Cucsa.155520 (gene) Cucumber (Gy14) v1

NameCucsa.155520
Typegene
OrganismCucumis sativus (Cucumber (Gy14) v1)
DescriptionPhenylalanine ammonia-lyase
Locationscaffold01136 : 265076 .. 267105 (-)
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDS
Hold the cursor over a type above to highlight its positions in the sequence below.
CTGCCAATGCTCTCAGCCAGACCCATTTTGAAGAGGTTTGTCGCATGTTATCAGAGTTTCAAGAGGCTCAAACTGTTGATGTTGAAGGCACCACGCTTACTGTCGCTCAGGTAGCCACCATTGCTCGTCGAAGTGATGTCAAAGTCATCTTGAATGAATCTGTGGCACATGATCGTGTGGCCGAAAGTGCAAAATGGGTTGCTGAGAAAGTGTCGAGCGGAATAGATATATATGGAGTTACCACGGGCTTTGGTGCCACCTCCCATCTCCGTACGACCAAAATATCCGACCTCCAAACAGAGCTTATTAGATTCCTCAATGCTGGTGTCATTGGAAAGGATAATCTTCCGTCTTGCTACTCGAAGGTGGCGATGCTTGTTAGAACAAACACATTGATGCAAGGCTACTCAGGGATTCGTTGGGAAATTCTGGAAGCTCTAGTGAAGTTGATGAATGAGAATTTGATCCCTAAGTTGCCTTTGAGAGGGACGATTACTGCTTCTGGAGATCTTGTTCCTCTGTCTTATATTGCTGGCGTTGTCACTGGAAGGCATAATTCCAAGGTTACTACCATTGAAGGTGATGAGATTACTGCCATTGAAGCTCTCAAACGAGCTGGAGTTGCAGGCCCATTTGAGCTTCAAGCAAAGGAAGGTTTAGCTTTAGTCAATGGAACTGCTGTTGGGTCAGCTGTTGCTGCTACAGTTTGCTACGACGCCAACATTCTGGCTCTTTTAGCCAGTGTTCTCTCTGCTTTGTTTTGTGAAGCCATGCTTGGGAAGCCAGAATTTACAGACCCGTTAACCCATAAGCTTAAGCACCACCCCGGTCAGATTGAATCCGCTGCAGTCATGGAGTTCCTCTTGAACCAAAGCGATTTCATGAAAGAAGCAAAAATCTGCAATGAAAATGACCCATTGACAAAGCCAAAACAAGATCGTTATGCACTTCGAACCTCTCCACAATGGCTCGGACCCCAAATCGAAGTCATTCGAATGGCAACTCATTCGATCGAAAGGGAAATTAACTCTGTGAATGATAACCCATTAATTGATGTTAGTCGAGATGTAGCTGTACATGGAGGAAACTTTCAGGGGACCCCAATTGGAGTTTCCATGGATAATCTTCGAATCGCCATCGCTGCAATTGGAAAGCTAATGTTTGCCCAATTCTCTGAACTCGTATGTCATCATTACAATAATGGATTACCATCTAATTTGAGCGGCGGACCAAATCCTAGCTTGGACTACGGCTTCAAAGGGGCGGAAATCGCCATGGCTGCTTATTGCTCTGAGCTCCAATACTTAGCAAATCCTGTCACTACCCATGTCCAAAGTGCAGAACAACACAATCAAGATGTAAACTCTCTAGGTTTAATCTCAGCCAGAAAATCCGCTGAAGCCATTGAAATACTAAAGCTCATGTCTGCTACATACATGGTAGCGCTTTGCCAAGCAATCGATTTAAGACAATTCGAAGAAAATATGAGGGAGGTAGTGAAAAATGCTCTTCTACAAACCATCAGAAAAACACTCTACATGGCAGAGTATGGATCACTTCTCGAATCACGATTTTGTGAGAAAGATCTCTTGCAAGTAATCGAGCGCGAGGAAGTCTTCTCCTACATCGACGATCCCACCAACACATCCTACACCCTAATCCCAAAATTACTAGAAGTTCTAGTTGAAAAAGCATTGAAAGGAGTTGCAACAGGCAAAACAGATAACGGAAACGAAACAGGGATTGCCATATTCAAGCAAATTCCAGAGTTTCTCGAGGAATTAAAGGCGAAATTGGAAAAAGACGTTGAAAATGCAAGAGGGAGATTCGAGAATGGTGATTTCGAAATTGAAAATAGAATCAAGAAATGCAGCACTTACCCAATCTACCGATTCGTGAGAAATGAAGTGGGGACTCAACTGTTGAGTGGAGCAAAGAAGGTTAGCCCTGGAGAAGATATTGAGAAAGTGGTGGAGGCCATTGATGAAGGGAAGCTTGGAAATATTTTGATGGAGTGTTTGAAGAATTAG

mRNA sequence

CTGCCAATGCTCTCAGCCAGACCCATTTTGAAGAGGTTTGTCGCATGTTATCAGAGTTTCAAGAGGCTCAAACTGTTGATGTTGAAGGCACCACGCTTACTGTCGCTCAGGTAGCCACCATTGCTCGTCGAAGTGATGTCAAAGTCATCTTGAATGAATCTGTGGCACATGATCGTGTGGCCGAAAGTGCAAAATGGGTTGCTGAGAAAGTGTCGAGCGGAATAGATATATATGGAGTTACCACGGGCTTTGGTGCCACCTCCCATCTCCGTACGACCAAAATATCCGACCTCCAAACAGAGCTTATTAGATTCCTCAATGCTGGTGTCATTGGAAAGGATAATCTTCCGTCTTGCTACTCGAAGGTGGCGATGCTTGTTAGAACAAACACATTGATGCAAGGCTACTCAGGGATTCGTTGGGAAATTCTGGAAGCTCTAGTGAAGTTGATGAATGAGAATTTGATCCCTAAGTTGCCTTTGAGAGGGACGATTACTGCTTCTGGAGATCTTGTTCCTCTGTCTTATATTGCTGGCGTTGTCACTGGAAGGCATAATTCCAAGGTTACTACCATTGAAGGTGATGAGATTACTGCCATTGAAGCTCTCAAACGAGCTGGAGTTGCAGGCCCATTTGAGCTTCAAGCAAAGGAAGGTTTAGCTTTAGTCAATGGAACTGCTGTTGGGTCAGCTGTTGCTGCTACAGTTTGCTACGACGCCAACATTCTGGCTCTTTTAGCCAGTGTTCTCTCTGCTTTGTTTTGTGAAGCCATGCTTGGGAAGCCAGAATTTACAGACCCGTTAACCCATAAGCTTAAGCACCACCCCGGTCAGATTGAATCCGCTGCAGTCATGGAGTTCCTCTTGAACCAAAGCGATTTCATGAAAGAAGCAAAAATCTGCAATGAAAATGACCCATTGACAAAGCCAAAACAAGATCGTTATGCACTTCGAACCTCTCCACAATGGCTCGGACCCCAAATCGAAGTCATTCGAATGGCAACTCATTCGATCGAAAGGGAAATTAACTCTGTGAATGATAACCCATTAATTGATGTTAGTCGAGATGTAGCTGTACATGGAGGAAACTTTCAGGGGACCCCAATTGGAGTTTCCATGGATAATCTTCGAATCGCCATCGCTGCAATTGGAAAGCTAATGTTTGCCCAATTCTCTGAACTCGTATGTCATCATTACAATAATGGATTACCATCTAATTTGAGCGGCGGACCAAATCCTAGCTTGGACTACGGCTTCAAAGGGGCGGAAATCGCCATGGCTGCTTATTGCTCTGAGCTCCAATACTTAGCAAATCCTGTCACTACCCATGTCCAAAGTGCAGAACAACACAATCAAGATGTAAACTCTCTAGGTTTAATCTCAGCCAGAAAATCCGCTGAAGCCATTGAAATACTAAAGCTCATGTCTGCTACATACATGGTAGCGCTTTGCCAAGCAATCGATTTAAGACAATTCGAAGAAAATATGAGGGAGGTAGTGAAAAATGCTCTTCTACAAACCATCAGAAAAACACTCTACATGGCAGAGTATGGATCACTTCTCGAATCACGATTTTGTGAGAAAGATCTCTTGCAAGTAATCGAGCGCGAGGAAGTCTTCTCCTACATCGACGATCCCACCAACACATCCTACACCCTAATCCCAAAATTACTAGAAGTTCTAGTTGAAAAAGCATTGAAAGGAGTTGCAACAGGCAAAACAGATAACGGAAACGAAACAGGGATTGCCATATTCAAGCAAATTCCAGAGTTTCTCGAGGAATTAAAGGCGAAATTGGAAAAAGACGTTGAAAATGCAAGAGGGAGATTCGAGAATGGTGATTTCGAAATTGAAAATAGAATCAAGAAATGCAGCACTTACCCAATCTACCGATTCGTGAGAAATGAAGTGGGGACTCAACTGTTGAGTGGAGCAAAGAAGGTTAGCCCTGGAGAAGATATTGAGAAAGTGGTGGAGGCCATTGATGAAGGGAAGCTTGGAAATATTTTGATGGAGTGTTTGAAGAATTAG

Coding sequence (CDS)

CTGCCAATGCTCTCAGCCAGACCCATTTTGAAGAGGTTTGTCGCATGTTATCAGAGTTTCAAGAGGCTCAAACTGTTGATGTTGAAGGCACCACGCTTACTGTCGCTCAGGTAGCCACCATTGCTCGTCGAAGTGATGTCAAAGTCATCTTGAATGAATCTGTGGCACATGATCGTGTGGCCGAAAGTGCAAAATGGGTTGCTGAGAAAGTGTCGAGCGGAATAGATATATATGGAGTTACCACGGGCTTTGGTGCCACCTCCCATCTCCGTACGACCAAAATATCCGACCTCCAAACAGAGCTTATTAGATTCCTCAATGCTGGTGTCATTGGAAAGGATAATCTTCCGTCTTGCTACTCGAAGGTGGCGATGCTTGTTAGAACAAACACATTGATGCAAGGCTACTCAGGGATTCGTTGGGAAATTCTGGAAGCTCTAGTGAAGTTGATGAATGAGAATTTGATCCCTAAGTTGCCTTTGAGAGGGACGATTACTGCTTCTGGAGATCTTGTTCCTCTGTCTTATATTGCTGGCGTTGTCACTGGAAGGCATAATTCCAAGGTTACTACCATTGAAGGTGATGAGATTACTGCCATTGAAGCTCTCAAACGAGCTGGAGTTGCAGGCCCATTTGAGCTTCAAGCAAAGGAAGGTTTAGCTTTAGTCAATGGAACTGCTGTTGGGTCAGCTGTTGCTGCTACAGTTTGCTACGACGCCAACATTCTGGCTCTTTTAGCCAGTGTTCTCTCTGCTTTGTTTTGTGAAGCCATGCTTGGGAAGCCAGAATTTACAGACCCGTTAACCCATAAGCTTAAGCACCACCCCGGTCAGATTGAATCCGCTGCAGTCATGGAGTTCCTCTTGAACCAAAGCGATTTCATGAAAGAAGCAAAAATCTGCAATGAAAATGACCCATTGACAAAGCCAAAACAAGATCGTTATGCACTTCGAACCTCTCCACAATGGCTCGGACCCCAAATCGAAGTCATTCGAATGGCAACTCATTCGATCGAAAGGGAAATTAACTCTGTGAATGATAACCCATTAATTGATGTTAGTCGAGATGTAGCTGTACATGGAGGAAACTTTCAGGGGACCCCAATTGGAGTTTCCATGGATAATCTTCGAATCGCCATCGCTGCAATTGGAAAGCTAATGTTTGCCCAATTCTCTGAACTCGTATGTCATCATTACAATAATGGATTACCATCTAATTTGAGCGGCGGACCAAATCCTAGCTTGGACTACGGCTTCAAAGGGGCGGAAATCGCCATGGCTGCTTATTGCTCTGAGCTCCAATACTTAGCAAATCCTGTCACTACCCATGTCCAAAGTGCAGAACAACACAATCAAGATGTAAACTCTCTAGGTTTAATCTCAGCCAGAAAATCCGCTGAAGCCATTGAAATACTAAAGCTCATGTCTGCTACATACATGGTAGCGCTTTGCCAAGCAATCGATTTAAGACAATTCGAAGAAAATATGAGGGAGGTAGTGAAAAATGCTCTTCTACAAACCATCAGAAAAACACTCTACATGGCAGAGTATGGATCACTTCTCGAATCACGATTTTGTGAGAAAGATCTCTTGCAAGTAATCGAGCGCGAGGAAGTCTTCTCCTACATCGACGATCCCACCAACACATCCTACACCCTAATCCCAAAATTACTAGAAGTTCTAGTTGAAAAAGCATTGAAAGGAGTTGCAACAGGCAAAACAGATAACGGAAACGAAACAGGGATTGCCATATTCAAGCAAATTCCAGAGTTTCTCGAGGAATTAAAGGCGAAATTGGAAAAAGACGTTGAAAATGCAAGAGGGAGATTCGAGAATGGTGATTTCGAAATTGAAAATAGAATCAAGAAATGCAGCACTTACCCAATCTACCGATTCGTGAGAAATGAAGTGGGGACTCAACTGTTGAGTGGAGCAAAGAAGGTTAGCCCTGGAGAAGATATTGAGAAAGTGGTGGAGGCCATTGATGAAGGGAAGCTTGGAAATATTTTGATGGAGTGTTTGAAGAATTAG

Protein sequence

ANALSQTHFEEVCRMLSEFQEAQTVDVEGTTLTVAQVATIARRSDVKVILNESVAHDRVAESAKWVAEKVSSGIDIYGVTTGFGATSHLRTTKISDLQTELIRFLNAGVIGKDNLPSCYSKVAMLVRTNTLMQGYSGIRWEILEALVKLMNENLIPKLPLRGTITASGDLVPLSYIAGVVTGRHNSKVTTIEGDEITAIEALKRAGVAGPFELQAKEGLALVNGTAVGSAVAATVCYDANILALLASVLSALFCEAMLGKPEFTDPLTHKLKHHPGQIESAAVMEFLLNQSDFMKEAKICNENDPLTKPKQDRYALRTSPQWLGPQIEVIRMATHSIEREINSVNDNPLIDVSRDVAVHGGNFQGTPIGVSMDNLRIAIAAIGKLMFAQFSELVCHHYNNGLPSNLSGGPNPSLDYGFKGAEIAMAAYCSELQYLANPVTTHVQSAEQHNQDVNSLGLISARKSAEAIEILKLMSATYMVALCQAIDLRQFEENMREVVKNALLQTIRKTLYMAEYGSLLESRFCEKDLLQVIEREEVFSYIDDPTNTSYTLIPKLLEVLVEKALKGVATGKTDNGNETGIAIFKQIPEFLEELKAKLEKDVENARGRFENGDFEIENRIKKCSTYPIYRFVRNEVGTQLLSGAKKVSPGEDIEKVVEAIDEGKLGNILMECLKN*
BLAST of Cucsa.155520 vs. Swiss-Prot
Match: PALY_PINTA (Phenylalanine ammonia-lyase OS=Pinus taeda GN=PAL PE=3 SV=1)

HSP 1 Score: 844.3 bits (2180), Expect = 9.0e-244
Identity = 430/676 (63.61%), Postives = 527/676 (77.96%), Query Frame = 1

Query: 1   ANALSQTHFEEVCRMLSEFQEAQTVDVEGTTLTVAQVATIARRSDVKVILNESVAHDRVA 60
           A A+  +HFEEV  M+  +  A+ + +EG +LT++ VA +ARRS VKV L+ + A  RV 
Sbjct: 38  AKAMEGSHFEEVKAMVDSYFGAKEISIEGKSLTISDVAAVARRSQVKVKLDAAAAKSRVE 97

Query: 61  ESAKWVAEKVSSGIDIYGVTTGFGATSHLRTTKISDLQTELIRFLNAGVIGK---DNLPS 120
           ES+ WV  +++ G D YGVTTGFGATSH RT + ++LQ ELIRFLNAGV+GK   + L  
Sbjct: 98  ESSNWVLTQMTKGTDTYGVTTGFGATSHRRTNQGAELQKELIRFLNAGVLGKCPENVLSE 157

Query: 121 CYSKVAMLVRTNTLMQGYSGIRWEILEALVKLMNENLIPKLPLRGTITASGDLVPLSYIA 180
             ++ AMLVRTNTL+QGYSG+RW+ILE + KL+N  L PKLPLRGTITASGDLVPLSYIA
Sbjct: 158 DTTRAAMLVRTNTLLQGYSGVRWDILETVEKLLNAWLTPKLPLRGTITASGDLVPLSYIA 217

Query: 181 GVVTGRHNSKVTTIEGDEITAIEALKRAGVAGPFELQAKEGLALVNGTAVGSAVAATVCY 240
           G++TGR NS+V + +G E++  EALK+ G+  PFELQ KEGLA+VNGT+VG+A+A+ VC+
Sbjct: 218 GLLTGRPNSRVRSRDGIEMSGAEALKKVGLEKPFELQPKEGLAIVNGTSVGAALASIVCF 277

Query: 241 DANILALLASVLSALFCEAMLGKPEFTDPLTHKLKHHPGQIESAAVMEFLLNQSDFMKEA 300
           DAN+LALL+ V+SA+FCE M GKPEFTDPLTHKLKHHPGQ+E+AA+ME++L+ S +MK A
Sbjct: 278 DANVLALLSEVISAMFCEVMNGKPEFTDPLTHKLKHHPGQMEAAAIMEYVLDGSSYMKHA 337

Query: 301 KICNENDPLTKPKQDRYALRTSPQWLGPQIEVIRMATHSIEREINSVNDNPLIDVSRDVA 360
              +E +PL KPKQDRY LRTSPQWLGPQ+E+IR ATH IEREINSVNDNP+IDV+RD A
Sbjct: 338 AKLHEMNPLQKPKQDRYGLRTSPQWLGPQVEIIRSATHMIEREINSVNDNPVIDVARDKA 397

Query: 361 VHGGNFQGTPIGVSMDNLRIAIAAIGKLMFAQFSELVCHHYNNGLPSNLSGGPNPSLDYG 420
           +HGGNFQGTPIGVSMDNLR++I+AIGKLMFAQFSELV  +YN GLPSNLSGGPNPSLDYG
Sbjct: 398 LHGGNFQGTPIGVSMDNLRLSISAIGKLMFAQFSELVNDYYNGGLPSNLSGGPNPSLDYG 457

Query: 421 FKGAEIAMAAYCSELQYLANPVTTHVQSAEQHNQDVNSLGLISARKSAEAIEILKLMSAT 480
            KGAEIAMA+Y SEL YLANPVT+HVQSAEQHNQDVNSLGL+SARKSAEAI+ILKLM +T
Sbjct: 458 LKGAEIAMASYTSELLYLANPVTSHVQSAEQHNQDVNSLGLVSARKSAEAIDILKLMLST 517

Query: 481 YMVALCQAIDLRQFEENMREVVKNALLQTIRKTLYMAEYGSLLESRFCEKDLLQVIEREE 540
           Y+ ALCQA+DLR  EENM   VK  + Q  +KTL     G LL  RFCEKDLLQV++ E 
Sbjct: 518 YLTALCQAVDLRHLEENMLATVKQIVSQVAKKTLSTGLNGELLPGRFCEKDLLQVVDNEH 577

Query: 541 VFSYIDDPTNTSYTLIPKLLEVLVEKALKGVATGKTDNGNETGIAIFKQIPEFLEELKAK 600
           VFSYIDDP N SY L  KL  +LVE A K     K  N      +IF +IP F  ELKA+
Sbjct: 578 VFSYIDDPCNASYPLTQKLRNILVEHAFKNAEGEKDPN-----TSIFNKIPVFEAELKAQ 637

Query: 601 LEKDVENARGRFENGDFEIENRIKKCSTYPIYRFVRNEVGTQLLSGAKKVSPGEDIEKVV 660
           LE  V  AR  ++ G   + +RI++C +YP+Y FVRN++GT+LLSG + +SPGE IE V 
Sbjct: 638 LEPQVSLARESYDKGTSPLPDRIQECRSYPLYEFVRNQLGTKLLSGTRTISPGEVIEVVY 697

Query: 661 EAIDEGKLGNILMECL 674
           +AI E K+   L +CL
Sbjct: 698 DAISEDKVIVPLFKCL 708

BLAST of Cucsa.155520 vs. Swiss-Prot
Match: PALY_CAMSI (Phenylalanine ammonia-lyase OS=Camellia sinensis GN=PAL PE=2 SV=1)

HSP 1 Score: 815.8 bits (2106), Expect = 3.4e-235
Identity = 433/681 (63.58%), Postives = 530/681 (77.83%), Query Frame = 1

Query: 1   ANALSQTHFEEVCRMLSEFQEAQTVDVEGTTLTVAQVATIA-RRSDVKVILNESVAHDRV 60
           A A+  +H EEV  M+ EF++   V + G TLT++QVA IA R S+V V L+ES A + V
Sbjct: 31  AEAMKGSHLEEVKGMVEEFRKP-VVRLGGETLTISQVAAIAVRGSEVAVELSES-AREGV 90

Query: 61  AESAKWVAEKVSSGIDIYGVTTGFGATSHLRTTKISDLQTELIRFLNAGVIGKDN----- 120
             S+ WV E ++ G D YGVTTGFGATSH RT +   LQ ELIRFLNAG+ G        
Sbjct: 91  KASSDWVMESMNKGTDSYGVTTGFGATSHRRTKEGGALQKELIRFLNAGIFGNGTESCHT 150

Query: 121 LPSCYSKVAMLVRTNTLMQGYSGIRWEILEALVKLMNENLIPKLPLRGTITASGDLVPLS 180
           LP   ++ AMLVR NTL+QGYSGIR+EILEA+ K +N N+ P LPLRGTITASGDLVPLS
Sbjct: 151 LPQSATRAAMLVRINTLLQGYSGIRFEILEAISKFLNNNITPCLPLRGTITASGDLVPLS 210

Query: 181 YIAGVVTGRHNSKVTTIEGDEITAIEALKRAGVAGPF-ELQAKEGLALVNGTAVGSAVAA 240
           YIAG++TGRHNSK     G+ +   EA + AGV G F ELQ KEGLALVNGTAVGS +A+
Sbjct: 211 YIAGLLTGRHNSKAVGPTGEILHPKEAFRLAGVEGGFFELQPKEGLALVNGTAVGSGLAS 270

Query: 241 TVCYDANILALLASVLSALFCEAMLGKPEFTDPLTHKLKHHPGQIESAAVMEFLLNQSDF 300
            V ++ANILA+L+ VLSA+F E M GKPEFTD LTHKLKHHPGQIE+AA+ME +L+ S +
Sbjct: 271 MVLFEANILAVLSEVLSAIFAEVMQGKPEFTDHLTHKLKHHPGQIEAAAIMEHILDGSSY 330

Query: 301 MKEAKICNENDPLTKPKQDRYALRTSPQWLGPQIEVIRMATHSIEREINSVNDNPLIDVS 360
           +K A+  +E DPL KPKQDRYALRTSPQWLGP IEVIR +T SIEREINSVNDNPLI+VS
Sbjct: 331 VKAAQKLHEMDPLQKPKQDRYALRTSPQWLGPLIEVIRSSTKSIEREINSVNDNPLINVS 390

Query: 361 RDVAVHGGNFQGTPIGVSMDNLRIAIAAIGKLMFAQFSELVCHHYNNGLPSNLSGGPNPS 420
           R+ A+HGGNFQGTPIGVSMDN R+A+A+IGKLMFAQFSELV   YNNGLPSNLSGG NPS
Sbjct: 391 RNKALHGGNFQGTPIGVSMDNTRLAVASIGKLMFAQFSELVNDFYNNGLPSNLSGGRNPS 450

Query: 421 LDYGFKGAEIAMAAYCSELQYLANPVTTHVQSAEQHNQDVNSLGLISARKSAEAIEILKL 480
           LDYGFKGAEIAMAAYCSELQ+LANPVT HVQSAEQHNQDVNSLGLIS+RK+AEA++ILKL
Sbjct: 451 LDYGFKGAEIAMAAYCSELQFLANPVTNHVQSAEQHNQDVNSLGLISSRKTAEAVDILKL 510

Query: 481 MSATYMVALCQAIDLRQFEENMREVVKNALLQTIRKTLYMAEYGSLLESRFCEKDLLQVI 540
           MS+TY+VALCQA+DLR FEEN+R  VK+ + Q  ++ L M   G L  SRFCEKDLL+V+
Sbjct: 511 MSSTYLVALCQAVDLRHFEENLRNTVKSTVSQVAKRVLTMGVNGELHPSRFCEKDLLRVV 570

Query: 541 EREEVFSYIDDPTNTSYTLIPKLLEVLVEKALKGVATGKTDNGNETGIAIFKQIPEFLEE 600
           +RE +F+YIDDP + +Y L+ KL +VLVE ALK    G+++    T  +IF++I  F EE
Sbjct: 571 DREYIFAYIDDPCSATYPLMQKLRQVLVEHALK---NGESEKNLST--SIFQKIRAFEEE 630

Query: 601 LKAKLEKDVENARGRFENGDFEIENRIKKCSTYPIYRFVRNEVGTQLLSGAKKVSPGEDI 660
           +K  L K+VE+ R   ENG+  I NRIK+C +YP+Y+FVR E+GT+LL+G K  SPGE+ 
Sbjct: 631 IKTLLPKEVESTRAAIENGNSAIPNRIKECRSYPLYKFVREELGTELLTGEKVRSPGEEF 690

Query: 661 EKVVEAIDEGKLGNILMECLK 675
           +KV  A+ +G++ + LM+CLK
Sbjct: 691 DKVFTALCKGEMIDPLMDCLK 704

BLAST of Cucsa.155520 vs. Swiss-Prot
Match: PAL2_ARATH (Phenylalanine ammonia-lyase 2 OS=Arabidopsis thaliana GN=PAL2 PE=1 SV=2)

HSP 1 Score: 814.7 bits (2103), Expect = 7.6e-235
Identity = 428/681 (62.85%), Postives = 530/681 (77.83%), Query Frame = 1

Query: 1   ANALSQTHFEEVCRMLSEFQEAQTVDVEGTTLTVAQVATIAR-RSDVKVILNESVAHDRV 60
           A+ +  +H +EV +M+ E++    V++ G TLT+ QVA I+     VKV L E+ +   V
Sbjct: 34  ADQMKGSHLDEVKKMVEEYRRP-VVNLGGETLTIGQVAAISTVGGSVKVELAET-SRAGV 93

Query: 61  AESAKWVAEKVSSGIDIYGVTTGFGATSHLRTTKISDLQTELIRFLNAGVIGKDN----- 120
             S+ WV E ++ G D YGVTTGFGATSH RT   + LQTELIRFLNAG+ G        
Sbjct: 94  KASSDWVMESMNKGTDSYGVTTGFGATSHRRTKNGTALQTELIRFLNAGIFGNTKETCHT 153

Query: 121 LPSCYSKVAMLVRTNTLMQGYSGIRWEILEALVKLMNENLIPKLPLRGTITASGDLVPLS 180
           LP   ++ AMLVR NTL+QGYSGIR+EILEA+  L+N N+ P LPLRGTITASGDLVPLS
Sbjct: 154 LPQSATRAAMLVRVNTLLQGYSGIRFEILEAITSLLNHNISPSLPLRGTITASGDLVPLS 213

Query: 181 YIAGVVTGRHNSKVTTIEGDEITAIEALKRAGVA-GPFELQAKEGLALVNGTAVGSAVAA 240
           YIAG++TGR NSK T  +G+ +TA EA ++AG++ G F+LQ KEGLALVNGTAVGS +A+
Sbjct: 214 YIAGLLTGRPNSKATGPDGESLTAKEAFEKAGISTGFFDLQPKEGLALVNGTAVGSGMAS 273

Query: 241 TVCYDANILALLASVLSALFCEAMLGKPEFTDPLTHKLKHHPGQIESAAVMEFLLNQSDF 300
            V ++AN+ A+LA VLSA+F E M GKPEFTD LTH+LKHHPGQIE+AA+ME +L+ S +
Sbjct: 274 MVLFEANVQAVLAEVLSAIFAEVMSGKPEFTDHLTHRLKHHPGQIEAAAIMEHILDGSSY 333

Query: 301 MKEAKICNENDPLTKPKQDRYALRTSPQWLGPQIEVIRMATHSIEREINSVNDNPLIDVS 360
           MK A+  +E DPL KPKQDRYALRTSPQWLGPQIEVIR AT SIEREINSVNDNPLIDVS
Sbjct: 334 MKLAQKVHEMDPLQKPKQDRYALRTSPQWLGPQIEVIRQATKSIEREINSVNDNPLIDVS 393

Query: 361 RDVAVHGGNFQGTPIGVSMDNLRIAIAAIGKLMFAQFSELVCHHYNNGLPSNLSGGPNPS 420
           R+ A+HGGNFQGTPIGVSMDN R+AIAAIGKLMFAQFSELV   YNNGLPSNL+   NPS
Sbjct: 394 RNKAIHGGNFQGTPIGVSMDNTRLAIAAIGKLMFAQFSELVNDFYNNGLPSNLTASSNPS 453

Query: 421 LDYGFKGAEIAMAAYCSELQYLANPVTTHVQSAEQHNQDVNSLGLISARKSAEAIEILKL 480
           LDYGFKGAEIAMA+YCSELQYLANPVT+HVQSAEQHNQDVNSLGLIS+RK++EA++ILKL
Sbjct: 454 LDYGFKGAEIAMASYCSELQYLANPVTSHVQSAEQHNQDVNSLGLISSRKTSEAVDILKL 513

Query: 481 MSATYMVALCQAIDLRQFEENMREVVKNALLQTIRKTLYMAEYGSLLESRFCEKDLLQVI 540
           MS T++V +CQA+DLR  EEN+R+ VKN + Q  +K L     G L  SRFCEKDLL+V+
Sbjct: 514 MSTTFLVGICQAVDLRHLEENLRQTVKNTVSQVAKKVLTTGINGELHPSRFCEKDLLKVV 573

Query: 541 EREEVFSYIDDPTNTSYTLIPKLLEVLVEKALKGVATGKTDNGNETGIAIFKQIPEFLEE 600
           +RE+VF+Y+DDP + +Y L+ +L +V+V+ AL   + G+T+    T  +IF++I  F EE
Sbjct: 574 DREQVFTYVDDPCSATYPLMQRLRQVIVDHAL---SNGETEKNAVT--SIFQKIGAFEEE 633

Query: 601 LKAKLEKDVENARGRFENGDFEIENRIKKCSTYPIYRFVRNEVGTQLLSGAKKVSPGEDI 660
           LKA L K+VE AR  + NG   I NRIK+C +YP+YRFVR E+GT+LL+G K VSPGE+ 
Sbjct: 634 LKAVLPKEVEAARAAYGNGTAPIPNRIKECRSYPLYRFVREELGTKLLTGEKVVSPGEEF 693

Query: 661 EKVVEAIDEGKLGNILMECLK 675
           +KV  A+ EGKL + LM+CLK
Sbjct: 694 DKVFTAMCEGKLIDPLMDCLK 707

BLAST of Cucsa.155520 vs. Swiss-Prot
Match: PAL1_DAUCA (Phenylalanine ammonia-lyase 1 OS=Daucus carota GN=PAL1 PE=3 SV=1)

HSP 1 Score: 813.9 bits (2101), Expect = 1.3e-234
Identity = 434/681 (63.73%), Postives = 527/681 (77.39%), Query Frame = 1

Query: 1   ANALSQTHFEEVCRMLSEFQEAQTVDVEGTTLTVAQVATIARRSDVKVILNESVAHDRVA 60
           A AL+ +H +EV RM++EF++   V + G TLTV+QVA IA  S VKV L ES A   V 
Sbjct: 27  AEALTGSHLDEVKRMVAEFRKPM-VQLGGETLTVSQVAAIAAGS-VKVELAES-ARAGVK 86

Query: 61  ESAKWVAEKVSSGIDIYGVTTGFGATSHLRTTKISDLQTELIRFLNAGVIGKDN-----L 120
            S+ WV E ++ G D YGVTTGFGATSH RT +   LQ ELIRFLNAG+ G  N     L
Sbjct: 87  ASSDWVMESMNKGTDSYGVTTGFGATSHRRTKQGGALQKELIRFLNAGIFGSGNDSSNIL 146

Query: 121 PSCYSKVAMLVRTNTLMQGYSGIRWEILEALVKLMNENLIPKLPLRGTITASGDLVPLSY 180
           P   ++ AMLVR NTL+QGYSGIR+EILEA+ K +N+N+ P LPLRGTITASGDLVPLSY
Sbjct: 147 PHSATRAAMLVRINTLLQGYSGIRFEILEAITKFLNQNITPCLPLRGTITASGDLVPLSY 206

Query: 181 IAGVVTGRHNSKVTTIEGDEITAIEALKRAGVAGPF-ELQAKEGLALVNGTAVGSAVAAT 240
           IAG++TGR NSK     G+ +TA EA K AGV G F ELQ KEGLALVNGTAVGS +A+ 
Sbjct: 207 IAGLLTGRPNSKAVGPTGENLTAAEAFKLAGVDGGFFELQPKEGLALVNGTAVGSGMASM 266

Query: 241 VCYDANILALLASVLSALFCEAMLGKPEFTDPLTHKLKHHPGQIESAAVMEFLLNQSDFM 300
           V ++ NILA+LA V+SA+F E M GKPEFTD LTHKLKHHPGQIE+AA+ME +L+ S ++
Sbjct: 267 VLFETNILAVLAEVMSAIFAEVMQGKPEFTDHLTHKLKHHPGQIEAAAIMEHILDGSSYV 326

Query: 301 KEAKICNENDPLTKPKQDRYALRTSPQWLGPQIEVIRMATHSIEREINSVNDNPLIDVSR 360
           K A+  +E DPL KPKQDRYALRTSPQWLGPQIEVIR +T  IEREINSVNDNPLIDVSR
Sbjct: 327 KAAEKQHEMDPLQKPKQDRYALRTSPQWLGPQIEVIRSSTKMIEREINSVNDNPLIDVSR 386

Query: 361 DVAVHGGNFQGTPIGVSMDNLRIAIAAIGKLMFAQFSELVCHHYNNGLPSNLSGGPNPSL 420
           + A+HGGNFQGTPIGVSMDN R+AIAAIGKLMFAQFSELV   YNNGLPSNLSGG NPSL
Sbjct: 387 NKAIHGGNFQGTPIGVSMDNTRLAIAAIGKLMFAQFSELVNDFYNNGLPSNLSGGRNPSL 446

Query: 421 DYGFKGAEIAMAAYCSELQYLANPVTTHVQSAEQHNQDVNSLGLISARKSAEAIEILKLM 480
           DYGFKGAEIAMA+YCSELQ+L NPVT HVQSAEQHNQDVNSLGLIS+RK+AEA+EILKLM
Sbjct: 447 DYGFKGAEIAMASYCSELQFLGNPVTNHVQSAEQHNQDVNSLGLISSRKTAEAVEILKLM 506

Query: 481 SATYMVALCQAIDLRQFEENMREVVKNALLQTIRKTLYMAEYGSLLESRFCEKDLLQVIE 540
           S T++V LCQA+DLR  EEN++  VKN + Q  +K L M   G L  SRFCE DLL+V++
Sbjct: 507 STTFLVGLCQAVDLRHLEENLKSTVKNTVSQVAKKVLTMGVNGELHPSRFCELDLLRVVD 566

Query: 541 REEVFSYIDDPTNTSYTLIPKLLEVLVEKALKGVATGKTDNGNETGIAIFKQIPEFLEEL 600
           RE +F+YIDDP + +Y L+ KL +VLVE ALK    G+T+    T  +IF++I  F +EL
Sbjct: 567 REYIFAYIDDPCSATYPLMQKLRQVLVEHALK---NGETEKNLST--SIFQKIAAFEDEL 626

Query: 601 KAKLEKDVENARGRFENGDFEIENRIKKCSTYPIYRFVRNEVGTQLLSGAKKVSPGEDIE 660
           KA L K+VE+AR   E+G+  I NRIK+C +YP+Y+F+R E+GT  L+G K  SPGE+ +
Sbjct: 627 KALLPKEVESARAVVESGNPAIPNRIKECRSYPLYKFIREELGTVYLTGEKVTSPGEEFD 686

Query: 661 KVVEAIDEGKLGNILMECLKN 676
           KV  A+ +G++ + L+ CL++
Sbjct: 687 KVFTAMSKGEIIDPLLACLES 699

BLAST of Cucsa.155520 vs. Swiss-Prot
Match: PAL2_ORYSI (Phenylalanine ammonia-lyase OS=Oryza sativa subsp. indica GN=ZB8 PE=3 SV=1)

HSP 1 Score: 810.8 bits (2093), Expect = 1.1e-233
Identity = 427/676 (63.17%), Postives = 522/676 (77.22%), Query Frame = 1

Query: 4   LSQTHFEEVCRMLSEFQEAQTVDVEGTTLTVAQVATIARRSDVKVILNESVAHDRVAESA 63
           ++ +H +EV RM++E+++   V +EG +L +AQVA +A   + +V L+ES A +RV  S+
Sbjct: 35  MTGSHLDEVKRMVAEYRQP-LVKIEGASLRIAQVAAVAAAGEARVELDES-ARERVKASS 94

Query: 64  KWVAEKVSSGIDIYGVTTGFGATSHLRTTKISDLQTELIRFLNAGVIGKDN----LPSCY 123
            WV   + +G D YGVTTGFGATSH RT +   LQ ELIRFLNAG  G       LP+  
Sbjct: 95  DWVMNSMMNGTDSYGVTTGFGATSHRRTKEGGALQRELIRFLNAGAFGTGTDGHVLPAEA 154

Query: 124 SKVAMLVRTNTLMQGYSGIRWEILEALVKLMNENLIPKLPLRGTITASGDLVPLSYIAGV 183
           ++ AMLVR NTL+QGYSGIR+EILEA+ KL+N N+ P LPLRGTITASGDLVPLSYIAG+
Sbjct: 155 TRAAMLVRINTLLQGYSGIRFEILEAIAKLLNANVTPCLPLRGTITASGDLVPLSYIAGL 214

Query: 184 VTGRHNSKVTTIEGDEITAIEALKRAGVAGPF-ELQAKEGLALVNGTAVGSAVAATVCYD 243
           VTGR N+     +G ++ A EA K AG+ G F ELQ KEGLA+VNGTAVGS +A+TV ++
Sbjct: 215 VTGRENAVAVAPDGSKVNAAEAFKIAGIQGGFFELQPKEGLAMVNGTAVGSGLASTVLFE 274

Query: 244 ANILALLASVLSALFCEAMLGKPEFTDPLTHKLKHHPGQIESAAVMEFLLNQSDFMKEAK 303
           ANILA+LA VLSA+FCE M GKPE+TD LTHKLKHHPGQIE+AA+ME +L  S +MK AK
Sbjct: 275 ANILAILAEVLSAVFCEVMNGKPEYTDHLTHKLKHHPGQIEAAAIMEHILEGSSYMKHAK 334

Query: 304 ICNENDPLTKPKQDRYALRTSPQWLGPQIEVIRMATHSIEREINSVNDNPLIDVSRDVAV 363
              E DPL KPKQDRYALRTSPQWLGPQIEVIR AT SIEREINSVNDNPLIDVSR  A+
Sbjct: 335 KLGELDPLMKPKQDRYALRTSPQWLGPQIEVIRAATKSIEREINSVNDNPLIDVSRGKAL 394

Query: 364 HGGNFQGTPIGVSMDNLRIAIAAIGKLMFAQFSELVCHHYNNGLPSNLSGGPNPSLDYGF 423
           HGGNFQGTPIGVSMDN R+AIAAIGKLMFAQFSELV   YNNGLPSNLSGG NPSLDYGF
Sbjct: 395 HGGNFQGTPIGVSMDNTRLAIAAIGKLMFAQFSELVNDFYNNGLPSNLSGGRNPSLDYGF 454

Query: 424 KGAEIAMAAYCSELQYLANPVTTHVQSAEQHNQDVNSLGLISARKSAEAIEILKLMSATY 483
           KGAEIAMA+YCSELQ+L NPVT HVQSAEQHNQDVNSLGLIS+RK+AEAI+ILKLMS+T+
Sbjct: 455 KGAEIAMASYCSELQFLGNPVTNHVQSAEQHNQDVNSLGLISSRKTAEAIDILKLMSSTF 514

Query: 484 MVALCQAIDLRQFEENMREVVKNALLQTIRKTLYMAEYGSLLESRFCEKDLLQVIEREEV 543
           ++ALCQA+DLR  EEN++  VK+ ++   +KTL     G L  +RFCEKDLL+ I+RE V
Sbjct: 515 LIALCQAVDLRHIEENVKSAVKSCVMTVAKKTLSTNSTGDLHVARFCEKDLLKEIDREAV 574

Query: 544 FSYIDDPTNTSYTLIPKLLEVLVEKALKGVATGKTDNGNETGIAIFKQIPEFLEELKAKL 603
           F+Y DDP + +Y L+ KL  VLVE+AL   A G  +   +T  ++F ++ +F EEL+A L
Sbjct: 575 FAYADDPCSHNYPLMKKLRNVLVERAL---ANGAAEFNADT--SVFAKVAQFEEELRATL 634

Query: 604 EKDVENARGRFENGDFEIENRIKKCSTYPIYRFVRNEVGTQLLSGAKKVSPGEDIEKVVE 663
              +E AR   ENG   I +RI +C +YP+YRFVR E+GT+ L+G K  SPGE++ KV+ 
Sbjct: 635 PGAIEAARAAVENGTAAIPSRITECRSYPLYRFVREELGTKYLTGEKTRSPGEELNKVLV 694

Query: 664 AIDEGKLGNILMECLK 675
           AI+EGK  + L+ECLK
Sbjct: 695 AINEGKHIDPLLECLK 703

BLAST of Cucsa.155520 vs. TrEMBL
Match: A0A0A0LYZ4_CUCSA (Phenylalanine ammonia-lyase OS=Cucumis sativus GN=Csa_1G590300 PE=3 SV=1)

HSP 1 Score: 1320.1 bits (3415), Expect = 0.0e+00
Identity = 675/675 (100.00%), Postives = 675/675 (100.00%), Query Frame = 1

Query: 1   ANALSQTHFEEVCRMLSEFQEAQTVDVEGTTLTVAQVATIARRSDVKVILNESVAHDRVA 60
           ANALSQTHFEEVCRMLSEFQEAQTVDVEGTTLTVAQVATIARRSDVKVILNESVAHDRVA
Sbjct: 37  ANALSQTHFEEVCRMLSEFQEAQTVDVEGTTLTVAQVATIARRSDVKVILNESVAHDRVA 96

Query: 61  ESAKWVAEKVSSGIDIYGVTTGFGATSHLRTTKISDLQTELIRFLNAGVIGKDNLPSCYS 120
           ESAKWVAEKVSSGIDIYGVTTGFGATSHLRTTKISDLQTELIRFLNAGVIGKDNLPSCYS
Sbjct: 97  ESAKWVAEKVSSGIDIYGVTTGFGATSHLRTTKISDLQTELIRFLNAGVIGKDNLPSCYS 156

Query: 121 KVAMLVRTNTLMQGYSGIRWEILEALVKLMNENLIPKLPLRGTITASGDLVPLSYIAGVV 180
           KVAMLVRTNTLMQGYSGIRWEILEALVKLMNENLIPKLPLRGTITASGDLVPLSYIAGVV
Sbjct: 157 KVAMLVRTNTLMQGYSGIRWEILEALVKLMNENLIPKLPLRGTITASGDLVPLSYIAGVV 216

Query: 181 TGRHNSKVTTIEGDEITAIEALKRAGVAGPFELQAKEGLALVNGTAVGSAVAATVCYDAN 240
           TGRHNSKVTTIEGDEITAIEALKRAGVAGPFELQAKEGLALVNGTAVGSAVAATVCYDAN
Sbjct: 217 TGRHNSKVTTIEGDEITAIEALKRAGVAGPFELQAKEGLALVNGTAVGSAVAATVCYDAN 276

Query: 241 ILALLASVLSALFCEAMLGKPEFTDPLTHKLKHHPGQIESAAVMEFLLNQSDFMKEAKIC 300
           ILALLASVLSALFCEAMLGKPEFTDPLTHKLKHHPGQIESAAVMEFLLNQSDFMKEAKIC
Sbjct: 277 ILALLASVLSALFCEAMLGKPEFTDPLTHKLKHHPGQIESAAVMEFLLNQSDFMKEAKIC 336

Query: 301 NENDPLTKPKQDRYALRTSPQWLGPQIEVIRMATHSIEREINSVNDNPLIDVSRDVAVHG 360
           NENDPLTKPKQDRYALRTSPQWLGPQIEVIRMATHSIEREINSVNDNPLIDVSRDVAVHG
Sbjct: 337 NENDPLTKPKQDRYALRTSPQWLGPQIEVIRMATHSIEREINSVNDNPLIDVSRDVAVHG 396

Query: 361 GNFQGTPIGVSMDNLRIAIAAIGKLMFAQFSELVCHHYNNGLPSNLSGGPNPSLDYGFKG 420
           GNFQGTPIGVSMDNLRIAIAAIGKLMFAQFSELVCHHYNNGLPSNLSGGPNPSLDYGFKG
Sbjct: 397 GNFQGTPIGVSMDNLRIAIAAIGKLMFAQFSELVCHHYNNGLPSNLSGGPNPSLDYGFKG 456

Query: 421 AEIAMAAYCSELQYLANPVTTHVQSAEQHNQDVNSLGLISARKSAEAIEILKLMSATYMV 480
           AEIAMAAYCSELQYLANPVTTHVQSAEQHNQDVNSLGLISARKSAEAIEILKLMSATYMV
Sbjct: 457 AEIAMAAYCSELQYLANPVTTHVQSAEQHNQDVNSLGLISARKSAEAIEILKLMSATYMV 516

Query: 481 ALCQAIDLRQFEENMREVVKNALLQTIRKTLYMAEYGSLLESRFCEKDLLQVIEREEVFS 540
           ALCQAIDLRQFEENMREVVKNALLQTIRKTLYMAEYGSLLESRFCEKDLLQVIEREEVFS
Sbjct: 517 ALCQAIDLRQFEENMREVVKNALLQTIRKTLYMAEYGSLLESRFCEKDLLQVIEREEVFS 576

Query: 541 YIDDPTNTSYTLIPKLLEVLVEKALKGVATGKTDNGNETGIAIFKQIPEFLEELKAKLEK 600
           YIDDPTNTSYTLIPKLLEVLVEKALKGVATGKTDNGNETGIAIFKQIPEFLEELKAKLEK
Sbjct: 577 YIDDPTNTSYTLIPKLLEVLVEKALKGVATGKTDNGNETGIAIFKQIPEFLEELKAKLEK 636

Query: 601 DVENARGRFENGDFEIENRIKKCSTYPIYRFVRNEVGTQLLSGAKKVSPGEDIEKVVEAI 660
           DVENARGRFENGDFEIENRIKKCSTYPIYRFVRNEVGTQLLSGAKKVSPGEDIEKVVEAI
Sbjct: 637 DVENARGRFENGDFEIENRIKKCSTYPIYRFVRNEVGTQLLSGAKKVSPGEDIEKVVEAI 696

Query: 661 DEGKLGNILMECLKN 676
           DEGKLGNILMECLKN
Sbjct: 697 DEGKLGNILMECLKN 711

BLAST of Cucsa.155520 vs. TrEMBL
Match: A0A0H4CUQ9_BETPL (Phenylalanine ammonia-lyase OS=Betula platyphylla GN=PAL1 PE=2 SV=1)

HSP 1 Score: 1071.2 bits (2769), Expect = 5.1e-310
Identity = 537/673 (79.79%), Postives = 605/673 (89.90%), Query Frame = 1

Query: 1   ANALSQTHFEEVCRMLSEFQEAQTVDVEGTTLTVAQVATIARRSDVKVILNESVAHDRVA 60
           A AL  THF+EVCRM+S+F + Q+VD++GT+LTVAQV  IARRS+VKV L+E+ A +RVA
Sbjct: 48  AEALQCTHFDEVCRMVSQFAKVQSVDIQGTSLTVAQVTAIARRSEVKVNLDEAAARERVA 107

Query: 61  ESAKWVAEKVSSGIDIYGVTTGFGATSHLRTTKISDLQTELIRFLNAGVIGKDNLPSCYS 120
           +SA WVAE +S G D YGVTTGFGATSH RT K  DLQTELIRFLNAGVIGK++LPS YS
Sbjct: 108 KSANWVAENISRGTDTYGVTTGFGATSHRRTNKTVDLQTELIRFLNAGVIGKESLPSTYS 167

Query: 121 KVAMLVRTNTLMQGYSGIRWEILEALVKLMNENLIPKLPLRGTITASGDLVPLSYIAGVV 180
           KVAMLVRTNTLMQG+SGIRWEILEA+  LMN+NLIPKLPLRGTITASGDLVPLSYIAG++
Sbjct: 168 KVAMLVRTNTLMQGFSGIRWEILEAMANLMNKNLIPKLPLRGTITASGDLVPLSYIAGLL 227

Query: 181 TGRHNSKVTTIEGDEITAIEALKRAGVAGPFELQAKEGLALVNGTAVGSAVAATVCYDAN 240
           TGRHNSKV T E +EITA+EALKRAG+  PFEL+AKEGLALVNGTAVGSAVAATVC+DAN
Sbjct: 228 TGRHNSKVVTPEDEEITALEALKRAGIPAPFELKAKEGLALVNGTAVGSAVAATVCFDAN 287

Query: 241 ILALLASVLSALFCEAMLGKPEFTDPLTHKLKHHPGQIESAAVMEFLLNQSDFMKEAKIC 300
           ILAL A +LSALFCE MLG  EFTDPLTH+LKHHPGQIE+AA+MEFLL++SD+MKEAK+ 
Sbjct: 288 ILALFAEILSALFCEVMLGNAEFTDPLTHELKHHPGQIEAAAIMEFLLDESDYMKEAKLR 347

Query: 301 NENDPLTKPKQDRYALRTSPQWLGPQIEVIRMATHSIEREINSVNDNPLIDVSRDVAVHG 360
            E DPLTKPKQDRYALRTSPQWLGPQIEVIRMATHSIEREINSVNDNPLIDV+RD+A+HG
Sbjct: 348 YEKDPLTKPKQDRYALRTSPQWLGPQIEVIRMATHSIEREINSVNDNPLIDVARDIALHG 407

Query: 361 GNFQGTPIGVSMDNLRIAIAAIGKLMFAQFSELVCHHYNNGLPSNLSGGPNPSLDYGFKG 420
           GNFQGTPIGVSMDNLRIA+AAIGKLMFAQFSELVC +YNNGLPSNLSGGPNPSLDYGFKG
Sbjct: 408 GNFQGTPIGVSMDNLRIAVAAIGKLMFAQFSELVCDYYNNGLPSNLSGGPNPSLDYGFKG 467

Query: 421 AEIAMAAYCSELQYLANPVTTHVQSAEQHNQDVNSLGLISARKSAEAIEILKLMSATYMV 480
           AEIAMA+YCSELQYLANPVTTHVQSAEQHNQDVNSLGLISARKSAEAIEILKLM ATYMV
Sbjct: 468 AEIAMASYCSELQYLANPVTTHVQSAEQHNQDVNSLGLISARKSAEAIEILKLMVATYMV 527

Query: 481 ALCQAIDLRQFEENMREVVKNALLQTIRKTLYMAEYGSLLESRFCEKDLLQVIEREEVFS 540
            LCQAIDLR  EENM+EVVK+ +LQ +RKTLY AE GSLLESRFCEK+LLQVIE + VF+
Sbjct: 528 GLCQAIDLRHLEENMKEVVKHVVLQVVRKTLYTAEDGSLLESRFCEKELLQVIEHQPVFN 587

Query: 541 YIDDPTNTSYTLIPKLLEVLVEKALKGVATGKTDNGNETGIAIFKQIPEFLEELKAKLEK 600
           Y+DDPT++SY L+PKL EVLVEKALK     KT+NG++ G +IFK+IP FL+ELKA+L +
Sbjct: 588 YLDDPTSSSYALLPKLREVLVEKALK--EHPKTENGHDEGFSIFKRIPVFLDELKARLGE 647

Query: 601 DVENARGRFENGDFEIENRIKKCSTYPIYRFVRNEVGTQLLSGAKKVSPGEDIEKVVEAI 660
           +V  AR RFE GDF + NRIKKC TYPIYRFVR EVGT LL+GAKKVSPGEDIEKV EAI
Sbjct: 648 EVVKARDRFEKGDFPMPNRIKKCRTYPIYRFVRTEVGTTLLTGAKKVSPGEDIEKVYEAI 707

Query: 661 DEGKLGNILMECL 674
           + GKLG+++++CL
Sbjct: 708 NGGKLGDVVVKCL 718

BLAST of Cucsa.155520 vs. TrEMBL
Match: A0A067KCD4_JATCU (Phenylalanine ammonia-lyase OS=Jatropha curcas GN=JCGZ_07452 PE=3 SV=1)

HSP 1 Score: 1062.8 bits (2747), Expect = 1.8e-307
Identity = 530/675 (78.52%), Postives = 603/675 (89.33%), Query Frame = 1

Query: 1   ANALSQTHFEEVCRMLSEFQEAQTVDVEGTTLTVAQVATIARRSDVKVILNESVAHDRVA 60
           A +L  +HF+EV  M S+F  AQTVD++GT+LTVA+V  +ARRS+V V LNE+VA DRV 
Sbjct: 23  AESLQSSHFDEVYEMASQFANAQTVDIQGTSLTVAEVTAVARRSNVSVQLNETVAGDRVR 82

Query: 61  ESAKWVAEKVSSGIDIYGVTTGFGATSHLRTTKISDLQTELIRFLNAGVIGKDNLPSCYS 120
           +SA WV+  +S GIDIYGVTTGFGATSH RTTK +DLQTELIRFLNAGVIGK+NLP  YS
Sbjct: 83  QSADWVSFNISHGIDIYGVTTGFGATSHRRTTKTADLQTELIRFLNAGVIGKENLPLSYS 142

Query: 121 KVAMLVRTNTLMQGYSGIRWEILEALVKLMNENLIPKLPLRGTITASGDLVPLSYIAGVV 180
           K AMLVRTNTLMQGYSGIRWEILE++ KLMN NLIPKLPLRGTITASGDLVPLSYIAG++
Sbjct: 143 KAAMLVRTNTLMQGYSGIRWEILESISKLMNLNLIPKLPLRGTITASGDLVPLSYIAGLL 202

Query: 181 TGRHNSKVTTIEGDEITAIEALKRAGVAGPFELQAKEGLALVNGTAVGSAVAATVCYDAN 240
           TGRHNSKV T++G+EIT +EALKRAG+  PFELQAKEGLALVNGTAVGSAVAATVC+DAN
Sbjct: 203 TGRHNSKVVTVQGEEITGLEALKRAGIEAPFELQAKEGLALVNGTAVGSAVAATVCFDAN 262

Query: 241 ILALLASVLSALFCEAMLGKPEFTDPLTHKLKHHPGQIESAAVMEFLLNQSDFMKEAKIC 300
           ILA LA +LSALFCE M GKPE+TDPLTH+LKHHPGQIE+AA+M+ LL++SD+MKEAKI 
Sbjct: 263 ILAFLAEILSALFCEVMHGKPEYTDPLTHQLKHHPGQIEAAAIMKHLLDESDYMKEAKIR 322

Query: 301 NENDPLTKPKQDRYALRTSPQWLGPQIEVIRMATHSIEREINSVNDNPLIDVSRDVAVHG 360
           NE DPLTKPKQDRYA+RTSPQWLGPQIEVIR ATHSIEREINSVNDNP+IDVSRD+A+HG
Sbjct: 323 NEKDPLTKPKQDRYAVRTSPQWLGPQIEVIRAATHSIEREINSVNDNPIIDVSRDLALHG 382

Query: 361 GNFQGTPIGVSMDNLRIAIAAIGKLMFAQFSELVCHHYNNGLPSNLSGGPNPSLDYGFKG 420
           GNFQGTPIGVSMDNLRIAIAAIGKLMFAQFSELVC +YNNGLPSNLSGGPNPSLDYG KG
Sbjct: 383 GNFQGTPIGVSMDNLRIAIAAIGKLMFAQFSELVCDYYNNGLPSNLSGGPNPSLDYGLKG 442

Query: 421 AEIAMAAYCSELQYLANPVTTHVQSAEQHNQDVNSLGLISARKSAEAIEILKLMSATYMV 480
           AEIAMA+YCSELQ+LANPVT+HVQSAEQHNQDVNSLGLISARKSAEAIEILKLMSAT+MV
Sbjct: 443 AEIAMASYCSELQFLANPVTSHVQSAEQHNQDVNSLGLISARKSAEAIEILKLMSATFMV 502

Query: 481 ALCQAIDLRQFEENMREVVKNALLQTIRKTLYMAEYGSLLESRFCEKDLLQVIEREEVFS 540
           ALCQAIDLR  EENMREV+K  + Q IRKTLY++E GSLLESRFCEK++LQV+E + VFS
Sbjct: 503 ALCQAIDLRHLEENMREVIKQVVRQVIRKTLYISEDGSLLESRFCEKEILQVVEHQPVFS 562

Query: 541 YIDDPTNTSYTLIPKLLEVLVEKALKGVATGKTDNGNETGIAIFKQIPEFLEELKAKLEK 600
           Y+DDPTN SY  + KL EVLV+KALK   +G   N ++ G  IFK+IP F+EEL+A+LE+
Sbjct: 563 YLDDPTNPSYPFLAKLQEVLVQKALKDPKSG---NSDQNGYTIFKRIPVFMEELRARLEE 622

Query: 601 DVENARGRFENGDFEIENRIKKCSTYPIYRFVRNEVGTQLLSGAKKVSPGEDIEKVVEAI 660
           DV  AR RF+ G+F +ENRIKKC TYPIY+FVR+EVGT+LLSGAKKVSPGEDIEKV EAI
Sbjct: 623 DVPKARERFDGGEFGVENRIKKCRTYPIYKFVRSEVGTELLSGAKKVSPGEDIEKVHEAI 682

Query: 661 DEGKLGNILMECLKN 676
           +EGKLG +LM+CLK+
Sbjct: 683 NEGKLGEVLMKCLKD 694

BLAST of Cucsa.155520 vs. TrEMBL
Match: A0A059CW46_EUCGR (Phenylalanine ammonia-lyase OS=Eucalyptus grandis GN=EUGRSUZ_C03570 PE=3 SV=1)

HSP 1 Score: 1057.0 bits (2732), Expect = 9.8e-306
Identity = 526/675 (77.93%), Postives = 604/675 (89.48%), Query Frame = 1

Query: 1   ANALSQTHFEEVCRMLSEFQEAQTVDVEGTTLTVAQVATIARRSDVKVILNESVAHDRVA 60
           A +L  THFEEV  +LS+F+ A+ VD+EGT L+VAQV  +ARRS+V+V LNE VA DRV+
Sbjct: 29  AQSLRCTHFEEVRSLLSQFENARIVDIEGTALSVAQVTAVARRSEVEVQLNEGVARDRVS 88

Query: 61  ESAKWVAEKVSSGIDIYGVTTGFGATSHLRTTKISDLQTELIRFLNAGVIGKDNLPSCYS 120
            SA WVAE +S G DIYGVTTGFG+TS+ RTTK SDLQTELIRFLNAGVIG++NLP  Y+
Sbjct: 89  RSANWVAETISCGRDIYGVTTGFGSTSNRRTTKTSDLQTELIRFLNAGVIGRENLPPSYA 148

Query: 121 KVAMLVRTNTLMQGYSGIRWEILEALVKLMNENLIPKLPLRGTITASGDLVPLSYIAGVV 180
           K AMLVR NTLMQGYSGIRWEIL+A+ KLMN+NLIPK+PLRGTITASGDLVPLSYIAG++
Sbjct: 149 KAAMLVRANTLMQGYSGIRWEILDAMAKLMNQNLIPKMPLRGTITASGDLVPLSYIAGLL 208

Query: 181 TGRHNSKVTTIEGDEITAIEALKRAGVAGPFELQAKEGLALVNGTAVGSAVAATVCYDAN 240
           TGRHNSKV T EG+EI++ EALKRAG++GPFELQAKEGLALVNGTAVGSAVAATVC+DAN
Sbjct: 209 TGRHNSKVVTPEGEEISSAEALKRAGISGPFELQAKEGLALVNGTAVGSAVAATVCFDAN 268

Query: 241 ILALLASVLSALFCEAMLGKPEFTDPLTHKLKHHPGQIESAAVMEFLLNQSDFMKEAKIC 300
           ILALLA  LSA+FCEAM GKPEFTDPLTH LKHHPGQIE+A++ME+LLN S++MKEAK+ 
Sbjct: 269 ILALLAETLSAMFCEAMHGKPEFTDPLTHLLKHHPGQIEAASLMEYLLNGSEYMKEAKLR 328

Query: 301 NENDPLTKPKQDRYALRTSPQWLGPQIEVIRMATHSIEREINSVNDNPLIDVSRDVAVHG 360
           +E DPLTKPKQDRYALRTSPQWLGPQ+EVIR ATHSIEREINSVNDNPLIDV+RD+A+HG
Sbjct: 329 HEKDPLTKPKQDRYALRTSPQWLGPQVEVIRAATHSIEREINSVNDNPLIDVARDLALHG 388

Query: 361 GNFQGTPIGVSMDNLRIAIAAIGKLMFAQFSELVCHHYNNGLPSNLSGGPNPSLDYGFKG 420
           GNFQGTPIGVSMDNLRIA+AAIGKL+FAQFSELVC +YNNGLPSNLS GPNPSLDYG KG
Sbjct: 389 GNFQGTPIGVSMDNLRIALAAIGKLLFAQFSELVCDYYNNGLPSNLSAGPNPSLDYGLKG 448

Query: 421 AEIAMAAYCSELQYLANPVTTHVQSAEQHNQDVNSLGLISARKSAEAIEILKLMSATYMV 480
           AEIAMAAYCSELQYLANPVTTHVQSAEQHNQDVNSLGLISARKSAEAI+ILKLMSATYMV
Sbjct: 449 AEIAMAAYCSELQYLANPVTTHVQSAEQHNQDVNSLGLISARKSAEAIDILKLMSATYMV 508

Query: 481 ALCQAIDLRQFEENMREVVKNALLQTIRKTLYMAEYGSLLESRFCEKDLLQVIEREEVFS 540
           ALCQAIDLR  EENMRE VK ++LQ ++KTLYM+E G LLESRFCEK+LLQV+E E VFS
Sbjct: 509 ALCQAIDLRHLEENMREAVKRSVLQAVKKTLYMSEDGLLLESRFCEKELLQVVEHEPVFS 568

Query: 541 YIDDPTNTSYTLIPKLLEVLVEKALKGVATGKTDNGNETGIAIFKQIPEFLEELKAKLEK 600
           Y+DDP N SY L+PKL EVLV +ALK     KT++ +E G +IFK+IP F+EELKA+LE+
Sbjct: 569 YLDDPANPSYVLLPKLQEVLVTRALK---DPKTEDASENGYSIFKRIPIFIEELKARLEE 628

Query: 601 DVENARGRFENGDFEIENRIKKCSTYPIYRFVRNEVGTQLLSGAKKVSPGEDIEKVVEAI 660
           +V  AR RF++G+F +ENRIK C TYP+YRFVR+++ TQLLSGAKKVSPGE+IEKV EAI
Sbjct: 629 EVPKARERFDDGEFGVENRIKNCRTYPVYRFVRSDMRTQLLSGAKKVSPGEEIEKVYEAI 688

Query: 661 DEGKLGNILMECLKN 676
           +EGKLG++LM+C KN
Sbjct: 689 NEGKLGDVLMQCFKN 700

BLAST of Cucsa.155520 vs. TrEMBL
Match: E7BLC8_BETPL (Phenylalanine ammonia-lyase OS=Betula platyphylla GN=PAL PE=2 SV=1)

HSP 1 Score: 1056.2 bits (2730), Expect = 1.7e-305
Identity = 534/675 (79.11%), Postives = 601/675 (89.04%), Query Frame = 1

Query: 1   ANALSQTHFEEVCRMLSEFQEAQTVDVEGTTLTVAQVATIARRSDVKVILNESVAHDRVA 60
           A AL  THF+EVCRM+S+F + Q+VD++GT+LTVAQV  IARRS+VKV L+E+ A +RVA
Sbjct: 48  AEALQCTHFDEVCRMVSQFAKVQSVDIQGTSLTVAQVTAIARRSEVKVNLDEAAARERVA 107

Query: 61  ESAKWVAEKVSSGIDIYGVTTGFGATSHLRTTKISDLQTELIRFLNAGVIGKDNLPSCYS 120
           +SA WVAE +S G D YGVTTGFGATSH RT K  DLQTELIRFLNAGVIGK++LPS YS
Sbjct: 108 KSANWVAENISRGTDTYGVTTGFGATSHRRTNKTVDLQTELIRFLNAGVIGKESLPSTYS 167

Query: 121 KVAMLVRTN--TLMQGYSGIRWEILEALVKLMNENLIPKLPLRGTITASGDLVPLSYIAG 180
           KVAMLVRTN  T  +G+SGIRWEILEA+  LMN+NLIPKLPLRGTITASGDLVPLSYIAG
Sbjct: 168 KVAMLVRTNEHTHARGFSGIRWEILEAMANLMNKNLIPKLPLRGTITASGDLVPLSYIAG 227

Query: 181 VVTGRHNSKVTTIEGDEITAIEALKRAGVAGPFELQAKEGLALVNGTAVGSAVAATVCYD 240
           ++TGRHNSKV T E +EITA+EALKRAG+  PFELQAKEGLALVNGTAVGSAVAATVC+D
Sbjct: 228 LLTGRHNSKVVTPEDEEITALEALKRAGIPAPFELQAKEGLALVNGTAVGSAVAATVCFD 287

Query: 241 ANILALLASVLSALFCEAMLGKPEFTDPLTHKLKHHPGQIESAAVMEFLLNQSDFMKEAK 300
           ANILAL A +LSALFCE MLG  EFTDPLTH+LKHHPGQIE+AA MEFLL++SD+MKEAK
Sbjct: 288 ANILALFAEILSALFCEVMLGNAEFTDPLTHELKHHPGQIEAAATMEFLLDESDYMKEAK 347

Query: 301 ICNENDPLTKPKQDRYALRTSPQWLGPQIEVIRMATHSIEREINSVNDNPLIDVSRDVAV 360
           +  E DPLTKPKQDRYALRTSPQWLGPQIEVIRMATHSIEREINSVNDNPLIDV+RD+A+
Sbjct: 348 LRYEKDPLTKPKQDRYALRTSPQWLGPQIEVIRMATHSIEREINSVNDNPLIDVARDIAL 407

Query: 361 HGGNFQGTPIGVSMDNLRIAIAAIGKLMFAQFSELVCHHYNNGLPSNLSGGPNPSLDYGF 420
           HGGNFQGTPIGVSMDNLRIAIAAIGKLMFAQFSELVC +YNNGLPS+LSGGPNPSLDYGF
Sbjct: 408 HGGNFQGTPIGVSMDNLRIAIAAIGKLMFAQFSELVCDYYNNGLPSSLSGGPNPSLDYGF 467

Query: 421 KGAEIAMAAYCSELQYLANPVTTHVQSAEQHNQDVNSLGLISARKSAEAIEILKLMSATY 480
           KGAEIAMA+YCSELQYLANPVTTHVQSAEQHNQDVNSLGLISARKSAEAIEILKLM ATY
Sbjct: 468 KGAEIAMASYCSELQYLANPVTTHVQSAEQHNQDVNSLGLISARKSAEAIEILKLMVATY 527

Query: 481 MVALCQAIDLRQFEENMREVVKNALLQTIRKTLYMAEYGSLLESRFCEKDLLQVIEREEV 540
           MV LCQAIDLR  EENM+EVVK+ +LQ +RKTLY AE GSLLESRFCEK+LLQVIE + V
Sbjct: 528 MVGLCQAIDLRHLEENMKEVVKHVVLQVVRKTLYTAEDGSLLESRFCEKELLQVIEHQPV 587

Query: 541 FSYIDDPTNTSYTLIPKLLEVLVEKALKGVATGKTDNGNETGIAIFKQIPEFLEELKAKL 600
           F+Y+DDPT++SY L+PKL EVLVEKALK     KT+NG++ G +IFK+IP FL+ELKA+L
Sbjct: 588 FNYLDDPTSSSYALLPKLREVLVEKALK--EHPKTENGHDEGFSIFKRIPVFLDELKARL 647

Query: 601 EKDVENARGRFENGDFEIENRIKKCSTYPIYRFVRNEVGTQLLSGAKKVSPGEDIEKVVE 660
            ++V  AR RFE GDF + NRIKKC TYPIYRFVR EVGT LL+GAK VSPGEDIEKV E
Sbjct: 648 GEEVVKARDRFEKGDFPMPNRIKKCRTYPIYRFVRTEVGTTLLTGAKMVSPGEDIEKVYE 707

Query: 661 AIDEGKLGNILMECL 674
           AI+ GKLG+++++CL
Sbjct: 708 AINGGKLGDVVVKCL 720

BLAST of Cucsa.155520 vs. TAIR10
Match: AT3G53260.1 (AT3G53260.1 phenylalanine ammonia-lyase 2)

HSP 1 Score: 814.7 bits (2103), Expect = 4.3e-236
Identity = 428/681 (62.85%), Postives = 530/681 (77.83%), Query Frame = 1

Query: 1   ANALSQTHFEEVCRMLSEFQEAQTVDVEGTTLTVAQVATIAR-RSDVKVILNESVAHDRV 60
           A+ +  +H +EV +M+ E++    V++ G TLT+ QVA I+     VKV L E+ +   V
Sbjct: 34  ADQMKGSHLDEVKKMVEEYRRP-VVNLGGETLTIGQVAAISTVGGSVKVELAET-SRAGV 93

Query: 61  AESAKWVAEKVSSGIDIYGVTTGFGATSHLRTTKISDLQTELIRFLNAGVIGKDN----- 120
             S+ WV E ++ G D YGVTTGFGATSH RT   + LQTELIRFLNAG+ G        
Sbjct: 94  KASSDWVMESMNKGTDSYGVTTGFGATSHRRTKNGTALQTELIRFLNAGIFGNTKETCHT 153

Query: 121 LPSCYSKVAMLVRTNTLMQGYSGIRWEILEALVKLMNENLIPKLPLRGTITASGDLVPLS 180
           LP   ++ AMLVR NTL+QGYSGIR+EILEA+  L+N N+ P LPLRGTITASGDLVPLS
Sbjct: 154 LPQSATRAAMLVRVNTLLQGYSGIRFEILEAITSLLNHNISPSLPLRGTITASGDLVPLS 213

Query: 181 YIAGVVTGRHNSKVTTIEGDEITAIEALKRAGVA-GPFELQAKEGLALVNGTAVGSAVAA 240
           YIAG++TGR NSK T  +G+ +TA EA ++AG++ G F+LQ KEGLALVNGTAVGS +A+
Sbjct: 214 YIAGLLTGRPNSKATGPDGESLTAKEAFEKAGISTGFFDLQPKEGLALVNGTAVGSGMAS 273

Query: 241 TVCYDANILALLASVLSALFCEAMLGKPEFTDPLTHKLKHHPGQIESAAVMEFLLNQSDF 300
            V ++AN+ A+LA VLSA+F E M GKPEFTD LTH+LKHHPGQIE+AA+ME +L+ S +
Sbjct: 274 MVLFEANVQAVLAEVLSAIFAEVMSGKPEFTDHLTHRLKHHPGQIEAAAIMEHILDGSSY 333

Query: 301 MKEAKICNENDPLTKPKQDRYALRTSPQWLGPQIEVIRMATHSIEREINSVNDNPLIDVS 360
           MK A+  +E DPL KPKQDRYALRTSPQWLGPQIEVIR AT SIEREINSVNDNPLIDVS
Sbjct: 334 MKLAQKVHEMDPLQKPKQDRYALRTSPQWLGPQIEVIRQATKSIEREINSVNDNPLIDVS 393

Query: 361 RDVAVHGGNFQGTPIGVSMDNLRIAIAAIGKLMFAQFSELVCHHYNNGLPSNLSGGPNPS 420
           R+ A+HGGNFQGTPIGVSMDN R+AIAAIGKLMFAQFSELV   YNNGLPSNL+   NPS
Sbjct: 394 RNKAIHGGNFQGTPIGVSMDNTRLAIAAIGKLMFAQFSELVNDFYNNGLPSNLTASSNPS 453

Query: 421 LDYGFKGAEIAMAAYCSELQYLANPVTTHVQSAEQHNQDVNSLGLISARKSAEAIEILKL 480
           LDYGFKGAEIAMA+YCSELQYLANPVT+HVQSAEQHNQDVNSLGLIS+RK++EA++ILKL
Sbjct: 454 LDYGFKGAEIAMASYCSELQYLANPVTSHVQSAEQHNQDVNSLGLISSRKTSEAVDILKL 513

Query: 481 MSATYMVALCQAIDLRQFEENMREVVKNALLQTIRKTLYMAEYGSLLESRFCEKDLLQVI 540
           MS T++V +CQA+DLR  EEN+R+ VKN + Q  +K L     G L  SRFCEKDLL+V+
Sbjct: 514 MSTTFLVGICQAVDLRHLEENLRQTVKNTVSQVAKKVLTTGINGELHPSRFCEKDLLKVV 573

Query: 541 EREEVFSYIDDPTNTSYTLIPKLLEVLVEKALKGVATGKTDNGNETGIAIFKQIPEFLEE 600
           +RE+VF+Y+DDP + +Y L+ +L +V+V+ AL   + G+T+    T  +IF++I  F EE
Sbjct: 574 DREQVFTYVDDPCSATYPLMQRLRQVIVDHAL---SNGETEKNAVT--SIFQKIGAFEEE 633

Query: 601 LKAKLEKDVENARGRFENGDFEIENRIKKCSTYPIYRFVRNEVGTQLLSGAKKVSPGEDI 660
           LKA L K+VE AR  + NG   I NRIK+C +YP+YRFVR E+GT+LL+G K VSPGE+ 
Sbjct: 634 LKAVLPKEVEAARAAYGNGTAPIPNRIKECRSYPLYRFVREELGTKLLTGEKVVSPGEEF 693

Query: 661 EKVVEAIDEGKLGNILMECLK 675
           +KV  A+ EGKL + LM+CLK
Sbjct: 694 DKVFTAMCEGKLIDPLMDCLK 707

BLAST of Cucsa.155520 vs. TAIR10
Match: AT3G10340.1 (AT3G10340.1 phenylalanine ammonia-lyase 4)

HSP 1 Score: 807.4 bits (2084), Expect = 6.8e-234
Identity = 422/680 (62.06%), Postives = 516/680 (75.88%), Query Frame = 1

Query: 1   ANALSQTHFEEVCRMLSEFQEAQTVDVEGTTLTVAQVATIARRSDVKVILNESVAHDRVA 60
           A AL  +H +EV RM+ E+++ + V + G TLT+ QVA +AR      +     A   V 
Sbjct: 24  AEALKGSHLDEVKRMVKEYRK-EAVKLGGETLTIGQVAAVARGGGGSTVELAEEARAGVK 83

Query: 61  ESAKWVAEKVSSGIDIYGVTTGFGATSHLRTTKISDLQTELIRFLNAGVIG------KDN 120
            S++WV E ++ G D YGVTTGFGATSH RT +   LQ ELIRFLNAG+ G         
Sbjct: 84  ASSEWVMESMNRGTDSYGVTTGFGATSHRRTKQGGALQNELIRFLNAGIFGPGAGDTSHT 143

Query: 121 LPSCYSKVAMLVRTNTLMQGYSGIRWEILEALVKLMNENLIPKLPLRGTITASGDLVPLS 180
           LP   ++ AMLVR NTL+QGYSGIR+EILEA+ KL+N  + P LPLRGTITASGDLVPLS
Sbjct: 144 LPKPTTRAAMLVRVNTLLQGYSGIRFEILEAITKLLNHEITPCLPLRGTITASGDLVPLS 203

Query: 181 YIAGVVTGRHNSKVTTIEGDEITAIEALKRAGVAGPFELQAKEGLALVNGTAVGSAVAAT 240
           YIAG++TGR NSK     G+ +TA EA K AGV+  FELQ KEGLALVNGTAVGS +A+T
Sbjct: 204 YIAGLLTGRPNSKAVGPSGETLTASEAFKLAGVSSFFELQPKEGLALVNGTAVGSGLAST 263

Query: 241 VCYDANILALLASVLSALFCEAMLGKPEFTDPLTHKLKHHPGQIESAAVMEFLLNQSDFM 300
           V +DANILA+L+ V+SA+F E M GKPEFTD LTHKLKHHPGQIE+AA+ME +L+ S ++
Sbjct: 264 VLFDANILAVLSEVMSAMFAEVMQGKPEFTDHLTHKLKHHPGQIEAAAIMEHILDGSSYV 323

Query: 301 KEAKICNENDPLTKPKQDRYALRTSPQWLGPQIEVIRMATHSIEREINSVNDNPLIDVSR 360
           KEA++ +E DPL KPKQDRYALRTSPQWLGPQIEVIR AT  IEREINSVNDNPLIDVSR
Sbjct: 324 KEAQLLHEMDPLQKPKQDRYALRTSPQWLGPQIEVIRAATKMIEREINSVNDNPLIDVSR 383

Query: 361 DVAVHGGNFQGTPIGVSMDNLRIAIAAIGKLMFAQFSELVCHHYNNGLPSNLSGGPNPSL 420
           + A+HGGNFQGTPIGV+MDN R+AIA+IGKLMFAQFSELV   YNNGLPSNLSGG NPSL
Sbjct: 384 NKALHGGNFQGTPIGVAMDNSRLAIASIGKLMFAQFSELVNDFYNNGLPSNLSGGRNPSL 443

Query: 421 DYGFKGAEIAMAAYCSELQYLANPVTTHVQSAEQHNQDVNSLGLISARKSAEAIEILKLM 480
           DYGFKGAEIAMA+YCSELQ+LANPVT HVQSAEQHNQDVNSLGLIS+RK+AEA++ILKLM
Sbjct: 444 DYGFKGAEIAMASYCSELQFLANPVTNHVQSAEQHNQDVNSLGLISSRKTAEAVDILKLM 503

Query: 481 SATYMVALCQAIDLRQFEENMREVVKNALLQTIRKTLYMAEYGSLLESRFCEKDLLQVIE 540
           S TY+VALCQA+DLR  EEN+++ VK+A+ Q  ++ L +   G L  SRF E+D+LQV++
Sbjct: 504 STTYLVALCQAVDLRHLEENLKKAVKSAVSQVAKRVLTVGANGELHPSRFTERDVLQVVD 563

Query: 541 REEVFSYIDDPTNTSYTLIPKLLEVLVEKALKGVATGKTDNGNETGIAIFKQIPEFLEEL 600
           RE VFSY DDP + +Y L+ KL  +LV+ AL        +    +  ++F +I  F  EL
Sbjct: 564 REYVFSYADDPCSLTYPLMQKLRHILVDHAL-----ADPEREANSATSVFHKIGAFEAEL 623

Query: 601 KAKLEKDVENARGRFENGDFEIENRIKKCSTYPIYRFVRNEVGTQLLSGAKKVSPGEDIE 660
           K  L K+VE  R  +E G   I NRIK+C +YP+YRFVR+E+ T+LL+G    SPGE+ +
Sbjct: 624 KLLLPKEVERVRVEYEEGTSAIANRIKECRSYPLYRFVRDELNTELLTGENVRSPGEEFD 683

Query: 661 KVVEAIDEGKLGNILMECLK 675
           KV  AI +GKL + L+ECLK
Sbjct: 684 KVFLAISDGKLIDPLLECLK 697

BLAST of Cucsa.155520 vs. TAIR10
Match: AT2G37040.1 (AT2G37040.1 PHE ammonia lyase 1)

HSP 1 Score: 807.4 bits (2084), Expect = 6.8e-234
Identity = 426/680 (62.65%), Postives = 525/680 (77.21%), Query Frame = 1

Query: 1   ANALSQTHFEEVCRMLSEFQEAQTVDVEGTTLTVAQVATIAR-RSDVKVILNESVAHDRV 60
           A  +  +H +EV RM++EF++   V++ G TLT+ QVA I+   + VKV L+E+ A   V
Sbjct: 42  AEQMKGSHLDEVKRMVAEFRKP-VVNLGGETLTIGQVAAISTIGNSVKVELSET-ARAGV 101

Query: 61  AESAKWVAEKVSSGIDIYGVTTGFGATSHLRTTKISDLQTELIRFLNAGVIGKDN----- 120
             S+ WV E ++ G D YGVTTGFGATSH RT     LQ ELIRFLNAG+ G        
Sbjct: 102 NASSDWVMESMNKGTDSYGVTTGFGATSHRRTKNGVALQKELIRFLNAGIFGSTKETSHT 161

Query: 121 LPSCYSKVAMLVRTNTLMQGYSGIRWEILEALVKLMNENLIPKLPLRGTITASGDLVPLS 180
           LP   ++ AMLVR NTL+QG+SGIR+EILEA+   +N N+ P LPLRGTITASGDLVPLS
Sbjct: 162 LPHSATRAAMLVRINTLLQGFSGIRFEILEAITSFLNNNITPSLPLRGTITASGDLVPLS 221

Query: 181 YIAGVVTGRHNSKVTTIEGDEITAIEALKRAGVA-GPFELQAKEGLALVNGTAVGSAVAA 240
           YIAG++TGR NSK T   G+ +TA EA K AG++ G F+LQ KEGLALVNGTAVGS +A+
Sbjct: 222 YIAGLLTGRPNSKATGPNGEALTAEEAFKLAGISSGFFDLQPKEGLALVNGTAVGSGMAS 281

Query: 241 TVCYDANILALLASVLSALFCEAMLGKPEFTDPLTHKLKHHPGQIESAAVMEFLLNQSDF 300
            V ++ N+L++LA +LSA+F E M GKPEFTD LTH+LKHHPGQIE+AA+ME +L+ S +
Sbjct: 282 MVLFETNVLSVLAEILSAVFAEVMSGKPEFTDHLTHRLKHHPGQIEAAAIMEHILDGSSY 341

Query: 301 MKEAKICNENDPLTKPKQDRYALRTSPQWLGPQIEVIRMATHSIEREINSVNDNPLIDVS 360
           MK A+  +E DPL KPKQDRYALRTSPQWLGPQIEVIR AT SIEREINSVNDNPLIDVS
Sbjct: 342 MKLAQKLHEMDPLQKPKQDRYALRTSPQWLGPQIEVIRYATKSIEREINSVNDNPLIDVS 401

Query: 361 RDVAVHGGNFQGTPIGVSMDNLRIAIAAIGKLMFAQFSELVCHHYNNGLPSNLSGGPNPS 420
           R+ A+HGGNFQGTPIGVSMDN R+AIAAIGKLMFAQFSELV   YNNGLPSNL+   NPS
Sbjct: 402 RNKAIHGGNFQGTPIGVSMDNTRLAIAAIGKLMFAQFSELVNDFYNNGLPSNLTASRNPS 461

Query: 421 LDYGFKGAEIAMAAYCSELQYLANPVTTHVQSAEQHNQDVNSLGLISARKSAEAIEILKL 480
           LDYGFKGAEIAMA+YCSELQYLANPVT+HVQSAEQHNQDVNSLGLIS+RK++EA++ILKL
Sbjct: 462 LDYGFKGAEIAMASYCSELQYLANPVTSHVQSAEQHNQDVNSLGLISSRKTSEAVDILKL 521

Query: 481 MSATYMVALCQAIDLRQFEENMREVVKNALLQTIRKTLYMAEYGSLLESRFCEKDLLQVI 540
           MS T++VA+CQA+DLR  EEN+R+ VKN + Q  +K L     G L  SRFCEKDLL+V+
Sbjct: 522 MSTTFLVAICQAVDLRHLEENLRQTVKNTVSQVAKKVLTTGVNGELHPSRFCEKDLLKVV 581

Query: 541 EREEVFSYIDDPTNTSYTLIPKLLEVLVEKALKGVATGKTDNGNETGIAIFKQIPEFLEE 600
           +RE+V++Y DDP + +Y LI KL +V+V+ AL     G+++    T  +IF +I  F EE
Sbjct: 582 DREQVYTYADDPCSATYPLIQKLRQVIVDHAL---INGESEKNAVT--SIFHKIGAFEEE 641

Query: 601 LKAKLEKDVENARGRFENGDFEIENRIKKCSTYPIYRFVRNEVGTQLLSGAKKVSPGEDI 660
           LKA L K+VE AR  ++NG   I NRIK+C +YP+YRFVR E+GT+LL+G K  SPGE+ 
Sbjct: 642 LKAVLPKEVEAARAAYDNGTSAIPNRIKECRSYPLYRFVREELGTELLTGEKVTSPGEEF 701

Query: 661 EKVVEAIDEGKLGNILMECL 674
           +KV  AI EGK+ + +MECL
Sbjct: 702 DKVFTAICEGKIIDPMMECL 714

BLAST of Cucsa.155520 vs. TAIR10
Match: AT5G04230.2 (AT5G04230.2 phenyl alanine ammonia-lyase 3)

HSP 1 Score: 742.7 bits (1916), Expect = 2.1e-214
Identity = 400/686 (58.31%), Postives = 494/686 (72.01%), Query Frame = 1

Query: 1   ANALSQTHFEEVCRMLSEFQEAQTVDVEGTTLTVAQVATIARRSDVKVILNESVAHDRVA 60
           A AL  +H EEV +M+ ++++  TV + G TLT+ QVA +A       +  E  A   V 
Sbjct: 21  AEALKGSHLEEVKKMVKDYRKG-TVQLGGETLTIGQVAAVASGGPTVELSEE--ARGGVK 80

Query: 61  ESAKWVAEKVSSGIDIYGVTTGFGATSHLRTTKISDLQTELIRFLNAGVIGKDN------ 120
            S+ WV E ++   D YG+TTGFG++S  RT + + LQ ELIR+LNAG+    N      
Sbjct: 81  ASSDWVMESMNRDTDTYGITTGFGSSSRRRTDQGAALQKELIRYLNAGIFATGNEDDDRS 140

Query: 121 --LPSCYSKVAMLVRTNTLMQGYSGIRWEILEALVKLMNENLIPKLPLRGTITASGDLVP 180
             LP   ++ AML+R NTL+QGYSGIR+EILEA+  L+N  + P LPLRGTITASGDLVP
Sbjct: 141 NTLPRPATRAAMLIRVNTLLQGYSGIRFEILEAITTLLNCKITPLLPLRGTITASGDLVP 200

Query: 181 LSYIAGVVTGRHNSKVTTIEGDEITAIEALKRAGVAGPFELQAKEGLALVNGTAVGSAVA 240
           LSYIAG + GR NS+     G+ +TA+EA K AGV+  FEL+ KEGLALVNGTAVGSA+A
Sbjct: 201 LSYIAGFLIGRPNSRSVGPSGEILTALEAFKLAGVSSFFELRPKEGLALVNGTAVGSALA 260

Query: 241 ATVCYDANILALLASVLSALFCEAMLGKPEFTDPLTHKLKHHPGQIESAAVMEFLLNQSD 300
           +TV YDANIL + + V SA+F E M GKPEFTD LTHKLKHHPGQIE+AA+ME +L+ S 
Sbjct: 261 STVLYDANILVVFSEVASAMFAEVMQGKPEFTDHLTHKLKHHPGQIEAAAIMEHILDGSS 320

Query: 301 FMKEAKICNENDPLTKPKQDRY----ALRTSPQWLGPQIEVIRMATHSIEREINSVNDNP 360
           ++KEA   ++ DPL KPKQDRY    ALRTSPQWLGPQIEVIR AT  IEREINSVNDNP
Sbjct: 321 YVKEALHLHKIDPLQKPKQDRYVLGYALRTSPQWLGPQIEVIRAATKMIEREINSVNDNP 380

Query: 361 LIDVSRDVAVHGGNFQGTPIGVSMDNLRIAIAAIGKLMFAQFSELVCHHYNNGLPSNLSG 420
           LIDVSR+ A+HGGNFQGTPIGV+MDN R+A+A+IGKLMFAQF+ELV   YNNGLPSNLSG
Sbjct: 381 LIDVSRNKAIHGGNFQGTPIGVAMDNTRLALASIGKLMFAQFTELVNDFYNNGLPSNLSG 440

Query: 421 GPNPSLDYGFKGAEIAMAAYCSELQYLANPVTTHVQSAEQHNQDVNSLGLISARKSAEAI 480
           G NPSLDYG KGAE+AMA+YCSELQ+LANPVT HV+SA QHNQDVNSLGLIS+R +AEA+
Sbjct: 441 GRNPSLDYGLKGAEVAMASYCSELQFLANPVTNHVESASQHNQDVNSLGLISSRTTAEAV 500

Query: 481 EILKLMSATYMVALCQAIDLRQFEENMREVVKNALLQTIRKTLYMAEYGSLLESRFCEKD 540
            ILKLMS TY+VALCQA DLR  EE +++ V   +  T +  L +  +           D
Sbjct: 501 VILKLMSTTYLVALCQAFDLRHLEEILKKAVNEVVSHTAKSVLAIEPFRK-------HDD 560

Query: 541 LLQVIEREEVFSYIDDPTNTSYTLIPKLLEVLVEKALKGVATGKTDNGNETGIAIFKQIP 600
           +L V+ RE VFSY+DDP++ +  L+ KL  VL +KAL     G+TD        +F++I 
Sbjct: 561 ILGVVNREYVFSYVDDPSSLTNPLMQKLRHVLFDKAL-AEPEGETD-------TVFRKIG 620

Query: 601 EFLEELKAKLEKDVENARGRFENGDFEIENRIKKCSTYPIYRFVRNEVGTQLLSGAKKVS 660
            F  ELK  L K+VE  R  +ENG F + NRIKKC +YP+YRFVRNE+ T+LL+G    S
Sbjct: 621 AFEAELKFLLPKEVERVRTEYENGTFNVANRIKKCRSYPLYRFVRNELETRLLTGEDVRS 680

Query: 661 PGEDIEKVVEAIDEGKLGNILMECLK 675
           PGED +KV  AI +GKL + L ECLK
Sbjct: 681 PGEDFDKVFRAISQGKLIDPLFECLK 688

BLAST of Cucsa.155520 vs. NCBI nr
Match: gi|449452542|ref|XP_004144018.1| (PREDICTED: phenylalanine ammonia-lyase isoform X1 [Cucumis sativus])

HSP 1 Score: 1320.1 bits (3415), Expect = 0.0e+00
Identity = 675/675 (100.00%), Postives = 675/675 (100.00%), Query Frame = 1

Query: 1   ANALSQTHFEEVCRMLSEFQEAQTVDVEGTTLTVAQVATIARRSDVKVILNESVAHDRVA 60
           ANALSQTHFEEVCRMLSEFQEAQTVDVEGTTLTVAQVATIARRSDVKVILNESVAHDRVA
Sbjct: 37  ANALSQTHFEEVCRMLSEFQEAQTVDVEGTTLTVAQVATIARRSDVKVILNESVAHDRVA 96

Query: 61  ESAKWVAEKVSSGIDIYGVTTGFGATSHLRTTKISDLQTELIRFLNAGVIGKDNLPSCYS 120
           ESAKWVAEKVSSGIDIYGVTTGFGATSHLRTTKISDLQTELIRFLNAGVIGKDNLPSCYS
Sbjct: 97  ESAKWVAEKVSSGIDIYGVTTGFGATSHLRTTKISDLQTELIRFLNAGVIGKDNLPSCYS 156

Query: 121 KVAMLVRTNTLMQGYSGIRWEILEALVKLMNENLIPKLPLRGTITASGDLVPLSYIAGVV 180
           KVAMLVRTNTLMQGYSGIRWEILEALVKLMNENLIPKLPLRGTITASGDLVPLSYIAGVV
Sbjct: 157 KVAMLVRTNTLMQGYSGIRWEILEALVKLMNENLIPKLPLRGTITASGDLVPLSYIAGVV 216

Query: 181 TGRHNSKVTTIEGDEITAIEALKRAGVAGPFELQAKEGLALVNGTAVGSAVAATVCYDAN 240
           TGRHNSKVTTIEGDEITAIEALKRAGVAGPFELQAKEGLALVNGTAVGSAVAATVCYDAN
Sbjct: 217 TGRHNSKVTTIEGDEITAIEALKRAGVAGPFELQAKEGLALVNGTAVGSAVAATVCYDAN 276

Query: 241 ILALLASVLSALFCEAMLGKPEFTDPLTHKLKHHPGQIESAAVMEFLLNQSDFMKEAKIC 300
           ILALLASVLSALFCEAMLGKPEFTDPLTHKLKHHPGQIESAAVMEFLLNQSDFMKEAKIC
Sbjct: 277 ILALLASVLSALFCEAMLGKPEFTDPLTHKLKHHPGQIESAAVMEFLLNQSDFMKEAKIC 336

Query: 301 NENDPLTKPKQDRYALRTSPQWLGPQIEVIRMATHSIEREINSVNDNPLIDVSRDVAVHG 360
           NENDPLTKPKQDRYALRTSPQWLGPQIEVIRMATHSIEREINSVNDNPLIDVSRDVAVHG
Sbjct: 337 NENDPLTKPKQDRYALRTSPQWLGPQIEVIRMATHSIEREINSVNDNPLIDVSRDVAVHG 396

Query: 361 GNFQGTPIGVSMDNLRIAIAAIGKLMFAQFSELVCHHYNNGLPSNLSGGPNPSLDYGFKG 420
           GNFQGTPIGVSMDNLRIAIAAIGKLMFAQFSELVCHHYNNGLPSNLSGGPNPSLDYGFKG
Sbjct: 397 GNFQGTPIGVSMDNLRIAIAAIGKLMFAQFSELVCHHYNNGLPSNLSGGPNPSLDYGFKG 456

Query: 421 AEIAMAAYCSELQYLANPVTTHVQSAEQHNQDVNSLGLISARKSAEAIEILKLMSATYMV 480
           AEIAMAAYCSELQYLANPVTTHVQSAEQHNQDVNSLGLISARKSAEAIEILKLMSATYMV
Sbjct: 457 AEIAMAAYCSELQYLANPVTTHVQSAEQHNQDVNSLGLISARKSAEAIEILKLMSATYMV 516

Query: 481 ALCQAIDLRQFEENMREVVKNALLQTIRKTLYMAEYGSLLESRFCEKDLLQVIEREEVFS 540
           ALCQAIDLRQFEENMREVVKNALLQTIRKTLYMAEYGSLLESRFCEKDLLQVIEREEVFS
Sbjct: 517 ALCQAIDLRQFEENMREVVKNALLQTIRKTLYMAEYGSLLESRFCEKDLLQVIEREEVFS 576

Query: 541 YIDDPTNTSYTLIPKLLEVLVEKALKGVATGKTDNGNETGIAIFKQIPEFLEELKAKLEK 600
           YIDDPTNTSYTLIPKLLEVLVEKALKGVATGKTDNGNETGIAIFKQIPEFLEELKAKLEK
Sbjct: 577 YIDDPTNTSYTLIPKLLEVLVEKALKGVATGKTDNGNETGIAIFKQIPEFLEELKAKLEK 636

Query: 601 DVENARGRFENGDFEIENRIKKCSTYPIYRFVRNEVGTQLLSGAKKVSPGEDIEKVVEAI 660
           DVENARGRFENGDFEIENRIKKCSTYPIYRFVRNEVGTQLLSGAKKVSPGEDIEKVVEAI
Sbjct: 637 DVENARGRFENGDFEIENRIKKCSTYPIYRFVRNEVGTQLLSGAKKVSPGEDIEKVVEAI 696

Query: 661 DEGKLGNILMECLKN 676
           DEGKLGNILMECLKN
Sbjct: 697 DEGKLGNILMECLKN 711

BLAST of Cucsa.155520 vs. NCBI nr
Match: gi|659100043|ref|XP_008450900.1| (PREDICTED: phenylalanine ammonia-lyase-like [Cucumis melo])

HSP 1 Score: 1275.4 bits (3299), Expect = 0.0e+00
Identity = 651/675 (96.44%), Postives = 663/675 (98.22%), Query Frame = 1

Query: 1   ANALSQTHFEEVCRMLSEFQEAQTVDVEGTTLTVAQVATIARRSDVKVILNESVAHDRVA 60
           ANALS+THFEEVC MLSEFQEAQTVDVEGT LTVAQV  IARRS+VKVI+NESVAHDRVA
Sbjct: 72  ANALSRTHFEEVCCMLSEFQEAQTVDVEGTKLTVAQVTAIARRSNVKVIMNESVAHDRVA 131

Query: 61  ESAKWVAEKVSSGIDIYGVTTGFGATSHLRTTKISDLQTELIRFLNAGVIGKDNLPSCYS 120
           +SAKWVAEKVSSGIDIYGVTTGFGATSH RTTKISDLQTELIRFLNAGVIGK+NLPS YS
Sbjct: 132 KSAKWVAEKVSSGIDIYGVTTGFGATSHRRTTKISDLQTELIRFLNAGVIGKENLPSSYS 191

Query: 121 KVAMLVRTNTLMQGYSGIRWEILEALVKLMNENLIPKLPLRGTITASGDLVPLSYIAGVV 180
           K AMLVRTNTLMQGYSGIRWEILEA+VKLMN NLIPKLPLRGTITASGDLVPLSYIAGVV
Sbjct: 192 KAAMLVRTNTLMQGYSGIRWEILEAVVKLMNWNLIPKLPLRGTITASGDLVPLSYIAGVV 251

Query: 181 TGRHNSKVTTIEGDEITAIEALKRAGVAGPFELQAKEGLALVNGTAVGSAVAATVCYDAN 240
           TGRHNSKVTTIEGDEI+AIEALKRAG+AGPFELQAKEGLALVNGTAVGSAVAATVCYDAN
Sbjct: 252 TGRHNSKVTTIEGDEISAIEALKRAGIAGPFELQAKEGLALVNGTAVGSAVAATVCYDAN 311

Query: 241 ILALLASVLSALFCEAMLGKPEFTDPLTHKLKHHPGQIESAAVMEFLLNQSDFMKEAKIC 300
           ILALLASVLSALFCEAMLGKPEFTDPLTHKLKHHPGQIESAAVMEFLLNQSDFMKEAKIC
Sbjct: 312 ILALLASVLSALFCEAMLGKPEFTDPLTHKLKHHPGQIESAAVMEFLLNQSDFMKEAKIC 371

Query: 301 NENDPLTKPKQDRYALRTSPQWLGPQIEVIRMATHSIEREINSVNDNPLIDVSRDVAVHG 360
            ENDPLTKPKQDRYALRTSPQWLGPQIEVIRMATHSIEREINSVNDNPLIDVSRDVAVHG
Sbjct: 372 IENDPLTKPKQDRYALRTSPQWLGPQIEVIRMATHSIEREINSVNDNPLIDVSRDVAVHG 431

Query: 361 GNFQGTPIGVSMDNLRIAIAAIGKLMFAQFSELVCHHYNNGLPSNLSGGPNPSLDYGFKG 420
           GNFQGTPIGVSMDNLRIAIAAIGKLMFAQFSELVCHHYNNGLPSNLSGGPNPSLDYGFKG
Sbjct: 432 GNFQGTPIGVSMDNLRIAIAAIGKLMFAQFSELVCHHYNNGLPSNLSGGPNPSLDYGFKG 491

Query: 421 AEIAMAAYCSELQYLANPVTTHVQSAEQHNQDVNSLGLISARKSAEAIEILKLMSATYMV 480
           AEIAMAAYCSELQYLANPVTTHVQSAEQHNQDVNSLGLISARKSAEAIEILKLMSATYMV
Sbjct: 492 AEIAMAAYCSELQYLANPVTTHVQSAEQHNQDVNSLGLISARKSAEAIEILKLMSATYMV 551

Query: 481 ALCQAIDLRQFEENMREVVKNALLQTIRKTLYMAEYGSLLESRFCEKDLLQVIEREEVFS 540
           ALCQAIDLRQ+EENMREVVKNALLQTIRKTLYMAEYGSLLESRFCEKDLLQVIEREEVFS
Sbjct: 552 ALCQAIDLRQYEENMREVVKNALLQTIRKTLYMAEYGSLLESRFCEKDLLQVIEREEVFS 611

Query: 541 YIDDPTNTSYTLIPKLLEVLVEKALKGVATGKTDNGNETGIAIFKQIPEFLEELKAKLEK 600
           YIDDPTNTSYTLIPKLLEVLVEKALKGVAT K DN NETGIAIFKQIPEFLEELKAKLEK
Sbjct: 612 YIDDPTNTSYTLIPKLLEVLVEKALKGVATDKKDNRNETGIAIFKQIPEFLEELKAKLEK 671

Query: 601 DVENARGRFENGDFEIENRIKKCSTYPIYRFVRNEVGTQLLSGAKKVSPGEDIEKVVEAI 660
           DVENARGRFENGDFEIENRIKKCSTYPIYRFVRNE+GTQLLSGAKK+SPGEDIEKVVEAI
Sbjct: 672 DVENARGRFENGDFEIENRIKKCSTYPIYRFVRNELGTQLLSGAKKISPGEDIEKVVEAI 731

Query: 661 DEGKLGNILMECLKN 676
           DEGKLGN+LMECLKN
Sbjct: 732 DEGKLGNVLMECLKN 746

BLAST of Cucsa.155520 vs. NCBI nr
Match: gi|778663077|ref|XP_011660012.1| (PREDICTED: phenylalanine ammonia-lyase isoform X2 [Cucumis sativus])

HSP 1 Score: 1080.1 bits (2792), Expect = 0.0e+00
Identity = 552/552 (100.00%), Postives = 552/552 (100.00%), Query Frame = 1

Query: 1   ANALSQTHFEEVCRMLSEFQEAQTVDVEGTTLTVAQVATIARRSDVKVILNESVAHDRVA 60
           ANALSQTHFEEVCRMLSEFQEAQTVDVEGTTLTVAQVATIARRSDVKVILNESVAHDRVA
Sbjct: 37  ANALSQTHFEEVCRMLSEFQEAQTVDVEGTTLTVAQVATIARRSDVKVILNESVAHDRVA 96

Query: 61  ESAKWVAEKVSSGIDIYGVTTGFGATSHLRTTKISDLQTELIRFLNAGVIGKDNLPSCYS 120
           ESAKWVAEKVSSGIDIYGVTTGFGATSHLRTTKISDLQTELIRFLNAGVIGKDNLPSCYS
Sbjct: 97  ESAKWVAEKVSSGIDIYGVTTGFGATSHLRTTKISDLQTELIRFLNAGVIGKDNLPSCYS 156

Query: 121 KVAMLVRTNTLMQGYSGIRWEILEALVKLMNENLIPKLPLRGTITASGDLVPLSYIAGVV 180
           KVAMLVRTNTLMQGYSGIRWEILEALVKLMNENLIPKLPLRGTITASGDLVPLSYIAGVV
Sbjct: 157 KVAMLVRTNTLMQGYSGIRWEILEALVKLMNENLIPKLPLRGTITASGDLVPLSYIAGVV 216

Query: 181 TGRHNSKVTTIEGDEITAIEALKRAGVAGPFELQAKEGLALVNGTAVGSAVAATVCYDAN 240
           TGRHNSKVTTIEGDEITAIEALKRAGVAGPFELQAKEGLALVNGTAVGSAVAATVCYDAN
Sbjct: 217 TGRHNSKVTTIEGDEITAIEALKRAGVAGPFELQAKEGLALVNGTAVGSAVAATVCYDAN 276

Query: 241 ILALLASVLSALFCEAMLGKPEFTDPLTHKLKHHPGQIESAAVMEFLLNQSDFMKEAKIC 300
           ILALLASVLSALFCEAMLGKPEFTDPLTHKLKHHPGQIESAAVMEFLLNQSDFMKEAKIC
Sbjct: 277 ILALLASVLSALFCEAMLGKPEFTDPLTHKLKHHPGQIESAAVMEFLLNQSDFMKEAKIC 336

Query: 301 NENDPLTKPKQDRYALRTSPQWLGPQIEVIRMATHSIEREINSVNDNPLIDVSRDVAVHG 360
           NENDPLTKPKQDRYALRTSPQWLGPQIEVIRMATHSIEREINSVNDNPLIDVSRDVAVHG
Sbjct: 337 NENDPLTKPKQDRYALRTSPQWLGPQIEVIRMATHSIEREINSVNDNPLIDVSRDVAVHG 396

Query: 361 GNFQGTPIGVSMDNLRIAIAAIGKLMFAQFSELVCHHYNNGLPSNLSGGPNPSLDYGFKG 420
           GNFQGTPIGVSMDNLRIAIAAIGKLMFAQFSELVCHHYNNGLPSNLSGGPNPSLDYGFKG
Sbjct: 397 GNFQGTPIGVSMDNLRIAIAAIGKLMFAQFSELVCHHYNNGLPSNLSGGPNPSLDYGFKG 456

Query: 421 AEIAMAAYCSELQYLANPVTTHVQSAEQHNQDVNSLGLISARKSAEAIEILKLMSATYMV 480
           AEIAMAAYCSELQYLANPVTTHVQSAEQHNQDVNSLGLISARKSAEAIEILKLMSATYMV
Sbjct: 457 AEIAMAAYCSELQYLANPVTTHVQSAEQHNQDVNSLGLISARKSAEAIEILKLMSATYMV 516

Query: 481 ALCQAIDLRQFEENMREVVKNALLQTIRKTLYMAEYGSLLESRFCEKDLLQVIEREEVFS 540
           ALCQAIDLRQFEENMREVVKNALLQTIRKTLYMAEYGSLLESRFCEKDLLQVIEREEVFS
Sbjct: 517 ALCQAIDLRQFEENMREVVKNALLQTIRKTLYMAEYGSLLESRFCEKDLLQVIEREEVFS 576

Query: 541 YIDDPTNTSYTL 553
           YIDDPTNTSYTL
Sbjct: 577 YIDDPTNTSYTL 588

BLAST of Cucsa.155520 vs. NCBI nr
Match: gi|854930815|gb|AKN79307.1| (phenylalanine ammonia-lyase 1 [Betula platyphylla])

HSP 1 Score: 1071.2 bits (2769), Expect = 7.4e-310
Identity = 537/673 (79.79%), Postives = 605/673 (89.90%), Query Frame = 1

Query: 1   ANALSQTHFEEVCRMLSEFQEAQTVDVEGTTLTVAQVATIARRSDVKVILNESVAHDRVA 60
           A AL  THF+EVCRM+S+F + Q+VD++GT+LTVAQV  IARRS+VKV L+E+ A +RVA
Sbjct: 48  AEALQCTHFDEVCRMVSQFAKVQSVDIQGTSLTVAQVTAIARRSEVKVNLDEAAARERVA 107

Query: 61  ESAKWVAEKVSSGIDIYGVTTGFGATSHLRTTKISDLQTELIRFLNAGVIGKDNLPSCYS 120
           +SA WVAE +S G D YGVTTGFGATSH RT K  DLQTELIRFLNAGVIGK++LPS YS
Sbjct: 108 KSANWVAENISRGTDTYGVTTGFGATSHRRTNKTVDLQTELIRFLNAGVIGKESLPSTYS 167

Query: 121 KVAMLVRTNTLMQGYSGIRWEILEALVKLMNENLIPKLPLRGTITASGDLVPLSYIAGVV 180
           KVAMLVRTNTLMQG+SGIRWEILEA+  LMN+NLIPKLPLRGTITASGDLVPLSYIAG++
Sbjct: 168 KVAMLVRTNTLMQGFSGIRWEILEAMANLMNKNLIPKLPLRGTITASGDLVPLSYIAGLL 227

Query: 181 TGRHNSKVTTIEGDEITAIEALKRAGVAGPFELQAKEGLALVNGTAVGSAVAATVCYDAN 240
           TGRHNSKV T E +EITA+EALKRAG+  PFEL+AKEGLALVNGTAVGSAVAATVC+DAN
Sbjct: 228 TGRHNSKVVTPEDEEITALEALKRAGIPAPFELKAKEGLALVNGTAVGSAVAATVCFDAN 287

Query: 241 ILALLASVLSALFCEAMLGKPEFTDPLTHKLKHHPGQIESAAVMEFLLNQSDFMKEAKIC 300
           ILAL A +LSALFCE MLG  EFTDPLTH+LKHHPGQIE+AA+MEFLL++SD+MKEAK+ 
Sbjct: 288 ILALFAEILSALFCEVMLGNAEFTDPLTHELKHHPGQIEAAAIMEFLLDESDYMKEAKLR 347

Query: 301 NENDPLTKPKQDRYALRTSPQWLGPQIEVIRMATHSIEREINSVNDNPLIDVSRDVAVHG 360
            E DPLTKPKQDRYALRTSPQWLGPQIEVIRMATHSIEREINSVNDNPLIDV+RD+A+HG
Sbjct: 348 YEKDPLTKPKQDRYALRTSPQWLGPQIEVIRMATHSIEREINSVNDNPLIDVARDIALHG 407

Query: 361 GNFQGTPIGVSMDNLRIAIAAIGKLMFAQFSELVCHHYNNGLPSNLSGGPNPSLDYGFKG 420
           GNFQGTPIGVSMDNLRIA+AAIGKLMFAQFSELVC +YNNGLPSNLSGGPNPSLDYGFKG
Sbjct: 408 GNFQGTPIGVSMDNLRIAVAAIGKLMFAQFSELVCDYYNNGLPSNLSGGPNPSLDYGFKG 467

Query: 421 AEIAMAAYCSELQYLANPVTTHVQSAEQHNQDVNSLGLISARKSAEAIEILKLMSATYMV 480
           AEIAMA+YCSELQYLANPVTTHVQSAEQHNQDVNSLGLISARKSAEAIEILKLM ATYMV
Sbjct: 468 AEIAMASYCSELQYLANPVTTHVQSAEQHNQDVNSLGLISARKSAEAIEILKLMVATYMV 527

Query: 481 ALCQAIDLRQFEENMREVVKNALLQTIRKTLYMAEYGSLLESRFCEKDLLQVIEREEVFS 540
            LCQAIDLR  EENM+EVVK+ +LQ +RKTLY AE GSLLESRFCEK+LLQVIE + VF+
Sbjct: 528 GLCQAIDLRHLEENMKEVVKHVVLQVVRKTLYTAEDGSLLESRFCEKELLQVIEHQPVFN 587

Query: 541 YIDDPTNTSYTLIPKLLEVLVEKALKGVATGKTDNGNETGIAIFKQIPEFLEELKAKLEK 600
           Y+DDPT++SY L+PKL EVLVEKALK     KT+NG++ G +IFK+IP FL+ELKA+L +
Sbjct: 588 YLDDPTSSSYALLPKLREVLVEKALK--EHPKTENGHDEGFSIFKRIPVFLDELKARLGE 647

Query: 601 DVENARGRFENGDFEIENRIKKCSTYPIYRFVRNEVGTQLLSGAKKVSPGEDIEKVVEAI 660
           +V  AR RFE GDF + NRIKKC TYPIYRFVR EVGT LL+GAKKVSPGEDIEKV EAI
Sbjct: 648 EVVKARDRFEKGDFPMPNRIKKCRTYPIYRFVRTEVGTTLLTGAKKVSPGEDIEKVYEAI 707

Query: 661 DEGKLGNILMECL 674
           + GKLG+++++CL
Sbjct: 708 NGGKLGDVVVKCL 718

BLAST of Cucsa.155520 vs. NCBI nr
Match: gi|1009143501|ref|XP_015889298.1| (PREDICTED: phenylalanine ammonia-lyase-like [Ziziphus jujuba])

HSP 1 Score: 1065.4 bits (2754), Expect = 3.9e-308
Identity = 539/676 (79.73%), Postives = 601/676 (88.91%), Query Frame = 1

Query: 1   ANALSQTHFEEVCRMLSEFQEAQTVDVEGTTLTVAQVATIARRSDVKVILNESVAHDRVA 60
           A AL   HF+EVC +LS+F +A+TVD++GTTLTVAQVA IARRS VKV L+E+ A +RVA
Sbjct: 28  AEALQCNHFDEVCHLLSQFSQAETVDIQGTTLTVAQVAAIARRSQVKVSLDEAEARERVA 87

Query: 61  ESAKWVAEKVSSGIDIYGVTTGFGATSHLRTTKISDLQTELIRFLNAGVIGKDNLPSCYS 120
           +SA WVAE +  G D YGVTTGFGATSH RT K SDLQTELIRFLNAGVIGKDNLP+ YS
Sbjct: 88  KSANWVAENICRGTDTYGVTTGFGATSHRRTNKTSDLQTELIRFLNAGVIGKDNLPTSYS 147

Query: 121 KVAMLVRTNTLMQGYSGIRWEILEALVKLMNENLIPKLPLRGTITASGDLVPLSYIAGVV 180
           K AMLVRTNTLMQG+SGIRWEILEA+VKLMNENLIPKLPLRGTITASGDLVPLSYIAG++
Sbjct: 148 KAAMLVRTNTLMQGFSGIRWEILEAMVKLMNENLIPKLPLRGTITASGDLVPLSYIAGLI 207

Query: 181 TGRHNSKVTTIEGDEITAIEALKRAGVAGPFELQAKEGLALVNGTAVGSAVAATVCYDAN 240
           TGRHNSKV T  G+EITA+EALKRAG+A PFELQAKEGLALVNGTAVGSAVAATVC+DAN
Sbjct: 208 TGRHNSKVITPNGEEITAMEALKRAGIANPFELQAKEGLALVNGTAVGSAVAATVCFDAN 267

Query: 241 ILALLASVLSALFCEAMLGKPEFTDPLTHKLKHHPGQIESAAVMEFLLNQSDFMKEAKIC 300
            LALLA +LSALFCE M GKPEFTDPLTH+LKHHPGQIESAA+M+FLL+ SD+MKEAK+ 
Sbjct: 268 TLALLAEILSALFCEVMHGKPEFTDPLTHELKHHPGQIESAAIMKFLLDGSDYMKEAKLR 327

Query: 301 NENDPLTKPKQDRYALRTSPQWLGPQIEVIRMATHSIEREINSVNDNPLIDVSRDVAVHG 360
           ++ DPLTKPKQDRYA+RTSPQWLGPQIEVIR ATHSIEREINSVNDNPLIDVSRD A+HG
Sbjct: 328 HKKDPLTKPKQDRYAIRTSPQWLGPQIEVIRAATHSIEREINSVNDNPLIDVSRDRALHG 387

Query: 361 GNFQGTPIGVSMDNLRIAIAAIGKLMFAQFSELVCHHYNNGLPSNLSGGPNPSLDYGFKG 420
           GNFQGTPIGVSMDN+RIAIAAIGKLMFAQFSELVC +YNNGLPSNLSGGPNPSLDYG KG
Sbjct: 388 GNFQGTPIGVSMDNIRIAIAAIGKLMFAQFSELVCDYYNNGLPSNLSGGPNPSLDYGLKG 447

Query: 421 AEIAMAAYCSELQYLANPVTTHVQSAEQHNQDVNSLGLISARKSAEAIEILKLMSATYMV 480
           AEIAMAAYCSELQYLANPVTTHVQSAEQHNQDVNSLGLISARKSAEAIEILKLMSATYMV
Sbjct: 448 AEIAMAAYCSELQYLANPVTTHVQSAEQHNQDVNSLGLISARKSAEAIEILKLMSATYMV 507

Query: 481 ALCQAIDLRQFEENMREVVKNALLQTIRKTLYMAEYGSLLESRFCEKDLLQVIEREEVFS 540
            LCQAIDLR  EENM+EVVK  LLQ +RKTLY AE GSLLESRFCEK+LLQV E + VFS
Sbjct: 508 GLCQAIDLRHLEENMKEVVKVVLLQVVRKTLYSAEDGSLLESRFCEKELLQVAEHQPVFS 567

Query: 541 YIDDPTNTSYTLIPKLLEVLVEKALKGVATGKTDNGNETGIAIFKQIPEFLEELKAKLEK 600
           Y+DDPTN +Y L+PKL EV+VE+ALKG    K ++ +    +IFK+IP F+EEL+A+L  
Sbjct: 568 YLDDPTNPAYALLPKLREVIVERALKG---HKAEDADGRNYSIFKRIPIFMEELEARLGA 627

Query: 601 DVENARGRFENGDFE-IENRIKKCSTYPIYRFVRNEVGTQLLSGAKKVSPGEDIEKVVEA 660
           +V  AR RF+NGD+  IENRI KC TYPIY+FVR+EVGT+LLSGAKK+SPGEDIEKV EA
Sbjct: 628 EVAKARERFDNGDYRPIENRINKCRTYPIYKFVRSEVGTELLSGAKKISPGEDIEKVYEA 687

Query: 661 IDEGKLGNILMECLKN 676
           I+E KLG++L  CLK+
Sbjct: 688 INEEKLGDVLKMCLKD 700

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
PALY_PINTA9.0e-24463.61Phenylalanine ammonia-lyase OS=Pinus taeda GN=PAL PE=3 SV=1[more]
PALY_CAMSI3.4e-23563.58Phenylalanine ammonia-lyase OS=Camellia sinensis GN=PAL PE=2 SV=1[more]
PAL2_ARATH7.6e-23562.85Phenylalanine ammonia-lyase 2 OS=Arabidopsis thaliana GN=PAL2 PE=1 SV=2[more]
PAL1_DAUCA1.3e-23463.73Phenylalanine ammonia-lyase 1 OS=Daucus carota GN=PAL1 PE=3 SV=1[more]
PAL2_ORYSI1.1e-23363.17Phenylalanine ammonia-lyase OS=Oryza sativa subsp. indica GN=ZB8 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
A0A0A0LYZ4_CUCSA0.0e+00100.00Phenylalanine ammonia-lyase OS=Cucumis sativus GN=Csa_1G590300 PE=3 SV=1[more]
A0A0H4CUQ9_BETPL5.1e-31079.79Phenylalanine ammonia-lyase OS=Betula platyphylla GN=PAL1 PE=2 SV=1[more]
A0A067KCD4_JATCU1.8e-30778.52Phenylalanine ammonia-lyase OS=Jatropha curcas GN=JCGZ_07452 PE=3 SV=1[more]
A0A059CW46_EUCGR9.8e-30677.93Phenylalanine ammonia-lyase OS=Eucalyptus grandis GN=EUGRSUZ_C03570 PE=3 SV=1[more]
E7BLC8_BETPL1.7e-30579.11Phenylalanine ammonia-lyase OS=Betula platyphylla GN=PAL PE=2 SV=1[more]
Match NameE-valueIdentityDescription
AT3G53260.14.3e-23662.85 phenylalanine ammonia-lyase 2[more]
AT3G10340.16.8e-23462.06 phenylalanine ammonia-lyase 4[more]
AT2G37040.16.8e-23462.65 PHE ammonia lyase 1[more]
AT5G04230.22.1e-21458.31 phenyl alanine ammonia-lyase 3[more]
Match NameE-valueIdentityDescription
gi|449452542|ref|XP_004144018.1|0.0e+00100.00PREDICTED: phenylalanine ammonia-lyase isoform X1 [Cucumis sativus][more]
gi|659100043|ref|XP_008450900.1|0.0e+0096.44PREDICTED: phenylalanine ammonia-lyase-like [Cucumis melo][more]
gi|778663077|ref|XP_011660012.1|0.0e+00100.00PREDICTED: phenylalanine ammonia-lyase isoform X2 [Cucumis sativus][more]
gi|854930815|gb|AKN79307.1|7.4e-31079.79phenylalanine ammonia-lyase 1 [Betula platyphylla][more]
gi|1009143501|ref|XP_015889298.1|3.9e-30879.73PREDICTED: phenylalanine ammonia-lyase-like [Ziziphus jujuba][more]
The following terms have been associated with this gene:
Vocabulary: INTERPRO
TermDefinition
IPR001106Aromatic_Lyase
IPR005922Phe_NH3-lyase
IPR008948L-Aspartase-like
IPR022313Phe/His_NH3-lyase_AS
IPR023144Phe_NH3-lyase_shielding_dom_sf
IPR024083Fumarase/histidase_N
Vocabulary: Cellular Component
TermDefinition
GO:0005737cytoplasm
Vocabulary: Biological Process
TermDefinition
GO:0006559L-phenylalanine catabolic process
Vocabulary: Molecular Function
TermDefinition
GO:0016841ammonia-lyase activity
GO:0003824catalytic activity
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0009821 alkaloid biosynthetic process
biological_process GO:0009800 cinnamic acid biosynthetic process
biological_process GO:0006559 L-phenylalanine catabolic process
biological_process GO:0006570 tyrosine metabolic process
cellular_component GO:0005737 cytoplasm
molecular_function GO:0045548 phenylalanine ammonia-lyase activity
molecular_function GO:0016841 ammonia-lyase activity
molecular_function GO:0003824 catalytic activity

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Cucsa.155520.1Cucsa.155520.1mRNA


Analysis Name: InterPro Annotations of cucumber (Gy14)
Date Performed: 2017-01-17
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001106Aromatic amino acid lyasePFAMPF00221Lyase_aromaticcoord: 32..508
score: 6.5E
IPR005922Phenylalanine ammonia-lyaseTIGRFAMsTIGR01226TIGR01226coord: 2..674
score: 1.7E
IPR008948L-Aspartase-likeunknownSSF48557L-aspartase-likecoord: 5..674
score: 6.83E
IPR022313Phenylalanine/histidine ammonia-lyases, active sitePROSITEPS00488PAL_HISTIDASEcoord: 162..178
scor
IPR023144Phenylalanine ammonia-lyase, shielding domainGENE3DG3DSA:1.10.274.20coord: 498..618
score: 7.2
IPR024083Fumarase/histidase, N-terminalGENE3DG3DSA:1.10.275.10coord: 2..224
score: 5.5
NoneNo IPR availableGENE3DG3DSA:1.20.200.10coord: 225..497
score: 1.2E-136coord: 619..673
score: 1.2E
NoneNo IPR availablePANTHERPTHR10362HISTIDINE AMMONIA-LYASEcoord: 1..673
score:
NoneNo IPR availablePANTHERPTHR10362:SF18SUBFAMILY NOT NAMEDcoord: 1..673
score: