Cucsa.172950 (gene) Cucumber (Gy14) v1

NameCucsa.172950
Typegene
OrganismCucumis sativus (Cucumber (Gy14) v1)
DescriptionAspartic proteinase nepenthesin-1, putative
Locationscaffold01201 : 115020 .. 116580 (+)
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGCTAGTCGTTGAACAACAGGGGAGCTATTTGATGAAATTGTCATTAGGGATGCCTCCTGTTTCATTCTCTGCCATTTTTTATATTGGCAGCGACTTGGTATGGACGCAATGCAAGCCATGCTTTGAGCAGCCAACTTCTGTATATGATCCTGCACAATTGTCTTCCTTTGCCAATGTTATTGCCAATGTTACTCGTACTCACTCCCCTACATTAGGCTAGAAATTGACCAATTCAAATTCTAGTAATGGTTCAAATTCCAGTAaTGGTTGTGAGCTAAGAACCACAGATACTTCATATTAGAGTTGGTATCTATGTCGGACACTTAACGGACACGAATTCGTCTGGACACGTTGCGGACACGTGTCGGACACGCCAAAATTTGTGTCCTTTTTTtAATTGTCCGCACAGGTCTTAGACACATCCGGACACTCCATTTTCCTTTTTGGAAGAAGCCCAAAGCCCAGACCAGCCCAGACCAGCCCACTAAACTAATTCTAATAAATAATATTTAACTTCCAGCCCAAAGCCCAGTCCACTGAACTAGTTCTAATAAATAATCAAATATAAAGATTTTATTAGATAAACATACAGTAAATCAAATCTTCTTCAGTTTTCTTCCTTTCTCTTCCGTGGAGGAGGGCACGTTTTCTTTTGTGGAGGAAAGTTTTTTTtATTAGTATTTAATATTTTATTCTTTTGTATGAAAATCACTGTTGAATATACCTAATATGTTTTGTACTATTACAACCTATAATAGTTAAATGTTAATTTGACATTTTATGTTTTTTTAGTGAAGATTGAAGAACATTTAGTTTTTTTTTTTtAGGAAATGAAAAACATTTAGTGTTTTGTATCAAATTTATATATATTATATAAAAAAaTACTTTAAAAAAaTAACGTATCCCCAACGTGTCCGTGTCCTATTATTTTAGAAATTGACGTATCGTCGTGTCCTATCGTATCTATGTATGTGTCTGTGCTTCTTAGGTTGGAGTATTCATATAGTTATAGAGACGGGTCTTTCTCTATAGGTTTTTTGGCATTCGAGACCTTGACCCTTGGGGAAGCAAATCAGCAAGTTTATACACAAGATATAGTGTTTGGGGCGAGATAAAGAACCATGTAAAGGGCTTGACACAGGGTGCTGGCATAGATAGGTTCAACATAGGACCGGTATTGAACTGGGAGTCTGGCCTTTGAATCATCTTCAAAACACATTGTCAAACCCACGAACCGAGCAATCAAGACCACGCCACTAATACAAAATCCATTTGATCCATCTTACTACTATCTATCTCTACAAGCTGTCCCGTCCTCATGGTTTAAACTAAACAATGATGGCACCAGCGGCGTGAACATAGATTCGAGCACATCGATCATGTACTTCAGAGGATGCCTTTGGTCAGCAAGTATTGGGCTTGACCTCTGCTTTGAGCTCCCTTCCCACAAAAATAGTAAACTTGATGTGCCTGATTTGATATTTCATTTTGAAGGTCTTGACTTGAAGCTTCCTGTTGATAACTATATGGTCGTCGACGAGGAGCTAGGGATTACATGA

mRNA sequence

ATGCTAGTCGTTGAACAACAGGGGAGCTATTTGATGAAATTGTCATTAGGGATGCCTCCTGTTTCATTCTCTGCCATTTTTTATATTGGCAGCGACTTGGTATGGACGCAATGCAAGCCATGCTTTGAGCAGCCAACTTCTGTATATGATCCTGCACAATTGTCTTCCTTTGCCAATGTTATTGCCAATGTTACTCaaattgacgtatcgtcgtgtcctatcgtatctatgtatgtgtctgtgcttcttaggttgGAGTATTCATATAGTTATAGAGACGGGTCTTTCTCTATAGGTTTTTTGGCATTCGAGACCTTGACCCTTGGGGAAGCAAATCAGCAAGTTTATACACAAGATATAGGCTTGACACAGGGTGCTGGCATAGATAGGTTCAACATAGGACCGACCACGCCACTAATACAAAATCCATTTGATCCATCTTACTACTATCTATCTCTACAAGCTGTCCCGTCCTCATGGTTTAAACTAAACAATGATGGCACCAGCGGCGTGAACATAGATTCGAGCACATCGATCATGTACTTCAGAGGATGCCTTTGGTCAGCAAGTATTGGGCTTGACCTCTGCTTTGAGCTCCCTTCCCACAAAAATAGTAAACTTGATGTGCCTGATTTGATATTTCATTTTGAAGGTCTTGACTTGAAGCTTCCTGTTGATAACTATATGGTCGTCGACGAGGAGCTAGGGATTACATGA

Coding sequence (CDS)

ATGCTAGTCGTTGAACAACAGGGGAGCTATTTGATGAAATTGTCATTAGGGATGCCTCCTGTTTCATTCTCTGCCATTTTTTATATTGGCAGCGACTTGGTATGGACGCAATGCAAGCCATGCTTTGAGCAGCCAACTTCTGTATATGATCCTGCACAATTGTCTTCCTTTGCCAATGTTATTGCCAATGTTACTCAAATTGACGTATCGTCGTGTCCTATCGTATCTATGTATGTGTCTGTGCTTCTTAGGTTGGAGTATTCATATAGTTATAGAGACGGGTCTTTCTCTATAGGTTTTTTGGCATTCGAGACCTTGACCCTTGGGGAAGCAAATCAGCAAGTTTATACACAAGATATAGGCTTGACACAGGGTGCTGGCATAGATAGGTTCAACATAGGACCGACCACGCCACTAATACAAAATCCATTTGATCCATCTTACTACTATCTATCTCTACAAGCTGTCCCGTCCTCATGGTTTAAACTAAACAATGATGGCACCAGCGGCGTGAACATAGATTCGAGCACATCGATCATGTACTTCAGAGGATGCCTTTGGTCAGCAAGTATTGGGCTTGACCTCTGCTTTGAGCTCCCTTCCCACAAAAATAGTAAACTTGATGTGCCTGATTTGATATTTCATTTTGAAGGTCTTGACTTGAAGCTTCCTGTTGATAACTATATGGTCGTCGACGAGGAGCTAGGGATTACATGA

Protein sequence

MLVVEQQGSYLMKLSLGMPPVSFSAIFYIGSDLVWTQCKPCFEQPTSVYDPAQLSSFANVIANVTQIDVSSCPIVSMYVSVLLRLEYSYSYRDGSFSIGFLAFETLTLGEANQQVYTQDIGLTQGAGIDRFNIGPTTPLIQNPFDPSYYYLSLQAVPSSWFKLNNDGTSGVNIDSSTSIMYFRGCLWSASIGLDLCFELPSHKNSKLDVPDLIFHFEGLDLKLPVDNYMVVDEELGIT*
BLAST of Cucsa.172950 vs. Swiss-Prot
Match: NEP1_NEPGR (Aspartic proteinase nepenthesin-1 OS=Nepenthes gracilis GN=nep1 PE=1 SV=1)

HSP 1 Score: 81.6 bits (200), Expect = 1.2e-14
Identity = 52/138 (37.68%), Postives = 67/138 (48.55%), Query Frame = 1

Query: 8   GSYLMKLSLGMPPVSFSAIFYIGSDLVWTQCKP---CFEQPTSVYDPAQLSSFANVIANV 67
           G YLM LS+G P   FSAI   GSDL+WTQC+P   CF Q T +++P   SSF+ +  + 
Sbjct: 93  GEYLMNLSIGTPAQPFSAIMDTGSDLIWTQCQPCTQCFNQSTPIFNPQGSSSFSTLPCSS 152

Query: 68  TQIDVSSCPIVSMYVSVLLRLEYSYSYRDGSFSIGFLAFETLTLGEANQQVYT------- 127
                 S P  S         +Y+Y Y DGS + G +  ETLT G  +    T       
Sbjct: 153 QLCQALSSPTCSNNF-----CQYTYGYGDGSETQGSMGTETLTFGSVSIPNITFGCGENN 212

Query: 128 QDIGLTQGAGIDRFNIGP 136
           Q  G   GAG+     GP
Sbjct: 213 QGFGQGNGAGLVGMGRGP 225


HSP 2 Score: 70.1 bits (170), Expect = 3.7e-11
Identity = 41/123 (33.33%), Postives = 63/123 (51.22%), Query Frame = 1

Query: 135 PTTPLIQNPFDPSYYYLSLQAV----------PSSWFKLNNDGTSGVNIDSSTSIMYFRG 194
           P T LIQ+   P++YY++L  +          PS++   +N+GT G+ IDS T++ YF  
Sbjct: 266 PNTTLIQSSQIPTFYYITLNGLSVGSTRLPIDPSAFALNSNNGTGGIIIDSGTTLTYFVN 325

Query: 195 CLWSA-----------------SIGLDLCFELPSHKNSKLDVPDLIFHFEGLDLKLPVDN 231
             + +                 S G DLCF+ PS   S L +P  + HF+G DL+LP +N
Sbjct: 326 NAYQSVRQEFISQINLPVVNGSSSGFDLCFQTPSDP-SNLQIPTFVMHFDGGDLELPSEN 385

BLAST of Cucsa.172950 vs. Swiss-Prot
Match: NEP2_NEPGR (Aspartic proteinase nepenthesin-2 OS=Nepenthes gracilis GN=nep2 PE=1 SV=1)

HSP 1 Score: 80.5 bits (197), Expect = 2.8e-14
Identity = 55/150 (36.67%), Postives = 70/150 (46.67%), Query Frame = 1

Query: 8   GSYLMKLSLGMPPVSFSAIFYIGSDLVWTQCKP---CFEQPTSVYDPAQLSSFANVIANV 67
           G YLM +++G P  SFSAI   GSDL+WTQC+P   CF QPT +++P   SSF       
Sbjct: 94  GEYLMNVAIGTPDSSFSAIMDTGSDLIWTQCEPCTQCFSQPTPIFNPQDSSSF------- 153

Query: 68  TQIDVSSCPIVSMYVSVL-------LRLEYSYSYRDGSFSIGFLAFETLTL--------- 127
                S+ P  S Y   L          +Y+Y Y DGS + G++A ET T          
Sbjct: 154 -----STLPCESQYCQDLPSETCNNNECQYTYGYGDGSTTQGYMATETFTFETSSVPNIA 213

Query: 128 ---GEANQQVYTQDIGLTQGAGIDRFNIGP 136
              GE N     Q  G   GAG+     GP
Sbjct: 214 FGCGEDN-----QGFGQGNGAGLIGMGWGP 226


HSP 2 Score: 63.9 bits (154), Expect = 2.7e-09
Identity = 50/164 (30.49%), Postives = 75/164 (45.73%), Query Frame = 1

Query: 97  SIGFLAFETLTLGEANQQVYTQDIGLTQGAGIDRFNIGPTTPLIQNPFDPSYYYLSLQA- 156
           S G  +  TL LG A         G+ +G+        P+T LI +  +P+YYY++LQ  
Sbjct: 244 SYGSSSPSTLALGSAAS-------GVPEGS--------PSTTLIHSSLNPTYYYITLQGI 303

Query: 157 --------VPSSWFKLNNDGTSGVNIDSSTSIMYFRGCLWSA-----------------S 216
                   +PSS F+L +DGT G+ IDS T++ Y     ++A                 S
Sbjct: 304 TVGGDNLGIPSSTFQLQDDGTGGMIIDSGTTLTYLPQDAYNAVAQAFTDQINLPTVDESS 363

Query: 217 IGLDLCFELPSHKNSKLDVPDLIFHFEGLDLKLPVDNYMVVDEE 235
            GL  CF+ PS   S + VP++   F+G  L L   N ++   E
Sbjct: 364 SGLSTCFQQPS-DGSTVQVPEISMQFDGGVLNLGEQNILISPAE 391

BLAST of Cucsa.172950 vs. Swiss-Prot
Match: ASPA_ARATH (Aspartyl protease family protein At5g10770 OS=Arabidopsis thaliana GN=At5g10770 PE=2 SV=1)

HSP 1 Score: 72.4 bits (176), Expect = 7.5e-12
Identity = 49/152 (32.24%), Postives = 70/152 (46.05%), Query Frame = 1

Query: 8   GSYLMKLSLGMPPVSFSAIFYIGSDLVWTQCKP----CFEQPTSVYDPAQLSSFANVIAN 67
           G+Y++ + LG P    S IF  GSDL WTQC+P    C++Q   +++P++ +S+ NV  +
Sbjct: 130 GNYIVTVGLGTPKNDLSLIFDTGSDLTWTQCQPCVRTCYDQKEPIFNPSKSTSYYNVSCS 189

Query: 68  VTQIDVSSCPIVSMYVSVLLRLEYSYSYRDGSFSIGFLAFETLTL-------------GE 127
                  S    +          Y   Y D SFS+GFLA E  TL             GE
Sbjct: 190 SAACGSLSSATGNAGSCSASNCIYGIQYGDQSFSVGFLAKEKFTLTNSDVFDGVYFGCGE 249

Query: 128 ANQQVYTQDIGLTQGAGIDRFNIGPTTPLIQN 143
            NQ ++T   GL  G G D+ +    T    N
Sbjct: 250 NNQGLFTGVAGLL-GLGRDKLSFPSQTATAYN 280

BLAST of Cucsa.172950 vs. Swiss-Prot
Match: CDR1_ARATH (Aspartic proteinase CDR1 OS=Arabidopsis thaliana GN=CDR1 PE=1 SV=1)

HSP 1 Score: 70.5 bits (171), Expect = 2.9e-11
Identity = 41/116 (35.34%), Postives = 60/116 (51.72%), Query Frame = 1

Query: 3   VVEQQGSYLMKLSLGMPPVSFSAIFYIGSDLVWTQCKP---CFEQPTSVYDPAQLSSFAN 62
           +    G YLM +S+G PP    AI   GSDL+WTQC P   C+ Q   ++DP   S++ +
Sbjct: 83  LTSNSGEYLMNVSIGTPPFPIMAIADTGSDLLWTQCAPCDDCYTQVDPLFDPKTSSTYKD 142

Query: 63  VIANVTQIDVSSCPIVSMYVSVLLR---LEYSYSYRDGSFSIGFLAFETLTLGEAN 113
           V  + +Q     C  +    S         YS SY D S++ G +A +TLTLG ++
Sbjct: 143 VSCSSSQ-----CTALENQASCSTNDNTCSYSLSYGDNSYTKGNIAVDTLTLGSSD 193


HSP 2 Score: 37.0 bits (84), Expect = 3.5e-01
Identity = 33/124 (26.61%), Postives = 55/124 (44.35%), Query Frame = 1

Query: 136 TTPLIQNPFDPSYYYLSLQAVPSSWFKLNNDGTSG------VNIDSSTSIMYFRGCLWS- 195
           +TPLI      ++YYL+L+++     ++   G+        + IDS T++       +S 
Sbjct: 275 STPLIAKASQETFYYLTLKSISVGSKQIQYSGSDSESSEGNIIIDSGTTLTLLPTEFYSE 334

Query: 196 ------ASI----------GLDLCFELPSHKNSKLDVPDLIFHFEGLDLKLPVDN-YMVV 236
                 +SI          GL LC+         L VP +  HF+G D+KL   N ++ V
Sbjct: 335 LEDAVASSIDAEKKQDPQSGLSLCYSA----TGDLKVPVITMHFDGADVKLDSSNAFVQV 394

BLAST of Cucsa.172950 vs. Swiss-Prot
Match: AED1_ARATH (Aspartyl protease AED1 OS=Arabidopsis thaliana GN=AED1 PE=2 SV=1)

HSP 1 Score: 63.9 bits (154), Expect = 2.7e-09
Identity = 47/153 (30.72%), Postives = 68/153 (44.44%), Query Frame = 1

Query: 8   GSYLMKLSLGMPPVSFSAIFYIGSDLVWTQCKP----CFEQPTSVYDPAQLSSFANVIAN 67
           G+Y++ + +G P    S +F  GSDL WTQC+P    C+ Q    ++P+  S++ NV  +
Sbjct: 130 GNYIVTIGIGTPKHDLSLVFDTGSDLTWTQCEPCLGSCYSQKEPKFNPSSSSTYQNVSCS 189

Query: 68  VTQI-DVSSCPIVSMYVSVLLRLEYSYSYRDGSFSIGFLAFETLTL-------------G 127
                D  SC   +          YS  Y D SF+ GFLA E  TL             G
Sbjct: 190 SPMCEDAESCSASNCV--------YSIVYGDKSFTQGFLAKEKFTLTNSDVLEDVYFGCG 249

Query: 128 EANQQVYTQDIGLTQGAGIDRFNIGPTTPLIQN 143
           E NQ ++    GL  G G  + ++   T    N
Sbjct: 250 ENNQGLFDGVAGLL-GLGPGKLSLPAQTTTTYN 273

BLAST of Cucsa.172950 vs. TrEMBL
Match: M0U7H4_MUSAM (Uncharacterized protein OS=Musa acuminata subsp. malaccensis PE=3 SV=1)

HSP 1 Score: 164.9 bits (416), Expect = 1.2e-37
Identity = 104/286 (36.36%), Postives = 141/286 (49.30%), Query Frame = 1

Query: 8   GSYLMKLSLGMPPVSFSAIFYIGSDLVWTQCKP---CFEQPTSVYDPAQLSSFANVIANV 67
           G +LM L++G P ++F AI   GSDL+WTQCKP   CF QPT V+DP+  S++ N+    
Sbjct: 85  GEFLMDLAIGTPSLAFPAIIDTGSDLIWTQCKPCDECFSQPTPVFDPSSSSTYTNL---- 144

Query: 68  TQIDVSSCPIVSMYVSVLLRLEYSYSYRDGSFSIGFLAFETLTLGEANQQVYT------- 127
                + C  +  +       EY YSY D S + G LA ET T G  N    +       
Sbjct: 145 -PCSSNLCQALPTFTCGASSCEYLYSYGDSSSTQGVLASETFTFGTENSTAVSGVAFGCG 204

Query: 128 ---QDIGLTQGAGIDRFNIGP------------------TTPLIQNPFDPSYYYLSLQ-- 187
              Q  G +QG+G+     GP                  +TPL+QNP  P+ YYLSL+  
Sbjct: 205 DTNQGSGFSQGSGLVGLGRGPLSLISQLDLGNAAASAIQSTPLVQNPKHPALYYLSLKDI 264

Query: 188 -------AVPSSWFKLNNDGTSGVNIDSSTSIMYFRGCLW-----------------SAS 237
                   +PSS F +  DG+ G+ IDS TSI Y     +                  + 
Sbjct: 265 SVGGNRLQIPSSTFAVQEDGSGGLIIDSGTSITYLEVGAYRRLKKAFLSQMQLPVADGSE 324

BLAST of Cucsa.172950 vs. TrEMBL
Match: F6H4G8_VITVI (Putative uncharacterized protein OS=Vitis vinifera GN=VIT_07s0031g02900 PE=3 SV=1)

HSP 1 Score: 161.8 bits (408), Expect = 1.1e-36
Identity = 110/309 (35.60%), Postives = 146/309 (47.25%), Query Frame = 1

Query: 8   GSYLMKLSLGMPPVSFSAIFYIGSDLVWTQCKPC---FEQPTSVYDPAQLSSFANVIAN- 67
           G +LMKL++G P  ++SAI   GSDL+WTQCKPC   F+QPT ++DP + SSF+ +  + 
Sbjct: 88  GEFLMKLAIGTPAETYSAIMDTGSDLIWTQCKPCKDCFDQPTPIFDPKKSSSFSKLPCSS 147

Query: 68  --VTQIDVSSCPIVSMYVSVLLRLEYSYSYRDGSFSIGFLAFETLTLGEANQQ------- 127
                + +SSC             EY YSY D S + G LA ET   G+A+         
Sbjct: 148 DLCAALPISSCSD---------GCEYLYSYGDYSSTQGVLATETFAFGDASVSKIGFGCG 207

Query: 128 ------VYTQDIGLT--------------------------QGAGIDRFNIGP------- 187
                  ++Q  GL                              GI    +G        
Sbjct: 208 EDNDGSGFSQGAGLVGLGRGPLSLISQLGEPKFSYCLTSMDDSKGISSLLVGSEATMKNA 267

Query: 188 -TTPLIQNPFDPSYYYLSLQAVP---------SSWFKLNNDGTSGVNIDSSTSIMYFRGC 238
            TTPLIQNP  PS+YYLSL+ +           S F + NDG+ G+ IDS T+I Y    
Sbjct: 268 ITTPLIQNPSQPSFYYLSLEGISVGDTLLPIEKSTFSIQNDGSGGLIIDSGTTITYLEDS 327

BLAST of Cucsa.172950 vs. TrEMBL
Match: A0A0D9W5K9_9ORYZ (Uncharacterized protein OS=Leersia perrieri PE=3 SV=1)

HSP 1 Score: 155.2 bits (391), Expect = 9.9e-35
Identity = 110/306 (35.95%), Postives = 158/306 (51.63%), Query Frame = 1

Query: 8   GSYLMKLSLGMPPVSFSAIFYIGSDLVWTQCKPC---FEQPTSVYDPAQLSSFANV---I 67
           G +LM LS+G P V++SAI   GSDLVWTQCKPC   F+QPT V+DP+  S++A V    
Sbjct: 103 GEFLMDLSIGTPAVAYSAIVDTGSDLVWTQCKPCVDCFKQPTPVFDPSSSSTYAAVPCSS 162

Query: 68  ANVTQIDVSSCPIVSMYVSVLLRLEYSYSYRDGSFSIGFLAFETLTL------------G 127
           A+ + +  S+C   S       R  Y+Y+Y D S + G L  ET TL            G
Sbjct: 163 ASCSDLPTSTCTKAS-------RCGYTYTYGDSSSTQGVLGTETFTLSKSKLPGVVFGCG 222

Query: 128 EANQ-QVYTQD---IGLTQGA-------GIDRFN---------------IGP-------- 187
           + N+   ++Q    +GL +G        G+++F+               +G         
Sbjct: 223 DTNEGDGFSQGAGLVGLGRGPLSLVAQLGLEKFSYCLTSLDDTKKSPLLLGSLAEISAKS 282

Query: 188 --TTPLIQNPFDPSYYYLSLQAV---------PSSWFKLNNDGTSGVNIDSSTSIMYFRG 233
             TTPLI+NP  PS+YY++L+A+         P+S F + +DGT GV +DS TSI Y   
Sbjct: 283 VQTTPLIKNPTQPSFYYVTLKAITVGSTWITLPASAFAVQDDGTGGVIVDSGTSITYLEV 342

BLAST of Cucsa.172950 vs. TrEMBL
Match: F6HZI3_VITVI (Putative uncharacterized protein OS=Vitis vinifera GN=VIT_07s0005g03140 PE=3 SV=1)

HSP 1 Score: 153.7 bits (387), Expect = 2.9e-34
Identity = 106/309 (34.30%), Postives = 148/309 (47.90%), Query Frame = 1

Query: 8   GSYLMKLSLGMPPVSFSAIFYIGSDLVWTQCKPC---FEQPTSVYDPAQLSSFANVIAN- 67
           G +LM L++G P  ++SAI   GSDL+WTQCKPC   F+QPT ++DP + SSF+ +  + 
Sbjct: 95  GEFLMNLAIGTPAETYSAIMDTGSDLIWTQCKPCKVCFDQPTPIFDPEKSSSFSKLPCSS 154

Query: 68  --VTQIDVSSCPIVSMYVSVLLRLEYSYSYRDGSFSIGFLAFETLTLGEANQ-------- 127
                + +SSC             EY YSY D S + G LA ET T G+A+         
Sbjct: 155 DLCVALPISSCSD---------GCEYRYSYGDHSSTQGVLATETFTFGDASVSKIGFGCG 214

Query: 128 -----QVYTQDIGLT----------QGAGIDRFNIGPT---------------------- 187
                + Y+Q  GL              G+ +F+   T                      
Sbjct: 215 EDNRGRAYSQGAGLVGLGRGPLSLISQLGVPKFSYCLTSIDDSKGISTLLVGSEATVKSA 274

Query: 188 --TPLIQNPFDPSYYYLSLQAVP---------SSWFKLNNDGTSGVNIDSSTSIMYFRGC 238
             TPLIQNP  PS+YYLSL+ +           S F + +DG+ G+ IDS T+I Y +  
Sbjct: 275 IPTPLIQNPSRPSFYYLSLEGISVGDTLLPIEKSTFSIQDDGSGGLIIDSGTTITYLKDS 334

BLAST of Cucsa.172950 vs. TrEMBL
Match: A5BUH7_VITVI (Putative uncharacterized protein OS=Vitis vinifera GN=VITISV_006338 PE=3 SV=1)

HSP 1 Score: 151.4 bits (381), Expect = 1.4e-33
Identity = 105/309 (33.98%), Postives = 148/309 (47.90%), Query Frame = 1

Query: 8   GSYLMKLSLGMPPVSFSAIFYIGSDLVWTQCKPC---FEQPTSVYDPAQLSSFANVIAN- 67
           G +LM L++G P  ++SAI   GSDL+WTQCKPC   F+QPT ++DP + SSF+ +  + 
Sbjct: 95  GEFLMNLAIGTPAETYSAIMDTGSDLIWTQCKPCKVCFDQPTPIFDPEKSSSFSKLPCSS 154

Query: 68  --VTQIDVSSCPIVSMYVSVLLRLEYSYSYRDGSFSIGFLAFETLTLGEANQ-------- 127
                + +SSC             EY YSY D S + G LA ET T G+A+         
Sbjct: 155 DLCVALPISSCSD---------GCEYRYSYGDHSSTQGVLATETFTFGDASVSKIGFGCG 214

Query: 128 -----QVYTQDIGLT----------QGAGIDRFNIGPT---------------------- 187
                + Y+Q  GL              G+ +F+   T                      
Sbjct: 215 EDNRGRAYSQGAGLVGLGRGPLSLISQLGVPKFSYCLTSIDDSKGISTLLVGSEATVKSA 274

Query: 188 --TPLIQNPFDPSYYYLSLQAVP---------SSWFKLNNDGTSGVNIDSSTSIMYFRGC 238
             TPLIQNP  PS+YYLSL+ +           S F + +DG+ G+ IDS T+I Y +  
Sbjct: 275 IPTPLIQNPSRPSFYYLSLEGISVGDTLLPIEKSTFSIQDDGSGGLIIDSGTTITYLKDN 334

BLAST of Cucsa.172950 vs. TAIR10
Match: AT2G03200.1 (AT2G03200.1 Eukaryotic aspartyl protease family protein)

HSP 1 Score: 89.0 bits (219), Expect = 4.4e-18
Identity = 55/139 (39.57%), Postives = 73/139 (52.52%), Query Frame = 1

Query: 125 GAGIDRFNIGPTTPLIQNPFDPSYYYLSLQ---------AVPSSWFKLNNDGTSGVNIDS 184
           GA +D   +  T  L++NP  PS+YYL LQ         +V  S F+L  DGT G+ IDS
Sbjct: 282 GASLDG-EVTKTMSLLRNPDQPSFYYLELQGITVGAKRLSVEKSTFELAEDGTGGMIIDS 341

Query: 185 STSIMYFRGCLW-----------------SASIGLDLCFELPSHKNSKLDVPDLIFHFEG 238
            T+I Y     +                 S S GLDLCF+LP    + + VP +IFHF+G
Sbjct: 342 GTTITYLEETAFKVLKEEFTSRMSLPVDDSGSTGLDLCFKLPDAAKN-IAVPKMIFHFKG 401


HSP 2 Score: 83.2 bits (204), Expect = 2.4e-16
Identity = 54/148 (36.49%), Postives = 77/148 (52.03%), Query Frame = 1

Query: 8   GSYLMKLSLGMPPVSFSAIFYIGSDLVWTQCKP---CFEQPTSVYDPAQLSSFANVIAN- 67
           G +LM+LS+G P V +SAI   GSDL+WTQCKP   CF+QPT ++DP + SS++ V  + 
Sbjct: 105 GEFLMELSIGNPAVKYSAIVDTGSDLIWTQCKPCTECFDQPTPIFDPEKSSSYSKVGCSS 164

Query: 68  --VTQIDVSSCPIVSMYVSVLLRLEYSYSYRDGSFSIGFLAFETLTLGEANQ-------- 127
                +  S+C             EY Y+Y D S + G LA ET T  + N         
Sbjct: 165 GLCNALPRSNCN------EDKDACEYLYTYGDYSSTRGLLATETFTFEDENSISGIGFGC 224

Query: 128 QVYTQDIGLTQGAGIDRFNIGPTTPLIQ 142
            V  +  G +QG+G+     GP + + Q
Sbjct: 225 GVENEGDGFSQGSGLVGLGRGPLSLISQ 246

BLAST of Cucsa.172950 vs. TAIR10
Match: AT1G64830.1 (AT1G64830.1 Eukaryotic aspartyl protease family protein)

HSP 1 Score: 75.1 bits (183), Expect = 6.6e-14
Identity = 39/118 (33.05%), Postives = 68/118 (57.63%), Query Frame = 1

Query: 3   VVEQQGSYLMKLSLGMPPVSFSAIFYIGSDLVWTQCKP---CFEQPTSVYDPAQLSSFAN 62
           +   +G YLM +S+G PPV   AI   GSDL+WTQC P   C++Q + ++DP + S++  
Sbjct: 79  ITSNRGEYLMNISIGTPPVPILAIADTGSDLIWTQCNPCEDCYQQTSPLFDPKESSTYRK 138

Query: 63  VIANVTQ---IDVSSCPIVSMYVSVLLRLEYSYSYRDGSFSIGFLAFETLTLGEANQQ 115
           V  + +Q   ++ +SC       S      Y+ +Y D S++ G +A +T+T+G + ++
Sbjct: 139 VSCSSSQCRALEDASCSTDENTCS------YTITYGDNSYTKGDVAVDTVTMGSSGRR 190

BLAST of Cucsa.172950 vs. TAIR10
Match: AT5G10770.1 (AT5G10770.1 Eukaryotic aspartyl protease family protein)

HSP 1 Score: 72.4 bits (176), Expect = 4.3e-13
Identity = 49/152 (32.24%), Postives = 70/152 (46.05%), Query Frame = 1

Query: 8   GSYLMKLSLGMPPVSFSAIFYIGSDLVWTQCKP----CFEQPTSVYDPAQLSSFANVIAN 67
           G+Y++ + LG P    S IF  GSDL WTQC+P    C++Q   +++P++ +S+ NV  +
Sbjct: 130 GNYIVTVGLGTPKNDLSLIFDTGSDLTWTQCQPCVRTCYDQKEPIFNPSKSTSYYNVSCS 189

Query: 68  VTQIDVSSCPIVSMYVSVLLRLEYSYSYRDGSFSIGFLAFETLTL-------------GE 127
                  S    +          Y   Y D SFS+GFLA E  TL             GE
Sbjct: 190 SAACGSLSSATGNAGSCSASNCIYGIQYGDQSFSVGFLAKEKFTLTNSDVFDGVYFGCGE 249

Query: 128 ANQQVYTQDIGLTQGAGIDRFNIGPTTPLIQN 143
            NQ ++T   GL  G G D+ +    T    N
Sbjct: 250 NNQGLFTGVAGLL-GLGRDKLSFPSQTATAYN 280

BLAST of Cucsa.172950 vs. TAIR10
Match: AT5G33340.1 (AT5G33340.1 Eukaryotic aspartyl protease family protein)

HSP 1 Score: 70.5 bits (171), Expect = 1.6e-12
Identity = 41/116 (35.34%), Postives = 60/116 (51.72%), Query Frame = 1

Query: 3   VVEQQGSYLMKLSLGMPPVSFSAIFYIGSDLVWTQCKP---CFEQPTSVYDPAQLSSFAN 62
           +    G YLM +S+G PP    AI   GSDL+WTQC P   C+ Q   ++DP   S++ +
Sbjct: 83  LTSNSGEYLMNVSIGTPPFPIMAIADTGSDLLWTQCAPCDDCYTQVDPLFDPKTSSTYKD 142

Query: 63  VIANVTQIDVSSCPIVSMYVSVLLR---LEYSYSYRDGSFSIGFLAFETLTLGEAN 113
           V  + +Q     C  +    S         YS SY D S++ G +A +TLTLG ++
Sbjct: 143 VSCSSSQ-----CTALENQASCSTNDNTCSYSLSYGDNSYTKGNIAVDTLTLGSSD 193


HSP 2 Score: 37.0 bits (84), Expect = 2.0e-02
Identity = 33/124 (26.61%), Postives = 55/124 (44.35%), Query Frame = 1

Query: 136 TTPLIQNPFDPSYYYLSLQAVPSSWFKLNNDGTSG------VNIDSSTSIMYFRGCLWS- 195
           +TPLI      ++YYL+L+++     ++   G+        + IDS T++       +S 
Sbjct: 275 STPLIAKASQETFYYLTLKSISVGSKQIQYSGSDSESSEGNIIIDSGTTLTLLPTEFYSE 334

Query: 196 ------ASI----------GLDLCFELPSHKNSKLDVPDLIFHFEGLDLKLPVDN-YMVV 236
                 +SI          GL LC+         L VP +  HF+G D+KL   N ++ V
Sbjct: 335 LEDAVASSIDAEKKQDPQSGLSLCYSA----TGDLKVPVITMHFDGADVKLDSSNAFVQV 394

BLAST of Cucsa.172950 vs. TAIR10
Match: AT2G28010.1 (AT2G28010.1 Eukaryotic aspartyl protease family protein)

HSP 1 Score: 67.4 bits (163), Expect = 1.4e-11
Identity = 38/102 (37.25%), Postives = 53/102 (51.96%), Query Frame = 1

Query: 10  YLMKLSLGMPPVSFSAIFYIGSDLVWTQCKP---CFEQPTSVYDPAQLSSFANVIANVTQ 69
           YLMKL +G PP    AI   GS++ WTQC P   C+EQ   ++DP++ S+F        +
Sbjct: 65  YLMKLQVGTPPFEIQAIIDTGSEITWTQCLPCVHCYEQNAPIFDPSKSSTFKE-----KR 124

Query: 70  IDVSSCPIVSMYVSVLLRLEYSYSYRDGSFSIGFLAFETLTL 109
            D  SCP             Y   Y D ++++G LA ET+TL
Sbjct: 125 CDGHSCP-------------YEVDYFDHTYTMGTLATETITL 148

BLAST of Cucsa.172950 vs. NCBI nr
Match: gi|225438315|ref|XP_002272802.1| (PREDICTED: aspartic proteinase nepenthesin-1 [Vitis vinifera])

HSP 1 Score: 161.8 bits (408), Expect = 1.5e-36
Identity = 110/309 (35.60%), Postives = 146/309 (47.25%), Query Frame = 1

Query: 8   GSYLMKLSLGMPPVSFSAIFYIGSDLVWTQCKPC---FEQPTSVYDPAQLSSFANVIAN- 67
           G +LMKL++G P  ++SAI   GSDL+WTQCKPC   F+QPT ++DP + SSF+ +  + 
Sbjct: 95  GEFLMKLAIGTPAETYSAIMDTGSDLIWTQCKPCKDCFDQPTPIFDPKKSSSFSKLPCSS 154

Query: 68  --VTQIDVSSCPIVSMYVSVLLRLEYSYSYRDGSFSIGFLAFETLTLGEANQQ------- 127
                + +SSC             EY YSY D S + G LA ET   G+A+         
Sbjct: 155 DLCAALPISSCSD---------GCEYLYSYGDYSSTQGVLATETFAFGDASVSKIGFGCG 214

Query: 128 ------VYTQDIGLT--------------------------QGAGIDRFNIGP------- 187
                  ++Q  GL                              GI    +G        
Sbjct: 215 EDNDGSGFSQGAGLVGLGRGPLSLISQLGEPKFSYCLTSMDDSKGISSLLVGSEATMKNA 274

Query: 188 -TTPLIQNPFDPSYYYLSLQAVP---------SSWFKLNNDGTSGVNIDSSTSIMYFRGC 238
            TTPLIQNP  PS+YYLSL+ +           S F + NDG+ G+ IDS T+I Y    
Sbjct: 275 ITTPLIQNPSQPSFYYLSLEGISVGDTLLPIEKSTFSIQNDGSGGLIIDSGTTITYLEDS 334

BLAST of Cucsa.172950 vs. NCBI nr
Match: gi|225437854|ref|XP_002264056.1| (PREDICTED: aspartic proteinase nepenthesin-1 [Vitis vinifera])

HSP 1 Score: 153.7 bits (387), Expect = 4.1e-34
Identity = 106/309 (34.30%), Postives = 148/309 (47.90%), Query Frame = 1

Query: 8   GSYLMKLSLGMPPVSFSAIFYIGSDLVWTQCKPC---FEQPTSVYDPAQLSSFANVIAN- 67
           G +LM L++G P  ++SAI   GSDL+WTQCKPC   F+QPT ++DP + SSF+ +  + 
Sbjct: 95  GEFLMNLAIGTPAETYSAIMDTGSDLIWTQCKPCKVCFDQPTPIFDPEKSSSFSKLPCSS 154

Query: 68  --VTQIDVSSCPIVSMYVSVLLRLEYSYSYRDGSFSIGFLAFETLTLGEANQ-------- 127
                + +SSC             EY YSY D S + G LA ET T G+A+         
Sbjct: 155 DLCVALPISSCSD---------GCEYRYSYGDHSSTQGVLATETFTFGDASVSKIGFGCG 214

Query: 128 -----QVYTQDIGLT----------QGAGIDRFNIGPT---------------------- 187
                + Y+Q  GL              G+ +F+   T                      
Sbjct: 215 EDNRGRAYSQGAGLVGLGRGPLSLISQLGVPKFSYCLTSIDDSKGISTLLVGSEATVKSA 274

Query: 188 --TPLIQNPFDPSYYYLSLQAVP---------SSWFKLNNDGTSGVNIDSSTSIMYFRGC 238
             TPLIQNP  PS+YYLSL+ +           S F + +DG+ G+ IDS T+I Y +  
Sbjct: 275 IPTPLIQNPSRPSFYYLSLEGISVGDTLLPIEKSTFSIQDDGSGGLIIDSGTTITYLKDS 334

BLAST of Cucsa.172950 vs. NCBI nr
Match: gi|147862576|emb|CAN79341.1| (hypothetical protein VITISV_006338 [Vitis vinifera])

HSP 1 Score: 151.4 bits (381), Expect = 2.0e-33
Identity = 105/309 (33.98%), Postives = 148/309 (47.90%), Query Frame = 1

Query: 8   GSYLMKLSLGMPPVSFSAIFYIGSDLVWTQCKPC---FEQPTSVYDPAQLSSFANVIAN- 67
           G +LM L++G P  ++SAI   GSDL+WTQCKPC   F+QPT ++DP + SSF+ +  + 
Sbjct: 95  GEFLMNLAIGTPAETYSAIMDTGSDLIWTQCKPCKVCFDQPTPIFDPEKSSSFSKLPCSS 154

Query: 68  --VTQIDVSSCPIVSMYVSVLLRLEYSYSYRDGSFSIGFLAFETLTLGEANQ-------- 127
                + +SSC             EY YSY D S + G LA ET T G+A+         
Sbjct: 155 DLCVALPISSCSD---------GCEYRYSYGDHSSTQGVLATETFTFGDASVSKIGFGCG 214

Query: 128 -----QVYTQDIGLT----------QGAGIDRFNIGPT---------------------- 187
                + Y+Q  GL              G+ +F+   T                      
Sbjct: 215 EDNRGRAYSQGAGLVGLGRGPLSLISQLGVPKFSYCLTSIDDSKGISTLLVGSEATVKSA 274

Query: 188 --TPLIQNPFDPSYYYLSLQAVP---------SSWFKLNNDGTSGVNIDSSTSIMYFRGC 238
             TPLIQNP  PS+YYLSL+ +           S F + +DG+ G+ IDS T+I Y +  
Sbjct: 275 IPTPLIQNPSRPSFYYLSLEGISVGDTLLPIEKSTFSIQDDGSGGLIIDSGTTITYLKDN 334

BLAST of Cucsa.172950 vs. NCBI nr
Match: gi|413918484|gb|AFW58416.1| (hypothetical protein ZEAMMB73_998053 [Zea mays])

HSP 1 Score: 147.5 bits (371), Expect = 2.9e-32
Identity = 113/320 (35.31%), Postives = 149/320 (46.56%), Query Frame = 1

Query: 8   GSYLMKLSLGMPPVSFSAIFYIGSDLVWTQCKPC---FEQPTSVYDPAQLSSFANV---I 67
           G +LM LS+G P + ++AI   GSDLVWTQCKPC   F Q T V+DPA  S++A +    
Sbjct: 114 GEFLMDLSVGTPALPYAAIVDTGSDLVWTQCKPCVECFNQTTPVFDPAASSTYAALPCSS 173

Query: 68  ANVTQIDVSSCPIVSMYVSVLLRLEYSYSYRDGSFSIGFLAFETLTLGEANQQV------ 127
           A    +  S+C   S   S      Y+Y+Y D S + G LA ET TL  A Q+V      
Sbjct: 174 ALCADLPTSTCASSSSSSSASSPCGYTYTYGDASSTQGVLATETFTL--ARQKVPGVAFG 233

Query: 128 ---YTQDIGLTQGA----------------GIDRFNI----------------------- 187
                +  G TQGA                GIDRF+                        
Sbjct: 234 CGDTNEGDGFTQGAGLVGLGRGPLSLVSQLGIDRFSYCLTSLDDAAGRSPLLLGSAAGIS 293

Query: 188 -------GPTTPLIQNPFDPSYYYLSLQ---------AVPSSWFKLNNDGTSGVNIDSST 237
                    TTPL++NP  PS+YY+SL          A+PSS F + +DGT GV +DS T
Sbjct: 294 ASAATAPAQTTPLVKNPSQPSFYYVSLTGLTVGSTRLALPSSAFAIQDDGTGGVIVDSGT 353

BLAST of Cucsa.172950 vs. NCBI nr
Match: gi|670447941|ref|XP_008664185.1| (PREDICTED: aspartic proteinase nepenthesin-1 [Zea mays])

HSP 1 Score: 147.5 bits (371), Expect = 2.9e-32
Identity = 113/320 (35.31%), Postives = 149/320 (46.56%), Query Frame = 1

Query: 8   GSYLMKLSLGMPPVSFSAIFYIGSDLVWTQCKPC---FEQPTSVYDPAQLSSFANV---I 67
           G +LM LS+G P + ++AI   GSDLVWTQCKPC   F Q T V+DPA  S++A +    
Sbjct: 153 GEFLMDLSVGTPALPYAAIVDTGSDLVWTQCKPCVECFNQTTPVFDPAASSTYAALPCSS 212

Query: 68  ANVTQIDVSSCPIVSMYVSVLLRLEYSYSYRDGSFSIGFLAFETLTLGEANQQV------ 127
           A    +  S+C   S   S      Y+Y+Y D S + G LA ET TL  A Q+V      
Sbjct: 213 ALCADLPTSTCASSSSSSSASSPCGYTYTYGDASSTQGVLATETFTL--ARQKVPGVAFG 272

Query: 128 ---YTQDIGLTQGA----------------GIDRFNI----------------------- 187
                +  G TQGA                GIDRF+                        
Sbjct: 273 CGDTNEGDGFTQGAGLVGLGRGPLSLVSQLGIDRFSYCLTSLDDAAGRSPLLLGSAAGIS 332

Query: 188 -------GPTTPLIQNPFDPSYYYLSLQ---------AVPSSWFKLNNDGTSGVNIDSST 237
                    TTPL++NP  PS+YY+SL          A+PSS F + +DGT GV +DS T
Sbjct: 333 ASAATAPAQTTPLVKNPSQPSFYYVSLTGLTVGSTRLALPSSAFAIQDDGTGGVIVDSGT 392

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
NEP1_NEPGR1.2e-1437.68Aspartic proteinase nepenthesin-1 OS=Nepenthes gracilis GN=nep1 PE=1 SV=1[more]
NEP2_NEPGR2.8e-1436.67Aspartic proteinase nepenthesin-2 OS=Nepenthes gracilis GN=nep2 PE=1 SV=1[more]
ASPA_ARATH7.5e-1232.24Aspartyl protease family protein At5g10770 OS=Arabidopsis thaliana GN=At5g10770 ... [more]
CDR1_ARATH2.9e-1135.34Aspartic proteinase CDR1 OS=Arabidopsis thaliana GN=CDR1 PE=1 SV=1[more]
AED1_ARATH2.7e-0930.72Aspartyl protease AED1 OS=Arabidopsis thaliana GN=AED1 PE=2 SV=1[more]
Match NameE-valueIdentityDescription
M0U7H4_MUSAM1.2e-3736.36Uncharacterized protein OS=Musa acuminata subsp. malaccensis PE=3 SV=1[more]
F6H4G8_VITVI1.1e-3635.60Putative uncharacterized protein OS=Vitis vinifera GN=VIT_07s0031g02900 PE=3 SV=... [more]
A0A0D9W5K9_9ORYZ9.9e-3535.95Uncharacterized protein OS=Leersia perrieri PE=3 SV=1[more]
F6HZI3_VITVI2.9e-3434.30Putative uncharacterized protein OS=Vitis vinifera GN=VIT_07s0005g03140 PE=3 SV=... [more]
A5BUH7_VITVI1.4e-3333.98Putative uncharacterized protein OS=Vitis vinifera GN=VITISV_006338 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
AT2G03200.14.4e-1839.57 Eukaryotic aspartyl protease family protein[more]
AT1G64830.16.6e-1433.05 Eukaryotic aspartyl protease family protein[more]
AT5G10770.14.3e-1332.24 Eukaryotic aspartyl protease family protein[more]
AT5G33340.11.6e-1235.34 Eukaryotic aspartyl protease family protein[more]
AT2G28010.11.4e-1137.25 Eukaryotic aspartyl protease family protein[more]
Match NameE-valueIdentityDescription
gi|225438315|ref|XP_002272802.1|1.5e-3635.60PREDICTED: aspartic proteinase nepenthesin-1 [Vitis vinifera][more]
gi|225437854|ref|XP_002264056.1|4.1e-3434.30PREDICTED: aspartic proteinase nepenthesin-1 [Vitis vinifera][more]
gi|147862576|emb|CAN79341.1|2.0e-3333.98hypothetical protein VITISV_006338 [Vitis vinifera][more]
gi|413918484|gb|AFW58416.1|2.9e-3235.31hypothetical protein ZEAMMB73_998053 [Zea mays][more]
gi|670447941|ref|XP_008664185.1|2.9e-3235.31PREDICTED: aspartic proteinase nepenthesin-1 [Zea mays][more]
The following terms have been associated with this gene:
Vocabulary: INTERPRO
TermDefinition
IPR001461Aspartic_peptidase_A1
IPR021109Peptidase_aspartic_dom_sf
Vocabulary: Molecular Function
TermDefinition
GO:0004190aspartic-type endopeptidase activity
Vocabulary: Biological Process
TermDefinition
GO:0006508proteolysis
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0043170 macromolecule metabolic process
biological_process GO:0006508 proteolysis
cellular_component GO:0005575 cellular_component
molecular_function GO:0004190 aspartic-type endopeptidase activity

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Cucsa.172950.1Cucsa.172950.1mRNA


Analysis Name: InterPro Annotations of cucumber (Gy14)
Date Performed: 2017-01-17
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001461Aspartic peptidase A1 familyPANTHERPTHR13683ASPARTYL PROTEASEScoord: 83..232
score: 3.9E-44coord: 8..58
score: 3.9
IPR021109Aspartic peptidase domainGENE3DG3DSA:2.40.70.10coord: 192..233
score: 4.7E-5coord: 5..122
score: 8.8
IPR021109Aspartic peptidase domainunknownSSF50630Acid proteasescoord: 6..233
score: 1.75
NoneNo IPR availablePANTHERPTHR13683:SF324SUBFAMILY NOT NAMEDcoord: 83..232
score: 3.9E-44coord: 8..58
score: 3.9

The following gene(s) are orthologous to this gene:
GeneOrthologueOrganismBlock
Cucsa.172950CSPI06G20500Wild cucumber (PI 183967)cgycpiB271
The following gene(s) are paralogous to this gene:

None

The following block(s) are covering this gene:
GeneOrganismBlock
Cucsa.172950Cucumber (Chinese Long) v3cgycucB276
Cucsa.172950Cucumber (Chinese Long) v2cgycuB263