BLAST of Cucsa.162960 vs. Swiss-Prot
Match:
PP255_ARATH (Putative pentatricopeptide repeat-containing protein At3g25970 OS=Arabidopsis thaliana GN=PCMP-E46 PE=3 SV=2)
HSP 1 Score: 721.5 bits (1861), Expect = 8.0e-207
Identity = 347/614 (56.51%), Postives = 458/614 (74.59%), Query Frame = 1
Query: 1 MIAGHINCGNLEASWDVLRCMRSCGFELDRYTFGSMLKGIAFAGMFHLGQQVHSIIIKMG 60
MI+G+ +CG LE +W + CM+ G ++D Y+F +LKGIA F LG+QVH ++IK G
Sbjct: 72 MISGYTSCGKLEDAWCLFTCMKRSGSDVDGYSFSRLLKGIASVKRFDLGEQVHGLVIKGG 131
Query: 61 YAENVYAGSALLDMYAKCEKLEDAYLSFLSISKHNTVSWNAMINGYAQAGDRETAFWLLD 120
Y NVY GS+L+DMYAKCE++EDA+ +F IS+ N+VSWNA+I G+ Q D +TAFWLL
Sbjct: 132 YECNVYVGSSLVDMYAKCERVEDAFEAFKEISEPNSVSWNALIAGFVQVRDIKTAFWLLG 191
Query: 121 CMEQEGE-KVDDGTYAPLLPLLDDADFCNLTSQLHGKIIKHGLELVNTMCNALITSYSKC 180
ME + +D GT+APLL LLDD FCNL Q+H K++K GL+ T+CNA+I+SY+ C
Sbjct: 192 LMEMKAAVTMDAGTFAPLLTLLDDPMFCNLLKQVHAKVLKLGLQHEITICNAMISSYADC 251
Query: 181 GSLDDAKRIFDSSAGIRDLVTWNSLLAAYLLRSQEDLAFKLLIDMQEHGFEPDLYSYTSI 240
GS+ DAKR+FD G +DL++WNS++A + ++ AF+L I MQ H E D+Y+YT +
Sbjct: 252 GSVSDAKRVFDGLGGSKDLISWNSMIAGFSKHELKESAFELFIQMQRHWVETDIYTYTGL 311
Query: 241 ISACFNENISNNGRSLHGLVIKRGFEQSVPISNALISMYLKSDYGSMKEALCIFESLEFK 300
+SAC E G+SLHG+VIK+G EQ +NALISMY++ G+M++AL +FESL+ K
Sbjct: 312 LSACSGEEHQIFGKSLHGMVIKKGLEQVTSATNALISMYIQFPTGTMEDALSLFESLKSK 371
Query: 301 DRVSWNSILTGLSQTGSSEDAVKSFLHMRSAAMDIDHYSFSAVLRSCSDLATFQLGQQIH 360
D +SWNSI+TG +Q G SEDAVK F ++RS+ + +D Y+FSA+LRSCSDLAT QLGQQIH
Sbjct: 372 DLISWNSIITGFAQKGLSEDAVKFFSYLRSSEIKVDDYAFSALLRSCSDLATLQLGQQIH 431
Query: 361 VLALKYGLESNEFVSSSLIFMYSKCGIIEDARRSFEE-ASKNSSITWNALMFGYAQHGQC 420
LA K G SNEFV SSLI MYSKCGIIE AR+ F++ +SK+S++ WNA++ GYAQHG
Sbjct: 432 ALATKSGFVSNEFVISSLIVMYSKCGIIESARKCFQQISSKHSTVAWNAMILGYAQHGLG 491
Query: 421 NVALDLFFLMEEKKVKMDHITFVAVLTACSHIGLVEQGCKFLRCMESDYGVPPRMEHYAC 480
V+LDLF M + VK+DH+TF A+LTACSH GL+++G + L ME Y + PRMEHYA
Sbjct: 492 QVSLDLFSQMCNQNVKLDHVTFTAILTACSHTGLIQEGLELLNLMEPVYKIQPRMEHYAA 551
Query: 481 AVDLYGRSGRLEEAKALIEEMPFKPDTTVWKTFLGACRSCGNIELACQVAGHLLEMEPEE 540
AVDL GR+G + +AK LIE MP PD V KTFLG CR+CG IE+A QVA HLLE+EPE+
Sbjct: 552 AVDLLGRAGLVNKAKELIESMPLNPDPMVLKTFLGVCRACGEIEMATQVANHLLEIEPED 611
Query: 541 HCTYVLLSNMYGNLMRWDEKAKVKRLMKERGVKKVPGWSWIEVNNNVHAFIAQDHSHPSC 600
H TYV LS+MY +L +W+EKA VK++MKERGVKKVPGWSWIE+ N V AF A+D S+P C
Sbjct: 612 HFTYVSLSHMYSDLKKWEEKASVKKMMKERGVKKVPGWSWIEIRNQVKAFNAEDRSNPLC 671
Query: 601 QQIYFLLEVLLEEI 613
Q IY +++ L +E+
Sbjct: 672 QDIYMMIKDLTQEM 685
HSP 2 Score: 213.0 bits (541), Expect = 9.3e-54
Identity = 133/464 (28.66%), Postives = 236/464 (50.86%), Query Frame = 1
Query: 53 HSIIIKMGYAENVYAGSALLDMYAKCEKLEDAYLSFLSISKHNTVSWNAMINGYAQAGDR 112
H IK G ++Y + +LD Y K L A + F + K ++VSWN MI+GY G
Sbjct: 23 HCYAIKCGSISDIYVSNRILDSYIKFGFLGYANMLFDEMPKRDSVSWNTMISGYTSCGKL 82
Query: 113 ETAFWLLDCMEQEGEKVDDGTYAPLLPLLDDADFCNLTSQLHGKIIKHGLELVNTMCNAL 172
E A+ L CM++ G VD +++ LL + +L Q+HG +IK G E + ++L
Sbjct: 83 EDAWCLFTCMKRSGSDVDGYSFSRLLKGIASVKRFDLGEQVHGLVIKGGYECNVYVGSSL 142
Query: 173 ITSYSKCGSLDDAKRIFDSSAGIRDLVTWNSLLAAYLLRSQEDLAFKLLIDMQ-EHGFEP 232
+ Y+KC ++DA F + V+WN+L+A ++ AF LL M+ +
Sbjct: 143 VDMYAKCERVEDAFEAF-KEISEPNSVSWNALIAGFVQVRDIKTAFWLLGLMEMKAAVTM 202
Query: 233 DLYSYTSIISACFNENISNNGRSLHGLVIKRGFEQSVPISNALISMYLKSDYGSMKEALC 292
D ++ +++ + N + +H V+K G + + I NA+IS Y +D GS+ +A
Sbjct: 203 DAGTFAPLLTLLDDPMFCNLLKQVHAKVLKLGLQHEITICNAMISSY--ADCGSVSDAKR 262
Query: 293 IFESL-EFKDRVSWNSILTGLSQTGSSEDAVKSFLHMRSAAMDIDHYSFSAVLRSCSDLA 352
+F+ L KD +SWNS++ G S+ E A + F+ M+ ++ D Y+++ +L +CS
Sbjct: 263 VFDGLGGSKDLISWNSMIAGFSKHELKESAFELFIQMQRHWVETDIYTYTGLLSACSGEE 322
Query: 353 TFQLGQQIHVLALKYGLESNEFVSSSLIFMYSK--CGIIEDARRSFEEASKNSSITWNAL 412
G+ +H + +K GLE +++LI MY + G +EDA FE I+WN++
Sbjct: 323 HQIFGKSLHGMVIKKGLEQVTSATNALISMYIQFPTGTMEDALSLFESLKSKDLISWNSI 382
Query: 413 MFGYAQHGQCNVALDLFFLMEEKKVKMDHITFVAVLTACSHIGLVEQGCKFLRCMESDYG 472
+ G+AQ G A+ F + ++K+D F A+L +CS + ++ G + + + + G
Sbjct: 383 ITGFAQKGLSEDAVKFFSYLRSSEIKVDDYAFSALLRSCSDLATLQLG-QQIHALATKSG 442
Query: 473 VPPRMEHYACAVDLYGRSGRLEEAKALIEEMPFKPDTTVWKTFL 513
+ + +Y + G +E A+ +++ K T W +
Sbjct: 443 FVSNEFVISSLIVMYSKCGIIESARKCFQQISSKHSTVAWNAMI 482
HSP 3 Score: 62.8 bits (151), Expect = 1.6e-08
Identity = 48/189 (25.40%), Postives = 89/189 (47.09%), Query Frame = 1
Query: 347 SDLATFQLGQQIHVLALKYGLESNEFVSSSLIFMYSKCGIIEDARRSFEEASKNSSITWN 406
S L +FQ H A+K G S+ +VS+ ++ Y K G + A F+E K S++WN
Sbjct: 11 SSLNSFQKLSLTHCYAIKCGSISDIYVSNRILDSYIKFGFLGYANMLFDEMPKRDSVSWN 70
Query: 407 ALMFGYAQHGQCNVALDLFFLMEEKKVKMDHITFVAVLTACSHIGLVEQGCKFLRCMESD 466
++ GY G+ A LF M+ +D +F +L + + + G + + +
Sbjct: 71 TMISGYTSCGKLEDAWCLFTCMKRSGSDVDGYSFSRLLKGIASVKRFDLG-EQVHGLVIK 130
Query: 467 YGVPPRMEHYACAVDLYGRSGRLEEAKALIEEMPFKPDTTVWKTFLGACRSCGNIELACQ 526
G + + VD+Y + R+E+A +E+ +P++ W + +I+ A
Sbjct: 131 GGYECNVYVGSSLVDMYAKCERVEDAFEAFKEIS-EPNSVSWNALIAGFVQVRDIKTAFW 190
Query: 527 VAGHLLEME 536
+ G L+EM+
Sbjct: 191 LLG-LMEMK 196
BLAST of Cucsa.162960 vs. Swiss-Prot
Match:
PP307_ARATH (Pentatricopeptide repeat-containing protein At4g13650 OS=Arabidopsis thaliana GN=PCMP-H42 PE=2 SV=2)
HSP 1 Score: 428.7 bits (1101), Expect = 1.1e-118
Identity = 222/602 (36.88%), Postives = 339/602 (56.31%), Query Frame = 1
Query: 1 MIAGHINCGNLEASWDVLRCMRSCGFELDRYTFGSMLKGIAFAGMFHLGQQVHSIIIKMG 60
+I G CG E + ++ + M G E D T S++ + G GQQ+H+ K+G
Sbjct: 360 LINGLSQCGYGEKAMELFKRMHLDGLEPDSNTLASLVVACSADGTLFRGQQLHAYTTKLG 419
Query: 61 YAENVYAGSALLDMYAKCEKLEDAYLSFLSISKHNTVSWNAMINGYAQAGDRETAFWLLD 120
+A N ALL++YAKC +E A FL N V WN M+ Y D +F +
Sbjct: 420 FASNNKIEGALLNLYAKCADIETALDYFLETEVENVVLWNVMLVAYGLLDDLRNSFRIFR 479
Query: 121 CMEQEGEKVDDGTYAPLLPLLDDADFCNLTSQLHGKIIKHGLELVNTMCNALITSYSKCG 180
M+ E + TY +L L Q+H +IIK +L +C+ LI Y+K G
Sbjct: 480 QMQIEEIVPNQYTYPSILKTCIRLGDLELGEQIHSQIIKTNFQLNAYVCSVLIDMYAKLG 539
Query: 181 SLDDAKRIFDSSAGIRDLVTWNSLLAAYLLRSQEDLAFKLLIDMQEHGFEPDLYSYTSII 240
LD A I AG +D+V+W +++A Y + +D A M + G D T+ +
Sbjct: 540 KLDTAWDILIRFAG-KDVVSWTTMIAGYTQYNFDDKALTTFRQMLDRGIRSDEVGLTNAV 599
Query: 241 SACFNENISNNGRSLHGLVIKRGFEQSVPISNALISMYLKSDYGSMKEALCIFESLEFKD 300
SAC G+ +H GF +P NAL+++Y S G ++E+ FE E D
Sbjct: 600 SACAGLQALKEGQQIHAQACVSGFSSDLPFQNALVTLY--SRCGKIEESYLAFEQTEAGD 659
Query: 301 RVSWNSILTGLSQTGSSEDAVKSFLHMRSAAMDIDHYSFSAVLRSCSDLATFQLGQQIHV 360
++WN++++G Q+G++E+A++ F+ M +D ++++F + +++ S+ A + G+Q+H
Sbjct: 660 NIAWNALVSGFQQSGNNEEALRVFVRMNREGIDNNNFTFGSAVKAASETANMKQGKQVHA 719
Query: 361 LALKYGLESNEFVSSSLIFMYSKCGIIEDARRSFEEASKNSSITWNALMFGYAQHGQCNV 420
+ K G +S V ++LI MY+KCG I DA + F E S + ++WNA++ Y++HG +
Sbjct: 720 VITKTGYDSETEVCNALISMYAKCGSISDAEKQFLEVSTKNEVSWNAIINAYSKHGFGSE 779
Query: 421 ALDLFFLMEEKKVKMDHITFVAVLTACSHIGLVEQGCKFLRCMESDYGVPPRMEHYACAV 480
ALD F M V+ +H+T V VL+ACSHIGLV++G + M S+YG+ P+ EHY C V
Sbjct: 780 ALDSFDQMIHSNVRPNHVTLVGVLSACSHIGLVDKGIAYFESMNSEYGLSPKPEHYVCVV 839
Query: 481 DLYGRSGRLEEAKALIEEMPFKPDTTVWKTFLGACRSCGNIELACQVAGHLLEMEPEEHC 540
D+ R+G L AK I+EMP KPD VW+T L AC N+E+ A HLLE+EPE+
Sbjct: 840 DMLTRAGLLSRAKEFIQEMPIKPDALVWRTLLSACVVHKNMEIGEFAAHHLLELEPEDSA 899
Query: 541 TYVLLSNMYGNLMRWDEKAKVKRLMKERGVKKVPGWSWIEVNNNVHAFIAQDHSHPSCQQ 600
TYVLLSN+Y +WD + ++ MKE+GVKK PG SWIEV N++H+F D +HP +
Sbjct: 900 TYVLLSNLYAVSKKWDARDLTRQKMKEKGVKKEPGQSWIEVKNSIHSFYVGDQNHPLADE 958
Query: 601 IY 603
I+
Sbjct: 960 IH 958
HSP 2 Score: 211.8 bits (538), Expect = 2.1e-53
Identity = 133/484 (27.48%), Postives = 229/484 (47.31%), Query Frame = 1
Query: 31 YTFGSMLKGIAFAGMFHLGQQVHSIIIKMGYAENVYAGSALLDMYAKCEKLEDAYLSFLS 90
Y F S+L +G+Q+H +++K+G++ + Y +AL+ +Y L A F +
Sbjct: 289 YAFSSVLSACKKIESLEIGEQLHGLVLKLGFSSDTYVCNALVSLYFHLGNLISAEHIFSN 348
Query: 91 ISKHNTVSWNAMINGYAQAGDRETAFWLLDCMEQEGEKVDDGTYAPLLPLLDDADFCNLT 150
+S+ + V++N +ING +Q G E A L M +G + D T A L+
Sbjct: 349 MSQRDAVTYNTLINGLSQCGYGEKAMELFKRMHLDGLEPDSNTLASLVVACSADGTLFRG 408
Query: 151 SQLHGKIIKHGLELVNTMCNALITSYSKCGSLDDAKRIFDSSAGIRDLVTWNSLLAAYLL 210
QLH K G N + AL+ Y+KC ++ A F + + ++V WN +L AY L
Sbjct: 409 QQLHAYTTKLGFASNNKIEGALLNLYAKCADIETALDYFLETE-VENVVLWNVMLVAYGL 468
Query: 211 RSQEDLAFKLLIDMQEHGFEPDLYSYTSIISACFNENISNNGRSLHGLVIKRGFEQSVPI 270
+F++ MQ P+ Y+Y SI+ C G +H +IK F+ + +
Sbjct: 469 LDDLRNSFRIFRQMQIEEIVPNQYTYPSILKTCIRLGDLELGEQIHSQIIKTNFQLNAYV 528
Query: 271 SNALISMYLKSDYGSMKEALCIFESLEFKDRVSWNSILTGLSQTGSSEDAVKSFLHMRSA 330
+ LI MY K G + A I KD VSW +++ G +Q + A+ +F M
Sbjct: 529 CSVLIDMYAK--LGKLDTAWDILIRFAGKDVVSWTTMIAGYTQYNFDDKALTTFRQMLDR 588
Query: 331 AMDIDHYSFSAVLRSCSDLATFQLGQQIHVLALKYGLESNEFVSSSLIFMYSKCGIIEDA 390
+ D + + +C+ L + GQQIH A G S+ ++L+ +YS+CG IE++
Sbjct: 589 GIRSDEVGLTNAVSACAGLQALKEGQQIHAQACVSGFSSDLPFQNALVTLYSRCGKIEES 648
Query: 391 RRSFEEASKNSSITWNALMFGYAQHGQCNVALDLFFLMEEKKVKMDHITFVAVLTACSHI 450
+FE+ +I WNAL+ G+ Q G AL +F M + + ++ TF + + A S
Sbjct: 649 YLAFEQTEAGDNIAWNALVSGFQQSGNNEEALRVFVRMNREGIDNNNFTFGSAVKAASET 708
Query: 451 GLVEQGCKFLRCMESDYGVPPRMEHYACAVDLYGRSGRLEEAKALIEEMPFKPDTTVWKT 510
++QG K + + + G E + +Y + G + +A+ E+ K + + W
Sbjct: 709 ANMKQG-KQVHAVITKTGYDSETEVCNALISMYAKCGSISDAEKQFLEVSTKNEVS-WNA 767
Query: 511 FLGA 515
+ A
Sbjct: 769 IINA 767
HSP 3 Score: 203.0 bits (515), Expect = 9.6e-51
Identity = 135/493 (27.38%), Postives = 239/493 (48.48%), Query Frame = 1
Query: 25 GFELDRYTFGSMLKG-IAFAGMFHLGQQVHSIIIKMGYAENVYAGSALLDMYAKCEKLED 84
G + T +L+G + G G+++HS I+K+G N L D Y L
Sbjct: 79 GIRPNHQTLKWLLEGCLKTNGSLDEGRKLHSQILKLGLDSNGCLSEKLFDFYLFKGDLYG 138
Query: 85 AYLSFLSISKHNTVSWNAMINGYAQAGDRETAFWLLDCMEQEGEKVDDGTYAPLLPLLDD 144
A+ F + + +WN MI A F L M E ++GT++ +L
Sbjct: 139 AFKVFDEMPERTIFTWNKMIKELASRNLIGEVFGLFVRMVSENVTPNEGTFSGVLEACRG 198
Query: 145 ADFC-NLTSQLHGKIIKHGLELVNTMCNALITSYSKCGSLDDAKRIFDSSAGIRDLVTWN 204
++ Q+H +I+ GL +CN LI YS+ G +D A+R+FD ++D +W
Sbjct: 199 GSVAFDVVEQIHARILYQGLRDSTVVCNPLIDLYSRNGFVDLARRVFDGLR-LKDHSSWV 258
Query: 205 SLLAAYLLRSQEDLAFKLLIDMQEHGFEPDLYSYTSIISACFNENISNNGRSLHGLVIKR 264
++++ E A +L DM G P Y+++S++SAC G LHGLV+K
Sbjct: 259 AMISGLSKNECEAEAIRLFCDMYVLGIMPTPYAFSSVLSACKKIESLEIGEQLHGLVLKL 318
Query: 265 GFEQSVPISNALISMYLKSDYGSMKEALCIFESLEFKDRVSWNSILTGLSQTGSSEDAVK 324
GF + NAL+S+Y G++ A IF ++ +D V++N+++ GLSQ G E A++
Sbjct: 319 GFSSDTYVCNALVSLYFH--LGNLISAEHIFSNMSQRDAVTYNTLINGLSQCGYGEKAME 378
Query: 325 SFLHMRSAAMDIDHYSFSAVLRSCSDLATFQLGQQIHVLALKYGLESNEFVSSSLIFMYS 384
F M ++ D + ++++ +CS T GQQ+H K G SN + +L+ +Y+
Sbjct: 379 LFKRMHLDGLEPDSNTLASLVVACSADGTLFRGQQLHAYTTKLGFASNNKIEGALLNLYA 438
Query: 385 KCGIIEDARRSFEEASKNSSITWNALMFGYAQHGQCNVALDLFFLMEEKKVKMDHITFVA 444
KC IE A F E + + WN ++ Y + +F M+ +++ + T+ +
Sbjct: 439 KCADIETALDYFLETEVENVVLWNVMLVAYGLLDDLRNSFRIFRQMQIEEIVPNQYTYPS 498
Query: 445 VLTACSHIGLVEQGCKF-LRCMESDYGVPPRMEHYACAV--DLYGRSGRLEEAKALIEEM 504
+L C +G +E G + + +++++ ++ Y C+V D+Y + G+L+ A ++
Sbjct: 499 ILKTCIRLGDLELGEQIHSQIIKTNF----QLNAYVCSVLIDMYAKLGKLDTAWDILIRF 558
Query: 505 PFKPDTTVWKTFL 513
K D W T +
Sbjct: 559 AGK-DVVSWTTMI 563
HSP 4 Score: 177.6 bits (449), Expect = 4.3e-43
Identity = 126/497 (25.35%), Postives = 229/497 (46.08%), Query Frame = 1
Query: 32 TFGSMLKGIAFAGM-FHLGQQVHSIIIKMGYAENVYAGSALLDMYAKCEKLEDAYLSFLS 91
TF +L+ + F + +Q+H+ I+ G ++ + L+D+Y++ ++ A F
Sbjct: 188 TFSGVLEACRGGSVAFDVVEQIHARILYQGLRDSTVVCNPLIDLYSRNGFVDLARRVFDG 247
Query: 92 ISKHNTVSWNAMINGYAQAGDRETAFWLLDCMEQEGEKVDDGTYAPLLPLLDDADFCNLT 151
+ + SW AMI+G ++ A L M G ++ +L + +
Sbjct: 248 LRLKDHSSWVAMISGLSKNECEAEAIRLFCDMYVLGIMPTPYAFSSVLSACKKIESLEIG 307
Query: 152 SQLHGKIIKHGLELVNTMCNALITSYSKCGSLDDAKRIFDSSAGIRDLVTWNSLLAAYLL 211
QLHG ++K G +CNAL++ Y G+L A+ IF S+ RD VT+N+L+
Sbjct: 308 EQLHGLVLKLGFSSDTYVCNALVSLYFHLGNLISAEHIF-SNMSQRDAVTYNTLINGLSQ 367
Query: 212 RSQEDLAFKLLIDMQEHGFEPDLYSYTSIISACFNENISNNGRSLHGLVIKRGFEQSVPI 271
+ A +L M G EPD + S++ AC + G+ LH K GF + I
Sbjct: 368 CGYGEKAMELFKRMHLDGLEPDSNTLASLVVACSADGTLFRGQQLHAYTTKLGFASNNKI 427
Query: 272 SNALISMYLKSDYGSMKEALCIFESLEFKDRVSWNSILTGLSQTGSSEDAVKSFLHMRSA 331
AL+++Y K ++ AL F E ++ V WN +L ++ + F M+
Sbjct: 428 EGALLNLYAKC--ADIETALDYFLETEVENVVLWNVMLVAYGLLDDLRNSFRIFRQMQIE 487
Query: 332 AMDIDHYSFSAVLRSCSDLATFQLGQQIHVLALKYGLESNEFVSSSLIFMYSKCGIIEDA 391
+ + Y++ ++L++C L +LG+QIH +K + N +V S LI MY+K G ++ A
Sbjct: 488 EIVPNQYTYPSILKTCIRLGDLELGEQIHSQIIKTNFQLNAYVCSVLIDMYAKLGKLDTA 547
Query: 392 RRSFEEASKNSSITWNALMFGYAQHGQCNVALDLFFLMEEKKVKMDHITFVAVLTACSHI 451
+ ++W ++ GY Q+ + AL F M ++ ++ D + ++AC+ +
Sbjct: 548 WDILIRFAGKDVVSWTTMIAGYTQYNFDDKALTTFRQMLDRGIRSDEVGLTNAVSACAGL 607
Query: 452 GLVEQGCKFLRCMESDYGVPPRMEHYACAVDLYGRSGRLEEAKALIEEMPFKPDTTVWKT 511
+++G + + G + V LY R G++EE+ E+ D W
Sbjct: 608 QALKEG-QQIHAQACVSGFSSDLPFQNALVTLYSRCGKIEESYLAFEQTE-AGDNIAWNA 667
Query: 512 FLGACRSCGNIELACQV 528
+ + GN E A +V
Sbjct: 668 LVSGFQQSGNNEEALRV 679
HSP 5 Score: 99.8 bits (247), Expect = 1.1e-19
Identity = 96/371 (25.88%), Postives = 165/371 (44.47%), Query Frame = 1
Query: 200 TWNSLLAAYLLRSQEDLAF--KLLIDMQEHGFEPDLYSYTSIISACFNENIS-NNGRSLH 259
T + AA + ED +F K + ++ G P+ + ++ C N S + GR LH
Sbjct: 49 TRRASFAAISVYISEDESFQEKRIDSVENRGIRPNHQTLKWLLEGCLKTNGSLDEGRKLH 108
Query: 260 GLVIKRGFEQSVPISNALISMYL-KSD-YGSMKEALCIFESLEFKDRVSWNSILTGLSQT 319
++K G + + +S L YL K D YG+ K +F+ + + +WN ++ L+
Sbjct: 109 SQILKLGLDSNGCLSEKLFDFYLFKGDLYGAFK----VFDEMPERTIFTWNKMIKELASR 168
Query: 320 GSSEDAVKSFLHMRSAAMDIDHYSFSAVLRSC-SDLATFQLGQQIHVLALKYGLESNEFV 379
+ F+ M S + + +FS VL +C F + +QIH L GL + V
Sbjct: 169 NLIGEVFGLFVRMVSENVTPNEGTFSGVLEACRGGSVAFDVVEQIHARILYQGLRDSTVV 228
Query: 380 SSSLIFMYSKCGIIEDARRSFEEASKNSSITWNALMFGYAQHGQCNVALDLFFLMEEKKV 439
+ LI +YS+ G ++ ARR F+ +W A++ G +++ A+ LF M +
Sbjct: 229 CNPLIDLYSRNGFVDLARRVFDGLRLKDHSSWVAMISGLSKNECEAEAIRLFCDMYVLGI 288
Query: 440 KMDHITFVAVLTACSHIGLVEQGCKFLRCMESDYGVPPRM----EHYAC--AVDLYGRSG 499
F +VL+AC I +E G E +G+ ++ + Y C V LY G
Sbjct: 289 MPTPYAFSSVLSACKKIESLEIG-------EQLHGLVLKLGFSSDTYVCNALVSLYFHLG 348
Query: 500 RLEEAKALIEEMPFKPDTTVWKTFLGACRSCGNIELACQV--AGHLLEMEPEEH--CTYV 555
L A+ + M + D + T + CG E A ++ HL +EP+ + + V
Sbjct: 349 NLISAEHIFSNMS-QRDAVTYNTLINGLSQCGYGEKAMELFKRMHLDGLEPDSNTLASLV 407
BLAST of Cucsa.162960 vs. Swiss-Prot
Match:
PP172_ARATH (Pentatricopeptide repeat-containing protein At2g27610 OS=Arabidopsis thaliana GN=PCMP-H60 PE=2 SV=1)
HSP 1 Score: 404.1 bits (1037), Expect = 2.8e-111
Identity = 216/598 (36.12%), Postives = 352/598 (58.86%), Query Frame = 1
Query: 21 MRSCGFELDRYTFGSMLKGIAFAGMFHLGQQVHSIIIKMGYAENVYAGSALLDMYAKCEK 80
M++ G + + +TF + L +A G+ G QVH++++K G + + ++L+++Y KC
Sbjct: 185 MQNEGTQPNSFTFAAALGVLAEEGVGGRGLQVHTVVVKNGLDKTIPVSNSLINLYLKCGN 244
Query: 81 LEDAYLSFLSISKHNTVSWNAMINGYAQAGDRETAFWLLDCMEQEGEKVDDGTYAPLLPL 140
+ A + F + V+WN+MI+GYA G A + M ++ + ++A ++ L
Sbjct: 245 VRKARILFDKTEVKSVVTWNSMISGYAANGLDLEALGMFYSMRLNYVRLSESSFASVIKL 304
Query: 141 LDDADFCNLTSQLHGKIIKHGLELVNTMCNALITSYSKCGSLDDAKRIFDSSAGIRDLVT 200
+ T QLH ++K+G + AL+ +YSKC ++ DA R+F + ++V+
Sbjct: 305 CANLKELRFTEQLHCSVVKYGFLFDQNIRTALMVAYSKCTAMLDALRLFKEIGCVGNVVS 364
Query: 201 WNSLLAAYLLRSQEDLAFKLLIDMQEHGFEPDLYSYTSIISACFNENISNNGRSLHGLVI 260
W ++++ +L ++ A L +M+ G P+ ++Y+ I++A + S +H V+
Sbjct: 365 WTAMISGFLQNDGKEEAVDLFSEMKRKGVRPNEFTYSVILTALPVISPSE----VHAQVV 424
Query: 261 KRGFEQSVPISNALISMYLKSDYGSMKEALCIFESLEFKDRVSWNSILTGLSQTGSSEDA 320
K +E+S + AL+ Y+K G ++EA +F ++ KD V+W+++L G +QTG +E A
Sbjct: 425 KTNYERSSTVGTALLDAYVK--LGKVEEAAKVFSGIDDKDIVAWSAMLAGYAQTGETEAA 484
Query: 321 VKSFLHMRSAAMDIDHYSFSAVLRSCSDL-ATFQLGQQIHVLALKYGLESNEFVSSSLIF 380
+K F + + + ++FS++L C+ A+ G+Q H A+K L+S+ VSS+L+
Sbjct: 485 IKMFGELTKGGIKPNEFTFSSILNVCAATNASMGQGKQFHGFAIKSRLDSSLCVSSALLT 544
Query: 381 MYSKCGIIEDARRSFEEASKNSSITWNALMFGYAQHGQCNVALDLFFLMEEKKVKMDHIT 440
MY+K G IE A F+ + ++WN+++ GYAQHGQ ALD+F M+++KVKMD +T
Sbjct: 545 MYAKKGNIESAEEVFKRQREKDLVSWNSMISGYAQHGQAMKALDVFKEMKKRKVKMDGVT 604
Query: 441 FVAVLTACSHIGLVEQGCKFLRCMESDYGVPPRMEHYACAVDLYGRSGRLEEAKALIEEM 500
F+ V AC+H GLVE+G K+ M D + P EH +C VDLY R+G+LE+A +IE M
Sbjct: 605 FIGVFAACTHAGLVEEGEKYFDIMVRDCKIAPTKEHNSCMVDLYSRAGQLEKAMKVIENM 664
Query: 501 PFKPDTTVWKTFLGACRSCGNIELACQVAGHLLEMEPEEHCTYVLLSNMYGNLMRWDEKA 560
P +T+W+T L ACR EL A ++ M+PE+ YVLLSNMY W E+A
Sbjct: 665 PNPAGSTIWRTILAACRVHKKTELGRLAAEKIIAMKPEDSAAYVLLSNMYAESGDWQERA 724
Query: 561 KVKRLMKERGVKKVPGWSWIEVNNNVHAFIAQDHSHPSCQQIYFLLEVLLEEITRMED 618
KV++LM ER VKK PG+SWIEV N ++F+A D SHP QIY LE L TR++D
Sbjct: 725 KVRKLMNERNVKKEPGYSWIEVKNKTYSFLAGDRSHPLKDQIYMKLEDL---STRLKD 773
HSP 2 Score: 196.4 bits (498), Expect = 9.0e-49
Identity = 132/509 (25.93%), Postives = 242/509 (47.54%), Query Frame = 1
Query: 25 GFELDRYTFGSMLKGIAFAGMFHLGQQVHSIIIKMGYAENVYAGSALLDMYAKCEKLEDA 84
G E+D F S+LK A G+Q+H IK G+ ++V G++L+D Y K +D
Sbjct: 88 GMEMDCSIFSSVLKVSATLCDELFGRQLHCQCIKFGFLDDVSVGTSLVDTYMKGSNFKDG 147
Query: 85 YLSFLSISKHNTVSWNAMINGYAQAGDRETAFWLLDCMEQEGEKVDDGTYAPLLPLLDDA 144
F + + N V+W +I+GYA+ + L M+ EG + + T+A L +L +
Sbjct: 148 RKVFDEMKERNVVTWTTLISGYARNSMNDEVLTLFMRMQNEGTQPNSFTFAAALGVLAEE 207
Query: 145 DFCNLTSQLHGKIIKHGLELVNTMCNALITSYSKCGSLDDAKRIFDSSAGIRDLVTWNSL 204
Q+H ++K+GL+ + N+LI Y KCG++ A+ +FD + ++ +VTWNS+
Sbjct: 208 GVGGRGLQVHTVVVKNGLDKTIPVSNSLINLYLKCGNVRKARILFDKTE-VKSVVTWNSM 267
Query: 205 LAAYLLRSQEDLAFKLLIDMQEHGFEPDLYSYTSIISACFNENISNNGRSLHGLVIKRGF 264
++ Y + A + M+ + S+ S+I C N LH V+K GF
Sbjct: 268 ISGYAANGLDLEALGMFYSMRLNYVRLSESSFASVIKLCANLKELRFTEQLHCSVVKYGF 327
Query: 265 EQSVPISNALISMYLKSDYGSMKEALCIFESLE-FKDRVSWNSILTGLSQTGSSEDAVKS 324
I AL+ Y K +M +AL +F+ + + VSW ++++G Q E+AV
Sbjct: 328 LFDQNIRTALMVAYSKCT--AMLDALRLFKEIGCVGNVVSWTAMISGFLQNDGKEEAVDL 387
Query: 325 FLHMRSAAMDIDHYSFSAVLRSCSDLATFQLGQQIHVLALKYGLESNEFVSSSLIFMYSK 384
F M+ + + +++S +L + ++ ++H +K E + V ++L+ Y K
Sbjct: 388 FSEMKRKGVRPNEFTYSVILTALPVISP----SEVHAQVVKTNYERSSTVGTALLDAYVK 447
Query: 385 CGIIEDARRSFEEASKNSSITWNALMFGYAQHGQCNVALDLFFLMEEKKVKMDHITFVAV 444
G +E+A + F + W+A++ GYAQ G+ A+ +F + + +K + TF ++
Sbjct: 448 LGKVEEAAKVFSGIDDKDIVAWSAMLAGYAQTGETEAAIKMFGELTKGGIKPNEFTFSSI 507
Query: 445 LTACSHIGL-VEQGCKFLRCMESDYGVPPRMEHYACA----VDLYGRSGRLEEAKALIEE 504
L C+ + QG +F + + R++ C + +Y + G +E A+ + +
Sbjct: 508 LNVCAATNASMGQGKQF-----HGFAIKSRLDSSLCVSSALLTMYAKKGNIESAEEVFKR 567
Query: 505 MPFKPDTTVWKTFLGACRSCGNIELACQV 528
K D W + + G A V
Sbjct: 568 QREK-DLVSWNSMISGYAQHGQAMKALDV 583
HSP 3 Score: 189.5 bits (480), Expect = 1.1e-46
Identity = 122/464 (26.29%), Postives = 241/464 (51.94%), Query Frame = 1
Query: 80 KLEDAYLSFLSISKHNTVSWNAMINGYAQAGDRETAFWLLDCMEQEGEKVDDGTYAPLLP 139
+L +A+ F + S+ +++ G+++ G + A L + + G ++D ++ +L
Sbjct: 42 RLYNAHNLFDKSPGRDRESYISLLFGFSRDGRTQEAKRLFLNIHRLGMEMDCSIFSSVLK 101
Query: 140 LLDDADFCN--LTSQLHGKIIKHGLELVNTMCNALITSYSKCGSLDDAKRIFDSSAGIRD 199
+ A C+ QLH + IK G ++ +L+ +Y K + D +++FD R+
Sbjct: 102 V--SATLCDELFGRQLHCQCIKFGFLDDVSVGTSLVDTYMKGSNFKDGRKVFDEMKE-RN 161
Query: 200 LVTWNSLLAAYLLRSQEDLAFKLLIDMQEHGFEPDLYSYTSIISACFNENISNNGRSLHG 259
+VTW +L++ Y S D L + MQ G +P+ +++ + + E + G +H
Sbjct: 162 VVTWTTLISGYARNSMNDEVLTLFMRMQNEGTQPNSFTFAAALGVLAEEGVGGRGLQVHT 221
Query: 260 LVIKRGFEQSVPISNALISMYLKSDYGSMKEALCIFESLEFKDRVSWNSILTGLSQTGSS 319
+V+K G ++++P+SN+LI++YLK G++++A +F+ E K V+WNS+++G + G
Sbjct: 222 VVVKNGLDKTIPVSNSLINLYLKC--GNVRKARILFDKTEVKSVVTWNSMISGYAANGLD 281
Query: 320 EDAVKSFLHMRSAAMDIDHYSFSAVLRSCSDLATFQLGQQIHVLALKYGLESNEFVSSSL 379
+A+ F MR + + SF++V++ C++L + +Q+H +KYG ++ + ++L
Sbjct: 282 LEALGMFYSMRLNYVRLSESSFASVIKLCANLKELRFTEQLHCSVVKYGFLFDQNIRTAL 341
Query: 380 IFMYSKCGIIEDARRSFEE-ASKNSSITWNALMFGYAQHGQCNVALDLFFLMEEKKVKMD 439
+ YSKC + DA R F+E + ++W A++ G+ Q+ A+DLF M+ K V+ +
Sbjct: 342 MVAYSKCTAMLDALRLFKEIGCVGNVVSWTAMISGFLQNDGKEEAVDLFSEMKRKGVRPN 401
Query: 440 HITFVAVLTACSHIGLVEQGCKFLRCMESDYGVPPRMEHYACAVDLYGRSGRLEEAKALI 499
T+ +LTA I E + ++ ++Y + +D Y + G++EEA +
Sbjct: 402 EFTYSVILTALPVISPSEVHAQVVK---TNYERSSTVG--TALLDAYVKLGKVEEAAKVF 461
Query: 500 EEMPFKPDTTVWKTFLGACRSCGNIELACQVAGHLLE--MEPEE 539
+ K D W L G E A ++ G L + ++P E
Sbjct: 462 SGIDDK-DIVAWSAMLAGYAQTGETEAAIKMFGELTKGGIKPNE 494
BLAST of Cucsa.162960 vs. Swiss-Prot
Match:
PP333_ARATH (Pentatricopeptide repeat-containing protein At4g21300 OS=Arabidopsis thaliana GN=PCMP-E36 PE=3 SV=1)
HSP 1 Score: 390.6 bits (1002), Expect = 3.2e-107
Identity = 220/616 (35.71%), Postives = 340/616 (55.19%), Query Frame = 1
Query: 1 MIAGHINCGNLEASWDVLRCMRSCGFELDRYTFGSMLKGIAFAGMFHLGQQVHSIIIKMG 60
M+ G+ CG L++ MR + TF +L A + LG Q+H +++ G
Sbjct: 210 MLNGYAKCGALDSVIKGFSVMRMDQISPNAVTFDCVLSVCASKLLIDLGVQLHGLVVVSG 269
Query: 61 YAENVYAGSALLDMYAKCEKLEDAYLSFLSISKHNTVSWNAMINGYAQAGDRETAFWLLD 120
++LL MY+KC + +DA F +S+ +TV+WN MI+GY Q+G E +
Sbjct: 270 VDFEGSIKNSLLSMYSKCGRFDDASKLFRMMSRADTVTWNCMISGYVQSGLMEESLTFFY 329
Query: 121 CMEQEGEKVDDGTYAPLLPLLDDADFCNLTSQLHGKIIKHGLELVNTMCNALITSYSKCG 180
M G D T++ LLP + + Q+H I++H + L + +ALI +Y KC
Sbjct: 330 EMISSGVLPDAITFSSLLPSVSKFENLEYCKQIHCYIMRHSISLDIFLTSALIDAYFKCR 389
Query: 181 SLDDAKRIFDSSAGIRDLVTWNSLLAAYL---LRSQEDLAFKLLIDMQEHGFEPDLYSYT 240
+ A+ IF + D+V + ++++ YL L F+ L+ ++ E L S
Sbjct: 390 GVSMAQNIFSQCNSV-DVVVFTAMISGYLHNGLYIDSLEMFRWLVKVKISPNEITLVSIL 449
Query: 241 SIISACFNENISNNGRSLHGLVIKRGFEQSVPISNALISMYLKSDYGSMKEALCIFESLE 300
+I + GR LHG +IK+GF+ I A+I MY K G M A IFE L
Sbjct: 450 PVIGILLALKL---GRELHGFIIKKGFDNRCNIGCAVIDMYAKC--GRMNLAYEIFERLS 509
Query: 301 FKDRVSWNSILTGLSQTGSSEDAVKSFLHMRSAAMDIDHYSFSAVLRSCSDLATFQLGQQ 360
+D VSWNS++T +Q+ + A+ F M + + D S SA L +C++L + G+
Sbjct: 510 KRDIVSWNSMITRCAQSDNPSAAIDIFRQMGVSGICYDCVSISAALSACANLPSESFGKA 569
Query: 361 IHVLALKYGLESNEFVSSSLIFMYSKCGIIEDARRSFEEASKNSSITWNALMFGYAQHGQ 420
IH +K+ L S+ + S+LI MY+KCG ++ A F+ + + ++WN+++ HG+
Sbjct: 570 IHGFMIKHSLASDVYSESTLIDMYAKCGNLKAAMNVFKTMKEKNIVSWNSIIAACGNHGK 629
Query: 421 CNVALDLFFLMEEKK-VKMDHITFVAVLTACSHIGLVEQGCKFLRCMESDYGVPPRMEHY 480
+L LF M EK ++ D ITF+ ++++C H+G V++G +F R M DYG+ P+ EHY
Sbjct: 630 LKDSLCLFHEMVEKSGIRPDQITFLEIISSCCHVGDVDEGVRFFRSMTEDYGIQPQQEHY 689
Query: 481 ACAVDLYGRSGRLEEAKALIEEMPFKPDTTVWKTFLGACRSCGNIELACQVAGHLLEMEP 540
AC VDL+GR+GRL EA ++ MPF PD VW T LGACR N+ELA + L++++P
Sbjct: 690 ACVVDLFGRAGRLTEAYETVKSMPFPPDAGVWGTLLGACRLHKNVELAEVASSKLMDLDP 749
Query: 541 EEHCTYVLLSNMYGNLMRWDEKAKVKRLMKERGVKKVPGWSWIEVNNNVHAFIAQDHSHP 600
YVL+SN + N W+ KV+ LMKER V+K+PG+SWIE+N H F++ D +HP
Sbjct: 750 SNSGYYVLISNAHANAREWESVTKVRSLMKEREVQKIPGYSWIEINKRTHLFVSGDVNHP 809
Query: 601 SCQQIYFLLEVLLEEI 613
IY LL LL E+
Sbjct: 810 ESSHIYSLLNSLLGEL 819
HSP 2 Score: 167.5 bits (423), Expect = 4.5e-40
Identity = 117/502 (23.31%), Postives = 233/502 (46.41%), Query Frame = 1
Query: 25 GFELDRYTFGSMLKGIA----FAGMFHLGQQVHSIIIKMGYAENVYAGSALLDMYAKCEK 84
G D TF ++K F G+ L V S+ G N + S+L+ Y + K
Sbjct: 133 GVSPDVSTFPCLVKACVALKNFKGIDFLSDTVSSL----GMDCNEFVASSLIKAYLEYGK 192
Query: 85 LEDAYLSFLSISKHNTVSWNAMINGYAQAGDRETAFWLLDCMEQEGEKVDDGTYAPLLPL 144
++ F + + + V WN M+NGYA+ G ++ M + + T+ +L +
Sbjct: 193 IDVPSKLFDRVLQKDCVIWNVMLNGYAKCGALDSVIKGFSVMRMDQISPNAVTFDCVLSV 252
Query: 145 LDDADFCNLTSQLHGKIIKHGLELVNTMCNALITSYSKCGSLDDAKRIFDSSAGIRDLVT 204
+L QLHG ++ G++ ++ N+L++ YSKCG DDA ++F + D VT
Sbjct: 253 CASKLLIDLGVQLHGLVVVSGVDFEGSIKNSLLSMYSKCGRFDDASKLFRMMSRA-DTVT 312
Query: 205 WNSLLAAYLLRSQEDLAFKLLIDMQEHGFEPDLYSYTSIISACFNENISNNGRSLHGLVI 264
WN +++ Y+ + + +M G PD +++S++ + + +H ++
Sbjct: 313 WNCMISGYVQSGLMEESLTFFYEMISSGVLPDAITFSSLLPSVSKFENLEYCKQIHCYIM 372
Query: 265 KRGFEQSVPISNALISMYLKSDYGSMKEALCIFESLEFKDRVSWNSILTGLSQTGSSEDA 324
+ + +++ALI Y K SM + IF D V + ++++G G D+
Sbjct: 373 RHSISLDIFLTSALIDAYFKCRGVSMAQN--IFSQCNSVDVVVFTAMISGYLHNGLYIDS 432
Query: 325 VKSFLHMRSAAMDIDHYSFSAVLRSCSDLATFQLGQQIHVLALKYGLESNEFVSSSLIFM 384
++ F + + + + ++L L +LG+++H +K G ++ + ++I M
Sbjct: 433 LEMFRWLVKVKISPNEITLVSILPVIGILLALKLGRELHGFIIKKGFDNRCNIGCAVIDM 492
Query: 385 YSKCGIIEDARRSFEEASKNSSITWNALMFGYAQHGQCNVALDLFFLMEEKKVKMDHITF 444
Y+KCG + A FE SK ++WN+++ AQ + A+D+F M + D ++
Sbjct: 493 YAKCGRMNLAYEIFERLSKRDIVSWNSMITRCAQSDNPSAAIDIFRQMGVSGICYDCVSI 552
Query: 445 VAVLTACSHIGLVEQGCKFLRCMESDYGVPPRMEHYACAVDLYGRSGRLEEAKALIEEMP 504
A L+AC+++ G K + + + + + +D+Y + G L+ A + + M
Sbjct: 553 SAALSACANLPSESFG-KAIHGFMIKHSLASDVYSESTLIDMYAKCGNLKAAMNVFKTMK 612
Query: 505 FKPDTTVWKTFLGACRSCGNIE 523
K + W + + AC + G ++
Sbjct: 613 EK-NIVSWNSIIAACGNHGKLK 625
HSP 3 Score: 164.9 bits (416), Expect = 2.9e-39
Identity = 119/494 (24.09%), Postives = 223/494 (45.14%), Query Frame = 1
Query: 36 MLKGIAFAGMFHLGQQVHSIIIKMGYAENVYAGSALLDMYAKCEKLEDAYLSF--LSISK 95
+L+ + + G+QVH+ +I + + Y +L MYA C D F L + +
Sbjct: 41 LLQACSNPNLLRQGKQVHAFLIVNSISGDSYTDERILGMYAMCGSFSDCGKMFYRLDLRR 100
Query: 96 HNTVSWNAMINGYAQAGDRETAFWLLDCMEQEGEKVDDGTYAPLLPLLDDADFCNLTSQL 155
+ WN++I+ + + G A M G D T+ L+ L
Sbjct: 101 SSIRPWNSIISSFVRNGLLNQALAFYFKMLCFGVSPDVSTFPCLVKACVALKNFKGIDFL 160
Query: 156 HGKIIKHGLELVNTMCNALITSYSKCGSLDDAKRIFDSSAGIRDLVTWNSLLAAYLLRSQ 215
+ G++ + ++LI +Y + G +D ++FD +D V WN +L Y
Sbjct: 161 SDTVSSLGMDCNEFVASSLIKAYLEYGKIDVPSKLFDRVLQ-KDCVIWNVMLNGYAKCGA 220
Query: 216 EDLAFKLLIDMQEHGFEPDLYSYTSIISACFNENISNNGRSLHGLVIKRGFEQSVPISNA 275
D K M+ P+ ++ ++S C ++ + + G LHGLV+ G + I N+
Sbjct: 221 LDSVIKGFSVMRMDQISPNAVTFDCVLSVCASKLLIDLGVQLHGLVVVSGVDFEGSIKNS 280
Query: 276 LISMYLKSDYGSMKEALCIFESLEFKDRVSWNSILTGLSQTGSSEDAVKSFLHMRSAAMD 335
L+SMY K G +A +F + D V+WN +++G Q+G E+++ F M S+ +
Sbjct: 281 LLSMYSKC--GRFDDASKLFRMMSRADTVTWNCMISGYVQSGLMEESLTFFYEMISSGVL 340
Query: 336 IDHYSFSAVLRSCSDLATFQLGQQIHVLALKYGLESNEFVSSSLIFMYSKCGIIEDARRS 395
D +FS++L S S + +QIH +++ + + F++S+LI Y KC + A+
Sbjct: 341 PDAITFSSLLPSVSKFENLEYCKQIHCYIMRHSISLDIFLTSALIDAYFKCRGVSMAQNI 400
Query: 396 FEEASKNSSITWNALMFGYAQHGQCNVALDLFFLMEEKKVKMDHITFVAVLTACSHIGLV 455
F + + + + A++ GY +G +L++F + + K+ + IT V++L + +
Sbjct: 401 FSQCNSVDVVVFTAMISGYLHNGLYIDSLEMFRWLVKVKISPNEITLVSILPVIGILLAL 460
Query: 456 EQGCKFLRCMESDYGVPPRMEHYACAVDLYGRSGRLEEAKALIEEMPFKPDTTVWKTFLG 515
+ G + L G R +D+Y + GR+ A + E + K D W + +
Sbjct: 461 KLG-RELHGFIIKKGFDNRCNIGCAVIDMYAKCGRMNLAYEIFERLS-KRDIVSWNSMIT 520
Query: 516 ACRSCGNIELACQV 528
C N A +
Sbjct: 521 RCAQSDNPSAAIDI 529
BLAST of Cucsa.162960 vs. Swiss-Prot
Match:
PP151_ARATH (Pentatricopeptide repeat-containing protein At2g13600 OS=Arabidopsis thaliana GN=PCMP-E76 PE=3 SV=1)
HSP 1 Score: 379.4 bits (973), Expect = 7.5e-104
Identity = 218/629 (34.66%), Postives = 342/629 (54.37%), Query Frame = 1
Query: 25 GFELDRYTFGSMLKGIAFAGMFHLGQQVHSIIIKMGYAENVYAGSALLDMYAKCEKLEDA 84
GF + + ++ + G G+QV + + N+Y ++++ K L++A
Sbjct: 50 GFSNEIFIQNRLIDAYSKCGSLEDGRQVFDKMPQ----RNIYTWNSVVTGLTKLGFLDEA 109
Query: 85 YLSFLSISKHNTVSWNAMINGYAQAGDRETAFWLLDCMEQEGEKVDDGTYAPLLPLLDDA 144
F S+ + + +WN+M++G+AQ E A M +EG +++ ++A +L
Sbjct: 110 DSLFRSMPERDQCTWNSMVSGFAQHDRCEEALCYFAMMHKEGFVLNEYSFASVLSACSGL 169
Query: 145 DFCNLTSQLHGKIIKHGLELVNTMCNALITSYSKCGSLDDAKRIFDSSAGIRDLVTWNSL 204
+ N Q+H I K + +AL+ YSKCG+++DA+R+FD G R++V+WNSL
Sbjct: 170 NDMNKGVQVHSLIAKSPFLSDVYIGSALVDMYSKCGNVNDAQRVFDEM-GDRNVVSWNSL 229
Query: 205 LAAYLLRSQEDLAFKLLIDMQEHGFEPDLYSYTSIISACFNENISNNGRSLHGLVIK--- 264
+ + A + M E EPD + S+ISAC + + G+ +HG V+K
Sbjct: 230 ITCFEQNGPAVEALDVFQMMLESRVEPDEVTLASVISACASLSAIKVGQEVHGRVVKNDK 289
Query: 265 ------------------------RGFEQSVPISNALISMYLKSDYG---SMKEALCIFE 324
R S+PI N + + S Y S K A +F
Sbjct: 290 LRNDIILSNAFVDMYAKCSRIKEARFIFDSMPIRNVIAETSMISGYAMAASTKAARLMFT 349
Query: 325 SLEFKDRVSWNSILTGLSQTGSSEDAVKSFLHMRSAAMDIDHYSFSAVLRSCSDLATFQL 384
+ ++ VSWN+++ G +Q G +E+A+ F ++ ++ HYSF+ +L++C+DLA L
Sbjct: 350 KMAERNVVSWNALIAGYTQNGENEEALSLFCLLKRESVCPTHYSFANILKACADLAELHL 409
Query: 385 GQQIHVLALKYGL------ESNEFVSSSLIFMYSKCGIIEDARRSFEEASKNSSITWNAL 444
G Q HV LK+G E + FV +SLI MY KCG +E+ F + + ++WNA+
Sbjct: 410 GMQAHVHVLKHGFKFQSGEEDDIFVGNSLIDMYVKCGCVEEGYLVFRKMMERDCVSWNAM 469
Query: 445 MFGYAQHGQCNVALDLFFLMEEKKVKMDHITFVAVLTACSHIGLVEQGCKFLRCMESDYG 504
+ G+AQ+G N AL+LF M E K DHIT + VL+AC H G VE+G + M D+G
Sbjct: 470 IIGFAQNGYGNEALELFREMLESGEKPDHITMIGVLSACGHAGFVEEGRHYFSSMTRDFG 529
Query: 505 VPPRMEHYACAVDLYGRSGRLEEAKALIEEMPFKPDTTVWKTFLGACRSCGNIELACQVA 564
V P +HY C VDL GR+G LEEAK++IEEMP +PD+ +W + L AC+ NI L VA
Sbjct: 530 VAPLRDHYTCMVDLLGRAGFLEEAKSMIEEMPMQPDSVIWGSLLAACKVHRNITLGKYVA 589
Query: 565 GHLLEMEPEEHCTYVLLSNMYGNLMRWDEKAKVKRLMKERGVKKVPGWSWIEVNNNVHAF 618
LLE+EP YVLLSNMY L +W++ V++ M++ GV K PG SWI++ + H F
Sbjct: 590 EKLLEVEPSNSGPYVLLSNMYAELGKWEDVMNVRKSMRKEGVTKQPGCSWIKIQGHDHVF 649
HSP 2 Score: 96.3 bits (238), Expect = 1.3e-18
Identity = 63/214 (29.44%), Postives = 101/214 (47.20%), Query Frame = 1
Query: 1 MIAGHINCGNLEASWDVLRCMRSCGFELDRYTFGSMLKGIAFAGMFHLGQQVHSIIIKMG 60
+IAG+ G E + + ++ Y+F ++LK A HLG Q H ++K G
Sbjct: 357 LIAGYTQNGENEEALSLFCLLKRESVCPTHYSFANILKACADLAELHLGMQAHVHVLKHG 416
Query: 61 YA------ENVYAGSALLDMYAKCEKLEDAYLSFLSISKHNTVSWNAMINGYAQAGDRET 120
+ ++++ G++L+DMY KC +E+ YL F + + + VSWNAMI G+AQ G
Sbjct: 417 FKFQSGEEDDIFVGNSLIDMYVKCGCVEEGYLVFRKMMERDCVSWNAMIIGFAQNGYGNE 476
Query: 121 AFWLLDCMEQEGEKVDDGTYAPLLPLLDDADFCNLTSQLHGKIIK-HGLELVNTMCNALI 180
A L M + GEK D T +L A F + + G+ + ++
Sbjct: 477 ALELFREMLESGEKPDHITMIGVLSACGHAGFVEEGRHYFSSMTRDFGVAPLRDHYTCMV 536
Query: 181 TSYSKCGSLDDAKRIFDSSAGIRDLVTWNSLLAA 208
+ G L++AK + + D V W SLLAA
Sbjct: 537 DLLGRAGFLEEAKSMIEEMPMQPDSVIWGSLLAA 570
HSP 3 Score: 69.3 bits (168), Expect = 1.7e-10
Identity = 57/216 (26.39%), Postives = 100/216 (46.30%), Query Frame = 1
Query: 320 AVKSFLHMRSAAMDI-DHYSFSAVLRSC--SDLATFQLGQQIHVLALKYGLESNEFVSSS 379
A KSFL + + D F+ +L SC S L+ + + +H +K G + F+ +
Sbjct: 2 ATKSFLKLAADLSSFTDSSPFAKLLDSCIKSKLSAIYV-RYVHASVIKSGFSNEIFIQNR 61
Query: 380 LIFMYSKCGIIEDARRSFEEASKNSSITWNALMFGYAQHGQCNVALDLFFLMEEKKVKMD 439
LI YSKCG +ED R+ F++ + + TWN+++ G + G + A LF M E+ D
Sbjct: 62 LIDAYSKCGSLEDGRQVFDKMPQRNIYTWNSVVTGLTKLGFLDEADSLFRSMPER----D 121
Query: 440 HITFVAVLTACSHIGLVEQGCKFLRCMESDYGVPPRMEHYACAVDLYGRSGRLE-----E 499
T+ ++++ + E+ + M + V + Y+ A L SG + +
Sbjct: 122 QCTWNSMVSGFAQHDRCEEALCYFAMMHKEGFV---LNEYSFASVLSACSGLNDMNKGVQ 181
Query: 500 AKALIEEMPFKPDTTVWKTFLGACRSCGNIELACQV 528
+LI + PF D + + CGN+ A +V
Sbjct: 182 VHSLIAKSPFLSDVYIGSALVDMYSKCGNVNDAQRV 209
HSP 4 Score: 54.7 bits (130), Expect = 4.2e-06
Identity = 63/314 (20.06%), Postives = 128/314 (40.76%), Query Frame = 1
Query: 232 DLYSYTSIISACFNENISN-NGRSLHGLVIKRGFEQSVPISNALISMYLKSDYGSMKEAL 291
D + ++ +C +S R +H VIK GF + I N LI Y K GS+++
Sbjct: 18 DSSPFAKLLDSCIKSKLSAIYVRYVHASVIKSGFSNEIFIQNRLIDAYSKC--GSLEDGR 77
Query: 292 CIFESLEFKDRVSWNSILTGLSQTGSSEDAVKSFLHMRSAAMDIDHYSFSAVLRSCSDLA 351
+F+ + ++ +WNS++TGL++ G ++A F M + D ++++++ +
Sbjct: 78 QVFDKMPQRNIYTWNSVVTGLTKLGFLDEADSLFRSM----PERDQCTWNSMVSGFAQHD 137
Query: 352 TFQLGQQIHVLALKYGLESNEFVSSSLIFMYSKCGIIEDARRSFEEASKNSSITW----- 411
+ + K G NE+ +S++ S C + D + + S + +
Sbjct: 138 RCEEALCYFAMMHKEGFVLNEYSFASVL---SACSGLNDMNKGVQVHSLIAKSPFLSDVY 197
Query: 412 --NALMFGYAQHGQCNVALDLFFLMEEKKVKMDHITFVAVLTACSHIGLVEQGCKFLRCM 471
+AL+ Y++ G N A +F M ++ V +++ +++T G + + M
Sbjct: 198 IGSALVDMYSKCGNVNDAQRVFDEMGDRNV----VSWNSLITCFEQNGPAVEALDVFQMM 257
Query: 472 ESDYGVPPRME----HYACA-------------------------------VDLYGRSGR 503
P + ACA VD+Y + R
Sbjct: 258 LESRVEPDEVTLASVISACASLSAIKVGQEVHGRVVKNDKLRNDIILSNAFVDMYAKCSR 317
BLAST of Cucsa.162960 vs. TrEMBL
Match:
A0A0A0LMK6_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_2G382810 PE=4 SV=1)
HSP 1 Score: 1260.7 bits (3261), Expect = 0.0e+00
Identity = 619/619 (100.00%), Postives = 619/619 (100.00%), Query Frame = 1
Query: 1 MIAGHINCGNLEASWDVLRCMRSCGFELDRYTFGSMLKGIAFAGMFHLGQQVHSIIIKMG 60
MIAGHINCGNLEASWDVLRCMRSCGFELDRYTFGSMLKGIAFAGMFHLGQQVHSIIIKMG
Sbjct: 70 MIAGHINCGNLEASWDVLRCMRSCGFELDRYTFGSMLKGIAFAGMFHLGQQVHSIIIKMG 129
Query: 61 YAENVYAGSALLDMYAKCEKLEDAYLSFLSISKHNTVSWNAMINGYAQAGDRETAFWLLD 120
YAENVYAGSALLDMYAKCEKLEDAYLSFLSISKHNTVSWNAMINGYAQAGDRETAFWLLD
Sbjct: 130 YAENVYAGSALLDMYAKCEKLEDAYLSFLSISKHNTVSWNAMINGYAQAGDRETAFWLLD 189
Query: 121 CMEQEGEKVDDGTYAPLLPLLDDADFCNLTSQLHGKIIKHGLELVNTMCNALITSYSKCG 180
CMEQEGEKVDDGTYAPLLPLLDDADFCNLTSQLHGKIIKHGLELVNTMCNALITSYSKCG
Sbjct: 190 CMEQEGEKVDDGTYAPLLPLLDDADFCNLTSQLHGKIIKHGLELVNTMCNALITSYSKCG 249
Query: 181 SLDDAKRIFDSSAGIRDLVTWNSLLAAYLLRSQEDLAFKLLIDMQEHGFEPDLYSYTSII 240
SLDDAKRIFDSSAGIRDLVTWNSLLAAYLLRSQEDLAFKLLIDMQEHGFEPDLYSYTSII
Sbjct: 250 SLDDAKRIFDSSAGIRDLVTWNSLLAAYLLRSQEDLAFKLLIDMQEHGFEPDLYSYTSII 309
Query: 241 SACFNENISNNGRSLHGLVIKRGFEQSVPISNALISMYLKSDYGSMKEALCIFESLEFKD 300
SACFNENISNNGRSLHGLVIKRGFEQSVPISNALISMYLKSDYGSMKEALCIFESLEFKD
Sbjct: 310 SACFNENISNNGRSLHGLVIKRGFEQSVPISNALISMYLKSDYGSMKEALCIFESLEFKD 369
Query: 301 RVSWNSILTGLSQTGSSEDAVKSFLHMRSAAMDIDHYSFSAVLRSCSDLATFQLGQQIHV 360
RVSWNSILTGLSQTGSSEDAVKSFLHMRSAAMDIDHYSFSAVLRSCSDLATFQLGQQIHV
Sbjct: 370 RVSWNSILTGLSQTGSSEDAVKSFLHMRSAAMDIDHYSFSAVLRSCSDLATFQLGQQIHV 429
Query: 361 LALKYGLESNEFVSSSLIFMYSKCGIIEDARRSFEEASKNSSITWNALMFGYAQHGQCNV 420
LALKYGLESNEFVSSSLIFMYSKCGIIEDARRSFEEASKNSSITWNALMFGYAQHGQCNV
Sbjct: 430 LALKYGLESNEFVSSSLIFMYSKCGIIEDARRSFEEASKNSSITWNALMFGYAQHGQCNV 489
Query: 421 ALDLFFLMEEKKVKMDHITFVAVLTACSHIGLVEQGCKFLRCMESDYGVPPRMEHYACAV 480
ALDLFFLMEEKKVKMDHITFVAVLTACSHIGLVEQGCKFLRCMESDYGVPPRMEHYACAV
Sbjct: 490 ALDLFFLMEEKKVKMDHITFVAVLTACSHIGLVEQGCKFLRCMESDYGVPPRMEHYACAV 549
Query: 481 DLYGRSGRLEEAKALIEEMPFKPDTTVWKTFLGACRSCGNIELACQVAGHLLEMEPEEHC 540
DLYGRSGRLEEAKALIEEMPFKPDTTVWKTFLGACRSCGNIELACQVAGHLLEMEPEEHC
Sbjct: 550 DLYGRSGRLEEAKALIEEMPFKPDTTVWKTFLGACRSCGNIELACQVAGHLLEMEPEEHC 609
Query: 541 TYVLLSNMYGNLMRWDEKAKVKRLMKERGVKKVPGWSWIEVNNNVHAFIAQDHSHPSCQQ 600
TYVLLSNMYGNLMRWDEKAKVKRLMKERGVKKVPGWSWIEVNNNVHAFIAQDHSHPSCQQ
Sbjct: 610 TYVLLSNMYGNLMRWDEKAKVKRLMKERGVKKVPGWSWIEVNNNVHAFIAQDHSHPSCQQ 669
Query: 601 IYFLLEVLLEEITRMEDAD 620
IYFLLEVLLEEITRMEDAD
Sbjct: 670 IYFLLEVLLEEITRMEDAD 688
BLAST of Cucsa.162960 vs. TrEMBL
Match:
A0A0A0LMK6_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_2G382810 PE=4 SV=1)
HSP 1 Score: 126.3 bits (316), Expect = 1.3e-25
Identity = 76/270 (28.15%), Postives = 132/270 (48.89%), Query Frame = 1
Query: 256 HGLVIKRGFEQSVPISNALISMYLKSDYGSMKEALCIFESLEFKDRVSWNSILTGLSQTG 315
H L +K G V N +++ Y K ++ A +F+ + +D VSWN+++ G G
Sbjct: 21 HSLAVKLGTIADVYTCNNILNGYWKCK--ELRSADVLFDEMPMRDSVSWNTMIAGHINCG 80
Query: 316 SSEDAVKSFLHMRSAAMDIDHYSFSAVLRSCSDLATFQLGQQIHVLALKYGLESNEFVSS 375
+ E + MRS ++D Y+F ++L+ + F LGQQ+H + +K G N + S
Sbjct: 81 NLEASWDVLRCMRSCGFELDRYTFGSMLKGIAFAGMFHLGQQVHSIIIKMGYAENVYAGS 140
Query: 376 SLIFMYSKCGIIEDARRSFEEASKNSSITWNALMFGYAQHGQCNVALDLFFLMEEKKVKM 435
+L+ MY+KC +EDA SF SK+++++WNA++ GYAQ G A L ME++ K+
Sbjct: 141 ALLDMYAKCEKLEDAYLSFLSISKHNTVSWNAMINGYAQAGDRETAFWLLDCMEQEGEKV 200
Query: 436 DHITFVAV-----------LTACSHIGLVEQGCKFLRCMESDYGVPPRMEHYACAVDLYG 495
D T+ + LT+ H +++ G + + M + + Y
Sbjct: 201 DDGTYAPLLPLLDDADFCNLTSQLHGKIIKHGLELVNTMCN------------ALITSYS 260
Query: 496 RSGRLEEAKALIEEMPFKPDTTVWKTFLGA 515
+ G L++AK + + D W + L A
Sbjct: 261 KCGSLDDAKRIFDSSAGIRDLVTWNSLLAA 276
HSP 2 Score: 900.6 bits (2326), Expect = 1.1e-258
Identity = 426/619 (68.82%), Postives = 521/619 (84.17%), Query Frame = 1
Query: 1 MIAGHINCGNLEASWDVLRCMRSCGFELDRYTFGSMLKGIAFAGMFHLGQQVHSIIIKMG 60
MIAG++NCGNLE ++++++ M+ CGF+ D YTFGS+LKG+A A +GQQ+HS+I+KMG
Sbjct: 72 MIAGYVNCGNLETAFEIMKDMKRCGFDFDGYTFGSLLKGVASAYRLQVGQQLHSMIVKMG 131
Query: 61 YAENVYAGSALLDMYAKCEKLEDAYLSFLSISKHNTVSWNAMINGYAQAGDRETAFWLLD 120
Y ENVYAGSALLDMYAKCEK+ DAY+ F + + N+VSWNA+I G++Q GDR T FWLLD
Sbjct: 132 YEENVYAGSALLDMYAKCEKVGDAYMVFECLPEPNSVSWNALIAGFSQMGDRSTVFWLLD 191
Query: 121 CMEQEGEKVDDGTYAPLLPLLDDADFCNLTSQLHGKIIKHGLELVNTMCNALITSYSKCG 180
CME+EG KVDDGTYAPLL LLDD +F LT Q+HGKIIK GL NT+CNA+ITSYS+CG
Sbjct: 192 CMEKEGVKVDDGTYAPLLTLLDDIEFYKLTIQIHGKIIKRGLACDNTVCNAMITSYSECG 251
Query: 181 SLDDAKRIFDSSAGIRDLVTWNSLLAAYLLRSQEDLAFKLLIDMQEHGFEPDLYSYTSII 240
S+ DA+++FD + G+RDLVTWNS+LAAYL+ +E+L FKL +DMQ GFEPD+Y+YTSI+
Sbjct: 252 SIGDARKVFDDAVGMRDLVTWNSMLAAYLVHEKEELGFKLFLDMQRLGFEPDIYTYTSIL 311
Query: 241 SACFNENISNNGRSLHGLVIKRGFEQSVPISNALISMYLKSDYGSMKEALCIFESLEFKD 300
SACF + ++G+S+H +VIKRG E SVPISNALI+MYLKS+ SM+EAL +FES+E KD
Sbjct: 312 SACFEKAHKSHGKSVHAVVIKRGLEYSVPISNALIAMYLKSNSTSMEEALSLFESMELKD 371
Query: 301 RVSWNSILTGLSQTGSSEDAVKSFLHMRSAAMDIDHYSFSAVLRSCSDLATFQLGQQIHV 360
RVSWNSILTG SQ G SEDA+ F MR ++IDHY+ SAVLRSCSDLAT QLG+Q+HV
Sbjct: 372 RVSWNSILTGFSQIGLSEDALNFFGKMRGFMVEIDHYALSAVLRSCSDLATLQLGRQVHV 431
Query: 361 LALKYGLESNEFVSSSLIFMYSKCGIIEDARRSFEEASKNSSITWNALMFGYAQHGQCNV 420
LA+K G E+N+FV+S+LIFMYSKCGII+DAR+SFEE K+ SI WN+++FGYAQ+GQ N
Sbjct: 432 LAIKLGFETNDFVASALIFMYSKCGIIQDARKSFEETPKDISIAWNSIIFGYAQNGQGND 491
Query: 421 ALDLFFLMEEKKVKMDHITFVAVLTACSHIGLVEQGCKFLRCMESDYGVPPRMEHYACAV 480
ALDLFFLM + KV++DHITFVAVLTACSHIGLVE+G FL+ MESDYG+PPRMEHYACAV
Sbjct: 492 ALDLFFLMRDTKVRLDHITFVAVLTACSHIGLVEEGLNFLKSMESDYGIPPRMEHYACAV 551
Query: 481 DLYGRSGRLEEAKALIEEMPFKPDTTVWKTFLGACRSCGNIELACQVAGHLLEMEPEEHC 540
DL+GR+GRL+EAK LIE MPFKPD VWKT LGACR CG+IELA QVA HLL++EPEEHC
Sbjct: 552 DLFGRAGRLDEAKPLIESMPFKPDAMVWKTLLGACRVCGDIELAAQVASHLLDLEPEEHC 611
Query: 541 TYVLLSNMYGNLMRWDEKAKVKRLMKERGVKKVPGWSWIEVNNNVHAFIAQDHSHPSCQQ 600
TYV+LSNMYG+L RW EKA V RLM+ERGVKKVPGWSWIE+ N VHAF A+D SHP C++
Sbjct: 612 TYVILSNMYGHLRRWGEKASVTRLMRERGVKKVPGWSWIEIKNQVHAFNAEDQSHPHCKE 671
Query: 601 IYFLLEVLLEEITRMEDAD 620
IY +L L+EEIT + DAD
Sbjct: 672 IYQMLGGLMEEITWL-DAD 689
BLAST of Cucsa.162960 vs. TrEMBL
Match:
A0A061GHG1_THECC (Pentatricopeptide repeat-containing protein, putative OS=Theobroma cacao GN=TCM_036872 PE=4 SV=1)
HSP 1 Score: 212.2 bits (539), Expect = 1.8e-51
Identity = 141/486 (29.01%), Postives = 245/486 (50.41%), Query Frame = 1
Query: 30 RYTFGSMLKGIAFAGMFHLGQQVHSIIIKMGYAENVYAGSALLDMYAKCEKLEDAYLSFL 89
R S+L+ A+A F+ H IK+G +VY + +L+ YA+C++L A F
Sbjct: 2 RRPLNSLLESSAYA--FYKVLTTHCCAIKLGTLADVYTANKILNAYARCKELHVARKLFA 61
Query: 90 SISKHNTVSWNAMINGYAQAGDRETAFWLLDCMEQEGEKVDDGTYAPLLPLLDDADFCNL 149
+ +TVSWN MI GY G+ ETAF ++ M++ G D T+ LL + A +
Sbjct: 62 EVLHRDTVSWNTMIAGYVNCGNLETAFEIMKDMKRCGFDFDGYTFGSLLKGVASAYRLQV 121
Query: 150 TSQLHGKIIKHGLELVNTMCNALITSYSKCGSLDDAKRIFDSSAGIRDLVTWNSLLAAYL 209
QLH I+K G E +AL+ Y+KC + DA +F+ + V+WN+L+A +
Sbjct: 122 GQQLHSMIVKMGYEENVYAGSALLDMYAKCEKVGDAYMVFECLPE-PNSVSWNALIAGFS 181
Query: 210 LRSQEDLAFKLLIDMQEHGFEPDLYSYTSIISACFNENISNNGRSLHGLVIKRGFEQSVP 269
F LL M++ G + D +Y +++ + +HG +IKRG
Sbjct: 182 QMGDRSTVFWLLDCMEKEGVKVDDGTYAPLLTLLDDIEFYKLTIQIHGKIIKRGLACDNT 241
Query: 270 ISNALISMYLKSDYGSMKEALCIF-ESLEFKDRVSWNSILTGLSQTGSSEDAVKSFLHMR 329
+ NA+I+ Y S+ GS+ +A +F +++ +D V+WNS+L E K FL M+
Sbjct: 242 VCNAMITSY--SECGSIGDARKVFDDAVGMRDLVTWNSMLAAYLVHEKEELGFKLFLDMQ 301
Query: 330 SAAMDIDHYSFSAVLRSCSDLATFQLGQQIHVLALKYGLESNEFVSSSLIFMYSKCG--I 389
+ D Y+++++L +C + A G+ +H + +K GLE + +S++LI MY K
Sbjct: 302 RLGFEPDIYTYTSILSACFEKAHKSHGKSVHAVVIKRGLEYSVPISNALIAMYLKSNSTS 361
Query: 390 IEDARRSFEEASKNSSITWNALMFGYAQHGQCNVALDLFFLMEEKKVKMDHITFVAVLTA 449
+E+A FE ++WN+++ G++Q G AL+ F M V++DH AVL +
Sbjct: 362 MEEALSLFESMELKDRVSWNSILTGFSQIGLSEDALNFFGKMRGFMVEIDHYALSAVLRS 421
Query: 450 CSHIGLVEQGCKFLRCMESDYGVPPRMEHYACAVDLYGRSGRLEEAKALIEEMPFKPDTT 509
CS + ++ G + + + G + + +Y + G +++A+ EE P K +
Sbjct: 422 CSDLATLQLG-RQVHVLAIKLGFETNDFVASALIFMYSKCGIIQDARKSFEETP-KDISI 480
Query: 510 VWKTFL 513
W + +
Sbjct: 482 AWNSII 480
HSP 2 Score: 890.6 bits (2300), Expect = 1.1e-255
Identity = 429/616 (69.64%), Postives = 519/616 (84.25%), Query Frame = 1
Query: 1 MIAGHINCGNLEASWDVLRCMRSCGFELDRYTFGSMLKGIAFAGMFHLGQQVHSIIIKMG 60
MIAGH+N GN E +W++LR M+ CGF+ D YTFGS+LKG+A A LG+QVHS+++K G
Sbjct: 1 MIAGHVNYGNFETAWEILREMKGCGFDFDGYTFGSILKGVACAYRCDLGEQVHSMVVKTG 60
Query: 61 YAENVYAGSALLDMYAKCEKLEDAYLSFLSISKHNTVSWNAMINGYAQAGDRETAFWLLD 120
Y NVY+GSALLDMYAKC ++E+AY+ F S+ + N+VSWNA+I G+ Q GDR+TAFWL++
Sbjct: 61 YVGNVYSGSALLDMYAKCGRVEEAYVVFDSLPERNSVSWNALIAGFVQVGDRKTAFWLMN 120
Query: 121 CMEQEGEKVDDGTYAPLLPLLDDADFCNLTSQLHGKIIKHGLELVNTMCNALITSYSKCG 180
CMEQEG K+DDG AP+L LLDDA+F T Q+H KI+KHGL+ +T+CNA ITSYS+CG
Sbjct: 121 CMEQEGVKLDDGAIAPILTLLDDAEFYKSTMQIHCKILKHGLDCKSTVCNATITSYSECG 180
Query: 181 SLDDAKRIFDSSAGIRDLVTWNSLLAAYLLRSQEDLAFKLLIDMQEHGFEPDLYSYTSII 240
S++DA+ +FD + G RDLVTWNS+LAAYL+ +E LA KL IDMQ GFEPD+YSYTSII
Sbjct: 181 SIEDAETVFDGAFGSRDLVTWNSMLAAYLIHKKELLACKLFIDMQMLGFEPDIYSYTSII 240
Query: 241 SACFNENISNNGRSLHGLVIKRGFEQSVPISNALISMYLKSDYGSMKEALCIFESLEFKD 300
SA + N+G+S HGLVIKRG E+S PISNALI+ YLKS+ SM+EAL IF+S+E KD
Sbjct: 241 SAYSEDVHKNHGKSYHGLVIKRGLEKSEPISNALIARYLKSNSNSMEEALQIFKSMESKD 300
Query: 301 RVSWNSILTGLSQTGSSEDAVKSFLHMRSAAMDIDHYSFSAVLRSCSDLATFQLGQQIHV 360
RVSWNSILTGLSQ G +E A+K F HMR A +DIDHY+FSAVLRSCSDLAT QLGQ+IH
Sbjct: 301 RVSWNSILTGLSQMGLNEYALKFFGHMRYAEVDIDHYAFSAVLRSCSDLATLQLGQEIHA 360
Query: 361 LALKYGLESNEFVSSSLIFMYSKCGIIEDARRSFEEASKNSSITWNALMFGYAQHGQCNV 420
LA K GLESNEFV+SSLIFMY+KCGIIEDAR+SFE+ K+SSITWN+++FGYAQHGQ V
Sbjct: 361 LAHKSGLESNEFVASSLIFMYTKCGIIEDARKSFEQTPKDSSITWNSIIFGYAQHGQGYV 420
Query: 421 ALDLFFLMEEKKVKMDHITFVAVLTACSHIGLVEQGCKFLRCMESDYGVPPRMEHYACAV 480
ALDLFF M+++KVK+DHITFVAVLTACSH+GLVEQGCKFL MESDYG+ PRMEHYACAV
Sbjct: 421 ALDLFFQMKKEKVKLDHITFVAVLTACSHMGLVEQGCKFLMSMESDYGITPRMEHYACAV 480
Query: 481 DLYGRSGRLEEAKALIEEMPFKPDTTVWKTFLGACRSCGNIELACQVAGHLLEMEPEEHC 540
DLYGR+GRL+EAKALIE MPFKPD V +T LGACR+CGNIELA Q+AG+LLE+EPEEHC
Sbjct: 481 DLYGRAGRLDEAKALIEAMPFKPDAMVLRTLLGACRACGNIELASQIAGYLLELEPEEHC 540
Query: 541 TYVLLSNMYGNLMRWDEKAKVKRLMKERGVKKVPGWSWIEVNNNVHAFIAQDHSHPSCQQ 600
TYVLLS+MYG+L RWDEKA VKRLM+ERGV+KVPGWSWIE+ N V +F A+D SH C++
Sbjct: 541 TYVLLSDMYGHLKRWDEKASVKRLMRERGVRKVPGWSWIEIKNEVFSFKAEDRSHSHCEE 600
Query: 601 IYFLLEVLLEEITRME 617
IY +L VL+EEI RM+
Sbjct: 601 IYCVLGVLMEEIKRMD 616
BLAST of Cucsa.162960 vs. TrEMBL
Match:
W9S7C5_9ROSA (Uncharacterized protein OS=Morus notabilis GN=L484_007144 PE=4 SV=1)
HSP 1 Score: 887.9 bits (2293), Expect = 7.2e-255
Identity = 424/620 (68.39%), Postives = 510/620 (82.26%), Query Frame = 1
Query: 1 MIAGHINCGNLEASWDVLRCMRSCGFELDRYTFGSMLKGIAFAGMFHLGQQVHSIIIKMG 60
MIAG++N GN E +W+ + M+ CGFELD YTFGS+LKG+A A + +GQQVHS+I+KMG
Sbjct: 71 MIAGNVNRGNFENAWEFFKNMKKCGFELDGYTFGSLLKGVASAHQWSIGQQVHSMIVKMG 130
Query: 61 YAENVYAGSALLDMYAKCEKLEDAYLSFLSISKHNTVSWNAMINGYAQAGDRETAFWLLD 120
YAENVY GSALLDMYAKC ++EDA+L + + N VSWNA+I+GY Q GDR+TAF L
Sbjct: 131 YAENVYCGSALLDMYAKCGRVEDAFLVLEGMPERNPVSWNALISGYVQLGDRDTAFLLFA 190
Query: 121 CMEQEGEKVDDGTYAPLLPLLDDADFCNLTSQLHGKIIKHGLELVNTMCNALITSYSKCG 180
CMEQEG K++DGT APLL LLDDA+F T Q+HGK IKHGLE N +CNA ITSYS+CG
Sbjct: 191 CMEQEGLKIEDGTIAPLLTLLDDAEFYLSTMQMHGKAIKHGLEFENKVCNATITSYSECG 250
Query: 181 SLDDAKRIFDSSAGIRDLVTWNSLLAAYLLRSQEDLAFKLLIDMQEHGFEPDLYSYTSII 240
S+ DAK++FD S G RDLVTWNS+L AYL+ ++E+ AF L IDMQ GFEPD+YSYTSII
Sbjct: 251 SIADAKKVFDGSFGTRDLVTWNSMLGAYLVHNKEECAFNLFIDMQRFGFEPDIYSYTSII 310
Query: 241 SACFNENISNNGRSLHGLVIKRGFEQSVPISNALISMYLKSDYGSMKEALCIFESLEFKD 300
SACF E +G+SLHGL+IKRG EQSVP+ NALI+MYLKS+ SM E L IFES+EFKD
Sbjct: 311 SACFEEEHKKHGKSLHGLIIKRGLEQSVPVCNALIAMYLKSNTRSMVEPLSIFESMEFKD 370
Query: 301 RVSWNSILTGLSQTGSSEDAVKSFLHMRSAAMDIDHYSFSAVLRSCSDLATFQLGQQIHV 360
RVSWNSILTGLSQ G SEDA+K F HM+ A ++IDHYSFSAVLRSC+DLAT QLGQQ+HV
Sbjct: 371 RVSWNSILTGLSQVGLSEDALKFFGHMQFAILEIDHYSFSAVLRSCADLATLQLGQQVHV 430
Query: 361 LALKYGLESNEFVSSSLIFMYSKCGIIEDARRSFEEASKNSSITWNALMFGYAQHGQCNV 420
LA+K GL SNEFV SSLIFMY+KCGIIEDAR+SFEE K+SSITWN+++F YAQHGQ +
Sbjct: 431 LAIKSGLNSNEFVVSSLIFMYAKCGIIEDARKSFEENPKDSSITWNSIIFAYAQHGQGYI 490
Query: 421 ALDLFFLME-EKKVKMDHITFVAVLTACSHIGLVEQGCKFLRCMESDYGVPPRMEHYACA 480
ALD F M+ ++VK+DHITFVAVLTACSH+GLVE+GCK L+ ME +G+PPR+EHYACA
Sbjct: 491 ALDFFSQMKMREEVKLDHITFVAVLTACSHMGLVEEGCKLLKSMEFKHGIPPRVEHYACA 550
Query: 481 VDLYGRSGRLEEAKALIEEMPFKPDTTVWKTFLGACRSCGNIELACQVAGHLLEMEPEEH 540
VD+YGR+GRL+EAKAL+E MPF+PD VWKT L ACR+CGNIE A QVA HLL++EPEEH
Sbjct: 551 VDMYGRAGRLDEAKALVESMPFEPDAMVWKTLLSACRACGNIEFASQVASHLLDVEPEEH 610
Query: 541 CTYVLLSNMYGNLMRWDEKAKVKRLMKERGVKKVPGWSWIEVNNNVHAFIAQDHSHPSCQ 600
CTYV+LS++Y L RWDE A VKRLM++RGVKKVPGWSWIE+ N VHAF A+D HP+
Sbjct: 611 CTYVILSDLYRLLRRWDESASVKRLMRQRGVKKVPGWSWIEIKNEVHAFKAEDRLHPNSD 670
Query: 601 QIYFLLEVLLEEITRMEDAD 620
IYFLL V ++EI R++D D
Sbjct: 671 DIYFLLGVFMDEIRRLDDVD 690
BLAST of Cucsa.162960 vs. TrEMBL
Match:
W9S7C5_9ROSA (Uncharacterized protein OS=Morus notabilis GN=L484_007144 PE=4 SV=1)
HSP 1 Score: 120.9 bits (302), Expect = 5.3e-24
Identity = 72/264 (27.27%), Postives = 131/264 (49.62%), Query Frame = 1
Query: 256 HGLVIKRGFEQSVPISNALISMYLKSDYGSMKEALCIFESLEFKDRVSWNSILTGLSQTG 315
H IK G + I+N ++ Y + + L F+ + +D VSWN+++ G G
Sbjct: 22 HSWAIKSGTLSDIYIANNILCGYSRRQESWLAHKL--FDEMSQRDSVSWNTMIAGNVNRG 81
Query: 316 SSEDAVKSFLHMRSAAMDIDHYSFSAVLRSCSDLATFQLGQQIHVLALKYGLESNEFVSS 375
+ E+A + F +M+ ++D Y+F ++L+ + + +GQQ+H + +K G N + S
Sbjct: 82 NFENAWEFFKNMKKCGFELDGYTFGSLLKGVASAHQWSIGQQVHSMIVKMGYAENVYCGS 141
Query: 376 SLIFMYSKCGIIEDARRSFEEASKNSSITWNALMFGYAQHGQCNVALDLFFLMEEKKVKM 435
+L+ MY+KCG +EDA E + + ++WNAL+ GY Q G + A LF ME++ +K+
Sbjct: 142 ALLDMYAKCGRVEDAFLVLEGMPERNPVSWNALISGYVQLGDRDTAFLLFACMEQEGLKI 201
Query: 436 DHITFVAVLTACSHIGLVEQGCKFLRCMES-----DYGVPPRMEHYACAVDLYGRSGRLE 495
+ T +LT L++ +L M+ +G+ + + Y G +
Sbjct: 202 EDGTIAPLLT------LLDDAEFYLSTMQMHGKAIKHGLEFENKVCNATITSYSECGSIA 261
Query: 496 EAKALIEEMPFKPDTTVWKTFLGA 515
+AK + + D W + LGA
Sbjct: 262 DAKKVFDGSFGTRDLVTWNSMLGA 277
HSP 2 Score: 881.3 bits (2276), Expect = 6.7e-253
Identity = 415/616 (67.37%), Postives = 514/616 (83.44%), Query Frame = 1
Query: 1 MIAGHINCGNLEASWDVLRCMRSCGFELDRYTFGSMLKGIAFAGMFHLGQQVHSIIIKMG 60
MIAG +NCGNLE + +L+ MR C F+ D Y+FGS+LKG+A A +GQQ+HSI+IKMG
Sbjct: 72 MIAGFVNCGNLETACKILKNMRICDFDFDGYSFGSLLKGVASAYRLEVGQQLHSIVIKMG 131
Query: 61 YAENVYAGSALLDMYAKCEKLEDAYLSFLSISKHNTVSWNAMINGYAQAGDRETAFWLLD 120
Y ENVYAGSALLDMYAKCEK+EDAY F + + N+VSWNA+I G+++ GDR TAFWLL
Sbjct: 132 YEENVYAGSALLDMYAKCEKVEDAYTVFEYLPEPNSVSWNALIAGFSKVGDRSTAFWLLH 191
Query: 121 CMEQEGEKVDDGTYAPLLPLLDDADFCNLTSQLHGKIIKHGLELVNTMCNALITSYSKCG 180
CME+EG + +DGT+APLL LLDD +F LT Q+HGKI+KHGL NT+CNA+IT+YS+CG
Sbjct: 192 CMEKEGVRAEDGTFAPLLTLLDDIEFYKLTIQIHGKIVKHGLAFDNTVCNAMITAYSECG 251
Query: 181 SLDDAKRIFDSSAGIRDLVTWNSLLAAYLLRSQEDLAFKLLIDMQEHGFEPDLYSYTSII 240
S+ D +++FD + G+RDLVTWNS+LAAYL+ +E+L F+L +DMQ GFEPD+Y+YTSI+
Sbjct: 252 SIRDGRKVFDGAVGMRDLVTWNSMLAAYLVHEEEELGFQLFLDMQRLGFEPDIYTYTSIL 311
Query: 241 SACFNENISNNGRSLHGLVIKRGFEQSVPISNALISMYLKSDYGSMKEALCIFESLEFKD 300
S CF + ++G+SLH +VIKRG E VPISNALI+MYLKS+ SM EAL +FES+E KD
Sbjct: 312 SGCFEKAHKSHGQSLHAVVIKRGLEYLVPISNALIAMYLKSNNTSMGEALKLFESMELKD 371
Query: 301 RVSWNSILTGLSQTGSSEDAVKSFLHMRSAAMDIDHYSFSAVLRSCSDLATFQLGQQIHV 360
RVSWNSILTG SQ G +EDA+K F MRS ++IDHY+FSAVLRSC+DLAT QLG+Q+HV
Sbjct: 372 RVSWNSILTGFSQIGLNEDALKLFGQMRSLMVEIDHYAFSAVLRSCADLATLQLGRQVHV 431
Query: 361 LALKYGLESNEFVSSSLIFMYSKCGIIEDARRSFEEASKNSSITWNALMFGYAQHGQCNV 420
LA+K G E+N+FV+S+LIF+YSKCGIIEDAR+SFEE +SSI WN+L+FGYAQ+GQ ++
Sbjct: 432 LAIKSGFETNDFVASALIFLYSKCGIIEDARKSFEETPNDSSIAWNSLIFGYAQNGQGSI 491
Query: 421 ALDLFFLMEEKKVKMDHITFVAVLTACSHIGLVEQGCKFLRCMESDYGVPPRMEHYACAV 480
ALDLFFLM ++KV++DHITFVAVLTACSHIGLVE+G FL+ MESDYG+PPRMEHYACAV
Sbjct: 492 ALDLFFLMRDRKVRLDHITFVAVLTACSHIGLVEEGLNFLKSMESDYGIPPRMEHYACAV 551
Query: 481 DLYGRSGRLEEAKALIEEMPFKPDTTVWKTFLGACRSCGNIELACQVAGHLLEMEPEEHC 540
DL GR+ RL EA+ LIE MPFKPD VWKT LGACR CG+IELA QVA HLLE+EPEEHC
Sbjct: 552 DLLGRARRLGEARTLIESMPFKPDAMVWKTLLGACRVCGDIELATQVASHLLELEPEEHC 611
Query: 541 TYVLLSNMYGNLMRWDEKAKVKRLMKERGVKKVPGWSWIEVNNNVHAFIAQDHSHPSCQQ 600
TYVLLS++YG+L RWDEKA + RLM+ERGVKKVPGWSWIE+ N VHAF A+D SHP C++
Sbjct: 612 TYVLLSHLYGHLRRWDEKANLTRLMRERGVKKVPGWSWIEIKNQVHAFNAEDQSHPLCKE 671
Query: 601 IYFLLEVLLEEITRME 617
IY +L L+EEIT ++
Sbjct: 672 IYQMLGELMEEITWLD 687
BLAST of Cucsa.162960 vs. TAIR10
Match:
AT3G25970.1 (AT3G25970.1 Pentatricopeptide repeat (PPR) superfamily protein)
HSP 1 Score: 721.5 bits (1861), Expect = 4.5e-208
Identity = 347/614 (56.51%), Postives = 458/614 (74.59%), Query Frame = 1
Query: 1 MIAGHINCGNLEASWDVLRCMRSCGFELDRYTFGSMLKGIAFAGMFHLGQQVHSIIIKMG 60
MI+G+ +CG LE +W + CM+ G ++D Y+F +LKGIA F LG+QVH ++IK G
Sbjct: 72 MISGYTSCGKLEDAWCLFTCMKRSGSDVDGYSFSRLLKGIASVKRFDLGEQVHGLVIKGG 131
Query: 61 YAENVYAGSALLDMYAKCEKLEDAYLSFLSISKHNTVSWNAMINGYAQAGDRETAFWLLD 120
Y NVY GS+L+DMYAKCE++EDA+ +F IS+ N+VSWNA+I G+ Q D +TAFWLL
Sbjct: 132 YECNVYVGSSLVDMYAKCERVEDAFEAFKEISEPNSVSWNALIAGFVQVRDIKTAFWLLG 191
Query: 121 CMEQEGE-KVDDGTYAPLLPLLDDADFCNLTSQLHGKIIKHGLELVNTMCNALITSYSKC 180
ME + +D GT+APLL LLDD FCNL Q+H K++K GL+ T+CNA+I+SY+ C
Sbjct: 192 LMEMKAAVTMDAGTFAPLLTLLDDPMFCNLLKQVHAKVLKLGLQHEITICNAMISSYADC 251
Query: 181 GSLDDAKRIFDSSAGIRDLVTWNSLLAAYLLRSQEDLAFKLLIDMQEHGFEPDLYSYTSI 240
GS+ DAKR+FD G +DL++WNS++A + ++ AF+L I MQ H E D+Y+YT +
Sbjct: 252 GSVSDAKRVFDGLGGSKDLISWNSMIAGFSKHELKESAFELFIQMQRHWVETDIYTYTGL 311
Query: 241 ISACFNENISNNGRSLHGLVIKRGFEQSVPISNALISMYLKSDYGSMKEALCIFESLEFK 300
+SAC E G+SLHG+VIK+G EQ +NALISMY++ G+M++AL +FESL+ K
Sbjct: 312 LSACSGEEHQIFGKSLHGMVIKKGLEQVTSATNALISMYIQFPTGTMEDALSLFESLKSK 371
Query: 301 DRVSWNSILTGLSQTGSSEDAVKSFLHMRSAAMDIDHYSFSAVLRSCSDLATFQLGQQIH 360
D +SWNSI+TG +Q G SEDAVK F ++RS+ + +D Y+FSA+LRSCSDLAT QLGQQIH
Sbjct: 372 DLISWNSIITGFAQKGLSEDAVKFFSYLRSSEIKVDDYAFSALLRSCSDLATLQLGQQIH 431
Query: 361 VLALKYGLESNEFVSSSLIFMYSKCGIIEDARRSFEE-ASKNSSITWNALMFGYAQHGQC 420
LA K G SNEFV SSLI MYSKCGIIE AR+ F++ +SK+S++ WNA++ GYAQHG
Sbjct: 432 ALATKSGFVSNEFVISSLIVMYSKCGIIESARKCFQQISSKHSTVAWNAMILGYAQHGLG 491
Query: 421 NVALDLFFLMEEKKVKMDHITFVAVLTACSHIGLVEQGCKFLRCMESDYGVPPRMEHYAC 480
V+LDLF M + VK+DH+TF A+LTACSH GL+++G + L ME Y + PRMEHYA
Sbjct: 492 QVSLDLFSQMCNQNVKLDHVTFTAILTACSHTGLIQEGLELLNLMEPVYKIQPRMEHYAA 551
Query: 481 AVDLYGRSGRLEEAKALIEEMPFKPDTTVWKTFLGACRSCGNIELACQVAGHLLEMEPEE 540
AVDL GR+G + +AK LIE MP PD V KTFLG CR+CG IE+A QVA HLLE+EPE+
Sbjct: 552 AVDLLGRAGLVNKAKELIESMPLNPDPMVLKTFLGVCRACGEIEMATQVANHLLEIEPED 611
Query: 541 HCTYVLLSNMYGNLMRWDEKAKVKRLMKERGVKKVPGWSWIEVNNNVHAFIAQDHSHPSC 600
H TYV LS+MY +L +W+EKA VK++MKERGVKKVPGWSWIE+ N V AF A+D S+P C
Sbjct: 612 HFTYVSLSHMYSDLKKWEEKASVKKMMKERGVKKVPGWSWIEIRNQVKAFNAEDRSNPLC 671
Query: 601 QQIYFLLEVLLEEI 613
Q IY +++ L +E+
Sbjct: 672 QDIYMMIKDLTQEM 685
HSP 2 Score: 213.0 bits (541), Expect = 5.2e-55
Identity = 133/464 (28.66%), Postives = 236/464 (50.86%), Query Frame = 1
Query: 53 HSIIIKMGYAENVYAGSALLDMYAKCEKLEDAYLSFLSISKHNTVSWNAMINGYAQAGDR 112
H IK G ++Y + +LD Y K L A + F + K ++VSWN MI+GY G
Sbjct: 23 HCYAIKCGSISDIYVSNRILDSYIKFGFLGYANMLFDEMPKRDSVSWNTMISGYTSCGKL 82
Query: 113 ETAFWLLDCMEQEGEKVDDGTYAPLLPLLDDADFCNLTSQLHGKIIKHGLELVNTMCNAL 172
E A+ L CM++ G VD +++ LL + +L Q+HG +IK G E + ++L
Sbjct: 83 EDAWCLFTCMKRSGSDVDGYSFSRLLKGIASVKRFDLGEQVHGLVIKGGYECNVYVGSSL 142
Query: 173 ITSYSKCGSLDDAKRIFDSSAGIRDLVTWNSLLAAYLLRSQEDLAFKLLIDMQ-EHGFEP 232
+ Y+KC ++DA F + V+WN+L+A ++ AF LL M+ +
Sbjct: 143 VDMYAKCERVEDAFEAF-KEISEPNSVSWNALIAGFVQVRDIKTAFWLLGLMEMKAAVTM 202
Query: 233 DLYSYTSIISACFNENISNNGRSLHGLVIKRGFEQSVPISNALISMYLKSDYGSMKEALC 292
D ++ +++ + N + +H V+K G + + I NA+IS Y +D GS+ +A
Sbjct: 203 DAGTFAPLLTLLDDPMFCNLLKQVHAKVLKLGLQHEITICNAMISSY--ADCGSVSDAKR 262
Query: 293 IFESL-EFKDRVSWNSILTGLSQTGSSEDAVKSFLHMRSAAMDIDHYSFSAVLRSCSDLA 352
+F+ L KD +SWNS++ G S+ E A + F+ M+ ++ D Y+++ +L +CS
Sbjct: 263 VFDGLGGSKDLISWNSMIAGFSKHELKESAFELFIQMQRHWVETDIYTYTGLLSACSGEE 322
Query: 353 TFQLGQQIHVLALKYGLESNEFVSSSLIFMYSK--CGIIEDARRSFEEASKNSSITWNAL 412
G+ +H + +K GLE +++LI MY + G +EDA FE I+WN++
Sbjct: 323 HQIFGKSLHGMVIKKGLEQVTSATNALISMYIQFPTGTMEDALSLFESLKSKDLISWNSI 382
Query: 413 MFGYAQHGQCNVALDLFFLMEEKKVKMDHITFVAVLTACSHIGLVEQGCKFLRCMESDYG 472
+ G+AQ G A+ F + ++K+D F A+L +CS + ++ G + + + + G
Sbjct: 383 ITGFAQKGLSEDAVKFFSYLRSSEIKVDDYAFSALLRSCSDLATLQLG-QQIHALATKSG 442
Query: 473 VPPRMEHYACAVDLYGRSGRLEEAKALIEEMPFKPDTTVWKTFL 513
+ + +Y + G +E A+ +++ K T W +
Sbjct: 443 FVSNEFVISSLIVMYSKCGIIESARKCFQQISSKHSTVAWNAMI 482
HSP 3 Score: 62.8 bits (151), Expect = 8.7e-10
Identity = 48/189 (25.40%), Postives = 89/189 (47.09%), Query Frame = 1
Query: 347 SDLATFQLGQQIHVLALKYGLESNEFVSSSLIFMYSKCGIIEDARRSFEEASKNSSITWN 406
S L +FQ H A+K G S+ +VS+ ++ Y K G + A F+E K S++WN
Sbjct: 11 SSLNSFQKLSLTHCYAIKCGSISDIYVSNRILDSYIKFGFLGYANMLFDEMPKRDSVSWN 70
Query: 407 ALMFGYAQHGQCNVALDLFFLMEEKKVKMDHITFVAVLTACSHIGLVEQGCKFLRCMESD 466
++ GY G+ A LF M+ +D +F +L + + + G + + +
Sbjct: 71 TMISGYTSCGKLEDAWCLFTCMKRSGSDVDGYSFSRLLKGIASVKRFDLG-EQVHGLVIK 130
Query: 467 YGVPPRMEHYACAVDLYGRSGRLEEAKALIEEMPFKPDTTVWKTFLGACRSCGNIELACQ 526
G + + VD+Y + R+E+A +E+ +P++ W + +I+ A
Sbjct: 131 GGYECNVYVGSSLVDMYAKCERVEDAFEAFKEIS-EPNSVSWNALIAGFVQVRDIKTAFW 190
Query: 527 VAGHLLEME 536
+ G L+EM+
Sbjct: 191 LLG-LMEMK 196
BLAST of Cucsa.162960 vs. TAIR10
Match:
AT4G13650.1 (AT4G13650.1 Pentatricopeptide repeat (PPR) superfamily protein)
HSP 1 Score: 428.7 bits (1101), Expect = 6.1e-120
Identity = 222/602 (36.88%), Postives = 339/602 (56.31%), Query Frame = 1
Query: 1 MIAGHINCGNLEASWDVLRCMRSCGFELDRYTFGSMLKGIAFAGMFHLGQQVHSIIIKMG 60
+I G CG E + ++ + M G E D T S++ + G GQQ+H+ K+G
Sbjct: 360 LINGLSQCGYGEKAMELFKRMHLDGLEPDSNTLASLVVACSADGTLFRGQQLHAYTTKLG 419
Query: 61 YAENVYAGSALLDMYAKCEKLEDAYLSFLSISKHNTVSWNAMINGYAQAGDRETAFWLLD 120
+A N ALL++YAKC +E A FL N V WN M+ Y D +F +
Sbjct: 420 FASNNKIEGALLNLYAKCADIETALDYFLETEVENVVLWNVMLVAYGLLDDLRNSFRIFR 479
Query: 121 CMEQEGEKVDDGTYAPLLPLLDDADFCNLTSQLHGKIIKHGLELVNTMCNALITSYSKCG 180
M+ E + TY +L L Q+H +IIK +L +C+ LI Y+K G
Sbjct: 480 QMQIEEIVPNQYTYPSILKTCIRLGDLELGEQIHSQIIKTNFQLNAYVCSVLIDMYAKLG 539
Query: 181 SLDDAKRIFDSSAGIRDLVTWNSLLAAYLLRSQEDLAFKLLIDMQEHGFEPDLYSYTSII 240
LD A I AG +D+V+W +++A Y + +D A M + G D T+ +
Sbjct: 540 KLDTAWDILIRFAG-KDVVSWTTMIAGYTQYNFDDKALTTFRQMLDRGIRSDEVGLTNAV 599
Query: 241 SACFNENISNNGRSLHGLVIKRGFEQSVPISNALISMYLKSDYGSMKEALCIFESLEFKD 300
SAC G+ +H GF +P NAL+++Y S G ++E+ FE E D
Sbjct: 600 SACAGLQALKEGQQIHAQACVSGFSSDLPFQNALVTLY--SRCGKIEESYLAFEQTEAGD 659
Query: 301 RVSWNSILTGLSQTGSSEDAVKSFLHMRSAAMDIDHYSFSAVLRSCSDLATFQLGQQIHV 360
++WN++++G Q+G++E+A++ F+ M +D ++++F + +++ S+ A + G+Q+H
Sbjct: 660 NIAWNALVSGFQQSGNNEEALRVFVRMNREGIDNNNFTFGSAVKAASETANMKQGKQVHA 719
Query: 361 LALKYGLESNEFVSSSLIFMYSKCGIIEDARRSFEEASKNSSITWNALMFGYAQHGQCNV 420
+ K G +S V ++LI MY+KCG I DA + F E S + ++WNA++ Y++HG +
Sbjct: 720 VITKTGYDSETEVCNALISMYAKCGSISDAEKQFLEVSTKNEVSWNAIINAYSKHGFGSE 779
Query: 421 ALDLFFLMEEKKVKMDHITFVAVLTACSHIGLVEQGCKFLRCMESDYGVPPRMEHYACAV 480
ALD F M V+ +H+T V VL+ACSHIGLV++G + M S+YG+ P+ EHY C V
Sbjct: 780 ALDSFDQMIHSNVRPNHVTLVGVLSACSHIGLVDKGIAYFESMNSEYGLSPKPEHYVCVV 839
Query: 481 DLYGRSGRLEEAKALIEEMPFKPDTTVWKTFLGACRSCGNIELACQVAGHLLEMEPEEHC 540
D+ R+G L AK I+EMP KPD VW+T L AC N+E+ A HLLE+EPE+
Sbjct: 840 DMLTRAGLLSRAKEFIQEMPIKPDALVWRTLLSACVVHKNMEIGEFAAHHLLELEPEDSA 899
Query: 541 TYVLLSNMYGNLMRWDEKAKVKRLMKERGVKKVPGWSWIEVNNNVHAFIAQDHSHPSCQQ 600
TYVLLSN+Y +WD + ++ MKE+GVKK PG SWIEV N++H+F D +HP +
Sbjct: 900 TYVLLSNLYAVSKKWDARDLTRQKMKEKGVKKEPGQSWIEVKNSIHSFYVGDQNHPLADE 958
Query: 601 IY 603
I+
Sbjct: 960 IH 958
HSP 2 Score: 211.8 bits (538), Expect = 1.2e-54
Identity = 133/484 (27.48%), Postives = 229/484 (47.31%), Query Frame = 1
Query: 31 YTFGSMLKGIAFAGMFHLGQQVHSIIIKMGYAENVYAGSALLDMYAKCEKLEDAYLSFLS 90
Y F S+L +G+Q+H +++K+G++ + Y +AL+ +Y L A F +
Sbjct: 289 YAFSSVLSACKKIESLEIGEQLHGLVLKLGFSSDTYVCNALVSLYFHLGNLISAEHIFSN 348
Query: 91 ISKHNTVSWNAMINGYAQAGDRETAFWLLDCMEQEGEKVDDGTYAPLLPLLDDADFCNLT 150
+S+ + V++N +ING +Q G E A L M +G + D T A L+
Sbjct: 349 MSQRDAVTYNTLINGLSQCGYGEKAMELFKRMHLDGLEPDSNTLASLVVACSADGTLFRG 408
Query: 151 SQLHGKIIKHGLELVNTMCNALITSYSKCGSLDDAKRIFDSSAGIRDLVTWNSLLAAYLL 210
QLH K G N + AL+ Y+KC ++ A F + + ++V WN +L AY L
Sbjct: 409 QQLHAYTTKLGFASNNKIEGALLNLYAKCADIETALDYFLETE-VENVVLWNVMLVAYGL 468
Query: 211 RSQEDLAFKLLIDMQEHGFEPDLYSYTSIISACFNENISNNGRSLHGLVIKRGFEQSVPI 270
+F++ MQ P+ Y+Y SI+ C G +H +IK F+ + +
Sbjct: 469 LDDLRNSFRIFRQMQIEEIVPNQYTYPSILKTCIRLGDLELGEQIHSQIIKTNFQLNAYV 528
Query: 271 SNALISMYLKSDYGSMKEALCIFESLEFKDRVSWNSILTGLSQTGSSEDAVKSFLHMRSA 330
+ LI MY K G + A I KD VSW +++ G +Q + A+ +F M
Sbjct: 529 CSVLIDMYAK--LGKLDTAWDILIRFAGKDVVSWTTMIAGYTQYNFDDKALTTFRQMLDR 588
Query: 331 AMDIDHYSFSAVLRSCSDLATFQLGQQIHVLALKYGLESNEFVSSSLIFMYSKCGIIEDA 390
+ D + + +C+ L + GQQIH A G S+ ++L+ +YS+CG IE++
Sbjct: 589 GIRSDEVGLTNAVSACAGLQALKEGQQIHAQACVSGFSSDLPFQNALVTLYSRCGKIEES 648
Query: 391 RRSFEEASKNSSITWNALMFGYAQHGQCNVALDLFFLMEEKKVKMDHITFVAVLTACSHI 450
+FE+ +I WNAL+ G+ Q G AL +F M + + ++ TF + + A S
Sbjct: 649 YLAFEQTEAGDNIAWNALVSGFQQSGNNEEALRVFVRMNREGIDNNNFTFGSAVKAASET 708
Query: 451 GLVEQGCKFLRCMESDYGVPPRMEHYACAVDLYGRSGRLEEAKALIEEMPFKPDTTVWKT 510
++QG K + + + G E + +Y + G + +A+ E+ K + + W
Sbjct: 709 ANMKQG-KQVHAVITKTGYDSETEVCNALISMYAKCGSISDAEKQFLEVSTKNEVS-WNA 767
Query: 511 FLGA 515
+ A
Sbjct: 769 IINA 767
HSP 3 Score: 203.0 bits (515), Expect = 5.4e-52
Identity = 135/493 (27.38%), Postives = 239/493 (48.48%), Query Frame = 1
Query: 25 GFELDRYTFGSMLKG-IAFAGMFHLGQQVHSIIIKMGYAENVYAGSALLDMYAKCEKLED 84
G + T +L+G + G G+++HS I+K+G N L D Y L
Sbjct: 79 GIRPNHQTLKWLLEGCLKTNGSLDEGRKLHSQILKLGLDSNGCLSEKLFDFYLFKGDLYG 138
Query: 85 AYLSFLSISKHNTVSWNAMINGYAQAGDRETAFWLLDCMEQEGEKVDDGTYAPLLPLLDD 144
A+ F + + +WN MI A F L M E ++GT++ +L
Sbjct: 139 AFKVFDEMPERTIFTWNKMIKELASRNLIGEVFGLFVRMVSENVTPNEGTFSGVLEACRG 198
Query: 145 ADFC-NLTSQLHGKIIKHGLELVNTMCNALITSYSKCGSLDDAKRIFDSSAGIRDLVTWN 204
++ Q+H +I+ GL +CN LI YS+ G +D A+R+FD ++D +W
Sbjct: 199 GSVAFDVVEQIHARILYQGLRDSTVVCNPLIDLYSRNGFVDLARRVFDGLR-LKDHSSWV 258
Query: 205 SLLAAYLLRSQEDLAFKLLIDMQEHGFEPDLYSYTSIISACFNENISNNGRSLHGLVIKR 264
++++ E A +L DM G P Y+++S++SAC G LHGLV+K
Sbjct: 259 AMISGLSKNECEAEAIRLFCDMYVLGIMPTPYAFSSVLSACKKIESLEIGEQLHGLVLKL 318
Query: 265 GFEQSVPISNALISMYLKSDYGSMKEALCIFESLEFKDRVSWNSILTGLSQTGSSEDAVK 324
GF + NAL+S+Y G++ A IF ++ +D V++N+++ GLSQ G E A++
Sbjct: 319 GFSSDTYVCNALVSLYFH--LGNLISAEHIFSNMSQRDAVTYNTLINGLSQCGYGEKAME 378
Query: 325 SFLHMRSAAMDIDHYSFSAVLRSCSDLATFQLGQQIHVLALKYGLESNEFVSSSLIFMYS 384
F M ++ D + ++++ +CS T GQQ+H K G SN + +L+ +Y+
Sbjct: 379 LFKRMHLDGLEPDSNTLASLVVACSADGTLFRGQQLHAYTTKLGFASNNKIEGALLNLYA 438
Query: 385 KCGIIEDARRSFEEASKNSSITWNALMFGYAQHGQCNVALDLFFLMEEKKVKMDHITFVA 444
KC IE A F E + + WN ++ Y + +F M+ +++ + T+ +
Sbjct: 439 KCADIETALDYFLETEVENVVLWNVMLVAYGLLDDLRNSFRIFRQMQIEEIVPNQYTYPS 498
Query: 445 VLTACSHIGLVEQGCKF-LRCMESDYGVPPRMEHYACAV--DLYGRSGRLEEAKALIEEM 504
+L C +G +E G + + +++++ ++ Y C+V D+Y + G+L+ A ++
Sbjct: 499 ILKTCIRLGDLELGEQIHSQIIKTNF----QLNAYVCSVLIDMYAKLGKLDTAWDILIRF 558
Query: 505 PFKPDTTVWKTFL 513
K D W T +
Sbjct: 559 AGK-DVVSWTTMI 563
HSP 4 Score: 177.6 bits (449), Expect = 2.4e-44
Identity = 126/497 (25.35%), Postives = 229/497 (46.08%), Query Frame = 1
Query: 32 TFGSMLKGIAFAGM-FHLGQQVHSIIIKMGYAENVYAGSALLDMYAKCEKLEDAYLSFLS 91
TF +L+ + F + +Q+H+ I+ G ++ + L+D+Y++ ++ A F
Sbjct: 188 TFSGVLEACRGGSVAFDVVEQIHARILYQGLRDSTVVCNPLIDLYSRNGFVDLARRVFDG 247
Query: 92 ISKHNTVSWNAMINGYAQAGDRETAFWLLDCMEQEGEKVDDGTYAPLLPLLDDADFCNLT 151
+ + SW AMI+G ++ A L M G ++ +L + +
Sbjct: 248 LRLKDHSSWVAMISGLSKNECEAEAIRLFCDMYVLGIMPTPYAFSSVLSACKKIESLEIG 307
Query: 152 SQLHGKIIKHGLELVNTMCNALITSYSKCGSLDDAKRIFDSSAGIRDLVTWNSLLAAYLL 211
QLHG ++K G +CNAL++ Y G+L A+ IF S+ RD VT+N+L+
Sbjct: 308 EQLHGLVLKLGFSSDTYVCNALVSLYFHLGNLISAEHIF-SNMSQRDAVTYNTLINGLSQ 367
Query: 212 RSQEDLAFKLLIDMQEHGFEPDLYSYTSIISACFNENISNNGRSLHGLVIKRGFEQSVPI 271
+ A +L M G EPD + S++ AC + G+ LH K GF + I
Sbjct: 368 CGYGEKAMELFKRMHLDGLEPDSNTLASLVVACSADGTLFRGQQLHAYTTKLGFASNNKI 427
Query: 272 SNALISMYLKSDYGSMKEALCIFESLEFKDRVSWNSILTGLSQTGSSEDAVKSFLHMRSA 331
AL+++Y K ++ AL F E ++ V WN +L ++ + F M+
Sbjct: 428 EGALLNLYAKC--ADIETALDYFLETEVENVVLWNVMLVAYGLLDDLRNSFRIFRQMQIE 487
Query: 332 AMDIDHYSFSAVLRSCSDLATFQLGQQIHVLALKYGLESNEFVSSSLIFMYSKCGIIEDA 391
+ + Y++ ++L++C L +LG+QIH +K + N +V S LI MY+K G ++ A
Sbjct: 488 EIVPNQYTYPSILKTCIRLGDLELGEQIHSQIIKTNFQLNAYVCSVLIDMYAKLGKLDTA 547
Query: 392 RRSFEEASKNSSITWNALMFGYAQHGQCNVALDLFFLMEEKKVKMDHITFVAVLTACSHI 451
+ ++W ++ GY Q+ + AL F M ++ ++ D + ++AC+ +
Sbjct: 548 WDILIRFAGKDVVSWTTMIAGYTQYNFDDKALTTFRQMLDRGIRSDEVGLTNAVSACAGL 607
Query: 452 GLVEQGCKFLRCMESDYGVPPRMEHYACAVDLYGRSGRLEEAKALIEEMPFKPDTTVWKT 511
+++G + + G + V LY R G++EE+ E+ D W
Sbjct: 608 QALKEG-QQIHAQACVSGFSSDLPFQNALVTLYSRCGKIEESYLAFEQTE-AGDNIAWNA 667
Query: 512 FLGACRSCGNIELACQV 528
+ + GN E A +V
Sbjct: 668 LVSGFQQSGNNEEALRV 679
HSP 5 Score: 99.8 bits (247), Expect = 6.4e-21
Identity = 96/371 (25.88%), Postives = 165/371 (44.47%), Query Frame = 1
Query: 200 TWNSLLAAYLLRSQEDLAF--KLLIDMQEHGFEPDLYSYTSIISACFNENIS-NNGRSLH 259
T + AA + ED +F K + ++ G P+ + ++ C N S + GR LH
Sbjct: 49 TRRASFAAISVYISEDESFQEKRIDSVENRGIRPNHQTLKWLLEGCLKTNGSLDEGRKLH 108
Query: 260 GLVIKRGFEQSVPISNALISMYL-KSD-YGSMKEALCIFESLEFKDRVSWNSILTGLSQT 319
++K G + + +S L YL K D YG+ K +F+ + + +WN ++ L+
Sbjct: 109 SQILKLGLDSNGCLSEKLFDFYLFKGDLYGAFK----VFDEMPERTIFTWNKMIKELASR 168
Query: 320 GSSEDAVKSFLHMRSAAMDIDHYSFSAVLRSC-SDLATFQLGQQIHVLALKYGLESNEFV 379
+ F+ M S + + +FS VL +C F + +QIH L GL + V
Sbjct: 169 NLIGEVFGLFVRMVSENVTPNEGTFSGVLEACRGGSVAFDVVEQIHARILYQGLRDSTVV 228
Query: 380 SSSLIFMYSKCGIIEDARRSFEEASKNSSITWNALMFGYAQHGQCNVALDLFFLMEEKKV 439
+ LI +YS+ G ++ ARR F+ +W A++ G +++ A+ LF M +
Sbjct: 229 CNPLIDLYSRNGFVDLARRVFDGLRLKDHSSWVAMISGLSKNECEAEAIRLFCDMYVLGI 288
Query: 440 KMDHITFVAVLTACSHIGLVEQGCKFLRCMESDYGVPPRM----EHYAC--AVDLYGRSG 499
F +VL+AC I +E G E +G+ ++ + Y C V LY G
Sbjct: 289 MPTPYAFSSVLSACKKIESLEIG-------EQLHGLVLKLGFSSDTYVCNALVSLYFHLG 348
Query: 500 RLEEAKALIEEMPFKPDTTVWKTFLGACRSCGNIELACQV--AGHLLEMEPEEH--CTYV 555
L A+ + M + D + T + CG E A ++ HL +EP+ + + V
Sbjct: 349 NLISAEHIFSNMS-QRDAVTYNTLINGLSQCGYGEKAMELFKRMHLDGLEPDSNTLASLV 407
BLAST of Cucsa.162960 vs. TAIR10
Match:
AT2G27610.1 (AT2G27610.1 Tetratricopeptide repeat (TPR)-like superfamily protein)
HSP 1 Score: 404.1 bits (1037), Expect = 1.6e-112
Identity = 216/598 (36.12%), Postives = 352/598 (58.86%), Query Frame = 1
Query: 21 MRSCGFELDRYTFGSMLKGIAFAGMFHLGQQVHSIIIKMGYAENVYAGSALLDMYAKCEK 80
M++ G + + +TF + L +A G+ G QVH++++K G + + ++L+++Y KC
Sbjct: 185 MQNEGTQPNSFTFAAALGVLAEEGVGGRGLQVHTVVVKNGLDKTIPVSNSLINLYLKCGN 244
Query: 81 LEDAYLSFLSISKHNTVSWNAMINGYAQAGDRETAFWLLDCMEQEGEKVDDGTYAPLLPL 140
+ A + F + V+WN+MI+GYA G A + M ++ + ++A ++ L
Sbjct: 245 VRKARILFDKTEVKSVVTWNSMISGYAANGLDLEALGMFYSMRLNYVRLSESSFASVIKL 304
Query: 141 LDDADFCNLTSQLHGKIIKHGLELVNTMCNALITSYSKCGSLDDAKRIFDSSAGIRDLVT 200
+ T QLH ++K+G + AL+ +YSKC ++ DA R+F + ++V+
Sbjct: 305 CANLKELRFTEQLHCSVVKYGFLFDQNIRTALMVAYSKCTAMLDALRLFKEIGCVGNVVS 364
Query: 201 WNSLLAAYLLRSQEDLAFKLLIDMQEHGFEPDLYSYTSIISACFNENISNNGRSLHGLVI 260
W ++++ +L ++ A L +M+ G P+ ++Y+ I++A + S +H V+
Sbjct: 365 WTAMISGFLQNDGKEEAVDLFSEMKRKGVRPNEFTYSVILTALPVISPSE----VHAQVV 424
Query: 261 KRGFEQSVPISNALISMYLKSDYGSMKEALCIFESLEFKDRVSWNSILTGLSQTGSSEDA 320
K +E+S + AL+ Y+K G ++EA +F ++ KD V+W+++L G +QTG +E A
Sbjct: 425 KTNYERSSTVGTALLDAYVK--LGKVEEAAKVFSGIDDKDIVAWSAMLAGYAQTGETEAA 484
Query: 321 VKSFLHMRSAAMDIDHYSFSAVLRSCSDL-ATFQLGQQIHVLALKYGLESNEFVSSSLIF 380
+K F + + + ++FS++L C+ A+ G+Q H A+K L+S+ VSS+L+
Sbjct: 485 IKMFGELTKGGIKPNEFTFSSILNVCAATNASMGQGKQFHGFAIKSRLDSSLCVSSALLT 544
Query: 381 MYSKCGIIEDARRSFEEASKNSSITWNALMFGYAQHGQCNVALDLFFLMEEKKVKMDHIT 440
MY+K G IE A F+ + ++WN+++ GYAQHGQ ALD+F M+++KVKMD +T
Sbjct: 545 MYAKKGNIESAEEVFKRQREKDLVSWNSMISGYAQHGQAMKALDVFKEMKKRKVKMDGVT 604
Query: 441 FVAVLTACSHIGLVEQGCKFLRCMESDYGVPPRMEHYACAVDLYGRSGRLEEAKALIEEM 500
F+ V AC+H GLVE+G K+ M D + P EH +C VDLY R+G+LE+A +IE M
Sbjct: 605 FIGVFAACTHAGLVEEGEKYFDIMVRDCKIAPTKEHNSCMVDLYSRAGQLEKAMKVIENM 664
Query: 501 PFKPDTTVWKTFLGACRSCGNIELACQVAGHLLEMEPEEHCTYVLLSNMYGNLMRWDEKA 560
P +T+W+T L ACR EL A ++ M+PE+ YVLLSNMY W E+A
Sbjct: 665 PNPAGSTIWRTILAACRVHKKTELGRLAAEKIIAMKPEDSAAYVLLSNMYAESGDWQERA 724
Query: 561 KVKRLMKERGVKKVPGWSWIEVNNNVHAFIAQDHSHPSCQQIYFLLEVLLEEITRMED 618
KV++LM ER VKK PG+SWIEV N ++F+A D SHP QIY LE L TR++D
Sbjct: 725 KVRKLMNERNVKKEPGYSWIEVKNKTYSFLAGDRSHPLKDQIYMKLEDL---STRLKD 773
HSP 2 Score: 196.4 bits (498), Expect = 5.1e-50
Identity = 132/509 (25.93%), Postives = 242/509 (47.54%), Query Frame = 1
Query: 25 GFELDRYTFGSMLKGIAFAGMFHLGQQVHSIIIKMGYAENVYAGSALLDMYAKCEKLEDA 84
G E+D F S+LK A G+Q+H IK G+ ++V G++L+D Y K +D
Sbjct: 88 GMEMDCSIFSSVLKVSATLCDELFGRQLHCQCIKFGFLDDVSVGTSLVDTYMKGSNFKDG 147
Query: 85 YLSFLSISKHNTVSWNAMINGYAQAGDRETAFWLLDCMEQEGEKVDDGTYAPLLPLLDDA 144
F + + N V+W +I+GYA+ + L M+ EG + + T+A L +L +
Sbjct: 148 RKVFDEMKERNVVTWTTLISGYARNSMNDEVLTLFMRMQNEGTQPNSFTFAAALGVLAEE 207
Query: 145 DFCNLTSQLHGKIIKHGLELVNTMCNALITSYSKCGSLDDAKRIFDSSAGIRDLVTWNSL 204
Q+H ++K+GL+ + N+LI Y KCG++ A+ +FD + ++ +VTWNS+
Sbjct: 208 GVGGRGLQVHTVVVKNGLDKTIPVSNSLINLYLKCGNVRKARILFDKTE-VKSVVTWNSM 267
Query: 205 LAAYLLRSQEDLAFKLLIDMQEHGFEPDLYSYTSIISACFNENISNNGRSLHGLVIKRGF 264
++ Y + A + M+ + S+ S+I C N LH V+K GF
Sbjct: 268 ISGYAANGLDLEALGMFYSMRLNYVRLSESSFASVIKLCANLKELRFTEQLHCSVVKYGF 327
Query: 265 EQSVPISNALISMYLKSDYGSMKEALCIFESLE-FKDRVSWNSILTGLSQTGSSEDAVKS 324
I AL+ Y K +M +AL +F+ + + VSW ++++G Q E+AV
Sbjct: 328 LFDQNIRTALMVAYSKCT--AMLDALRLFKEIGCVGNVVSWTAMISGFLQNDGKEEAVDL 387
Query: 325 FLHMRSAAMDIDHYSFSAVLRSCSDLATFQLGQQIHVLALKYGLESNEFVSSSLIFMYSK 384
F M+ + + +++S +L + ++ ++H +K E + V ++L+ Y K
Sbjct: 388 FSEMKRKGVRPNEFTYSVILTALPVISP----SEVHAQVVKTNYERSSTVGTALLDAYVK 447
Query: 385 CGIIEDARRSFEEASKNSSITWNALMFGYAQHGQCNVALDLFFLMEEKKVKMDHITFVAV 444
G +E+A + F + W+A++ GYAQ G+ A+ +F + + +K + TF ++
Sbjct: 448 LGKVEEAAKVFSGIDDKDIVAWSAMLAGYAQTGETEAAIKMFGELTKGGIKPNEFTFSSI 507
Query: 445 LTACSHIGL-VEQGCKFLRCMESDYGVPPRMEHYACA----VDLYGRSGRLEEAKALIEE 504
L C+ + QG +F + + R++ C + +Y + G +E A+ + +
Sbjct: 508 LNVCAATNASMGQGKQF-----HGFAIKSRLDSSLCVSSALLTMYAKKGNIESAEEVFKR 567
Query: 505 MPFKPDTTVWKTFLGACRSCGNIELACQV 528
K D W + + G A V
Sbjct: 568 QREK-DLVSWNSMISGYAQHGQAMKALDV 583
HSP 3 Score: 189.5 bits (480), Expect = 6.2e-48
Identity = 122/464 (26.29%), Postives = 241/464 (51.94%), Query Frame = 1
Query: 80 KLEDAYLSFLSISKHNTVSWNAMINGYAQAGDRETAFWLLDCMEQEGEKVDDGTYAPLLP 139
+L +A+ F + S+ +++ G+++ G + A L + + G ++D ++ +L
Sbjct: 42 RLYNAHNLFDKSPGRDRESYISLLFGFSRDGRTQEAKRLFLNIHRLGMEMDCSIFSSVLK 101
Query: 140 LLDDADFCN--LTSQLHGKIIKHGLELVNTMCNALITSYSKCGSLDDAKRIFDSSAGIRD 199
+ A C+ QLH + IK G ++ +L+ +Y K + D +++FD R+
Sbjct: 102 V--SATLCDELFGRQLHCQCIKFGFLDDVSVGTSLVDTYMKGSNFKDGRKVFDEMKE-RN 161
Query: 200 LVTWNSLLAAYLLRSQEDLAFKLLIDMQEHGFEPDLYSYTSIISACFNENISNNGRSLHG 259
+VTW +L++ Y S D L + MQ G +P+ +++ + + E + G +H
Sbjct: 162 VVTWTTLISGYARNSMNDEVLTLFMRMQNEGTQPNSFTFAAALGVLAEEGVGGRGLQVHT 221
Query: 260 LVIKRGFEQSVPISNALISMYLKSDYGSMKEALCIFESLEFKDRVSWNSILTGLSQTGSS 319
+V+K G ++++P+SN+LI++YLK G++++A +F+ E K V+WNS+++G + G
Sbjct: 222 VVVKNGLDKTIPVSNSLINLYLKC--GNVRKARILFDKTEVKSVVTWNSMISGYAANGLD 281
Query: 320 EDAVKSFLHMRSAAMDIDHYSFSAVLRSCSDLATFQLGQQIHVLALKYGLESNEFVSSSL 379
+A+ F MR + + SF++V++ C++L + +Q+H +KYG ++ + ++L
Sbjct: 282 LEALGMFYSMRLNYVRLSESSFASVIKLCANLKELRFTEQLHCSVVKYGFLFDQNIRTAL 341
Query: 380 IFMYSKCGIIEDARRSFEE-ASKNSSITWNALMFGYAQHGQCNVALDLFFLMEEKKVKMD 439
+ YSKC + DA R F+E + ++W A++ G+ Q+ A+DLF M+ K V+ +
Sbjct: 342 MVAYSKCTAMLDALRLFKEIGCVGNVVSWTAMISGFLQNDGKEEAVDLFSEMKRKGVRPN 401
Query: 440 HITFVAVLTACSHIGLVEQGCKFLRCMESDYGVPPRMEHYACAVDLYGRSGRLEEAKALI 499
T+ +LTA I E + ++ ++Y + +D Y + G++EEA +
Sbjct: 402 EFTYSVILTALPVISPSEVHAQVVK---TNYERSSTVG--TALLDAYVKLGKVEEAAKVF 461
Query: 500 EEMPFKPDTTVWKTFLGACRSCGNIELACQVAGHLLE--MEPEE 539
+ K D W L G E A ++ G L + ++P E
Sbjct: 462 SGIDDK-DIVAWSAMLAGYAQTGETEAAIKMFGELTKGGIKPNE 494
BLAST of Cucsa.162960 vs. TAIR10
Match:
AT4G21300.1 (AT4G21300.1 Tetratricopeptide repeat (TPR)-like superfamily protein)
HSP 1 Score: 390.6 bits (1002), Expect = 1.8e-108
Identity = 220/616 (35.71%), Postives = 340/616 (55.19%), Query Frame = 1
Query: 1 MIAGHINCGNLEASWDVLRCMRSCGFELDRYTFGSMLKGIAFAGMFHLGQQVHSIIIKMG 60
M+ G+ CG L++ MR + TF +L A + LG Q+H +++ G
Sbjct: 210 MLNGYAKCGALDSVIKGFSVMRMDQISPNAVTFDCVLSVCASKLLIDLGVQLHGLVVVSG 269
Query: 61 YAENVYAGSALLDMYAKCEKLEDAYLSFLSISKHNTVSWNAMINGYAQAGDRETAFWLLD 120
++LL MY+KC + +DA F +S+ +TV+WN MI+GY Q+G E +
Sbjct: 270 VDFEGSIKNSLLSMYSKCGRFDDASKLFRMMSRADTVTWNCMISGYVQSGLMEESLTFFY 329
Query: 121 CMEQEGEKVDDGTYAPLLPLLDDADFCNLTSQLHGKIIKHGLELVNTMCNALITSYSKCG 180
M G D T++ LLP + + Q+H I++H + L + +ALI +Y KC
Sbjct: 330 EMISSGVLPDAITFSSLLPSVSKFENLEYCKQIHCYIMRHSISLDIFLTSALIDAYFKCR 389
Query: 181 SLDDAKRIFDSSAGIRDLVTWNSLLAAYL---LRSQEDLAFKLLIDMQEHGFEPDLYSYT 240
+ A+ IF + D+V + ++++ YL L F+ L+ ++ E L S
Sbjct: 390 GVSMAQNIFSQCNSV-DVVVFTAMISGYLHNGLYIDSLEMFRWLVKVKISPNEITLVSIL 449
Query: 241 SIISACFNENISNNGRSLHGLVIKRGFEQSVPISNALISMYLKSDYGSMKEALCIFESLE 300
+I + GR LHG +IK+GF+ I A+I MY K G M A IFE L
Sbjct: 450 PVIGILLALKL---GRELHGFIIKKGFDNRCNIGCAVIDMYAKC--GRMNLAYEIFERLS 509
Query: 301 FKDRVSWNSILTGLSQTGSSEDAVKSFLHMRSAAMDIDHYSFSAVLRSCSDLATFQLGQQ 360
+D VSWNS++T +Q+ + A+ F M + + D S SA L +C++L + G+
Sbjct: 510 KRDIVSWNSMITRCAQSDNPSAAIDIFRQMGVSGICYDCVSISAALSACANLPSESFGKA 569
Query: 361 IHVLALKYGLESNEFVSSSLIFMYSKCGIIEDARRSFEEASKNSSITWNALMFGYAQHGQ 420
IH +K+ L S+ + S+LI MY+KCG ++ A F+ + + ++WN+++ HG+
Sbjct: 570 IHGFMIKHSLASDVYSESTLIDMYAKCGNLKAAMNVFKTMKEKNIVSWNSIIAACGNHGK 629
Query: 421 CNVALDLFFLMEEKK-VKMDHITFVAVLTACSHIGLVEQGCKFLRCMESDYGVPPRMEHY 480
+L LF M EK ++ D ITF+ ++++C H+G V++G +F R M DYG+ P+ EHY
Sbjct: 630 LKDSLCLFHEMVEKSGIRPDQITFLEIISSCCHVGDVDEGVRFFRSMTEDYGIQPQQEHY 689
Query: 481 ACAVDLYGRSGRLEEAKALIEEMPFKPDTTVWKTFLGACRSCGNIELACQVAGHLLEMEP 540
AC VDL+GR+GRL EA ++ MPF PD VW T LGACR N+ELA + L++++P
Sbjct: 690 ACVVDLFGRAGRLTEAYETVKSMPFPPDAGVWGTLLGACRLHKNVELAEVASSKLMDLDP 749
Query: 541 EEHCTYVLLSNMYGNLMRWDEKAKVKRLMKERGVKKVPGWSWIEVNNNVHAFIAQDHSHP 600
YVL+SN + N W+ KV+ LMKER V+K+PG+SWIE+N H F++ D +HP
Sbjct: 750 SNSGYYVLISNAHANAREWESVTKVRSLMKEREVQKIPGYSWIEINKRTHLFVSGDVNHP 809
Query: 601 SCQQIYFLLEVLLEEI 613
IY LL LL E+
Sbjct: 810 ESSHIYSLLNSLLGEL 819
HSP 2 Score: 167.5 bits (423), Expect = 2.5e-41
Identity = 117/502 (23.31%), Postives = 233/502 (46.41%), Query Frame = 1
Query: 25 GFELDRYTFGSMLKGIA----FAGMFHLGQQVHSIIIKMGYAENVYAGSALLDMYAKCEK 84
G D TF ++K F G+ L V S+ G N + S+L+ Y + K
Sbjct: 133 GVSPDVSTFPCLVKACVALKNFKGIDFLSDTVSSL----GMDCNEFVASSLIKAYLEYGK 192
Query: 85 LEDAYLSFLSISKHNTVSWNAMINGYAQAGDRETAFWLLDCMEQEGEKVDDGTYAPLLPL 144
++ F + + + V WN M+NGYA+ G ++ M + + T+ +L +
Sbjct: 193 IDVPSKLFDRVLQKDCVIWNVMLNGYAKCGALDSVIKGFSVMRMDQISPNAVTFDCVLSV 252
Query: 145 LDDADFCNLTSQLHGKIIKHGLELVNTMCNALITSYSKCGSLDDAKRIFDSSAGIRDLVT 204
+L QLHG ++ G++ ++ N+L++ YSKCG DDA ++F + D VT
Sbjct: 253 CASKLLIDLGVQLHGLVVVSGVDFEGSIKNSLLSMYSKCGRFDDASKLFRMMSRA-DTVT 312
Query: 205 WNSLLAAYLLRSQEDLAFKLLIDMQEHGFEPDLYSYTSIISACFNENISNNGRSLHGLVI 264
WN +++ Y+ + + +M G PD +++S++ + + +H ++
Sbjct: 313 WNCMISGYVQSGLMEESLTFFYEMISSGVLPDAITFSSLLPSVSKFENLEYCKQIHCYIM 372
Query: 265 KRGFEQSVPISNALISMYLKSDYGSMKEALCIFESLEFKDRVSWNSILTGLSQTGSSEDA 324
+ + +++ALI Y K SM + IF D V + ++++G G D+
Sbjct: 373 RHSISLDIFLTSALIDAYFKCRGVSMAQN--IFSQCNSVDVVVFTAMISGYLHNGLYIDS 432
Query: 325 VKSFLHMRSAAMDIDHYSFSAVLRSCSDLATFQLGQQIHVLALKYGLESNEFVSSSLIFM 384
++ F + + + + ++L L +LG+++H +K G ++ + ++I M
Sbjct: 433 LEMFRWLVKVKISPNEITLVSILPVIGILLALKLGRELHGFIIKKGFDNRCNIGCAVIDM 492
Query: 385 YSKCGIIEDARRSFEEASKNSSITWNALMFGYAQHGQCNVALDLFFLMEEKKVKMDHITF 444
Y+KCG + A FE SK ++WN+++ AQ + A+D+F M + D ++
Sbjct: 493 YAKCGRMNLAYEIFERLSKRDIVSWNSMITRCAQSDNPSAAIDIFRQMGVSGICYDCVSI 552
Query: 445 VAVLTACSHIGLVEQGCKFLRCMESDYGVPPRMEHYACAVDLYGRSGRLEEAKALIEEMP 504
A L+AC+++ G K + + + + + +D+Y + G L+ A + + M
Sbjct: 553 SAALSACANLPSESFG-KAIHGFMIKHSLASDVYSESTLIDMYAKCGNLKAAMNVFKTMK 612
Query: 505 FKPDTTVWKTFLGACRSCGNIE 523
K + W + + AC + G ++
Sbjct: 613 EK-NIVSWNSIIAACGNHGKLK 625
HSP 3 Score: 164.9 bits (416), Expect = 1.6e-40
Identity = 119/494 (24.09%), Postives = 223/494 (45.14%), Query Frame = 1
Query: 36 MLKGIAFAGMFHLGQQVHSIIIKMGYAENVYAGSALLDMYAKCEKLEDAYLSF--LSISK 95
+L+ + + G+QVH+ +I + + Y +L MYA C D F L + +
Sbjct: 41 LLQACSNPNLLRQGKQVHAFLIVNSISGDSYTDERILGMYAMCGSFSDCGKMFYRLDLRR 100
Query: 96 HNTVSWNAMINGYAQAGDRETAFWLLDCMEQEGEKVDDGTYAPLLPLLDDADFCNLTSQL 155
+ WN++I+ + + G A M G D T+ L+ L
Sbjct: 101 SSIRPWNSIISSFVRNGLLNQALAFYFKMLCFGVSPDVSTFPCLVKACVALKNFKGIDFL 160
Query: 156 HGKIIKHGLELVNTMCNALITSYSKCGSLDDAKRIFDSSAGIRDLVTWNSLLAAYLLRSQ 215
+ G++ + ++LI +Y + G +D ++FD +D V WN +L Y
Sbjct: 161 SDTVSSLGMDCNEFVASSLIKAYLEYGKIDVPSKLFDRVLQ-KDCVIWNVMLNGYAKCGA 220
Query: 216 EDLAFKLLIDMQEHGFEPDLYSYTSIISACFNENISNNGRSLHGLVIKRGFEQSVPISNA 275
D K M+ P+ ++ ++S C ++ + + G LHGLV+ G + I N+
Sbjct: 221 LDSVIKGFSVMRMDQISPNAVTFDCVLSVCASKLLIDLGVQLHGLVVVSGVDFEGSIKNS 280
Query: 276 LISMYLKSDYGSMKEALCIFESLEFKDRVSWNSILTGLSQTGSSEDAVKSFLHMRSAAMD 335
L+SMY K G +A +F + D V+WN +++G Q+G E+++ F M S+ +
Sbjct: 281 LLSMYSKC--GRFDDASKLFRMMSRADTVTWNCMISGYVQSGLMEESLTFFYEMISSGVL 340
Query: 336 IDHYSFSAVLRSCSDLATFQLGQQIHVLALKYGLESNEFVSSSLIFMYSKCGIIEDARRS 395
D +FS++L S S + +QIH +++ + + F++S+LI Y KC + A+
Sbjct: 341 PDAITFSSLLPSVSKFENLEYCKQIHCYIMRHSISLDIFLTSALIDAYFKCRGVSMAQNI 400
Query: 396 FEEASKNSSITWNALMFGYAQHGQCNVALDLFFLMEEKKVKMDHITFVAVLTACSHIGLV 455
F + + + + A++ GY +G +L++F + + K+ + IT V++L + +
Sbjct: 401 FSQCNSVDVVVFTAMISGYLHNGLYIDSLEMFRWLVKVKISPNEITLVSILPVIGILLAL 460
Query: 456 EQGCKFLRCMESDYGVPPRMEHYACAVDLYGRSGRLEEAKALIEEMPFKPDTTVWKTFLG 515
+ G + L G R +D+Y + GR+ A + E + K D W + +
Sbjct: 461 KLG-RELHGFIIKKGFDNRCNIGCAVIDMYAKCGRMNLAYEIFERLS-KRDIVSWNSMIT 520
Query: 516 ACRSCGNIELACQV 528
C N A +
Sbjct: 521 RCAQSDNPSAAIDI 529
BLAST of Cucsa.162960 vs. TAIR10
Match:
AT2G13600.1 (AT2G13600.1 Pentatricopeptide repeat (PPR) superfamily protein)
HSP 1 Score: 379.4 bits (973), Expect = 4.2e-105
Identity = 218/629 (34.66%), Postives = 342/629 (54.37%), Query Frame = 1
Query: 25 GFELDRYTFGSMLKGIAFAGMFHLGQQVHSIIIKMGYAENVYAGSALLDMYAKCEKLEDA 84
GF + + ++ + G G+QV + + N+Y ++++ K L++A
Sbjct: 50 GFSNEIFIQNRLIDAYSKCGSLEDGRQVFDKMPQ----RNIYTWNSVVTGLTKLGFLDEA 109
Query: 85 YLSFLSISKHNTVSWNAMINGYAQAGDRETAFWLLDCMEQEGEKVDDGTYAPLLPLLDDA 144
F S+ + + +WN+M++G+AQ E A M +EG +++ ++A +L
Sbjct: 110 DSLFRSMPERDQCTWNSMVSGFAQHDRCEEALCYFAMMHKEGFVLNEYSFASVLSACSGL 169
Query: 145 DFCNLTSQLHGKIIKHGLELVNTMCNALITSYSKCGSLDDAKRIFDSSAGIRDLVTWNSL 204
+ N Q+H I K + +AL+ YSKCG+++DA+R+FD G R++V+WNSL
Sbjct: 170 NDMNKGVQVHSLIAKSPFLSDVYIGSALVDMYSKCGNVNDAQRVFDEM-GDRNVVSWNSL 229
Query: 205 LAAYLLRSQEDLAFKLLIDMQEHGFEPDLYSYTSIISACFNENISNNGRSLHGLVIK--- 264
+ + A + M E EPD + S+ISAC + + G+ +HG V+K
Sbjct: 230 ITCFEQNGPAVEALDVFQMMLESRVEPDEVTLASVISACASLSAIKVGQEVHGRVVKNDK 289
Query: 265 ------------------------RGFEQSVPISNALISMYLKSDYG---SMKEALCIFE 324
R S+PI N + + S Y S K A +F
Sbjct: 290 LRNDIILSNAFVDMYAKCSRIKEARFIFDSMPIRNVIAETSMISGYAMAASTKAARLMFT 349
Query: 325 SLEFKDRVSWNSILTGLSQTGSSEDAVKSFLHMRSAAMDIDHYSFSAVLRSCSDLATFQL 384
+ ++ VSWN+++ G +Q G +E+A+ F ++ ++ HYSF+ +L++C+DLA L
Sbjct: 350 KMAERNVVSWNALIAGYTQNGENEEALSLFCLLKRESVCPTHYSFANILKACADLAELHL 409
Query: 385 GQQIHVLALKYGL------ESNEFVSSSLIFMYSKCGIIEDARRSFEEASKNSSITWNAL 444
G Q HV LK+G E + FV +SLI MY KCG +E+ F + + ++WNA+
Sbjct: 410 GMQAHVHVLKHGFKFQSGEEDDIFVGNSLIDMYVKCGCVEEGYLVFRKMMERDCVSWNAM 469
Query: 445 MFGYAQHGQCNVALDLFFLMEEKKVKMDHITFVAVLTACSHIGLVEQGCKFLRCMESDYG 504
+ G+AQ+G N AL+LF M E K DHIT + VL+AC H G VE+G + M D+G
Sbjct: 470 IIGFAQNGYGNEALELFREMLESGEKPDHITMIGVLSACGHAGFVEEGRHYFSSMTRDFG 529
Query: 505 VPPRMEHYACAVDLYGRSGRLEEAKALIEEMPFKPDTTVWKTFLGACRSCGNIELACQVA 564
V P +HY C VDL GR+G LEEAK++IEEMP +PD+ +W + L AC+ NI L VA
Sbjct: 530 VAPLRDHYTCMVDLLGRAGFLEEAKSMIEEMPMQPDSVIWGSLLAACKVHRNITLGKYVA 589
Query: 565 GHLLEMEPEEHCTYVLLSNMYGNLMRWDEKAKVKRLMKERGVKKVPGWSWIEVNNNVHAF 618
LLE+EP YVLLSNMY L +W++ V++ M++ GV K PG SWI++ + H F
Sbjct: 590 EKLLEVEPSNSGPYVLLSNMYAELGKWEDVMNVRKSMRKEGVTKQPGCSWIKIQGHDHVF 649
HSP 2 Score: 96.3 bits (238), Expect = 7.1e-20
Identity = 63/214 (29.44%), Postives = 101/214 (47.20%), Query Frame = 1
Query: 1 MIAGHINCGNLEASWDVLRCMRSCGFELDRYTFGSMLKGIAFAGMFHLGQQVHSIIIKMG 60
+IAG+ G E + + ++ Y+F ++LK A HLG Q H ++K G
Sbjct: 357 LIAGYTQNGENEEALSLFCLLKRESVCPTHYSFANILKACADLAELHLGMQAHVHVLKHG 416
Query: 61 YA------ENVYAGSALLDMYAKCEKLEDAYLSFLSISKHNTVSWNAMINGYAQAGDRET 120
+ ++++ G++L+DMY KC +E+ YL F + + + VSWNAMI G+AQ G
Sbjct: 417 FKFQSGEEDDIFVGNSLIDMYVKCGCVEEGYLVFRKMMERDCVSWNAMIIGFAQNGYGNE 476
Query: 121 AFWLLDCMEQEGEKVDDGTYAPLLPLLDDADFCNLTSQLHGKIIK-HGLELVNTMCNALI 180
A L M + GEK D T +L A F + + G+ + ++
Sbjct: 477 ALELFREMLESGEKPDHITMIGVLSACGHAGFVEEGRHYFSSMTRDFGVAPLRDHYTCMV 536
Query: 181 TSYSKCGSLDDAKRIFDSSAGIRDLVTWNSLLAA 208
+ G L++AK + + D V W SLLAA
Sbjct: 537 DLLGRAGFLEEAKSMIEEMPMQPDSVIWGSLLAA 570
HSP 3 Score: 69.3 bits (168), Expect = 9.3e-12
Identity = 57/216 (26.39%), Postives = 100/216 (46.30%), Query Frame = 1
Query: 320 AVKSFLHMRSAAMDI-DHYSFSAVLRSC--SDLATFQLGQQIHVLALKYGLESNEFVSSS 379
A KSFL + + D F+ +L SC S L+ + + +H +K G + F+ +
Sbjct: 2 ATKSFLKLAADLSSFTDSSPFAKLLDSCIKSKLSAIYV-RYVHASVIKSGFSNEIFIQNR 61
Query: 380 LIFMYSKCGIIEDARRSFEEASKNSSITWNALMFGYAQHGQCNVALDLFFLMEEKKVKMD 439
LI YSKCG +ED R+ F++ + + TWN+++ G + G + A LF M E+ D
Sbjct: 62 LIDAYSKCGSLEDGRQVFDKMPQRNIYTWNSVVTGLTKLGFLDEADSLFRSMPER----D 121
Query: 440 HITFVAVLTACSHIGLVEQGCKFLRCMESDYGVPPRMEHYACAVDLYGRSGRLE-----E 499
T+ ++++ + E+ + M + V + Y+ A L SG + +
Sbjct: 122 QCTWNSMVSGFAQHDRCEEALCYFAMMHKEGFV---LNEYSFASVLSACSGLNDMNKGVQ 181
Query: 500 AKALIEEMPFKPDTTVWKTFLGACRSCGNIELACQV 528
+LI + PF D + + CGN+ A +V
Sbjct: 182 VHSLIAKSPFLSDVYIGSALVDMYSKCGNVNDAQRV 209
HSP 4 Score: 54.7 bits (130), Expect = 2.4e-07
Identity = 63/314 (20.06%), Postives = 128/314 (40.76%), Query Frame = 1
Query: 232 DLYSYTSIISACFNENISN-NGRSLHGLVIKRGFEQSVPISNALISMYLKSDYGSMKEAL 291
D + ++ +C +S R +H VIK GF + I N LI Y K GS+++
Sbjct: 18 DSSPFAKLLDSCIKSKLSAIYVRYVHASVIKSGFSNEIFIQNRLIDAYSKC--GSLEDGR 77
Query: 292 CIFESLEFKDRVSWNSILTGLSQTGSSEDAVKSFLHMRSAAMDIDHYSFSAVLRSCSDLA 351
+F+ + ++ +WNS++TGL++ G ++A F M + D ++++++ +
Sbjct: 78 QVFDKMPQRNIYTWNSVVTGLTKLGFLDEADSLFRSM----PERDQCTWNSMVSGFAQHD 137
Query: 352 TFQLGQQIHVLALKYGLESNEFVSSSLIFMYSKCGIIEDARRSFEEASKNSSITW----- 411
+ + K G NE+ +S++ S C + D + + S + +
Sbjct: 138 RCEEALCYFAMMHKEGFVLNEYSFASVL---SACSGLNDMNKGVQVHSLIAKSPFLSDVY 197
Query: 412 --NALMFGYAQHGQCNVALDLFFLMEEKKVKMDHITFVAVLTACSHIGLVEQGCKFLRCM 471
+AL+ Y++ G N A +F M ++ V +++ +++T G + + M
Sbjct: 198 IGSALVDMYSKCGNVNDAQRVFDEMGDRNV----VSWNSLITCFEQNGPAVEALDVFQMM 257
Query: 472 ESDYGVPPRME----HYACA-------------------------------VDLYGRSGR 503
P + ACA VD+Y + R
Sbjct: 258 LESRVEPDEVTLASVISACASLSAIKVGQEVHGRVVKNDKLRNDIILSNAFVDMYAKCSR 317
BLAST of Cucsa.162960 vs. NCBI nr
Match:
gi|449442142|ref|XP_004138841.1| (PREDICTED: putative pentatricopeptide repeat-containing protein At3g25970 [Cucumis sativus])
HSP 1 Score: 1260.7 bits (3261), Expect = 0.0e+00
Identity = 619/619 (100.00%), Postives = 619/619 (100.00%), Query Frame = 1
Query: 1 MIAGHINCGNLEASWDVLRCMRSCGFELDRYTFGSMLKGIAFAGMFHLGQQVHSIIIKMG 60
MIAGHINCGNLEASWDVLRCMRSCGFELDRYTFGSMLKGIAFAGMFHLGQQVHSIIIKMG
Sbjct: 70 MIAGHINCGNLEASWDVLRCMRSCGFELDRYTFGSMLKGIAFAGMFHLGQQVHSIIIKMG 129
Query: 61 YAENVYAGSALLDMYAKCEKLEDAYLSFLSISKHNTVSWNAMINGYAQAGDRETAFWLLD 120
YAENVYAGSALLDMYAKCEKLEDAYLSFLSISKHNTVSWNAMINGYAQAGDRETAFWLLD
Sbjct: 130 YAENVYAGSALLDMYAKCEKLEDAYLSFLSISKHNTVSWNAMINGYAQAGDRETAFWLLD 189
Query: 121 CMEQEGEKVDDGTYAPLLPLLDDADFCNLTSQLHGKIIKHGLELVNTMCNALITSYSKCG 180
CMEQEGEKVDDGTYAPLLPLLDDADFCNLTSQLHGKIIKHGLELVNTMCNALITSYSKCG
Sbjct: 190 CMEQEGEKVDDGTYAPLLPLLDDADFCNLTSQLHGKIIKHGLELVNTMCNALITSYSKCG 249
Query: 181 SLDDAKRIFDSSAGIRDLVTWNSLLAAYLLRSQEDLAFKLLIDMQEHGFEPDLYSYTSII 240
SLDDAKRIFDSSAGIRDLVTWNSLLAAYLLRSQEDLAFKLLIDMQEHGFEPDLYSYTSII
Sbjct: 250 SLDDAKRIFDSSAGIRDLVTWNSLLAAYLLRSQEDLAFKLLIDMQEHGFEPDLYSYTSII 309
Query: 241 SACFNENISNNGRSLHGLVIKRGFEQSVPISNALISMYLKSDYGSMKEALCIFESLEFKD 300
SACFNENISNNGRSLHGLVIKRGFEQSVPISNALISMYLKSDYGSMKEALCIFESLEFKD
Sbjct: 310 SACFNENISNNGRSLHGLVIKRGFEQSVPISNALISMYLKSDYGSMKEALCIFESLEFKD 369
Query: 301 RVSWNSILTGLSQTGSSEDAVKSFLHMRSAAMDIDHYSFSAVLRSCSDLATFQLGQQIHV 360
RVSWNSILTGLSQTGSSEDAVKSFLHMRSAAMDIDHYSFSAVLRSCSDLATFQLGQQIHV
Sbjct: 370 RVSWNSILTGLSQTGSSEDAVKSFLHMRSAAMDIDHYSFSAVLRSCSDLATFQLGQQIHV 429
Query: 361 LALKYGLESNEFVSSSLIFMYSKCGIIEDARRSFEEASKNSSITWNALMFGYAQHGQCNV 420
LALKYGLESNEFVSSSLIFMYSKCGIIEDARRSFEEASKNSSITWNALMFGYAQHGQCNV
Sbjct: 430 LALKYGLESNEFVSSSLIFMYSKCGIIEDARRSFEEASKNSSITWNALMFGYAQHGQCNV 489
Query: 421 ALDLFFLMEEKKVKMDHITFVAVLTACSHIGLVEQGCKFLRCMESDYGVPPRMEHYACAV 480
ALDLFFLMEEKKVKMDHITFVAVLTACSHIGLVEQGCKFLRCMESDYGVPPRMEHYACAV
Sbjct: 490 ALDLFFLMEEKKVKMDHITFVAVLTACSHIGLVEQGCKFLRCMESDYGVPPRMEHYACAV 549
Query: 481 DLYGRSGRLEEAKALIEEMPFKPDTTVWKTFLGACRSCGNIELACQVAGHLLEMEPEEHC 540
DLYGRSGRLEEAKALIEEMPFKPDTTVWKTFLGACRSCGNIELACQVAGHLLEMEPEEHC
Sbjct: 550 DLYGRSGRLEEAKALIEEMPFKPDTTVWKTFLGACRSCGNIELACQVAGHLLEMEPEEHC 609
Query: 541 TYVLLSNMYGNLMRWDEKAKVKRLMKERGVKKVPGWSWIEVNNNVHAFIAQDHSHPSCQQ 600
TYVLLSNMYGNLMRWDEKAKVKRLMKERGVKKVPGWSWIEVNNNVHAFIAQDHSHPSCQQ
Sbjct: 610 TYVLLSNMYGNLMRWDEKAKVKRLMKERGVKKVPGWSWIEVNNNVHAFIAQDHSHPSCQQ 669
Query: 601 IYFLLEVLLEEITRMEDAD 620
IYFLLEVLLEEITRMEDAD
Sbjct: 670 IYFLLEVLLEEITRMEDAD 688
BLAST of Cucsa.162960 vs. NCBI nr
Match:
gi|449442142|ref|XP_004138841.1| (PREDICTED: putative pentatricopeptide repeat-containing protein At3g25970 [Cucumis sativus])
HSP 1 Score: 126.3 bits (316), Expect = 1.8e-25
Identity = 76/270 (28.15%), Postives = 132/270 (48.89%), Query Frame = 1
Query: 256 HGLVIKRGFEQSVPISNALISMYLKSDYGSMKEALCIFESLEFKDRVSWNSILTGLSQTG 315
H L +K G V N +++ Y K ++ A +F+ + +D VSWN+++ G G
Sbjct: 21 HSLAVKLGTIADVYTCNNILNGYWKCK--ELRSADVLFDEMPMRDSVSWNTMIAGHINCG 80
Query: 316 SSEDAVKSFLHMRSAAMDIDHYSFSAVLRSCSDLATFQLGQQIHVLALKYGLESNEFVSS 375
+ E + MRS ++D Y+F ++L+ + F LGQQ+H + +K G N + S
Sbjct: 81 NLEASWDVLRCMRSCGFELDRYTFGSMLKGIAFAGMFHLGQQVHSIIIKMGYAENVYAGS 140
Query: 376 SLIFMYSKCGIIEDARRSFEEASKNSSITWNALMFGYAQHGQCNVALDLFFLMEEKKVKM 435
+L+ MY+KC +EDA SF SK+++++WNA++ GYAQ G A L ME++ K+
Sbjct: 141 ALLDMYAKCEKLEDAYLSFLSISKHNTVSWNAMINGYAQAGDRETAFWLLDCMEQEGEKV 200
Query: 436 DHITFVAV-----------LTACSHIGLVEQGCKFLRCMESDYGVPPRMEHYACAVDLYG 495
D T+ + LT+ H +++ G + + M + + Y
Sbjct: 201 DDGTYAPLLPLLDDADFCNLTSQLHGKIIKHGLELVNTMCN------------ALITSYS 260
Query: 496 RSGRLEEAKALIEEMPFKPDTTVWKTFLGA 515
+ G L++AK + + D W + L A
Sbjct: 261 KCGSLDDAKRIFDSSAGIRDLVTWNSLLAA 276
HSP 2 Score: 1199.5 bits (3102), Expect = 0.0e+00
Identity = 587/618 (94.98%), Postives = 603/618 (97.57%), Query Frame = 1
Query: 1 MIAGHINCGNLEASWDVLRCMRSCGFELDRYTFGSMLKGIAFAGMFHLGQQVHSIIIKMG 60
M+AGHINCGNLEASWDVL+CMR CGFE+DRYTFGSMLKGIAFAGMF LGQQVHS+IIKM
Sbjct: 70 MVAGHINCGNLEASWDVLKCMRRCGFEMDRYTFGSMLKGIAFAGMFDLGQQVHSMIIKMD 129
Query: 61 YAENVYAGSALLDMYAKCEKLEDAYLSFLSISKHNTVSWNAMINGYAQAGDRETAFWLLD 120
YA NVYAGSALLDMYAKCE+LEDAYLSFLSISK NTVSWNAMI GYAQAGDRETAFWLLD
Sbjct: 130 YAGNVYAGSALLDMYAKCERLEDAYLSFLSISKKNTVSWNAMIAGYAQAGDRETAFWLLD 189
Query: 121 CMEQEGEKVDDGTYAPLLPLLDDADFCNLTSQLHGKIIKHGLELVNTMCNALITSYSKCG 180
CMEQEGEKVDDGTYAPLLPLLDDADFCNLTSQLHGKIIKHGLE VNTMCNALITSYSKCG
Sbjct: 190 CMEQEGEKVDDGTYAPLLPLLDDADFCNLTSQLHGKIIKHGLEFVNTMCNALITSYSKCG 249
Query: 181 SLDDAKRIFDSSAGIRDLVTWNSLLAAYLLRSQEDLAFKLLIDMQEHGFEPDLYSYTSII 240
SLDDAKRIFDSSAGI DLVTWNSLLAAYLLRS+EDLAFKLLIDMQEHGFEPDLYSYTSII
Sbjct: 250 SLDDAKRIFDSSAGIWDLVTWNSLLAAYLLRSREDLAFKLLIDMQEHGFEPDLYSYTSII 309
Query: 241 SACFNENISNNGRSLHGLVIKRGFEQSVPISNALISMYLKSDYGSMKEALCIFESLEFKD 300
SACFNE +SNNG+SLHGLVIKRGFEQSVPISNALISMYLKSDYGSMKEALCIFESLEFKD
Sbjct: 310 SACFNEKLSNNGKSLHGLVIKRGFEQSVPISNALISMYLKSDYGSMKEALCIFESLEFKD 369
Query: 301 RVSWNSILTGLSQTGSSEDAVKSFLHMRSAAMDIDHYSFSAVLRSCSDLATFQLGQQIHV 360
RVSWNSILTGLSQTG SEDAVKSFL+MRSAAMDIDHYSFSAVLRSCSDLATFQLGQQIHV
Sbjct: 370 RVSWNSILTGLSQTGLSEDAVKSFLYMRSAAMDIDHYSFSAVLRSCSDLATFQLGQQIHV 429
Query: 361 LALKYGLESNEFVSSSLIFMYSKCGIIEDARRSFEEASKNSSITWNALMFGYAQHGQCNV 420
LALKYGLES+EFVSSSLIFMYSKCG IEDARRSFEEASKNSSITWNALMFGYAQHGQCNV
Sbjct: 430 LALKYGLESDEFVSSSLIFMYSKCGFIEDARRSFEEASKNSSITWNALMFGYAQHGQCNV 489
Query: 421 ALDLFFLMEEKKVKMDHITFVAVLTACSHIGLVEQGCKFLRCMESDYGVPPRMEHYACAV 480
ALDLFFLMEEK+VKMDHITFVAVLTACSHIGLVEQG KFL+CMESDYGVPPRMEHYACAV
Sbjct: 490 ALDLFFLMEEKRVKMDHITFVAVLTACSHIGLVEQGRKFLQCMESDYGVPPRMEHYACAV 549
Query: 481 DLYGRSGRLEEAKALIEEMPFKPDTTVWKTFLGACRSCGNIELACQVAGHLLEMEPEEHC 540
DLYGRSG LEEAKALIEEMPFKPD TVWKTFLGACRSCGN+ELACQVA HLLE+EPEEHC
Sbjct: 550 DLYGRSGHLEEAKALIEEMPFKPDVTVWKTFLGACRSCGNVELACQVASHLLEIEPEEHC 609
Query: 541 TYVLLSNMYGNLMRWDEKAKVKRLMKERGVKKVPGWSWIEVNNNVHAFIAQDHSHPSCQQ 600
TYVLLSNMYGNLMRW+EKAKVKRLMKERGVKKVPGWSWIEVNNNVHAFIAQDHSHPSC+Q
Sbjct: 610 TYVLLSNMYGNLMRWEEKAKVKRLMKERGVKKVPGWSWIEVNNNVHAFIAQDHSHPSCRQ 669
Query: 601 IYFLLEVLLEEITRMEDA 619
IYFLLEVLLEEITRMEDA
Sbjct: 670 IYFLLEVLLEEITRMEDA 687
BLAST of Cucsa.162960 vs. NCBI nr
Match:
gi|659089006|ref|XP_008445278.1| (PREDICTED: putative pentatricopeptide repeat-containing protein At3g25970 [Cucumis melo])
HSP 1 Score: 122.9 bits (307), Expect = 2.0e-24
Identity = 85/332 (25.60%), Postives = 155/332 (46.69%), Query Frame = 1
Query: 256 HGLVIKRGFEQSVPISNALISMYLKSDYGSMKEALCIFESLEFKDRVSWNSILTGLSQTG 315
H L +K G V N +++ Y K ++ A +F+ + +D VSWN+++ G G
Sbjct: 21 HSLAVKLGTIADVYTCNNILNGYWKCK--ELRSADILFDEMPLRDSVSWNTMVAGHINCG 80
Query: 316 SSEDAVKSFLHMRSAAMDIDHYSFSAVLRSCSDLATFQLGQQIHVLALKYGLESNEFVSS 375
+ E + MR ++D Y+F ++L+ + F LGQQ+H + +K N + S
Sbjct: 81 NLEASWDVLKCMRRCGFEMDRYTFGSMLKGIAFAGMFDLGQQVHSMIIKMDYAGNVYAGS 140
Query: 376 SLIFMYSKCGIIEDARRSFEEASKNSSITWNALMFGYAQHGQCNVALDLFFLMEEKKVKM 435
+L+ MY+KC +EDA SF SK ++++WNA++ GYAQ G A L ME++ K+
Sbjct: 141 ALLDMYAKCERLEDAYLSFLSISKKNTVSWNAMIAGYAQAGDRETAFWLLDCMEQEGEKV 200
Query: 436 DHITFVAV-----------LTACSHIGLVEQGCKFLRCMESDYGVPPRMEHYACAVDLYG 495
D T+ + LT+ H +++ G +F+ M + + Y
Sbjct: 201 DDGTYAPLLPLLDDADFCNLTSQLHGKIIKHGLEFVNTMCN------------ALITSYS 260
Query: 496 RSGRLEEAKALIEEMPFKPDTTVWKTFLGACRSCGNIELACQVAGHLLE--MEPEEHCTY 555
+ G L++AK + + D W + L A +LA ++ + E EP+ +
Sbjct: 261 KCGSLDDAKRIFDSSAGIWDLVTWNSLLAAYLLRSREDLAFKLLIDMQEHGFEPDLYSYT 320
Query: 556 VLLSNMYGNLMRWDEKAKVKRLMKERGVKKVP 575
++S + + + K+ ++K + VP
Sbjct: 321 SIISACFNEKLSNNGKSLHGLVIKRGFEQSVP 338
HSP 2 Score: 901.4 bits (2328), Expect = 9.0e-259
Identity = 434/614 (70.68%), Postives = 517/614 (84.20%), Query Frame = 1
Query: 1 MIAGHINCGNLEASWDVLRCMRSCGFELDRYTFGSMLKGIAFAGMFHLGQQVHSIIIKMG 60
MIAG++NCGN +W+ LR MR GFELD YTFGS+LKGIA + + +G++VHS+IIKMG
Sbjct: 71 MIAGYVNCGNFGIAWEFLRNMRRSGFELDGYTFGSILKGIAGSHQWDIGEEVHSMIIKMG 130
Query: 61 YAENVYAGSALLDMYAKCEKLEDAYLSFLSISKHNTVSWNAMINGYAQAGDRETAFWLLD 120
YA NVY+GSALLDMYAKCE++E+AY+ F + + NTVSWNA+I G+ Q GDR TAFWL
Sbjct: 131 YAGNVYSGSALLDMYAKCERVEEAYVVFEHMPERNTVSWNALIAGFVQVGDRRTAFWLFG 190
Query: 121 CMEQEGEKVDDGTYAPLLPLLDDADFCNLTSQLHGKIIKHGLELVNTMCNALITSYSKCG 180
CME++ K DDGT APLL LLDD++F T Q+HGKI K GLE NT+CNA+ITSYS+CG
Sbjct: 191 CMEKDAVKPDDGTIAPLLTLLDDSEFYWTTMQIHGKITKLGLEFSNTVCNAIITSYSECG 250
Query: 181 SLDDAKRIFDSSAGIRDLVTWNSLLAAYLLRSQEDLAFKLLIDMQEHGFEPDLYSYTSII 240
S+++AK++FD S RD+VTWNS+LAAYL+ +E LAF + +DMQ GFEPD+YSYTSI+
Sbjct: 251 SIENAKKVFDRSFDTRDVVTWNSMLAAYLIHGKEGLAFNIFMDMQWLGFEPDIYSYTSIV 310
Query: 241 SACFNENISNNGRSLHGLVIKRGFEQSVPISNALISMYLKSDYGSMKEALCIFESLEFKD 300
SACF E N G+SLHGL+IKRG EQSVPI+NALI+MYLKS SM+EAL IFE LE KD
Sbjct: 311 SACFEEAHKNLGQSLHGLIIKRGLEQSVPIANALIAMYLKSINKSMEEALHIFECLEMKD 370
Query: 301 RVSWNSILTGLSQTGSSEDAVKSFLHMRSAAMDIDHYSFSAVLRSCSDLATFQLGQQIHV 360
+VSWNSILTGLSQ G SEDA+K F+HMR A++IDHY+ SAV+RSCSDLAT QLGQQ+HV
Sbjct: 371 KVSWNSILTGLSQFGLSEDALKFFVHMRYVAVEIDHYTLSAVIRSCSDLATLQLGQQVHV 430
Query: 361 LALKYGLESNEFVSSSLIFMYSKCGIIEDARRSFEEASKNSSITWNALMFGYAQHGQCNV 420
LALK GLESNEFV+SSLIFMY+KCGIIEDAR+SFEE +S ITWN+L+FGYAQHG +
Sbjct: 431 LALKSGLESNEFVASSLIFMYAKCGIIEDARKSFEENPNDSPITWNSLIFGYAQHGLGYI 490
Query: 421 ALDLFFLMEEKKVKMDHITFVAVLTACSHIGLVEQGCKFLRCMESDYGVPPRMEHYACAV 480
ALD+FF ME++KVK+DHITFVAVLTACSHIGLVEQGC+ L+ MES+YG+ PRMEHYACAV
Sbjct: 491 ALDIFFEMEKRKVKLDHITFVAVLTACSHIGLVEQGCELLKSMESNYGITPRMEHYACAV 550
Query: 481 DLYGRSGRLEEAKALIEEMPFKPDTTVWKTFLGACRSCGNIELACQVAGHLLEMEPEEHC 540
DLYGR+GRL EAKALIE MPF+PD VWKTFLGACR+CGNIELA QVA LL++EPEEHC
Sbjct: 551 DLYGRAGRLNEAKALIETMPFEPDAIVWKTFLGACRACGNIELASQVASRLLDLEPEEHC 610
Query: 541 TYVLLSNMYGNLMRWDEKAKVKRLMKERGVKKVPGWSWIEVNNNVHAFIAQDHSHPSCQQ 600
TYVLLS+MYG L RWDEKA VKRLMKERGVKKVPGWSWIE+ N VHAF A+D HP+C +
Sbjct: 611 TYVLLSDMYGYLRRWDEKASVKRLMKERGVKKVPGWSWIEIKNQVHAFKAEDRLHPNCGE 670
Query: 601 IYFLLEVLLEEITR 615
IYF+L L++EI+R
Sbjct: 671 IYFVLGGLMDEISR 684
BLAST of Cucsa.162960 vs. NCBI nr
Match:
gi|1009134051|ref|XP_015884237.1| (PREDICTED: putative pentatricopeptide repeat-containing protein At3g25970 [Ziziphus jujuba])
HSP 1 Score: 147.5 bits (371), Expect = 7.6e-32
Identity = 104/384 (27.08%), Postives = 187/384 (48.70%), Query Frame = 1
Query: 154 HGKIIKHGLELVNTMCNALITSYSKCGSLDDAKRIFDSSAGIRDLVTWNSLLAAYLLRSQ 213
H +K G + N ++ YS+ A ++FD RD V+WN+++A Y+
Sbjct: 22 HSHAVKSGTIADIYIANNILNGYSRNQQFGLAHKLFDKMLN-RDTVSWNTMIAGYVNCGN 81
Query: 214 EDLAFKLLIDMQEHGFEPDLYSYTSIISACFNENISNNGRSLHGLVIKRGFEQSVPISNA 273
+A++ L +M+ GFE D Y++ SI+ + + G +H ++IK G+ +V +A
Sbjct: 82 FGIAWEFLRNMRRSGFELDGYTFGSILKGIAGSHQWDIGEEVHSMIIKMGYAGNVYSGSA 141
Query: 274 LISMYLKSDYGSMKEALCIFESLEFKDRVSWNSILTGLSQTGSSEDAVKSFLHMRSAAMD 333
L+ MY K + ++EA +FE + ++ VSWN+++ G Q G A F M A+
Sbjct: 142 LLDMYAKCE--RVEEAYVVFEHMPERNTVSWNALIAGFVQVGDRRTAFWLFGCMEKDAVK 201
Query: 334 IDHYSFSAVLRSCSDLATFQLGQQIHVLALKYGLESNEFVSSSLIFMYSKCGIIEDARRS 393
D + + +L D + QIH K GLE + V +++I YS+CG IE+A++
Sbjct: 202 PDDGTIAPLLTLLDDSEFYWTTMQIHGKITKLGLEFSNTVCNAIITSYSECGSIENAKKV 261
Query: 394 FEEA-SKNSSITWNALMFGYAQHGQCNVALDLFFLMEEKKVKMDHITFVAVLTAC----- 453
F+ + +TWN+++ Y HG+ +A ++F M+ + D ++ ++++AC
Sbjct: 262 FDRSFDTRDVVTWNSMLAAYLIHGKEGLAFNIFMDMQWLGFEPDIYSYTSIVSACFEEAH 321
Query: 454 SHIGLVEQGCKFLRCMESDYGVPPRMEHYACAVDLYGRSGRLEEAKALIEEMPFKPDTTV 513
++G G R +E VP + + A+ L + +EEA + E + K D
Sbjct: 322 KNLGQSLHGLIIKRGLEQ--SVP--IANALIAMYLKSINKSMEEALHIFECLEMK-DKVS 381
Query: 514 WKTFLGACRSCGNIELACQVAGHL 532
W + L G E A + H+
Sbjct: 382 WNSILTGLSQFGLSEDALKFFVHM 397
HSP 2 Score: 900.6 bits (2326), Expect = 1.5e-258
Identity = 426/619 (68.82%), Postives = 521/619 (84.17%), Query Frame = 1
Query: 1 MIAGHINCGNLEASWDVLRCMRSCGFELDRYTFGSMLKGIAFAGMFHLGQQVHSIIIKMG 60
MIAG++NCGNLE ++++++ M+ CGF+ D YTFGS+LKG+A A +GQQ+HS+I+KMG
Sbjct: 72 MIAGYVNCGNLETAFEIMKDMKRCGFDFDGYTFGSLLKGVASAYRLQVGQQLHSMIVKMG 131
Query: 61 YAENVYAGSALLDMYAKCEKLEDAYLSFLSISKHNTVSWNAMINGYAQAGDRETAFWLLD 120
Y ENVYAGSALLDMYAKCEK+ DAY+ F + + N+VSWNA+I G++Q GDR T FWLLD
Sbjct: 132 YEENVYAGSALLDMYAKCEKVGDAYMVFECLPEPNSVSWNALIAGFSQMGDRSTVFWLLD 191
Query: 121 CMEQEGEKVDDGTYAPLLPLLDDADFCNLTSQLHGKIIKHGLELVNTMCNALITSYSKCG 180
CME+EG KVDDGTYAPLL LLDD +F LT Q+HGKIIK GL NT+CNA+ITSYS+CG
Sbjct: 192 CMEKEGVKVDDGTYAPLLTLLDDIEFYKLTIQIHGKIIKRGLACDNTVCNAMITSYSECG 251
Query: 181 SLDDAKRIFDSSAGIRDLVTWNSLLAAYLLRSQEDLAFKLLIDMQEHGFEPDLYSYTSII 240
S+ DA+++FD + G+RDLVTWNS+LAAYL+ +E+L FKL +DMQ GFEPD+Y+YTSI+
Sbjct: 252 SIGDARKVFDDAVGMRDLVTWNSMLAAYLVHEKEELGFKLFLDMQRLGFEPDIYTYTSIL 311
Query: 241 SACFNENISNNGRSLHGLVIKRGFEQSVPISNALISMYLKSDYGSMKEALCIFESLEFKD 300
SACF + ++G+S+H +VIKRG E SVPISNALI+MYLKS+ SM+EAL +FES+E KD
Sbjct: 312 SACFEKAHKSHGKSVHAVVIKRGLEYSVPISNALIAMYLKSNSTSMEEALSLFESMELKD 371
Query: 301 RVSWNSILTGLSQTGSSEDAVKSFLHMRSAAMDIDHYSFSAVLRSCSDLATFQLGQQIHV 360
RVSWNSILTG SQ G SEDA+ F MR ++IDHY+ SAVLRSCSDLAT QLG+Q+HV
Sbjct: 372 RVSWNSILTGFSQIGLSEDALNFFGKMRGFMVEIDHYALSAVLRSCSDLATLQLGRQVHV 431
Query: 361 LALKYGLESNEFVSSSLIFMYSKCGIIEDARRSFEEASKNSSITWNALMFGYAQHGQCNV 420
LA+K G E+N+FV+S+LIFMYSKCGII+DAR+SFEE K+ SI WN+++FGYAQ+GQ N
Sbjct: 432 LAIKLGFETNDFVASALIFMYSKCGIIQDARKSFEETPKDISIAWNSIIFGYAQNGQGND 491
Query: 421 ALDLFFLMEEKKVKMDHITFVAVLTACSHIGLVEQGCKFLRCMESDYGVPPRMEHYACAV 480
ALDLFFLM + KV++DHITFVAVLTACSHIGLVE+G FL+ MESDYG+PPRMEHYACAV
Sbjct: 492 ALDLFFLMRDTKVRLDHITFVAVLTACSHIGLVEEGLNFLKSMESDYGIPPRMEHYACAV 551
Query: 481 DLYGRSGRLEEAKALIEEMPFKPDTTVWKTFLGACRSCGNIELACQVAGHLLEMEPEEHC 540
DL+GR+GRL+EAK LIE MPFKPD VWKT LGACR CG+IELA QVA HLL++EPEEHC
Sbjct: 552 DLFGRAGRLDEAKPLIESMPFKPDAMVWKTLLGACRVCGDIELAAQVASHLLDLEPEEHC 611
Query: 541 TYVLLSNMYGNLMRWDEKAKVKRLMKERGVKKVPGWSWIEVNNNVHAFIAQDHSHPSCQQ 600
TYV+LSNMYG+L RW EKA V RLM+ERGVKKVPGWSWIE+ N VHAF A+D SHP C++
Sbjct: 612 TYVILSNMYGHLRRWGEKASVTRLMRERGVKKVPGWSWIEIKNQVHAFNAEDQSHPHCKE 671
Query: 601 IYFLLEVLLEEITRMEDAD 620
IY +L L+EEIT + DAD
Sbjct: 672 IYQMLGGLMEEITWL-DAD 689
BLAST of Cucsa.162960 vs. NCBI nr
Match:
gi|590571697|ref|XP_007011666.1| (Pentatricopeptide repeat-containing protein, putative [Theobroma cacao])
HSP 1 Score: 212.2 bits (539), Expect = 2.5e-51
Identity = 141/486 (29.01%), Postives = 245/486 (50.41%), Query Frame = 1
Query: 30 RYTFGSMLKGIAFAGMFHLGQQVHSIIIKMGYAENVYAGSALLDMYAKCEKLEDAYLSFL 89
R S+L+ A+A F+ H IK+G +VY + +L+ YA+C++L A F
Sbjct: 2 RRPLNSLLESSAYA--FYKVLTTHCCAIKLGTLADVYTANKILNAYARCKELHVARKLFA 61
Query: 90 SISKHNTVSWNAMINGYAQAGDRETAFWLLDCMEQEGEKVDDGTYAPLLPLLDDADFCNL 149
+ +TVSWN MI GY G+ ETAF ++ M++ G D T+ LL + A +
Sbjct: 62 EVLHRDTVSWNTMIAGYVNCGNLETAFEIMKDMKRCGFDFDGYTFGSLLKGVASAYRLQV 121
Query: 150 TSQLHGKIIKHGLELVNTMCNALITSYSKCGSLDDAKRIFDSSAGIRDLVTWNSLLAAYL 209
QLH I+K G E +AL+ Y+KC + DA +F+ + V+WN+L+A +
Sbjct: 122 GQQLHSMIVKMGYEENVYAGSALLDMYAKCEKVGDAYMVFECLPE-PNSVSWNALIAGFS 181
Query: 210 LRSQEDLAFKLLIDMQEHGFEPDLYSYTSIISACFNENISNNGRSLHGLVIKRGFEQSVP 269
F LL M++ G + D +Y +++ + +HG +IKRG
Sbjct: 182 QMGDRSTVFWLLDCMEKEGVKVDDGTYAPLLTLLDDIEFYKLTIQIHGKIIKRGLACDNT 241
Query: 270 ISNALISMYLKSDYGSMKEALCIF-ESLEFKDRVSWNSILTGLSQTGSSEDAVKSFLHMR 329
+ NA+I+ Y S+ GS+ +A +F +++ +D V+WNS+L E K FL M+
Sbjct: 242 VCNAMITSY--SECGSIGDARKVFDDAVGMRDLVTWNSMLAAYLVHEKEELGFKLFLDMQ 301
Query: 330 SAAMDIDHYSFSAVLRSCSDLATFQLGQQIHVLALKYGLESNEFVSSSLIFMYSKCG--I 389
+ D Y+++++L +C + A G+ +H + +K GLE + +S++LI MY K
Sbjct: 302 RLGFEPDIYTYTSILSACFEKAHKSHGKSVHAVVIKRGLEYSVPISNALIAMYLKSNSTS 361
Query: 390 IEDARRSFEEASKNSSITWNALMFGYAQHGQCNVALDLFFLMEEKKVKMDHITFVAVLTA 449
+E+A FE ++WN+++ G++Q G AL+ F M V++DH AVL +
Sbjct: 362 MEEALSLFESMELKDRVSWNSILTGFSQIGLSEDALNFFGKMRGFMVEIDHYALSAVLRS 421
Query: 450 CSHIGLVEQGCKFLRCMESDYGVPPRMEHYACAVDLYGRSGRLEEAKALIEEMPFKPDTT 509
CS + ++ G + + + G + + +Y + G +++A+ EE P K +
Sbjct: 422 CSDLATLQLG-RQVHVLAIKLGFETNDFVASALIFMYSKCGIIQDARKSFEETP-KDISI 480
Query: 510 VWKTFL 513
W + +
Sbjct: 482 AWNSII 480
HSP 2 Score: 890.6 bits (2300), Expect = 1.6e-255
Identity = 429/616 (69.64%), Postives = 519/616 (84.25%), Query Frame = 1
Query: 1 MIAGHINCGNLEASWDVLRCMRSCGFELDRYTFGSMLKGIAFAGMFHLGQQVHSIIIKMG 60
MIAGH+N GN E +W++LR M+ CGF+ D YTFGS+LKG+A A LG+QVHS+++K G
Sbjct: 1 MIAGHVNYGNFETAWEILREMKGCGFDFDGYTFGSILKGVACAYRCDLGEQVHSMVVKTG 60
Query: 61 YAENVYAGSALLDMYAKCEKLEDAYLSFLSISKHNTVSWNAMINGYAQAGDRETAFWLLD 120
Y NVY+GSALLDMYAKC ++E+AY+ F S+ + N+VSWNA+I G+ Q GDR+TAFWL++
Sbjct: 61 YVGNVYSGSALLDMYAKCGRVEEAYVVFDSLPERNSVSWNALIAGFVQVGDRKTAFWLMN 120
Query: 121 CMEQEGEKVDDGTYAPLLPLLDDADFCNLTSQLHGKIIKHGLELVNTMCNALITSYSKCG 180
CMEQEG K+DDG AP+L LLDDA+F T Q+H KI+KHGL+ +T+CNA ITSYS+CG
Sbjct: 121 CMEQEGVKLDDGAIAPILTLLDDAEFYKSTMQIHCKILKHGLDCKSTVCNATITSYSECG 180
Query: 181 SLDDAKRIFDSSAGIRDLVTWNSLLAAYLLRSQEDLAFKLLIDMQEHGFEPDLYSYTSII 240
S++DA+ +FD + G RDLVTWNS+LAAYL+ +E LA KL IDMQ GFEPD+YSYTSII
Sbjct: 181 SIEDAETVFDGAFGSRDLVTWNSMLAAYLIHKKELLACKLFIDMQMLGFEPDIYSYTSII 240
Query: 241 SACFNENISNNGRSLHGLVIKRGFEQSVPISNALISMYLKSDYGSMKEALCIFESLEFKD 300
SA + N+G+S HGLVIKRG E+S PISNALI+ YLKS+ SM+EAL IF+S+E KD
Sbjct: 241 SAYSEDVHKNHGKSYHGLVIKRGLEKSEPISNALIARYLKSNSNSMEEALQIFKSMESKD 300
Query: 301 RVSWNSILTGLSQTGSSEDAVKSFLHMRSAAMDIDHYSFSAVLRSCSDLATFQLGQQIHV 360
RVSWNSILTGLSQ G +E A+K F HMR A +DIDHY+FSAVLRSCSDLAT QLGQ+IH
Sbjct: 301 RVSWNSILTGLSQMGLNEYALKFFGHMRYAEVDIDHYAFSAVLRSCSDLATLQLGQEIHA 360
Query: 361 LALKYGLESNEFVSSSLIFMYSKCGIIEDARRSFEEASKNSSITWNALMFGYAQHGQCNV 420
LA K GLESNEFV+SSLIFMY+KCGIIEDAR+SFE+ K+SSITWN+++FGYAQHGQ V
Sbjct: 361 LAHKSGLESNEFVASSLIFMYTKCGIIEDARKSFEQTPKDSSITWNSIIFGYAQHGQGYV 420
Query: 421 ALDLFFLMEEKKVKMDHITFVAVLTACSHIGLVEQGCKFLRCMESDYGVPPRMEHYACAV 480
ALDLFF M+++KVK+DHITFVAVLTACSH+GLVEQGCKFL MESDYG+ PRMEHYACAV
Sbjct: 421 ALDLFFQMKKEKVKLDHITFVAVLTACSHMGLVEQGCKFLMSMESDYGITPRMEHYACAV 480
Query: 481 DLYGRSGRLEEAKALIEEMPFKPDTTVWKTFLGACRSCGNIELACQVAGHLLEMEPEEHC 540
DLYGR+GRL+EAKALIE MPFKPD V +T LGACR+CGNIELA Q+AG+LLE+EPEEHC
Sbjct: 481 DLYGRAGRLDEAKALIEAMPFKPDAMVLRTLLGACRACGNIELASQIAGYLLELEPEEHC 540
Query: 541 TYVLLSNMYGNLMRWDEKAKVKRLMKERGVKKVPGWSWIEVNNNVHAFIAQDHSHPSCQQ 600
TYVLLS+MYG+L RWDEKA VKRLM+ERGV+KVPGWSWIE+ N V +F A+D SH C++
Sbjct: 541 TYVLLSDMYGHLKRWDEKASVKRLMRERGVRKVPGWSWIEIKNEVFSFKAEDRSHSHCEE 600
Query: 601 IYFLLEVLLEEITRME 617
IY +L VL+EEI RM+
Sbjct: 601 IYCVLGVLMEEIKRMD 616
The following BLAST results are available for this feature:
| Match Name | E-value | Identity | Description | |
|---|
| PP255_ARATH | 8.0e-207 | 56.51 | Putative pentatricopeptide repeat-containing protein At3g25970 OS=Arabidopsis th... | [more] |
| PP307_ARATH | 1.1e-118 | 36.88 | Pentatricopeptide repeat-containing protein At4g13650 OS=Arabidopsis thaliana GN... | [more] |
| PP172_ARATH | 2.8e-111 | 36.12 | Pentatricopeptide repeat-containing protein At2g27610 OS=Arabidopsis thaliana GN... | [more] |
| PP333_ARATH | 3.2e-107 | 35.71 | Pentatricopeptide repeat-containing protein At4g21300 OS=Arabidopsis thaliana GN... | [more] |
| PP151_ARATH | 7.5e-104 | 34.66 | Pentatricopeptide repeat-containing protein At2g13600 OS=Arabidopsis thaliana GN... | [more] |
| Match Name | E-value | Identity | Description | |
|---|
| A0A0A0LMK6_CUCSA | 0.0e+00 | 100.00 | Uncharacterized protein OS=Cucumis sativus GN=Csa_2G382810 PE=4 SV=1 | [more] |
| A0A0A0LMK6_CUCSA | 1.3e-25 | 28.15 | Uncharacterized protein OS=Cucumis sativus GN=Csa_2G382810 PE=4 SV=1 | [more] |
| A0A061GHG1_THECC | 1.8e-51 | 29.01 | Pentatricopeptide repeat-containing protein, putative OS=Theobroma cacao GN=TCM_... | [more] |
| W9S7C5_9ROSA | 7.2e-255 | 68.39 | Uncharacterized protein OS=Morus notabilis GN=L484_007144 PE=4 SV=1 | [more] |
| W9S7C5_9ROSA | 5.3e-24 | 27.27 | Uncharacterized protein OS=Morus notabilis GN=L484_007144 PE=4 SV=1 | [more] |
| Match Name | E-value | Identity | Description | |
|---|
| AT3G25970.1 | 4.5e-208 | 56.51 | Pentatricopeptide repeat (PPR) superfamily protein | [more] |
| AT4G13650.1 | 6.1e-120 | 36.88 | Pentatricopeptide repeat (PPR) superfamily protein | [more] |
| AT2G27610.1 | 1.6e-112 | 36.12 | Tetratricopeptide repeat (TPR)-like superfamily protein | [more] |
| AT4G21300.1 | 1.8e-108 | 35.71 | Tetratricopeptide repeat (TPR)-like superfamily protein | [more] |
| AT2G13600.1 | 4.2e-105 | 34.66 | Pentatricopeptide repeat (PPR) superfamily protein | [more] |
