Cucsa.177230 (gene) Cucumber (Gy14) v1

Overview
NameCucsa.177230
Typegene
OrganismCucumis sativus (Cucumber (Gy14) v1)
DescriptionWRKY transcription factor
Locationscaffold01225 : 1247154 .. 1248879 (+)
Genome Viewer
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
TTCTTCTAATCCACCAAATTAATCATATCTTTTCTTCTTCTTTCACTTGGCTTTTCACTGCCTATGGCTGCTTTCTCCTCTTGGTTGTTGGATTCTGTTGATACTAAATTAGATCTCAATTCTGTGCCTCTCAGATTCTCTGGTGAACCTCCGGTAAGTTCTGTTCAACTTCTTTAATCCTGATCTCTTTtGTTtCTGTTTTATGAGCTTTCGACTGACTTTTTtttttttttGtAGAAGGAGAGGAATTATATGGATGTCGAGAGGAAGGCTACTGTGAAAGAAGAGGTGGGTGTTTCATGGATTTCAAATTTTTGGTTCTGGTTGATATGTTTTtGAGGATGAGATTGATTTtGTTTTCTGTTTTTTTAAATTTTAGACTGGAGCTTTAATGGAGGAATTGAAGAGGGTTAGTGCAGAGAACAAGAAATTGACTGAAATGTTGACTGTTGTTTGTGAAAATTACAACACTTTGAGAGGACATTTGATGGAACAAATGAACAAAAATGGAGAAAAAGAGATTTCTTCTTCAAAGAAGAGGAAATCAGAGAGTAGCAATAACAATAACAATATGGCTGGAATGAATGGGAATTCTGAAAGTAGTTCAACTGATGAAGAATCTTACAAGAAACCAAAAGAAGAAACCATTAGCAAATCAGCTAAAATCACTAGAGTTCAAGTCAAAATTGGAGCTTCTGATTCAAATCTGGTGAGTTTTAAACCCCAAAATTTCAAATGGCAAAATGGGGGATTCTCCCAATCTAAATTAAACTTACCCATTTCTTTGAATTTAAATTTTGTAGGTTGTTAAAGATGGATATCAATGGAGGAAATATGGTCAAAAAGTCACTAGAGATAATCCTTGTCCTAGAGCTTATTTCAAATGCTCTTTTGCTCCTAGCTGCCCTGTTAAAAAGAAGGTGAACAACAACAACAAACTTTTTCTGtCCTTTTTGACAATTTCCCCTTTCTAAAAAAAAAaGGTTTGTTCTAAATGGAGGATTTAATTTGTAGGTTCAAAGAAGTGTTGAAGATCAATCAGTTCTTGTAGCCACATATGAAGGTGAACACAATCATCCACACCCATCTCAGATTGAGGCCACTTCCGGTGGCGCCGCCGCCCGTAGTGTCAACATAGCTCCGGCAGTGGTTTCGGCAGCGCCGGGATCCTCGTCAGCGCAGGCAGTTTCTCTGGACTTGGTGAAGGCGAAACCGATCGCGATTATGGAAGCTAAAACTTTCGCGAACCCCAAATTTGACTCACCAGAGTTGCAACAGTTTCTCGTCGAGCAGATGGCCTCTTCATTAACGAAAGATCCCAATTTCACGGCGGCGCTTGCGGCGGCGATTTCGGGAAAGATTTTCCCACATTGATTGAAGTTTAGAAGTTGAAGATACAAATCTCTTTTGGAATTAATCTGTAAATACACACTGAAACAGAGAAGATTTGACGGATTTTAGGAAGTGGAAGTTTTGAGATTTTTGAGAAAGGGGGAGAAAACGTTGAAAAAGAGTACTAAAAATGGATGAAAAATGAAAAATATTAGAAATTCAAATTGGGTACTTTGTTGACGTTTTTAGGTCGTCCAACAATTTCAATTTGTAATATGGCAAAATTCTTCAAGCAATTGAATTTGATATCAATAAGAACTCTCTCTCTCCAAAGTCAATACAGTATATGTAACGTTTTGACAAAGGGGCAAGGGGTTTAAATATTTTGCTTACA

mRNA sequence

TTCTTCTAATCCACCAAATTAATCATATCTTTTCTTCTTCTTTCACTTGGCTTTTCACTGCCTATGGCTGCTTTCTCCTCTTGGTTGTTGGATTCTGTTGATACTAAATTAGATCTCAATTCTGTGCCTCTCAGATTCTCTGGTGAACCTCCGAAGGAGAGGAATTATATGGATGTCGAGAGGAAGGCTACTGTGAAAGAAGAGACTGGAGCTTTAATGGAGGAATTGAAGAGGGTTAGTGCAGAGAACAAGAAATTGACTGAAATGTTGACTGTTGTTTGTGAAAATTACAACACTTTGAGAGGACATTTGATGGAACAAATGAACAAAAATGGAGAAAAAGAGATTTCTTCTTCAAAGAAGAGGAAATCAGAGAGTAGCAATAACAATAACAATATGGCTGGAATGAATGGGAATTCTGAAAGTAGTTCAACTGATGAAGAATCTTACAAGAAACCAAAAGAAGAAACCATTAGCAAATCAGCTAAAATCACTAGAGTTCAAGTCAAAATTGGAGCTTCTGATTCAAATCTGGTTGTTAAAGATGGATATCAATGGAGGAAATATGGTCAAAAAGTCACTAGAGATAATCCTTGTCCTAGAGCTTATTTCAAATGCTCTTTTGCTCCTAGCTGCCCTGTTAAAAAGAAGGTTCAAAGAAGTGTTGAAGATCAATCAGTTCTTGTAGCCACATATGAAGGTGAACACAATCATCCACACCCATCTCAGATTGAGGCCACTTCCGGTGGCGCCGCCGCCCGTAGTGTCAACATAGCTCCGGCAGTGGTTTCGGCAGCGCCGGGATCCTCGTCAGCGCAGGCAGTTTCTCTGGACTTGGTGAAGGCGAAACCGATCGCGATTATGGAAGCTAAAACTTTCGCGAACCCCAAATTTGACTCACCAGAGTTGCAACAGTTTCTCGTCGAGCAGATGGCCTCTTCATTAACGAAAGATCCCAATTTCACGGCGGCGCTTGCGGCGGCGATTTCGGGAAAGATTTTCCCACATTGATTGAAGTTTAGAAGTTGAAGATACAAATCTCTTTTGGAATTAATCTGTAAATACACACTGAAACAGAGAAGATTTGACGGATTTTAGGAAGTGGAAGTTTTGAGATTTTTGAGAAAGGGGGAGAAAACGTTGAAAAAGAGTACTAAAAATGGATGAAAAATGAAAAATATTAGAAATTCAAATTGGGTACTTTGTTGACGTTTTTAGGTCGTCCAACAATTTCAATTTGTAATATGGCAAAATTCTTCAAGCAATTGAATTTGATATCAATAAGAACTCTCTCTCTCCAAAGTCAATACAGTATATGTAACGTTTTGACAAAGGGGCAAGGGGTTTAAATATTTTGCTTACA

Coding sequence (CDS)

ATGGCTGCTTTCTCCTCTTGGTTGTTGGATTCTGTTGATACTAAATTAGATCTCAATTCTGTGCCTCTCAGATTCTCTGGTGAACCTCCGAAGGAGAGGAATTATATGGATGTCGAGAGGAAGGCTACTGTGAAAGAAGAGACTGGAGCTTTAATGGAGGAATTGAAGAGGGTTAGTGCAGAGAACAAGAAATTGACTGAAATGTTGACTGTTGTTTGTGAAAATTACAACACTTTGAGAGGACATTTGATGGAACAAATGAACAAAAATGGAGAAAAAGAGATTTCTTCTTCAAAGAAGAGGAAATCAGAGAGTAGCAATAACAATAACAATATGGCTGGAATGAATGGGAATTCTGAAAGTAGTTCAACTGATGAAGAATCTTACAAGAAACCAAAAGAAGAAACCATTAGCAAATCAGCTAAAATCACTAGAGTTCAAGTCAAAATTGGAGCTTCTGATTCAAATCTGGTTGTTAAAGATGGATATCAATGGAGGAAATATGGTCAAAAAGTCACTAGAGATAATCCTTGTCCTAGAGCTTATTTCAAATGCTCTTTTGCTCCTAGCTGCCCTGTTAAAAAGAAGGTTCAAAGAAGTGTTGAAGATCAATCAGTTCTTGTAGCCACATATGAAGGTGAACACAATCATCCACACCCATCTCAGATTGAGGCCACTTCCGGTGGCGCCGCCGCCCGTAGTGTCAACATAGCTCCGGCAGTGGTTTCGGCAGCGCCGGGATCCTCGTCAGCGCAGGCAGTTTCTCTGGACTTGGTGAAGGCGAAACCGATCGCGATTATGGAAGCTAAAACTTTCGCGAACCCCAAATTTGACTCACCAGAGTTGCAACAGTTTCTCGTCGAGCAGATGGCCTCTTCATTAACGAAAGATCCCAATTTCACGGCGGCGCTTGCGGCGGCGATTTCGGGAAAGATTTTCCCACATTGA

Protein sequence

MAAFSSWLLDSVDTKLDLNSVPLRFSGEPPKERNYMDVERKATVKEETGALMEELKRVSAENKKLTEMLTVVCENYNTLRGHLMEQMNKNGEKEISSSKKRKSESSNNNNNMAGMNGNSESSSTDEESYKKPKEETISKSAKITRVQVKIGASDSNLVVKDGYQWRKYGQKVTRDNPCPRAYFKCSFAPSCPVKKKVQRSVEDQSVLVATYEGEHNHPHPSQIEATSGGAAARSVNIAPAVVSAAPGSSSAQAVSLDLVKAKPIAIMEAKTFANPKFDSPELQQFLVEQMASSLTKDPNFTAALAAAISGKIFPH*
Homology
[Close]
BLAST of Cucsa.177230 vs. Swiss-Prot
Match: WRK40_ARATH (Probable WRKY transcription factor 40 OS=Arabidopsis thaliana GN=WRKY40 PE=1 SV=1)

HSP 1 Score: 287.7 bits (735), Expect = 1.5e-76
Identity = 165/311 (53.05%), Postives = 209/311 (67.20%), Query Frame = 1 

Query: 12  VDTKLDLNS--VPLRFSGEPPKERNYMDVERKATVKEETGALMEELKRVSAENKKLTEML 71
           VDT LDL      +R   +PP                 T AL+EEL RVSAENKKL+EML
Sbjct: 9   VDTSLDLTIGVTRMRVEEDPP-----------------TSALVEELNRVSAENKKLSEML 68

Query: 72  TVVCENYNTLRGHLMEQMNKNGEKE---ISSSKKRKSESSNNNNNMAGMNGNSESSSTDE 131
           T++C+NYN LR  LME +NK+   E   IS  KKRKS +  +  + A + G SESSSTD+
Sbjct: 69  TLMCDNYNVLRKQLMEYVNKSNITERDQISPPKKRKSPAREDAFSCAVIGGVSESSSTDQ 128

Query: 132 ESY--KKPKEETISKSAKITRVQVKIGASDSNLVVKDGYQWRKYGQKVTRDNPCPRAYFK 191
           + Y  KK +EET+ K  K++RV  K  ASD+ LVVKDGYQWRKYGQKVTRDNP PRAYFK
Sbjct: 129 DEYLCKKQREETVVKE-KVSRVYYKTEASDTTLVVKDGYQWRKYGQKVTRDNPSPRAYFK 188

Query: 192 CSFAPSCPVKKKVQRSVEDQSVLVATYEGEHNHPHPSQIEATSG--GAAARSVNIAPAVV 251
           C+ APSC VKKKVQRSVEDQSVLVATYEGEHNHP PSQI++ +G     +   + +  V 
Sbjct: 189 CACAPSCSVKKKVQRSVEDQSVLVATYEGEHNHPMPSQIDSNNGLNRHISHGGSASTPVA 248

Query: 252 SAAPGSSSAQAVSLDLVKAKPIAIMEAKTFANPKFDSPELQQFLVEQMASSLTKDPNFTA 311
           +    S +    ++D++++K +      T    + D P++Q+ LVEQMASSLTKDPNFTA
Sbjct: 249 ANRRSSLTVPVTTVDMIESKKV------TSPTSRIDFPQVQKLLVEQMASSLTKDPNFTA 295

Query: 312 ALAAAISGKIF 314
           ALAAA++GK++
Sbjct: 309 ALAAAVTGKLY 295

[Close]
BLAST of Cucsa.177230 vs. Swiss-Prot
Match: WRK18_ARATH (WRKY transcription factor 18 OS=Arabidopsis thaliana GN=WRKY18 PE=1 SV=2)

HSP 1 Score: 224.9 bits (572), Expect = 1.2e-57
Identity = 150/328 (45.73%), Postives = 198/328 (60.37%), Query Frame = 1 

Query: 12  VDTKLDLNSVPLRFSGEPPKERNYM----DVERKATVKEETGA-LMEELKRVSAENKKLT 71
           +D  LDLN+ P  FS + PK+   +     ++RK   ++E+ + L EEL RV++ENKKLT
Sbjct: 7   LDISLDLNTNP--FSAKLPKKEVSVLASTHLKRKWLEQDESASELREELNRVNSENKKLT 66

Query: 72  EMLTVVCENYNTLRGHLMEQMNKNGEKEISSS----KKRKSESSNNNNNMAGMN-GNSES 131
           EML  VCE+YN L  HL E++      EI  +    KKRK +         G++ G +E+
Sbjct: 67  EMLARVCESYNELHNHL-EKLQSRQSPEIEQTDIPIKKRKQDPDEFLGFPIGLSSGKTEN 126

Query: 132 SSTDEE---------------SYKKPKEETISKSAKITRVQVKIGASDSNLVVKDGYQWR 191
           SS++E+               S K+P  ++ +K AK++ V V    SD++L VKDG+QWR
Sbjct: 127 SSSNEDHHHHHQQHEQKNQLLSCKRPVTDSFNK-AKVSTVYVPTETSDTSLTVKDGFQWR 186

Query: 192 KYGQKVTRDNPCPRAYFKCSFAPSCPVKKKVQRSVEDQSVLVATYEGEHNH--PHPSQIE 251
           KYGQKVTRDNP PRAYF+CSFAPSCPVKKKVQRS ED S+LVATYEG HNH  P+ S+ +
Sbjct: 187 KYGQKVTRDNPSPRAYFRCSFAPSCPVKKKVQRSAEDPSLLVATYEGTHNHLGPNASEGD 246

Query: 252 ATSGGAAARSVNIAPAVVSAAPGSSSAQAVSLDLVKAKPIAIMEAKTFANPKFDSPELQQ 311
           ATS G                 GSS+   V+LDLV       +E     N       +Q+
Sbjct: 247 ATSQG-----------------GSST---VTLDLVNGCHRLALEKNERDN------TMQE 304

Query: 312 FLVEQMASSLTKDPNFTAALAAAISGKI 313
            L++QMASSLTKD  FTAALAAAISG++
Sbjct: 307 VLIQQMASSLTKDSKFTAALAAAISGRL 304

[Close]
BLAST of Cucsa.177230 vs. Swiss-Prot
Match: WRK60_ARATH (Probable WRKY transcription factor 60 OS=Arabidopsis thaliana GN=WRKY60 PE=1 SV=1)

HSP 1 Score: 199.5 bits (506), Expect = 5.4e-50
Identity = 133/313 (42.49%), Postives = 167/313 (53.35%), Query Frame = 1 

Query: 12  VDTKLDLNSVPLRFSGEPPKERNYMD----VERKATVKEET-GALMEELKRVSAENKKLT 71
           +D   + N   L FSG+ PK          VE+K  VK+E    L +E+ RV++ENKKLT
Sbjct: 1   MDYDPNTNPFDLHFSGKLPKREVSASASKVVEKKWLVKDEKRNMLQDEINRVNSENKKLT 60

Query: 72  EMLTVVCENYNTLRGHLMEQMNKNGEKEISSSKKRKSESSNN--NNNMAGMNGNS--ESS 131
           EML  VCE Y  L  +LME++           + RKS  S N  N  + G       E  
Sbjct: 61  EMLARVCEKYYALN-NLMEEL-----------QSRKSPESVNFQNKQLTGKRKQELDEFV 120

Query: 132 STDEESYKKPKEETISKSAKITRVQVKIGASDSNLVVKDGYQWRKYGQKVTRDNPCPRAY 191
           S+       P E   +  A ++        SD++L VKDGYQWRKYGQK+TRDNP PRAY
Sbjct: 121 SSPIGLSLGPIENITNDKATVSTAYFAAEKSDTSLTVKDGYQWRKYGQKITRDNPSPRAY 180

Query: 192 FKCSFAPSCPVKKKVQRSVEDQSVLVATYEGEHNHPHPSQIEATSGGAAARSVNIAPAVV 251
           F+CSF+PSC VKKKVQRS ED S LVATYEG HNH                         
Sbjct: 181 FRCSFSPSCLVKKKVQRSAEDPSFLVATYEGTHNH------------------------- 240

Query: 252 SAAPGSSSAQAVSLDLVKAKPIAIMEAKTFANPKFDSPELQQFLVEQMASSLTKDPNFTA 311
              P +S ++ V LDLV+     + E K       +   +Q+ LV+QMASSLTKDP FTA
Sbjct: 241 -TGPHASVSRTVKLDLVQGGLEPVEEKK-------ERGTIQEVLVQQMASSLTKDPKFTA 268

Query: 312 ALAAAISGKIFPH 316
           ALA AISG++  H
Sbjct: 301 ALATAISGRLIEH 268

[Close]
BLAST of Cucsa.177230 vs. Swiss-Prot
Match: WRKY6_ARATH (WRKY transcription factor 6 OS=Arabidopsis thaliana GN=WRKY6 PE=1 SV=1)

HSP 1 Score: 135.2 bits (339), Expect = 1.3e-30
Identity = 85/284 (29.93%), Postives = 139/284 (48.94%), Query Frame = 1 

Query: 13  DTKLDLNS-VPLRFSGEPPKERNYMDVERKATV-----KEETGALMEELKRVSAENKKLT 72
           D + D+N+ + LR +G    + + +D    + +     K E   L +ELK+++ +N+KL 
Sbjct: 116 DDRTDVNTGLNLRTTGNTKSDESMIDDGESSEMEDKRAKNELVKLQDELKKMTMDNQKLR 175

Query: 73  EMLTVVCENYNTLRGH---LMEQMNKNGEKEISSSKKRK--------------------- 132
           E+LT V  +Y +L+ H   LM+Q  +   K I +++K +                     
Sbjct: 176 ELLTQVSNSYTSLQMHLVSLMQQQQQQNNKVIEAAEKPEETIVPRQFIDLGPTRAVGEAE 235

Query: 133 --SESSNNNNNMAGMN------------GNSESSSTDEESYKKPKEET-----ISKSAKI 192
             S SS+ +   +G +            G  ES  T+    +K    T      +  A +
Sbjct: 236 DVSNSSSEDRTRSGGSSAAERRSNGKRLGREESPETESNKIQKVNSTTPTTFDQTAEATM 295

Query: 193 TRVQVKIGASDSNLVVKDGYQWRKYGQKVTRDNPCPRAYFKCSFAPSCPVKKKVQRSVED 248
            + +V + A     ++ DG QWRKYGQK+ + NPCPRAY++C+ A  CPV+K+VQR  ED
Sbjct: 296 RKARVSVRARSEAPMISDGCQWRKYGQKMAKGNPCPRAYYRCTMATGCPVRKQVQRCAED 355


HSP 2 Score: 33.5 bits (75), Expect = 5.1e+00
Identity = 17/30 (56.67%), Postives = 19/30 (63.33%), Query Frame = 1 

Query: 283 QQFLVEQMASSLTKDPNFTAALAAAISGKI 313
           Q   V    ++LT DPNFTAALAA IS  I
Sbjct: 509 QSHAVADTITALTADPNFTAALAAVISSMI 538

[Close]
BLAST of Cucsa.177230 vs. Swiss-Prot
Match: WRK61_ARATH (Probable WRKY transcription factor 61 OS=Arabidopsis thaliana GN=WRKY61 PE=2 SV=1)

HSP 1 Score: 121.7 bits (304), Expect = 1.4e-26
Identity = 63/166 (37.95%), Postives = 92/166 (55.42%), Query Frame = 1 

Query: 88  NKNGEKEISSSKKRKSESSNNNNNMAGMNGNSESSSTDEESYKKPKEETISKSAKITRVQ 147
           N N + EI  +++  S   +NNN +   N     +  D+       E+ I     + + +
Sbjct: 123 NPNEKLEIDHNQETMSLEISNNNKIRSQNSFGFKNDGDDHE----DEDEILPQNLVKKTR 182

Query: 148 VKIGASDSNLVVKDGYQWRKYGQKVTRDNPCPRAYFKCSFAPSCPVKKKVQRSVEDQSVL 207
           V + +      + DG QWRKYGQK+ + NPCPRAY++C+ A SCPV+K+VQR  ED S+L
Sbjct: 183 VSVRSRCETPTMNDGCQWRKYGQKIAKGNPCPRAYYRCTIAASCPVRKQVQRCSEDMSIL 242

Query: 208 VATYEGEHNHPHPSQIEATSGGAAARSVNIAPAVVSAAPGSSSAQA 254
           ++TYEG HNHP P    A +   +A     A  ++S A  SSSA A
Sbjct: 243 ISTYEGTHNHPLPMSATAMASATSA----AASMLLSGASSSSSAAA 280

[Close]
BLAST of Cucsa.177230 vs. TrEMBL
Match: A0A0A0L9M6_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_3G710870 PE=4 SV=1)

HSP 1 Score: 621.3 bits (1601), Expect = 6.4e-175
Identity = 315/315 (100.00%), Postives = 315/315 (100.00%), Query Frame = 1 

Query: 1   MAAFSSWLLDSVDTKLDLNSVPLRFSGEPPKERNYMDVERKATVKEETGALMEELKRVSA 60
           MAAFSSWLLDSVDTKLDLNSVPLRFSGEPPKERNYMDVERKATVKEETGALMEELKRVSA
Sbjct: 1   MAAFSSWLLDSVDTKLDLNSVPLRFSGEPPKERNYMDVERKATVKEETGALMEELKRVSA 60

Query: 61  ENKKLTEMLTVVCENYNTLRGHLMEQMNKNGEKEISSSKKRKSESSNNNNNMAGMNGNSE 120
           ENKKLTEMLTVVCENYNTLRGHLMEQMNKNGEKEISSSKKRKSESSNNNNNMAGMNGNSE
Sbjct: 61  ENKKLTEMLTVVCENYNTLRGHLMEQMNKNGEKEISSSKKRKSESSNNNNNMAGMNGNSE 120

Query: 121 SSSTDEESYKKPKEETISKSAKITRVQVKIGASDSNLVVKDGYQWRKYGQKVTRDNPCPR 180
           SSSTDEESYKKPKEETISKSAKITRVQVKIGASDSNLVVKDGYQWRKYGQKVTRDNPCPR
Sbjct: 121 SSSTDEESYKKPKEETISKSAKITRVQVKIGASDSNLVVKDGYQWRKYGQKVTRDNPCPR 180

Query: 181 AYFKCSFAPSCPVKKKVQRSVEDQSVLVATYEGEHNHPHPSQIEATSGGAAARSVNIAPA 240
           AYFKCSFAPSCPVKKKVQRSVEDQSVLVATYEGEHNHPHPSQIEATSGGAAARSVNIAPA
Sbjct: 181 AYFKCSFAPSCPVKKKVQRSVEDQSVLVATYEGEHNHPHPSQIEATSGGAAARSVNIAPA 240

Query: 241 VVSAAPGSSSAQAVSLDLVKAKPIAIMEAKTFANPKFDSPELQQFLVEQMASSLTKDPNF 300
           VVSAAPGSSSAQAVSLDLVKAKPIAIMEAKTFANPKFDSPELQQFLVEQMASSLTKDPNF
Sbjct: 241 VVSAAPGSSSAQAVSLDLVKAKPIAIMEAKTFANPKFDSPELQQFLVEQMASSLTKDPNF 300

Query: 301 TAALAAAISGKIFPH 316
           TAALAAAISGKIFPH
Sbjct: 301 TAALAAAISGKIFPH 315

[Close]
BLAST of Cucsa.177230 vs. TrEMBL
Match: E7CEW9_CUCSA (WRKY protein OS=Cucumis sativus GN=WRKY21 PE=2 SV=1)

HSP 1 Score: 618.6 bits (1594), Expect = 4.2e-174
Identity = 314/315 (99.68%), Postives = 314/315 (99.68%), Query Frame = 1 

Query: 1   MAAFSSWLLDSVDTKLDLNSVPLRFSGEPPKERNYMDVERKATVKEETGALMEELKRVSA 60
           MAAFSSWLLDSVDTKLD NSVPLRFSGEPPKERNYMDVERKATVKEETGALMEELKRVSA
Sbjct: 1   MAAFSSWLLDSVDTKLDHNSVPLRFSGEPPKERNYMDVERKATVKEETGALMEELKRVSA 60

Query: 61  ENKKLTEMLTVVCENYNTLRGHLMEQMNKNGEKEISSSKKRKSESSNNNNNMAGMNGNSE 120
           ENKKLTEMLTVVCENYNTLRGHLMEQMNKNGEKEISSSKKRKSESSNNNNNMAGMNGNSE
Sbjct: 61  ENKKLTEMLTVVCENYNTLRGHLMEQMNKNGEKEISSSKKRKSESSNNNNNMAGMNGNSE 120

Query: 121 SSSTDEESYKKPKEETISKSAKITRVQVKIGASDSNLVVKDGYQWRKYGQKVTRDNPCPR 180
           SSSTDEESYKKPKEETISKSAKITRVQVKIGASDSNLVVKDGYQWRKYGQKVTRDNPCPR
Sbjct: 121 SSSTDEESYKKPKEETISKSAKITRVQVKIGASDSNLVVKDGYQWRKYGQKVTRDNPCPR 180

Query: 181 AYFKCSFAPSCPVKKKVQRSVEDQSVLVATYEGEHNHPHPSQIEATSGGAAARSVNIAPA 240
           AYFKCSFAPSCPVKKKVQRSVEDQSVLVATYEGEHNHPHPSQIEATSGGAAARSVNIAPA
Sbjct: 181 AYFKCSFAPSCPVKKKVQRSVEDQSVLVATYEGEHNHPHPSQIEATSGGAAARSVNIAPA 240

Query: 241 VVSAAPGSSSAQAVSLDLVKAKPIAIMEAKTFANPKFDSPELQQFLVEQMASSLTKDPNF 300
           VVSAAPGSSSAQAVSLDLVKAKPIAIMEAKTFANPKFDSPELQQFLVEQMASSLTKDPNF
Sbjct: 241 VVSAAPGSSSAQAVSLDLVKAKPIAIMEAKTFANPKFDSPELQQFLVEQMASSLTKDPNF 300

Query: 301 TAALAAAISGKIFPH 316
           TAALAAAISGKIFPH
Sbjct: 301 TAALAAAISGKIFPH 315

[Close]
BLAST of Cucsa.177230 vs. TrEMBL
Match: W9QXZ3_9ROSA (Putative WRKY transcription factor 40 OS=Morus notabilis GN=L484_013515 PE=4 SV=1)

HSP 1 Score: 373.6 bits (958), Expect = 2.3e-100
Identity = 203/309 (65.70%), Postives = 235/309 (76.05%), Query Frame = 1 

Query: 12  VDTKLDLNSVPLRFSGEPPK---ERNYMDVERKATVKEETGALMEELKRVSAENKKLTEM 71
           VDT LDLNS PLR   + P    E   +D + K + KEE GAL+EELKRVSAENKKLTEM
Sbjct: 8   VDTSLDLNSKPLRLMEDAPNKEVESKLIDFQMKLSAKEENGALVEELKRVSAENKKLTEM 67

Query: 72  LTVVCENYNTLRGHLMEQMNKN-GEKEISSSKKRKSESSNN-NNNMAGM-NGNSESSSTD 131
           LTV+CENYNTLR  LME MNK+  EKE+S +KKRKSESSNN + N+ G+ NGNSESSSTD
Sbjct: 68  LTVMCENYNTLRSQLMEYMNKSQAEKELSPTKKRKSESSNNTSTNVVGVNNGNSESSSTD 127

Query: 132 EESYKKPKEETISKSAKITRVQVKIGASDSNLVVKDGYQWRKYGQKVTRDNPCPRAYFKC 191
           +ES+KKP+EE I    KI +   K  ASD++L+VKDGYQWRKYGQKVTRDNP PRAYFKC
Sbjct: 128 DESFKKPREEPI--KTKIWKTYYKTEASDTSLIVKDGYQWRKYGQKVTRDNPSPRAYFKC 187

Query: 192 SFAPSCPVKKKVQRSVEDQSVLVATYEGEHNHPHPSQIEATSGGAAARSVNIAPAVVSAA 251
           SFAPSCPVKKKVQRSVEDQS+LVATYEGEHNHP PSQ+EATSG     ++N  P   S  
Sbjct: 188 SFAPSCPVKKKVQRSVEDQSILVATYEGEHNHPQPSQLEATSGSNRCMTINTVPFSTSIV 247

Query: 252 PGSSSAQAVSLDLVKAKPIAIMEAKTFANP-KFDSPELQQFLVEQMASSLTKDPNFTAAL 311
              SSA  ++LD  K K  +  +    A P + DS E++Q LVEQMASSLTKDPNFTAAL
Sbjct: 248 ---SSAPNITLDFTKPKASSTSDTTKSAKPARVDSQEVRQILVEQMASSLTKDPNFTAAL 307

Query: 312 AAAISGKIF 314
           AAAISG+I+
Sbjct: 308 AAAISGRIY 311

[Close]
BLAST of Cucsa.177230 vs. TrEMBL
Match: A0A140H8T3_MANES (WRKY transcription factor 79 OS=Manihot esculenta PE=2 SV=1)

HSP 1 Score: 372.1 bits (954), Expect = 6.8e-100
Identity = 206/308 (66.88%), Postives = 242/308 (78.57%), Query Frame = 1 

Query: 12  VDTKLDLNSVPLRFSGEP-PKER----NYMDVERKATVKEETGALMEELKRVSAENKKLT 71
           VDT LDLN  P R +     +ER    N+M+  +K +VKEETGAL+EEL RVSAENKKLT
Sbjct: 19  VDTSLDLNINPRRPNDHALVQERLGLANFMEFGKKISVKEETGALVEELNRVSAENKKLT 78

Query: 72  EMLTVVCENYNTLRGHLMEQMNKNGEKEISSSKKRKSESSNNNN--NMAGMNGNSESSST 131
           EMLTV+CENYN L+  LM+ MNKN +KE+S S+KRKSESSNNNN  N   MNGNSESSST
Sbjct: 79  EMLTVMCENYNALKSQLMDYMNKNQDKELSPSRKRKSESSNNNNNDNTIVMNGNSESSST 138

Query: 132 DEESYKKPKEETISKSAKITRVQVKIGASDSNLVVKDGYQWRKYGQKVTRDNPCPRAYFK 191
           DEES KKP+E+ I   AKI+R  V+  A+D++LVVKDGYQWRKYGQKVTRDNP PRAYFK
Sbjct: 139 DEESCKKPREQVI--KAKISRTYVRTEANDTSLVVKDGYQWRKYGQKVTRDNPSPRAYFK 198

Query: 192 CSFAPSCPVKKKVQRSVEDQSVLVATYEGEHNHPHPSQIEATSGGAAARSVNIAPAVVSA 251
           CSFAPSCPVKKKVQRS+EDQSVLVATYEGEHNHPHPSQ+EATS  AA RS+N+     SA
Sbjct: 199 CSFAPSCPVKKKVQRSIEDQSVLVATYEGEHNHPHPSQMEATS--AATRSLNLGSVPCSA 258

Query: 252 APGSSSAQAVSLDLVKAKPIAIMEAKTFANPKFDSPELQQFLVEQMASSLTKDPNFTAAL 311
             G SS   ++LDL K+   +  E ++ +  + ++PE QQ LVEQMASSLTKDPNFTAAL
Sbjct: 259 TLG-SSGPTITLDLTKSNK-SNNEPRS-SKSRVETPEAQQLLVEQMASSLTKDPNFTAAL 318

Query: 312 AAAISGKI 313
           AAAISG++
Sbjct: 319 AAAISGRM 319

[Close]
BLAST of Cucsa.177230 vs. TrEMBL
Match: A9PHD6_POPTR (Putative uncharacterized protein OS=Populus trichocarpa PE=2 SV=1)

HSP 1 Score: 367.5 bits (942), Expect = 1.7e-98
Identity = 199/322 (61.80%), Postives = 240/322 (74.53%), Query Frame = 1 

Query: 4   FSSWLLDSVDTKLDLNSVPLRFSGEPPKERN-----------YMDVERKATVKEETGALM 63
           +SSW    VDT LDLN  PLR   E P +             +MD + K + KEETGAL+
Sbjct: 3   YSSW----VDTSLDLNINPLRVKSEVPVDAERFGMARELKPTFMDFQTKPSAKEETGALV 62

Query: 64  EELKRVSAENKKLTEMLTVVCENYNTLRGHLMEQMNKNGEKEI-SSSKKRKSESSNNNNN 123
            EL RVS EN+KLTEMLTV+CE+YN LR  LM+ M+KNGEKE+ + SKKRKSESSNNN+N
Sbjct: 63  VELNRVSEENRKLTEMLTVMCESYNALRSQLMDYMSKNGEKELLAPSKKRKSESSNNNDN 122

Query: 124 MAGMNGNSESSSTDEESYKKPKEETISKSAKITRVQVKIGASDSNLVVKDGYQWRKYGQK 183
              MNG+SESSSTDEES KKP+EE I    KI++  V+  A D++L+VKDGYQWRKYGQK
Sbjct: 123 NIVMNGHSESSSTDEESSKKPREEVIKD--KISKAYVRTEAGDTSLIVKDGYQWRKYGQK 182

Query: 184 VTRDNPCPRAYFKCSFAPSCPVKKKVQRSVEDQSVLVATYEGEHNHPHPSQIEATSGGAA 243
           VTRDNP PRAYFKCSFAPSCPVKKKVQRS++DQSVLVATYEGEHNHPHPS +EATSG   
Sbjct: 183 VTRDNPSPRAYFKCSFAPSCPVKKKVQRSIDDQSVLVATYEGEHNHPHPS-MEATSGSNR 242

Query: 244 ARSVNIAPAVVSAAPGSSSAQAVSLDLVKAKPIAIMEAKTFANPKFDSPELQQFLVEQMA 303
           + ++  AP + S A   SS   ++LDL K+K          + P+ D+PE+++FLVEQMA
Sbjct: 243 SLTLGPAPCIASLA---SSGPTITLDLTKSKS---RNDDRSSKPRADTPEVRKFLVEQMA 302

Query: 304 SSLTKDPNFTAALAAAISGKIF 314
           SSLTKDPNFTAALA+AISG++F
Sbjct: 303 SSLTKDPNFTAALASAISGRMF 311

[Close]
BLAST of Cucsa.177230 vs. TAIR10
Match: AT1G80840.1 (AT1G80840.1 WRKY DNA-binding protein 40)

HSP 1 Score: 287.7 bits (735), Expect = 8.5e-78
Identity = 165/311 (53.05%), Postives = 209/311 (67.20%), Query Frame = 1 

Query: 12  VDTKLDLNS--VPLRFSGEPPKERNYMDVERKATVKEETGALMEELKRVSAENKKLTEML 71
           VDT LDL      +R   +PP                 T AL+EEL RVSAENKKL+EML
Sbjct: 9   VDTSLDLTIGVTRMRVEEDPP-----------------TSALVEELNRVSAENKKLSEML 68

Query: 72  TVVCENYNTLRGHLMEQMNKNGEKE---ISSSKKRKSESSNNNNNMAGMNGNSESSSTDE 131
           T++C+NYN LR  LME +NK+   E   IS  KKRKS +  +  + A + G SESSSTD+
Sbjct: 69  TLMCDNYNVLRKQLMEYVNKSNITERDQISPPKKRKSPAREDAFSCAVIGGVSESSSTDQ 128

Query: 132 ESY--KKPKEETISKSAKITRVQVKIGASDSNLVVKDGYQWRKYGQKVTRDNPCPRAYFK 191
           + Y  KK +EET+ K  K++RV  K  ASD+ LVVKDGYQWRKYGQKVTRDNP PRAYFK
Sbjct: 129 DEYLCKKQREETVVKE-KVSRVYYKTEASDTTLVVKDGYQWRKYGQKVTRDNPSPRAYFK 188

Query: 192 CSFAPSCPVKKKVQRSVEDQSVLVATYEGEHNHPHPSQIEATSG--GAAARSVNIAPAVV 251
           C+ APSC VKKKVQRSVEDQSVLVATYEGEHNHP PSQI++ +G     +   + +  V 
Sbjct: 189 CACAPSCSVKKKVQRSVEDQSVLVATYEGEHNHPMPSQIDSNNGLNRHISHGGSASTPVA 248

Query: 252 SAAPGSSSAQAVSLDLVKAKPIAIMEAKTFANPKFDSPELQQFLVEQMASSLTKDPNFTA 311
           +    S +    ++D++++K +      T    + D P++Q+ LVEQMASSLTKDPNFTA
Sbjct: 249 ANRRSSLTVPVTTVDMIESKKV------TSPTSRIDFPQVQKLLVEQMASSLTKDPNFTA 295

Query: 312 ALAAAISGKIF 314
           ALAAA++GK++
Sbjct: 309 ALAAAVTGKLY 295

[Close]
BLAST of Cucsa.177230 vs. TAIR10
Match: AT4G31800.1 (AT4G31800.1 WRKY DNA-binding protein 18)

HSP 1 Score: 224.9 bits (572), Expect = 6.8e-59
Identity = 150/328 (45.73%), Postives = 198/328 (60.37%), Query Frame = 1 

Query: 12  VDTKLDLNSVPLRFSGEPPKERNYM----DVERKATVKEETGA-LMEELKRVSAENKKLT 71
           +D  LDLN+ P  FS + PK+   +     ++RK   ++E+ + L EEL RV++ENKKLT
Sbjct: 7   LDISLDLNTNP--FSAKLPKKEVSVLASTHLKRKWLEQDESASELREELNRVNSENKKLT 66

Query: 72  EMLTVVCENYNTLRGHLMEQMNKNGEKEISSS----KKRKSESSNNNNNMAGMN-GNSES 131
           EML  VCE+YN L  HL E++      EI  +    KKRK +         G++ G +E+
Sbjct: 67  EMLARVCESYNELHNHL-EKLQSRQSPEIEQTDIPIKKRKQDPDEFLGFPIGLSSGKTEN 126

Query: 132 SSTDEE---------------SYKKPKEETISKSAKITRVQVKIGASDSNLVVKDGYQWR 191
           SS++E+               S K+P  ++ +K AK++ V V    SD++L VKDG+QWR
Sbjct: 127 SSSNEDHHHHHQQHEQKNQLLSCKRPVTDSFNK-AKVSTVYVPTETSDTSLTVKDGFQWR 186

Query: 192 KYGQKVTRDNPCPRAYFKCSFAPSCPVKKKVQRSVEDQSVLVATYEGEHNH--PHPSQIE 251
           KYGQKVTRDNP PRAYF+CSFAPSCPVKKKVQRS ED S+LVATYEG HNH  P+ S+ +
Sbjct: 187 KYGQKVTRDNPSPRAYFRCSFAPSCPVKKKVQRSAEDPSLLVATYEGTHNHLGPNASEGD 246

Query: 252 ATSGGAAARSVNIAPAVVSAAPGSSSAQAVSLDLVKAKPIAIMEAKTFANPKFDSPELQQ 311
           ATS G                 GSS+   V+LDLV       +E     N       +Q+
Sbjct: 247 ATSQG-----------------GSST---VTLDLVNGCHRLALEKNERDN------TMQE 304

Query: 312 FLVEQMASSLTKDPNFTAALAAAISGKI 313
            L++QMASSLTKD  FTAALAAAISG++
Sbjct: 307 VLIQQMASSLTKDSKFTAALAAAISGRL 304

[Close]
BLAST of Cucsa.177230 vs. TAIR10
Match: AT2G25000.1 (AT2G25000.1 WRKY DNA-binding protein 60)

HSP 1 Score: 199.5 bits (506), Expect = 3.0e-51
Identity = 133/313 (42.49%), Postives = 167/313 (53.35%), Query Frame = 1 

Query: 12  VDTKLDLNSVPLRFSGEPPKERNYMD----VERKATVKEET-GALMEELKRVSAENKKLT 71
           +D   + N   L FSG+ PK          VE+K  VK+E    L +E+ RV++ENKKLT
Sbjct: 1   MDYDPNTNPFDLHFSGKLPKREVSASASKVVEKKWLVKDEKRNMLQDEINRVNSENKKLT 60

Query: 72  EMLTVVCENYNTLRGHLMEQMNKNGEKEISSSKKRKSESSNN--NNNMAGMNGNS--ESS 131
           EML  VCE Y  L  +LME++           + RKS  S N  N  + G       E  
Sbjct: 61  EMLARVCEKYYALN-NLMEEL-----------QSRKSPESVNFQNKQLTGKRKQELDEFV 120

Query: 132 STDEESYKKPKEETISKSAKITRVQVKIGASDSNLVVKDGYQWRKYGQKVTRDNPCPRAY 191
           S+       P E   +  A ++        SD++L VKDGYQWRKYGQK+TRDNP PRAY
Sbjct: 121 SSPIGLSLGPIENITNDKATVSTAYFAAEKSDTSLTVKDGYQWRKYGQKITRDNPSPRAY 180

Query: 192 FKCSFAPSCPVKKKVQRSVEDQSVLVATYEGEHNHPHPSQIEATSGGAAARSVNIAPAVV 251
           F+CSF+PSC VKKKVQRS ED S LVATYEG HNH                         
Sbjct: 181 FRCSFSPSCLVKKKVQRSAEDPSFLVATYEGTHNH------------------------- 240

Query: 252 SAAPGSSSAQAVSLDLVKAKPIAIMEAKTFANPKFDSPELQQFLVEQMASSLTKDPNFTA 311
              P +S ++ V LDLV+     + E K       +   +Q+ LV+QMASSLTKDP FTA
Sbjct: 241 -TGPHASVSRTVKLDLVQGGLEPVEEKK-------ERGTIQEVLVQQMASSLTKDPKFTA 268

Query: 312 ALAAAISGKIFPH 316
           ALA AISG++  H
Sbjct: 301 ALATAISGRLIEH 268

[Close]
BLAST of Cucsa.177230 vs. TAIR10
Match: AT1G62300.1 (AT1G62300.1 WRKY family transcription factor)

HSP 1 Score: 135.2 bits (339), Expect = 7.1e-32
Identity = 85/284 (29.93%), Postives = 139/284 (48.94%), Query Frame = 1 

Query: 13  DTKLDLNS-VPLRFSGEPPKERNYMDVERKATV-----KEETGALMEELKRVSAENKKLT 72
           D + D+N+ + LR +G    + + +D    + +     K E   L +ELK+++ +N+KL 
Sbjct: 116 DDRTDVNTGLNLRTTGNTKSDESMIDDGESSEMEDKRAKNELVKLQDELKKMTMDNQKLR 175

Query: 73  EMLTVVCENYNTLRGH---LMEQMNKNGEKEISSSKKRK--------------------- 132
           E+LT V  +Y +L+ H   LM+Q  +   K I +++K +                     
Sbjct: 176 ELLTQVSNSYTSLQMHLVSLMQQQQQQNNKVIEAAEKPEETIVPRQFIDLGPTRAVGEAE 235

Query: 133 --SESSNNNNNMAGMN------------GNSESSSTDEESYKKPKEET-----ISKSAKI 192
             S SS+ +   +G +            G  ES  T+    +K    T      +  A +
Sbjct: 236 DVSNSSSEDRTRSGGSSAAERRSNGKRLGREESPETESNKIQKVNSTTPTTFDQTAEATM 295

Query: 193 TRVQVKIGASDSNLVVKDGYQWRKYGQKVTRDNPCPRAYFKCSFAPSCPVKKKVQRSVED 248
            + +V + A     ++ DG QWRKYGQK+ + NPCPRAY++C+ A  CPV+K+VQR  ED
Sbjct: 296 RKARVSVRARSEAPMISDGCQWRKYGQKMAKGNPCPRAYYRCTMATGCPVRKQVQRCAED 355


HSP 2 Score: 33.5 bits (75), Expect = 2.9e-01
Identity = 17/30 (56.67%), Postives = 19/30 (63.33%), Query Frame = 1 

Query: 283 QQFLVEQMASSLTKDPNFTAALAAAISGKI 313
           Q   V    ++LT DPNFTAALAA IS  I
Sbjct: 509 QSHAVADTITALTADPNFTAALAAVISSMI 538

[Close]
BLAST of Cucsa.177230 vs. TAIR10
Match: AT1G18860.1 (AT1G18860.1 WRKY DNA-binding protein 61)

HSP 1 Score: 121.7 bits (304), Expect = 8.1e-28
Identity = 63/166 (37.95%), Postives = 92/166 (55.42%), Query Frame = 1 

Query: 88  NKNGEKEISSSKKRKSESSNNNNNMAGMNGNSESSSTDEESYKKPKEETISKSAKITRVQ 147
           N N + EI  +++  S   +NNN +   N     +  D+       E+ I     + + +
Sbjct: 123 NPNEKLEIDHNQETMSLEISNNNKIRSQNSFGFKNDGDDHE----DEDEILPQNLVKKTR 182

Query: 148 VKIGASDSNLVVKDGYQWRKYGQKVTRDNPCPRAYFKCSFAPSCPVKKKVQRSVEDQSVL 207
           V + +      + DG QWRKYGQK+ + NPCPRAY++C+ A SCPV+K+VQR  ED S+L
Sbjct: 183 VSVRSRCETPTMNDGCQWRKYGQKIAKGNPCPRAYYRCTIAASCPVRKQVQRCSEDMSIL 242

Query: 208 VATYEGEHNHPHPSQIEATSGGAAARSVNIAPAVVSAAPGSSSAQA 254
           ++TYEG HNHP P    A +   +A     A  ++S A  SSSA A
Sbjct: 243 ISTYEGTHNHPLPMSATAMASATSA----AASMLLSGASSSSSAAA 280

[Close]
BLAST of Cucsa.177230 vs. NCBI nr
Match: gi|700203527|gb|KGN58660.1| (hypothetical protein Csa_3G710870 [Cucumis sativus])

HSP 1 Score: 621.3 bits (1601), Expect = 9.2e-175
Identity = 315/315 (100.00%), Postives = 315/315 (100.00%), Query Frame = 1 

Query: 1   MAAFSSWLLDSVDTKLDLNSVPLRFSGEPPKERNYMDVERKATVKEETGALMEELKRVSA 60
           MAAFSSWLLDSVDTKLDLNSVPLRFSGEPPKERNYMDVERKATVKEETGALMEELKRVSA
Sbjct: 1   MAAFSSWLLDSVDTKLDLNSVPLRFSGEPPKERNYMDVERKATVKEETGALMEELKRVSA 60

Query: 61  ENKKLTEMLTVVCENYNTLRGHLMEQMNKNGEKEISSSKKRKSESSNNNNNMAGMNGNSE 120
           ENKKLTEMLTVVCENYNTLRGHLMEQMNKNGEKEISSSKKRKSESSNNNNNMAGMNGNSE
Sbjct: 61  ENKKLTEMLTVVCENYNTLRGHLMEQMNKNGEKEISSSKKRKSESSNNNNNMAGMNGNSE 120

Query: 121 SSSTDEESYKKPKEETISKSAKITRVQVKIGASDSNLVVKDGYQWRKYGQKVTRDNPCPR 180
           SSSTDEESYKKPKEETISKSAKITRVQVKIGASDSNLVVKDGYQWRKYGQKVTRDNPCPR
Sbjct: 121 SSSTDEESYKKPKEETISKSAKITRVQVKIGASDSNLVVKDGYQWRKYGQKVTRDNPCPR 180

Query: 181 AYFKCSFAPSCPVKKKVQRSVEDQSVLVATYEGEHNHPHPSQIEATSGGAAARSVNIAPA 240
           AYFKCSFAPSCPVKKKVQRSVEDQSVLVATYEGEHNHPHPSQIEATSGGAAARSVNIAPA
Sbjct: 181 AYFKCSFAPSCPVKKKVQRSVEDQSVLVATYEGEHNHPHPSQIEATSGGAAARSVNIAPA 240

Query: 241 VVSAAPGSSSAQAVSLDLVKAKPIAIMEAKTFANPKFDSPELQQFLVEQMASSLTKDPNF 300
           VVSAAPGSSSAQAVSLDLVKAKPIAIMEAKTFANPKFDSPELQQFLVEQMASSLTKDPNF
Sbjct: 241 VVSAAPGSSSAQAVSLDLVKAKPIAIMEAKTFANPKFDSPELQQFLVEQMASSLTKDPNF 300

Query: 301 TAALAAAISGKIFPH 316
           TAALAAAISGKIFPH
Sbjct: 301 TAALAAAISGKIFPH 315

[Close]
BLAST of Cucsa.177230 vs. NCBI nr
Match: gi|793420808|ref|NP_001292634.1| (probable WRKY transcription factor 40 [Cucumis sativus])

HSP 1 Score: 618.6 bits (1594), Expect = 6.0e-174
Identity = 314/315 (99.68%), Postives = 314/315 (99.68%), Query Frame = 1 

Query: 1   MAAFSSWLLDSVDTKLDLNSVPLRFSGEPPKERNYMDVERKATVKEETGALMEELKRVSA 60
           MAAFSSWLLDSVDTKLD NSVPLRFSGEPPKERNYMDVERKATVKEETGALMEELKRVSA
Sbjct: 1   MAAFSSWLLDSVDTKLDHNSVPLRFSGEPPKERNYMDVERKATVKEETGALMEELKRVSA 60

Query: 61  ENKKLTEMLTVVCENYNTLRGHLMEQMNKNGEKEISSSKKRKSESSNNNNNMAGMNGNSE 120
           ENKKLTEMLTVVCENYNTLRGHLMEQMNKNGEKEISSSKKRKSESSNNNNNMAGMNGNSE
Sbjct: 61  ENKKLTEMLTVVCENYNTLRGHLMEQMNKNGEKEISSSKKRKSESSNNNNNMAGMNGNSE 120

Query: 121 SSSTDEESYKKPKEETISKSAKITRVQVKIGASDSNLVVKDGYQWRKYGQKVTRDNPCPR 180
           SSSTDEESYKKPKEETISKSAKITRVQVKIGASDSNLVVKDGYQWRKYGQKVTRDNPCPR
Sbjct: 121 SSSTDEESYKKPKEETISKSAKITRVQVKIGASDSNLVVKDGYQWRKYGQKVTRDNPCPR 180

Query: 181 AYFKCSFAPSCPVKKKVQRSVEDQSVLVATYEGEHNHPHPSQIEATSGGAAARSVNIAPA 240
           AYFKCSFAPSCPVKKKVQRSVEDQSVLVATYEGEHNHPHPSQIEATSGGAAARSVNIAPA
Sbjct: 181 AYFKCSFAPSCPVKKKVQRSVEDQSVLVATYEGEHNHPHPSQIEATSGGAAARSVNIAPA 240

Query: 241 VVSAAPGSSSAQAVSLDLVKAKPIAIMEAKTFANPKFDSPELQQFLVEQMASSLTKDPNF 300
           VVSAAPGSSSAQAVSLDLVKAKPIAIMEAKTFANPKFDSPELQQFLVEQMASSLTKDPNF
Sbjct: 241 VVSAAPGSSSAQAVSLDLVKAKPIAIMEAKTFANPKFDSPELQQFLVEQMASSLTKDPNF 300

Query: 301 TAALAAAISGKIFPH 316
           TAALAAAISGKIFPH
Sbjct: 301 TAALAAAISGKIFPH 315

[Close]
BLAST of Cucsa.177230 vs. NCBI nr
Match: gi|659130403|ref|XP_008465152.1| (PREDICTED: probable WRKY transcription factor 40 [Cucumis melo])

HSP 1 Score: 603.2 bits (1554), Expect = 2.6e-169
Identity = 306/315 (97.14%), Postives = 310/315 (98.41%), Query Frame = 1 

Query: 1   MAAFSSWLLDSVDTKLDLNSVPLRFSGEPPKERNYMDVERKATVKEETGALMEELKRVSA 60
           MAAFSSWLLDSVDTKLDLNS+PLRFSGEPPKERNYMDVERK TVKEETGALMEELKRVSA
Sbjct: 1   MAAFSSWLLDSVDTKLDLNSMPLRFSGEPPKERNYMDVERKVTVKEETGALMEELKRVSA 60

Query: 61  ENKKLTEMLTVVCENYNTLRGHLMEQMNKNGEKEISSSKKRKSESSNNNNNMAGMNGNSE 120
           ENKKLTEMLTVVCENYNTLRGHLMEQMNKNGEKEISSSKKRKSESSNNNNNM GMNGNSE
Sbjct: 61  ENKKLTEMLTVVCENYNTLRGHLMEQMNKNGEKEISSSKKRKSESSNNNNNMVGMNGNSE 120

Query: 121 SSSTDEESYKKPKEETISKSAKITRVQVKIGASDSNLVVKDGYQWRKYGQKVTRDNPCPR 180
           SSSTDEESYKKPKEETI+KSAKITRVQVKIGASDSNLVVKDGYQWRKYGQKVTRDNPCPR
Sbjct: 121 SSSTDEESYKKPKEETINKSAKITRVQVKIGASDSNLVVKDGYQWRKYGQKVTRDNPCPR 180

Query: 181 AYFKCSFAPSCPVKKKVQRSVEDQSVLVATYEGEHNHPHPSQIEATSGGAAARSVNIAPA 240
           AYFKCSFAPSCPVKKKVQRSVEDQSVLVATYEGEHNHPHPSQIEATSGGAAARSVNIAPA
Sbjct: 181 AYFKCSFAPSCPVKKKVQRSVEDQSVLVATYEGEHNHPHPSQIEATSGGAAARSVNIAPA 240

Query: 241 VVSAAPGSSSAQAVSLDLVKAKPIAIMEAKTFANPKFDSPELQQFLVEQMASSLTKDPNF 300
           VV+AAPGSSSAQAVSLDLVKAKP  IMEAKT ANP+FDSPELQQFLVEQMASSLTKDPNF
Sbjct: 241 VVTAAPGSSSAQAVSLDLVKAKP--IMEAKTIANPRFDSPELQQFLVEQMASSLTKDPNF 300

Query: 301 TAALAAAISGKIFPH 316
           TAALAAAISGKIFPH
Sbjct: 301 TAALAAAISGKIFPH 313

[Close]
BLAST of Cucsa.177230 vs. NCBI nr
Match: gi|1009176827|ref|XP_015869638.1| (PREDICTED: probable WRKY transcription factor 40 [Ziziphus jujuba])

HSP 1 Score: 377.5 bits (968), Expect = 2.3e-101
Identity = 205/317 (64.67%), Postives = 238/317 (75.08%), Query Frame = 1 

Query: 4   FSSWLLDSVDTKLDLNSVPLRFSGE---PPKERNYMDVERKATVKEETGALMEELKRVSA 63
           FSS+    VDT LDLN+ PLR   +      +  ++D E+K + +EE+GAL+EEL RV+A
Sbjct: 3   FSSY----VDTSLDLNAKPLRLLDDNLNKEVQSKFIDFEKKLSAREESGALVEELNRVNA 62

Query: 64  ENKKLTEMLTVVCENYNTLRGHLMEQMNKN-GEKEISSSKKRKSESSNNN---NNMAGMN 123
           ENKKLTE LTV+CENYN LR  LME M+KN  EKE++ S+KRKSESSNNN   N +A  N
Sbjct: 63  ENKKLTETLTVMCENYNVLRSQLMEYMSKNLAEKELTPSRKRKSESSNNNSTANILAMNN 122

Query: 124 GNSESSSTDEESYKKPKEETISKSAKITRVQVKIGASDSNLVVKDGYQWRKYGQKVTRDN 183
           G SESSSTDEES KKPKEE     AKI+R  VK  ASDS+L+VKDGYQWRKYGQKVTRDN
Sbjct: 123 GTSESSSTDEESCKKPKEEPTK--AKISRAYVKTEASDSSLIVKDGYQWRKYGQKVTRDN 182

Query: 184 PCPRAYFKCSFAPSCPVKKKVQRSVEDQSVLVATYEGEHNHPHPSQIEATSGGAAARSVN 243
           PCPRAYFKCSFAPSCPVKKKVQRSVEDQS+LVATYEGEHNHPHPSQIEATSG     +  
Sbjct: 183 PCPRAYFKCSFAPSCPVKKKVQRSVEDQSILVATYEGEHNHPHPSQIEATSGSNRCMTTT 242

Query: 244 IAPAVVSAAPGSSSAQAVSLDLVKAKPIAIMEAKTFANPKFDSPELQQFLVEQMASSLTK 303
           +     S   G+S    ++LD  K K  A +     A P+ DSPE++ FLV+QMASSLTK
Sbjct: 243 LGSVPCSTTLGASGPN-ITLDFTKPKSSANVIDTKSAKPRMDSPEVRHFLVDQMASSLTK 302

Query: 304 DPNFTAALAAAISGKIF 314
           DPNFTAALAAAISGKI+
Sbjct: 303 DPNFTAALAAAISGKIY 312

[Close]
BLAST of Cucsa.177230 vs. NCBI nr
Match: gi|703083376|ref|XP_010092197.1| (putative WRKY transcription factor 40 [Morus notabilis])

HSP 1 Score: 373.6 bits (958), Expect = 3.3e-100
Identity = 203/309 (65.70%), Postives = 235/309 (76.05%), Query Frame = 1 

Query: 12  VDTKLDLNSVPLRFSGEPPK---ERNYMDVERKATVKEETGALMEELKRVSAENKKLTEM 71
           VDT LDLNS PLR   + P    E   +D + K + KEE GAL+EELKRVSAENKKLTEM
Sbjct: 8   VDTSLDLNSKPLRLMEDAPNKEVESKLIDFQMKLSAKEENGALVEELKRVSAENKKLTEM 67

Query: 72  LTVVCENYNTLRGHLMEQMNKN-GEKEISSSKKRKSESSNN-NNNMAGM-NGNSESSSTD 131
           LTV+CENYNTLR  LME MNK+  EKE+S +KKRKSESSNN + N+ G+ NGNSESSSTD
Sbjct: 68  LTVMCENYNTLRSQLMEYMNKSQAEKELSPTKKRKSESSNNTSTNVVGVNNGNSESSSTD 127

Query: 132 EESYKKPKEETISKSAKITRVQVKIGASDSNLVVKDGYQWRKYGQKVTRDNPCPRAYFKC 191
           +ES+KKP+EE I    KI +   K  ASD++L+VKDGYQWRKYGQKVTRDNP PRAYFKC
Sbjct: 128 DESFKKPREEPI--KTKIWKTYYKTEASDTSLIVKDGYQWRKYGQKVTRDNPSPRAYFKC 187

Query: 192 SFAPSCPVKKKVQRSVEDQSVLVATYEGEHNHPHPSQIEATSGGAAARSVNIAPAVVSAA 251
           SFAPSCPVKKKVQRSVEDQS+LVATYEGEHNHP PSQ+EATSG     ++N  P   S  
Sbjct: 188 SFAPSCPVKKKVQRSVEDQSILVATYEGEHNHPQPSQLEATSGSNRCMTINTVPFSTSIV 247

Query: 252 PGSSSAQAVSLDLVKAKPIAIMEAKTFANP-KFDSPELQQFLVEQMASSLTKDPNFTAAL 311
              SSA  ++LD  K K  +  +    A P + DS E++Q LVEQMASSLTKDPNFTAAL
Sbjct: 248 ---SSAPNITLDFTKPKASSTSDTTKSAKPARVDSQEVRQILVEQMASSLTKDPNFTAAL 307

Query: 312 AAAISGKIF 314
           AAAISG+I+
Sbjct: 308 AAAISGRIY 311

The following BLAST results are available for this feature:
BLAST of Cucsa.177230 vs. Swiss-Prot
Match NameE-valueIdentityDescription
WRK40_ARATH1.5e-7653.05Probable WRKY transcription factor 40 OS=Arabidopsis thaliana GN=WRKY40 PE=1 SV=... [more]
WRK18_ARATH1.2e-5745.73WRKY transcription factor 18 OS=Arabidopsis thaliana GN=WRKY18 PE=1 SV=2[more]
WRK60_ARATH5.4e-5042.49Probable WRKY transcription factor 60 OS=Arabidopsis thaliana GN=WRKY60 PE=1 SV=... [more]
WRKY6_ARATH1.3e-3029.93WRKY transcription factor 6 OS=Arabidopsis thaliana GN=WRKY6 PE=1 SV=1[more]
WRK61_ARATH1.4e-2637.95Probable WRKY transcription factor 61 OS=Arabidopsis thaliana GN=WRKY61 PE=2 SV=... [more]
BLAST of Cucsa.177230 vs. TrEMBL
Match NameE-valueIdentityDescription
A0A0A0L9M6_CUCSA6.4e-175100.00Uncharacterized protein OS=Cucumis sativus GN=Csa_3G710870 PE=4 SV=1[more]
E7CEW9_CUCSA4.2e-17499.68WRKY protein OS=Cucumis sativus GN=WRKY21 PE=2 SV=1[more]
W9QXZ3_9ROSA2.3e-10065.70Putative WRKY transcription factor 40 OS=Morus notabilis GN=L484_013515 PE=4 SV=... [more]
A0A140H8T3_MANES6.8e-10066.88WRKY transcription factor 79 OS=Manihot esculenta PE=2 SV=1[more]
A9PHD6_POPTR1.7e-9861.80Putative uncharacterized protein OS=Populus trichocarpa PE=2 SV=1[more]
BLAST of Cucsa.177230 vs. TAIR10
Match NameE-valueIdentityDescription
AT1G80840.18.5e-7853.05 WRKY DNA-binding protein 40[more]
AT4G31800.16.8e-5945.73 WRKY DNA-binding protein 18[more]
AT2G25000.13.0e-5142.49 WRKY DNA-binding protein 60[more]
AT1G62300.17.1e-3229.93 WRKY family transcription factor[more]
AT1G18860.18.1e-2837.95 WRKY DNA-binding protein 61[more]
BLAST of Cucsa.177230 vs. NCBI nr
Match NameE-valueIdentityDescription
gi|700203527|gb|KGN58660.1|9.2e-175100.00hypothetical protein Csa_3G710870 [Cucumis sativus][more]
gi|793420808|ref|NP_001292634.1|6.0e-17499.68probable WRKY transcription factor 40 [Cucumis sativus][more]
gi|659130403|ref|XP_008465152.1|2.6e-16997.14PREDICTED: probable WRKY transcription factor 40 [Cucumis melo][more]
gi|1009176827|ref|XP_015869638.1|2.3e-10164.67PREDICTED: probable WRKY transcription factor 40 [Ziziphus jujuba][more]
gi|703083376|ref|XP_010092197.1|3.3e-10065.70putative WRKY transcription factor 40 [Morus notabilis][more]
Annotated Terms
The following terms have been associated with this gene:
Vocabulary: INTERPRO
TermDefinition
IPR003657WRKY_dom
IPR003657WRKY_dom
Vocabulary: Molecular Function
TermDefinition
GO:0003700transcription factor activity, sequence-specific DNA binding
GO:0043565sequence-specific DNA binding
GO:0003700transcription factor activity, sequence-specific DNA binding
GO:0043565sequence-specific DNA binding
Vocabulary: Biological Process
TermDefinition
GO:0006355regulation of transcription, DNA-templated
GO:0006355regulation of transcription, DNA-templated
GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0045892 negative regulation of transcription, DNA-templated
biological_process GO:0031347 regulation of defense response
biological_process GO:0006355 regulation of transcription, DNA-templated
biological_process GO:0002237 response to molecule of bacterial origin
biological_process GO:0050896 response to stimulus
cellular_component GO:0005634 nucleus
cellular_component GO:0005667 transcription factor complex
molecular_function GO:0043565 sequence-specific DNA binding
molecular_function GO:0003700 transcription factor activity, sequence-specific DNA binding
molecular_function GO:0044212 transcription regulatory region DNA binding
Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Cucsa.177230.1Cucsa.177230.1mRNA
Cucsa.177230.2Cucsa.177230.2mRNA


InterPro
Analysis Name: InterPro Annotations of cucumber (Gy14)
Date Performed: 2017-01-17
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR003657WRKY domainGENE3DG3DSA:2.20.25.80coord: 100..169
score: 2.9
IPR003657WRKY domainPFAMPF03106WRKYcoord: 109..167
score: 1.3
IPR003657WRKY domainSMARTSM00774WRKY_clscoord: 108..168
score: 1.1
IPR003657WRKY domainPROFILEPS50811WRKYcoord: 103..169
score: 26
IPR003657WRKY domainunknownSSF118290WRKY DNA-binding domaincoord: 106..169
score: 1.23
NoneNo IPR availablePANTHERPTHR31429FAMILY NOT NAMEDcoord: 2..262
score: 3.6E