Cucsa.172480 (gene) Cucumber (Gy14) v1

BLAST of Cucsa.172480 vs. Swiss-Prot
Match: P2C04_ARATH (Probable protein phosphatase 2C 4 OS=Arabidopsis thaliana GN=PLL5 PE=2 SV=1)

HSP 1 Score: 880.6 bits (2274), Expect = 1.2e-254
Identity = 483/741 (65.18%), Postives = 558/741 (75.30%), Query Frame = 1

Query: 1   MGNGIGKLGVCFTGINDGRRRP-DISVLLTNPLDEGLGHSFCYVRPDPLRLSLSKVHSEE 60
           MGNG+ KL +CFTG    R RP DISVLL +PLDEGLGHSFCYVRPDP  +S SKVHSEE
Sbjct: 1   MGNGVTKLSICFTGGGGERLRPKDISVLLPDPLDEGLGHSFCYVRPDPTLISSSKVHSEE 60

Query: 61  ---TTTFRSISGASVSANTSTPLSTALIDLYSYNSIDRAAAFESSTSFASIPLQPIPRNM 120
              TTTFR+ISGASVSANT+TPLST+L D Y +  IDRAAAFES+TSF+SIPLQPIP++ 
Sbjct: 61  DTTTTTFRTISGASVSANTATPLSTSLYDPYGH--IDRAAAFESTTSFSSIPLQPIPKS- 120

Query: 121 INSGPISGNFSGIPCSGPLERGFLSGPIERGFMSGPLDR-GIFSGPLD--NNSTSNEFQR 180
             SGPI      +  SGP+ERGFLSGPIERGFMSGPLDR G+FSGPLD  N+   ++FQR
Sbjct: 121 --SGPI------VLGSGPIERGFLSGPIERGFMSGPLDRVGLFSGPLDKPNSDHHHQFQR 180

Query: 181 SFSHGAGFNSRSRSRKSRLIRILRTAISKSLHRGQNSIVAPIKPVKEPD-WIVLPEK--- 240
           SFSHG     R  SRK  L+RILR AISK++ RGQNSIVAPIK VK+ D W +  EK   
Sbjct: 181 SFSHGLAL--RVGSRKRSLVRILRRAISKTMSRGQNSIVAPIKSVKDSDNWGIRSEKSRN 240

Query: 241 -HNENLTVSSLNFSSEGSLDDDDSMESQNLQWAQGKAGEDRVHVVVSEEHGWVFVGIYDG 300
            HNENLTV+SLNFSSE SLDDD S+E+QNLQWAQGKAGEDRVHVVVSEEHGW+FVGIYDG
Sbjct: 241 LHNENLTVNSLNFSSEVSLDDDVSLENQNLQWAQGKAGEDRVHVVVSEEHGWLFVGIYDG 300

Query: 301 FNGPDAPDYLLSNLYSSVHKELKGLLWDEKLDSAIVAAPATSPVQSENPLSETRDLGRNR 360
           FNGPDAPDYLLS+LY  VH+ELKGLLWD+                  N  S+++DL R+ 
Sbjct: 301 FNGPDAPDYLLSHLYPVVHRELKGLLWDD-----------------SNVESKSQDLERSN 360

Query: 361 VSDACSQCVEQENHPCVSGDGNSGLNSRKRKGKNLRGKFRGAAEKW---------EDNQR 420
             ++CS   ++++  C                           E+W         +D  R
Sbjct: 361 GDESCSN--QEKDETC---------------------------ERWWRCEWDRESQDLDR 420

Query: 421 RWKCEWDR----ERLELDRRLKEQLNRSGSNGSRLINHSDVLKALSQALKKTEEAYLDIA 480
           R K +  R    +RL     + E L+++                     +KTEEAYLD A
Sbjct: 421 RLKEQISRRSGSDRLTNHSEVLEALSQAL--------------------RKTEEAYLDTA 480

Query: 481 DKMLDENPELALMGSCVLVMLMKGDDVYVMNVGDSRAVLAQKAEPDYWLGKIRQDLERIN 540
           DKMLDENPELALMGSCVLVMLMKG+D+YVMNVGDSRAVL QK+EPDYWL KIRQDLERIN
Sbjct: 481 DKMLDENPELALMGSCVLVMLMKGEDIYVMNVGDSRAVLGQKSEPDYWLAKIRQDLERIN 540

Query: 541 EEP-VHDIEALDGDGAFTSANLTAFQLSMDHSTSVDEEVQRIKNEHPDDAGAVMNDRVKG 600
           EE  ++D+E  +GD +    NL+AFQL++DHST+++EEV+RI+NEHPDD  AV N+RVKG
Sbjct: 541 EETMMNDLEGCEGDQSSLVPNLSAFQLTVDHSTNIEEEVERIRNEHPDDVTAVTNERVKG 600

Query: 601 SLKVTRAFGAGFLKQPKWNNALLEMFRIDYVGRSPYINCLPSLYHHRLGPKDRFLILSSD 660
           SLKVTRAFGAGFLKQPKWNNALLEMF+IDYVG+SPYINCLPSLYHHRLG KDRFLILSSD
Sbjct: 601 SLKVTRAFGAGFLKQPKWNNALLEMFQIDYVGKSPYINCLPSLYHHRLGSKDRFLILSSD 660

Query: 661 GLYQYFTNEEAVSEVELFIALQPEGDPAQHLVELLLFRAAKRAGMDFHELLEIPQGDRRK 716
           GLYQYFTNEEAVSEVELFI LQPEGDPAQHLV+ LLFRAAK+AGMDFHELLEIPQG+RR+
Sbjct: 661 GLYQYFTNEEAVSEVELFITLQPEGDPAQHLVQELLFRAAKKAGMDFHELLEIPQGERRR 662

BLAST of Cucsa.172480 vs. Swiss-Prot
Match: P2C23_ARATH (Probable protein phosphatase 2C 23 OS=Arabidopsis thaliana GN=PLL4 PE=2 SV=1)

HSP 1 Score: 825.5 bits (2131), Expect = 4.6e-238
Identity = 452/735 (61.50%), Postives = 526/735 (71.56%), Query Frame = 1

Query: 1   MGNGIGKLGVCFTGINDGRRRPDISVLLTNPLDEGLGHSFCYVRPDPLRLSLSKVHSEE- 60
           MGNGIGKL  C TG     ++P++S+L  +PLDEGLGHSFCYVRPDP R+S SKVHSEE 
Sbjct: 1   MGNGIGKLSKCLTGGAGRNKKPELSILEPDPLDEGLGHSFCYVRPDPTRVSSSKVHSEEE 60

Query: 61  TTTFRSISGASVSANTSTPLSTALIDLYSYNSIDRAAAFESSTSFASIPLQPIPRNMINS 120
           TTTFR+ISGASVSANT+TPLST+L D Y +  IDRAAAFES+TSF+SIPLQPIPR+   S
Sbjct: 61  TTTFRTISGASVSANTATPLSTSLYDPYGH--IDRAAAFESTTSFSSIPLQPIPRS---S 120

Query: 121 GPISGNFSGIPCSGPLERGFLSGPIERGFMSGPLDRGIFSGPLDNNSTSNEFQRSFSHGA 180
           GPI      +P SGPLERGFLSGPIERGFMSGPLD    SGP+D  + S++FQRSFSHG 
Sbjct: 121 GPI------VPGSGPLERGFLSGPIERGFMSGPLDGS--SGPIDGKTGSDQFQRSFSHGL 180

Query: 181 GFNSRSRSRKSRLIRILRTAISKSLHRGQNSIVAPIKPVKEPDWIVLPEKHN-----ENL 240
             N R  SRK  L+R+LR AISK++ RGQNSIVAPIKPVKEPDW+   +K        NL
Sbjct: 181 A-NLRVGSRKGSLVRVLRRAISKTITRGQNSIVAPIKPVKEPDWVFGSDKTRIHQIENNL 240

Query: 241 TVSSLNFSSEGSL-DDDDSMESQNLQWAQGKAGEDRVHVVVSEEHGWVFVGIYDGFNGPD 300
           TV+SLNFSSEGSL DDD S+ESQNLQWAQGKAGEDRVHVVVSEEHGW+FVGIYDGFNGPD
Sbjct: 241 TVNSLNFSSEGSLLDDDVSLESQNLQWAQGKAGEDRVHVVVSEEHGWLFVGIYDGFNGPD 300

Query: 301 APDYLLSNLYSSVHKELKGLLWDEKLDSAIVAAPATSPVQSENPLSETRDLGRNRVSDAC 360
           APDYLLS+LY +VH+ELKGLLWD+         P T    S+    E RD          
Sbjct: 301 APDYLLSHLYPAVHRELKGLLWDD---------PKTDAKSSDEADVENRD---------- 360

Query: 361 SQCVEQENHPCVSGDGNSGLNSRKRKGKNLRGKFRGAAEKWE--------DNQRRWKCEW 420
                               +S ++K KN     R    +W+        D       + 
Sbjct: 361 --------------------SSSEKKSKNWEESQRRWRCEWDRDLDRLLKDRSNGLDLDP 420

Query: 421 DRERLELDRRLKEQLNRSG----SNGSRLINHSDVLKALSQALKKTEEAYLDIADKMLDE 480
           D    ++ + L + L ++      N   +++ +  L  +                     
Sbjct: 421 DPNSSDVLKALSQALRKTEEAYLENADMMLDENPELALMGS------------------- 480

Query: 481 NPELALMGSCVLVMLMKGDDVYVMNVGDSRAVLAQKAEPDYWLGKIRQDLERINEEPVHD 540
                    CVLVMLMKG+DVY+MNVGDSRAVL QKAE DYW+GKI+QDLERINEE ++D
Sbjct: 481 ---------CVLVMLMKGEDVYLMNVGDSRAVLGQKAESDYWIGKIKQDLERINEETMND 540

Query: 541 IEAL-DGDGAFTSANLTAFQLSMDHSTSVDEEVQRIKNEHPDDAGAVMNDRVKGSLKVTR 600
            +   DG+GA     L+AFQL++DHST+V+EEV RI+ EHPDDA AV N+RVKGSLKVTR
Sbjct: 541 FDGCGDGEGASLVPTLSAFQLTVDHSTNVEEEVNRIRKEHPDDASAVSNERVKGSLKVTR 600

Query: 601 AFGAGFLKQPKWNNALLEMFRIDYVGRSPYINCLPSLYHHRLGPKDRFLILSSDGLYQYF 660
           AFGAGFLKQPKWNNALLEMF+IDY G SPYINCLPSLYHHRLG KD+FLILSSDGLYQYF
Sbjct: 601 AFGAGFLKQPKWNNALLEMFQIDYKGTSPYINCLPSLYHHRLGSKDQFLILSSDGLYQYF 654

Query: 661 TNEEAVSEVELFIALQPEGDPAQHLVELLLFRAAKRAGMDFHELLEIPQGDRRKYHDDVS 716
           TNEEAVSEVELFI LQPEGDPAQHLV+ LLFRAAK+AGMDFHELLEIPQG+RR+YHDDVS
Sbjct: 661 TNEEAVSEVELFITLQPEGDPAQHLVQELLFRAAKKAGMDFHELLEIPQGERRRYHDDVS 654

BLAST of Cucsa.172480 vs. Swiss-Prot
Match: P2C66_ARATH (Probable protein phosphatase 2C 66 OS=Arabidopsis thaliana GN=PLL2 PE=2 SV=1)

HSP 1 Score: 622.9 bits (1605), Expect = 4.5e-177
Identity = 371/733 (50.61%), Postives = 478/733 (65.21%), Query Frame = 1

Query: 1   MGNGIGKLGVCFTGINDGR--RRPDISVLLTNPLDEGLGHSFCYVRPDPLRLSLSKVHSE 60
           MGNG+  L  C TG   G   RR D+S++      +GLGHSFCY+RPD   + L    S 
Sbjct: 1   MGNGVTTLTGCCTGTLAGEISRRYDVSLV-----HDGLGHSFCYIRPDLPGVVLPSPESP 60

Query: 61  ------ETTTFRSISGASVSANTSTPLSTALIDLYS--YNSIDRAAAFESSTSFASIPLQ 120
                 + TTFRSISGASVSAN ST LS AL       Y+S   A+AFESS +FAS+PLQ
Sbjct: 61  LRSDHIQETTFRSISGASVSANPSTALSGALSSDSDCPYSSAVSASAFESSGNFASLPLQ 120

Query: 121 PIPRNMI-NSGPISGNFSGIPCSGPLERGFLSGPIERGFMSGPLDRGIFSGPLDNNSTSN 180
           P+PR     SGPI             E G  S P ER F+SGP++ G++SGP+++   + 
Sbjct: 121 PVPRGSTWQSGPIVN-----------ESGLGSAPFERRFLSGPIESGLYSGPIESTKKTE 180

Query: 181 EFQRSFSHGAGFNSRSRSRKSRLI--RILRTAISKSLH-RGQNSIVAPIKPVKEPDWIVL 240
           + +           + +S+K+ L    +    IS +   R + S++ PI      D   L
Sbjct: 181 KEKPK-----KIRKKPKSKKNFLTFKTLFANLISNNNKPRLKKSVIEPINGSDSSDSGRL 240

Query: 241 PEKHNENLTVSSLNFSSEGSLDDDDSMESQN----LQWAQGKAGEDRVHVVVSEEHGWVF 300
              H   +T S  N + +  L+++D  +S N    +QWAQGKAGEDRVHVVVSE++GWVF
Sbjct: 241 --HHEPVITSSRSNENPKSDLEEEDEKQSMNSVLDVQWAQGKAGEDRVHVVVSEDNGWVF 300

Query: 301 VGIYDGFNGPDAPDYLLSNLYSSVHKELKGLLW-DEKLDSAIVAAPATSPVQSENPLSET 360
           VGIYDGF+GPDAPDYLL+NLY++V KEL GLLW DEKL S             EN +++T
Sbjct: 301 VGIYDGFSGPDAPDYLLNNLYTAVQKELNGLLWNDEKLRSL-----------GENGMTKT 360

Query: 361 RDLGRNRVSDACSQCVEQENHPCVSGDGNSGLNSRKRKGKNLRGKFRGAAEKWEDNQRRW 420
                 + SD       +EN P ++ D ++  +  + + K+L+                W
Sbjct: 361 -----GKCSDEEDPESGKENCPVINND-DAVASGARNQAKSLK----------------W 420

Query: 421 KCEWDRERLELDRRLKEQLNRSGSNGSRLINHSDVLKALSQALKKTEEAYLDIADKMLDE 480
           +CEW+++     +    + ++ GSN S   NH DVLKAL QAL+KTE+AYL++AD+M+ E
Sbjct: 421 RCEWEKKSNNKTKS-DNRCDQKGSN-STTTNHKDVLKALLQALRKTEDAYLELADQMVKE 480

Query: 481 NPELALMGSCVLVMLMKGDDVYVMNVGDSRAVLAQKAEPDYWLGKIRQ-DLERINEEPVH 540
           NPELALMGSCVLV LMKG+DVYVMNVGDSRAVL +K  P+   G+ RQ +LERI E+   
Sbjct: 481 NPELALMGSCVLVTLMKGEDVYVMNVGDSRAVLGRK--PNLATGRKRQKELERIREDSSL 540

Query: 541 DIEALDGDGAFTSANLTAFQLSMDHSTSVDEEVQRIKNEHPDDAGAVMNDRVKGSLKVTR 600
           + + +  +GA  +  L   QL+M+HST ++EEV+RIK EHPDD  AV NDRVKG LKVTR
Sbjct: 541 EDKEILMNGAMRNT-LVPLQLNMEHSTRIEEEVRRIKKEHPDDDCAVENDRVKGYLKVTR 600

Query: 601 AFGAGFLKQPKWNNALLEMFRIDYVGRSPYINCLPSLYHHRLGPKDRFLILSSDGLYQYF 660
           AFGAGFLKQPKWN+ALLEMFRIDY+G SPYI C PSL HH+L  +D+FLILSSDGLY+YF
Sbjct: 601 AFGAGFLKQPKWNDALLEMFRIDYIGTSPYITCSPSLCHHKLTSRDKFLILSSDGLYEYF 660

Query: 661 TNEEAVSEVELFIALQPEGDPAQHLVELLLFRAAKRAGMDFHELLEIPQGDRRKYHDDVS 714
           +N+EA+ EVE FI+  PEGDPAQHL++ +L RAA + GMDFHELLEIPQGDRR+YHDDVS
Sbjct: 661 SNQEAIFEVESFISAFPEGDPAQHLIQEVLLRAANKFGMDFHELLEIPQGDRRRYHDDVS 672

BLAST of Cucsa.172480 vs. Swiss-Prot
Match: P2C35_ORYSJ (Protein phosphatase 2C 35 OS=Oryza sativa subsp. japonica GN=XB15 PE=1 SV=1)

HSP 1 Score: 599.4 bits (1544), Expect = 5.3e-170
Identity = 363/740 (49.05%), Postives = 449/740 (60.68%), Query Frame = 1

Query: 1   MGNGIGKLGVCFTGINDGR--RRPDISVLLTNPL-DEGLGHSFCYVRPDPLRLSLSK--- 60
           MGN +     C  G   GR  R    + L ++P  DEGLGHSFCYVRPD   +  S    
Sbjct: 1   MGNSLACF--CCGGGAGGRGGRHVAPAALPSDPAYDEGLGHSFCYVRPDKFVVPFSADDL 60

Query: 61  --------VHSEETTTFRSISGASVSANTSTPLSTALIDLYSYNSIDRAAA---FESSTS 120
                       E TTFR+ISGA++SAN STPLST+++ L    S   A A   FESS S
Sbjct: 61  VADAKAAAAAEGEATTFRAISGAALSANVSTPLSTSVLLLMPEESSASATASSGFESSES 120

Query: 121 FASIPLQPIPRNMINSGPISGNFSGIPCSGPLERGFLSGPIERGFMSGPLDRGIFSGPLD 180
           FA++PLQP+PR   +SGPIS  FSG         GF+SGP+ERGF SGPLD  + SGPL 
Sbjct: 121 FAAVPLQPVPR--FSSGPISAPFSG---------GFMSGPLERGFQSGPLDAALLSGPLP 180

Query: 181 NNSTSNEFQRSFSHGAGFNSRSRSRKSRLIRILRTAISKSLHRGQNSIVAPIKPVKEPDW 240
             +TS                   R    +  LR ++S    R +N   A          
Sbjct: 181 GTATSG------------------RMGGAVPALRRSLSHGGRRLRNFTRA---------L 240

Query: 241 IVLPEKHNENLTVSSLNFSSEGSLDDDDSMESQNLQWAQGKAGEDRVHVVVSEEHGWVFV 300
           +   EK  ++  + S + ++          +   LQWAQGKAGEDRVHVVVSEE GWVFV
Sbjct: 241 LARTEKFQDSADLGSPDAAAAAVAACGG--DPCGLQWAQGKAGEDRVHVVVSEERGWVFV 300

Query: 301 GIYDGFNGPDAPDYLLSNLYSSVHKELKGLLWDEKLDSA--------IVAAPATSPVQSE 360
           GIYDGFNGPDA D+L+SNLY++VH+EL+GLLWD++  +           +AP+T+   ++
Sbjct: 301 GIYDGFNGPDATDFLVSNLYAAVHRELRGLLWDQREQNVQHDQRPDQPGSAPSTTASDNQ 360

Query: 361 NPLSETRDLGRNRVSDACSQCVEQENHPCVSGDGNSGLNSRKRKGKNLRGKFRGAAEKWE 420
           +     R   R+R                       G +  +R+ K    + R  +    
Sbjct: 361 DQWGRRRRTRRSRPP--------------------RGADDDQRRWKCEWEQERDCSNLKP 420

Query: 421 DNQRRWKCEWDRERLELDRRLKEQLNRSGSNGSRLINHSDVLKALSQALKKTEEAYLDIA 480
             Q+R +C  + + +                         VLKAL++AL +TEEAYLDIA
Sbjct: 421 PTQQRLRCNSENDHVA------------------------VLKALTRALHRTEEAYLDIA 480

Query: 481 DKMLDENPELALMGSCVLVMLMKGDDVYVMNVGDSRAVLAQKAEPDYWLGKIRQDLERIN 540
           DKM+ E PELALMGSCVL MLMKG+D+Y+MNVGDSRAVLA              DLE+I+
Sbjct: 481 DKMVGEFPELALMGSCVLAMLMKGEDMYIMNVGDSRAVLATMDS---------VDLEQIS 540

Query: 541 EEPVHDIEALDGDGAFTSANLTAFQLSMDHSTSVDEEVQRIKNEHPDDAGAVMNDRVKGS 600
           +       + DG        L+A QL+ DHSTSV+EEV RI+NEHPDD  A+  DRVKGS
Sbjct: 541 Q------GSFDGSVGDCPPCLSAVQLTSDHSTSVEEEVIRIRNEHPDDPSAISKDRVKGS 600

Query: 601 LKVTRAFGAGFLKQPKWNNALLEMFRIDYVGRSPYINCLPSLYHHRLGPKDRFLILSSDG 660
           LKVTRAFGAGFLKQPKWN+ALLEMFRIDYVG SPYI+C PSL+HH+L  +DRFLILSSDG
Sbjct: 601 LKVTRAFGAGFLKQPKWNDALLEMFRIDYVGSSPYISCNPSLFHHKLSTRDRFLILSSDG 639

Query: 661 LYQYFTNEEAVSEVELFIALQPEGDPAQHLVELLLFRAAKRAGMDFHELLEIPQGDRRKY 716
           LYQYFTNEEAV++VE+FIA  PEGDPAQHLVE +LFRAA +AGMDFHEL+EIP GDRR+Y
Sbjct: 661 LYQYFTNEEAVAQVEMFIATTPEGDPAQHLVEEVLFRAANKAGMDFHELIEIPHGDRRRY 639

BLAST of Cucsa.172480 vs. Swiss-Prot
Match: P2C36_ARATH (Probable protein phosphatase 2C 36 OS=Arabidopsis thaliana GN=PLL3 PE=2 SV=1)

HSP 1 Score: 573.9 bits (1478), Expect = 2.4e-162
Identity = 353/737 (47.90%), Postives = 452/737 (61.33%), Query Frame = 1

Query: 1   MGNGIGKLGVCFTGINDGRRRPDIS---VLLTNPLDEGLGHSFCYVRPDPLRLSLSKVHS 60
           MGNG+     C  G   G    +IS   V     + E LGHSFCYVRP  L  S S    
Sbjct: 1   MGNGVASFSGCCAGTTAG----EISGRYVTGVGLVQENLGHSFCYVRP-VLTGSKSSFPP 60

Query: 61  EE----------TTTFRSISGASVSANTSTPLSTALIDLYSYNSIDRAAAFESSTSFASI 120
           E           TTTFRSISGASVSANTST LST+L    S ++   A+AFESS  FAS+
Sbjct: 61  EPPLRPDPIPGTTTTFRSISGASVSANTSTALSTSL----STDTSGIASAFESSNRFASL 120

Query: 121 PLQPIPRNMINSGPISGNFSGIPCSGPLERGFLSGPIERGFMSGPLDRGIFSGPLDNNST 180
           PLQP+PR+     PI  +  G   SG  ER FLSGPIE G +SG                
Sbjct: 121 PLQPVPRS-----PIKKSDHG---SGLFERRFLSGPIESGLVSG--------------KK 180

Query: 181 SNEFQRSFSHGAGFNSRSRSRKSRLIRILRTAISKSLHRGQNSIVAPIKPVKEPDWI--- 240
           + E  +    G+   ++ + +KS         +  +L   + S++ PI      D     
Sbjct: 181 TKEKAKLKKSGSKSFTKPKLKKSESKIFTFKNVFTNLSCSKKSVIKPINGFDSFDGSSDT 240

Query: 241 --VLPEKHNENLTVSSLNFSSEGSLDD--DDSMESQNLQWAQGKAGEDRVHVVVSEEHGW 300
              +PE ++ +  VSS         +D  + ++E   +QWAQGKAGEDRVHV++SEE+GW
Sbjct: 241 DRYIPEINSLSTIVSSHEKPRIKEEEDKTESALEEPKIQWAQGKAGEDRVHVILSEENGW 300

Query: 301 VFVGIYDGFNGPDAPDYLLSNLYSSVHKELKGLLWDEKLDSAIVAAPATSPVQSENPLSE 360
           +FVGIYDGF+GPD PDYL+ NLY++V +ELKGLLW +K +S        S ++ ++ +  
Sbjct: 301 LFVGIYDGFSGPDPPDYLIKNLYTAVLRELKGLLWIDKGES--YNRNGESNIEKQSTVEH 360

Query: 361 TRDLGRNRVSDACSQCVEQENHPCVSGD----GNSGLNSRKRKGKNLRGKFRGAAEKWED 420
             D              +QEN P ++G+    G+  + S  +K                 
Sbjct: 361 ASDS-------------DQENCPVMNGNDVACGSRNITSDVKK----------------- 420

Query: 421 NQRRWKCEWDRERLELDRRLKEQLNRSGSNGSRLINHSDVLKALSQALKKTEEAYLDIAD 480
              +W+CEW+                + SN S  INH DVL+AL QAL+KTEE++    D
Sbjct: 421 --LQWRCEWEH---------------NSSNKSNNINHKDVLRALQQALEKTEESF----D 480

Query: 481 KMLDENPELALMGSCVLVMLMKGDDVYVMNVGDSRAVLAQKAEPDYWLGKIRQDLERINE 540
            M++ENPELALMGSCVLV LMKG+DVYVM+VGDSRAVLA++  P+    K++++LER+ E
Sbjct: 481 LMVNENPELALMGSCVLVTLMKGEDVYVMSVGDSRAVLARR--PNVEKMKMQKELERVKE 540

Query: 541 EPVHDIEALDGDGAFTSANLTAFQLSMDHSTSVDEEVQRIKNEHPDDAGAVMNDRVKGSL 600
           E   +   +   G    + L   QL+ +HSTSV+EEV+RIK EHPDD  A+ N+RVKG L
Sbjct: 541 ESPLETLFITERGL---SLLVPVQLNKEHSTSVEEEVRRIKKEHPDDILAIENNRVKGYL 600

Query: 601 KVTRAFGAGFLKQPKWNNALLEMFRIDYVGRSPYINCLPSLYHHRLGPKDRFLILSSDGL 660
           KVTRAFGAGFLKQPKWN ALLEMFRIDYVG SPYI C PSL+HHRL  +D+FLILSSDGL
Sbjct: 601 KVTRAFGAGFLKQPKWNEALLEMFRIDYVGTSPYITCSPSLHHHRLSSRDKFLILSSDGL 648

Query: 661 YQYFTNEEAVSEVELFIALQPEGDPAQHLVELLLFRAAKRAGMDFHELLEIPQGDRRKYH 714
           Y+YF+NEEA+ EV+ FI+  PEGDPAQHL++ +L RAAK+ GMDFHELLEIPQGDRR+YH
Sbjct: 661 YEYFSNEEAIFEVDSFISAFPEGDPAQHLIQEVLLRAAKKYGMDFHELLEIPQGDRRRYH 648

BLAST of Cucsa.172480 vs. TrEMBL
Match: A0A0A0KE92_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_6G190450 PE=4 SV=1)

HSP 1 Score: 1441.8 bits (3731), Expect = 0.0e+00
Identity = 715/715 (100.00%), Postives = 715/715 (100.00%), Query Frame = 1

Query: 1   MGNGIGKLGVCFTGINDGRRRPDISVLLTNPLDEGLGHSFCYVRPDPLRLSLSKVHSEET 60
           MGNGIGKLGVCFTGINDGRRRPDISVLLTNPLDEGLGHSFCYVRPDPLRLSLSKVHSEET
Sbjct: 1   MGNGIGKLGVCFTGINDGRRRPDISVLLTNPLDEGLGHSFCYVRPDPLRLSLSKVHSEET 60

Query: 61  TTFRSISGASVSANTSTPLSTALIDLYSYNSIDRAAAFESSTSFASIPLQPIPRNMINSG 120
           TTFRSISGASVSANTSTPLSTALIDLYSYNSIDRAAAFESSTSFASIPLQPIPRNMINSG
Sbjct: 61  TTFRSISGASVSANTSTPLSTALIDLYSYNSIDRAAAFESSTSFASIPLQPIPRNMINSG 120

Query: 121 PISGNFSGIPCSGPLERGFLSGPIERGFMSGPLDRGIFSGPLDNNSTSNEFQRSFSHGAG 180
           PISGNFSGIPCSGPLERGFLSGPIERGFMSGPLDRGIFSGPLDNNSTSNEFQRSFSHGAG
Sbjct: 121 PISGNFSGIPCSGPLERGFLSGPIERGFMSGPLDRGIFSGPLDNNSTSNEFQRSFSHGAG 180

Query: 181 FNSRSRSRKSRLIRILRTAISKSLHRGQNSIVAPIKPVKEPDWIVLPEKHNENLTVSSLN 240
           FNSRSRSRKSRLIRILRTAISKSLHRGQNSIVAPIKPVKEPDWIVLPEKHNENLTVSSLN
Sbjct: 181 FNSRSRSRKSRLIRILRTAISKSLHRGQNSIVAPIKPVKEPDWIVLPEKHNENLTVSSLN 240

Query: 241 FSSEGSLDDDDSMESQNLQWAQGKAGEDRVHVVVSEEHGWVFVGIYDGFNGPDAPDYLLS 300
           FSSEGSLDDDDSMESQNLQWAQGKAGEDRVHVVVSEEHGWVFVGIYDGFNGPDAPDYLLS
Sbjct: 241 FSSEGSLDDDDSMESQNLQWAQGKAGEDRVHVVVSEEHGWVFVGIYDGFNGPDAPDYLLS 300

Query: 301 NLYSSVHKELKGLLWDEKLDSAIVAAPATSPVQSENPLSETRDLGRNRVSDACSQCVEQE 360
           NLYSSVHKELKGLLWDEKLDSAIVAAPATSPVQSENPLSETRDLGRNRVSDACSQCVEQE
Sbjct: 301 NLYSSVHKELKGLLWDEKLDSAIVAAPATSPVQSENPLSETRDLGRNRVSDACSQCVEQE 360

Query: 361 NHPCVSGDGNSGLNSRKRKGKNLRGKFRGAAEKWEDNQRRWKCEWDRERLELDRRLKEQL 420
           NHPCVSGDGNSGLNSRKRKGKNLRGKFRGAAEKWEDNQRRWKCEWDRERLELDRRLKEQL
Sbjct: 361 NHPCVSGDGNSGLNSRKRKGKNLRGKFRGAAEKWEDNQRRWKCEWDRERLELDRRLKEQL 420

Query: 421 NRSGSNGSRLINHSDVLKALSQALKKTEEAYLDIADKMLDENPELALMGSCVLVMLMKGD 480
           NRSGSNGSRLINHSDVLKALSQALKKTEEAYLDIADKMLDENPELALMGSCVLVMLMKGD
Sbjct: 421 NRSGSNGSRLINHSDVLKALSQALKKTEEAYLDIADKMLDENPELALMGSCVLVMLMKGD 480

Query: 481 DVYVMNVGDSRAVLAQKAEPDYWLGKIRQDLERINEEPVHDIEALDGDGAFTSANLTAFQ 540
           DVYVMNVGDSRAVLAQKAEPDYWLGKIRQDLERINEEPVHDIEALDGDGAFTSANLTAFQ
Sbjct: 481 DVYVMNVGDSRAVLAQKAEPDYWLGKIRQDLERINEEPVHDIEALDGDGAFTSANLTAFQ 540

Query: 541 LSMDHSTSVDEEVQRIKNEHPDDAGAVMNDRVKGSLKVTRAFGAGFLKQPKWNNALLEMF 600
           LSMDHSTSVDEEVQRIKNEHPDDAGAVMNDRVKGSLKVTRAFGAGFLKQPKWNNALLEMF
Sbjct: 541 LSMDHSTSVDEEVQRIKNEHPDDAGAVMNDRVKGSLKVTRAFGAGFLKQPKWNNALLEMF 600

Query: 601 RIDYVGRSPYINCLPSLYHHRLGPKDRFLILSSDGLYQYFTNEEAVSEVELFIALQPEGD 660
           RIDYVGRSPYINCLPSLYHHRLGPKDRFLILSSDGLYQYFTNEEAVSEVELFIALQPEGD
Sbjct: 601 RIDYVGRSPYINCLPSLYHHRLGPKDRFLILSSDGLYQYFTNEEAVSEVELFIALQPEGD 660

Query: 661 PAQHLVELLLFRAAKRAGMDFHELLEIPQGDRRKYHDDVSIIVISLEGRIWRSCV 716
           PAQHLVELLLFRAAKRAGMDFHELLEIPQGDRRKYHDDVSIIVISLEGRIWRSCV
Sbjct: 661 PAQHLVELLLFRAAKRAGMDFHELLEIPQGDRRKYHDDVSIIVISLEGRIWRSCV 715

BLAST of Cucsa.172480 vs. TrEMBL
Match: F6GV16_VITVI (Putative uncharacterized protein OS=Vitis vinifera GN=VIT_06s0004g04600 PE=4 SV=1)

HSP 1 Score: 1152.5 bits (2980), Expect = 0.0e+00
Identity = 577/726 (79.48%), Postives = 639/726 (88.02%), Query Frame = 1

Query: 1   MGNGIGKLGVCFTGINDGRRRPDISVLLTNPLDEGLGHSFCYVRPDPLRLSLSKVHSEET 60
           MGNG  KL +CFTG    RRR DISVL+++PLDEGLGHSFCY+RPD  RLS SKVHSEET
Sbjct: 1   MGNGFAKLSICFTGEGGARRRQDISVLISDPLDEGLGHSFCYIRPDQSRLSSSKVHSEET 60

Query: 61  TTFRSISGASVSANTSTPLSTALIDLYSYNSIDRAAAFESSTSFASIPLQPIPRNMINSG 120
           TTFRSISGASVSANTSTPLSTA +DLYSYNSIDRA+AFESSTSF SIPLQPIPRN +NSG
Sbjct: 61  TTFRSISGASVSANTSTPLSTAFVDLYSYNSIDRASAFESSTSFTSIPLQPIPRNWMNSG 120

Query: 121 PISGNFSGIPCSGPLERGFLSGPIERGFMSGPLDRGIFSGPLDNNSTSNEFQRSFSHGAG 180
           PI G++ GIP SGPLERGFLSGPIERGFMSGP+DRG+FSGPL+ +ST ++FQRS+SHG G
Sbjct: 121 PIPGSYGGIPGSGPLERGFLSGPIERGFMSGPIDRGLFSGPLEKSST-DQFQRSYSHG-G 180

Query: 181 FNSRSRSRKSRLIRILRTAISKSLHRGQNSIVAPIK---PVKEPDWIVLPEKHNENLTVS 240
           F  R RSRK  LIR+L+ AISK++ RGQNSIVAPIK    VKEPDW+V  EKHNENLTVS
Sbjct: 181 FAFRPRSRKGSLIRVLQRAISKTISRGQNSIVAPIKGVVSVKEPDWLVGSEKHNENLTVS 240

Query: 241 SLNFSSEGSLDDDDSMESQNLQWAQGKAGEDRVHVVVSEEHGWVFVGIYDGFNGPDAPDY 300
           S+N SS+GSL+DDDS+ESQNLQWAQGKAGEDRVHVVVSEEHGWVFVGIYDGFNGPDAPDY
Sbjct: 241 SVNLSSDGSLEDDDSLESQNLQWAQGKAGEDRVHVVVSEEHGWVFVGIYDGFNGPDAPDY 300

Query: 301 LLSNLYSSVHKELKGLLWDEKLDSAIVAAPATSPVQSENPLSETRD--------LGRNRV 360
           LLSNLYS+VHKELKGLLWD+K +S  VAAPA+SPV SE   SE  D        L RNR+
Sbjct: 301 LLSNLYSAVHKELKGLLWDDKHESNPVAAPASSPVPSEASNSELEDSHLGSDVDLARNRM 360

Query: 361 SDACSQCVEQENHPCVSGDGNSGLNSRKRKGKNLRGKFRGAAEKWEDNQRRWKCEWDRER 420
            D CS C  QE +P  SGD     NS+++KGKN + K++GAA+KWE+NQRRWKCEWDRER
Sbjct: 361 VDGCSHCSYQEYYPSGSGDVKFDSNSKRKKGKNSKNKYKGAAKKWEENQRRWKCEWDRER 420

Query: 421 LELDRRLKEQLNRSGSNGSRLINHSDVLKALSQALKKTEEAYLDIADKMLDENPELALMG 480
           LELDRRLKEQLN S ++GSR INHSDVLKALSQAL+KTEE+YL+IADKM+ ENPELALMG
Sbjct: 421 LELDRRLKEQLNGSNTDGSRSINHSDVLKALSQALRKTEESYLEIADKMVMENPELALMG 480

Query: 481 SCVLVMLMKGDDVYVMNVGDSRAVLAQKAEPDYWLGKIRQDLERINEEPVHDIEALDGDG 540
           SCVLVMLMKG+DVYVMNVGDSRAVLAQKAE D WLGKIRQDLERINEE +HD+EA+D D 
Sbjct: 481 SCVLVMLMKGEDVYVMNVGDSRAVLAQKAEADVWLGKIRQDLERINEETLHDLEAMDNDN 540

Query: 541 AFTSANLTAFQLSMDHSTSVDEEVQRIKNEHPDDAGAVMNDRVKGSLKVTRAFGAGFLKQ 600
           +     L+AFQL++DHSTSV+EEV+RIKNEHPDDA AVMNDRVKGSLKVTRAFGAGFLKQ
Sbjct: 541 SNMIPTLSAFQLTVDHSTSVEEEVRRIKNEHPDDACAVMNDRVKGSLKVTRAFGAGFLKQ 600

Query: 601 PKWNNALLEMFRIDYVGRSPYINCLPSLYHHRLGPKDRFLILSSDGLYQYFTNEEAVSEV 660
           PKWNNALLEMFRIDYVG SPYI+CLPSLYHHRLGP+DRFLILSSDGLYQY TNEEAVSEV
Sbjct: 601 PKWNNALLEMFRIDYVGTSPYISCLPSLYHHRLGPEDRFLILSSDGLYQYLTNEEAVSEV 660

Query: 661 ELFIALQPEGDPAQHLVELLLFRAAKRAGMDFHELLEIPQGDRRKYHDDVSIIVISLEGR 716
           ELFIAL P+GDPAQHLVE +LFRAAK+AGMDFHELLEIPQGDRR+YHDDVSIIVISLEG 
Sbjct: 661 ELFIALSPDGDPAQHLVEEVLFRAAKKAGMDFHELLEIPQGDRRRYHDDVSIIVISLEGM 720

BLAST of Cucsa.172480 vs. TrEMBL
Match: W9RU33_9ROSA (Uncharacterized protein OS=Morus notabilis GN=L484_025225 PE=4 SV=1)

HSP 1 Score: 1122.5 bits (2902), Expect = 0.0e+00
Identity = 588/744 (79.03%), Postives = 636/744 (85.48%), Query Frame = 1

Query: 1   MGNGIGKLGVCFTGINDGRRRPDISVLLTNPLDEGLGHSFCYVRPDPLRLSLSKVHSEET 60
           MGNG GKL VCFTG  D RRR DI + L +PLDEGLGHSFCYVRPDP RLS SKVHSEET
Sbjct: 1   MGNGFGKLTVCFTGGGDARRRHDIPLYLPDPLDEGLGHSFCYVRPDPTRLSSSKVHSEET 60

Query: 61  TTFRSISGASVSANTSTPLSTALIDLYSYNSIDRAAAFESSTSFASIPLQPIPRNMINSG 120
           TTF++ISGASVSANTSTPLSTALIDLYSYNSIDRAAAFESSTSFASIPLQPIPRN I SG
Sbjct: 61  TTFKTISGASVSANTSTPLSTALIDLYSYNSIDRAAAFESSTSFASIPLQPIPRNFIGSG 120

Query: 121 PISGNFSGIPCSGPLERGFLSGPIERGFMSGPLDRGIFSGPLDNNS--TSNEFQRSFSHG 180
           PIS  F GIP SGPLERGF+SGPIERGFMSGPLDRG+FSGP+D +   T+N+FQRSFS G
Sbjct: 121 PISAPF-GIPSSGPLERGFMSGPIERGFMSGPLDRGLFSGPMDKSGPDTNNQFQRSFSQG 180

Query: 181 AGFNSRSRSRKSRLIRILRTAISKSLHRGQNSIVAPIK-----PVKEPDWIVLPEKHN-- 240
            GF  R RS K +LIR LR AISK+++RGQNSIVAPIK      VKEP+W    EKHN  
Sbjct: 181 -GFAFRPRSAKGKLIRALRRAISKTINRGQNSIVAPIKGLSLSSVKEPEWNFGLEKHNHH 240

Query: 241 --ENLTVSSLNFSSEGSLDDDDSMESQNLQWAQGKAGEDRVHVVVSEEHGWVFVGIYDGF 300
             ENLTVSSLN SSEGSL+DDDS ESQNLQWAQGKAGEDRVHVVVSEEHGWVFVGIYDGF
Sbjct: 241 HNENLTVSSLNLSSEGSLEDDDSFESQNLQWAQGKAGEDRVHVVVSEEHGWVFVGIYDGF 300

Query: 301 NGPDAPDYLLSNLYSSVHKELKGLLWDEKLD--SAIVAAPATSPVQSENPLSETRD---- 360
           NGPDAPDYLLSNLYS+VHKELKGLLWDEK +  SA  AA AT P   +NP SE  D    
Sbjct: 301 NGPDAPDYLLSNLYSAVHKELKGLLWDEKFEPASAAAAAAATFP---DNPNSEAADQDSL 360

Query: 361 -------LGRNRVSD-ACSQCVEQENH-PCVSGD-GNSGLNSRKRKGKN-LRGKFRG-AA 420
                  LG N+V D AC QCVEQEN+ P  SGD GN   NSR++K ++  + K+RG A 
Sbjct: 361 PSVADSDLGGNQVGDDACFQCVEQENNLPWASGDDGNCVSNSRRKKCQSGSKSKYRGDAT 420

Query: 421 EKWEDNQRRWKCEWDRERLELDRRLKEQLNRSGSNGSRLINHSDVLKALSQALKKTEEAY 480
            KWEDNQ+RW+CEWDRERLELDRRLKEQLNRSGS+G   INHSDVLKALSQAL+KTEEAY
Sbjct: 421 NKWEDNQKRWRCEWDRERLELDRRLKEQLNRSGSDGGGAINHSDVLKALSQALRKTEEAY 480

Query: 481 LDIADKMLDENPELALMGSCVLVMLMKGDDVYVMNVGDSRAVLAQKAEPDYWLGKIRQDL 540
           LDIADKML ENPELALMGSCVLVMLMKG+DVYVMNVGDSRAVLAQK+EPDYWLGKIRQDL
Sbjct: 481 LDIADKMLVENPELALMGSCVLVMLMKGEDVYVMNVGDSRAVLAQKSEPDYWLGKIRQDL 540

Query: 541 ERINEEPVHDIEALDGDGAFTSANLTAFQLSMDHSTSVDEEVQRIKNEHPDDAGAVMNDR 600
           ERINEE +HD+E+ DG+   T A+LTA QL+ DHSTSV+EEVQRI+ EHPDD+ AV+NDR
Sbjct: 541 ERINEETLHDLESPDGERPNTIASLTALQLTKDHSTSVEEEVQRIREEHPDDSCAVINDR 600

Query: 601 VKGSLKVTRAFGAGFLKQPKWNNALLEMFRIDYVGRSPYINCLPSLYHHRLGPKDRFLIL 660
           VKGSLKVTRAFGAGFLKQPKWNNALLEMFRIDY+G SPYI C P L+HHRLGPKDRFLIL
Sbjct: 601 VKGSLKVTRAFGAGFLKQPKWNNALLEMFRIDYIGTSPYITCFPFLHHHRLGPKDRFLIL 660

Query: 661 SSDGLYQYFTNEEAVSEVELFIALQPEGDPAQHLVELLLFRAAKRAGMDFHELLEIPQGD 716
           SSDGLYQYFTNEEAVSEVELFI LQPEGDPAQHLVE +LFRAAK+AGMDFHELLEIPQGD
Sbjct: 661 SSDGLYQYFTNEEAVSEVELFITLQPEGDPAQHLVEEVLFRAAKKAGMDFHELLEIPQGD 720

BLAST of Cucsa.172480 vs. TrEMBL
Match: M5WEB7_PRUPE (Uncharacterized protein OS=Prunus persica GN=PRUPE_ppa001966mg PE=4 SV=1)

HSP 1 Score: 1102.8 bits (2851), Expect = 0.0e+00
Identity = 565/736 (76.77%), Postives = 633/736 (86.01%), Query Frame = 1

Query: 1   MGNGIGKLGVCFTGINDGR--RRPDISVLLTNPLDEGLGHSFCYVRPDPLRLSLSKVHSE 60
           MGNG GK+  CFTG  + R   R D++V+ ++  DEGLGHSFCYVRPDP  L+ SKVHSE
Sbjct: 1   MGNGFGKVSGCFTGGKERRCHGRHDVAVVASDSYDEGLGHSFCYVRPDPTWLASSKVHSE 60

Query: 61  ETTTFRSISGASVSANTSTPLSTALIDLYSYNSIDRAAAFESSTSFASIPLQPIPRNMIN 120
           E TTFR+ISGASVSANTSTPLST+L+DLYSYNSIDRAAAFESSTSFASIPLQPIPRN+IN
Sbjct: 61  EATTFRTISGASVSANTSTPLSTSLVDLYSYNSIDRAAAFESSTSFASIPLQPIPRNLIN 120

Query: 121 SGPISGNF-SGIPCSGPLERGFLSGPIERGFMSGPLDRGIFSGPLDNNSTSNEFQRSFSH 180
           SGPISGN  +G+P SGPLERGFLSGPIERGFMSGPLDRGIFSGP++    S +FQRSFS 
Sbjct: 121 SGPISGNLGAGVPGSGPLERGFLSGPIERGFMSGPLDRGIFSGPMEKGY-SEQFQRSFSI 180

Query: 181 GAGFNSRSRSRKSRLIRILRTAISKSLHRGQNSIVAPIKP----VKEPDWIVLPEK---- 240
           G G   + RS K +LIR+L+ AISK+++RGQNSIV PIK     VKE DW+V  ++    
Sbjct: 181 G-GLGFKPRSGKRKLIRVLQRAISKTIYRGQNSIVLPIKSRVVSVKE-DWVVGGQEKFHH 240

Query: 241 HNENLTVSSLNFSSEGSLDDDDSMESQNLQWAQGKAGEDRVHVVVSEEHGWVFVGIYDGF 300
           HNENLTVSS+NFSSEGSL+DD+S+ SQNLQWAQGKAGEDRVHVVVSEE GWVFVGIYDGF
Sbjct: 241 HNENLTVSSVNFSSEGSLEDDESLASQNLQWAQGKAGEDRVHVVVSEEDGWVFVGIYDGF 300

Query: 301 NGPDAPDYLLSNLYSSVHKELKGLLWDEKLDSAIVAAPATSPVQSENPLSETR-----DL 360
           NGPDAPDYLLSNLYS+V+KELKGLLWDE+ +S  VAAPATSPV  E+  S +      DL
Sbjct: 301 NGPDAPDYLLSNLYSAVNKELKGLLWDERAESGAVAAPATSPVHYEDSNSVSNMESDGDL 360

Query: 361 GRNRVSDACSQCVEQENHPCVSGDGNSGLNSRKRKGKNLRGKFRGAAEKWEDNQRRWKCE 420
           GRN + D C+QCVEQENHP  SGDGN   NSR  +G+N + K+RG A KWEDNQRRW+CE
Sbjct: 361 GRNPMVDDCAQCVEQENHPSASGDGNLDSNSRSMEGRNSKTKYRGTARKWEDNQRRWRCE 420

Query: 421 WDRERLELDRRLKEQLNRS-GSNGSRL----INHSDVLKALSQALKKTEEAYLDIADKML 480
           WDRER+ELDRRLKEQL++  GS+GS      INHSDVLKALSQAL+KTEEAYL++ADKML
Sbjct: 421 WDRERVELDRRLKEQLSKFVGSDGSSAGGATINHSDVLKALSQALRKTEEAYLEVADKML 480

Query: 481 DENPELALMGSCVLVMLMKGDDVYVMNVGDSRAVLAQKAEPDYWLGKIRQDLERINEEPV 540
            ENPELALMGSCVLVMLMKG+DVYVMNVGDSR VLAQKAEPDYWLGKIRQDLERINEE +
Sbjct: 481 LENPELALMGSCVLVMLMKGEDVYVMNVGDSRVVLAQKAEPDYWLGKIRQDLERINEETL 540

Query: 541 HDIEALDGDGAFTSANLTAFQLSMDHSTSVDEEVQRIKNEHPDDAGAVMNDRVKGSLKVT 600
           +D+EA DG+ A T  NLTA QL+MDHSTSV+EEV RI+NEHP+D  AVMNDRVKGSLKVT
Sbjct: 541 NDLEASDGERANTIPNLTAIQLTMDHSTSVEEEVHRIRNEHPEDPCAVMNDRVKGSLKVT 600

Query: 601 RAFGAGFLKQPKWNNALLEMFRIDYVGRSPYINCLPSLYHHRLGPKDRFLILSSDGLYQY 660
           RAFGAGFLKQPKWNNALLE F+IDYVG SPYI C PSL+HHRLGPKDRFL+LSSDGLYQY
Sbjct: 601 RAFGAGFLKQPKWNNALLEAFQIDYVGTSPYITCFPSLHHHRLGPKDRFLVLSSDGLYQY 660

Query: 661 FTNEEAVSEVELFIALQPEGDPAQHLVELLLFRAAKRAGMDFHELLEIPQGDRRKYHDDV 716
           FTN+EAVSEVELFI LQPEGDPAQHLVE +LFRAAK+AGMDFHELL+IPQGDRR+YHDDV
Sbjct: 661 FTNKEAVSEVELFITLQPEGDPAQHLVEEVLFRAAKKAGMDFHELLQIPQGDRRRYHDDV 720

BLAST of Cucsa.172480 vs. TrEMBL
Match: B9GJN9_POPTR (Phosphatase 2C family protein OS=Populus trichocarpa GN=POPTR_0001s24620g PE=4 SV=2)

HSP 1 Score: 1101.3 bits (2847), Expect = 0.0e+00
Identity = 555/734 (75.61%), Postives = 634/734 (86.38%), Query Frame = 1

Query: 1   MGNGIGKLGVCFTGINDGRRRPDISVLLTNPLDEGLGHSFCYVRPDPLRLSLSKVHSEET 60
           MGNG GKL VCFTG  + RRR  +S L+++PLDEGLGHSFCYVRPDP RLS SKVHSEET
Sbjct: 1   MGNGFGKLTVCFTGDGEARRRKVLSELISDPLDEGLGHSFCYVRPDPNRLSSSKVHSEET 60

Query: 61  TTFRSISGASVSANTSTPLSTALIDLYSYNSIDRAAAFESSTSFASIPLQPIPRNM---I 120
           TTFR+ISGASVSANTSTPLSTA ID Y YN+IDRAA+FESSTSFASIPLQPIPR++   +
Sbjct: 61  TTFRTISGASVSANTSTPLSTAFIDPYVYNTIDRAASFESSTSFASIPLQPIPRSLFGSL 120

Query: 121 NSGPISGNFSGIPCSGPLERGFLSGPIERGFMSGPLDRGIFSGPLDNNSTSNEFQRSFSH 180
           NSGP++GN + +PCSGP+ERGFLSGPIERGFMSGPLDRG+FSGPL+  S S++FQRSFSH
Sbjct: 121 NSGPLTGNSALMPCSGPMERGFLSGPIERGFMSGPLDRGLFSGPLEKGS-SDQFQRSFSH 180

Query: 181 GAGFNSRSRSRKSRLIRILRTAISKSLHRGQNSIVAPIKP----VKEPDWIVLPEKHNEN 240
           G GF  RSRS K  LIR+L+ AI K++ RG NSIVAPIK     VKEP+WI+  EK NE 
Sbjct: 181 G-GFAFRSRSGKRSLIRVLQRAICKTITRGHNSIVAPIKGGVGVVKEPEWILSSEKQNE- 240

Query: 241 LTVSSLNFSSEGSLDDDDSMESQNLQWAQGKAGEDRVHVVVSEEHGWVFVGIYDGFNGPD 300
           LTVSS+N SS+GSL+DDDS+ESQNLQWAQGKAGEDRVHVVVSEEHGWVFVGIYDGFNGPD
Sbjct: 241 LTVSSVNLSSDGSLEDDDSLESQNLQWAQGKAGEDRVHVVVSEEHGWVFVGIYDGFNGPD 300

Query: 301 APDYLLSNLYSSVHKELKGLLWDEKLDSAIVAAPATSPVQSE--NPLSETRDLGRNRVSD 360
           APDYLLSNLYS+VHKELKGLLWD+K +   +++ A+SP QSE  N +  T     N + +
Sbjct: 301 APDYLLSNLYSAVHKELKGLLWDDKFEPDQISSHASSPFQSETSNSVESTNSRLENVIHN 360

Query: 361 A----------CSQCVEQENHPCVSGDGNSGLNSRKRKGKNLRGKFRGAAEKWEDNQRRW 420
           +          C Q ++Q+NHPC SGD    ++ R+++ ++ +GK+RGAA+KWE+NQRRW
Sbjct: 361 SETNGNCRNYECYQYLDQDNHPCPSGDAGFDMSLRRKRSRSSKGKYRGAAKKWEENQRRW 420

Query: 421 KCEWDRERLELDRRLKEQLNRSGSNGSRLINHSDVLKALSQALKKTEEAYLDIADKMLDE 480
           KCEWDRER+ELD+RLKEQLNRSGS+ S  INH+DVLKALSQALKKTEE+YL+IADKML E
Sbjct: 421 KCEWDRERMELDKRLKEQLNRSGSDASP-INHADVLKALSQALKKTEESYLEIADKMLVE 480

Query: 481 NPELALMGSCVLVMLMKGDDVYVMNVGDSRAVLAQKAEPDYWLGKIRQDLERINEEPVHD 540
           NPEL+LMGSCVLVMLMKG+DVYVMNVGDSRAVLAQKAEPDYWLGKIRQDLERINEE +HD
Sbjct: 481 NPELSLMGSCVLVMLMKGEDVYVMNVGDSRAVLAQKAEPDYWLGKIRQDLERINEETLHD 540

Query: 541 IEALDGDGAFTSANLTAFQLSMDHSTSVDEEVQRIKNEHPDDAGAVMNDRVKGSLKVTRA 600
           +EA D + + +   LTA QLS+DHSTSV+EEVQRIK EHPDDA AV+NDRVKGSLKVTRA
Sbjct: 541 LEAFDAERSNSMPVLTASQLSVDHSTSVEEEVQRIKKEHPDDACAVVNDRVKGSLKVTRA 600

Query: 601 FGAGFLKQPKWNNALLEMFRIDYVGRSPYINCLPSLYHHRLGPKDRFLILSSDGLYQYFT 660
           FGAGFLKQP+WNNALLEMFRIDY+G SPY+ CLPSLYHHRLGPKDRFLILSSDGLYQY T
Sbjct: 601 FGAGFLKQPRWNNALLEMFRIDYIGNSPYVTCLPSLYHHRLGPKDRFLILSSDGLYQYLT 660

Query: 661 NEEAVSEVELFIALQPEGDPAQHLVELLLFRAAKRAGMDFHELLEIPQGDRRKYHDDVSI 716
           N+EAV EVELFI LQPEGDPAQHLVE +LFRAAK+AGMDFHELL+IPQGDRR+YHDDVSI
Sbjct: 661 NDEAVYEVELFITLQPEGDPAQHLVEEVLFRAAKKAGMDFHELLDIPQGDRRRYHDDVSI 720

BLAST of Cucsa.172480 vs. TAIR10
Match: AT1G07630.1 (AT1G07630.1 pol-like 5)

HSP 1 Score: 880.6 bits (2274), Expect = 6.7e-256
Identity = 483/741 (65.18%), Postives = 558/741 (75.30%), Query Frame = 1

Query: 1   MGNGIGKLGVCFTGINDGRRRP-DISVLLTNPLDEGLGHSFCYVRPDPLRLSLSKVHSEE 60
           MGNG+ KL +CFTG    R RP DISVLL +PLDEGLGHSFCYVRPDP  +S SKVHSEE
Sbjct: 1   MGNGVTKLSICFTGGGGERLRPKDISVLLPDPLDEGLGHSFCYVRPDPTLISSSKVHSEE 60

Query: 61  ---TTTFRSISGASVSANTSTPLSTALIDLYSYNSIDRAAAFESSTSFASIPLQPIPRNM 120
              TTTFR+ISGASVSANT+TPLST+L D Y +  IDRAAAFES+TSF+SIPLQPIP++ 
Sbjct: 61  DTTTTTFRTISGASVSANTATPLSTSLYDPYGH--IDRAAAFESTTSFSSIPLQPIPKS- 120

Query: 121 INSGPISGNFSGIPCSGPLERGFLSGPIERGFMSGPLDR-GIFSGPLD--NNSTSNEFQR 180
             SGPI      +  SGP+ERGFLSGPIERGFMSGPLDR G+FSGPLD  N+   ++FQR
Sbjct: 121 --SGPI------VLGSGPIERGFLSGPIERGFMSGPLDRVGLFSGPLDKPNSDHHHQFQR 180

Query: 181 SFSHGAGFNSRSRSRKSRLIRILRTAISKSLHRGQNSIVAPIKPVKEPD-WIVLPEK--- 240
           SFSHG     R  SRK  L+RILR AISK++ RGQNSIVAPIK VK+ D W +  EK   
Sbjct: 181 SFSHGLAL--RVGSRKRSLVRILRRAISKTMSRGQNSIVAPIKSVKDSDNWGIRSEKSRN 240

Query: 241 -HNENLTVSSLNFSSEGSLDDDDSMESQNLQWAQGKAGEDRVHVVVSEEHGWVFVGIYDG 300
            HNENLTV+SLNFSSE SLDDD S+E+QNLQWAQGKAGEDRVHVVVSEEHGW+FVGIYDG
Sbjct: 241 LHNENLTVNSLNFSSEVSLDDDVSLENQNLQWAQGKAGEDRVHVVVSEEHGWLFVGIYDG 300

Query: 301 FNGPDAPDYLLSNLYSSVHKELKGLLWDEKLDSAIVAAPATSPVQSENPLSETRDLGRNR 360
           FNGPDAPDYLLS+LY  VH+ELKGLLWD+                  N  S+++DL R+ 
Sbjct: 301 FNGPDAPDYLLSHLYPVVHRELKGLLWDD-----------------SNVESKSQDLERSN 360

Query: 361 VSDACSQCVEQENHPCVSGDGNSGLNSRKRKGKNLRGKFRGAAEKW---------EDNQR 420
             ++CS   ++++  C                           E+W         +D  R
Sbjct: 361 GDESCSN--QEKDETC---------------------------ERWWRCEWDRESQDLDR 420

Query: 421 RWKCEWDR----ERLELDRRLKEQLNRSGSNGSRLINHSDVLKALSQALKKTEEAYLDIA 480
           R K +  R    +RL     + E L+++                     +KTEEAYLD A
Sbjct: 421 RLKEQISRRSGSDRLTNHSEVLEALSQAL--------------------RKTEEAYLDTA 480

Query: 481 DKMLDENPELALMGSCVLVMLMKGDDVYVMNVGDSRAVLAQKAEPDYWLGKIRQDLERIN 540
           DKMLDENPELALMGSCVLVMLMKG+D+YVMNVGDSRAVL QK+EPDYWL KIRQDLERIN
Sbjct: 481 DKMLDENPELALMGSCVLVMLMKGEDIYVMNVGDSRAVLGQKSEPDYWLAKIRQDLERIN 540

Query: 541 EEP-VHDIEALDGDGAFTSANLTAFQLSMDHSTSVDEEVQRIKNEHPDDAGAVMNDRVKG 600
           EE  ++D+E  +GD +    NL+AFQL++DHST+++EEV+RI+NEHPDD  AV N+RVKG
Sbjct: 541 EETMMNDLEGCEGDQSSLVPNLSAFQLTVDHSTNIEEEVERIRNEHPDDVTAVTNERVKG 600

Query: 601 SLKVTRAFGAGFLKQPKWNNALLEMFRIDYVGRSPYINCLPSLYHHRLGPKDRFLILSSD 660
           SLKVTRAFGAGFLKQPKWNNALLEMF+IDYVG+SPYINCLPSLYHHRLG KDRFLILSSD
Sbjct: 601 SLKVTRAFGAGFLKQPKWNNALLEMFQIDYVGKSPYINCLPSLYHHRLGSKDRFLILSSD 660

Query: 661 GLYQYFTNEEAVSEVELFIALQPEGDPAQHLVELLLFRAAKRAGMDFHELLEIPQGDRRK 716
           GLYQYFTNEEAVSEVELFI LQPEGDPAQHLV+ LLFRAAK+AGMDFHELLEIPQG+RR+
Sbjct: 661 GLYQYFTNEEAVSEVELFITLQPEGDPAQHLVQELLFRAAKKAGMDFHELLEIPQGERRR 662

BLAST of Cucsa.172480 vs. TAIR10
Match: AT2G28890.1 (AT2G28890.1 poltergeist like 4)

HSP 1 Score: 825.5 bits (2131), Expect = 2.6e-239
Identity = 452/735 (61.50%), Postives = 526/735 (71.56%), Query Frame = 1

Query: 1   MGNGIGKLGVCFTGINDGRRRPDISVLLTNPLDEGLGHSFCYVRPDPLRLSLSKVHSEE- 60
           MGNGIGKL  C TG     ++P++S+L  +PLDEGLGHSFCYVRPDP R+S SKVHSEE 
Sbjct: 1   MGNGIGKLSKCLTGGAGRNKKPELSILEPDPLDEGLGHSFCYVRPDPTRVSSSKVHSEEE 60

Query: 61  TTTFRSISGASVSANTSTPLSTALIDLYSYNSIDRAAAFESSTSFASIPLQPIPRNMINS 120
           TTTFR+ISGASVSANT+TPLST+L D Y +  IDRAAAFES+TSF+SIPLQPIPR+   S
Sbjct: 61  TTTFRTISGASVSANTATPLSTSLYDPYGH--IDRAAAFESTTSFSSIPLQPIPRS---S 120

Query: 121 GPISGNFSGIPCSGPLERGFLSGPIERGFMSGPLDRGIFSGPLDNNSTSNEFQRSFSHGA 180
           GPI      +P SGPLERGFLSGPIERGFMSGPLD    SGP+D  + S++FQRSFSHG 
Sbjct: 121 GPI------VPGSGPLERGFLSGPIERGFMSGPLDGS--SGPIDGKTGSDQFQRSFSHGL 180

Query: 181 GFNSRSRSRKSRLIRILRTAISKSLHRGQNSIVAPIKPVKEPDWIVLPEKHN-----ENL 240
             N R  SRK  L+R+LR AISK++ RGQNSIVAPIKPVKEPDW+   +K        NL
Sbjct: 181 A-NLRVGSRKGSLVRVLRRAISKTITRGQNSIVAPIKPVKEPDWVFGSDKTRIHQIENNL 240

Query: 241 TVSSLNFSSEGSL-DDDDSMESQNLQWAQGKAGEDRVHVVVSEEHGWVFVGIYDGFNGPD 300
           TV+SLNFSSEGSL DDD S+ESQNLQWAQGKAGEDRVHVVVSEEHGW+FVGIYDGFNGPD
Sbjct: 241 TVNSLNFSSEGSLLDDDVSLESQNLQWAQGKAGEDRVHVVVSEEHGWLFVGIYDGFNGPD 300

Query: 301 APDYLLSNLYSSVHKELKGLLWDEKLDSAIVAAPATSPVQSENPLSETRDLGRNRVSDAC 360
           APDYLLS+LY +VH+ELKGLLWD+         P T    S+    E RD          
Sbjct: 301 APDYLLSHLYPAVHRELKGLLWDD---------PKTDAKSSDEADVENRD---------- 360

Query: 361 SQCVEQENHPCVSGDGNSGLNSRKRKGKNLRGKFRGAAEKWE--------DNQRRWKCEW 420
                               +S ++K KN     R    +W+        D       + 
Sbjct: 361 --------------------SSSEKKSKNWEESQRRWRCEWDRDLDRLLKDRSNGLDLDP 420

Query: 421 DRERLELDRRLKEQLNRSG----SNGSRLINHSDVLKALSQALKKTEEAYLDIADKMLDE 480
           D    ++ + L + L ++      N   +++ +  L  +                     
Sbjct: 421 DPNSSDVLKALSQALRKTEEAYLENADMMLDENPELALMGS------------------- 480

Query: 481 NPELALMGSCVLVMLMKGDDVYVMNVGDSRAVLAQKAEPDYWLGKIRQDLERINEEPVHD 540
                    CVLVMLMKG+DVY+MNVGDSRAVL QKAE DYW+GKI+QDLERINEE ++D
Sbjct: 481 ---------CVLVMLMKGEDVYLMNVGDSRAVLGQKAESDYWIGKIKQDLERINEETMND 540

Query: 541 IEAL-DGDGAFTSANLTAFQLSMDHSTSVDEEVQRIKNEHPDDAGAVMNDRVKGSLKVTR 600
            +   DG+GA     L+AFQL++DHST+V+EEV RI+ EHPDDA AV N+RVKGSLKVTR
Sbjct: 541 FDGCGDGEGASLVPTLSAFQLTVDHSTNVEEEVNRIRKEHPDDASAVSNERVKGSLKVTR 600

Query: 601 AFGAGFLKQPKWNNALLEMFRIDYVGRSPYINCLPSLYHHRLGPKDRFLILSSDGLYQYF 660
           AFGAGFLKQPKWNNALLEMF+IDY G SPYINCLPSLYHHRLG KD+FLILSSDGLYQYF
Sbjct: 601 AFGAGFLKQPKWNNALLEMFQIDYKGTSPYINCLPSLYHHRLGSKDQFLILSSDGLYQYF 654

Query: 661 TNEEAVSEVELFIALQPEGDPAQHLVELLLFRAAKRAGMDFHELLEIPQGDRRKYHDDVS 716
           TNEEAVSEVELFI LQPEGDPAQHLV+ LLFRAAK+AGMDFHELLEIPQG+RR+YHDDVS
Sbjct: 661 TNEEAVSEVELFITLQPEGDPAQHLVQELLFRAAKKAGMDFHELLEIPQGERRRYHDDVS 654

BLAST of Cucsa.172480 vs. TAIR10
Match: AT5G02400.1 (AT5G02400.1 pol-like 2)

HSP 1 Score: 622.9 bits (1605), Expect = 2.5e-178
Identity = 371/733 (50.61%), Postives = 478/733 (65.21%), Query Frame = 1

Query: 1   MGNGIGKLGVCFTGINDGR--RRPDISVLLTNPLDEGLGHSFCYVRPDPLRLSLSKVHSE 60
           MGNG+  L  C TG   G   RR D+S++      +GLGHSFCY+RPD   + L    S 
Sbjct: 1   MGNGVTTLTGCCTGTLAGEISRRYDVSLV-----HDGLGHSFCYIRPDLPGVVLPSPESP 60

Query: 61  ------ETTTFRSISGASVSANTSTPLSTALIDLYS--YNSIDRAAAFESSTSFASIPLQ 120
                 + TTFRSISGASVSAN ST LS AL       Y+S   A+AFESS +FAS+PLQ
Sbjct: 61  LRSDHIQETTFRSISGASVSANPSTALSGALSSDSDCPYSSAVSASAFESSGNFASLPLQ 120

Query: 121 PIPRNMI-NSGPISGNFSGIPCSGPLERGFLSGPIERGFMSGPLDRGIFSGPLDNNSTSN 180
           P+PR     SGPI             E G  S P ER F+SGP++ G++SGP+++   + 
Sbjct: 121 PVPRGSTWQSGPIVN-----------ESGLGSAPFERRFLSGPIESGLYSGPIESTKKTE 180

Query: 181 EFQRSFSHGAGFNSRSRSRKSRLI--RILRTAISKSLH-RGQNSIVAPIKPVKEPDWIVL 240
           + +           + +S+K+ L    +    IS +   R + S++ PI      D   L
Sbjct: 181 KEKPK-----KIRKKPKSKKNFLTFKTLFANLISNNNKPRLKKSVIEPINGSDSSDSGRL 240

Query: 241 PEKHNENLTVSSLNFSSEGSLDDDDSMESQN----LQWAQGKAGEDRVHVVVSEEHGWVF 300
              H   +T S  N + +  L+++D  +S N    +QWAQGKAGEDRVHVVVSE++GWVF
Sbjct: 241 --HHEPVITSSRSNENPKSDLEEEDEKQSMNSVLDVQWAQGKAGEDRVHVVVSEDNGWVF 300

Query: 301 VGIYDGFNGPDAPDYLLSNLYSSVHKELKGLLW-DEKLDSAIVAAPATSPVQSENPLSET 360
           VGIYDGF+GPDAPDYLL+NLY++V KEL GLLW DEKL S             EN +++T
Sbjct: 301 VGIYDGFSGPDAPDYLLNNLYTAVQKELNGLLWNDEKLRSL-----------GENGMTKT 360

Query: 361 RDLGRNRVSDACSQCVEQENHPCVSGDGNSGLNSRKRKGKNLRGKFRGAAEKWEDNQRRW 420
                 + SD       +EN P ++ D ++  +  + + K+L+                W
Sbjct: 361 -----GKCSDEEDPESGKENCPVINND-DAVASGARNQAKSLK----------------W 420

Query: 421 KCEWDRERLELDRRLKEQLNRSGSNGSRLINHSDVLKALSQALKKTEEAYLDIADKMLDE 480
           +CEW+++     +    + ++ GSN S   NH DVLKAL QAL+KTE+AYL++AD+M+ E
Sbjct: 421 RCEWEKKSNNKTKS-DNRCDQKGSN-STTTNHKDVLKALLQALRKTEDAYLELADQMVKE 480

Query: 481 NPELALMGSCVLVMLMKGDDVYVMNVGDSRAVLAQKAEPDYWLGKIRQ-DLERINEEPVH 540
           NPELALMGSCVLV LMKG+DVYVMNVGDSRAVL +K  P+   G+ RQ +LERI E+   
Sbjct: 481 NPELALMGSCVLVTLMKGEDVYVMNVGDSRAVLGRK--PNLATGRKRQKELERIREDSSL 540

Query: 541 DIEALDGDGAFTSANLTAFQLSMDHSTSVDEEVQRIKNEHPDDAGAVMNDRVKGSLKVTR 600
           + + +  +GA  +  L   QL+M+HST ++EEV+RIK EHPDD  AV NDRVKG LKVTR
Sbjct: 541 EDKEILMNGAMRNT-LVPLQLNMEHSTRIEEEVRRIKKEHPDDDCAVENDRVKGYLKVTR 600

Query: 601 AFGAGFLKQPKWNNALLEMFRIDYVGRSPYINCLPSLYHHRLGPKDRFLILSSDGLYQYF 660
           AFGAGFLKQPKWN+ALLEMFRIDY+G SPYI C PSL HH+L  +D+FLILSSDGLY+YF
Sbjct: 601 AFGAGFLKQPKWNDALLEMFRIDYIGTSPYITCSPSLCHHKLTSRDKFLILSSDGLYEYF 660

Query: 661 TNEEAVSEVELFIALQPEGDPAQHLVELLLFRAAKRAGMDFHELLEIPQGDRRKYHDDVS 714
           +N+EA+ EVE FI+  PEGDPAQHL++ +L RAA + GMDFHELLEIPQGDRR+YHDDVS
Sbjct: 661 SNQEAIFEVESFISAFPEGDPAQHLIQEVLLRAANKFGMDFHELLEIPQGDRRRYHDDVS 672

BLAST of Cucsa.172480 vs. TAIR10
Match: AT3G09400.1 (AT3G09400.1 pol-like 3)

HSP 1 Score: 573.9 bits (1478), Expect = 1.3e-163
Identity = 353/737 (47.90%), Postives = 452/737 (61.33%), Query Frame = 1

Query: 1   MGNGIGKLGVCFTGINDGRRRPDIS---VLLTNPLDEGLGHSFCYVRPDPLRLSLSKVHS 60
           MGNG+     C  G   G    +IS   V     + E LGHSFCYVRP  L  S S    
Sbjct: 1   MGNGVASFSGCCAGTTAG----EISGRYVTGVGLVQENLGHSFCYVRP-VLTGSKSSFPP 60

Query: 61  EE----------TTTFRSISGASVSANTSTPLSTALIDLYSYNSIDRAAAFESSTSFASI 120
           E           TTTFRSISGASVSANTST LST+L    S ++   A+AFESS  FAS+
Sbjct: 61  EPPLRPDPIPGTTTTFRSISGASVSANTSTALSTSL----STDTSGIASAFESSNRFASL 120

Query: 121 PLQPIPRNMINSGPISGNFSGIPCSGPLERGFLSGPIERGFMSGPLDRGIFSGPLDNNST 180
           PLQP+PR+     PI  +  G   SG  ER FLSGPIE G +SG                
Sbjct: 121 PLQPVPRS-----PIKKSDHG---SGLFERRFLSGPIESGLVSG--------------KK 180

Query: 181 SNEFQRSFSHGAGFNSRSRSRKSRLIRILRTAISKSLHRGQNSIVAPIKPVKEPDWI--- 240
           + E  +    G+   ++ + +KS         +  +L   + S++ PI      D     
Sbjct: 181 TKEKAKLKKSGSKSFTKPKLKKSESKIFTFKNVFTNLSCSKKSVIKPINGFDSFDGSSDT 240

Query: 241 --VLPEKHNENLTVSSLNFSSEGSLDD--DDSMESQNLQWAQGKAGEDRVHVVVSEEHGW 300
              +PE ++ +  VSS         +D  + ++E   +QWAQGKAGEDRVHV++SEE+GW
Sbjct: 241 DRYIPEINSLSTIVSSHEKPRIKEEEDKTESALEEPKIQWAQGKAGEDRVHVILSEENGW 300

Query: 301 VFVGIYDGFNGPDAPDYLLSNLYSSVHKELKGLLWDEKLDSAIVAAPATSPVQSENPLSE 360
           +FVGIYDGF+GPD PDYL+ NLY++V +ELKGLLW +K +S        S ++ ++ +  
Sbjct: 301 LFVGIYDGFSGPDPPDYLIKNLYTAVLRELKGLLWIDKGES--YNRNGESNIEKQSTVEH 360

Query: 361 TRDLGRNRVSDACSQCVEQENHPCVSGD----GNSGLNSRKRKGKNLRGKFRGAAEKWED 420
             D              +QEN P ++G+    G+  + S  +K                 
Sbjct: 361 ASDS-------------DQENCPVMNGNDVACGSRNITSDVKK----------------- 420

Query: 421 NQRRWKCEWDRERLELDRRLKEQLNRSGSNGSRLINHSDVLKALSQALKKTEEAYLDIAD 480
              +W+CEW+                + SN S  INH DVL+AL QAL+KTEE++    D
Sbjct: 421 --LQWRCEWEH---------------NSSNKSNNINHKDVLRALQQALEKTEESF----D 480

Query: 481 KMLDENPELALMGSCVLVMLMKGDDVYVMNVGDSRAVLAQKAEPDYWLGKIRQDLERINE 540
            M++ENPELALMGSCVLV LMKG+DVYVM+VGDSRAVLA++  P+    K++++LER+ E
Sbjct: 481 LMVNENPELALMGSCVLVTLMKGEDVYVMSVGDSRAVLARR--PNVEKMKMQKELERVKE 540

Query: 541 EPVHDIEALDGDGAFTSANLTAFQLSMDHSTSVDEEVQRIKNEHPDDAGAVMNDRVKGSL 600
           E   +   +   G    + L   QL+ +HSTSV+EEV+RIK EHPDD  A+ N+RVKG L
Sbjct: 541 ESPLETLFITERGL---SLLVPVQLNKEHSTSVEEEVRRIKKEHPDDILAIENNRVKGYL 600

Query: 601 KVTRAFGAGFLKQPKWNNALLEMFRIDYVGRSPYINCLPSLYHHRLGPKDRFLILSSDGL 660
           KVTRAFGAGFLKQPKWN ALLEMFRIDYVG SPYI C PSL+HHRL  +D+FLILSSDGL
Sbjct: 601 KVTRAFGAGFLKQPKWNEALLEMFRIDYVGTSPYITCSPSLHHHRLSSRDKFLILSSDGL 648

Query: 661 YQYFTNEEAVSEVELFIALQPEGDPAQHLVELLLFRAAKRAGMDFHELLEIPQGDRRKYH 714
           Y+YF+NEEA+ EV+ FI+  PEGDPAQHL++ +L RAAK+ GMDFHELLEIPQGDRR+YH
Sbjct: 661 YEYFSNEEAIFEVDSFISAFPEGDPAQHLIQEVLLRAAKKYGMDFHELLEIPQGDRRRYH 648

BLAST of Cucsa.172480 vs. TAIR10
Match: AT2G46920.1 (AT2G46920.1 Protein phosphatase 2C family protein)

HSP 1 Score: 352.8 bits (904), Expect = 4.9e-97
Identity = 196/388 (50.52%), Postives = 261/388 (67.27%), Query Frame = 1

Query: 361 NHPCVS------GDGNSGLNSRKRKGKNLRGKFRGAAEKWEDNQRR---WKCEWDRER-L 420
           N PC +      G  NSG     +K + +  K R   +K +  +++   W  +W RE  +
Sbjct: 456 NDPCTTDRSALDGIPNSGQRHGTKKSQ-ISSKIRRMYQKQKSLRKKLFPWSYDWHREEGI 515

Query: 421 ELDRRLKEQLNRSGSNGSRLINHSDVLKALSQALKKTEEAYLDIADKMLDENPELALMGS 480
            ++ ++ E         S  ++H  VL+A+++AL+ TEEAY+D+ +K LD NPELALMGS
Sbjct: 516 CVEEKIVESSGPIRRRWSGTVDHDAVLRAMARALESTEEAYMDMVEKSLDINPELALMGS 575

Query: 481 CVLVMLMKGDDVYVMNVGDSRAVLAQK------AEP----DYWLGK--------IRQDLE 540
           CVLVMLMK  DVYVMNVGDSRA+LAQ+      + P    D  +G         +R +L+
Sbjct: 576 CVLVMLMKDQDVYVMNVGDSRAILAQERLHDRHSNPGFGNDEGIGHKSRSRESLVRIELD 635

Query: 541 RINEE-PVHD----IEALDGDGAFTSANLT--AFQLSMDHSTSVDEEVQRIKNEHPDDAG 600
           RI+EE P+H+    I   + +   TS  L   A QLS DHSTSV+EE+ RI++EHP+D  
Sbjct: 636 RISEESPIHNQATPISVSNKNRDVTSYRLKMRAVQLSSDHSTSVEEEIWRIRSEHPEDDQ 695

Query: 601 AVMNDRVKGSLKVTRAFGAGFLKQPKWNNALLEMFRIDYVGRSPYINCLPSLYHHRLGPK 660
           +++ DRVKG LKVTRAFGAGFLK+P +N ALLEMF+++Y+G  PYI C P   HHRL   
Sbjct: 696 SILKDRVKGQLKVTRAFGAGFLKKPNFNEALLEMFQVEYIGTDPYITCEPCTVHHRLTSS 755

Query: 661 DRFLILSSDGLYQYFTNEEAVSEVELFIALQPEGDPAQHLVELLLFRAAKRAGMDFHELL 714
           DRF++LSSDGLY+YF+NEE V+ V  FI   PEGDPAQ+L+  LL RAA + GM+FH+LL
Sbjct: 756 DRFMVLSSDGLYEYFSNEEVVAHVTWFIENVPEGDPAQYLIAELLSRAATKNGMEFHDLL 815

HSP 2 Score: 225.7 bits (574), Expect = 9.0e-59
Identity = 168/463 (36.29%), Postives = 234/463 (50.54%), Query Frame = 1

Query: 1   MGNGIGKLGVCFTGINDGRRRPDISVLLTNPLDEGLGHSFC------------------- 60
           MGNG  ++  CF   ND   +  + +    PLDEGLGHSFC                   
Sbjct: 1   MGNGTSRVVGCFVPSND---KNGVDLEFLEPLDEGLGHSFCYVRPSIFESPDITPSNSER 60

Query: 61  -------------------YVRPDPLRLSLSKVHSEETTTFRSISGASVSANTSTPLSTA 120
                               +  DP  L+         TTF++ISGASVSAN ST  +  
Sbjct: 61  FTIDSSTIDSETLTGSFRNDIVDDPSFLNRHNSKGLAETTFKAISGASVSANVSTARTGN 120

Query: 121 LIDLYSYNSIDRAAAFESSTSFASIPLQPIPRNMINSGPISGNFSGIPCSGPLERGFLSG 180
            + L S + ++ AA+FES++SFASIPLQP+PR    SGP++G  SG     PLERGF SG
Sbjct: 121 QMALCSSDVLEPAASFESTSSFASIPLQPLPRG--GSGPLNGFMSG-----PLERGFASG 180

Query: 181 PIER--GFMSGPLDRGIFSGPLDNNSTSNEFQRSFSHGAGFNSRSRSRKSRLIRILRTAI 240
           P++R  GFMSGP+++G+ SGPLD +  SN     FS    F  R + R  R +R +   +
Sbjct: 181 PLDRNNGFMSGPIEKGVMSGPLDVSDRSN-----FSAPLSFR-RKKPRFQRFMRSVSGPM 240

Query: 241 SKSLHRGQNSIVAPIKPVKEPDWIVLPEKHNENLTVSSLNFSSEGSL---DDDDSMES-Q 300
             +L R  +     +       W+     H E  T  S     +G L   D +  +ES +
Sbjct: 241 KSTLARTFSRRSGGLS------WMHRFFLHPE--TRVSWAVGKDGKLHGEDPESCLESNR 300

Query: 301 NLQWAQGKAGEDRVHVVVSEEHGWVFVGIYDGFNGPDAPDYLLSNLYSSVHKELKGLLWD 360
           NLQWA GKAGEDRVHVV+SEE GW+F+GIYDGF+GPDAPD+++S+LY ++ KEL+GLLWD
Sbjct: 301 NLQWAHGKAGEDRVHVVLSEEQGWLFIGIYDGFSGPDAPDFVMSHLYKAIDKELEGLLWD 360

Query: 361 -EKLDSAIVAAPATSPVQSENPLSETRDLGRNRVSDACSQCVEQENHPCVSGDGNSGLNS 415
            E+        P   P   EN            +S+  S+ V  E+   +  D +S  N+
Sbjct: 361 YEEPSEDNQLQPDQEPPTEENMCDP------ESISEQHSKSVVAESEEVMIDDISSLGNT 420

BLAST of Cucsa.172480 vs. NCBI nr
Match: gi|449450726|ref|XP_004143113.1| (PREDICTED: probable protein phosphatase 2C 4 [Cucumis sativus])

HSP 1 Score: 1441.8 bits (3731), Expect = 0.0e+00
Identity = 715/715 (100.00%), Postives = 715/715 (100.00%), Query Frame = 1

Query: 1   MGNGIGKLGVCFTGINDGRRRPDISVLLTNPLDEGLGHSFCYVRPDPLRLSLSKVHSEET 60
           MGNGIGKLGVCFTGINDGRRRPDISVLLTNPLDEGLGHSFCYVRPDPLRLSLSKVHSEET
Sbjct: 1   MGNGIGKLGVCFTGINDGRRRPDISVLLTNPLDEGLGHSFCYVRPDPLRLSLSKVHSEET 60

Query: 61  TTFRSISGASVSANTSTPLSTALIDLYSYNSIDRAAAFESSTSFASIPLQPIPRNMINSG 120
           TTFRSISGASVSANTSTPLSTALIDLYSYNSIDRAAAFESSTSFASIPLQPIPRNMINSG
Sbjct: 61  TTFRSISGASVSANTSTPLSTALIDLYSYNSIDRAAAFESSTSFASIPLQPIPRNMINSG 120

Query: 121 PISGNFSGIPCSGPLERGFLSGPIERGFMSGPLDRGIFSGPLDNNSTSNEFQRSFSHGAG 180
           PISGNFSGIPCSGPLERGFLSGPIERGFMSGPLDRGIFSGPLDNNSTSNEFQRSFSHGAG
Sbjct: 121 PISGNFSGIPCSGPLERGFLSGPIERGFMSGPLDRGIFSGPLDNNSTSNEFQRSFSHGAG 180

Query: 181 FNSRSRSRKSRLIRILRTAISKSLHRGQNSIVAPIKPVKEPDWIVLPEKHNENLTVSSLN 240
           FNSRSRSRKSRLIRILRTAISKSLHRGQNSIVAPIKPVKEPDWIVLPEKHNENLTVSSLN
Sbjct: 181 FNSRSRSRKSRLIRILRTAISKSLHRGQNSIVAPIKPVKEPDWIVLPEKHNENLTVSSLN 240

Query: 241 FSSEGSLDDDDSMESQNLQWAQGKAGEDRVHVVVSEEHGWVFVGIYDGFNGPDAPDYLLS 300
           FSSEGSLDDDDSMESQNLQWAQGKAGEDRVHVVVSEEHGWVFVGIYDGFNGPDAPDYLLS
Sbjct: 241 FSSEGSLDDDDSMESQNLQWAQGKAGEDRVHVVVSEEHGWVFVGIYDGFNGPDAPDYLLS 300

Query: 301 NLYSSVHKELKGLLWDEKLDSAIVAAPATSPVQSENPLSETRDLGRNRVSDACSQCVEQE 360
           NLYSSVHKELKGLLWDEKLDSAIVAAPATSPVQSENPLSETRDLGRNRVSDACSQCVEQE
Sbjct: 301 NLYSSVHKELKGLLWDEKLDSAIVAAPATSPVQSENPLSETRDLGRNRVSDACSQCVEQE 360

Query: 361 NHPCVSGDGNSGLNSRKRKGKNLRGKFRGAAEKWEDNQRRWKCEWDRERLELDRRLKEQL 420
           NHPCVSGDGNSGLNSRKRKGKNLRGKFRGAAEKWEDNQRRWKCEWDRERLELDRRLKEQL
Sbjct: 361 NHPCVSGDGNSGLNSRKRKGKNLRGKFRGAAEKWEDNQRRWKCEWDRERLELDRRLKEQL 420

Query: 421 NRSGSNGSRLINHSDVLKALSQALKKTEEAYLDIADKMLDENPELALMGSCVLVMLMKGD 480
           NRSGSNGSRLINHSDVLKALSQALKKTEEAYLDIADKMLDENPELALMGSCVLVMLMKGD
Sbjct: 421 NRSGSNGSRLINHSDVLKALSQALKKTEEAYLDIADKMLDENPELALMGSCVLVMLMKGD 480

Query: 481 DVYVMNVGDSRAVLAQKAEPDYWLGKIRQDLERINEEPVHDIEALDGDGAFTSANLTAFQ 540
           DVYVMNVGDSRAVLAQKAEPDYWLGKIRQDLERINEEPVHDIEALDGDGAFTSANLTAFQ
Sbjct: 481 DVYVMNVGDSRAVLAQKAEPDYWLGKIRQDLERINEEPVHDIEALDGDGAFTSANLTAFQ 540

Query: 541 LSMDHSTSVDEEVQRIKNEHPDDAGAVMNDRVKGSLKVTRAFGAGFLKQPKWNNALLEMF 600
           LSMDHSTSVDEEVQRIKNEHPDDAGAVMNDRVKGSLKVTRAFGAGFLKQPKWNNALLEMF
Sbjct: 541 LSMDHSTSVDEEVQRIKNEHPDDAGAVMNDRVKGSLKVTRAFGAGFLKQPKWNNALLEMF 600

Query: 601 RIDYVGRSPYINCLPSLYHHRLGPKDRFLILSSDGLYQYFTNEEAVSEVELFIALQPEGD 660
           RIDYVGRSPYINCLPSLYHHRLGPKDRFLILSSDGLYQYFTNEEAVSEVELFIALQPEGD
Sbjct: 601 RIDYVGRSPYINCLPSLYHHRLGPKDRFLILSSDGLYQYFTNEEAVSEVELFIALQPEGD 660

Query: 661 PAQHLVELLLFRAAKRAGMDFHELLEIPQGDRRKYHDDVSIIVISLEGRIWRSCV 716
           PAQHLVELLLFRAAKRAGMDFHELLEIPQGDRRKYHDDVSIIVISLEGRIWRSCV
Sbjct: 661 PAQHLVELLLFRAAKRAGMDFHELLEIPQGDRRKYHDDVSIIVISLEGRIWRSCV 715

BLAST of Cucsa.172480 vs. NCBI nr
Match: gi|659128285|ref|XP_008464135.1| (PREDICTED: probable protein phosphatase 2C 4 [Cucumis melo])

HSP 1 Score: 1416.0 bits (3664), Expect = 0.0e+00
Identity = 700/715 (97.90%), Postives = 707/715 (98.88%), Query Frame = 1

Query: 1   MGNGIGKLGVCFTGINDGRRRPDISVLLTNPLDEGLGHSFCYVRPDPLRLSLSKVHSEET 60
           MGNGIGKLGVCFTGINDGRRRPDISVLLTNPLDEGLGHSFCYVRPDPLRLSLSKVHSEET
Sbjct: 1   MGNGIGKLGVCFTGINDGRRRPDISVLLTNPLDEGLGHSFCYVRPDPLRLSLSKVHSEET 60

Query: 61  TTFRSISGASVSANTSTPLSTALIDLYSYNSIDRAAAFESSTSFASIPLQPIPRNMINSG 120
           TTFRSISGASVSANTSTPLSTAL+DLYSYNSIDRAAAFESSTSFASIPLQPIPRNMINSG
Sbjct: 61  TTFRSISGASVSANTSTPLSTALVDLYSYNSIDRAAAFESSTSFASIPLQPIPRNMINSG 120

Query: 121 PISGNFSGIPCSGPLERGFLSGPIERGFMSGPLDRGIFSGPLDNNSTSNEFQRSFSHGAG 180
           PISGNFSGIPCSGPLERGFLSGPIERGFMSGPLDRGIFSGPLDNNSTSNEFQRSFSHGAG
Sbjct: 121 PISGNFSGIPCSGPLERGFLSGPIERGFMSGPLDRGIFSGPLDNNSTSNEFQRSFSHGAG 180

Query: 181 FNSRSRSRKSRLIRILRTAISKSLHRGQNSIVAPIKPVKEPDWIVLPEKHNENLTVSSLN 240
           FNSRSRSRKSRLIRILRTAISKS+HRGQNSIVAPIKPVKEPDWIVLPEKHNENLTVSSLN
Sbjct: 181 FNSRSRSRKSRLIRILRTAISKSIHRGQNSIVAPIKPVKEPDWIVLPEKHNENLTVSSLN 240

Query: 241 FSSEGSLDDDDSMESQNLQWAQGKAGEDRVHVVVSEEHGWVFVGIYDGFNGPDAPDYLLS 300
           FSSEGSL+DDDSMESQNLQWAQGKAGEDRVHVVVSEEHGWVFVGIYDGFNGPDAPDYLLS
Sbjct: 241 FSSEGSLEDDDSMESQNLQWAQGKAGEDRVHVVVSEEHGWVFVGIYDGFNGPDAPDYLLS 300

Query: 301 NLYSSVHKELKGLLWDEKLDSAIVAAPATSPVQSENPLSETRDLGRNRVSDACSQCVEQE 360
           NLYS VHKELKGLLWDEKLDS  VAAP+ SPVQSENP SETRDL RNRVSDACSQCVEQE
Sbjct: 301 NLYSYVHKELKGLLWDEKLDSTAVAAPSASPVQSENPCSETRDLERNRVSDACSQCVEQE 360

Query: 361 NHPCVSGDGNSGLNSRKRKGKNLRGKFRGAAEKWEDNQRRWKCEWDRERLELDRRLKEQL 420
           NHPCVSGDGNSGL SRKRKGKNLRGKFRGAAEKWEDNQRRWKCEWDRERLELDRRLKEQL
Sbjct: 361 NHPCVSGDGNSGLKSRKRKGKNLRGKFRGAAEKWEDNQRRWKCEWDRERLELDRRLKEQL 420

Query: 421 NRSGSNGSRLINHSDVLKALSQALKKTEEAYLDIADKMLDENPELALMGSCVLVMLMKGD 480
           NRSGSNGSR INHSDVLKALSQALKKTEE+YLDIADKMLDENPELALMGSCVLVMLMKGD
Sbjct: 421 NRSGSNGSRSINHSDVLKALSQALKKTEESYLDIADKMLDENPELALMGSCVLVMLMKGD 480

Query: 481 DVYVMNVGDSRAVLAQKAEPDYWLGKIRQDLERINEEPVHDIEALDGDGAFTSANLTAFQ 540
           DVYVMNVGDSRAVLAQKAEPDYWLGKIRQDLERI+EEPVHDIEAL+GDGAFTSANLTAFQ
Sbjct: 481 DVYVMNVGDSRAVLAQKAEPDYWLGKIRQDLERISEEPVHDIEALEGDGAFTSANLTAFQ 540

Query: 541 LSMDHSTSVDEEVQRIKNEHPDDAGAVMNDRVKGSLKVTRAFGAGFLKQPKWNNALLEMF 600
           LSMDHSTSVDEEVQRIKNEHPDDAGAVMNDRVKGSLKVTRAFGAGFLKQPKWNNALLEMF
Sbjct: 541 LSMDHSTSVDEEVQRIKNEHPDDAGAVMNDRVKGSLKVTRAFGAGFLKQPKWNNALLEMF 600

Query: 601 RIDYVGRSPYINCLPSLYHHRLGPKDRFLILSSDGLYQYFTNEEAVSEVELFIALQPEGD 660
           RIDYVGRSPYINCLPSLYHHRLGPKDRFLILSSDGLYQYFTNEEAVSEVELFIALQPEGD
Sbjct: 601 RIDYVGRSPYINCLPSLYHHRLGPKDRFLILSSDGLYQYFTNEEAVSEVELFIALQPEGD 660

Query: 661 PAQHLVELLLFRAAKRAGMDFHELLEIPQGDRRKYHDDVSIIVISLEGRIWRSCV 716
           PAQHLVELLLFRAAKRAGMDFHELLEIPQGDRRKYHDDVSIIVISLEGRIWRSCV
Sbjct: 661 PAQHLVELLLFRAAKRAGMDFHELLEIPQGDRRKYHDDVSIIVISLEGRIWRSCV 715

BLAST of Cucsa.172480 vs. NCBI nr
Match: gi|1009115223|ref|XP_015874113.1| (PREDICTED: probable protein phosphatase 2C 4 [Ziziphus jujuba])

HSP 1 Score: 1187.6 bits (3071), Expect = 0.0e+00
Identity = 600/728 (82.42%), Postives = 647/728 (88.87%), Query Frame = 1

Query: 1   MGNGIGKLGVCFTGINDGRRR--PDISVLLTNPLDEGLGHSFCYVRPDPLRLSLSKVHSE 60
           MGNG GKL VCFTG  + RRR   DIS+L++ PLDEGLGHSFCYVRPDP RLS SKVHSE
Sbjct: 1   MGNGFGKLSVCFTGNGEDRRRHHQDISLLVSEPLDEGLGHSFCYVRPDPYRLSSSKVHSE 60

Query: 61  ETTTFRSISGASVSANTSTPLSTALIDLYSYNSIDRAAAFESSTSFASIPLQPIPRNMIN 120
           ETTTFR+ISGASVSANTSTPLSTA  DLYSYNSIDRAAAFESSTSFASIPLQPIPRN +N
Sbjct: 61  ETTTFRTISGASVSANTSTPLSTAF-DLYSYNSIDRAAAFESSTSFASIPLQPIPRNFMN 120

Query: 121 SGPISGNFSGIPCSGPLERGFLSGPIERGFMSGPLDRGIFSGPLDNNSTSNEFQRSFSHG 180
           SGP SGN +GIPCSGPLERGFLSGPIERGFMSGPLDRG++SGP++ +S S++FQRSFSHG
Sbjct: 121 SGPFSGNLTGIPCSGPLERGFLSGPIERGFMSGPLDRGLYSGPMEKDS-SDQFQRSFSHG 180

Query: 181 AGFNSRSRSRKSRLIRILRTAISKSLHRGQNSIVAPIKPV---KEPDWIVLPEKHNENLT 240
           A F  R RSRK +LIR+ R A+SK++ RGQNSIVAPIK V   KEPDWIV  EKHNENLT
Sbjct: 181 A-FAFRPRSRKEKLIRVFRRALSKTISRGQNSIVAPIKSVVSVKEPDWIVGSEKHNENLT 240

Query: 241 VSSLNFSSEGSLDDDDSMESQNLQWAQGKAGEDRVHVVVSEEHGWVFVGIYDGFNGPDAP 300
           VSSLN SS+GSL+DDDS+ESQNLQWAQGKAGEDRVHVVVSEEHGWVFVGIYDGFNGPDAP
Sbjct: 241 VSSLNLSSDGSLEDDDSLESQNLQWAQGKAGEDRVHVVVSEEHGWVFVGIYDGFNGPDAP 300

Query: 301 DYLLSNLYSSVHKELKGLLWDEKLDSAIVAAPATSPVQSE--NPLSETR------DLGRN 360
           DYLLSNLYS+VHKELKGLLWDEKLD+A  AAPA SPV SE  NP  E        D+ RN
Sbjct: 301 DYLLSNLYSAVHKELKGLLWDEKLDTA-TAAPAASPVHSESSNPDMEDSLRNSDSDMVRN 360

Query: 361 RVSDACSQCVEQENHPCVSGDGNSGLNSRKRKGKNLRGKFRGAAEKWEDNQRRWKCEWDR 420
           RV + CSQCVEQENHPC SGD     N R++KG+N + K+RGAA+KWEDNQRRW+CEWDR
Sbjct: 361 RVVEVCSQCVEQENHPCASGDEYFDSNFRRKKGRNTKSKYRGAAKKWEDNQRRWRCEWDR 420

Query: 421 ERLELDRRLKEQLNRSGSNGSRLINHSDVLKALSQALKKTEEAYLDIADKMLDENPELAL 480
           ER+ELDRRLKEQLNRSGS+GSR INHSDVLKALSQAL+KTEEAYLDIADKM+ ENPELAL
Sbjct: 421 ERVELDRRLKEQLNRSGSDGSRAINHSDVLKALSQALRKTEEAYLDIADKMVMENPELAL 480

Query: 481 MGSCVLVMLMKGDDVYVMNVGDSRAVLAQKAEPDYWLGKIRQDLERINEEPVHDIEALDG 540
           MGSCVLVMLMKG+DVYVMNVGDSRAVLAQKAEPDYWLGKIRQDLERINEE +HD+E  DG
Sbjct: 481 MGSCVLVMLMKGEDVYVMNVGDSRAVLAQKAEPDYWLGKIRQDLERINEETLHDLEGSDG 540

Query: 541 DGAFTSANLTAFQLSMDHSTSVDEEVQRIKNEHPDDAGAVMNDRVKGSLKVTRAFGAGFL 600
           D + T   LTA QLS DHSTSVDEEVQRIKNEHPDD+ AV+NDRVKGSLKVTRAFGAGFL
Sbjct: 541 DRSSTIPTLTALQLSKDHSTSVDEEVQRIKNEHPDDSCAVVNDRVKGSLKVTRAFGAGFL 600

Query: 601 KQPKWNNALLEMFRIDYVGRSPYINCLPSLYHHRLGPKDRFLILSSDGLYQYFTNEEAVS 660
           KQPKWNNALLE+FRIDY+G SPYI C P LYHHRLGPKDRFLILSSDGLYQYFTNEEAVS
Sbjct: 601 KQPKWNNALLEVFRIDYIGSSPYITCFPFLYHHRLGPKDRFLILSSDGLYQYFTNEEAVS 660

Query: 661 EVELFIALQPEGDPAQHLVELLLFRAAKRAGMDFHELLEIPQGDRRKYHDDVSIIVISLE 716
           EVELFI LQPEGDPAQHLVE +LFRAAK AGMDFHELLEIPQGDRR+YHDDVSIIVISLE
Sbjct: 661 EVELFITLQPEGDPAQHLVEEVLFRAAKEAGMDFHELLEIPQGDRRRYHDDVSIIVISLE 720

BLAST of Cucsa.172480 vs. NCBI nr
Match: gi|731392219|ref|XP_010651025.1| (PREDICTED: probable protein phosphatase 2C 23 [Vitis vinifera])

HSP 1 Score: 1152.5 bits (2980), Expect = 0.0e+00
Identity = 577/726 (79.48%), Postives = 639/726 (88.02%), Query Frame = 1

Query: 1   MGNGIGKLGVCFTGINDGRRRPDISVLLTNPLDEGLGHSFCYVRPDPLRLSLSKVHSEET 60
           MGNG  KL +CFTG    RRR DISVL+++PLDEGLGHSFCY+RPD  RLS SKVHSEET
Sbjct: 1   MGNGFAKLSICFTGEGGARRRQDISVLISDPLDEGLGHSFCYIRPDQSRLSSSKVHSEET 60

Query: 61  TTFRSISGASVSANTSTPLSTALIDLYSYNSIDRAAAFESSTSFASIPLQPIPRNMINSG 120
           TTFRSISGASVSANTSTPLSTA +DLYSYNSIDRA+AFESSTSF SIPLQPIPRN +NSG
Sbjct: 61  TTFRSISGASVSANTSTPLSTAFVDLYSYNSIDRASAFESSTSFTSIPLQPIPRNWMNSG 120

Query: 121 PISGNFSGIPCSGPLERGFLSGPIERGFMSGPLDRGIFSGPLDNNSTSNEFQRSFSHGAG 180
           PI G++ GIP SGPLERGFLSGPIERGFMSGP+DRG+FSGPL+ +ST ++FQRS+SHG G
Sbjct: 121 PIPGSYGGIPGSGPLERGFLSGPIERGFMSGPIDRGLFSGPLEKSST-DQFQRSYSHG-G 180

Query: 181 FNSRSRSRKSRLIRILRTAISKSLHRGQNSIVAPIK---PVKEPDWIVLPEKHNENLTVS 240
           F  R RSRK  LIR+L+ AISK++ RGQNSIVAPIK    VKEPDW+V  EKHNENLTVS
Sbjct: 181 FAFRPRSRKGSLIRVLQRAISKTISRGQNSIVAPIKGVVSVKEPDWLVGSEKHNENLTVS 240

Query: 241 SLNFSSEGSLDDDDSMESQNLQWAQGKAGEDRVHVVVSEEHGWVFVGIYDGFNGPDAPDY 300
           S+N SS+GSL+DDDS+ESQNLQWAQGKAGEDRVHVVVSEEHGWVFVGIYDGFNGPDAPDY
Sbjct: 241 SVNLSSDGSLEDDDSLESQNLQWAQGKAGEDRVHVVVSEEHGWVFVGIYDGFNGPDAPDY 300

Query: 301 LLSNLYSSVHKELKGLLWDEKLDSAIVAAPATSPVQSENPLSETRD--------LGRNRV 360
           LLSNLYS+VHKELKGLLWD+K +S  VAAPA+SPV SE   SE  D        L RNR+
Sbjct: 301 LLSNLYSAVHKELKGLLWDDKHESNPVAAPASSPVPSEASNSELEDSHLGSDVDLARNRM 360

Query: 361 SDACSQCVEQENHPCVSGDGNSGLNSRKRKGKNLRGKFRGAAEKWEDNQRRWKCEWDRER 420
            D CS C  QE +P  SGD     NS+++KGKN + K++GAA+KWE+NQRRWKCEWDRER
Sbjct: 361 VDGCSHCSYQEYYPSGSGDVKFDSNSKRKKGKNSKNKYKGAAKKWEENQRRWKCEWDRER 420

Query: 421 LELDRRLKEQLNRSGSNGSRLINHSDVLKALSQALKKTEEAYLDIADKMLDENPELALMG 480
           LELDRRLKEQLN S ++GSR INHSDVLKALSQAL+KTEE+YL+IADKM+ ENPELALMG
Sbjct: 421 LELDRRLKEQLNGSNTDGSRSINHSDVLKALSQALRKTEESYLEIADKMVMENPELALMG 480

Query: 481 SCVLVMLMKGDDVYVMNVGDSRAVLAQKAEPDYWLGKIRQDLERINEEPVHDIEALDGDG 540
           SCVLVMLMKG+DVYVMNVGDSRAVLAQKAE D WLGKIRQDLERINEE +HD+EA+D D 
Sbjct: 481 SCVLVMLMKGEDVYVMNVGDSRAVLAQKAEADVWLGKIRQDLERINEETLHDLEAMDNDN 540

Query: 541 AFTSANLTAFQLSMDHSTSVDEEVQRIKNEHPDDAGAVMNDRVKGSLKVTRAFGAGFLKQ 600
           +     L+AFQL++DHSTSV+EEV+RIKNEHPDDA AVMNDRVKGSLKVTRAFGAGFLKQ
Sbjct: 541 SNMIPTLSAFQLTVDHSTSVEEEVRRIKNEHPDDACAVMNDRVKGSLKVTRAFGAGFLKQ 600

Query: 601 PKWNNALLEMFRIDYVGRSPYINCLPSLYHHRLGPKDRFLILSSDGLYQYFTNEEAVSEV 660
           PKWNNALLEMFRIDYVG SPYI+CLPSLYHHRLGP+DRFLILSSDGLYQY TNEEAVSEV
Sbjct: 601 PKWNNALLEMFRIDYVGTSPYISCLPSLYHHRLGPEDRFLILSSDGLYQYLTNEEAVSEV 660

Query: 661 ELFIALQPEGDPAQHLVELLLFRAAKRAGMDFHELLEIPQGDRRKYHDDVSIIVISLEGR 716
           ELFIAL P+GDPAQHLVE +LFRAAK+AGMDFHELLEIPQGDRR+YHDDVSIIVISLEG 
Sbjct: 661 ELFIALSPDGDPAQHLVEEVLFRAAKKAGMDFHELLEIPQGDRRRYHDDVSIIVISLEGM 720

BLAST of Cucsa.172480 vs. NCBI nr
Match: gi|703137526|ref|XP_010106468.1| (putative protein phosphatase 2C 4 [Morus notabilis])

HSP 1 Score: 1122.5 bits (2902), Expect = 0.0e+00
Identity = 588/744 (79.03%), Postives = 636/744 (85.48%), Query Frame = 1

Query: 1   MGNGIGKLGVCFTGINDGRRRPDISVLLTNPLDEGLGHSFCYVRPDPLRLSLSKVHSEET 60
           MGNG GKL VCFTG  D RRR DI + L +PLDEGLGHSFCYVRPDP RLS SKVHSEET
Sbjct: 1   MGNGFGKLTVCFTGGGDARRRHDIPLYLPDPLDEGLGHSFCYVRPDPTRLSSSKVHSEET 60

Query: 61  TTFRSISGASVSANTSTPLSTALIDLYSYNSIDRAAAFESSTSFASIPLQPIPRNMINSG 120
           TTF++ISGASVSANTSTPLSTALIDLYSYNSIDRAAAFESSTSFASIPLQPIPRN I SG
Sbjct: 61  TTFKTISGASVSANTSTPLSTALIDLYSYNSIDRAAAFESSTSFASIPLQPIPRNFIGSG 120

Query: 121 PISGNFSGIPCSGPLERGFLSGPIERGFMSGPLDRGIFSGPLDNNS--TSNEFQRSFSHG 180
           PIS  F GIP SGPLERGF+SGPIERGFMSGPLDRG+FSGP+D +   T+N+FQRSFS G
Sbjct: 121 PISAPF-GIPSSGPLERGFMSGPIERGFMSGPLDRGLFSGPMDKSGPDTNNQFQRSFSQG 180

Query: 181 AGFNSRSRSRKSRLIRILRTAISKSLHRGQNSIVAPIK-----PVKEPDWIVLPEKHN-- 240
            GF  R RS K +LIR LR AISK+++RGQNSIVAPIK      VKEP+W    EKHN  
Sbjct: 181 -GFAFRPRSAKGKLIRALRRAISKTINRGQNSIVAPIKGLSLSSVKEPEWNFGLEKHNHH 240

Query: 241 --ENLTVSSLNFSSEGSLDDDDSMESQNLQWAQGKAGEDRVHVVVSEEHGWVFVGIYDGF 300
             ENLTVSSLN SSEGSL+DDDS ESQNLQWAQGKAGEDRVHVVVSEEHGWVFVGIYDGF
Sbjct: 241 HNENLTVSSLNLSSEGSLEDDDSFESQNLQWAQGKAGEDRVHVVVSEEHGWVFVGIYDGF 300

Query: 301 NGPDAPDYLLSNLYSSVHKELKGLLWDEKLD--SAIVAAPATSPVQSENPLSETRD---- 360
           NGPDAPDYLLSNLYS+VHKELKGLLWDEK +  SA  AA AT P   +NP SE  D    
Sbjct: 301 NGPDAPDYLLSNLYSAVHKELKGLLWDEKFEPASAAAAAAATFP---DNPNSEAADQDSL 360

Query: 361 -------LGRNRVSD-ACSQCVEQENH-PCVSGD-GNSGLNSRKRKGKN-LRGKFRG-AA 420
                  LG N+V D AC QCVEQEN+ P  SGD GN   NSR++K ++  + K+RG A 
Sbjct: 361 PSVADSDLGGNQVGDDACFQCVEQENNLPWASGDDGNCVSNSRRKKCQSGSKSKYRGDAT 420

Query: 421 EKWEDNQRRWKCEWDRERLELDRRLKEQLNRSGSNGSRLINHSDVLKALSQALKKTEEAY 480
            KWEDNQ+RW+CEWDRERLELDRRLKEQLNRSGS+G   INHSDVLKALSQAL+KTEEAY
Sbjct: 421 NKWEDNQKRWRCEWDRERLELDRRLKEQLNRSGSDGGGAINHSDVLKALSQALRKTEEAY 480

Query: 481 LDIADKMLDENPELALMGSCVLVMLMKGDDVYVMNVGDSRAVLAQKAEPDYWLGKIRQDL 540
           LDIADKML ENPELALMGSCVLVMLMKG+DVYVMNVGDSRAVLAQK+EPDYWLGKIRQDL
Sbjct: 481 LDIADKMLVENPELALMGSCVLVMLMKGEDVYVMNVGDSRAVLAQKSEPDYWLGKIRQDL 540

Query: 541 ERINEEPVHDIEALDGDGAFTSANLTAFQLSMDHSTSVDEEVQRIKNEHPDDAGAVMNDR 600
           ERINEE +HD+E+ DG+   T A+LTA QL+ DHSTSV+EEVQRI+ EHPDD+ AV+NDR
Sbjct: 541 ERINEETLHDLESPDGERPNTIASLTALQLTKDHSTSVEEEVQRIREEHPDDSCAVINDR 600

Query: 601 VKGSLKVTRAFGAGFLKQPKWNNALLEMFRIDYVGRSPYINCLPSLYHHRLGPKDRFLIL 660
           VKGSLKVTRAFGAGFLKQPKWNNALLEMFRIDY+G SPYI C P L+HHRLGPKDRFLIL
Sbjct: 601 VKGSLKVTRAFGAGFLKQPKWNNALLEMFRIDYIGTSPYITCFPFLHHHRLGPKDRFLIL 660

Query: 661 SSDGLYQYFTNEEAVSEVELFIALQPEGDPAQHLVELLLFRAAKRAGMDFHELLEIPQGD 716
           SSDGLYQYFTNEEAVSEVELFI LQPEGDPAQHLVE +LFRAAK+AGMDFHELLEIPQGD
Sbjct: 661 SSDGLYQYFTNEEAVSEVELFITLQPEGDPAQHLVEEVLFRAAKKAGMDFHELLEIPQGD 720