Cucsa.164940 (gene) Cucumber (Gy14) v1

BLAST of Cucsa.164940 vs. Swiss-Prot
Match: CUCM1_CUCME (Cucumisin OS=Cucumis melo PE=1 SV=1)

HSP 1 Score: 915.6 bits (2365), Expect = 3.3e-265
Identity = 455/699 (65.09%), Postives = 548/699 (78.40%), Query Frame = 1

Query: 1   MGNKLEDSASTPSHHMRMLEEVVGSSFAPEALLHSYKRSFNGFVVKLTEEEAQKISAKEN 60
           MG KLED  S   HH  MLE+VVGS+FAPE++LH+YKRSFNGF VKLTEEEA+KI++ E 
Sbjct: 38  MGRKLEDPDSAHLHHRAMLEQVVGSTFAPESVLHTYKRSFNGFAVKLTEEEAEKIASMEG 97

Query: 61  VVSVFPNEKKHLHTTRSWDFMGFTQKAPRVKQVESNIVVGVLDSGIWPESPSFSDVGYGP 120
           VVSVF NE   LHTTRSWDF+GF    PR  QVESNIVVGVLD+GIWPESPSF D G+ P
Sbjct: 98  VVSVFLNEMNELHTTRSWDFLGFPLTVPRRSQVESNIVVGVLDTGIWPESPSFDDEGFSP 157

Query: 121 PPPKWKGACQTSANFHCNRKIIGARAYRSDKFFPPEDIKSPRDSDGHGTHTASTVAGGLV 180
           PPPKWKG C+TS NF CNRKIIGAR+Y   +   P D+  PRD++GHGTHTAST AGGLV
Sbjct: 158 PPPKWKGTCETSNNFRCNRKIIGARSYHIGRPISPGDVNGPRDTNGHGTHTASTAAGGLV 217

Query: 181 NQASLYGLALGTARGGVPSARIAVYKICWSDGCYDADILAAFDDAIADGVDIISLSVGGS 240
           +QA+LYGL LGTARGGVP ARIA YK+CW+DGC D DILAA+DDAIADGVDIISLSVGG+
Sbjct: 218 SQANLYGLGLGTARGGVPLARIAAYKVCWNDGCSDTDILAAYDDAIADGVDIISLSVGGA 277

Query: 241 KPKYYFNDSIAIGAFHSMKHGILTSNSAGNDGPDYFTIRNFSPWSLSVAASSIDRKLVSR 300
            P++YF D+IAIG+FH+++ GILTSNSAGN GP++FT  + SPW LSVAAS++DRK V++
Sbjct: 278 NPRHYFVDAIAIGSFHAVERGILTSNSAGNGGPNFFTTASLSPWLLSVAASTMDRKFVTQ 337

Query: 301 VQLGNKNTFQGYTINTFDLKGKQHPLIYAGSAPNISAGFTGSSSRFCSRNSVDRNLVKGK 360
           VQ+GN  +FQG +INTFD   + +PL+     PN   GF  S+SRFC+  SV+ NL+KGK
Sbjct: 338 VQIGNGQSFQGVSINTFD--NQYYPLVSGRDIPN--TGFDKSTSRFCTDKSVNPNLLKGK 397

Query: 361 IVLCDSVLSPATFV-SLNGAVGVVMNDLGVKDNARSYPLPSSYLDPVDGDNIKTYMDRTR 420
           IV+C++   P  F  SL+GA GV+M     +D A SYPLPSS LDP D      Y+   R
Sbjct: 398 IVVCEASFGPHEFFKSLDGAAGVLMTS-NTRDYADSYPLPSSVLDPNDLLATLRYIYSIR 457

Query: 421 FPTATILKSNAVNDTSAPWIVSFSSRGPNPETYDILKPDLTAPGVEILAAWSPIATVSSG 480
            P ATI KS  + + SAP +VSFSSRGPN  T D++KPD++ PGVEILAAW  +A V  G
Sbjct: 458 SPGATIFKSTTILNASAPVVVSFSSRGPNRATKDVIKPDISGPGVEILAAWPSVAPV-GG 517

Query: 481 VRDSRTTLYNIISGTSMSCPHATAAAVYVKTFHPTWSPAAIKSALMTTATPLNAKLNTQV 540
           +R  R TL+NIISGTSMSCPH T  A YVKT++PTWSPAAIKSALMTTA+P+NA+ N Q 
Sbjct: 518 IR--RNTLFNIISGTSMSCPHITGIATYVKTYNPTWSPAAIKSALMTTASPMNARFNPQA 577

Query: 541 EFAYGAGHINPLRAVHPGLLYDAYESDYVRFLCGQGYTTAMVRRLSGDNSVCTRANSGRV 600
           EFAYG+GH+NPL+AV PGL+YDA ESDYV+FLCGQGY T  VRR++GD S CT  N+GRV
Sbjct: 578 EFAYGSGHVNPLKAVRPGLVYDANESDYVKFLCGQGYNTQAVRRITGDYSACTSGNTGRV 637

Query: 601 WDLNYPSFALSSTSSQSFNQFFRRTVTNVGSKVSTYRAKVVGVPRGLSITVNPPVLSFNA 660
           WDLNYPSF LS + SQ+FNQ+F RT+T+V  + STYRA ++  P+GL+I+VNP VLSFN 
Sbjct: 638 WDLNYPSFGLSVSPSQTFNQYFNRTLTSVAPQASTYRA-MISAPQGLTISVNPNVLSFNG 697

Query: 661 IGQKKSFTLTIRGSISQSIVSASLVWSDGHHNVRSPITV 699
           +G +KSFTLT+RGSI   +VSASLVWSDG H VRSPIT+
Sbjct: 698 LGDRKSFTLTVRGSIKGFVVSASLVWSDGVHYVRSPITI 727

BLAST of Cucsa.164940 vs. Swiss-Prot
Match: SBT43_ARATH (Subtilisin-like protease SBT4.3 OS=Arabidopsis thaliana GN=SBT4.3 PE=3 SV=1)

HSP 1 Score: 679.9 bits (1753), Expect = 3.0e-194
Identity = 351/705 (49.79%), Postives = 469/705 (66.52%), Query Frame = 1

Query: 1   MGNKLEDSASTPSHHMRMLEEVVGSSFAPEALLHSYKRSFNGFVVKLTEEEAQKISAKEN 60
           MG   E   S PSHH+ +L+++VG+  A   L+ SYKRSFNGF   L++ E+QK+   + 
Sbjct: 37  MGTLPEIKYSPPSHHLSILQKLVGTIAASHLLVRSYKRSFNGFAANLSQAESQKLQNMKE 96

Query: 61  VVSVFPNEKKHLHTTRSWDFMGFTQKAPRVKQVESNIVVGVLDSGIWPESPSFSDVGYGP 120
           VVSVFP++   L TTRSWDF+GF +KA R    ES+++VGV+DSGIWPES SF D G+GP
Sbjct: 97  VVSVFPSKSHELTTTRSWDFVGFGEKARRESVKESDVIVGVIDSGIWPESESFDDEGFGP 156

Query: 121 PPPKWKGACQTSANFHCNRKIIGARAYRSDKFFPPEDIKSPRDSDGHGTHTASTVAGGLV 180
           PP KWKG+C+    F CN K+IGAR Y  +KF       S RD +GHGTHTAST AG  V
Sbjct: 157 PPKKWKGSCKGGLKFACNNKLIGARFY--NKF-----ADSARDEEGHGTHTASTAAGNAV 216

Query: 181 NQASLYGLALGTARGGVPSARIAVYKICWSDGCYDADILAAFDDAIADGVDIISLSVGGS 240
             AS YGLA GTARGGVPSARIA YK+C+ + C D DILAAFDDAIADGVD+IS+S+   
Sbjct: 217 QAASFYGLAQGTARGGVPSARIAAYKVCF-NRCNDVDILAAFDDAIADGVDVISISISAD 276

Query: 241 KPKYYFNDSIAIGAFHSMKHGILTSNSAGNDGPDYFTIRNFSPWSLSVAASSIDRKLVSR 300
                 N S+AIG+FH+M  GI+T+ SAGN+GPD  ++ N SPW ++VAAS  DR+ + R
Sbjct: 277 YVSNLLNASVAIGSFHAMMRGIITAGSAGNNGPDQGSVANVSPWMITVAASGTDRQFIDR 336

Query: 301 VQLGNKNTFQGYTINTFDLKGKQHPLIYAGSAPNISAGFTGSSSRFCSRNSVDRNLVKGK 360
           V LGN     G ++NTF+L G + P++Y     N+S   + + + +CS   VD  LVKGK
Sbjct: 337 VVLGNGKALTGISVNTFNLNGTKFPIVY---GQNVSRNCSQAQAGYCSSGCVDSELVKGK 396

Query: 361 IVLCDSVLSPATFVSLNGAVGVVMNDLGVKDNARSYPLPSSYLDPVDGDNIKTYMDRTRF 420
           IVLCD  L       L GA+GV++ +  + D+A   P P+S L   D  +IK+Y++    
Sbjct: 397 IVLCDDFLGYRE-AYLAGAIGVIVQNTLLPDSAFVVPFPASSLGFEDYKSIKSYIESAEP 456

Query: 421 PTATILKSNAVNDTSAPWIVSFSSRGPNPETYDILKPDLTAPGVEILAAWSPIATVSS-- 480
           P A IL++  + D  AP++ SFSSRGP+    ++LKPD++APG+EILAA+SP+A+ SS  
Sbjct: 457 PQAEILRTEEIVDREAPYVPSFSSRGPSFVIQNLLKPDVSAPGLEILAAFSPVASPSSFL 516

Query: 481 GVRDSRTTLYNIISGTSMSCPHATAAAVYVKTFHPTWSPAAIKSALMTTATPLNAKLNTQ 540
              D R+  Y+++SGTSM+CPH    A YVK+FHP WSP+AIKSA+MTTATP+N K N +
Sbjct: 517 NPEDKRSVRYSVMSGTSMACPHVAGVAAYVKSFHPDWSPSAIKSAIMTTATPMNLKKNPE 576

Query: 541 VEFAYGAGHINPLRAVHPGLLYDAYESDYVRFLCGQGYTTAMVRRLSGDNSVCTRANSGR 600
            EFAYG+G INP +A  PGL+Y+    DY++ LC +G+ +  +   SG N  C+      
Sbjct: 577 QEFAYGSGQINPTKASDPGLVYEVETEDYLKMLCAEGFDSTTLTTTSGQNVTCSERT--E 636

Query: 601 VWDLNYPSFALSSTSSQSFNQFFRRTVTNVGSKVSTYRAKVVGVPRGLSITVNPPVLSFN 660
           V DLNYP+     +S   FN  F+RTVTNVG   STY+A VV +   L I++ P +L F 
Sbjct: 637 VKDLNYPTMTTFVSSLDPFNVTFKRTVTNVGFPNSTYKASVVPLQPELQISIEPEILRFG 696

Query: 661 AIGQKKSFTLTIRGSISQ--SIVSASLVWSDGHHNVRSPITVFVV 702
            + +KKSF +TI G   +  S VS+S+VWSDG H+VRSPI  + +
Sbjct: 697 FLEEKKSFVVTISGKELKDGSFVSSSVVWSDGSHSVRSPIVAYSI 727

BLAST of Cucsa.164940 vs. Swiss-Prot
Match: SBT46_ARATH (Subtilisin-like protease SBT4.6 OS=Arabidopsis thaliana GN=SBT4.6 PE=3 SV=1)

HSP 1 Score: 644.0 bits (1660), Expect = 1.9e-183
Identity = 340/697 (48.78%), Postives = 461/697 (66.14%), Query Frame = 1

Query: 13  SHHMRMLEEVVGSSFAPEALLHSYKRSFNGFVVKLTEEEAQKISAKENVVSVFPNEKKHL 72
           SHH  +L++V G S   + L+ +YKRSFNGF  +LTE E + +++ + VVSVFP++  +L
Sbjct: 51  SHHTSILQDVTGESSIQDRLVRNYKRSFNGFAARLTESEREILASMDEVVSVFPSKNLNL 110

Query: 73  HTTRSWDFMGFTQ--KAPRVKQVESNIVVGVLDSGIWPESPSFSDVGYGPPPPKWKGACQ 132
            TT SW+FMG  +  +  R   +ES+ ++GV+DSGI+PES SFS  G+GPPP KWKG C+
Sbjct: 111 QTTTSWNFMGLKEGKRTKRNPLIESDTIIGVIDSGIYPESDSFSGKGFGPPPKKWKGVCK 170

Query: 133 TSANFHCNRKIIGARAYRSDKFFPPEDIKSPRDSDGHGTHTASTVAGGLVNQASLYGLAL 192
              NF CN K+IGAR Y       PE   S RD+ GHG+HTAS  AG  V   S YGL  
Sbjct: 171 GGTNFTCNNKLIGARYYTPKLEGFPE---SARDNTGHGSHTASIAAGNAVKHVSFYGLGN 230

Query: 193 GTARGGVPSARIAVYKICWSD--GCYDADILAAFDDAIADGVDIISLSVGGSKPKYYFND 252
           GT RGGVP+ARIAVYK+C      C    ILAAFDDAIAD VDII++S+G      +  D
Sbjct: 231 GTVRGGVPAARIAVYKVCDPGVIRCTSDGILAAFDDAIADKVDIITVSLGADAVGTFEED 290

Query: 253 SIAIGAFHSMKHGILTSNSAGNDGPDYFTIRNFSPWSLSVAASSIDRKLVSRVQLGNKNT 312
           ++AIGAFH+M  GILT N AGN+GP+  TI + +PW  +VAAS+++R  +++V LGN  T
Sbjct: 291 TLAIGAFHAMAKGILTVNGAGNNGPERRTIVSMAPWLFTVAASNMNRAFITKVVLGNGKT 350

Query: 313 FQGYTINTFDLKGKQHPLIYAGSAPNISAGFTGSSSRFCSRNSVDRNLVKGKIVLCDSVL 372
             G ++N+FDL GK++PL+Y  SA   S+    SS+ FCS   +D   VKGKIVLCD+  
Sbjct: 351 IVGRSVNSFDLNGKKYPLVYGKSA---SSRCDASSAGFCSPGCLDSKRVKGKIVLCDTQR 410

Query: 373 SPATFVSLNGAVGVVMNDLGVKDNARSYPLPSSYLDPVDGDNIKTYMDRTRFPTATILKS 432
           +P    ++     +V N    +D A  +  P S L   D + + +Y++ T+ P A +LKS
Sbjct: 411 NPGEAQAMGAVASIVRNP--YEDAASVFSFPVSVLSEDDYNIVLSYVNSTKNPKAAVLKS 470

Query: 433 NAVNDTSAPWIVSFSSRGPNPETYDILKPDLTAPGVEILAAWSPIATVSSGVRDSRTTLY 492
             + +  AP + S+SSRGPNP  +DILKPD+TAPG EILAA+SP    S    D+R   Y
Sbjct: 471 ETIFNQKAPVVASYSSRGPNPLIHDILKPDITAPGSEILAAYSPYVPPSES--DTRHVKY 530

Query: 493 NIISGTSMSCPHATAAAVYVKTFHPTWSPAAIKSALMTTATPLNAKL---NTQVEFAYGA 552
            +ISGTSMSCPH    A Y+KTFHP WSP+ I+SA+MTTA P+NA     N   EFAYGA
Sbjct: 531 TVISGTSMSCPHVAGVAAYIKTFHPLWSPSMIQSAIMTTAWPMNASTSPSNELAEFAYGA 590

Query: 553 GHINPLRAVHPGLLYDAYESDYVRFLCGQGYTTAMVRRLSGDNSVCTRANSGRVW-DLNY 612
           GH++P+ A+HPGL+Y+A +SD++ FLCG  YT   +R +SGD+S CT+  +  +  +LNY
Sbjct: 591 GHVDPIAAIHPGLVYEANKSDHITFLCGFNYTGKKLRLISGDSSSCTKEQTKSLTRNLNY 650

Query: 613 PSFALSSTSSQSFNQFFRRTVTNVGSKVSTYRAKVVGVPRGLSITVNPPVLSFNAIGQKK 672
           PS +   + ++ F   FRRTVTNVG   +TY+AKVVG    L + V P VLS  ++ +KK
Sbjct: 651 PSMSAQVSGTKPFKVTFRRTVTNVGRPNATYKAKVVG--SKLKVKVVPAVLSLKSLYEKK 710

Query: 673 SFTLTIRGS--ISQSIVSASLVWSDGHHNVRSPITVF 700
           SFT+T+ G+   ++++VSA L+WSDG H VRSPI V+
Sbjct: 711 SFTVTVSGAGPKAENLVSAQLIWSDGVHFVRSPIVVY 735

BLAST of Cucsa.164940 vs. Swiss-Prot
Match: SBT44_ARATH (Subtilisin-like protease SBT4.4 OS=Arabidopsis thaliana GN=SBT4.4 PE=2 SV=1)

HSP 1 Score: 637.1 bits (1642), Expect = 2.3e-181
Identity = 331/697 (47.49%), Postives = 460/697 (66.00%), Query Frame = 1

Query: 13  SHHMRMLEEVVGSSFAPEALLHSYKRSFNGFVVKLTEEEAQKISAKENVVSVFPNEKKHL 72
           S HM +L+E+ G S     L+ SYK+SFNGF  +LTE E ++++  E VVSVFP+ K  L
Sbjct: 52  SDHMSILQEITGESLIENRLVRSYKKSFNGFAARLTESERKRLAGMERVVSVFPSRKLKL 111

Query: 73  HTTRSWDFMGFTQ--KAPRVKQVESNIVVGVLDSGIWPESPSFSDVGYGPPPPKWKGACQ 132
            TT SW+FMG  +  K  R + +ES+ ++GV+DSGI+PES SFSD G+GPPP KWKG C 
Sbjct: 112 QTTSSWNFMGLKEGIKTKRTRSIESDTIIGVIDSGIYPESDSFSDQGFGPPPKKWKGTCA 171

Query: 133 TSANFHCNRKIIGARAYRSDKFFPPEDIKSPRDSDGHGTHTASTVAGGLVNQASLYGLAL 192
              NF CN K+IGAR Y +      +  ++ RD  GHGTHTAS  AG  V  ++ YGL  
Sbjct: 172 GGKNFTCNNKVIGARDYTAKS----KANQTARDYSGHGTHTASIAAGNAVANSNFYGLGN 231

Query: 193 GTARGGVPSARIAVYKICWSDGCYDADILAAFDDAIADGVDIISLSVGGSKPKYYFNDSI 252
           GTARGGVP+ARIAVYK+C ++GC    +++AFDDAIADGVD+IS+S+       +  D I
Sbjct: 232 GTARGGVPAARIAVYKVCDNEGCDGEAMMSAFDDAIADGVDVISISIVLDNIPPFEEDPI 291

Query: 253 AIGAFHSMKHGILTSNSAGNDGPDYFTIRNFSPWSLSVAASSIDRKLVSRVQLGNKNTFQ 312
           AIGAFH+M  G+LT N+AGN+GP   T+ + +PW  SVAAS  +R  +++V LG+     
Sbjct: 292 AIGAFHAMAVGVLTVNAAGNNGPKISTVTSTAPWVFSVAASVTNRAFMAKVVLGDGKILI 351

Query: 313 GYTINTFDLKGKQHPLIYAGSAPNISAGFTGSSSRFCSRNSVDRNLVKGKIVLCDSVLSP 372
           G ++NT+D+ G  +PL+Y  SA   +       +R C    +D  LVKGKIVLCDS    
Sbjct: 352 GRSVNTYDMNGTNYPLVYGKSAALSTCSV--DKARLCEPKCLDGKLVKGKIVLCDSTKGL 411

Query: 373 ATFVSLNGAVGVVMNDLGVKDNARSYPLPSSYLDPVDGDNIKTYMDRTRFPTATILKSNA 432
                L     +V N    +   RS+P+  S+L   D  ++ +YM+ T+ P AT+LKS  
Sbjct: 412 IEAQKLGAVGSIVKNPEPDRAFIRSFPV--SFLSNDDYKSLVSYMNSTKNPKATVLKSEE 471

Query: 433 VNDTSAPWIVSFSSRGPNPETYDILKPDLTAPGVEILAAWSPIATVSSGVRDSRTTLYNI 492
           +++  AP + SFSSRGP+    DILKPD+TAPGVEILAA+SP ++ +    D+R   Y++
Sbjct: 472 ISNQRAPLVASFSSRGPSSIVSDILKPDITAPGVEILAAYSPDSSPTESEFDTRRVKYSV 531

Query: 493 ISGTSMSCPHATAAAVYVKTFHPTWSPAAIKSALMTTATPLNAKLN--TQVEFAYGAGHI 552
           +SGTSM+CPH    A YVKTFHP WSP+ I+SA+MTTA P+NA  +     EFAYG+GH+
Sbjct: 532 LSGTSMACPHVAGVAAYVKTFHPQWSPSMIQSAIMTTAWPMNASGSGFVSTEFAYGSGHV 591

Query: 553 NPLRAVHPGLLYDAYESDYVRFLCGQGYTTAMVRRLSGDNSVCTRANSGRV-WDLNYPSF 612
           +P+ A++PGL+Y+  ++D++ FLCG  YT+  +R +SGDNS CT+  S  +  +LNYP+ 
Sbjct: 592 DPIDAINPGLVYELTKADHINFLCGLNYTSDHLRIISGDNSTCTKEISKTLPRNLNYPTM 651

Query: 613 ALSSTSSQSFNQFFRRTVTNVGSKVSTYRAKVVGVPRG-LSITVNPPVLSFNAIGQKKSF 672
           +   + ++ FN  F+RTVTNVG + STY AKVV  P   LSI V+P VLS  ++ +K+SF
Sbjct: 652 SAKVSGTKPFNITFQRTVTNVGMQKSTYNAKVVKFPGSKLSIKVSPRVLSMKSMNEKQSF 711

Query: 673 TLTIRGSI--SQSIVSASLVWSDGHHNVRSPITVFVV 702
            +T+      ++  VSA+L+WSDG HNVRSPI V+ +
Sbjct: 712 MVTVSSDSIGTKQPVSANLIWSDGTHNVRSPIIVYAM 740

BLAST of Cucsa.164940 vs. Swiss-Prot
Match: SBT4D_ARATH (Subtilisin-like protease SBT4.13 OS=Arabidopsis thaliana GN=SBT4.13 PE=2 SV=1)

HSP 1 Score: 636.3 bits (1640), Expect = 3.9e-181
Identity = 342/708 (48.31%), Postives = 458/708 (64.69%), Query Frame = 1

Query: 1   MGNKLEDSASTP-SHHMRMLEEVVGSSFAPEALLHSYKRSFNGFVVKLTEEEAQKISAKE 60
           MG+    +  TP S HM +L+EV G S     L+ SYKRSFNGF  +LTE E ++++   
Sbjct: 36  MGSLSSRADYTPTSDHMNILQEVTGESSIEGRLVRSYKRSFNGFAARLTESERERVAKMV 95

Query: 61  NVVSVFPNEKKHLHTTRSWDFMGFTQ--KAPRVKQVESNIVVGVLDSGIWPESPSFSDVG 120
            VVSVFPN+K  L TT SWDFMG  +  K  R   VES+ ++GV+DSGI PES SFSD G
Sbjct: 96  GVVSVFPNKKLQLQTTTSWDFMGLKEGIKTKRNPTVESDTIIGVIDSGITPESQSFSDKG 155

Query: 121 YGPPPPKWKGACQTSANFHCNRKIIGARAYRSDKFFPPEDIKSPRDSDGHGTHTASTVAG 180
           +GPPP KWKG C    NF CN K+IGAR Y S+           RD DGHGTHTAST AG
Sbjct: 156 FGPPPQKWKGVCSGGKNFTCNNKLIGARDYTSE---------GTRDMDGHGTHTASTAAG 215

Query: 181 GLVNQASLYGLALGTARGGVPSARIAVYKICWSDGCYDADILAAFDDAIADGVDIISLSV 240
             V  AS +G+  GT RGGVP++R+A YK+C   GC    +L+AFDDAIADGVD+I++S+
Sbjct: 216 NAVVDASFFGIGNGTVRGGVPASRVAAYKVCTPTGCSSEALLSAFDDAIADGVDLITISI 275

Query: 241 GGSKPKYYFNDSIAIGAFHSMKHGILTSNSAGNDGPDYFTIRNFSPWSLSVAASSIDRKL 300
           G      + ND IAIGAFH+M  G+LT NSAGN GP   ++   +PW L+VAAS+ +R  
Sbjct: 276 GDKTASMFQNDPIAIGAFHAMAKGVLTVNSAGNSGPKPISVSGVAPWILTVAASTTNRGF 335

Query: 301 VSRVQLGNKNTFQGYTINTFDLKGKQHPLIYAGSAPNISAGFTGSSSRFCSRNSVDRNLV 360
           V++V LGN  T  G ++N +++KGK +PL+Y  SA   S+     S+  C  + VD++ V
Sbjct: 336 VTKVVLGNGKTLVGKSVNAYEMKGKDYPLVYGKSA--ASSACDAESAGLCELSCVDKSRV 395

Query: 361 KGKIVLCDSVLSPATFVSLNGAVGVVMNDLGVKDNARSYPLPSSYLDPVDGDNIKTYMDR 420
           KGKI++C         V   GAVG++       D A  +PLP++ L   D +++ +Y++ 
Sbjct: 396 KGKILVCGGP-GGLKIVESVGAVGLIYR-TPKPDVAFIHPLPAAGLLTEDFESLVSYLES 455

Query: 421 TRFPTATILKSNAVNDTSAPWIVSFSSRGPNPETYDILKPDLTAPGVEILAAWSPIATVS 480
           T  P A +LK+ A+ + ++P I SFSSRGPN    DILKPD+TAPGVEILAA+SP    S
Sbjct: 456 TDSPQAIVLKTEAIFNRTSPVIASFSSRGPNTIAVDILKPDITAPGVEILAAYSPAGEPS 515

Query: 481 SGVRDSRTTLYNIISGTSMSCPHATAAAVYVKTFHPTWSPAAIKSALMTTATPLNAKLN- 540
               D+R   Y+++SGTSMSCPH    A YVKTF+P WSP+ I+SA+MTTA P+NA    
Sbjct: 516 QD--DTRHVKYSVLSGTSMSCPHVAGVAAYVKTFNPKWSPSMIQSAIMTTAWPVNATGTG 575

Query: 541 -TQVEFAYGAGHINPLRAVHPGLLYDAYESDYVRFLCGQGYTTAMVRRLSGDNSVCTRAN 600
               EFAYG+GH++P+ A +PGL+Y+  +SD++ FLCG  YT+ +++ +SG+   C+ A 
Sbjct: 576 IASTEFAYGSGHVDPIAASNPGLVYELDKSDHIAFLCGMNYTSQVLKVISGETVTCSEAK 635

Query: 601 SGRVWDLNYPSF-ALSSTSSQSFNQFFRRTVTNVGSKVSTYRAKVV-GVPRGLSITVNPP 660
                +LNYPS  A  S S  +F   F RT+TNVG+  STY +KVV G    L + + P 
Sbjct: 636 KILPRNLNYPSMSAKLSGSGTTFTVTFNRTLTNVGTPNSTYTSKVVAGHGSKLDVKITPS 695

Query: 661 VLSFNAIGQKKSFTLTIRGSISQSIV--SASLVWSDGHHNVRSPITVF 700
           VLSF  + +K+SFT+T+ GS   S V  SA+L+WSDG HNVRSPI V+
Sbjct: 696 VLSFKTVNEKQSFTVTVTGSNLDSEVPSSANLIWSDGTHNVRSPIVVY 728

BLAST of Cucsa.164940 vs. TrEMBL
Match: A0A0A0KLR4_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_5G187880 PE=4 SV=1)

HSP 1 Score: 1419.1 bits (3672), Expect = 0.0e+00
Identity = 704/704 (100.00%), Postives = 704/704 (100.00%), Query Frame = 1

Query: 1   MGNKLEDSASTPSHHMRMLEEVVGSSFAPEALLHSYKRSFNGFVVKLTEEEAQKISAKEN 60
           MGNKLEDSASTPSHHMRMLEEVVGSSFAPEALLHSYKRSFNGFVVKLTEEEAQKISAKEN
Sbjct: 1   MGNKLEDSASTPSHHMRMLEEVVGSSFAPEALLHSYKRSFNGFVVKLTEEEAQKISAKEN 60

Query: 61  VVSVFPNEKKHLHTTRSWDFMGFTQKAPRVKQVESNIVVGVLDSGIWPESPSFSDVGYGP 120
           VVSVFPNEKKHLHTTRSWDFMGFTQKAPRVKQVESNIVVGVLDSGIWPESPSFSDVGYGP
Sbjct: 61  VVSVFPNEKKHLHTTRSWDFMGFTQKAPRVKQVESNIVVGVLDSGIWPESPSFSDVGYGP 120

Query: 121 PPPKWKGACQTSANFHCNRKIIGARAYRSDKFFPPEDIKSPRDSDGHGTHTASTVAGGLV 180
           PPPKWKGACQTSANFHCNRKIIGARAYRSDKFFPPEDIKSPRDSDGHGTHTASTVAGGLV
Sbjct: 121 PPPKWKGACQTSANFHCNRKIIGARAYRSDKFFPPEDIKSPRDSDGHGTHTASTVAGGLV 180

Query: 181 NQASLYGLALGTARGGVPSARIAVYKICWSDGCYDADILAAFDDAIADGVDIISLSVGGS 240
           NQASLYGLALGTARGGVPSARIAVYKICWSDGCYDADILAAFDDAIADGVDIISLSVGGS
Sbjct: 181 NQASLYGLALGTARGGVPSARIAVYKICWSDGCYDADILAAFDDAIADGVDIISLSVGGS 240

Query: 241 KPKYYFNDSIAIGAFHSMKHGILTSNSAGNDGPDYFTIRNFSPWSLSVAASSIDRKLVSR 300
           KPKYYFNDSIAIGAFHSMKHGILTSNSAGNDGPDYFTIRNFSPWSLSVAASSIDRKLVSR
Sbjct: 241 KPKYYFNDSIAIGAFHSMKHGILTSNSAGNDGPDYFTIRNFSPWSLSVAASSIDRKLVSR 300

Query: 301 VQLGNKNTFQGYTINTFDLKGKQHPLIYAGSAPNISAGFTGSSSRFCSRNSVDRNLVKGK 360
           VQLGNKNTFQGYTINTFDLKGKQHPLIYAGSAPNISAGFTGSSSRFCSRNSVDRNLVKGK
Sbjct: 301 VQLGNKNTFQGYTINTFDLKGKQHPLIYAGSAPNISAGFTGSSSRFCSRNSVDRNLVKGK 360

Query: 361 IVLCDSVLSPATFVSLNGAVGVVMNDLGVKDNARSYPLPSSYLDPVDGDNIKTYMDRTRF 420
           IVLCDSVLSPATFVSLNGAVGVVMNDLGVKDNARSYPLPSSYLDPVDGDNIKTYMDRTRF
Sbjct: 361 IVLCDSVLSPATFVSLNGAVGVVMNDLGVKDNARSYPLPSSYLDPVDGDNIKTYMDRTRF 420

Query: 421 PTATILKSNAVNDTSAPWIVSFSSRGPNPETYDILKPDLTAPGVEILAAWSPIATVSSGV 480
           PTATILKSNAVNDTSAPWIVSFSSRGPNPETYDILKPDLTAPGVEILAAWSPIATVSSGV
Sbjct: 421 PTATILKSNAVNDTSAPWIVSFSSRGPNPETYDILKPDLTAPGVEILAAWSPIATVSSGV 480

Query: 481 RDSRTTLYNIISGTSMSCPHATAAAVYVKTFHPTWSPAAIKSALMTTATPLNAKLNTQVE 540
           RDSRTTLYNIISGTSMSCPHATAAAVYVKTFHPTWSPAAIKSALMTTATPLNAKLNTQVE
Sbjct: 481 RDSRTTLYNIISGTSMSCPHATAAAVYVKTFHPTWSPAAIKSALMTTATPLNAKLNTQVE 540

Query: 541 FAYGAGHINPLRAVHPGLLYDAYESDYVRFLCGQGYTTAMVRRLSGDNSVCTRANSGRVW 600
           FAYGAGHINPLRAVHPGLLYDAYESDYVRFLCGQGYTTAMVRRLSGDNSVCTRANSGRVW
Sbjct: 541 FAYGAGHINPLRAVHPGLLYDAYESDYVRFLCGQGYTTAMVRRLSGDNSVCTRANSGRVW 600

Query: 601 DLNYPSFALSSTSSQSFNQFFRRTVTNVGSKVSTYRAKVVGVPRGLSITVNPPVLSFNAI 660
           DLNYPSFALSSTSSQSFNQFFRRTVTNVGSKVSTYRAKVVGVPRGLSITVNPPVLSFNAI
Sbjct: 601 DLNYPSFALSSTSSQSFNQFFRRTVTNVGSKVSTYRAKVVGVPRGLSITVNPPVLSFNAI 660

Query: 661 GQKKSFTLTIRGSISQSIVSASLVWSDGHHNVRSPITVFVVGTA 705
           GQKKSFTLTIRGSISQSIVSASLVWSDGHHNVRSPITVFVVGTA
Sbjct: 661 GQKKSFTLTIRGSISQSIVSASLVWSDGHHNVRSPITVFVVGTA 704

BLAST of Cucsa.164940 vs. TrEMBL
Match: A0A0A0KLY4_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_5G187860 PE=4 SV=1)

HSP 1 Score: 1132.1 bits (2927), Expect = 0.0e+00
Identity = 558/702 (79.49%), Postives = 616/702 (87.75%), Query Frame = 1

Query: 1   MGNKLEDSASTPSHHMRMLEEVVGSSFAPEALLHSYKRSFNGFVVKLTEEEAQKISAKEN 60
           MGNK +D+ASTPSHHMRML EV GS+FAPE+LLHSYKRSFNGFVVKLTEEEA +ISAKE 
Sbjct: 40  MGNKPQDTASTPSHHMRMLREVTGSNFAPESLLHSYKRSFNGFVVKLTEEEAHRISAKEG 99

Query: 61  VVSVFPNEKKHLHTTRSWDFMGFTQKAPRVKQVESNIVVGVLDSGIWPESPSFSDVGYGP 120
           VVSVFP+ KKHLHTTRSWDF+GFT+  PRV QVES+IVVGVLDSGIWPE+PSFSD GYGP
Sbjct: 100 VVSVFPSGKKHLHTTRSWDFIGFTKDVPRVNQVESDIVVGVLDSGIWPENPSFSDAGYGP 159

Query: 121 PPPKWKGACQTSANFHCNRKIIGARAYRSDKFFPPEDIKSPRDSDGHGTHTASTVAGGLV 180
            P KWKG CQ   NF CN+KIIGARAYRSD  FP EDI SPRDS+GHGTHTASTVAGGLV
Sbjct: 160 IPAKWKGICQNPTNFTCNKKIIGARAYRSDNVFPTEDIPSPRDSNGHGTHTASTVAGGLV 219

Query: 181 NQASLYGLALGTARGGVPSARIAVYKICWSDGCYDADILAAFDDAIADGVDIISLSVGGS 240
           +QASLYGLALGTARGGVPSARIAVYKICWSDGC DADILAAFDDAIADGVDIISLSVGGS
Sbjct: 220 SQASLYGLALGTARGGVPSARIAVYKICWSDGCSDADILAAFDDAIADGVDIISLSVGGS 279

Query: 241 KPKYYFNDSIAIGAFHSMKHGILTSNSAGNDGPDYFTIRNFSPWSLSVAASSIDRKLVSR 300
           + +YYFNDSIAIGAFHSMKHGILTSNSAGNDGPDYFTIRNFSPWSLSVAAS+ DRKLVSR
Sbjct: 280 EARYYFNDSIAIGAFHSMKHGILTSNSAGNDGPDYFTIRNFSPWSLSVAASTTDRKLVSR 339

Query: 301 VQLGNKNTFQGYTINTFDLKGKQHPLIYAGSAPNISAGFTGSSSRFCSRNSVDRNLVKGK 360
           V++GN N +QGYTINTFD  GKQ+PLIYAG APN+  GFTGS SRFCS  SVD NLV GK
Sbjct: 340 VEIGNTNVYQGYTINTFDPLGKQYPLIYAGDAPNLIGGFTGSISRFCSEGSVDANLVSGK 399

Query: 361 IVLCDSVLSPATFVSLNGAVGVVMNDLGVKDNARSYPLPSSYLDPVDGDNIKTYMDRTRF 420
           I+LCDS+L+P+ FV  + AVGVVMND GVK  + SYPLPSSYL+ VDGD IKTYM     
Sbjct: 400 ILLCDSILAPSAFVYFSDAVGVVMNDDGVKYPSNSYPLPSSYLETVDGDAIKTYMASNGV 459

Query: 421 PTATILKSNAVNDTSAPWIVSFSSRGPNPETYDILKPDLTAPGVEILAAWSPIATVSSGV 480
           PTATI KS+AVND+SAP+IVSFSSRGPNPET DILKPDLTAPGVEILAAWSPIA VSSGV
Sbjct: 460 PTATIFKSDAVNDSSAPFIVSFSSRGPNPETLDILKPDLTAPGVEILAAWSPIAPVSSGV 519

Query: 481 RDSRTTLYNIISGTSMSCPHATAAAVYVKTFHPTWSPAAIKSALMTTATPLNAKLNTQVE 540
            DSRTTLYNIISGTSMSCPH TAAAVYVKTFHPTWSPAAIKSALMTTATPL  ++N + E
Sbjct: 520 IDSRTTLYNIISGTSMSCPHVTAAAVYVKTFHPTWSPAAIKSALMTTATPLKPEINVEAE 579

Query: 541 FAYGAGHINPLRAVHPGLLYDAYESDYVRFLCGQGYTTAMVRRLSGDNSVCTRANSGRVW 600
           FAYGAG INPL+A+ PGL+YDA E DYV+FLCGQGYT+ MV+ LS DN++C  AN GRVW
Sbjct: 580 FAYGAGQINPLKAISPGLVYDANEFDYVKFLCGQGYTSDMVQSLSNDNTICNSANIGRVW 639

Query: 601 DLNYPSFALSSTSSQSFNQFFRRTVTNVGSKVSTYRAKVVGVPRGLSITVNPPVLSFNAI 660
           DLNYPSFALSST SQS NQFF RT+T+V S  STY + ++G P+GL+ITVNP VLSF+ I
Sbjct: 640 DLNYPSFALSSTPSQSINQFFTRTLTSVDSNASTYTSTILGAPQGLTITVNPKVLSFSGI 699

Query: 661 GQKKSFTLTIRGSIS-QSIVSASLVWSDGHHNVRSPITVFVV 702
           G+KK+FTLTI+G+I   +IVSASLVWSD  H+VRSPIT++VV
Sbjct: 700 GEKKTFTLTIQGTIDPTTIVSASLVWSDSSHDVRSPITIYVV 741

BLAST of Cucsa.164940 vs. TrEMBL
Match: A0A0A0KNZ5_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_5G187870 PE=4 SV=1)

HSP 1 Score: 1086.6 bits (2809), Expect = 0.0e+00
Identity = 530/710 (74.65%), Postives = 609/710 (85.77%), Query Frame = 1

Query: 1   MGNKL-----EDSASTPSHHMRMLEEVVGSSFAPEALLHSYKRSFNGFVVKLTEEEAQKI 60
           MG+KL     +D+ S PSHH R+LE+   S+FAPE LLHSYKRSFNGFV KLTEEEAQKI
Sbjct: 38  MGDKLHDTDSDDTDSAPSHHKRILEKGTSSNFAPEFLLHSYKRSFNGFVAKLTEEEAQKI 97

Query: 61  SAKENVVSVFPNEKKHLHTTRSWDFMGFTQKAPRVKQVESNIVVGVLDSGIWPESPSFSD 120
           SA E VVS+FPNEKKHLHTTRSWDF+G T+ APRVKQVESN+VVGV D+GIWPE+PSFSD
Sbjct: 98  SAMEEVVSLFPNEKKHLHTTRSWDFIGLTKDAPRVKQVESNLVVGVFDTGIWPENPSFSD 157

Query: 121 VGYGPPPPKWKGACQTSANFHCNRKIIGARAYRSDKFFPPEDIKSPRDSDGHGTHTASTV 180
           VGYGP P KWKG CQTSANF CN+KIIGARAYRS+  FPPEDI+SPRDSDGHGTHTASTV
Sbjct: 158 VGYGPIPAKWKGTCQTSANFTCNKKIIGARAYRSNNDFPPEDIRSPRDSDGHGTHTASTV 217

Query: 181 AGGLVNQASLYGLALGTARGGVPSARIAVYKICWSDGCYDADILAAFDDAIADGVDIISL 240
            GGLVN+AS YGLA GTARGG PSA IAVYKICWSDGCY  DILAAFDDAIADGVD+IS+
Sbjct: 218 VGGLVNEASFYGLAGGTARGGTPSACIAVYKICWSDGCYSTDILAAFDDAIADGVDMISI 277

Query: 241 SVGGSKPKYYFNDSIAIGAFHSMKHGILTSNSAGNDGPDYFTIRNFSPWSLSVAASSIDR 300
           S+G  +   YF D  AIGAFH+MK+GILTS SAGN+GP+YF++ N +PW+LSV AS+IDR
Sbjct: 278 SLGSPQSSPYFLDPTAIGAFHAMKNGILTSTSAGNEGPNYFSVSNVAPWALSVGASTIDR 337

Query: 301 KLVSRVQLGNKNTFQGYTINTFDLKGKQHPLIYAGSAPNISAGFTGSSSRFCSRNSVDRN 360
           KL S+V+LGN+N +QG+TINTFDL+GKQ+PLIYA  APNI+ GFTGS SRFCS NSV+ N
Sbjct: 338 KLASKVELGNRNIYQGFTINTFDLEGKQYPLIYARDAPNIAGGFTGSMSRFCSANSVNAN 397

Query: 361 LVKGKIVLCDSVLSPATFVSLNGAVGVVMNDLGVKDNARSYPLPSSYLDPVDGDNIKTYM 420
           LVKGK+++CDSVL P+ FV+ + AVGV+MND   KD++ SYPLPSSYL   DG+N+KTYM
Sbjct: 398 LVKGKVLVCDSVLPPSRFVNFSDAVGVIMNDGRTKDSSGSYPLPSSYLTTADGNNVKTYM 457

Query: 421 DRTRFPTATILKSNAVNDTSAPWIVSFSSRGPNPETYDILKPDLTAPGVEILAAWSPIAT 480
                PTATI KSNA+NDTSAP +VSFSSRGPNP+T+DILKPDLTAPGV+ILAAWSPIA 
Sbjct: 458 SSNGAPTATIYKSNAINDTSAPLVVSFSSRGPNPQTFDILKPDLTAPGVQILAAWSPIAP 517

Query: 481 VSSGVRDSRTTLYNIISGTSMSCPHATAAAVYVKTFHPTWSPAAIKSALMTTATPLNAKL 540
           VSSGV DSRTTLYNIISGTSMSCPH TAAAVYVKTFHPTWSPAAI+SALMTTATPL+A L
Sbjct: 518 VSSGVIDSRTTLYNIISGTSMSCPHVTAAAVYVKTFHPTWSPAAIQSALMTTATPLSAVL 577

Query: 541 NTQVEFAYGAGHINPLRAVHPGLLYDAYESDYVRFLCGQGYTTAMVRRLSGD-NSVCTRA 600
           N Q EFAYGAG I+P++A+ PGL+YDA ESDYV+FLCGQGYTT+MV+R S D N+VC   
Sbjct: 578 NMQAEFAYGAGQIDPVKAIDPGLVYDAGESDYVKFLCGQGYTTSMVQRFSNDKNTVCNST 637

Query: 601 NSGRVWDLNYPSFALSSTSSQSFNQFFRRTVTNVGSKVSTYRAKVVGVPRGLSITVNPPV 660
           N GRVWDLNYPSFALSS+ S+ FNQ+F RT+TNVGSK STY + V G P+GL+ITVNP  
Sbjct: 638 NMGRVWDLNYPSFALSSSPSRPFNQYFTRTLTNVGSKASTYTSTVRGTPQGLTITVNPTS 697

Query: 661 LSFNAIGQKKSFTLTIRGSISQSIVSASLVWSDGHHNVRSPITVFVVGTA 705
           LSFN+ GQK++FTLTIRG++S SI SASL+WSDG HNVRSPITVF V +A
Sbjct: 698 LSFNSTGQKRNFTLTIRGTVSSSIASASLIWSDGSHNVRSPITVFKVASA 747

BLAST of Cucsa.164940 vs. TrEMBL
Match: K7NBW1_SIRGR (Cucumisin OS=Siraitia grosvenorii PE=2 SV=1)

HSP 1 Score: 1013.1 bits (2618), Expect = 1.7e-292
Identity = 502/702 (71.51%), Postives = 584/702 (83.19%), Query Frame = 1

Query: 1   MGNKLEDSASTPSHHMRMLEEVVGSSFAPEALLHSYKRSFNGFVVKLTEEEAQKISAKEN 60
           MG+KLED+AS   +H  MLEEVVGS+FAPE+++++YKRSFNGF VKLTEEEA KI+AKE 
Sbjct: 41  MGSKLEDTASAHLYHRAMLEEVVGSTFAPESVIYTYKRSFNGFAVKLTEEEALKIAAKEG 100

Query: 61  VVSVFPNEKKHLHTTRSWDFMGFTQKAPRVKQVESNIVVGVLDSGIWPESPSFSDVGYGP 120
           VVSVFP+EK HLHTTRSWDF+G +Q  PRVKQVESNIVVGV DSGIWPE+PSF+D G+GP
Sbjct: 101 VVSVFPSEKNHLHTTRSWDFLGISQNVPRVKQVESNIVVGVFDSGIWPENPSFNDDGFGP 160

Query: 121 PPPKWKGACQTSANFHCNRKIIGARAYRSDKFFPPEDIKSPRDSDGHGTHTASTVAGGLV 180
            P  W+G CQ S NF CNRKIIGARAYRS    PP D++SPRD+DGHGTHTASTVAG LV
Sbjct: 161 APANWRGTCQASTNFRCNRKIIGARAYRSSTL-PPGDVRSPRDTDGHGTHTASTVAGVLV 220

Query: 181 NQASLYGLALGTARGGVPSARIAVYKICWSDGCYDADILAAFDDAIADGVDIISLSVGGS 240
           +QASLYGL +GTARGGVP ARIAVYKICWSDGC DADILAAFDDAIADGVDIISLSVGG 
Sbjct: 221 SQASLYGLGVGTARGGVPPARIAVYKICWSDGCSDADILAAFDDAIADGVDIISLSVGGK 280

Query: 241 KPKYYFNDSIAIGAFHSMKHGILTSNSAGNDGPDYFTIRNFSPWSLSVAASSIDRKLVSR 300
            P+ Y  +SIAIG+FH+MK GILTSNSAGN+GP  FT+ + SPW  +VAASS DRK V++
Sbjct: 281 VPQPYLYNSIAIGSFHAMKRGILTSNSAGNNGPKSFTVTSLSPWLPTVAASSSDRKFVTQ 340

Query: 301 VQLGNKNTFQGYTINTFDLKGKQHPLIYAGSAPNISAGFTGSSSRFCSRNSVDRNLVKGK 360
           V LGN NT+QG +INTFD++  Q+PLIYAG+AP+I  GF  S+SR+C  +SVD NLV+GK
Sbjct: 341 VLLGNGNTYQGVSINTFDMR-NQYPLIYAGNAPSI--GFNSSTSRYCYEDSVDPNLVRGK 400

Query: 361 IVLCDSVLSPATFVSLNGAVGVVMNDLGVKDNARSYPLPSSYLDPVDGDNIKTYMDRTRF 420
           I+LCDS   P  F S  GA GV+M     +D+A SYPLP+S LDP  G+NIK YM  TR 
Sbjct: 401 ILLCDSTFGPTVFASFGGAAGVLMQS-NTRDHASSYPLPASVLDPAGGNNIKRYMSSTRA 460

Query: 421 PTATILKSNAVNDTSAPWIVSFSSRGPNPETYDILKPDLTAPGVEILAAWSPIATVSSGV 480
           PTATI KS  V DTSAP +VSFSSRGPN  T+DILKPD TAPGVEILAAW P+A + SGV
Sbjct: 461 PTATIFKSTVVRDTSAPVVVSFSSRGPNYVTHDILKPDSTAPGVEILAAWPPVAPI-SGV 520

Query: 481 RDSRTTLYNIISGTSMSCPHATAAAVYVKTFHPTWSPAAIKSALMTTATPLNAKLNTQVE 540
           RDSR+ LYNIISGTSMSCPH TA AV++KTF+P+WSPAAIKSALMTTA+P+NA+ N+  E
Sbjct: 521 RDSRSALYNIISGTSMSCPHVTAIAVHIKTFYPSWSPAAIKSALMTTASPMNARFNSDAE 580

Query: 541 FAYGAGHINPLRAVHPGLLYDAYESDYVRFLCGQGYTTAMVRRLSGDNSVCTRANSGRVW 600
           FAYG+GH+NPL+AV PGL+YDA ESDYV+FLCG+GYTTAMVR  +GDNS CT  N GRVW
Sbjct: 581 FAYGSGHVNPLKAVDPGLVYDASESDYVKFLCGEGYTTAMVRSTTGDNSACTSGNIGRVW 640

Query: 601 DLNYPSFALSSTSSQSFNQFFRRTVTNVGSKVSTYRAKVVGVPRGLSITVNPPVLSFNAI 660
           DLNYPSFALS + SQ+ NQ FRRT+TNV S  STYRA  +  P+GLSI+VNP VLSFN I
Sbjct: 641 DLNYPSFALSISRSQTANQSFRRTLTNVVSGASTYRAS-ISAPQGLSISVNPSVLSFNGI 700

Query: 661 GQKKSFTLTIRGSISQSIVSASLVWSDGHHNVRSPITVFVVG 703
           G +KSFTLT+RG++SQ+IVSASLVWSDG HNVRSPITV+V+G
Sbjct: 701 GDQKSFTLTVRGTVSQAIVSASLVWSDGSHNVRSPITVYVLG 735

BLAST of Cucsa.164940 vs. TrEMBL
Match: D7TLL6_VITVI (Putative uncharacterized protein OS=Vitis vinifera GN=VIT_13s0019g02480 PE=4 SV=1)

HSP 1 Score: 925.2 bits (2390), Expect = 4.6e-266
Identity = 459/703 (65.29%), Postives = 549/703 (78.09%), Query Frame = 1

Query: 1   MGNKLEDSASTPSHHMRMLEEVVGSSFAPEALLHSYKRSFNGFVVKLTEEEAQKISAKEN 60
           MG+K     S  + H  ML++V GS+ A ++LL+SYKRSFNGFVVKLTEEE +++   + 
Sbjct: 1   MGDKPSGDISAVTAHTNMLQQVFGSNIASDSLLYSYKRSFNGFVVKLTEEEMKELEGMDG 60

Query: 61  VVSVFPNEKKHLHTTRSWDFMGFTQKAPRVKQVESNIVVGVLDSGIWPESPSFSDVGYGP 120
           VVS+FPNEKK LHTTRSWDF+GF Q+  R   VES++++ VLD+GIWPES SF D G+GP
Sbjct: 61  VVSIFPNEKKKLHTTRSWDFIGFPQQVNRTS-VESDVIIAVLDTGIWPESDSFKDKGFGP 120

Query: 121 PPPKWKGACQTSANFHCNRKIIGARAYRSDKFFPPEDIKSPRDSDGHGTHTASTVAGGLV 180
           PP KWKG CQ  +NF CN KIIGAR YRS   F PED+++PRDS+GHGTHTAST AGGLV
Sbjct: 121 PPSKWKGICQGLSNFTCNNKIIGARYYRSYGEFSPEDLQTPRDSEGHGTHTASTAAGGLV 180

Query: 181 NQASLYGLALGTARGGVPSARIAVYKICWSDGCYDADILAAFDDAIADGVDIISLSVGGS 240
           + ASL G  LGTARGGVPSARIAVYKICWSDGC DADILAAFDDAIADGVDIISLSVGGS
Sbjct: 181 SMASLLGFGLGTARGGVPSARIAVYKICWSDGCADADILAAFDDAIADGVDIISLSVGGS 240

Query: 241 KPKYYFNDSIAIGAFHSMKHGILTSNSAGNDGPDYFTIRNFSPWSLSVAASSIDRKLVSR 300
            PK YF DSIAIGAFH+MK+GILTS SAGNDGP++ +I NFSPWSLSVAAS+IDRK  ++
Sbjct: 241 TPKNYFADSIAIGAFHAMKNGILTSTSAGNDGPNFASITNFSPWSLSVAASTIDRKFFTK 300

Query: 301 VQLGNKNTFQGYTINTFDLKGKQHPLIYAGSAPNISAGFTGSSSRFCSRNSVDRNLVKGK 360
           VQLG+   ++G +INTF+  G  +P IY G APNI+ GF+ ++SRFC+RNS+D NLVKGK
Sbjct: 301 VQLGDSKVYEGISINTFEPNG-MYPFIYGGDAPNITGGFSANTSRFCTRNSLDPNLVKGK 360

Query: 361 IVLCDSVLSPATFVSLNGAVGVVMNDLGVKDNARSYPLPSSYLDPVDGDNIKTYMDRTRF 420
           IVLCD + S  T   L GAVG VM D G KD+A  +PLP+SYL   DG +I  Y+  T  
Sbjct: 361 IVLCD-IFSNGTGAFLAGAVGTVMADRGAKDSAWPFPLPASYLGAQDGSSIAYYVTSTSN 420

Query: 421 PTATILKSNAVNDTSAPWIVSFSSRGPNPETYDILKPDLTAPGVEILAAWSPIATVSSGV 480
           PTA+ILKS  VNDT AP+IVSFSSRGPNP T DILKPDL APGV ILAAW PI+ +S   
Sbjct: 421 PTASILKSTEVNDTLAPFIVSFSSRGPNPATLDILKPDLAAPGVHILAAWPPISPISGVQ 480

Query: 481 RDSRTTLYNIISGTSMSCPHATAAAVYVKTFHPTWSPAAIKSALMTTATPLNAKLNTQVE 540
            D+R  LY + SGTSM+CPHAT AA Y+K+FHPTWSPAAIKSALMTTA P++A+ N   E
Sbjct: 481 GDTRAVLYTMQSGTSMACPHATGAAAYIKSFHPTWSPAAIKSALMTTALPMSAEKNPDAE 540

Query: 541 FAYGAGHINPLRAVHPGLLYDAYESDYVRFLCGQGYTTAMVRRLSGDNSVCTRANSGRVW 600
           FAYGAG I+PL++V+PGL+YDA + DYV+FLCGQGYTT  ++ ++GDNSVC+ A +G VW
Sbjct: 541 FAYGAGQIDPLKSVNPGLVYDADKIDYVKFLCGQGYTTQTLQLVTGDNSVCSEATNGTVW 600

Query: 601 DLNYPSFALSSTSSQSFNQFFRRTVTNVGSKVSTYRAKVVGVPRGLSITVNPPVLSFNAI 660
           DLNYPSFALSS++ +S    F RTVTNVGS VSTY+A V G P GL I V P +LSF ++
Sbjct: 601 DLNYPSFALSSSTFESITGVFTRTVTNVGSPVSTYKATVTGAPIGLQIQVVPDILSFTSL 660

Query: 661 GQKKSFTLTIRGSISQSIVSASLVWSDGHHNVRSPITVFVVGT 704
           GQK SF L + G +  +IVSASLVW DG H VRSPI V ++ T
Sbjct: 661 GQKLSFVLKVEGKVGDNIVSASLVWDDGVHQVRSPIVVSILCT 700

BLAST of Cucsa.164940 vs. TAIR10
Match: AT5G59190.1 (AT5G59190.1 subtilase family protein)

HSP 1 Score: 679.9 bits (1753), Expect = 1.7e-195
Identity = 351/705 (49.79%), Postives = 469/705 (66.52%), Query Frame = 1

Query: 1   MGNKLEDSASTPSHHMRMLEEVVGSSFAPEALLHSYKRSFNGFVVKLTEEEAQKISAKEN 60
           MG   E   S PSHH+ +L+++VG+  A   L+ SYKRSFNGF   L++ E+QK+   + 
Sbjct: 1   MGTLPEIKYSPPSHHLSILQKLVGTIAASHLLVRSYKRSFNGFAANLSQAESQKLQNMKE 60

Query: 61  VVSVFPNEKKHLHTTRSWDFMGFTQKAPRVKQVESNIVVGVLDSGIWPESPSFSDVGYGP 120
           VVSVFP++   L TTRSWDF+GF +KA R    ES+++VGV+DSGIWPES SF D G+GP
Sbjct: 61  VVSVFPSKSHELTTTRSWDFVGFGEKARRESVKESDVIVGVIDSGIWPESESFDDEGFGP 120

Query: 121 PPPKWKGACQTSANFHCNRKIIGARAYRSDKFFPPEDIKSPRDSDGHGTHTASTVAGGLV 180
           PP KWKG+C+    F CN K+IGAR Y  +KF       S RD +GHGTHTAST AG  V
Sbjct: 121 PPKKWKGSCKGGLKFACNNKLIGARFY--NKF-----ADSARDEEGHGTHTASTAAGNAV 180

Query: 181 NQASLYGLALGTARGGVPSARIAVYKICWSDGCYDADILAAFDDAIADGVDIISLSVGGS 240
             AS YGLA GTARGGVPSARIA YK+C+ + C D DILAAFDDAIADGVD+IS+S+   
Sbjct: 181 QAASFYGLAQGTARGGVPSARIAAYKVCF-NRCNDVDILAAFDDAIADGVDVISISISAD 240

Query: 241 KPKYYFNDSIAIGAFHSMKHGILTSNSAGNDGPDYFTIRNFSPWSLSVAASSIDRKLVSR 300
                 N S+AIG+FH+M  GI+T+ SAGN+GPD  ++ N SPW ++VAAS  DR+ + R
Sbjct: 241 YVSNLLNASVAIGSFHAMMRGIITAGSAGNNGPDQGSVANVSPWMITVAASGTDRQFIDR 300

Query: 301 VQLGNKNTFQGYTINTFDLKGKQHPLIYAGSAPNISAGFTGSSSRFCSRNSVDRNLVKGK 360
           V LGN     G ++NTF+L G + P++Y     N+S   + + + +CS   VD  LVKGK
Sbjct: 301 VVLGNGKALTGISVNTFNLNGTKFPIVY---GQNVSRNCSQAQAGYCSSGCVDSELVKGK 360

Query: 361 IVLCDSVLSPATFVSLNGAVGVVMNDLGVKDNARSYPLPSSYLDPVDGDNIKTYMDRTRF 420
           IVLCD  L       L GA+GV++ +  + D+A   P P+S L   D  +IK+Y++    
Sbjct: 361 IVLCDDFLGYRE-AYLAGAIGVIVQNTLLPDSAFVVPFPASSLGFEDYKSIKSYIESAEP 420

Query: 421 PTATILKSNAVNDTSAPWIVSFSSRGPNPETYDILKPDLTAPGVEILAAWSPIATVSS-- 480
           P A IL++  + D  AP++ SFSSRGP+    ++LKPD++APG+EILAA+SP+A+ SS  
Sbjct: 421 PQAEILRTEEIVDREAPYVPSFSSRGPSFVIQNLLKPDVSAPGLEILAAFSPVASPSSFL 480

Query: 481 GVRDSRTTLYNIISGTSMSCPHATAAAVYVKTFHPTWSPAAIKSALMTTATPLNAKLNTQ 540
              D R+  Y+++SGTSM+CPH    A YVK+FHP WSP+AIKSA+MTTATP+N K N +
Sbjct: 481 NPEDKRSVRYSVMSGTSMACPHVAGVAAYVKSFHPDWSPSAIKSAIMTTATPMNLKKNPE 540

Query: 541 VEFAYGAGHINPLRAVHPGLLYDAYESDYVRFLCGQGYTTAMVRRLSGDNSVCTRANSGR 600
            EFAYG+G INP +A  PGL+Y+    DY++ LC +G+ +  +   SG N  C+      
Sbjct: 541 QEFAYGSGQINPTKASDPGLVYEVETEDYLKMLCAEGFDSTTLTTTSGQNVTCSERT--E 600

Query: 601 VWDLNYPSFALSSTSSQSFNQFFRRTVTNVGSKVSTYRAKVVGVPRGLSITVNPPVLSFN 660
           V DLNYP+     +S   FN  F+RTVTNVG   STY+A VV +   L I++ P +L F 
Sbjct: 601 VKDLNYPTMTTFVSSLDPFNVTFKRTVTNVGFPNSTYKASVVPLQPELQISIEPEILRFG 660

Query: 661 AIGQKKSFTLTIRGSISQ--SIVSASLVWSDGHHNVRSPITVFVV 702
            + +KKSF +TI G   +  S VS+S+VWSDG H+VRSPI  + +
Sbjct: 661 FLEEKKSFVVTISGKELKDGSFVSSSVVWSDGSHSVRSPIVAYSI 691

BLAST of Cucsa.164940 vs. TAIR10
Match: AT3G46850.1 (AT3G46850.1 Subtilase family protein)

HSP 1 Score: 644.0 bits (1660), Expect = 1.0e-184
Identity = 340/697 (48.78%), Postives = 461/697 (66.14%), Query Frame = 1

Query: 13  SHHMRMLEEVVGSSFAPEALLHSYKRSFNGFVVKLTEEEAQKISAKENVVSVFPNEKKHL 72
           SHH  +L++V G S   + L+ +YKRSFNGF  +LTE E + +++ + VVSVFP++  +L
Sbjct: 51  SHHTSILQDVTGESSIQDRLVRNYKRSFNGFAARLTESEREILASMDEVVSVFPSKNLNL 110

Query: 73  HTTRSWDFMGFTQ--KAPRVKQVESNIVVGVLDSGIWPESPSFSDVGYGPPPPKWKGACQ 132
            TT SW+FMG  +  +  R   +ES+ ++GV+DSGI+PES SFS  G+GPPP KWKG C+
Sbjct: 111 QTTTSWNFMGLKEGKRTKRNPLIESDTIIGVIDSGIYPESDSFSGKGFGPPPKKWKGVCK 170

Query: 133 TSANFHCNRKIIGARAYRSDKFFPPEDIKSPRDSDGHGTHTASTVAGGLVNQASLYGLAL 192
              NF CN K+IGAR Y       PE   S RD+ GHG+HTAS  AG  V   S YGL  
Sbjct: 171 GGTNFTCNNKLIGARYYTPKLEGFPE---SARDNTGHGSHTASIAAGNAVKHVSFYGLGN 230

Query: 193 GTARGGVPSARIAVYKICWSD--GCYDADILAAFDDAIADGVDIISLSVGGSKPKYYFND 252
           GT RGGVP+ARIAVYK+C      C    ILAAFDDAIAD VDII++S+G      +  D
Sbjct: 231 GTVRGGVPAARIAVYKVCDPGVIRCTSDGILAAFDDAIADKVDIITVSLGADAVGTFEED 290

Query: 253 SIAIGAFHSMKHGILTSNSAGNDGPDYFTIRNFSPWSLSVAASSIDRKLVSRVQLGNKNT 312
           ++AIGAFH+M  GILT N AGN+GP+  TI + +PW  +VAAS+++R  +++V LGN  T
Sbjct: 291 TLAIGAFHAMAKGILTVNGAGNNGPERRTIVSMAPWLFTVAASNMNRAFITKVVLGNGKT 350

Query: 313 FQGYTINTFDLKGKQHPLIYAGSAPNISAGFTGSSSRFCSRNSVDRNLVKGKIVLCDSVL 372
             G ++N+FDL GK++PL+Y  SA   S+    SS+ FCS   +D   VKGKIVLCD+  
Sbjct: 351 IVGRSVNSFDLNGKKYPLVYGKSA---SSRCDASSAGFCSPGCLDSKRVKGKIVLCDTQR 410

Query: 373 SPATFVSLNGAVGVVMNDLGVKDNARSYPLPSSYLDPVDGDNIKTYMDRTRFPTATILKS 432
           +P    ++     +V N    +D A  +  P S L   D + + +Y++ T+ P A +LKS
Sbjct: 411 NPGEAQAMGAVASIVRNP--YEDAASVFSFPVSVLSEDDYNIVLSYVNSTKNPKAAVLKS 470

Query: 433 NAVNDTSAPWIVSFSSRGPNPETYDILKPDLTAPGVEILAAWSPIATVSSGVRDSRTTLY 492
             + +  AP + S+SSRGPNP  +DILKPD+TAPG EILAA+SP    S    D+R   Y
Sbjct: 471 ETIFNQKAPVVASYSSRGPNPLIHDILKPDITAPGSEILAAYSPYVPPSES--DTRHVKY 530

Query: 493 NIISGTSMSCPHATAAAVYVKTFHPTWSPAAIKSALMTTATPLNAKL---NTQVEFAYGA 552
            +ISGTSMSCPH    A Y+KTFHP WSP+ I+SA+MTTA P+NA     N   EFAYGA
Sbjct: 531 TVISGTSMSCPHVAGVAAYIKTFHPLWSPSMIQSAIMTTAWPMNASTSPSNELAEFAYGA 590

Query: 553 GHINPLRAVHPGLLYDAYESDYVRFLCGQGYTTAMVRRLSGDNSVCTRANSGRVW-DLNY 612
           GH++P+ A+HPGL+Y+A +SD++ FLCG  YT   +R +SGD+S CT+  +  +  +LNY
Sbjct: 591 GHVDPIAAIHPGLVYEANKSDHITFLCGFNYTGKKLRLISGDSSSCTKEQTKSLTRNLNY 650

Query: 613 PSFALSSTSSQSFNQFFRRTVTNVGSKVSTYRAKVVGVPRGLSITVNPPVLSFNAIGQKK 672
           PS +   + ++ F   FRRTVTNVG   +TY+AKVVG    L + V P VLS  ++ +KK
Sbjct: 651 PSMSAQVSGTKPFKVTFRRTVTNVGRPNATYKAKVVG--SKLKVKVVPAVLSLKSLYEKK 710

Query: 673 SFTLTIRGS--ISQSIVSASLVWSDGHHNVRSPITVF 700
           SFT+T+ G+   ++++VSA L+WSDG H VRSPI V+
Sbjct: 711 SFTVTVSGAGPKAENLVSAQLIWSDGVHFVRSPIVVY 735

BLAST of Cucsa.164940 vs. TAIR10
Match: AT5G59100.1 (AT5G59100.1 Subtilisin-like serine endopeptidase family protein)

HSP 1 Score: 637.1 bits (1642), Expect = 1.3e-182
Identity = 331/697 (47.49%), Postives = 460/697 (66.00%), Query Frame = 1

Query: 13  SHHMRMLEEVVGSSFAPEALLHSYKRSFNGFVVKLTEEEAQKISAKENVVSVFPNEKKHL 72
           S HM +L+E+ G S     L+ SYK+SFNGF  +LTE E ++++  E VVSVFP+ K  L
Sbjct: 52  SDHMSILQEITGESLIENRLVRSYKKSFNGFAARLTESERKRLAGMERVVSVFPSRKLKL 111

Query: 73  HTTRSWDFMGFTQ--KAPRVKQVESNIVVGVLDSGIWPESPSFSDVGYGPPPPKWKGACQ 132
            TT SW+FMG  +  K  R + +ES+ ++GV+DSGI+PES SFSD G+GPPP KWKG C 
Sbjct: 112 QTTSSWNFMGLKEGIKTKRTRSIESDTIIGVIDSGIYPESDSFSDQGFGPPPKKWKGTCA 171

Query: 133 TSANFHCNRKIIGARAYRSDKFFPPEDIKSPRDSDGHGTHTASTVAGGLVNQASLYGLAL 192
              NF CN K+IGAR Y +      +  ++ RD  GHGTHTAS  AG  V  ++ YGL  
Sbjct: 172 GGKNFTCNNKVIGARDYTAKS----KANQTARDYSGHGTHTASIAAGNAVANSNFYGLGN 231

Query: 193 GTARGGVPSARIAVYKICWSDGCYDADILAAFDDAIADGVDIISLSVGGSKPKYYFNDSI 252
           GTARGGVP+ARIAVYK+C ++GC    +++AFDDAIADGVD+IS+S+       +  D I
Sbjct: 232 GTARGGVPAARIAVYKVCDNEGCDGEAMMSAFDDAIADGVDVISISIVLDNIPPFEEDPI 291

Query: 253 AIGAFHSMKHGILTSNSAGNDGPDYFTIRNFSPWSLSVAASSIDRKLVSRVQLGNKNTFQ 312
           AIGAFH+M  G+LT N+AGN+GP   T+ + +PW  SVAAS  +R  +++V LG+     
Sbjct: 292 AIGAFHAMAVGVLTVNAAGNNGPKISTVTSTAPWVFSVAASVTNRAFMAKVVLGDGKILI 351

Query: 313 GYTINTFDLKGKQHPLIYAGSAPNISAGFTGSSSRFCSRNSVDRNLVKGKIVLCDSVLSP 372
           G ++NT+D+ G  +PL+Y  SA   +       +R C    +D  LVKGKIVLCDS    
Sbjct: 352 GRSVNTYDMNGTNYPLVYGKSAALSTCSV--DKARLCEPKCLDGKLVKGKIVLCDSTKGL 411

Query: 373 ATFVSLNGAVGVVMNDLGVKDNARSYPLPSSYLDPVDGDNIKTYMDRTRFPTATILKSNA 432
                L     +V N    +   RS+P+  S+L   D  ++ +YM+ T+ P AT+LKS  
Sbjct: 412 IEAQKLGAVGSIVKNPEPDRAFIRSFPV--SFLSNDDYKSLVSYMNSTKNPKATVLKSEE 471

Query: 433 VNDTSAPWIVSFSSRGPNPETYDILKPDLTAPGVEILAAWSPIATVSSGVRDSRTTLYNI 492
           +++  AP + SFSSRGP+    DILKPD+TAPGVEILAA+SP ++ +    D+R   Y++
Sbjct: 472 ISNQRAPLVASFSSRGPSSIVSDILKPDITAPGVEILAAYSPDSSPTESEFDTRRVKYSV 531

Query: 493 ISGTSMSCPHATAAAVYVKTFHPTWSPAAIKSALMTTATPLNAKLN--TQVEFAYGAGHI 552
           +SGTSM+CPH    A YVKTFHP WSP+ I+SA+MTTA P+NA  +     EFAYG+GH+
Sbjct: 532 LSGTSMACPHVAGVAAYVKTFHPQWSPSMIQSAIMTTAWPMNASGSGFVSTEFAYGSGHV 591

Query: 553 NPLRAVHPGLLYDAYESDYVRFLCGQGYTTAMVRRLSGDNSVCTRANSGRV-WDLNYPSF 612
           +P+ A++PGL+Y+  ++D++ FLCG  YT+  +R +SGDNS CT+  S  +  +LNYP+ 
Sbjct: 592 DPIDAINPGLVYELTKADHINFLCGLNYTSDHLRIISGDNSTCTKEISKTLPRNLNYPTM 651

Query: 613 ALSSTSSQSFNQFFRRTVTNVGSKVSTYRAKVVGVPRG-LSITVNPPVLSFNAIGQKKSF 672
           +   + ++ FN  F+RTVTNVG + STY AKVV  P   LSI V+P VLS  ++ +K+SF
Sbjct: 652 SAKVSGTKPFNITFQRTVTNVGMQKSTYNAKVVKFPGSKLSIKVSPRVLSMKSMNEKQSF 711

Query: 673 TLTIRGSI--SQSIVSASLVWSDGHHNVRSPITVFVV 702
            +T+      ++  VSA+L+WSDG HNVRSPI V+ +
Sbjct: 712 MVTVSSDSIGTKQPVSANLIWSDGTHNVRSPIIVYAM 740

BLAST of Cucsa.164940 vs. TAIR10
Match: AT5G59120.1 (AT5G59120.1 subtilase 4.13)

HSP 1 Score: 636.3 bits (1640), Expect = 2.2e-182
Identity = 342/708 (48.31%), Postives = 458/708 (64.69%), Query Frame = 1

Query: 1   MGNKLEDSASTP-SHHMRMLEEVVGSSFAPEALLHSYKRSFNGFVVKLTEEEAQKISAKE 60
           MG+    +  TP S HM +L+EV G S     L+ SYKRSFNGF  +LTE E ++++   
Sbjct: 36  MGSLSSRADYTPTSDHMNILQEVTGESSIEGRLVRSYKRSFNGFAARLTESERERVAKMV 95

Query: 61  NVVSVFPNEKKHLHTTRSWDFMGFTQ--KAPRVKQVESNIVVGVLDSGIWPESPSFSDVG 120
            VVSVFPN+K  L TT SWDFMG  +  K  R   VES+ ++GV+DSGI PES SFSD G
Sbjct: 96  GVVSVFPNKKLQLQTTTSWDFMGLKEGIKTKRNPTVESDTIIGVIDSGITPESQSFSDKG 155

Query: 121 YGPPPPKWKGACQTSANFHCNRKIIGARAYRSDKFFPPEDIKSPRDSDGHGTHTASTVAG 180
           +GPPP KWKG C    NF CN K+IGAR Y S+           RD DGHGTHTAST AG
Sbjct: 156 FGPPPQKWKGVCSGGKNFTCNNKLIGARDYTSE---------GTRDMDGHGTHTASTAAG 215

Query: 181 GLVNQASLYGLALGTARGGVPSARIAVYKICWSDGCYDADILAAFDDAIADGVDIISLSV 240
             V  AS +G+  GT RGGVP++R+A YK+C   GC    +L+AFDDAIADGVD+I++S+
Sbjct: 216 NAVVDASFFGIGNGTVRGGVPASRVAAYKVCTPTGCSSEALLSAFDDAIADGVDLITISI 275

Query: 241 GGSKPKYYFNDSIAIGAFHSMKHGILTSNSAGNDGPDYFTIRNFSPWSLSVAASSIDRKL 300
           G      + ND IAIGAFH+M  G+LT NSAGN GP   ++   +PW L+VAAS+ +R  
Sbjct: 276 GDKTASMFQNDPIAIGAFHAMAKGVLTVNSAGNSGPKPISVSGVAPWILTVAASTTNRGF 335

Query: 301 VSRVQLGNKNTFQGYTINTFDLKGKQHPLIYAGSAPNISAGFTGSSSRFCSRNSVDRNLV 360
           V++V LGN  T  G ++N +++KGK +PL+Y  SA   S+     S+  C  + VD++ V
Sbjct: 336 VTKVVLGNGKTLVGKSVNAYEMKGKDYPLVYGKSA--ASSACDAESAGLCELSCVDKSRV 395

Query: 361 KGKIVLCDSVLSPATFVSLNGAVGVVMNDLGVKDNARSYPLPSSYLDPVDGDNIKTYMDR 420
           KGKI++C         V   GAVG++       D A  +PLP++ L   D +++ +Y++ 
Sbjct: 396 KGKILVCGGP-GGLKIVESVGAVGLIYR-TPKPDVAFIHPLPAAGLLTEDFESLVSYLES 455

Query: 421 TRFPTATILKSNAVNDTSAPWIVSFSSRGPNPETYDILKPDLTAPGVEILAAWSPIATVS 480
           T  P A +LK+ A+ + ++P I SFSSRGPN    DILKPD+TAPGVEILAA+SP    S
Sbjct: 456 TDSPQAIVLKTEAIFNRTSPVIASFSSRGPNTIAVDILKPDITAPGVEILAAYSPAGEPS 515

Query: 481 SGVRDSRTTLYNIISGTSMSCPHATAAAVYVKTFHPTWSPAAIKSALMTTATPLNAKLN- 540
               D+R   Y+++SGTSMSCPH    A YVKTF+P WSP+ I+SA+MTTA P+NA    
Sbjct: 516 QD--DTRHVKYSVLSGTSMSCPHVAGVAAYVKTFNPKWSPSMIQSAIMTTAWPVNATGTG 575

Query: 541 -TQVEFAYGAGHINPLRAVHPGLLYDAYESDYVRFLCGQGYTTAMVRRLSGDNSVCTRAN 600
               EFAYG+GH++P+ A +PGL+Y+  +SD++ FLCG  YT+ +++ +SG+   C+ A 
Sbjct: 576 IASTEFAYGSGHVDPIAASNPGLVYELDKSDHIAFLCGMNYTSQVLKVISGETVTCSEAK 635

Query: 601 SGRVWDLNYPSF-ALSSTSSQSFNQFFRRTVTNVGSKVSTYRAKVV-GVPRGLSITVNPP 660
                +LNYPS  A  S S  +F   F RT+TNVG+  STY +KVV G    L + + P 
Sbjct: 636 KILPRNLNYPSMSAKLSGSGTTFTVTFNRTLTNVGTPNSTYTSKVVAGHGSKLDVKITPS 695

Query: 661 VLSFNAIGQKKSFTLTIRGSISQSIV--SASLVWSDGHHNVRSPITVF 700
           VLSF  + +K+SFT+T+ GS   S V  SA+L+WSDG HNVRSPI V+
Sbjct: 696 VLSFKTVNEKQSFTVTVTGSNLDSEVPSSANLIWSDGTHNVRSPIVVY 728

BLAST of Cucsa.164940 vs. TAIR10
Match: AT3G46840.1 (AT3G46840.1 Subtilase family protein)

HSP 1 Score: 632.5 bits (1630), Expect = 3.1e-181
Identity = 341/697 (48.92%), Postives = 459/697 (65.85%), Query Frame = 1

Query: 13  SHHMRMLEEVVGSSFAPEALLHSYKRSFNGFVVKLTEEEAQKISAKENVVSVFPNEKKHL 72
           SHH  +L++V G S   + L+ +YKRSFNGF  +LT+ E + +++ + VVSVFPN+K  L
Sbjct: 52  SHHTSILQDVTGESSIEDRLVRNYKRSFNGFAARLTKSEREILASMDEVVSVFPNKKLKL 111

Query: 73  HTTRSWDFMGF--TQKAPRVKQVESNIVVGVLDSGIWPESPSFSDVGYGPPPPKWKGACQ 132
            TT SW+FMG   +++  R   +ES+ ++GV+DSGI+PES SFS  G+GPPP KWKG C+
Sbjct: 112 QTTTSWNFMGLKESKRTKRNTIIESDTIIGVIDSGIYPESDSFSGKGFGPPPKKWKGVCK 171

Query: 133 TSANFHCNRKIIGARAYRSDKFFPPEDIKSPRDSDGHGTHTASTVAGGLVNQASLYGLAL 192
              NF  N K+IGAR Y       PE   S RD  GHG+HTAST AG  V   S YGL  
Sbjct: 172 GGKNFTWNNKLIGARYYTPKLEGFPE---SARDYMGHGSHTASTAAGNAVKHVSFYGLGN 231

Query: 193 GTARGGVPSARIAVYKIC--WSDGCYDADILAAFDDAIADGVDIISLSVGGSKPKYYFND 252
           GTARGGVP+ARIAVYK+C    DGC    ILAAFDDAIAD VDII++S+GG     +  D
Sbjct: 232 GTARGGVPAARIAVYKVCDPGVDGCTTDGILAAFDDAIADKVDIITISIGGDNSSPFEED 291

Query: 253 SIAIGAFHSMKHGILTSNSAGNDGPDYFTIRNFSPWSLSVAASSIDRKLVSRVQLGNKNT 312
            IAIGAFH+M  GIL  NSAGN GP+  T+ + +PW  +VAAS+ +R  V++V LGN  T
Sbjct: 292 PIAIGAFHAMAKGILIVNSAGNSGPEPSTVASIAPWMFTVAASNTNRAFVTKVVLGNGKT 351

Query: 313 FQGYTINTFDLKGKQHPLIYAGSAPNISAGFTGSSSRFCSRNSVDRNLVKGKIVLCDSVL 372
             G ++N+FDL GK++PL+Y  SA   S+    +S+ FCS   +D   VKGKIVLCDS  
Sbjct: 352 V-GRSVNSFDLNGKKYPLVYGKSA---SSSCGAASAGFCSPGCLDSKRVKGKIVLCDSPQ 411

Query: 373 SPATFVSLNGAVGVVMNDLGVKDNARSYPLPSSYLDPVDGDNIKTYMDRTRFPTATILKS 432
           +P    ++     +V +     D A  +  P S L   D + + +YM+ T+ P A +LKS
Sbjct: 412 NPDEAQAMGAIASIVRSHR--TDVASIFSFPVSVLLEDDYNTVLSYMNSTKNPKAAVLKS 471

Query: 433 NAVNDTSAPWIVSFSSRGPNPETYDILKPDLTAPGVEILAAWSPIATVSSGVRDSRTTLY 492
             + +  AP + S+ SRGPN    DILKPD+TAPG EI+AA+SP A  S  + D+R   Y
Sbjct: 472 ETIFNQRAPVVASYFSRGPNTIIPDILKPDITAPGSEIVAAYSPDAPPS--ISDTRRVKY 531

Query: 493 NIISGTSMSCPHATAAAVYVKTFHPTWSPAAIKSALMTTATPLNAK---LNTQVEFAYGA 552
           ++ +GTSMSCPH    A Y+K+FHP WSP+ I+SA+MTTA P+NA     N   EFAYGA
Sbjct: 532 SVDTGTSMSCPHVAGVAAYLKSFHPRWSPSMIQSAIMTTAWPMNASTSPFNELAEFAYGA 591

Query: 553 GHINPLRAVHPGLLYDAYESDYVRFLCGQGYTTAMVRRLSGDNSVCTRANSGRV-WDLNY 612
           GH++P+ A+HPGL+Y+A +SD++ FLCG  YT   +R +SGD+S CT+  +  +  +LNY
Sbjct: 592 GHVDPITAIHPGLVYEANKSDHIAFLCGLNYTAKNLRLISGDSSSCTKEQTKSLPRNLNY 651

Query: 613 PSFALSSTSSQSFNQFFRRTVTNVGSKVSTYRAKVVGVPRGLSITVNPPVLSFNAIGQKK 672
           PS     ++++ F   FRRTVTNVG   +TY+AKVVG    L + V P VLS  ++ +KK
Sbjct: 652 PSMTAQVSAAKPFKVIFRRTVTNVGRPNATYKAKVVG--SKLKVKVVPAVLSLKSLYEKK 711

Query: 673 SFTLTIRGS--ISQSIVSASLVWSDGHHNVRSPITVF 700
           SFT+T  G+   ++++VSA L+WSDG H VRSPI V+
Sbjct: 712 SFTVTASGAGPKAENLVSAQLIWSDGVHFVRSPIVVY 735

BLAST of Cucsa.164940 vs. NCBI nr
Match: gi|700195413|gb|KGN50590.1| (hypothetical protein Csa_5G187880 [Cucumis sativus])

HSP 1 Score: 1419.1 bits (3672), Expect = 0.0e+00
Identity = 704/704 (100.00%), Postives = 704/704 (100.00%), Query Frame = 1

Query: 1   MGNKLEDSASTPSHHMRMLEEVVGSSFAPEALLHSYKRSFNGFVVKLTEEEAQKISAKEN 60
           MGNKLEDSASTPSHHMRMLEEVVGSSFAPEALLHSYKRSFNGFVVKLTEEEAQKISAKEN
Sbjct: 1   MGNKLEDSASTPSHHMRMLEEVVGSSFAPEALLHSYKRSFNGFVVKLTEEEAQKISAKEN 60

Query: 61  VVSVFPNEKKHLHTTRSWDFMGFTQKAPRVKQVESNIVVGVLDSGIWPESPSFSDVGYGP 120
           VVSVFPNEKKHLHTTRSWDFMGFTQKAPRVKQVESNIVVGVLDSGIWPESPSFSDVGYGP
Sbjct: 61  VVSVFPNEKKHLHTTRSWDFMGFTQKAPRVKQVESNIVVGVLDSGIWPESPSFSDVGYGP 120

Query: 121 PPPKWKGACQTSANFHCNRKIIGARAYRSDKFFPPEDIKSPRDSDGHGTHTASTVAGGLV 180
           PPPKWKGACQTSANFHCNRKIIGARAYRSDKFFPPEDIKSPRDSDGHGTHTASTVAGGLV
Sbjct: 121 PPPKWKGACQTSANFHCNRKIIGARAYRSDKFFPPEDIKSPRDSDGHGTHTASTVAGGLV 180

Query: 181 NQASLYGLALGTARGGVPSARIAVYKICWSDGCYDADILAAFDDAIADGVDIISLSVGGS 240
           NQASLYGLALGTARGGVPSARIAVYKICWSDGCYDADILAAFDDAIADGVDIISLSVGGS
Sbjct: 181 NQASLYGLALGTARGGVPSARIAVYKICWSDGCYDADILAAFDDAIADGVDIISLSVGGS 240

Query: 241 KPKYYFNDSIAIGAFHSMKHGILTSNSAGNDGPDYFTIRNFSPWSLSVAASSIDRKLVSR 300
           KPKYYFNDSIAIGAFHSMKHGILTSNSAGNDGPDYFTIRNFSPWSLSVAASSIDRKLVSR
Sbjct: 241 KPKYYFNDSIAIGAFHSMKHGILTSNSAGNDGPDYFTIRNFSPWSLSVAASSIDRKLVSR 300

Query: 301 VQLGNKNTFQGYTINTFDLKGKQHPLIYAGSAPNISAGFTGSSSRFCSRNSVDRNLVKGK 360
           VQLGNKNTFQGYTINTFDLKGKQHPLIYAGSAPNISAGFTGSSSRFCSRNSVDRNLVKGK
Sbjct: 301 VQLGNKNTFQGYTINTFDLKGKQHPLIYAGSAPNISAGFTGSSSRFCSRNSVDRNLVKGK 360

Query: 361 IVLCDSVLSPATFVSLNGAVGVVMNDLGVKDNARSYPLPSSYLDPVDGDNIKTYMDRTRF 420
           IVLCDSVLSPATFVSLNGAVGVVMNDLGVKDNARSYPLPSSYLDPVDGDNIKTYMDRTRF
Sbjct: 361 IVLCDSVLSPATFVSLNGAVGVVMNDLGVKDNARSYPLPSSYLDPVDGDNIKTYMDRTRF 420

Query: 421 PTATILKSNAVNDTSAPWIVSFSSRGPNPETYDILKPDLTAPGVEILAAWSPIATVSSGV 480
           PTATILKSNAVNDTSAPWIVSFSSRGPNPETYDILKPDLTAPGVEILAAWSPIATVSSGV
Sbjct: 421 PTATILKSNAVNDTSAPWIVSFSSRGPNPETYDILKPDLTAPGVEILAAWSPIATVSSGV 480

Query: 481 RDSRTTLYNIISGTSMSCPHATAAAVYVKTFHPTWSPAAIKSALMTTATPLNAKLNTQVE 540
           RDSRTTLYNIISGTSMSCPHATAAAVYVKTFHPTWSPAAIKSALMTTATPLNAKLNTQVE
Sbjct: 481 RDSRTTLYNIISGTSMSCPHATAAAVYVKTFHPTWSPAAIKSALMTTATPLNAKLNTQVE 540

Query: 541 FAYGAGHINPLRAVHPGLLYDAYESDYVRFLCGQGYTTAMVRRLSGDNSVCTRANSGRVW 600
           FAYGAGHINPLRAVHPGLLYDAYESDYVRFLCGQGYTTAMVRRLSGDNSVCTRANSGRVW
Sbjct: 541 FAYGAGHINPLRAVHPGLLYDAYESDYVRFLCGQGYTTAMVRRLSGDNSVCTRANSGRVW 600

Query: 601 DLNYPSFALSSTSSQSFNQFFRRTVTNVGSKVSTYRAKVVGVPRGLSITVNPPVLSFNAI 660
           DLNYPSFALSSTSSQSFNQFFRRTVTNVGSKVSTYRAKVVGVPRGLSITVNPPVLSFNAI
Sbjct: 601 DLNYPSFALSSTSSQSFNQFFRRTVTNVGSKVSTYRAKVVGVPRGLSITVNPPVLSFNAI 660

Query: 661 GQKKSFTLTIRGSISQSIVSASLVWSDGHHNVRSPITVFVVGTA 705
           GQKKSFTLTIRGSISQSIVSASLVWSDGHHNVRSPITVFVVGTA
Sbjct: 661 GQKKSFTLTIRGSISQSIVSASLVWSDGHHNVRSPITVFVVGTA 704

BLAST of Cucsa.164940 vs. NCBI nr
Match: gi|778701133|ref|XP_004149947.2| (PREDICTED: cucumisin-like [Cucumis sativus])

HSP 1 Score: 1419.1 bits (3672), Expect = 0.0e+00
Identity = 704/704 (100.00%), Postives = 704/704 (100.00%), Query Frame = 1

Query: 1   MGNKLEDSASTPSHHMRMLEEVVGSSFAPEALLHSYKRSFNGFVVKLTEEEAQKISAKEN 60
           MGNKLEDSASTPSHHMRMLEEVVGSSFAPEALLHSYKRSFNGFVVKLTEEEAQKISAKEN
Sbjct: 47  MGNKLEDSASTPSHHMRMLEEVVGSSFAPEALLHSYKRSFNGFVVKLTEEEAQKISAKEN 106

Query: 61  VVSVFPNEKKHLHTTRSWDFMGFTQKAPRVKQVESNIVVGVLDSGIWPESPSFSDVGYGP 120
           VVSVFPNEKKHLHTTRSWDFMGFTQKAPRVKQVESNIVVGVLDSGIWPESPSFSDVGYGP
Sbjct: 107 VVSVFPNEKKHLHTTRSWDFMGFTQKAPRVKQVESNIVVGVLDSGIWPESPSFSDVGYGP 166

Query: 121 PPPKWKGACQTSANFHCNRKIIGARAYRSDKFFPPEDIKSPRDSDGHGTHTASTVAGGLV 180
           PPPKWKGACQTSANFHCNRKIIGARAYRSDKFFPPEDIKSPRDSDGHGTHTASTVAGGLV
Sbjct: 167 PPPKWKGACQTSANFHCNRKIIGARAYRSDKFFPPEDIKSPRDSDGHGTHTASTVAGGLV 226

Query: 181 NQASLYGLALGTARGGVPSARIAVYKICWSDGCYDADILAAFDDAIADGVDIISLSVGGS 240
           NQASLYGLALGTARGGVPSARIAVYKICWSDGCYDADILAAFDDAIADGVDIISLSVGGS
Sbjct: 227 NQASLYGLALGTARGGVPSARIAVYKICWSDGCYDADILAAFDDAIADGVDIISLSVGGS 286

Query: 241 KPKYYFNDSIAIGAFHSMKHGILTSNSAGNDGPDYFTIRNFSPWSLSVAASSIDRKLVSR 300
           KPKYYFNDSIAIGAFHSMKHGILTSNSAGNDGPDYFTIRNFSPWSLSVAASSIDRKLVSR
Sbjct: 287 KPKYYFNDSIAIGAFHSMKHGILTSNSAGNDGPDYFTIRNFSPWSLSVAASSIDRKLVSR 346

Query: 301 VQLGNKNTFQGYTINTFDLKGKQHPLIYAGSAPNISAGFTGSSSRFCSRNSVDRNLVKGK 360
           VQLGNKNTFQGYTINTFDLKGKQHPLIYAGSAPNISAGFTGSSSRFCSRNSVDRNLVKGK
Sbjct: 347 VQLGNKNTFQGYTINTFDLKGKQHPLIYAGSAPNISAGFTGSSSRFCSRNSVDRNLVKGK 406

Query: 361 IVLCDSVLSPATFVSLNGAVGVVMNDLGVKDNARSYPLPSSYLDPVDGDNIKTYMDRTRF 420
           IVLCDSVLSPATFVSLNGAVGVVMNDLGVKDNARSYPLPSSYLDPVDGDNIKTYMDRTRF
Sbjct: 407 IVLCDSVLSPATFVSLNGAVGVVMNDLGVKDNARSYPLPSSYLDPVDGDNIKTYMDRTRF 466

Query: 421 PTATILKSNAVNDTSAPWIVSFSSRGPNPETYDILKPDLTAPGVEILAAWSPIATVSSGV 480
           PTATILKSNAVNDTSAPWIVSFSSRGPNPETYDILKPDLTAPGVEILAAWSPIATVSSGV
Sbjct: 467 PTATILKSNAVNDTSAPWIVSFSSRGPNPETYDILKPDLTAPGVEILAAWSPIATVSSGV 526

Query: 481 RDSRTTLYNIISGTSMSCPHATAAAVYVKTFHPTWSPAAIKSALMTTATPLNAKLNTQVE 540
           RDSRTTLYNIISGTSMSCPHATAAAVYVKTFHPTWSPAAIKSALMTTATPLNAKLNTQVE
Sbjct: 527 RDSRTTLYNIISGTSMSCPHATAAAVYVKTFHPTWSPAAIKSALMTTATPLNAKLNTQVE 586

Query: 541 FAYGAGHINPLRAVHPGLLYDAYESDYVRFLCGQGYTTAMVRRLSGDNSVCTRANSGRVW 600
           FAYGAGHINPLRAVHPGLLYDAYESDYVRFLCGQGYTTAMVRRLSGDNSVCTRANSGRVW
Sbjct: 587 FAYGAGHINPLRAVHPGLLYDAYESDYVRFLCGQGYTTAMVRRLSGDNSVCTRANSGRVW 646

Query: 601 DLNYPSFALSSTSSQSFNQFFRRTVTNVGSKVSTYRAKVVGVPRGLSITVNPPVLSFNAI 660
           DLNYPSFALSSTSSQSFNQFFRRTVTNVGSKVSTYRAKVVGVPRGLSITVNPPVLSFNAI
Sbjct: 647 DLNYPSFALSSTSSQSFNQFFRRTVTNVGSKVSTYRAKVVGVPRGLSITVNPPVLSFNAI 706

Query: 661 GQKKSFTLTIRGSISQSIVSASLVWSDGHHNVRSPITVFVVGTA 705
           GQKKSFTLTIRGSISQSIVSASLVWSDGHHNVRSPITVFVVGTA
Sbjct: 707 GQKKSFTLTIRGSISQSIVSASLVWSDGHHNVRSPITVFVVGTA 750

BLAST of Cucsa.164940 vs. NCBI nr
Match: gi|659123579|ref|XP_008461729.1| (PREDICTED: cucumisin-like isoform X1 [Cucumis melo])

HSP 1 Score: 1371.3 bits (3548), Expect = 0.0e+00
Identity = 678/704 (96.31%), Postives = 690/704 (98.01%), Query Frame = 1

Query: 1   MGNKLEDSASTPSHHMRMLEEVVGSSFAPEALLHSYKRSFNGFVVKLTEEEAQKISAKEN 60
           MGNK EDSASTPSHHMRMLEEVVGSSFAPEALLHSYKRSFNGFVVKLTEEEAQKISAKEN
Sbjct: 64  MGNKPEDSASTPSHHMRMLEEVVGSSFAPEALLHSYKRSFNGFVVKLTEEEAQKISAKEN 123

Query: 61  VVSVFPNEKKHLHTTRSWDFMGFTQKAPRVKQVESNIVVGVLDSGIWPESPSFSDVGYGP 120
           VVSVFPNEKKHLHTTRSWDFMGFTQ  PRVKQVESNIVVGVLDSGIWPESPSFSDVGYGP
Sbjct: 124 VVSVFPNEKKHLHTTRSWDFMGFTQNVPRVKQVESNIVVGVLDSGIWPESPSFSDVGYGP 183

Query: 121 PPPKWKGACQTSANFHCNRKIIGARAYRSDKFFPPEDIKSPRDSDGHGTHTASTVAGGLV 180
           PP KWKGACQTSANF CNRKIIGARAYRSDKFFPPEDIKSPRDSDGHGTHTASTVAGGLV
Sbjct: 184 PPAKWKGACQTSANFRCNRKIIGARAYRSDKFFPPEDIKSPRDSDGHGTHTASTVAGGLV 243

Query: 181 NQASLYGLALGTARGGVPSARIAVYKICWSDGCYDADILAAFDDAIADGVDIISLSVGGS 240
           NQASLYGLA GTARGGVPSARIAVYKICWSDGCYDADILAAFDDAIADGVDIISLSVGG+
Sbjct: 244 NQASLYGLASGTARGGVPSARIAVYKICWSDGCYDADILAAFDDAIADGVDIISLSVGGN 303

Query: 241 KPKYYFNDSIAIGAFHSMKHGILTSNSAGNDGPDYFTIRNFSPWSLSVAASSIDRKLVSR 300
           +PKYYFNDSIAIGAFHSMKHGILTSNSAGNDGPDYFTIRNFSPWSLSVAASSIDRKLVSR
Sbjct: 304 RPKYYFNDSIAIGAFHSMKHGILTSNSAGNDGPDYFTIRNFSPWSLSVAASSIDRKLVSR 363

Query: 301 VQLGNKNTFQGYTINTFDLKGKQHPLIYAGSAPNISAGFTGSSSRFCSRNSVDRNLVKGK 360
           VQLGNKN +QGYTINTFDLKGKQ+PLIYAGSAPNIS GFTGSSSRFCSRNSVDRNLVKGK
Sbjct: 364 VQLGNKNIYQGYTINTFDLKGKQYPLIYAGSAPNISGGFTGSSSRFCSRNSVDRNLVKGK 423

Query: 361 IVLCDSVLSPATFVSLNGAVGVVMNDLGVKDNARSYPLPSSYLDPVDGDNIKTYMDRTRF 420
           IVLCDSVLSPATFVSLNGA+GVVMNDLGVKDNARSYPLPSSYL PVDG+NIKTYMDR +F
Sbjct: 424 IVLCDSVLSPATFVSLNGAMGVVMNDLGVKDNARSYPLPSSYLKPVDGNNIKTYMDRNKF 483

Query: 421 PTATILKSNAVNDTSAPWIVSFSSRGPNPETYDILKPDLTAPGVEILAAWSPIATVSSGV 480
           PTATILKSNAVNDTSAPWIVSFSSRGPNPETYDILKPDLTAPGVEILAAWSPIATVSSGV
Sbjct: 484 PTATILKSNAVNDTSAPWIVSFSSRGPNPETYDILKPDLTAPGVEILAAWSPIATVSSGV 543

Query: 481 RDSRTTLYNIISGTSMSCPHATAAAVYVKTFHPTWSPAAIKSALMTTATPLNAKLNTQVE 540
           RDSRTTLYNIISGTSMSCPHATAAAVYVKTFHPTWSPAAIKSALMTTATPLNAKLNTQVE
Sbjct: 544 RDSRTTLYNIISGTSMSCPHATAAAVYVKTFHPTWSPAAIKSALMTTATPLNAKLNTQVE 603

Query: 541 FAYGAGHINPLRAVHPGLLYDAYESDYVRFLCGQGYTTAMVRRLSGDNSVCTRANSGRVW 600
           FAYGAGHINPL+AVHPGLLYDAYE DYVRFLCGQGYTTAMVRRLSGDNS C+RANSGRVW
Sbjct: 604 FAYGAGHINPLKAVHPGLLYDAYERDYVRFLCGQGYTTAMVRRLSGDNSFCSRANSGRVW 663

Query: 601 DLNYPSFALSSTSSQSFNQFFRRTVTNVGSKVSTYRAKVVGVPRGLSITVNPPVLSFNAI 660
           DLNYPSFALSSTS+QSFNQFFRRTVTNVGSKVSTYRAKVVG PRGLSITVNPPVLSFNAI
Sbjct: 664 DLNYPSFALSSTSAQSFNQFFRRTVTNVGSKVSTYRAKVVGAPRGLSITVNPPVLSFNAI 723

Query: 661 GQKKSFTLTIRGSISQSIVSASLVWSDGHHNVRSPITVFVVGTA 705
           GQKKSFTLTIRGSI QSIVSASL+WSDG+HNVRSPITVFVVGTA
Sbjct: 724 GQKKSFTLTIRGSIRQSIVSASLMWSDGYHNVRSPITVFVVGTA 767

BLAST of Cucsa.164940 vs. NCBI nr
Match: gi|659123581|ref|XP_008461730.1| (PREDICTED: cucumisin-like isoform X2 [Cucumis melo])

HSP 1 Score: 1327.4 bits (3434), Expect = 0.0e+00
Identity = 656/683 (96.05%), Postives = 669/683 (97.95%), Query Frame = 1

Query: 22  VVGSSFAPEALLHSYKRSFNGFVVKLTEEEAQKISAKENVVSVFPNEKKHLHTTRSWDFM 81
           +V SSFAPEALLHSYKRSFNGFVVKLTEEEAQKISAKENVVSVFPNEKKHLHTTRSWDFM
Sbjct: 2   MVESSFAPEALLHSYKRSFNGFVVKLTEEEAQKISAKENVVSVFPNEKKHLHTTRSWDFM 61

Query: 82  GFTQKAPRVKQVESNIVVGVLDSGIWPESPSFSDVGYGPPPPKWKGACQTSANFHCNRKI 141
           GFTQ  PRVKQVESNIVVGVLDSGIWPESPSFSDVGYGPPP KWKGACQTSANF CNRKI
Sbjct: 62  GFTQNVPRVKQVESNIVVGVLDSGIWPESPSFSDVGYGPPPAKWKGACQTSANFRCNRKI 121

Query: 142 IGARAYRSDKFFPPEDIKSPRDSDGHGTHTASTVAGGLVNQASLYGLALGTARGGVPSAR 201
           IGARAYRSDKFFPPEDIKSPRDSDGHGTHTASTVAGGLVNQASLYGLA GTARGGVPSAR
Sbjct: 122 IGARAYRSDKFFPPEDIKSPRDSDGHGTHTASTVAGGLVNQASLYGLASGTARGGVPSAR 181

Query: 202 IAVYKICWSDGCYDADILAAFDDAIADGVDIISLSVGGSKPKYYFNDSIAIGAFHSMKHG 261
           IAVYKICWSDGCYDADILAAFDDAIADGVDIISLSVGG++PKYYFNDSIAIGAFHSMKHG
Sbjct: 182 IAVYKICWSDGCYDADILAAFDDAIADGVDIISLSVGGNRPKYYFNDSIAIGAFHSMKHG 241

Query: 262 ILTSNSAGNDGPDYFTIRNFSPWSLSVAASSIDRKLVSRVQLGNKNTFQGYTINTFDLKG 321
           ILTSNSAGNDGPDYFTIRNFSPWSLSVAASSIDRKLVSRVQLGNKN +QGYTINTFDLKG
Sbjct: 242 ILTSNSAGNDGPDYFTIRNFSPWSLSVAASSIDRKLVSRVQLGNKNIYQGYTINTFDLKG 301

Query: 322 KQHPLIYAGSAPNISAGFTGSSSRFCSRNSVDRNLVKGKIVLCDSVLSPATFVSLNGAVG 381
           KQ+PLIYAGSAPNIS GFTGSSSRFCSRNSVDRNLVKGKIVLCDSVLSPATFVSLNGA+G
Sbjct: 302 KQYPLIYAGSAPNISGGFTGSSSRFCSRNSVDRNLVKGKIVLCDSVLSPATFVSLNGAMG 361

Query: 382 VVMNDLGVKDNARSYPLPSSYLDPVDGDNIKTYMDRTRFPTATILKSNAVNDTSAPWIVS 441
           VVMNDLGVKDNARSYPLPSSYL PVDG+NIKTYMDR +FPTATILKSNAVNDTSAPWIVS
Sbjct: 362 VVMNDLGVKDNARSYPLPSSYLKPVDGNNIKTYMDRNKFPTATILKSNAVNDTSAPWIVS 421

Query: 442 FSSRGPNPETYDILKPDLTAPGVEILAAWSPIATVSSGVRDSRTTLYNIISGTSMSCPHA 501
           FSSRGPNPETYDILKPDLTAPGVEILAAWSPIATVSSGVRDSRTTLYNIISGTSMSCPHA
Sbjct: 422 FSSRGPNPETYDILKPDLTAPGVEILAAWSPIATVSSGVRDSRTTLYNIISGTSMSCPHA 481

Query: 502 TAAAVYVKTFHPTWSPAAIKSALMTTATPLNAKLNTQVEFAYGAGHINPLRAVHPGLLYD 561
           TAAAVYVKTFHPTWSPAAIKSALMTTATPLNAKLNTQVEFAYGAGHINPL+AVHPGLLYD
Sbjct: 482 TAAAVYVKTFHPTWSPAAIKSALMTTATPLNAKLNTQVEFAYGAGHINPLKAVHPGLLYD 541

Query: 562 AYESDYVRFLCGQGYTTAMVRRLSGDNSVCTRANSGRVWDLNYPSFALSSTSSQSFNQFF 621
           AYE DYVRFLCGQGYTTAMVRRLSGDNS C+RANSGRVWDLNYPSFALSSTS+QSFNQFF
Sbjct: 542 AYERDYVRFLCGQGYTTAMVRRLSGDNSFCSRANSGRVWDLNYPSFALSSTSAQSFNQFF 601

Query: 622 RRTVTNVGSKVSTYRAKVVGVPRGLSITVNPPVLSFNAIGQKKSFTLTIRGSISQSIVSA 681
           RRTVTNVGSKVSTYRAKVVG PRGLSITVNPPVLSFNAIGQKKSFTLTIRGSI QSIVSA
Sbjct: 602 RRTVTNVGSKVSTYRAKVVGAPRGLSITVNPPVLSFNAIGQKKSFTLTIRGSIRQSIVSA 661

Query: 682 SLVWSDGHHNVRSPITVFVVGTA 705
           SL+WSDG+HNVRSPITVFVVGTA
Sbjct: 662 SLMWSDGYHNVRSPITVFVVGTA 684

BLAST of Cucsa.164940 vs. NCBI nr
Match: gi|700195411|gb|KGN50588.1| (hypothetical protein Csa_5G187860 [Cucumis sativus])

HSP 1 Score: 1132.1 bits (2927), Expect = 0.0e+00
Identity = 558/702 (79.49%), Postives = 616/702 (87.75%), Query Frame = 1

Query: 1   MGNKLEDSASTPSHHMRMLEEVVGSSFAPEALLHSYKRSFNGFVVKLTEEEAQKISAKEN 60
           MGNK +D+ASTPSHHMRML EV GS+FAPE+LLHSYKRSFNGFVVKLTEEEA +ISAKE 
Sbjct: 40  MGNKPQDTASTPSHHMRMLREVTGSNFAPESLLHSYKRSFNGFVVKLTEEEAHRISAKEG 99

Query: 61  VVSVFPNEKKHLHTTRSWDFMGFTQKAPRVKQVESNIVVGVLDSGIWPESPSFSDVGYGP 120
           VVSVFP+ KKHLHTTRSWDF+GFT+  PRV QVES+IVVGVLDSGIWPE+PSFSD GYGP
Sbjct: 100 VVSVFPSGKKHLHTTRSWDFIGFTKDVPRVNQVESDIVVGVLDSGIWPENPSFSDAGYGP 159

Query: 121 PPPKWKGACQTSANFHCNRKIIGARAYRSDKFFPPEDIKSPRDSDGHGTHTASTVAGGLV 180
            P KWKG CQ   NF CN+KIIGARAYRSD  FP EDI SPRDS+GHGTHTASTVAGGLV
Sbjct: 160 IPAKWKGICQNPTNFTCNKKIIGARAYRSDNVFPTEDIPSPRDSNGHGTHTASTVAGGLV 219

Query: 181 NQASLYGLALGTARGGVPSARIAVYKICWSDGCYDADILAAFDDAIADGVDIISLSVGGS 240
           +QASLYGLALGTARGGVPSARIAVYKICWSDGC DADILAAFDDAIADGVDIISLSVGGS
Sbjct: 220 SQASLYGLALGTARGGVPSARIAVYKICWSDGCSDADILAAFDDAIADGVDIISLSVGGS 279

Query: 241 KPKYYFNDSIAIGAFHSMKHGILTSNSAGNDGPDYFTIRNFSPWSLSVAASSIDRKLVSR 300
           + +YYFNDSIAIGAFHSMKHGILTSNSAGNDGPDYFTIRNFSPWSLSVAAS+ DRKLVSR
Sbjct: 280 EARYYFNDSIAIGAFHSMKHGILTSNSAGNDGPDYFTIRNFSPWSLSVAASTTDRKLVSR 339

Query: 301 VQLGNKNTFQGYTINTFDLKGKQHPLIYAGSAPNISAGFTGSSSRFCSRNSVDRNLVKGK 360
           V++GN N +QGYTINTFD  GKQ+PLIYAG APN+  GFTGS SRFCS  SVD NLV GK
Sbjct: 340 VEIGNTNVYQGYTINTFDPLGKQYPLIYAGDAPNLIGGFTGSISRFCSEGSVDANLVSGK 399

Query: 361 IVLCDSVLSPATFVSLNGAVGVVMNDLGVKDNARSYPLPSSYLDPVDGDNIKTYMDRTRF 420
           I+LCDS+L+P+ FV  + AVGVVMND GVK  + SYPLPSSYL+ VDGD IKTYM     
Sbjct: 400 ILLCDSILAPSAFVYFSDAVGVVMNDDGVKYPSNSYPLPSSYLETVDGDAIKTYMASNGV 459

Query: 421 PTATILKSNAVNDTSAPWIVSFSSRGPNPETYDILKPDLTAPGVEILAAWSPIATVSSGV 480
           PTATI KS+AVND+SAP+IVSFSSRGPNPET DILKPDLTAPGVEILAAWSPIA VSSGV
Sbjct: 460 PTATIFKSDAVNDSSAPFIVSFSSRGPNPETLDILKPDLTAPGVEILAAWSPIAPVSSGV 519

Query: 481 RDSRTTLYNIISGTSMSCPHATAAAVYVKTFHPTWSPAAIKSALMTTATPLNAKLNTQVE 540
            DSRTTLYNIISGTSMSCPH TAAAVYVKTFHPTWSPAAIKSALMTTATPL  ++N + E
Sbjct: 520 IDSRTTLYNIISGTSMSCPHVTAAAVYVKTFHPTWSPAAIKSALMTTATPLKPEINVEAE 579

Query: 541 FAYGAGHINPLRAVHPGLLYDAYESDYVRFLCGQGYTTAMVRRLSGDNSVCTRANSGRVW 600
           FAYGAG INPL+A+ PGL+YDA E DYV+FLCGQGYT+ MV+ LS DN++C  AN GRVW
Sbjct: 580 FAYGAGQINPLKAISPGLVYDANEFDYVKFLCGQGYTSDMVQSLSNDNTICNSANIGRVW 639

Query: 601 DLNYPSFALSSTSSQSFNQFFRRTVTNVGSKVSTYRAKVVGVPRGLSITVNPPVLSFNAI 660
           DLNYPSFALSST SQS NQFF RT+T+V S  STY + ++G P+GL+ITVNP VLSF+ I
Sbjct: 640 DLNYPSFALSSTPSQSINQFFTRTLTSVDSNASTYTSTILGAPQGLTITVNPKVLSFSGI 699

Query: 661 GQKKSFTLTIRGSIS-QSIVSASLVWSDGHHNVRSPITVFVV 702
           G+KK+FTLTI+G+I   +IVSASLVWSD  H+VRSPIT++VV
Sbjct: 700 GEKKTFTLTIQGTIDPTTIVSASLVWSDSSHDVRSPITIYVV 741