Cucsa.166850 (gene) Cucumber (Gy14) v1

BLAST of Cucsa.166850 vs. Swiss-Prot
Match: THCAS_CANSA (Tetrahydrocannabinolic acid synthase OS=Cannabis sativa PE=1 SV=1)

HSP 1 Score: 525.4 bits (1352), Expect = 7.4e-148
Identity = 260/515 (50.49%), Postives = 365/515 (70.87%), Query Frame = 1

Query: 29  QDNFLECFNSTSYSKHSIPISEVVFTNESASFSSLLRLSIRNLRFLTTTLPKPLLLVTPF 88
           ++NFL+CF+   +  +++   ++V+T     + S+L  +I+NLRF++ T PKPL++VTP 
Sbjct: 31  RENFLKCFSK--HIPNNVANPKLVYTQHDQLYMSILNSTIQNLRFISDTTPKPLVIVTPS 90

Query: 89  HESHVQAAILCAREKGLQVRVRSGGHDYEGLSYISSQAPFIVIDLINLRSIKIDIKTETA 148
           + SH+QA ILC+++ GLQ+R RSGGHD EG+SYIS Q PF+V+DL N+ SIKID+ ++TA
Sbjct: 91  NNSHIQATILCSKKVGLQIRTRSGGHDAEGMSYIS-QVPFVVVDLRNMHSIKIDVHSQTA 150

Query: 149 SVETGASLGELYYRIAKKSSIHGFPAGSCPTVGVGGHISGGGFGTLFRKYGLAADNVIDA 208
            VE GA+LGE+YY I +K+    FP G CPTVGVGGH SGGG+G L R YGLAADN+IDA
Sbjct: 151 WVEAGATLGEVYYWINEKNENLSFPGGYCPTVGVGGHFSGGGYGALMRNYGLAADNIIDA 210

Query: 209 KIIDFNGRIMDRSSMGEDLFWAIRGGGGASFGVILSWKLKLVSVPSIVTVFNVQRTLE-E 268
            +++ +G+++DR SMGEDLFWAIRGGGG +FG+I +WK+KLV+VPS  T+F+V++ +E  
Sbjct: 211 HLVNVDGKVLDRKSMGEDLFWAIRGGGGENFGIIAAWKIKLVAVPSKSTIFSVKKNMEIH 270

Query: 269 GATHLFQKWQNISHKLDQDIFL--HVTTKVVT--NFPSKKTIRLSFTSLFLGPIERLIPI 328
           G   LF KWQNI++K D+D+ L  H  TK +T  +  +K T+   F+S+F G ++ L+ +
Sbjct: 271 GLVKLFNKWQNIAYKYDKDLVLMTHFITKNITDNHGKNKTTVHGYFSSIFHGGVDSLVDL 330

Query: 329 MKTRFSELGLKRNDCIEMSWIQSVLFFADF----SIDAPLEVLMDRSSPQISDAFFTAKS 388
           M   F ELG+K+ DC E SWI + +F++      + +   E+L+DRS+ +     F+ K 
Sbjct: 331 MNKSFPELGIKKTDCKEFSWIDTTIFYSGVVNFNTANFKKEILLDRSAGK--KTAFSIKL 390

Query: 389 DYVTSPISENGLEGLWSKLLEED--KSELIFTPYGGKMSQISESQIPFPHREGRIFGIQY 448
           DYV  PI E  +  +  KL EED      +  PYGG M +ISES IPFPHR G ++ + Y
Sbjct: 391 DYVKKPIPETAMVKILEKLYEEDVGAGMYVLYPYGGIMEEISESAIPFPHRAGIMYELWY 450

Query: 449 LATWDNANENEKHLSWIREVYAYMESYVSKSPRAAYLNYRDLDLG-TNYGRNTSYEEAKV 508
            A+W+   +NEKH++W+R VY +   YVS++PR AYLNYRDLDLG TN+    +Y +A++
Sbjct: 451 TASWEKQEDNEKHINWVRSVYNFTTPYVSQNPRLAYLNYRDLDLGKTNHASPNNYTQARI 510

Query: 509 WGLKYFSDNFKRLVRVKTKVDPSNFFWNEQSIPLL 532
           WG KYF  NF RLV+VKTKVDP+NFF NEQSIP L
Sbjct: 511 WGEKYFGKNFNRLVKVKTKVDPNNFFRNEQSIPPL 540

BLAST of Cucsa.166850 vs. Swiss-Prot
Match: CASL1_CANSA (Cannabidiolic acid synthase-like 1 OS=Cannabis sativa GN=CBDAS2 PE=2 SV=1)

HSP 1 Score: 516.9 bits (1330), Expect = 2.6e-145
Identity = 254/515 (49.32%), Postives = 362/515 (70.29%), Query Frame = 1

Query: 29  QDNFLECFNSTSYSKHSIPISEVVFTNESASFSSLLRLSIRNLRFLTTTLPKPLLLVTPF 88
           Q+NFL+CF+   Y   ++  +++V+T     + S+L  +I+NLRF + T PKPL+++TP 
Sbjct: 31  QENFLKCFSQ--YIPTNVTNAKLVYTQHDQFYMSILNSTIQNLRFTSDTTPKPLVIITPL 90

Query: 89  HESHVQAAILCAREKGLQVRVRSGGHDYEGLSYISSQAPFIVIDLINLRSIKIDIKTETA 148
           + SH+Q  ILC+++ GLQ+R RSGGHD EG+SYIS Q PF+++DL N+ S+KID+ ++TA
Sbjct: 91  NVSHIQGTILCSKKVGLQIRTRSGGHDAEGMSYIS-QVPFVIVDLRNMHSVKIDVHSQTA 150

Query: 149 SVETGASLGELYYRIAKKSSIHGFPAGSCPTVGVGGHISGGGFGTLFRKYGLAADNVIDA 208
            VE GA+LGE+YY I + +    FPAG CPTVG GGH SGGG+G L R YGLAADN+IDA
Sbjct: 151 WVEAGATLGEVYYWINENNENLSFPAGYCPTVGAGGHFSGGGYGALMRNYGLAADNIIDA 210

Query: 209 KIIDFNGRIMDRSSMGEDLFWAIRGGGGASFGVILSWKLKLVSVPSIVTVFNVQRTLE-E 268
            +++ +G+++DR SMGEDLFWAIRGGGG +FG+I +WK++LV+VPS+ T+F+V++ +E  
Sbjct: 211 HLVNVDGKVLDRKSMGEDLFWAIRGGGGENFGIIAAWKIRLVAVPSMSTIFSVKKNMEIH 270

Query: 269 GATHLFQKWQNISHKLDQD--IFLHVTTKVVTN--FPSKKTIRLSFTSLFLGPIERLIPI 328
               L  KWQNI++  +++  +F H  T+ +T+    +K TI   F+S+F G ++ L+ +
Sbjct: 271 ELVKLVNKWQNIAYMYEKELLLFTHFITRNITDNQGKNKTTIHSYFSSIFHGGVDSLVDL 330

Query: 329 MKTRFSELGLKRNDCIEMSWIQSVLFFADF----SIDAPLEVLMDRSSPQISDAFFTAKS 388
           M   F ELG+K+ DC ++SWI +++F++      +     E+L+DRS  +   A F+ K 
Sbjct: 331 MNKSFPELGIKKTDCKQLSWIDTIIFYSGVVNYNTTYFKKEILLDRSGGR--KAAFSIKL 390

Query: 389 DYVTSPISENGLEGLWSKLLEED--KSELIFTPYGGKMSQISESQIPFPHREGRIFGIQY 448
           DYV  PI E  +  +  KL EED      +F PYGG M +ISES IPFPHR G ++ I Y
Sbjct: 391 DYVKKPIPETAMVTILEKLYEEDVGVGMFVFYPYGGIMDEISESAIPFPHRAGIMYEIWY 450

Query: 449 LATWDNANENEKHLSWIREVYAYMESYVSKSPRAAYLNYRDLDLG-TNYGRNTSYEEAKV 508
           +A+W+   +NEKH++WIR VY +   YVS++PR AYLNYRDLDLG TN+    +Y +A++
Sbjct: 451 IASWEKQEDNEKHINWIRNVYNFTTPYVSQNPRMAYLNYRDLDLGKTNFESPNNYTQARI 510

Query: 509 WGLKYFSDNFKRLVRVKTKVDPSNFFWNEQSIPLL 532
           WG KYF  NF RLV+VKTKVDP NFF NEQSIP L
Sbjct: 511 WGEKYFGKNFNRLVKVKTKVDPDNFFRNEQSIPPL 540

BLAST of Cucsa.166850 vs. Swiss-Prot
Match: CASL2_CANSA (Cannabidiolic acid synthase-like 2 OS=Cannabis sativa GN=CBDAS3 PE=2 SV=1)

HSP 1 Score: 514.2 bits (1323), Expect = 1.7e-144
Identity = 252/515 (48.93%), Postives = 361/515 (70.10%), Query Frame = 1

Query: 29  QDNFLECFNSTSYSKHSIPISEVVFTNESASFSSLLRLSIRNLRFLTTTLPKPLLLVTPF 88
           Q+NFL+C +   Y   ++  +++V+T     + S+L  +++NLRF + T PKPL++ TP 
Sbjct: 31  QENFLKCLSQ--YIPTNVTNAKLVYTQHDQFYMSILNSTVQNLRFTSDTTPKPLVITTPL 90

Query: 89  HESHVQAAILCAREKGLQVRVRSGGHDYEGLSYISSQAPFIVIDLINLRSIKIDIKTETA 148
           + SH+Q  ILC+++ GLQ+R RSGGHD EG+SYIS Q PF+++DL N+ S+KID+ ++TA
Sbjct: 91  NVSHIQGTILCSKKVGLQIRTRSGGHDAEGMSYIS-QVPFVIVDLRNMHSVKIDVHSQTA 150

Query: 149 SVETGASLGELYYRIAKKSSIHGFPAGSCPTVGVGGHISGGGFGTLFRKYGLAADNVIDA 208
            VE+GA+LGE+YY I + +    FPAG CPTVG GGH SGGG+G L R YGLAADN+IDA
Sbjct: 151 WVESGATLGEVYYWINENNENLSFPAGYCPTVGTGGHFSGGGYGALMRNYGLAADNIIDA 210

Query: 209 KIIDFNGRIMDRSSMGEDLFWAIRGGGGASFGVILSWKLKLVSVPSIVTVFNVQRTLE-E 268
            +++ +G+++DR SMGEDLFWAIRGGGG +FG+I +WK++LV+VPS+ T+F+V++ +E  
Sbjct: 211 HLVNVDGKVLDRKSMGEDLFWAIRGGGGENFGIIAAWKIRLVAVPSMSTIFSVKKNMEIH 270

Query: 269 GATHLFQKWQNISHKLDQD--IFLHVTTKVVTN--FPSKKTIRLSFTSLFLGPIERLIPI 328
               L  KWQNI++  +++  +F H  T+ +T+    +K TI   F+S+F G ++ L+ +
Sbjct: 271 ELVKLVNKWQNIAYMYEKELLLFTHFITRNITDNQGKNKTTIHSYFSSIFHGGVDSLVDL 330

Query: 329 MKTRFSELGLKRNDCIEMSWIQSVLFFADF----SIDAPLEVLMDRSSPQISDAFFTAKS 388
           M   F ELG+K+ DC ++SWI +++F++      + +   E+L+DRS  +   A F+ K 
Sbjct: 331 MNKSFPELGIKKTDCKQLSWIDTIIFYSGVVNYNTTNFKKEILLDRSGGR--KAAFSIKL 390

Query: 389 DYVTSPISENGLEGLWSKLLEED--KSELIFTPYGGKMSQISESQIPFPHREGRIFGIQY 448
           DYV  PI E  +  +  KL EED      +F PYGG M +ISES IPFPHR G  + I Y
Sbjct: 391 DYVKKPIPETAMVTILEKLYEEDVGVGMFVFYPYGGIMDEISESAIPFPHRAGITYEIWY 450

Query: 449 LATWDNANENEKHLSWIREVYAYMESYVSKSPRAAYLNYRDLDLG-TNYGRNTSYEEAKV 508
           +A+W+   +NEKH++WIR VY +   YVS++PR AYLNYRDLDLG TN+    +Y +A++
Sbjct: 451 IASWEKQEDNEKHINWIRNVYNFTTPYVSQNPRMAYLNYRDLDLGKTNFESPNNYTQARI 510

Query: 509 WGLKYFSDNFKRLVRVKTKVDPSNFFWNEQSIPLL 532
           WG KYF  NF RLV+VKTKVDP NFF NEQSIP L
Sbjct: 511 WGEKYFGKNFNRLVKVKTKVDPDNFFRNEQSIPPL 540

BLAST of Cucsa.166850 vs. Swiss-Prot
Match: THCAI_CANSA (Inactive tetrahydrocannabinolic acid synthase OS=Cannabis sativa PE=3 SV=1)

HSP 1 Score: 513.1 bits (1320), Expect = 3.8e-144
Identity = 256/515 (49.71%), Postives = 359/515 (69.71%), Query Frame = 1

Query: 29  QDNFLECFNSTSYSKHSIPISEVVFTNESASFSSLLRLSIRNLRFLTTTLPKPLLLVTPF 88
           Q+NFL+CF+   Y  ++    + ++T     + S+L  +I+NLRF + T PKPL++VTP 
Sbjct: 31  QENFLKCFSE--YIPNNPANPKFIYTQHDQLYMSVLNSTIQNLRFTSDTTPKPLVIVTPS 90

Query: 89  HESHVQAAILCAREKGLQVRVRSGGHDYEGLSYISSQAPFIVIDLINLRSIKIDIKTETA 148
           + SH+QA+ILC+++ GLQ+R RSGGHD EGLSYIS Q PF ++DL N+ ++K+DI ++TA
Sbjct: 91  NVSHIQASILCSKKVGLQIRTRSGGHDAEGLSYIS-QVPFAIVDLRNMHTVKVDIHSQTA 150

Query: 149 SVETGASLGELYYRIAKKSSIHGFPAGSCPTVGVGGHISGGGFGTLFRKYGLAADNVIDA 208
            VE GA+LGE+YY I + +    FP G CPTVGVGGH SGGG+G L R YGLAADN+IDA
Sbjct: 151 WVEAGATLGEVYYWINEMNENFSFPGGYCPTVGVGGHFSGGGYGALMRNYGLAADNIIDA 210

Query: 209 KIIDFNGRIMDRSSMGEDLFWAIRGGGGASFGVILSWKLKLVSVPSIVTVFNVQRTLE-E 268
            +++ +G+++DR SMGEDLFWAIRGGGG +FG+I +WK+KLV VPS  T+F+V++ +E  
Sbjct: 211 HLVNVDGKVLDRKSMGEDLFWAIRGGGGENFGIIAAWKIKLVVVPSKATIFSVKKNMEIH 270

Query: 269 GATHLFQKWQNISHKLDQDIFL--HVTTKVVT--NFPSKKTIRLSFTSLFLGPIERLIPI 328
           G   LF KWQNI++K D+D+ L  H  T+ +T  +  +K T+   F+S+FLG ++ L+ +
Sbjct: 271 GLVKLFNKWQNIAYKYDKDLMLTTHFRTRNITDNHGKNKTTVHGYFSSIFLGGVDSLVDL 330

Query: 329 MKTRFSELGLKRNDCIEMSWIQSVLFFADF----SIDAPLEVLMDRSSPQISDAFFTAKS 388
           M   F ELG+K+ DC E+SWI + +F++      + +   E+L+DRS+ +     F+ K 
Sbjct: 331 MNKSFPELGIKKTDCKELSWIDTTIFYSGVVNYNTANFKKEILLDRSAGK--KTAFSIKL 390

Query: 389 DYVTSPISENGLEGLWSKLLEED--KSELIFTPYGGKMSQISESQIPFPHREGRIFGIQY 448
           DYV   I E  +  +  KL EE+      +  PYGG M +ISES IPFPHR G ++ + Y
Sbjct: 391 DYVKKLIPETAMVKILEKLYEEEVGVGMYVLYPYGGIMDEISESAIPFPHRAGIMYELWY 450

Query: 449 LATWDNANENEKHLSWIREVYAYMESYVSKSPRAAYLNYRDLDLG-TNYGRNTSYEEAKV 508
            ATW+   +NEKH++W+R VY +   YVS++PR AYLNYRDLDLG TN     +Y +A++
Sbjct: 451 TATWEKQEDNEKHINWVRSVYNFTTPYVSQNPRLAYLNYRDLDLGKTNPESPNNYTQARI 510

Query: 509 WGLKYFSDNFKRLVRVKTKVDPSNFFWNEQSIPLL 532
           WG KYF  NF RLV+VKTK DP+NFF NEQSIP L
Sbjct: 511 WGEKYFGKNFNRLVKVKTKADPNNFFRNEQSIPPL 540

BLAST of Cucsa.166850 vs. Swiss-Prot
Match: RETOL_ARATH (Reticuline oxidase-like protein OS=Arabidopsis thaliana GN=At4g20830 PE=1 SV=2)

HSP 1 Score: 501.5 bits (1290), Expect = 1.1e-140
Identity = 251/527 (47.63%), Postives = 370/527 (70.21%), Query Frame = 1

Query: 10  FLLLCVSLIWLCGSSSDSLQDNFLECFNSTSYSKHSIPISEVVFTNESASFSSLLRLSIR 69
           F LL +SL     S S+S+ ++FL+CF+  + S  S  I++ VF+  + +FSS+LR  IR
Sbjct: 17  FFLLFLSLPLSSFSQSNSVYNSFLKCFSDKTKSPQS-QITDNVFSQTNPAFSSVLRAYIR 76

Query: 70  NLRFLTTTLPKPLLLVTPFHESHVQAAILCAREKGLQVRVRSGGHDYEGLSYISSQAPFI 129
           N RF T++  KP +++TP  ESHV AA+ C++     +++RSGGHDY+GLSYIS + PF 
Sbjct: 77  NARFNTSSTLKPTIIITPRSESHVSAAVTCSKTLNFLLKIRSGGHDYDGLSYISDK-PFF 136

Query: 130 VIDLINLRSIKIDIKTETASVETGASLGELYYRIAKKSSIHGFPAGSCPTVGVGGHISGG 189
           ++D+ N+R + +DI + +A +  GA+LGE+YYRI +KS +HGFPAG CPTVGVGGH+SGG
Sbjct: 137 ILDMSNIRDVSVDIASNSAWISAGATLGEVYYRIWEKSRVHGFPAGVCPTVGVGGHLSGG 196

Query: 190 GFGTLFRKYGLAADNVIDAKIIDFNGRIMDRSSMGEDLFWAIRGGGGASFGVILSWKLKL 249
           G+G + RK+GL+ D V DAKI+D NGR++DR +MGEDLFWAI GGGG S+GV+L +K+KL
Sbjct: 197 GYGNMVRKFGLSVDYVEDAKIVDVNGRVLDRKAMGEDLFWAITGGGGGSYGVVLGYKVKL 256

Query: 250 VSVPSIVTVFNVQRTLEEGATHLFQKWQNISHKLDQDIFLHVTTKVVTNFPSKKTIRLSF 309
           V VPS+VTVF V++ ++ GA  +  KWQ++  K D ++F+ +  + VT     KT+R S 
Sbjct: 257 VPVPSVVTVFRVEQYMDSGAVDMVHKWQSVGPKTDPNLFMRMLIQPVTR-KKVKTVRASV 316

Query: 310 TSLFLGPIERLIPIMKTRFSELGLKRNDCIEMSWIQSVLFFADFSIDAPL---EVLMDRS 369
            +LFLG  + ++ ++   F ELGLK+ +C EM+W QS L++ D  ++A     +V +DR+
Sbjct: 317 VALFLGRADEVVALLSKEFPELGLKKENCSEMTWFQSALWW-DNRLNATQVDPKVFLDRN 376

Query: 370 SPQISDAFFTAKSDYVTSPISENGLEGLWSKLLEEDKSELIFTPYGGKMSQISESQIPFP 429
               + +F   KSDYV + I + G+E L+ K++E  K  L+F PYGGKM++++ +  PFP
Sbjct: 377 LD--TSSFGKRKSDYVATAIPKKGIESLFKKMIELGKIGLVFNPYGGKMAEVAVNAKPFP 436

Query: 430 HREGRIFGIQYLATW-DNANENEK-HLSWIREVYAYMESYVSKSPRAAYLNYRDLDLGTN 489
           HR  ++F IQY   W +N+ E EK +L+  + +Y++M  +VSK+PR++Y NYRD+D+G N
Sbjct: 437 HR-NKLFKIQYSVNWKENSAEIEKGYLNQAKVLYSFMTGFVSKNPRSSYFNYRDVDIGVN 496

Query: 490 YGRNTSYEEAKVWGLKYFSDNFKRLVRVKTKVDPSNFFWNEQSIPLL 532
                SY+E +V+G KYF +NF RLV++KT VDP NFF NEQSIP L
Sbjct: 497 DHGANSYKEGEVYGRKYFGENFDRLVKIKTAVDPGNFFRNEQSIPTL 536

BLAST of Cucsa.166850 vs. TrEMBL
Match: B9I0Y4_POPTR (Uncharacterized protein OS=Populus trichocarpa GN=POPTR_0011s16190g PE=4 SV=2)

HSP 1 Score: 675.2 bits (1741), Expect = 6.4e-191
Identity = 336/529 (63.52%), Postives = 407/529 (76.94%), Query Frame = 1

Query: 4   FRRIHLFLLLCVSLIWLCGSSSDSLQDNFLECFNSTSYSKHSIPISEVVFTNESASFSSL 63
           F    L L+     +W   +SS S Q+ FL+CF+S    + S P+SEV+ T  S+ +SS+
Sbjct: 6   FNMRFLLLIFLAPSVW--PASSSSAQEKFLQCFSSRI--QPSNPVSEVILTKNSSDYSSV 65

Query: 64  LRLSIRNLRFLTTTLPKPLLLVTPFHESHVQAAILCAREKGLQVRVRSGGHDYEGLSYIS 123
           LR SIRN RFL TT+ KP  ++TPF ESH+QAAI+CARE G+Q+RVRSGGHDYEGLS+ S
Sbjct: 66  LRSSIRNYRFLNTTIVKPQFIITPFDESHIQAAIVCAREYGMQIRVRSGGHDYEGLSFES 125

Query: 124 SQAPFIVIDLINLRSIKIDIKTETASVETGASLGELYYRIAKKSSIHGFPAGSCPTVGVG 183
            Q  F+++DL  L SI +DI+ ETA +  GAS+GELYY+IA KS +HGFPAGSCPTVGVG
Sbjct: 126 YQE-FVLVDLAKLSSIIVDIENETAWIGAGASIGELYYKIADKSKVHGFPAGSCPTVGVG 185

Query: 184 GHISGGGFGTLFRKYGLAADNVIDAKIIDFNGRIMDRSSMGEDLFWAIRGGGGASFGVIL 243
           GH SGGGFGT+FRKYGLAADNVIDA+I+D NGRI+DR SMGEDLFWAIRGGG ASFGVI 
Sbjct: 186 GHFSGGGFGTIFRKYGLAADNVIDAQIVDANGRILDRESMGEDLFWAIRGGGAASFGVIF 245

Query: 244 SWKLKLVSVPSIVTVFNVQRTLEEGATHLFQKWQNISHKLDQDIFLHVTTKVVTNFPS-K 303
           SWKL+LVSVP  VTVFN++RTLE+GA++L QKWQ+I  K  +D+FLH   +V T+ P+  
Sbjct: 246 SWKLRLVSVPPTVTVFNIKRTLEQGASNLLQKWQSIGDKFHEDLFLHAAIEVATSSPNGN 305

Query: 304 KTIRLSFTSLFLGPIERLIPIMKTRFSELGLKRNDCIEMSWIQSVLFFADFSIDAPLEVL 363
           KTIR+SF SLFLG  E L+P+M+  F ELGL R +C EMSWIQS+L+F  FS    L+VL
Sbjct: 306 KTIRVSFVSLFLGRAEELLPVMQDSFPELGLMRENCSEMSWIQSILYFGGFSPRDSLDVL 365

Query: 364 MDRSSPQISDAFFTAKSDYVTSPISENGLEGLWSKLLEEDKSELIFTPYGGKMSQISESQ 423
           + R++      FF  KSDYV  PISE GLEGL+ +LLEE+ S LI TPYGG+MS+IS+S+
Sbjct: 366 LSRTAQ--FKGFFKGKSDYVKEPISETGLEGLYRRLLEEEASMLILTPYGGRMSEISDSE 425

Query: 424 IPFPHREGRIFGIQYLATWDNANENEKHLSWIREVYAYMESYVSKSPRAAYLNYRDLDLG 483
           IPFPHR G IF IQYL TWD   E E+HL W+R +YAYM  YVS SPRAAYLNYRDLDLG
Sbjct: 426 IPFPHRSGNIFKIQYLITWDVEEETERHLKWMRRLYAYMAPYVSNSPRAAYLNYRDLDLG 485

Query: 484 TNYGRNTSYEEAKVWGLKYFSDNFKRLVRVKTKVDPSNFFWNEQSIPLL 532
            N   NTS+ +A VWGLKYF +NFKRLV+VKT  DPSNFF NEQSIP+L
Sbjct: 486 RNNNGNTSFAKASVWGLKYFKNNFKRLVQVKTATDPSNFFRNEQSIPVL 527

BLAST of Cucsa.166850 vs. TrEMBL
Match: B9GGJ0_POPTR (Uncharacterized protein OS=Populus trichocarpa GN=POPTR_0001s46700g PE=4 SV=2)

HSP 1 Score: 653.7 bits (1685), Expect = 2.0e-184
Identity = 325/524 (62.02%), Postives = 404/524 (77.10%), Query Frame = 1

Query: 9   LFLLLCVSLIWLCGSSSDSLQDNFLECFNSTSYSKHSIPISEVVFTNESASFSSLLRLSI 68
           L L+L VSL     SSS    + FL+CF+S  + +HS   SEV+ T  S+++SS+L+ SI
Sbjct: 11  LLLILLVSLARSADSSSS--HEMFLQCFSS--HIQHSKSYSEVILTKNSSAYSSVLQSSI 70

Query: 69  RNLRFLTTTLPKPLLLVTPFHESHVQAAILCAREKGLQVRVRSGGHDYEGLSYISSQAPF 128
           RN RFL T+  KP  ++TPF+E  +QAAI+CA++  +Q+RVRSGGHDYEGLS++S Q  F
Sbjct: 71  RNFRFLNTSTLKPQFIITPFNEFEIQAAIVCAKKYDMQIRVRSGGHDYEGLSFLSYQE-F 130

Query: 129 IVIDLINLRSIKIDIKTETASVETGASLGELYYRIAKKSSIHGFPAGSCPTVGVGGHISG 188
           +++DL  L SI +DI+ ETA +  GAS+GELYYRIA+KS +HGFPAG+CPTVGVGGH SG
Sbjct: 131 VLVDLAELSSISVDIENETAWIGAGASIGELYYRIAEKSKVHGFPAGTCPTVGVGGHFSG 190

Query: 189 GGFGTLFRKYGLAADNVIDAKIIDFNGRIMDRSSMGEDLFWAIRGGGGASFGVILSWKLK 248
           GGFGT+FRKYGLAADN+IDA+I+D NGRI+DR SMGEDLFWAIRGGG ASFGV+ SWK++
Sbjct: 191 GGFGTIFRKYGLAADNLIDARIVDANGRILDRESMGEDLFWAIRGGGAASFGVVFSWKVR 250

Query: 249 LVSVPSIVTVFNVQRTLEEGATHLFQKWQNISHKLDQDIFLHVTTKVVTNFPS-KKTIRL 308
           LVSVP  VTVFN+ +TL++GA++L  KWQNI  KL +D+FLH T  V T+ P+  KTI++
Sbjct: 251 LVSVPPTVTVFNIGKTLQQGASNLLHKWQNIGDKLHEDLFLHATIAVATSSPNGNKTIQV 310

Query: 309 SFTSLFLGPIERLIPIMKTRFSELGLKRNDCIEMSWIQSVLFFADFSIDAPLEVLMDRSS 368
           SF SLFLG  E L+P+M+  F ELGL R +C EMSWIQSVL+F  FS    L+VL+ R++
Sbjct: 311 SFVSLFLGRAEELLPMMQDSFPELGLMRENCSEMSWIQSVLYFGGFSPSDSLDVLLSRTA 370

Query: 369 PQISDAFFTAKSDYVTSPISENGLEGLWSKLLEEDKSELIFTPYGGKMSQISESQIPFPH 428
                 FF  KSDYV  PISE GLEGL+ +LLEE+ S LI TPYGG+MS+IS+S+ PFPH
Sbjct: 371 Q--FKGFFKGKSDYVKEPISETGLEGLYKRLLEEETSMLILTPYGGRMSEISDSETPFPH 430

Query: 429 REGRIFGIQYLATWDNANENEKHLSWIREVYAYMESYVSKSPRAAYLNYRDLDLGTNYGR 488
           R G IF IQY+ TWD   E EK+L W+R++YAYM  YVS SPRAAYLNYRDLDLG N   
Sbjct: 431 RSGNIFEIQYIITWDVEEETEKNLKWMRKLYAYMAPYVSNSPRAAYLNYRDLDLGRNNYG 490

Query: 489 NTSYEEAKVWGLKYFSDNFKRLVRVKTKVDPSNFFWNEQSIPLL 532
           NTS+ +A VWGLKYF +NFKRL RVKT  DPSNFF NEQSIP+L
Sbjct: 491 NTSFAKASVWGLKYFKNNFKRLARVKTATDPSNFFRNEQSIPVL 527

BLAST of Cucsa.166850 vs. TrEMBL
Match: A0A061G780_THECC (FAD-binding Berberine family protein OS=Theobroma cacao GN=TCM_027064 PE=4 SV=1)

HSP 1 Score: 650.2 bits (1676), Expect = 2.2e-183
Identity = 327/526 (62.17%), Postives = 402/526 (76.43%), Query Frame = 1

Query: 9   LFLLLCVSLIWLCGSSSDSLQDNFLECFNSTSYSKHSIPISEVVFTNESASFSSLLRLSI 68
           LFL L VS+   C S+S+S +++FL+CF+S  +   S   S+V+F+   + + S+LR SI
Sbjct: 21  LFLFLSVSV---CFSASNSTKESFLQCFSS--HLPPSNITSDVIFSQNGSQYLSILRSSI 80

Query: 69  RNLRFLTTTLPKPLLLVTPFHESHVQAAILCAREKGLQVRVRSGGHDYEGLSYISSQAPF 128
           RNLRFL  +  KP  L+TP+ E+H+QAA++C++E GL VRVRSGGHDYEGLSYIS   PF
Sbjct: 81  RNLRFLNAS--KPQYLITPYSEAHIQAAVICSKEYGLHVRVRSGGHDYEGLSYISD-VPF 140

Query: 129 IVIDLINLRSIKIDIKTETASVETGASLGELYYRIAKKSSIHGFPAGSCPTVGVGGHISG 188
           I+IDL  +RSI +DIK E A V  GA+LGELYY IA  S++HGFPAGSCPTVGVGGH SG
Sbjct: 141 IIIDLFQMRSISLDIKNEYAWVGGGATLGELYYSIAGSSNLHGFPAGSCPTVGVGGHFSG 200

Query: 189 GGFGTLFRKYGLAADNVIDAKIIDFNGRIMDRSSMGEDLFWAIRGGGGASFGVILSWKLK 248
           GGFGT+FRKYGLAADNVIDAKI+D NG I+DR SMGEDLFWAIRGGG  SFGVI SWK+K
Sbjct: 201 GGFGTIFRKYGLAADNVIDAKIVDVNGNILDRKSMGEDLFWAIRGGGAQSFGVIFSWKIK 260

Query: 249 LVSVPSIVTVFNVQRTLEEGATHLFQKWQNISHKLDQDIFLHVTTKVVT-NFPSKKTIRL 308
           LV VP  VTVF++++TLE+GAT L  KWQ ++ KL QD+F+HV   V + N    KT+R+
Sbjct: 261 LVRVPPTVTVFSIRKTLEQGATKLLHKWQTVADKLHQDLFIHVVMGVPSANSKGNKTVRV 320

Query: 309 SFTSLFLGPIERLIPIMKTR--FSELGLKRNDCIEMSWIQSVLFFADFSIDAPLEVLMDR 368
           SF  LFLG  ERL+ +++ +  F ELG+ R++C EMSWIQSVL+FA FSI   L+VL++R
Sbjct: 321 SFDCLFLGTAERLLSLLQQQDGFPELGVTRDNCTEMSWIQSVLYFAGFSITESLDVLLNR 380

Query: 369 SSPQISDAFFTAKSDYVTSPISENGLEGLWSKLLEEDKSELIFTPYGGKMSQISESQIPF 428
           +    S  F  AKSDYV  PI E+GLEGL+  L+EE+ S LI TPYGG+MSQIS S+IPF
Sbjct: 381 T--MSSTQFSKAKSDYVKEPIPESGLEGLYKMLVEEENSILILTPYGGRMSQISSSEIPF 440

Query: 429 PHREGRIFGIQYLATWDNANENEKHLSWIREVYAYMESYVSKSPRAAYLNYRDLDLGTNY 488
           PHR G ++GIQY+  W  A E EKHL W+R +Y YME YVS SPRAAY NYRDLDLG N 
Sbjct: 441 PHRSGNLYGIQYILDWAAAEETEKHLGWMRRLYKYMEPYVSNSPRAAYFNYRDLDLGRNN 500

Query: 489 GRNTSYEEAKVWGLKYFSDNFKRLVRVKTKVDPSNFFWNEQSIPLL 532
            R+TSY +A VWG KYF++NFKRLVRVKT  DP NFFWNEQSIP+L
Sbjct: 501 KRHTSYAQAAVWGFKYFNNNFKRLVRVKTSTDPDNFFWNEQSIPVL 536

BLAST of Cucsa.166850 vs. TrEMBL
Match: B9SB00_RICCO (Reticuline oxidase, putative OS=Ricinus communis GN=RCOM_1335790 PE=4 SV=1)

HSP 1 Score: 647.9 bits (1670), Expect = 1.1e-182
Identity = 312/521 (59.88%), Postives = 414/521 (79.46%), Query Frame = 1

Query: 12  LLCVSLIWLCGSSSDSLQDNFLECFNSTSYSKHSIPISEVVFTNESASFSSLLRLSIRNL 71
           LL + L  +C ++S+S+   FL+CF+S  + ++S PIS+V+    S+ +SS+L+ SIRNL
Sbjct: 11  LLVILLALVCSATSNSVDVKFLQCFSS--HLRNSKPISQVILPRNSSRYSSVLQSSIRNL 70

Query: 72  RFLTTTLPKPLLLVTPFHESHVQAAILCAREKGLQVRVRSGGHDYEGLSYISSQAPFIVI 131
           RF +T+  KP  ++TPF+ESH+QAA++CA+   + +R RSGGHDYEGLSY+S +  F+++
Sbjct: 71  RFSSTSALKPEFIITPFNESHIQAAVVCAKAYNMLIRTRSGGHDYEGLSYVSDEK-FVLV 130

Query: 132 DLINLRSIKIDIKTETASVETGASLGELYYRIAKKSSIHGFPAGSCPTVGVGGHISGGGF 191
           DL +LRSI +DI++E+A VE+GA+LGELYY+IA+KS+++GFPAGSCPTVGVGGHISGGGF
Sbjct: 131 DLASLRSISVDIESESAWVESGATLGELYYKIAEKSNVYGFPAGSCPTVGVGGHISGGGF 190

Query: 192 GTLFRKYGLAADNVIDAKIIDFNGRIMDRSSMGEDLFWAIRGGGGASFGVILSWKLKLVS 251
           GT+FRKYGLA+D VIDAK+++ NG I+DR SMGEDLFWAIRGGGGASFG+ILSWK++LVS
Sbjct: 191 GTIFRKYGLASDAVIDAKLVNVNGEILDRESMGEDLFWAIRGGGGASFGIILSWKVRLVS 250

Query: 252 VPSIVTVFNVQRTLEEGATHLFQKWQNISHKLDQDIFLHVTTKVVTNFP-SKKTIRLSFT 311
           VP  VTVF+  RTLE+G + L  KWQ + ++L +D+FLH  T VV + P S +TI++SF 
Sbjct: 251 VPPTVTVFSAARTLEQGGSKLLHKWQTVGNQLPEDLFLHAVTGVVVSSPNSNRTIQISFD 310

Query: 312 SLFLGPIERLIPIMKTRFSELGLKRNDCIEMSWIQSVLFFADFSIDAPLEVLMDRSSPQI 371
           +L+LG  E++IP+M+++F ELG+ R +C EMSWIQSVL+FA F  +  L+VL++R +   
Sbjct: 311 ALYLGTAEQVIPLMQSKFPELGITRENCTEMSWIQSVLYFAGFPKNESLDVLLNRKTQ-- 370

Query: 372 SDAFFTAKSDYVTSPISENGLEGLWSKLLEEDKSELIFTPYGGKMSQISESQIPFPHREG 431
              F  AK+DYV  PISE GLEGL+ +LLE + S LI TPYGG+MS+ISES+IPFPHR G
Sbjct: 371 PKEFSKAKADYVQEPISETGLEGLYKRLLESETSMLILTPYGGRMSEISESEIPFPHRNG 430

Query: 432 RIFGIQYLATWDNANENEKHLSWIREVYAYMESYVSKSPRAAYLNYRDLDLGTNYGR-NT 491
            ++ IQYL TWD   E ++H+ WIR +Y+YM +YVSK PRAAYLNYRDLDLG N  + NT
Sbjct: 431 NLYKIQYLVTWDVEEETKQHIEWIRSLYSYMAAYVSKLPRAAYLNYRDLDLGRNKKKGNT 490

Query: 492 SYEEAKVWGLKYFSDNFKRLVRVKTKVDPSNFFWNEQSIPL 531
           S+ +A VWGLKYF +NFKRLV VKT +DPSNFF NEQSIPL
Sbjct: 491 SFAQASVWGLKYFKNNFKRLVNVKTAIDPSNFFRNEQSIPL 526

BLAST of Cucsa.166850 vs. TrEMBL
Match: A0A061G8G8_THECC (FAD-binding Berberine family protein OS=Theobroma cacao GN=TCM_027069 PE=4 SV=1)

HSP 1 Score: 642.9 bits (1657), Expect = 3.5e-181
Identity = 324/528 (61.36%), Postives = 401/528 (75.95%), Query Frame = 1

Query: 7   IHLFLLLCVSLIWLCGSSSDSLQDNFLECFNSTSYSKHSIPISEVVFTNESASFSSLLRL 66
           + LFL L +S+   C S+S+S +++FL+CF+S  + + S   S+V+F+  S+ + SLL+ 
Sbjct: 18  LKLFLFLSLSV---CFSASNSTKESFLQCFSS--HLRPSNITSDVIFSQNSSQYLSLLQS 77

Query: 67  SIRNLRFLTTTLPKPLLLVTPFHESHVQAAILCAREKGLQVRVRSGGHDYEGLSYISSQA 126
           SIRNLRFL  +  KP  L+TP+ E+H+QAA++C++E GL VRVRSGGHDYEGLSYIS   
Sbjct: 78  SIRNLRFLNAS--KPQYLITPYSEAHIQAAVICSKEHGLHVRVRSGGHDYEGLSYISD-V 137

Query: 127 PFIVIDLINLRSIKIDIKTETASVETGASLGELYYRIAKKSSIHGFPAGSCPTVGVGGHI 186
           PFI+IDL  +RSI +DIK E A V  GA+LGELYY IA  S++HGFPAGSCPTVGVGGH 
Sbjct: 138 PFIIIDLFQMRSISLDIKNEYAWVGGGATLGELYYSIAGSSNLHGFPAGSCPTVGVGGHF 197

Query: 187 SGGGFGTLFRKYGLAADNVIDAKIIDFNGRIMDRSSMGEDLFWAIRGGGGASFGVILSWK 246
           SGGGFGT+FRKYGLAADNVIDAKI+D NG I+D  SMGEDLFWAIRGGG  SFGVI SWK
Sbjct: 198 SGGGFGTIFRKYGLAADNVIDAKIVDVNGNILDTKSMGEDLFWAIRGGGAQSFGVIFSWK 257

Query: 247 LKLVSVPSIVTVFNVQRTLEEGATHLFQKWQNISHKLDQDIFLHVTTKVVT-NFPSKKTI 306
           +KLV VP  VTVF++++ LE+GAT L  KWQ ++ KL QD+F+HV   V + N    KT+
Sbjct: 258 IKLVRVPPTVTVFSIRKRLEQGATKLLHKWQTVADKLHQDLFIHVVMGVPSANSKGNKTV 317

Query: 307 RLSFTSLFLGPIERLIPIMK--TRFSELGLKRNDCIEMSWIQSVLFFADFSIDAPLEVLM 366
           R+SF  LFLG  ERL+ +++    F ELG+ R++C EMSWIQSVL+FA FSI   L+VL+
Sbjct: 318 RVSFDCLFLGTAERLLSLLQQPDGFPELGVTRDNCTEMSWIQSVLYFAGFSITESLDVLL 377

Query: 367 DRSSPQISDAFFTAKSDYVTSPISENGLEGLWSKLLEEDKSELIFTPYGGKMSQISESQI 426
           +R+    S  F  AKSD+V  PI E+GLEGL+  L+EE  S LI TPYGG+MSQIS S+I
Sbjct: 378 NRT--MSSTQFSKAKSDHVKEPIPESGLEGLYKMLVEEKNSILILTPYGGRMSQISSSEI 437

Query: 427 PFPHREGRIFGIQYLATWDNANENEKHLSWIREVYAYMESYVSKSPRAAYLNYRDLDLGT 486
           PFPHR G ++GIQY+  W  A E EKHL W+R +Y YME YVS SPRAAY NYRDLDLG 
Sbjct: 438 PFPHRSGNLYGIQYILDWAAAEETEKHLGWMRRLYKYMEPYVSNSPRAAYFNYRDLDLGR 497

Query: 487 NYGRNTSYEEAKVWGLKYFSDNFKRLVRVKTKVDPSNFFWNEQSIPLL 532
           N  R+TSY +A VWG KYF++NFKRLVRVKT  DP NFFWNEQSIP+L
Sbjct: 498 NNKRHTSYAQAAVWGFKYFNNNFKRLVRVKTSTDPDNFFWNEQSIPVL 535

BLAST of Cucsa.166850 vs. TAIR10
Match: AT1G30760.1 (AT1G30760.1 FAD-binding Berberine family protein)

HSP 1 Score: 543.1 bits (1398), Expect = 1.9e-154
Identity = 272/525 (51.81%), Postives = 369/525 (70.29%), Query Frame = 1

Query: 11  LLLCVSLIWLCGSSSDSLQDNFLECFNSTSYSKHSIPISEVVFTNE--SASFSSLLRLSI 70
           LLL +S I L   S  ++Q +F+ C    S +  S P+    FT++  ++SF   L  S 
Sbjct: 17  LLLSLSYIPL---SFSTIQQDFVMCLVDNSDA--SFPMDSSFFTHDLNASSFKLALETSA 76

Query: 71  RNLRFLTTTLPKPLLLVTPFHESHVQAAILCAREKGLQVRVRSGGHDYEGLSYISS-QAP 130
           +NLR+L  + PKP  +  P +E+HVQAA+LCA++  L +R+RSGGHDYEGLSY+S  +  
Sbjct: 77  QNLRYLMPSNPKPEFIFEPLYETHVQAAVLCAKKLKLHLRLRSGGHDYEGLSYVSEMETA 136

Query: 131 FIVIDLINLRSIKIDIKTETASVETGASLGELYYRIAKKSSIHGFPAGSCPTVGVGGHIS 190
           F+++DL  LR I +DI++ +A V  GAS+GE+YYRI +KS IHGFPAG C ++G+GGHI 
Sbjct: 137 FVIVDLSKLRQISVDIESNSAWVHAGASIGEVYYRIQEKSKIHGFPAGLCTSLGIGGHII 196

Query: 191 GGGFGTLFRKYGLAADNVIDAKIIDFNGRIMDRSSMGEDLFWAIRGGGGASFGVILSWKL 250
           GG +G++ RK+GL ADNV+DA+I+D +G+I++R++MGED+FWAIRGGGG SFGVIL+WK+
Sbjct: 197 GGAYGSMMRKFGLGADNVLDARIVDADGKILNRAAMGEDVFWAIRGGGGGSFGVILAWKI 256

Query: 251 KLVSVPSIVTVFNVQRTLEEGATHLFQKWQNISHKLDQDIFLHVTTKVVTNFPSKK--TI 310
           KLV VP IVTVF V RTLE+  T L  KWQ ++ KLD+D+F+ V  +  +  P  K  TI
Sbjct: 257 KLVPVPEIVTVFTVTRTLEQDGTKLLYKWQQVADKLDEDLFIRVIIQPTSKTPKSKERTI 316

Query: 311 RLSFTSLFLGPIERLIPIMKTRFSELGLKRNDCIEMSWIQSVLFFADFSIDAPLEVLMDR 370
             S+   FLG   RL+ +M+  F +LGL + DC+E SWI+SV++ A F   AP E L+D 
Sbjct: 317 STSYQGQFLGDANRLLQVMQRSFPQLGLTKKDCLETSWIKSVMYIAGFPSTAPSEALLDG 376

Query: 371 SSPQISDAFFTAKSDYVTSPISENGLEGLWSKLLEEDKSELIFTPYGGKMSQISESQIPF 430
            S  +   +F AKSDYV  PI   GLEGLW KLLEED    I+ PYGG M++I E++ PF
Sbjct: 377 KS--LFKNYFKAKSDYVEEPIPVEGLEGLWEKLLEEDSPLTIWNPYGGMMAKIPETETPF 436

Query: 431 PHREGRIFGIQYLATWDNANENE-KHLSWIREVYAYMESYVSKSPRAAYLNYRDLDLGTN 490
           PHR G +F IQ+L  W +   +E KH+ W+RE+Y+YME YVSKSPR+AY+NYRDLDLG N
Sbjct: 437 PHRSGTLFKIQWLTLWQDGKTSEAKHMGWMREMYSYMEQYVSKSPRSAYVNYRDLDLGMN 496

Query: 491 YGRNTSYEEAKVWGLKYFSDNFKRLVRVKTKVDPSNFFWNEQSIP 530
            G+ +   +A+ WG +YF  NF+RLV +K K DP NFF +EQSIP
Sbjct: 497 -GKGS---DAREWGNRYFKGNFERLVEIKAKFDPENFFRHEQSIP 530

BLAST of Cucsa.166850 vs. TAIR10
Match: AT5G44390.1 (AT5G44390.1 FAD-binding Berberine family protein)

HSP 1 Score: 534.6 bits (1376), Expect = 6.9e-152
Identity = 266/515 (51.65%), Postives = 360/515 (69.90%), Query Frame = 1

Query: 23  SSSDSLQDNFLECFNSTSYSKHSIPISEVVFTNESASFSSLLRLSIRNLRFLTTTLPKPL 82
           +SS SLQD+F++C    +  + ++  +       ++ F+ +L  + +N R+LT T+PKP 
Sbjct: 28  TSSTSLQDDFIKCLYRNTNVRFTLDKTFFTPERNASIFTEVLESTAQNQRYLTKTMPKPG 87

Query: 83  LLVTPFHESHVQAAILCAREKGLQVRVRSGGHDYEGLSYISS-QAPFIVIDLINLRSIKI 142
            +  P HESHVQA+++C+++  +  RVRSGGHDYEG+SY+S  + PF++IDL  LR I +
Sbjct: 88  FIFKPVHESHVQASVICSKKLEIHFRVRSGGHDYEGVSYVSQIEKPFVLIDLSKLRQINV 147

Query: 143 DIKTETASVETGASLGELYYRIAKKSSIHGFPAGSCPTVGVGGHISGGGFGTLFRKYGLA 202
           DIK  +A VE GA++GELYYRIA+KS  HGFPAG  P++G+GGHI+GG +G+L RKYGLA
Sbjct: 148 DIKDTSAWVEAGATVGELYYRIAEKSKFHGFPAGVYPSLGIGGHITGGAYGSLMRKYGLA 207

Query: 203 ADNVIDAKIIDFNGRIMDRSSMGEDLFWAIRGGGGASFGVILSWKLKLVSVPSIVTVFNV 262
           ADNV+DAKI+D NG+++DR+SMGEDLFWAIRGG G SFG+ILSWK+KLV VP  +TVF V
Sbjct: 208 ADNVLDAKIVDANGKLLDRASMGEDLFWAIRGGSGGSFGIILSWKIKLVPVPETLTVFTV 267

Query: 263 QRTLEEGAT-HLFQKWQNISHKLDQDIFLHVTTKVVTNFPSKKTIRLSFTSLFLGPIERL 322
            +T E+  +  +  KWQ I+  L  ++FL V   V  N  + KT+ +++   FLG    L
Sbjct: 268 TKTFEQDRSFKILSKWQEIADNLVDELFLRVFFTVSGN-KANKTVTMAYIGQFLGEKGTL 327

Query: 323 IPIMKTRFSELGLKRNDCIEMSWIQSVLFFADFSID--APLEVLMDRSSPQISDAFFTAK 382
           + +MK  F ELGL + DCIEMSWI S+++ + F  +   P+E+L+   SP I   +F  K
Sbjct: 328 MEVMKKDFPELGLTQKDCIEMSWIDSIIYNSGFPTNPPPPIEILLQAKSP-IGKVYFKGK 387

Query: 383 SDYVTSPISENGLEGLWSKLLEEDKSELIFTPYGGKMSQISESQIPFPHREGRIFGIQYL 442
           SD+   PI   GLEG++ KLLEED + +I+TPYGGKM +I ES+IPFPHR G  F IQY 
Sbjct: 388 SDFAKKPIPVLGLEGMFKKLLEEDAALVIWTPYGGKMDKIPESEIPFPHRNGTNFMIQYY 447

Query: 443 ATW-DNANENEKHLSWIREVYAYMESYVSKSPRAAYLNYRDLDLGTNYGRNTS-YEEAKV 502
            +W D+     +   WIRE+Y YM  YVS +PR AY+NYRDLDLG N   + S + EAK+
Sbjct: 448 RSWSDSEKRPNRRTKWIRELYGYMTPYVSSNPRQAYVNYRDLDLGQNKDNSKSNFIEAKI 507

Query: 503 WGLKYFSDNFKRLVRVKTKVDPSNFFWNEQSIPLL 532
           WG  YF DNF RLVR+K+KVDP NFF +EQSIP L
Sbjct: 508 WGANYFKDNFNRLVRIKSKVDPDNFFRHEQSIPTL 540

BLAST of Cucsa.166850 vs. TAIR10
Match: AT2G34790.1 (AT2G34790.1 FAD-binding Berberine family protein)

HSP 1 Score: 534.6 bits (1376), Expect = 6.9e-152
Identity = 261/525 (49.71%), Postives = 363/525 (69.14%), Query Frame = 1

Query: 11  LLLCVSLIWLCGSSSDSLQDNFLECFNSTSYSKHSIPISEVVFTNESAS--FSSLLRLSI 70
           L L   L+     SS +LQ +F++C      S  S PI+   F+ +  +  F   L  + 
Sbjct: 12  LFLVTLLLISVPLSSSTLQQDFVKCL--VDNSDVSFPITASFFSPDQNATLFKEELESTA 71

Query: 71  RNLRFLTTTLPKPLLLVTPFHESHVQAAILCAREKGLQVRVRSGGHDYEGLSYISS-QAP 130
           +NLR+LT + PKP+ +  P +E+HVQAA++CA++  L +R+RSGGHDYEGLS+++  + P
Sbjct: 72  QNLRYLTPSNPKPVFIFEPLYETHVQAAVVCAKKLQLHLRLRSGGHDYEGLSFVAEDETP 131

Query: 131 FIVIDLINLRSIKIDIKTETASVETGASLGELYYRIAKKSSIHGFPAGSCPTVGVGGHIS 190
           F+++DL  LR + +D+ + +A    GA++GE+YYRI +KS  HGFPAG C ++G+GGH+ 
Sbjct: 132 FVIVDLSKLRQVDVDLDSNSAWAHAGATIGEVYYRIQEKSQTHGFPAGLCSSLGIGGHLV 191

Query: 191 GGGFGTLFRKYGLAADNVIDAKIIDFNGRIMDRSSMGEDLFWAIRGGGGASFGVILSWKL 250
           GG +G++ RK+GL ADNV+DA+I+D NG+I+DR++MGED+FWAIRGGGG SFGVIL+WK+
Sbjct: 192 GGAYGSMMRKFGLGADNVLDARIVDANGQILDRAAMGEDVFWAIRGGGGGSFGVILAWKI 251

Query: 251 KLVSVPSIVTVFNVQRTLEEGATHLFQKWQNISHKLDQDIFLHVTTKVV--TNFPSKKTI 310
           KLV VP+ VTVF V +TLE+  T +  KW+ I+ KLD D+F+ V       T  P  +TI
Sbjct: 252 KLVPVPATVTVFTVTKTLEQDGTKVLYKWEQIADKLDDDLFIRVIISPASKTTKPGNRTI 311

Query: 311 RLSFTSLFLGPIERLIPIMKTRFSELGLKRNDCIEMSWIQSVLFFADFSIDAPLEVLMDR 370
            +S+ + FLG   RL+ +M+  F ELGL + DC EMSWI+SV++ A F   A  E L+  
Sbjct: 312 SMSYQAQFLGDSNRLLQVMQKSFPELGLTKKDCTEMSWIKSVMYIAGFPNSAAPEALL-- 371

Query: 371 SSPQISDAFFTAKSDYVTSPISENGLEGLWSKLLEEDKSELIFTPYGGKMSQISESQIPF 430
           +   +    F AKSD+V  PI   GLEGLW + LEED    I+ PYGG MS+ISES+IPF
Sbjct: 372 AGKSLFKNHFKAKSDFVKEPIPVEGLEGLWERFLEEDSPLTIWNPYGGMMSRISESEIPF 431

Query: 431 PHREGRIFGIQYLATW-DNANENEKHLSWIREVYAYMESYVSKSPRAAYLNYRDLDLGTN 490
           PHR G +F IQ+L+TW D     E+H+ WIRE+Y+YME YVSK+PR AY+NYRDLDLGTN
Sbjct: 432 PHRNGTLFKIQWLSTWQDGKVSEERHMKWIREMYSYMEQYVSKNPRQAYVNYRDLDLGTN 491

Query: 491 YGRNTSYEEAKVWGLKYFSDNFKRLVRVKTKVDPSNFFWNEQSIP 530
            G      +A+ WG KY+  NF+RLV++K + DP NFF +EQS+P
Sbjct: 492 EGET----DAREWGAKYYKGNFERLVKIKGEFDPDNFFRHEQSVP 528

BLAST of Cucsa.166850 vs. TAIR10
Match: AT1G30700.1 (AT1G30700.1 FAD-binding Berberine family protein)

HSP 1 Score: 530.4 bits (1365), Expect = 1.3e-150
Identity = 259/527 (49.15%), Postives = 363/527 (68.88%), Query Frame = 1

Query: 9   LFLLLCVSLIWLCGSSSDSLQDNFLECFNSTSYSKHSIPISEVVFTNESASFSSLLRLSI 68
           + +L  V  IW   SSS +  + F +C  S S  KH  PIS  +F + + S+SS+L+ +I
Sbjct: 6   ILVLFFVVFIWQSSSSSAN-SETFTQCLTSNSDPKH--PISPAIFFSGNGSYSSVLQANI 65

Query: 69  RNLRFLTTTLPKPLLLVTPFHESHVQAAILCAREKGLQVRVRSGGHDYEGLSYIS-SQAP 128
           RNLRF TT+ PKP L++   HESHVQAAI C +   LQ+++RSGGHDY+GLSY++ S  P
Sbjct: 66  RNLRFNTTSTPKPFLIIAATHESHVQAAITCGKRHNLQMKIRSGGHDYDGLSYVTYSGKP 125

Query: 129 FIVIDLINLRSIKIDIKTETASVETGASLGELYYRIAKKSSIHGFPAGSCPTVGVGGHIS 188
           F V+D+ NLRS+ +D+ ++TA V+TGA LGE+YY I +KS    +PAG CPTVGVGGHIS
Sbjct: 126 FFVLDMFNLRSVDVDVASKTAWVQTGAILGEVYYYIWEKSKTLAYPAGICPTVGVGGHIS 185

Query: 189 GGGFGTLFRKYGLAADNVIDAKIIDFNGRIMDRSSMGEDLFWAIRGGGGASFGVILSWKL 248
           GGG+G + RKYGL  DN IDA+++D NG+I+DR  MGEDL+WAI GGGG S+GV+L++K+
Sbjct: 186 GGGYGNMMRKYGLTVDNTIDARMVDVNGKILDRKLMGEDLYWAINGGGGGSYGVVLAYKI 245

Query: 249 KLVSVPSIVTVFNVQRTLEEGATHLFQKWQNISHKLDQDIFLHVTTKVVT-NFPSKKTIR 308
            LV VP  VTVF + RTLE+ AT +  +WQ ++ KL  ++F+     VV     S+KT+R
Sbjct: 246 NLVEVPENVTVFRISRTLEQNATDIIHRWQQVAPKLPDELFIRTVIDVVNGTVSSQKTVR 305

Query: 309 LSFTSLFLGPIERLIPIMKTRFSELGLKRNDCIEMSWIQSVLFFADFSIDAPLEVLMDRS 368
            +F ++FLG    L+ I+  RF ELGL R+DC E SWIQSVLF+ +  + +   +L+ R+
Sbjct: 306 TTFIAMFLGDTTTLLSILNRRFPELGLVRSDCTETSWIQSVLFWTNIQVGSSETLLLQRN 365

Query: 369 SPQISDAFFTAKSDYVTSPISENGLEGLWSKLLEEDKSELIFTPYGGKMSQISESQIPFP 428
            P     +   KSDYV  PIS  GLE +W K++E +   + F PYGG+M +IS +  PFP
Sbjct: 366 QPV---NYLKRKSDYVREPISRTGLESIWKKMIELEIPTMAFNPYGGEMGRISSTVTPFP 425

Query: 429 HREGRIFGIQYLATWDNANENEKHLSWIREVYAYMESYVSKSPRAAYLNYRDLDLGTN-- 488
           +R G ++ IQY A W +    ++++   R++Y +M  +VSK+PR ++ NYRD+DLG N  
Sbjct: 426 YRAGNLWKIQYGANWRDETLTDRYMELTRKLYQFMTPFVSKNPRQSFFNYRDVDLGINSH 485

Query: 489 YGRNTSYEEAKVWGLKYFSDNFKRLVRVKTKVDPSNFFWNEQSIPLL 532
            G+ +SY E K +G KYF+ NF+RLV++KT+VD  NFF NEQSIP+L
Sbjct: 486 NGKISSYVEGKRYGKKYFAGNFERLVKIKTRVDSGNFFRNEQSIPVL 526

BLAST of Cucsa.166850 vs. TAIR10
Match: AT5G44360.1 (AT5G44360.1 FAD-binding Berberine family protein)

HSP 1 Score: 530.4 bits (1365), Expect = 1.3e-150
Identity = 272/536 (50.75%), Postives = 370/536 (69.03%), Query Frame = 1

Query: 7   IHLFLLLCVSLIWLCGSSSDSLQDNFLECFNSTSYSKHSIPISEV------VFTNESASF 66
           + LFL+  V  +    SSS S +D FL C ++ S S    P S +      V+T+ S S 
Sbjct: 9   LSLFLVFLVKWVNSDSSSSPS-KDQFLSCMSTHSDSSFINPKSFIHKPDSRVYTDFSQSL 68

Query: 67  SSLLRLSIRNLRFLTT--TLPKPLLLVTPFHESHVQAAILCAREKGLQVRVRSGGHDYEG 126
            S      +N RFLT   T  KP+L+VTP  ++ +Q ++LC+R+ G++VR +SGGHDYEG
Sbjct: 69  IS------QNYRFLTLNFTSQKPILIVTPRTDTEIQRSLLCSRKLGVKVRTKSGGHDYEG 128

Query: 127 LSYISSQAPFIVIDLINLRSIKIDIKTETASVETGASLGELYYRIAKKSSIHGFPAGSCP 186
           LSY+S  +PFI++DL+N+RSI+I++  ETA V  GA++GELYY+IAK S IHGFPAG+CP
Sbjct: 129 LSYLSLHSPFIILDLVNVRSIEINLADETAWVGAGATIGELYYKIAKSSKIHGFPAGTCP 188

Query: 187 TVGVGGHISGGGFGTLFRKYGLAADNVIDAKIIDFNGRIMD-RSSMGEDLFWAIRGGGGA 246
           +VGVGGH SGGGFG + RK+GLAADNV+DA+ +D NGRI + R  MGEDLFWAIRGGG A
Sbjct: 189 SVGVGGHFSGGGFGAMMRKHGLAADNVVDARFVDANGRIYNSRREMGEDLFWAIRGGGAA 248

Query: 247 SFGVILSWKLKLVSVPSIVTVFNVQRTLEEGATHLFQKWQNISHKLDQDIFLHVTTKVVT 306
           SFGV+LSWK+KLV VP  VT F     L +  T +  +WQ I+ +LD ++F+ V   +  
Sbjct: 249 SFGVVLSWKVKLVRVPEKVTCFRRNLPLTQNMTKIVHRWQQIAAELDDNLFIRVIVSI-- 308

Query: 307 NFPSKKTIRLSFTSLFLGPIERLIPIMKTRFSELGLKRNDCIEMSWIQSVLFFADFSIDA 366
              S  +++ +F + +LG I++LIP+M  +F ELGL   DC EM+WI S+++F ++    
Sbjct: 309 ---SGGSVQTTFQANYLGGIDKLIPLMNQKFPELGLTFQDCSEMTWIDSIMYF-NWKKGQ 368

Query: 367 PLEVLMDRSSPQISDAFFTAKSDYVTSPISENGLEGLWSKLLEEDKSELIFTPYGGKMSQ 426
           PLE L+DR   + +D +F AKSD+V +PI E GLEG+W++  E +   +I  P GGKM +
Sbjct: 369 PLETLLDRGQ-RYNDLYFKAKSDFVKNPIPEIGLEGIWTRFHEVESPIMIMEPLGGKMYE 428

Query: 427 ISESQIPFPHREGRIFGIQYLATW--DNANENEKHLSWIREVYAYMESYVSKSPRAAYLN 486
           I E++ PFPHR G ++ IQY+  W   +    EKH++W+R +Y YM  YVS SPR AYLN
Sbjct: 429 IGETETPFPHRRGNLYNIQYMVKWRLKDIGVMEKHVTWMRLLYRYMRVYVSASPRGAYLN 488

Query: 487 YRDLDLGTNYGRNTSYEEAKVWGLKYFSDNFKRLVRVKTKVDPSNFFWNEQSIPLL 532
           YRDLDLG N G NTS+E+AK+WG +YF  NFKRL  VK K+DP+NFF NEQS+P L
Sbjct: 489 YRDLDLGMNRGVNTSFEDAKLWGFRYFGSNFKRLAIVKGKIDPTNFFRNEQSVPPL 530

BLAST of Cucsa.166850 vs. NCBI nr
Match: gi|449435884|ref|XP_004135724.1| (PREDICTED: tetrahydrocannabinolic acid synthase-like [Cucumis sativus])

HSP 1 Score: 1089.7 bits (2817), Expect = 0.0e+00
Identity = 542/542 (100.00%), Postives = 542/542 (100.00%), Query Frame = 1

Query: 1   MICFRRIHLFLLLCVSLIWLCGSSSDSLQDNFLECFNSTSYSKHSIPISEVVFTNESASF 60
           MICFRRIHLFLLLCVSLIWLCGSSSDSLQDNFLECFNSTSYSKHSIPISEVVFTNESASF
Sbjct: 1   MICFRRIHLFLLLCVSLIWLCGSSSDSLQDNFLECFNSTSYSKHSIPISEVVFTNESASF 60

Query: 61  SSLLRLSIRNLRFLTTTLPKPLLLVTPFHESHVQAAILCAREKGLQVRVRSGGHDYEGLS 120
           SSLLRLSIRNLRFLTTTLPKPLLLVTPFHESHVQAAILCAREKGLQVRVRSGGHDYEGLS
Sbjct: 61  SSLLRLSIRNLRFLTTTLPKPLLLVTPFHESHVQAAILCAREKGLQVRVRSGGHDYEGLS 120

Query: 121 YISSQAPFIVIDLINLRSIKIDIKTETASVETGASLGELYYRIAKKSSIHGFPAGSCPTV 180
           YISSQAPFIVIDLINLRSIKIDIKTETASVETGASLGELYYRIAKKSSIHGFPAGSCPTV
Sbjct: 121 YISSQAPFIVIDLINLRSIKIDIKTETASVETGASLGELYYRIAKKSSIHGFPAGSCPTV 180

Query: 181 GVGGHISGGGFGTLFRKYGLAADNVIDAKIIDFNGRIMDRSSMGEDLFWAIRGGGGASFG 240
           GVGGHISGGGFGTLFRKYGLAADNVIDAKIIDFNGRIMDRSSMGEDLFWAIRGGGGASFG
Sbjct: 181 GVGGHISGGGFGTLFRKYGLAADNVIDAKIIDFNGRIMDRSSMGEDLFWAIRGGGGASFG 240

Query: 241 VILSWKLKLVSVPSIVTVFNVQRTLEEGATHLFQKWQNISHKLDQDIFLHVTTKVVTNFP 300
           VILSWKLKLVSVPSIVTVFNVQRTLEEGATHLFQKWQNISHKLDQDIFLHVTTKVVTNFP
Sbjct: 241 VILSWKLKLVSVPSIVTVFNVQRTLEEGATHLFQKWQNISHKLDQDIFLHVTTKVVTNFP 300

Query: 301 SKKTIRLSFTSLFLGPIERLIPIMKTRFSELGLKRNDCIEMSWIQSVLFFADFSIDAPLE 360
           SKKTIRLSFTSLFLGPIERLIPIMKTRFSELGLKRNDCIEMSWIQSVLFFADFSIDAPLE
Sbjct: 301 SKKTIRLSFTSLFLGPIERLIPIMKTRFSELGLKRNDCIEMSWIQSVLFFADFSIDAPLE 360

Query: 361 VLMDRSSPQISDAFFTAKSDYVTSPISENGLEGLWSKLLEEDKSELIFTPYGGKMSQISE 420
           VLMDRSSPQISDAFFTAKSDYVTSPISENGLEGLWSKLLEEDKSELIFTPYGGKMSQISE
Sbjct: 361 VLMDRSSPQISDAFFTAKSDYVTSPISENGLEGLWSKLLEEDKSELIFTPYGGKMSQISE 420

Query: 421 SQIPFPHREGRIFGIQYLATWDNANENEKHLSWIREVYAYMESYVSKSPRAAYLNYRDLD 480
           SQIPFPHREGRIFGIQYLATWDNANENEKHLSWIREVYAYMESYVSKSPRAAYLNYRDLD
Sbjct: 421 SQIPFPHREGRIFGIQYLATWDNANENEKHLSWIREVYAYMESYVSKSPRAAYLNYRDLD 480

Query: 481 LGTNYGRNTSYEEAKVWGLKYFSDNFKRLVRVKTKVDPSNFFWNEQSIPLLYHYEDDTKV 540
           LGTNYGRNTSYEEAKVWGLKYFSDNFKRLVRVKTKVDPSNFFWNEQSIPLLYHYEDDTKV
Sbjct: 481 LGTNYGRNTSYEEAKVWGLKYFSDNFKRLVRVKTKVDPSNFFWNEQSIPLLYHYEDDTKV 540

Query: 541 TK 543
           TK
Sbjct: 541 TK 542

BLAST of Cucsa.166850 vs. NCBI nr
Match: gi|659068675|ref|XP_008445632.1| (PREDICTED: tetrahydrocannabinolic acid synthase-like [Cucumis melo])

HSP 1 Score: 1029.6 bits (2661), Expect = 1.9e-297
Identity = 509/542 (93.91%), Postives = 524/542 (96.68%), Query Frame = 1

Query: 1   MICFRRIHLFLLLCVSLIWLCGSSSDSLQDNFLECFNSTSYSKHSIPISEVVFTNESASF 60
           MIC RR HLFLL CVSLIWLC SSSDSLQDNFLECFNSTSYSKHSIP+SEVVFTNESASF
Sbjct: 3   MICLRRTHLFLLFCVSLIWLC-SSSDSLQDNFLECFNSTSYSKHSIPVSEVVFTNESASF 62

Query: 61  SSLLRLSIRNLRFLTTTLPKPLLLVTPFHESHVQAAILCAREKGLQVRVRSGGHDYEGLS 120
           SSL RLSIRNLRFLTTTLPKPL LVTPFHESHVQAAI+CAREKGLQVRVRSGGHDYEGLS
Sbjct: 63  SSLFRLSIRNLRFLTTTLPKPLFLVTPFHESHVQAAIVCAREKGLQVRVRSGGHDYEGLS 122

Query: 121 YISSQAPFIVIDLINLRSIKIDIKTETASVETGASLGELYYRIAKKSSIHGFPAGSCPTV 180
           Y+SSQAPFIVIDLINLRSI IDI+ ETASV TGA+LGELYYRIAKKSSIHGFPAGSCPTV
Sbjct: 123 YVSSQAPFIVIDLINLRSITIDIEDETASVGTGAALGELYYRIAKKSSIHGFPAGSCPTV 182

Query: 181 GVGGHISGGGFGTLFRKYGLAADNVIDAKIIDFNGRIMDRSSMGEDLFWAIRGGGGASFG 240
           GVGGHISGGGFGTLFRKYGLAADNVIDAKIIDFNGRIMDRSSMGEDLFWAIRGGGGASFG
Sbjct: 183 GVGGHISGGGFGTLFRKYGLAADNVIDAKIIDFNGRIMDRSSMGEDLFWAIRGGGGASFG 242

Query: 241 VILSWKLKLVSVPSIVTVFNVQRTLEEGATHLFQKWQNISHKLDQDIFLHVTTKVVTNFP 300
           VILSWKLKLVSVPSIVTVFNVQRTLE+GATHLFQKWQNISHKLDQDIFLHVTTKVVT+FP
Sbjct: 243 VILSWKLKLVSVPSIVTVFNVQRTLEQGATHLFQKWQNISHKLDQDIFLHVTTKVVTDFP 302

Query: 301 SKKTIRLSFTSLFLGPIERLIPIMKTRFSELGLKRNDCIEMSWIQSVLFFADFSIDAPLE 360
           SKKTIRLSFTSLFLGPIERLIPIMKTRFSELGLKR+DCIEMSWIQSVLFFADFSID PLE
Sbjct: 303 SKKTIRLSFTSLFLGPIERLIPIMKTRFSELGLKRSDCIEMSWIQSVLFFADFSIDTPLE 362

Query: 361 VLMDRSSPQISDAFFTAKSDYVTSPISENGLEGLWSKLLEEDKSELIFTPYGGKMSQISE 420
           VLM+RSSPQISDAFFTAKSDYV SPIS+ GLEGLW KLLEE+KSELI TPYGGKMSQISE
Sbjct: 363 VLMERSSPQISDAFFTAKSDYVISPISQKGLEGLWKKLLEEEKSELILTPYGGKMSQISE 422

Query: 421 SQIPFPHREGRIFGIQYLATWDNANENEKHLSWIREVYAYMESYVSKSPRAAYLNYRDLD 480
           SQ PFPHREGRIFGIQYLATWDNANE EKHLSWIREVYAYM+ YVSKSPRAAYLNYRDLD
Sbjct: 423 SQTPFPHREGRIFGIQYLATWDNANETEKHLSWIREVYAYMKPYVSKSPRAAYLNYRDLD 482

Query: 481 LGTNYGRNTSYEEAKVWGLKYFSDNFKRLVRVKTKVDPSNFFWNEQSIPLLYHYEDDTKV 540
           LG NYGRNTSYEEAKVWGLKYFSDNF+RLVRVKTKVDPSNFFWNEQSIPLLYHYEDDT++
Sbjct: 483 LGRNYGRNTSYEEAKVWGLKYFSDNFERLVRVKTKVDPSNFFWNEQSIPLLYHYEDDTRI 542

Query: 541 TK 543
           TK
Sbjct: 543 TK 543

BLAST of Cucsa.166850 vs. NCBI nr
Match: gi|449435886|ref|XP_004135725.1| (PREDICTED: tetrahydrocannabinolic acid synthase-like [Cucumis sativus])

HSP 1 Score: 775.4 bits (2001), Expect = 6.5e-221
Identity = 392/541 (72.46%), Postives = 449/541 (82.99%), Query Frame = 1

Query: 3   CFRRIHLFLLLCVSLIWLCGSSSDSLQDNFLECFNSTSY--SKHSIPISEVVFTNESASF 62
           C  R +LF L  +S I L  SS     D+F+ECFNSTSY  S  SIPIS+VVFTN+SA F
Sbjct: 6   CRIRHYLFPLFYLSFICLSSSSHSLQADSFVECFNSTSYYYSPQSIPISKVVFTNKSAIF 65

Query: 63  SSLLRLSIRNLRFLTTTLPKPLLLVTPFHESHVQAAILCAREKGLQVRVRSGGHDYEGLS 122
           SSLL+ SI+NLRF  T+LPKPL LVTPFH+SHVQAAI+CA +KG Q+R+RSGGHDYEG+S
Sbjct: 66  SSLLQSSIKNLRFFNTSLPKPLFLVTPFHQSHVQAAIVCANKKGFQIRLRSGGHDYEGIS 125

Query: 123 YISS-QAPFIVIDLINLRSIKIDIKTETASVETGASLGELYYRIAKKSSIHGFPAGSCPT 182
           Y+SS Q+ FI++DL N RSI ID+KTETA+VE GA+LGELYYRIA+KS  HGFPAG+CPT
Sbjct: 126 YVSSDQSQFILLDLSNYRSIDIDMKTETATVEAGATLGELYYRIAEKSPTHGFPAGTCPT 185

Query: 183 VGVGGHISGGGFGTLFRKYGLAADNVIDAKIIDFNGRIMDRSSMGEDLFWAIRGGGGASF 242
           VG+GGH+SGGGFGTLFRKYGLAADNVIDAKI+DFNGRIMDR+SMGEDLFWAIRGGGGASF
Sbjct: 186 VGMGGHVSGGGFGTLFRKYGLAADNVIDAKIVDFNGRIMDRNSMGEDLFWAIRGGGGASF 245

Query: 243 GVILSWKLKLVSVPSIVTVFNVQRTLEEGATHLFQKWQNISHKLDQDIFLHVTTKVV--- 302
           GVILSWKLKLV VPS VTVF VQ+TLE+GA HLFQKWQ I+HKL +D+FLHVT  V+   
Sbjct: 246 GVILSWKLKLVYVPSNVTVFLVQKTLEQGAIHLFQKWQTIAHKLHEDLFLHVTIGVIDEQ 305

Query: 303 --TNFPSKKTIRLSFTSLFLGPIERLIPIMKTRFSELGLKRNDCIEMSWIQSVLFFADFS 362
             T   S KTI +SF SLFLGP+ERLIP+M + F ELGL+RN+C EMSWIQSVL+FA  S
Sbjct: 306 NKTPNMSSKTILISFVSLFLGPVERLIPLMNSHFPELGLERNNCTEMSWIQSVLYFAGIS 365

Query: 363 IDAPLEVLMDRSSPQISDA-FFTAKSDYVTSPISENGLEGLWSKLLEEDKSELIFTPYGG 422
           I+AP E+L+ R  P IS+  FF AKSD+V SPI + GLEGLW+K+LEE  S LI +PYGG
Sbjct: 366 IEAPPEILLKR--PPISNVLFFKAKSDFVISPIPQIGLEGLWTKMLEEPASFLILSPYGG 425

Query: 423 KMSQISESQIPFPHREGRIFGIQYLATWDNANENEKHLSWIREVYAYMESYVSKSPRAAY 482
           KM QIS+ + PFPHR+G  FGIQYL TW+NANE  +HLSWIREVY YME YVSK PRAAY
Sbjct: 426 KMRQISDLETPFPHRKGNTFGIQYLVTWENANETYRHLSWIREVYDYMEPYVSKYPRAAY 485

Query: 483 LNYRDLDLGTNYGRNTSYEEAKVWGLKYFSDNFKRLVRVKTKVDPSNFFWNEQSIPL-LY 534
           LNYRDLDLG N GRNTSYEEAKVWGLKYF +NF RLVRVKTKVDP NFFWNEQSIP  +Y
Sbjct: 486 LNYRDLDLGRNCGRNTSYEEAKVWGLKYFKNNFDRLVRVKTKVDPLNFFWNEQSIPTSIY 544

BLAST of Cucsa.166850 vs. NCBI nr
Match: gi|659068889|ref|XP_008446963.1| (PREDICTED: tetrahydrocannabinolic acid synthase-like [Cucumis melo])

HSP 1 Score: 767.3 bits (1980), Expect = 1.8e-218
Identity = 389/541 (71.90%), Postives = 444/541 (82.07%), Query Frame = 1

Query: 3   CFRRIHLFLLLCVSLIWLCGSSSDSLQDNFLECFNSTSY--SKHSIPISEVVFTNESAS- 62
           C  R +LFLL  +  IWL  SS     ++FLECFNSTSY  S  SIPIS+VVFT++S++ 
Sbjct: 6   CRIRHYLFLLFYLPFIWLSSSSYSLQAESFLECFNSTSYYYSPQSIPISKVVFTSKSSAL 65

Query: 63  FSSLLRLSIRNLRFLTTTLPKPLLLVTPFHESHVQAAILCAREKGLQVRVRSGGHDYEGL 122
           FSSLL+ SI+NLRFL T+L KPL LVTPFH+SHVQ+AI+CA +KGLQ+RVRSGGHDYEG+
Sbjct: 66  FSSLLQSSIKNLRFLNTSLQKPLFLVTPFHQSHVQSAIVCANKKGLQIRVRSGGHDYEGI 125

Query: 123 SYISS-QAPFIVIDLINLRSIKIDIKTETASVETGASLGELYYRIAKKSSIHGFPAGSCP 182
           SY+SS Q+ FI++D IN RSI ID+KTETA VE GA+LGELYYRIA+KS IHGFPAGSCP
Sbjct: 126 SYVSSDQSQFILLDFINFRSIDIDMKTETAIVEAGATLGELYYRIAEKSPIHGFPAGSCP 185

Query: 183 TVGVGGHISGGGFGTLFRKYGLAADNVIDAKIIDFNGRIMDRSSMGEDLFWAIRGGGGAS 242
           TVG+GGH+ GGGFGTLFRKYGLAADNVIDAKI+D NGRIMDR+SMGEDLFWAIRGGGGAS
Sbjct: 186 TVGMGGHVGGGGFGTLFRKYGLAADNVIDAKIVDVNGRIMDRNSMGEDLFWAIRGGGGAS 245

Query: 243 FGVILSWKLKLVSVPSIVTVFNVQRTLEEGATHLFQKWQNISHKLDQDIFLHVTTKVV-- 302
           FGVILSWKLKLV VPS VTVF VQ+TLE+GA HLFQKWQ I+HKL +D+FLHVT  V+  
Sbjct: 246 FGVILSWKLKLVYVPSNVTVFVVQKTLEQGAIHLFQKWQRIAHKLHEDLFLHVTMGVIDE 305

Query: 303 ---TNFPSKKTIRLSFTSLFLGPIERLIPIMKTRFSELGLKRNDCIEMSWIQSVLFFADF 362
              T   S KTI +SF SLFLG IERLIP+M + F ELGL+RN+C EMSWIQSVLFFA  
Sbjct: 306 QNKTPNMSSKTILISFVSLFLGRIERLIPLMNSHFPELGLERNNCTEMSWIQSVLFFAGI 365

Query: 363 SIDAPLEVLMDRSSPQISDAFFTAKSDYVTSPISENGLEGLWSKLLEEDKSELIFTPYGG 422
           SI+ P +VL+ R  P     FF AKSD+V SPI + G EGLW+K+LEE+ S LI TPYGG
Sbjct: 366 SIETPPKVLLKR-PPTSKMLFFKAKSDFVISPIPQIGFEGLWTKMLEEEASYLILTPYGG 425

Query: 423 KMSQISESQIPFPHREGRIFGIQYLATWDNANENEKHLSWIREVYAYMESYVSKSPRAAY 482
           KM QIS+   PFPHR+G IFGIQY+ TW+NANE  +HLSWIREVY YME YVSK PRAAY
Sbjct: 426 KMRQISDFDTPFPHRKGNIFGIQYMVTWENANETYRHLSWIREVYDYMEPYVSKHPRAAY 485

Query: 483 LNYRDLDLGTNYGRNTSYEEAKVWGLKYFSDNFKRLVRVKTKVDPSNFFWNEQSIPL-LY 534
           LNYRDLDLG N GRNTSYEEAKVWGLKY+  NF RLVRVKTKVDP NFFWNEQSIP  +Y
Sbjct: 486 LNYRDLDLGRNCGRNTSYEEAKVWGLKYYKSNFDRLVRVKTKVDPLNFFWNEQSIPTSVY 545

BLAST of Cucsa.166850 vs. NCBI nr
Match: gi|566195780|ref|XP_002317088.2| (hypothetical protein POPTR_0011s16190g [Populus trichocarpa])

HSP 1 Score: 675.2 bits (1741), Expect = 9.2e-191
Identity = 336/529 (63.52%), Postives = 407/529 (76.94%), Query Frame = 1

Query: 4   FRRIHLFLLLCVSLIWLCGSSSDSLQDNFLECFNSTSYSKHSIPISEVVFTNESASFSSL 63
           F    L L+     +W   +SS S Q+ FL+CF+S    + S P+SEV+ T  S+ +SS+
Sbjct: 6   FNMRFLLLIFLAPSVW--PASSSSAQEKFLQCFSSRI--QPSNPVSEVILTKNSSDYSSV 65

Query: 64  LRLSIRNLRFLTTTLPKPLLLVTPFHESHVQAAILCAREKGLQVRVRSGGHDYEGLSYIS 123
           LR SIRN RFL TT+ KP  ++TPF ESH+QAAI+CARE G+Q+RVRSGGHDYEGLS+ S
Sbjct: 66  LRSSIRNYRFLNTTIVKPQFIITPFDESHIQAAIVCAREYGMQIRVRSGGHDYEGLSFES 125

Query: 124 SQAPFIVIDLINLRSIKIDIKTETASVETGASLGELYYRIAKKSSIHGFPAGSCPTVGVG 183
            Q  F+++DL  L SI +DI+ ETA +  GAS+GELYY+IA KS +HGFPAGSCPTVGVG
Sbjct: 126 YQE-FVLVDLAKLSSIIVDIENETAWIGAGASIGELYYKIADKSKVHGFPAGSCPTVGVG 185

Query: 184 GHISGGGFGTLFRKYGLAADNVIDAKIIDFNGRIMDRSSMGEDLFWAIRGGGGASFGVIL 243
           GH SGGGFGT+FRKYGLAADNVIDA+I+D NGRI+DR SMGEDLFWAIRGGG ASFGVI 
Sbjct: 186 GHFSGGGFGTIFRKYGLAADNVIDAQIVDANGRILDRESMGEDLFWAIRGGGAASFGVIF 245

Query: 244 SWKLKLVSVPSIVTVFNVQRTLEEGATHLFQKWQNISHKLDQDIFLHVTTKVVTNFPS-K 303
           SWKL+LVSVP  VTVFN++RTLE+GA++L QKWQ+I  K  +D+FLH   +V T+ P+  
Sbjct: 246 SWKLRLVSVPPTVTVFNIKRTLEQGASNLLQKWQSIGDKFHEDLFLHAAIEVATSSPNGN 305

Query: 304 KTIRLSFTSLFLGPIERLIPIMKTRFSELGLKRNDCIEMSWIQSVLFFADFSIDAPLEVL 363
           KTIR+SF SLFLG  E L+P+M+  F ELGL R +C EMSWIQS+L+F  FS    L+VL
Sbjct: 306 KTIRVSFVSLFLGRAEELLPVMQDSFPELGLMRENCSEMSWIQSILYFGGFSPRDSLDVL 365

Query: 364 MDRSSPQISDAFFTAKSDYVTSPISENGLEGLWSKLLEEDKSELIFTPYGGKMSQISESQ 423
           + R++      FF  KSDYV  PISE GLEGL+ +LLEE+ S LI TPYGG+MS+IS+S+
Sbjct: 366 LSRTAQ--FKGFFKGKSDYVKEPISETGLEGLYRRLLEEEASMLILTPYGGRMSEISDSE 425

Query: 424 IPFPHREGRIFGIQYLATWDNANENEKHLSWIREVYAYMESYVSKSPRAAYLNYRDLDLG 483
           IPFPHR G IF IQYL TWD   E E+HL W+R +YAYM  YVS SPRAAYLNYRDLDLG
Sbjct: 426 IPFPHRSGNIFKIQYLITWDVEEETERHLKWMRRLYAYMAPYVSNSPRAAYLNYRDLDLG 485

Query: 484 TNYGRNTSYEEAKVWGLKYFSDNFKRLVRVKTKVDPSNFFWNEQSIPLL 532
            N   NTS+ +A VWGLKYF +NFKRLV+VKT  DPSNFF NEQSIP+L
Sbjct: 486 RNNNGNTSFAKASVWGLKYFKNNFKRLVQVKTATDPSNFFRNEQSIPVL 527