AL + A++SG D R   D
Sbjct: 127 GEDVESHSDKEKVG-----KWKEALKKLAAISGEDSRNWDD 159

[Close]

BLAST of Cucsa.178530 vs. TAIR10
Match: AT1G17615.1 (AT1G17615.1 Disease resistance protein (TIR-NBS class))

HSP 1 Score: 141.0 bits (354), Expect = 7.6e-34
Identity = 75/164 (45.73%), Postives = 101/164 (61.59%), Query Frame = 1


Query: 1   MVDAASSPTTRRWTYDVFLSFRGEDTRPKFTKHLYQALDAAGVNTFRDDVELRQGDAVRS 60
           M  ++SS +     +DVFLSFRG+DTR  F   LY+ L   G+ TF+DDVEL+ G  + S
Sbjct: 1   MYSSSSSSSATPQKFDVFLSFRGKDTRRTFISFLYKELIEMGIRTFKDDVELQSGRRIAS 60

Query: 61  ELVVAIKKSRIAVVVFSSGYADSQWCLGEIAEIMDCRTADGQLVLPIFYEVDPSDVRKQM 120
           +L+ AI+ S+IAVV+ S  Y+ S WCL E+  IMD       +V+PIFY V+P+ VR+Q+
Sbjct: 61  DLLAAIENSKIAVVIISKNYSASPWCLQELVMIMDVEKKGSIIVMPIFYNVEPAHVRRQI 120

Query: 121 GRFAAAFEKHEKRYGVNSVEVLRWRAALTEAASLSGWDLRQLAD 165
            + A  F KHE R   N   V+ WR ALT  AS+SG   R   D
Sbjct: 121 EQVAKQFRKHEGR--ENYETVVSWRQALTNLASISGHCSRDCED 162

[Close]

BLAST of Cucsa.178530 vs. TAIR10
Match: AT1G72890.2 (AT1G72890.2 Disease resistance protein (TIR-NBS class))

HSP 1 Score: 139.8 bits (351), Expect = 1.7e-33
Identity = 77/155 (49.68%), Postives = 99/155 (63.87%), Query Frame = 1


Query: 2   VDAASSPTTRRWTYDVFLSFRGEDTRPKFTKHLYQALDAAGVNTFRDDVELRQGDAVRSE 61
           + ++SS +     +DVFLSFRG+DTR  F   LY+AL + G+ TF+DD EL +G  +  E
Sbjct: 26  LSSSSSSSFTPQKFDVFLSFRGKDTRKNFVSFLYKALVSKGIRTFKDDEELERGRPIPPE 85

Query: 62  LVVAIKKSRIAVVVFSSGYADSQWCLGEIAEIMDCRTADGQLVLPIFYEVDPSDVRKQMG 121
           L  AIK SRIAVVV S  Y  S WCL E+ EI+         V+PIFYE++PSDVR+Q G
Sbjct: 86  LRQAIKGSRIAVVVVSVTYPASSWCLEELREILKLEKLGLLTVIPIFYEINPSDVRRQSG 145

Query: 122 RFAAAFEKHEKRYGVNSVEVLRWRAALTEAASLSG 157
             +  F+KHEKR     V+   WR ALT+ ASLSG
Sbjct: 146 VVSKQFKKHEKRQSRERVK--SWREALTKLASLSG 178

[Close]

BLAST of Cucsa.178530 vs. TAIR10
Match: AT4G11170.1 (AT4G11170.1 Disease resistance protein (TIR-NBS-LRR class) family)

HSP 1 Score: 139.0 bits (349), Expect = 2.9e-33
Identity = 71/158 (44.94%), Postives = 98/158 (62.03%), Query Frame = 1


Query: 6   SSPTTRRWTYDVFLSFRGEDTRPKFTKHLYQALDAAGVNTFRDDVELRQGDAVRSELVVA 65
           +S ++  W YDVF SFRGED R  F  HL +  ++ G+ TFRDD  +++   +  EL  A
Sbjct: 2   ASSSSNSWRYDVFPSFRGEDVRNNFLSHLLKEFESKGIVTFRDD-HIKRSHTIGHELRAA 61

Query: 66  IKKSRIAVVVFSSGYADSQWCLGEIAEIMDCRTADGQLVLPIFYEVDPSDVRKQMGRFAA 125
           I++S+I+VV+FS  YA S WCL E+ EIM C+   G  V+P+FY+VDPSD+RKQ G+F  
Sbjct: 62  IRESKISVVLFSENYASSSWCLDELIEIMKCKEEQGLKVMPVFYKVDPSDIRKQTGKFGM 121

Query: 126 AFEKHEKRYGVNSVEVLRWRAALTEAASLSG-----WD 159
           +F   E   G        WR ALT+AA++ G     WD
Sbjct: 122 SF--LETCCGKTEERQHNWRRALTDAANILGDHPQNWD 156

[Close]

BLAST of Cucsa.178530 vs. NCBI nr
Match: gi|778674089|ref|XP_004152878.2| (PREDICTED: TMV resistance protein N-like [Cucumis sativus])

HSP 1 Score: 332.0 bits (850), Expect = 6.6e-88
Identity = 165/165 (100.00%), Postives = 165/165 (100.00%), Query Frame = 1


Query: 1   MVDAASSPTTRRWTYDVFLSFRGEDTRPKFTKHLYQALDAAGVNTFRDDVELRQGDAVRS 60
           MVDAASSPTTRRWTYDVFLSFRGEDTRPKFTKHLYQALDAAGVNTFRDDVELRQGDAVRS
Sbjct: 1   MVDAASSPTTRRWTYDVFLSFRGEDTRPKFTKHLYQALDAAGVNTFRDDVELRQGDAVRS 60

Query: 61  ELVVAIKKSRIAVVVFSSGYADSQWCLGEIAEIMDCRTADGQLVLPIFYEVDPSDVRKQM 120
           ELVVAIKKSRIAVVVFSSGYADSQWCLGEIAEIMDCRTADGQLVLPIFYEVDPSDVRKQM
Sbjct: 61  ELVVAIKKSRIAVVVFSSGYADSQWCLGEIAEIMDCRTADGQLVLPIFYEVDPSDVRKQM 120

Query: 121 GRFAAAFEKHEKRYGVNSVEVLRWRAALTEAASLSGWDLRQLADG 166
           GRFAAAFEKHEKRYGVNSVEVLRWRAALTEAASLSGWDLRQLADG
Sbjct: 121 GRFAAAFEKHEKRYGVNSVEVLRWRAALTEAASLSGWDLRQLADG 165

[Close]

BLAST of Cucsa.178530 vs. NCBI nr
Match: gi|700206145|gb|KGN61264.1| (hypothetical protein Csa_2G074250 [Cucumis sativus])

HSP 1 Score: 332.0 bits (850), Expect = 6.6e-88
Identity = 165/165 (100.00%), Postives = 165/165 (100.00%), Query Frame = 1


Query: 1   MVDAASSPTTRRWTYDVFLSFRGEDTRPKFTKHLYQALDAAGVNTFRDDVELRQGDAVRS 60
           MVDAASSPTTRRWTYDVFLSFRGEDTRPKFTKHLYQALDAAGVNTFRDDVELRQGDAVRS
Sbjct: 1   MVDAASSPTTRRWTYDVFLSFRGEDTRPKFTKHLYQALDAAGVNTFRDDVELRQGDAVRS 60

Query: 61  ELVVAIKKSRIAVVVFSSGYADSQWCLGEIAEIMDCRTADGQLVLPIFYEVDPSDVRKQM 120
           ELVVAIKKSRIAVVVFSSGYADSQWCLGEIAEIMDCRTADGQLVLPIFYEVDPSDVRKQM
Sbjct: 61  ELVVAIKKSRIAVVVFSSGYADSQWCLGEIAEIMDCRTADGQLVLPIFYEVDPSDVRKQM 120

Query: 121 GRFAAAFEKHEKRYGVNSVEVLRWRAALTEAASLSGWDLRQLADG 166
           GRFAAAFEKHEKRYGVNSVEVLRWRAALTEAASLSGWDLRQLADG
Sbjct: 121 GRFAAAFEKHEKRYGVNSVEVLRWRAALTEAASLSGWDLRQLADG 165

[Close]

BLAST of Cucsa.178530 vs. NCBI nr
Match: gi|659082460|ref|XP_008441852.1| (PREDICTED: TMV resistance protein N-like [Cucumis melo])

HSP 1 Score: 299.3 bits (765), Expect = 4.7e-78
Identity = 149/165 (90.30%), Postives = 152/165 (92.12%), Query Frame = 1


Query: 1   MVDAASSPTTRRWTYDVFLSFRGEDTRPKFTKHLYQALDAAGVNTFRDDVELRQGDAVRS 60
           MVD  SS TTRRW YDVFLSFRGEDTR  FTKHLY ALD AGVNTFRDDVELRQGDAV S
Sbjct: 1   MVDVPSSSTTRRWMYDVFLSFRGEDTRQNFTKHLYDALDTAGVNTFRDDVELRQGDAVGS 60

Query: 61  ELVVAIKKSRIAVVVFSSGYADSQWCLGEIAEIMDCRTADGQLVLPIFYEVDPSDVRKQM 120
           ELVVAIKKSRIAVVVFS GYADSQWCLGEIAEIMDCRT +GQLVLPIFYEVDPSDVRKQ 
Sbjct: 61  ELVVAIKKSRIAVVVFSDGYADSQWCLGEIAEIMDCRTVEGQLVLPIFYEVDPSDVRKQK 120

Query: 121 GRFAAAFEKHEKRYGVNSVEVLRWRAALTEAASLSGWDLRQLADG 166
           GRFAAAFEKHEKR+GV+SVEVLRWRAAL EAASLSGWDLRQLADG
Sbjct: 121 GRFAAAFEKHEKRFGVDSVEVLRWRAALREAASLSGWDLRQLADG 165

[Close]

BLAST of Cucsa.178530 vs. NCBI nr
Match: gi|596240595|ref|XP_007224475.1| (hypothetical protein PRUPE_ppa022336mg [Prunus persica])

HSP 1 Score: 208.8 bits (530), Expect = 8.4e-51
Identity = 101/157 (64.33%), Postives = 124/157 (78.98%), Query Frame = 1


Query: 9   TTRRWTYDVFLSFRGEDTRPKFTKHLYQALDAAGVNTFRDDVELRQGDAVRSELVVAIKK 68
           ++++W YDVFLSFRGEDTR  FT HLY AL  AG+N F DD ELR+G+ + +ELV AI+ 
Sbjct: 11  SSKQWIYDVFLSFRGEDTRKNFTGHLYMALKEAGINAFIDDNELRRGEDITAELVQAIQG 70

Query: 69  SRIAVVVFSSGYADSQWCLGEIAEIMDCRTADGQLVLPIFYEVDPSDVRKQMGRFAAAFE 128
           SRI+V+VFS  YADS WCL E+ +IM+CR    Q+VLPIFY+VDPSDVRKQ G F  AFE
Sbjct: 71  SRISVIVFSRRYADSGWCLEELVKIMECRRTVRQMVLPIFYDVDPSDVRKQNGCFGQAFE 130

Query: 129 KHEKRYGVNSVEVLRWRAALTEAASLSGWDLRQLADG 166
           KHE+R+ +   +VLRWR+ALTEAA+LSGWDLR  ADG
Sbjct: 131 KHEERFLLEVDKVLRWRSALTEAANLSGWDLRNTADG 167

[Close]

BLAST of Cucsa.178530 vs. NCBI nr
Match: gi|743905830|ref|XP_011046325.1| (PREDICTED: TMV resistance protein N-like isoform X2 [Populus euphratica])

HSP 1 Score: 205.3 bits (521), Expect = 9.3e-50
Identity = 100/164 (60.98%), Postives = 128/164 (78.05%), Query Frame = 1


Query: 4   AASSPTTRRWTYDVFLSFRGEDTRPKFTKHLYQALDAAGVNTFRDDVELRQGDAVRSELV 63
           A+S  +++ W YDVFLSFRGEDTR  FT HLY AL  AG+NTFRDD ELR+G+ + +EL+
Sbjct: 8   ASSYSSSKPWIYDVFLSFRGEDTRKNFTDHLYFALKDAGINTFRDDNELRRGEDISTELL 67

Query: 64  VAIKKSRIAVVVFSSGYADSQWCLGEIAEIMDCRTADGQLVLPIFYEVDPSDVRKQMGRF 123
            AI+KSRI+V+VFS  YA+S+WCL E+ +IM+CR +  QLV PIFY+VDPSDVRKQ G F
Sbjct: 68  QAIQKSRISVIVFSRNYANSRWCLEELVKIMECRRSCRQLVFPIFYDVDPSDVRKQTGSF 127

Query: 124 AAAFEKHEKRY--GVNSVEVLRWRAALTEAASLSGWDLRQLADG 166
           A AF +H++R+    +  +V  WR ALTEAA+LSGWDLR +ADG
Sbjct: 128 AEAFARHDERFVLQTDKGKVATWRMALTEAANLSGWDLRNVADG 171

The following BLAST results are available for this feature:

BLAST of Cucsa.178530 vs. Swiss-Prot


Match Name	E-value	Identity	Description
TMVRN_NICGU	2.4e-42	54.61	TMV resistance protein N OS=Nicotiana glutinosa GN=N PE=1 SV=1	[more]
Y4117_ARATH	5.1e-32	44.94	Putative disease resistance protein At4g11170 OS=Arabidopsis thaliana GN=At4g111...	[more]
RPS6R_ARATH	3.3e-31	45.51	Disease resistance protein RPS6 OS=Arabidopsis thaliana GN=RPS6 PE=1 SV=1	[more]
RPS6C_ARATH	3.3e-31	45.51	Disease resistance protein RPS6 OS=Arabidopsis thaliana GN=RPS6 PE=1 SV=1	[more]
VICTR_ARATH	1.6e-30	46.45	Protein VARIATION IN COMPOUND TRIGGERED ROOT growth response OS=Arabidopsis thal...	[more]

BLAST of Cucsa.178530 vs. TrEMBL


Match Name	E-value	Identity	Description
A0A0A0LJJ7_CUCSA	4.6e-88	100.00	Uncharacterized protein OS=Cucumis sativus GN=Csa_2G074250 PE=4 SV=1	[more]
M5XI94_PRUPE	5.8e-51	64.33	Uncharacterized protein OS=Prunus persica GN=PRUPE_ppa022336mg PE=4 SV=1	[more]
U5FF18_POPTR	3.2e-49	60.37	Uncharacterized protein (Fragment) OS=Populus trichocarpa GN=POPTR_0019s01570g P...	[more]
W9QLS2_9ROSA	4.2e-49	62.73	TMV resistance protein N OS=Morus notabilis GN=L484_016858 PE=4 SV=1	[more]
U7DZ20_POPTR	9.3e-49	60.37	Uncharacterized protein OS=Populus trichocarpa GN=POPTR_0182s00230g PE=4 SV=1	[more]

BLAST of Cucsa.178530 vs. TAIR10


Match Name	E-value	Identity	Description
AT5G36930.2	1.6e-36	47.33	Disease resistance protein (TIR-NBS-LRR class) family	[more]
AT5G17680.1	5.8e-34	44.72	disease resistance protein (TIR-NBS-LRR class), putative	[more]
AT1G17615.1	7.6e-34	45.73	Disease resistance protein (TIR-NBS class)	[more]
AT1G72890.2	1.7e-33	49.68	Disease resistance protein (TIR-NBS class)	[more]
AT4G11170.1	2.9e-33	44.94	Disease resistance protein (TIR-NBS-LRR class) family	[more]

BLAST of Cucsa.178530 vs. NCBI nr


Match Name	E-value	Identity	Description
gi\|778674089\|ref\|XP_004152878.2\|	6.6e-88	100.00	PREDICTED: TMV resistance protein N-like [Cucumis sativus]	[more]
gi\|700206145\|gb\|KGN61264.1\|	6.6e-88	100.00	hypothetical protein Csa_2G074250 [Cucumis sativus]	[more]
gi\|659082460\|ref\|XP_008441852.1\|	4.7e-78	90.30	PREDICTED: TMV resistance protein N-like [Cucumis melo]	[more]
gi\|596240595\|ref\|XP_007224475.1\|	8.4e-51	64.33	hypothetical protein PRUPE_ppa022336mg [Prunus persica]	[more]
gi\|743905830\|ref\|XP_011046325.1\|	9.3e-50	60.98	PREDICTED: TMV resistance protein N-like isoform X2 [Populus euphratica]	[more]

Annotated Terms

The following terms have been associated with this gene:

Vocabulary: **INTERPRO**
Term	Definition
IPR000157	TIR_dom

Vocabulary: **Molecular Function**
Term	Definition
GO:0005515	protein binding

Vocabulary: **Biological Process**
Term	Definition
GO:0007165	signal transduction

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0007165	signal transduction
cellular_component	GO:0005575	cellular_component
molecular_function	GO:0043531	ADP binding
molecular_function	GO:0005515	protein binding

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Cucsa.178530.1	Cucsa.178530.1	mRNA

InterPro

Analysis Name: InterPro Annotations of cucumber (Gy14)
Date Performed: 2017-01-17


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR000157	Toll/interleukin-1 receptor homology (TIR) domain	GENE3D	G3DSA:3.40.50.10140		coord: 11..131 score: 7.0
IPR000157	Toll/interleukin-1 receptor homology (TIR) domain	PFAM	PF01582	TIR	coord: 15..170 score: 4.5
IPR000157	Toll/interleukin-1 receptor homology (TIR) domain	SMART	SM00255	till_3	coord: 14..154 score: 2.8
IPR000157	Toll/interleukin-1 receptor homology (TIR) domain	PROFILE	PS50104	TIR	coord: 13..154 score: 28
IPR000157	Toll/interleukin-1 receptor homology (TIR) domain	unknown	SSF52200	Toll/Interleukin receptor TIR domain	coord: 6..157 score: 2.88
None	No IPR available	PANTHER	PTHR11017	LEUCINE-RICH REPEAT-CONTAINING PROTEIN	coord: 6..161 score: 1.6
None	No IPR available	PANTHER	PTHR11017:SF169	DISEASE RESISTANCE PROTEIN-RELATED	coord: 6..161 score: 1.6

Synteny

The following gene(s) are orthologous to this gene:


Gene	Orthologue	Organism	Block
Cucsa.178530	MELO3C031443.2	Melon (DHL92) v3.6.1	cgymedB315
Cucsa.178530	CsaV3_2G009690	Cucumber (Chinese Long) v3	cgycucB287
Cucsa.178530	Cla97C11G216940	Watermelon (97103) v2	cgywmbB323
Cucsa.178530	Bhi06G000967	Wax gourd	cgywgoB368

The following gene(s) are paralogous to this gene:

None

The following block(s) are covering this gene:


Gene	Organism	Block
Cucsa.178530	Cucurbita pepo (Zucchini)	cgycpeB0491
Cucsa.178530	Cucurbita pepo (Zucchini)	cgycpeB0492
Cucsa.178530	Bottle gourd (USVL1VR-Ls)	cgylsiB300
Cucsa.178530	Silver-seed gourd	carcgyB0204
Cucsa.178530	Silver-seed gourd	carcgyB0805
Cucsa.178530	Wax gourd	cgywgoB366
Cucsa.178530	Cucurbita maxima (Rimu)	cgycmaB0509
Cucsa.178530	Cucurbita maxima (Rimu)	cgycmaB0510
Cucsa.178530	Cucurbita moschata (Rifu)	cgycmoB0509
Cucsa.178530	Cucurbita moschata (Rifu)	cgycmoB0510
Cucsa.178530	Wild cucumber (PI 183967)	cgycpiB284
Cucsa.178530	Cucumber (Chinese Long) v2	cgycuB274
Cucsa.178530	Melon (DHL92) v3.5.1	cgymeB320
Cucsa.178530	Watermelon (Charleston Gray)	cgywcgB319
Cucsa.178530	Watermelon (97103) v1	cgywmB342