Cucsa.162820 (gene) Cucumber (Gy14) v1 | Cucurbit Genomics Database (CuGenDB)

Overview


Name	Cucsa.162820
Type	gene
Organism	Cucumis sativus (Cucumber (Gy14) v1)
Description	WRKY transcription factor
Location	scaffold01144 : 2350607 .. 2351535 (+)

Genome Viewer

Gene Expression

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDS

Hold the cursor over a type above to highlight its positions in the sequence below.

GTGGAATGTCTCCAAACAGAGCTAGAAAAGTTGAGGAAAGAGAATGAAGCTCTGAAGTTGATGCTGAAAGTTGTGAGCATCAAGAACTTGGTATCTCAAGTGGATGTTTGTTCAAGAGATCATCGTCATGATCAGAATGACGAGGGTTCAAGATCAGAGAGAGCCAATCTCAAAGTTAGTCCACCCGCTCTTGCTCTTGCTCTCGAAACTTCGTCCACCACCCAAGCATATGCTAGAACTACCTTCAAAGACCAAGCTTTGGTATGGAAATTTAATAGTAGAAATTAGTTTTCCTGTGATCTCCCTCTTCTTTTGTTTTTAATGAAAAATTTCGATGCCATGTATTTATTTGGGTTGAATTATAAAGATGGTGAAGGATGGATATAAATGGAGGAAATATGGGCAAAAGATTACCAAGGATAATCAATCTCCTCGTGCTTATTTCAAGTGTTCTTCCCCAGGATGCCCTGTCAAAAAAAaGGTAaTTTATTCCAATTTCTAAATTACAAAGTTTTAAATTTGAAACTTTACTATGTTTTGATTCATTACACTATAATGGTATCAATTTAATGTTCTTACTCTTATTTAGTTTGAAAGTTATATATTAGATTACAATTGTAATTGTAGTATACCTAAATAAGACCATGATGATGATCAGAATTCAGAATTCAGTATCTTGGGGATTACATTAAATGATTTCTCCTACACTTTTTAGTTTTCTATAATAATCAATGGAATTGTTATAAATAGTTAATAGAATTAAAATGTGAAATGGTGTTGAAATTTAGGTACAAAGAAGCCTGGAAAATAAGTCAATGGTGATAGTCACATACGATGGCCACCACAACCACAACCACAACCACGAAAATGCCTCTCCTCCACCGCTTTCAAGCTCTCAACGAGGGTCGTCTTCGTCACCACCGCTTCCG

mRNA sequence

GTGGAATGTCTCCAAACAGAGCTAGAAAAGTTGAGGAAAGAGAATGAAGCTCTGAAGTTGATGCTGAAAGTTGTGAGCATCAAGAACTTGGTATCTCAAGTGGATGTTTGTTCAAGAGATCATCGTCATGATCAGAATGACGAGGGTTCAAGATCAGAGAGAGCCAATCTCAAAGTTAGTCCACCCGCTCTTGCTCTTGCTCTCGAAACTTCGTCCACCACCCAAGCATATGCTAGAACTACCTTCAAAGACCAAGCTTTGATGGTGAAGGATGGATATAAATGGAGGAAATATGGGCAAAAGATTACCAAGGATAATCAATCTCCTCGTGCTTATTTCAAGTGTTCTTCCCCAGGATGCCCTGTCAAAAAAAAGGTACAAAGAAGCCTGGAAAATAAGTCAATGGTGATAGTCACATACGATGGCCACCACAACCACAACCACAACCACGAAAATGCCTCTCCTCCACCGCTTTCAAGCTCTCAACGAGGGTCGTCTTCGTCACCACCGCTTCCG

Coding sequence (CDS)

GTGGAATGTCTCCAAACAGAGCTAGAAAAGTTGAGGAAAGAGAATGAAGCTCTGAAGTTGATGCTGAAAGTTGTGAGCATCAAGAACTTGGTATCTCAAGTGGATGTTTGTTCAAGAGATCATCGTCATGATCAGAATGACGAGGGTTCAAGATCAGAGAGAGCCAATCTCAAAGTTAGTCCACCCGCTCTTGCTCTTGCTCTCGAAACTTCGTCCACCACCCAAGCATATGCTAGAACTACCTTCAAAGACCAAGCTTTGATGGTGAAGGATGGATATAAATGGAGGAAATATGGGCAAAAGATTACCAAGGATAATCAATCTCCTCGTGCTTATTTCAAGTGTTCTTCCCCAGGATGCCCTGTCAAAAAAAaGGTACAAAGAAGCCTGGAAAATAAGTCAATGGTGATAGTCACATACGATGGCCACCACAACCACAACCACAACCACGAAAATGCCTCTCCTCCACCGCTTTCAAGCTCTCAACGAGGGTCGTCTTCGTCACCACCGCTTCCG

Protein sequence

VECLQTELEKLRKENEALKLMLKVVSIKNLVSQVDVCSRDHRHDQNDEGSRSERANLKVSPPALALALETSSTTQAYARTTFKDQALMVKDGYKWRKYGQKITKDNQSPRAYFKCSSPGCPVKKKVQRSLENKSMVIVTYDGHHNHNHNHENASPPPLSSSQRGSSSSPPLP

Homology

[Close]

BLAST of Cucsa.162820 vs. Swiss-Prot
Match: WRK60_ARATH (Probable WRKY transcription factor 60 OS=Arabidopsis thaliana GN=WRKY60 PE=1 SV=1)

HSP 1 Score: 114.0 bits (284), Expect = 1.6e-24
Identity = 70/168 (41.67%), Postives = 99/168 (58.93%), Query Frame = 1


Query: 4   LQTELEKLRKENEALKLML-----KVVSIKNLVSQVDVC-SRDHRHDQNDE--GSRSERA 63
           LQ E+ ++  EN+ L  ML     K  ++ NL+ ++    S +  + QN +  G R +  
Sbjct: 45  LQDEINRVNSENKKLTEMLARVCEKYYALNNLMEELQSRKSPESVNFQNKQLTGKRKQEL 104

Query: 64  NLKVSPPALALAL--------ETSSTTQAYARTTFKDQALMVKDGYKWRKYGQKITKDNQ 123
           +  VS P + L+L        + ++ + AY      D +L VKDGY+WRKYGQKIT+DN 
Sbjct: 105 DEFVSSP-IGLSLGPIENITNDKATVSTAYFAAEKSDTSLTVKDGYQWRKYGQKITRDNP 164

Query: 124 SPRAYFKCS-SPGCPVKKKVQRSLENKSMVIVTYDGHHNHNHNHENAS 155
           SPRAYF+CS SP C VKKKVQRS E+ S ++ TY+G HNH   H + S
Sbjct: 165 SPRAYFRCSFSPSCLVKKKVQRSAEDPSFLVATYEGTHNHTGPHASVS 211

[Close]

BLAST of Cucsa.162820 vs. Swiss-Prot
Match: WRK18_ARATH (WRKY transcription factor 18 OS=Arabidopsis thaliana GN=WRKY18 PE=1 SV=2)

HSP 1 Score: 109.4 bits (272), Expect = 4.0e-23
Identity = 60/167 (35.93%), Postives = 97/167 (58.08%), Query Frame = 1


Query: 2   ECLQTELEKLRKENEALKLMLKVVSIKNLVSQVDVCSRDHRHDQNDEGSRSERANLKVSP 61
           E  QT++   +++ +  + +   + + +  ++    + DH H       +++  + K   
Sbjct: 91  EIEQTDIPIKKRKQDPDEFLGFPIGLSSGKTENSSSNEDHHHHHQQHEQKNQLLSCK--- 150

Query: 62  PALALALETSSTTQAYARTTFKDQALMVKDGYKWRKYGQKITKDNQSPRAYFKCS-SPGC 121
             +  +   +  +  Y  T   D +L VKDG++WRKYGQK+T+DN SPRAYF+CS +P C
Sbjct: 151 RPVTDSFNKAKVSTVYVPTETSDTSLTVKDGFQWRKYGQKVTRDNPSPRAYFRCSFAPSC 210

Query: 122 PVKKKVQRSLENKSMVIVTYDGHHNHNHNHENASPPPLSSSQRGSSS 168
           PVKKKVQRS E+ S+++ TY+G   HNH   NAS    ++SQ GSS+
Sbjct: 211 PVKKKVQRSAEDPSLLVATYEG--THNHLGPNASEGD-ATSQGGSST 251

[Close]

BLAST of Cucsa.162820 vs. Swiss-Prot
Match: WRK40_ARATH (Probable WRKY transcription factor 40 OS=Arabidopsis thaliana GN=WRKY40 PE=1 SV=1)

HSP 1 Score: 107.8 bits (268), Expect = 1.2e-22
Identity = 50/111 (45.05%), Postives = 76/111 (68.47%), Query Frame = 1


Query: 74  TQAYARTTFKDQALMVKDGYKWRKYGQKITKDNQSPRAYFKCS-SPGCPVKKKVQRSLEN 133
           ++ Y +T   D  L+VKDGY+WRKYGQK+T+DN SPRAYFKC+ +P C VKKKVQRS+E+
Sbjct: 130 SRVYYKTEASDTTLVVKDGYQWRKYGQKVTRDNPSPRAYFKCACAPSCSVKKKVQRSVED 189

Query: 134 KSMVIVTYDGHHNH--------------NHNHENASPPPLSSSQRGSSSSP 170
           +S+++ TY+G HNH              + +H  ++  P+++++R S + P
Sbjct: 190 QSVLVATYEGEHNHPMPSQIDSNNGLNRHISHGGSASTPVAANRRSSLTVP 240

[Close]

BLAST of Cucsa.162820 vs. Swiss-Prot
Match: WRKY4_ARATH (Probable WRKY transcription factor 4 OS=Arabidopsis thaliana GN=WRKY4 PE=1 SV=2)

HSP 1 Score: 94.7 bits (234), Expect = 1.0e-18
Identity = 43/126 (34.13%), Postives = 74/126 (58.73%), Query Frame = 1


Query: 44  DQNDEGSRSERANLKVSPPALALALETSSTTQAYARTTFKDQALMVKDGYKWRKYGQKIT 103
           D+N+   +     +++S PA A +  T +  +   +TT   +  ++ DGY+WRKYGQK+ 
Sbjct: 365 DENEPDPKRRSTEVRISEPAPAASHRTVTEPRIIVQTT--SEVDLLDDGYRWRKYGQKVV 424

Query: 104 KDNQSPRAYFKCSSPGCPVKKKVQRSLENKSMVIVTYDGHHNHNHNHENASPPPLSSSQR 163
           K N  PR+Y+KC++PGC V+K V+R+  +   V+ TY+G HNH+     +S    +++Q 
Sbjct: 425 KGNPYPRSYYKCTTPGCGVRKHVERAATDPKAVVTTYEGKHNHDLPAAKSSSHAAAAAQL 484

Query: 164 GSSSSP 170
              + P
Sbjct: 485 RPDNRP 488

HSP 2 Score: 81.6 bits (200), Expect = 8.9e-15
Identity = 47/99 (47.47%), Postives = 60/99 (60.61%), Query Frame = 1


Query: 52  SERANLKVSPPALALALETSSTTQAYARTTFKDQALMV----KDGYKWRKYGQKITKDNQ 111
           S +A +  S P L    ETS  T    R+    Q L V     DGY WRKYGQK  K ++
Sbjct: 191 SGQAQIPTSAP-LPAQRETSDVTIIEHRS---QQPLNVDKPADDGYNWRKYGQKQVKGSE 250

Query: 112 SPRAYFKCSSPGCPVKKKVQRSLENKSMVIVTYDGHHNH 147
            PR+Y+KC++PGCPVKKKV+RSL+ +   I+ Y G HNH
Sbjct: 251 FPRSYYKCTNPGCPVKKKVERSLDGQVTEII-YKGQHNH 284

[Close]

BLAST of Cucsa.162820 vs. Swiss-Prot
Match: WRK46_HORVU (WRKY transcription factor SUSIBA2 OS=Hordeum vulgare GN=WRKY46 PE=1 SV=1)

HSP 1 Score: 91.7 bits (226), Expect = 8.6e-18
Identity = 40/88 (45.45%), Postives = 59/88 (67.05%), Query Frame = 1


Query: 88  MVKDGYKWRKYGQKITKDNQSPRAYFKCSSPGCPVKKKVQRSLENKSMVIVTYDGHHNH- 147
           ++ DGY+WRKYGQK+ K N +PR+Y+KC+S GCPV+K V+R+  +   VI TY+G HNH 
Sbjct: 361 ILDDGYRWRKYGQKVVKGNPNPRSYYKCTSTGCPVRKHVERASHDPKSVITTYEGKHNHE 420

Query: 148 -----NHNHENASPPPLSSSQRGSSSSP 170
                N  HE ++PP  +   + +S+ P
Sbjct: 421 VPAARNATHEMSAPPMKNVVHQINSNMP 448

HSP 2 Score: 68.6 bits (166), Expect = 7.8e-11
Identity = 28/56 (50.00%), Postives = 40/56 (71.43%), Query Frame = 1


Query: 91  DGYKWRKYGQKITKDNQSPRAYFKCSSPGCPVKKKVQRSLENKSMVIVTYDGHHNH 147
           DGY WRKYGQK  K +++PR+Y+KC+ P C VKK ++R+++   +  V Y G HNH
Sbjct: 190 DGYNWRKYGQKHVKGSENPRSYYKCTHPNCEVKKLLERAVDG-LITEVVYKGRHNH 244

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BLAST of Cucsa.162820 vs. TrEMBL
Match: A0A0A0LMJ1_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_2G382660 PE=4 SV=1)

HSP 1 Score: 351.3 bits (900), Expect = 6.7e-94
Identity = 172/172 (100.00%), Postives = 172/172 (100.00%), Query Frame = 1


Query: 1   VECLQTELEKLRKENEALKLMLKVVSIKNLVSQVDVCSRDHRHDQNDEGSRSERANLKVS 60
           VECLQTELEKLRKENEALKLMLKVVSIKNLVSQVDVCSRDHRHDQNDEGSRSERANLKVS
Sbjct: 5   VECLQTELEKLRKENEALKLMLKVVSIKNLVSQVDVCSRDHRHDQNDEGSRSERANLKVS 64

Query: 61  PPALALALETSSTTQAYARTTFKDQALMVKDGYKWRKYGQKITKDNQSPRAYFKCSSPGC 120
           PPALALALETSSTTQAYARTTFKDQALMVKDGYKWRKYGQKITKDNQSPRAYFKCSSPGC
Sbjct: 65  PPALALALETSSTTQAYARTTFKDQALMVKDGYKWRKYGQKITKDNQSPRAYFKCSSPGC 124

Query: 121 PVKKKVQRSLENKSMVIVTYDGHHNHNHNHENASPPPLSSSQRGSSSSPPLP 173
           PVKKKVQRSLENKSMVIVTYDGHHNHNHNHENASPPPLSSSQRGSSSSPPLP
Sbjct: 125 PVKKKVQRSLENKSMVIVTYDGHHNHNHNHENASPPPLSSSQRGSSSSPPLP 176

[Close]

BLAST of Cucsa.162820 vs. TrEMBL
Match: S5CS62_JATCU (Uncharacterized protein OS=Jatropha curcas GN=WRKY29 PE=4 SV=1)

HSP 1 Score: 147.9 bits (372), Expect = 1.1e-32
Identity = 82/175 (46.86%), Postives = 111/175 (63.43%), Query Frame = 1


Query: 1   VECLQTELEKLRKENEALKLMLKVVSIKNLVSQVDVCSRDHRHDQNDEGSRSERANLKV- 60
           VE LQ ELE+ RKENE L+ M++V+S K    Q +V        +N      E +N +V 
Sbjct: 11  VEALQYELERTRKENETLRFMVEVMSAKFSSLQENV-------QENKLFKVQESSNYEVY 70

Query: 61  -SPPALALALETSSTTQAYARTTFKDQALMVKDGYKWRKYGQKITKDNQSPRAYFKCS-S 120
            S     + +  +  +Q + RT  KD +L+V+DGY+WRKYGQK+TKDN SPRAYF+CS +
Sbjct: 71  DSIKRQRIEIPMAKPSQIFVRTESKDTSLIVRDGYQWRKYGQKVTKDNPSPRAYFRCSMA 130

Query: 121 PGCPVKKKVQRSLENKSMVIVTYDGHHNHNHNHENASPPPLSSSQRGSSSSPPLP 173
           PGCPVKKKVQR +E+KS+++ TY+G HNH+    +    P SSS R S SS   P
Sbjct: 131 PGCPVKKKVQRCVEDKSLLVATYEGEHNHDRTSGHFLYSPDSSSSRNSRSSISCP 178

[Close]

BLAST of Cucsa.162820 vs. TrEMBL
Match: V7CSB8_PHAVU (Uncharacterized protein OS=Phaseolus vulgaris GN=PHAVU_001G042100g PE=4 SV=1)

HSP 1 Score: 141.4 bits (355), Expect = 1.1e-30
Identity = 83/182 (45.60%), Postives = 113/182 (62.09%), Query Frame = 1


Query: 1   VECLQTELEKLRKENEALKLMLKVVSIKNLVSQVDVCSRDHRHDQNDEGSRSERANLK-- 60
           VE L++ELE++R+EN AL++ML+V+S K  V       + H  + N E  +   +N +  
Sbjct: 16  VEALKSELERVREENNALRVMLEVLSSKYTVL------KSHLQEINKEQHKGMSSNQRGS 75

Query: 61  VSPPAL------ALALETSSTT-QAYARTTFKDQALMVKDGYKWRKYGQKITKDNQSPRA 120
           V+ P         L   T+    Q + RT   D +L+VKDGY+WRKYGQK+TKDN SPRA
Sbjct: 76  VTEPITDANKRRRLEFPTAKKPLQIFVRTLPTDDSLIVKDGYQWRKYGQKVTKDNASPRA 135

Query: 121 YFKCS-SPGCPVKKKVQRSLENKSMVIVTYDGHHNHNHNHENASPPPLSSSQRGSSSSPP 173
           YF+CS +P CP KKKVQR L +KS+++ TYDG HNH   HE++S  P     RGSS +  
Sbjct: 136 YFRCSMAPICPAKKKVQRCLHDKSILVATYDGEHNHGALHESSSSTP-----RGSSVAKQ 186

[Close]

BLAST of Cucsa.162820 vs. TrEMBL
Match: A0A140H8P4_MANES (WRKY transcription factor 40 OS=Manihot esculenta PE=2 SV=1)

HSP 1 Score: 141.0 bits (354), Expect = 1.4e-30
Identity = 84/183 (45.90%), Postives = 112/183 (61.20%), Query Frame = 1


Query: 1   VECLQTELEKLRKENEALKLMLKVVSIKNLVSQVDVCSRDHRHDQNDEGSRSERANLKVS 60
           VE LQ ELE+ RKENE LK+M++V+S K    + +V          + GS S  +   VS
Sbjct: 13  VEALQDELERSRKENETLKVMVEVMSGKLSALRSNV---------QEIGSNSPLSG--VS 72

Query: 61  PPAL-------ALALETSSTTQAYARTTFKDQALMVKDGYKWRKYGQKITKDNQSPRAYF 120
           P  L        + +  S  +Q   +T  KD++L +KDGY+WRKYGQK+TKDN SPRAYF
Sbjct: 73  PYELYGLNKRPRIEIPMSKPSQILVKTDPKDKSLNLKDGYQWRKYGQKVTKDNPSPRAYF 132

Query: 121 KCS-SPGCPVKKKVQRSLENKSMVIVTYDGHHNHNHN----HENASPPPLSSSQRGSSSS 172
           +CS +P CPVKKKVQR  E+K+ ++ TY+G HNH+ N    H   SP  LS S R S+ S
Sbjct: 133 RCSMAPSCPVKKKVQRCAEDKTFLVATYEGEHNHDPNGSPGHSMYSPDSLSRSSRSSTVS 184

[Close]

BLAST of Cucsa.162820 vs. TrEMBL
Match: A0A140H8Q3_MANES (WRKY transcription factor 49 OS=Manihot esculenta PE=2 SV=1)

HSP 1 Score: 140.2 bits (352), Expect = 2.4e-30
Identity = 85/178 (47.75%), Postives = 112/178 (62.92%), Query Frame = 1


Query: 2   ECLQTELEKLRKENEALKLMLKVVSIKNLVSQVDVCSRD---HRHDQNDEGSRSERANLK 61
           E LQ +LE+ RKENEAL++ML+V+S K  V Q  V  +    H   +N   S S  +  +
Sbjct: 12  EALQEKLERSRKENEALRVMLEVMSSKLSVLQSRVQEKKFKVHEMGRNSPFSGSGASYNE 71

Query: 62  VSPPALALALET--SSTTQAYARTTFKDQALMVKDGYKWRKYGQKITKDNQSPRAYFKCS 121
           V  P     +E   +  +Q + RT  KD++ +VKDGY+WRKYGQK+TKDN +PRAYF+CS
Sbjct: 72  VYDPIKKQRIEIPMAKPSQFFFRTDPKDKSPIVKDGYQWRKYGQKVTKDNPAPRAYFRCS 131

Query: 122 -SPGCPVKKKVQRSLENKSMVIVTYDGHHNHNHNHENASPPP-LSSSQRGSSSSPPLP 173
            +PGCPVKKKVQR  E+KS ++ TY+G HNH+    N SP   L SS   S SS   P
Sbjct: 132 MAPGCPVKKKVQRCAEDKSFLVATYEGEHNHD---PNGSPGHILYSSPESSRSSISCP 186

[Close]

BLAST of Cucsa.162820 vs. TAIR10
Match: AT2G25000.1 (AT2G25000.1 WRKY DNA-binding protein 60)

HSP 1 Score: 114.0 bits (284), Expect = 9.2e-26
Identity = 70/168 (41.67%), Postives = 99/168 (58.93%), Query Frame = 1


Query: 4   LQTELEKLRKENEALKLML-----KVVSIKNLVSQVDVC-SRDHRHDQNDE--GSRSERA 63
           LQ E+ ++  EN+ L  ML     K  ++ NL+ ++    S +  + QN +  G R +  
Sbjct: 45  LQDEINRVNSENKKLTEMLARVCEKYYALNNLMEELQSRKSPESVNFQNKQLTGKRKQEL 104

Query: 64  NLKVSPPALALAL--------ETSSTTQAYARTTFKDQALMVKDGYKWRKYGQKITKDNQ 123
           +  VS P + L+L        + ++ + AY      D +L VKDGY+WRKYGQKIT+DN 
Sbjct: 105 DEFVSSP-IGLSLGPIENITNDKATVSTAYFAAEKSDTSLTVKDGYQWRKYGQKITRDNP 164

Query: 124 SPRAYFKCS-SPGCPVKKKVQRSLENKSMVIVTYDGHHNHNHNHENAS 155
           SPRAYF+CS SP C VKKKVQRS E+ S ++ TY+G HNH   H + S
Sbjct: 165 SPRAYFRCSFSPSCLVKKKVQRSAEDPSFLVATYEGTHNHTGPHASVS 211

[Close]

BLAST of Cucsa.162820 vs. TAIR10
Match: AT4G31800.1 (AT4G31800.1 WRKY DNA-binding protein 18)

HSP 1 Score: 109.4 bits (272), Expect = 2.3e-24
Identity = 60/167 (35.93%), Postives = 97/167 (58.08%), Query Frame = 1


Query: 2   ECLQTELEKLRKENEALKLMLKVVSIKNLVSQVDVCSRDHRHDQNDEGSRSERANLKVSP 61
           E  QT++   +++ +  + +   + + +  ++    + DH H       +++  + K   
Sbjct: 91  EIEQTDIPIKKRKQDPDEFLGFPIGLSSGKTENSSSNEDHHHHHQQHEQKNQLLSCK--- 150

Query: 62  PALALALETSSTTQAYARTTFKDQALMVKDGYKWRKYGQKITKDNQSPRAYFKCS-SPGC 121
             +  +   +  +  Y  T   D +L VKDG++WRKYGQK+T+DN SPRAYF+CS +P C
Sbjct: 151 RPVTDSFNKAKVSTVYVPTETSDTSLTVKDGFQWRKYGQKVTRDNPSPRAYFRCSFAPSC 210

Query: 122 PVKKKVQRSLENKSMVIVTYDGHHNHNHNHENASPPPLSSSQRGSSS 168
           PVKKKVQRS E+ S+++ TY+G   HNH   NAS    ++SQ GSS+
Sbjct: 211 PVKKKVQRSAEDPSLLVATYEG--THNHLGPNASEGD-ATSQGGSST 251

[Close]

BLAST of Cucsa.162820 vs. TAIR10
Match: AT1G80840.1 (AT1G80840.1 WRKY DNA-binding protein 40)

HSP 1 Score: 107.8 bits (268), Expect = 6.6e-24
Identity = 50/111 (45.05%), Postives = 76/111 (68.47%), Query Frame = 1


Query: 74  TQAYARTTFKDQALMVKDGYKWRKYGQKITKDNQSPRAYFKCS-SPGCPVKKKVQRSLEN 133
           ++ Y +T   D  L+VKDGY+WRKYGQK+T+DN SPRAYFKC+ +P C VKKKVQRS+E+
Sbjct: 130 SRVYYKTEASDTTLVVKDGYQWRKYGQKVTRDNPSPRAYFKCACAPSCSVKKKVQRSVED 189

Query: 134 KSMVIVTYDGHHNH--------------NHNHENASPPPLSSSQRGSSSSP 170
           +S+++ TY+G HNH              + +H  ++  P+++++R S + P
Sbjct: 190 QSVLVATYEGEHNHPMPSQIDSNNGLNRHISHGGSASTPVAANRRSSLTVP 240

[Close]

BLAST of Cucsa.162820 vs. TAIR10
Match: AT1G13960.1 (AT1G13960.1 WRKY DNA-binding protein 4)

HSP 1 Score: 94.7 bits (234), Expect = 5.8e-20
Identity = 43/126 (34.13%), Postives = 74/126 (58.73%), Query Frame = 1


Query: 44  DQNDEGSRSERANLKVSPPALALALETSSTTQAYARTTFKDQALMVKDGYKWRKYGQKIT 103
           D+N+   +     +++S PA A +  T +  +   +TT   +  ++ DGY+WRKYGQK+ 
Sbjct: 365 DENEPDPKRRSTEVRISEPAPAASHRTVTEPRIIVQTT--SEVDLLDDGYRWRKYGQKVV 424

Query: 104 KDNQSPRAYFKCSSPGCPVKKKVQRSLENKSMVIVTYDGHHNHNHNHENASPPPLSSSQR 163
           K N  PR+Y+KC++PGC V+K V+R+  +   V+ TY+G HNH+     +S    +++Q 
Sbjct: 425 KGNPYPRSYYKCTTPGCGVRKHVERAATDPKAVVTTYEGKHNHDLPAAKSSSHAAAAAQL 484

Query: 164 GSSSSP 170
              + P
Sbjct: 485 RPDNRP 488

HSP 2 Score: 81.6 bits (200), Expect = 5.0e-16
Identity = 47/99 (47.47%), Postives = 60/99 (60.61%), Query Frame = 1


Query: 52  SERANLKVSPPALALALETSSTTQAYARTTFKDQALMV----KDGYKWRKYGQKITKDNQ 111
           S +A +  S P L    ETS  T    R+    Q L V     DGY WRKYGQK  K ++
Sbjct: 191 SGQAQIPTSAP-LPAQRETSDVTIIEHRS---QQPLNVDKPADDGYNWRKYGQKQVKGSE 250

Query: 112 SPRAYFKCSSPGCPVKKKVQRSLENKSMVIVTYDGHHNH 147
            PR+Y+KC++PGCPVKKKV+RSL+ +   I+ Y G HNH
Sbjct: 251 FPRSYYKCTNPGCPVKKKVERSLDGQVTEII-YKGQHNH 284

[Close]

BLAST of Cucsa.162820 vs. TAIR10
Match: AT2G04880.1 (AT2G04880.1 zinc-dependent activator protein-1)

HSP 1 Score: 89.7 bits (221), Expect = 1.9e-18
Identity = 48/124 (38.71%), Postives = 73/124 (58.87%), Query Frame = 1


Query: 27  IKNLVSQVDVCSRDHRH-DQNDEGS--RSERANLKVSPPALALALETSSTTQAYARTTFK 86
           +K  +SQ    + D+ H D N   +  R +  N+++SP   +      S    + +T F 
Sbjct: 248 VKTDISQSSRITGDNTHKDYNSPTAKRRKKGGNIELSPVERST---NDSRIVVHTQTLFD 307

Query: 87  DQALMVKDGYKWRKYGQKITKDNQSPRAYFKCSSPGCPVKKKVQRSLENKSMVIVTYDGH 146
               +V DGY+WRKYGQK  K +  PR+Y++CSSPGCPVKK V+RS  +  ++I TY+G 
Sbjct: 308 ----IVNDGYRWRKYGQKSVKGSPYPRSYYRCSSPGCPVKKHVERSSHDTKLLITTYEGK 364

Query: 147 HNHN 148
           H+H+
Sbjct: 368 HDHD 364

HSP 2 Score: 67.4 bits (163), Expect = 9.8e-12
Identity = 38/95 (40.00%), Postives = 59/95 (62.11%), Query Frame = 1


Query: 52  SERANLKVSPPALALALETSSTTQAYARTTFKDQALMVKDGYKWRKYGQKITKDNQSPRA 111
           SE+A  KVS  + AL+L++ S       + F  + +M +DGY WRKYGQK+ K N+  R+
Sbjct: 79  SEKAP-KVSESSGALSLQSGSE----GNSPFIREKVM-EDGYNWRKYGQKLVKGNEFVRS 138

Query: 112 YFKCSSPGCPVKKKVQRSLENKSMVIVTYDGHHNH 147
           Y++C+ P C  KK+++RS   + +V   Y G H+H
Sbjct: 139 YYRCTHPNCKAKKQLERSAGGQ-VVDTVYFGEHDH 166

[Close]

BLAST of Cucsa.162820 vs. NCBI nr
Match: gi|449442146|ref|XP_004138843.1| (PREDICTED: probable WRKY transcription factor 60 [Cucumis sativus])

HSP 1 Score: 351.3 bits (900), Expect = 9.7e-94
Identity = 172/172 (100.00%), Postives = 172/172 (100.00%), Query Frame = 1


Query: 1   VECLQTELEKLRKENEALKLMLKVVSIKNLVSQVDVCSRDHRHDQNDEGSRSERANLKVS 60
           VECLQTELEKLRKENEALKLMLKVVSIKNLVSQVDVCSRDHRHDQNDEGSRSERANLKVS
Sbjct: 5   VECLQTELEKLRKENEALKLMLKVVSIKNLVSQVDVCSRDHRHDQNDEGSRSERANLKVS 64

Query: 61  PPALALALETSSTTQAYARTTFKDQALMVKDGYKWRKYGQKITKDNQSPRAYFKCSSPGC 120
           PPALALALETSSTTQAYARTTFKDQALMVKDGYKWRKYGQKITKDNQSPRAYFKCSSPGC
Sbjct: 65  PPALALALETSSTTQAYARTTFKDQALMVKDGYKWRKYGQKITKDNQSPRAYFKCSSPGC 124

Query: 121 PVKKKVQRSLENKSMVIVTYDGHHNHNHNHENASPPPLSSSQRGSSSSPPLP 173
           PVKKKVQRSLENKSMVIVTYDGHHNHNHNHENASPPPLSSSQRGSSSSPPLP
Sbjct: 125 PVKKKVQRSLENKSMVIVTYDGHHNHNHNHENASPPPLSSSQRGSSSSPPLP 176

[Close]

BLAST of Cucsa.162820 vs. NCBI nr
Match: gi|659088980|ref|XP_008445263.1| (PREDICTED: probable WRKY transcription factor 60 [Cucumis melo])

HSP 1 Score: 308.9 bits (790), Expect = 5.5e-81
Identity = 156/172 (90.70%), Postives = 161/172 (93.60%), Query Frame = 1


Query: 1   VECLQTELEKLRKENEALKLMLKVVSIKNLVSQVDVCSRDHRHDQNDEGSRSERANLKVS 60
           VECLQTELEKLRKENE LK MLKVVSIKNLVSQVDVCSRDH  DQNDEGSRSERA+L+VS
Sbjct: 5   VECLQTELEKLRKENEVLKFMLKVVSIKNLVSQVDVCSRDH--DQNDEGSRSERADLRVS 64

Query: 61  PPALALALETSSTTQAYARTTFKDQALMVKDGYKWRKYGQKITKDNQSPRAYFKCSSPGC 120
           PP     LETSSTTQAYA T+FKDQALMVKDGYKWRKYGQKITKDNQSPRAYFKCSSPGC
Sbjct: 65  PPT----LETSSTTQAYATTSFKDQALMVKDGYKWRKYGQKITKDNQSPRAYFKCSSPGC 124

Query: 121 PVKKKVQRSLENKSMVIVTYDGHHNHNHNHENASPPPLSSSQRGSSSSPPLP 173
           PVKK+VQRSLENKSMVIVTYDGHHNHNHNHENASPPPLS+SQRG SSSPPLP
Sbjct: 125 PVKKEVQRSLENKSMVIVTYDGHHNHNHNHENASPPPLSASQRG-SSSPPLP 169

[Close]

BLAST of Cucsa.162820 vs. NCBI nr
Match: gi|1009133681|ref|XP_015884033.1| (PREDICTED: probable WRKY transcription factor 40 [Ziziphus jujuba])

HSP 1 Score: 150.2 bits (378), Expect = 3.3e-33
Identity = 79/169 (46.75%), Postives = 116/169 (68.64%), Query Frame = 1


Query: 2   ECLQTELEKLRKENEALKLMLKVVSIKNLVSQVDVCSRDHRH---DQNDEGSRSERANLK 61
           E LQ +LE +RKENE+L+ ML+V++ K  + +  +  R+  +   D N +   ++RA   
Sbjct: 12  EALQEDLECMRKENESLRFMLEVMTRKCNIIESHLRQRNLEYMGIDLNHQFEANKRARTS 71

Query: 62  VSPPALALALETSSTTQAYARTTFKDQALMVKDGYKWRKYGQKITKDNQSPRAYFKCS-S 121
           ++    +LA   S T+Q   RT  KD  L+VKDGY+WRKYGQK+TKDN SPRAYF+CS +
Sbjct: 72  INE--FSLATNKSQTSQFLVRTNSKDSTLVVKDGYQWRKYGQKVTKDNPSPRAYFRCSMA 131

Query: 122 PGCPVKKKVQRSLENKSMVIVTYDGHHNHNHNHENAS-PPPLSSSQRGS 166
           PGCPVKKKVQRS ++KS++I TY+G HNH+ +  +A   P +++S +G+
Sbjct: 132 PGCPVKKKVQRSKDDKSVLIATYEGQHNHDSHDSHAHFSPSINTSAKGA 178

[Close]

BLAST of Cucsa.162820 vs. NCBI nr
Match: gi|802626582|ref|XP_012076437.1| (PREDICTED: probable WRKY transcription factor 40 [Jatropha curcas])

HSP 1 Score: 147.9 bits (372), Expect = 1.6e-32
Identity = 82/175 (46.86%), Postives = 111/175 (63.43%), Query Frame = 1


Query: 1   VECLQTELEKLRKENEALKLMLKVVSIKNLVSQVDVCSRDHRHDQNDEGSRSERANLKV- 60
           VE LQ ELE+ RKENE L+ M++V+S K    Q +V        +N      E +N +V 
Sbjct: 11  VEALQYELERTRKENETLRFMVEVMSAKFSSLQENV-------QENKLFKVQESSNYEVY 70

Query: 61  -SPPALALALETSSTTQAYARTTFKDQALMVKDGYKWRKYGQKITKDNQSPRAYFKCS-S 120
            S     + +  +  +Q + RT  KD +L+V+DGY+WRKYGQK+TKDN SPRAYF+CS +
Sbjct: 71  DSIKRQRIEIPMAKPSQIFVRTESKDTSLIVRDGYQWRKYGQKVTKDNPSPRAYFRCSMA 130

Query: 121 PGCPVKKKVQRSLENKSMVIVTYDGHHNHNHNHENASPPPLSSSQRGSSSSPPLP 173
           PGCPVKKKVQR +E+KS+++ TY+G HNH+    +    P SSS R S SS   P
Sbjct: 131 PGCPVKKKVQRCVEDKSLLVATYEGEHNHDRTSGHFLYSPDSSSSRNSRSSISCP 178

[Close]

BLAST of Cucsa.162820 vs. NCBI nr
Match: gi|1000974347|ref|XP_015572479.1| (PREDICTED: probable WRKY transcription factor 40 [Ricinus communis])

HSP 1 Score: 141.7 bits (356), Expect = 1.2e-30
Identity = 79/175 (45.14%), Postives = 109/175 (62.29%), Query Frame = 1


Query: 1   VECLQTELEKLRKENEALKLMLKVVSIKNLVSQVDVCSRDHRHDQNDEGSRSERANLKVS 60
           +E LQ ELE+ +KENE L+ ML+V+S K    Q +   +  +   +        +N +  
Sbjct: 11  MESLQDELERTQKENETLRFMLEVMSRKFSTLQANFQEKKVQETPSSSCYEVYESNKR-- 70

Query: 61  PPALALALETSSTTQAYARTTFKDQALMVKDGYKWRKYGQKITKDNQSPRAYFKCS-SPG 120
            P + + L  S  +Q + RT  KD++L+V+DGY+WRKYGQK+TKDN SPRAYF+CS +PG
Sbjct: 71  -PRIEIPL--SKPSQIFVRTDSKDKSLIVRDGYQWRKYGQKVTKDNPSPRAYFRCSMAPG 130

Query: 121 CPVKKKVQRSLENKSMVIVTYDGHHNHNHNHENA----SPPPLSSSQRGSSSSPP 171
           CPVKKKVQR  E+KS+++ TY+G HNH  N   A    SP   S S R + S  P
Sbjct: 131 CPVKKKVQRCAEDKSILVATYEGEHNHEPNDSPAQSVYSPDSSSRSSRTTISFQP 180

The following BLAST results are available for this feature:

BLAST of Cucsa.162820 vs. Swiss-Prot


Match Name	E-value	Identity	Description
WRK60_ARATH	1.6e-24	41.67	Probable WRKY transcription factor 60 OS=Arabidopsis thaliana GN=WRKY60 PE=1 SV=...	[more]
WRK18_ARATH	4.0e-23	35.93	WRKY transcription factor 18 OS=Arabidopsis thaliana GN=WRKY18 PE=1 SV=2	[more]
WRK40_ARATH	1.2e-22	45.05	Probable WRKY transcription factor 40 OS=Arabidopsis thaliana GN=WRKY40 PE=1 SV=...	[more]
WRKY4_ARATH	1.0e-18	34.13	Probable WRKY transcription factor 4 OS=Arabidopsis thaliana GN=WRKY4 PE=1 SV=2	[more]
WRK46_HORVU	8.6e-18	45.45	WRKY transcription factor SUSIBA2 OS=Hordeum vulgare GN=WRKY46 PE=1 SV=1	[more]

BLAST of Cucsa.162820 vs. TrEMBL


Match Name	E-value	Identity	Description
A0A0A0LMJ1_CUCSA	6.7e-94	100.00	Uncharacterized protein OS=Cucumis sativus GN=Csa_2G382660 PE=4 SV=1	[more]
S5CS62_JATCU	1.1e-32	46.86	Uncharacterized protein OS=Jatropha curcas GN=WRKY29 PE=4 SV=1	[more]
V7CSB8_PHAVU	1.1e-30	45.60	Uncharacterized protein OS=Phaseolus vulgaris GN=PHAVU_001G042100g PE=4 SV=1	[more]
A0A140H8P4_MANES	1.4e-30	45.90	WRKY transcription factor 40 OS=Manihot esculenta PE=2 SV=1	[more]
A0A140H8Q3_MANES	2.4e-30	47.75	WRKY transcription factor 49 OS=Manihot esculenta PE=2 SV=1	[more]

BLAST of Cucsa.162820 vs. TAIR10


Match Name	E-value	Identity	Description
AT2G25000.1	9.2e-26	41.67	WRKY DNA-binding protein 60	[more]
AT4G31800.1	2.3e-24	35.93	WRKY DNA-binding protein 18	[more]
AT1G80840.1	6.6e-24	45.05	WRKY DNA-binding protein 40	[more]
AT1G13960.1	5.8e-20	34.13	WRKY DNA-binding protein 4	[more]
AT2G04880.1	1.9e-18	38.71	zinc-dependent activator protein-1	[more]

BLAST of Cucsa.162820 vs. NCBI nr


Match Name	E-value	Identity	Description
gi\|449442146\|ref\|XP_004138843.1\|	9.7e-94	100.00	PREDICTED: probable WRKY transcription factor 60 [Cucumis sativus]	[more]
gi\|659088980\|ref\|XP_008445263.1\|	5.5e-81	90.70	PREDICTED: probable WRKY transcription factor 60 [Cucumis melo]	[more]
gi\|1009133681\|ref\|XP_015884033.1\|	3.3e-33	46.75	PREDICTED: probable WRKY transcription factor 40 [Ziziphus jujuba]	[more]
gi\|802626582\|ref\|XP_012076437.1\|	1.6e-32	46.86	PREDICTED: probable WRKY transcription factor 40 [Jatropha curcas]	[more]
gi\|1000974347\|ref\|XP_015572479.1\|	1.2e-30	45.14	PREDICTED: probable WRKY transcription factor 40 [Ricinus communis]	[more]

Annotated Terms

The following terms have been associated with this gene:

Vocabulary: **INTERPRO**
Term	Definition
IPR003657	WRKY_dom

Vocabulary: **Molecular Function**
Term	Definition
GO:0003700	transcription factor activity, sequence-specific DNA binding
GO:0043565	sequence-specific DNA binding

Vocabulary: **Biological Process**
Term	Definition
GO:0006355	regulation of transcription, DNA-templated

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006355	regulation of transcription, DNA-templated
cellular_component	GO:0005615	extracellular space
cellular_component	GO:0005667	transcription factor complex
molecular_function	GO:0043565	sequence-specific DNA binding
molecular_function	GO:0003700	transcription factor activity, sequence-specific DNA binding

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Cucsa.162820.1	Cucsa.162820.1	mRNA

InterPro

Analysis Name: InterPro Annotations of cucumber (Gy14)
Date Performed: 2017-01-17


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR003657	WRKY domain	GENE3D	G3DSA:2.20.25.80		coord: 81..148 score: 9.5
IPR003657	WRKY domain	PFAM	PF03106	WRKY	coord: 90..146 score: 6.9
IPR003657	WRKY domain	SMART	SM00774	WRKY_cls	coord: 89..148 score: 1.3
IPR003657	WRKY domain	PROFILE	PS50811	WRKY	coord: 84..149 score: 25
IPR003657	WRKY domain	unknown	SSF118290	WRKY DNA-binding domain	coord: 85..148 score: 5.36
None	No IPR available	unknown	Coil	Coil	coord: 1..28 scor
None	No IPR available	PANTHER	PTHR31429	FAMILY NOT NAMED	coord: 73..168 score: 8.5
None	No IPR available	PANTHER	PTHR31429:SF3	WRKY TRANSCRIPTION FACTOR 40-RELATED	coord: 73..168 score: 8.5

Synteny

The following gene(s) are orthologous to this gene:


Gene	Orthologue	Organism	Block
Cucsa.162820	Lsi08G012450	Bottle gourd (USVL1VR-Ls)	cgylsiB267
Cucsa.162820	MELO3C011296.2	Melon (DHL92) v3.6.1	cgymedB273
Cucsa.162820	Carg02843	Silver-seed gourd	carcgyB0709

The following gene(s) are paralogous to this gene:

None

The following block(s) are covering this gene:


Gene	Organism	Block
Cucsa.162820	Silver-seed gourd	carcgyB0171
Cucsa.162820	Silver-seed gourd	carcgyB1091
Cucsa.162820	Cucumber (Chinese Long) v3	cgycucB247
Cucsa.162820	Cucumber (Chinese Long) v3	cgycucB255
Cucsa.162820	Watermelon (97103) v2	cgywmbB280
Cucsa.162820	Watermelon (97103) v2	cgywmbB282
Cucsa.162820	Wax gourd	cgywgoB313
Cucsa.162820	Wax gourd	cgywgoB322
Cucsa.162820	Cucumber (Gy14) v1	cgycgyB099
Cucsa.162820	Cucurbita maxima (Rimu)	cgycmaB0444
Cucsa.162820	Cucurbita maxima (Rimu)	cgycmaB0447
Cucsa.162820	Cucurbita maxima (Rimu)	cgycmaB0462
Cucsa.162820	Cucurbita moschata (Rifu)	cgycmoB0448
Cucsa.162820	Cucurbita moschata (Rifu)	cgycmoB0463
Cucsa.162820	Wild cucumber (PI 183967)	cgycpiB239
Cucsa.162820	Wild cucumber (PI 183967)	cgycpiB246
Cucsa.162820	Cucumber (Chinese Long) v2	cgycuB233
Cucsa.162820	Cucumber (Chinese Long) v2	cgycuB240
Cucsa.162820	Melon (DHL92) v3.5.1	cgymeB276
Cucsa.162820	Melon (DHL92) v3.5.1	cgymeB283
Cucsa.162820	Watermelon (Charleston Gray)	cgywcgB279
Cucsa.162820	Watermelon (Charleston Gray)	cgywcgB282
Cucsa.162820	Watermelon (97103) v1	cgywmB289
Cucsa.162820	Watermelon (97103) v1	cgywmB293
Cucsa.162820	Cucurbita pepo (Zucchini)	cgycpeB0430
Cucsa.162820	Cucurbita pepo (Zucchini)	cgycpeB0442
Cucsa.162820	Cucurbita pepo (Zucchini)	cgycpeB0444
Cucsa.162820	Bottle gourd (USVL1VR-Ls)	cgylsiB264
Cucsa.162820	Melon (DHL92) v3.6.1	cgymedB279