Cucsa.169150 (gene) Cucumber (Gy14) v1 | Cucurbit Genomics Database (CuGenDB)

Overview


Name	Cucsa.169150
Type	gene
Organism	Cucumis sativus (Cucumber (Gy14) v1)
Description	Starch branching enzyme IIb
Location	scaffold01163 : 124775 .. 125319 (+)

Genome Viewer

Gene Expression

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSthree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGATGTACACTCACCATGACTTAGAGGTAGGATTTACTGGAAACAACAGTGAGAACTTCAGATTTGCTACAGAAGTAAATGTTGTTTTGCTTTTGTCGCTGGTGAATGATATGTTTCATGGGCTTTATCTGAAGATGCCAATGGTTTGCCTACCTTTTGAATTCTTATCACAGCTCTGGAATCGGATTTGATTACTGTCTACACATAGTCATTGTTGTTAAATAGATCGAGCTTCTCAAGAATAACAATGAAGATCAGGAAAAGGGTGTTATTTTCATTCGTTTTTTAACAAAAGATGGCTAGAGAAATGAGTTGCATATGCTGAAAGTCATGACTGAGCTATTGATAATGATGAAACAGTTGCATTCCTATTGCACGATTTTGGTCATCTTGTAGATTGTGATTCCAATGTTCAGAACACGGTCATAGATTAATTGATAGTTAAGCCTCAAAATGTTGGCTGTTTGTTTCTTCAAAATTCAATCTATCAAAGAAGAAGTCTCATTTCATGCCATGTTTTTATCCACTCTAGTCTCTTCTTTTA

mRNA sequence

ATGATGTACACTCACCATGACTTAGAGGTAGGATTTACTGGAAACAACAGTGAGAACTTCAGATTTGCTACAGAAGTAAATGTTGTTTTGCTTTTGTCGCTGGTGAATGATATGTTTCATGGGCTTTATCTGAAGATGCCAATGGTTTGCCTACCTTTTGAATTCTTATCACAGCTCTGGAATCGGATTTGATTACTGTCTACACATAGTCATTGTTGTTAAATAGATCGAGCTTCTCAAGAATAACAATGAAGATCAGGAAAAGGGTGTTATTTTCATTCGTTTTTTAACAAAAGATGGCTAGAGAAATGAGTTGCATATGCTGAAAGTCATGACTGAGCTATTGATAATGATGAAACAGTTGCATTCCTATTGCACGATTTTGGTCATCTTGTAGATTGTGATTCCAATGTTCAGAACACGGTCATAGATTAATTGATAGTTAAGCCTCAAAATGTTGGCTGTTTGTTTCTTCAAAATTCAATCTATCAAAGAAGAAGTCTCATTTCATGCCATGTTTTTATCCACTCTAGTCTCTTCTTTTA

Coding sequence (CDS)

ATGATGTACACTCACCATGACTTAGAGGTAGGATTTACTGGAAACAACAGTGAGAACTTCAGATTTGCTACAGAAGTAAATGTTGTTTTGCTTTTGTCGCTGGTGAATGATATGTTTCATGGGCTTTATCTGAAGATGCCAATGGTTTGCCTACCTTTTGAATTCTTATCACAGCTCTGGAATCGGATTTGA

Protein sequence

MMYTHHDLEVGFTGNNSENFRFATEVNVVLLLSLVNDMFHGLYLKMPMVCLPFEFLSQLWNRI*

Homology

[Close]

BLAST of Cucsa.169150 vs. Swiss-Prot
Match: GLGB1_ARATH (1,4-alpha-glucan-branching enzyme 2-1, chloroplastic/amyloplastic OS=Arabidopsis thaliana GN=SBE2.1 PE=2 SV=1)

HSP 1 Score: 63.2 bits (152), Expect = 1.2e-09
Identity = 31/64 (48.44%), Postives = 41/64 (64.06%), Query Frame = 1


Query: 1   MMYTHHDLEVGFTGNNSENFRFATEVNVVLLLSLVNDMFHGLYLK----------MPMVC 55
           MMYTHH L+V FTGN +E F ++T+V+ V+ L LVND+ HGLY +          MP  C
Sbjct: 491 MMYTHHGLQVEFTGNYNEYFGYSTDVDAVVYLMLVNDLIHGLYPEAIVVGEDVSGMPAFC 550

[Close]

BLAST of Cucsa.169150 vs. Swiss-Prot
Match: GLGB2_ARATH (1,4-alpha-glucan-branching enzyme 2-2, chloroplastic/amyloplastic OS=Arabidopsis thaliana GN=SBE2.2 PE=2 SV=1)

HSP 1 Score: 62.4 bits (150), Expect = 2.1e-09
Identity = 32/64 (50.00%), Postives = 38/64 (59.38%), Query Frame = 1


Query: 1   MMYTHHDLEVGFTGNNSENFRFATEVNVVLLLSLVNDMFHGLYLK----------MPMVC 55
           MMYTHH L VGFTGN +E F   T+V+ V  L LVNDM HGLY +          MP  C
Sbjct: 456 MMYTHHGLSVGFTGNYTEYFGLETDVDAVNYLMLVNDMIHGLYPEAITVGEDVSGMPTFC 515

[Close]

BLAST of Cucsa.169150 vs. Swiss-Prot
Match: GLGB_MAIZE (1,4-alpha-glucan-branching enzyme 2, chloroplastic/amyloplastic OS=Zea mays GN=SBE1 PE=1 SV=1)

HSP 1 Score: 62.0 bits (149), Expect = 2.7e-09
Identity = 28/43 (65.12%), Postives = 34/43 (79.07%), Query Frame = 1


Query: 1   MMYTHHDLEVGFTGNNSENFRFATEVNVVLLLSLVNDMFHGLY 44
           MMYTHH L+V FTGN +E F FAT+V+ V+ L LVND+ HGLY
Sbjct: 452 MMYTHHGLQVTFTGNFNEYFGFATDVDAVVYLMLVNDLIHGLY 494

[Close]

BLAST of Cucsa.169150 vs. Swiss-Prot
Match: GLGB1_PEA (1,4-alpha-glucan-branching enzyme 1, chloroplastic/amyloplastic OS=Pisum sativum GN=SBEI PE=1 SV=1)

HSP 1 Score: 62.0 bits (149), Expect = 2.7e-09
Identity = 31/62 (50.00%), Postives = 38/62 (61.29%), Query Frame = 1


Query: 1   MMYTHHDLEVGFTGNNSENFRFATEVNVVLLLSLVNDMFHGLYLK----------MPMVC 53
           MMYTHH L+V FTGN SE F  AT+V  V+ + LVND+ HGL+ +          MP  C
Sbjct: 499 MMYTHHGLQVSFTGNYSEYFGLATDVEAVVYMMLVNDLIHGLFPEAVSIGEDVSGMPTFC 558

[Close]

BLAST of Cucsa.169150 vs. TrEMBL
Match: A0A0A0LBA6_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_3G751970 PE=4 SV=1)

HSP 1 Score: 71.2 bits (173), Expect = 5.0e-10
Identity = 36/64 (56.25%), Postives = 42/64 (65.62%), Query Frame = 1


Query: 1   MMYTHHDLEVGFTGNNSENFRFATEVNVVLLLSLVNDMFHGLYLK----------MPMVC 55
           MMYTHH LEVGFTGN SE F FAT+V+ V+ L LVNDM HGLY +          MP  C
Sbjct: 494 MMYTHHGLEVGFTGNYSEYFGFATDVDAVVYLMLVNDMIHGLYPEAVTIGEDVSGMPTFC 553

[Close]

BLAST of Cucsa.169150 vs. TrEMBL
Match: B3U2B7_CUCSA (Starch branching enzyme I OS=Cucumis sativus PE=4 SV=1)

HSP 1 Score: 71.2 bits (173), Expect = 5.0e-10
Identity = 36/64 (56.25%), Postives = 42/64 (65.62%), Query Frame = 1


Query: 1   MMYTHHDLEVGFTGNNSENFRFATEVNVVLLLSLVNDMFHGLYLK----------MPMVC 55
           MMYTHH LEVGFTGN SE F FAT+V+ V+ L LVNDM HGLY +          MP  C
Sbjct: 523 MMYTHHGLEVGFTGNYSEYFGFATDVDAVVYLMLVNDMIHGLYPEAVTIGEDVSGMPTFC 582

[Close]

BLAST of Cucsa.169150 vs. TrEMBL
Match: B3U2C1_CUCSA (Starch branching enzyme I OS=Cucumis sativus PE=4 SV=1)

HSP 1 Score: 71.2 bits (173), Expect = 5.0e-10
Identity = 36/64 (56.25%), Postives = 42/64 (65.62%), Query Frame = 1


Query: 1   MMYTHHDLEVGFTGNNSENFRFATEVNVVLLLSLVNDMFHGLYLK----------MPMVC 55
           MMYTHH LEVGFTGN SE F FAT+V+ V+ L LVNDM HGLY +          MP  C
Sbjct: 523 MMYTHHGLEVGFTGNYSEYFGFATDVDAVVYLMLVNDMIHGLYPEAVTIGEDVSGMPTFC 582

[Close]

BLAST of Cucsa.169150 vs. TrEMBL
Match: A0A061ETN2_THECC (Starch branching enzyme 2.2 isoform 3 OS=Theobroma cacao GN=TCM_022491 PE=4 SV=1)

HSP 1 Score: 68.6 bits (166), Expect = 3.2e-09
Identity = 35/64 (54.69%), Postives = 41/64 (64.06%), Query Frame = 1


Query: 1   MMYTHHDLEVGFTGNNSENFRFATEVNVVLLLSLVNDMFHGLYLK----------MPMVC 55
           MMYTHH LEV FTGN +E F FAT+V+ V+ L LVNDM HGLY +          MP  C
Sbjct: 496 MMYTHHGLEVAFTGNYNEYFGFATDVDAVVYLMLVNDMIHGLYPEAVTIGEDVSGMPTFC 555

[Close]

BLAST of Cucsa.169150 vs. TrEMBL
Match: A0A061F0Y6_THECC (Starch branching enzyme 2.1 isoform 1 OS=Theobroma cacao GN=TCM_022491 PE=4 SV=1)

HSP 1 Score: 68.6 bits (166), Expect = 3.2e-09
Identity = 35/64 (54.69%), Postives = 41/64 (64.06%), Query Frame = 1


Query: 1   MMYTHHDLEVGFTGNNSENFRFATEVNVVLLLSLVNDMFHGLYLK----------MPMVC 55
           MMYTHH LEV FTGN +E F FAT+V+ V+ L LVNDM HGLY +          MP  C
Sbjct: 496 MMYTHHGLEVAFTGNYNEYFGFATDVDAVVYLMLVNDMIHGLYPEAVTIGEDVSGMPTFC 555

[Close]

BLAST of Cucsa.169150 vs. TAIR10
Match: AT2G36390.1 (AT2G36390.1 starch branching enzyme 2.1)

HSP 1 Score: 63.2 bits (152), Expect = 6.9e-11
Identity = 31/64 (48.44%), Postives = 41/64 (64.06%), Query Frame = 1


Query: 1   MMYTHHDLEVGFTGNNSENFRFATEVNVVLLLSLVNDMFHGLYLK----------MPMVC 55
           MMYTHH L+V FTGN +E F ++T+V+ V+ L LVND+ HGLY +          MP  C
Sbjct: 491 MMYTHHGLQVEFTGNYNEYFGYSTDVDAVVYLMLVNDLIHGLYPEAIVVGEDVSGMPAFC 550

[Close]

BLAST of Cucsa.169150 vs. TAIR10
Match: AT5G03650.1 (AT5G03650.1 starch branching enzyme 2.2)

HSP 1 Score: 62.4 bits (150), Expect = 1.2e-10
Identity = 32/64 (50.00%), Postives = 38/64 (59.38%), Query Frame = 1


Query: 1   MMYTHHDLEVGFTGNNSENFRFATEVNVVLLLSLVNDMFHGLYLK----------MPMVC 55
           MMYTHH L VGFTGN +E F   T+V+ V  L LVNDM HGLY +          MP  C
Sbjct: 456 MMYTHHGLSVGFTGNYTEYFGLETDVDAVNYLMLVNDMIHGLYPEAITVGEDVSGMPTFC 515

[Close]

BLAST of Cucsa.169150 vs. NCBI nr
Match: gi|168988220|gb|ACA35286.1| (starch branching enzyme I [Cucumis sativus])

HSP 1 Score: 71.2 bits (173), Expect = 7.2e-10
Identity = 36/64 (56.25%), Postives = 42/64 (65.62%), Query Frame = 1


Query: 1   MMYTHHDLEVGFTGNNSENFRFATEVNVVLLLSLVNDMFHGLYLK----------MPMVC 55
           MMYTHH LEVGFTGN SE F FAT+V+ V+ L LVNDM HGLY +          MP  C
Sbjct: 523 MMYTHHGLEVGFTGNYSEYFGFATDVDAVVYLMLVNDMIHGLYPEAVTIGEDVSGMPTFC 582

[Close]

BLAST of Cucsa.169150 vs. NCBI nr
Match: gi|449440211|ref|XP_004137878.1| (PREDICTED: 1,4-alpha-glucan-branching enzyme 2-2, chloroplastic/amyloplastic [Cucumis sativus])

HSP 1 Score: 71.2 bits (173), Expect = 7.2e-10
Identity = 36/64 (56.25%), Postives = 42/64 (65.62%), Query Frame = 1


Query: 1   MMYTHHDLEVGFTGNNSENFRFATEVNVVLLLSLVNDMFHGLYLK----------MPMVC 55
           MMYTHH LEVGFTGN SE F FAT+V+ V+ L LVNDM HGLY +          MP  C
Sbjct: 494 MMYTHHGLEVGFTGNYSEYFGFATDVDAVVYLMLVNDMIHGLYPEAVTIGEDVSGMPTFC 553

[Close]

BLAST of Cucsa.169150 vs. NCBI nr
Match: gi|659084292|ref|XP_008442810.1| (PREDICTED: 1,4-alpha-glucan-branching enzyme 2-2, chloroplastic/amyloplastic [Cucumis melo])

HSP 1 Score: 71.2 bits (173), Expect = 7.2e-10
Identity = 36/64 (56.25%), Postives = 42/64 (65.62%), Query Frame = 1


Query: 1   MMYTHHDLEVGFTGNNSENFRFATEVNVVLLLSLVNDMFHGLYLK----------MPMVC 55
           MMYTHH LEVGFTGN SE F FAT+V+ V+ L LVNDM HGLY +          MP  C
Sbjct: 494 MMYTHHGLEVGFTGNYSEYFGFATDVDAVVYLMLVNDMIHGLYPEAVTIGEDVSGMPTFC 553

[Close]

BLAST of Cucsa.169150 vs. NCBI nr
Match: gi|168988215|gb|ACA35282.1| (starch branching enzyme I [Cucumis sativus])

HSP 1 Score: 71.2 bits (173), Expect = 7.2e-10
Identity = 36/64 (56.25%), Postives = 42/64 (65.62%), Query Frame = 1


Query: 1   MMYTHHDLEVGFTGNNSENFRFATEVNVVLLLSLVNDMFHGLYLK----------MPMVC 55
           MMYTHH LEVGFTGN SE F FAT+V+ V+ L LVNDM HGLY +          MP  C
Sbjct: 523 MMYTHHGLEVGFTGNYSEYFGFATDVDAVVYLMLVNDMIHGLYPEAVTIGEDVSGMPTFC 582

[Close]

BLAST of Cucsa.169150 vs. NCBI nr
Match: gi|590631745|ref|XP_007027649.1| (Starch branching enzyme 2.2 isoform 3 [Theobroma cacao])

HSP 1 Score: 68.6 bits (166), Expect = 4.7e-09
Identity = 35/64 (54.69%), Postives = 41/64 (64.06%), Query Frame = 1


Query: 1   MMYTHHDLEVGFTGNNSENFRFATEVNVVLLLSLVNDMFHGLYLK----------MPMVC 55
           MMYTHH LEV FTGN +E F FAT+V+ V+ L LVNDM HGLY +          MP  C
Sbjct: 496 MMYTHHGLEVAFTGNYNEYFGFATDVDAVVYLMLVNDMIHGLYPEAVTIGEDVSGMPTFC 555

The following BLAST results are available for this feature:

BLAST of Cucsa.169150 vs. Swiss-Prot


Match Name	E-value	Identity	Description
GLGB1_ARATH	1.2e-09	48.44	1,4-alpha-glucan-branching enzyme 2-1, chloroplastic/amyloplastic OS=Arabidopsis...	[more]
GLGB2_ARATH	2.1e-09	50.00	1,4-alpha-glucan-branching enzyme 2-2, chloroplastic/amyloplastic OS=Arabidopsis...	[more]
GLGB_MAIZE	2.7e-09	65.12	1,4-alpha-glucan-branching enzyme 2, chloroplastic/amyloplastic OS=Zea mays GN=S...	[more]
GLGB1_PEA	2.7e-09	50.00	1,4-alpha-glucan-branching enzyme 1, chloroplastic/amyloplastic OS=Pisum sativum...	[more]

BLAST of Cucsa.169150 vs. TrEMBL


Match Name	E-value	Identity	Description
A0A0A0LBA6_CUCSA	5.0e-10	56.25	Uncharacterized protein OS=Cucumis sativus GN=Csa_3G751970 PE=4 SV=1	[more]
B3U2B7_CUCSA	5.0e-10	56.25	Starch branching enzyme I OS=Cucumis sativus PE=4 SV=1	[more]
B3U2C1_CUCSA	5.0e-10	56.25	Starch branching enzyme I OS=Cucumis sativus PE=4 SV=1	[more]
A0A061ETN2_THECC	3.2e-09	54.69	Starch branching enzyme 2.2 isoform 3 OS=Theobroma cacao GN=TCM_022491 PE=4 SV=1	[more]
A0A061F0Y6_THECC	3.2e-09	54.69	Starch branching enzyme 2.1 isoform 1 OS=Theobroma cacao GN=TCM_022491 PE=4 SV=1	[more]

BLAST of Cucsa.169150 vs. TAIR10


Match Name	E-value	Identity	Description
AT2G36390.1	6.9e-11	48.44	starch branching enzyme 2.1	[more]
AT5G03650.1	1.2e-10	50.00	starch branching enzyme 2.2	[more]

BLAST of Cucsa.169150 vs. NCBI nr


Match Name	E-value	Identity	Description
gi\|168988220\|gb\|ACA35286.1\|	7.2e-10	56.25	starch branching enzyme I [Cucumis sativus]	[more]
gi\|449440211\|ref\|XP_004137878.1\|	7.2e-10	56.25	PREDICTED: 1,4-alpha-glucan-branching enzyme 2-2, chloroplastic/amyloplastic [Cu...	[more]
gi\|659084292\|ref\|XP_008442810.1\|	7.2e-10	56.25	PREDICTED: 1,4-alpha-glucan-branching enzyme 2-2, chloroplastic/amyloplastic [Cu...	[more]
gi\|168988215\|gb\|ACA35282.1\|	7.2e-10	56.25	starch branching enzyme I [Cucumis sativus]	[more]
gi\|590631745\|ref\|XP_007027649.1\|	4.7e-09	54.69	Starch branching enzyme 2.2 isoform 3 [Theobroma cacao]	[more]

Annotated Terms

The following terms have been associated with this gene:

Vocabulary: **INTERPRO**
Term	Definition
IPR015902	Glycoside hydrolase, family 13

Vocabulary: **Molecular Function**
Term	Definition
GO:0003824	catalytic activity

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0008150	biological_process
cellular_component	GO:0005575	cellular_component
molecular_function	GO:0003824	catalytic activity

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Cucsa.169150.1	Cucsa.169150.1	mRNA

InterPro

Analysis Name: InterPro Annotations of cucumber (Gy14)
Date Performed: 2017-01-17


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR015902	Glycoside hydrolase, family 13	PANTHER	PTHR10357	ALPHA-AMYLASE	coord: 1..52 score: 2.2
None	No IPR available	PANTHER	PTHR10357:SF141	1,4-ALPHA-GLUCAN-BRANCHING ENZYME 2-1, CHLOROPLASTIC/AMYLOPLASTIC-RELATED	coord: 1..52 score: 2.2

Synteny

The following gene(s) are orthologous to this gene:

None

The following gene(s) are paralogous to this gene:

None

The following block(s) are covering this gene:


Gene	Organism	Block
Cucsa.169150	Cucurbita pepo (Zucchini)	cgycpeB0466
Cucsa.169150	Cucurbita pepo (Zucchini)	cgycpeB0467
Cucsa.169150	Bottle gourd (USVL1VR-Ls)	cgylsiB281
Cucsa.169150	Melon (DHL92) v3.6.1	cgymedB294
Cucsa.169150	Silver-seed gourd	carcgyB0409
Cucsa.169150	Silver-seed gourd	carcgyB0976
Cucsa.169150	Cucumber (Chinese Long) v3	cgycucB270
Cucsa.169150	Watermelon (97103) v2	cgywmbB307
Cucsa.169150	Wax gourd	cgywgoB348
Cucsa.169150	Cucumber (Gy14) v1	cgycgyB107
Cucsa.169150	Cucurbita maxima (Rimu)	cgycmaB0479
Cucsa.169150	Cucurbita maxima (Rimu)	cgycmaB0480
Cucsa.169150	Cucurbita moschata (Rifu)	cgycmoB0482
Cucsa.169150	Cucurbita moschata (Rifu)	cgycmoB0483
Cucsa.169150	Wild cucumber (PI 183967)	cgycpiB265
Cucsa.169150	Wild cucumber (PI 183967)	cgycpiB266
Cucsa.169150	Cucumber (Chinese Long) v2	cgycuB257
Cucsa.169150	Cucumber (Chinese Long) v2	cgycuB258
Cucsa.169150	Melon (DHL92) v3.5.1	cgymeB301
Cucsa.169150	Melon (DHL92) v3.5.1	cgymeB302
Cucsa.169150	Watermelon (Charleston Gray)	cgywcgB303
Cucsa.169150	Watermelon (97103) v1	cgywmB321
Cucsa.169150	Watermelon (97103) v1	cgywmB322