Cucsa.161360 (gene) Cucumber (Gy14) v1

BLAST of Cucsa.161360 vs. Swiss-Prot
Match: PTR6_ARATH (Protein NRT1/ PTR FAMILY 1.2 OS=Arabidopsis thaliana GN=NPF1.2 PE=1 SV=1)

HSP 1 Score: 570.1 bits (1468), Expect = 2.8e-161
Identity = 287/547 (52.47%), Postives = 388/547 (70.93%), Query Frame = 1

Query: 25  KGGFRTLPFIIANGALEKLASQGLSPSMILYLTKVYGMKSAHASNVIFLWSAASNFTPII 84
           KGG  T+PFIIAN A EK+AS GL P+MI+YL + Y    A  +NV+F+WSAASNFTP++
Sbjct: 22  KGGIITMPFIIANEAFEKVASYGLLPNMIMYLIRDYRFGVAKGTNVLFMWSAASNFTPLL 81

Query: 85  CAFLADSYFGRFPMIAAGSIFSFLGMFVLWLTAMIPQARPF-CDEISG--HCDAPSTSQL 144
            AFL+DSY GRF  I+  S+ SFLGM +LWLTAM+PQ +P  CD  +   HC + + SQL
Sbjct: 82  GAFLSDSYLGRFLTISIASLSSFLGMVLLWLTAMLPQVKPSPCDPTAAGSHCGSSTASQL 141

Query: 145 LLLYSSYAIMSVGSGCLQASYLAFGADQLYRK-NKSNSGILDSYFNVCYISAALGTLVGM 204
            LLYS++A++S+GSG ++   LAFGADQL  K N  N  +L+S+F   Y S+A+  L+  
Sbjct: 142 ALLYSAFALISIGSGGIRPCSLAFGADQLDNKENPKNERVLESFFGWYYASSAVAVLIAF 201

Query: 205 SCIVYIQDRMGWGMGFGVPVALMFLATITFLSASPLYLKSKPSKSWCAGLVQVVFAAYKK 264
           + IVYIQ+ +GW +GFGVP  LM +A + F+ ASPLY+    +KS   GL Q + AAYKK
Sbjct: 202 TGIVYIQEHLGWKIGFGVPAVLMLIAALLFILASPLYVTRGVTKSLFTGLAQAIVAAYKK 261

Query: 265 RHTQIPFVGTS-EMYHHENESPCTLPSNKLRFLNKACIIRNSKEELTLDGKASNPWSLCT 324
           R   +P    S + Y+H  +S    PS KLRFLNKAC+I N +EE+  DG A NPW LCT
Sbjct: 262 RKLSLPDHHDSFDCYYHMKDSEIKAPSQKLRFLNKACLISNREEEIGSDGFALNPWRLCT 321

Query: 325 VEQVENLKALIRIIPLWSTGILVSASLSQS-FYVLQVASMNRHLT---SSFEVPAGSFSA 384
            ++VE LKALI++IP+WSTGI++S + SQS F +LQ  SM+R L+   SSF+VPAGSF  
Sbjct: 322 TDKVEELKALIKVIPIWSTGIMMSINTSQSSFQLLQATSMDRRLSRHGSSFQVPAGSFGM 381

Query: 385 IIVVSLIIWIALYDRLILPLASKCRGKPTRLCAKTRMGMGILCCCLSLAVSAIVEGDRRA 444
             +++L +W+ LYDR ++PLASK RG+P RL  K RMG+G+    L++A+SA+VE  RR 
Sbjct: 382 FTIIALALWVILYDRAVIPLASKIRGRPFRLSVKLRMGLGLFMSFLAMAISAMVESFRRK 441

Query: 445 LAIKEGFSDNPNAVVSMSAFWTLPRYILFGIAEALNAIGQIEFFYNELPKAMSSVATSLL 504
            AI +G+++N NAVV +SA W +P+Y+L G+AEAL AIGQ EFFY E PK+MSS+A SL 
Sbjct: 442 KAISQGYANNSNAVVDISAMWLVPQYVLHGLAEALTAIGQTEFFYTEFPKSMSSIAASLF 501

Query: 505 GLNSSIGNLAASFIMTTVDNLSKSVGVKSWVSSNINEGHSDYYYWLLSGLLFANFLYYLA 563
           GL  ++ +L AS ++  V+ L+   G +SWVS NIN+GH +YYYW+L+ + F N +YY+ 
Sbjct: 502 GLGMAVASLLASVVLNAVNELTSRNGKESWVSDNINKGHYNYYYWVLAIMSFINVIYYVI 561

BLAST of Cucsa.161360 vs. Swiss-Prot
Match: PTR32_ARATH (Protein NRT1/ PTR FAMILY 1.1 OS=Arabidopsis thaliana GN=NPF1.1 PE=1 SV=2)

HSP 1 Score: 541.6 bits (1394), Expect = 1.1e-152
Identity = 281/549 (51.18%), Postives = 377/549 (68.67%), Query Frame = 1

Query: 25  KGGFRTLPFIIANGALEKLASQGLSPSMILYLTKVYGMKSAHASNVIFLWSAASNFTPII 84
           KGG  T+PFIIAN   EK+AS GL  +MILYL   Y +       V+F+W AA+NF P++
Sbjct: 24  KGGLLTMPFIIANEGFEKVASYGLLQNMILYLMSDYRLGLVKGQTVLFMWVAATNFMPLV 83

Query: 85  CAFLADSYFGRFPMIAAGSIFSFLGMFVLWLTAMIPQARPF-CDEISG-HCDAPSTSQLL 144
            AFL+DSY GRF  I   S+ S LGM VLWLTAM+PQ +P  C   +G +C + ++SQL 
Sbjct: 84  GAFLSDSYLGRFLTIVIASLSSLLGMVVLWLTAMLPQVKPSPCVATAGTNCSSATSSQLA 143

Query: 145 LLYSSYAIMSVGSGCLQASYLAFGADQLYRK-NKSNSGILDSYFNVCYISAALGTLVGMS 204
           LLY+++A++S+GSG ++   LAFGADQL  K N  N  +L+S+F   Y S+++  L+  +
Sbjct: 144 LLYTAFALISIGSGGIRPCSLAFGADQLDNKENPKNERVLESFFGWYYASSSVAVLIAFT 203

Query: 205 CIVYIQDRMGWGMGFGVPVALMFLATITFLSASPLYLKSKPSKSWCAGLVQVVFAAYKKR 264
            IVYIQD +GW +GFG+P  LM LA   F+ ASPLY+K   SKS   GL QVV AAY KR
Sbjct: 204 VIVYIQDHLGWKIGFGIPAILMLLAGFLFVFASPLYVKRDVSKSLFTGLAQVVAAAYVKR 263

Query: 265 HTQIPFVGTS-EMYHHENESPCTLPSNKLRFLNKACIIRNSKEELTLDGKASNPWSLCTV 324
           +  +P    S + Y+   +S    PS+KLRFLNKAC I N  E+L  DG A N W LCT 
Sbjct: 264 NLTLPDHHDSRDCYYRLKDSELKAPSDKLRFLNKACAISNRDEDLGSDGLALNQWRLCTT 323

Query: 325 EQVENLKALIRIIPLWSTGILVSASLSQ-SFYVLQVASMNRHLT--SSFEVPAGSFSAII 384
           +QVE LKAL+++IP+WSTGI++S ++SQ SF +LQ  SM+R L+  S+F++PAGSF    
Sbjct: 324 DQVEKLKALVKVIPVWSTGIMMSINVSQNSFQLLQAKSMDRRLSSNSTFQIPAGSFGMFT 383

Query: 385 VVSLIIWIALYDRLILPLASKCRGKPTRLCAKTRMGMGILCCCLSLAVSAIVEGDRRALA 444
           +++LI W+ LYDR ILPLASK RG+P R+  K RMG+G+    L++AVSA VE  RR  A
Sbjct: 384 IIALISWVVLYDRAILPLASKIRGRPVRVNVKIRMGLGLFISFLAMAVSATVEHYRRKTA 443

Query: 445 IKEGFSDNPNAVVSMSAFWTLPRYILFGIAEALNAIGQIEFFYNELPKAMSSVATSLLGL 504
           I +G +++ N+ VS+SA W +P+Y+L G+AEAL  IGQ EFFY E PK+MSS+A SL GL
Sbjct: 444 ISQGLANDANSTVSISAMWLVPQYVLHGLAEALTGIGQTEFFYTEFPKSMSSIAASLFGL 503

Query: 505 NSSIGNLAASFIMTTVDNLSKSVGVKSWVSSNINEGHSDYYYWLLSGLLFANFLYYLACS 564
             ++ N+ AS I+  V N SK  G  SW+  NIN+GH DYYYW+L+ L F N +YY+ CS
Sbjct: 504 GMAVANILASVILNAVKNSSKQ-GNVSWIEDNINKGHYDYYYWVLAILSFVNVIYYVVCS 563

Query: 565 KSYGPSNEE 567
            SYGP+ ++
Sbjct: 564 WSYGPTVDQ 571

BLAST of Cucsa.161360 vs. Swiss-Prot
Match: PTR21_ARATH (Protein NRT1/ PTR FAMILY 2.13 OS=Arabidopsis thaliana GN=NPF2.13 PE=1 SV=1)

HSP 1 Score: 354.0 bits (907), Expect = 3.2e-96
Identity = 192/550 (34.91%), Postives = 317/550 (57.64%), Query Frame = 1

Query: 22  DNRKGGFRTLPFIIANGALEKLASQGLSPSMILYLTKVYGMKSAHASNVIFLWSAASNFT 81
           + + GG+R + FI+ N  LE+L S GL  + ++YLTKV+ ++   A+NVI +WS  +N T
Sbjct: 49  EKKPGGWRAVSFILGNETLERLGSIGLLANFMVYLTKVFHLEQVDAANVINIWSGFTNLT 108

Query: 82  PIICAFLADSYFGRFPMIAAGSIFSFLGMFVLWLTAMIPQARPFC----DEISGHCDAPS 141
           P++ A+++D+Y GRF  IA  S  + LG+  + LTA  PQ  P      D +S  C  P+
Sbjct: 109 PLVGAYISDTYVGRFKTIAFASFATLLGLITITLTASFPQLHPASCNSQDPLS--CGGPN 168

Query: 142 TSQLLLLYSSYAIMSVGSGCLQASYLAFGADQLYRKNKSNSGILDSYFNVCYISAALGTL 201
             Q+ +L      +SVGSG ++   + FG DQ  ++ +     + S+FN  Y++  +  +
Sbjct: 169 KLQIGVLLLGLCFLSVGSGGIRPCSIPFGVDQFDQRTEEGVKGVASFFNWYYMTFTVVLI 228

Query: 202 VGMSCIVYIQDRMGWGMGFGVPVALMFLATITFLSASPLYLKSKPSKSWCAGLVQVVFAA 261
           +  + +VYIQD++ W +GF +P  LM LA + F +    Y+  KP  S  +G+ QV+ AA
Sbjct: 229 ITQTVVVYIQDQVSWIIGFSIPTGLMALAVVMFFAGMKRYVYVKPEGSIFSGIAQVIVAA 288

Query: 262 YKKRHTQIPFVGTSEMYHHENESPCTL-----PSNKLRFLNKACIIRNSKEELTLDGKAS 321
            KKR  ++P      + +++     ++      SN+ R L+KA ++     +LT +G  +
Sbjct: 289 RKKRKLKLPAEDDGTVTYYDPAIKSSVLSKLHRSNQFRCLDKAAVVIEG--DLTPEGPPA 348

Query: 322 NPWSLCTVEQVENLKALIRIIPLWSTGILVSASLSQ--SFYVLQVASMNRHLTSSFEVPA 381
           + W LC+V++VE +K LIRI+P+WS GI+  A+++   +F V Q   M+R+L   FE+PA
Sbjct: 349 DKWRLCSVQEVEEVKCLIRIVPIWSAGIISLAAMTTQGTFTVSQALKMDRNLGPKFEIPA 408

Query: 382 GSFSAIIVVSLIIWIALYDRLILPLASKCRGKPTRLCAKTRMGMGILCCCLSLAVSAIVE 441
           GS S I ++++ I++  YDR+ +P   +  G  + +    R+G GI+    S+ V+ IVE
Sbjct: 409 GSLSVISLLTIGIFLPFYDRVFVPFMRRITGHKSGITLLQRIGTGIVFAIFSMIVAGIVE 468

Query: 442 GDRRALAIKEGFSDNPNAVVSMSAFWTLPRYILFGIAEALNAIGQIEFFYNELPKAMSSV 501
             RR  +I  G   +P  +  MS FW  P+ IL G+ EA N IGQIEFF ++ P+ M S+
Sbjct: 469 RMRRIRSINAG---DPTGMTPMSVFWLSPQLILMGLCEAFNIIGQIEFFNSQFPEHMRSI 528

Query: 502 ATSLLGLNSSIGNLAASFIMTTVDNLSKSVGVKSWVSSNINEGHSDYYYWLLSGLLFANF 561
           A SL  L+ +  +  +SF++T V   S       W++ N+N G  DY+Y+L++ L   N 
Sbjct: 529 ANSLFSLSFAGSSYLSSFLVTVVHKFSGGHDRPDWLNKNLNAGKLDYFYYLIAVLGVVNL 588

BLAST of Cucsa.161360 vs. Swiss-Prot
Match: PTR44_ARATH (Protein NRT1/ PTR FAMILY 2.10 OS=Arabidopsis thaliana GN=NPF2.10 PE=1 SV=3)

HSP 1 Score: 354.0 bits (907), Expect = 3.2e-96
Identity = 194/553 (35.08%), Postives = 315/553 (56.96%), Query Frame = 1

Query: 27  GFRTLPFIIANGALEKLASQGLSPSMILYLTKVYGMKSAHASNVIFLWSAASNFTPIICA 86
           G++ +PFII N   EKL   G   ++++YLT V+ +KS  A+ +I  +S   NF   I A
Sbjct: 64  GWKVMPFIIGNETFEKLGIIGTLSNLLVYLTSVFNLKSYTAATIINAFSGTINFGTFIAA 123

Query: 87  FLADSYFGRFPMIAAGSIFSFLGMFVLWLTAMIPQARPFCDEISGHCDAPSTSQLLLLYS 146
           FL D+YFGR+  ++   I  FLG FV+ LTA IP   P        C+ PS  Q+L L  
Sbjct: 124 FLCDTYFGRYKTLSVAVIACFLGSFVILLTAAIPSLHPVACGNKISCEGPSVGQILFLLM 183

Query: 147 SYAIMSVGSGCLQASYLAFGADQLYRKNKSNSGILDSYFNVCYISAALGTLVGMSCIVYI 206
               + VG+G ++   LAFGADQ   K++S    ++S+FN  + +     ++ ++ +VYI
Sbjct: 184 GLGFLVVGAGGIRPCNLAFGADQFNPKSESGKKGINSFFNWYFFTFTFAQIISLTAVVYI 243

Query: 207 QDRMGWGMGFGVPVALMFLATITFLSASPLYLKSKPSKSWCAGLVQVVFAAYKKRHTQIP 266
           Q  + W +G  +PVALMFLA + F +   LY+K K S S  AG+ +V+ AA KKR  +  
Sbjct: 244 QSNVSWTIGLIIPVALMFLACVIFFAGDRLYVKVKASGSPLAGIARVIAAAIKKRGLKPV 303

Query: 267 FVGTSEMYHH--ENESPCTLP-SNKLRFLNKACIIRNSKEELTLDGKASNPWSLCTVEQV 326
                 +Y+H   N +  TL  +++ RFL+KA I+   +E+L  DG AS+PW LCT++QV
Sbjct: 304 KQPWVNLYNHIPSNYANTTLKYTDQFRFLDKAAIM-TPEEKLNSDGTASDPWKLCTLQQV 363

Query: 327 ENLKALIRIIPLW--STGILVSASLSQSFYVLQVASMNRHLTS-SFEVPAGSFSAIIVVS 386
           E +K ++R+IP+W  ST   ++ ++  ++ V Q    +R L S  F +PA ++   ++  
Sbjct: 364 EEVKCIVRVIPIWFASTIYYLAITIQMTYPVFQALQSDRRLGSGGFRIPAATYVVFLMTG 423

Query: 387 LIIWIALYDRLILPLASKCRGKPTRLCAKTRMGMGILCCCLSLAVSAIVEGDRRALAIKE 446
           + ++I  YDR+++P   +  G  T +    R+G G     +SL VS  +E  RR  A+ +
Sbjct: 424 MTVFIIFYDRVLVPSLRRVTGLETGISLLQRIGAGFTFAIMSLLVSGFIEERRRNFALTK 483

Query: 447 ---GFSDNPNAVVSMSAFWTLPRYILFGIAEALNAIGQIEFFYNELPKAMSSVATSLLGL 506
              G +     + SMSA W +P+  L GIAEA  AIGQ+EF+Y + P+ M S A S+  +
Sbjct: 484 PTLGMAPRTGEISSMSALWLIPQLTLAGIAEAFAAIGQMEFYYKQFPENMKSFAGSIFYV 543

Query: 507 NSSIGNLAASFIMTTVDNLSKSVGVKSWVSSNINEGHSDYYYWLLSGLLFANFLYYLACS 566
            + + +  ASF+++TV   +      +W++ ++N+   DY+Y++L+GL+  N  Y+L  +
Sbjct: 544 GAGVSSYLASFLISTVHRTTAHSPSGNWLAEDLNKAKLDYFYFMLTGLMVVNMAYFLLMA 603

Query: 567 K--SYGPSNEESV 569
           +   Y   N+E +
Sbjct: 604 RWYRYKGGNDEDI 615

BLAST of Cucsa.161360 vs. Swiss-Prot
Match: PTR18_ARATH (Protein NRT1/ PTR FAMILY 3.1 OS=Arabidopsis thaliana GN=NPF3.1 PE=2 SV=1)

HSP 1 Score: 353.2 bits (905), Expect = 5.4e-96
Identity = 201/556 (36.15%), Postives = 322/556 (57.91%), Query Frame = 1

Query: 14  EEPLLSDTDNR-KGGFRTLPFIIANGALEKLASQGLSPSMILYLTKVYGMKSAHASNVIF 73
           EE  L    NR KGG  T+PFI AN   EKLA  G   +MI YLT    +    A+N + 
Sbjct: 12  EEKQLHGRPNRPKGGLITMPFIFANEICEKLAVVGFHANMISYLTTQLHLPLTKAANTLT 71

Query: 74  LWSAASNFTPIICAFLADSYFGRFPMIAAGSIFSFLGMFVLWLTAMIPQARPFCDEISGH 133
            ++  S+ TP++ AF+ADS+ GRF  I   SI   +GM +L ++A+IP  RP   +    
Sbjct: 72  NFAGTSSLTPLLGAFIADSFAGRFWTITFASIIYQIGMTLLTISAIIPTLRPPPCKGEEV 131

Query: 134 CDAPSTSQLLLLYSSYAIMSVGSGCLQASYLAFGADQLYRKNKSNSGILDSYFNVCYISA 193
           C    T+QL +LY +  + ++GSG ++   +AFGADQ    + + +    +YFN  Y   
Sbjct: 132 CVVADTAQLSILYVALLLGALGSGGIRPCVVAFGADQFDESDPNQTTKTWNYFNWYYFCM 191

Query: 194 ALGTLVGMSCIVYIQDRMGWGMGFGVPVALMFLATITFLSASPLYLKSKPSKSWCAGLVQ 253
               L+ ++ +V+IQD +GWG+G G+P   MFL+ I F+    LY    P+ S    L+Q
Sbjct: 192 GAAVLLAVTVLVWIQDNVGWGLGLGIPTVAMFLSVIAFVGGFQLYRHLVPAGSPFTRLIQ 251

Query: 254 VVFAAYKKRHTQIPFVGTSEMYHHENESPCTL-----PSNKLRFLNKACIIRNSKEELTL 313
           V  AA++KR  ++    +   ++ E ++P +L      +  + FL+KA I+  ++E+   
Sbjct: 252 VGVAAFRKRKLRMVSDPSLLYFNDEIDAPISLGGKLTHTKHMSFLDKAAIV--TEEDNLK 311

Query: 314 DGKASNPWSLCTVEQVENLKALIRIIPLWSTGILVSASLSQ--SFYVLQVASMNRHLTSS 373
            G+  N W L TV +VE LK++IR+ P+ ++GIL+  + +Q  +F + Q  +MNRHLT+S
Sbjct: 312 PGQIPNHWRLSTVHRVEELKSVIRMGPIGASGILLITAYAQQGTFSLQQAKTMNRHLTNS 371

Query: 374 FEVPAGSFSAIIVVSLIIWIALYDRLILPLASKCRGKPTRLCAKTRMGMGILCCCLSLAV 433
           F++PAGS S    V+++  I  YDR+ + +A K  G    +    RMG+G +   ++  V
Sbjct: 372 FQIPAGSMSVFTTVAMLTTIIFYDRVFVKVARKFTGLERGITFLHRMGIGFVISIIATLV 431

Query: 434 SAIVEGDRRALAIKEGFSDNPNAVVSMSAFWTLPRYILFGIAEALNAIGQIEFFYNELPK 493
           +  VE  R+++AI+ G  D P+ +V +S  W +P+Y L G+AEA  +IG +EFFY++ P+
Sbjct: 432 AGFVEVKRKSVAIEHGLLDKPHTIVPISFLWLIPQYGLHGVAEAFMSIGHLEFFYDQAPE 491

Query: 494 AMSSVATSLLGLNSSIGNLAASFIMTTVDNLSKSVGVKSWV-SSNINEGHSDYYYWLLSG 553
           +M S AT+L  +  SIGN  ++ ++T V   S      +W+  +N+N G  +Y+YWL++ 
Sbjct: 492 SMRSTATALFWMAISIGNYVSTLLVTLVHKFSAKPDGSNWLPDNNLNRGRLEYFYWLITV 551

Query: 554 LLFANFLYYLACSKSY 561
           L   N +YYL C+K Y
Sbjct: 552 LQAVNLVYYLWCAKIY 565

BLAST of Cucsa.161360 vs. TrEMBL
Match: A0A0A0LLZ0_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_2G374650 PE=4 SV=1)

HSP 1 Score: 1151.3 bits (2977), Expect = 0.0e+00
Identity = 578/581 (99.48%), Postives = 579/581 (99.66%), Query Frame = 1

Query: 1   MEDQLVVDENRGMEEPLLSDTDNRKGGFRTLPFIIANGALEKLASQGLSPSMILYLTKVY 60
           MEDQLVVDENRGMEEPLLSDTDNRKGGFRTLPFIIANGALEKLASQGLSPSMILYLTKVY
Sbjct: 1   MEDQLVVDENRGMEEPLLSDTDNRKGGFRTLPFIIANGALEKLASQGLSPSMILYLTKVY 60

Query: 61  GMKSAHASNVIFLWSAASNFTPIICAFLADSYFGRFPMIAAGSIFSFLGMFVLWLTAMIP 120
           GMKSAH+SNVIFLWSAASNFTPIICAFLADSYFGRFPMIAAGSIFSFLGMFVLWLTAMIP
Sbjct: 61  GMKSAHSSNVIFLWSAASNFTPIICAFLADSYFGRFPMIAAGSIFSFLGMFVLWLTAMIP 120

Query: 121 QARPFCDEISGHCDAPSTSQLLLLYSSYAIMSVGSGCLQASYLAFGADQLYRKNKSNSGI 180
           QARPFCDEISGHCDAPSTSQLLLLYSSYAIMSVGSGCLQASYLAFGADQLYRKNKSNSGI
Sbjct: 121 QARPFCDEISGHCDAPSTSQLLLLYSSYAIMSVGSGCLQASYLAFGADQLYRKNKSNSGI 180

Query: 181 LDSYFNVCYISAALGTLVGMSCIVYIQDRMGWGMGFGVPVALMFLATITFLSASPLYLKS 240
           LDSYFNVCYISAALGTLVGMSCIVYIQDRMGWGMGFGVPVALMFLATITFLSASPLYLKS
Sbjct: 181 LDSYFNVCYISAALGTLVGMSCIVYIQDRMGWGMGFGVPVALMFLATITFLSASPLYLKS 240

Query: 241 KPSKSWCAGLVQVVFAAYKKRHTQIPFVGTSEMYHHENESPCTLPSNKLRFLNKACIIRN 300
           KPSKSWCAGLVQVVFAAYKKRHTQIPFVGTSEMYHHENESPCTLPSNKLRFLNKACIIRN
Sbjct: 241 KPSKSWCAGLVQVVFAAYKKRHTQIPFVGTSEMYHHENESPCTLPSNKLRFLNKACIIRN 300

Query: 301 SKEELTLDGKASNPWSLCTVEQVENLKALIRIIPLWSTGILVSASLSQSFYVLQVASMNR 360
           SKEELTLDGKASNPWSLCTVEQVENLKALIRIIPLWSTGILVSASLSQSFYVLQVASMNR
Sbjct: 301 SKEELTLDGKASNPWSLCTVEQVENLKALIRIIPLWSTGILVSASLSQSFYVLQVASMNR 360

Query: 361 HLTSSFEVPAGSFSAIIVVSLIIWIALYDRLILPLASKCRGKPTRLCAKTRMGMGILCCC 420
           HLTSSFEVPAGSFSAIIVVSLIIWIALYDRLILPLASKCRGKPTRLCAKTRMGMGILCC 
Sbjct: 361 HLTSSFEVPAGSFSAIIVVSLIIWIALYDRLILPLASKCRGKPTRLCAKTRMGMGILCCG 420

Query: 421 LSLAVSAIVEGDRRALAIKEGFSDNPNAVVSMSAFWTLPRYILFGIAEALNAIGQIEFFY 480
           LSLAVSAIVEGDRRALAIKEGFSDNPNAVVSMSAFWTLPRYILFGIAEALNAIGQIEFFY
Sbjct: 421 LSLAVSAIVEGDRRALAIKEGFSDNPNAVVSMSAFWTLPRYILFGIAEALNAIGQIEFFY 480

Query: 481 NELPKAMSSVATSLLGLNSSIGNLAASFIMTTVDNLSKSVGVKSWVSSNINEGHSDYYYW 540
           NELPKAMSSVATSLLGLNSSIGNLAASFIMTTVDNLSKSVGVKSWVSSNINEGHSDYYYW
Sbjct: 481 NELPKAMSSVATSLLGLNSSIGNLAASFIMTTVDNLSKSVGVKSWVSSNINEGHSDYYYW 540

Query: 541 LLSGLLFANFLYYLACSKSYGPSNEESVGRANAEDYKNTVN 582
           LLSGLLFANFLYYLACSKSYGPSNEESVGRANAEDYKNT N
Sbjct: 541 LLSGLLFANFLYYLACSKSYGPSNEESVGRANAEDYKNTAN 581

BLAST of Cucsa.161360 vs. TrEMBL
Match: A0A0A0LPT0_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_2G374640 PE=4 SV=1)

HSP 1 Score: 976.9 bits (2524), Expect = 1.1e-281
Identity = 492/581 (84.68%), Postives = 525/581 (90.36%), Query Frame = 1

Query: 1   MEDQLVVDENRGMEEPLLSDTDNRKGGFRTLPFIIANGALEKLASQGLSPSMILYLTKVY 60
           MED LVV ENRGMEEPLLS TD+ KGG RTLPFIIA+GALEKLAS GLSPSMILYLT+VY
Sbjct: 1   MEDPLVVGENRGMEEPLLSGTDDCKGGIRTLPFIIASGALEKLASMGLSPSMILYLTEVY 60

Query: 61  GMKSAHASNVIFLWSAASNFTPIICAFLADSYFGRFPMIAAGSIFSFLGMFVLWLTAMIP 120
           GMKS  AS+VIFLW+AA+ FTPIICAFLADSYFGRF MI AGSIFS LGMFVLWLTAMIP
Sbjct: 61  GMKSVQASSVIFLWTAATYFTPIICAFLADSYFGRFMMIVAGSIFSCLGMFVLWLTAMIP 120

Query: 121 QARPFCDEISGHCDAPSTSQLLLLYSSYAIMSVGSGCLQASYLAFGADQLYRKNKSNSGI 180
           QARPFCDEISGHC+APST QLL LYSSYAIMS+GSGCLQ+S  AFGADQLY++NKSNSGI
Sbjct: 121 QARPFCDEISGHCNAPSTPQLLFLYSSYAIMSIGSGCLQSSNFAFGADQLYKENKSNSGI 180

Query: 181 LDSYFNVCYISAALGTLVGMSCIVYIQDRMGWGMGFGVPVALMFLATITFLSASPLYLKS 240
           LD YFN+CYIS ++GTLVGMSCIVYIQDRMGW MGFGVP+ALM LAT+TFLSAS LYLKS
Sbjct: 181 LDIYFNLCYISVSVGTLVGMSCIVYIQDRMGWEMGFGVPIALMLLATVTFLSASSLYLKS 240

Query: 241 KPSKSWCAGLVQVVFAAYKKRHTQIPFVGTSEMYHHENESPCTLPSNKLRFLNKACIIRN 300
            PSKSWCAGLV VVFAAYKKR  QIPFVGTSEMYHHEN S CTLPS+KLRFLNKACII+N
Sbjct: 241 VPSKSWCAGLVHVVFAAYKKRRMQIPFVGTSEMYHHENGSSCTLPSDKLRFLNKACIIKN 300

Query: 301 SKEELTLDGKASNPWSLCTVEQVENLKALIRIIPLWSTGILVSASLSQSFYVLQVASMNR 360
            +EEL  DGKASNPWSLCTVEQVENLKALIRIIPLWSTGILVSAS+SQS YVLQVASM+R
Sbjct: 301 YEEELISDGKASNPWSLCTVEQVENLKALIRIIPLWSTGILVSASMSQSVYVLQVASMDR 360

Query: 361 HLTSSFEVPAGSFSAIIVVSLIIWIALYDRLILPLASKCRGKPTRLCAKTRMGMGILCCC 420
           HLTSSFEVPAGSF A++VV +IIWI LYDRLILPLAS+ RGKPTRL  KTRMGM I  C 
Sbjct: 361 HLTSSFEVPAGSFIAMLVVFVIIWIILYDRLILPLASRYRGKPTRLSGKTRMGMAIPFCI 420

Query: 421 LSLAVSAIVEGDRRALAIKEGFSDNPNAVVSMSAFWTLPRYILFGIAEALNAIGQIEFFY 480
           LSLAVSAIVEG RRALAIKEGFSD+PNAVVSMSAFWTLPRYILFGIAEA  AIGQ+EFFY
Sbjct: 421 LSLAVSAIVEGYRRALAIKEGFSDDPNAVVSMSAFWTLPRYILFGIAEAFYAIGQLEFFY 480

Query: 481 NELPKAMSSVATSLLGLNSSIGNLAASFIMTTVDNLSKSVGVKSWVSSNINEGHSDYYYW 540
           NELPKAMSSVATSLLGL+S  GNLAASFIMTTVDN SK++GVKSWVSSNINEGH+DYYYW
Sbjct: 481 NELPKAMSSVATSLLGLSSCAGNLAASFIMTTVDNFSKTIGVKSWVSSNINEGHNDYYYW 540

Query: 541 LLSGLLFANFLYYLACSKSYGPSNEESVGRANAEDYKNTVN 582
           LL GLL ANF YYLAC+ SYGPS EES   +NAEDY NTVN
Sbjct: 541 LLFGLLVANFFYYLACNNSYGPSKEESEDISNAEDYNNTVN 581

BLAST of Cucsa.161360 vs. TrEMBL
Match: A0A061GK08_THECC (Major facilitator superfamily protein OS=Theobroma cacao GN=TCM_037174 PE=4 SV=1)

HSP 1 Score: 639.4 bits (1648), Expect = 4.2e-180
Identity = 315/557 (56.55%), Postives = 412/557 (73.97%), Query Frame = 1

Query: 8   DENRGMEEPLLSDTDNRKGGFRTLPFIIANGALEKLASQGLSPSMILYLTKVYGMKSAHA 67
           DE + + EPLL      KGGFRTLPFI+AN A E++A+ GLSP+MILYLT+ YGM++A A
Sbjct: 7   DEKKTIREPLLITPSLSKGGFRTLPFILANEAFERVATLGLSPNMILYLTREYGMETARA 66

Query: 68  SNVIFLWSAASNFTPIICAFLADSYFGRFPMIAAGSIFSFLGMFVLWLTAMIPQARPFCD 127
           + VIF+W+AA+NFTPI+ AF+ADSY G++ MI  GSI SFLGM +LWLTAM PQARP+CD
Sbjct: 67  AKVIFIWTAANNFTPILGAFVADSYVGKYRMIGLGSILSFLGMVLLWLTAMFPQARPYCD 126

Query: 128 EISGHCDAPSTSQLLLLYSSYAIMSVGSGCLQASYLAFGADQLYRK-NKSNSGILDSYFN 187
           + S  C++P+  QLL LY S  +MS+G+G +++S L FGADQL +  N  N+  L S+F+
Sbjct: 127 QFSSICESPTAPQLLFLYFSLGLMSIGTGGIRSSSLVFGADQLNKGINPENAETLQSFFS 186

Query: 188 VCYISAALGTLVGMSCIVYIQDRMGWGMGFGVPVALMFLATITFLSASPLYLKSKPSKSW 247
             Y S +   L+ ++ IVYIQD +GW MGFGVPV LMF++ ++F  AS LY+K K   S 
Sbjct: 187 WYYASVSFSALIAVTGIVYIQDNLGWKMGFGVPVMLMFISALSFYLASSLYIKLKARTSL 246

Query: 248 CAGLVQVVFAAYKKRHTQIPFVGTSEMYHHENESPCTLPSNKLRFLNKACIIRNSKEELT 307
             G  QV+ A+++ RH  +P   T E+Y     S   +PS KLRFLNKAC+I+N +++LT
Sbjct: 247 FTGFAQVLVASFRNRHINLPSQATDEVYCLRKGSRLYMPSEKLRFLNKACVIKNPQQDLT 306

Query: 308 LDGKASNPWSLCTVEQVENLKALIRIIPLWSTGILVSASLSQ-SFYVLQVASMNRHLTSS 367
            DG ASNPWSLCTV+QVE LKALIR+IPL+STGI++S +L+Q SF V+Q  +M+RH+T  
Sbjct: 307 SDGTASNPWSLCTVDQVEELKALIRVIPLFSTGIMISVALTQSSFTVIQAGTMDRHITPK 366

Query: 368 FEVPAGSFSAIIVVSLIIWIALYDRLILPLASKCRGKPTRLCAKTRMGMGILCCCLSLAV 427
           FE+PAGSFS  +++SLI WIA YDR+ LPLASK +GKP RL  K R G+GILC C S+  
Sbjct: 367 FEIPAGSFSMFLMISLIAWIAFYDRIALPLASKMKGKPVRLGLKQRTGIGILCSCASMVA 426

Query: 428 SAIVEGDRRALAIKEGFSDNPNAVVSMSAFWTLPRYILFGIAEALNAIGQIEFFYNELPK 487
            AIVE  RR +AI+EG SD P A+V MSA W LP  +L G+AEA   +GQIEFFY ELPK
Sbjct: 427 LAIVEYIRREIAIQEGLSDEPQAMVHMSALWLLPYNVLSGLAEAFAGVGQIEFFYAELPK 486

Query: 488 AMSSVATSLLGLNSSIGNLAASFIMTTVDNLSKSVGVKSWVSSNINEGHSDYYYWLLSGL 547
            MSS+ ++L GL + + +L ASFI + VD+++K  G +SWVSSN+N+GH DYYYWLL GL
Sbjct: 487 TMSSIGSNLFGLGAFVASLVASFITSAVDDVTKR-GGESWVSSNVNKGHYDYYYWLLFGL 546

Query: 548 LFANFLYYLACSKSYGP 563
              NF+Y+LACSK+YGP
Sbjct: 547 SILNFMYFLACSKAYGP 562

BLAST of Cucsa.161360 vs. TrEMBL
Match: A0A0B0NCZ4_GOSAR (Uncharacterized protein OS=Gossypium arboreum GN=F383_17450 PE=4 SV=1)

HSP 1 Score: 629.8 bits (1623), Expect = 3.3e-177
Identity = 309/576 (53.65%), Postives = 423/576 (73.44%), Query Frame = 1

Query: 8   DENRGMEEPLLSDTDNRKGGFRTLPFIIANGALEKLASQGLSPSMILYLTKVYGMKSAHA 67
           DEN  + EP LS + + KGGFRTLPFI+A  A E++A+ GLSP+MI+YL + +GM++A A
Sbjct: 6   DENNKIREPFLSSSPS-KGGFRTLPFILATEAFERVATLGLSPNMIVYLRREFGMETAKA 65

Query: 68  SNVIFLWSAASNFTPIICAFLADSYFGRFPMIAAGSIFSFLGMFVLWLTAMIPQARPFCD 127
           +NVIF WSAA+NFTPII AF+ADSY G++ MI  GS+  FLGM +LW TA+IPQARP+CD
Sbjct: 66  ANVIFFWSAATNFTPIIGAFIADSYMGKYRMIGFGSVLCFLGMILLWSTAIIPQARPYCD 125

Query: 128 EISGHCDAPSTSQLLLLYSSYAIMSVGSGCLQASYLAFGADQL-YRKNKSNSGILDSYFN 187
           + +  C+AP+T QLLLLY S  ++S+G+G +++S +AFGADQL  R N  N+  L S+F 
Sbjct: 126 QFNTTCEAPTTPQLLLLYCSLGLISIGAGGIRSSCMAFGADQLDKRNNPENTKTLQSFFG 185

Query: 188 VCYISAALGTLVGMSCIVYIQDRMGWGMGFGVPVALMFLATITFLSASPLYLKSKPSKSW 247
             Y +     L+ ++ IVY+QD +GWGMGFG+PV LMF + I+F  AS  Y+K K   S 
Sbjct: 186 WYYATVTFSALIAVTLIVYVQDNLGWGMGFGIPVMLMFGSAISFFLASSFYIKLKAKTSL 245

Query: 248 CAGLVQVVFAAYKKRHTQIPFVGTSEMYHHENESPCTLPSNKLRFLNKACIIRNSKEELT 307
             GL QV+ A+++ RH ++P   T+E+YH    S   +PS KLRFLNKAC+I++ +E+LT
Sbjct: 246 LTGLAQVLVASFRNRHIELPSHATNEVYHIRKGSMLQVPSEKLRFLNKACVIKDPQEDLT 305

Query: 308 LDGKASNPWSLCTVEQVENLKALIRIIPLWSTGILVSASLSQ-SFYVLQVASMNRHLTSS 367
            +G ASNPW+LCTV+QVE+LKALIR++PL S GI++S ++ Q SF V+Q  +M+RH+T +
Sbjct: 306 SNGDASNPWNLCTVDQVEDLKALIRVMPLCSAGIMMSVNVYQGSFMVIQAGTMDRHVTPN 365

Query: 368 FEVPAGSFSAIIVVSLIIWIALYDRLILPLASKCRGKPTRLCAKTRMGMGILCCCLSLAV 427
           FE+PAGSFS  +++S+++WI  YDR+ LPLASK +GK  RL  K RMG+G+LC C S+  
Sbjct: 366 FEIPAGSFSMFMMISIVVWIPFYDRIALPLASKIKGKSVRLGLKQRMGIGLLCSCASMVA 425

Query: 428 SAIVEGDRRALAIKEGFSDNPNAVVSMSAFWTLPRYILFGIAEALNAIGQIEFFYNELPK 487
           SAIVE  RR +AI+EG SD P AVV MSA W LP YIL G+AEA N IGQIEF Y+ELPK
Sbjct: 426 SAIVEYTRRKIAIEEGLSDEPPAVVHMSALWILPFYILGGLAEAFNGIGQIEFCYSELPK 485

Query: 488 AMSSVATSLLGLNSSIGNLAASFIMTTVDNLSKSVGVKSWVSSNINEGHSDYYYWLLSGL 547
            MS++A ++ G  + I NL ASFI + V+N++K  G +SW+SSN+N+GH DYYY LL+GL
Sbjct: 486 TMSTIAANIYGFGTFISNLVASFITSMVNNVTKR-GGESWISSNMNKGHYDYYYCLLAGL 545

Query: 548 LFANFLYYLACSKSYGP---SNEESVGRANAEDYKN 579
              NF+Y+L+C K+YGP    N+    ++   DY++
Sbjct: 546 SMLNFIYFLSCCKAYGPCHGDNKNQAEKSKKGDYES 579

BLAST of Cucsa.161360 vs. TrEMBL
Match: A0A0B2PRK2_GLYSO (Putative peptide transporter OS=Glycine soja GN=glysoja_017811 PE=4 SV=1)

HSP 1 Score: 629.4 bits (1622), Expect = 4.3e-177
Identity = 303/553 (54.79%), Postives = 426/553 (77.03%), Query Frame = 1

Query: 13  MEEPLLS-DTDNRKGGFRTLPFIIANGALEKLASQGLSPSMILYLTKVYGMKSAHASNVI 72
           +EEPLLS    N KGGFRTLPFIIAN A E+LAS GL P+MILYLT+ YGM++A A+N +
Sbjct: 5   IEEPLLSKQNSNAKGGFRTLPFIIANEAFERLASLGLMPNMILYLTREYGMQTAGATNFL 64

Query: 73  FLWSAASNFTPIICAFLADSYFGRFPMIAAGSIFSFLGMFVLWLTAMIPQARPFCDEISG 132
            LWSAASNFTP + A L+DSY GR+ MIA GSI S LGM +LWLT +IP ++P C++ + 
Sbjct: 65  LLWSAASNFTPFVGAVLSDSYVGRYSMIAFGSIASLLGMVLLWLTTLIPLSKPLCNQFTN 124

Query: 133 HCD-APSTSQLLLLYSSYAIMSVGSGCLQASYLAFGADQLYRKNKSNSGILDSYFNVCYI 192
            C+ +P+T  LL+L+SS+A+MS+G+G +++S LAFG DQL +++K N+GI +SYF+  Y 
Sbjct: 125 SCNNSPTTIHLLILHSSFALMSIGAGGIRSSSLAFGVDQLSKRDK-NAGIKESYFSWYYA 184

Query: 193 SAALGTLVGMSCIVYIQDRMGWGMGFGVPVALMFLATITFLSASPLYLKSKPSKSWCAGL 252
             A+ +L+G++ +VYIQD MGW +GFG+PV LMF+AT++F  ASP Y+  +  ++  +GL
Sbjct: 185 IVAMSSLIGLTVVVYIQDNMGWAVGFGIPVILMFVATVSFFLASPFYVMVEVKRNMLSGL 244

Query: 253 VQVVFAAYKKRHTQIPFVGTSEMYHHENESPCTLPSNKLRFLNKACIIRNSKEELTLDGK 312
            QV+ A+YK R  Q+P    + +YH E +S   +P+ KLRFLNKAC+IRNS ++LT +G+
Sbjct: 245 AQVLVASYKNRLLQLPQETENGIYHLEKDSDLLMPTEKLRFLNKACLIRNSLQDLTPEGR 304

Query: 313 ASNPWSLCTVEQVENLKALIRIIPLWSTGILVSASLSQ-SFYVLQVASMNRHLTSSFEVP 372
           A NPW+LCTV+QVE LKALI+I+P+WSTGI++  ++SQ S  VL+ +SM+RH+TS+FE+P
Sbjct: 305 ALNPWNLCTVDQVEELKALIKIVPIWSTGIMMGVNISQGSLLVLEASSMDRHITSNFEIP 364

Query: 373 AGSFSAIIVVSLIIWIALYDRLILPLASKCRGKPTRLCAKTRMGMGILCCCLSLAVSAIV 432
           +GSF   ++VSL++W+ +YDR+++P+ASK +G P  + AK +MG+G+L  C+++A  A+V
Sbjct: 365 SGSFVTFMIVSLVLWVIIYDRILVPVASKIKGSPACIGAKQKMGIGLLTGCIAIASLAVV 424

Query: 433 EGDRRALAIKEGFSDNPNAVVSMSAFWTLPRYILFGIAEALNAIGQIEFFYNELPKAMSS 492
           E  RR +AI++G+ D P AVV+MSA W LPR IL G+AEAL  +GQ EFF  ELP++MSS
Sbjct: 425 EDIRRKIAIEKGYEDQPQAVVNMSALWLLPRQILNGLAEALGVVGQNEFFLTELPQSMSS 484

Query: 493 VATSLLGLNSSIGNLAASFIMTTVDNLSKSVGVKSWVSSNINEGHSDYYYWLLSGLLFAN 552
           +A++L GL SS+ NL ASFI++ VDN++   G +SW+SSNIN+GH DYYY L+  L F N
Sbjct: 485 LASTLNGLGSSVANLVASFILSVVDNVTGGGGHESWLSSNINKGHYDYYYTLICALCFVN 544

Query: 553 FLYYLACSKSYGP 563
           F+Y+L CSKSYGP
Sbjct: 545 FVYFLYCSKSYGP 556

BLAST of Cucsa.161360 vs. TAIR10
Match: AT1G52190.1 (AT1G52190.1 Major facilitator superfamily protein)

HSP 1 Score: 570.1 bits (1468), Expect = 1.6e-162
Identity = 287/547 (52.47%), Postives = 388/547 (70.93%), Query Frame = 1

Query: 25  KGGFRTLPFIIANGALEKLASQGLSPSMILYLTKVYGMKSAHASNVIFLWSAASNFTPII 84
           KGG  T+PFIIAN A EK+AS GL P+MI+YL + Y    A  +NV+F+WSAASNFTP++
Sbjct: 22  KGGIITMPFIIANEAFEKVASYGLLPNMIMYLIRDYRFGVAKGTNVLFMWSAASNFTPLL 81

Query: 85  CAFLADSYFGRFPMIAAGSIFSFLGMFVLWLTAMIPQARPF-CDEISG--HCDAPSTSQL 144
            AFL+DSY GRF  I+  S+ SFLGM +LWLTAM+PQ +P  CD  +   HC + + SQL
Sbjct: 82  GAFLSDSYLGRFLTISIASLSSFLGMVLLWLTAMLPQVKPSPCDPTAAGSHCGSSTASQL 141

Query: 145 LLLYSSYAIMSVGSGCLQASYLAFGADQLYRK-NKSNSGILDSYFNVCYISAALGTLVGM 204
            LLYS++A++S+GSG ++   LAFGADQL  K N  N  +L+S+F   Y S+A+  L+  
Sbjct: 142 ALLYSAFALISIGSGGIRPCSLAFGADQLDNKENPKNERVLESFFGWYYASSAVAVLIAF 201

Query: 205 SCIVYIQDRMGWGMGFGVPVALMFLATITFLSASPLYLKSKPSKSWCAGLVQVVFAAYKK 264
           + IVYIQ+ +GW +GFGVP  LM +A + F+ ASPLY+    +KS   GL Q + AAYKK
Sbjct: 202 TGIVYIQEHLGWKIGFGVPAVLMLIAALLFILASPLYVTRGVTKSLFTGLAQAIVAAYKK 261

Query: 265 RHTQIPFVGTS-EMYHHENESPCTLPSNKLRFLNKACIIRNSKEELTLDGKASNPWSLCT 324
           R   +P    S + Y+H  +S    PS KLRFLNKAC+I N +EE+  DG A NPW LCT
Sbjct: 262 RKLSLPDHHDSFDCYYHMKDSEIKAPSQKLRFLNKACLISNREEEIGSDGFALNPWRLCT 321

Query: 325 VEQVENLKALIRIIPLWSTGILVSASLSQS-FYVLQVASMNRHLT---SSFEVPAGSFSA 384
            ++VE LKALI++IP+WSTGI++S + SQS F +LQ  SM+R L+   SSF+VPAGSF  
Sbjct: 322 TDKVEELKALIKVIPIWSTGIMMSINTSQSSFQLLQATSMDRRLSRHGSSFQVPAGSFGM 381

Query: 385 IIVVSLIIWIALYDRLILPLASKCRGKPTRLCAKTRMGMGILCCCLSLAVSAIVEGDRRA 444
             +++L +W+ LYDR ++PLASK RG+P RL  K RMG+G+    L++A+SA+VE  RR 
Sbjct: 382 FTIIALALWVILYDRAVIPLASKIRGRPFRLSVKLRMGLGLFMSFLAMAISAMVESFRRK 441

Query: 445 LAIKEGFSDNPNAVVSMSAFWTLPRYILFGIAEALNAIGQIEFFYNELPKAMSSVATSLL 504
            AI +G+++N NAVV +SA W +P+Y+L G+AEAL AIGQ EFFY E PK+MSS+A SL 
Sbjct: 442 KAISQGYANNSNAVVDISAMWLVPQYVLHGLAEALTAIGQTEFFYTEFPKSMSSIAASLF 501

Query: 505 GLNSSIGNLAASFIMTTVDNLSKSVGVKSWVSSNINEGHSDYYYWLLSGLLFANFLYYLA 563
           GL  ++ +L AS ++  V+ L+   G +SWVS NIN+GH +YYYW+L+ + F N +YY+ 
Sbjct: 502 GLGMAVASLLASVVLNAVNELTSRNGKESWVSDNINKGHYNYYYWVLAIMSFINVIYYVI 561

BLAST of Cucsa.161360 vs. TAIR10
Match: AT3G16180.1 (AT3G16180.1 Major facilitator superfamily protein)

HSP 1 Score: 541.6 bits (1394), Expect = 6.0e-154
Identity = 281/549 (51.18%), Postives = 377/549 (68.67%), Query Frame = 1

Query: 25  KGGFRTLPFIIANGALEKLASQGLSPSMILYLTKVYGMKSAHASNVIFLWSAASNFTPII 84
           KGG  T+PFIIAN   EK+AS GL  +MILYL   Y +       V+F+W AA+NF P++
Sbjct: 24  KGGLLTMPFIIANEGFEKVASYGLLQNMILYLMSDYRLGLVKGQTVLFMWVAATNFMPLV 83

Query: 85  CAFLADSYFGRFPMIAAGSIFSFLGMFVLWLTAMIPQARPF-CDEISG-HCDAPSTSQLL 144
            AFL+DSY GRF  I   S+ S LGM VLWLTAM+PQ +P  C   +G +C + ++SQL 
Sbjct: 84  GAFLSDSYLGRFLTIVIASLSSLLGMVVLWLTAMLPQVKPSPCVATAGTNCSSATSSQLA 143

Query: 145 LLYSSYAIMSVGSGCLQASYLAFGADQLYRK-NKSNSGILDSYFNVCYISAALGTLVGMS 204
           LLY+++A++S+GSG ++   LAFGADQL  K N  N  +L+S+F   Y S+++  L+  +
Sbjct: 144 LLYTAFALISIGSGGIRPCSLAFGADQLDNKENPKNERVLESFFGWYYASSSVAVLIAFT 203

Query: 205 CIVYIQDRMGWGMGFGVPVALMFLATITFLSASPLYLKSKPSKSWCAGLVQVVFAAYKKR 264
            IVYIQD +GW +GFG+P  LM LA   F+ ASPLY+K   SKS   GL QVV AAY KR
Sbjct: 204 VIVYIQDHLGWKIGFGIPAILMLLAGFLFVFASPLYVKRDVSKSLFTGLAQVVAAAYVKR 263

Query: 265 HTQIPFVGTS-EMYHHENESPCTLPSNKLRFLNKACIIRNSKEELTLDGKASNPWSLCTV 324
           +  +P    S + Y+   +S    PS+KLRFLNKAC I N  E+L  DG A N W LCT 
Sbjct: 264 NLTLPDHHDSRDCYYRLKDSELKAPSDKLRFLNKACAISNRDEDLGSDGLALNQWRLCTT 323

Query: 325 EQVENLKALIRIIPLWSTGILVSASLSQ-SFYVLQVASMNRHLT--SSFEVPAGSFSAII 384
           +QVE LKAL+++IP+WSTGI++S ++SQ SF +LQ  SM+R L+  S+F++PAGSF    
Sbjct: 324 DQVEKLKALVKVIPVWSTGIMMSINVSQNSFQLLQAKSMDRRLSSNSTFQIPAGSFGMFT 383

Query: 385 VVSLIIWIALYDRLILPLASKCRGKPTRLCAKTRMGMGILCCCLSLAVSAIVEGDRRALA 444
           +++LI W+ LYDR ILPLASK RG+P R+  K RMG+G+    L++AVSA VE  RR  A
Sbjct: 384 IIALISWVVLYDRAILPLASKIRGRPVRVNVKIRMGLGLFISFLAMAVSATVEHYRRKTA 443

Query: 445 IKEGFSDNPNAVVSMSAFWTLPRYILFGIAEALNAIGQIEFFYNELPKAMSSVATSLLGL 504
           I +G +++ N+ VS+SA W +P+Y+L G+AEAL  IGQ EFFY E PK+MSS+A SL GL
Sbjct: 444 ISQGLANDANSTVSISAMWLVPQYVLHGLAEALTGIGQTEFFYTEFPKSMSSIAASLFGL 503

Query: 505 NSSIGNLAASFIMTTVDNLSKSVGVKSWVSSNINEGHSDYYYWLLSGLLFANFLYYLACS 564
             ++ N+ AS I+  V N SK  G  SW+  NIN+GH DYYYW+L+ L F N +YY+ CS
Sbjct: 504 GMAVANILASVILNAVKNSSKQ-GNVSWIEDNINKGHYDYYYWVLAILSFVNVIYYVVCS 563

Query: 565 KSYGPSNEE 567
            SYGP+ ++
Sbjct: 564 WSYGPTVDQ 571

BLAST of Cucsa.161360 vs. TAIR10
Match: AT1G69870.1 (AT1G69870.1 nitrate transporter 1.7)

HSP 1 Score: 354.0 bits (907), Expect = 1.8e-97
Identity = 192/550 (34.91%), Postives = 317/550 (57.64%), Query Frame = 1

Query: 22  DNRKGGFRTLPFIIANGALEKLASQGLSPSMILYLTKVYGMKSAHASNVIFLWSAASNFT 81
           + + GG+R + FI+ N  LE+L S GL  + ++YLTKV+ ++   A+NVI +WS  +N T
Sbjct: 49  EKKPGGWRAVSFILGNETLERLGSIGLLANFMVYLTKVFHLEQVDAANVINIWSGFTNLT 108

Query: 82  PIICAFLADSYFGRFPMIAAGSIFSFLGMFVLWLTAMIPQARPFC----DEISGHCDAPS 141
           P++ A+++D+Y GRF  IA  S  + LG+  + LTA  PQ  P      D +S  C  P+
Sbjct: 109 PLVGAYISDTYVGRFKTIAFASFATLLGLITITLTASFPQLHPASCNSQDPLS--CGGPN 168

Query: 142 TSQLLLLYSSYAIMSVGSGCLQASYLAFGADQLYRKNKSNSGILDSYFNVCYISAALGTL 201
             Q+ +L      +SVGSG ++   + FG DQ  ++ +     + S+FN  Y++  +  +
Sbjct: 169 KLQIGVLLLGLCFLSVGSGGIRPCSIPFGVDQFDQRTEEGVKGVASFFNWYYMTFTVVLI 228

Query: 202 VGMSCIVYIQDRMGWGMGFGVPVALMFLATITFLSASPLYLKSKPSKSWCAGLVQVVFAA 261
           +  + +VYIQD++ W +GF +P  LM LA + F +    Y+  KP  S  +G+ QV+ AA
Sbjct: 229 ITQTVVVYIQDQVSWIIGFSIPTGLMALAVVMFFAGMKRYVYVKPEGSIFSGIAQVIVAA 288

Query: 262 YKKRHTQIPFVGTSEMYHHENESPCTL-----PSNKLRFLNKACIIRNSKEELTLDGKAS 321
            KKR  ++P      + +++     ++      SN+ R L+KA ++     +LT +G  +
Sbjct: 289 RKKRKLKLPAEDDGTVTYYDPAIKSSVLSKLHRSNQFRCLDKAAVVIEG--DLTPEGPPA 348

Query: 322 NPWSLCTVEQVENLKALIRIIPLWSTGILVSASLSQ--SFYVLQVASMNRHLTSSFEVPA 381
           + W LC+V++VE +K LIRI+P+WS GI+  A+++   +F V Q   M+R+L   FE+PA
Sbjct: 349 DKWRLCSVQEVEEVKCLIRIVPIWSAGIISLAAMTTQGTFTVSQALKMDRNLGPKFEIPA 408

Query: 382 GSFSAIIVVSLIIWIALYDRLILPLASKCRGKPTRLCAKTRMGMGILCCCLSLAVSAIVE 441
           GS S I ++++ I++  YDR+ +P   +  G  + +    R+G GI+    S+ V+ IVE
Sbjct: 409 GSLSVISLLTIGIFLPFYDRVFVPFMRRITGHKSGITLLQRIGTGIVFAIFSMIVAGIVE 468

Query: 442 GDRRALAIKEGFSDNPNAVVSMSAFWTLPRYILFGIAEALNAIGQIEFFYNELPKAMSSV 501
             RR  +I  G   +P  +  MS FW  P+ IL G+ EA N IGQIEFF ++ P+ M S+
Sbjct: 469 RMRRIRSINAG---DPTGMTPMSVFWLSPQLILMGLCEAFNIIGQIEFFNSQFPEHMRSI 528

Query: 502 ATSLLGLNSSIGNLAASFIMTTVDNLSKSVGVKSWVSSNINEGHSDYYYWLLSGLLFANF 561
           A SL  L+ +  +  +SF++T V   S       W++ N+N G  DY+Y+L++ L   N 
Sbjct: 529 ANSLFSLSFAGSSYLSSFLVTVVHKFSGGHDRPDWLNKNLNAGKLDYFYYLIAVLGVVNL 588

BLAST of Cucsa.161360 vs. TAIR10
Match: AT3G47960.1 (AT3G47960.1 Major facilitator superfamily protein)

HSP 1 Score: 354.0 bits (907), Expect = 1.8e-97
Identity = 194/553 (35.08%), Postives = 315/553 (56.96%), Query Frame = 1

Query: 27  GFRTLPFIIANGALEKLASQGLSPSMILYLTKVYGMKSAHASNVIFLWSAASNFTPIICA 86
           G++ +PFII N   EKL   G   ++++YLT V+ +KS  A+ +I  +S   NF   I A
Sbjct: 64  GWKVMPFIIGNETFEKLGIIGTLSNLLVYLTSVFNLKSYTAATIINAFSGTINFGTFIAA 123

Query: 87  FLADSYFGRFPMIAAGSIFSFLGMFVLWLTAMIPQARPFCDEISGHCDAPSTSQLLLLYS 146
           FL D+YFGR+  ++   I  FLG FV+ LTA IP   P        C+ PS  Q+L L  
Sbjct: 124 FLCDTYFGRYKTLSVAVIACFLGSFVILLTAAIPSLHPVACGNKISCEGPSVGQILFLLM 183

Query: 147 SYAIMSVGSGCLQASYLAFGADQLYRKNKSNSGILDSYFNVCYISAALGTLVGMSCIVYI 206
               + VG+G ++   LAFGADQ   K++S    ++S+FN  + +     ++ ++ +VYI
Sbjct: 184 GLGFLVVGAGGIRPCNLAFGADQFNPKSESGKKGINSFFNWYFFTFTFAQIISLTAVVYI 243

Query: 207 QDRMGWGMGFGVPVALMFLATITFLSASPLYLKSKPSKSWCAGLVQVVFAAYKKRHTQIP 266
           Q  + W +G  +PVALMFLA + F +   LY+K K S S  AG+ +V+ AA KKR  +  
Sbjct: 244 QSNVSWTIGLIIPVALMFLACVIFFAGDRLYVKVKASGSPLAGIARVIAAAIKKRGLKPV 303

Query: 267 FVGTSEMYHH--ENESPCTLP-SNKLRFLNKACIIRNSKEELTLDGKASNPWSLCTVEQV 326
                 +Y+H   N +  TL  +++ RFL+KA I+   +E+L  DG AS+PW LCT++QV
Sbjct: 304 KQPWVNLYNHIPSNYANTTLKYTDQFRFLDKAAIM-TPEEKLNSDGTASDPWKLCTLQQV 363

Query: 327 ENLKALIRIIPLW--STGILVSASLSQSFYVLQVASMNRHLTS-SFEVPAGSFSAIIVVS 386
           E +K ++R+IP+W  ST   ++ ++  ++ V Q    +R L S  F +PA ++   ++  
Sbjct: 364 EEVKCIVRVIPIWFASTIYYLAITIQMTYPVFQALQSDRRLGSGGFRIPAATYVVFLMTG 423

Query: 387 LIIWIALYDRLILPLASKCRGKPTRLCAKTRMGMGILCCCLSLAVSAIVEGDRRALAIKE 446
           + ++I  YDR+++P   +  G  T +    R+G G     +SL VS  +E  RR  A+ +
Sbjct: 424 MTVFIIFYDRVLVPSLRRVTGLETGISLLQRIGAGFTFAIMSLLVSGFIEERRRNFALTK 483

Query: 447 ---GFSDNPNAVVSMSAFWTLPRYILFGIAEALNAIGQIEFFYNELPKAMSSVATSLLGL 506
              G +     + SMSA W +P+  L GIAEA  AIGQ+EF+Y + P+ M S A S+  +
Sbjct: 484 PTLGMAPRTGEISSMSALWLIPQLTLAGIAEAFAAIGQMEFYYKQFPENMKSFAGSIFYV 543

Query: 507 NSSIGNLAASFIMTTVDNLSKSVGVKSWVSSNINEGHSDYYYWLLSGLLFANFLYYLACS 566
            + + +  ASF+++TV   +      +W++ ++N+   DY+Y++L+GL+  N  Y+L  +
Sbjct: 544 GAGVSSYLASFLISTVHRTTAHSPSGNWLAEDLNKAKLDYFYFMLTGLMVVNMAYFLLMA 603

Query: 567 K--SYGPSNEESV 569
           +   Y   N+E +
Sbjct: 604 RWYRYKGGNDEDI 615

BLAST of Cucsa.161360 vs. TAIR10
Match: AT1G68570.1 (AT1G68570.1 Major facilitator superfamily protein)

HSP 1 Score: 353.2 bits (905), Expect = 3.0e-97
Identity = 201/556 (36.15%), Postives = 322/556 (57.91%), Query Frame = 1

Query: 14  EEPLLSDTDNR-KGGFRTLPFIIANGALEKLASQGLSPSMILYLTKVYGMKSAHASNVIF 73
           EE  L    NR KGG  T+PFI AN   EKLA  G   +MI YLT    +    A+N + 
Sbjct: 12  EEKQLHGRPNRPKGGLITMPFIFANEICEKLAVVGFHANMISYLTTQLHLPLTKAANTLT 71

Query: 74  LWSAASNFTPIICAFLADSYFGRFPMIAAGSIFSFLGMFVLWLTAMIPQARPFCDEISGH 133
            ++  S+ TP++ AF+ADS+ GRF  I   SI   +GM +L ++A+IP  RP   +    
Sbjct: 72  NFAGTSSLTPLLGAFIADSFAGRFWTITFASIIYQIGMTLLTISAIIPTLRPPPCKGEEV 131

Query: 134 CDAPSTSQLLLLYSSYAIMSVGSGCLQASYLAFGADQLYRKNKSNSGILDSYFNVCYISA 193
           C    T+QL +LY +  + ++GSG ++   +AFGADQ    + + +    +YFN  Y   
Sbjct: 132 CVVADTAQLSILYVALLLGALGSGGIRPCVVAFGADQFDESDPNQTTKTWNYFNWYYFCM 191

Query: 194 ALGTLVGMSCIVYIQDRMGWGMGFGVPVALMFLATITFLSASPLYLKSKPSKSWCAGLVQ 253
               L+ ++ +V+IQD +GWG+G G+P   MFL+ I F+    LY    P+ S    L+Q
Sbjct: 192 GAAVLLAVTVLVWIQDNVGWGLGLGIPTVAMFLSVIAFVGGFQLYRHLVPAGSPFTRLIQ 251

Query: 254 VVFAAYKKRHTQIPFVGTSEMYHHENESPCTL-----PSNKLRFLNKACIIRNSKEELTL 313
           V  AA++KR  ++    +   ++ E ++P +L      +  + FL+KA I+  ++E+   
Sbjct: 252 VGVAAFRKRKLRMVSDPSLLYFNDEIDAPISLGGKLTHTKHMSFLDKAAIV--TEEDNLK 311

Query: 314 DGKASNPWSLCTVEQVENLKALIRIIPLWSTGILVSASLSQ--SFYVLQVASMNRHLTSS 373
            G+  N W L TV +VE LK++IR+ P+ ++GIL+  + +Q  +F + Q  +MNRHLT+S
Sbjct: 312 PGQIPNHWRLSTVHRVEELKSVIRMGPIGASGILLITAYAQQGTFSLQQAKTMNRHLTNS 371

Query: 374 FEVPAGSFSAIIVVSLIIWIALYDRLILPLASKCRGKPTRLCAKTRMGMGILCCCLSLAV 433
           F++PAGS S    V+++  I  YDR+ + +A K  G    +    RMG+G +   ++  V
Sbjct: 372 FQIPAGSMSVFTTVAMLTTIIFYDRVFVKVARKFTGLERGITFLHRMGIGFVISIIATLV 431

Query: 434 SAIVEGDRRALAIKEGFSDNPNAVVSMSAFWTLPRYILFGIAEALNAIGQIEFFYNELPK 493
           +  VE  R+++AI+ G  D P+ +V +S  W +P+Y L G+AEA  +IG +EFFY++ P+
Sbjct: 432 AGFVEVKRKSVAIEHGLLDKPHTIVPISFLWLIPQYGLHGVAEAFMSIGHLEFFYDQAPE 491

Query: 494 AMSSVATSLLGLNSSIGNLAASFIMTTVDNLSKSVGVKSWV-SSNINEGHSDYYYWLLSG 553
           +M S AT+L  +  SIGN  ++ ++T V   S      +W+  +N+N G  +Y+YWL++ 
Sbjct: 492 SMRSTATALFWMAISIGNYVSTLLVTLVHKFSAKPDGSNWLPDNNLNRGRLEYFYWLITV 551

Query: 554 LLFANFLYYLACSKSY 561
           L   N +YYL C+K Y
Sbjct: 552 LQAVNLVYYLWCAKIY 565

BLAST of Cucsa.161360 vs. NCBI nr
Match: gi|778672069|ref|XP_011649736.1| (PREDICTED: protein NRT1/ PTR FAMILY 1.2 [Cucumis sativus])

HSP 1 Score: 1151.3 bits (2977), Expect = 0.0e+00
Identity = 578/581 (99.48%), Postives = 579/581 (99.66%), Query Frame = 1

Query: 1   MEDQLVVDENRGMEEPLLSDTDNRKGGFRTLPFIIANGALEKLASQGLSPSMILYLTKVY 60
           MEDQLVVDENRGMEEPLLSDTDNRKGGFRTLPFIIANGALEKLASQGLSPSMILYLTKVY
Sbjct: 1   MEDQLVVDENRGMEEPLLSDTDNRKGGFRTLPFIIANGALEKLASQGLSPSMILYLTKVY 60

Query: 61  GMKSAHASNVIFLWSAASNFTPIICAFLADSYFGRFPMIAAGSIFSFLGMFVLWLTAMIP 120
           GMKSAH+SNVIFLWSAASNFTPIICAFLADSYFGRFPMIAAGSIFSFLGMFVLWLTAMIP
Sbjct: 61  GMKSAHSSNVIFLWSAASNFTPIICAFLADSYFGRFPMIAAGSIFSFLGMFVLWLTAMIP 120

Query: 121 QARPFCDEISGHCDAPSTSQLLLLYSSYAIMSVGSGCLQASYLAFGADQLYRKNKSNSGI 180
           QARPFCDEISGHCDAPSTSQLLLLYSSYAIMSVGSGCLQASYLAFGADQLYRKNKSNSGI
Sbjct: 121 QARPFCDEISGHCDAPSTSQLLLLYSSYAIMSVGSGCLQASYLAFGADQLYRKNKSNSGI 180

Query: 181 LDSYFNVCYISAALGTLVGMSCIVYIQDRMGWGMGFGVPVALMFLATITFLSASPLYLKS 240
           LDSYFNVCYISAALGTLVGMSCIVYIQDRMGWGMGFGVPVALMFLATITFLSASPLYLKS
Sbjct: 181 LDSYFNVCYISAALGTLVGMSCIVYIQDRMGWGMGFGVPVALMFLATITFLSASPLYLKS 240

Query: 241 KPSKSWCAGLVQVVFAAYKKRHTQIPFVGTSEMYHHENESPCTLPSNKLRFLNKACIIRN 300
           KPSKSWCAGLVQVVFAAYKKRHTQIPFVGTSEMYHHENESPCTLPSNKLRFLNKACIIRN
Sbjct: 241 KPSKSWCAGLVQVVFAAYKKRHTQIPFVGTSEMYHHENESPCTLPSNKLRFLNKACIIRN 300

Query: 301 SKEELTLDGKASNPWSLCTVEQVENLKALIRIIPLWSTGILVSASLSQSFYVLQVASMNR 360
           SKEELTLDGKASNPWSLCTVEQVENLKALIRIIPLWSTGILVSASLSQSFYVLQVASMNR
Sbjct: 301 SKEELTLDGKASNPWSLCTVEQVENLKALIRIIPLWSTGILVSASLSQSFYVLQVASMNR 360

Query: 361 HLTSSFEVPAGSFSAIIVVSLIIWIALYDRLILPLASKCRGKPTRLCAKTRMGMGILCCC 420
           HLTSSFEVPAGSFSAIIVVSLIIWIALYDRLILPLASKCRGKPTRLCAKTRMGMGILCC 
Sbjct: 361 HLTSSFEVPAGSFSAIIVVSLIIWIALYDRLILPLASKCRGKPTRLCAKTRMGMGILCCG 420

Query: 421 LSLAVSAIVEGDRRALAIKEGFSDNPNAVVSMSAFWTLPRYILFGIAEALNAIGQIEFFY 480
           LSLAVSAIVEGDRRALAIKEGFSDNPNAVVSMSAFWTLPRYILFGIAEALNAIGQIEFFY
Sbjct: 421 LSLAVSAIVEGDRRALAIKEGFSDNPNAVVSMSAFWTLPRYILFGIAEALNAIGQIEFFY 480

Query: 481 NELPKAMSSVATSLLGLNSSIGNLAASFIMTTVDNLSKSVGVKSWVSSNINEGHSDYYYW 540
           NELPKAMSSVATSLLGLNSSIGNLAASFIMTTVDNLSKSVGVKSWVSSNINEGHSDYYYW
Sbjct: 481 NELPKAMSSVATSLLGLNSSIGNLAASFIMTTVDNLSKSVGVKSWVSSNINEGHSDYYYW 540

Query: 541 LLSGLLFANFLYYLACSKSYGPSNEESVGRANAEDYKNTVN 582
           LLSGLLFANFLYYLACSKSYGPSNEESVGRANAEDYKNT N
Sbjct: 541 LLSGLLFANFLYYLACSKSYGPSNEESVGRANAEDYKNTAN 581

BLAST of Cucsa.161360 vs. NCBI nr
Match: gi|659088518|ref|XP_008445024.1| (PREDICTED: protein NRT1/ PTR FAMILY 1.2-like [Cucumis melo])

HSP 1 Score: 1042.7 bits (2695), Expect = 2.4e-301
Identity = 520/581 (89.50%), Postives = 546/581 (93.98%), Query Frame = 1

Query: 1   MEDQLVVDENRGMEEPLLSDTDNRKGGFRTLPFIIANGALEKLASQGLSPSMILYLTKVY 60
           MED LVVDENRGMEEPLLSDTD+ KGG RTLPFIIANGALEKLASQGLSPSMILYLT+VY
Sbjct: 1   MEDPLVVDENRGMEEPLLSDTDDGKGGVRTLPFIIANGALEKLASQGLSPSMILYLTQVY 60

Query: 61  GMKSAHASNVIFLWSAASNFTPIICAFLADSYFGRFPMIAAGSIFSFLGMFVLWLTAMIP 120
           GMKSA ASNVIFLWSAASNFTPIICAFLADSYFGRFPM+AAGSIFSFLGMFVLWLT MIP
Sbjct: 61  GMKSAQASNVIFLWSAASNFTPIICAFLADSYFGRFPMVAAGSIFSFLGMFVLWLTVMIP 120

Query: 121 QARPFCDEISGHCDAPSTSQLLLLYSSYAIMSVGSGCLQASYLAFGADQLYRKNKSNSGI 180
           QARP CDEI+GHC+APS  QLLLLYSSYAIMS+GSGCLQASYLAFGADQLYRKNKS SGI
Sbjct: 121 QARPLCDEINGHCNAPSIPQLLLLYSSYAIMSIGSGCLQASYLAFGADQLYRKNKSKSGI 180

Query: 181 LDSYFNVCYISAALGTLVGMSCIVYIQDRMGWGMGFGVPVALMFLATITFLSASPLYLKS 240
           LD+YFN+CYISAALGTLVGMSCIVYIQDRMGWGMGFGVPV LM LATITFLSAS LYLKS
Sbjct: 181 LDNYFNICYISAALGTLVGMSCIVYIQDRMGWGMGFGVPVVLMLLATITFLSASSLYLKS 240

Query: 241 KPSKSWCAGLVQVVFAAYKKRHTQIPFVGTSEMYHHENESPCTLPSNKLRFLNKACIIRN 300
            PSKSWCAGLVQVVFAAYKKRH QI FVGTS MYHHEN S C LPS+KLRFLNKACIIRN
Sbjct: 241 MPSKSWCAGLVQVVFAAYKKRHMQIAFVGTSGMYHHENGSLCALPSDKLRFLNKACIIRN 300

Query: 301 SKEELTLDGKASNPWSLCTVEQVENLKALIRIIPLWSTGILVSASLSQSFYVLQVASMNR 360
           S+EELT DGKASNPWSLCTVEQVENLKALI+IIPLWSTGILVSASL+QSFYVLQ+ASM+R
Sbjct: 301 SEEELTSDGKASNPWSLCTVEQVENLKALIKIIPLWSTGILVSASLNQSFYVLQLASMDR 360

Query: 361 HLTSSFEVPAGSFSAIIVVSLIIWIALYDRLILPLASKCRGKPTRLCAKTRMGMGILCCC 420
           HLTSSFEVPAGSF A++VVSLIIWI LYDRLILPLASKCRGKPTRL AKTRMG+GILCC 
Sbjct: 361 HLTSSFEVPAGSFGAMLVVSLIIWITLYDRLILPLASKCRGKPTRLSAKTRMGLGILCCT 420

Query: 421 LSLAVSAIVEGDRRALAIKEGFSDNPNAVVSMSAFWTLPRYILFGIAEALNAIGQIEFFY 480
           LSLAVSA+VEG RRALAI+EGFSD+PNAVVSMSAFWTLPRYILFG+AEA NAIGQIEFFY
Sbjct: 421 LSLAVSAVVEGYRRALAIREGFSDDPNAVVSMSAFWTLPRYILFGMAEAFNAIGQIEFFY 480

Query: 481 NELPKAMSSVATSLLGLNSSIGNLAASFIMTTVDNLSKSVGVKSWVSSNINEGHSDYYYW 540
           NELPKAMSSVATSLLGLNSS+GNLAASFIMTTVDN SK+VG KSWVSSNIN+GHSDYYYW
Sbjct: 481 NELPKAMSSVATSLLGLNSSVGNLAASFIMTTVDNFSKAVGGKSWVSSNINDGHSDYYYW 540

Query: 541 LLSGLLFANFLYYLACSKSYGPSNEESVGRANAEDYKNTVN 582
           LL GLLFAN LY+LACSKSYGPS EES GR+ AEDY N VN
Sbjct: 541 LLFGLLFANLLYFLACSKSYGPSKEESGGRSTAEDYNNAVN 581

BLAST of Cucsa.161360 vs. NCBI nr
Match: gi|659088522|ref|XP_008445026.1| (PREDICTED: protein NRT1/ PTR FAMILY 1.2-like [Cucumis melo])

HSP 1 Score: 1022.3 bits (2642), Expect = 3.3e-295
Identity = 508/581 (87.44%), Postives = 540/581 (92.94%), Query Frame = 1

Query: 1   MEDQLVVDENRGMEEPLLSDTDNRKGGFRTLPFIIANGALEKLASQGLSPSMILYLTKVY 60
           MEDQLVVDENRGMEEPLLSDTD+ KGG RTLPFIIANG  EK+AS GLSPSMILYLT+VY
Sbjct: 1   MEDQLVVDENRGMEEPLLSDTDDGKGGLRTLPFIIANGTFEKVASHGLSPSMILYLTEVY 60

Query: 61  GMKSAHASNVIFLWSAASNFTPIICAFLADSYFGRFPMIAAGSIFSFLGMFVLWLTAMIP 120
           GMKSAHASNVIFLWSAA+NFTPIICAFLADSYFGRF MIAAGSIFSFLGMFVLWLTA+IP
Sbjct: 61  GMKSAHASNVIFLWSAATNFTPIICAFLADSYFGRFSMIAAGSIFSFLGMFVLWLTAIIP 120

Query: 121 QARPFCDEISGHCDAPSTSQLLLLYSSYAIMSVGSGCLQASYLAFGADQLYRKNKSNSGI 180
           Q RPFCD+I+G+CDAPST QLLLLYSSYAIMS+GSGCLQAS+LAFGADQLY+++KSNSGI
Sbjct: 121 QVRPFCDKINGYCDAPSTPQLLLLYSSYAIMSIGSGCLQASHLAFGADQLYKESKSNSGI 180

Query: 181 LDSYFNVCYISAALGTLVGMSCIVYIQDRMGWGMGFGVPVALMFLATITFLSASPLYLKS 240
           L+SYFN+CYIS A+GTL+GMSCIVYIQDRMGWG+GFGV VALM LATITFLSAS LYLKS
Sbjct: 181 LNSYFNICYISIAVGTLIGMSCIVYIQDRMGWGIGFGVSVALMLLATITFLSASSLYLKS 240

Query: 241 KPSKSWCAGLVQVVFAAYKKRHTQIPFVGTSEMYHHENESPCTLPSNKLRFLNKACIIRN 300
            PSKSWC GLVQVVFA YKKRH QIPFVGT EMYHHE  S C LPSNKLRFLNKACIIRN
Sbjct: 241 MPSKSWCDGLVQVVFATYKKRHMQIPFVGTFEMYHHETGSLCALPSNKLRFLNKACIIRN 300

Query: 301 SKEELTLDGKASNPWSLCTVEQVENLKALIRIIPLWSTGILVSASLSQSFYVLQVASMNR 360
            +EELT DGKASNPWSLCTVEQVENLKALIRIIPLWSTGIL S SLSQSFYVLQVASM+R
Sbjct: 301 FEEELTSDGKASNPWSLCTVEQVENLKALIRIIPLWSTGILFSVSLSQSFYVLQVASMDR 360

Query: 361 HLTSSFEVPAGSFSAIIVVSLIIWIALYDRLILPLASKCRGKPTRLCAKTRMGMGILCCC 420
           HLTSSFEVPAGSFS +IVV +IIWI LY+ LILPLASKCRGKPT L  KTRMG+GILCC 
Sbjct: 361 HLTSSFEVPAGSFSVLIVVFVIIWIILYNCLILPLASKCRGKPTHLSGKTRMGLGILCCT 420

Query: 421 LSLAVSAIVEGDRRALAIKEGFSDNPNAVVSMSAFWTLPRYILFGIAEALNAIGQIEFFY 480
           LSLAVSAIVEGDRRALAIKEGFS++PNAVVSMSAFWTLPRYILFG+AEA NAIGQIEFFY
Sbjct: 421 LSLAVSAIVEGDRRALAIKEGFSNDPNAVVSMSAFWTLPRYILFGMAEAFNAIGQIEFFY 480

Query: 481 NELPKAMSSVATSLLGLNSSIGNLAASFIMTTVDNLSKSVGVKSWVSSNINEGHSDYYYW 540
           NELPKAMSSVATSLLGLNSS+GNLAASFIMTTVDN SK+VG KSWVSSNIN+GHSDYYYW
Sbjct: 481 NELPKAMSSVATSLLGLNSSVGNLAASFIMTTVDNFSKAVGGKSWVSSNINDGHSDYYYW 540

Query: 541 LLSGLLFANFLYYLACSKSYGPSNEESVGRANAEDYKNTVN 582
           LL GLLFANFLY+LACSKSYGPS EES GR+ AEDY NTVN
Sbjct: 541 LLFGLLFANFLYFLACSKSYGPSKEESAGRSTAEDYNNTVN 581

BLAST of Cucsa.161360 vs. NCBI nr
Match: gi|778672065|ref|XP_011649735.1| (PREDICTED: protein NRT1/ PTR FAMILY 1.2-like [Cucumis sativus])

HSP 1 Score: 976.9 bits (2524), Expect = 1.6e-281
Identity = 492/581 (84.68%), Postives = 525/581 (90.36%), Query Frame = 1

Query: 1   MEDQLVVDENRGMEEPLLSDTDNRKGGFRTLPFIIANGALEKLASQGLSPSMILYLTKVY 60
           MED LVV ENRGMEEPLLS TD+ KGG RTLPFIIA+GALEKLAS GLSPSMILYLT+VY
Sbjct: 1   MEDPLVVGENRGMEEPLLSGTDDCKGGIRTLPFIIASGALEKLASMGLSPSMILYLTEVY 60

Query: 61  GMKSAHASNVIFLWSAASNFTPIICAFLADSYFGRFPMIAAGSIFSFLGMFVLWLTAMIP 120
           GMKS  AS+VIFLW+AA+ FTPIICAFLADSYFGRF MI AGSIFS LGMFVLWLTAMIP
Sbjct: 61  GMKSVQASSVIFLWTAATYFTPIICAFLADSYFGRFMMIVAGSIFSCLGMFVLWLTAMIP 120

Query: 121 QARPFCDEISGHCDAPSTSQLLLLYSSYAIMSVGSGCLQASYLAFGADQLYRKNKSNSGI 180
           QARPFCDEISGHC+APST QLL LYSSYAIMS+GSGCLQ+S  AFGADQLY++NKSNSGI
Sbjct: 121 QARPFCDEISGHCNAPSTPQLLFLYSSYAIMSIGSGCLQSSNFAFGADQLYKENKSNSGI 180

Query: 181 LDSYFNVCYISAALGTLVGMSCIVYIQDRMGWGMGFGVPVALMFLATITFLSASPLYLKS 240
           LD YFN+CYIS ++GTLVGMSCIVYIQDRMGW MGFGVP+ALM LAT+TFLSAS LYLKS
Sbjct: 181 LDIYFNLCYISVSVGTLVGMSCIVYIQDRMGWEMGFGVPIALMLLATVTFLSASSLYLKS 240

Query: 241 KPSKSWCAGLVQVVFAAYKKRHTQIPFVGTSEMYHHENESPCTLPSNKLRFLNKACIIRN 300
            PSKSWCAGLV VVFAAYKKR  QIPFVGTSEMYHHEN S CTLPS+KLRFLNKACII+N
Sbjct: 241 VPSKSWCAGLVHVVFAAYKKRRMQIPFVGTSEMYHHENGSSCTLPSDKLRFLNKACIIKN 300

Query: 301 SKEELTLDGKASNPWSLCTVEQVENLKALIRIIPLWSTGILVSASLSQSFYVLQVASMNR 360
            +EEL  DGKASNPWSLCTVEQVENLKALIRIIPLWSTGILVSAS+SQS YVLQVASM+R
Sbjct: 301 YEEELISDGKASNPWSLCTVEQVENLKALIRIIPLWSTGILVSASMSQSVYVLQVASMDR 360

Query: 361 HLTSSFEVPAGSFSAIIVVSLIIWIALYDRLILPLASKCRGKPTRLCAKTRMGMGILCCC 420
           HLTSSFEVPAGSF A++VV +IIWI LYDRLILPLAS+ RGKPTRL  KTRMGM I  C 
Sbjct: 361 HLTSSFEVPAGSFIAMLVVFVIIWIILYDRLILPLASRYRGKPTRLSGKTRMGMAIPFCI 420

Query: 421 LSLAVSAIVEGDRRALAIKEGFSDNPNAVVSMSAFWTLPRYILFGIAEALNAIGQIEFFY 480
           LSLAVSAIVEG RRALAIKEGFSD+PNAVVSMSAFWTLPRYILFGIAEA  AIGQ+EFFY
Sbjct: 421 LSLAVSAIVEGYRRALAIKEGFSDDPNAVVSMSAFWTLPRYILFGIAEAFYAIGQLEFFY 480

Query: 481 NELPKAMSSVATSLLGLNSSIGNLAASFIMTTVDNLSKSVGVKSWVSSNINEGHSDYYYW 540
           NELPKAMSSVATSLLGL+S  GNLAASFIMTTVDN SK++GVKSWVSSNINEGH+DYYYW
Sbjct: 481 NELPKAMSSVATSLLGLSSCAGNLAASFIMTTVDNFSKTIGVKSWVSSNINEGHNDYYYW 540

Query: 541 LLSGLLFANFLYYLACSKSYGPSNEESVGRANAEDYKNTVN 582
           LL GLL ANF YYLAC+ SYGPS EES   +NAEDY NTVN
Sbjct: 541 LLFGLLVANFFYYLACNNSYGPSKEESEDISNAEDYNNTVN 581

BLAST of Cucsa.161360 vs. NCBI nr
Match: gi|659088520|ref|XP_008445025.1| (PREDICTED: protein NRT1/ PTR FAMILY 1.2-like [Cucumis melo])

HSP 1 Score: 946.0 bits (2444), Expect = 3.0e-272
Identity = 467/532 (87.78%), Postives = 498/532 (93.61%), Query Frame = 1

Query: 50  PSMILYLTKVYGMKSAHASNVIFLWSAASNFTPIICAFLADSYFGRFPMIAAGSIFSFLG 109
           PSMILYLT+VYGMKSA ASNVIFLWSAA+NFTPI+CAFLADSYFGRFPM+AAGSIFSFLG
Sbjct: 2   PSMILYLTEVYGMKSAQASNVIFLWSAATNFTPIVCAFLADSYFGRFPMVAAGSIFSFLG 61

Query: 110 MFVLWLTAMIPQARPFCDEISGHCDAPSTSQLLLLYSSYAIMSVGSGCLQASYLAFGADQ 169
             VLWLTAMIPQARPFCDEISG+CDAPST QLLLLYSSYAI+S+GSGC+QASY+AFGADQ
Sbjct: 62  TIVLWLTAMIPQARPFCDEISGYCDAPSTPQLLLLYSSYAIISIGSGCVQASYIAFGADQ 121

Query: 170 LYRKNKSNSGILDSYFNVCYISAALGTLVGMSCIVYIQDRMGWGMGFGVPVALMFLATIT 229
           LYRKNKSNSGILDSYFN CYISAALGTLVGMSCIVYIQDR+GWGMGFGVPVALM LATIT
Sbjct: 122 LYRKNKSNSGILDSYFNTCYISAALGTLVGMSCIVYIQDRIGWGMGFGVPVALMLLATIT 181

Query: 230 FLSASPLYLKSKPSKSWCAGLVQVVFAAYKKRHTQIPFVGTSEMYHHENESPCTLPSNKL 289
           F  ASPLYLKS PS+SWCAGLVQVVFAAYKKRH QIPFVGTSE+YH EN S C LPS+KL
Sbjct: 182 FFLASPLYLKSMPSESWCAGLVQVVFAAYKKRHMQIPFVGTSEIYHQENGSSCALPSDKL 241

Query: 290 RFLNKACIIRNSKEELTLDGKASNPWSLCTVEQVENLKALIRIIPLWSTGILVSASLSQS 349
           RF NKACIIRNS+EELT DGKASNPWSL TVEQVENLKAL+RIIPLWSTGILVSASL+QS
Sbjct: 242 RFFNKACIIRNSEEELTPDGKASNPWSLSTVEQVENLKALMRIIPLWSTGILVSASLNQS 301

Query: 350 FYVLQVASMNRHLTSSFEVPAGSFSAIIVVSLIIWIALYDRLILPLASKCRGKPTRLCAK 409
           FYVLQVASM+RHLTSSFEVPAGSFS +++V LIIWI LYDRLILPLASKCRGKPTRL AK
Sbjct: 302 FYVLQVASMDRHLTSSFEVPAGSFSMMVLVLLIIWITLYDRLILPLASKCRGKPTRLSAK 361

Query: 410 TRMGMGILCCCLSLAVSAIVEGDRRALAIKEGFSDNPNAVVSMSAFWTLPRYILFGIAEA 469
           TR+G+GILCC LSLAVSAIVE DRRALAIKEGFSDNPNAVVSMSAFWTLPRYIL GI E 
Sbjct: 362 TRIGIGILCCTLSLAVSAIVESDRRALAIKEGFSDNPNAVVSMSAFWTLPRYILLGIEEG 421

Query: 470 LNAIGQIEFFYNELPKAMSSVATSLLGLNSSIGNLAASFIMTTVDNLSKSVGVKSWVSSN 529
            + I Q+EFFYNELPKAMSSVATSLLGLNSS+GNLAASFIMTTVDNLS++VG +SWVSSN
Sbjct: 422 FSVIAQVEFFYNELPKAMSSVATSLLGLNSSVGNLAASFIMTTVDNLSEAVGGRSWVSSN 481

Query: 530 INEGHSDYYYWLLSGLLFANFLYYLACSKSYGPSNEESVGRANAEDYKNTVN 582
           INEGHSDYYYWLL GLLFANFLY+LACSKSYGPS EES GR++AEDYKN VN
Sbjct: 482 INEGHSDYYYWLLFGLLFANFLYFLACSKSYGPSKEESAGRSSAEDYKNIVN 533