Cucsa.175350 (gene) Cucumber (Gy14) v1

NameCucsa.175350
Typegene
OrganismCucumis sativus (Cucumber (Gy14) v1)
DescriptionADP-ribosylation factor GTPase-activating protein
Locationscaffold01225 : 69768 .. 72629 (+)
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
AAAAGGAGGAGCTTTTGACGATTTGTGGAGGGTGTCGGTGTCGCGGCGAAAGAGGAAGAGGTTGAAGCAACCAAAGAAGAAACTGATTTGAAATTTAAGGTTGCGCCCTAATCGGCGGCCCTTTTCCCTTCATAATCAATCCCACCTCTTTCTTTCTGTTTTAACATTATTATTATTATTCAACAAGTTTTGATTTCGATTTTTACATTTTTTCTGAGTTTGGATTTTCTTCCGATTTtACCTTTTTAAATTTGTTCAATACCCAATTGGATCTGATCTTAATGGCGGCGTCACGGCGGCTACGGGACCTTCAATCACGGCCGGGGAATAAGATCTGTGTTGATTGTTCCCAAAAGAATCCCCAATGGGCTTCTGTTTCTTATGGGGTTTTCATGTGTTTGGAATGTTCCGGGAAGCATCGTGGACTTGGTGTTCATATCTCTTTTGTTAGATCTGTTACTATGGATTCTTGGTCTGAGATCCAGATCAAGAAGATGGAGGCTGGAGGTAATGAGCAGCTTAATGCTTTCCTTTCCCAGTATGGAATACCTAAGGAGACTGATATTGTAACCAAATACAATACCAGTGCGGCTGGGGTTTATAGGGATCGGATTCAAGCTCTTGCTGAGGGTCGATCTTGGAAGGATCCACCTGCGGTGAAGGAGAATATTGGAGTAGGAAAATCCAGGCCTCCATTAGCGCAAAGTGCTGGTGGAGGAGGGAGTAAGGTGAATAATGGAGGTTGGGATAGTTGGGATAACGAAGATAATTTTAGATCTTCTAGTGATATGAGGCGTAACCAATCAACAGGCGATGTTCGAGGTATGGGCGGCGGAGGGATGCCGTCTAGGTCTAGATCGACTGAGGATATTTATACACGGTCTCAATTGGAGGCTTCTGCTGCAAATAAAGATAATTTTTTtGCCCAGAAAATTGCTGAGAATGATTCAAGGCCTGAAGGAATTCCGCCATCACAAGGTGGAAAGTATGTTGGATTTGGGTCGAGTCCTGCACCTGCTCGAAGGAACGATCCACAAAATGATGTTTTCTCTGTTGTTTCTCAGGTATTATGTTGTTGTGTGATCAATATTTTCTGCTTGGTTTTGTGTTTATGTTGTGGATTTTCATTTAAGGTTGATCAATGAATGCATCGTTTCATTTCAGGGGTTTGGTAAGTTATCTCTGGTTGCTGCTTCTGCTGCTCAATCGGCTGCAAATGCTGTTCAAGCTGGCACAAAAGAGCTGACTACCAAGGTACTTTCTTTCAGATTGGTGTTAGCACTTTCATGTCGTGATTACTTCCATTGAAATAGATGATATTGCAAATTACAAGAGTTGTATCGTTGGTAGGGTACTTCATATTGAATATGTCATCTCCTGCTTTTGAAAAGATATTATCCATGTTTATATCTTACACTACAAAAAaTGGAGATGACCGTTGTTATATGTGTGAAAATGACTTAGTTTTGGTATTTGCCTAGTTTGCCTTTTCTTTTCCTTGAAAATCCTTCAATTACATCTTCGTGACTACTAGTACTTTGCTTCTGTTTGGTATGCACATTTTTTCTTGTATCTTATAGGCAGCAATGATTTGTTACGGGATCCCTATGGGAGCTAAGTTCTGTTTGGTACTGAAGTTATTATTCTCCCAAATGTACATTTGTAAATTCTTTTTACAGGTGAAGGAGGGTGGCTATGATTACAAAGTAAATGAAACCGTCAATGTTGTCACAGCAAAAACAACAGAAATTGGGCAAAGGACATGGGGAATTATGAGAGGTGTAATGGCAATGGCTTCACAGAAGGTTGAAGAGTATGCCAAAGATGGCATGAACTGGAAGAATGATGGATGGCAACGGAACGAGAATGAGAAAAATGGATATTATCAAGAATTTGAGCATGATAACAAAGGATGGAATTCTTCTTCTGGAACAGGTCAGTCATCAGGAAGTGGACATCACAATAATTCTTACAACTCAAGTTCTTGGGATGATTGGGATACCAAAGACAACAGGAAAGAGGAAACAACAACCAAAGTGAGTGGAACCCATAATAATAACAATAACAACAACAACAATAATAATAGCAATGATGGCTGGGCTGGCTGGGATGACCAAAAAGATGATGGATATGATCACTATTATCAAGCATCCGATCGGAAAACCGTTGGTCAAAATGGAAAAGCAGGTGGTGGTACATGGTCAGAGGGAGGCTTTCTCTGAGGTTTGTAACAATGAACCTAATTCTCAATGGTTGTCTGGATCTTCTTGGATCCTCTCCATATCTCCATGGGAGTAATGATACTTACATGGTTTTATTGTCATAAAGACCTTATAAATATACCCTCTAGAGTCGAAACTCATTTCTTCTTTGGCCACATGACCTGTAATTTTTGTTTATTTTGCATACTTGGCTACGTATGAAAGAAACAATTGTATTCCTGAATCCATTATCCTTACATCTTTATCCCAAGTAATAAAAAGATGGTTGTATGATTAGGTAGCATCTGAAATACACACCACAAGGTAGTTCCTGGTTTCCTCAAATGATGAAAATTTGAATTACTTGGAGTAGCTCATACAGATGGCTGGTCAACACATTGGTAAAGCTAAAGATGATGATTACAGGAACATATAGAATAGAGCTGATAAGATTCTTATGGAAGTGGATATCAAATCCCACTCATGTTTGTTTCAGGTCAGTACATCTTCAGTTTTCACTTTTTCTTTTTtCTTTTTTtCATTTTTTTtGGGAGGGGGgTGGGGGATCATGTTGTGTTTGCAAGTGCTGTATCATCAAAAAAaCGTCGTCCATAGAGGTAAACCCGAATTATAGACACTGTTTATTATGTGGTTGGGAACA

mRNA sequence

AAAAGGAGGAGCTTTTGACGATTTGTGGAGGGTGTCGGTGTCGCGGCGAAAGAGGAAGAGGTTGAAGCAACCAAAGAAGAAACTGATTTGAAATTTAAGGTTGCGCCCTAATCGGCGGCCCTTTTCCCTTCATAATCAATCCCACCTCTTTCTTTCTGTTTTAACATTATTATTATTATTCAACAAGTTTTGATTTCGATTTTTACATTTTTTCTGAGTTTGGATTTTCTTCCGATTTTACCTTTTTAAATTTGTTCAATACCCAATTGGATCTGATCTTAATGGCGGCGTCACGGCGGCTACGGGACCTTCAATCACGGCCGGGGAATAAGATCTGTGTTGATTGTTCCCAAAAGAATCCCCAATGGGCTTCTGTTTCTTATGGGGTTTTCATGTGTTTGGAATGTTCCGGGAAGCATCGTGGACTTGGTGTTCATATCTCTTTTGTTAGATCTGTTACTATGGATTCTTGGTCTGAGATCCAGATCAAGAAGATGGAGGCTGGAGGTAATGAGCAGCTTAATGCTTTCCTTTCCCAGTATGGAATACCTAAGGAGACTGATATTGTAACCAAATACAATACCAGTGCGGCTGGGGTTTATAGGGATCGGATTCAAGCTCTTGCTGAGGGTCGATCTTGGAAGGATCCACCTGCGGTGAAGGAGAATATTGGAGTAGGAAAATCCAGGCCTCCATTAGCGCAAAGTGCTGGTGGAGGAGGGAGTAAGGTGAATAATGGAGGTTGGGATAGTTGGGATAACGAAGATAATTTTAGATCTTCTAGTGATATGAGGCGTAACCAATCAACAGGCGATGTTCGAGGTATGGGCGGCGGAGGGATGCCGTCTAGGTCTAGATCGACTGAGGATATTTATACACGGTCTCAATTGGAGGCTTCTGCTGCAAATAAAGATAATTTTTTTGCCCAGAAAATTGCTGAGAATGATTCAAGGCCTGAAGGAATTCCGCCATCACAAGGTGGAAAGTATGTTGGATTTGGGTCGAGTCCTGCACCTGCTCGAAGGAACGATCCACAAAATGATGTTTTCTCTGTTGTTTCTCAGGGGTTTGGTAAGTTATCTCTGGTTGCTGCTTCTGCTGCTCAATCGGCTGCAAATGCTGTTCAAGCTGGCACAAAAGAGCTGACTACCAAGGTGAAGGAGGGTGGCTATGATTACAAAGTAAATGAAACCGTCAATGTTGTCACAGCAAAAACAACAGAAATTGGGCAAAGGACATGGGGAATTATGAGAGGTGTAATGGCAATGGCTTCACAGAAGGTTGAAGAGTATGCCAAAGATGGCATGAACTGGAAGAATGATGGATGGCAACGGAACGAGAATGAGAAAAATGGATATTATCAAGAATTTGAGCATGATAACAAAGGATGGAATTCTTCTTCTGGAACAGGTCAGTCATCAGGAAGTGGACATCACAATAATTCTTACAACTCAAGTTCTTGGGATGATTGGGATACCAAAGACAACAGGAAAGAGGAAACAACAACCAAAGTGAGTGGAACCCataataataacaataacaacaacaacaataataatagcaatgatGGCTGGGCTGGCTGGGATGACCAAAAAGATGATGGATATGATCACTATTATCAAGCATCCGATCGGAAAACCGTTGGTCAAAATGGAAAAGCAGGTGGTGGTACATGGTCAGAGGGAGGCTTTCTCTGAGGTAGCATCTGAAATACACACCACAAGGTAGTTCCTGGTTTCCTCAAATGATGAAAATTTGAATTACTTGGAGTAGCTCATACAGATGGCTGGTCAACACATTGGTAAAGCTAAAGATGATGATTACAGGAACATATAGAATAGAGCTGATAAGATTCTTATGGAAGTGGATATCAAATCCCACTCATGTTTGTTTCAGGTCAGTACATCTTCAGttttcactttttcttttttctttttttcattttttttGGGAGGGGGGTGGGGGATCATGTTGTGTTTGCAAGTGCTGTATCATCAAAAAAACGTCGTCCATAGAGGTAAACCCGAATTATAGACACTGTTTATTATGTGGTTGGGAACA

Coding sequence (CDS)

ATGGCGGCGTCACGGCGGCTACGGGACCTTCAATCACGGCCGGGGAATAAGATCTGTGTTGATTGTTCCCAAAAGAATCCCCAATGGGCTTCTGTTTCTTATGGGGTTTTCATGTGTTTGGAATGTTCCGGGAAGCATCGTGGACTTGGTGTTCATATCTCTTTTGTTAGATCTGTTACTATGGATTCTTGGTCTGAGATCCAGATCAAGAAGATGGAGGCTGGAGGTAATGAGCAGCTTAATGCTTTCCTTTCCCAGTATGGAATACCTAAGGAGACTGATATTGTAACCAAATACAATACCAGTGCGGCTGGGGTTTATAGGGATCGGATTCAAGCTCTTGCTGAGGGTCGATCTTGGAAGGATCCACCTGCGGTGAAGGAGAATATTGGAGTAGGAAAATCCAGGCCTCCATTAGCGCAAAGTGCTGGTGGAGGAGGGAGTAAGGTGAATAATGGAGGTTGGGATAGTTGGGATAACGAAGATAATTTTAGATCTTCTAGTGATATGAGGCGTAACCAATCAACAGGCGATGTTCGAGGTATGGGCGGCGGAGGGATGCCGTCTAGGTCTAGATCGACTGAGGATATTTATACACGGTCTCAATTGGAGGCTTCTGCTGCAAATAAAGATAATTTTTTtGCCCAGAAAATTGCTGAGAATGATTCAAGGCCTGAAGGAATTCCGCCATCACAAGGTGGAAAGTATGTTGGATTTGGGTCGAGTCCTGCACCTGCTCGAAGGAACGATCCACAAAATGATGTTTTCTCTGTTGTTTCTCAGGGGTTTGGTAAGTTATCTCTGGTTGCTGCTTCTGCTGCTCAATCGGCTGCAAATGCTGTTCAAGCTGGCACAAAAGAGCTGACTACCAAGGTGAAGGAGGGTGGCTATGATTACAAAGTAAATGAAACCGTCAATGTTGTCACAGCAAAAACAACAGAAATTGGGCAAAGGACATGGGGAATTATGAGAGGTGTAATGGCAATGGCTTCACAGAAGGTTGAAGAGTATGCCAAAGATGGCATGAACTGGAAGAATGATGGATGGCAACGGAACGAGAATGAGAAAAATGGATATTATCAAGAATTTGAGCATGATAACAAAGGATGGAATTCTTCTTCTGGAACAGGTCAGTCATCAGGAAGTGGACATCACAATAATTCTTACAACTCAAGTTCTTGGGATGATTGGGATACCAAAGACAACAGGAAAGAGGAAACAACAACCAAAGTGAGTGGAACCCATAATAATAACAATAACAACAACAACAATAATAATAGCAATGATGGCTGGGCTGGCTGGGATGACCAAAAAGATGATGGATATGATCACTATTATCAAGCATCCGATCGGAAAACCGTTGGTCAAAATGGAAAAGCAGGTGGTGGTACATGGTCAGAGGGAGGCTTTCTCTGA

Protein sequence

MAASRRLRDLQSRPGNKICVDCSQKNPQWASVSYGVFMCLECSGKHRGLGVHISFVRSVTMDSWSEIQIKKMEAGGNEQLNAFLSQYGIPKETDIVTKYNTSAAGVYRDRIQALAEGRSWKDPPAVKENIGVGKSRPPLAQSAGGGGSKVNNGGWDSWDNEDNFRSSSDMRRNQSTGDVRGMGGGGMPSRSRSTEDIYTRSQLEASAANKDNFFAQKIAENDSRPEGIPPSQGGKYVGFGSSPAPARRNDPQNDVFSVVSQGFGKLSLVAASAAQSAANAVQAGTKELTTKVKEGGYDYKVNETVNVVTAKTTEIGQRTWGIMRGVMAMASQKVEEYAKDGMNWKNDGWQRNENEKNGYYQEFEHDNKGWNSSSGTGQSSGSGHHNNSYNSSSWDDWDTKDNRKEETTTKVSGTHNNNNNNNNNNNSNDGWAGWDDQKDDGYDHYYQASDRKTVGQNGKAGGGTWSEGGFL*
BLAST of Cucsa.175350 vs. Swiss-Prot
Match: AGD6_ARATH (Probable ADP-ribosylation factor GTPase-activating protein AGD6 OS=Arabidopsis thaliana GN=AGD6 PE=1 SV=1)

HSP 1 Score: 622.5 bits (1604), Expect = 3.9e-177
Identity = 315/478 (65.90%), Postives = 372/478 (77.82%), Query Frame = 1

Query: 1   MAASRRLRDLQSRPGNKICVDCSQKNPQWASVSYGVFMCLECSGKHRGLGVHISFVRSVT 60
           MAA+R+LR LQS+P NK+CVDC+QKNPQWASVSYG+FMCLECSGKHRGLGVHISFVRSVT
Sbjct: 1   MAATRQLRTLQSQPENKVCVDCAQKNPQWASVSYGIFMCLECSGKHRGLGVHISFVRSVT 60

Query: 61  MDSWSEIQIKKMEAGGNEQLNAFLSQYGIPKETDIVTKYNTSAAGVYRDRIQALAEGRSW 120
           MDSWS IQIKKMEAGGNE+LN F +QYGI KETDI++KYN++AA VYRDRIQALAEGR W
Sbjct: 61  MDSWSAIQIKKMEAGGNERLNKFFAQYGIAKETDIISKYNSNAASVYRDRIQALAEGRPW 120

Query: 121 KDPPAVKENIGVGKSRPPLAQSAGGGGSKVNNGGWDSWDNEDNFRSSSDMRRNQSTGDVR 180
            DPP VKE       +PPLAQ   G  +  NNGGWDSWDN+D+++S  DMRRNQS  D R
Sbjct: 121 NDPPVVKE----ANKKPPLAQGGYGNNNNNNNGGWDSWDNDDSYKS--DMRRNQSANDFR 180

Query: 181 GMGGG-GMPSRSRSTEDIYTRSQLEASAANKDNFFAQKIAENDSRPEGIPPSQGGKYVGF 240
             G   G   +S+S+EDIYTRSQLEASAA K++FFA+++AEN+S+PEG+PPSQGGKYVGF
Sbjct: 181 ASGNREGAHVKSKSSEDIYTRSQLEASAAGKESFFARRMAENESKPEGLPPSQGGKYVGF 240

Query: 241 GSSPAPARRNDPQNDVFSVVSQGFGKLSLVAASAAQSAANAVQAGTKELTTKVKEGGYDY 300
           GSS AP  RN+ Q+DVFSVVSQGFG+LSLVAASAAQSAA+ VQ GTKE T+KVKEGGYD+
Sbjct: 241 GSSSAPPPRNNQQDDVFSVVSQGFGRLSLVAASAAQSAASVVQTGTKEFTSKVKEGGYDH 300

Query: 301 KVNETVNVVTAKTTEIGQRTWGIMRGVMAMASQKVEEYAKDGMNWKNDGWQRNENEKNGY 360
           KV+ETVNVV  KTTEIG RTWGIM+GVMAMA+QKVEE+ K+G    N   Q++ENE NGY
Sbjct: 301 KVSETVNVVANKTTEIGHRTWGIMKGVMAMATQKVEEFTKEGSTSWN---QQSENEGNGY 360

Query: 361 YQEFEHDNKGWNSSSGTGQ---SSGSGHHNNSYNSSSWDDWDTKDNRKEETTTKVSGTHN 420
           YQ F + NK  NSS G G+   SS SGH+NNS NS+SWD W   +N+K E          
Sbjct: 361 YQNFGNGNKAANSSVGGGRPQSSSTSGHYNNSQNSNSWDSWGENENKKTEAVAP------ 420

Query: 421 NNNNNNNNNNSNDGWAGWDDQ--KDDGYD-HYYQASDRKTVGQNGKAGGGTWSEGGFL 472
                ++ +N +DGW GWDD   KDDG+D HY  A D+K+ G NGK+    W+ GGFL
Sbjct: 421 ---KGSSASNDDDGWTGWDDHDAKDDGFDGHYQSAGDKKSAGHNGKS-DTAWTGGGFL 459

BLAST of Cucsa.175350 vs. Swiss-Prot
Match: AGD7_ARATH (ADP-ribosylation factor GTPase-activating protein AGD7 OS=Arabidopsis thaliana GN=AGD7 PE=1 SV=1)

HSP 1 Score: 617.5 bits (1591), Expect = 1.2e-175
Identity = 314/477 (65.83%), Postives = 378/477 (79.25%), Query Frame = 1

Query: 1   MAASRRLRDLQSRPGNKICVDCSQKNPQWASVSYGVFMCLECSGKHRGLGVHISFVRSVT 60
           MAA+RRLR LQS+P NK+CVDCSQKNPQWAS+SYG+FMCLECSGKHRGLGVHISFVRSVT
Sbjct: 1   MAAARRLRTLQSQPENKVCVDCSQKNPQWASISYGIFMCLECSGKHRGLGVHISFVRSVT 60

Query: 61  MDSWSEIQIKKMEAGGNEQLNAFLSQYGIPKETDIVTKYNTSAAGVYRDRIQALAEGRSW 120
           MDSWSEIQIKKM+AGGNE+LN FL+QYGI KETDI++KYN++AA VYRDRIQALAEGR W
Sbjct: 61  MDSWSEIQIKKMDAGGNERLNNFLAQYGISKETDIISKYNSNAASVYRDRIQALAEGRQW 120

Query: 121 KDPPAVKENIGVG--KSRPPLAQSAGGGGSKVNNGGWDSWDNEDNFRSSSDMRRNQSTGD 180
           +DPP VKE++G G    +PPL+Q   GGG    NGGWD+WDN+D+FR S+DMRRNQS GD
Sbjct: 121 RDPPIVKESVGGGLMNKKPPLSQ---GGGRDSGNGGWDNWDNDDSFR-STDMRRNQSAGD 180

Query: 181 VRGMGGGGMPSRSRSTEDIYTRSQLEASAANKDNFFAQKIAENDSRPEGIPPSQGGKYVG 240
            R  GG G P++S+S+EDIY+RSQLEASAANK++FFA+++AEN+S+PEG+PPSQGGKYVG
Sbjct: 181 FRSSGGRGAPAKSKSSEDIYSRSQLEASAANKESFFAKRMAENESKPEGLPPSQGGKYVG 240

Query: 241 FGSSPAPARRNDPQN---DVFSVVSQGFGKLSLVAASAAQSAANAVQAGTKELTTKVKEG 300
           FGSSP PA R++ Q+   DVFSV+S+GFG+LSLVAA    SAAN VQ GT E T+KVKEG
Sbjct: 241 FGSSPGPAPRSNQQSGGGDVFSVMSEGFGRLSLVAA----SAANVVQTGTMEFTSKVKEG 300

Query: 301 GYDYKVNETVNVVTAKTTEIGQRTWGIMRGVMAMASQKVEEYAKDGMNWKNDGWQRNENE 360
           G D  V+ETVNVV +KTTEIGQRTWGIM+GVMA+ASQKVEE+ K+  +  N   Q+N+ E
Sbjct: 301 GLDQTVSETVNVVASKTTEIGQRTWGIMKGVMAIASQKVEEFTKEEASTWN---QQNKTE 360

Query: 361 KNGYYQEFEHDNKGWNSSSGTGQSSGSGHHNNSYNSSSWDDWDTKDNRKEETTTKVSGTH 420
            NGYYQ     NK  NSS G  QSS SGH+N+  NS+SWDDW  ++N K+E   KVS   
Sbjct: 361 GNGYYQNSGIGNKTANSSFGGSQSSSSGHNNSYRNSNSWDDWGEENNSKKEAAPKVS--- 420

Query: 421 NNNNNNNNNNNSNDGWAGWDDQKDDGYDHYYQ-ASDRKTVGQNGKAGGGTWSEGGFL 472
                  +N++ + GWAGWDD      D YYQ ASD+K+VG NGK+    W+ GGFL
Sbjct: 421 ------TSNDDDDGGWAGWDDNDAKDDDFYYQPASDKKSVGHNGKS-DTAWTGGGFL 456

BLAST of Cucsa.175350 vs. Swiss-Prot
Match: ARFG1_MOUSE (ADP-ribosylation factor GTPase-activating protein 1 OS=Mus musculus GN=Arfgap1 PE=1 SV=2)

HSP 1 Score: 144.1 bits (362), Expect = 4.0e-33
Identity = 113/419 (26.97%), Postives = 182/419 (43.44%), Query Frame = 1

Query: 7   LRDLQSRPGNKICVDCSQKNPQWASVSYGVFMCLECSGKHRGLGVHISFVRSVTMDSWSE 66
           L++++++  N +C +C   NPQW SV+YG+++CLECSG+HRGLGVH+SFVRSVTMD W +
Sbjct: 10  LKEVRAQDENNVCFECGAFNPQWVSVTYGIWICLECSGRHRGLGVHLSFVRSVTMDKWKD 69

Query: 67  IQIKKMEAGGNEQLNAFL-SQYGIPKETDIVTKYNTSAAGVYRDRIQALAEGRSWK---- 126
           I+++KM+AGGN +   FL +Q        +  KY++ AA ++RD++  LAEG+ W     
Sbjct: 70  IELEKMKAGGNAKFREFLETQDDYEPSWSLQDKYSSRAAALFRDKVATLAEGKEWSLESS 129

Query: 127 -----DPPAVKENIGVGKSRPPLAQSAGGGGSKVNNGGWDSWDNEDNFRSSSDMRRNQST 186
                 PP  K             QSA   G K     ++ W N+D   S    + N+  
Sbjct: 130 PAQNWTPPQPKTLQFTAHRASGQPQSAAASGDK----AFEDWLNDD-LGSYQGAQENRYV 189

Query: 187 GDVRGMGGGGMPSRSRSTEDIYTRSQLEASAANKDNF------FAQKIAENDSRPEGIPP 246
           G      G  +P + R  ED +  + + +  +   +F      FA    E  ++  G   
Sbjct: 190 G-----FGNTVPPQKR--EDDFLNNAMSSLYSGWSSFTTGASKFASAAKEGATK-FGSQA 249

Query: 247 SQGGKYVGFGSSP---APARRNDPQNDVFSVVSQGFGKLSLVAASAAQSAANAVQ---AG 306
           SQ    +G   +     PA+    +  +F  VS G  +L+             V    +G
Sbjct: 250 SQKASELGHSLNENVLKPAQEKVKEGRIFDDVSSGVSQLASKVQGVGSKGWRDVTTFFSG 309

Query: 307 TKELTTKVKEGGYDYKVNETVNVVTAKTTEIGQRTWGIMRGVMAMASQKVEEYAKDGMNW 366
             E ++     G+ Y+ +   N   +   +    T+G      A +         D    
Sbjct: 310 KAEDSSDRPLEGHSYQNSSGDNSQNSNIDQSFWETFGSAEPPKAKSPSSDSWTCADASTG 369

Query: 367 K--NDGWQ-----RNENEKNGYYQEFEHDNKGWNSSSGTGQSSGSGHHNNSYNSSSWDD 397
           +  +D W         N KN     +E     W  +SG G++  +     S     WD+
Sbjct: 370 RRSSDSWDVWGSGSASNNKNSNSDGWE----SWEGASGEGRAKATKKAAPSTADEGWDN 411


HSP 2 Score: 62.8 bits (151), Expect = 1.2e-08
Identity = 65/263 (24.71%), Postives = 110/263 (41.83%), Query Frame = 1

Query: 202 QLEASAANKDNFFAQKIAENDSRPEGIPPSQGGKYVGFGSSPAPARRNDP-QNDVFSVVS 261
           Q +++AA+ D  F   + ++    +G   +Q  +YVGFG++  P +R D   N+  S + 
Sbjct: 152 QPQSAAASGDKAFEDWLNDDLGSYQG---AQENRYVGFGNTVPPQKREDDFLNNAMSSLY 211

Query: 262 QGFGKLSLVA---ASAA---------QSAANAVQAG-------TKELTTKVKEGGYDYKV 321
            G+   +  A   ASAA         Q++  A + G        K    KVKEG     V
Sbjct: 212 SGWSSFTTGASKFASAAKEGATKFGSQASQKASELGHSLNENVLKPAQEKVKEGRIFDDV 271

Query: 322 NETVNVVTAKTTEIGQRTWGIMRGVMAMASQKVEEYAK---DGMNWKNDGWQRNENEKNG 381
           +  V+ + +K   +G + W   R V    S K E+ +    +G +++N     ++N    
Sbjct: 272 SSGVSQLASKVQGVGSKGW---RDVTTFFSGKAEDSSDRPLEGHSYQNSSGDNSQNS--- 331

Query: 382 YYQEFEHDNKGWNSSSGTGQSSGSGHHNNSYNSSSWDDWDTKDNRKEETTTKVSGTHNNN 441
                  D   W +       S       S +S SW   D    R+   +  V G+ + +
Sbjct: 332 -----NIDQSFWETF-----GSAEPPKAKSPSSDSWTCADASTGRRSSDSWDVWGSGSAS 391

BLAST of Cucsa.175350 vs. Swiss-Prot
Match: ARFG1_HUMAN (ADP-ribosylation factor GTPase-activating protein 1 OS=Homo sapiens GN=ARFGAP1 PE=1 SV=2)

HSP 1 Score: 143.3 bits (360), Expect = 6.9e-33
Identity = 95/298 (31.88%), Postives = 148/298 (49.66%), Query Frame = 1

Query: 7   LRDLQSRPGNKICVDCSQKNPQWASVSYGVFMCLECSGKHRGLGVHISFVRSVTMDSWSE 66
           L++++ +  N +C +C   NPQW SV+YG+++CLECSG+HRGLGVH+SFVRSVTMD W +
Sbjct: 10  LKEVRVQDENNVCFECGAFNPQWVSVTYGIWICLECSGRHRGLGVHLSFVRSVTMDKWKD 69

Query: 67  IQIKKMEAGGNEQLNAFL-SQYGIPKETDIVTKYNTSAAGVYRDRIQALAEGRSW--KDP 126
           I+++KM+AGGN +   FL SQ        +  KYN+ AA ++RD++ ALAEGR W  +  
Sbjct: 70  IELEKMKAGGNAKFREFLESQEDYDPCWSLQEKYNSRAAALFRDKVVALAEGREWSLESS 129

Query: 127 PAVKENIGVGKSRPPLAQSAGGGGSKV---NNGGWDSWDNEDNFRSSSDMRRNQSTGDVR 186
           PA        ++ P +     G    V   ++  ++ W N+       D+   Q     R
Sbjct: 130 PAQNWTPPQPRTLPSMVHRVSGQPQSVTASSDKAFEDWLND-------DLGSYQGAQGNR 189

Query: 187 GMGGGGMPSRSRSTEDIYTRSQLE-----ASAANKDNFFAQKIAENDSRPEGIPPSQGGK 246
            +G G  P   +  +D    +        +S     + FA    E  ++  G   SQ   
Sbjct: 190 YVGFGNTPPPQKKEDDFLNNAMSSLYSGWSSFTTGASRFASAAKEGATK-FGSQASQKAS 249

Query: 247 YVGFGSSP---APARRNDPQNDVFSVVSQGFGKLSLVAASAAQSAANAVQAGTKELTT 291
            +G   +     PA+    +  +F  VS G  +L    AS  Q   +    G +++TT
Sbjct: 250 ELGHSLNENVLKPAQEKVKEGKIFDDVSSGVSQL----ASKVQGVGS---KGWRDVTT 292


HSP 2 Score: 63.2 bits (152), Expect = 9.0e-09
Identity = 70/278 (25.18%), Postives = 116/278 (41.73%), Query Frame = 1

Query: 202 QLEASAANKDNFFAQKIAENDSRPEGIPPSQGGKYVGFGSSPAPARRNDP-QNDVFSVVS 261
           Q ++  A+ D  F   + ++    +G   +QG +YVGFG++P P ++ D   N+  S + 
Sbjct: 152 QPQSVTASSDKAFEDWLNDDLGSYQG---AQGNRYVGFGNTPPPQKKEDDFLNNAMSSLY 211

Query: 262 QGFGKLSLVA---ASAA---------QSAANAVQAG-------TKELTTKVKEGGYDYKV 321
            G+   +  A   ASAA         Q++  A + G        K    KVKEG     V
Sbjct: 212 SGWSSFTTGASRFASAAKEGATKFGSQASQKASELGHSLNENVLKPAQEKVKEGKIFDDV 271

Query: 322 NETVNVVTAKTTEIGQRTWGIMRGVMAMASQKVE---EYAKDGMNWKNDGWQRNENEKNG 381
           +  V+ + +K   +G + W   R V    S K E   +   +G +++N G          
Sbjct: 272 SSGVSQLASKVQGVGSKGW---RDVTTFFSGKAEGPLDSPSEGHSYQNSGLD-------- 331

Query: 382 YYQEFEHDNKGWNSSSGTGQSSGSGHHNNSYNSSSWDDW---DTKDNRKEETTTKVSGTH 441
           ++Q    D   W       ++ GS     +  S S D W   DT   R+   + +V G+ 
Sbjct: 332 HFQNSNIDQSFW-------ETFGSAEPTKTRKSPSSDSWTCADTSTERRSSDSWEVWGSA 391

Query: 442 NNNNNNNNNNNSNDGWAGWDDQK---------DDGYDH 445
           + N N+N     +DG  G +  K         DDG+D+
Sbjct: 392 STNRNSN-----SDGGEGGEGTKKAVPPAVPTDDGWDN 403

BLAST of Cucsa.175350 vs. Swiss-Prot
Match: ARFG1_RAT (ADP-ribosylation factor GTPase-activating protein 1 OS=Rattus norvegicus GN=Arfgap1 PE=1 SV=1)

HSP 1 Score: 142.5 bits (358), Expect = 1.2e-32
Identity = 113/420 (26.90%), Postives = 189/420 (45.00%), Query Frame = 1

Query: 7   LRDLQSRPGNKICVDCSQKNPQWASVSYGVFMCLECSGKHRGLGVHISFVRSVTMDSWSE 66
           L++++++  N +C +C   NPQW SV+YG+++CLECSG+HRGLGVH+SFVRSVTMD W +
Sbjct: 10  LKEVRAQDENNVCFECGAFNPQWVSVTYGIWICLECSGRHRGLGVHLSFVRSVTMDKWKD 69

Query: 67  IQIKKMEAGGNEQLNAFL-SQYGIPKETDIVTKYNTSAAGVYRDRIQALAEGRSW--KDP 126
           I+++KM+AGGN +   FL +Q        +  KY++ AA ++RD++  LAEG+ W  +  
Sbjct: 70  IELEKMKAGGNAKFREFLEAQDDYEPSWSLQDKYSSRAAALFRDKVATLAEGKEWSLESS 129

Query: 127 PAVKENIGVGKSRPPLAQSAGGGGSKVNNGG---WDSWDNEDNFRSSSDMRRNQSTGDVR 186
           PA        K+    A    G    V   G   ++ W N+D   S    + N+  G   
Sbjct: 130 PAQNWTPPQPKTLQFTAHRPAGQPQNVTTSGDKAFEDWLNDD-LGSYQGAQENRYVG--- 189

Query: 187 GMGGGGMPSRSRSTEDIYTRSQLEASAANKDNF------FAQKIAENDSRPEGIPPSQGG 246
              G  +P + R  ED +  S + +  +   +F      FA    E  ++  G   SQ  
Sbjct: 190 --FGNTVPPQKR--EDDFLNSAMSSLYSGWSSFTTGASKFASAAKEGATK-FGSQASQKA 249

Query: 247 KYVGFGSSP---APARRNDPQNDVFSVVSQGFGKLSLVAASAAQSAANAVQ---AGTKEL 306
             +G   +     PA+    +  +F  VS G  +L+             V    +G  E 
Sbjct: 250 SELGHSLNENVLKPAQEKVKEGRIFDDVSSGVSQLASKVQGVGSKGWRDVTTFFSGKAED 309

Query: 307 TTKVKEGGYDYKVNETVNVVTAKTTEIGQRTWGIMRGVMAMASQKVEEYAKDGMNWK--N 366
           T+     G+ Y+ +   N   +   +    T+G      A +         D    +  +
Sbjct: 310 TSDRPLEGHSYQNSSGDNSQNSTIDQSFWETFGSAEPPKAKSPSSDSWTCADASTGRRSS 369

Query: 367 DGWQ-----RNENEKNGYYQEFEHDNKGWNSSSGTGQSSGSGHHNNSYNSSSWDDWDTKD 402
           D W         N KN     +E     W  +SG G++  +     + ++++ + WD ++
Sbjct: 370 DSWDIWGSGSASNNKNSNSDGWE----SWEGASGEGRAKAT--KKAAPSTAADEGWDNQN 414

BLAST of Cucsa.175350 vs. TrEMBL
Match: A0A0A0LAL4_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_3G734360 PE=4 SV=1)

HSP 1 Score: 968.8 bits (2503), Expect = 2.4e-279
Identity = 468/471 (99.36%), Postives = 468/471 (99.36%), Query Frame = 1

Query: 1   MAASRRLRDLQSRPGNKICVDCSQKNPQWASVSYGVFMCLECSGKHRGLGVHISFVRSVT 60
           MAASRRLRDLQSRPGNKICVDCSQKNPQWASVSYGVFMCLECSGKHRGLGVHISFVRSVT
Sbjct: 1   MAASRRLRDLQSRPGNKICVDCSQKNPQWASVSYGVFMCLECSGKHRGLGVHISFVRSVT 60

Query: 61  MDSWSEIQIKKMEAGGNEQLNAFLSQYGIPKETDIVTKYNTSAAGVYRDRIQALAEGRSW 120
           MDSWSEIQIKKMEAGGNEQLNAFLSQYGIPKETDIVTKYNTSAAGVYRDRIQALAEGRSW
Sbjct: 61  MDSWSEIQIKKMEAGGNEQLNAFLSQYGIPKETDIVTKYNTSAAGVYRDRIQALAEGRSW 120

Query: 121 KDPPAVKENIGVGKSRPPLAQSAGGGGSKVNNGGWDSWDNEDNFRSSSDMRRNQSTGDVR 180
           KDPPAVKENIGVGKSRPPLAQSAGGGGSKVNNGGWD   NEDNFRSSSDMRRNQSTGDVR
Sbjct: 121 KDPPAVKENIGVGKSRPPLAQSAGGGGSKVNNGGWD---NEDNFRSSSDMRRNQSTGDVR 180

Query: 181 GMGGGGMPSRSRSTEDIYTRSQLEASAANKDNFFAQKIAENDSRPEGIPPSQGGKYVGFG 240
           GMGGGGMPSRSRSTEDIYTRSQLEASAANKDNFFAQKIAENDSRPEGIPPSQGGKYVGFG
Sbjct: 181 GMGGGGMPSRSRSTEDIYTRSQLEASAANKDNFFAQKIAENDSRPEGIPPSQGGKYVGFG 240

Query: 241 SSPAPARRNDPQNDVFSVVSQGFGKLSLVAASAAQSAANAVQAGTKELTTKVKEGGYDYK 300
           SSPAPARRNDPQNDVFSVVSQGFGKLSLVAASAAQSAANAVQAGTKELTTKVKEGGYDYK
Sbjct: 241 SSPAPARRNDPQNDVFSVVSQGFGKLSLVAASAAQSAANAVQAGTKELTTKVKEGGYDYK 300

Query: 301 VNETVNVVTAKTTEIGQRTWGIMRGVMAMASQKVEEYAKDGMNWKNDGWQRNENEKNGYY 360
           VNETVNVVTAKTTEIGQRTWGIMRGVMAMASQKVEEYAKDGMNWKNDGWQRNENEKNGYY
Sbjct: 301 VNETVNVVTAKTTEIGQRTWGIMRGVMAMASQKVEEYAKDGMNWKNDGWQRNENEKNGYY 360

Query: 361 QEFEHDNKGWNSSSGTGQSSGSGHHNNSYNSSSWDDWDTKDNRKEETTTKVSGTHNNNNN 420
           QEFEHDNKGWNSSSGTGQSSGSGHHNNSYNSSSWDDWDTKDNRKEETTTKVSGTHNNNNN
Sbjct: 361 QEFEHDNKGWNSSSGTGQSSGSGHHNNSYNSSSWDDWDTKDNRKEETTTKVSGTHNNNNN 420

Query: 421 NNNNNNSNDGWAGWDDQKDDGYDHYYQASDRKTVGQNGKAGGGTWSEGGFL 472
           NNNNNNSNDGWAGWDDQKDDGYDHYYQASDRKTVGQNGKAGGGTWSEGGFL
Sbjct: 421 NNNNNNSNDGWAGWDDQKDDGYDHYYQASDRKTVGQNGKAGGGTWSEGGFL 468

BLAST of Cucsa.175350 vs. TrEMBL
Match: A0A061ET55_THECC (ARF-GAP domain 7 isoform 1 OS=Theobroma cacao GN=TCM_021978 PE=4 SV=1)

HSP 1 Score: 726.9 bits (1875), Expect = 1.6e-206
Identity = 365/482 (75.73%), Postives = 414/482 (85.89%), Query Frame = 1

Query: 1   MAASRRLRDLQSRPGNKICVDCSQKNPQWASVSYGVFMCLECSGKHRGLGVHISFVRSVT 60
           MAA+RRLRDLQS+PGNK CVDC+QKNPQWASVSYG+FMCLECSGKHRGLGVHISFVRSVT
Sbjct: 1   MAATRRLRDLQSQPGNKTCVDCNQKNPQWASVSYGIFMCLECSGKHRGLGVHISFVRSVT 60

Query: 61  MDSWSEIQIKKMEAGGNEQLNAFLSQYGIPKETDIVTKYNTSAAGVYRDRIQALAEGRSW 120
           MDSWSEIQIKKME+GGNEQLN+FL+QYGIPKETDIVTKYNT+AA VYRDRIQALAEGR W
Sbjct: 61  MDSWSEIQIKKMESGGNEQLNSFLAQYGIPKETDIVTKYNTNAASVYRDRIQALAEGRPW 120

Query: 121 KDPPAVKE--NIGVGKSRPPLAQSAGGG-GSKV-NNGGWDSWDNEDNFRSSSDMRRNQST 180
           +DPP VKE  N G G  +PPL+   GGG GS   NNGGWDSWDN+D+FRSSSD+RRNQS 
Sbjct: 121 RDPPVVKETLNGGGGNRKPPLSGGGGGGRGSNYGNNGGWDSWDNDDSFRSSSDLRRNQSA 180

Query: 181 GDVRG---MGG--GGMPSRSRSTEDIYTRSQLEASAANKDNFFAQKIAENDSRPEGIPPS 240
            D RG    GG  GG P RSRSTED+YTRSQLEASAANK++FFA+K+AEN+SRP+G+PPS
Sbjct: 181 SDFRGGNNHGGQMGGAPVRSRSTEDMYTRSQLEASAANKESFFARKMAENESRPDGLPPS 240

Query: 241 QGGKYVGFGSSPAPARR-NDPQNDVFSVVSQGFGKLSLVAASAAQSAANAVQAGTKELTT 300
           QGGKYVGFGSSP P +R N+ Q DV SVVSQG GKLS+VAASAAQSAAN VQAGTKE T+
Sbjct: 241 QGGKYVGFGSSPMPTQRNNNSQGDVLSVVSQGLGKLSVVAASAAQSAANVVQAGTKEFTS 300

Query: 301 KVKEGGYDYKVNETVNVVTAKTTEIGQRTWGIMRGVMAMASQKVEEYAKDGMNWKNDGWQ 360
           KVKEGGYD +VNETVNVVTA+T+EIGQRTWGIM+GVMA+ASQKVEEY KDGMNWKN+ WQ
Sbjct: 301 KVKEGGYDTRVNETVNVVTARTSEIGQRTWGIMKGVMAIASQKVEEYTKDGMNWKNENWQ 360

Query: 361 RNENEKNGYYQEFEHDNKGWNSSSGTGQSSGSGHHNNSYNSSSWDDWDTKDNRKEETTTK 420
           RN++EKNGYYQEF+ +NKGWNS+SG GQSS  G + NSY+SSSWDDWD+KDNRK++ +TK
Sbjct: 361 RNDSEKNGYYQEFKQENKGWNSTSG-GQSSSGGSY-NSYSSSSWDDWDSKDNRKKD-STK 420

Query: 421 VSGTHNNNNNNNNNNNSNDGWAGWDDQKDDGYDHYYQ-ASDRKTVGQNGKAGGGTWSEGG 472
           V+            + SNDGWAGWDD KDDGYDH+Y  ASD+KTVG NGK+   TW+ GG
Sbjct: 421 VTA-----------SQSNDGWAGWDDAKDDGYDHFYSGASDKKTVGHNGKS-DATWTGGG 467

BLAST of Cucsa.175350 vs. TrEMBL
Match: A0A059A6A6_EUCGR (Uncharacterized protein OS=Eucalyptus grandis GN=EUGRSUZ_K03154 PE=4 SV=1)

HSP 1 Score: 719.2 bits (1855), Expect = 3.4e-204
Identity = 359/483 (74.33%), Postives = 403/483 (83.44%), Query Frame = 1

Query: 1   MAASRRLRDLQSRPGNKICVDCSQKNPQWASVSYGVFMCLECSGKHRGLGVHISFVRSVT 60
           MAASRRLRDLQS+P NK CVDC+QKNPQWASVSYGVFMCLECSGKHRGLGVHISFVRSVT
Sbjct: 1   MAASRRLRDLQSQPANKTCVDCAQKNPQWASVSYGVFMCLECSGKHRGLGVHISFVRSVT 60

Query: 61  MDSWSEIQIKKMEAGGNEQLNAFLSQYGIPKETDIVTKYNTSAAGVYRDRIQALAEGRSW 120
           MDSWSEIQIKKME GGNE+LNAFL+QYGIPKETDIV KYNT AAGVYRDRIQALAEGR W
Sbjct: 61  MDSWSEIQIKKMEVGGNERLNAFLAQYGIPKETDIVAKYNTKAAGVYRDRIQALAEGRPW 120

Query: 121 KDPPAVKENI----GVGKSRPPLAQSAGGGGSKVNNGGWDSWDNEDNFRSSSDMRRNQST 180
           +DPP VKE++    G G+SRPPLAQ  GGGG    +GGWDSWDN+D FRSSSD+RRNQS 
Sbjct: 121 RDPPVVKESVGGGGGGGRSRPPLAQKTGGGGGA--DGGWDSWDNDDGFRSSSDIRRNQSA 180

Query: 181 GDVRGMGGG----GMPSRSRSTEDIYTRSQLEASAANKDNFFAQKIAENDSRPEGIPPSQ 240
            D RG  GG    G P RS+ST+DIYTRSQLEASAANK+ FFAQKIAENDSRPEG+PPSQ
Sbjct: 181 SDFRGGAGGGGRSGAPVRSKSTQDIYTRSQLEASAANKETFFAQKIAENDSRPEGLPPSQ 240

Query: 241 GGKYVGFGSSPAPAR---RNDPQNDVFSVVSQGFGKLSLVAASAAQSAANAVQAGTKELT 300
           GGKYVGFGSSP P R    N+PQ DV S VSQG G+LS VAASAAQSAA+ VQAGTKE+T
Sbjct: 241 GGKYVGFGSSPVPPRMSNNNNPQGDVLSAVSQGLGRLSFVAASAAQSAASVVQAGTKEIT 300

Query: 301 TKVKEGGYDYKVNETVNVVTAKTTEIGQRTWGIMRGVMAMASQKVEEYAKDGMNWKNDGW 360
            KVKEGGYD+KVNETVNVVT KTTEIGQRTWGIM+GVMA+ASQKVEEY KDG++W N+ W
Sbjct: 301 AKVKEGGYDHKVNETVNVVTNKTTEIGQRTWGIMKGVMAIASQKVEEYTKDGVSWNNENW 360

Query: 361 QRNENEKNGYYQEFEHDNKGWNSSSGTGQSSGSGHHNNSYNSSSWDDWDTKDNRKEETTT 420
           QRN+NE+NGYYQEF+ +NKGWN+SSG GQSS SG +  +++S+SWDDWD KDN+KEETT 
Sbjct: 361 QRNDNERNGYYQEFKQENKGWNASSG-GQSSSSG-NVQTFSSTSWDDWDHKDNKKEETTK 420

Query: 421 KVSGTHNNNNNNNNNNNSNDGWAGWDDQKDDGYDHYYQASDRK-TVGQNGKAGGGTWSEG 472
              GT  N          NDGWAGWDD K+DG+D++YQ++  K T G NGK+   TW+EG
Sbjct: 421 --GGTSGN----------NDGWAGWDDAKEDGHDNWYQSAPNKGTAGHNGKS-DATWTEG 466

BLAST of Cucsa.175350 vs. TrEMBL
Match: F6HLG9_VITVI (Putative uncharacterized protein OS=Vitis vinifera GN=VIT_08s0007g00010 PE=4 SV=1)

HSP 1 Score: 716.8 bits (1849), Expect = 1.7e-203
Identity = 357/481 (74.22%), Postives = 404/481 (83.99%), Query Frame = 1

Query: 1   MAASRRLRDLQSRPGNKICVDCSQKNPQWASVSYGVFMCLECSGKHRGLGVHISFVRSVT 60
           MAASRRLRDLQS+P NKICVDCSQKNPQWASVSYG+FMCLECSGKHRGLGVHISFVRSVT
Sbjct: 1   MAASRRLRDLQSQPANKICVDCSQKNPQWASVSYGIFMCLECSGKHRGLGVHISFVRSVT 60

Query: 61  MDSWSEIQIKKMEAGGNEQLNAFLSQYGIPKETDIVTKYNTSAAGVYRDRIQALAEGRSW 120
           MDSWSEIQ+KKME+GGNE+LN+FL+QYGI KETDIVTKYNT AA +YRDRIQALAEGR W
Sbjct: 61  MDSWSEIQVKKMESGGNERLNSFLAQYGIKKETDIVTKYNTKAASIYRDRIQALAEGRPW 120

Query: 121 KDPPAVKENIGVGKSRPPLAQSAGGGGSK---VNNGGWDSWDNEDNFRSSSDMRRNQSTG 180
           +DPP VKE IG GKS+PPL QSAG GG      +NGGWDSWDN+D FR S+DMRRNQSTG
Sbjct: 121 RDPPVVKETIGAGKSKPPLGQSAGNGGRDGGFGDNGGWDSWDNDDGFR-STDMRRNQSTG 180

Query: 181 DVR---GMGGGGMPSRSRSTEDIYTRSQLEASAANKDNFFAQKIAENDSRPEGIPPSQGG 240
           DVR   G GG G P+RSRSTED+YTR++LEASAANK +FFA+K+AEN+SRPEGIPPSQGG
Sbjct: 181 DVRGGSGSGGRGFPARSRSTEDMYTRTELEASAANKGDFFARKLAENESRPEGIPPSQGG 240

Query: 241 KYVGFGSSPAPARRN---DPQNDVFSVVSQGFGKLSLVAASAAQSAANAVQAGTKELTTK 300
           KYVGFGS+P P + N   D   D  SVVSQG G+L+LVA SAAQSAANAVQAGTKEL++K
Sbjct: 241 KYVGFGSTPPPPQMNAQGDVLKDTVSVVSQGLGRLTLVATSAAQSAANAVQAGTKELSSK 300

Query: 301 VKEGGYDYKVNETVNVVTAKTTEIGQRTWGIMRGVMAMASQKVEEYAKDGMNWKNDGWQR 360
           V++GGYDYKVNETVNVVT KTTEIG +TWGIM+GVMAMASQKVEEYAKDGM W +DGWQR
Sbjct: 301 VRDGGYDYKVNETVNVVTTKTTEIGHKTWGIMKGVMAMASQKVEEYAKDGMKWNSDGWQR 360

Query: 361 NENEKNGYYQEFEHDNKGWNSSSGTGQSSGSGHHNNSYNSSSWDDWDTKDNRKEETTTKV 420
           N+NEKNG+YQEF   +KGWNSSS  GQ S SG H NS +SSSWDDWD KDN+KE++T   
Sbjct: 361 NDNEKNGFYQEFGQQSKGWNSSSEGGQPS-SGGHVNSVSSSSWDDWDQKDNKKEDSTKGA 420

Query: 421 SGTHNNNNNNNNNNNSNDGWAGWDDQKDDGYDHYYQ-ASDRKTVGQNGKAGGGTWSEGGF 472
           S  HN +N           WAGWDD KDDGY+++YQ ASD+K VG+NGK+    W+EGGF
Sbjct: 421 S--HNGDN-----------WAGWDDAKDDGYENFYQSASDKKAVGRNGKS-DTMWTEGGF 465

BLAST of Cucsa.175350 vs. TrEMBL
Match: M5WAQ9_PRUPE (Uncharacterized protein OS=Prunus persica GN=PRUPE_ppa005179mg PE=4 SV=1)

HSP 1 Score: 716.1 bits (1847), Expect = 2.9e-203
Identity = 364/490 (74.29%), Postives = 400/490 (81.63%), Query Frame = 1

Query: 1   MAASRRLRDLQSRPGNKICVDCSQKNPQWASVSYGVFMCLECSGKHRGLGVHISFVRSVT 60
           MAASRRLRDLQS+PGNKICVDCSQKNPQWASVSYGVFMCLECSGKHRGLGVHISFVRSVT
Sbjct: 1   MAASRRLRDLQSQPGNKICVDCSQKNPQWASVSYGVFMCLECSGKHRGLGVHISFVRSVT 60

Query: 61  MDSWSEIQIKKMEAGGNEQLNAFLSQYGIPKETDIVTKYNTSAAGVYRDRIQALAEGRSW 120
           MDSWSEIQIKKMEAGGNEQLNAFL+ YG+ KETDIVTKYNT+AA VYRDRIQALAEGR W
Sbjct: 61  MDSWSEIQIKKMEAGGNEQLNAFLASYGVSKETDIVTKYNTNAASVYRDRIQALAEGRPW 120

Query: 121 KDPPAVKENIGVGKSRPPLAQSAGGGGSK----VNNGGWDSWDNEDNFRSSSDMRRNQST 180
           +DPP VKE +G GKS+PPLA   GGGGS+     NNGGWDSWD +D FRSS+D+RRNQST
Sbjct: 121 RDPPVVKETLGFGKSKPPLAHGGGGGGSRGGNSGNNGGWDSWD-DDGFRSSNDIRRNQST 180

Query: 181 GDVRGMGGGG------------MPSRSRSTEDIYTRSQLEASAANKDNFFAQKIAENDSR 240
           GD R  GGGG            MP+RSRSTEDI TRSQ EASAANK++FFA+KIAEN+SR
Sbjct: 181 GDFRAGGGGGHGGRGDGGYGGSMPARSRSTEDI-TRSQFEASAANKESFFARKIAENESR 240

Query: 241 PEGIPPSQGGKYVGFGSSPAPARRN---DPQNDVFSVVSQGFGKLSLVAASAAQSAANAV 300
           P+G+PPSQGGKYVGFGSSPAP++ +   + Q DV SVVSQG GKLSLVAASAAQSAA+ V
Sbjct: 241 PDGLPPSQGGKYVGFGSSPAPSQNHQNVNAQGDVLSVVSQGIGKLSLVAASAAQSAASVV 300

Query: 301 QAGTKELTTKVKEGGYDYKVNETVNVVTAKTTEIGQRTWGIMRGVMAMASQKVEEYAKDG 360
           QAGTKELTTKVKEGGYDYKVNETVNVVTAKTTEIG RTWGIM+GVMAMASQKVEEY KDG
Sbjct: 301 QAGTKELTTKVKEGGYDYKVNETVNVVTAKTTEIGHRTWGIMKGVMAMASQKVEEYTKDG 360

Query: 361 MNWKND-GWQRNENEKNGYYQEFEHDNKGWNSSSGTGQSSGSGHHNNSYNSSSWDDWDTK 420
             WK D  WQRN++E+NGYYQEF+ +NK WNSS G   SSG   + NSY SSSWDDWD K
Sbjct: 361 TAWKADNNWQRNDSEQNGYYQEFKQENKQWNSSGGGQSSSG---NFNSYGSSSWDDWDQK 420

Query: 421 DNRKEETTTKVSGTHNNNNNNNNNNNSNDGWAGWDDQKDDGYDHYYQASDRKTVGQNGKA 471
           DNRK+ETT                +NS+DGWAGWDD KDDG+DHY  AS  K  G NGK+
Sbjct: 421 DNRKQETT------------KGTGSNSSDGWAGWDDGKDDGFDHYQSASVSKAAGHNGKS 472

BLAST of Cucsa.175350 vs. TAIR10
Match: AT3G53710.1 (AT3G53710.1 ARF-GAP domain 6)

HSP 1 Score: 622.5 bits (1604), Expect = 2.2e-178
Identity = 315/478 (65.90%), Postives = 372/478 (77.82%), Query Frame = 1

Query: 1   MAASRRLRDLQSRPGNKICVDCSQKNPQWASVSYGVFMCLECSGKHRGLGVHISFVRSVT 60
           MAA+R+LR LQS+P NK+CVDC+QKNPQWASVSYG+FMCLECSGKHRGLGVHISFVRSVT
Sbjct: 1   MAATRQLRTLQSQPENKVCVDCAQKNPQWASVSYGIFMCLECSGKHRGLGVHISFVRSVT 60

Query: 61  MDSWSEIQIKKMEAGGNEQLNAFLSQYGIPKETDIVTKYNTSAAGVYRDRIQALAEGRSW 120
           MDSWS IQIKKMEAGGNE+LN F +QYGI KETDI++KYN++AA VYRDRIQALAEGR W
Sbjct: 61  MDSWSAIQIKKMEAGGNERLNKFFAQYGIAKETDIISKYNSNAASVYRDRIQALAEGRPW 120

Query: 121 KDPPAVKENIGVGKSRPPLAQSAGGGGSKVNNGGWDSWDNEDNFRSSSDMRRNQSTGDVR 180
            DPP VKE       +PPLAQ   G  +  NNGGWDSWDN+D+++S  DMRRNQS  D R
Sbjct: 121 NDPPVVKE----ANKKPPLAQGGYGNNNNNNNGGWDSWDNDDSYKS--DMRRNQSANDFR 180

Query: 181 GMGGG-GMPSRSRSTEDIYTRSQLEASAANKDNFFAQKIAENDSRPEGIPPSQGGKYVGF 240
             G   G   +S+S+EDIYTRSQLEASAA K++FFA+++AEN+S+PEG+PPSQGGKYVGF
Sbjct: 181 ASGNREGAHVKSKSSEDIYTRSQLEASAAGKESFFARRMAENESKPEGLPPSQGGKYVGF 240

Query: 241 GSSPAPARRNDPQNDVFSVVSQGFGKLSLVAASAAQSAANAVQAGTKELTTKVKEGGYDY 300
           GSS AP  RN+ Q+DVFSVVSQGFG+LSLVAASAAQSAA+ VQ GTKE T+KVKEGGYD+
Sbjct: 241 GSSSAPPPRNNQQDDVFSVVSQGFGRLSLVAASAAQSAASVVQTGTKEFTSKVKEGGYDH 300

Query: 301 KVNETVNVVTAKTTEIGQRTWGIMRGVMAMASQKVEEYAKDGMNWKNDGWQRNENEKNGY 360
           KV+ETVNVV  KTTEIG RTWGIM+GVMAMA+QKVEE+ K+G    N   Q++ENE NGY
Sbjct: 301 KVSETVNVVANKTTEIGHRTWGIMKGVMAMATQKVEEFTKEGSTSWN---QQSENEGNGY 360

Query: 361 YQEFEHDNKGWNSSSGTGQ---SSGSGHHNNSYNSSSWDDWDTKDNRKEETTTKVSGTHN 420
           YQ F + NK  NSS G G+   SS SGH+NNS NS+SWD W   +N+K E          
Sbjct: 361 YQNFGNGNKAANSSVGGGRPQSSSTSGHYNNSQNSNSWDSWGENENKKTEAVAP------ 420

Query: 421 NNNNNNNNNNSNDGWAGWDDQ--KDDGYD-HYYQASDRKTVGQNGKAGGGTWSEGGFL 472
                ++ +N +DGW GWDD   KDDG+D HY  A D+K+ G NGK+    W+ GGFL
Sbjct: 421 ---KGSSASNDDDGWTGWDDHDAKDDGFDGHYQSAGDKKSAGHNGKS-DTAWTGGGFL 459

BLAST of Cucsa.175350 vs. TAIR10
Match: AT2G37550.1 (AT2G37550.1 ARF-GAP domain 7)

HSP 1 Score: 617.5 bits (1591), Expect = 7.0e-177
Identity = 314/477 (65.83%), Postives = 378/477 (79.25%), Query Frame = 1

Query: 1   MAASRRLRDLQSRPGNKICVDCSQKNPQWASVSYGVFMCLECSGKHRGLGVHISFVRSVT 60
           MAA+RRLR LQS+P NK+CVDCSQKNPQWAS+SYG+FMCLECSGKHRGLGVHISFVRSVT
Sbjct: 1   MAAARRLRTLQSQPENKVCVDCSQKNPQWASISYGIFMCLECSGKHRGLGVHISFVRSVT 60

Query: 61  MDSWSEIQIKKMEAGGNEQLNAFLSQYGIPKETDIVTKYNTSAAGVYRDRIQALAEGRSW 120
           MDSWSEIQIKKM+AGGNE+LN FL+QYGI KETDI++KYN++AA VYRDRIQALAEGR W
Sbjct: 61  MDSWSEIQIKKMDAGGNERLNNFLAQYGISKETDIISKYNSNAASVYRDRIQALAEGRQW 120

Query: 121 KDPPAVKENIGVG--KSRPPLAQSAGGGGSKVNNGGWDSWDNEDNFRSSSDMRRNQSTGD 180
           +DPP VKE++G G    +PPL+Q   GGG    NGGWD+WDN+D+FR S+DMRRNQS GD
Sbjct: 121 RDPPIVKESVGGGLMNKKPPLSQ---GGGRDSGNGGWDNWDNDDSFR-STDMRRNQSAGD 180

Query: 181 VRGMGGGGMPSRSRSTEDIYTRSQLEASAANKDNFFAQKIAENDSRPEGIPPSQGGKYVG 240
            R  GG G P++S+S+EDIY+RSQLEASAANK++FFA+++AEN+S+PEG+PPSQGGKYVG
Sbjct: 181 FRSSGGRGAPAKSKSSEDIYSRSQLEASAANKESFFAKRMAENESKPEGLPPSQGGKYVG 240

Query: 241 FGSSPAPARRNDPQN---DVFSVVSQGFGKLSLVAASAAQSAANAVQAGTKELTTKVKEG 300
           FGSSP PA R++ Q+   DVFSV+S+GFG+LSLVAA    SAAN VQ GT E T+KVKEG
Sbjct: 241 FGSSPGPAPRSNQQSGGGDVFSVMSEGFGRLSLVAA----SAANVVQTGTMEFTSKVKEG 300

Query: 301 GYDYKVNETVNVVTAKTTEIGQRTWGIMRGVMAMASQKVEEYAKDGMNWKNDGWQRNENE 360
           G D  V+ETVNVV +KTTEIGQRTWGIM+GVMA+ASQKVEE+ K+  +  N   Q+N+ E
Sbjct: 301 GLDQTVSETVNVVASKTTEIGQRTWGIMKGVMAIASQKVEEFTKEEASTWN---QQNKTE 360

Query: 361 KNGYYQEFEHDNKGWNSSSGTGQSSGSGHHNNSYNSSSWDDWDTKDNRKEETTTKVSGTH 420
            NGYYQ     NK  NSS G  QSS SGH+N+  NS+SWDDW  ++N K+E   KVS   
Sbjct: 361 GNGYYQNSGIGNKTANSSFGGSQSSSSGHNNSYRNSNSWDDWGEENNSKKEAAPKVS--- 420

Query: 421 NNNNNNNNNNNSNDGWAGWDDQKDDGYDHYYQ-ASDRKTVGQNGKAGGGTWSEGGFL 472
                  +N++ + GWAGWDD      D YYQ ASD+K+VG NGK+    W+ GGFL
Sbjct: 421 ------TSNDDDDGGWAGWDDNDAKDDDFYYQPASDKKSVGHNGKS-DTAWTGGGFL 456

BLAST of Cucsa.175350 vs. TAIR10
Match: AT4G17890.1 (AT4G17890.1 ARF-GAP domain 8)

HSP 1 Score: 120.2 bits (300), Expect = 3.5e-27
Identity = 62/135 (45.93%), Postives = 81/135 (60.00%), Query Frame = 1

Query: 8   RDLQSRPGNKICVDCSQKNPQWASVSYGVFMCLECSGKHRGLGVHISFVRSVTMDSWSEI 67
           R L+S+  NK+C DCS KNP WASV+YG+F+C++CS  HR LGVHISFVRS  +DSWS  
Sbjct: 17  RKLKSKSENKVCFDCSAKNPTWASVTYGIFLCIDCSATHRNLGVHISFVRSTNLDSWSPE 76

Query: 68  QIKKMEAGGNEQLNAFLSQYGIPKETDIVTKYNTSAAGVYRDRIQALAEGRSWKDPPAVK 127
           Q++ M  GGN +   F  Q+G      I  KY + AA +YR   Q LA+    +   A+ 
Sbjct: 77  QLRTMMFGGNNRAQVFFKQHGWTDGGKIEAKYTSRAADLYR---QILAK----EVAKAIA 136

Query: 128 ENIGVGKSRPPLAQS 143
           E    G    P+A S
Sbjct: 137 EETNSGLLSSPVATS 144

BLAST of Cucsa.175350 vs. TAIR10
Match: AT5G46750.1 (AT5G46750.1 ARF-GAP domain 9)

HSP 1 Score: 117.9 bits (294), Expect = 1.7e-26
Identity = 53/104 (50.96%), Postives = 68/104 (65.38%), Query Frame = 1

Query: 8   RDLQSRPGNKICVDCSQKNPQWASVSYGVFMCLECSGKHRGLGVHISFVRSVTMDSWSEI 67
           R L+S+  NK+C DCS KNP WASV YG+F+C++CS  HR LGVHISFVRS  +DSWS  
Sbjct: 14  RKLKSKSENKVCFDCSAKNPTWASVPYGIFLCIDCSAVHRSLGVHISFVRSTNLDSWSPE 73

Query: 68  QIKKMEAGGNEQLNAFLSQYGIPKETDIVTKYNTSAAGVYRDRI 112
           Q++ M  GGN +   F  Q+G      I  KY + AA +YR  +
Sbjct: 74  QLRTMMFGGNNRAQVFFKQHGWNDGGKIEAKYTSRAADMYRQTL 117

BLAST of Cucsa.175350 vs. TAIR10
Match: AT2G35210.1 (AT2G35210.1 root and pollen arfgap)

HSP 1 Score: 115.9 bits (289), Expect = 6.6e-26
Identity = 51/101 (50.50%), Postives = 67/101 (66.34%), Query Frame = 1

Query: 8   RDLQSRPGNKICVDCSQKNPQWASVSYGVFMCLECSGKHRGLGVHISFVRSVTMDSWSEI 67
           + L+++  NKIC DC+ KNP WASV+YG+F+C++CS  HR LGVHISFVRS  +DSWS  
Sbjct: 14  KKLKAKSDNKICFDCNAKNPTWASVTYGIFLCIDCSAVHRSLGVHISFVRSTNLDSWSSE 73

Query: 68  QIKKMEAGGNEQLNAFLSQYGIPKETDIVTKYNTSAAGVYR 109
           Q+K M  GGN +   F  QYG         KY + AA +Y+
Sbjct: 74  QLKMMIYGGNNRAQVFFKQYGWSDGGKTEAKYTSRAADLYK 114

BLAST of Cucsa.175350 vs. NCBI nr
Match: gi|778683566|ref|XP_011651902.1| (PREDICTED: ADP-ribosylation factor GTPase-activating protein AGD7 [Cucumis sativus])

HSP 1 Score: 968.8 bits (2503), Expect = 3.5e-279
Identity = 468/471 (99.36%), Postives = 468/471 (99.36%), Query Frame = 1

Query: 1   MAASRRLRDLQSRPGNKICVDCSQKNPQWASVSYGVFMCLECSGKHRGLGVHISFVRSVT 60
           MAASRRLRDLQSRPGNKICVDCSQKNPQWASVSYGVFMCLECSGKHRGLGVHISFVRSVT
Sbjct: 1   MAASRRLRDLQSRPGNKICVDCSQKNPQWASVSYGVFMCLECSGKHRGLGVHISFVRSVT 60

Query: 61  MDSWSEIQIKKMEAGGNEQLNAFLSQYGIPKETDIVTKYNTSAAGVYRDRIQALAEGRSW 120
           MDSWSEIQIKKMEAGGNEQLNAFLSQYGIPKETDIVTKYNTSAAGVYRDRIQALAEGRSW
Sbjct: 61  MDSWSEIQIKKMEAGGNEQLNAFLSQYGIPKETDIVTKYNTSAAGVYRDRIQALAEGRSW 120

Query: 121 KDPPAVKENIGVGKSRPPLAQSAGGGGSKVNNGGWDSWDNEDNFRSSSDMRRNQSTGDVR 180
           KDPPAVKENIGVGKSRPPLAQSAGGGGSKVNNGGWD   NEDNFRSSSDMRRNQSTGDVR
Sbjct: 121 KDPPAVKENIGVGKSRPPLAQSAGGGGSKVNNGGWD---NEDNFRSSSDMRRNQSTGDVR 180

Query: 181 GMGGGGMPSRSRSTEDIYTRSQLEASAANKDNFFAQKIAENDSRPEGIPPSQGGKYVGFG 240
           GMGGGGMPSRSRSTEDIYTRSQLEASAANKDNFFAQKIAENDSRPEGIPPSQGGKYVGFG
Sbjct: 181 GMGGGGMPSRSRSTEDIYTRSQLEASAANKDNFFAQKIAENDSRPEGIPPSQGGKYVGFG 240

Query: 241 SSPAPARRNDPQNDVFSVVSQGFGKLSLVAASAAQSAANAVQAGTKELTTKVKEGGYDYK 300
           SSPAPARRNDPQNDVFSVVSQGFGKLSLVAASAAQSAANAVQAGTKELTTKVKEGGYDYK
Sbjct: 241 SSPAPARRNDPQNDVFSVVSQGFGKLSLVAASAAQSAANAVQAGTKELTTKVKEGGYDYK 300

Query: 301 VNETVNVVTAKTTEIGQRTWGIMRGVMAMASQKVEEYAKDGMNWKNDGWQRNENEKNGYY 360
           VNETVNVVTAKTTEIGQRTWGIMRGVMAMASQKVEEYAKDGMNWKNDGWQRNENEKNGYY
Sbjct: 301 VNETVNVVTAKTTEIGQRTWGIMRGVMAMASQKVEEYAKDGMNWKNDGWQRNENEKNGYY 360

Query: 361 QEFEHDNKGWNSSSGTGQSSGSGHHNNSYNSSSWDDWDTKDNRKEETTTKVSGTHNNNNN 420
           QEFEHDNKGWNSSSGTGQSSGSGHHNNSYNSSSWDDWDTKDNRKEETTTKVSGTHNNNNN
Sbjct: 361 QEFEHDNKGWNSSSGTGQSSGSGHHNNSYNSSSWDDWDTKDNRKEETTTKVSGTHNNNNN 420

Query: 421 NNNNNNSNDGWAGWDDQKDDGYDHYYQASDRKTVGQNGKAGGGTWSEGGFL 472
           NNNNNNSNDGWAGWDDQKDDGYDHYYQASDRKTVGQNGKAGGGTWSEGGFL
Sbjct: 421 NNNNNNSNDGWAGWDDQKDDGYDHYYQASDRKTVGQNGKAGGGTWSEGGFL 468

BLAST of Cucsa.175350 vs. NCBI nr
Match: gi|659083832|ref|XP_008442564.1| (PREDICTED: ADP-ribosylation factor GTPase-activating protein AGD7 [Cucumis melo])

HSP 1 Score: 955.7 bits (2469), Expect = 3.1e-275
Identity = 460/471 (97.66%), Postives = 464/471 (98.51%), Query Frame = 1

Query: 1   MAASRRLRDLQSRPGNKICVDCSQKNPQWASVSYGVFMCLECSGKHRGLGVHISFVRSVT 60
           MAASRRLRDLQSRPGNKICVDCSQKNPQWASVSYGVFMCLECSGKHRGLGVHISFVRSVT
Sbjct: 1   MAASRRLRDLQSRPGNKICVDCSQKNPQWASVSYGVFMCLECSGKHRGLGVHISFVRSVT 60

Query: 61  MDSWSEIQIKKMEAGGNEQLNAFLSQYGIPKETDIVTKYNTSAAGVYRDRIQALAEGRSW 120
           MDSWSEIQIKKMEAGGNEQLNAFL+QYGIPKETDIVTKYNTSAAGVYRDRIQALAEGRSW
Sbjct: 61  MDSWSEIQIKKMEAGGNEQLNAFLAQYGIPKETDIVTKYNTSAAGVYRDRIQALAEGRSW 120

Query: 121 KDPPAVKENIGVGKSRPPLAQSAGGGGSKVNNGGWDSWDNEDNFRSSSDMRRNQSTGDVR 180
           KDPPAVKENIGVGKSRPPLAQSAGGGGSKVNNGGWDSWDN+DNFRSSSDMRRNQSTGDVR
Sbjct: 121 KDPPAVKENIGVGKSRPPLAQSAGGGGSKVNNGGWDSWDNDDNFRSSSDMRRNQSTGDVR 180

Query: 181 GMGGGGMPSRSRSTEDIYTRSQLEASAANKDNFFAQKIAENDSRPEGIPPSQGGKYVGFG 240
           G GGGGMPSRSRSTEDIYTR+QLEASAANKDNFFA+KIAENDSRPEGIPPSQGGKYVGFG
Sbjct: 181 GTGGGGMPSRSRSTEDIYTRAQLEASAANKDNFFARKIAENDSRPEGIPPSQGGKYVGFG 240

Query: 241 SSPAPARRNDPQNDVFSVVSQGFGKLSLVAASAAQSAANAVQAGTKELTTKVKEGGYDYK 300
           SSP PARRNDPQNDVFSVVSQGFGKLSLVAASAAQSAANAVQAGTKELTTKVKEGGYDYK
Sbjct: 241 SSPTPARRNDPQNDVFSVVSQGFGKLSLVAASAAQSAANAVQAGTKELTTKVKEGGYDYK 300

Query: 301 VNETVNVVTAKTTEIGQRTWGIMRGVMAMASQKVEEYAKDGMNWKNDGWQRNENEKNGYY 360
           VNETVNVVTAKTTEIGQRTWGIMRGVMAMASQKVEEYAKDGMNWKNDGWQRNENEKNGYY
Sbjct: 301 VNETVNVVTAKTTEIGQRTWGIMRGVMAMASQKVEEYAKDGMNWKNDGWQRNENEKNGYY 360

Query: 361 QEFEHDNKGWNSSSGTGQSSGSGHHNNSYNSSSWDDWDTKDNRKEETTTKVSGTHNNNNN 420
           QEFEHDNKGWNSSSGTGQSSGSGHHNNSYNSSSWDDWDTKDNRKEETTTKVSGTH     
Sbjct: 361 QEFEHDNKGWNSSSGTGQSSGSGHHNNSYNSSSWDDWDTKDNRKEETTTKVSGTH----- 420

Query: 421 NNNNNNSNDGWAGWDDQKDDGYDHYYQASDRKTVGQNGKAGGGTWSEGGFL 472
           NNNNNNSNDGWAGWDDQKDDGYDHYYQASDRKTVGQNGKAGGGTWSEGGFL
Sbjct: 421 NNNNNNSNDGWAGWDDQKDDGYDHYYQASDRKTVGQNGKAGGGTWSEGGFL 466

BLAST of Cucsa.175350 vs. NCBI nr
Match: gi|590629759|ref|XP_007027080.1| (ARF-GAP domain 7 isoform 1 [Theobroma cacao])

HSP 1 Score: 726.9 bits (1875), Expect = 2.3e-206
Identity = 365/482 (75.73%), Postives = 414/482 (85.89%), Query Frame = 1

Query: 1   MAASRRLRDLQSRPGNKICVDCSQKNPQWASVSYGVFMCLECSGKHRGLGVHISFVRSVT 60
           MAA+RRLRDLQS+PGNK CVDC+QKNPQWASVSYG+FMCLECSGKHRGLGVHISFVRSVT
Sbjct: 1   MAATRRLRDLQSQPGNKTCVDCNQKNPQWASVSYGIFMCLECSGKHRGLGVHISFVRSVT 60

Query: 61  MDSWSEIQIKKMEAGGNEQLNAFLSQYGIPKETDIVTKYNTSAAGVYRDRIQALAEGRSW 120
           MDSWSEIQIKKME+GGNEQLN+FL+QYGIPKETDIVTKYNT+AA VYRDRIQALAEGR W
Sbjct: 61  MDSWSEIQIKKMESGGNEQLNSFLAQYGIPKETDIVTKYNTNAASVYRDRIQALAEGRPW 120

Query: 121 KDPPAVKE--NIGVGKSRPPLAQSAGGG-GSKV-NNGGWDSWDNEDNFRSSSDMRRNQST 180
           +DPP VKE  N G G  +PPL+   GGG GS   NNGGWDSWDN+D+FRSSSD+RRNQS 
Sbjct: 121 RDPPVVKETLNGGGGNRKPPLSGGGGGGRGSNYGNNGGWDSWDNDDSFRSSSDLRRNQSA 180

Query: 181 GDVRG---MGG--GGMPSRSRSTEDIYTRSQLEASAANKDNFFAQKIAENDSRPEGIPPS 240
            D RG    GG  GG P RSRSTED+YTRSQLEASAANK++FFA+K+AEN+SRP+G+PPS
Sbjct: 181 SDFRGGNNHGGQMGGAPVRSRSTEDMYTRSQLEASAANKESFFARKMAENESRPDGLPPS 240

Query: 241 QGGKYVGFGSSPAPARR-NDPQNDVFSVVSQGFGKLSLVAASAAQSAANAVQAGTKELTT 300
           QGGKYVGFGSSP P +R N+ Q DV SVVSQG GKLS+VAASAAQSAAN VQAGTKE T+
Sbjct: 241 QGGKYVGFGSSPMPTQRNNNSQGDVLSVVSQGLGKLSVVAASAAQSAANVVQAGTKEFTS 300

Query: 301 KVKEGGYDYKVNETVNVVTAKTTEIGQRTWGIMRGVMAMASQKVEEYAKDGMNWKNDGWQ 360
           KVKEGGYD +VNETVNVVTA+T+EIGQRTWGIM+GVMA+ASQKVEEY KDGMNWKN+ WQ
Sbjct: 301 KVKEGGYDTRVNETVNVVTARTSEIGQRTWGIMKGVMAIASQKVEEYTKDGMNWKNENWQ 360

Query: 361 RNENEKNGYYQEFEHDNKGWNSSSGTGQSSGSGHHNNSYNSSSWDDWDTKDNRKEETTTK 420
           RN++EKNGYYQEF+ +NKGWNS+SG GQSS  G + NSY+SSSWDDWD+KDNRK++ +TK
Sbjct: 361 RNDSEKNGYYQEFKQENKGWNSTSG-GQSSSGGSY-NSYSSSSWDDWDSKDNRKKD-STK 420

Query: 421 VSGTHNNNNNNNNNNNSNDGWAGWDDQKDDGYDHYYQ-ASDRKTVGQNGKAGGGTWSEGG 472
           V+            + SNDGWAGWDD KDDGYDH+Y  ASD+KTVG NGK+   TW+ GG
Sbjct: 421 VTA-----------SQSNDGWAGWDDAKDDGYDHFYSGASDKKTVGHNGKS-DATWTGGG 467

BLAST of Cucsa.175350 vs. NCBI nr
Match: gi|702499008|ref|XP_010037851.1| (PREDICTED: probable ADP-ribosylation factor GTPase-activating protein AGD6 [Eucalyptus grandis])

HSP 1 Score: 719.2 bits (1855), Expect = 4.8e-204
Identity = 359/483 (74.33%), Postives = 403/483 (83.44%), Query Frame = 1

Query: 1   MAASRRLRDLQSRPGNKICVDCSQKNPQWASVSYGVFMCLECSGKHRGLGVHISFVRSVT 60
           MAASRRLRDLQS+P NK CVDC+QKNPQWASVSYGVFMCLECSGKHRGLGVHISFVRSVT
Sbjct: 1   MAASRRLRDLQSQPANKTCVDCAQKNPQWASVSYGVFMCLECSGKHRGLGVHISFVRSVT 60

Query: 61  MDSWSEIQIKKMEAGGNEQLNAFLSQYGIPKETDIVTKYNTSAAGVYRDRIQALAEGRSW 120
           MDSWSEIQIKKME GGNE+LNAFL+QYGIPKETDIV KYNT AAGVYRDRIQALAEGR W
Sbjct: 61  MDSWSEIQIKKMEVGGNERLNAFLAQYGIPKETDIVAKYNTKAAGVYRDRIQALAEGRPW 120

Query: 121 KDPPAVKENI----GVGKSRPPLAQSAGGGGSKVNNGGWDSWDNEDNFRSSSDMRRNQST 180
           +DPP VKE++    G G+SRPPLAQ  GGGG    +GGWDSWDN+D FRSSSD+RRNQS 
Sbjct: 121 RDPPVVKESVGGGGGGGRSRPPLAQKTGGGGGA--DGGWDSWDNDDGFRSSSDIRRNQSA 180

Query: 181 GDVRGMGGG----GMPSRSRSTEDIYTRSQLEASAANKDNFFAQKIAENDSRPEGIPPSQ 240
            D RG  GG    G P RS+ST+DIYTRSQLEASAANK+ FFAQKIAENDSRPEG+PPSQ
Sbjct: 181 SDFRGGAGGGGRSGAPVRSKSTQDIYTRSQLEASAANKETFFAQKIAENDSRPEGLPPSQ 240

Query: 241 GGKYVGFGSSPAPAR---RNDPQNDVFSVVSQGFGKLSLVAASAAQSAANAVQAGTKELT 300
           GGKYVGFGSSP P R    N+PQ DV S VSQG G+LS VAASAAQSAA+ VQAGTKE+T
Sbjct: 241 GGKYVGFGSSPVPPRMSNNNNPQGDVLSAVSQGLGRLSFVAASAAQSAASVVQAGTKEIT 300

Query: 301 TKVKEGGYDYKVNETVNVVTAKTTEIGQRTWGIMRGVMAMASQKVEEYAKDGMNWKNDGW 360
            KVKEGGYD+KVNETVNVVT KTTEIGQRTWGIM+GVMA+ASQKVEEY KDG++W N+ W
Sbjct: 301 AKVKEGGYDHKVNETVNVVTNKTTEIGQRTWGIMKGVMAIASQKVEEYTKDGVSWNNENW 360

Query: 361 QRNENEKNGYYQEFEHDNKGWNSSSGTGQSSGSGHHNNSYNSSSWDDWDTKDNRKEETTT 420
           QRN+NE+NGYYQEF+ +NKGWN+SSG GQSS SG +  +++S+SWDDWD KDN+KEETT 
Sbjct: 361 QRNDNERNGYYQEFKQENKGWNASSG-GQSSSSG-NVQTFSSTSWDDWDHKDNKKEETTK 420

Query: 421 KVSGTHNNNNNNNNNNNSNDGWAGWDDQKDDGYDHYYQASDRK-TVGQNGKAGGGTWSEG 472
              GT  N          NDGWAGWDD K+DG+D++YQ++  K T G NGK+   TW+EG
Sbjct: 421 --GGTSGN----------NDGWAGWDDAKEDGHDNWYQSAPNKGTAGHNGKS-DATWTEG 466

BLAST of Cucsa.175350 vs. NCBI nr
Match: gi|1009158824|ref|XP_015897488.1| (PREDICTED: ADP-ribosylation factor GTPase-activating protein AGD7-like [Ziziphus jujuba])

HSP 1 Score: 718.8 bits (1854), Expect = 6.3e-204
Identity = 364/482 (75.52%), Postives = 409/482 (84.85%), Query Frame = 1

Query: 1   MAASRRLRDLQSRPGNKICVDCSQKNPQWASVSYGVFMCLECSGKHRGLGVHISFVRSVT 60
           MAA+RRLRDLQS+PGNKICVDCSQKNPQWASVSYGVFMCLECSGKHRGLGVHISFVRSVT
Sbjct: 1   MAAARRLRDLQSQPGNKICVDCSQKNPQWASVSYGVFMCLECSGKHRGLGVHISFVRSVT 60

Query: 61  MDSWSEIQIKKMEAGGNEQLNAFLSQYGIPKETDIVTKYNTSAAGVYRDRIQALAEGRSW 120
           MDSWSEIQIKKME+GGNE+LN+FL+QYGIPKETDIVTKYNT+AA VYRDRIQALAEGR W
Sbjct: 61  MDSWSEIQIKKMESGGNERLNSFLAQYGIPKETDIVTKYNTNAASVYRDRIQALAEGRPW 120

Query: 121 KDPPAVKENIGVGKS-----RPPLAQSAGG-GGSKVNNGGWDSWDNEDNFRSSSDMRRNQ 180
           +DPP VKENI  G       +PPLA   GG  G+  NNGGWD+WDN+D FRSSSD+RRNQ
Sbjct: 121 RDPPVVKENISGGSGGGSNRKPPLAHGGGGRNGNFGNNGGWDNWDNDDGFRSSSDLRRNQ 180

Query: 181 STGDVRGMGGGG-MPSRSRSTEDIYTRSQLEASAANKDNFFAQKIAENDSRPEGIPPSQG 240
           STGDVRG+ GGG MP+RS+STEDIYTRSQLEASAANKD+FFA+K+AENDSRPEGIPPSQG
Sbjct: 181 STGDVRGVNGGGVMPARSKSTEDIYTRSQLEASAANKDSFFARKLAENDSRPEGIPPSQG 240

Query: 241 GKYVGFGSSPAPARRN---DPQNDVFSVVSQGFGKLSLVAASAAQSAANAVQAGTKELTT 300
           GKYVGFGS PAPA++    + Q D+FSVVSQGFG+LS+ AASAA SAAN VQ GT+E++T
Sbjct: 241 GKYVGFGSGPAPAQQRNNYNAQGDMFSVVSQGFGRLSVAAASAAASAANVVQQGTREIST 300

Query: 301 KVKEGGYDYKVNETVNVVTAKTTEIGQRTWGIMRGVMAMASQKVEEYAKDGMNWKNDGWQ 360
           KVKEGGYDYKVNETVNVV++KT+EIGQRTWGIM+GVMAMASQKVEEYAKDG  WK D WQ
Sbjct: 301 KVKEGGYDYKVNETVNVVSSKTSEIGQRTWGIMKGVMAMASQKVEEYAKDG--WKADNWQ 360

Query: 361 RNENEKNGYYQEFEHDNKGWNSSSGTGQSSGSGHHNNSYNSSSWDDWDTKDNRKEETTTK 420
           R+++EKNGYYQEF       NSSSG GQSS SG + NS++S+SWDDWD KDNR EE T  
Sbjct: 361 RDDSEKNGYYQEFNR-----NSSSGGGQSSSSG-NVNSFSSTSWDDWDQKDNRNEEVT-- 420

Query: 421 VSGTHNNNNNNNNNNNSNDGWAGWDDQKDDGYDHYYQ-ASDRKTVGQNGKAGGGTWSEGG 472
             GT +NN         +DGWAGWDD KDDGYD YYQ A+D+KTVG NGK+   TW+ GG
Sbjct: 421 -KGTASNN---------SDGWAGWDDAKDDGYDSYYQSATDKKTVGHNGKS-DATWTGGG 461

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
AGD6_ARATH3.9e-17765.90Probable ADP-ribosylation factor GTPase-activating protein AGD6 OS=Arabidopsis t... [more]
AGD7_ARATH1.2e-17565.83ADP-ribosylation factor GTPase-activating protein AGD7 OS=Arabidopsis thaliana G... [more]
ARFG1_MOUSE4.0e-3326.97ADP-ribosylation factor GTPase-activating protein 1 OS=Mus musculus GN=Arfgap1 P... [more]
ARFG1_HUMAN6.9e-3331.88ADP-ribosylation factor GTPase-activating protein 1 OS=Homo sapiens GN=ARFGAP1 P... [more]
ARFG1_RAT1.2e-3226.90ADP-ribosylation factor GTPase-activating protein 1 OS=Rattus norvegicus GN=Arfg... [more]
Match NameE-valueIdentityDescription
A0A0A0LAL4_CUCSA2.4e-27999.36Uncharacterized protein OS=Cucumis sativus GN=Csa_3G734360 PE=4 SV=1[more]
A0A061ET55_THECC1.6e-20675.73ARF-GAP domain 7 isoform 1 OS=Theobroma cacao GN=TCM_021978 PE=4 SV=1[more]
A0A059A6A6_EUCGR3.4e-20474.33Uncharacterized protein OS=Eucalyptus grandis GN=EUGRSUZ_K03154 PE=4 SV=1[more]
F6HLG9_VITVI1.7e-20374.22Putative uncharacterized protein OS=Vitis vinifera GN=VIT_08s0007g00010 PE=4 SV=... [more]
M5WAQ9_PRUPE2.9e-20374.29Uncharacterized protein OS=Prunus persica GN=PRUPE_ppa005179mg PE=4 SV=1[more]
Match NameE-valueIdentityDescription
AT3G53710.12.2e-17865.90 ARF-GAP domain 6[more]
AT2G37550.17.0e-17765.83 ARF-GAP domain 7[more]
AT4G17890.13.5e-2745.93 ARF-GAP domain 8[more]
AT5G46750.11.7e-2650.96 ARF-GAP domain 9[more]
AT2G35210.16.6e-2650.50 root and pollen arfgap[more]
Match NameE-valueIdentityDescription
gi|778683566|ref|XP_011651902.1|3.5e-27999.36PREDICTED: ADP-ribosylation factor GTPase-activating protein AGD7 [Cucumis sativ... [more]
gi|659083832|ref|XP_008442564.1|3.1e-27597.66PREDICTED: ADP-ribosylation factor GTPase-activating protein AGD7 [Cucumis melo][more]
gi|590629759|ref|XP_007027080.1|2.3e-20675.73ARF-GAP domain 7 isoform 1 [Theobroma cacao][more]
gi|702499008|ref|XP_010037851.1|4.8e-20474.33PREDICTED: probable ADP-ribosylation factor GTPase-activating protein AGD6 [Euca... [more]
gi|1009158824|ref|XP_015897488.1|6.3e-20475.52PREDICTED: ADP-ribosylation factor GTPase-activating protein AGD7-like [Ziziphus... [more]
The following terms have been associated with this gene:
Vocabulary: INTERPRO
TermDefinition
IPR001164ArfGAP_dom
Vocabulary: Molecular Function
TermDefinition
GO:0005096GTPase activator activity
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0043547 positive regulation of GTPase activity
biological_process GO:0008150 biological_process
cellular_component GO:0005575 cellular_component
molecular_function GO:0005096 GTPase activator activity
molecular_function GO:0046872 metal ion binding

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Cucsa.175350.1Cucsa.175350.1mRNA


Analysis Name: InterPro Annotations of cucumber (Gy14)
Date Performed: 2017-01-17
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001164Arf GTPase activating proteinPRINTSPR00405REVINTRACTNGcoord: 16..35
score: 1.2E-23coord: 35..52
score: 1.2E-23coord: 56..77
score: 1.2
IPR001164Arf GTPase activating proteinPFAMPF01412ArfGapcoord: 6..94
score: 1.6
IPR001164Arf GTPase activating proteinSMARTSM00105arf_gap_3coord: 1..120
score: 7.3
IPR001164Arf GTPase activating proteinPROFILEPS50115ARFGAPcoord: 4..120
score: 26
NoneNo IPR availablePANTHERPTHR23180CENTAURIN/ARFcoord: 1..457
score: 2.6E
NoneNo IPR availablePANTHERPTHR23180:SF293ADP-RIBOSYLATION FACTOR GTPASE-ACTIVATING PROTEIN AGD7coord: 1..457
score: 2.6E
NoneNo IPR availableunknownSSF57863ArfGap/RecO-like zinc fingercoord: 5..108
score: 8.63

The following gene(s) are paralogous to this gene:

None

The following block(s) are covering this gene:
GeneOrganismBlock
Cucsa.175350Melon (DHL92) v3.6.1cgymedB305
Cucsa.175350Melon (DHL92) v3.6.1cgymedB306
Cucsa.175350Melon (DHL92) v3.6.1cgymedB313
Cucsa.175350Silver-seed gourdcarcgyB0398
Cucsa.175350Silver-seed gourdcarcgyB1051
Cucsa.175350Cucumber (Chinese Long) v3cgycucB283
Cucsa.175350Cucumber (Chinese Long) v3cgycucB284
Cucsa.175350Cucumber (Chinese Long) v3cgycucB286
Cucsa.175350Watermelon (97103) v2cgywmbB319
Cucsa.175350Watermelon (97103) v2cgywmbB321
Cucsa.175350Watermelon (97103) v2cgywmbB322
Cucsa.175350Wax gourdcgywgoB362
Cucsa.175350Cucumber (Gy14) v1cgycgyB016
Cucsa.175350Cucumber (Gy14) v1cgycgyB105
Cucsa.175350Cucurbita maxima (Rimu)cgycmaB0501
Cucsa.175350Cucurbita maxima (Rimu)cgycmaB0502
Cucsa.175350Cucurbita maxima (Rimu)cgycmaB0503
Cucsa.175350Cucurbita moschata (Rifu)cgycmoB0503
Cucsa.175350Cucurbita moschata (Rifu)cgycmoB0504
Cucsa.175350Cucurbita moschata (Rifu)cgycmoB0508
Cucsa.175350Wild cucumber (PI 183967)cgycpiB279
Cucsa.175350Wild cucumber (PI 183967)cgycpiB281
Cucsa.175350Wild cucumber (PI 183967)cgycpiB283
Cucsa.175350Cucumber (Chinese Long) v2cgycuB269
Cucsa.175350Cucumber (Chinese Long) v2cgycuB271
Cucsa.175350Cucumber (Chinese Long) v2cgycuB273
Cucsa.175350Melon (DHL92) v3.5.1cgymeB311
Cucsa.175350Melon (DHL92) v3.5.1cgymeB312
Cucsa.175350Melon (DHL92) v3.5.1cgymeB318
Cucsa.175350Watermelon (Charleston Gray)cgywcgB314
Cucsa.175350Watermelon (Charleston Gray)cgywcgB316
Cucsa.175350Watermelon (Charleston Gray)cgywcgB318
Cucsa.175350Watermelon (97103) v1cgywmB337
Cucsa.175350Watermelon (97103) v1cgywmB338
Cucsa.175350Watermelon (97103) v1cgywmB339
Cucsa.175350Cucurbita pepo (Zucchini)cgycpeB0482
Cucsa.175350Cucurbita pepo (Zucchini)cgycpeB0483
Cucsa.175350Cucurbita pepo (Zucchini)cgycpeB0486
Cucsa.175350Bottle gourd (USVL1VR-Ls)cgylsiB291
Cucsa.175350Bottle gourd (USVL1VR-Ls)cgylsiB294
Cucsa.175350Bottle gourd (USVL1VR-Ls)cgylsiB299