Cucsa.169870 (gene) Cucumber (Gy14) v1

Overview
NameCucsa.169870
Typegene
OrganismCucumis sativus (Cucumber (Gy14) v1)
DescriptionGag-pol polyprotein, putative
Locationscaffold01174 : 70796 .. 71178 (-)
Genome Viewer
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGACAACATCTATGAGATCTCAACATCGGATGAAGGAGATCAACTATCTTGTGTAGTTCAAAGAATTCTCTTCACTCCTACTGCTGAACAAATACTTCAAAGGAATTCCCTTTTTCGAACAAGATGCACCATTAACGGTAAAGTGTGTCAAATTATCATTGATAGTGGTAGTAGTGAAAATTTAGTGTCTAAAAAACTAGTTTCTGCCCTAAATTTAAAAATTGACCCACACCCGAATTCCTATAAGGTTAGTTCGATAAAAAAGGGAGGAGAAGCTACTGTCAGTGAGGTTTGTACTATTTCTTTATCAATAGGACAGCACTATAAAGGCCAAATTATATGTGATGTTCTTGATATGGATGTTTGCCACATCCTTCTTTGA

mRNA sequence

atgacaacatctatgagatctcaacatcggatgaaggagatcaactatcttgtgtagttcaaagaattctcttcactcctactgctgaacaaatacttcaaaggaattccctttttcgaacaagatgcaccattaacggtaaagtgtgtcaaattatcattgatagtggtagtagtgaaaatttagtgtctaaaaaactagtttctgccctaaatttaaaaattgacccacacccgaattcctataaggttagttcgataaaaaagggaggagaagctactgtcagtgaggtttgtactatttctttatcaataggacagcactataaaggccaaattatatgtgatgttcttgatatggatgtttgccacatccttctttga

Coding sequence (CDS)

ATGACAACATCTATGAGATCTCAACATCGGATGAAGGAGATCAACTATCTTGTGTAGTTCAAAGAATTCTCTTCACTCCTACTGCTGAACAAATACTTCAAAGGAATTCCCTTTTTCGAACAAGATGCACCATTAACGGTAAAGTGTGTCAAATTATCATTGATAGTGGTAGTAGTGAAAATTTAGTGTCTAAAAAACTAGTTTCTGCCCTAAATTTAAAAATTGACCCACACCCGAATTCCTATAAGGTTAGTTCGATAAAAAAGGGAGGAGAAGCTACTGTCAGTGAGGTTTGTACTATTTCTTTATCAATAGGACAGCACTATAAAGGCCAAATTATATGTGATGTTCTTGATATGGATGTTTGCCACATCCTTCTTTGA

Protein sequence

DNIYEISTSDEGDQLSCVVQRILFTPTAEQILQRNSLFRTRCTINGKVCQIIIDSGSSENLVSKKLVSALNLKIDPHPNSYKVSSIKKGGEATVSEVCTISLSIGQHYKGQIICDVLDMDVCHILL*
Homology
[Close]
BLAST of Cucsa.169870 vs. TrEMBL
Match: A5BLC8_VITVI (Putative uncharacterized protein OS=Vitis vinifera GN=VITISV_019345 PE=4 SV=1)

HSP 1 Score: 147.5 bits (371), Expect = 1.1e-32
Identity = 70/122 (57.38%), Postives = 90/122 (73.77%), Query Frame = 1 

Query: 5   EISTSDEGDQLSCVVQRILFTPTAEQILQRNSLFRTRCTINGKVCQIIIDSGSSENLVSK 64
           E +  D G++++C+VQR+L T       QR+ +FRT+CTI  KVC +IIDSGSSEN VSK
Sbjct: 715 EFAEGDVGEEVTCIVQRLLLTLKKSDDSQRHKIFRTQCTIRNKVCNVIIDSGSSENFVSK 774

Query: 65  KLVSALNLKIDPHPNSYKVSSIKKGGEATVSEVCTISLSIGQHYKGQIICDVLDMDVCHI 124
            LV ALNLK   HP+SYK++ I KG +  V EVC I LSIG++YK +I+CDVLDMD C+I
Sbjct: 775 ALVKALNLKTKEHPSSYKIAWINKGMKVQVLEVCKIPLSIGKYYKDEIVCDVLDMDACYI 834

Query: 125 LL 127
           LL
Sbjct: 835 LL 836

[Close]
BLAST of Cucsa.169870 vs. TrEMBL
Match: M5WCC7_PRUPE (Uncharacterized protein OS=Prunus persica GN=PRUPE_ppa017790mg PE=4 SV=1)

HSP 1 Score: 126.7 bits (317), Expect = 2.0e-26
Identity = 57/122 (46.72%), Postives = 84/122 (68.85%), Query Frame = 1 

Query: 5   EISTSDEGDQLSCVVQRILFTPTAEQILQRNSLFRTRCTINGKVCQIIIDSGSSENLVSK 64
           E +  +  ++++ V+QR+L  P  E   QR+S+FR+ C+I  KVC +I+D+GS EN VSK
Sbjct: 394 EFAVEEGMEKITLVLQRVLLAPREEG--QRHSIFRSLCSIKNKVCDVIVDNGSCENFVSK 453

Query: 65  KLVSALNLKIDPHPNSYKVSSIKKGGEATVSEVCTISLSIGQHYKGQIICDVLDMDVCHI 124
           KLV  L L  +PH + Y +  +KKG    V+E C + LSIG+HY+ +++CDV+DMD CHI
Sbjct: 454 KLVEYLQLSTEPHVSPYSLGWVKKGPSVRVAETCRVPLSIGKHYRDEVLCDVIDMDACHI 513

Query: 125 LL 127
           LL
Sbjct: 514 LL 513

[Close]
BLAST of Cucsa.169870 vs. TrEMBL
Match: M5W531_PRUPE (Uncharacterized protein OS=Prunus persica GN=PRUPE_ppa026856mg PE=4 SV=1)

HSP 1 Score: 124.4 bits (311), Expect = 9.9e-26
Identity = 56/122 (45.90%), Postives = 83/122 (68.03%), Query Frame = 1 

Query: 5   EISTSDEGDQLSCVVQRILFTPTAEQILQRNSLFRTRCTINGKVCQIIIDSGSSENLVSK 64
           E +  +  ++++ V+QR+L  P  E   QR+++FR+ C+I  KVC +I+D+GS EN VSK
Sbjct: 405 EFAVEEGIEKITLVLQRVLLAPKEEG--QRHNIFRSLCSIKNKVCDVIVDNGSCENFVSK 464

Query: 65  KLVSALNLKIDPHPNSYKVSSIKKGGEATVSEVCTISLSIGQHYKGQIICDVLDMDVCHI 124
           KLV  L L  +PH + Y +  +KKG    V+E C + LSIG+HY+  ++CDV+DMD CHI
Sbjct: 465 KLVEYLQLSTEPHVSPYSLGWVKKGPSVRVAETCRVPLSIGKHYRDDVLCDVIDMDACHI 524

Query: 125 LL 127
           LL
Sbjct: 525 LL 524

[Close]
BLAST of Cucsa.169870 vs. TrEMBL
Match: Q9ZQ09_ARATH (Putative Ty3-gypsy-like retroelement pol polyprotein OS=Arabidopsis thaliana GN=At2g06170 PE=4 SV=1)

HSP 1 Score: 121.7 bits (304), Expect = 6.4e-25
Identity = 59/122 (48.36%), Postives = 82/122 (67.21%), Query Frame = 1 

Query: 5   EISTSDEGDQLSCVVQRILFTPTAEQILQRNSLFRTRCTINGKVCQIIIDSGSSENLVSK 64
           E +  +  + ++ V+QRIL +   E   QR +LFRTRC+IN KVC +I+D GSSENLVS+
Sbjct: 194 EFAEEESNEMINLVLQRILLSSKEEG--QRRNLFRTRCSINDKVCNLIVDIGSSENLVSQ 253

Query: 65  KLVSALNLKIDPHPNSYKVSSIKKGGEATVSEVCTISLSIGQHYKGQIICDVLDMDVCHI 124
           KLV  L L    H   Y +  + KG +  VS  C + +SIG+HYK +++CDVL+MDVCHI
Sbjct: 254 KLVEYLKLPTTLHQKPYSLGWVSKGSQFCVSLSCRVPISIGKHYKEEVLCDVLNMDVCHI 313

Query: 125 LL 127
           +L
Sbjct: 314 IL 313

[Close]
BLAST of Cucsa.169870 vs. TrEMBL
Match: A0A0Q3MM81_BRADI (Uncharacterized protein OS=Brachypodium distachyon GN=BRADI_2g19456 PE=4 SV=1)

HSP 1 Score: 117.1 bits (292), Expect = 1.6e-23
Identity = 50/117 (42.74%), Postives = 81/117 (69.23%), Query Frame = 1 

Query: 10  DEGDQLSCVVQRILFTPTAEQILQRNSLFRTRCTINGKVCQIIIDSGSSENLVSKKLVSA 69
           +EG++++CVV+R++ +       QR  +F ++CT+NGKVC+++IDS S ENL+S+ LV+ 
Sbjct: 212 EEGEEVACVVRRLVCSTPQADNTQRKKIFESKCTVNGKVCKLVIDSCSCENLISQNLVNY 271

Query: 70  LNLKIDPHPNSYKVSSIKKGGEATVSEVCTISLSIGQHYKGQIICDVLDMDVCHILL 127
           L L+   H N Y +  IKKG    V++ C + LS+G++Y   ++CDV+DMD  H+LL
Sbjct: 272 LKLETHDHTNPYTIGWIKKGMNMRVTKQCNLPLSLGKYYHSNVLCDVVDMDASHVLL 328

[Close]
BLAST of Cucsa.169870 vs. NCBI nr
Match: gi|659121770|ref|XP_008460803.1| (PREDICTED: uncharacterized protein LOC103499566 [Cucumis melo])

HSP 1 Score: 206.1 bits (523), Expect = 3.7e-50
Identity = 104/126 (82.54%), Postives = 111/126 (88.10%), Query Frame = 1 

Query: 1   DNIYEISTSDEGDQLSCVVQRILFTPTAEQILQRNSLFRTRCTINGKVCQIIIDSGSSEN 60
           DNIYEIST DEGDQLSCV+QRILFTPT ++I QRNSLF+TRCTI  KVCQ+II SGS +N
Sbjct: 76  DNIYEISTPDEGDQLSCVIQRILFTPTTDRIPQRNSLFQTRCTIQDKVCQVIIYSGS-QN 135

Query: 61  LVSKKLVSALNLKIDPHPNSYKVSSIKKGGEATVSEVCTISLSIGQHYKGQIICDVLDMD 120
           LVSKKLVS LNLKIDPH N YKVS I KGGEA V+EVCTISLSIGQHYK QIICDVLDMD
Sbjct: 136 LVSKKLVSTLNLKIDPHLNPYKVSWINKGGEANVNEVCTISLSIGQHYKDQIICDVLDMD 195

Query: 121 VCHILL 127
            CHILL
Sbjct: 196 ACHILL 200

[Close]
BLAST of Cucsa.169870 vs. NCBI nr
Match: gi|659102468|ref|XP_008452148.1| (PREDICTED: uncharacterized protein LOC103493250 [Cucumis melo])

HSP 1 Score: 171.8 bits (434), Expect = 7.8e-40
Identity = 77/122 (63.11%), Postives = 101/122 (82.79%), Query Frame = 1 

Query: 5   EISTSDEGDQLSCVVQRILFTPTAEQILQRNSLFRTRCTINGKVCQIIIDSGSSENLVSK 64
           E+  +D GD++SC+VQR+L T   E+  QR+SLF+TRCTINGKVC +IIDSGSSEN V++
Sbjct: 152 ELIEADNGDRISCIVQRVLITLKEERNPQRHSLFKTRCTINGKVCDVIIDSGSSENFVAR 211

Query: 65  KLVSALNLKIDPHPNSYKVSSIKKGGEATVSEVCTISLSIGQHYKGQIICDVLDMDVCHI 124
           KLV++LNLKIDPHP+ YK+  +KK GE  ++E+CTI LSIG  YK QI+CDV++MDVCH+
Sbjct: 212 KLVTSLNLKIDPHPDPYKIGWVKKEGETLINEICTIPLSIGNSYKDQIVCDVIEMDVCHL 271

Query: 125 LL 127
           LL
Sbjct: 272 LL 273

[Close]
BLAST of Cucsa.169870 vs. NCBI nr
Match: gi|778664952|ref|XP_011648447.1| (PREDICTED: uncharacterized protein LOC105434464 [Cucumis sativus])

HSP 1 Score: 168.7 bits (426), Expect = 6.6e-39
Identity = 78/132 (59.09%), Postives = 103/132 (78.03%), Query Frame = 1 

Query: 5   EISTSDEGDQLSCVVQRILFTPTAEQILQRNSLFRTRCTINGKVC----------QIIID 64
           E+  +D+G+++SCV+QR+L TP  E+ LQR+ LF+TRCTING+VC           +IID
Sbjct: 196 ELIEADDGERVSCVIQRVLITPKEEKKLQRHCLFKTRCTINGRVCDVIIDNDSSSDVIID 255

Query: 65  SGSSENLVSKKLVSALNLKIDPHPNSYKVSSIKKGGEATVSEVCTISLSIGQHYKGQIIC 124
           SGSSEN V+KKLV+ LNLK + HPN YK+  ++KGGEATVSE+CT+ LSIG  YK QI+C
Sbjct: 256 SGSSENFVAKKLVTVLNLKAEAHPNPYKIGWVRKGGEATVSEICTVPLSIGNAYKDQIVC 315

Query: 125 DVLDMDVCHILL 127
           DV++MDVCH+LL
Sbjct: 316 DVIEMDVCHLLL 327

[Close]
BLAST of Cucsa.169870 vs. NCBI nr
Match: gi|147812164|emb|CAN70290.1| (hypothetical protein VITISV_019345 [Vitis vinifera])

HSP 1 Score: 147.5 bits (371), Expect = 1.6e-32
Identity = 70/122 (57.38%), Postives = 90/122 (73.77%), Query Frame = 1 

Query: 5   EISTSDEGDQLSCVVQRILFTPTAEQILQRNSLFRTRCTINGKVCQIIIDSGSSENLVSK 64
           E +  D G++++C+VQR+L T       QR+ +FRT+CTI  KVC +IIDSGSSEN VSK
Sbjct: 715 EFAEGDVGEEVTCIVQRLLLTLKKSDDSQRHKIFRTQCTIRNKVCNVIIDSGSSENFVSK 774

Query: 65  KLVSALNLKIDPHPNSYKVSSIKKGGEATVSEVCTISLSIGQHYKGQIICDVLDMDVCHI 124
            LV ALNLK   HP+SYK++ I KG +  V EVC I LSIG++YK +I+CDVLDMD C+I
Sbjct: 775 ALVKALNLKTKEHPSSYKIAWINKGMKVQVLEVCKIPLSIGKYYKDEIVCDVLDMDACYI 834

Query: 125 LL 127
           LL
Sbjct: 835 LL 836

[Close]
BLAST of Cucsa.169870 vs. NCBI nr
Match: gi|1009139320|ref|XP_015887064.1| (PREDICTED: uncharacterized protein LOC107422168 [Ziziphus jujuba])

HSP 1 Score: 146.0 bits (367), Expect = 4.6e-32
Identity = 66/122 (54.10%), Postives = 90/122 (73.77%), Query Frame = 1 

Query: 5   EISTSDEGDQLSCVVQRILFTPTAEQILQRNSLFRTRCTINGKVCQIIIDSGSSENLVSK 64
           E+   D+G+ + C++Q++LF+P      QR+S+F+T+CTIN KVC++IIDSGSSEN+VSK
Sbjct: 220 ELVDEDQGEPVICIIQKLLFSPKHPMEPQRHSIFKTKCTINKKVCEVIIDSGSSENIVSK 279

Query: 65  KLVSALNLKIDPHPNSYKVSSIKKGGEATVSEVCTISLSIGQHYKGQIICDVLDMDVCHI 124
            LV AL L    HPN YKV  IKKG E  V E+C +  SIG+HY  +++CDV++MD CHI
Sbjct: 280 SLVKALKLPTMSHPNPYKVRWIKKGIETKVIELCKVHFSIGKHYADEVVCDVVEMDACHI 339

Query: 125 LL 127
           LL
Sbjct: 340 LL 341

The following BLAST results are available for this feature:
BLAST of Cucsa.169870 vs. TrEMBL
Match NameE-valueIdentityDescription
A5BLC8_VITVI1.1e-3257.38Putative uncharacterized protein OS=Vitis vinifera GN=VITISV_019345 PE=4 SV=1[more]
M5WCC7_PRUPE2.0e-2646.72Uncharacterized protein OS=Prunus persica GN=PRUPE_ppa017790mg PE=4 SV=1[more]
M5W531_PRUPE9.9e-2645.90Uncharacterized protein OS=Prunus persica GN=PRUPE_ppa026856mg PE=4 SV=1[more]
Q9ZQ09_ARATH6.4e-2548.36Putative Ty3-gypsy-like retroelement pol polyprotein OS=Arabidopsis thaliana GN=... [more]
A0A0Q3MM81_BRADI1.6e-2342.74Uncharacterized protein OS=Brachypodium distachyon GN=BRADI_2g19456 PE=4 SV=1[more]
BLAST of Cucsa.169870 vs. NCBI nr
Match NameE-valueIdentityDescription
gi|659121770|ref|XP_008460803.1|3.7e-5082.54PREDICTED: uncharacterized protein LOC103499566 [Cucumis melo][more]
gi|659102468|ref|XP_008452148.1|7.8e-4063.11PREDICTED: uncharacterized protein LOC103493250 [Cucumis melo][more]
gi|778664952|ref|XP_011648447.1|6.6e-3959.09PREDICTED: uncharacterized protein LOC105434464 [Cucumis sativus][more]
gi|147812164|emb|CAN70290.1|1.6e-3257.38hypothetical protein VITISV_019345 [Vitis vinifera][more]
gi|1009139320|ref|XP_015887064.1|4.6e-3254.10PREDICTED: uncharacterized protein LOC107422168 [Ziziphus jujuba][more]
Annotated Terms
The following terms have been associated with this gene:
Vocabulary: INTERPRO
TermDefinition
IPR001969Aspartic_peptidase_AS
IPR021109Peptidase_aspartic_dom_sf
Vocabulary: Molecular Function
TermDefinition
GO:0004190aspartic-type endopeptidase activity
Vocabulary: Biological Process
TermDefinition
GO:0006508proteolysis
GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006508 proteolysis
cellular_component GO:0005575 cellular_component
molecular_function GO:0004190 aspartic-type endopeptidase activity
Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Cucsa.169870.1Cucsa.169870.1mRNA


InterPro
Analysis Name: InterPro Annotations of cucumber (Gy14)
Date Performed: 2017-01-17
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001969Aspartic peptidase, active sitePROSITEPS00141ASP_PROTEASEcoord: 51..62
scor
IPR021109Aspartic peptidase domainGENE3DG3DSA:2.40.70.10coord: 47..107
score: 1.
IPR021109Aspartic peptidase domainunknownSSF50630Acid proteasescoord: 33..109
score: 3.7
NoneNo IPR availablePFAMPF13650Asp_protease_2coord: 41..119
score: 1.

Synteny
The following gene(s) are orthologous to this gene:

None

The following gene(s) are paralogous to this gene:

None

The following block(s) are covering this gene:
GeneOrganismBlock
Cucsa.169870Silver-seed gourdcarcgyB0546
Cucsa.169870Cucumber (Chinese Long) v3cgycucB271
Cucsa.169870Cucurbita maxima (Rimu)cgycmaB0482
Cucsa.169870Cucurbita moschata (Rifu)cgycmoB0485
Cucsa.169870Wild cucumber (PI 183967)cgycpiB267
Cucsa.169870Cucumber (Chinese Long) v2cgycuB259
Cucsa.169870Watermelon (Charleston Gray)cgywcgB304
Cucsa.169870Watermelon (97103) v1cgywmB325
Cucsa.169870Bottle gourd (USVL1VR-Ls)cgylsiB283
Cucsa.169870Melon (DHL92) v3.6.1cgymedB295