Cucsa.163790 (gene) Cucumber (Gy14) v1

BLAST of Cucsa.163790 vs. Swiss-Prot
Match: DNJ49_ARATH (Chaperone protein dnaJ 49 OS=Arabidopsis thaliana GN=ATJ49 PE=2 SV=2)

HSP 1 Score: 72.0 bits (175), Expect = 3.5e-11
Identity = 50/161 (31.06%), Postives = 77/161 (47.83%), Query Frame = 1

Query: 1   MECNKEEAIKAMKIAETKLEISDFIGARKMAQTAQRLFPTLDNITQLLTVCEIHCSAQNR 60
           M+ NK++A + ++IAE  +   D   A K    A+RL P+L ++ +L+  C+   S    
Sbjct: 1   MDGNKDDASRCLRIAEDAIVSGDKERALKFINMAKRLNPSL-SVDELVAACDNLDSVSRN 60

Query: 61  MYGAE---------------------------------NDWYGILQIEQSADEAIIKKQY 120
              +E                                 ND+Y IL +E++     I+K Y
Sbjct: 61  SSVSEKLKTMDGDDDKLETGKMKYTEENVDLVRNIIRNNDYYAILGLEKNCSVDEIRKAY 120

Query: 121 RKLALLLHPDKNKFAGAEAAFKLVGEANRLLSDQSKRKLYD 129
           RKL+L +HPDKNK  G+E AFK V +A   LSD + R+ +D
Sbjct: 121 RKLSLKVHPDKNKAPGSEEAFKKVSKAFTCLSDGNSRRQFD 160

BLAST of Cucsa.163790 vs. Swiss-Prot
Match: DJB12_MOUSE (DnaJ homolog subfamily B member 12 OS=Mus musculus GN=Dnajb12 PE=1 SV=2)

HSP 1 Score: 71.6 bits (174), Expect = 4.6e-11
Identity = 61/198 (30.81%), Postives = 87/198 (43.94%), Query Frame = 1

Query: 1   MECNKEEAIKAMKIAETKLEISDFIGARKMAQTAQRLFPT------LDNITQLLTVCEIH 60
           ME NK+EA + + IA   ++ +    A +  + AQRL+PT      ++++ Q       H
Sbjct: 1   MESNKDEAERCISIALKAIQSNQPERALRFLEKAQRLYPTPRVSALIESLNQKPQSTGDH 60

Query: 61  C-------------------SAQNRMYGAEN-------------------DWYGILQIEQ 120
                               SA     G E+                   D+Y IL + +
Sbjct: 61  PQPTDTTHTTTKKAGGTETPSANGEAGGGESAKGYTSEQVAAVKRVKQCKDYYEILGVSR 120

Query: 121 SADEAIIKKQYRKLALLLHPDKNKFAGAEAAFKLVGEANRLLSDQSKRKLYDLKYGAARR 154
           SA +  +KK YRKLAL  HPDKN   GA  AFK +G A  +LS+  KRK YD ++G  + 
Sbjct: 121 SASDEDLKKAYRKLALKFHPDKNHAPGATEAFKAIGTAYAVLSNPEKRKQYD-QFGDDKS 180

BLAST of Cucsa.163790 vs. Swiss-Prot
Match: DNAJ_MYCGA (Chaperone protein DnaJ OS=Mycoplasma gallisepticum (strain R(low / passage 15 / clone 2)) GN=dnaJ PE=3 SV=2)

HSP 1 Score: 71.2 bits (173), Expect = 6.0e-11
Identity = 34/65 (52.31%), Postives = 47/65 (72.31%), Query Frame = 1

Query: 64  AENDWYGILQIEQSADEAIIKKQYRKLALLLHPDKNKFAGAEAAFKLVGEANRLLSDQSK 123
           ++ D+Y IL++ +SA +  IKK +RKLA+  HPD+NK + AE  FK V EA  +LSD+ K
Sbjct: 3   SKRDYYEILEVSRSATQQDIKKAFRKLAMKYHPDRNKDSDAEEKFKEVNEAYEVLSDEEK 62

Query: 124 RKLYD 129
           RKLYD
Sbjct: 63  RKLYD 67

BLAST of Cucsa.163790 vs. Swiss-Prot
Match: DJB14_XENLA (DnaJ homolog subfamily B member 14 OS=Xenopus laevis GN=dnajb14 PE=2 SV=1)

HSP 1 Score: 70.5 bits (171), Expect = 1.0e-10
Identity = 53/164 (32.32%), Postives = 73/164 (44.51%), Query Frame = 1

Query: 1   MECNKEEAIKAMKIAETKLEISDFIGARKMAQTAQRLFPT------LDNITQLLTVCE-- 60
           ME N++EA + ++I +  +E  D   AR+    A+RL+P+      LD + +  T     
Sbjct: 1   MESNRDEAERCVRIGKAAIEAGDKEKARRFFSKAERLYPSSEARVLLDALEKNDTAGNGP 60

Query: 61  --IHCSAQNRMYGAEND-------------------------WYGILQIEQSADEAIIKK 120
                S       AE D                         +Y +L +   A E  +KK
Sbjct: 61  QSEKMSKSTEQPKAEKDSSGDTGKGHTQDQVDGVQRIKKCKTYYEVLGVSPDAGEEDLKK 120

Query: 121 QYRKLALLLHPDKNKFAGAEAAFKLVGEANRLLSDQSKRKLYDL 130
            YRKLAL  HPDKN   GA  AFK +G A  +LS+  KRK YDL
Sbjct: 121 AYRKLALKFHPDKNHAPGATEAFKKIGNAYAVLSNPEKRKQYDL 164

BLAST of Cucsa.163790 vs. Swiss-Prot
Match: DNJA6_ARATH (Chaperone protein dnaJ A6, chloroplastic OS=Arabidopsis thaliana GN=DJA6 PE=2 SV=2)

HSP 1 Score: 68.9 bits (167), Expect = 3.0e-10
Identity = 42/113 (37.17%), Postives = 56/113 (49.56%), Query Frame = 1

Query: 64  AENDWYGILQIEQSADEAIIKKQYRKLALLLHPDKNKFAGAEAAFKLVGEANRLLSDQSK 123
           A+ D+Y +L + ++A +A IK  YRKLA   HPD NK  GAE  FK +  A  +LSD  K
Sbjct: 83  ADADYYSVLGVSKNATKAEIKSAYRKLARNYHPDVNKDPGAEEKFKEISNAYEVLSDDEK 142

Query: 124 RKLYDLKYGAARRNIAPAKSSHDQQNGYTAVNKQERGTANGYSSGPFSHYPGG 177
           + LYD +YG A    A    + D  N +   +    G   G   G  S    G
Sbjct: 143 KSLYD-RYGEAGLKGAAGFGNGDFSNPFDLFDSLFEGFGGGMGRGSRSRAVDG 194

BLAST of Cucsa.163790 vs. TrEMBL
Match: A0A0A0LYL2_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_1G572990 PE=4 SV=1)

HSP 1 Score: 1734.5 bits (4491), Expect = 0.0e+00
Identity = 846/847 (99.88%), Postives = 847/847 (100.00%), Query Frame = 1

Query: 1   MECNKEEAIKAMKIAETKLEISDFIGARKMAQTAQRLFPTLDNITQLLTVCEIHCSAQNR 60
           MECNKEEAIKAMKIAETKLEISDFIGARKMAQTAQRLFPTLDNITQLLTVCEIHCSAQNR
Sbjct: 1   MECNKEEAIKAMKIAETKLEISDFIGARKMAQTAQRLFPTLDNITQLLTVCEIHCSAQNR 60

Query: 61  MYGAENDWYGILQIEQSADEAIIKKQYRKLALLLHPDKNKFAGAEAAFKLVGEANRLLSD 120
           MYGAENDWYGILQIEQSADEAIIKKQYRKLALLLHPDKNKFAGAEAAFKLVGEANRLLSD
Sbjct: 61  MYGAENDWYGILQIEQSADEAIIKKQYRKLALLLHPDKNKFAGAEAAFKLVGEANRLLSD 120

Query: 121 QSKRKLYDLKYGAARRNIAPAKSSHDQQNGYTAVNKQERGTANGYSSGPFSHYPGGNSFK 180
           QSKRKLYDLKYGAARRNIAPAKSSHDQQNGYTAVNKQERGTANGYSSGPFSHYPGGNSFK
Sbjct: 121 QSKRKLYDLKYGAARRNIAPAKSSHDQQNGYTAVNKQERGTANGYSSGPFSHYPGGNSFK 180

Query: 181 PPQPPAQQAFWTCCPFCNVRYQYLKCYLSKMLRCQNCGRGFISHDLNNQTIPPTFHQMHV 240
           PPQPPAQQAFWTCCPFCNVRYQYLKCYLSKMLRCQNCGRGFISHDLNNQTIPPTFHQM+V
Sbjct: 181 PPQPPAQQAFWTCCPFCNVRYQYLKCYLSKMLRCQNCGRGFISHDLNNQTIPPTFHQMNV 240

Query: 241 PQKKVAPESGPSKPAAENKQGSVKKSQDRSGGVDLNAKAGKKQKGQGSNAKPKADAEKTG 300
           PQKKVAPESGPSKPAAENKQGSVKKSQDRSGGVDLNAKAGKKQKGQGSNAKPKADAEKTG
Sbjct: 241 PQKKVAPESGPSKPAAENKQGSVKKSQDRSGGVDLNAKAGKKQKGQGSNAKPKADAEKTG 300

Query: 301 KEKAKSDATSTEKVATKSQNRKRQRKSATAHGNNSEHGDDEVEVDNVSEKDPGLSRDNCQ 360
           KEKAKSDATSTEKVATKSQNRKRQRKSATAHGNNSEHGDDEVEVDNVSEKDPGLSRDNCQ
Sbjct: 301 KEKAKSDATSTEKVATKSQNRKRQRKSATAHGNNSEHGDDEVEVDNVSEKDPGLSRDNCQ 360

Query: 361 RRSTRNKRQVSYRKYLNEDDDSLQSPNKSSGTASTDLKEEMKDATSNVEASAKGMKQEVL 420
           RRSTRNKRQVSYRKYLNEDDDSLQSPNKSSGTASTDLKEEMKDATSNVEASAKGMKQEVL
Sbjct: 361 RRSTRNKRQVSYRKYLNEDDDSLQSPNKSSGTASTDLKEEMKDATSNVEASAKGMKQEVL 420

Query: 421 PPHPEDSPNRKPKCEEVLREGKNGSDKNDNKSKTEIVDTEENGLQGGVHVLVCADPEFSD 480
           PPHPEDSPNRKPKCEEVLREGKNGSDKNDNKSKTEIVDTEENGLQGGVHVLVCADPEFSD
Sbjct: 421 PPHPEDSPNRKPKCEEVLREGKNGSDKNDNKSKTEIVDTEENGLQGGVHVLVCADPEFSD 480

Query: 481 FDTDKGKDCFAVNQVWAIYDTVDGMPRFYARIRKVFSPEFKLQISWFEPHPDDKGEIEWC 540
           FDTDKGKDCFAVNQVWAIYDTVDGMPRFYARIRKVFSPEFKLQISWFEPHPDDKGEIEWC
Sbjct: 481 FDTDKGKDCFAVNQVWAIYDTVDGMPRFYARIRKVFSPEFKLQISWFEPHPDDKGEIEWC 540

Query: 541 DAELPIACGKYTLGGSELTAELPMFSHMVHCPKQGASKSSYFMYPRKGETWALFKDWDIR 600
           DAELPIACGKYTLGGSELTAELPMFSHMVHCPKQGASKSSYFMYPRKGETWALFKDWDIR
Sbjct: 541 DAELPIACGKYTLGGSELTAELPMFSHMVHCPKQGASKSSYFMYPRKGETWALFKDWDIR 600

Query: 601 WSSEPEKHVAFEFEFVEILSDYVEGVGISVAFMDKVKDFVCLFHTTEKHRQNSFKIPPNE 660
           WSSEPEKHVAFEFEFVEILSDYVEGVGISVAFMDKVKDFVCLFHTTEKHRQNSFKIPPNE
Sbjct: 601 WSSEPEKHVAFEFEFVEILSDYVEGVGISVAFMDKVKDFVCLFHTTEKHRQNSFKIPPNE 660

Query: 661 LYRFSHQIPSVRMTGKERKGVPKGSFELDPAALPPNINDEHVDLNNVKEETNDAPASSGK 720
           LYRFSHQIPSVRMTGKERKGVPKGSFELDPAALPPNINDEHVDLNNVKEETNDAPASSGK
Sbjct: 661 LYRFSHQIPSVRMTGKERKGVPKGSFELDPAALPPNINDEHVDLNNVKEETNDAPASSGK 720

Query: 721 TDSSHGFKSPKEKVEVIVLDNNEAAKIQKSNLKKSHPNSEVPTTVRKSPRKLNLTESDAQ 780
           TDSSHGFKSPKEKVEVIVLDNNEAAKIQKSNLKKSHPNSEVPTTVRKSPRKLNLTESDAQ
Sbjct: 721 TDSSHGFKSPKEKVEVIVLDNNEAAKIQKSNLKKSHPNSEVPTTVRKSPRKLNLTESDAQ 780

Query: 781 VDKFVPEDNRSRDGSRNGLSTHKESSAIHQNGGTSTPKKHGESSGLRGTTCLRIRKSPRD 840
           VDKFVPEDNRSRDGSRNGLSTHKESSAIHQNGGTSTPKKHGESSGLRGTTCLRIRKSPRD
Sbjct: 781 VDKFVPEDNRSRDGSRNGLSTHKESSAIHQNGGTSTPKKHGESSGLRGTTCLRIRKSPRD 840

Query: 841 LSKKNAG 848
           LSKKNAG
Sbjct: 841 LSKKNAG 847

BLAST of Cucsa.163790 vs. TrEMBL
Match: F6HTY9_VITVI (Putative uncharacterized protein OS=Vitis vinifera GN=VIT_14s0030g00640 PE=4 SV=1)

HSP 1 Score: 674.1 bits (1738), Expect = 2.2e-190
Identity = 401/901 (44.51%), Postives = 544/901 (60.38%), Query Frame = 1

Query: 1   MECNKEEAIKAMKIAETKLEISDFIGARKMAQTAQRLFPTLDNITQLLTVCEIHCSAQNR 60
           M+CNKEEA++A  I+E K++  DFIGAR++AQ AQ+LFP L+NI+QLLTVC++HCSAQN+
Sbjct: 1   MDCNKEEALRAKVISEKKMQSGDFIGARRIAQRAQQLFPDLENISQLLTVCDVHCSAQNK 60

Query: 61  MYGAENDWYGILQIEQSADEAIIKKQYRKLALLLHPDKNKFAGAEAAFKLVGEANRLLSD 120
           +YG E DWYGIL++EQ+AD+AIIKKQYRKLALLLHPDKNKFAGAEAAFKL+GEANR+LSD
Sbjct: 61  IYGTEMDWYGILKVEQAADDAIIKKQYRKLALLLHPDKNKFAGAEAAFKLIGEANRILSD 120

Query: 121 QSKRKLYDLKYGAARRNIAPAKSSHDQQNGYTAVNKQERGTANGYSSGPFSHYPGGNSFK 180
           Q KR  YD+KY  + ++ AP    H Q N  + V KQ  G  N + +    H  G N  +
Sbjct: 121 QGKRSAYDMKYRVSLKHTAPKPPPH-QLNRNSFVRKQ-YGVQNNFPNVANPHGVGLNPHQ 180

Query: 181 PPQP---PAQQAFWTCCPFCNVRYQYLKCYLSKMLRCQNCGRGFISHDLNNQTIPP--TF 240
             QP     QQ FWTCCPFC++RYQY +  ++++LRCQ C + FI++DL  Q++PP  T+
Sbjct: 181 QTQPGLSDGQQTFWTCCPFCSIRYQYYRDIMNRVLRCQTCQKSFIAYDLGAQSVPPGATW 240

Query: 241 HQ----MH--VPQK---KVAPESGPSKPAAENKQGSVKKSQDRSGGVDLNAK---AGKKQ 300
            Q    +H  VP +   KV  +S    P +   QGS      ++ G DL  K   A +  
Sbjct: 241 SQPAFSLHNEVPNQCPIKVKTQSPAMNPGSMGSQGSF---NSKTAGPDLVKKKRCADEAI 300

Query: 301 KGQGSNAKPKADAE---KTGKEKAKSDATSTEKVATKSQNRKRQRKSATAHGNNS---EH 360
            G  +N K   + +   K G    KSDA    K  +  +N  R+RK+     + S     
Sbjct: 301 GGSKTNGKEDGNVDVGSKKGVRMPKSDADKPRKSGSSRRNTSRKRKNLPVESSESCQTSS 360

Query: 361 GDDEVEVDNVSEKDPGLSRDNCQ-------RRSTRNKRQVSYRKYLNEDDDSLQSPNKSS 420
            +D  E     EK    S +N +       RRS+R K+ V Y + +++DDD +  P K+ 
Sbjct: 361 SEDAKEAAIAQEKGVVPSGENSEFNIGHQPRRSSRKKQHVYYNESVSDDDDFVSPPKKAR 420

Query: 421 GTASTDLKEEMKDA---------------TSNVEASAKGMKQEVLPPHPEDSPNRKPKCE 480
              S    EE KD                TS V+   + +KQ+   P  E    RK +  
Sbjct: 421 MDGSLGTGEERKDKPLDDGVPKTCNTAGFTSVVDVGKENIKQKENVPLEETVVKRKSEAG 480

Query: 481 EVLREGKNGSDKNDNKSKTE-IVDTEENGLQGGVH---VLVCADPEFSDFDTDKGKDCFA 540
             +  GK  +  +DN  + +  V++E N      H    L C D +FSDFD DK +DCF+
Sbjct: 481 GCMINGKAAATADDNDERCKGSVNSEPNSCPDVTHEPVSLECLDCDFSDFDKDKREDCFS 540

Query: 541 VNQVWAIYDTVDGMPRFYARIRKVFSPEFKLQISWFEPHPDDKGEIEWCDAELPIACGKY 600
           V+Q+WAIYD +DGMPRFYARIRKVF+PEFKL+ +W EP PDD  EI W   ELP ACGK+
Sbjct: 541 VDQIWAIYDPIDGMPRFYARIRKVFAPEFKLRFTWLEPSPDDASEIAWVKNELPYACGKF 600

Query: 601 TLGGSELTAELPMFSHMVHCPKQGASKSSYFMYPRKGETWALFKDWDIRWSSEPEKHVAF 660
           T G +E TA+LPMFSH VH  K G  ++SYF+YPRKGETWA++K+W+  WSS PE H  +
Sbjct: 601 TYGQTEETADLPMFSHQVHGEK-GGIRNSYFVYPRKGETWAIYKNWNTDWSSNPEIHRKY 660

Query: 661 EFEFVEILSDYVEGVGISVAFMDKVKDFVCLFHTTEKHRQNSFKIPPNELYRFSHQIPSV 720
           EFE+VEILSD+V   GI VA++ KVK FV LF  + +H    F+IPP+EL RFSH+IPS 
Sbjct: 661 EFEYVEILSDFVPDAGIGVAYLGKVKGFVSLFRQSVQHGIVLFQIPPSELLRFSHRIPSF 720

Query: 721 RMTGKERKGVPKGSFELDPAALPPNIND--EHVDLNNVKEETNDAPASSGKTDSSHGFKS 780
           RMTG E +GVPKGSFELDPAALP N+ND   + DL   KE  N A  +  +T      KS
Sbjct: 721 RMTGSEGEGVPKGSFELDPAALPNNLNDFSGNDDLKTEKESVN-AGVNGSRT------KS 780

Query: 781 PKEKVEVIVLDNNEAAKIQKSNLKKSHPNSEVPTTVRKSPRKLN-LTESDAQVDKFVPED 840
           P+ +++ +    N    I+    +++    E  + +R+SPR+LN L + D QV++     
Sbjct: 781 PENEMKSM----NNPTMIKPMKHEENDTERET-SELRRSPRELNGLYKKDGQVNQSECA- 840

Query: 841 NRSRDGSRNGLSTHKESSAIHQN---GGTSTPKKHGESSGLRGTTCLRIRKSPRDLSKKN 847
           N++  G +N     +   +I+ N      +TPKKH E   L  T   ++R+SPR L+KK+
Sbjct: 841 NQAEIGDKNHGDLTQSKGSIYVNLADERINTPKKH-EKDDLE-TGNFKLRRSPRALNKKH 880

BLAST of Cucsa.163790 vs. TrEMBL
Match: F6HTY9_VITVI (Putative uncharacterized protein OS=Vitis vinifera GN=VIT_14s0030g00640 PE=4 SV=1)

HSP 1 Score: 108.6 bits (270), Expect = 3.8e-20
Identity = 70/224 (31.25%), Postives = 114/224 (50.89%), Query Frame = 1

Query: 475  DPEFSDFDTDKGKDCFAVNQVWAIYDTVDGMPRFYARIRKVF-SPEFKLQISWFEPHPDD 534
            + +F DF  +K ++ F   Q+WA+Y  VD MP+ YA+++K+  +P F+L + + E     
Sbjct: 952  EAQFYDFSGEKSEEKFQTGQLWALYSEVDRMPKNYAQVKKIEPTPSFRLHVVFLEACSPP 1011

Query: 535  KGEIEWCDAELPIACGKYTL-GGSELTAELPMFSHMVHCPKQGASKSSYFMYPRKGETWA 594
            K  ++      P+ CG + L  G         FSH +     G  K+ + + P KG+ WA
Sbjct: 1012 KDMVQ------PVCCGTFKLKNGKTKVFPRADFSHQIRAESIG--KNKFAILPIKGQVWA 1071

Query: 595  LFKDWDIRWSSEPEKHVAFEFEFVEILSDYVEGVGISVAFMDKVKDFVCLFHTTEKHRQN 654
            L+K+W+          V  +++ VE+L D      +SV     +  F  ++    + R +
Sbjct: 1072 LYKNWENNLMC--SDIVNCKYDIVEVLEDNDHSTKVSVLL--PLNGFKSVYKAPRRQRSS 1131

Query: 655  S--FKIPPNELYRFSHQIPSVRMTGKERKGVPKGSFELDPAALP 695
            +    IP +EL RFSHQIP+VR TG E        +ELDPA++P
Sbjct: 1132 TGILDIPRDELPRFSHQIPAVRHTG-ENDARLADCWELDPASVP 1162

HSP 2 Score: 603.6 bits (1555), Expect = 3.7e-169
Identity = 353/858 (41.14%), Postives = 488/858 (56.88%), Query Frame = 1

Query: 1   MECNKEEAIKAMKIAETKLEISDFIGARKMAQTAQRLFPTLDNITQLLTVCEIHCSAQNR 60
           MECNK+EAI+A +IAE K++  DF G  K A  AQRLFP + NI Q+L VCE+HC+AQ +
Sbjct: 1   MECNKDEAIRARQIAENKMQAGDFEGGLKFATKAQRLFPEIQNILQILAVCEVHCAAQKK 60

Query: 61  MYGAENDWYGILQIEQSADEAIIKKQYRKLALLLHPDKNKFAGAEAAFKLVGEANRLLSD 120
             G++ DWYGIL+IE+SADEA IKKQYRKLALLLHPDKNK  GAEAAFKL+GEANR+LSD
Sbjct: 61  HSGSDMDWYGILKIEKSADEATIKKQYRKLALLLHPDKNKSDGAEAAFKLIGEANRVLSD 120

Query: 121 QSKRKLYDLKYGAARRNIAPAKSSHDQQNGYTAVNKQERGTANGYSSGPFSHYPGGNSFK 180
           Q+KR LYDLK+G    N A AK      NG  A      GTA  Y +   S Y   NS+ 
Sbjct: 121 QTKRALYDLKFGVPVGNTA-AKVPPRHPNG-NASGMGCDGTARNYQNSFSSQYQAWNSYH 180

Query: 181 PPQPPAQQAFWTCCPFCNVRYQYLKCYLSKMLRCQNCGRGFISHDLNNQTIPPTFHQMHV 240
                  Q FWTCCP CN RYQY+   L+  +RCQ+C + F +HD+ N  + P +     
Sbjct: 181 RTD---NQTFWTCCPHCNTRYQYVITILNHTIRCQHCSKSFTAHDMGNHNVSPGYWSPFN 240

Query: 241 PQKKVAPESGPSKPAAENKQGSVKKSQDR-------SGGVDLNAKAGKKQKGQGSNAKPK 300
            QK+  P+   SK A+++  G     +         S G+  ++K  K++    +    K
Sbjct: 241 NQKE-PPKHASSKEASKSNGGKSSGREQEGVSMSKCSAGIGTHSKVAKRRDSHAAAGVTK 300

Query: 301 ADAEKTGKEKAKSDATSTEKVATKSQNRKRQRKSATAHGNNSEHGDDEVEVDNVSEKDPG 360
           A    +     K    + E  A+     KR R+S +   N + +G        V +    
Sbjct: 301 AGVGMSNPTNTK----AKESQASTKVGHKRARQSTSDDDNKAANG------KGVKDAKVQ 360

Query: 361 LSRDNCQRRSTRNKRQVSYRKYLNEDDDSLQSPNKSSGTASTDLKEEMKDATSNVEASAK 420
            +R +  RRS+R K+ VSY +   + D    S       +S +      D          
Sbjct: 361 KNRVDPNRRSSRKKQHVSYTENDKDGDFGNSSKRPRHHESSNNNPASFTDGVGGQNGK-- 420

Query: 421 GMKQEVLPPHPEDSPNRKPKCEE--VLREGKNGSDKNDNKSKTEIVDTEENGLQGGVHVL 480
            ++ +   P  E     K K E+  VLR+  + SD ND KSK +     ++ L     + 
Sbjct: 421 -IRNKASAPPEETVLRNKTKVEQTNVLRKEASNSDLNDRKSKADNCSPLKSNLPPSSEI- 480

Query: 481 VCADPEFSDFDTDKGKDCFAVNQVWAIYDTVDGMPRFYARIRKVFSPEFKLQISWFEPHP 540
            C DP+FSDF+ DK + CFAVNQ+WAI+D  D MPRFYA ++KV+ P FKL+I+W EP  
Sbjct: 481 CCPDPDFSDFERDKAEGCFAVNQLWAIFDNTDSMPRFYALVKKVYFP-FKLRITWLEPDS 540

Query: 541 DDKGEIEWCDAELPIACGKYTLGGSELTAELPMFSHMVHCPKQGASKSSYFMYPRKGETW 600
           DD+GEI+W +A LP+ACGK+ LG S+ T++  MFSH VHC K G+   +Y +YP+KGETW
Sbjct: 541 DDQGEIDWHEAGLPVACGKFKLGQSQRTSDRFMFSHQVHCIK-GSDSGTYLVYPKKGETW 600

Query: 601 ALFKDWDIRWSSEPEKHVAFEFEFVEILSDYVEGVGISVAFMDKVKDFVCLFHTTEKHRQ 660
           A+F+ WD+ WSS+PEKH  ++FE+VE+LSD+ E  GI VA++ K+K FV LF  T  +R 
Sbjct: 601 AIFRHWDLGWSSDPEKHSEYQFEYVEVLSDFDENAGIKVAYLSKLKGFVSLFQRTVLNRI 660

Query: 661 NSFKIPPNELYRFSHQIPSVRMTGKERKGVPKGSFELDPAALPPNINDEHVDLNNVKEET 720
           + F I PNELY+FSH+IPS +MTG ER+ VP+GSFELDPA LP          N++ E  
Sbjct: 661 SLFCILPNELYKFSHRIPSYKMTGAERQDVPRGSFELDPAGLP----------NSLSEVG 720

Query: 721 NDAPASSGKTDSSHGFKSPKEKVEVIVLDNNEAAKIQKSNLKKSHPNSEVPTTVRKSPR- 780
           +         + SH  +  K KVE  + +++    I K+ L++S  +  V   +R+SPR 
Sbjct: 721 DPGVVKMDGVNCSH-HEYSKCKVEEAMSNDS----IHKAKLRESIGSERVAQILRRSPRS 780

Query: 781 -KLNLTESDAQVDKFVPEDNRSRDGSRNGLSTHKESSAIHQ-NGGTSTPKKHGESSGLRG 840
            + ++    A   ++    +    G R+       ++A         TP+KH E +   G
Sbjct: 781 SQKSMDNGQANTSQYTVRKDDINIGHRDDSPPEGNTAAFQTIKRKVKTPQKH-EKNNYEG 819

Query: 841 TTCLRIRKSPRDLSKKNA 847
              L+ RKSPRDLSKKNA
Sbjct: 841 -EALKARKSPRDLSKKNA 819

BLAST of Cucsa.163790 vs. TrEMBL
Match: I1JRH7_SOYBN (Uncharacterized protein OS=Glycine max GN=GLYMA_03G242300 PE=4 SV=1)

HSP 1 Score: 94.0 bits (232), Expect = 9.6e-16
Identity = 64/216 (29.63%), Postives = 108/216 (50.00%), Query Frame = 1

Query: 481  FDTDKGKDCFAVNQVWAIYDTVDGMPRFYARIRKV-FSPEFKLQISWFEPHPDDKGEIEW 540
            F  +K ++ F   Q+WAIY   D MP  YA+IR +  +P F+LQ+   EP P        
Sbjct: 858  FKKEKSEEMFQCGQIWAIYGDRDHMPDTYAQIRMIECTPNFRLQVYMLEPCPPPN----- 917

Query: 541  CDAELPIACGKYTLGGSEL-TAELPMFSHMVHCPKQGASKSSYFMYPRKGETWALFKDWD 600
             D +  I+CG +++  ++L    L  FSH +    +  + + Y +YPRK E WAL+KD +
Sbjct: 918  -DLKRTISCGTFSVKEAKLRMLSLSAFSHQLKA--ELVANNRYEIYPRKCEIWALYKDQN 977

Query: 601  IRWSSEPEKHVAFEFEFVEILSDYVEGVGISVAFMDKVKDFVCLFHTTEKHRQNSFKIPP 660
               +S  +     E   VE+L+D  + + + V         +      ++ +    +I  
Sbjct: 978  YELTSSNQGR--GECHIVEVLADSYQSIQVVVLVPHGNSGTIFKAPRIQRSKTGVIEILR 1037

Query: 661  NELYRFSHQIPSVRMTGKERKGVPKGSFELDPAALP 695
             E+ RFSHQIP+ + +        +G +ELDP+++P
Sbjct: 1038 KEVGRFSHQIPAFQHSDNVHL---RGCWELDPSSVP 1060

HSP 2 Score: 603.6 bits (1555), Expect = 3.7e-169
Identity = 353/858 (41.14%), Postives = 488/858 (56.88%), Query Frame = 1

Query: 1   MECNKEEAIKAMKIAETKLEISDFIGARKMAQTAQRLFPTLDNITQLLTVCEIHCSAQNR 60
           MECNK+EAI+A +IAE K++  DF G  K A  AQRLFP + NI Q+L VCE+HC+AQ +
Sbjct: 1   MECNKDEAIRARQIAENKMQAGDFEGGLKFATKAQRLFPEIQNILQILAVCEVHCAAQKK 60

Query: 61  MYGAENDWYGILQIEQSADEAIIKKQYRKLALLLHPDKNKFAGAEAAFKLVGEANRLLSD 120
             G++ DWYGIL+IE+SADEA IKKQYRKLALLLHPDKNK  GAEAAFKL+GEANR+LSD
Sbjct: 61  HSGSDMDWYGILKIEKSADEATIKKQYRKLALLLHPDKNKSDGAEAAFKLIGEANRVLSD 120

Query: 121 QSKRKLYDLKYGAARRNIAPAKSSHDQQNGYTAVNKQERGTANGYSSGPFSHYPGGNSFK 180
           Q+KR LYDLK+G    N A AK      NG  A      GTA  Y +   S Y   NS+ 
Sbjct: 121 QTKRALYDLKFGVPVGNTA-AKVPPRHPNG-NASGMGCDGTARNYQNSFSSQYQAWNSYH 180

Query: 181 PPQPPAQQAFWTCCPFCNVRYQYLKCYLSKMLRCQNCGRGFISHDLNNQTIPPTFHQMHV 240
                  Q FWTCCP CN RYQY+   L+  +RCQ+C + F +HD+ N  + P +     
Sbjct: 181 RTD---NQTFWTCCPHCNTRYQYVITILNHTIRCQHCSKSFTAHDMGNHNVSPGYWSPFN 240

Query: 241 PQKKVAPESGPSKPAAENKQGSVKKSQDR-------SGGVDLNAKAGKKQKGQGSNAKPK 300
            QK+  P+   SK A+++  G     +         S G+  ++K  K++    +    K
Sbjct: 241 NQKE-PPKHASSKEASKSNGGKSSGREQEGVSMSKCSAGIGTHSKVAKRRDSHAAAGVTK 300

Query: 301 ADAEKTGKEKAKSDATSTEKVATKSQNRKRQRKSATAHGNNSEHGDDEVEVDNVSEKDPG 360
           A    +     K    + E  A+     KR R+S +   N + +G        V +    
Sbjct: 301 AGVGMSNPTNTK----AKESQASTKVGHKRARQSTSDDDNKAANG------KGVKDAKVQ 360

Query: 361 LSRDNCQRRSTRNKRQVSYRKYLNEDDDSLQSPNKSSGTASTDLKEEMKDATSNVEASAK 420
            +R +  RRS+R K+ VSY +   + D    S       +S +      D          
Sbjct: 361 KNRVDPNRRSSRKKQHVSYTENDKDGDFGNSSKRPRHHESSNNNPASFTDGVGGQNGK-- 420

Query: 421 GMKQEVLPPHPEDSPNRKPKCEE--VLREGKNGSDKNDNKSKTEIVDTEENGLQGGVHVL 480
            ++ +   P  E     K K E+  VLR+  + SD ND KSK +     ++ L     + 
Sbjct: 421 -IRNKASAPPEETVLRNKTKVEQTNVLRKEASNSDLNDRKSKADNCSPLKSNLPPSSEI- 480

Query: 481 VCADPEFSDFDTDKGKDCFAVNQVWAIYDTVDGMPRFYARIRKVFSPEFKLQISWFEPHP 540
            C DP+FSDF+ DK + CFAVNQ+WAI+D  D MPRFYA ++KV+ P FKL+I+W EP  
Sbjct: 481 CCPDPDFSDFERDKAEGCFAVNQLWAIFDNTDSMPRFYALVKKVYFP-FKLRITWLEPDS 540

Query: 541 DDKGEIEWCDAELPIACGKYTLGGSELTAELPMFSHMVHCPKQGASKSSYFMYPRKGETW 600
           DD+GEI+W +A LP+ACGK+ LG S+ T++  MFSH VHC K G+   +Y +YP+KGETW
Sbjct: 541 DDQGEIDWHEAGLPVACGKFKLGQSQRTSDRFMFSHQVHCIK-GSDSGTYLVYPKKGETW 600

Query: 601 ALFKDWDIRWSSEPEKHVAFEFEFVEILSDYVEGVGISVAFMDKVKDFVCLFHTTEKHRQ 660
           A+F+ WD+ WSS+PEKH  ++FE+VE+LSD+ E  GI VA++ K+K FV LF  T  +R 
Sbjct: 601 AIFRHWDLGWSSDPEKHSEYQFEYVEVLSDFDENAGIKVAYLSKLKGFVSLFQRTVLNRI 660

Query: 661 NSFKIPPNELYRFSHQIPSVRMTGKERKGVPKGSFELDPAALPPNINDEHVDLNNVKEET 720
           + F I PNELY+FSH+IPS +MTG ER+ VP+GSFELDPA LP          N++ E  
Sbjct: 661 SLFCILPNELYKFSHRIPSYKMTGAERQDVPRGSFELDPAGLP----------NSLSEVG 720

Query: 721 NDAPASSGKTDSSHGFKSPKEKVEVIVLDNNEAAKIQKSNLKKSHPNSEVPTTVRKSPR- 780
           +         + SH  +  K KVE  + +++    I K+ L++S  +  V   +R+SPR 
Sbjct: 721 DPGVVKMDGVNCSH-HEYSKCKVEEAMSNDS----IHKAKLRESIGSERVAQILRRSPRS 780

Query: 781 -KLNLTESDAQVDKFVPEDNRSRDGSRNGLSTHKESSAIHQ-NGGTSTPKKHGESSGLRG 840
            + ++    A   ++    +    G R+       ++A         TP+KH E +   G
Sbjct: 781 SQKSMDNGQANTSQYTVRKDDINIGHRDDSPPEGNTAAFQTIKRKVKTPQKH-EKNNYEG 819

Query: 841 TTCLRIRKSPRDLSKKNA 847
              L+ RKSPRDLSKKNA
Sbjct: 841 -EALKARKSPRDLSKKNA 819

BLAST of Cucsa.163790 vs. TrEMBL
Match: A0A0B2P8X1_GLYSO (DnaJ like subfamily B member 14 OS=Glycine soja GN=glysoja_022720 PE=4 SV=1)

HSP 1 Score: 94.0 bits (232), Expect = 9.6e-16
Identity = 64/216 (29.63%), Postives = 108/216 (50.00%), Query Frame = 1

Query: 481  FDTDKGKDCFAVNQVWAIYDTVDGMPRFYARIRKV-FSPEFKLQISWFEPHPDDKGEIEW 540
            F  +K ++ F   Q+WAIY   D MP  YA+IR +  +P F+LQ+   EP P        
Sbjct: 858  FKKEKSEEMFQCGQIWAIYGDRDHMPDTYAQIRMIECTPNFRLQVYMLEPCPPPN----- 917

Query: 541  CDAELPIACGKYTLGGSEL-TAELPMFSHMVHCPKQGASKSSYFMYPRKGETWALFKDWD 600
             D +  I+CG +++  ++L    L  FSH +    +  + + Y +YPRK E WAL+KD +
Sbjct: 918  -DLKRTISCGTFSVKEAKLRMLSLSAFSHQLKA--ELVANNRYEIYPRKCEIWALYKDQN 977

Query: 601  IRWSSEPEKHVAFEFEFVEILSDYVEGVGISVAFMDKVKDFVCLFHTTEKHRQNSFKIPP 660
               +S  +     E   VE+L+D  + + + V         +      ++ +    +I  
Sbjct: 978  YELTSSNQGR--GECHIVEVLADSYQSIQVVVLVPHGNSGTIFKAPRIQRSKTGVIEILR 1037

Query: 661  NELYRFSHQIPSVRMTGKERKGVPKGSFELDPAALP 695
             E+ RFSHQIP+ + +        +G +ELDP+++P
Sbjct: 1038 KEVGRFSHQIPAFQHSDNVHL---RGCWELDPSSVP 1060

HSP 2 Score: 594.0 bits (1530), Expect = 2.9e-166
Identity = 354/868 (40.78%), Postives = 493/868 (56.80%), Query Frame = 1

Query: 1   MECNKEEAIKAMKIAETKLEISDFIGARKMAQTAQRLFPTLDNITQLLTVCEIHCSAQNR 60
           MECNK+EA++A +IAE K++  DF G  K A  AQRLFP + NI Q+L VCE+HC+AQ  
Sbjct: 1   MECNKDEALRARQIAENKMQAGDFEGGLKFATKAQRLFPEIQNILQILAVCEVHCAAQKT 60

Query: 61  MYGAENDWYGILQIEQSADEAIIKKQYRKLALLLHPDKNKFAGAEAAFKLVGEANRLLSD 120
             G++ DWYGIL+ E+SADEA IKKQYRKLALLLHPDKNK AGAEAAFKL+GEANR+LSD
Sbjct: 61  YSGSDMDWYGILKTEKSADEATIKKQYRKLALLLHPDKNKSAGAEAAFKLIGEANRVLSD 120

Query: 121 QSKRKLYDLKYGAARRNIAPAKSSHDQQNGYTAVNKQERGTANGYSSGPFSHYPGGNSFK 180
           Q+KR LYDLK+G    N A  K      NG  A      GTA    +  FS Y   N++ 
Sbjct: 121 QTKRALYDLKFGVPVGNTA-TKVPPRHPNG-NASGMGCDGTARNCQNSYFSQYQAWNAYH 180

Query: 181 PPQPPAQQAFWTCCPFCNVRYQYLKCYLSKMLRCQNCGRGFISHDLNNQTIPPTFHQMHV 240
                  Q FWTCCP CN RYQY+K  L+  +RCQ+C + F +HD+ N  +PP +     
Sbjct: 181 RDD---NQTFWTCCPHCNTRYQYVKTILNHTIRCQHCSKSFTAHDMGNHNVPPGYWAPFN 240

Query: 241 PQKKVAPESGPSKPAAENKQGSVKKSQDR-------SGGVDLNAKAGKKQKGQGSNAKPK 300
            QK+  P+   SK A++   G     +         S G+  ++K  K++ G  +    K
Sbjct: 241 NQKE-PPKHASSKEASKGYGGKSSGREQEGVSMSKCSAGIGAHSKVAKRRDGHVAAGVTK 300

Query: 301 ADAEKTGKEKAKSDATSTEKVATKSQNRKRQRKSATAHGNNSEHGDDEVEVDNVSEKDPG 360
           A    +    +K    + E  A+     KR R+SA+         DD+   +  + KD  
Sbjct: 301 AGVGTSDPTNSK----AKELRASTKVGHKRSRQSAS--------DDDKKAANGKAVKDTK 360

Query: 361 L--SRDNCQRRSTRNKRQVSYRKYLNEDDDSLQSPNKSSGTASTDLKEEMKDATSNVEAS 420
           +  +R +  RRS+R K+ VSY +  N+ D +  + +K      +         T  V   
Sbjct: 361 VQENRVDPNRRSSRKKQHVSYTE--NDKDGNFGNSSKKPRHHKSS-NNNPASFTDGVGGQ 420

Query: 421 AKGMKQEVLPPHPEDSPNRKPKCEE--VLREGKNGSDKNDNKSKTEIVDTEENGLQGGVH 480
              ++ +   P  E     K K E+  V R+  + SD ND KSK +     ++       
Sbjct: 421 NGEIRNKASAPPGETILRNKTKVEQTNVQRKEASNSDLNDRKSKADNCSPLKSNFP-PTS 480

Query: 481 VLVCADPEFSDFDTDKGKDCFAVNQVWAIYDTVDGMPRFYARIRKVFSPEFKLQISWFEP 540
            + C DP+FSDF+ DK +DCFAVNQ+WAI+D  D MPRFYA ++KV+SP FKL+I+W EP
Sbjct: 481 EICCPDPDFSDFERDKAEDCFAVNQLWAIFDNTDSMPRFYALVKKVYSP-FKLRITWLEP 540

Query: 541 HPDDKGEIEWCDAELPIACGKYTLGGSELTAELPMFSHMVHCPKQGASKSSYFMYPRKGE 600
             DD+GEI+W +A LP+ACGK+ LG S+ T++  MFSH +HC K G    +Y +YP+KGE
Sbjct: 541 DSDDQGEIDWHEAGLPVACGKFKLGHSQRTSDRFMFSHQMHCIK-GIDTGTYLIYPKKGE 600

Query: 601 TWALFKDWDIRWSSEPEKHVAFEFEFVEILSDYVEGVGISVAFMDKVKDFVCLFHTTEKH 660
           TWA+F+ WD+ WS +PEKH  ++FE+VE+LSD+ + VG+ VA++ K+K FV LF  T  +
Sbjct: 601 TWAIFRHWDLGWSFDPEKHSEYQFEYVEVLSDFDKNVGVKVAYLSKLKGFVSLFQRTVLN 660

Query: 661 RQNSFKIPPNELYRFSHQIPSVRMTGKERKGVPKGSFELDPAALPPNINDEHVDLNNVKE 720
           R + F I PNELY+FSH IPS +MTG ER+ VP+GSFELDPA LP          N++ E
Sbjct: 661 RISFFCILPNELYKFSHHIPSYKMTGAERQDVPRGSFELDPAGLP----------NSLFE 720

Query: 721 ETNDAPASSGKTDSSHGFKSPKEKVEVIVLDNNEAAKIQKSNLKKSHPNSEVPTTVRKSP 780
             +         + SH  +  K KVE  + +++    I K+ L++S  +  V   +R+SP
Sbjct: 721 VGDPGVVKMDGVNCSH-HEYSKCKVEEAMPNDS----IHKATLRESIDSGRVAQILRRSP 780

Query: 781 R--KLNLTESDAQVDKF---------VPEDNRSRDGSRNGLSTHKESSAIHQNGGTSTPK 840
           R  K ++    A   ++         V  D+   +G+     T K            TP+
Sbjct: 781 RSSKKSMDNGQASTSQYIVRKDDINIVHRDDSPPEGNTAASQTIKRK--------VKTPQ 819

Query: 841 KHGESSGLRGTTCLRIRKSPRDLSKKNA 847
           KH E +   G   L+ RKSP+DL KKNA
Sbjct: 841 KH-EKNNYEG-EALKARKSPKDLGKKNA 819

BLAST of Cucsa.163790 vs. TAIR10
Match: AT5G27240.1 (AT5G27240.1 DNAJ heat shock N-terminal domain-containing protein)

HSP 1 Score: 417.5 bits (1072), Expect = 1.9e-116
Identity = 311/925 (33.62%), Postives = 450/925 (48.65%), Query Frame = 1

Query: 1   MECNKEEAIKAMKIAETKLEISDFIGARKMAQTAQRLFPTLDNITQLLTVCEIHCSAQNR 60
           M+ NKEEA +A  +AE K++  DF+GA+K+   AQ LF  L+++ Q+L VC++H SA+ +
Sbjct: 1   MDWNKEEACRAKTLAEDKMKEGDFVGAQKLLLKAQSLFSGLESLPQMLAVCDVHNSAEKK 60

Query: 61  MYGAENDWYGILQIEQSADEAIIKKQYRKLALLLHPDKNKFAGAEAAFKLVGEANRLLSD 120
           +   EN WYGILQ+   AD+A IKKQ RKLALLLHPDKN+F GAEAAFKLV +A+R L+D
Sbjct: 61  INCLEN-WYGILQVMHFADDATIKKQVRKLALLLHPDKNQFPGAEAAFKLVWDASRFLAD 120

Query: 121 QSKRKLYDLKYGAARRNIAPAKSSHDQQNGYTAVNKQERGTANGYSSGPFSHYPGGNSFK 180
           + KR  YD+     RR I            Y  +   +    +G      +         
Sbjct: 121 KDKRSQYDI-----RRRI------------YLRLATNQLNANSGLQCAATNS-------- 180

Query: 181 PPQPPAQQAFWTCCPFCNVRYQYLKCYLSKMLRCQNCGRGFISHDLNNQTIPPTFHQMHV 240
                A   FWTCC  C  RY+YL+ Y++ +L C  C R ++++D      P    + + 
Sbjct: 181 -----ATDTFWTCCEHCGYRYKYLRKYVNILLNCNICQRSYMAYDTGFNEAPS---KSNT 240

Query: 241 PQKKVAPE---------------SGPSKPAAE-NKQGSV-KKSQDRSGGVDLNAKAGKKQ 300
            QK+V  +               + P   AAE +K+G+  KK   R+GG +      K  
Sbjct: 241 GQKEVQNQGPCNTSLNTNGESIGAQPGSVAAEVDKKGTFNKKFNKRNGGGESKKTEVKNS 300

Query: 301 K--------GQGSNAKPKADAEKTGKEKAKSDATSTEKVATKSQNRKRQRKSATAHGNNS 360
           K              K  A   K   E  + +  +++ V  +S +R  +  S +   N  
Sbjct: 301 KIETEFVRNDDEEKMKSAAKLHKPHPEVTEPEIGASKSVPDESVSRSDEAPSTSKDKNKM 360

Query: 361 EHGDDE-VEVDNVSE-KDPGLSRDNCQRRSTRNKRQVSYRKYLNEDDDSLQSPNKSSGTA 420
           + G +E + VD     KD   S+DN +R+S R  +Q SY +     D+SL  P K    +
Sbjct: 361 KKGVEESINVDGSDFIKDKAYSKDNNKRKSPRRSQQSSYAEEEKISDNSLGPPKKRL-RS 420

Query: 421 STDLKEEMK--------------DATSNVEASAKGMK-----------QEVLPPHPEDSP 480
           +  LK E                D+     A +   K           QE+     ++  
Sbjct: 421 NVGLKSEQTTRKGLVGVGSSKRFDSGGGSSAPSCAFKRKAKKFVDSGYQEISSVKDKERE 480

Query: 481 NRKPKCEEVLREGKNGSDKNDNKSKTEIVDTEENGLQGGVHVLVCADPEFSDFDTDKGKD 540
            RK   E V+ E K  ++ N N++    + TE+             DPEFS+F+      
Sbjct: 481 VRKASGEGVVMEAKIDNNHNPNEN----LITED-----------LPDPEFSNFELTTS-- 540

Query: 541 CFAVNQVWAIYDTVDGMPRFYARIRKVFSPEFKLQISWFEPHPDDKGEIEWCDAELPIAC 600
           CF VNQVW++YD +DGMPR YARI KV  PEFKL I+W +P  D+K      D  +PIAC
Sbjct: 541 CFGVNQVWSMYDPIDGMPRLYARIDKVLVPEFKLWITWIDPLQDNK------DNSIPIAC 600

Query: 601 GKYTLGGSELTAELPMFS-HMVHCPKQGASKSSYFMYPRKGETWALFKDWDIRWSSEPEK 660
           G +  GGSE   +   FS  M H  +     +S  +YPRKGE WA+F+ WDI WS+  E 
Sbjct: 601 GIFQGGGSEEENDHLKFSCQMFHLTRN----NSVVIYPRKGEIWAIFRGWDISWSASSEN 660

Query: 661 HV-AFEFEFVEILSDYVEGVGISVAFMDKVKDFVCLFHTTEKHRQNSFKIPPNELYRFSH 720
           H   +E++FVE+LS++ +  G+ V F+ KV+ FV LF    +      +IPP+++ RFSH
Sbjct: 661 HKHPYEYDFVEVLSNFNDENGLGVGFLGKVEGFVSLFRQDAQDGVLQLQIPPSQMLRFSH 720

Query: 721 QIPSVRMTGKERKGVPKGSFELDPAALPP---NINDEHVDLNNVKE-----------ETN 780
           ++PS +MTGKER+GVP G FELDPAALP     + D  VD+   +E           E +
Sbjct: 721 KVPSFKMTGKEREGVPPGCFELDPAALPKELFEVYDSKVDVGLDRELPNGKTGGPFPEAS 780

Query: 781 DAPASSGKTDSSHGFKSPKEKVEVIVLDNNEAAKIQKSNLKKSHPNSEVPTT-------- 840
                +  T  S  FK  + KV     DN+ +   +    + +H  +    +        
Sbjct: 781 KVEMQANTTRESSPFKKKRPKVH----DNHGSFSKESDGRRTNHERNSAKESRKSVKAVD 840

Query: 841 ---VRKSPRKLNLTESDAQVDKFVPEDNRSRDGSRNGLSTHKESSAIHQNGGTSTPKKHG 844
              +RKSPR L+ T S A               SR+G    ++ SA   N G S  K  G
Sbjct: 841 ALNLRKSPRLLSETNSQA--------------NSRSGRQIAEKKSA---NNGESCGKPGG 842

HSP 2 Score: 95.9 bits (237), Expect = 1.3e-19
Identity = 95/359 (26.46%), Postives = 162/359 (45.13%), Query Frame = 1

Query: 350  KDPGLSRDNCQRRSTRNKRQVSYRKYLNEDDDSLQSPNKSSGTASTDLKEEMKDATSNVE 409
            K P L  +   + ++R+ RQ++ +K  N  +    S  K  G + +  K   K  T    
Sbjct: 780  KSPRLLSETNSQANSRSGRQIAEKKSANNGE----SCGKPGGLSLSGEKMSEKTITR--- 839

Query: 410  ASAKGMKQEVLPPHPEDSPNRKPKCEEVLREGKNGSDKNDNKSKTEIVDTEENGLQGGVH 469
                      +   P+D    KP    V + G+N  D++ ++S+ + + T+ NG      
Sbjct: 840  ----------MRKTPQDI--HKPTVN-VKKHGRN--DESLSQSRGDGLLTQLNGN----- 899

Query: 470  VLVCADPEFS-------------DFDTDKGKDCFAVNQVWAIYDTVDGMPRFYARIRKV- 529
             +  ++PE               +F+  +  D F ++Q+WAIY    G PR YA+I+K+ 
Sbjct: 900  -MKSSEPETRVPSSCKTVKENTFNFENQRSWDKFQIDQIWAIYSNDKGSPRKYAQIKKID 959

Query: 530  FSPEFKLQISWFEPHPDDKGEIEWCDAELPIACGKYTLGGSELTAELPM-FSHMVHCPKQ 589
             SPEFKL ++  E +              P+ CG++ L   +    +P  FSH V   K 
Sbjct: 960  TSPEFKLHVAPLELYRPP------IHMPRPVCCGRFKLKTGKAEVYVPSSFSHQVKAVK- 1019

Query: 590  GASKSSYFMYPRKGETWALFKDWDIRWSSEPEKHVAFEFEFVEILSDYVEGVGISVAFMD 649
               K+ + +YP KGE WAL+K+ + R  +E E     E E VE++    + +  ++    
Sbjct: 1020 -TKKNRFEVYPGKGEIWALYKNCNTRDYTETE-----ELEIVEVVETDEQRIQ-AMTLTA 1079

Query: 650  KVKDFVCLFHTTEKHRQNSFKIPPNELYRFSHQIPSVRMTGKERKGVPKGSFELDPAAL 694
            K  +   L+  +E+   +   IP  E+ RFSHQIP+ R   +  +      +ELD  A+
Sbjct: 1080 KGFNNKPLYRRSEESNASFIDIPKTEVCRFSHQIPAFRHESRATRFGDGQYWELDLKAV 1096

BLAST of Cucsa.163790 vs. TAIR10
Match: AT3G06340.1 (AT3G06340.1 DNAJ heat shock N-terminal domain-containing protein)

HSP 1 Score: 415.6 bits (1067), Expect = 7.3e-116
Identity = 248/729 (34.02%), Postives = 390/729 (53.50%), Query Frame = 1

Query: 1   MECNKEEAIKAMKIAETKLEISDFIGARKMAQTAQRLFPTLDNITQLLTVCEIHCSAQNR 60
           M  N++EA++A  +AE  ++ +DF  ARK+A  AQ++  +L+NI++++ VC++HC+A  +
Sbjct: 1   MSINRDEALRAKDLAEGLMKKTDFTAARKLAMKAQKMDSSLENISRMIMVCDVHCAATEK 60

Query: 61  MYGAENDWYGILQIEQSADEAIIKKQYRKLALLLHPDKNKFAGAEAAFKLVGEANRLLSD 120
           ++G E DWYGILQ+EQ A++ IIKKQY++LALLLHPDKNK  GAE+AFKL+GEA R+L D
Sbjct: 61  LFGTEMDWYGILQVEQIANDVIIKKQYKRLALLLHPDKNKLPGAESAFKLIGEAQRILLD 120

Query: 121 QSKRKLYDLKYGAARRNIAPAKSSHDQQNGYTAVN-KQERGTANGYSS-GPFSHYPGGNS 180
           + KR L+D K    R+  AP   +    N +T  + +    T N ++   P   +P   +
Sbjct: 121 REKRTLHDNKRKTWRKPAAPPYKAQQMPNYHTQPHFRASVNTRNIFTELRPEIRHPFQKA 180

Query: 181 FKPPQPPA---QQAFWTCCPFCNVRYQYLKCYLSKMLRCQNCGRGFISHDLNNQTIP--- 240
               QP A    + F T C FC VRY+Y + +++K + C+ C + F + +   Q+ P   
Sbjct: 181 --QAQPAAFTHLKTFGTSCVFCRVRYEYDRAHVNKEVTCETCKKRFTAFEEPLQSAPQAK 240

Query: 241 -PTFHQMHVPQKKVAPESGPSKPAAENKQGSVKKSQDRSGGVDLNAKAGKKQKGQGSNAK 300
            P+      PQ+   P+        +  +     S  ++                 S   
Sbjct: 241 GPSQTTYCFPQQSKFPDQRACSEPHKRPENPPTVSSSKA-----------------SFPM 300

Query: 301 PKADAEKTGKEKAKSDATSTEKVATKSQNRKRQRKSATAHGNNSEHGDDEVEVDNVSEKD 360
           P + A+  GK K K+ A  +E                ++   +S   +D+V  D  + +D
Sbjct: 301 PGSTAKHNGKRKRKNVAECSE----------------SSDSESSSESEDDVNNDTTAAQD 360

Query: 361 PGLSRDNCQRRSTRNKRQVSYRKYLNEDDDSLQSPNKSSGTASTDLKEEMKDATSNVEAS 420
            G +     RRS R+K++VSY + L++DD  L + N      + D + E           
Sbjct: 361 SGSNGGEQPRRSVRSKQKVSYNENLSDDDVDLVNDNGEGSGKNIDTERE----------- 420

Query: 421 AKGMKQEVLPPHPEDSPNRKPKCEEVLREGKNGSDKNDNKSKTEIVDTEENGLQGGV--- 480
            K  ++E      + +       E +   GK   D+ +  S     D+EE+   G     
Sbjct: 421 -KETEEE------KQTNENHSSTESIDMNGKIEVDQVETPSGAS--DSEEDLSSGSAEKP 480

Query: 481 HVLVCADPEFSDFDTDKGKDCFAVNQVWAIYDTVDGMPRFYARIRKVFSPEFKLQISWFE 540
           +++   DP+F+DFD  + K CF   Q+WA+YD  +GMPRFYA I+KV +P+F L+  WFE
Sbjct: 481 NLINYDDPDFNDFDKLREKSCFQAGQIWAVYDEEEGMPRFYALIKKVTTPDFMLRYVWFE 540

Query: 541 PHPDDKGEIEWCDAELPIACGKYTLGGSELTAELPMFSHMVHCPKQGASKSSYFMYPRKG 600
              D + E       LP++ GK+ +G  E T    +FSH V+   +  ++  + ++P+KG
Sbjct: 541 VDQDQENE----TPNLPVSVGKFVVGNIEETNLCSIFSHFVYSTTKIRTR-KFTVFPKKG 600

Query: 601 ETWALFKDWDIRWSSEPEKHVAFEFEFVEILSDYVEGVGISVAFMDKVKDFVCLFHTTEK 660
           E WALFK+WDI  S++    + +E+EFVEILSD+ EG  +SV F+ KV+ F C+F    K
Sbjct: 601 EIWALFKNWDINCSADSVSPMKYEYEFVEILSDHAEGATVSVGFLSKVQGFNCVFCPMPK 660

Query: 661 HRQNSFKIPPNELYRFSHQIPSVRMTGKERKGVPKGSFELDPAALPPNINDEHVDLNNVK 718
              N+ +IPP+E  RFSH IPS R+TG E +G+ KG +ELDPAALP +++         +
Sbjct: 661 DESNTCEIPPHEFCRFSHSIPSFRLTGTEGRGITKGWYELDPAALPASVSQNLSGEEAAQ 669

BLAST of Cucsa.163790 vs. TAIR10
Match: AT5G18750.1 (AT5G18750.1 DNAJ heat shock N-terminal domain-containing protein)

HSP 1 Score: 392.9 bits (1008), Expect = 5.0e-109
Identity = 268/752 (35.64%), Postives = 379/752 (50.40%), Query Frame = 1

Query: 4   NKEEAIKAMKIAETKLEISDFIGARKMAQTAQRLFPTLDNIT-QLLTVCEIHCSAQNRMY 63
           NK+EA++A  +AE  +  SDF  AR++A  AQ++  TL+++  +++ VC++HC+A  +  
Sbjct: 3   NKDEALRAKDLAEDWMSKSDFTTARRIAIKAQKMDATLESVVARMIMVCDVHCAALEKS- 62

Query: 64  GAENDWYGILQIEQSADEAIIKKQYRKLALLLHPDKNKFAGAEAAFKLVGEANRLLSDQS 123
           G E DWY ILQ+EQ+ADE  IKKQY+KLAL LHPDKNK  GAE+AFK +GEA R+L D+ 
Sbjct: 63  GDETDWYKILQVEQTADENTIKKQYKKLALHLHPDKNKLPGAESAFKTIGEAQRVLLDKD 122

Query: 124 KRKLYDLKYGAARRNIAPAK----SSHDQQNGYTAVNKQERGTANGYSSGPFSHYPGGNS 183
           KR+ +D++     R  APA     S    Q   T     +RG     +    +     N 
Sbjct: 123 KRRFHDMRRKPVFRRPAPAPAPAPSFQPPQQAPTTPFFTQRGFQTNVN---VARKRPENQ 182

Query: 184 FKPPQPPAQ----QAFWTCCPFCNVRYQYLKCYLSKMLRCQNCGRGFISHDLNNQTIPPT 243
            KP   P       +F T C FC+ +Y+Y +  ++ ++ C NCG+ +++     +T  P 
Sbjct: 183 KKPQAQPTGFDGLASFTTSCAFCHRKYEYQRKLINTLMTCLNCGKQYVAFQ---ETFQP- 242

Query: 244 FHQMHVPQKKVAPESGPSKPAAENKQGSVKKSQDRSGGVDLNAKAGKKQKGQGSNAKPKA 303
                           P +P     Q S   +Q+          AGK  + Q  +   K+
Sbjct: 243 ----------------PVQPTFSFFQQSKVPTQE----------AGKAVEKQPESCA-KS 302

Query: 304 DAEKTGKEKAKSDATSTEKVATKSQNRKRQRKSATAHGNNSEHGDDEVEVDNVSEKDPGL 363
              K G  +AKS   S E +  K   RKR++   ++  + SE   D  EV     +D G 
Sbjct: 303 FFSKEGS-RAKSSGVSAENINGK---RKRKKVVESSDSSCSESSIDCNEVP-AGGQDSGS 362

Query: 364 SRDNCQRRSTRNKRQVSYRKYLNEDDDSLQSPNKSSGTASTDLKEEMKDATSNVEASAKG 423
           S     RRS R+K+QVSY                         KE+ ++   + E S   
Sbjct: 363 SGAQHSRRSVRSKQQVSY-------------------------KEDKEEDAESAEESDFR 422

Query: 424 MKQEVLPPHPEDSPNRKPKCEEVLREGKNGSDKNDNKSKTEIVDTEENGLQGGVHVLVCA 483
            K     P  E  PN       + R  K+ + KN +       + +            C 
Sbjct: 423 KKSHEDQPFTETLPNG------LNRTKKSKAAKNSSSGSASDAEIQ------------CT 482

Query: 484 DPEFSDFDTDKGKDCFAVNQVWAIYDTVDGMPRFYARIRKVF-SPEFKLQISWFEPHPDD 543
           DP+FS+F+  +   CF   Q WAIYD + GMPR+YA IRKV   P F L+I W E  PDD
Sbjct: 483 DPDFSNFEKSREVTCFKAGQTWAIYDDMGGMPRYYAIIRKVIRKPSFMLKIQWLEAEPDD 542

Query: 544 KGEIEWCDAELPIACGKYTLGGSELTAELPMFSHMVHCPKQGASKSSYFMYPRKGETWAL 603
           +    W    LPI+ GK+ LGG+E   + P FSHM++  K G+ K +  +YPR GETWAL
Sbjct: 543 EKANLWVRKNLPISIGKFKLGGNENIEKTPCFSHMIYF-KVGSMKDTVRVYPRIGETWAL 602

Query: 604 FKDWDIRWSSEPEKHV---AFEFEFVEILSDYVEGVGISVAFMDKVKDFVCLF-HTTEKH 663
           FK+WDI WSS   +      +E+EFVEILS+YVEGV I VAF+ K+K F  +F       
Sbjct: 603 FKNWDINWSSGRRRSSHEHEYEYEFVEILSEYVEGVAIQVAFLRKIKGFTSVFCRIAPGG 661

Query: 664 RQNSFKIPPNELYRFSHQIPSVRMTGKERKGVPKGSFELDPAALPPNINDEHVDLNNVKE 723
             ++ +IPP+EL RFSH IPS ++TGKE  GVP GS+E D AALP  I          +E
Sbjct: 663 GSDTIQIPPHELLRFSHSIPSTKLTGKEGNGVPIGSYEFDTAALPQKIE---------QE 661

Query: 724 ETNDAPASSGKTDSSHGFKSPKEKVEVIVLDN 742
           E       S K +  +    P  + + IV+ N
Sbjct: 723 EAVPVLRESAKLNQVNHNSPPSSEPDCIVIPN 661

HSP 2 Score: 133.7 bits (335), Expect = 5.5e-31
Identity = 81/231 (35.06%), Postives = 128/231 (55.41%), Query Frame = 1

Query: 471 LVCADPEFSDFDTDKGKDCFAVNQVWAIYDTVDGMPRFYARIRK-VFSPEFKLQISWFEP 530
           +V  + +F++F  ++ +  FA  Q+W++    DG+P+ YA+I++ V+ P FKLQI+  EP
Sbjct: 657 IVIPNFQFNNFSAERLEGKFAPGQIWSLNSKEDGLPKCYAKIQQIVWRPVFKLQINRLEP 716

Query: 531 HPDDKGEIEWCDAELPIACGKYTL--GGSELTAELPMFSHMVHCPKQGASKSSYFMYPRK 590
               +  I+W D  +P++CG +TL  G  E   ++  FSH +   K     + Y + P+ 
Sbjct: 717 KSLLENVIQWHDKRMPVSCGNFTLKEGRDETLTKVTDFSHQIKAEKH-FRINEYIVVPKT 776

Query: 591 GETWALFKDWDIRWSSEPEKHVAFEFEFVEILSDYVEGVGISVAFMDKVKDFVCLF-HTT 650
           GE WA++K+W     +   K    E+E VE+L D      I V  +++V  F+ +F    
Sbjct: 777 GEIWAMYKNWSETIKATSLKRC--EYEVVEVLDD--NDSHIEVMLLEQVDGFISVFKEKL 836

Query: 651 EKHRQNSFKIPPNELYRFSHQIPSVRMTGKERKGVPKGSFELDPAALPPNI 698
           E       KIP  EL RFSH +P+ R+TG ER G  +G  ELDP+A P N+
Sbjct: 837 EGGIDVKKKIPRCELLRFSHYVPAFRLTG-ERDGALRGYVELDPSAFPLNL 881

BLAST of Cucsa.163790 vs. TAIR10
Match: AT5G53150.1 (AT5G53150.1 DNAJ heat shock N-terminal domain-containing protein)

HSP 1 Score: 298.1 bits (762), Expect = 1.7e-80
Identity = 237/789 (30.04%), Postives = 350/789 (44.36%), Query Frame = 1

Query: 1   MECNKEEAIKAMKIAETKLEISDFIGARKMAQTAQRLFPTLDNITQLLTVCEIHCSAQNR 60
           MECNK+EA +AM IAE K+   D+ GA+K A  AQ LFP LD + QL     ++ S + +
Sbjct: 1   MECNKDEAKRAMDIAERKMTEKDYTGAKKFANKAQNLFPELDGLKQLFVAINVYISGE-K 60

Query: 61  MYGAENDWYGILQIEQSADEAIIKKQYRKLALLLHPDKNKFAGAEAAFKLVGEANRLLSD 120
            +  E DWYG+L ++  A +  +KKQYRKL L+LHPDKNK  GAE AF LV EA  LLSD
Sbjct: 61  TFAGEADWYGVLGVDPFASDEALKKQYRKLVLMLHPDKNKCKGAEGAFNLVAEAWALLSD 120

Query: 121 QSKRKLYDLKYG----AARRNIAPAK----SSHDQQNGYTAVNKQERGTANGYSSGPFSH 180
           + KR LY++K G    AA++     +    S     NG   V +    +A      P + 
Sbjct: 121 KDKRILYNVKRGKDVKAAQQRFPTTQREIPSHQPTSNGIPNVREHVVSSARARYK-PATR 180

Query: 181 YP---------GGNSFKPPQPPAQQAFWTCCPFCNVRYQYLKCYLSKMLRCQNCGRGFIS 240
            P         G  +F  P   +   FWT C  C+ +Y+Y + YL               
Sbjct: 181 KPAARMDRSRTGSPAFVYPTQESS-TFWTMCNKCDTQYEYQRVYL--------------- 240

Query: 241 HDLNNQTIPPTFHQMHVPQKKVAPESGPSKPAAENKQGSVKKSQDRSGGVDLNAKAGKKQ 300
               NQT+             + P       A E      K          +N  + +  
Sbjct: 241 ----NQTL-------------LCPHCHHGFVAEE------KTPPTNIPKPPVNISSNQHH 300

Query: 301 KGQGSNAKPKADAEKTGKEKAKSDATSTEKVATKSQNRKRQRKSATAHGNNSEHGDDEVE 360
           +   + A  K            S+ +S  +    S N   Q  S+   G+ S +  +E  
Sbjct: 301 RSSKNQASNK-----------NSNGSSYRREPATSVNHNFQWDSSRMGGSYSRNATNETA 360

Query: 361 --VDNVSEKDPGLSRDNCQRRSTRN--KRQVSYRKYLNEDDDSLQSPNKSS------GTA 420
             V    +K   +  +  +R + R      +   K    DD  ++ P+  S         
Sbjct: 361 NVVQQGQDKLKRVFWETQEREAARGFTNSDLGNFKRQKTDDSHMRGPSAGSRHPYVQALL 420

Query: 421 STDLKEEMKDATSN-----VEASAKGMKQEVLPPHPEDSPNR--KPKCEEVLREGKNGSD 480
            +D+K+ + D   +     +      M+ +V P   E +  +    K  EV R  K  S 
Sbjct: 421 RSDIKKALMDRGQSEIFKRLPMMIAKMEGKVNPTEGEKNSTKAMSSKASEVERS-KMSST 480

Query: 481 KNDNKSKTEIVDTEENGLQGGVHVLVCADPEFSDFDTDKGKDCFAVNQVWAIYDTVDGMP 540
            N+ +   E++  E +     V  +V  D +F +FD D+ +  F  +Q+WA YD  DGMP
Sbjct: 481 ANEVERSVEVIPHESDE----VKEIVVPDSDFHNFDLDRSESAFKDDQIWAAYDDADGMP 540

Query: 541 RFYARIRKVFSPE-FKLQISWFEPHPDDK-GEIEWCDAELPIACGKYTLGGSELTAELPM 600
           RFYARI+KV S   FKL+ISW       + G I+W  A    +CG +  G  E T  L  
Sbjct: 541 RFYARIQKVISVNPFKLKISWLNSKTTSEFGPIDWMGAGFAKSCGDFRCGRYESTDTLNA 600

Query: 601 FSHMVHCPKQGASKSSYFMYPRKGETWALFKDWDIRWSSEPEKHVAFEFEFVEILSDYVE 660
           FSH V   K   ++    + P+KG+ WAL+++W   W       V  ++E VE+L DY E
Sbjct: 601 FSHSVDFTK--GARGLLHILPKKGQVWALYRNWSPEWDKNTPDEVKHKYEMVEVLDDYTE 660

Query: 661 -GVGISVAFMDKVKDFVCLF-HTTEKHRQNSFKIPPNELYRFSHQIPSVRMTGKERKGVP 720
               ++VA + K + F  +F   TEK      KI   E+ RFSHQ+P   +TGKE    P
Sbjct: 661 DDQSLTVALLLKAEGFRVVFRRCTEK--LGVRKIAKEEMLRFSHQVPHYILTGKEADNAP 718

Query: 721 KGSFELDPAALPPNINDEHVDLNNVKEETNDAPASSGKTDSSHGFKSPKEKVEVIVLDNN 752
           +G  ELDPAA P   + E+ + +   E   +          +   ++ KE  E   L  N
Sbjct: 721 EGFLELDPAATPCAFSSENAEADEKSEAVKE----------NEQGEAVKENEESEALKEN 718

BLAST of Cucsa.163790 vs. TAIR10
Match: AT5G18740.1 (AT5G18740.1 Domain of unknown function (DUF3444))

HSP 1 Score: 220.7 bits (561), Expect = 3.4e-57
Identity = 133/418 (31.82%), Postives = 209/418 (50.00%), Query Frame = 1

Query: 307 DATSTEKVATKSQNRKRQRKSATAHGNNSEHGDDE-----VEVDNVSEKDPGLSRDNCQR 366
           D T  E +  K      Q KS       + H +       ++  +V  K PG    NC  
Sbjct: 3   DDTVNELLRRKKAVLNHQIKSDLVASTLANHQEKPQAFSLLQQKDVQTKSPGTHFSNCAV 62

Query: 367 RSTRNKRQVSYRKYLNEDDDSLQSP--NKSSGTASTDLKEEMKDATSNVEASAKGMKQEV 426
           +    +++       N ++ S+     N S        +    D     +    G +   
Sbjct: 63  KVGEKRKRNECGGICNTENRSMTEDVVNLSKDRNVDSKRSAFSDNGDATQEFGSGKQL-- 122

Query: 427 LPPHPEDSPNRKPKCEEVLREGKNGSDKNDNKSKTEIVDTEENGLQGGVHVLVCADPEFS 486
               PE   ++ P     +      S   +    + + D+E  G+     +   A  +F+
Sbjct: 123 ----PEVDCSKDPMSNAQVGAVVGNSRNLEVDQNSGLCDSESGGVVPQ-KISGFAGLKFN 182

Query: 487 DFDTDKGKDCFAVNQVWAIYDTVDGMPRFYARIRKVFSPEFKLQISWFEPHPDDKGEIEW 546
           DFD  + +  F V Q WAI+D VDGMPR YA+I KV +P F L+I++ EP PD + E++W
Sbjct: 183 DFDKLREEVNFEVGQTWAIFDPVDGMPRLYAKIIKVSAPCFGLRITYLEPDPDGEKELQW 242

Query: 547 CDAELPIACGKYTLGGSELTAELPMFSHMVHCPKQG-----------ASKSSYFMYPRKG 606
            + +LP++ G + LG ++ T +  +FSH++HC +              +   + + PR+G
Sbjct: 243 FEEDLPVSVGNFRLGENKCTQDRSIFSHVIHCNELSNTLCFSVTCRFINTCHFSVSPREG 302

Query: 607 ETWALFKDWDIRWSSEPEKHVAFEFEFVEILSDYVEGVGISVAFMDKVKDFVCLFHTTEK 666
           ETWALFK+WDI+WSSEP+ H  +E+EFVEILSDY +  G+ VA++ K K F  +F     
Sbjct: 303 ETWALFKNWDIKWSSEPDSHRKYEYEFVEILSDYADEAGVYVAYLHKAKGFASVFLRMGT 362

Query: 667 HRQNSFKIPPNELYRFSHQIPSVRMTGKERKGVPKGSFELDPAALPPNINDEHVDLNN 707
             +  F+I P  LYRFSH++PS ++TG + K +PK ++ELD AALP  I +  V  N+
Sbjct: 363 GYEGIFRILPRSLYRFSHRVPSFKLTGVKGKDMPKDAYELDQAALPETIEEIIVPSNS 413

BLAST of Cucsa.163790 vs. NCBI nr
Match: gi|778662617|ref|XP_011659923.1| (PREDICTED: uncharacterized protein LOC101213491 [Cucumis sativus])

HSP 1 Score: 1734.5 bits (4491), Expect = 0.0e+00
Identity = 846/847 (99.88%), Postives = 847/847 (100.00%), Query Frame = 1

Query: 1   MECNKEEAIKAMKIAETKLEISDFIGARKMAQTAQRLFPTLDNITQLLTVCEIHCSAQNR 60
           MECNKEEAIKAMKIAETKLEISDFIGARKMAQTAQRLFPTLDNITQLLTVCEIHCSAQNR
Sbjct: 1   MECNKEEAIKAMKIAETKLEISDFIGARKMAQTAQRLFPTLDNITQLLTVCEIHCSAQNR 60

Query: 61  MYGAENDWYGILQIEQSADEAIIKKQYRKLALLLHPDKNKFAGAEAAFKLVGEANRLLSD 120
           MYGAENDWYGILQIEQSADEAIIKKQYRKLALLLHPDKNKFAGAEAAFKLVGEANRLLSD
Sbjct: 61  MYGAENDWYGILQIEQSADEAIIKKQYRKLALLLHPDKNKFAGAEAAFKLVGEANRLLSD 120

Query: 121 QSKRKLYDLKYGAARRNIAPAKSSHDQQNGYTAVNKQERGTANGYSSGPFSHYPGGNSFK 180
           QSKRKLYDLKYGAARRNIAPAKSSHDQQNGYTAVNKQERGTANGYSSGPFSHYPGGNSFK
Sbjct: 121 QSKRKLYDLKYGAARRNIAPAKSSHDQQNGYTAVNKQERGTANGYSSGPFSHYPGGNSFK 180

Query: 181 PPQPPAQQAFWTCCPFCNVRYQYLKCYLSKMLRCQNCGRGFISHDLNNQTIPPTFHQMHV 240
           PPQPPAQQAFWTCCPFCNVRYQYLKCYLSKMLRCQNCGRGFISHDLNNQTIPPTFHQM+V
Sbjct: 181 PPQPPAQQAFWTCCPFCNVRYQYLKCYLSKMLRCQNCGRGFISHDLNNQTIPPTFHQMNV 240

Query: 241 PQKKVAPESGPSKPAAENKQGSVKKSQDRSGGVDLNAKAGKKQKGQGSNAKPKADAEKTG 300
           PQKKVAPESGPSKPAAENKQGSVKKSQDRSGGVDLNAKAGKKQKGQGSNAKPKADAEKTG
Sbjct: 241 PQKKVAPESGPSKPAAENKQGSVKKSQDRSGGVDLNAKAGKKQKGQGSNAKPKADAEKTG 300

Query: 301 KEKAKSDATSTEKVATKSQNRKRQRKSATAHGNNSEHGDDEVEVDNVSEKDPGLSRDNCQ 360
           KEKAKSDATSTEKVATKSQNRKRQRKSATAHGNNSEHGDDEVEVDNVSEKDPGLSRDNCQ
Sbjct: 301 KEKAKSDATSTEKVATKSQNRKRQRKSATAHGNNSEHGDDEVEVDNVSEKDPGLSRDNCQ 360

Query: 361 RRSTRNKRQVSYRKYLNEDDDSLQSPNKSSGTASTDLKEEMKDATSNVEASAKGMKQEVL 420
           RRSTRNKRQVSYRKYLNEDDDSLQSPNKSSGTASTDLKEEMKDATSNVEASAKGMKQEVL
Sbjct: 361 RRSTRNKRQVSYRKYLNEDDDSLQSPNKSSGTASTDLKEEMKDATSNVEASAKGMKQEVL 420

Query: 421 PPHPEDSPNRKPKCEEVLREGKNGSDKNDNKSKTEIVDTEENGLQGGVHVLVCADPEFSD 480
           PPHPEDSPNRKPKCEEVLREGKNGSDKNDNKSKTEIVDTEENGLQGGVHVLVCADPEFSD
Sbjct: 421 PPHPEDSPNRKPKCEEVLREGKNGSDKNDNKSKTEIVDTEENGLQGGVHVLVCADPEFSD 480

Query: 481 FDTDKGKDCFAVNQVWAIYDTVDGMPRFYARIRKVFSPEFKLQISWFEPHPDDKGEIEWC 540
           FDTDKGKDCFAVNQVWAIYDTVDGMPRFYARIRKVFSPEFKLQISWFEPHPDDKGEIEWC
Sbjct: 481 FDTDKGKDCFAVNQVWAIYDTVDGMPRFYARIRKVFSPEFKLQISWFEPHPDDKGEIEWC 540

Query: 541 DAELPIACGKYTLGGSELTAELPMFSHMVHCPKQGASKSSYFMYPRKGETWALFKDWDIR 600
           DAELPIACGKYTLGGSELTAELPMFSHMVHCPKQGASKSSYFMYPRKGETWALFKDWDIR
Sbjct: 541 DAELPIACGKYTLGGSELTAELPMFSHMVHCPKQGASKSSYFMYPRKGETWALFKDWDIR 600

Query: 601 WSSEPEKHVAFEFEFVEILSDYVEGVGISVAFMDKVKDFVCLFHTTEKHRQNSFKIPPNE 660
           WSSEPEKHVAFEFEFVEILSDYVEGVGISVAFMDKVKDFVCLFHTTEKHRQNSFKIPPNE
Sbjct: 601 WSSEPEKHVAFEFEFVEILSDYVEGVGISVAFMDKVKDFVCLFHTTEKHRQNSFKIPPNE 660

Query: 661 LYRFSHQIPSVRMTGKERKGVPKGSFELDPAALPPNINDEHVDLNNVKEETNDAPASSGK 720
           LYRFSHQIPSVRMTGKERKGVPKGSFELDPAALPPNINDEHVDLNNVKEETNDAPASSGK
Sbjct: 661 LYRFSHQIPSVRMTGKERKGVPKGSFELDPAALPPNINDEHVDLNNVKEETNDAPASSGK 720

Query: 721 TDSSHGFKSPKEKVEVIVLDNNEAAKIQKSNLKKSHPNSEVPTTVRKSPRKLNLTESDAQ 780
           TDSSHGFKSPKEKVEVIVLDNNEAAKIQKSNLKKSHPNSEVPTTVRKSPRKLNLTESDAQ
Sbjct: 721 TDSSHGFKSPKEKVEVIVLDNNEAAKIQKSNLKKSHPNSEVPTTVRKSPRKLNLTESDAQ 780

Query: 781 VDKFVPEDNRSRDGSRNGLSTHKESSAIHQNGGTSTPKKHGESSGLRGTTCLRIRKSPRD 840
           VDKFVPEDNRSRDGSRNGLSTHKESSAIHQNGGTSTPKKHGESSGLRGTTCLRIRKSPRD
Sbjct: 781 VDKFVPEDNRSRDGSRNGLSTHKESSAIHQNGGTSTPKKHGESSGLRGTTCLRIRKSPRD 840

Query: 841 LSKKNAG 848
           LSKKNAG
Sbjct: 841 LSKKNAG 847

BLAST of Cucsa.163790 vs. NCBI nr
Match: gi|659099671|ref|XP_008450718.1| (PREDICTED: uncharacterized protein LOC103492216 [Cucumis melo])

HSP 1 Score: 1577.4 bits (4083), Expect = 0.0e+00
Identity = 782/850 (92.00%), Postives = 805/850 (94.71%), Query Frame = 1

Query: 1   MECNKEEAIKAMKIAETKLEISDFIGARKMAQTAQRLFPTLDNITQLLTVCEIHCSAQNR 60
           MECNKEEAIKAMKIAE KLEISDF GARKMAQTAQRLFPTLDNITQLLTVCEIHCSAQNR
Sbjct: 1   MECNKEEAIKAMKIAEKKLEISDFNGARKMAQTAQRLFPTLDNITQLLTVCEIHCSAQNR 60

Query: 61  MYGAENDWYGILQIEQSADEAIIKKQYRKLALLLHPDKNKFAGAEAAFKLVGEANRLLSD 120
           MYGAENDWYGILQIEQSADE IIKKQYRKLALLLHPDKNKFAGAEAAFKLVGEANRLLSD
Sbjct: 61  MYGAENDWYGILQIEQSADETIIKKQYRKLALLLHPDKNKFAGAEAAFKLVGEANRLLSD 120

Query: 121 QSKRKLYDLKYGAARRNIAPAKSSHDQQNGYTAVNKQERGTANGYSSGPFSHYPGGNSFK 180
           QSKRKLYDLKY AARRNIA AKSSHDQQNGYTAVNKQERGTANGYSSG FSHYPGG+SFK
Sbjct: 121 QSKRKLYDLKYAAARRNIAAAKSSHDQQNGYTAVNKQERGTANGYSSGSFSHYPGGSSFK 180

Query: 181 PPQPPAQQAFWTCCPFCNVRYQYLKCYLSKMLRCQNCGRGFISHDLNNQTIPPTFHQMHV 240
           PPQPP QQAFWTCCPFCNVRYQYLKCYLSKMLRCQNCGRGFISHDLNNQT+PPTFHQMHV
Sbjct: 181 PPQPPPQQAFWTCCPFCNVRYQYLKCYLSKMLRCQNCGRGFISHDLNNQTMPPTFHQMHV 240

Query: 241 PQKKVAPESGPSKPAAENKQGSVKKSQDRSGGVDLNAKAGKKQKGQGSNAKPKADAEKTG 300
           PQKKVAPESGPSKPAAENKQGS KKSQDRS GVDLNAKAGKKQKGQGSNAKPK DAEKTG
Sbjct: 241 PQKKVAPESGPSKPAAENKQGSDKKSQDRSAGVDLNAKAGKKQKGQGSNAKPKVDAEKTG 300

Query: 301 KEKAKSDAT-STEKVATKSQNRKRQRKSATAHGNNSEHGDDEVEVDNVSEKDPGLSRDNC 360
           KEKAKSDA  STEKVA+KSQNRKRQRKSATAHGNN E+  DEVE DNVSEKD GLSRDN 
Sbjct: 301 KEKAKSDAARSTEKVASKSQNRKRQRKSATAHGNNLEY--DEVEDDNVSEKD-GLSRDNS 360

Query: 361 QRRSTRNKRQVSYRKYLNEDDDSLQSPNKSSGTASTDLKEEMKDATSNVEASAKGMKQEV 420
           QRRSTRNK+ VSYRKYL+EDDDSLQSP KSSGTASTDLKEEMKDA  NVE SAKG+KQEV
Sbjct: 361 QRRSTRNKKHVSYRKYLSEDDDSLQSPKKSSGTASTDLKEEMKDAILNVETSAKGIKQEV 420

Query: 421 LPPHPEDSPNRKPKCEEVLREGKNGSDKNDNKSKTEIVDTEENGLQGGVHVLVCADPEFS 480
           LPPHP+DSPNRKPKCEEV+REGKN SDKND KSKTE+VDTEENG+Q  VHVLVCADPEFS
Sbjct: 421 LPPHPQDSPNRKPKCEEVVREGKNRSDKNDKKSKTEVVDTEENGVQ-AVHVLVCADPEFS 480

Query: 481 DFDTDKGKDCFAVNQVWAIYDTVDGMPRFYARIRKVFSPEFKLQISWFEPHPDDKGEIEW 540
           DFD DKG DCFAVNQVWAIYDTVDGMPRFYARIRKVFSPEFKLQI+WFEPHPDDK EI+W
Sbjct: 481 DFDKDKGTDCFAVNQVWAIYDTVDGMPRFYARIRKVFSPEFKLQITWFEPHPDDKDEIKW 540

Query: 541 CDAELPIACGKYTLGGSELTAELPMFSHMVHCPKQGASKSSYFMYPRKGETWALFKDWDI 600
           CDAELPIACGKYTLGGSELTA+LPMFSHMVHCPKQGASK+SYFMYPRKGETWALFKDWDI
Sbjct: 541 CDAELPIACGKYTLGGSELTADLPMFSHMVHCPKQGASKNSYFMYPRKGETWALFKDWDI 600

Query: 601 RWSSEPEKHVAFEFEFVEILSDYVEGVGISVAFMDKVKDFVCLFHTTEKHRQNSFKIPPN 660
           RWS EPEKHVAFE+EFVEILSDYVEGVGISVAFMDKVK FVCLFHTTEKHRQNSFKIPPN
Sbjct: 601 RWSFEPEKHVAFEYEFVEILSDYVEGVGISVAFMDKVKGFVCLFHTTEKHRQNSFKIPPN 660

Query: 661 ELYRFSHQIPSVRMTGKERKGVPKGSFELDPAALPPNINDEHVDLNNVKEETNDAPASSG 720
           ELYRFSHQIPSVRMTGKERK VPKGSFELDPAALPPNIN EHVDLNNVKEET DAPA+SG
Sbjct: 661 ELYRFSHQIPSVRMTGKERKDVPKGSFELDPAALPPNIN-EHVDLNNVKEETIDAPAASG 720

Query: 721 KTDSSHGFKSPKEKVEVIVLDNNEAAKIQKSNLKKSHPNSEVPTTVRKSPRKL--NLTES 780
           +TD SHGFKSPKEKVEVI LDNNEAAKIQKS+LKKSHPN EVPTT+RKSPRKL  N TE+
Sbjct: 721 RTDPSHGFKSPKEKVEVIDLDNNEAAKIQKSDLKKSHPNCEVPTTLRKSPRKLNPNPTEN 780

Query: 781 DAQVDKFVPEDNRSRDGSRNGLSTHKESSAIHQNGGTSTPKKHGESSGLRGTTCLRIRKS 840
           DAQ+DKF PEDNRSRDGSRNGLST KESS IHQNGGT+TPKKHGESSGLRGTTCL IRKS
Sbjct: 781 DAQLDKFAPEDNRSRDGSRNGLSTRKESSTIHQNGGTNTPKKHGESSGLRGTTCLGIRKS 840

Query: 841 PRDLSKKNAG 848
           PRDLSKKNAG
Sbjct: 841 PRDLSKKNAG 845

BLAST of Cucsa.163790 vs. NCBI nr
Match: gi|359487946|ref|XP_002266568.2| (PREDICTED: uncharacterized protein LOC100255461 [Vitis vinifera])

HSP 1 Score: 674.1 bits (1738), Expect = 3.2e-190
Identity = 401/901 (44.51%), Postives = 544/901 (60.38%), Query Frame = 1

Query: 1   MECNKEEAIKAMKIAETKLEISDFIGARKMAQTAQRLFPTLDNITQLLTVCEIHCSAQNR 60
           M+CNKEEA++A  I+E K++  DFIGAR++AQ AQ+LFP L+NI+QLLTVC++HCSAQN+
Sbjct: 1   MDCNKEEALRAKVISEKKMQSGDFIGARRIAQRAQQLFPDLENISQLLTVCDVHCSAQNK 60

Query: 61  MYGAENDWYGILQIEQSADEAIIKKQYRKLALLLHPDKNKFAGAEAAFKLVGEANRLLSD 120
           +YG E DWYGIL++EQ+AD+AIIKKQYRKLALLLHPDKNKFAGAEAAFKL+GEANR+LSD
Sbjct: 61  IYGTEMDWYGILKVEQAADDAIIKKQYRKLALLLHPDKNKFAGAEAAFKLIGEANRILSD 120

Query: 121 QSKRKLYDLKYGAARRNIAPAKSSHDQQNGYTAVNKQERGTANGYSSGPFSHYPGGNSFK 180
           Q KR  YD+KY  + ++ AP    H Q N  + V KQ  G  N + +    H  G N  +
Sbjct: 121 QGKRSAYDMKYRVSLKHTAPKPPPH-QLNRNSFVRKQ-YGVQNNFPNVANPHGVGLNPHQ 180

Query: 181 PPQP---PAQQAFWTCCPFCNVRYQYLKCYLSKMLRCQNCGRGFISHDLNNQTIPP--TF 240
             QP     QQ FWTCCPFC++RYQY +  ++++LRCQ C + FI++DL  Q++PP  T+
Sbjct: 181 QTQPGLSDGQQTFWTCCPFCSIRYQYYRDIMNRVLRCQTCQKSFIAYDLGAQSVPPGATW 240

Query: 241 HQ----MH--VPQK---KVAPESGPSKPAAENKQGSVKKSQDRSGGVDLNAK---AGKKQ 300
            Q    +H  VP +   KV  +S    P +   QGS      ++ G DL  K   A +  
Sbjct: 241 SQPAFSLHNEVPNQCPIKVKTQSPAMNPGSMGSQGSF---NSKTAGPDLVKKKRCADEAI 300

Query: 301 KGQGSNAKPKADAE---KTGKEKAKSDATSTEKVATKSQNRKRQRKSATAHGNNS---EH 360
            G  +N K   + +   K G    KSDA    K  +  +N  R+RK+     + S     
Sbjct: 301 GGSKTNGKEDGNVDVGSKKGVRMPKSDADKPRKSGSSRRNTSRKRKNLPVESSESCQTSS 360

Query: 361 GDDEVEVDNVSEKDPGLSRDNCQ-------RRSTRNKRQVSYRKYLNEDDDSLQSPNKSS 420
            +D  E     EK    S +N +       RRS+R K+ V Y + +++DDD +  P K+ 
Sbjct: 361 SEDAKEAAIAQEKGVVPSGENSEFNIGHQPRRSSRKKQHVYYNESVSDDDDFVSPPKKAR 420

Query: 421 GTASTDLKEEMKDA---------------TSNVEASAKGMKQEVLPPHPEDSPNRKPKCE 480
              S    EE KD                TS V+   + +KQ+   P  E    RK +  
Sbjct: 421 MDGSLGTGEERKDKPLDDGVPKTCNTAGFTSVVDVGKENIKQKENVPLEETVVKRKSEAG 480

Query: 481 EVLREGKNGSDKNDNKSKTE-IVDTEENGLQGGVH---VLVCADPEFSDFDTDKGKDCFA 540
             +  GK  +  +DN  + +  V++E N      H    L C D +FSDFD DK +DCF+
Sbjct: 481 GCMINGKAAATADDNDERCKGSVNSEPNSCPDVTHEPVSLECLDCDFSDFDKDKREDCFS 540

Query: 541 VNQVWAIYDTVDGMPRFYARIRKVFSPEFKLQISWFEPHPDDKGEIEWCDAELPIACGKY 600
           V+Q+WAIYD +DGMPRFYARIRKVF+PEFKL+ +W EP PDD  EI W   ELP ACGK+
Sbjct: 541 VDQIWAIYDPIDGMPRFYARIRKVFAPEFKLRFTWLEPSPDDASEIAWVKNELPYACGKF 600

Query: 601 TLGGSELTAELPMFSHMVHCPKQGASKSSYFMYPRKGETWALFKDWDIRWSSEPEKHVAF 660
           T G +E TA+LPMFSH VH  K G  ++SYF+YPRKGETWA++K+W+  WSS PE H  +
Sbjct: 601 TYGQTEETADLPMFSHQVHGEK-GGIRNSYFVYPRKGETWAIYKNWNTDWSSNPEIHRKY 660

Query: 661 EFEFVEILSDYVEGVGISVAFMDKVKDFVCLFHTTEKHRQNSFKIPPNELYRFSHQIPSV 720
           EFE+VEILSD+V   GI VA++ KVK FV LF  + +H    F+IPP+EL RFSH+IPS 
Sbjct: 661 EFEYVEILSDFVPDAGIGVAYLGKVKGFVSLFRQSVQHGIVLFQIPPSELLRFSHRIPSF 720

Query: 721 RMTGKERKGVPKGSFELDPAALPPNIND--EHVDLNNVKEETNDAPASSGKTDSSHGFKS 780
           RMTG E +GVPKGSFELDPAALP N+ND   + DL   KE  N A  +  +T      KS
Sbjct: 721 RMTGSEGEGVPKGSFELDPAALPNNLNDFSGNDDLKTEKESVN-AGVNGSRT------KS 780

Query: 781 PKEKVEVIVLDNNEAAKIQKSNLKKSHPNSEVPTTVRKSPRKLN-LTESDAQVDKFVPED 840
           P+ +++ +    N    I+    +++    E  + +R+SPR+LN L + D QV++     
Sbjct: 781 PENEMKSM----NNPTMIKPMKHEENDTERET-SELRRSPRELNGLYKKDGQVNQSECA- 840

Query: 841 NRSRDGSRNGLSTHKESSAIHQN---GGTSTPKKHGESSGLRGTTCLRIRKSPRDLSKKN 847
           N++  G +N     +   +I+ N      +TPKKH E   L  T   ++R+SPR L+KK+
Sbjct: 841 NQAEIGDKNHGDLTQSKGSIYVNLADERINTPKKH-EKDDLE-TGNFKLRRSPRALNKKH 880

BLAST of Cucsa.163790 vs. NCBI nr
Match: gi|359487946|ref|XP_002266568.2| (PREDICTED: uncharacterized protein LOC100255461 [Vitis vinifera])

HSP 1 Score: 108.6 bits (270), Expect = 5.4e-20
Identity = 70/224 (31.25%), Postives = 114/224 (50.89%), Query Frame = 1

Query: 475  DPEFSDFDTDKGKDCFAVNQVWAIYDTVDGMPRFYARIRKVF-SPEFKLQISWFEPHPDD 534
            + +F DF  +K ++ F   Q+WA+Y  VD MP+ YA+++K+  +P F+L + + E     
Sbjct: 952  EAQFYDFSGEKSEEKFQTGQLWALYSEVDRMPKNYAQVKKIEPTPSFRLHVVFLEACSPP 1011

Query: 535  KGEIEWCDAELPIACGKYTL-GGSELTAELPMFSHMVHCPKQGASKSSYFMYPRKGETWA 594
            K  ++      P+ CG + L  G         FSH +     G  K+ + + P KG+ WA
Sbjct: 1012 KDMVQ------PVCCGTFKLKNGKTKVFPRADFSHQIRAESIG--KNKFAILPIKGQVWA 1071

Query: 595  LFKDWDIRWSSEPEKHVAFEFEFVEILSDYVEGVGISVAFMDKVKDFVCLFHTTEKHRQN 654
            L+K+W+          V  +++ VE+L D      +SV     +  F  ++    + R +
Sbjct: 1072 LYKNWENNLMC--SDIVNCKYDIVEVLEDNDHSTKVSVLL--PLNGFKSVYKAPRRQRSS 1131

Query: 655  S--FKIPPNELYRFSHQIPSVRMTGKERKGVPKGSFELDPAALP 695
            +    IP +EL RFSHQIP+VR TG E        +ELDPA++P
Sbjct: 1132 TGILDIPRDELPRFSHQIPAVRHTG-ENDARLADCWELDPASVP 1162

HSP 2 Score: 669.1 bits (1725), Expect = 1.0e-188
Identity = 394/891 (44.22%), Postives = 537/891 (60.27%), Query Frame = 1

Query: 1   MECNKEEAIKAMKIAETKLEISDFIGARKMAQTAQRLFPTLDNITQLLTVCEIHCSAQNR 60
           MECNKEEA++AM++AETK++  DF GA K AQ AQRLFP LD I+ LL VCE+HCSA+ +
Sbjct: 1   MECNKEEAMRAMQLAETKMQNKDFTGAMKFAQKAQRLFPELDKISHLLAVCEVHCSAEKK 60

Query: 61  MYGAENDWYGILQIEQSADEAIIKKQYRKLALLLHPDKNKFAGAEAAFKLVGEANRLLSD 120
           M  +E DWYG+LQIEQ+ D+A I+KQYRKLALLLHPDKNKFAGAEAAFKL+GEANR+L+D
Sbjct: 61  MGVSEMDWYGVLQIEQTVDDATIRKQYRKLALLLHPDKNKFAGAEAAFKLIGEANRVLTD 120

Query: 121 QSKRKLYDLKYGAARRNIAPAKSSHDQQNGYTAVNKQERGTANGYSSGPFSHYPGGNSFK 180
            SKR +YD+KY    R      S H Q NG T+V K   GTAN   + P     G  S+ 
Sbjct: 121 HSKRSIYDMKYRGLMRTAVVNPSLH-QSNGSTSVQK-HYGTANNQHNIPHPQSTGWQSY- 180

Query: 181 PPQPPAQQAFWTCCPFCNVRYQYLKCYLSKMLRCQNCGRGFISHDLNNQTIPPTFHQMHV 240
             Q      FWTCCP+C +R+QY + +++++L C +C + F++ DL +Q +PP   Q   
Sbjct: 181 --QQTTLLTFWTCCPYCKIRFQYYRNFVNRVLSCHSCSQHFVAQDLGSQGVPPRSFQSQF 240

Query: 241 PQKKVAPESGPSKPAAENKQG--SVKKSQDRSGGVDLNAKAGKKQKGQGSNAKPKADAE- 300
             +K A   GP K ++ N +G  S  K QDR       +K G   +  G   +PK + + 
Sbjct: 241 DNQKEAQNQGPPKVSSHNSKGNHSGTKFQDRFSSFHPASKTGNAAEHSG---RPKMEGKD 300

Query: 301 ---------KTGKEKAKSD-ATSTEKVATKSQNRKRQRK---SATAHGNNSEHGDDEVEV 360
                    K G E  KS+ A S E   +K+ NRKR++K   S+ +    + +  ++V  
Sbjct: 301 KQHVDVGVGKKGAEMPKSNPAKSKEPDTSKNANRKRRKKVVESSESCETGNTNDTEDVTQ 360

Query: 361 DNVSEKDPGLSRDNCQRRSTRNKRQVSYRKYLNEDD-DSLQSPNKSSGTASTDLKEE--- 420
           +NV +       DN  RRS+R K+ VSYR+ +++DD D    P K   T S+   +E   
Sbjct: 361 ENVIDSSTPCEGDN-PRRSSRQKQNVSYRENVSDDDKDFASRPKKPRETISSCASDENMK 420

Query: 421 --------MKDATSNVEASAKGMKQEV----LPPHPEDSPNR-KPKCE-EVLREGKNGSD 480
                    KD  S   AS  G ++EV      P  E  P++ K  CE EV  E    SD
Sbjct: 421 KASVHGGVAKDDGSAAAASMDGHEKEVKHNLTVPVEESLPSKIKKTCESEVKEEEAVISD 480

Query: 481 KNDNKSKTEIVDTEENGLQGGVHVLVCADPEFSDFDTDKGKDCFAVNQVWAIYDTVDGMP 540
             D   K +     ++    G  +L   DPEF+DFD DK ++CFA+NQVWAIYDT DGMP
Sbjct: 481 HLDQNCKADDGAEVKSSHMSGPQILTYPDPEFNDFDKDKAENCFAINQVWAIYDTFDGMP 540

Query: 541 RFYARIRKVFSPEFKLQISWFEPHPDDKGEIEWCDAELPIACGKYTLGGSELTAELPMFS 600
           RFYARI+KVFSP FKL ISWFE  PDD+ EI+WCD +LP+ACGKY +GG++ T +  MFS
Sbjct: 541 RFYARIKKVFSPGFKLLISWFEADPDDQSEIDWCDQDLPVACGKYRIGGTDETEDRLMFS 600

Query: 601 HMVHCPKQGASKSSYFMYPRKGETWALFKDWDIRWSSEPEKHVAFEFEFVEILSDYVEGV 660
           H + C K G  + +Y +YPRKGETWAL+++WDI W S+P+KH+ +EFE+VE+LSD+VE  
Sbjct: 601 HRMQCVK-GRGRGTYMVYPRKGETWALYQNWDISWVSDPQKHMPYEFEYVEVLSDFVEDS 660

Query: 661 GISVAFMDKVKDFVCLFHTTEKHRQNSFKIPPNELYRFSHQIPSVRMTGKERKGVPKGSF 720
           GI+VA++ KVK FV LF  + +H   SF++PPNELYRFSH+IPS +MTG ER+ VP+GSF
Sbjct: 661 GITVAYLSKVKGFVSLFQLSNQHGIMSFQVPPNELYRFSHRIPSFKMTGDEREDVPRGSF 720

Query: 721 ELDPAALPPNINDEHVDLNNVKEETNDAPASSGK--TDSSHGFKSPKEKVEVIVLDNNEA 780
           ELDPAAL  N++    D ++VK++       +G    +S      P    E I      A
Sbjct: 721 ELDPAALSNNLS-MFGDHSDVKKDIGSTATEAGNFCPNSLESDVKPVMGSERI----PAA 780

Query: 781 AKIQKSNLKKSHPNSEVPTTVRKSPRKLNLTESD---AQVDKFVPEDNRSRDGSRNGLST 840
            K    +LK+  P S      R+SP K N ++++      ++ V  D+  +D  R  L+T
Sbjct: 781 KKHDNKDLKRESPIS------RRSPGKPNSSKTNYGKIDANEAVAADS-VKDIKRTSLTT 840

Query: 841 HKESSAIHQ-NGGTSTPKKHGESSGLRGTTCLRIRKSPRDLSKK----NAG 848
              S+   Q +   +TP+K G+ +   G+    +R+SPRDLSKK    NAG
Sbjct: 841 TVGSAPPSQVDERVNTPRKQGKKN--HGSEPFELRRSPRDLSKKLSQENAG 867

BLAST of Cucsa.163790 vs. NCBI nr
Match: gi|1009117424|ref|XP_015875310.1| (PREDICTED: uncharacterized protein LOC107412112 [Ziziphus jujuba])

HSP 1 Score: 102.1 bits (253), Expect = 5.0e-18
Identity = 71/219 (32.42%), Postives = 110/219 (50.23%), Query Frame = 1

Query: 480  DFDTDKGKDCFAVNQVWAIYDTVDGMPRFYARIRKVFS-PEFKLQISWFEPHPDDKGEIE 539
            D D  K K+ F + Q+WA+Y   DGMPR Y +++++ S P+F L ++  EP    K    
Sbjct: 939  DIDHPKSKEKFQLGQIWALYSDRDGMPRTYVQVKRIQSTPDFLLHVALLEPCSQPK---- 998

Query: 540  WCDAELPIACGKYTLGGSELTAELP--MFSHMVHCPKQGASKSSYFMYPRKGETWALFKD 599
              D   P++CG + +   E T   P   FSH +     G  K+ Y + P+ GE WAL+K+
Sbjct: 999  --DTSRPVSCGTFIVKDGE-TKVFPCSSFSHCLSAKHVG--KNRYEINPQIGEVWALYKN 1058

Query: 600  WDIRWSSEPEKHVAFEFEFVEILSDYVEGVGISVAFMDKVKDFVCLFHTTEKHRQNS--F 659
             +   +S        EF+ VE+L   V G    V  + +V  +  +F      R  +   
Sbjct: 1059 QNPISASSSTGEA--EFDIVEVLE--VSGKSTKVVVLSRVDGYKSMFKAPRIQRSKTGVI 1118

Query: 660  KIPPNELYRFSHQIPSVRMTGKERKGVPKGSFELDPAAL 694
            +IP ++  RFSH+IPS + TG E      G +ELDP ++
Sbjct: 1119 EIPRSDAARFSHKIPSYKHTG-ENDSRLLGYWELDPLSI 1143

HSP 2 Score: 633.3 bits (1632), Expect = 6.3e-178
Identity = 367/876 (41.89%), Postives = 516/876 (58.90%), Query Frame = 1

Query: 1   MECNKEEAIKAMKIAETKLEISDFIGARKMAQTAQRLFPTLDNITQLLTVCEIHCSAQNR 60
           MECNKEEA++AM+++E K++ +DF GARKMAQ AQRLFP L+N+ +LLTVCE+HCS++N+
Sbjct: 1   MECNKEEAVRAMQLSEIKIQSNDFTGARKMAQKAQRLFPELENVEKLLTVCEVHCSSENK 60

Query: 61  MYGAENDWYGILQIEQSADEAIIKKQYRKLALLLHPDKNKFAGAEAAFKLVGEANRLLSD 120
           + G+E DWYGILQI++  ++A IKKQYRKLALLLHPDKNKF+GAEAAFKL+GEANR+L+D
Sbjct: 61  IGGSEMDWYGILQIQKFDNDATIKKQYRKLALLLHPDKNKFSGAEAAFKLIGEANRVLAD 120

Query: 121 QSKRKLYDLKYGAARRNIAPAKSSHDQQNGYTAVNKQERGTANGYSSGPFSHYPGGNSFK 180
           Q+KR +YD+K  A  +  AP  S+H   NG   V K    TA+   + P S Y   N  +
Sbjct: 121 QAKRSVYDMKCRALAKAGAPKPSTH-PSNGNLFVRK-HNNTASNIQNSPQSQYTSMNQHQ 180

Query: 181 PPQPPAQQAFWTCCPFCNVRYQYLKCYLSKMLRCQNCGRGFISHDLNNQ-TIPPTFHQMH 240
             QP   + FWTCCPFC ++YQY + + +++LRCQ C R F++HDL  Q  + P   +  
Sbjct: 181 QAQP---ETFWTCCPFCKIKYQYHQDFANRLLRCQKCRRAFVAHDLGIQFQVHPESVRNQ 240

Query: 241 VPQKKVAPESGPSKPAAENKQGSVKKSQDR----------------SGGVDLNAKAGKKQ 300
            P +K  P  G S  A+++  G+   S  +                S  V +++ + KK 
Sbjct: 241 FPNRKEPPSQGASNVASQSNGGTGNPSSTKYQNGNAASNPLSKTGFSDDVSMDSNSEKKD 300

Query: 301 KGQGSNAKPKADAEKTGKEKAKSDATSTEKVATKSQNRKRQRKSATAHGN----NSEHGD 360
            G G          K+G  K+K    S  K   + +N   +   +   GN    +SEH  
Sbjct: 301 IGHG------VGMSKSGPVKSKDSEISRNKNKKRGRNSIFESSESCKTGNTATSDSEHVI 360

Query: 361 DEVEVDNVSEKDPGLSRDNCQRRSTRNKRQVSYRKYLNEDDDSLQSPNK------SSGTA 420
            + +V  +SE + G    N  RRS+R K+ +SY + LN DDD +  P +      SSG +
Sbjct: 361 IQEKVSKLSEPNGG----NHNRRSSRKKQNLSYNENLN-DDDFVSPPKRWRDSQLSSGVS 420

Query: 421 STDLKEEMKDATSNVEASAKGMKQEVLPPHPEDSPNRKPKCEEVLREGKNGS--DKNDNK 480
            TD   +    T++V       +Q V  P  E SP+++ K  E  +  K  +  D +D K
Sbjct: 421 KTDGSTD-SATTASVGGHKNEAEQNVTAPLGECSPSKRSKPGEFEKNVKEAAMPDNDDGK 480

Query: 481 SKTEIVDTEENGLQGGVHVLVCADPEFSDF--DTDKGKDCFAVNQVWAIYDTVDGMPRFY 540
              ++     + +      +   DPEF+ F    D  ++ F+ NQ WA+YD VDGMPRFY
Sbjct: 481 LNADVGPGPSSNVASMPASVEVPDPEFNKFGLGEDMLENVFSANQTWALYDPVDGMPRFY 540

Query: 541 ARIRKVFSPEFKLQISWFEPHPDDKGEIEWCDAELPIACGKYTLGGSELTAELPMFSHMV 600
           AR++KVF+P FK++ +W E +PDD+GEI WC+ ELP+ACGKYTLG ++   +  MFSH +
Sbjct: 541 ARVKKVFTPGFKVRFTWLESNPDDQGEIAWCNKELPVACGKYTLGHTQEVTDHLMFSHQM 600

Query: 601 HCPKQGASKSSYFMYPRKGETWALFKDWDIRWSSEPEKHVAFEFEFVEILSDYVEGVGIS 660
           HC K G+ +SS+F+YPRKGETWAL+++WDI WSSEPEKHV ++FEFVE++SD+ E  G+ 
Sbjct: 601 HCIK-GSGRSSFFVYPRKGETWALYQNWDIGWSSEPEKHVPYKFEFVEVVSDFDENNGVG 660

Query: 661 VAFMDKVKDFVCLFHTTEKHRQNSFKIPPNELYRFSHQIPSVRMTGKERKGVPKGSFELD 720
           VA++ KVK FV LF  +E+H    F++P NELYRFSH+IPS +MTG ER GVPK SFE D
Sbjct: 661 VAYLGKVKGFVSLFQRSEQHGVILFQVPRNELYRFSHRIPSFKMTGDERDGVPKESFEFD 720

Query: 721 PAALPPNINDEHVDLNNVKEETNDAPASSGKTDSSHGFKSPKEKVEVIVLDNNEAAKIQK 780
           PA+LP N++D + DL       N  P        S  F   + K    V+ +      +K
Sbjct: 721 PASLPTNLDDFN-DLKKGNRAMNTEP-----NGLSREFLESEGKP---VMGSGRVCAAEK 780

Query: 781 SNLKKSHPNSEVPTTVRKSPRKLNLTESDAQVDKFVPEDNRSRDGSRNGLSTHKESSAIH 840
                   NS   T++R+SPRK N   + +   +    D     GS         +SA  
Sbjct: 781 QE------NSVRETSMRRSPRKANSQSASSVKLEATRCDLTQPTGS---------ASACQ 832

Query: 841 QNGGTSTPKKHGESSGLRGTTCLRIRKSPRDLSKKN 846
            +    TPKKH ++   R T   R+R+SPRDLSK +
Sbjct: 841 ADEIIKTPKKHLKNDSDRET--FRLRRSPRDLSKNS 832