Cucsa.168270 (gene) Cucumber (Gy14) v1 | Cucurbit Genomics Database (CuGenDB)

Overview


Name	Cucsa.168270
Type	gene
Organism	Cucumis sativus (Cucumber (Gy14) v1)
Description	Homocysteine S-methyltransferase
Location	scaffold01160 : 28833 .. 33218 (-)

Genome Viewer

Gene Expression

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSthree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGCCCGAAACCGCCACCACCTTCCTTACCCATTTTTTGCAACACTCCGGCGGTTACGGGGTTCTAGACGGCGGCTTTGCCACTGAACTCGAGCGCCTCGGTGCCGATCTTAACGATCCTCTTTGGAGCGCCAAATGCCTCCTCACTTCCCCTCACCTTGTCCGACGGGTACCTTTCTTTTTTCTTTTTCAATCAAATCTTATCCATTTTATTCTTTACAGATTATTACAATCTCAATTTTACTCTTTATCTATTGTCTAATCTCTCAACTTTTTAAAACCTTTACTGTGTCGGGGATCTATTATGTGTAATAATATTCCAACATTTTGGACCATAAATATTTTCAAACTATATACCAAAATTTTTTATTCCATTCAAATGTATAGTTGTTTACCTTTGCCATTTACAGAAACTTTCTATTGTATTCAATAATATTGAAATGGTGTATTTTTTTGTTCTGAAAATTTTAACTTTTAATTTTCTTTATCTAATGATTTCAAATACATCCCTGACTTTTTCTGATATAAATTTAAATTTTAAGAATTTATTTTATACTTTGTAAAGTTTAAGGATGTAAATCTAGAATTAAAAAGCATATCAAAACGTTTTCTTTTTTAAAAAACTAAATTAGCTTTGTATTTTAATTTTTTTATTCTAAGTTCAAGAGCCTTTTTATTGTTCTGGAAAAATGGAACTTAAAACAGAAATGTTATTACCCAACGCATGACCTTTTAAAAAGAAAAAAATTAAAATCTAAATTAAATCACGATTTAAATATTTAAAAGAGAAGTGTCATAGTTCGTTTTGGGCCGGAGAATGGACTGGTTGTGAGAGGCCCAATATCAGAATAGGGCTCAGGTTAGCTAGCAGTCTCTTTCTTCCAAATGTGTGCGCCGCCACCTCTTTGAAATTATTTATTTAGGCTAAAAATATGTTCTTTATCGGACCCTTTTCAAATATAGAAAAAATTTACCACTGAATTCAGATGTAAATATTTCGTTTCTAAATATTTTTTTTGACAATTTTCCTTATTTATTTATGTCCAATTTTGTTGGTTCATATACTTTTAAACTTTGTTTAATTTTTACTAAATTTGATATTTTACTACTCAACCGTTTGAAATTAATTAAATATTGCAATAATTTTTATGAAAAAGAAAAATACAATATGTAAAGGGGAATCTTCAAAAATAGTAAATTTAACAAAATATTTATAACATACCAAAATTTCAGATTTTATAACTAATAAATATTGATAGGCACGGATATATTTATATATATTTTGCTATAGTTAGTAAATGTTTAAGTTTATTTTACTATATTTGAAAAAATATTTTTACAATTTACGGTGAAGATACCAATAAAAAATAAAATATAATTAAATAACTACCATAATTTAGTTGTAGAGACTAAATTTAAGATTTCCTTTTTCAATTTATCAACGTTTGTAAATTTCAAAGTAGTACACGTACATCTTAATTAAAATTTAAAACTTTGAAATTGTTCCAATAAGACTCTTTAATTAAAATGATGAATTAAAATTAACATAACATTTAAAACGTAGTTACCATATGTGAATTCATGAAATGATATTATGAATGATAATATTTTGAGTTGAATTGGATAGTTACTTTACAACTATCTTAATCTTCTACATTTTTTTAACACTCTGTGAACACTCATAAAAGAGTGGGGTATTTGATTAAACTTGAAGGGCTTGTTTGACAACTTGAATAATTTAATAGAGTCAGGTCAGGTGGGCTTGATTGGCTCACCAATAGTTACTACTATAGTTCAAAATGCAACAAGACTTTTCAGTTAGAGTCTAGTGTAAGAAAAATCAATCGTTCAGTTGATATGTTTATTTATTTTGAGAAACTTCATTTTCTTTATTTTGAAAGAATATAGTATAGAAATATTTCTTCATCATCAGCTAATCTGTTCAACTTATGGCAGGTTCATTTAGATTATCTTGATGCTGGGGCCAATATCATTTCCACAGCATCTTATCAGGTTATTTTTCACTGTTCTATTAAAACTATTTGCTAGTTTCTTGTAAATTATTCAAATTGTTACTACAACTGTACTTGAATATTAGTTTTGTTCATAAGATTCAATCTTTGTTGCTCTAGATCCAAAATGTCAAGTTGCTAGATTTAATCATTTGATATCCCATATTCAAGCAGTTGTAAGAAATCAATGATTTGATTTCATTGTACATTATATGCAGGCAACAATTCAGGGTTTTGAGGCTAAAGGATTTTCTAGAGATGAGAGTGAAAATTTGCTAAGAAAAAGTGTTGAAATTGCAATTGAAGCACGTGATATCTACCTTGAACGATGTACGAAAGATTCTTGGGATTTCACTGAAACTGGAGCGGGATCGAGGAGGCCAATTCTGATAGCCGCCTCGATTGGCAGCTACGGGGCTTATTTGGCTGATGGGTCTGAGTATAGGTGACCAAACTCTTCACACTTGGAAAGTCACTTCAAACAGATTCTTAAGTTTGAGTTTTATTGTCTGACAAATTTTCTTAGGAGAAAATGGGTTTAGCCTAAACCCCTTGTTCCCTTTTTCTGTTTTGTACAAGTATATATATCTCTAACTGTTGTACTGTTGTGTTGTATCGTGCTGTTTTACAGCGGAGAGTATGGTGATTCAGTTACTCTAGAAACACTGAAAGATTTCCACAGAAGAAGAGTGCAAATTCTGGCTAATGCAGGTGCTGACTTAATCGCATTTGAGACAATTCCAAACAAGTTGGAAGCTCAAGTAAGAAAATGTTGTCGAAAACTAGATGCCATTGTTGGTGTTCAGGGTTCCAAGTTTAACTTCTTGGTGCTGCTTATGTGGAACTTTCAGGCATATGCTGAGCTTCTTGATGAAGAGGGAATCGAAATTCCTGCTTGGTTTTCTTTCAACTCCAAGGATGGAATCAATGTGGTTAGTGGAGATTCTATATCTGACTGTGCCTCCATTGCAGATGCTTCAAAGCAAGTTGTTGCTGTTGGAATCAACTGTACTCCTCCCAGATATATTCATGGACTAATCTTGTCGATTCGGGAGGTAAATTCTGCTAATATACTTTTACGGCAGCATTCTCATCATCTTATCTGTCTCAATGAAGTTCGAATAAAACTTTGGCCACAAATTTCATCAAAATAAATTTGAGGATTCGGAGATGTATTCATGCTCTTAAAAATAGGAAAATTCTAAGTCGTAGCCACTTTTCTTCACTGCAAAAGGCTTCTGCTAAATGTCCTGTGATAAAATTAGTGGATATAAACACGAATTACATGGAAAGAAACCTTCAGATTTGTTCAGAATCTAAGTAAAATCTGGTTCATCTAATTGGCAGGTCACTGACAAACCAATTGTAGTTTATCCAAACAGTGGAGAATCTTATGATGGTGTGACCAAACGGTGGTTGGTGAGTTTTCTACTGCTTTTCTGAGTGACTTATGTAAACTTCACTATTTTGTTTCTTTGTACCGATAAACACTCTCTTTTAGTTCTGTTTGTCCTTTGAAAAATTCAGTCAGTTGTGTTAATTTCTGCAGAAATCTGATGGGATGATAGGGGAAGACTTTGTATCATATGTAAGAAAGTGGAAGGAAGCTGGAGCCTCTCTGTTTGGTGGGTGTTGCAGAACCACTCCAAACACAATCAGAGGCATAGCAAAAGCGCTTTCTTCTAAAACTTTTTGAGCTCTTTCCCCTCTATTTACAAGCTTTGGTATGTACCTACTATATGTATATCAGCCTTTTTTCAAATACTGCTGTTAGATAATTATGTGATGATATCCTTCGAAACGGTATAGTGGTCGAGCGTTTGTAAATATTAAAGACAGAACTGTTAATAACAAAAACTCTTTCTGTTACACATCTCCTAAAATGGTTAAAATACTTGATTTGACATGACATTTATTCTAAACAATCAAGATTTTCGAGTCCTAATTAAGTATGTCTCTTTTTTCTTTCACAGATGCCCATGTCTTTGATAAATGCTGAAGTTTTCACAAGCTTTTTCAAGATCATGCAATAATTTTGAGACTGATGGTGATTGATGGTTTACATATAGATAACCAACAAATGGAGGTCGTAAAACTAGAGAGGAAAAAGAGGAGCCTATAATGCTTGAAGACAAAAGCAAGCAGTAGAATTCTTAGTGTAGTGTGGGGATGAAGATATGCCAATGTTGTGTAGCTCCCACTTTTAAGTGCCTAATTACATCCTTAGGGTTTGGCTTTAGATTCTCTAAAAAGGGATATTAGAGCTAATGTTTTGGGTTTCATAACAAATATCATTTGATACCCAAGTCTGTTTGTCTTATTGGTAGATCCAAGAGGTTTCCATCAAAGTTCGAATCTTTTGTTTTATCATGTAC

mRNA sequence

ATGCCCGAAACCGCCACCACCTTCCTTACCCATTTTTTGCAACACTCCGGCGGTTACGGGGTTCTAGACGGCGGCTTTGCCACTGAACTCGAGCGCCTCGGTGCCGATCTTAACGATCCTCTTTGGAGCGCCAAATGCCTCCTCACTTCCCCTCACCTTGTCCGACGGGTTCATTTAGATTATCTTGATGCTGGGGCCAATATCATTTCCACAGCATCTTATCAGGCAACAATTCAGGGTTTTGAGGCTAAAGGATTTTCTAGAGATGAGAGTGAAAATTTGCTAAGAAAAAGTGTTGAAATTGCAATTGAAGCACGTGATATCTACCTTGAACGATGTACGAAAGATTCTTGGGATTTCACTGAAACTGGAGCGGGATCGAGGAGGCCAATTCTGATAGCCGCCTCGATTGGCAGCTACGGGGCTTATTTGGCTGATGGGTCTGAGTATAGCGGAGAGTATGGTGATTCAGTTACTCTAGAAACACTGAAAGATTTCCACAGAAGAAGAGTGCAAATTCTGGCTAATGCAGGTGCTGACTTAATCGCATTTGAGACAATTCCAAACAAGTTGGAAGCTCAAGCATATGCTGAGCTTCTTGATGAAGAGGGAATCGAAATTCCTGCTTGGTTTTCTTTCAACTCCAAGGATGGAATCAATGTGGTTAGTGGAGATTCTATATCTGACTGTGCCTCCATTGCAGATGCTTCAAAGCAAGTTGTTGCTGTTGGAATCAACTGTACTCCTCCCAGATATATTCATGGACTAATCTTGTCGATTCGGGAGGTCACTGACAAACCAATTGTAGTTTATCCAAACAGTGGAGAATCTTATGATGGTGTGACCAAACGGTGGTTGAAATCTGATGGGATGATAGGGGAAGACTTTGTATCATATGTAAGAAAGTGGAAGGAAGCTGGAGCCTCTCTGTTTGGTGGGTGTTGCAGAACCACTCCAAACACAATCAGAGGCATAGCAAAAGCGCTTTCTTCTAAAACTTTTTGAGCTCTTTCCCCTCTATTTACAAGCTTTGATGCCCATGTCTTTGATAAATGCTGAAGTTTTCACAAGCTTTTTCAAGATCATGCAATAATTTTGAGACTGATGGTGATTGATGGTTTACATATAGATAACCAACAAATGGAGGTCGTAAAACTAGAGAGGAAAAAGAGGAGCCTATAATGCTTGAAGACAAAAGCAAGCAGTAGAATTCTTAGTGTAGTGTGGGGATGAAGATATGCCAATGTTGTGTAGCTCCCACTTTTAAGTGCCTAATTACATCCTTAGGGTTTGGCTTTAGATTCTCTAAAAAGGGATATTAGAGCTAATGTTTTGGGTTTCATAACAAATATCATTTGATACCCAAGTCTGTTTGTCTTATTGGTAGATCCAAGAGGTTTCCATCAAAGTTCGAATCTTTTGTTTTATCATGTAC

Coding sequence (CDS)

ATGCCCGAAACCGCCACCACCTTCCTTACCCATTTTTTGCAACACTCCGGCGGTTACGGGGTTCTAGACGGCGGCTTTGCCACTGAACTCGAGCGCCTCGGTGCCGATCTTAACGATCCTCTTTGGAGCGCCAAATGCCTCCTCACTTCCCCTCACCTTGTCCGACGGGTTCATTTAGATTATCTTGATGCTGGGGCCAATATCATTTCCACAGCATCTTATCAGGCAACAATTCAGGGTTTTGAGGCTAAAGGATTTTCTAGAGATGAGAGTGAAAATTTGCTAAGAAAAAGTGTTGAAATTGCAATTGAAGCACGTGATATCTACCTTGAACGATGTACGAAAGATTCTTGGGATTTCACTGAAACTGGAGCGGGATCGAGGAGGCCAATTCTGATAGCCGCCTCGATTGGCAGCTACGGGGCTTATTTGGCTGATGGGTCTGAGTATAGCGGAGAGTATGGTGATTCAGTTACTCTAGAAACACTGAAAGATTTCCACAGAAGAAGAGTGCAAATTCTGGCTAATGCAGGTGCTGACTTAATCGCATTTGAGACAATTCCAAACAAGTTGGAAGCTCAAGCATATGCTGAGCTTCTTGATGAAGAGGGAATCGAAATTCCTGCTTGGTTTTCTTTCAACTCCAAGGATGGAATCAATGTGGTTAGTGGAGATTCTATATCTGACTGTGCCTCCATTGCAGATGCTTCAAAGCAAGTTGTTGCTGTTGGAATCAACTGTACTCCTCCCAGATATATTCATGGACTAATCTTGTCGATTCGGGAGGTCACTGACAAACCAATTGTAGTTTATCCAAACAGTGGAGAATCTTATGATGGTGTGACCAAACGGTGGTTGAAATCTGATGGGATGATAGGGGAAGACTTTGTATCATATGTAAGAAAGTGGAAGGAAGCTGGAGCCTCTCTGTTTGGTGGGTGTTGCAGAACCACTCCAAACACAATCAGAGGCATAGCAAAAGCGCTTTCTTCTAAAACTTTTTGA

Protein sequence

MPETATTFLTHFLQHSGGYGVLDGGFATELERLGADLNDPLWSAKCLLTSPHLVRRVHLDYLDAGANIISTASYQATIQGFEAKGFSRDESENLLRKSVEIAIEARDIYLERCTKDSWDFTETGAGSRRPILIAASIGSYGAYLADGSEYSGEYGDSVTLETLKDFHRRRVQILANAGADLIAFETIPNKLEAQAYAELLDEEGIEIPAWFSFNSKDGINVVSGDSISDCASIADASKQVVAVGINCTPPRYIHGLILSIREVTDKPIVVYPNSGESYDGVTKRWLKSDGMIGEDFVSYVRKWKEAGASLFGGCCRTTPNTIRGIAKALSSKTF*

Homology

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BLAST of Cucsa.168270 vs. Swiss-Prot
Match: HMT3_ARATH (Homocysteine S-methyltransferase 3 OS=Arabidopsis thaliana GN=HMT3 PE=1 SV=2)

HSP 1 Score: 508.4 bits (1308), Expect = 5.8e-143
Identity = 242/330 (73.33%), Postives = 283/330 (85.76%), Query Frame = 1


Query: 3   ETATTFLTHFLQHSGGYGVLDGGFATELERLGADLNDPLWSAKCLLTSPHLVRRVHLDYL 62
           E  ++ +T FL+  GGY V+DGGFATEL+R GAD+NDPLWSAKCL+TSPHLV +VHLDYL
Sbjct: 7   EETSSLMTDFLEKCGGYAVVDGGFATELQRHGADINDPLWSAKCLITSPHLVTKVHLDYL 66

Query: 63  DAGANIISTASYQATIQGFEAKGFSRDESENLLRKSVEIAIEARDIYLERCTKDSWDFTE 122
           ++GANII TASYQATIQGF AKG S  E+ENLLR+SVEI  EAR+I+  RCTK SWDF  
Sbjct: 67  ESGANIIITASYQATIQGFVAKGLSVGEAENLLRRSVEITYEAREIFYNRCTKGSWDFAY 126

Query: 123 TGAGSRRPILIAASIGSYGAYLADGSEYSGEYGDSVTLETLKDFHRRRVQILANAGADLI 182
            G  SRRPIL+AAS+GSYGAYLADGSEYSG YGDSV+ ETLKDFHRRRVQILA +GADLI
Sbjct: 127 AGKASRRPILVAASVGSYGAYLADGSEYSGIYGDSVSKETLKDFHRRRVQILAKSGADLI 186

Query: 183 AFETIPNKLEAQAYAELLDEEGIEIPAWFSFNSKDGINVVSGDSISDCASIADASKQVVA 242
           AFETIPNKLEA+AYA+LL+EE I+IPAWFSF SKDG++V  GDS+ +CA +AD+ K VVA
Sbjct: 187 AFETIPNKLEAEAYADLLEEEDIDIPAWFSFTSKDGVSVPRGDSVVECAKVADSCKNVVA 246

Query: 243 VGINCTPPRYIHGLILSIREVTDKPIVVYPNSGESYDGVTKRWLKSDGMIGEDFVSYVRK 302
           +GINCT PRYIH LI+S+R++T KPIVVYPNSGE YDG+ K+W+KS+G   EDFVSYV K
Sbjct: 247 IGINCTAPRYIHALIISLRQMTRKPIVVYPNSGEVYDGLNKKWIKSEGESEEDFVSYVSK 306

Query: 303 WKEAGASLFGGCCRTTPNTIRGIAKALSSK 333
           W++AGASLFGGCCRTTPNTIR IAK LS +
Sbjct: 307 WRDAGASLFGGCCRTTPNTIRAIAKVLSDE 336

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BLAST of Cucsa.168270 vs. Swiss-Prot
Match: HMT2_ARATH (Homocysteine S-methyltransferase 2 OS=Arabidopsis thaliana GN=HMT-2 PE=1 SV=1)

HSP 1 Score: 465.7 bits (1197), Expect = 4.3e-130
Identity = 227/322 (70.50%), Postives = 270/322 (83.85%), Query Frame = 1


Query: 12  FLQHSGGYGVLDGGFATELERLGADLNDPLWSAKCLLTSPHLVRRVHLDYLDAGANIIST 71
           FL+ +GGY V+DGG ATE ER GADLNDPLWSAKCL+TSPHL+  VHLDYL+AGA+IIS+
Sbjct: 12  FLKQTGGYAVIDGGLATEFERHGADLNDPLWSAKCLVTSPHLIHTVHLDYLEAGADIISS 71

Query: 72  ASYQATIQGFEAKGFSRDESENLLRKSVEIAIEARDIYLERC-TKDSWDFTETGAGSRRP 131
           ASYQATIQGFEAKGFSR+ESE+LL+KSVEIA EAR+ Y ++C T  S D        +RP
Sbjct: 72  ASYQATIQGFEAKGFSREESESLLKKSVEIATEARNSYYDKCGTSSSMDDKIL---KKRP 131

Query: 132 ILIAASIGSYGAYLADGSEYSGEYGDSVTLETLKDFHRRRVQILANAGADLIAFETIPNK 191
           IL+AAS+GSYGAYLADGSEYSG YGDS+TLE LKDFHRRR+Q+LA +GADLIAFETIPNK
Sbjct: 132 ILVAASVGSYGAYLADGSEYSGIYGDSITLEKLKDFHRRRLQVLAESGADLIAFETIPNK 191

Query: 192 LEAQAYAELLDEEGIEIPAWFSFNSKDGINVVSGDSISDCASIADASKQVVAVGINCTPP 251
           +EAQA+A+LL+E  ++IP WFSFNSKDG+NVVSGDSI +C SIA+  ++VVAVGINCTPP
Sbjct: 192 IEAQAFADLLEEGDVKIPGWFSFNSKDGVNVVSGDSIKECISIAENCEKVVAVGINCTPP 251

Query: 252 RYIHGLILSIREVTDKPIVVYPNSGESYDGVTKRWLKSDGMIGEDFVSYVRKWKEAGASL 311
           R+I GL+L I +VT KPI+VYPNSGESYD   K W+++ G+  EDFVSYV KW +AG SL
Sbjct: 252 RFIEGLVLEIEKVTSKPILVYPNSGESYDADRKEWVENTGVGDEDFVSYVEKWMDAGVSL 311

Query: 312 FGGCCRTTPNTIRGIAKALSSK 333
            GGCCRTTP TIR I K L ++
Sbjct: 312 LGGCCRTTPTTIRAIHKRLVNR 330

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BLAST of Cucsa.168270 vs. Swiss-Prot
Match: SMTA_BRAOT (Selenocysteine Se-methyltransferase OS=Brassica oleracea var. italica GN=SMT PE=1 SV=1)

HSP 1 Score: 463.0 bits (1190), Expect = 2.8e-129
Identity = 228/330 (69.09%), Postives = 270/330 (81.82%), Query Frame = 1


Query: 5   ATTF--LTHFLQHSGGYGVLDGGFATELERLGADLNDPLWSAKCLLTSPHLVRRVHLDYL 64
           A TF  +   L+ +GGY ++DGG ATELER GADLNDPLWSAKCLLTSPHL+  VHLDYL
Sbjct: 8   AETFYSMKELLKETGGYAIIDGGLATELERHGADLNDPLWSAKCLLTSPHLIHTVHLDYL 67

Query: 65  DAGANIISTASYQATIQGFEAKGFSRDESENLLRKSVEIAIEARDIYLERCTKDSWDFTE 124
           +AGA+IIS+ASYQATIQGFEAKG+S ++SE+LLRKSVEIA EAR  Y ++C  D     +
Sbjct: 68  EAGADIISSASYQATIQGFEAKGYSIEKSESLLRKSVEIACEARSTYYDKCKDDD----D 127

Query: 125 TGAGSRRPILIAASIGSYGAYLADGSEYSGEYGDSVTLETLKDFHRRRVQILANAGADLI 184
                +RPIL+AAS+GSYGA+LADGSEYSG YGD +TLETLKDFHRRRVQ+LA +GAD+I
Sbjct: 128 KKILKKRPILVAASVGSYGAFLADGSEYSGIYGDLITLETLKDFHRRRVQVLAESGADII 187

Query: 185 AFETIPNKLEAQAYAELLDEEGIEIPAWFSFNSKDGINVVSGDSISDCASIADASKQVVA 244
           AFETIPNKLEAQA+AELLDE   +IP WFSFNSKDG+NVVSGDSI +C +IA+A ++VVA
Sbjct: 188 AFETIPNKLEAQAFAELLDEGVAKIPGWFSFNSKDGVNVVSGDSIKECIAIAEACEKVVA 247

Query: 245 VGINCTPPRYIHGLILSIREVTDKPIVVYPNSGESYDGVTKRWLKSDGMIGEDFVSYVRK 304
           VGINCTPPR+I GL+L I +VT KPI+VYPNSGE YD   K W+++ G+  EDFVSYV K
Sbjct: 248 VGINCTPPRFIEGLVLEIAKVTSKPILVYPNSGERYDPERKEWVENTGVGNEDFVSYVEK 307

Query: 305 WKEAGASLFGGCCRTTPNTIRGIAKALSSK 333
           W +AG SL GGCCRTTP TIR I K L S+
Sbjct: 308 WMDAGVSLLGGCCRTTPTTIRAIHKRLVSR 333

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BLAST of Cucsa.168270 vs. Swiss-Prot
Match: SMTA_ASTBI (Selenocysteine methyltransferase OS=Astragalus bisulcatus GN=SMTA PE=1 SV=1)

HSP 1 Score: 450.7 bits (1158), Expect = 1.4e-125
Identity = 223/331 (67.37%), Postives = 268/331 (80.97%), Query Frame = 1


Query: 5   ATTFLTHFLQHSGGYGVLDGGFATELERLGADLNDPLWSAKCLLTSPHLVRRVHLDYLDA 64
           ++  +T FL  +G   V+ GG  TEL+R GADLNDPLWSAKCLL+ PHL+R+VHLDYL+ 
Sbjct: 2   SSPLITDFLHQAGRAAVIAGGLGTELQRHGADLNDPLWSAKCLLSCPHLIRQVHLDYLEN 61

Query: 65  GANIISTASYQATIQGFEAKGFSRDESENLLRKSVEIAIEARDIYLERCTKDSWDFTETG 124
           GA+II TASYQATIQGF+AKGFS +E E LLR+SVEIA EARD+Y +RC + S D    G
Sbjct: 62  GADIIITASYQATIQGFKAKGFSDEEGEALLRRSVEIAREARDLYYQRCAESSSD---NG 121

Query: 125 AGSR----RPILIAASIGSYGAYLADGSEYSGEYGDSVTLETLKDFHRRRVQILANAGAD 184
             SR    RPILIA S+GSYGAYLADGSE+SG YGD++  ETLKDFHRR+VQILA++G D
Sbjct: 122 DDSRILKQRPILIAGSVGSYGAYLADGSEFSGNYGDAIKSETLKDFHRRKVQILADSGVD 181

Query: 185 LIAFETIPNKLEAQAYAELLDEEGIEIPAWFSFNSKDGINVVSGDSISDCASIADASKQV 244
           L+AFE +PNKLEAQAYA+LL+EE I  PAWF+F SKDG NVVSGDSI +C SIA++  +V
Sbjct: 182 LLAFEAVPNKLEAQAYADLLEEENIITPAWFAFTSKDGNNVVSGDSIEECGSIAESCDKV 241

Query: 245 VAVGINCTPPRYIHGLILSIREVTDKPIVVYPNSGESYDGVTKRWLKSDGMIGEDFVSYV 304
           VAVGINCTPPR+IH LIL +++VT KPIV+YPNSGE+YD + K W ++ G+  EDFVSYV
Sbjct: 242 VAVGINCTPPRFIHDLILLLKKVTAKPIVIYPNSGETYDAIRKEWGQNSGVTDEDFVSYV 301

Query: 305 RKWKEAGASLFGGCCRTTPNTIRGIAKALSS 332
            KW E+GASL GGCCRTTP+TIRGI K LSS
Sbjct: 302 DKWCESGASLVGGCCRTTPDTIRGIYKILSS 329

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BLAST of Cucsa.168270 vs. Swiss-Prot
Match: HMT2_MAIZE (Homocysteine S-methyltransferase 2 OS=Zea mays GN=HMT-2 PE=2 SV=1)

HSP 1 Score: 447.2 bits (1149), Expect = 1.6e-124
Identity = 218/327 (66.67%), Postives = 267/327 (81.65%), Query Frame = 1


Query: 4   TATTFLTHFLQHSGGYGVLDGGFATELERLGADLNDPLWSAKCLLTSPHLVRRVHLDYLD 63
           +A   +  ++  +GG  VLDGG ATELE  GADLNDPLWSAKCLL+SPHL+R+VH+DYL+
Sbjct: 8   SAEEAVRRWVDAAGGRLVLDGGLATELEANGADLNDPLWSAKCLLSSPHLIRKVHMDYLE 67

Query: 64  AGANIISTASYQATIQGFEAKGFSRDESENLLRKSVEIAIEARDIYLERCTKDSWDFTET 123
           AGANII TASYQATIQGFE+KGFS+++SENLL KSVEIA+EAR+++L+       +  E 
Sbjct: 68  AGANIIITASYQATIQGFESKGFSKEQSENLLTKSVEIALEAREMFLK-------EHLEK 127

Query: 124 GAGSRRPILIAASIGSYGAYLADGSEYSGEYGDSVTLETLKDFHRRRVQILANAGADLIA 183
               + P+L+AAS+GSYGAYLADGSEYSG+YG++ T E LKDFHRRR+Q+LA AG DLIA
Sbjct: 128 STPIQHPVLVAASLGSYGAYLADGSEYSGDYGEAGTKEFLKDFHRRRLQVLAEAGPDLIA 187

Query: 184 FETIPNKLEAQAYAELLDEEGIEIPAWFSFNSKDGINVVSGDSISDCASIADASKQVVAV 243
           FETIPNKLEA+AY ELL+E  I IPAWFSFNSKDG+++VSGDS+ +C +IAD   +V AV
Sbjct: 188 FETIPNKLEAEAYVELLEECNINIPAWFSFNSKDGVHIVSGDSLIECTTIADKCAKVGAV 247

Query: 244 GINCTPPRYIHGLILSIREVTDKPIVVYPNSGESYDGVTKRWLKSDGMIGEDFVSYVRKW 303
           GINCTPPR+IHGLILSIR+VTDKPI++YPNSGE YDG  K W++S G+   DFVSYV +W
Sbjct: 248 GINCTPPRFIHGLILSIRKVTDKPILIYPNSGERYDGEKKEWVESTGVSDGDFVSYVNEW 307

Query: 304 KEAGASLFGGCCRTTPNTIRGIAKALS 331
            + GA L GGCCRTTPNTIR I + L+
Sbjct: 308 CKDGAVLIGGCCRTTPNTIRAIHRTLN 327

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BLAST of Cucsa.168270 vs. TrEMBL
Match: A0A0A0LKT7_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_2G223160 PE=4 SV=1)

HSP 1 Score: 670.6 bits (1729), Expect = 9.8e-190
Identity = 333/334 (99.70%), Postives = 334/334 (100.00%), Query Frame = 1


Query: 1   MPETATTFLTHFLQHSGGYGVLDGGFATELERLGADLNDPLWSAKCLLTSPHLVRRVHLD 60
           MPETATTFLTHFLQHSGGYGVLDGGFATELERLGADLNDPLWSAKCLLTSPHLVRRVHLD
Sbjct: 5   MPETATTFLTHFLQHSGGYGVLDGGFATELERLGADLNDPLWSAKCLLTSPHLVRRVHLD 64

Query: 61  YLDAGANIISTASYQATIQGFEAKGFSRDESENLLRKSVEIAIEARDIYLERCTKDSWDF 120
           YLDAGANIISTASYQATIQGFEAKGFSRDESENLLRKSV+IAIEARDIYLERCTKDSWDF
Sbjct: 65  YLDAGANIISTASYQATIQGFEAKGFSRDESENLLRKSVQIAIEARDIYLERCTKDSWDF 124

Query: 121 TETGAGSRRPILIAASIGSYGAYLADGSEYSGEYGDSVTLETLKDFHRRRVQILANAGAD 180
           TETGAGSRRPILIAASIGSYGAYLADGSEYSGEYGDSVTLETLKDFHRRRVQILANAGAD
Sbjct: 125 TETGAGSRRPILIAASIGSYGAYLADGSEYSGEYGDSVTLETLKDFHRRRVQILANAGAD 184

Query: 181 LIAFETIPNKLEAQAYAELLDEEGIEIPAWFSFNSKDGINVVSGDSISDCASIADASKQV 240
           LIAFETIPNKLEAQAYAELLDEEGIEIPAWFSFNSKDGINVVSGDSISDCASIADASKQV
Sbjct: 185 LIAFETIPNKLEAQAYAELLDEEGIEIPAWFSFNSKDGINVVSGDSISDCASIADASKQV 244

Query: 241 VAVGINCTPPRYIHGLILSIREVTDKPIVVYPNSGESYDGVTKRWLKSDGMIGEDFVSYV 300
           VAVGINCTPPRYIHGLILSIREVTDKPIVVYPNSGESYDGVTKRWLKSDGMIGEDFVSYV
Sbjct: 245 VAVGINCTPPRYIHGLILSIREVTDKPIVVYPNSGESYDGVTKRWLKSDGMIGEDFVSYV 304

Query: 301 RKWKEAGASLFGGCCRTTPNTIRGIAKALSSKTF 335
           RKWKEAGASLFGGCCRTTPNTIRGIAKALSSKTF
Sbjct: 305 RKWKEAGASLFGGCCRTTPNTIRGIAKALSSKTF 338

[Close]

BLAST of Cucsa.168270 vs. TrEMBL
Match: W9QQW0_9ROSA (Homocysteine S-methyltransferase 3 OS=Morus notabilis GN=L484_009098 PE=4 SV=1)

HSP 1 Score: 545.0 bits (1403), Expect = 6.2e-152
Identity = 257/330 (77.88%), Postives = 299/330 (90.61%), Query Frame = 1


Query: 3   ETATTFLTHFLQHSGGYGVLDGGFATELERLGADLNDPLWSAKCLLTSPHLVRRVHLDYL 62
           E  T+F+  F++  GGY V+DGGFATELER GADLNDPLWSAKCL++SPHL+RRVHLDYL
Sbjct: 7   ERTTSFMADFVEKCGGYAVVDGGFATELERHGADLNDPLWSAKCLVSSPHLIRRVHLDYL 66

Query: 63  DAGANIISTASYQATIQGFEAKGFSRDESENLLRKSVEIAIEARDIYLERCTKDSWDFTE 122
           DAGANII + SYQATIQGFEAKGFSR+E+E LL+KSVE+A EAR+IY +RC KDSWDF E
Sbjct: 67  DAGANIIISGSYQATIQGFEAKGFSREEAEALLKKSVELAYEAREIYYDRCAKDSWDFVE 126

Query: 123 TGAGSRRPILIAASIGSYGAYLADGSEYSGEYGDSVTLETLKDFHRRRVQILANAGADLI 182
           +G  +RRPIL+AAS+GSYGAYLADGSEYSG YGD+VTLE LKDFHRRRVQILAN+GADLI
Sbjct: 127 SGRSTRRPILVAASVGSYGAYLADGSEYSGNYGDAVTLEKLKDFHRRRVQILANSGADLI 186

Query: 183 AFETIPNKLEAQAYAELLDEEGIEIPAWFSFNSKDGINVVSGDSISDCASIADASKQVVA 242
           AFET PNKLEA+AYAELL+EEGI+IPAWFSF SKDGINVVSGDSI +CASIAD+ KQVVA
Sbjct: 187 AFETTPNKLEAKAYAELLEEEGIDIPAWFSFTSKDGINVVSGDSILECASIADSCKQVVA 246

Query: 243 VGINCTPPRYIHGLILSIREVTDKPIVVYPNSGESYDGVTKRWLKSDGMIGEDFVSYVRK 302
           VGINCTPPR+IH L+ SIR+V+ KP+V+YPNSGE+YD V+K+W++S+G++ EDFVSYV K
Sbjct: 247 VGINCTPPRFIHSLVSSIRKVSSKPVVIYPNSGETYDAVSKQWIRSNGVVDEDFVSYVEK 306

Query: 303 WKEAGASLFGGCCRTTPNTIRGIAKALSSK 333
           W+EAGASLFGGCCRTTPNTIR I+KALS K
Sbjct: 307 WQEAGASLFGGCCRTTPNTIRAISKALSEK 336

[Close]

BLAST of Cucsa.168270 vs. TrEMBL
Match: V4S391_9ROSI (Uncharacterized protein OS=Citrus clementina GN=CICLE_v10005328mg PE=4 SV=1)

HSP 1 Score: 535.0 bits (1377), Expect = 6.4e-149
Identity = 254/328 (77.44%), Postives = 299/328 (91.16%), Query Frame = 1


Query: 6   TTFLTHFLQHSGGYGVLDGGFATELERLGADLNDPLWSAKCLLTSPHLVRRVHLDYLDAG 65
           T+F+T FLQ  GGY V+DGGFATELER GADLNDPLWSAKCL++SPHLVR+VHLDYLDAG
Sbjct: 9   TSFMTDFLQKCGGYSVVDGGFATELERHGADLNDPLWSAKCLVSSPHLVRKVHLDYLDAG 68

Query: 66  ANIISTASYQATIQGFEAKGFSRDESENLLRKSVEIAIEARDIYLERCTKDSWDFTETGA 125
           ANII TASYQATIQGFEAKGFS +E+E LLR+SVEIA EAR+IY +RC KDSWDFT +G 
Sbjct: 69  ANIIITASYQATIQGFEAKGFSTEEAEALLRRSVEIACEAREIYYDRCMKDSWDFTGSGR 128

Query: 126 GSRRPILIAASIGSYGAYLADGSEYSGEYGDSVTLETLKDFHRRRVQILANAGADLIAFE 185
            S RP+L+AAS+GSYGAYLADGSEYSG+YGD+V+LETLK+FHRRRVQILAN+GADLIAFE
Sbjct: 129 ISSRPVLVAASVGSYGAYLADGSEYSGDYGDAVSLETLKEFHRRRVQILANSGADLIAFE 188

Query: 186 TIPNKLEAQAYAELLDEEGIEIPAWFSFNSKDGINVVSGDSISDCASIADASKQVVAVGI 245
           TIPNKLEA+AYAELL+EEGI IPAWFSFNSKDGINVVSGDSI +CASIAD+ +QVVAVGI
Sbjct: 189 TIPNKLEAKAYAELLEEEGITIPAWFSFNSKDGINVVSGDSILECASIADSCEQVVAVGI 248

Query: 246 NCTPPRYIHGLILSIREVTDKPIVVYPNSGESYDGVTKRWLKSDGMIGEDFVSYVRKWKE 305
           NCT PR+IHGLILS+R+VT KP+++YPNSGE+Y+   K+W++S G+  EDFVSY+ KW++
Sbjct: 249 NCTSPRFIHGLILSVRKVTSKPVIIYPNSGETYNAELKKWVESTGVRDEDFVSYIGKWRD 308

Query: 306 AGASLFGGCCRTTPNTIRGIAKALSSKT 334
           AGASLFGGCCRTTPNTI+ I++ LS+K+
Sbjct: 309 AGASLFGGCCRTTPNTIKAISRVLSNKS 336

[Close]

BLAST of Cucsa.168270 vs. TrEMBL
Match: B9RTM3_RICCO (5-methyltetrahydrofolate:homocysteine methyltransferase, putative OS=Ricinus communis GN=RCOM_0911480 PE=4 SV=1)

HSP 1 Score: 533.5 bits (1373), Expect = 1.9e-148
Identity = 255/327 (77.98%), Postives = 292/327 (89.30%), Query Frame = 1


Query: 7   TFLTHFLQHSGGYGVLDGGFATELERLGADLNDPLWSAKCLLTSPHLVRRVHLDYLDAGA 66
           +F++ FLQ  GGY V+DGGFATELER GADLNDPLWSAKCL++SPHLVRRVHLDY+DAGA
Sbjct: 11  SFMSDFLQKCGGYAVIDGGFATELERHGADLNDPLWSAKCLISSPHLVRRVHLDYIDAGA 70

Query: 67  NIISTASYQATIQGFEAKGFSRDESENLLRKSVEIAIEARDIYLERCTKDSWDFTETGAG 126
           NII TASYQATIQGFEAKG S +E+E LLR+SVEIA EAR+IY + CTK SWD  E G  
Sbjct: 71  NIILTASYQATIQGFEAKGLSTEEAEQLLRRSVEIACEAREIYYDNCTKGSWDLMEDGKM 130

Query: 127 SRRPILIAASIGSYGAYLADGSEYSGEYGDSVTLETLKDFHRRRVQILANAGADLIAFET 186
           SR P+L+AASIGSYGAYLADGSEYSG+YGD+V+++TLKDFHRRR+QILA +GADLIAFET
Sbjct: 131 SRHPVLVAASIGSYGAYLADGSEYSGDYGDAVSIQTLKDFHRRRLQILAKSGADLIAFET 190

Query: 187 IPNKLEAQAYAELLDEEGIEIPAWFSFNSKDGINVVSGDSISDCASIADASKQVVAVGIN 246
           IPNKLEA+AYAELL+EEGI IPAWFSFNSKDGINVVSGDSI +CASIAD+SKQVVAVGIN
Sbjct: 191 IPNKLEAKAYAELLEEEGINIPAWFSFNSKDGINVVSGDSILECASIADSSKQVVAVGIN 250

Query: 247 CTPPRYIHGLILSIREVTDKPIVVYPNSGESYDGVTKRWLKSDGMIGEDFVSYVRKWKEA 306
           CTPPR+IHGLILS+RE T KPIV+YPNSGE+YD   K+W+KS G   EDFVSY+ KW+EA
Sbjct: 251 CTPPRFIHGLILSMREATSKPIVIYPNSGETYDAALKQWVKSCGASDEDFVSYIGKWREA 310

Query: 307 GASLFGGCCRTTPNTIRGIAKALSSKT 334
           GASLFGGCCRTTPNTIR I + +S+K+
Sbjct: 311 GASLFGGCCRTTPNTIRAICRNISNKS 337

[Close]

BLAST of Cucsa.168270 vs. TrEMBL
Match: A0A067L836_JATCU (Uncharacterized protein OS=Jatropha curcas GN=JCGZ_02212 PE=4 SV=1)

HSP 1 Score: 526.6 bits (1355), Expect = 2.3e-146
Identity = 253/328 (77.13%), Postives = 294/328 (89.63%), Query Frame = 1


Query: 6   TTFLTHFLQHSGGYGVLDGGFATELERLGADLNDPLWSAKCLLTSPHLVRRVHLDYLDAG 65
           ++F++ FLQ  GGY V+DGGFATELER GADLNDPLWSAKCL++SPHLVR+VHLDYLDAG
Sbjct: 9   SSFMSDFLQKCGGYAVIDGGFATELERHGADLNDPLWSAKCLISSPHLVRKVHLDYLDAG 68

Query: 66  ANIISTASYQATIQGFEAKGFSRDESENLLRKSVEIAIEARDIYLERCTKDSWDFTETGA 125
           ANII TASYQATIQGFEAKG SR+E+E LLR+SVEIA EAR+IY + C+K SWDF E+G 
Sbjct: 69  ANIILTASYQATIQGFEAKGLSREEAELLLRRSVEIACEAREIYYDNCSKSSWDFMESGN 128

Query: 126 GSRRPILIAASIGSYGAYLADGSEYSGEYGDSVTLETLKDFHRRRVQILANAGADLIAFE 185
            +RRPIL+AASIGSYGAYLADGSEYSG YGDSV+L+TLKDFHRRR+QILA AGAD+IAFE
Sbjct: 129 VTRRPILVAASIGSYGAYLADGSEYSGNYGDSVSLQTLKDFHRRRLQILAKAGADIIAFE 188

Query: 186 TIPNKLEAQAYAELLDEEGIEIPAWFSFNSKDGINVVSGDSISDCASIADASKQVVAVGI 245
           TIPNKLEA+AYAELL+EE I+IPAWFSFNSKDGINVVSGDSIS+CASIAD+ KQ+VAVGI
Sbjct: 189 TIPNKLEAKAYAELLEEEDIKIPAWFSFNSKDGINVVSGDSISECASIADSCKQLVAVGI 248

Query: 246 NCTPPRYIHGLILSIREVTDKPIVVYPNSGESYDGVTKRWLKSDGMIGEDFVSYVRKWKE 305
           NCTPPR+I  LILSI +VT+KP+V+YPNSGE+YD   K W+KS G+  EDFVSY+ KW++
Sbjct: 249 NCTPPRFIRELILSIAKVTNKPVVIYPNSGETYDAERKIWVKSSGVTDEDFVSYIGKWRQ 308

Query: 306 AGASLFGGCCRTTPNTIRGIAKALSSKT 334
           AGASLFGGCCRTTPNTIR I++ LS  +
Sbjct: 309 AGASLFGGCCRTTPNTIRAISRLLSDNS 336

[Close]

BLAST of Cucsa.168270 vs. TAIR10
Match: AT3G22740.1 (AT3G22740.1 homocysteine S-methyltransferase 3)

HSP 1 Score: 508.4 bits (1308), Expect = 3.3e-144
Identity = 242/330 (73.33%), Postives = 283/330 (85.76%), Query Frame = 1


Query: 3   ETATTFLTHFLQHSGGYGVLDGGFATELERLGADLNDPLWSAKCLLTSPHLVRRVHLDYL 62
           E  ++ +T FL+  GGY V+DGGFATEL+R GAD+NDPLWSAKCL+TSPHLV +VHLDYL
Sbjct: 7   EETSSLMTDFLEKCGGYAVVDGGFATELQRHGADINDPLWSAKCLITSPHLVTKVHLDYL 66

Query: 63  DAGANIISTASYQATIQGFEAKGFSRDESENLLRKSVEIAIEARDIYLERCTKDSWDFTE 122
           ++GANII TASYQATIQGF AKG S  E+ENLLR+SVEI  EAR+I+  RCTK SWDF  
Sbjct: 67  ESGANIIITASYQATIQGFVAKGLSVGEAENLLRRSVEITYEAREIFYNRCTKGSWDFAY 126

Query: 123 TGAGSRRPILIAASIGSYGAYLADGSEYSGEYGDSVTLETLKDFHRRRVQILANAGADLI 182
            G  SRRPIL+AAS+GSYGAYLADGSEYSG YGDSV+ ETLKDFHRRRVQILA +GADLI
Sbjct: 127 AGKASRRPILVAASVGSYGAYLADGSEYSGIYGDSVSKETLKDFHRRRVQILAKSGADLI 186

Query: 183 AFETIPNKLEAQAYAELLDEEGIEIPAWFSFNSKDGINVVSGDSISDCASIADASKQVVA 242
           AFETIPNKLEA+AYA+LL+EE I+IPAWFSF SKDG++V  GDS+ +CA +AD+ K VVA
Sbjct: 187 AFETIPNKLEAEAYADLLEEEDIDIPAWFSFTSKDGVSVPRGDSVVECAKVADSCKNVVA 246

Query: 243 VGINCTPPRYIHGLILSIREVTDKPIVVYPNSGESYDGVTKRWLKSDGMIGEDFVSYVRK 302
           +GINCT PRYIH LI+S+R++T KPIVVYPNSGE YDG+ K+W+KS+G   EDFVSYV K
Sbjct: 247 IGINCTAPRYIHALIISLRQMTRKPIVVYPNSGEVYDGLNKKWIKSEGESEEDFVSYVSK 306

Query: 303 WKEAGASLFGGCCRTTPNTIRGIAKALSSK 333
           W++AGASLFGGCCRTTPNTIR IAK LS +
Sbjct: 307 WRDAGASLFGGCCRTTPNTIRAIAKVLSDE 336

[Close]

BLAST of Cucsa.168270 vs. TAIR10
Match: AT3G63250.1 (AT3G63250.1 homocysteine methyltransferase 2)

HSP 1 Score: 465.7 bits (1197), Expect = 2.4e-131
Identity = 227/322 (70.50%), Postives = 270/322 (83.85%), Query Frame = 1


Query: 12  FLQHSGGYGVLDGGFATELERLGADLNDPLWSAKCLLTSPHLVRRVHLDYLDAGANIIST 71
           FL+ +GGY V+DGG ATE ER GADLNDPLWSAKCL+TSPHL+  VHLDYL+AGA+IIS+
Sbjct: 12  FLKQTGGYAVIDGGLATEFERHGADLNDPLWSAKCLVTSPHLIHTVHLDYLEAGADIISS 71

Query: 72  ASYQATIQGFEAKGFSRDESENLLRKSVEIAIEARDIYLERC-TKDSWDFTETGAGSRRP 131
           ASYQATIQGFEAKGFSR+ESE+LL+KSVEIA EAR+ Y ++C T  S D        +RP
Sbjct: 72  ASYQATIQGFEAKGFSREESESLLKKSVEIATEARNSYYDKCGTSSSMDDKIL---KKRP 131

Query: 132 ILIAASIGSYGAYLADGSEYSGEYGDSVTLETLKDFHRRRVQILANAGADLIAFETIPNK 191
           IL+AAS+GSYGAYLADGSEYSG YGDS+TLE LKDFHRRR+Q+LA +GADLIAFETIPNK
Sbjct: 132 ILVAASVGSYGAYLADGSEYSGIYGDSITLEKLKDFHRRRLQVLAESGADLIAFETIPNK 191

Query: 192 LEAQAYAELLDEEGIEIPAWFSFNSKDGINVVSGDSISDCASIADASKQVVAVGINCTPP 251
           +EAQA+A+LL+E  ++IP WFSFNSKDG+NVVSGDSI +C SIA+  ++VVAVGINCTPP
Sbjct: 192 IEAQAFADLLEEGDVKIPGWFSFNSKDGVNVVSGDSIKECISIAENCEKVVAVGINCTPP 251

Query: 252 RYIHGLILSIREVTDKPIVVYPNSGESYDGVTKRWLKSDGMIGEDFVSYVRKWKEAGASL 311
           R+I GL+L I +VT KPI+VYPNSGESYD   K W+++ G+  EDFVSYV KW +AG SL
Sbjct: 252 RFIEGLVLEIEKVTSKPILVYPNSGESYDADRKEWVENTGVGDEDFVSYVEKWMDAGVSL 311

Query: 312 FGGCCRTTPNTIRGIAKALSSK 333
            GGCCRTTP TIR I K L ++
Sbjct: 312 LGGCCRTTPTTIRAIHKRLVNR 330

[Close]

BLAST of Cucsa.168270 vs. TAIR10
Match: AT3G25900.1 (AT3G25900.1 Homocysteine S-methyltransferase family protein)

HSP 1 Score: 360.1 bits (923), Expect = 1.4e-99
Identity = 178/330 (53.94%), Postives = 234/330 (70.91%), Query Frame = 1


Query: 3   ETATTFLTHFLQHSGGYGVLDGGFATELERLGADLNDPLWSAKCLLTSPHLVRRVHLDYL 62
           E  +  L   ++  GG  V+DGGFAT+LE  GA +NDPLWSA  L+ +P L++RVH++YL
Sbjct: 4   EKKSALLEDLIKKCGGCAVVDGGFATQLEIHGAAINDPLWSAVSLIKNPELIKRVHMEYL 63

Query: 63  DAGANIISTASYQATIQGFEAKGFSRDESENLLRKSVEIAIEARDIYLERCTKDSWDFTE 122
           +AGA+I+ T+SYQATI GF ++G S +ESE+LL+KSVE+A+EARD + E+ +K S     
Sbjct: 64  EAGADIVVTSSYQATIPGFLSRGLSIEESESLLQKSVELAVEARDRFWEKVSKVS----- 123

Query: 123 TGAGSRRPILIAASIGSYGAYLADGSEYSGEYGDSVTLETLKDFHRRRVQILANAGADLI 182
               S    L+AASIGSYGAYLADGSEYSG YG++V+L+ LKDFHRRR+Q+L  AG DL+
Sbjct: 124 --GHSYNRALVAASIGSYGAYLADGSEYSGHYGENVSLDKLKDFHRRRLQVLVEAGPDLL 183

Query: 183 AFETIPNKLEAQAYAELLDEEGIEIPAWFSFNSKDGINVVSGDSISDCASIADASKQVVA 242
           AFETIPNKLEAQA  ELL+EE ++IPAW  F S DG    SG+S  +C    + S  + A
Sbjct: 184 AFETIPNKLEAQACVELLEEEKVQIPAWICFTSVDGEKAPSGESFEECLEPLNKSNNIYA 243

Query: 243 VGINCTPPRYIHGLILSIREVTDKPIVVYPNSGESYDGVTKRWLKSDGMIGEDFVSYVRK 302
           VGINC PP++I  LI    ++T K IVVYPNSGE +DG  K+WL S     ++F  +  K
Sbjct: 244 VGINCAPPQFIENLIRKFAKLTKKAIVVYPNSGEVWDGKAKQWLPSQCFGDDEFEMFATK 303

Query: 303 WKEAGASLFGGCCRTTPNTIRGIAKALSSK 333
           W++ GA L GGCCRTTP+TI  I++ L  +
Sbjct: 304 WRDLGAKLIGGCCRTTPSTINAISRDLKRR 326

[Close]

BLAST of Cucsa.168270 vs. NCBI nr
Match: gi|449455288|ref|XP_004145385.1| (PREDICTED: homocysteine S-methyltransferase 3 [Cucumis sativus])

HSP 1 Score: 670.6 bits (1729), Expect = 1.4e-189
Identity = 333/334 (99.70%), Postives = 334/334 (100.00%), Query Frame = 1


Query: 1   MPETATTFLTHFLQHSGGYGVLDGGFATELERLGADLNDPLWSAKCLLTSPHLVRRVHLD 60
           MPETATTFLTHFLQHSGGYGVLDGGFATELERLGADLNDPLWSAKCLLTSPHLVRRVHLD
Sbjct: 5   MPETATTFLTHFLQHSGGYGVLDGGFATELERLGADLNDPLWSAKCLLTSPHLVRRVHLD 64

Query: 61  YLDAGANIISTASYQATIQGFEAKGFSRDESENLLRKSVEIAIEARDIYLERCTKDSWDF 120
           YLDAGANIISTASYQATIQGFEAKGFSRDESENLLRKSV+IAIEARDIYLERCTKDSWDF
Sbjct: 65  YLDAGANIISTASYQATIQGFEAKGFSRDESENLLRKSVQIAIEARDIYLERCTKDSWDF 124

Query: 121 TETGAGSRRPILIAASIGSYGAYLADGSEYSGEYGDSVTLETLKDFHRRRVQILANAGAD 180
           TETGAGSRRPILIAASIGSYGAYLADGSEYSGEYGDSVTLETLKDFHRRRVQILANAGAD
Sbjct: 125 TETGAGSRRPILIAASIGSYGAYLADGSEYSGEYGDSVTLETLKDFHRRRVQILANAGAD 184

Query: 181 LIAFETIPNKLEAQAYAELLDEEGIEIPAWFSFNSKDGINVVSGDSISDCASIADASKQV 240
           LIAFETIPNKLEAQAYAELLDEEGIEIPAWFSFNSKDGINVVSGDSISDCASIADASKQV
Sbjct: 185 LIAFETIPNKLEAQAYAELLDEEGIEIPAWFSFNSKDGINVVSGDSISDCASIADASKQV 244

Query: 241 VAVGINCTPPRYIHGLILSIREVTDKPIVVYPNSGESYDGVTKRWLKSDGMIGEDFVSYV 300
           VAVGINCTPPRYIHGLILSIREVTDKPIVVYPNSGESYDGVTKRWLKSDGMIGEDFVSYV
Sbjct: 245 VAVGINCTPPRYIHGLILSIREVTDKPIVVYPNSGESYDGVTKRWLKSDGMIGEDFVSYV 304

Query: 301 RKWKEAGASLFGGCCRTTPNTIRGIAKALSSKTF 335
           RKWKEAGASLFGGCCRTTPNTIRGIAKALSSKTF
Sbjct: 305 RKWKEAGASLFGGCCRTTPNTIRGIAKALSSKTF 338

[Close]

BLAST of Cucsa.168270 vs. NCBI nr
Match: gi|659096876|ref|XP_008449335.1| (PREDICTED: homocysteine S-methyltransferase 3 [Cucumis melo])

HSP 1 Score: 653.3 bits (1684), Expect = 2.3e-184
Identity = 322/334 (96.41%), Postives = 332/334 (99.40%), Query Frame = 1


Query: 1   MPETATTFLTHFLQHSGGYGVLDGGFATELERLGADLNDPLWSAKCLLTSPHLVRRVHLD 60
           MPETATT+LTHFLQ  GGYGVLDGGFATELERLGADLNDPLWSAKCLLTSPHLVRRVHLD
Sbjct: 5   MPETATTYLTHFLQKCGGYGVLDGGFATELERLGADLNDPLWSAKCLLTSPHLVRRVHLD 64

Query: 61  YLDAGANIISTASYQATIQGFEAKGFSRDESENLLRKSVEIAIEARDIYLERCTKDSWDF 120
           YLDAGANIISTASYQAT+QGFEAKGFSRDESE+LLRKSV+IAIEARDIYLERCTKDSWDF
Sbjct: 65  YLDAGANIISTASYQATLQGFEAKGFSRDESEDLLRKSVQIAIEARDIYLERCTKDSWDF 124

Query: 121 TETGAGSRRPILIAASIGSYGAYLADGSEYSGEYGDSVTLETLKDFHRRRVQILANAGAD 180
           TETGAGSRRP+LIAASIGSYGAYLADGSEYSGEYG+SVTLETLK+FHRRRVQILANAGAD
Sbjct: 125 TETGAGSRRPVLIAASIGSYGAYLADGSEYSGEYGESVTLETLKNFHRRRVQILANAGAD 184

Query: 181 LIAFETIPNKLEAQAYAELLDEEGIEIPAWFSFNSKDGINVVSGDSISDCASIADASKQV 240
           LIAFETIPNKLEAQAYAELLDEEGIEIPAWFSFNSKDGINVVSGDSISDCASIADASKQV
Sbjct: 185 LIAFETIPNKLEAQAYAELLDEEGIEIPAWFSFNSKDGINVVSGDSISDCASIADASKQV 244

Query: 241 VAVGINCTPPRYIHGLILSIREVTDKPIVVYPNSGESYDGVTKRWLKSDGMIGEDFVSYV 300
           VAVGINCTPPRYIHGLILSI+EVTDKPIVVYPNSGE+YDGVTK+WLKSDGMIGEDFVSYV
Sbjct: 245 VAVGINCTPPRYIHGLILSIQEVTDKPIVVYPNSGETYDGVTKQWLKSDGMIGEDFVSYV 304

Query: 301 RKWKEAGASLFGGCCRTTPNTIRGIAKALSSKTF 335
           RKWKEAGASLFGGCCRTTPNTIRGIAKALSSKTF
Sbjct: 305 RKWKEAGASLFGGCCRTTPNTIRGIAKALSSKTF 338

[Close]

BLAST of Cucsa.168270 vs. NCBI nr
Match: gi|703084222|ref|XP_010092416.1| (Homocysteine S-methyltransferase 3 [Morus notabilis])

HSP 1 Score: 545.0 bits (1403), Expect = 8.9e-152
Identity = 257/330 (77.88%), Postives = 299/330 (90.61%), Query Frame = 1


Query: 3   ETATTFLTHFLQHSGGYGVLDGGFATELERLGADLNDPLWSAKCLLTSPHLVRRVHLDYL 62
           E  T+F+  F++  GGY V+DGGFATELER GADLNDPLWSAKCL++SPHL+RRVHLDYL
Sbjct: 7   ERTTSFMADFVEKCGGYAVVDGGFATELERHGADLNDPLWSAKCLVSSPHLIRRVHLDYL 66

Query: 63  DAGANIISTASYQATIQGFEAKGFSRDESENLLRKSVEIAIEARDIYLERCTKDSWDFTE 122
           DAGANII + SYQATIQGFEAKGFSR+E+E LL+KSVE+A EAR+IY +RC KDSWDF E
Sbjct: 67  DAGANIIISGSYQATIQGFEAKGFSREEAEALLKKSVELAYEAREIYYDRCAKDSWDFVE 126

Query: 123 TGAGSRRPILIAASIGSYGAYLADGSEYSGEYGDSVTLETLKDFHRRRVQILANAGADLI 182
           +G  +RRPIL+AAS+GSYGAYLADGSEYSG YGD+VTLE LKDFHRRRVQILAN+GADLI
Sbjct: 127 SGRSTRRPILVAASVGSYGAYLADGSEYSGNYGDAVTLEKLKDFHRRRVQILANSGADLI 186

Query: 183 AFETIPNKLEAQAYAELLDEEGIEIPAWFSFNSKDGINVVSGDSISDCASIADASKQVVA 242
           AFET PNKLEA+AYAELL+EEGI+IPAWFSF SKDGINVVSGDSI +CASIAD+ KQVVA
Sbjct: 187 AFETTPNKLEAKAYAELLEEEGIDIPAWFSFTSKDGINVVSGDSILECASIADSCKQVVA 246

Query: 243 VGINCTPPRYIHGLILSIREVTDKPIVVYPNSGESYDGVTKRWLKSDGMIGEDFVSYVRK 302
           VGINCTPPR+IH L+ SIR+V+ KP+V+YPNSGE+YD V+K+W++S+G++ EDFVSYV K
Sbjct: 247 VGINCTPPRFIHSLVSSIRKVSSKPVVIYPNSGETYDAVSKQWIRSNGVVDEDFVSYVEK 306

Query: 303 WKEAGASLFGGCCRTTPNTIRGIAKALSSK 333
           W+EAGASLFGGCCRTTPNTIR I+KALS K
Sbjct: 307 WQEAGASLFGGCCRTTPNTIRAISKALSEK 336

[Close]

BLAST of Cucsa.168270 vs. NCBI nr
Match: gi|567853760|ref|XP_006420000.1| (hypothetical protein CICLE_v10005328mg [Citrus clementina])

HSP 1 Score: 535.0 bits (1377), Expect = 9.2e-149
Identity = 254/328 (77.44%), Postives = 299/328 (91.16%), Query Frame = 1


Query: 6   TTFLTHFLQHSGGYGVLDGGFATELERLGADLNDPLWSAKCLLTSPHLVRRVHLDYLDAG 65
           T+F+T FLQ  GGY V+DGGFATELER GADLNDPLWSAKCL++SPHLVR+VHLDYLDAG
Sbjct: 9   TSFMTDFLQKCGGYSVVDGGFATELERHGADLNDPLWSAKCLVSSPHLVRKVHLDYLDAG 68

Query: 66  ANIISTASYQATIQGFEAKGFSRDESENLLRKSVEIAIEARDIYLERCTKDSWDFTETGA 125
           ANII TASYQATIQGFEAKGFS +E+E LLR+SVEIA EAR+IY +RC KDSWDFT +G 
Sbjct: 69  ANIIITASYQATIQGFEAKGFSTEEAEALLRRSVEIACEAREIYYDRCMKDSWDFTGSGR 128

Query: 126 GSRRPILIAASIGSYGAYLADGSEYSGEYGDSVTLETLKDFHRRRVQILANAGADLIAFE 185
            S RP+L+AAS+GSYGAYLADGSEYSG+YGD+V+LETLK+FHRRRVQILAN+GADLIAFE
Sbjct: 129 ISSRPVLVAASVGSYGAYLADGSEYSGDYGDAVSLETLKEFHRRRVQILANSGADLIAFE 188

Query: 186 TIPNKLEAQAYAELLDEEGIEIPAWFSFNSKDGINVVSGDSISDCASIADASKQVVAVGI 245
           TIPNKLEA+AYAELL+EEGI IPAWFSFNSKDGINVVSGDSI +CASIAD+ +QVVAVGI
Sbjct: 189 TIPNKLEAKAYAELLEEEGITIPAWFSFNSKDGINVVSGDSILECASIADSCEQVVAVGI 248

Query: 246 NCTPPRYIHGLILSIREVTDKPIVVYPNSGESYDGVTKRWLKSDGMIGEDFVSYVRKWKE 305
           NCT PR+IHGLILS+R+VT KP+++YPNSGE+Y+   K+W++S G+  EDFVSY+ KW++
Sbjct: 249 NCTSPRFIHGLILSVRKVTSKPVIIYPNSGETYNAELKKWVESTGVRDEDFVSYIGKWRD 308

Query: 306 AGASLFGGCCRTTPNTIRGIAKALSSKT 334
           AGASLFGGCCRTTPNTI+ I++ LS+K+
Sbjct: 309 AGASLFGGCCRTTPNTIKAISRVLSNKS 336

[Close]

BLAST of Cucsa.168270 vs. NCBI nr
Match: gi|255552095|ref|XP_002517092.1| (PREDICTED: homocysteine S-methyltransferase 3 [Ricinus communis])

HSP 1 Score: 533.5 bits (1373), Expect = 2.7e-148
Identity = 255/327 (77.98%), Postives = 292/327 (89.30%), Query Frame = 1


Query: 7   TFLTHFLQHSGGYGVLDGGFATELERLGADLNDPLWSAKCLLTSPHLVRRVHLDYLDAGA 66
           +F++ FLQ  GGY V+DGGFATELER GADLNDPLWSAKCL++SPHLVRRVHLDY+DAGA
Sbjct: 11  SFMSDFLQKCGGYAVIDGGFATELERHGADLNDPLWSAKCLISSPHLVRRVHLDYIDAGA 70

Query: 67  NIISTASYQATIQGFEAKGFSRDESENLLRKSVEIAIEARDIYLERCTKDSWDFTETGAG 126
           NII TASYQATIQGFEAKG S +E+E LLR+SVEIA EAR+IY + CTK SWD  E G  
Sbjct: 71  NIILTASYQATIQGFEAKGLSTEEAEQLLRRSVEIACEAREIYYDNCTKGSWDLMEDGKM 130

Query: 127 SRRPILIAASIGSYGAYLADGSEYSGEYGDSVTLETLKDFHRRRVQILANAGADLIAFET 186
           SR P+L+AASIGSYGAYLADGSEYSG+YGD+V+++TLKDFHRRR+QILA +GADLIAFET
Sbjct: 131 SRHPVLVAASIGSYGAYLADGSEYSGDYGDAVSIQTLKDFHRRRLQILAKSGADLIAFET 190

Query: 187 IPNKLEAQAYAELLDEEGIEIPAWFSFNSKDGINVVSGDSISDCASIADASKQVVAVGIN 246
           IPNKLEA+AYAELL+EEGI IPAWFSFNSKDGINVVSGDSI +CASIAD+SKQVVAVGIN
Sbjct: 191 IPNKLEAKAYAELLEEEGINIPAWFSFNSKDGINVVSGDSILECASIADSSKQVVAVGIN 250

Query: 247 CTPPRYIHGLILSIREVTDKPIVVYPNSGESYDGVTKRWLKSDGMIGEDFVSYVRKWKEA 306
           CTPPR+IHGLILS+RE T KPIV+YPNSGE+YD   K+W+KS G   EDFVSY+ KW+EA
Sbjct: 251 CTPPRFIHGLILSMREATSKPIVIYPNSGETYDAALKQWVKSCGASDEDFVSYIGKWREA 310

Query: 307 GASLFGGCCRTTPNTIRGIAKALSSKT 334
           GASLFGGCCRTTPNTIR I + +S+K+
Sbjct: 311 GASLFGGCCRTTPNTIRAICRNISNKS 337

The following BLAST results are available for this feature:

BLAST of Cucsa.168270 vs. Swiss-Prot


Match Name	E-value	Identity	Description
HMT3_ARATH	5.8e-143	73.33	Homocysteine S-methyltransferase 3 OS=Arabidopsis thaliana GN=HMT3 PE=1 SV=2	[more]
HMT2_ARATH	4.3e-130	70.50	Homocysteine S-methyltransferase 2 OS=Arabidopsis thaliana GN=HMT-2 PE=1 SV=1	[more]
SMTA_BRAOT	2.8e-129	69.09	Selenocysteine Se-methyltransferase OS=Brassica oleracea var. italica GN=SMT PE=...	[more]
SMTA_ASTBI	1.4e-125	67.37	Selenocysteine methyltransferase OS=Astragalus bisulcatus GN=SMTA PE=1 SV=1	[more]
HMT2_MAIZE	1.6e-124	66.67	Homocysteine S-methyltransferase 2 OS=Zea mays GN=HMT-2 PE=2 SV=1	[more]

BLAST of Cucsa.168270 vs. TrEMBL


Match Name	E-value	Identity	Description
A0A0A0LKT7_CUCSA	9.8e-190	99.70	Uncharacterized protein OS=Cucumis sativus GN=Csa_2G223160 PE=4 SV=1	[more]
W9QQW0_9ROSA	6.2e-152	77.88	Homocysteine S-methyltransferase 3 OS=Morus notabilis GN=L484_009098 PE=4 SV=1	[more]
V4S391_9ROSI	6.4e-149	77.44	Uncharacterized protein OS=Citrus clementina GN=CICLE_v10005328mg PE=4 SV=1	[more]
B9RTM3_RICCO	1.9e-148	77.98	5-methyltetrahydrofolate:homocysteine methyltransferase, putative OS=Ricinus com...	[more]
A0A067L836_JATCU	2.3e-146	77.13	Uncharacterized protein OS=Jatropha curcas GN=JCGZ_02212 PE=4 SV=1	[more]

BLAST of Cucsa.168270 vs. TAIR10


Match Name	E-value	Identity	Description
AT3G22740.1	3.3e-144	73.33	homocysteine S-methyltransferase 3	[more]
AT3G63250.1	2.4e-131	70.50	homocysteine methyltransferase 2	[more]
AT3G25900.1	1.4e-99	53.94	Homocysteine S-methyltransferase family protein	[more]

BLAST of Cucsa.168270 vs. NCBI nr


Match Name	E-value	Identity	Description
gi\|449455288\|ref\|XP_004145385.1\|	1.4e-189	99.70	PREDICTED: homocysteine S-methyltransferase 3 [Cucumis sativus]	[more]
gi\|659096876\|ref\|XP_008449335.1\|	2.3e-184	96.41	PREDICTED: homocysteine S-methyltransferase 3 [Cucumis melo]	[more]
gi\|703084222\|ref\|XP_010092416.1\|	8.9e-152	77.88	Homocysteine S-methyltransferase 3 [Morus notabilis]	[more]
gi\|567853760\|ref\|XP_006420000.1\|	9.2e-149	77.44	hypothetical protein CICLE_v10005328mg [Citrus clementina]	[more]
gi\|255552095\|ref\|XP_002517092.1\|	2.7e-148	77.98	PREDICTED: homocysteine S-methyltransferase 3 [Ricinus communis]	[more]

Annotated Terms

The following terms have been associated with this gene:

Vocabulary: **INTERPRO**
Term	Definition
IPR003726	HCY_dom
IPR003726	HCY_dom
IPR003726	HCY_dom

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006555	methionine metabolic process
biological_process	GO:0032259	methylation
cellular_component	GO:0005886	plasma membrane
molecular_function	GO:0008898	S-adenosylmethionine-homocysteine S-methyltransferase activity

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Cucsa.168270.3	Cucsa.168270.3	mRNA
Cucsa.168270.2	Cucsa.168270.2	mRNA
Cucsa.168270.1	Cucsa.168270.1	mRNA

InterPro

Analysis Name: InterPro Annotations of cucumber (Gy14)
Date Performed: 2017-01-17


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR003726	Homocysteine-binding domain	GENE3D	G3DSA:3.20.20.330		coord: 18..331 score: 6.2E
IPR003726	Homocysteine-binding domain	PFAM	PF02574	S-methyl_trans	coord: 21..329 score: 6.0
IPR003726	Homocysteine-binding domain	PROFILE	PS50970	HCY	coord: 8..329 score: 48
IPR003726	Homocysteine-binding domain	unknown	SSF82282	Homocysteine S-methyltransferase	coord: 16..331 score: 2.22
None	No IPR available	PANTHER	PTHR21091	METHYLTETRAHYDROFOLATE:HOMOCYSTEINE METHYLTRANSFERASE RELATED	coord: 1..330 score: 9.5E
None	No IPR available	PANTHER	PTHR21091:SF114	HOMOCYSTEINE S-METHYLTRANSFERASE 3	coord: 1..330 score: 9.5E

Synteny

The following gene(s) are orthologous to this gene:


Gene	Orthologue	Organism	Block
Cucsa.168270	Csa2G223160	Cucumber (Chinese Long) v2	cgycuB256
Cucsa.168270	MELO3C014294	Melon (DHL92) v3.5.1	cgymeB300
Cucsa.168270	CSPI02G11090	Wild cucumber (PI 183967)	cgycpiB264
Cucsa.168270	MELO3C014294.2	Melon (DHL92) v3.6.1	cgymedB293
Cucsa.168270	CsaV3_2G013530	Cucumber (Chinese Long) v3	cgycucB269

The following gene(s) are paralogous to this gene:

None

The following block(s) are covering this gene:

None