Cucsa.158730 (gene) Cucumber (Gy14) v1

Overview
NameCucsa.158730
Typegene
OrganismCucumis sativus (Cucumber (Gy14) v1)
DescriptionMatrix metalloproteinase
Locationscaffold01142 : 830789 .. 831670 (+)
Genome Viewer
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGACGTTCAAGTCTCTCGGATTGCTCTTCCTTGTTCTTGTCACCATTGCATCTCACGTAACCTCCAAACACAACCCAATTCTATCTCCTCAACGTCTCTATGGATGTCGTAAAGGCGACAACGTAGAAGGAATCCACAACATTAAAAAATACCTTCAACGTTATGGTTACTTATCACACAACACTGATTCCCATATCATTGAACTCAACAGCAACAAGTTCGACGACTCCCTAGAATCTGCCATTAAATTATATCAGAAATGGTCTCACCTCAACGTAAGTGGGATTCTAGACCAAGAAACATTAGATCAAATGTTCCAACCACGATGTGGCGTACGAGATGTCTTCAAATTTAACTCAAGTAAGAATCTTGAGGATGATCTTGAAATGTCATCTCATTACGCTTTATTTCCAGGCAACCTAAAATGGCCAGATTACAAACGCCACCTAACCTACGTATTTACCAACAATTTCCCAATTAACTTTGTGCCATCAGTGACTGAGGCTATGGCTCGATGGGCAGCCCATTCACTATTCACATTCTCAGAAGCTTCGGATGCCCAAAGTGCTGACATTAATATAAGCTTTCAAATAAAGGATCATGCAGATGGGATGCCTTTTGATGGACCTGGAGGAGTTGTGGGTCATGCTTTTGCACCAATTGATGGAAGATTACACTTAGATGGCGACGATAGTTGGTCGGCAGGGTTGGAAGAGAAGAAGGTTAATGTGATGAATGCGGCACTTCATGAGCTTGGGCATGTTCTTGGTCTTGCCCATAGCACCCTTCCACAAGCTGTCATGTGGCCTTATATTGAATCCAATGCTTTAAAGAACTTGAATGATGATGATATTGCGGGACTTCATGCTTTGTACCCTTAA

mRNA sequence

ATGACGTTCAAGTCTCTCGGATTGCTCTTCCTTGTTCTTGTCACCATTGCATCTCACGTAACCTCCAAACACAACCCAATTCTATCTCCTCAACGTCTCTATGGATGTCGTAAAGGCGACAACGTAGAAGGAATCCACAACATTAAAAAATACCTTCAACGTTATGGTTACTTATCACACAACACTGATTCCCATATCATTGAACTCAACAGCAACAAGTTCGACGACTCCCTAGAATCTGCCATTAAATTATATCAGAAATGGTCTCACCTCAACGTAAGTGGGATTCTAGACCAAGAAACATTAGATCAAATGTTCCAACCACGATGTGGCGTACGAGATGTCTTCAAATTTAACTCAAGTAAGAATCTTGAGGATGATCTTGAAATGTCATCTCATTACGCTTTATTTCCAGGCAACCTAAAATGGCCAGATTACAAACGCCACCTAACCTACGTATTTACCAACAATTTCCCAATTAACTTTGTGCCATCAGTGACTGAGGCTATGGCTCGATGGGCAGCCCATTCACTATTCACATTCTCAGAAGCTTCGGATGCCCAAAGTGCTGACATTAATATAAGCTTTCAAATAAAGGATCATGCAGATGGGATGCCTTTTGATGGACCTGGAGGAGTTGTGGGTCATGCTTTTGCACCAATTGATGGAAGATTACACTTAGATGGCGACGATAGTTGGTCGGCAGGGTTGGAAGAGAAGAAGGTTAATGTGATGAATGCGGCACTTCATGAGCTTGGGCATGTTCTTGGTCTTGCCCATAGCACCCTTCCACAAGCTGTCATGTGGCCTTATATTGAATCCAATGCTTTAAAGAACTTGAATGATGATGATATTGCGGGACTTCATGCTTTGTACCCTTAA

Coding sequence (CDS)

ATGACGTTCAAGTCTCTCGGATTGCTCTTCCTTGTTCTTGTCACCATTGCATCTCACGTAACCTCCAAACACAACCCAATTCTATCTCCTCAACGTCTCTATGGATGTCGTAAAGGCGACAACGTAGAAGGAATCCACAACATTAAAAAATACCTTCAACGTTATGGTTACTTATCACACAACACTGATTCCCATATCATTGAACTCAACAGCAACAAGTTCGACGACTCCCTAGAATCTGCCATTAAATTATATCAGAAATGGTCTCACCTCAACGTAAGTGGGATTCTAGACCAAGAAACATTAGATCAAATGTTCCAACCACGATGTGGCGTACGAGATGTCTTCAAATTTAACTCAAGTAAGAATCTTGAGGATGATCTTGAAATGTCATCTCATTACGCTTTATTTCCAGGCAACCTAAAATGGCCAGATTACAAACGCCACCTAACCTACGTATTTACCAACAATTTCCCAATTAACTTTGTGCCATCAGTGACTGAGGCTATGGCTCGATGGGCAGCCCATTCACTATTCACATTCTCAGAAGCTTCGGATGCCCAAAGTGCTGACATTAATATAAGCTTTCAAATAAAGGATCATGCAGATGGGATGCCTTTTGATGGACCTGGAGGAGTTGTGGGTCATGCTTTTGCACCAATTGATGGAAGATTACACTTAGATGGCGACGATAGTTGGTCGGCAGGGTTGGAAGAGAAGAAGGTTAATGTGATGAATGCGGCACTTCATGAGCTTGGGCATGTTCTTGGTCTTGCCCATAGCACCCTTCCACAAGCTGTCATGTGGCCTTATATTGAATCCAATGCTTTAAAGAACTTGAATGATGATGATATTGCGGGACTTCATGCTTTGTACCCTTAA

Protein sequence

MTFKSLGLLFLVLVTIASHVTSKHNPILSPQRLYGCRKGDNVEGIHNIKKYLQRYGYLSHNTDSHIIELNSNKFDDSLESAIKLYQKWSHLNVSGILDQETLDQMFQPRCGVRDVFKFNSSKNLEDDLEMSSHYALFPGNLKWPDYKRHLTYVFTNNFPINFVPSVTEAMARWAAHSLFTFSEASDAQSADINISFQIKDHADGMPFDGPGGVVGHAFAPIDGRLHLDGDDSWSAGLEEKKVNVMNAALHELGHVLGLAHSTLPQAVMWPYIESNALKNLNDDDIAGLHALYP*
Homology
[Close]
BLAST of Cucsa.158730 vs. Swiss-Prot
Match: 2MMP_ARATH (Metalloendoproteinase 2-MMP OS=Arabidopsis thaliana GN=2MMP PE=1 SV=1)

HSP 1 Score: 182.2 bits (461), Expect = 8.3e-45
Identity = 105/282 (37.23%), Postives = 156/282 (55.32%), Query Frame = 1 

Query: 35  GCRKGDNVEGIHNIKKYLQRYGYLSHNTDSHIIELNSNKFDDSLESAIKLYQKWSHLNVS 94
           GC  G NV+G++ IKKY QR+GY+      +     ++ FDD L++A++LYQ   +LNV+
Sbjct: 49  GCHHGQNVDGLYRIKKYFQRFGYIPETFSGNF----TDDFDDILKAAVELYQTNFNLNVT 108

Query: 95  GILDQETLDQMFQPRCGVRDV--------------FKFNSSKNLEDDLEMSSHYALFPGN 154
           G LD  T+  +  PRCG  DV              F+ N S+     L     Y LFPG 
Sbjct: 109 GELDALTIQHIVIPRCGNPDVVNGTSLMHGGRRKTFEVNFSRT---HLHAVKRYTLFPGE 168

Query: 155 LKWPDYKRHLTYVFTNNFPINF-VPSV-TEAMARWAAHSLFTFSEASDAQSADINISFQI 214
            +WP  +R LTY F    P+   V SV + A  RW+  +   F+ +    ++DI I F  
Sbjct: 169 PRWPRNRRDLTYAFDPKNPLTEEVKSVFSRAFGRWSDVTALNFTLSESFSTSDITIGFYT 228

Query: 215 KDHADGMPFDGPGGVVGHAFAPIDGRLHLDGDDSW--SAGLEE-----KKVNVMNAALHE 274
            DH DG PFDG  G + HAF+P  G+ HLD D++W  S  L+        V++ + A+HE
Sbjct: 229 GDHGDGEPFDGVLGTLAHAFSPPSGKFHLDADENWVVSGDLDSFLSVTAAVDLESVAVHE 288

Query: 275 LGHVLGLAHSTLPQAVMWPYIESNALK-NLNDDDIAGLHALY 293
           +GH+LGL HS++ +++M+P I +   K +L +DD+ G+  LY
Sbjct: 289 IGHLLGLGHSSVEESIMYPTITTGKRKVDLTNDDVEGIQYLY 323

[Close]
BLAST of Cucsa.158730 vs. Swiss-Prot
Match: 5MMP_ARATH (Metalloendoproteinase 5-MMP OS=Arabidopsis thaliana GN=5MMP PE=1 SV=1)

HSP 1 Score: 181.8 bits (460), Expect = 1.1e-44
Identity = 105/273 (38.46%), Postives = 148/273 (54.21%), Query Frame = 1 

Query: 32  RLYGCRKGDNVEGIHNIKKYLQRYGYLSHNTDSHIIELNSNKFDDSLESAIKLYQKWSHL 91
           +L GC  G+N+ G+  +K+Y +R+GY++   +       ++ FDD L+SAI  YQK  +L
Sbjct: 47  KLAGCHIGENINGLSKLKQYFRRFGYITTTGNC------TDDFDDVLQSAINTYQKNFNL 106

Query: 92  NVSGILDQETLDQMFQPRCGVRDVFKFNSSKNLEDDLEMSSHYALFPGNLKWPDYKRHLT 151
            V+G LD  TL Q+ +PRCG  D+    S  N    L  +  Y+ FPG  +WP  KR LT
Sbjct: 107 KVTGKLDSSTLRQIVKPRCGNPDLIDGVSEMNGGKILRTTEKYSFFPGKPRWPKRKRDLT 166

Query: 152 YVFT--NNFPINFVPSVTEAMARWAAHSLFTFSEASDAQSADINISFQIKDHADGMPFDG 211
           Y F   NN         + A  RWA  +   F+ +     ADI I F   +H DG PFDG
Sbjct: 167 YAFAPQNNLTDEVKRVFSRAFTRWAEVTPLNFTRSESILRADIVIGFFSGEHGDGEPFDG 226

Query: 212 PGGVVGHAFAPIDGRLHLDGDDSW--SAGLEEKK-------VNVMNAALHELGHVLGLAH 271
             G + HA +P  G LHLDGD+ W  S G   ++       V++ + A+HE+GH+LGL H
Sbjct: 227 AMGTLAHASSPPTGMLHLDGDEDWLISNGEISRRILPVTTVVDLESVAVHEIGHLLGLGH 286

Query: 272 STLPQAVMWPYIESNALK-NLNDDDIAGLHALY 293
           S++  A+M+P I     K  L  DDI G+  LY
Sbjct: 287 SSVEDAIMFPAISGGDRKVELAKDDIEGIQHLY 313

[Close]
BLAST of Cucsa.158730 vs. Swiss-Prot
Match: MEP1_SOYBN (Metalloendoproteinase 1 OS=Glycine max PE=1 SV=2)

HSP 1 Score: 176.4 bits (446), Expect = 4.6e-43
Identity = 103/263 (39.16%), Postives = 142/263 (53.99%), Query Frame = 1 

Query: 39  GDNVEGIHNIKKYLQRYGYLSHNTDSHIIELNSNKFDDSLESAIKLYQKWSHLNVSGILD 98
           G N +G+ N+K Y    GY+ +           + FDD+L SAIK YQK  +LNV+G  D
Sbjct: 48  GQNYKGLSNVKNYFHHLGYIPNAPHF------DDNFDDTLVSAIKTYQKNYNLNVTGKFD 107

Query: 99  QETLDQMFQPRCGVRDVFKFNSSKNLEDDLEMSSHYALFPGNLKWPDYKRHLTYVFTNNF 158
             TL Q+  PRCGV D+   N++K       M S Y  F    +W      LTY F+   
Sbjct: 108 INTLKQIMTPRCGVPDII-INTNKTTS--FGMISDYTFFKDMPRWQAGTTQLTYAFSPEP 167

Query: 159 PIN--FVPSVTEAMARWAAHSLFTFSEASDAQSADINISFQIKDHADGMPFDGPGGVVGH 218
            ++  F  ++  A ++W       F E +  ++A+I I F  K+H D  PFDGPGG++GH
Sbjct: 168 RLDDTFKSAIARAFSKWTPVVNIAFQETTSYETANIKILFASKNHGDPYPFDGPGGILGH 227

Query: 219 AFAPIDGRLHLDGDDSWSAGLEEKKVNVMNA------ALHELGHVLGLAHSTLPQAVMWP 278
           AFAP DGR H D D+ W A  +  K  V +A      A+HE+GH+LGL HS+  +A+M+P
Sbjct: 228 AFAPTDGRCHFDADEYWVASGDVTKSPVTSAFDLESVAVHEIGHLLGLGHSSDLRAIMYP 287

Query: 279 YIESNALK-NLNDDDIAGLHALY 293
            I     K NL  DDI G+  LY
Sbjct: 288 SIPPRTRKVNLAQDDIDGIRKLY 301

[Close]
BLAST of Cucsa.158730 vs. Swiss-Prot
Match: 3MMP_ARATH (Metalloendoproteinase 3-MMP OS=Arabidopsis thaliana GN=3MMP PE=1 SV=1)

HSP 1 Score: 176.0 bits (445), Expect = 6.0e-43
Identity = 102/291 (35.05%), Postives = 159/291 (54.64%), Query Frame = 1 

Query: 25  NPILSPQRLYGCRKGDNVEGIHNIKKYLQRYGYLSHNTDSHIIELNSNKFDDSLESAIKL 84
           NP  S     GC  G   +G++ +K+Y Q +GY+   T++++    ++ FDD L++A+++
Sbjct: 41  NPWNSFLNFTGCHAGKKYDGLYMLKQYFQHFGYI---TETNLSGNFTDDFDDILKNAVEM 100

Query: 85  YQKWSHLNVSGILDQETLDQMFQPRCGVRDVFKFNSSKNL-EDDLEMS-----------S 144
           YQ+   LNV+G+LD+ TL  +  PRCG  DV    S+ +      E+S            
Sbjct: 101 YQRNFQLNVTGVLDELTLKHVVIPRCGNPDVVNGTSTMHSGRKTFEVSFAGRGQRFHAVK 160

Query: 145 HYALFPGNLKWPDYKRHLTYVFT-NNFPINFVPSV-TEAMARWAAHSLFTFSEASDAQSA 204
           HY+ FPG  +WP  +R LTY F   N     V SV + A  RW   +  TF+      ++
Sbjct: 161 HYSFFPGEPRWPRNRRDLTYAFDPRNALTEEVKSVFSRAFTRWEEVTPLTFTRVERFSTS 220

Query: 205 DINISFQIKDHADGMPFDGPGGVVGHAFAPIDGRLHLDGDDSWSAGLE--------EKKV 264
           DI+I F   +H DG PFDGP   + HAF+P  G  HLDG+++W    E         + V
Sbjct: 221 DISIGFYSGEHGDGEPFDGPMRTLAHAFSPPTGHFHLDGEENWIVSGEGGDGFISVSEAV 280

Query: 265 NVMNAALHELGHVLGLAHSTLPQAVMWPYIESNALK-NLNDDDIAGLHALY 293
           ++ + A+HE+GH+LGL HS++  ++M+P I +   K +L  DD+ G+  LY
Sbjct: 281 DLESVAVHEIGHLLGLGHSSVEGSIMYPTIRTGRRKVDLTTDDVEGVQYLY 328

[Close]
BLAST of Cucsa.158730 vs. Swiss-Prot
Match: 1MMP_ARATH (Metalloendoproteinase 1-MMP OS=Arabidopsis thaliana GN=1MMP PE=1 SV=1)

HSP 1 Score: 172.6 bits (436), Expect = 6.6e-42
Identity = 102/273 (37.36%), Postives = 150/273 (54.95%), Query Frame = 1 

Query: 32  RLYGCRKGDNVEGIHNIKKYLQRYGYLSHNTDSHIIELNSNKFDDSLESAIKLYQKWSHL 91
           RL   + G +V G+  +K+YL R+GY++  +     E+ S+ FD  LESAI LYQ+   L
Sbjct: 57  RLVDVQIGSHVSGVSELKRYLHRFGYVNDGS-----EIFSDVFDGPLESAISLYQENLGL 116

Query: 92  NVSGILDQETLDQMFQPRCGVRDVFKFNSSKNLEDDLEMSSHYALFPGNLKWPDYKRHLT 151
            ++G LD  T+  M  PRCGV D     ++    D L  ++HY  F G  KW   +  LT
Sbjct: 117 PITGRLDTSTVTLMSLPRCGVSDTHMTINN----DFLHTTAHYTYFNGKPKWN--RDTLT 176

Query: 152 YVFTNNFPINFVPS------VTEAMARWAAHSLFTFSEASDAQSADINISFQIKDHADGM 211
           Y  +    ++++ S         A ++W++    +F E  D  +AD+ I F   DH DG+
Sbjct: 177 YAISKTHKLDYLTSEDVKTVFRRAFSQWSSVIPVSFEEVDDFTTADLKIGFYAGDHGDGL 236

Query: 212 PFDGPGGVVGHAFAPIDGRLHLDGDDSWSA-----GLEEKKVNVMNAALHELGHVLGLAH 271
           PFDG  G + HAFAP +GRLHLD  ++W       G  E  V++ + A HE+GH+LGL H
Sbjct: 237 PFDGVLGTLAHAFAPENGRLHLDAAETWIVDDDLKGSSEVAVDLESVATHEIGHLLGLGH 296

Query: 272 STLPQAVMWPYIESNALK-NLNDDDIAGLHALY 293
           S+   AVM+P +     K +L  DD+AG+  LY
Sbjct: 297 SSQESAVMYPSLRPRTKKVDLTVDDVAGVLKLY 318

[Close]
BLAST of Cucsa.158730 vs. TrEMBL
Match: A0A0A0KNY8_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_5G522910 PE=3 SV=1)

HSP 1 Score: 571.2 bits (1471), Expect = 7.1e-160
Identity = 275/295 (93.22%), Postives = 286/295 (96.95%), Query Frame = 1 

Query: 1   MTFKSLGLLFLVLVTIASHVTSKHNPILSPQRLYGCRKGDNVEGIHNIKKYLQRYGYLSH 60
           M FKSL LLFLVLVTIASHV+SKHNPILSPQRLYGCRKGDNVEGIH+IKKYLQRYGYLSH
Sbjct: 1   MAFKSLQLLFLVLVTIASHVSSKHNPILSPQRLYGCRKGDNVEGIHSIKKYLQRYGYLSH 60

Query: 61  NT--DSHIIELNSNKFDDSLESAIKLYQKWSHLNVSGILDQETLDQMFQPRCGVRDVFKF 120
           NT  DSHIIELNSNKFDDSLESAIKLYQKWSHLNVSGILDQETLDQMF+PRCGVRDVFKF
Sbjct: 61  NTSIDSHIIELNSNKFDDSLESAIKLYQKWSHLNVSGILDQETLDQMFKPRCGVRDVFKF 120

Query: 121 NSSKNLEDDLEMSSHYALFPGNLKWPDYKRHLTYVFTNNFPINFVPSVTEAMARWAAHSL 180
           NS+KNLEDDLEMSSHY LFPGNLKWPDYKRHLTYVFTNNFP++FVPSVTEAMARWAA SL
Sbjct: 121 NSNKNLEDDLEMSSHYTLFPGNLKWPDYKRHLTYVFTNNFPVDFVPSVTEAMARWAAQSL 180

Query: 181 FTFSEASDAQSADINISFQIKDHADGMPFDGPGGVVGHAFAPIDGRLHLDGDDSWSAGLE 240
           FTFSEASDAQSADINISFQIKDHADG+PFDGPGGVVGHAFAP DGRLHLDGDD+WSAG+E
Sbjct: 181 FTFSEASDAQSADINISFQIKDHADGLPFDGPGGVVGHAFAPTDGRLHLDGDDTWSAGME 240

Query: 241 EKKVNVMNAALHELGHVLGLAHSTLPQAVMWPYIESNALKNLNDDDIAGLHALYP 294
            +KVNVMN ALHELGHVLGLAHSTLPQAVMWPYI+SNALKNLNDDDIAGLHALYP
Sbjct: 241 VQKVNVMNVALHELGHVLGLAHSTLPQAVMWPYIDSNALKNLNDDDIAGLHALYP 295

[Close]
BLAST of Cucsa.158730 vs. TrEMBL
Match: A0A0A0KX71_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_5G631560 PE=3 SV=1)

HSP 1 Score: 552.4 bits (1422), Expect = 3.4e-154
Identity = 267/295 (90.51%), Postives = 280/295 (94.92%), Query Frame = 1 

Query: 1   MTFKSLGLLFLVLVTIASHVTSKHNPILSPQRLYGCRKGDNVEGIHNIKKYLQRYGYLSH 60
           M FKSL LLFL+L +IASHV SKHN ILSP+RLYGCRK DNVEGIH+IKKYLQRYGYLSH
Sbjct: 1   MAFKSLQLLFLILASIASHVISKHNSILSPRRLYGCRKDDNVEGIHSIKKYLQRYGYLSH 60

Query: 61  NT--DSHIIELNSNKFDDSLESAIKLYQKWSHLNVSGILDQETLDQMFQPRCGVRDVFKF 120
           NT  DS+IIELNSNKFDDSLESAI+LYQKWSHLNVSGI+DQETLDQMFQPRCGV DVFKF
Sbjct: 61  NTSTDSNIIELNSNKFDDSLESAIRLYQKWSHLNVSGIIDQETLDQMFQPRCGVPDVFKF 120

Query: 121 NSSKNLEDDLEMSSHYALFPGNLKWPDYKRHLTYVFTNNFPINFVPSVTEAMARWAAHSL 180
           NSSKNLEDDLEMSSHYALFP N KWPDYKRHLTY+FTNNFPI+FVPS+TEAMARWAA SL
Sbjct: 121 NSSKNLEDDLEMSSHYALFPNNEKWPDYKRHLTYMFTNNFPIDFVPSMTEAMARWAAQSL 180

Query: 181 FTFSEASDAQSADINISFQIKDHADGMPFDGPGGVVGHAFAPIDGRLHLDGDDSWSAGLE 240
           FTFSEASDAQSADINISFQ+KDHADG  FDGPGGVVGHAFAP DGRLHLDGDD+WSAGLE
Sbjct: 181 FTFSEASDAQSADINISFQVKDHADGFAFDGPGGVVGHAFAPTDGRLHLDGDDTWSAGLE 240

Query: 241 EKKVNVMNAALHELGHVLGLAHSTLPQAVMWPYIESNALKNLNDDDIAGLHALYP 294
           EKKVNVMNAALHELGHVLGLAHSTLPQAVMWPYIESNALKNLNDDDIAG+HALYP
Sbjct: 241 EKKVNVMNAALHELGHVLGLAHSTLPQAVMWPYIESNALKNLNDDDIAGIHALYP 295

[Close]
BLAST of Cucsa.158730 vs. TrEMBL
Match: A0A0A0KVD8_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_5G633150 PE=3 SV=1)

HSP 1 Score: 538.5 bits (1386), Expect = 5.1e-150
Identity = 262/292 (89.73%), Postives = 273/292 (93.49%), Query Frame = 1 

Query: 4   KSLGLLFLVLVTIASHVTSKHNPILSPQRLYGCRKGDNVEGIHNIKKYLQRYGYLSHNT- 63
           KSL LLFLVL +IASHV S +N ILSP+RL GCRKGDNVEGIHNIKKYLQRYGYLSHNT 
Sbjct: 3   KSLQLLFLVLASIASHVISINNQILSPRRLNGCRKGDNVEGIHNIKKYLQRYGYLSHNTS 62

Query: 64  -DSHIIELNSNKFDDSLESAIKLYQKWSHLNVSGILDQETLDQMFQPRCGVRDVFKFNSS 123
            DS+IIELNSNKFDDSLESAI+LYQKWSHLNVSGILDQETLDQMFQ RCG+RDVFKFNSS
Sbjct: 63  TDSNIIELNSNKFDDSLESAIRLYQKWSHLNVSGILDQETLDQMFQQRCGIRDVFKFNSS 122

Query: 124 KNLEDDLEMSSHYALFPGNLKWPDYKRHLTYVFTNNFPINFVPSVTEAMARWAAHSLFTF 183
           KNLEDDLEMSSHY LFP N KWPDYKRHLTY+FTNNFP++FVPSVTEAMARWAA SLFTF
Sbjct: 123 KNLEDDLEMSSHYVLFPNNKKWPDYKRHLTYMFTNNFPVDFVPSVTEAMARWAAQSLFTF 182

Query: 184 SEASDAQSADINISFQIKDHADGMPFDGPGGVVGHAFAPIDGRLHLDGDDSWSAGLEEKK 243
           SEASDAQSADINISFQ+KDHADG  FDGPGGVVGHAFAP DGRLHLDGDDSWSAGLEE K
Sbjct: 183 SEASDAQSADINISFQVKDHADGSAFDGPGGVVGHAFAPTDGRLHLDGDDSWSAGLEENK 242

Query: 244 VNVMNAALHELGHVLGLAHSTLPQAVMWPYIESNALKNLNDDDIAGLHALYP 294
            NVMN ALHELGHVLGLAHSTLPQAVMWPYI+SNALKNLNDDDIAGLHALYP
Sbjct: 243 FNVMNVALHELGHVLGLAHSTLPQAVMWPYIDSNALKNLNDDDIAGLHALYP 294

[Close]
BLAST of Cucsa.158730 vs. TrEMBL
Match: A0A0A0KS35_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_5G632070 PE=3 SV=1)

HSP 1 Score: 270.8 bits (691), Expect = 2.0e-69
Identity = 144/298 (48.32%), Postives = 192/298 (64.43%), Query Frame = 1 

Query: 9   LFLVLVTIASHVTS-------KHNPILSPQRLYGCRKGDN-VEGIHNIKKYLQRYGYLSH 68
           LFL+L  +  H+TS       K + +L P+ L G  K D+ VEGIH +K YLQRYGY S+
Sbjct: 9   LFLLLAFVTPHLTSARPLHNHKSSRLLFPEHLIGSSKNDSIVEGIHKVKAYLQRYGYFSN 68

Query: 69  NTDSHIIELNSNKFDDSLESAIKLYQKWSHLNVSGILDQETLDQMFQPRCGVRDVFKFNS 128
             +++   L+++ FDD LESAIK YQK+S+L VSG+LD+ETL QM +PRCGV D F+  +
Sbjct: 69  ENENN---LSTDAFDDDLESAIKSYQKFSNLKVSGVLDRETLQQMSRPRCGVADNFQSVA 128

Query: 129 SKNLEDDLEM----SSHYALFPGNLKWPDYKRHLTYVFTNNFPINFVPSVTEAMARWAAH 188
            ++ E++        SH+  FPG  KWP  K HLTY F +N+P+    +V  A  +WAA+
Sbjct: 129 QQDGENNNTTVQIGGSHFMFFPGKGKWPYRKWHLTYGFVHNYPMKHAAAVVRAFDKWAAN 188

Query: 189 SLFTFSEASDAQSADINISFQIKDHADGMPFDGPGGVVGHAFAPIDGRLHLDGDDSWS-- 248
           S FTFS A   Q+ADI +SF+  DH DG PFDG GG++GHAF PIDGR+H D D+ W+  
Sbjct: 189 SKFTFSLAWRIQTADILLSFERGDHGDGKPFDGEGGILGHAFGPIDGRVHFDADEQWAEI 248

Query: 249 AGLEEKKVNVMNAALHELGHVLGLAHSTLPQAVMWPYIESNALK-NLNDDDIAGLHAL 292
             L  +  +  + ALHE+GH LGL HS  P AVMW  +E+   K  L  DDI G+HAL
Sbjct: 249 GSLTNENFDFESVALHEIGHALGLGHSIFPSAVMWANMETGVNKTELTIDDIEGVHAL 303

[Close]
BLAST of Cucsa.158730 vs. TrEMBL
Match: A0A0A0KU02_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_5G632080 PE=3 SV=1)

HSP 1 Score: 268.5 bits (685), Expect = 9.8e-69
Identity = 141/293 (48.12%), Postives = 193/293 (65.87%), Query Frame = 1 

Query: 9   LFLVLVTIASHVTS---KHNPILSPQRLYGCRKGDNVEGIHNIKKYLQRYGYLSHNTDSH 68
           LFL++ +IA HV +   +H  I S   L G R G+N+EGIHN+K YL+RYGYL++   ++
Sbjct: 9   LFLLIASIAPHVITARVRHTHISS--HLQGSRIGNNIEGIHNVKLYLKRYGYLTNVESTN 68

Query: 69  IIELNSNKFDDSLESAIKLYQKWSHLNVSGILDQETLDQMFQPRCGVRDVFKFNSSKNLE 128
               ++N FD  LESAIK +QK+  LNVSG++D+ETL  M  PRCG+ D+   ++   ++
Sbjct: 69  ----SNNTFDVLLESAIKTFQKYHSLNVSGVIDEETLTLMSLPRCGIPDIM--HNINVMD 128

Query: 129 DDLEMSS-----HYALFPGNLKWPDYKRHLTYVFTNNFPINFVPSVTEAMARWAAHSLFT 188
           ++++M+S     H+  FP NLKWP  K +L Y F ++FP +F   V  AM +WA  SLF 
Sbjct: 129 NNVQMNSSSFHSHFTFFPDNLKWPVSKYNLRYTFLDDFPNDFKEPVMNAMEQWALFSLFR 188

Query: 189 FSEASDAQSADINISFQIKDHADGMPFDGPGGVVGHAFAPIDGRLHLDGDDSWSAGLEEK 248
           FSEA +AQ ADI  +F   +H DG PFDG GGV+ HAF P+DGR+H D D+ W+ G    
Sbjct: 189 FSEAVEAQEADITFNFVRGNHGDGYPFDGKGGVLAHAFGPLDGRVHFDWDEDWADGSVGG 248

Query: 249 KVNVMNAALHELGHVLGLAHSTLPQAVMWPYIES-NALKNLNDDDIAGLHALY 293
            +NV   ALHELGHVLGLAHST+  A+MWPY+E+    + L  DDI G+  LY
Sbjct: 249 FINVGMVALHELGHVLGLAHSTIRDAIMWPYMEAGEQTRGLQFDDIEGIQTLY 293

[Close]
BLAST of Cucsa.158730 vs. TAIR10
Match: AT1G70170.1 (AT1G70170.1 matrix metalloproteinase)

HSP 1 Score: 182.2 bits (461), Expect = 4.7e-46
Identity = 105/282 (37.23%), Postives = 156/282 (55.32%), Query Frame = 1 

Query: 35  GCRKGDNVEGIHNIKKYLQRYGYLSHNTDSHIIELNSNKFDDSLESAIKLYQKWSHLNVS 94
           GC  G NV+G++ IKKY QR+GY+      +     ++ FDD L++A++LYQ   +LNV+
Sbjct: 49  GCHHGQNVDGLYRIKKYFQRFGYIPETFSGNF----TDDFDDILKAAVELYQTNFNLNVT 108

Query: 95  GILDQETLDQMFQPRCGVRDV--------------FKFNSSKNLEDDLEMSSHYALFPGN 154
           G LD  T+  +  PRCG  DV              F+ N S+     L     Y LFPG 
Sbjct: 109 GELDALTIQHIVIPRCGNPDVVNGTSLMHGGRRKTFEVNFSRT---HLHAVKRYTLFPGE 168

Query: 155 LKWPDYKRHLTYVFTNNFPINF-VPSV-TEAMARWAAHSLFTFSEASDAQSADINISFQI 214
            +WP  +R LTY F    P+   V SV + A  RW+  +   F+ +    ++DI I F  
Sbjct: 169 PRWPRNRRDLTYAFDPKNPLTEEVKSVFSRAFGRWSDVTALNFTLSESFSTSDITIGFYT 228

Query: 215 KDHADGMPFDGPGGVVGHAFAPIDGRLHLDGDDSW--SAGLEE-----KKVNVMNAALHE 274
            DH DG PFDG  G + HAF+P  G+ HLD D++W  S  L+        V++ + A+HE
Sbjct: 229 GDHGDGEPFDGVLGTLAHAFSPPSGKFHLDADENWVVSGDLDSFLSVTAAVDLESVAVHE 288

Query: 275 LGHVLGLAHSTLPQAVMWPYIESNALK-NLNDDDIAGLHALY 293
           +GH+LGL HS++ +++M+P I +   K +L +DD+ G+  LY
Sbjct: 289 IGHLLGLGHSSVEESIMYPTITTGKRKVDLTNDDVEGIQYLY 323

[Close]
BLAST of Cucsa.158730 vs. TAIR10
Match: AT1G59970.1 (AT1G59970.1 Matrixin family protein)

HSP 1 Score: 181.8 bits (460), Expect = 6.1e-46
Identity = 105/273 (38.46%), Postives = 148/273 (54.21%), Query Frame = 1 

Query: 32  RLYGCRKGDNVEGIHNIKKYLQRYGYLSHNTDSHIIELNSNKFDDSLESAIKLYQKWSHL 91
           +L GC  G+N+ G+  +K+Y +R+GY++   +       ++ FDD L+SAI  YQK  +L
Sbjct: 47  KLAGCHIGENINGLSKLKQYFRRFGYITTTGNC------TDDFDDVLQSAINTYQKNFNL 106

Query: 92  NVSGILDQETLDQMFQPRCGVRDVFKFNSSKNLEDDLEMSSHYALFPGNLKWPDYKRHLT 151
            V+G LD  TL Q+ +PRCG  D+    S  N    L  +  Y+ FPG  +WP  KR LT
Sbjct: 107 KVTGKLDSSTLRQIVKPRCGNPDLIDGVSEMNGGKILRTTEKYSFFPGKPRWPKRKRDLT 166

Query: 152 YVFT--NNFPINFVPSVTEAMARWAAHSLFTFSEASDAQSADINISFQIKDHADGMPFDG 211
           Y F   NN         + A  RWA  +   F+ +     ADI I F   +H DG PFDG
Sbjct: 167 YAFAPQNNLTDEVKRVFSRAFTRWAEVTPLNFTRSESILRADIVIGFFSGEHGDGEPFDG 226

Query: 212 PGGVVGHAFAPIDGRLHLDGDDSW--SAGLEEKK-------VNVMNAALHELGHVLGLAH 271
             G + HA +P  G LHLDGD+ W  S G   ++       V++ + A+HE+GH+LGL H
Sbjct: 227 AMGTLAHASSPPTGMLHLDGDEDWLISNGEISRRILPVTTVVDLESVAVHEIGHLLGLGH 286

Query: 272 STLPQAVMWPYIESNALK-NLNDDDIAGLHALY 293
           S++  A+M+P I     K  L  DDI G+  LY
Sbjct: 287 SSVEDAIMFPAISGGDRKVELAKDDIEGIQHLY 313

[Close]
BLAST of Cucsa.158730 vs. TAIR10
Match: AT1G24140.1 (AT1G24140.1 Matrixin family protein)

HSP 1 Score: 176.0 bits (445), Expect = 3.4e-44
Identity = 102/291 (35.05%), Postives = 159/291 (54.64%), Query Frame = 1 

Query: 25  NPILSPQRLYGCRKGDNVEGIHNIKKYLQRYGYLSHNTDSHIIELNSNKFDDSLESAIKL 84
           NP  S     GC  G   +G++ +K+Y Q +GY+   T++++    ++ FDD L++A+++
Sbjct: 41  NPWNSFLNFTGCHAGKKYDGLYMLKQYFQHFGYI---TETNLSGNFTDDFDDILKNAVEM 100

Query: 85  YQKWSHLNVSGILDQETLDQMFQPRCGVRDVFKFNSSKNL-EDDLEMS-----------S 144
           YQ+   LNV+G+LD+ TL  +  PRCG  DV    S+ +      E+S            
Sbjct: 101 YQRNFQLNVTGVLDELTLKHVVIPRCGNPDVVNGTSTMHSGRKTFEVSFAGRGQRFHAVK 160

Query: 145 HYALFPGNLKWPDYKRHLTYVFT-NNFPINFVPSV-TEAMARWAAHSLFTFSEASDAQSA 204
           HY+ FPG  +WP  +R LTY F   N     V SV + A  RW   +  TF+      ++
Sbjct: 161 HYSFFPGEPRWPRNRRDLTYAFDPRNALTEEVKSVFSRAFTRWEEVTPLTFTRVERFSTS 220

Query: 205 DINISFQIKDHADGMPFDGPGGVVGHAFAPIDGRLHLDGDDSWSAGLE--------EKKV 264
           DI+I F   +H DG PFDGP   + HAF+P  G  HLDG+++W    E         + V
Sbjct: 221 DISIGFYSGEHGDGEPFDGPMRTLAHAFSPPTGHFHLDGEENWIVSGEGGDGFISVSEAV 280

Query: 265 NVMNAALHELGHVLGLAHSTLPQAVMWPYIESNALK-NLNDDDIAGLHALY 293
           ++ + A+HE+GH+LGL HS++  ++M+P I +   K +L  DD+ G+  LY
Sbjct: 281 DLESVAVHEIGHLLGLGHSSVEGSIMYPTIRTGRRKVDLTTDDVEGVQYLY 328

[Close]
BLAST of Cucsa.158730 vs. TAIR10
Match: AT4G16640.1 (AT4G16640.1 Matrixin family protein)

HSP 1 Score: 172.6 bits (436), Expect = 3.7e-43
Identity = 102/273 (37.36%), Postives = 150/273 (54.95%), Query Frame = 1 

Query: 32  RLYGCRKGDNVEGIHNIKKYLQRYGYLSHNTDSHIIELNSNKFDDSLESAIKLYQKWSHL 91
           RL   + G +V G+  +K+YL R+GY++  +     E+ S+ FD  LESAI LYQ+   L
Sbjct: 57  RLVDVQIGSHVSGVSELKRYLHRFGYVNDGS-----EIFSDVFDGPLESAISLYQENLGL 116

Query: 92  NVSGILDQETLDQMFQPRCGVRDVFKFNSSKNLEDDLEMSSHYALFPGNLKWPDYKRHLT 151
            ++G LD  T+  M  PRCGV D     ++    D L  ++HY  F G  KW   +  LT
Sbjct: 117 PITGRLDTSTVTLMSLPRCGVSDTHMTINN----DFLHTTAHYTYFNGKPKWN--RDTLT 176

Query: 152 YVFTNNFPINFVPS------VTEAMARWAAHSLFTFSEASDAQSADINISFQIKDHADGM 211
           Y  +    ++++ S         A ++W++    +F E  D  +AD+ I F   DH DG+
Sbjct: 177 YAISKTHKLDYLTSEDVKTVFRRAFSQWSSVIPVSFEEVDDFTTADLKIGFYAGDHGDGL 236

Query: 212 PFDGPGGVVGHAFAPIDGRLHLDGDDSWSA-----GLEEKKVNVMNAALHELGHVLGLAH 271
           PFDG  G + HAFAP +GRLHLD  ++W       G  E  V++ + A HE+GH+LGL H
Sbjct: 237 PFDGVLGTLAHAFAPENGRLHLDAAETWIVDDDLKGSSEVAVDLESVATHEIGHLLGLGH 296

Query: 272 STLPQAVMWPYIESNALK-NLNDDDIAGLHALY 293
           S+   AVM+P +     K +L  DD+AG+  LY
Sbjct: 297 SSQESAVMYPSLRPRTKKVDLTVDDVAGVLKLY 318

[Close]
BLAST of Cucsa.158730 vs. TAIR10
Match: AT2G45040.1 (AT2G45040.1 Matrixin family protein)

HSP 1 Score: 158.7 bits (400), Expect = 5.5e-39
Identity = 97/260 (37.31%), Postives = 137/260 (52.69%), Query Frame = 1 

Query: 45  IHNIKKYLQRYGYLSHNTDSHIIELNSNKFDDSLESAIKLYQKWSHLNVSGILDQETLDQ 104
           I  IK++LQ+YGYL  N +S          D S E A+  YQK   L ++G  D +TL Q
Sbjct: 50  IPEIKRHLQQYGYLPQNKESD---------DVSFEQALVRYQKNLGLPITGKPDSDTLSQ 109

Query: 105 MFQPRCGVRDVFKFNSSKNLEDDLEMSSHYALFPGNLKWP-DYKRHLTYVFTNN------ 164
           +  PRCG  D  +  ++            Y  FPG  +W  D    LTY F+        
Sbjct: 110 ILLPRCGFPDDVEPKTAP-----FHTGKKYVYFPGRPRWTRDVPLKLTYAFSQENLTPYL 169

Query: 165 FPINFVPSVTEAMARWAAHSLFTFSEASDAQSADINISFQIKDHADGMPFDGPGGVVGHA 224
            P +       A  +WA+    +F E  D   ADI I F   DH DG PFDG  GV+ H 
Sbjct: 170 APTDIRRVFRRAFGKWASVIPVSFIETEDYVIADIKIGFFNGDHGDGEPFDGVLGVLAHT 229

Query: 225 FAPIDGRLHLDGDDSWSAGLEEKK----VNVMNAALHELGHVLGLAHSTLPQAVMWPYIE 284
           F+P +GRLHLD  ++W+   +E+K    V++ + A+HE+GHVLGL HS++  A M+P ++
Sbjct: 230 FSPENGRLHLDKAETWAVDFDEEKSSVAVDLESVAVHEIGHVLGLGHSSVKDAAMYPTLK 289

Query: 285 SNALK-NLNDDDIAGLHALY 293
             + K NLN DD+ G+ +LY
Sbjct: 290 PRSKKVNLNMDDVVGVQSLY 295

[Close]
BLAST of Cucsa.158730 vs. NCBI nr
Match: gi|778708586|ref|XP_004142843.2| (PREDICTED: metalloendoproteinase 1-like [Cucumis sativus])

HSP 1 Score: 571.2 bits (1471), Expect = 1.0e-159
Identity = 275/295 (93.22%), Postives = 286/295 (96.95%), Query Frame = 1 

Query: 1   MTFKSLGLLFLVLVTIASHVTSKHNPILSPQRLYGCRKGDNVEGIHNIKKYLQRYGYLSH 60
           M FKSL LLFLVLVTIASHV+SKHNPILSPQRLYGCRKGDNVEGIH+IKKYLQRYGYLSH
Sbjct: 1   MAFKSLQLLFLVLVTIASHVSSKHNPILSPQRLYGCRKGDNVEGIHSIKKYLQRYGYLSH 60

Query: 61  NT--DSHIIELNSNKFDDSLESAIKLYQKWSHLNVSGILDQETLDQMFQPRCGVRDVFKF 120
           NT  DSHIIELNSNKFDDSLESAIKLYQKWSHLNVSGILDQETLDQMF+PRCGVRDVFKF
Sbjct: 61  NTSIDSHIIELNSNKFDDSLESAIKLYQKWSHLNVSGILDQETLDQMFKPRCGVRDVFKF 120

Query: 121 NSSKNLEDDLEMSSHYALFPGNLKWPDYKRHLTYVFTNNFPINFVPSVTEAMARWAAHSL 180
           NS+KNLEDDLEMSSHY LFPGNLKWPDYKRHLTYVFTNNFP++FVPSVTEAMARWAA SL
Sbjct: 121 NSNKNLEDDLEMSSHYTLFPGNLKWPDYKRHLTYVFTNNFPVDFVPSVTEAMARWAAQSL 180

Query: 181 FTFSEASDAQSADINISFQIKDHADGMPFDGPGGVVGHAFAPIDGRLHLDGDDSWSAGLE 240
           FTFSEASDAQSADINISFQIKDHADG+PFDGPGGVVGHAFAP DGRLHLDGDD+WSAG+E
Sbjct: 181 FTFSEASDAQSADINISFQIKDHADGLPFDGPGGVVGHAFAPTDGRLHLDGDDTWSAGME 240

Query: 241 EKKVNVMNAALHELGHVLGLAHSTLPQAVMWPYIESNALKNLNDDDIAGLHALYP 294
            +KVNVMN ALHELGHVLGLAHSTLPQAVMWPYI+SNALKNLNDDDIAGLHALYP
Sbjct: 241 VQKVNVMNVALHELGHVLGLAHSTLPQAVMWPYIDSNALKNLNDDDIAGLHALYP 295

[Close]
BLAST of Cucsa.158730 vs. NCBI nr
Match: gi|778708729|ref|XP_004142460.2| (PREDICTED: metalloendoproteinase 1-like [Cucumis sativus])

HSP 1 Score: 552.4 bits (1422), Expect = 4.9e-154
Identity = 267/295 (90.51%), Postives = 280/295 (94.92%), Query Frame = 1 

Query: 1   MTFKSLGLLFLVLVTIASHVTSKHNPILSPQRLYGCRKGDNVEGIHNIKKYLQRYGYLSH 60
           M FKSL LLFL+L +IASHV SKHN ILSP+RLYGCRK DNVEGIH+IKKYLQRYGYLSH
Sbjct: 1   MAFKSLQLLFLILASIASHVISKHNSILSPRRLYGCRKDDNVEGIHSIKKYLQRYGYLSH 60

Query: 61  NT--DSHIIELNSNKFDDSLESAIKLYQKWSHLNVSGILDQETLDQMFQPRCGVRDVFKF 120
           NT  DS+IIELNSNKFDDSLESAI+LYQKWSHLNVSGI+DQETLDQMFQPRCGV DVFKF
Sbjct: 61  NTSTDSNIIELNSNKFDDSLESAIRLYQKWSHLNVSGIIDQETLDQMFQPRCGVPDVFKF 120

Query: 121 NSSKNLEDDLEMSSHYALFPGNLKWPDYKRHLTYVFTNNFPINFVPSVTEAMARWAAHSL 180
           NSSKNLEDDLEMSSHYALFP N KWPDYKRHLTY+FTNNFPI+FVPS+TEAMARWAA SL
Sbjct: 121 NSSKNLEDDLEMSSHYALFPNNEKWPDYKRHLTYMFTNNFPIDFVPSMTEAMARWAAQSL 180

Query: 181 FTFSEASDAQSADINISFQIKDHADGMPFDGPGGVVGHAFAPIDGRLHLDGDDSWSAGLE 240
           FTFSEASDAQSADINISFQ+KDHADG  FDGPGGVVGHAFAP DGRLHLDGDD+WSAGLE
Sbjct: 181 FTFSEASDAQSADINISFQVKDHADGFAFDGPGGVVGHAFAPTDGRLHLDGDDTWSAGLE 240

Query: 241 EKKVNVMNAALHELGHVLGLAHSTLPQAVMWPYIESNALKNLNDDDIAGLHALYP 294
           EKKVNVMNAALHELGHVLGLAHSTLPQAVMWPYIESNALKNLNDDDIAG+HALYP
Sbjct: 241 EKKVNVMNAALHELGHVLGLAHSTLPQAVMWPYIESNALKNLNDDDIAGIHALYP 295

[Close]
BLAST of Cucsa.158730 vs. NCBI nr
Match: gi|778708752|ref|XP_011656276.1| (PREDICTED: metalloendoproteinase 1-like [Cucumis sativus])

HSP 1 Score: 538.5 bits (1386), Expect = 7.3e-150
Identity = 262/292 (89.73%), Postives = 273/292 (93.49%), Query Frame = 1 

Query: 4   KSLGLLFLVLVTIASHVTSKHNPILSPQRLYGCRKGDNVEGIHNIKKYLQRYGYLSHNT- 63
           KSL LLFLVL +IASHV S +N ILSP+RL GCRKGDNVEGIHNIKKYLQRYGYLSHNT 
Sbjct: 3   KSLQLLFLVLASIASHVISINNQILSPRRLNGCRKGDNVEGIHNIKKYLQRYGYLSHNTS 62

Query: 64  -DSHIIELNSNKFDDSLESAIKLYQKWSHLNVSGILDQETLDQMFQPRCGVRDVFKFNSS 123
            DS+IIELNSNKFDDSLESAI+LYQKWSHLNVSGILDQETLDQMFQ RCG+RDVFKFNSS
Sbjct: 63  TDSNIIELNSNKFDDSLESAIRLYQKWSHLNVSGILDQETLDQMFQQRCGIRDVFKFNSS 122

Query: 124 KNLEDDLEMSSHYALFPGNLKWPDYKRHLTYVFTNNFPINFVPSVTEAMARWAAHSLFTF 183
           KNLEDDLEMSSHY LFP N KWPDYKRHLTY+FTNNFP++FVPSVTEAMARWAA SLFTF
Sbjct: 123 KNLEDDLEMSSHYVLFPNNKKWPDYKRHLTYMFTNNFPVDFVPSVTEAMARWAAQSLFTF 182

Query: 184 SEASDAQSADINISFQIKDHADGMPFDGPGGVVGHAFAPIDGRLHLDGDDSWSAGLEEKK 243
           SEASDAQSADINISFQ+KDHADG  FDGPGGVVGHAFAP DGRLHLDGDDSWSAGLEE K
Sbjct: 183 SEASDAQSADINISFQVKDHADGSAFDGPGGVVGHAFAPTDGRLHLDGDDSWSAGLEENK 242

Query: 244 VNVMNAALHELGHVLGLAHSTLPQAVMWPYIESNALKNLNDDDIAGLHALYP 294
            NVMN ALHELGHVLGLAHSTLPQAVMWPYI+SNALKNLNDDDIAGLHALYP
Sbjct: 243 FNVMNVALHELGHVLGLAHSTLPQAVMWPYIDSNALKNLNDDDIAGLHALYP 294

[Close]
BLAST of Cucsa.158730 vs. NCBI nr
Match: gi|659118035|ref|XP_008458917.1| (PREDICTED: metalloendoproteinase 1-like [Cucumis melo])

HSP 1 Score: 478.0 bits (1229), Expect = 1.2e-131
Identity = 233/295 (78.98%), Postives = 254/295 (86.10%), Query Frame = 1 

Query: 1   MTFKSLGLLFLVLVTIASHVTSKHNPILSPQRLYGCRKGDNVEGIHNIKKYLQRYGYLSH 60
           M FKSL L FL+L +  SHV S+H P   PQ LYGCRKGDNVEGIH+IKKYL RYGYLSH
Sbjct: 1   MAFKSLQLFFLLLASFTSHVISRHIPTPIPQHLYGCRKGDNVEGIHSIKKYLHRYGYLSH 60

Query: 61  NTD-SHIIELNSNKFDDSLESAIKLYQKWSHLNVSGILDQETLDQMFQPRCGVRDVF-KF 120
           NT+ S  IELNSN FDD+LESA+KLYQKWS LNVSGILDQ+TLDQ+F+PRCG+ DV  K 
Sbjct: 61  NTNTSSHIELNSNTFDDTLESAVKLYQKWSKLNVSGILDQQTLDQIFKPRCGLPDVIMKS 120

Query: 121 NSSKNLEDDLEMSSHYALFPGNLKWPDYKRHLTYVFTNNFPINFVPSVTEAMARWAAHSL 180
           NS+KN EDDLE+SSHYALFPGN KWPDYKRHLTY FTNNFPI+FVPSVTEAM  WAAHSL
Sbjct: 121 NSNKNTEDDLEISSHYALFPGNQKWPDYKRHLTYTFTNNFPIDFVPSVTEAMKEWAAHSL 180

Query: 181 FTFSEASDAQSADINISFQIKDHADGMPFDGPGGVVGHAFAPIDGRLHLDGDDSWSAGLE 240
           FTFS+A+D Q  DINISFQ+ DH DG+PFDGPGGVVGHAFAP DGRLHLDGDD WSAGLE
Sbjct: 181 FTFSQAADDQVPDINISFQVGDHGDGVPFDGPGGVVGHAFAPTDGRLHLDGDDKWSAGLE 240

Query: 241 EKKVNVMNAALHELGHVLGLAHSTLPQAVMWPYIESNALKNLNDDDIAGLHALYP 294
            +KVNVMNAALHELGHVLGLAHST+ QAVMWPYI+  ALK L DDDIAGL ALYP
Sbjct: 241 TQKVNVMNAALHELGHVLGLAHSTVQQAVMWPYIDVYALKALTDDDIAGLTALYP 295

[Close]
BLAST of Cucsa.158730 vs. NCBI nr
Match: gi|659118025|ref|XP_008458911.1| (PREDICTED: metalloendoproteinase 1-like [Cucumis melo])

HSP 1 Score: 280.0 bits (715), Expect = 4.7e-72
Identity = 149/297 (50.17%), Postives = 189/297 (63.64%), Query Frame = 1 

Query: 9   LFLVLVTIASHVTS-----KHNP--ILSPQRLYGCRKGDNVEGIHNIKKYLQRYGYLSHN 68
           LFL+   I  H+TS      H P  +L P+ L G RK DN EGIH +K YLQRYGYLS  
Sbjct: 9   LFLLFAFITPHLTSARHLRNHKPSRLLFPEHLIGSRKNDNFEGIHKVKAYLQRYGYLSDE 68

Query: 69  TDSHIIELNSNKFDDSLESAIKLYQKWSHLNVSGILDQETLDQMFQPRCGVRDVFKFNSS 128
            +++   L+ + FDD LESAIK YQK   LNVSG+LD+ETL QM +PRCGV D F   + 
Sbjct: 69  KENN---LSIDAFDDDLESAIKSYQKVFKLNVSGVLDRETLQQMARPRCGVTDDFILVAQ 128

Query: 129 KNLEDDLEM----SSHYALFPGNLKWPDYKRHLTYVFTNNFPINFVPSVTEAMARWAAHS 188
           ++ E++        SHY  +P  LKWP  KRHLTY F +N+P     +V  A   W A++
Sbjct: 129 QDHENNNTNIQIGGSHYMFYPNKLKWPVEKRHLTYGFIHNYPPEHAAAVIRAFDTWEANT 188

Query: 189 LFTFSEASDAQSADINISFQIKDHADGMPFDGPGGVVGHAFAPIDGRLHLDGDDSWSAG- 248
            FTFS A+  Q+ADI +SF+  DH DG PFDGPGG +GHAF  IDGR+H DGD+ W  G 
Sbjct: 189 KFTFSHAARVQTADILLSFERGDHGDGKPFDGPGGALGHAFGAIDGRVHFDGDEQWETGS 248

Query: 249 LEEKKVNVMNAALHELGHVLGLAHSTLPQAVMWPYIESNALK-NLNDDDIAGLHALY 293
             ++  +V + ALHE+GH LGL HS  P AVMW  ++S   K +L  DDI G+HALY
Sbjct: 249 FWQEYFDVESVALHEIGHALGLGHSREPSAVMWANMDSGVTKTHLTIDDIEGIHALY 302

The following BLAST results are available for this feature:
BLAST of Cucsa.158730 vs. Swiss-Prot
Match NameE-valueIdentityDescription
2MMP_ARATH8.3e-4537.23Metalloendoproteinase 2-MMP OS=Arabidopsis thaliana GN=2MMP PE=1 SV=1[more]
5MMP_ARATH1.1e-4438.46Metalloendoproteinase 5-MMP OS=Arabidopsis thaliana GN=5MMP PE=1 SV=1[more]
MEP1_SOYBN4.6e-4339.16Metalloendoproteinase 1 OS=Glycine max PE=1 SV=2[more]
3MMP_ARATH6.0e-4335.05Metalloendoproteinase 3-MMP OS=Arabidopsis thaliana GN=3MMP PE=1 SV=1[more]
1MMP_ARATH6.6e-4237.36Metalloendoproteinase 1-MMP OS=Arabidopsis thaliana GN=1MMP PE=1 SV=1[more]
BLAST of Cucsa.158730 vs. TrEMBL
Match NameE-valueIdentityDescription
A0A0A0KNY8_CUCSA7.1e-16093.22Uncharacterized protein OS=Cucumis sativus GN=Csa_5G522910 PE=3 SV=1[more]
A0A0A0KX71_CUCSA3.4e-15490.51Uncharacterized protein OS=Cucumis sativus GN=Csa_5G631560 PE=3 SV=1[more]
A0A0A0KVD8_CUCSA5.1e-15089.73Uncharacterized protein OS=Cucumis sativus GN=Csa_5G633150 PE=3 SV=1[more]
A0A0A0KS35_CUCSA2.0e-6948.32Uncharacterized protein OS=Cucumis sativus GN=Csa_5G632070 PE=3 SV=1[more]
A0A0A0KU02_CUCSA9.8e-6948.12Uncharacterized protein OS=Cucumis sativus GN=Csa_5G632080 PE=3 SV=1[more]
BLAST of Cucsa.158730 vs. TAIR10
Match NameE-valueIdentityDescription
AT1G70170.14.7e-4637.23 matrix metalloproteinase[more]
AT1G59970.16.1e-4638.46 Matrixin family protein[more]
AT1G24140.13.4e-4435.05 Matrixin family protein[more]
AT4G16640.13.7e-4337.36 Matrixin family protein[more]
AT2G45040.15.5e-3937.31 Matrixin family protein[more]
BLAST of Cucsa.158730 vs. NCBI nr
Match NameE-valueIdentityDescription
gi|778708586|ref|XP_004142843.2|1.0e-15993.22PREDICTED: metalloendoproteinase 1-like [Cucumis sativus][more]
gi|778708729|ref|XP_004142460.2|4.9e-15490.51PREDICTED: metalloendoproteinase 1-like [Cucumis sativus][more]
gi|778708752|ref|XP_011656276.1|7.3e-15089.73PREDICTED: metalloendoproteinase 1-like [Cucumis sativus][more]
gi|659118035|ref|XP_008458917.1|1.2e-13178.98PREDICTED: metalloendoproteinase 1-like [Cucumis melo][more]
gi|659118025|ref|XP_008458911.1|4.7e-7250.17PREDICTED: metalloendoproteinase 1-like [Cucumis melo][more]
Annotated Terms
The following terms have been associated with this gene:
Vocabulary: INTERPRO
TermDefinition
IPR001818Pept_M10_metallopeptidase
IPR002477Peptidoglycan-bd-like
IPR006026Peptidase_Metallo
IPR021190Pept_M10A
IPR024079MetalloPept_cat_dom_sf
Vocabulary: Molecular Function
TermDefinition
GO:0004222metalloendopeptidase activity
GO:0008270zinc ion binding
GO:0008237metallopeptidase activity
Vocabulary: Biological Process
TermDefinition
GO:0006508proteolysis
Vocabulary: Cellular Component
TermDefinition
GO:0031012extracellular matrix
GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006508 proteolysis
cellular_component GO:0031012 extracellular matrix
molecular_function GO:0004222 metalloendopeptidase activity
molecular_function GO:0008270 zinc ion binding
molecular_function GO:0008237 metallopeptidase activity
Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Cucsa.158730.1Cucsa.158730.1mRNA


InterPro
Analysis Name: InterPro Annotations of cucumber (Gy14)
Date Performed: 2017-01-17
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001818Peptidase M10, metallopeptidasePFAMPF00413Peptidase_M10coord: 149..292
score: 3.0
IPR002477Peptidoglycan binding-likeGENE3DG3DSA:1.10.101.10coord: 44..106
score: 6.1
IPR002477Peptidoglycan binding-likePFAMPF01471PG_binding_1coord: 44..105
score: 4.4
IPR002477Peptidoglycan binding-likeunknownSSF47090PGBD-likecoord: 34..115
score: 5.02
IPR006026Peptidase, metallopeptidaseSMARTSM00235col_5coord: 139..293
score: 7.1
IPR021190Peptidase M10APRINTSPR00138MATRIXINcoord: 105..118
score: 4.5E-32coord: 247..272
score: 4.5E-32coord: 166..181
score: 4.5E-32coord: 280..293
score: 4.5E-32coord: 190..218
score: 4.5
IPR024079Metallopeptidase, catalytic domainGENE3DG3DSA:3.40.390.10coord: 107..293
score: 5.5
NoneNo IPR availablePANTHERPTHR10201MATRIX METALLOPROTEINASEcoord: 1..292
score: 1.1
NoneNo IPR availablePANTHERPTHR10201:SF152SUBFAMILY NOT NAMEDcoord: 1..292
score: 1.1
NoneNo IPR availableunknownSSF55486Metalloproteases ("zincins"), catalytic domaincoord: 136..293
score: 3.18

Synteny
The following gene(s) are orthologous to this gene:
GeneOrthologueOrganismBlock
Cucsa.158730MELO3C021497Melon (DHL92) v3.5.1cgymeB265
Cucsa.158730MELO3C021493.2Melon (DHL92) v3.6.1cgymedB265
The following gene(s) are paralogous to this gene:

None

The following block(s) are covering this gene:
GeneOrganismBlock
Cucsa.158730Cucurbita pepo (Zucchini)cgycpeB0424
Cucsa.158730Cucurbita pepo (Zucchini)cgycpeB0425
Cucsa.158730Bottle gourd (USVL1VR-Ls)cgylsiB251
Cucsa.158730Bottle gourd (USVL1VR-Ls)cgylsiB252
Cucsa.158730Melon (DHL92) v3.6.1cgymedB268
Cucsa.158730Silver-seed gourdcarcgyB0309
Cucsa.158730Silver-seed gourdcarcgyB0763
Cucsa.158730Cucumber (Chinese Long) v3cgycucB240
Cucsa.158730Cucumber (Chinese Long) v3cgycucB244
Cucsa.158730Watermelon (97103) v2cgywmbB270
Cucsa.158730Watermelon (97103) v2cgywmbB265
Cucsa.158730Wax gourdcgywgoB308
Cucsa.158730Wax gourdcgywgoB310
Cucsa.158730Cucumber (Gy14) v1cgycgyB036
Cucsa.158730Cucurbita maxima (Rimu)cgycmaB0439
Cucsa.158730Cucurbita maxima (Rimu)cgycmaB0441
Cucsa.158730Cucurbita moschata (Rifu)cgycmoB0437
Cucsa.158730Cucurbita moschata (Rifu)cgycmoB0439
Cucsa.158730Cucurbita moschata (Rifu)cgycmoB0442
Cucsa.158730Cucumber (Chinese Long) v2cgycuB226
Cucsa.158730Wild cucumber (PI 183967)cgycpiB232
Cucsa.158730Wild cucumber (PI 183967)cgycpiB236
Cucsa.158730Cucumber (Chinese Long) v2cgycuB230
Cucsa.158730Melon (DHL92) v3.5.1cgymeB268
Cucsa.158730Watermelon (Charleston Gray)cgywcgB264
Cucsa.158730Watermelon (Charleston Gray)cgywcgB265
Cucsa.158730Watermelon (Charleston Gray)cgywcgB268
Cucsa.158730Watermelon (97103) v1cgywmB280
Cucsa.158730Watermelon (97103) v1cgywmB283