Cucsa.158780 (gene) Cucumber (Gy14) v1

Overview
NameCucsa.158780
Typegene
OrganismCucumis sativus (Cucumber (Gy14) v1)
DescriptionMetalloendoproteinase 1, putative
Locationscaffold01142 : 860699 .. 861579 (-)
Genome Viewer
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDS
Hold the cursor over a type above to highlight its positions in the sequence below.
CTTTTCCTTTTATTTGGGCACGTGATCTTCATGATATCAAAAAATCCCAACTTCTATTTCCTCAACATCTTCTAGGAAGTAGTAAAGGTCACAACATAGAAGGAATCCATACCATCAAAATGCACCTCCAACGTTATGGTTACTTAAGCAAAAACTATAACATTATCGATACGAACGGTGCTTATAATAACGCCTTCGACGACCACCTAGAATCCGCCATAAAAAAATATCAAATGTTCTTCAAGCTTCCCAAGAGTGGAGTCTTAGACATGGAGACATTACACCAAATGTCACAAGCACGATGTTCGGTTCCCGACATATTCGAGAACAACGAGAACGAGACGAGCGTGACAACAAGTAATCTCCATATAGGAAGTAAGTACACATTTTTTCCAGGGAGAGTGAAATGGCCAGATTCTCTTAATTACCGACTAACATACGCACTCGTTAACAATTTCCCGGAAGAGTTTAAGGAGTCGGTGAGAACGGCATTTGAGATATGGTACGGACGCAGTCGCTTCAATTTCACAGAAGTCAGTGAGAACGAAGGGGGGAATATAAGAATAAGCTTCGAGAGAGGAGTCCATGGCGATTATCATCCTTTCACTAAGAATTCAAAAACTTTGGCACATACGTTTGCACCAATCGATGGGAGATTTCACTTCAATGCTGATAAACCTTTTTCAGTTGATGTTACATATAATGCGTATCATTTGAGGACTGTGGCATTGCATGAGCTTGGACATGCATTTGGGTTGGCTCATAGCCCAAGTGAAGATGCCATTATGTTTCCCACTATACCTACTAATCTTGAAAAGGATTTAGATATGGATGATATTGAAGGATTGTGGGAATTATATGATGGATTTGGTGTTGCTTAA

mRNA sequence

CTTTTCCTTTTATTTGGGCACGTGATCTTCATGATATCAAAAAATCCCAACTTCTATTTCCTCAACATCTTCTAGGAAGTAGTAAAGGTCACAACATAGAAGGAATCCATACCATCAAAATGCACCTCCAACGTTATGGTTACTTAAGCAAAAACTATAACATTATCGATACGAACGGTGCTTATAATAACGCCTTCGACGACCACCTAGAATCCGCCATAAAAAAATATCAAATGTTCTTCAAGCTTCCCAAGAGTGGAGTCTTAGACATGGAGACATTACACCAAATGTCACAAGCACGATGTTCGGTTCCCGACATATTCGAGAACAACGAGAACGAGACGAGCGTGACAACAAGTAATCTCCATATAGGAAGTAAGTACACATTTTTTCCAGGGAGAGTGAAATGGCCAGATTCTCTTAATTACCGACTAACATACGCACTCGTTAACAATTTCCCGGAAGAGTTTAAGGAGTCGGTGAGAACGGCATTTGAGATATGGTACGGACGCAGTCGCTTCAATTTCACAGAAGTCAGTGAGAACGAAGGGGGGAATATAAGAATAAGCTTCGAGAGAGGAGTCCATGGCGATTATCATCCTTTCACTAAGAATTCAAAAACTTTGGCACATACGTTTGCACCAATCGATGGGAGATTTCACTTCAATGCTGATAAACCTTTTTCAGTTGATGTTACATATAATGCGTATCATTTGAGGACTGTGGCATTGCATGAGCTTGGACATGCATTTGGGTTGGCTCATAGCCCAAGTGAAGATGCCATTATGTTTCCCACTATACCTACTAATCTTGAAAAGGATTTAGATATGGATGATATTGAAGGATTGTGGGAATTATATGATGGATTTGGTGTTGCTTAA

Coding sequence (CDS)

CTTTTCCTTTTATTTGGGCACGTGATCTTCATGATATCAAAAAATCCCAACTTCTATTTCCTCAACATCTTCTAGGAAGTAGTAAAGGTCACAACATAGAAGGAATCCATACCATCAAAATGCACCTCCAACGTTATGGTTACTTAAGCAAAAACTATAACATTATCGATACGAACGGTGCTTATAATAACGCCTTCGACGACCACCTAGAATCCGCCATAAAAAAATATCAAATGTTCTTCAAGCTTCCCAAGAGTGGAGTCTTAGACATGGAGACATTACACCAAATGTCACAAGCACGATGTTCGGTTCCCGACATATTCGAGAACAACGAGAACGAGACGAGCGTGACAACAAGTAATCTCCATATAGGAAGTAAGTACACATTTTTTCCAGGGAGAGTGAAATGGCCAGATTCTCTTAATTACCGACTAACATACGCACTCGTTAACAATTTCCCGGAAGAGTTTAAGGAGTCGGTGAGAACGGCATTTGAGATATGGTACGGACGCAGTCGCTTCAATTTCACAGAAGTCAGTGAGAACGAAGGGGGGAATATAAGAATAAGCTTCGAGAGAGGAGTCCATGGCGATTATCATCCTTTCACTAAGAATTCAAAAACTTTGGCACATACGTTTGCACCAATCGATGGGAGATTTCACTTCAATGCTGATAAACCTTTTTCAGTTGATGTTACATATAATGCGTATCATTTGAGGACTGTGGCATTGCATGAGCTTGGACATGCATTTGGGTTGGCTCATAGCCCAAGTGAAGATGCCATTATGTTTCCCACTATACCTACTAATCTTGAAAAGGATTTAGATATGGATGATATTGAAGGATTGTGGGAATTATATGATGGATTTGGTGTTGCTTAA

Protein sequence

FPFIWARDLHDIKKSQLLFPQHLLGSSKGHNIEGIHTIKMHLQRYGYLSKNYNIIDTNGAYNNAFDDHLESAIKKYQMFFKLPKSGVLDMETLHQMSQARCSVPDIFENNENETSVTTSNLHIGSKYTFFPGRVKWPDSLNYRLTYALVNNFPEEFKESVRTAFEIWYGRSRFNFTEVSENEGGNIRISFERGVHGDYHPFTKNSKTLAHTFAPIDGRFHFNADKPFSVDVTYNAYHLRTVALHELGHAFGLAHSPSEDAIMFPTIPTNLEKDLDMDDIEGLWELYDGFGVA*
Homology
[Close]
BLAST of Cucsa.158780 vs. Swiss-Prot
Match: 2MMP_ARATH (Metalloendoproteinase 2-MMP OS=Arabidopsis thaliana GN=2MMP PE=1 SV=1)

HSP 1 Score: 165.2 bits (417), Expect = 1.0e-39
Identity = 100/281 (35.59%), Postives = 145/281 (51.60%), Query Frame = 1 

Query: 25  GSSKGHNIEGIHTIKMHLQRYGYLSKNYNIIDTNGAYNNAFDDHLESAIKKYQMFFKLPK 84
           G   G N++G++ IK + QR+GY+ + ++     G + + FDD L++A++ YQ  F L  
Sbjct: 49  GCHHGQNVDGLYRIKKYFQRFGYIPETFS-----GNFTDDFDDILKAAVELYQTNFNLNV 108

Query: 85  SGVLDMETLHQMSQARCSVPDIFENNEN---------ETSVTTSNLHIGSKYTFFPGRVK 144
           +G LD  T+  +   RC  PD+               E + + ++LH   +YT FPG  +
Sbjct: 109 TGELDALTIQHIVIPRCGNPDVVNGTSLMHGGRRKTFEVNFSRTHLHAVKRYTLFPGEPR 168

Query: 145 WPDSLNYRLTYAL--VNNFPEEFKESVRTAFEIWYGRSRFNFTEVSENEGGNIRISFERG 204
           WP +    LTYA    N   EE K     AF  W   +  NFT        +I I F  G
Sbjct: 169 WPRNRR-DLTYAFDPKNPLTEEVKSVFSRAFGRWSDVTALNFTLSESFSTSDITIGFYTG 228

Query: 205 VHGDYHPFTKNSKTLAHTFAPIDGRFHFNADKPFSVDVTYNAY-------HLRTVALHEL 264
            HGD  PF     TLAH F+P  G+FH +AD+ + V    +++        L +VA+HE+
Sbjct: 229 DHGDGEPFDGVLGTLAHAFSPPSGKFHLDADENWVVSGDLDSFLSVTAAVDLESVAVHEI 288

Query: 265 GHAFGLAHSPSEDAIMFPTIPTNLEK-DLDMDDIEGLWELY 287
           GH  GL HS  E++IM+PTI T   K DL  DD+EG+  LY
Sbjct: 289 GHLLGLGHSSVEESIMYPTITTGKRKVDLTNDDVEGIQYLY 323

[Close]
BLAST of Cucsa.158780 vs. Swiss-Prot
Match: 5MMP_ARATH (Metalloendoproteinase 5-MMP OS=Arabidopsis thaliana GN=5MMP PE=1 SV=1)

HSP 1 Score: 156.8 bits (395), Expect = 3.7e-37
Identity = 102/278 (36.69%), Postives = 132/278 (47.48%), Query Frame = 1 

Query: 23  LLGSSKGHNIEGIHTIKMHLQRYGYLSKNYNIIDTNGAYNNAFDDHLESAIKKYQMFFKL 82
           L G   G NI G+  +K + +R+GY++       T G   + FDD L+SAI  YQ  F L
Sbjct: 48  LAGCHIGENINGLSKLKQYFRRFGYIT-------TTGNCTDDFDDVLQSAINTYQKNFNL 107

Query: 83  PKSGVLDMETLHQMSQARCSVPDIFENNENETSVTTSNLHIGSKYTFFPGRVKWPDSLNY 142
             +G LD  TL Q+ + RC  PD+ +            L    KY+FFPG+ +WP     
Sbjct: 108 KVTGKLDSSTLRQIVKPRCGNPDLIDGVSEMNG--GKILRTTEKYSFFPGKPRWP-KRKR 167

Query: 143 RLTYALV--NNFPEEFKESVRTAFEIWYGRSRFNFTEVSENEGGNIRISFERGVHGDYHP 202
            LTYA    NN  +E K     AF  W   +  NFT        +I I F  G HGD  P
Sbjct: 168 DLTYAFAPQNNLTDEVKRVFSRAFTRWAEVTPLNFTRSESILRADIVIGFFSGEHGDGEP 227

Query: 203 FTKNSKTLAHTFAPIDGRFHFNADKPFSVD---------VTYNAYHLRTVALHELGHAFG 262
           F     TLAH  +P  G  H + D+ + +                 L +VA+HE+GH  G
Sbjct: 228 FDGAMGTLAHASSPPTGMLHLDGDEDWLISNGEISRRILPVTTVVDLESVAVHEIGHLLG 287

Query: 263 LAHSPSEDAIMFPTIPTNLEK-DLDMDDIEGLWELYDG 289
           L HS  EDAIMFP I     K +L  DDIEG+  LY G
Sbjct: 288 LGHSSVEDAIMFPAISGGDRKVELAKDDIEGIQHLYGG 315

[Close]
BLAST of Cucsa.158780 vs. Swiss-Prot
Match: MEP1_SOYBN (Metalloendoproteinase 1 OS=Glycine max PE=1 SV=2)

HSP 1 Score: 154.1 bits (388), Expect = 2.4e-36
Identity = 101/267 (37.83%), Postives = 134/267 (50.19%), Query Frame = 1 

Query: 29  GHNIEGIHTIKMHLQRYGYLSKNYNIIDTNGAYNNAFDDHLESAIKKYQMFFKLPKSGVL 88
           G N +G+  +K +    GY+    +  D        FDD L SAIK YQ  + L  +G  
Sbjct: 48  GQNYKGLSNVKNYFHHLGYIPNAPHFDDN-------FDDTLVSAIKTYQKNYNLNVTGKF 107

Query: 89  DMETLHQMSQARCSVPDIFENNENETSVTTSNLHIGSKYTFFPGRVKWPDSLNYRLTYAL 148
           D+ TL Q+   RC VPDI  N    TS       + S YTFF    +W      +LTYA 
Sbjct: 108 DINTLKQIMTPRCGVPDIIINTNKTTS-----FGMISDYTFFKDMPRWQAGTT-QLTYAF 167

Query: 149 VNN--FPEEFKESVRTAFEIWYGRSRFNFTEVSENEGGNIRISFERGVHGDYHPFTKNSK 208
                  + FK ++  AF  W       F E +  E  NI+I F    HGD +PF     
Sbjct: 168 SPEPRLDDTFKSAIARAFSKWTPVVNIAFQETTSYETANIKILFASKNHGDPYPFDGPGG 227

Query: 209 TLAHTFAPIDGRFHFNADKPF--SVDVT----YNAYHLRTVALHELGHAFGLAHSPSEDA 268
            L H FAP DGR HF+AD+ +  S DVT     +A+ L +VA+HE+GH  GL HS    A
Sbjct: 228 ILGHAFAPTDGRCHFDADEYWVASGDVTKSPVTSAFDLESVAVHEIGHLLGLGHSSDLRA 287

Query: 269 IMFPTIPTNLEK-DLDMDDIEGLWELY 287
           IM+P+IP    K +L  DDI+G+ +LY
Sbjct: 288 IMYPSIPPRTRKVNLAQDDIDGIRKLY 301

[Close]
BLAST of Cucsa.158780 vs. Swiss-Prot
Match: 3MMP_ARATH (Metalloendoproteinase 3-MMP OS=Arabidopsis thaliana GN=3MMP PE=1 SV=1)

HSP 1 Score: 153.3 bits (386), Expect = 4.1e-36
Identity = 100/291 (34.36%), Postives = 140/291 (48.11%), Query Frame = 1 

Query: 25  GSSKGHNIEGIHTIKMHLQRYGYLSKNYNIIDTNGAYNNAFDDHLESAIKKYQMFFKLPK 84
           G   G   +G++ +K + Q +GY+++     + +G + + FDD L++A++ YQ  F+L  
Sbjct: 51  GCHAGKKYDGLYMLKQYFQHFGYITET----NLSGNFTDDFDDILKNAVEMYQRNFQLNV 110

Query: 85  SGVLDMETLHQMSQARCSVPDIFENNENETSVTTSNLHIGSK------------------ 144
           +GVLD  TL  +   RC  PD+           TS +H G K                  
Sbjct: 111 TGVLDELTLKHVVIPRCGNPDVVNG--------TSTMHSGRKTFEVSFAGRGQRFHAVKH 170

Query: 145 YTFFPGRVKWPDSLNYRLTYALV--NNFPEEFKESVRTAFEIWYGRSRFNFTEVSENEGG 204
           Y+FFPG  +WP +    LTYA    N   EE K     AF  W   +   FT V      
Sbjct: 171 YSFFPGEPRWPRNRR-DLTYAFDPRNALTEEVKSVFSRAFTRWEEVTPLTFTRVERFSTS 230

Query: 205 NIRISFERGVHGDYHPFTKNSKTLAHTFAPIDGRFHFNADKPFSVD--------VTYNAY 264
           +I I F  G HGD  PF    +TLAH F+P  G FH + ++ + V             A 
Sbjct: 231 DISIGFYSGEHGDGEPFDGPMRTLAHAFSPPTGHFHLDGEENWIVSGEGGDGFISVSEAV 290

Query: 265 HLRTVALHELGHAFGLAHSPSEDAIMFPTIPTNLEK-DLDMDDIEGLWELY 287
            L +VA+HE+GH  GL HS  E +IM+PTI T   K DL  DD+EG+  LY
Sbjct: 291 DLESVAVHEIGHLLGLGHSSVEGSIMYPTIRTGRRKVDLTTDDVEGVQYLY 328

[Close]
BLAST of Cucsa.158780 vs. Swiss-Prot
Match: 1MMP_ARATH (Metalloendoproteinase 1-MMP OS=Arabidopsis thaliana GN=1MMP PE=1 SV=1)

HSP 1 Score: 152.5 bits (384), Expect = 7.0e-36
Identity = 99/269 (36.80%), Postives = 142/269 (52.79%), Query Frame = 1 

Query: 29  GHNIEGIHTIKMHLQRYGYLSKNYNIIDTNGAYNNAFDDHLESAIKKYQMFFKLPKSGVL 88
           G ++ G+  +K +L R+GY++      D +  +++ FD  LESAI  YQ    LP +G L
Sbjct: 64  GSHVSGVSELKRYLHRFGYVN------DGSEIFSDVFDGPLESAISLYQENLGLPITGRL 123

Query: 89  DMETLHQMSQARCSVPDIFENNENETSVTTSN--LHIGSKYTFFPGRVKWP-DSLNYRL- 148
           D  T+  MS  RC V D        T +T +N  LH  + YT+F G+ KW  D+L Y + 
Sbjct: 124 DTSTVTLMSLPRCGVSD--------THMTINNDFLHTTAHYTYFNGKPKWNRDTLTYAIS 183

Query: 149 -TYALVNNFPEEFKESVRTAFEIWYGRSRFNFTEVSENEGGNIRISFERGVHGDYHPFTK 208
            T+ L     E+ K   R AF  W      +F EV +    +++I F  G HGD  PF  
Sbjct: 184 KTHKLDYLTSEDVKTVFRRAFSQWSSVIPVSFEEVDDFTTADLKIGFYAGDHGDGLPFDG 243

Query: 209 NSKTLAHTFAPIDGRFHFNADKPFSVDVTYN-----AYHLRTVALHELGHAFGLAHSPSE 268
              TLAH FAP +GR H +A + + VD         A  L +VA HE+GH  GL HS  E
Sbjct: 244 VLGTLAHAFAPENGRLHLDAAETWIVDDDLKGSSEVAVDLESVATHEIGHLLGLGHSSQE 303

Query: 269 DAIMFPTI-PTNLEKDLDMDDIEGLWELY 287
            A+M+P++ P   + DL +DD+ G+ +LY
Sbjct: 304 SAVMYPSLRPRTKKVDLTVDDVAGVLKLY 318

[Close]
BLAST of Cucsa.158780 vs. TrEMBL
Match: A0A0A0KS15_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_5G632090 PE=3 SV=1)

HSP 1 Score: 612.8 bits (1579), Expect = 2.1e-172
Identity = 292/292 (100.00%), Postives = 292/292 (100.00%), Query Frame = 1 

Query: 1   FPFIWARDLHDIKKSQLLFPQHLLGSSKGHNIEGIHTIKMHLQRYGYLSKNYNIIDTNGA 60
           FPFIWARDLHDIKKSQLLFPQHLLGSSKGHNIEGIHTIKMHLQRYGYLSKNYNIIDTNGA
Sbjct: 22  FPFIWARDLHDIKKSQLLFPQHLLGSSKGHNIEGIHTIKMHLQRYGYLSKNYNIIDTNGA 81

Query: 61  YNNAFDDHLESAIKKYQMFFKLPKSGVLDMETLHQMSQARCSVPDIFENNENETSVTTSN 120
           YNNAFDDHLESAIKKYQMFFKLPKSGVLDMETLHQMSQARCSVPDIFENNENETSVTTSN
Sbjct: 82  YNNAFDDHLESAIKKYQMFFKLPKSGVLDMETLHQMSQARCSVPDIFENNENETSVTTSN 141

Query: 121 LHIGSKYTFFPGRVKWPDSLNYRLTYALVNNFPEEFKESVRTAFEIWYGRSRFNFTEVSE 180
           LHIGSKYTFFPGRVKWPDSLNYRLTYALVNNFPEEFKESVRTAFEIWYGRSRFNFTEVSE
Sbjct: 142 LHIGSKYTFFPGRVKWPDSLNYRLTYALVNNFPEEFKESVRTAFEIWYGRSRFNFTEVSE 201

Query: 181 NEGGNIRISFERGVHGDYHPFTKNSKTLAHTFAPIDGRFHFNADKPFSVDVTYNAYHLRT 240
           NEGGNIRISFERGVHGDYHPFTKNSKTLAHTFAPIDGRFHFNADKPFSVDVTYNAYHLRT
Sbjct: 202 NEGGNIRISFERGVHGDYHPFTKNSKTLAHTFAPIDGRFHFNADKPFSVDVTYNAYHLRT 261

Query: 241 VALHELGHAFGLAHSPSEDAIMFPTIPTNLEKDLDMDDIEGLWELYDGFGVA 293
           VALHELGHAFGLAHSPSEDAIMFPTIPTNLEKDLDMDDIEGLWELYDGFGVA
Sbjct: 262 VALHELGHAFGLAHSPSEDAIMFPTIPTNLEKDLDMDDIEGLWELYDGFGVA 313

[Close]
BLAST of Cucsa.158780 vs. TrEMBL
Match: A0A0A0KX75_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_5G632100 PE=3 SV=1)

HSP 1 Score: 383.3 bits (983), Expect = 2.7e-103
Identity = 183/289 (63.32%), Postives = 222/289 (76.82%), Query Frame = 1 

Query: 4   IWARDLHDIK-KSQLLFPQHLLGSSKGHNIEGIHTIKMHLQRYGYLSKNYNIIDTNGAYN 63
           I +RDL+ I   SQ LFP++L+GS KGHN+EG HTIK +LQRYGYLSK+YNIIDTNG YN
Sbjct: 24  ISSRDLNHIHGPSQFLFPKYLIGSRKGHNLEGTHTIKTYLQRYGYLSKSYNIIDTNGVYN 83

Query: 64  NAFDDHLESAIKKYQMFFKLPKSGVLDMETLHQMSQARCSVPDIFENNENETSVTTSNLH 123
           NA+D+ LES+IKKYQ FF+L +SG+LD ETL QMSQ+RCSVPD FE+++NETS+TTSNLH
Sbjct: 84  NAYDEQLESSIKKYQKFFQLNQSGILDTETLRQMSQSRCSVPDFFESDDNETSMTTSNLH 143

Query: 124 IGSKYTFFPGRVKWPDSLNYRLTYALVNNFPEEFKESVRTAFEIWYGRSRFNFTEVSENE 183
           +GS++ FFPGR KWPDS NY LT++ +NNFP  FK  V  AF  WY RSRF FTEV E E
Sbjct: 144 MGSRFKFFPGRPKWPDSKNYSLTFSFINNFPGIFKGEVGDAFLAWYERSRFFFTEVPEGE 203

Query: 184 GGNIRISFERGVHGDYHPFTKNSKTLAHTFAPIDGRFHFNADKPFSVDVTYNAYHLRTVA 243
             +I+ISFE G HGD HPF      LAH F P DGR HFN D+PFS +V    YH+R+VA
Sbjct: 204 ESDIKISFEVGDHGDGHPFRVG--VLAHAFTPTDGRLHFNGDEPFSSEVAEGKYHVRSVA 263

Query: 244 LHELGHAFGLAHSPSEDAIMFPTIPTNLEKDLDMDDIEGLWELYDGFGV 292
           LHELGH+ GLAH+   DAIM+PT+P N  K ++ DD+ GLW LYD F +
Sbjct: 264 LHELGHSLGLAHTEILDAIMYPTLPPNFAKSINSDDVNGLWALYDNFHI 310

[Close]
BLAST of Cucsa.158780 vs. TrEMBL
Match: A0A0A0KNY8_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_5G522910 PE=3 SV=1)

HSP 1 Score: 233.8 bits (595), Expect = 2.7e-58
Identity = 129/278 (46.40%), Postives = 165/278 (59.35%), Query Frame = 1 

Query: 13  KKSQLLFPQHLLGSSKGHNIEGIHTIKMHLQRYGYLSKNYNI----IDTNGAYNNAFDDH 72
           K + +L PQ L G  KG N+EGIH+IK +LQRYGYLS N +I    I+ N   +N FDD 
Sbjct: 23  KHNPILSPQRLYGCRKGDNVEGIHSIKKYLQRYGYLSHNTSIDSHIIELN---SNKFDDS 82

Query: 73  LESAIKKYQMFFKLPKSGVLDMETLHQMSQARCSVPDIFENNENETSVTTSNLHIGSKYT 132
           LESAIK YQ +  L  SG+LD ETL QM + RC V D+F+ N N+      +L + S YT
Sbjct: 83  LESAIKLYQKWSHLNVSGILDQETLDQMFKPRCGVRDVFKFNSNKN--LEDDLEMSSHYT 142

Query: 133 FFPGRVKWPDSLNYRLTYALVNNFPEEFKESVRTAFEIWYGRSRFNFTEVSENEGGNIRI 192
            FPG +KWPD   + LTY   NNFP +F  SV  A   W  +S F F+E S+ +  +I I
Sbjct: 143 LFPGNLKWPDYKRH-LTYVFTNNFPVDFVPSVTEAMARWAAQSLFTFSEASDAQSADINI 202

Query: 193 SFERGVHGDYHPFTKNSKTLAHTFAPIDGRFHFNADKPFSVDVTYNAYHLRTVALHELGH 252
           SF+   H D  PF      + H FAP DGR H + D  +S  +     ++  VALHELGH
Sbjct: 203 SFQIKDHADGLPFDGPGGVVGHAFAPTDGRLHLDGDDTWSAGMEVQKVNVMNVALHELGH 262

Query: 253 AFGLAHSPSEDAIMFPTIPTNLEKDLDMDDIEGLWELY 287
             GLAHS    A+M+P I +N  K+L+ DDI GL  LY
Sbjct: 263 VLGLAHSTLPQAVMWPYIDSNALKNLNDDDIAGLHALY 294

[Close]
BLAST of Cucsa.158780 vs. TrEMBL
Match: A0A0A0KS35_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_5G632070 PE=3 SV=1)

HSP 1 Score: 233.4 bits (594), Expect = 3.5e-58
Identity = 133/289 (46.02%), Postives = 176/289 (60.90%), Query Frame = 1 

Query: 6   ARDLHDIKKSQLLFPQHLLGSSKGHNI-EGIHTIKMHLQRYGYLSKNYNIIDTNGAYNNA 65
           AR LH+ K S+LLFP+HL+GSSK  +I EGIH +K +LQRYGY S      + N    +A
Sbjct: 23  ARPLHNHKSSRLLFPEHLIGSSKNDSIVEGIHKVKAYLQRYGYFSNE----NENNLSTDA 82

Query: 66  FDDHLESAIKKYQMFFKLPKSGVLDMETLHQMSQARCSVPDIFEN---NENETSVTTSNL 125
           FDD LESAIK YQ F  L  SGVLD ETL QMS+ RC V D F++    + E + TT  +
Sbjct: 83  FDDDLESAIKSYQKFSNLKVSGVLDRETLQQMSRPRCGVADNFQSVAQQDGENNNTTVQI 142

Query: 126 HIGSKYTFFPGRVKWPDSLNYRLTYALVNNFPEEFKESVRTAFEIWYGRSRFNFTEVSEN 185
             GS + FFPG+ KWP    + LTY  V+N+P +   +V  AF+ W   S+F F+     
Sbjct: 143 G-GSHFMFFPGKGKWPYR-KWHLTYGFVHNYPMKHAAAVVRAFDKWAANSKFTFSLAWRI 202

Query: 186 EGGNIRISFERGVHGDYHPFTKNSKTLAHTFAPIDGRFHFNADKPFSV--DVTYNAYHLR 245
           +  +I +SFERG HGD  PF      L H F PIDGR HF+AD+ ++    +T   +   
Sbjct: 203 QTADILLSFERGDHGDGKPFDGEGGILGHAFGPIDGRVHFDADEQWAEIGSLTNENFDFE 262

Query: 246 TVALHELGHAFGLAHSPSEDAIMFPTIPTNLEK-DLDMDDIEGLWELYD 288
           +VALHE+GHA GL HS    A+M+  + T + K +L +DDIEG+  L D
Sbjct: 263 SVALHEIGHALGLGHSIFPSAVMWANMETGVNKTELTIDDIEGVHALND 305

[Close]
BLAST of Cucsa.158780 vs. TrEMBL
Match: A0A0A0KVD8_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_5G633150 PE=3 SV=1)

HSP 1 Score: 227.3 bits (578), Expect = 2.5e-56
Identity = 125/276 (45.29%), Postives = 162/276 (58.70%), Query Frame = 1 

Query: 15  SQLLFPQHLLGSSKGHNIEGIHTIKMHLQRYGYLSKNY----NIIDTNGAYNNAFDDHLE 74
           +Q+L P+ L G  KG N+EGIH IK +LQRYGYLS N     NII+ N   +N FDD LE
Sbjct: 24  NQILSPRRLNGCRKGDNVEGIHNIKKYLQRYGYLSHNTSTDSNIIELN---SNKFDDSLE 83

Query: 75  SAIKKYQMFFKLPKSGVLDMETLHQMSQARCSVPDIFENNENETSVTTSNLHIGSKYTFF 134
           SAI+ YQ +  L  SG+LD ETL QM Q RC + D+F+ N ++      +L + S Y  F
Sbjct: 84  SAIRLYQKWSHLNVSGILDQETLDQMFQQRCGIRDVFKFNSSKN--LEDDLEMSSHYVLF 143

Query: 135 PGRVKWPDSLNYRLTYALVNNFPEEFKESVRTAFEIWYGRSRFNFTEVSENEGGNIRISF 194
           P   KWPD   + LTY   NNFP +F  SV  A   W  +S F F+E S+ +  +I ISF
Sbjct: 144 PNNKKWPDYKRH-LTYMFTNNFPVDFVPSVTEAMARWAAQSLFTFSEASDAQSADINISF 203

Query: 195 ERGVHGDYHPFTKNSKTLAHTFAPIDGRFHFNADKPFSVDVTYNAYHLRTVALHELGHAF 254
           +   H D   F      + H FAP DGR H + D  +S  +  N +++  VALHELGH  
Sbjct: 204 QVKDHADGSAFDGPGGVVGHAFAPTDGRLHLDGDDSWSAGLEENKFNVMNVALHELGHVL 263

Query: 255 GLAHSPSEDAIMFPTIPTNLEKDLDMDDIEGLWELY 287
           GLAHS    A+M+P I +N  K+L+ DDI GL  LY
Sbjct: 264 GLAHSTLPQAVMWPYIDSNALKNLNDDDIAGLHALY 293

[Close]
BLAST of Cucsa.158780 vs. TAIR10
Match: AT1G70170.1 (AT1G70170.1 matrix metalloproteinase)

HSP 1 Score: 165.2 bits (417), Expect = 5.9e-41
Identity = 100/281 (35.59%), Postives = 145/281 (51.60%), Query Frame = 1 

Query: 25  GSSKGHNIEGIHTIKMHLQRYGYLSKNYNIIDTNGAYNNAFDDHLESAIKKYQMFFKLPK 84
           G   G N++G++ IK + QR+GY+ + ++     G + + FDD L++A++ YQ  F L  
Sbjct: 49  GCHHGQNVDGLYRIKKYFQRFGYIPETFS-----GNFTDDFDDILKAAVELYQTNFNLNV 108

Query: 85  SGVLDMETLHQMSQARCSVPDIFENNEN---------ETSVTTSNLHIGSKYTFFPGRVK 144
           +G LD  T+  +   RC  PD+               E + + ++LH   +YT FPG  +
Sbjct: 109 TGELDALTIQHIVIPRCGNPDVVNGTSLMHGGRRKTFEVNFSRTHLHAVKRYTLFPGEPR 168

Query: 145 WPDSLNYRLTYAL--VNNFPEEFKESVRTAFEIWYGRSRFNFTEVSENEGGNIRISFERG 204
           WP +    LTYA    N   EE K     AF  W   +  NFT        +I I F  G
Sbjct: 169 WPRNRR-DLTYAFDPKNPLTEEVKSVFSRAFGRWSDVTALNFTLSESFSTSDITIGFYTG 228

Query: 205 VHGDYHPFTKNSKTLAHTFAPIDGRFHFNADKPFSVDVTYNAY-------HLRTVALHEL 264
            HGD  PF     TLAH F+P  G+FH +AD+ + V    +++        L +VA+HE+
Sbjct: 229 DHGDGEPFDGVLGTLAHAFSPPSGKFHLDADENWVVSGDLDSFLSVTAAVDLESVAVHEI 288

Query: 265 GHAFGLAHSPSEDAIMFPTIPTNLEK-DLDMDDIEGLWELY 287
           GH  GL HS  E++IM+PTI T   K DL  DD+EG+  LY
Sbjct: 289 GHLLGLGHSSVEESIMYPTITTGKRKVDLTNDDVEGIQYLY 323

[Close]
BLAST of Cucsa.158780 vs. TAIR10
Match: AT1G59970.1 (AT1G59970.1 Matrixin family protein)

HSP 1 Score: 156.8 bits (395), Expect = 2.1e-38
Identity = 102/278 (36.69%), Postives = 132/278 (47.48%), Query Frame = 1 

Query: 23  LLGSSKGHNIEGIHTIKMHLQRYGYLSKNYNIIDTNGAYNNAFDDHLESAIKKYQMFFKL 82
           L G   G NI G+  +K + +R+GY++       T G   + FDD L+SAI  YQ  F L
Sbjct: 48  LAGCHIGENINGLSKLKQYFRRFGYIT-------TTGNCTDDFDDVLQSAINTYQKNFNL 107

Query: 83  PKSGVLDMETLHQMSQARCSVPDIFENNENETSVTTSNLHIGSKYTFFPGRVKWPDSLNY 142
             +G LD  TL Q+ + RC  PD+ +            L    KY+FFPG+ +WP     
Sbjct: 108 KVTGKLDSSTLRQIVKPRCGNPDLIDGVSEMNG--GKILRTTEKYSFFPGKPRWP-KRKR 167

Query: 143 RLTYALV--NNFPEEFKESVRTAFEIWYGRSRFNFTEVSENEGGNIRISFERGVHGDYHP 202
            LTYA    NN  +E K     AF  W   +  NFT        +I I F  G HGD  P
Sbjct: 168 DLTYAFAPQNNLTDEVKRVFSRAFTRWAEVTPLNFTRSESILRADIVIGFFSGEHGDGEP 227

Query: 203 FTKNSKTLAHTFAPIDGRFHFNADKPFSVD---------VTYNAYHLRTVALHELGHAFG 262
           F     TLAH  +P  G  H + D+ + +                 L +VA+HE+GH  G
Sbjct: 228 FDGAMGTLAHASSPPTGMLHLDGDEDWLISNGEISRRILPVTTVVDLESVAVHEIGHLLG 287

Query: 263 LAHSPSEDAIMFPTIPTNLEK-DLDMDDIEGLWELYDG 289
           L HS  EDAIMFP I     K +L  DDIEG+  LY G
Sbjct: 288 LGHSSVEDAIMFPAISGGDRKVELAKDDIEGIQHLYGG 315

[Close]
BLAST of Cucsa.158780 vs. TAIR10
Match: AT1G24140.1 (AT1G24140.1 Matrixin family protein)

HSP 1 Score: 153.3 bits (386), Expect = 2.3e-37
Identity = 100/291 (34.36%), Postives = 140/291 (48.11%), Query Frame = 1 

Query: 25  GSSKGHNIEGIHTIKMHLQRYGYLSKNYNIIDTNGAYNNAFDDHLESAIKKYQMFFKLPK 84
           G   G   +G++ +K + Q +GY+++     + +G + + FDD L++A++ YQ  F+L  
Sbjct: 51  GCHAGKKYDGLYMLKQYFQHFGYITET----NLSGNFTDDFDDILKNAVEMYQRNFQLNV 110

Query: 85  SGVLDMETLHQMSQARCSVPDIFENNENETSVTTSNLHIGSK------------------ 144
           +GVLD  TL  +   RC  PD+           TS +H G K                  
Sbjct: 111 TGVLDELTLKHVVIPRCGNPDVVNG--------TSTMHSGRKTFEVSFAGRGQRFHAVKH 170

Query: 145 YTFFPGRVKWPDSLNYRLTYALV--NNFPEEFKESVRTAFEIWYGRSRFNFTEVSENEGG 204
           Y+FFPG  +WP +    LTYA    N   EE K     AF  W   +   FT V      
Sbjct: 171 YSFFPGEPRWPRNRR-DLTYAFDPRNALTEEVKSVFSRAFTRWEEVTPLTFTRVERFSTS 230

Query: 205 NIRISFERGVHGDYHPFTKNSKTLAHTFAPIDGRFHFNADKPFSVD--------VTYNAY 264
           +I I F  G HGD  PF    +TLAH F+P  G FH + ++ + V             A 
Sbjct: 231 DISIGFYSGEHGDGEPFDGPMRTLAHAFSPPTGHFHLDGEENWIVSGEGGDGFISVSEAV 290

Query: 265 HLRTVALHELGHAFGLAHSPSEDAIMFPTIPTNLEK-DLDMDDIEGLWELY 287
            L +VA+HE+GH  GL HS  E +IM+PTI T   K DL  DD+EG+  LY
Sbjct: 291 DLESVAVHEIGHLLGLGHSSVEGSIMYPTIRTGRRKVDLTTDDVEGVQYLY 328

[Close]
BLAST of Cucsa.158780 vs. TAIR10
Match: AT4G16640.1 (AT4G16640.1 Matrixin family protein)

HSP 1 Score: 152.5 bits (384), Expect = 4.0e-37
Identity = 99/269 (36.80%), Postives = 142/269 (52.79%), Query Frame = 1 

Query: 29  GHNIEGIHTIKMHLQRYGYLSKNYNIIDTNGAYNNAFDDHLESAIKKYQMFFKLPKSGVL 88
           G ++ G+  +K +L R+GY++      D +  +++ FD  LESAI  YQ    LP +G L
Sbjct: 64  GSHVSGVSELKRYLHRFGYVN------DGSEIFSDVFDGPLESAISLYQENLGLPITGRL 123

Query: 89  DMETLHQMSQARCSVPDIFENNENETSVTTSN--LHIGSKYTFFPGRVKWP-DSLNYRL- 148
           D  T+  MS  RC V D        T +T +N  LH  + YT+F G+ KW  D+L Y + 
Sbjct: 124 DTSTVTLMSLPRCGVSD--------THMTINNDFLHTTAHYTYFNGKPKWNRDTLTYAIS 183

Query: 149 -TYALVNNFPEEFKESVRTAFEIWYGRSRFNFTEVSENEGGNIRISFERGVHGDYHPFTK 208
            T+ L     E+ K   R AF  W      +F EV +    +++I F  G HGD  PF  
Sbjct: 184 KTHKLDYLTSEDVKTVFRRAFSQWSSVIPVSFEEVDDFTTADLKIGFYAGDHGDGLPFDG 243

Query: 209 NSKTLAHTFAPIDGRFHFNADKPFSVDVTYN-----AYHLRTVALHELGHAFGLAHSPSE 268
              TLAH FAP +GR H +A + + VD         A  L +VA HE+GH  GL HS  E
Sbjct: 244 VLGTLAHAFAPENGRLHLDAAETWIVDDDLKGSSEVAVDLESVATHEIGHLLGLGHSSQE 303

Query: 269 DAIMFPTI-PTNLEKDLDMDDIEGLWELY 287
            A+M+P++ P   + DL +DD+ G+ +LY
Sbjct: 304 SAVMYPSLRPRTKKVDLTVDDVAGVLKLY 318

[Close]
BLAST of Cucsa.158780 vs. TAIR10
Match: AT2G45040.1 (AT2G45040.1 Matrixin family protein)

HSP 1 Score: 150.6 bits (379), Expect = 1.5e-36
Identity = 94/263 (35.74%), Postives = 131/263 (49.81%), Query Frame = 1 

Query: 35  IHTIKMHLQRYGYLSKNYNIIDTNGAYNNAFDDHLESAIKKYQMFFKLPKSGVLDMETLH 94
           I  IK HLQ+YGYL +N    D +           E A+ +YQ    LP +G  D +TL 
Sbjct: 50  IPEIKRHLQQYGYLPQNKESDDVS----------FEQALVRYQKNLGLPITGKPDSDTLS 109

Query: 95  QMSQARCSVPDIFENNENETSVTTSNLHIGSKYTFFPGRVKWPDSLNYRLTYALVNN--- 154
           Q+   RC  PD  E         T+  H G KY +FPGR +W   +  +LTYA       
Sbjct: 110 QILLPRCGFPDDVEPK-------TAPFHTGKKYVYFPGRPRWTRDVPLKLTYAFSQENLT 169

Query: 155 ---FPEEFKESVRTAFEIWYGRSRFNFTEVSENEGGNIRISFERGVHGDYHPFTKNSKTL 214
               P + +   R AF  W      +F E  +    +I+I F  G HGD  PF      L
Sbjct: 170 PYLAPTDIRRVFRRAFGKWASVIPVSFIETEDYVIADIKIGFFNGDHGDGEPFDGVLGVL 229

Query: 215 AHTFAPIDGRFHFNADKPFSVDVTYN----AYHLRTVALHELGHAFGLAHSPSEDAIMFP 274
           AHTF+P +GR H +  + ++VD        A  L +VA+HE+GH  GL HS  +DA M+P
Sbjct: 230 AHTFSPENGRLHLDKAETWAVDFDEEKSSVAVDLESVAVHEIGHVLGLGHSSVKDAAMYP 289

Query: 275 TI-PTNLEKDLDMDDIEGLWELY 287
           T+ P + + +L+MDD+ G+  LY
Sbjct: 290 TLKPRSKKVNLNMDDVVGVQSLY 295

[Close]
BLAST of Cucsa.158780 vs. NCBI nr
Match: gi|449449423|ref|XP_004142464.1| (PREDICTED: metalloendoproteinase 1-like [Cucumis sativus])

HSP 1 Score: 612.8 bits (1579), Expect = 3.0e-172
Identity = 292/292 (100.00%), Postives = 292/292 (100.00%), Query Frame = 1 

Query: 1   FPFIWARDLHDIKKSQLLFPQHLLGSSKGHNIEGIHTIKMHLQRYGYLSKNYNIIDTNGA 60
           FPFIWARDLHDIKKSQLLFPQHLLGSSKGHNIEGIHTIKMHLQRYGYLSKNYNIIDTNGA
Sbjct: 22  FPFIWARDLHDIKKSQLLFPQHLLGSSKGHNIEGIHTIKMHLQRYGYLSKNYNIIDTNGA 81

Query: 61  YNNAFDDHLESAIKKYQMFFKLPKSGVLDMETLHQMSQARCSVPDIFENNENETSVTTSN 120
           YNNAFDDHLESAIKKYQMFFKLPKSGVLDMETLHQMSQARCSVPDIFENNENETSVTTSN
Sbjct: 82  YNNAFDDHLESAIKKYQMFFKLPKSGVLDMETLHQMSQARCSVPDIFENNENETSVTTSN 141

Query: 121 LHIGSKYTFFPGRVKWPDSLNYRLTYALVNNFPEEFKESVRTAFEIWYGRSRFNFTEVSE 180
           LHIGSKYTFFPGRVKWPDSLNYRLTYALVNNFPEEFKESVRTAFEIWYGRSRFNFTEVSE
Sbjct: 142 LHIGSKYTFFPGRVKWPDSLNYRLTYALVNNFPEEFKESVRTAFEIWYGRSRFNFTEVSE 201

Query: 181 NEGGNIRISFERGVHGDYHPFTKNSKTLAHTFAPIDGRFHFNADKPFSVDVTYNAYHLRT 240
           NEGGNIRISFERGVHGDYHPFTKNSKTLAHTFAPIDGRFHFNADKPFSVDVTYNAYHLRT
Sbjct: 202 NEGGNIRISFERGVHGDYHPFTKNSKTLAHTFAPIDGRFHFNADKPFSVDVTYNAYHLRT 261

Query: 241 VALHELGHAFGLAHSPSEDAIMFPTIPTNLEKDLDMDDIEGLWELYDGFGVA 293
           VALHELGHAFGLAHSPSEDAIMFPTIPTNLEKDLDMDDIEGLWELYDGFGVA
Sbjct: 262 VALHELGHAFGLAHSPSEDAIMFPTIPTNLEKDLDMDDIEGLWELYDGFGVA 313

[Close]
BLAST of Cucsa.158780 vs. NCBI nr
Match: gi|700197234|gb|KGN52411.1| (hypothetical protein Csa_5G632100 [Cucumis sativus])

HSP 1 Score: 383.3 bits (983), Expect = 3.9e-103
Identity = 183/289 (63.32%), Postives = 222/289 (76.82%), Query Frame = 1 

Query: 4   IWARDLHDIK-KSQLLFPQHLLGSSKGHNIEGIHTIKMHLQRYGYLSKNYNIIDTNGAYN 63
           I +RDL+ I   SQ LFP++L+GS KGHN+EG HTIK +LQRYGYLSK+YNIIDTNG YN
Sbjct: 24  ISSRDLNHIHGPSQFLFPKYLIGSRKGHNLEGTHTIKTYLQRYGYLSKSYNIIDTNGVYN 83

Query: 64  NAFDDHLESAIKKYQMFFKLPKSGVLDMETLHQMSQARCSVPDIFENNENETSVTTSNLH 123
           NA+D+ LES+IKKYQ FF+L +SG+LD ETL QMSQ+RCSVPD FE+++NETS+TTSNLH
Sbjct: 84  NAYDEQLESSIKKYQKFFQLNQSGILDTETLRQMSQSRCSVPDFFESDDNETSMTTSNLH 143

Query: 124 IGSKYTFFPGRVKWPDSLNYRLTYALVNNFPEEFKESVRTAFEIWYGRSRFNFTEVSENE 183
           +GS++ FFPGR KWPDS NY LT++ +NNFP  FK  V  AF  WY RSRF FTEV E E
Sbjct: 144 MGSRFKFFPGRPKWPDSKNYSLTFSFINNFPGIFKGEVGDAFLAWYERSRFFFTEVPEGE 203

Query: 184 GGNIRISFERGVHGDYHPFTKNSKTLAHTFAPIDGRFHFNADKPFSVDVTYNAYHLRTVA 243
             +I+ISFE G HGD HPF      LAH F P DGR HFN D+PFS +V    YH+R+VA
Sbjct: 204 ESDIKISFEVGDHGDGHPFRVG--VLAHAFTPTDGRLHFNGDEPFSSEVAEGKYHVRSVA 263

Query: 244 LHELGHAFGLAHSPSEDAIMFPTIPTNLEKDLDMDDIEGLWELYDGFGV 292
           LHELGH+ GLAH+   DAIM+PT+P N  K ++ DD+ GLW LYD F +
Sbjct: 264 LHELGHSLGLAHTEILDAIMYPTLPPNFAKSINSDDVNGLWALYDNFHI 310

[Close]
BLAST of Cucsa.158780 vs. NCBI nr
Match: gi|659118021|ref|XP_008458908.1| (PREDICTED: metalloendoproteinase 1-like [Cucumis melo])

HSP 1 Score: 365.5 bits (937), Expect = 8.4e-98
Identity = 179/284 (63.03%), Postives = 214/284 (75.35%), Query Frame = 1 

Query: 4   IWARDLHDIK-KSQLLFPQHLLGSSKGHNIEGIHTIKMHLQRYGYLSKNYNIIDTNGAYN 63
           I +RDL+ I   SQ LFP++LLGS KGHNIEG H IK +LQ YGYLSKNYNIIDTNG YN
Sbjct: 23  ISSRDLNHIHGPSQFLFPKYLLGSRKGHNIEGTHAIKTYLQHYGYLSKNYNIIDTNGVYN 82

Query: 64  NAFDDHLESAIKKYQMFFKLPKSGVLDMETLHQMSQARCSVPDIFENNENETSVTTSNLH 123
           NA+D+ LE ++KKYQ FFKL +SG+LD ETL QMSQ RCSVPDIFE+ +NETS+TTSNLH
Sbjct: 83  NAYDEQLECSVKKYQKFFKLNESGILDRETLSQMSQPRCSVPDIFESEDNETSITTSNLH 142

Query: 124 IGSKYTFFPGRVKWPDSLNYRLTYALVNNFPEEFKESVRTAFEIWYGRSRFNFTEVSENE 183
           I S+Y FFPGR KW DS N  LTY+L+ NFPE+F+ ++  AF +W+ RSRF FT VSE +
Sbjct: 143 IRSRYAFFPGRPKWSDSRN--LTYSLIKNFPEKFEAAIVDAFMLWFLRSRFCFTYVSEGQ 202

Query: 184 GGNIRISFERGVHGDYHPFTKNSKTLAHTFAPIDGRFHFNADKPFSVDVTYNAYHLRTVA 243
             +I ISFE G HGD HPF      LAH F+P DGR HFN DK FS D+    YH+RTVA
Sbjct: 203 KSDITISFEVGDHGDGHPF--KGGVLAHAFSPEDGRLHFNGDKSFSSDLEEGKYHIRTVA 262

Query: 244 LHELGHAFGLAHSPSEDAIMFPTIPTNLEKDLDMDDIEGLWELY 287
            HELGH+ GLAH+  E AIM+PT+P+N  K L  DD++GLW  Y
Sbjct: 263 AHELGHSLGLAHTNIEAAIMYPTLPSNFSKILSQDDVDGLWNFY 302

[Close]
BLAST of Cucsa.158780 vs. NCBI nr
Match: gi|778708743|ref|XP_011656274.1| (PREDICTED: metalloendoproteinase 1-like [Cucumis sativus])

HSP 1 Score: 255.0 bits (650), Expect = 1.6e-64
Identity = 119/196 (60.71%), Postives = 144/196 (73.47%), Query Frame = 1 

Query: 96  MSQARCSVPDIFENNENETSVTTSNLHIGSKYTFFPGRVKWPDSLNYRLTYALVNNFPEE 155
           MSQ+RCSVPD FE+++NETS+TTSNLH+GS++ FFPGR KWPDS NY LT++ +NNFP  
Sbjct: 1   MSQSRCSVPDFFESDDNETSMTTSNLHMGSRFKFFPGRPKWPDSKNYSLTFSFINNFPGI 60

Query: 156 FKESVRTAFEIWYGRSRFNFTEVSENEGGNIRISFERGVHGDYHPFTKNSKTLAHTFAPI 215
           FK  V  AF  WY RSRF FTEV E E  +I+ISFE G HGD HPF      LAH F P 
Sbjct: 61  FKGEVGDAFLAWYERSRFFFTEVPEGEESDIKISFEVGDHGDGHPFRVG--VLAHAFTPT 120

Query: 216 DGRFHFNADKPFSVDVTYNAYHLRTVALHELGHAFGLAHSPSEDAIMFPTIPTNLEKDLD 275
           DGR HFN D+PFS +V    YH+R+VALHELGH+ GLAH+   DAIM+PT+P N  K ++
Sbjct: 121 DGRLHFNGDEPFSSEVAEGKYHVRSVALHELGHSLGLAHTEILDAIMYPTLPPNFAKSIN 180

Query: 276 MDDIEGLWELYDGFGV 292
            DD+ GLW LYD F +
Sbjct: 181 SDDVNGLWALYDNFHI 194

[Close]
BLAST of Cucsa.158780 vs. NCBI nr
Match: gi|778708586|ref|XP_004142843.2| (PREDICTED: metalloendoproteinase 1-like [Cucumis sativus])

HSP 1 Score: 233.8 bits (595), Expect = 3.8e-58
Identity = 129/278 (46.40%), Postives = 165/278 (59.35%), Query Frame = 1 

Query: 13  KKSQLLFPQHLLGSSKGHNIEGIHTIKMHLQRYGYLSKNYNI----IDTNGAYNNAFDDH 72
           K + +L PQ L G  KG N+EGIH+IK +LQRYGYLS N +I    I+ N   +N FDD 
Sbjct: 23  KHNPILSPQRLYGCRKGDNVEGIHSIKKYLQRYGYLSHNTSIDSHIIELN---SNKFDDS 82

Query: 73  LESAIKKYQMFFKLPKSGVLDMETLHQMSQARCSVPDIFENNENETSVTTSNLHIGSKYT 132
           LESAIK YQ +  L  SG+LD ETL QM + RC V D+F+ N N+      +L + S YT
Sbjct: 83  LESAIKLYQKWSHLNVSGILDQETLDQMFKPRCGVRDVFKFNSNKN--LEDDLEMSSHYT 142

Query: 133 FFPGRVKWPDSLNYRLTYALVNNFPEEFKESVRTAFEIWYGRSRFNFTEVSENEGGNIRI 192
            FPG +KWPD   + LTY   NNFP +F  SV  A   W  +S F F+E S+ +  +I I
Sbjct: 143 LFPGNLKWPDYKRH-LTYVFTNNFPVDFVPSVTEAMARWAAQSLFTFSEASDAQSADINI 202

Query: 193 SFERGVHGDYHPFTKNSKTLAHTFAPIDGRFHFNADKPFSVDVTYNAYHLRTVALHELGH 252
           SF+   H D  PF      + H FAP DGR H + D  +S  +     ++  VALHELGH
Sbjct: 203 SFQIKDHADGLPFDGPGGVVGHAFAPTDGRLHLDGDDTWSAGMEVQKVNVMNVALHELGH 262

Query: 253 AFGLAHSPSEDAIMFPTIPTNLEKDLDMDDIEGLWELY 287
             GLAHS    A+M+P I +N  K+L+ DDI GL  LY
Sbjct: 263 VLGLAHSTLPQAVMWPYIDSNALKNLNDDDIAGLHALY 294

The following BLAST results are available for this feature:
BLAST of Cucsa.158780 vs. Swiss-Prot
Match NameE-valueIdentityDescription
2MMP_ARATH1.0e-3935.59Metalloendoproteinase 2-MMP OS=Arabidopsis thaliana GN=2MMP PE=1 SV=1[more]
5MMP_ARATH3.7e-3736.69Metalloendoproteinase 5-MMP OS=Arabidopsis thaliana GN=5MMP PE=1 SV=1[more]
MEP1_SOYBN2.4e-3637.83Metalloendoproteinase 1 OS=Glycine max PE=1 SV=2[more]
3MMP_ARATH4.1e-3634.36Metalloendoproteinase 3-MMP OS=Arabidopsis thaliana GN=3MMP PE=1 SV=1[more]
1MMP_ARATH7.0e-3636.80Metalloendoproteinase 1-MMP OS=Arabidopsis thaliana GN=1MMP PE=1 SV=1[more]
BLAST of Cucsa.158780 vs. TrEMBL
Match NameE-valueIdentityDescription
A0A0A0KS15_CUCSA2.1e-172100.00Uncharacterized protein OS=Cucumis sativus GN=Csa_5G632090 PE=3 SV=1[more]
A0A0A0KX75_CUCSA2.7e-10363.32Uncharacterized protein OS=Cucumis sativus GN=Csa_5G632100 PE=3 SV=1[more]
A0A0A0KNY8_CUCSA2.7e-5846.40Uncharacterized protein OS=Cucumis sativus GN=Csa_5G522910 PE=3 SV=1[more]
A0A0A0KS35_CUCSA3.5e-5846.02Uncharacterized protein OS=Cucumis sativus GN=Csa_5G632070 PE=3 SV=1[more]
A0A0A0KVD8_CUCSA2.5e-5645.29Uncharacterized protein OS=Cucumis sativus GN=Csa_5G633150 PE=3 SV=1[more]
BLAST of Cucsa.158780 vs. TAIR10
Match NameE-valueIdentityDescription
AT1G70170.15.9e-4135.59 matrix metalloproteinase[more]
AT1G59970.12.1e-3836.69 Matrixin family protein[more]
AT1G24140.12.3e-3734.36 Matrixin family protein[more]
AT4G16640.14.0e-3736.80 Matrixin family protein[more]
AT2G45040.11.5e-3635.74 Matrixin family protein[more]
BLAST of Cucsa.158780 vs. NCBI nr
Match NameE-valueIdentityDescription
gi|449449423|ref|XP_004142464.1|3.0e-172100.00PREDICTED: metalloendoproteinase 1-like [Cucumis sativus][more]
gi|700197234|gb|KGN52411.1|3.9e-10363.32hypothetical protein Csa_5G632100 [Cucumis sativus][more]
gi|659118021|ref|XP_008458908.1|8.4e-9863.03PREDICTED: metalloendoproteinase 1-like [Cucumis melo][more]
gi|778708743|ref|XP_011656274.1|1.6e-6460.71PREDICTED: metalloendoproteinase 1-like [Cucumis sativus][more]
gi|778708586|ref|XP_004142843.2|3.8e-5846.40PREDICTED: metalloendoproteinase 1-like [Cucumis sativus][more]
Annotated Terms
The following terms have been associated with this gene:
Vocabulary: INTERPRO
TermDefinition
IPR001818Pept_M10_metallopeptidase
IPR002477Peptidoglycan-bd-like
IPR006026Peptidase_Metallo
IPR021190Pept_M10A
IPR024079MetalloPept_cat_dom_sf
Vocabulary: Molecular Function
TermDefinition
GO:0004222metalloendopeptidase activity
GO:0008270zinc ion binding
GO:0008237metallopeptidase activity
Vocabulary: Biological Process
TermDefinition
GO:0006508proteolysis
Vocabulary: Cellular Component
TermDefinition
GO:0031012extracellular matrix
GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006508 proteolysis
cellular_component GO:0031012 extracellular matrix
molecular_function GO:0004222 metalloendopeptidase activity
molecular_function GO:0008270 zinc ion binding
molecular_function GO:0008237 metallopeptidase activity
Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Cucsa.158780.1Cucsa.158780.1mRNA


InterPro
Analysis Name: InterPro Annotations of cucumber (Gy14)
Date Performed: 2017-01-17
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001818Peptidase M10, metallopeptidasePFAMPF00413Peptidase_M10coord: 143..286
score: 3.1
IPR002477Peptidoglycan binding-likeGENE3DG3DSA:1.10.101.10coord: 34..98
score: 1.5
IPR002477Peptidoglycan binding-likePFAMPF01471PG_binding_1coord: 33..96
score: 1.2
IPR002477Peptidoglycan binding-likeunknownSSF47090PGBD-likecoord: 28..106
score: 7.59
IPR006026Peptidase, metallopeptidaseSMARTSM00235col_5coord: 132..288
score: 1.4
IPR021190Peptidase M10APRINTSPR00138MATRIXINcoord: 96..109
score: 2.8E-26coord: 241..266
score: 2.8E-26coord: 274..287
score: 2.8E-26coord: 160..175
score: 2.8E-26coord: 184..212
score: 2.8
IPR024079Metallopeptidase, catalytic domainGENE3DG3DSA:3.40.390.10coord: 99..287
score: 2.3
NoneNo IPR availablePANTHERPTHR10201MATRIX METALLOPROTEINASEcoord: 21..286
score: 7.0
NoneNo IPR availablePANTHERPTHR10201:SF152SUBFAMILY NOT NAMEDcoord: 21..286
score: 7.0
NoneNo IPR availableunknownSSF55486Metalloproteases ("zincins"), catalytic domaincoord: 129..287
score: 6.36

Synteny
The following gene(s) are orthologous to this gene:
GeneOrthologueOrganismBlock
Cucsa.158780MELO3C021497.2Melon (DHL92) v3.6.1cgymedB265
Cucsa.158780CsaV3_5G037520Cucumber (Chinese Long) v3cgycucB244
The following gene(s) are paralogous to this gene:

None

The following block(s) are covering this gene:
GeneOrganismBlock
Cucsa.158780Cucurbita pepo (Zucchini)cgycpeB0424
Cucsa.158780Cucurbita pepo (Zucchini)cgycpeB0425
Cucsa.158780Bottle gourd (USVL1VR-Ls)cgylsiB251
Cucsa.158780Bottle gourd (USVL1VR-Ls)cgylsiB252
Cucsa.158780Melon (DHL92) v3.6.1cgymedB268
Cucsa.158780Silver-seed gourdcarcgyB0309
Cucsa.158780Silver-seed gourdcarcgyB0763
Cucsa.158780Cucumber (Chinese Long) v3cgycucB240
Cucsa.158780Watermelon (97103) v2cgywmbB270
Cucsa.158780Watermelon (97103) v2cgywmbB265
Cucsa.158780Wax gourdcgywgoB308
Cucsa.158780Wax gourdcgywgoB310
Cucsa.158780Cucumber (Gy14) v1cgycgyB036
Cucsa.158780Cucurbita maxima (Rimu)cgycmaB0439
Cucsa.158780Cucurbita maxima (Rimu)cgycmaB0441
Cucsa.158780Cucurbita moschata (Rifu)cgycmoB0437
Cucsa.158780Cucurbita moschata (Rifu)cgycmoB0439
Cucsa.158780Cucurbita moschata (Rifu)cgycmoB0442
Cucsa.158780Cucumber (Chinese Long) v2cgycuB226
Cucsa.158780Wild cucumber (PI 183967)cgycpiB232
Cucsa.158780Wild cucumber (PI 183967)cgycpiB236
Cucsa.158780Cucumber (Chinese Long) v2cgycuB230
Cucsa.158780Melon (DHL92) v3.5.1cgymeB265
Cucsa.158780Melon (DHL92) v3.5.1cgymeB268
Cucsa.158780Watermelon (Charleston Gray)cgywcgB264
Cucsa.158780Watermelon (Charleston Gray)cgywcgB265
Cucsa.158780Watermelon (Charleston Gray)cgywcgB268
Cucsa.158780Watermelon (97103) v1cgywmB280
Cucsa.158780Watermelon (97103) v1cgywmB283