Cucsa.146880 (gene) Cucumber (Gy14) v1

BLAST of Cucsa.146880 vs. Swiss-Prot
Match: BGH3B_BACO1 (Beta-glucosidase BoGH3B OS=Bacteroides ovatus (strain ATCC 8483 / DSM 1896 / JCM 5824 / NCTC 11153) GN=BACOVA_02659 PE=1 SV=1)

HSP 1 Score: 302.0 bits (772), Expect = 1.5e-80
Identity = 209/645 (32.40%), Postives = 328/645 (50.85%), Query Frame = 1

Query: 28  AIEDRIKDLLSRMSLREKIGQMTQIERSVVTP------------SALTDLAVGSVLSGGD 87
           AIE  I++ L +M+L +KIGQM +I   VV+              A+ D  +G    G  
Sbjct: 34  AIETHIREWLQKMTLEQKIGQMCEITIDVVSDLETSRKKGFCLSEAMLDTVIGKYKVGSL 93

Query: 88  -NPPFDKAMSLD-WADMVDGFQSLALQSRLGIPIIYGIDAVHGSSNVYGATIFPHNVGLG 147
            N P   A   + WA+ +   Q  +++  +GIP IYG+D +HG++     T+FP  + +G
Sbjct: 94  LNVPLGVAQKKEKWAEAIKQIQEKSMKE-IGIPCIYGVDQIHGTTYTLDGTMFPQGINMG 153

Query: 148 ATRDGKLVRRIGTVTALEVRASGVHYAFAPCLAVSRDPRWGRCYESYSEHTEVVRKM-TS 207
           AT + +L RR   ++A E +A  + + FAP + + RDPRW R +E+Y E   V  +M  S
Sbjct: 154 ATFNRELTRRGAKISAYETKAGCIPWTFAPVVDLGRDPRWARMWENYGEDCYVNAEMGVS 213

Query: 208 LVEGLQGKPPEGYPKGYPFVAGRNNVIACAKHFVGDGGTDKGLNEGNTIIDSYDELERIH 267
            V+G QG+ P           G  NV AC KH++G G    G +   + I   D  E+ H
Sbjct: 214 AVKGFQGEDPNR--------IGEYNVAACMKHYMGYGVPVSGKDRTPSSISRSDMREK-H 273

Query: 268 IAPYLDCIAQGLSTVMASYSSWNGNPLHTHHFLLTQVLKEKLGFKGFVISDWEALDRL-- 327
            AP+L  + QG  +VM +    NG P H +  LLT+ LKE L + G +++DW  ++ L  
Sbjct: 274 FAPFLAAVRQGALSVMVNSGVDNGLPFHANRELLTEWLKEDLNWDGLIVTDWADINNLCT 333

Query: 328 SNPRGSNYRSCICTAVNAGIDMVMVPFRYEEFIKDLLSLVESGEIPIARIDDAVERILRV 387
            +   +  +  +   +NAGIDM MVP+    F   L  LVE GE+ + RIDDAV R+LR+
Sbjct: 334 RDHIAATKKEAVKIVINAGIDMSMVPYEV-SFCDYLKELVEEGEVSMERIDDAVARVLRL 393

Query: 388 KFVAGLFEHPFSDRSLIDVVGCKIHRDLAREAVRKSLVLLRNGKDPMKPFLPLDRKAKKI 447
           K+  GLF+HP+ D    D  G K    +A +A  +S VLL+N  +     LP+  K KKI
Sbjct: 394 KYRLGLFDHPYWDIKKYDKFGSKEFAAVALQAAEESEVLLKNDGN----ILPI-AKGKKI 453

Query: 448 LVAGSHADDLGYQCGGWTISWNGSTGRTTVGT--TILDAIKEAVGDQTKVIYEQNPSAVT 507
           L+ G +A+ +    GGW+ SW G           TI +A+ E  G +  +IYE   +  +
Sbjct: 454 LLTGPNANSMRCLNGGWSYSWQGHVADEYAQAYHTIYEALCEKYGKE-NIIYEPGVTYAS 513

Query: 508 LNDQD------------------ISFAIVAIGESPYAESAGDNSKLIIPFNGNEIVKAVA 567
             + +                      I  IGE+ Y E+ G+ + L +  N   +VKA+A
Sbjct: 514 YKNDNWWEENKPETEKPVAAAAQADIIITCIGENSYCETPGNLTDLTLSENQRNLVKALA 573

Query: 568 --GKIPTLVILISGRPLVLEPTVIENVEALIAAWLPGT-EGNGITDVIFGDYDFTGRLPV 618
             GK P +++L  GRP ++   ++   +A++   LP    G+ + +++ GD +F+G++P 
Sbjct: 574 ATGK-PIVLVLNQGRPRIIN-DIVPLAKAVVNIMLPSNYGGDALANLLAGDANFSGKMPF 633

BLAST of Cucsa.146880 vs. Swiss-Prot
Match: GLUA_DICDI (Lysosomal beta glucosidase OS=Dictyostelium discoideum GN=gluA PE=1 SV=2)

HSP 1 Score: 272.7 bits (696), Expect = 9.9e-72
Identity = 200/632 (31.65%), Postives = 327/632 (51.74%), Query Frame = 1

Query: 33  IKDLLSRMSLREKIGQMTQIERSVVT-PSALT----DLA-------VGSVL----SGGDN 92
           + +L+S+MS+ EKIGQMTQ++ + +T P+ +T     LA       +GS L    SGG  
Sbjct: 80  VDNLMSKMSITEKIGQMTQLDITTLTSPNTITINETTLAYYAKTYYIGSYLNSPVSGGLA 139

Query: 93  PPFDKAMSLDWADMVDGFQSLALQ-SRLGIPIIYGIDAVHGSSNVYGATIFPHNVGLGAT 152
                  S  W DM++  Q++ ++ S   IP+IYG+D+VHG++ V+ AT+FPHN GL AT
Sbjct: 140 GDIHHINSSVWLDMINTIQTIVIEGSPNKIPMIYGLDSVHGANYVHKATLFPHNTGLAAT 199

Query: 153 RDGKLVRRIGTVTALEVRASGVHYAFAPCLAVSRDPRWGRCYESYSEHTEVVRKM-TSLV 212
            + +       +T+ +  A G+ + FAP L +   P W R YE++ E   V   M  + V
Sbjct: 200 FNIEHATTAAQITSKDTVAVGIPWVFAPVLGIGVQPLWSRIYETFGEDPYVASMMGAAAV 259

Query: 213 EGLQGKPPEGYPKGYPFVAGRNNVIACAKHFVGDGGTDKGLNEGNTIIDSYDELERIHIA 272
            G QG         +       + +  AKH+ G      G +     I     L R  + 
Sbjct: 260 RGFQGG-----NNSFDGPINAPSAVCTAKHYFGYSDPTSGKDRTAAWIPE-RMLRRYFLP 319

Query: 273 PYLDCI-AQGLSTVMASYSSWNGNPLHTHHFLLTQVLKEKLGFKGFVISDWEALDRL--S 332
            + + I   G  T+M +    NG P+HT +  LT+VL+ +L F+G  ++DW+ +++L   
Sbjct: 320 SFAEAITGAGAGTIMINSGEVNGVPMHTSYKYLTEVLRGELQFEGVAVTDWQDIEKLVYF 379

Query: 333 NPRGSNYRSCICTAVNAGIDMVMVPFRYEEFIKDLLSLVESGEIPIARIDDAVERILRVK 392
           +    +    I  A++AGIDM MVP     F   L  +V +G +P +R+D +V RIL +K
Sbjct: 380 HHTAGSAEEAILQALDAGIDMSMVPLDL-SFPIILAEMVAAGTVPESRLDLSVRRILNLK 439

Query: 393 FVAGLFEHPF--SDRSLIDVVGCKIHRDLAREAVRKSLVLLRNGKDPMKPFLPLDRKA-K 452
           +  GLF +P+   + +++D +G    R+ A     +S+ LL+N  +     LPL+    K
Sbjct: 440 YALGLFSNPYPNPNAAIVDTIGQVQDREAAAATAEESITLLQNKNN----ILPLNTNTIK 499

Query: 453 KILVAGSHADDLGYQCGGWTISWNGS--TGRTTVGTTILDAIKEAVGD------QTKVIY 512
            +L+ G  AD +    GGW++ W G+        GT+IL  ++E   D      Q  + +
Sbjct: 500 NVLLTGPSADSIRNLNGGWSVHWQGAYEDSEFPFGTSILTGLREITNDTADFNIQYTIGH 559

Query: 513 EQN-PSAVTLNDQDISFA------IVAIGESPYAESAGDNSKLIIPFNGNEIVKAV--AG 572
           E   P+  T  D+ +  A      +V IGE P AE+ GD   L +  N   +++ +   G
Sbjct: 560 EIGVPTNQTSIDEAVELAQSSDVVVVVIGELPEAETPGDIYDLSMDPNEVLLLQQLVDTG 619

Query: 573 KIPTLVILISGRPLVLEPTVIENVEALIAAWLPGTE-GNGITDVIFGDYDFTGRLPVTWF 618
           K P ++IL+  RP +L P ++ +  A++ A+LPG+E G  I +++ G+ + +GRLP+T+ 
Sbjct: 620 K-PVVLILVEARPRILPPDLVYSCAAVLMAYLPGSEGGKPIANILMGNVNPSGRLPLTYP 679

BLAST of Cucsa.146880 vs. Swiss-Prot
Match: BGLX_SALTY (Periplasmic beta-glucosidase OS=Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720) GN=bglX PE=3 SV=2)

HSP 1 Score: 257.7 bits (657), Expect = 3.3e-67
Identity = 198/648 (30.56%), Postives = 315/648 (48.61%), Query Frame = 1

Query: 33  IKDLLSRMSLREKIGQMTQIERSVVTPSALTDLAVGSVLSGGDNPPFDKAMSLDWADMVD 92
           + DLL +M++ EKIGQ+  I    V P    +     +  G     F+     D   M D
Sbjct: 38  VTDLLKKMTVDEKIGQLRLIS---VGPDNPKEAIREMIKDGQVGAIFNTVTRQDIRQMQD 97

Query: 93  GFQSLALQSRLGIPIIYGIDAVHGSSNVYGATIFPHNVGLGATRDGKLVRRIGTVTALEV 152
              +L   SRL IP+ +  D VHG       T+FP ++GL ++ +   VR +G V+A E 
Sbjct: 98  QVMAL---SRLKIPLFFAYDVVHGQR-----TVFPISLGLASSFNLDAVRTVGRVSAYEA 157

Query: 153 RASGVHYAFAPCLAVSRDPRWGRCYESYSEHTEVVRKM-TSLVEGLQGKPPEGYPKGYPF 212
              G++  +AP + VSRDPRWGR  E + E T +   M  ++V+ +QGK P         
Sbjct: 158 ADDGLNMTWAPMVDVSRDPRWGRASEGFGEDTYLTSIMGETMVKAMQGKSP--------- 217

Query: 213 VAGRNNVIACAKHFVGDGGTDKGLNEGNTIIDSYDELERIHIAPYLDCIAQGLSTVMASY 272
            A R +V+   KHF   G  + G  E NT+  S   L   ++ PY   +  G   VM + 
Sbjct: 218 -ADRYSVMTSVKHFAAYGAVEGG-KEYNTVDMSSQRLFNDYMPPYKAGLDAGSGAVMVAL 277

Query: 273 SSWNGNPLHTHHFLLTQVLKEKLGFKGFVISDWEALDRL-SNPRGSNYRSCICTAVNAGI 332
           +S NG P  +  +LL  VL+++ GFKG  +SD  A+  L  +   ++    +  A+ AG+
Sbjct: 278 NSLNGTPATSDSWLLKDVLRDEWGFKGITVSDHGAIKELIKHGTAADPEDAVRVALKAGV 337

Query: 333 DMVMVPFRYEEFIKDLLSLVESGEIPIARIDDAVERILRVKFVAGLFEHPFS-----DRS 392
           DM M    Y +++     L++SG++ +A +DDA   +L VK+  GLF  P+S     +  
Sbjct: 338 DMSMADEYYSKYLP---GLIKSGKVTMAELDDATRHVLNVKYDMGLFNDPYSHLGPKESD 397

Query: 393 LIDV-VGCKIHRDLAREAVRKSLVLLRNGKDPMKPFLPLDRKAKKILVAGSHADDLGYQC 452
            +D     ++HR  ARE  R+S+VLL+N  +     LPL +K+  I V G  AD      
Sbjct: 398 PVDTNAESRLHRKEAREVARESVVLLKNRLET----LPL-KKSGTIAVVGPLADSQRDVM 457

Query: 453 GGWTISWNGSTGRTTVGTTILDAIKEAVGDQTKVIYEQNPS----------------AVT 512
           G W+     + G      T+L  I+ AVGD  K++Y +  +                AV 
Sbjct: 458 GSWS-----AAGVANQSVTVLAGIQNAVGDGAKILYAKGANITNDKGIVDFLNLYEEAVK 517

Query: 513 LN--------DQDISFA------IVAIGESP-YAESAGDNSKLIIPFNGNEIVKAV--AG 572
           ++        D+ +  A      +  +GES   A  A   + + IP +  +++ A+   G
Sbjct: 518 IDPRSPQAMIDEAVQAAKQADVVVAVVGESQGMAHEASSRTNITIPQSQRDLITALKATG 577

Query: 573 KIPTLVILISGRPLVLEPTVIENVEALIAAWLPGTEG-NGITDVIFGDYDFTGRLPVTWF 618
           K P +++L++GRPL L     +  +A++  W  GTEG N I DV+FGDY+ +G+LP+++ 
Sbjct: 578 K-PLVLVLMNGRPLALVKED-QQADAILETWFAGTEGGNAIADVLFGDYNPSGKLPISFP 637

BLAST of Cucsa.146880 vs. Swiss-Prot
Match: BGLX_ECOLI (Periplasmic beta-glucosidase OS=Escherichia coli (strain K12) GN=bglX PE=3 SV=2)

HSP 1 Score: 252.3 bits (643), Expect = 1.4e-65
Identity = 194/649 (29.89%), Postives = 309/649 (47.61%), Query Frame = 1

Query: 33  IKDLLSRMSLREKIGQMTQIERSVVTPSALTDLAVGSVLSGGDNPPFDKAMSLDWADMVD 92
           + +LL +M++ EKIGQ+  I    V P    +     +  G     F+     D   M D
Sbjct: 38  VTELLKKMTVDEKIGQLRLIS---VGPDNPKEAIREMIKDGQVGAIFNTVTRQDIRAMQD 97

Query: 93  GFQSLALQSRLGIPIIYGIDAVHGSSNVYGATIFPHNVGLGATRDGKLVRRIGTVTALEV 152
               L   SRL IP+ +  D +HG       T+FP ++GL ++ +   V+ +G V+A E 
Sbjct: 98  QVMEL---SRLKIPLFFAYDVLHGQR-----TVFPISLGLASSFNLDAVKTVGRVSAYEA 157

Query: 153 RASGVHYAFAPCLAVSRDPRWGRCYESYSEHTEVVRKM-TSLVEGLQGKPPEGYPKGYPF 212
              G++  +AP + VSRDPRWGR  E + E T +   M  ++VE +QGK P         
Sbjct: 158 ADDGLNMTWAPMVDVSRDPRWGRASEGFGEDTYLTSTMGKTMVEAMQGKSP--------- 217

Query: 213 VAGRNNVIACAKHFVGDGGTDKGLNEGNTIIDSYDELERIHIAPYLDCIAQGLSTVMASY 272
            A R +V+   KHF   G  + G  E NT+  S   L   ++ PY   +  G   VM + 
Sbjct: 218 -ADRYSVMTSVKHFAAYGAVEGG-KEYNTVDMSPQRLFNDYMPPYKAGLDAGSGAVMVAL 277

Query: 273 SSWNGNPLHTHHFLLTQVLKEKLGFKGFVISDWEALDRL-SNPRGSNYRSCICTAVNAGI 332
           +S NG P  +  +LL  VL+++ GFKG  +SD  A+  L  +   ++    +  A+ +GI
Sbjct: 278 NSLNGTPATSDSWLLKDVLRDQWGFKGITVSDHGAIKELIKHGTAADPEDAVRVALKSGI 337

Query: 333 DMVMVPFRYEEFIKDLLSLVESGEIPIARIDDAVERILRVKFVAGLFEHPFS-----DRS 392
           +M M    Y +++     L++SG++ +A +DDA   +L VK+  GLF  P+S     +  
Sbjct: 338 NMSMSDEYYSKYLP---GLIKSGKVTMAELDDAARHVLNVKYDMGLFNDPYSHLGPKESD 397

Query: 393 LIDV-VGCKIHRDLAREAVRKSLVLLRNGKDPMKPFLPLDRKAKKILVAGSHADDLGYQC 452
            +D     ++HR  ARE  R+SLVLL+N  +     LPL +K+  I V G  AD      
Sbjct: 398 PVDTNAESRLHRKEAREVARESLVLLKNRLET----LPL-KKSATIAVVGPLADSKRDVM 457

Query: 453 GGWTISWNGSTGRTTVGTTILDAIKEAVGDQTKVIYEQNPSAV----------------- 512
           G W+     + G      T+L  IK AVG+  KV+Y +  +                   
Sbjct: 458 GSWS-----AAGVADQSVTVLTGIKNAVGENGKVLYAKGANVTSDKGIIDFLNQYEEAVK 517

Query: 513 ---------------TLNDQDISFAIVAIGESPYAESAGDNSKLIIPFNGNEIVKAV--A 572
                          T    D+  A+V   +   A  A   + + IP +  +++ A+   
Sbjct: 518 VDPRSPQEMIDEAVQTAKQSDVVVAVVGEAQG-MAHEASSRTDITIPQSQRDLIAALKAT 577

Query: 573 GKIPTLVILISGRPLVLEPTVIENVEALIAAWLPGTEG-NGITDVIFGDYDFTGRLPVTW 618
           GK P +++L++GRPL L     +  +A++  W  GTEG N I DV+FGDY+ +G+LP+++
Sbjct: 578 GK-PLVLVLMNGRPLALVKED-QQADAILETWFAGTEGGNAIADVLFGDYNPSGKLPMSF 637

BLAST of Cucsa.146880 vs. Swiss-Prot
Match: BGLC_ASPFN (Probable beta-glucosidase C OS=Aspergillus flavus (strain ATCC 200026 / FGSC A1120 / NRRL 3357 / JCM 12722 / SRRC 167) GN=bglC PE=3 SV=1)

HSP 1 Score: 183.7 bits (465), Expect = 6.1e-45
Identity = 180/650 (27.69%), Postives = 281/650 (43.23%), Query Frame = 1

Query: 21  MYRNPGAAIEDRIKDLLSRMSLREKIGQMTQI---------ERSVVTPSALTDLAVGSVL 80
           +YR+    I++R+ DLL+RM++ EK GQ+            E S       T   +G   
Sbjct: 41  IYRDASYCIDERVDDLLARMTIEEKAGQLFHTRLMDGPLDDEGSGNNAHNSTSNMIGEKH 100

Query: 81  SGGDNPPFDKAMSLDWADMVDGFQSLALQSRLGIPIIYGIDAVHGSSNVYG----ATIF- 140
               N   D   + + A+ ++  Q LALQ+RLGIP+    D  H  +   G    A +F 
Sbjct: 101 MTHFNLASDITNATETAEFINRIQELALQTRLGIPVTVSTDPRHSFTENVGTGFKAGVFS 160

Query: 141 --PHNVGLGATRDGKLVRRIGTVTALEVRASGVHYAFAPCLAVSRDPRWGRCYESYSEHT 200
             P ++GL A RD  +VR+   V   E  A G+  A  P + +S +PRW R   ++ E++
Sbjct: 161 QWPESIGLAALRDPYVVRKFAEVAKEEYIAVGIRAALHPQVDLSTEPRWARISNTWGENS 220

Query: 201 EVVRKM-TSLVEGLQGKPPEGYPKGYPFVAGRNNVIACAKHFVGDG----GTDKGLNEGN 260
            +  ++    ++G QG              G  +V    KHF G G    G D     G 
Sbjct: 221 TLTSELLVEYIKGFQGDK-----------LGPQSVKTVTKHFPGGGPVENGEDSHFAYGK 280

Query: 261 TIIDSYDELERIHIAPYLDCIAQGLSTVMASYSSWNGNPLHTHHF-----LLTQVLKEKL 320
                 + LE  H+ P+   IA G + +M  YS   G       F     ++T++L+ +L
Sbjct: 281 NQTYPGNNLEE-HLKPFKAAIAAGATEIMPYYSRPIGTEYEPVAFSFNKRIVTELLRNEL 340

Query: 321 GFKGFVISDWEALD-----------RLSNPRGSNYRSCICTAVNAGIDMVMVPFRYEEFI 380
           GF+G V++DW  +            R                ++AG D     F  EE  
Sbjct: 341 GFEGIVLTDWGLITDGYIAGQYMPARAWGVENLTELERAARILDAGCDQ----FGGEERP 400

Query: 381 KDLLSLVESGEIPIARIDDAVERILRVKFVAGLFEHPFSD-RSLIDVVGCKIHRDLAREA 440
           + ++ LV+ G +   RID +V R+LR KFV GLF++PF D  S   VVG      L REA
Sbjct: 401 ELIVQLVQEGTVSEDRIDVSVRRLLREKFVLGLFDNPFVDPESAGRVVGNDYFVRLGREA 460

Query: 441 VRKSLVLLRNGKD--PMKPFLPLDRKAKKILVAGSHADDLGYQCGGWTISWNGSTGRTTV 500
            R+S  LL N +D  P+K       ++ K  + G +A         +  SWN        
Sbjct: 461 QRRSYTLLSNNEDIVPLKKI----EQSTKFYIEGFNA--------SFIESWN-------- 520

Query: 501 GTTILDAIKEA--VGDQTKVIYEQNPSAVTLNDQDISFAIVAIGESPYAESAGDNSKLII 560
             T++D+ +EA     +    YE  P     N    S A                     
Sbjct: 521 -YTVVDSPEEADYALLRYNAPYEPRPGGFEANMHAGSLA--------------------- 580

Query: 561 PFNGNEIVK--AVAGKIPTLVILISGRPLVLEPTVIENVEALIAAWLPGTEGNGITDVIF 620
            FN  E  +   +   +PT+V ++  RP V+ P +IE  +A+ A++  G++ N   DV+F
Sbjct: 581 -FNDTEKARQAKIYSTVPTIVDIVMDRPAVI-PEIIEQAKAVFASY--GSDSNAFLDVVF 628

Query: 621 GDYDFTGRLPVTWFKTVEQLPVHAEN---NLQDSLFPFGFGLSYGKEISS 624
           G     G+LP     ++E +    E+   + ++ +F FG GLSY    +S
Sbjct: 641 GVSAPEGKLPFDLPSSMEAVEAQMEDVPFDTRNPVFKFGHGLSYANPCAS 628

BLAST of Cucsa.146880 vs. TrEMBL
Match: A0A0A0LXK2_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_1G528540 PE=3 SV=1)

HSP 1 Score: 1229.9 bits (3181), Expect = 0.0e+00
Identity = 611/611 (100.00%), Postives = 611/611 (100.00%), Query Frame = 1

Query: 14  MESKNDCMYRNPGAAIEDRIKDLLSRMSLREKIGQMTQIERSVVTPSALTDLAVGSVLSG 73
           MESKNDCMYRNPGAAIEDRIKDLLSRMSLREKIGQMTQIERSVVTPSALTDLAVGSVLSG
Sbjct: 1   MESKNDCMYRNPGAAIEDRIKDLLSRMSLREKIGQMTQIERSVVTPSALTDLAVGSVLSG 60

Query: 74  GDNPPFDKAMSLDWADMVDGFQSLALQSRLGIPIIYGIDAVHGSSNVYGATIFPHNVGLG 133
           GDNPPFDKAMSLDWADMVDGFQSLALQSRLGIPIIYGIDAVHGSSNVYGATIFPHNVGLG
Sbjct: 61  GDNPPFDKAMSLDWADMVDGFQSLALQSRLGIPIIYGIDAVHGSSNVYGATIFPHNVGLG 120

Query: 134 ATRDGKLVRRIGTVTALEVRASGVHYAFAPCLAVSRDPRWGRCYESYSEHTEVVRKMTSL 193
           ATRDGKLVRRIGTVTALEVRASGVHYAFAPCLAVSRDPRWGRCYESYSEHTEVVRKMTSL
Sbjct: 121 ATRDGKLVRRIGTVTALEVRASGVHYAFAPCLAVSRDPRWGRCYESYSEHTEVVRKMTSL 180

Query: 194 VEGLQGKPPEGYPKGYPFVAGRNNVIACAKHFVGDGGTDKGLNEGNTIIDSYDELERIHI 253
           VEGLQGKPPEGYPKGYPFVAGRNNVIACAKHFVGDGGTDKGLNEGNTIIDSYDELERIHI
Sbjct: 181 VEGLQGKPPEGYPKGYPFVAGRNNVIACAKHFVGDGGTDKGLNEGNTIIDSYDELERIHI 240

Query: 254 APYLDCIAQGLSTVMASYSSWNGNPLHTHHFLLTQVLKEKLGFKGFVISDWEALDRLSNP 313
           APYLDCIAQGLSTVMASYSSWNGNPLHTHHFLLTQVLKEKLGFKGFVISDWEALDRLSNP
Sbjct: 241 APYLDCIAQGLSTVMASYSSWNGNPLHTHHFLLTQVLKEKLGFKGFVISDWEALDRLSNP 300

Query: 314 RGSNYRSCICTAVNAGIDMVMVPFRYEEFIKDLLSLVESGEIPIARIDDAVERILRVKFV 373
           RGSNYRSCICTAVNAGIDMVMVPFRYEEFIKDLLSLVESGEIPIARIDDAVERILRVKFV
Sbjct: 301 RGSNYRSCICTAVNAGIDMVMVPFRYEEFIKDLLSLVESGEIPIARIDDAVERILRVKFV 360

Query: 374 AGLFEHPFSDRSLIDVVGCKIHRDLAREAVRKSLVLLRNGKDPMKPFLPLDRKAKKILVA 433
           AGLFEHPFSDRSLIDVVGCKIHRDLAREAVRKSLVLLRNGKDPMKPFLPLDRKAKKILVA
Sbjct: 361 AGLFEHPFSDRSLIDVVGCKIHRDLAREAVRKSLVLLRNGKDPMKPFLPLDRKAKKILVA 420

Query: 434 GSHADDLGYQCGGWTISWNGSTGRTTVGTTILDAIKEAVGDQTKVIYEQNPSAVTLNDQD 493
           GSHADDLGYQCGGWTISWNGSTGRTTVGTTILDAIKEAVGDQTKVIYEQNPSAVTLNDQD
Sbjct: 421 GSHADDLGYQCGGWTISWNGSTGRTTVGTTILDAIKEAVGDQTKVIYEQNPSAVTLNDQD 480

Query: 494 ISFAIVAIGESPYAESAGDNSKLIIPFNGNEIVKAVAGKIPTLVILISGRPLVLEPTVIE 553
           ISFAIVAIGESPYAESAGDNSKLIIPFNGNEIVKAVAGKIPTLVILISGRPLVLEPTVIE
Sbjct: 481 ISFAIVAIGESPYAESAGDNSKLIIPFNGNEIVKAVAGKIPTLVILISGRPLVLEPTVIE 540

Query: 554 NVEALIAAWLPGTEGNGITDVIFGDYDFTGRLPVTWFKTVEQLPVHAENNLQDSLFPFGF 613
           NVEALIAAWLPGTEGNGITDVIFGDYDFTGRLPVTWFKTVEQLPVHAENNLQDSLFPFGF
Sbjct: 541 NVEALIAAWLPGTEGNGITDVIFGDYDFTGRLPVTWFKTVEQLPVHAENNLQDSLFPFGF 600

Query: 614 GLSYGKEISSL 625
           GLSYGKEISSL
Sbjct: 601 GLSYGKEISSL 611

BLAST of Cucsa.146880 vs. TrEMBL
Match: A0A0A0LV38_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_1G528550 PE=3 SV=1)

HSP 1 Score: 1064.7 bits (2752), Expect = 4.3e-308
Identity = 520/604 (86.09%), Postives = 559/604 (92.55%), Query Frame = 1

Query: 19  DCMYRNPGAAIEDRIKDLLSRMSLREKIGQMTQIERSVVTPSALTDLAVGSVLSGGDNPP 78
           DC+Y+N  A IE RIKDLLSRM+LREKIGQMTQIER+V TPSAL D A+GSVL+ G + P
Sbjct: 5   DCVYKNSSAPIEVRIKDLLSRMTLREKIGQMTQIERTVATPSALGDFAIGSVLNAGGSAP 64

Query: 79  FDKAMSLDWADMVDGFQSLALQSRLGIPIIYGIDAVHGSSNVYGATIFPHNVGLGATRDG 138
           F  A+S DWADM+D FQS A+QSRLGIPIIYG DAVHG++NVYGATIFPHNVGLGATRD 
Sbjct: 65  FRGALSSDWADMIDRFQSWAIQSRLGIPIIYGSDAVHGNNNVYGATIFPHNVGLGATRDA 124

Query: 139 KLVRRIGTVTALEVRASGVHYAFAPCLAVSRDPRWGRCYESYSEHTEVVRKMTSLVEGLQ 198
            LVRRIGTVTALEVRASG+HYAFAPC+AVSRDPRWGRCYESYSE TEVVRKMT LVEGLQ
Sbjct: 125 DLVRRIGTVTALEVRASGIHYAFAPCVAVSRDPRWGRCYESYSEDTEVVRKMTCLVEGLQ 184

Query: 199 GKPPEGYPKGYPFVAGRNNVIACAKHFVGDGGTDKGLNEGNTIIDSYDELERIHIAPYLD 258
           GKPP GYPKGYPFVAGRNNVIACAKHFVGDGGTDKGLNEGNTI  SYDELERIH+APYLD
Sbjct: 185 GKPPTGYPKGYPFVAGRNNVIACAKHFVGDGGTDKGLNEGNTIA-SYDELERIHMAPYLD 244

Query: 259 CIAQGLSTVMASYSSWNGNPLHTHHFLLTQVLKEKLGFKGFVISDWEALDRLSNPRGSNY 318
           CIAQG+STVMASYSSWNG PLH  HFLLTQ+LK KLGFKGFVISDW+ LDRLS PRGSNY
Sbjct: 245 CIAQGVSTVMASYSSWNGRPLHADHFLLTQILKNKLGFKGFVISDWQGLDRLSRPRGSNY 304

Query: 319 RSCICTAVNAGIDMVMVPFRYEEFIKDLLSLVESGEIPIARIDDAVERILRVKFVAGLFE 378
           R CI  AVNAGIDMVMVP RYE+FIKDLL LVESGEIP+ RIDDAVERILRVKFV+G+FE
Sbjct: 305 RLCISAAVNAGIDMVMVPLRYEQFIKDLLFLVESGEIPMTRIDDAVERILRVKFVSGVFE 364

Query: 379 HPFSDRSLIDVVGCKIHRDLAREAVRKSLVLLRNGKDPMKPFLPLDRKAKKILVAGSHAD 438
           HPFSDRSL+DVVGCKIHRDLAREAVRKSLVLL+NGKDP KPFLPLD KAKKILVAGSHAD
Sbjct: 365 HPFSDRSLLDVVGCKIHRDLAREAVRKSLVLLKNGKDPTKPFLPLDMKAKKILVAGSHAD 424

Query: 439 DLGYQCGGWTISWNGSTGRTTVGTTILDAIKEAVGDQTKVIYEQNPSAVTLNDQDISFAI 498
           DLGYQCGGWTISW+G TGR T+GTTILDAIKEAVGDQT+VIYEQNPSA TLNDQDISFAI
Sbjct: 425 DLGYQCGGWTISWDGMTGRITIGTTILDAIKEAVGDQTEVIYEQNPSAATLNDQDISFAI 484

Query: 499 VAIGESPYAESAGDNSKLIIPFNGNEIVKAVAGKIPTLVILISGRPLVLEPTVIENVEAL 558
           VAIGESPYAE  GD+SKL+IPFNGN+IVKAVAGK+PTLVIL+SGRPL+LEPTV+EN EAL
Sbjct: 485 VAIGESPYAEFTGDDSKLVIPFNGNDIVKAVAGKMPTLVILVSGRPLILEPTVMENAEAL 544

Query: 559 IAAWLPGTEGNGITDVIFGDYDFTGRLPVTWFKTVEQLPVHAENNLQDSLFPFGFGLSYG 618
           IAAWLPG+EG+GITDVIFGDYDFTGRLP+TWF+TVEQLPVHAENNLQ+SLFPFGFGLSY 
Sbjct: 545 IAAWLPGSEGSGITDVIFGDYDFTGRLPITWFRTVEQLPVHAENNLQESLFPFGFGLSYD 604

Query: 619 KEIS 623
           KE S
Sbjct: 605 KEKS 607

BLAST of Cucsa.146880 vs. TrEMBL
Match: F6GUU3_VITVI (Putative uncharacterized protein OS=Vitis vinifera GN=VIT_06s0004g06110 PE=3 SV=1)

HSP 1 Score: 964.5 bits (2492), Expect = 6.1e-278
Identity = 453/604 (75.00%), Postives = 530/604 (87.75%), Query Frame = 1

Query: 19  DCMYRNPGAAIEDRIKDLLSRMSLREKIGQMTQIERSVVTPSALTDLAVGSVLSGGDNPP 78
           DC+Y++P   IE RIKDLLSRM+L+EK GQMTQIER V TPS L DL++GS+LS G + P
Sbjct: 2   DCIYKDPNQPIEARIKDLLSRMTLKEKAGQMTQIERRVATPSVLKDLSIGSILSAGGSGP 61

Query: 79  FDKAMSLDWADMVDGFQSLALQSRLGIPIIYGIDAVHGSSNVYGATIFPHNVGLGATRDG 138
           FDKA+S DWADMVDGFQ  AL+SRLGIP++YGIDAVHG++++YGATIFPHNVGLGATRD 
Sbjct: 62  FDKALSADWADMVDGFQQSALESRLGIPLLYGIDAVHGNNSIYGATIFPHNVGLGATRDA 121

Query: 139 KLVRRIGTVTALEVRASGVHYAFAPCLAVSRDPRWGRCYESYSEHTEVVRKMTSLVEGLQ 198
            L +RIG  TALEVRASG+HY FAPC+AV RDPRWGRCYESYS  T +VRKMTS++ GLQ
Sbjct: 122 DLAQRIGVATALEVRASGIHYTFAPCVAVCRDPRWGRCYESYSSDTNIVRKMTSVITGLQ 181

Query: 199 GKPPEGYPKGYPFVAGRNNVIACAKHFVGDGGTDKGLNEGNTIIDSYDELERIHIAPYLD 258
           GKPP G+PKGYPFVAGR+NV+ACAKHFVGDGGTDKG NEGNTI+ SY++LERIH+ PY D
Sbjct: 182 GKPPPGHPKGYPFVAGRHNVVACAKHFVGDGGTDKGENEGNTIL-SYEDLERIHMTPYPD 241

Query: 259 CIAQGLSTVMASYSSWNGNPLHTHHFLLTQVLKEKLGFKGFVISDWEALDRLS--NPRGS 318
           CI+QG++TVMASYSSWNG  LH H FLL+ VLK+K+GFKGF+ISDWE LDRLS  NP GS
Sbjct: 242 CISQGVATVMASYSSWNGTQLHAHRFLLSDVLKDKMGFKGFLISDWEGLDRLSKPNPHGS 301

Query: 319 NYRSCICTAVNAGIDMVMVPFRYEEFIKDLLSLVESGEIPIARIDDAVERILRVKFVAGL 378
           NYR+ ICTAVN GIDMVMVPFRY +F++DL+ LVESGEIP+ RIDDAVERILRVK VAGL
Sbjct: 302 NYRTSICTAVNTGIDMVMVPFRYAKFLEDLIDLVESGEIPMTRIDDAVERILRVKLVAGL 361

Query: 379 FEHPFSDRSLIDVVGCKIHRDLAREAVRKSLVLLRNGKDPMKPFLPLDRKAKKILVAGSH 438
           FE+P+SDRSL+D VGCK+HRDLAREAVRKSLVLL+NGKD  KPFLPLDRKAK++LVAGSH
Sbjct: 362 FEYPYSDRSLLDTVGCKLHRDLAREAVRKSLVLLKNGKDQKKPFLPLDRKAKRVLVAGSH 421

Query: 439 ADDLGYQCGGWTISWNGSTGRTTVGTTILDAIKEAVGDQTKVIYEQNPSAVTLNDQDISF 498
           ADDLGYQCGGWT +W+G++GR T+GTT+LDAI+EAVGD+T+VIYEQNPS  T   QD S+
Sbjct: 422 ADDLGYQCGGWTATWHGASGRITIGTTVLDAIREAVGDKTEVIYEQNPSPATFEGQDFSY 481

Query: 499 AIVAIGESPYAESAGDNSKLIIPFNGNEIVKAVAGKIPTLVILISGRPLVLEPTVIENVE 558
           AIV +GE PYAE  GDNS+LIIPFN N+++  VA +IPTLVILISGRPLVLEP ++E ++
Sbjct: 482 AIVVVGEDPYAEHTGDNSELIIPFNANDVISLVADRIPTLVILISGRPLVLEPWILEKMD 541

Query: 559 ALIAAWLPGTEGNGITDVIFGDYDFTGRLPVTWFKTVEQLPVHAENNLQDSLFPFGFGLS 618
           ALIAAWLPG+EG GITDV+FGDYDF GRLPVTWFK+VEQLP+H E+N  D LFPFGFGL+
Sbjct: 542 ALIAAWLPGSEGGGITDVVFGDYDFEGRLPVTWFKSVEQLPMHPEDNSYDPLFPFGFGLT 601

Query: 619 YGKE 621
           Y K+
Sbjct: 602 YNKK 604

BLAST of Cucsa.146880 vs. TrEMBL
Match: A5BKF4_VITVI (Putative uncharacterized protein OS=Vitis vinifera GN=VITISV_033665 PE=3 SV=1)

HSP 1 Score: 946.4 bits (2445), Expect = 1.7e-272
Identity = 452/635 (71.18%), Postives = 530/635 (83.46%), Query Frame = 1

Query: 19  DCMYRNPGAAIEDRIKDLLSRMSLREKIGQMTQIERSVVTPSALTDLAVG---------- 78
           DC+Y++P   IE RIKDLLSRM+L+EK GQMTQIER V TPS L DL++G          
Sbjct: 2   DCIYKDPNQPIEARIKDLLSRMTLKEKAGQMTQIERRVATPSVLKDLSIGTIHLIMQYAL 61

Query: 79  ---------------------SVLSGGDNPPFDKAMSLDWADMVDGFQSLALQSRLGIPI 138
                                S+LS G + PFDKA+S DWADMVDGFQ  AL+SRLGIP+
Sbjct: 62  MDCVLLCIFFIQLVVLILFSGSILSAGGSGPFDKALSADWADMVDGFQKSALESRLGIPL 121

Query: 139 IYGIDAVHGSSNVYGATIFPHNVGLGATRDGKLVRRIGTVTALEVRASGVHYAFAPCLAV 198
           +YGIDAVHG++++YGATIFPHNVGLGATRD  L +RIG  TALEVRASG+HY FAPC+AV
Sbjct: 122 LYGIDAVHGNNSIYGATIFPHNVGLGATRDADLAQRIGVATALEVRASGIHYTFAPCVAV 181

Query: 199 SRDPRWGRCYESYSEHTEVVRKMTSLVEGLQGKPPEGYPKGYPFVAGRNNVIACAKHFVG 258
            RDPRWGRCYES S  T +VRKMTS++ GLQGKPP G+PKGYPFVAGR+NV+ACAKHFVG
Sbjct: 182 CRDPRWGRCYESXSSDTNIVRKMTSVITGLQGKPPPGHPKGYPFVAGRHNVVACAKHFVG 241

Query: 259 DGGTDKGLNEGNTIIDSYDELERIHIAPYLDCIAQGLSTVMASYSSWNGNPLHTHHFLLT 318
           DGGTDKG NEGNTI+ SY++LERIH+ PY DCI+QG++TVMASYSSWNG  LH H FLL+
Sbjct: 242 DGGTDKGENEGNTIL-SYEDLERIHMTPYPDCISQGVATVMASYSSWNGTQLHAHRFLLS 301

Query: 319 QVLKEKLGFKGFVISDWEALDRLS--NPRGSNYRSCICTAVNAGIDMVMVPFRYEEFIKD 378
            VLK+K+GFKGF+ISDWE LDRLS  NP GSNYR+ ICTAVN GIDMVMVPFRY +F++D
Sbjct: 302 DVLKDKMGFKGFLISDWEGLDRLSKPNPHGSNYRTSICTAVNTGIDMVMVPFRYAKFLED 361

Query: 379 LLSLVESGEIPIARIDDAVERILRVKFVAGLFEHPFSDRSLIDVVGCKIHRDLAREAVRK 438
           L+ LVESGEIP+ RIDDAVERILRVKFVAGLFE+P+SDRSL+D VGCK+HRDLAREAVRK
Sbjct: 362 LIDLVESGEIPMTRIDDAVERILRVKFVAGLFEYPYSDRSLLDTVGCKLHRDLAREAVRK 421

Query: 439 SLVLLRNGKDPMKPFLPLDRKAKKILVAGSHADDLGYQCGGWTISWNGSTGRTTVGTTIL 498
           SLVLL+NGKD  KPFLPLDRKAK++LVAGSHADDLGYQCGGWT +W+G++GR T+GTT+L
Sbjct: 422 SLVLLKNGKDQKKPFLPLDRKAKRVLVAGSHADDLGYQCGGWTATWHGASGRITIGTTVL 481

Query: 499 DAIKEAVGDQTKVIYEQNPSAVTLNDQDISFAIVAIGESPYAESAGDNSKLIIPFNGNEI 558
           DAI+EAVGD+T+VIYEQNPS  T   QD S+AIV +GE PYAE  GDNS+LIIPFN N++
Sbjct: 482 DAIREAVGDKTEVIYEQNPSPATFEGQDFSYAIVVVGEDPYAEHTGDNSELIIPFNANDV 541

Query: 559 VKAVAGKIPTLVILISGRPLVLEPTVIENVEALIAAWLPGTEGNGITDVIFGDYDFTGRL 618
           +  VA +IPTLVILISGRPLVLEP ++E ++ALIAAWLPG+EG G+TDV+FGDYDF GRL
Sbjct: 542 ISLVADRIPTLVILISGRPLVLEPWILEKMDALIAAWLPGSEGGGMTDVVFGDYDFEGRL 601

Query: 619 PVTWFKTVEQLPVHAENNLQDSLFPFGFGLSYGKE 621
           PVTWFK+VEQLP+H E+N  D LFPFGFGL+Y K+
Sbjct: 602 PVTWFKSVEQLPMHPEDNSYDPLFPFGFGLTYNKK 635

BLAST of Cucsa.146880 vs. TrEMBL
Match: M5WGE3_PRUPE (Uncharacterized protein OS=Prunus persica GN=PRUPE_ppa003012mg PE=3 SV=1)

HSP 1 Score: 944.1 bits (2439), Expect = 8.5e-272
Identity = 446/601 (74.21%), Postives = 527/601 (87.69%), Query Frame = 1

Query: 19  DCMYRNPGAAIEDRIKDLLSRMSLREKIGQMTQIERSVVTPSALTDLAVGSVLSGGDNPP 78
           +C+YRNP   +E R+KDLLSRM+L+EK+GQMTQIER V TP A+ D ++GSVLS G + P
Sbjct: 8   NCIYRNPNEPVEARVKDLLSRMTLKEKVGQMTQIERRVSTPDAIRDFSIGSVLSAGGSVP 67

Query: 79  FDKAMSLDWADMVDGFQSLALQSRLGIPIIYGIDAVHGSSNVYGATIFPHNVGLGATRDG 138
           F+KA+S DWADMVDGFQ  AL+SRLGIP+IYGIDAVHG+++VYGATIFPHNVGLGATRD 
Sbjct: 68  FEKALSSDWADMVDGFQRSALESRLGIPLIYGIDAVHGNNSVYGATIFPHNVGLGATRDA 127

Query: 139 KLVRRIGTVTALEVRASGVHYAFAPCLAVSRDPRWGRCYESYSEHTEVVRKMTSLVEGLQ 198
            LV+RIG  TALEVRASG+HY FAPC+AV RDPRWGRCYESYSE TE+VRKMTS+V GLQ
Sbjct: 128 DLVKRIGAATALEVRASGIHYTFAPCVAVCRDPRWGRCYESYSEDTEIVRKMTSIVTGLQ 187

Query: 199 GKPPEGYPKGYPFVAGRNNVIACAKHFVGDGGTDKGLNEGNTIIDSYDELERIHIAPYLD 258
           G+PP+GYPKGYPFV GRNN IACAKHFVGDGGT KGLNEGNTI  SYD+LERIH+APYL+
Sbjct: 188 GQPPQGYPKGYPFVLGRNNTIACAKHFVGDGGTHKGLNEGNTI-SSYDDLERIHMAPYLN 247

Query: 259 CIAQGLSTVMASYSSWNGNPLHTHHFLLTQVLKEKLGFKGFVISDWEALDRLSNPRGSNY 318
           CI+ G+STVMASYSSWNG+ LH   FLLT++LK+KLGFKGFVISDWEALD+L  PRG++Y
Sbjct: 248 CISDGVSTVMASYSSWNGSKLHADRFLLTEILKDKLGFKGFVISDWEALDQLCEPRGADY 307

Query: 319 RSCICTAVNAGIDMVMVPFRYEEFIKDLLSLVESGEIPIARIDDAVERILRVKFVAGLFE 378
           R CI +AVNAGIDMVMVPFRYE+F+KDL+ LVE G I ++RIDDAVERILRVKFV+GLFE
Sbjct: 308 RFCISSAVNAGIDMVMVPFRYEQFVKDLVYLVEHGNISMSRIDDAVERILRVKFVSGLFE 367

Query: 379 HPFSDRSLIDVVGCKIHRDLAREAVRKSLVLLRNGKDPMKPFLPLDRKAKKILVAGSHAD 438
           HPFSDRSL+D+VGCK+HRDLAREAVRKSLVLL+NGKD  KPFLPLDRKAK+ILVAG+HAD
Sbjct: 368 HPFSDRSLLDMVGCKLHRDLAREAVRKSLVLLKNGKDSRKPFLPLDRKAKRILVAGTHAD 427

Query: 439 DLGYQCGGWTISWNGSTGRTTVGTTILDAIKEAVGDQTKVIYEQNPSAVTLNDQDISFAI 498
           DLGYQCGGWT +W+G +GR T GTT+L+AI++AVGD T++IYEQ PSA TL  +DISFAI
Sbjct: 428 DLGYQCGGWTATWDGRSGRITTGTTVLEAIQKAVGDDTEIIYEQYPSADTLAREDISFAI 487

Query: 499 VAIGESPYAESAGDNSKLIIPFNGNEIVKAVAGKIPTLVILISGRPLVLEPTVIENVEAL 558
           VA+GE PYAE  GDN +L IPFNG +++ +VA ++PTLVILISGRPL LEP ++E ++AL
Sbjct: 488 VAVGEGPYAEFRGDNLELAIPFNGTDVISSVADRLPTLVILISGRPLTLEPWLLEKMDAL 547

Query: 559 IAAWLPGTEGNGITDVIFGDYDFTGRLPVTWFKTVEQLPVHAENNLQDSLFPFGFGLSYG 618
           +AAWLPG+EG GI DVIFGDYDF G LPV+WFK VEQLP++A +N  D L+P G+GL+Y 
Sbjct: 548 VAAWLPGSEGEGIADVIFGDYDFEGLLPVSWFKRVEQLPMNALDNSYDPLYPLGYGLTYN 607

Query: 619 K 620
           K
Sbjct: 608 K 607

BLAST of Cucsa.146880 vs. TAIR10
Match: AT3G47000.1 (AT3G47000.1 Glycosyl hydrolase family protein)

HSP 1 Score: 907.1 bits (2343), Expect = 5.9e-264
Identity = 439/601 (73.04%), Postives = 511/601 (85.02%), Query Frame = 1

Query: 17  KNDCMYRNPGAAIEDRIKDLLSRMSLREKIGQMTQIERSVVTPSALTDLAVGSVLSGGDN 76
           ++ C+Y+N  A +E R+KDLLSRM+L EKIGQMTQIER V +PSA TD  +GSVL+ G +
Sbjct: 5   ESSCVYKNGDAPVEARVKDLLSRMTLPEKIGQMTQIERRVASPSAFTDFFIGSVLNAGGS 64

Query: 77  PPFDKAMSLDWADMVDGFQSLALQSRLGIPIIYGIDAVHGSSNVYGATIFPHNVGLGATR 136
            PF+ A S DWADM+DGFQ  AL SRLGIPIIYG DAVHG++NVYGAT+FPHN+GLGATR
Sbjct: 65  VPFEDAKSSDWADMIDGFQRSALASRLGIPIIYGTDAVHGNNNVYGATVFPHNIGLGATR 124

Query: 137 DGKLVRRIGTVTALEVRASGVHYAFAPCLAVSRDPRWGRCYESYSEHTEVVRKMTSLVEG 196
           D  LVRRIG  TALEVRASGVH+AF+PC+AV RDPRWGRCYESY E  E+V +MTSLV G
Sbjct: 125 DADLVRRIGAATALEVRASGVHWAFSPCVAVLRDPRWGRCYESYGEDPELVCEMTSLVSG 184

Query: 197 LQGKPPEGYPKGYPFVAGRNNVIACAKHFVGDGGTDKGLNEGNTIIDSYDELERIHIAPY 256
           LQG PPE +P GYPFVAGRNNV+AC KHFVGDGGTDKG+NEGNTI  SY+ELE+IHI PY
Sbjct: 185 LQGVPPEEHPNGYPFVAGRNNVVACVKHFVGDGGTDKGINEGNTIA-SYEELEKIHIPPY 244

Query: 257 LDCIAQGLSTVMASYSSWNGNPLHTHHFLLTQVLKEKLGFKGFVISDWEALDRLSNPRGS 316
           L C+AQG+STVMASYSSWNG  LH   FLLT++LKEKLGFKGF++SDWE LDRLS P+GS
Sbjct: 245 LKCLAQGVSTVMASYSSWNGTRLHADRFLLTEILKEKLGFKGFLVSDWEGLDRLSEPQGS 304

Query: 317 NYRSCICTAVNAGIDMVMVPFRYEEFIKDLLSLVESGEIPIARIDDAVERILRVKFVAGL 376
           NYR CI TAVNAGIDMVMVPF+YE+FI+D+  LVESGEIP+ARI+DAVERILRVKFVAGL
Sbjct: 305 NYRYCIKTAVNAGIDMVMVPFKYEQFIQDMTDLVESGEIPMARINDAVERILRVKFVAGL 364

Query: 377 FEHPFSDRSLIDVVGCKIHRDLAREAVRKSLVLLRNGKDPMKPFLPLDRKAKKILVAGSH 436
           F HP +DRSL+  VGCK HR+LA+EAVRKSLVLL++GK+  KPFLPLDR AK+ILV G+H
Sbjct: 365 FGHPLTDRSLLPTVGCKEHRELAQEAVRKSLVLLKSGKNADKPFLPLDRNAKRILVTGTH 424

Query: 437 ADDLGYQCGGWTISWNGSTGRTTVGTTILDAIKEAVGDQTKVIYEQNPSAVTL-NDQDIS 496
           ADDLGYQCGGWT +W G +GR T+GTT+LDAIKEAVGD+T+VIYE+ PS  TL + +  S
Sbjct: 425 ADDLGYQCGGWTKTWFGLSGRITIGTTLLDAIKEAVGDETEVIYEKTPSKETLASSEGFS 484

Query: 497 FAIVAIGESPYAESAGDNSKLIIPFNGNEIVKAVAGKIPTLVILISGRPLVLEPTVIENV 556
           +AIVA+GE PYAE+ GDNS+L IPFNG +IV AVA  IPTLVILISGRP+VLEPTV+E  
Sbjct: 485 YAIVAVGEPPYAETMGDNSELRIPFNGTDIVTAVAEIIPTLVILISGRPVVLEPTVLEKT 544

Query: 557 EALIAAWLPGTEGNGITDVIFGDYDFTGRLPVTWFKTVEQLPVHAENNLQDSLFPFGFGL 616
           EAL+AAWLPGTEG G+ DV+FGDYDF G+LPV+WFK VE LP+ A  N  D LFPFGFGL
Sbjct: 545 EALVAAWLPGTEGQGVADVVFGDYDFKGKLPVSWFKHVEHLPLDAHANSYDPLFPFGFGL 604

BLAST of Cucsa.146880 vs. TAIR10
Match: AT3G47010.1 (AT3G47010.1 Glycosyl hydrolase family protein)

HSP 1 Score: 866.7 bits (2238), Expect = 8.8e-252
Identity = 414/597 (69.35%), Postives = 504/597 (84.42%), Query Frame = 1

Query: 21  MYRNPGAAIEDRIKDLLSRMSLREKIGQMTQIERSVVTPSALTDLAVGSVLSGGDNPPFD 80
           +Y+N  A +E R+KDLLSRM+L EKIGQMTQIERSV +P  +T+  +GSV SG  + P +
Sbjct: 10  VYKNRDAPVEARVKDLLSRMTLPEKIGQMTQIERSVASPQVITNSFIGSVQSGAGSWPLE 69

Query: 81  KAMSLDWADMVDGFQSLALQSRLGIPIIYGIDAVHGSSNVYGATIFPHNVGLGATRDGKL 140
            A S DWADM+DGFQ  AL SRLGIPIIYG DAVHG++NVYGAT+FPHN+GLGATRD  L
Sbjct: 70  DAKSSDWADMIDGFQRSALASRLGIPIIYGTDAVHGNNNVYGATVFPHNIGLGATRDADL 129

Query: 141 VRRIGTVTALEVRASGVHYAFAPCLAVSRDPRWGRCYESYSEHTEVVRKMTSLVEGLQGK 200
           V+RIG  TALE+RASGVH+ FAPC+AV  DPRWGRCYESYSE  ++V +M+ L+ GLQG+
Sbjct: 130 VKRIGAATALEIRASGVHWTFAPCVAVLGDPRWGRCYESYSEAAKIVCEMSLLISGLQGE 189

Query: 201 PPEGYPKGYPFVAGRNNVIACAKHFVGDGGTDKGLNEGNTIIDSYDELERIHIAPYLDCI 260
           PPE +P GYPF+AGRNNVIACAKHFVGDGGT+KGL+EGNTI  SY++LE+IH+APYL+CI
Sbjct: 190 PPEEHPYGYPFLAGRNNVIACAKHFVGDGGTEKGLSEGNTIT-SYEDLEKIHVAPYLNCI 249

Query: 261 AQGLSTVMASYSSWNGNPLHTHHFLLTQVLKEKLGFKGFVISDWEALDRLSNPRGSNYRS 320
           AQG+STVMAS+SSWNG+ LH+ +FLLT+VLK+KLGFKGF++SDW+ L+ +S P GSNYR+
Sbjct: 250 AQGVSTVMASFSSWNGSRLHSDYFLLTEVLKQKLGFKGFLVSDWDGLETISEPEGSNYRN 309

Query: 321 CICTAVNAGIDMVMVPFRYEEFIKDLLSLVESGEIPIARIDDAVERILRVKFVAGLFEHP 380
           C+   +NAGIDMVMVPF+YE+FI+D+  LVESGEIP+AR++DAVERILRVKFVAGLFEHP
Sbjct: 310 CVKLGINAGIDMVMVPFKYEQFIQDMTDLVESGEIPMARVNDAVERILRVKFVAGLFEHP 369

Query: 381 FSDRSLIDVVGCKIHRDLAREAVRKSLVLLRNGKDPMKPFLPLDRKAKKILVAGSHADDL 440
            +DRSL+  VGCK HR++AREAVRKSLVLL+NGK+   PFLPLDR AK+ILV G HA+DL
Sbjct: 370 LADRSLLGTVGCKEHREVAREAVRKSLVLLKNGKNADTPFLPLDRNAKRILVVGMHANDL 429

Query: 441 GYQCGGWTISWNGSTGRTTVGTTILDAIKEAVGDQTKVIYEQNPSAVTLNDQD-ISFAIV 500
           G QCGGWT   +G +GR T+GTT+LD+IK AVGD+T+VI+E+ P+  TL   D  S+AIV
Sbjct: 430 GNQCGGWTKIKSGQSGRITIGTTLLDSIKAAVGDKTEVIFEKTPTKETLASSDGFSYAIV 489

Query: 501 AIGESPYAESAGDNSKLIIPFNGNEIVKAVAGKIPTLVILISGRPLVLEPTVIENVEALI 560
           A+GE PYAE  GDNS+L IPFNGN I+ AVA KIPTLVIL SGRP+VLEPTV+E  EAL+
Sbjct: 490 AVGEPPYAEMKGDNSELTIPFNGNNIITAVAEKIPTLVILFSGRPMVLEPTVLEKTEALV 549

Query: 561 AAWLPGTEGNGITDVIFGDYDFTGRLPVTWFKTVEQLPVHAENNLQDSLFPFGFGLS 617
           AAW PGTEG G++DVIFGDYDF G+LPV+WFK V+QLP++AE N  D LFP GFGL+
Sbjct: 550 AAWFPGTEGQGMSDVIFGDYDFKGKLPVSWFKRVDQLPLNAEANSYDPLFPLGFGLT 605

BLAST of Cucsa.146880 vs. TAIR10
Match: AT3G47040.2 (AT3G47040.2 Glycosyl hydrolase family protein)

HSP 1 Score: 854.0 bits (2205), Expect = 5.9e-248
Identity = 423/639 (66.20%), Postives = 513/639 (80.28%), Query Frame = 1

Query: 14  MESKND-CMYRNPGAAIEDRIKDLLSRMSLREKIGQMTQIERSVVTPSALTDLAVGSVLS 73
           ME  N+ C+Y+N  A +E R+KDLLSRM+L EKIGQMTQIER V TP  +TD  +GSVL+
Sbjct: 1   MEGSNETCVYKNKDAPVEARVKDLLSRMTLPEKIGQMTQIERVVTTPPVITDNFIGSVLN 60

Query: 74  GGDNPPFDKAMSLDWADMVDGFQSLALQSRLGIPIIYGIDAVHGSSNVYGATIFPHNVGL 133
           GG + PF+ A + DWADM+DG+Q+ AL SRLGIPIIYGIDAVHG++NVYGATIFPHN+GL
Sbjct: 61  GGGSWPFEDAKTSDWADMIDGYQNAALASRLGIPIIYGIDAVHGNNNVYGATIFPHNIGL 120

Query: 134 GAT-------------------------RDGKLVRRIGTVTALEVRASGVHYAFAPCLAV 193
           GAT                         RD  L+RR+G  TALEVRA G H+AFAPC+A 
Sbjct: 121 GATSLVMLLHIDLEPKSLGRNKVVVKCDRDADLIRRVGAATALEVRACGAHWAFAPCVAT 180

Query: 194 SRDPRWG-----RCY---ESYSEHTEVVRKMTSLVEGLQGKPPEGYPKGYPFVAGRNNVI 253
           S   R       + Y   E   E  +++ +++SLV GLQG+PP+ +P GYPF+AGRNNV+
Sbjct: 181 SIQGRIPNKKIKKIYMRKELKCEDPDIICELSSLVSGLQGEPPKEHPNGYPFLAGRNNVV 240

Query: 254 ACAKHFVGDGGTDKGLNEGNTIIDSYDELERIHIAPYLDCIAQGLSTVMASYSSWNGNPL 313
           ACAKHFVGDGGTDKG+NEGNTI+ SY+ELE+IH+APYL+C+AQG+STVMASYSSWNG+ L
Sbjct: 241 ACAKHFVGDGGTDKGINEGNTIV-SYEELEKIHLAPYLNCLAQGVSTVMASYSSWNGSKL 300

Query: 314 HTHHFLLTQVLKEKLGFKGFVISDWEALDRLSNPRGSNYRSCICTAVNAGIDMVMVPFRY 373
           H+ +FLLT++LK+KLGFKGFVISDWEAL+RLS P GSNYR+C+  +VNAG+DMVMVPF+Y
Sbjct: 301 HSDYFLLTELLKQKLGFKGFVISDWEALERLSEPFGSNYRNCVKISVNAGVDMVMVPFKY 360

Query: 374 EEFIKDLLSLVESGEIPIARIDDAVERILRVKFVAGLFEHPFSDRSLIDVVGCKIHRDLA 433
           E+FIKDL  LVESGE+ ++RIDDAVERILRVKFVAGLFEHP +DRSL+  VGCK HR+LA
Sbjct: 361 EQFIKDLTDLVESGEVTMSRIDDAVERILRVKFVAGLFEHPLTDRSLLGTVGCKEHRELA 420

Query: 434 REAVRKSLVLLRNGKDPMKPFLPLDRKAKKILVAGSHADDLGYQCGGWTISWNGSTGRTT 493
           RE+VRKSLVLL+NG +  KPFLPLDR  K+ILV G+HADDLGYQCGGWT +W G +GR T
Sbjct: 421 RESVRKSLVLLKNGTNSEKPFLPLDRNVKRILVTGTHADDLGYQCGGWTKAWFGLSGRIT 480

Query: 494 VGTTILDAIKEAVGDQTKVIYEQNPSAVTLND-QDISFAIVAIGESPYAESAGDNSKLII 553
           +GTT+LDAIKEAVGD+T+VIYE+ PS  TL   Q  S+AIVA+GE+PYAE+ GDNS+L I
Sbjct: 481 IGTTLLDAIKEAVGDKTEVIYEKTPSEETLASLQRFSYAIVAVGETPYAETLGDNSELTI 540

Query: 554 PFNGNEIVKAVAGKIPTLVILISGRPLVLEPTVIENVEALIAAWLPGTEGNGITDVIFGD 613
           P NGN+IV A+A KIPTLV+L SGRPLVLEP V+E  EAL+AAWLPGTEG G+TDVIFGD
Sbjct: 541 PLNGNDIVTALAEKIPTLVVLFSGRPLVLEPLVLEKAEALVAAWLPGTEGQGMTDVIFGD 600

Query: 614 YDFTGRLPVTWFKTVEQLPVHAENNLQDSLFPFGFGLSY 618
           YDF G+LPV+WFK V+QLP+ A+ N  D LFP GFGL+Y
Sbjct: 601 YDFEGKLPVSWFKRVDQLPLTADANSYDPLFPLGFGLNY 638

BLAST of Cucsa.146880 vs. TAIR10
Match: AT3G47050.1 (AT3G47050.1 Glycosyl hydrolase family protein)

HSP 1 Score: 839.3 bits (2167), Expect = 1.5e-243
Identity = 406/604 (67.22%), Postives = 499/604 (82.62%), Query Frame = 1

Query: 14  MESKNDCMYRNPGAAIEDRIKDLLSRMSLREKIGQMTQIERSVVTPSALTDLAVGSVLSG 73
           ++++   +Y+N  A +E R+KDLLSRM+L EKIGQMT IERSV + + + D ++GSVL+ 
Sbjct: 2   VDNEKSYVYKNREAPVEARVKDLLSRMTLAEKIGQMTLIERSVASEAVIRDFSIGSVLNR 61

Query: 74  GDNPPFDKAMSLDWADMVDGFQSLALQSRLGIPIIYGIDAVHGSSNVYGATIFPHNVGLG 133
               PF+ A S +WADM+DGFQ  AL+SRLGIPIIYGIDAVHG+++VYGATIFPHN+GLG
Sbjct: 62  AGGWPFEDAKSSNWADMIDGFQRSALESRLGIPIIYGIDAVHGNNDVYGATIFPHNIGLG 121

Query: 134 ATRDGKLVRRIGTVTALEVRASGVHYAFAPCLAVSRDPRWGRCYESYSEHTEVVRKMTSL 193
           ATRD  LV+RIG  TALEVRA G H+AFAPC+AV +DPRWGRCYESY E  ++V +MTSL
Sbjct: 122 ATRDADLVKRIGAATALEVRACGAHWAFAPCVAVVKDPRWGRCYESYGEVAQIVSEMTSL 181

Query: 194 VEGLQGKPPEGYPKGYPFVAGRNNVIACAKHFVGDGGTDKGLNEGNTIIDSYDELERIHI 253
           V GLQG+P + +  GYPF+AGR NV+ACAKHFVGDGGT+K +NEGNTI+  Y++LER HI
Sbjct: 182 VSGLQGEPSKDHTNGYPFLAGRKNVVACAKHFVGDGGTNKAINEGNTIL-RYEDLERKHI 241

Query: 254 APYLDCIAQGLSTVMASYSSWNGNPLHTHHFLLTQVLKEKLGFKGFVISDWEALDRLSNP 313
           APY  CI+QG+STVMASYSSWNG+ LH+H+FLLT++LK+KLGFKG+V+SDWE LDRLS+P
Sbjct: 242 APYKKCISQGVSTVMASYSSWNGDKLHSHYFLLTEILKQKLGFKGYVVSDWEGLDRLSDP 301

Query: 314 RGSNYRSCICTAVNAGIDMVMVPFRYEEFIKDLLSLVESGEIPIARIDDAVERILRVKFV 373
            GSNYR+C+   +NAGIDMVMVPF+YE+F  DL+ LVESGE+ +AR++DAVERILRVKFV
Sbjct: 302 PGSNYRNCVKIGINAGIDMVMVPFKYEQFRNDLIDLVESGEVSMARVNDAVERILRVKFV 361

Query: 374 AGLFEHPFSDRSLIDVVGCKIHRDLAREAVRKSLVLLRNGKDPMKPFLPLDRKAKKILVA 433
           AGLFE P +DRSL+  VGCK HR+LAREAVRKSLVLL+NG+     FLPL+  A++ILV 
Sbjct: 362 AGLFEFPLTDRSLLPTVGCKEHRELAREAVRKSLVLLKNGR--YGEFLPLNCNAERILVV 421

Query: 434 GSHADDLGYQCGGWTISWNGSTGRTTVGTTILDAIKEAVGDQTKVIYEQNPSAVTL-NDQ 493
           G+HADDLGYQCGGWT +  G +GR T GTT+LDAIK AVGD+T+VIYE++PS  TL +  
Sbjct: 422 GTHADDLGYQCGGWTKTMYGQSGRITDGTTLLDAIKAAVGDETEVIYEKSPSEETLASGY 481

Query: 494 DISFAIVAIGESPYAESAGDNSKLIIPFNGNEIVKAVAGKIPTLVILISGRPLVLEPTVI 553
             S+AIVA+GESPYAE+ GDNS+L+IPFNG+EI+  VA KIPTLVIL SGRP+ LEP V+
Sbjct: 482 RFSYAIVAVGESPYAETMGDNSELVIPFNGSEIITTVAEKIPTLVILFSGRPMFLEPQVL 541

Query: 554 ENVEALIAAWLPGTEGNGITDVIFGDYDFTGRLPVTWFKTVEQLPVHAENNLQDSLFPFG 613
           E  EAL+AAWLPGTEG GI DVIFGDYDF G+LP TWFK V+QLP+  E+N    LFP G
Sbjct: 542 EKAEALVAAWLPGTEGQGIADVIFGDYDFRGKLPATWFKRVDQLPLDIESNGYLPLFPLG 601

Query: 614 FGLS 617
           FGL+
Sbjct: 602 FGLN 602

BLAST of Cucsa.146880 vs. TAIR10
Match: AT5G04885.1 (AT5G04885.1 Glycosyl hydrolase family protein)

HSP 1 Score: 693.0 bits (1787), Expect = 1.7e-199
Identity = 334/596 (56.04%), Postives = 437/596 (73.32%), Query Frame = 1

Query: 21  MYRNPGAAIEDRIKDLLSRMSLREKIGQMTQIERSVVTPSALTDLAVGSVLSGGDNPPFD 80
           +Y++P   + DR+ DL  RM+L EKIGQM QI+RSV T + + D  +GSVLSGG + P  
Sbjct: 29  LYKDPKQTVSDRVADLFGRMTLEEKIGQMVQIDRSVATVNIMRDYFIGSVLSGGGSAPLP 88

Query: 81  KAMSLDWADMVDGFQSLALQSRLGIPIIYGIDAVHGSSNVYGATIFPHNVGLGATRDGKL 140
           +A + +W DM++ +Q  AL SRLGIP+IYGIDAVHG +NVY ATIFPHNVGLGATRD  L
Sbjct: 89  EASAQNWVDMINEYQKGALVSRLGIPMIYGIDAVHGHNNVYNATIFPHNVGLGATRDPDL 148

Query: 141 VRRIGTVTALEVRASGVHYAFAPCLAVSRDPRWGRCYESYSEHTEVVRKMTSLVEGLQGK 200
           V+RIG  TA+EVRA+G+ Y FAPC+AV RDPRWGRCYESYSE  +VV  MT ++ GLQG+
Sbjct: 149 VKRIGAATAVEVRATGIPYTFAPCIAVCRDPRWGRCYESYSEDHKVVEDMTDVILGLQGE 208

Query: 201 PPEGYPKGYPFVAGRNNVIACAKHFVGDGGTDKGLNEGNTIIDSYDELERIHIAPYLDCI 260
           PP  Y  G PFV GR+ V ACAKH+VGDGGT +G+NE NT+ D +  L  +H+  Y D +
Sbjct: 209 PPSNYKHGVPFVGGRDKVAACAKHYVGDGGTTRGVNENNTVTDLHGLLS-VHMPAYADAV 268

Query: 261 AQGLSTVMASYSSWNGNPLHTHHFLLTQVLKEKLGFKGFVISDWEALDRLSNPRGSNYRS 320
            +G+STVM SYSSWNG  +H +  L+T  LK  L FKGFVISDW+ +D++S P  ++Y +
Sbjct: 269 YKGVSTVMVSYSSWNGEKMHANTELITGYLKGTLKFKGFVISDWQGVDKISTPPHTHYTA 328

Query: 321 CICTAVNAGIDMVMVPFRYEEFIKDLLSLVESGEIPIARIDDAVERILRVKFVAGLFEHP 380
            +  A+ AGIDMVMVPF + EF+ DL +LV++  IP+ RIDDAV RIL VKF  GLFE+P
Sbjct: 329 SVRAAIQAGIDMVMVPFNFTEFVNDLTTLVKNNSIPVTRIDDAVRRILLVKFTMGLFENP 388

Query: 381 FSDRSLIDVVGCKIHRDLAREAVRKSLVLLRNGKDPMKPFLPLDRKAKKILVAGSHADDL 440
            +D S    +G + HRDLAREAVRKSLVLL+NG +   P LPL RK  KILVAG+HAD+L
Sbjct: 389 LADYSFSSELGSQAHRDLAREAVRKSLVLLKNG-NKTNPMLPLPRKTSKILVAGTHADNL 448

Query: 441 GYQCGGWTISWNGSTG-RTTVGTTILDAIKEAVGDQTKVIYEQNPSAVTLNDQDISFAIV 500
           GYQCGGWTI+W G +G + T GTT+L A+K AV   T+V++ +NP A  +   + ++AI+
Sbjct: 449 GYQCGGWTITWQGFSGNKNTRGTTLLSAVKSAVDQSTEVVFRENPDAEFIKSNNFAYAII 508

Query: 501 AIGESPYAESAGDNSKLIIPFNGNEIVKAVAGKIPTLVILISGRPLVLEPTVIENVEALI 560
           A+GE PYAE+AGD+ KL +   G  I+ +    +  +V++ISGRPLV+EP  + +++AL+
Sbjct: 509 AVGEPPYAETAGDSDKLTMLDPGPAIISSTCQAVKCVVVVISGRPLVMEP-YVASIDALV 568

Query: 561 AAWLPGTEGNGITDVIFGDYDFTGRLPVTWFKTVEQLPVHAENNLQDSLFPFGFGL 616
           AAWLPGTEG GITD +FGD+ F+G+LPVTWF+  EQLP+   +   D LF +G GL
Sbjct: 569 AAWLPGTEGQGITDALFGDHGFSGKLPVTWFRNTEQLPMSYGDTHYDPLFAYGSGL 621

BLAST of Cucsa.146880 vs. NCBI nr
Match: gi|778661657|ref|XP_004150629.2| (PREDICTED: lysosomal beta glucosidase isoform X5 [Cucumis sativus])

HSP 1 Score: 1257.7 bits (3253), Expect = 0.0e+00
Identity = 624/624 (100.00%), Postives = 624/624 (100.00%), Query Frame = 1

Query: 1   MRWMKLGKGKEEEMESKNDCMYRNPGAAIEDRIKDLLSRMSLREKIGQMTQIERSVVTPS 60
           MRWMKLGKGKEEEMESKNDCMYRNPGAAIEDRIKDLLSRMSLREKIGQMTQIERSVVTPS
Sbjct: 1   MRWMKLGKGKEEEMESKNDCMYRNPGAAIEDRIKDLLSRMSLREKIGQMTQIERSVVTPS 60

Query: 61  ALTDLAVGSVLSGGDNPPFDKAMSLDWADMVDGFQSLALQSRLGIPIIYGIDAVHGSSNV 120
           ALTDLAVGSVLSGGDNPPFDKAMSLDWADMVDGFQSLALQSRLGIPIIYGIDAVHGSSNV
Sbjct: 61  ALTDLAVGSVLSGGDNPPFDKAMSLDWADMVDGFQSLALQSRLGIPIIYGIDAVHGSSNV 120

Query: 121 YGATIFPHNVGLGATRDGKLVRRIGTVTALEVRASGVHYAFAPCLAVSRDPRWGRCYESY 180
           YGATIFPHNVGLGATRDGKLVRRIGTVTALEVRASGVHYAFAPCLAVSRDPRWGRCYESY
Sbjct: 121 YGATIFPHNVGLGATRDGKLVRRIGTVTALEVRASGVHYAFAPCLAVSRDPRWGRCYESY 180

Query: 181 SEHTEVVRKMTSLVEGLQGKPPEGYPKGYPFVAGRNNVIACAKHFVGDGGTDKGLNEGNT 240
           SEHTEVVRKMTSLVEGLQGKPPEGYPKGYPFVAGRNNVIACAKHFVGDGGTDKGLNEGNT
Sbjct: 181 SEHTEVVRKMTSLVEGLQGKPPEGYPKGYPFVAGRNNVIACAKHFVGDGGTDKGLNEGNT 240

Query: 241 IIDSYDELERIHIAPYLDCIAQGLSTVMASYSSWNGNPLHTHHFLLTQVLKEKLGFKGFV 300
           IIDSYDELERIHIAPYLDCIAQGLSTVMASYSSWNGNPLHTHHFLLTQVLKEKLGFKGFV
Sbjct: 241 IIDSYDELERIHIAPYLDCIAQGLSTVMASYSSWNGNPLHTHHFLLTQVLKEKLGFKGFV 300

Query: 301 ISDWEALDRLSNPRGSNYRSCICTAVNAGIDMVMVPFRYEEFIKDLLSLVESGEIPIARI 360
           ISDWEALDRLSNPRGSNYRSCICTAVNAGIDMVMVPFRYEEFIKDLLSLVESGEIPIARI
Sbjct: 301 ISDWEALDRLSNPRGSNYRSCICTAVNAGIDMVMVPFRYEEFIKDLLSLVESGEIPIARI 360

Query: 361 DDAVERILRVKFVAGLFEHPFSDRSLIDVVGCKIHRDLAREAVRKSLVLLRNGKDPMKPF 420
           DDAVERILRVKFVAGLFEHPFSDRSLIDVVGCKIHRDLAREAVRKSLVLLRNGKDPMKPF
Sbjct: 361 DDAVERILRVKFVAGLFEHPFSDRSLIDVVGCKIHRDLAREAVRKSLVLLRNGKDPMKPF 420

Query: 421 LPLDRKAKKILVAGSHADDLGYQCGGWTISWNGSTGRTTVGTTILDAIKEAVGDQTKVIY 480
           LPLDRKAKKILVAGSHADDLGYQCGGWTISWNGSTGRTTVGTTILDAIKEAVGDQTKVIY
Sbjct: 421 LPLDRKAKKILVAGSHADDLGYQCGGWTISWNGSTGRTTVGTTILDAIKEAVGDQTKVIY 480

Query: 481 EQNPSAVTLNDQDISFAIVAIGESPYAESAGDNSKLIIPFNGNEIVKAVAGKIPTLVILI 540
           EQNPSAVTLNDQDISFAIVAIGESPYAESAGDNSKLIIPFNGNEIVKAVAGKIPTLVILI
Sbjct: 481 EQNPSAVTLNDQDISFAIVAIGESPYAESAGDNSKLIIPFNGNEIVKAVAGKIPTLVILI 540

Query: 541 SGRPLVLEPTVIENVEALIAAWLPGTEGNGITDVIFGDYDFTGRLPVTWFKTVEQLPVHA 600
           SGRPLVLEPTVIENVEALIAAWLPGTEGNGITDVIFGDYDFTGRLPVTWFKTVEQLPVHA
Sbjct: 541 SGRPLVLEPTVIENVEALIAAWLPGTEGNGITDVIFGDYDFTGRLPVTWFKTVEQLPVHA 600

Query: 601 ENNLQDSLFPFGFGLSYGKEISSL 625
           ENNLQDSLFPFGFGLSYGKEISSL
Sbjct: 601 ENNLQDSLFPFGFGLSYGKEISSL 624

BLAST of Cucsa.146880 vs. NCBI nr
Match: gi|700210693|gb|KGN65789.1| (hypothetical protein Csa_1G528540 [Cucumis sativus])

HSP 1 Score: 1229.9 bits (3181), Expect = 0.0e+00
Identity = 611/611 (100.00%), Postives = 611/611 (100.00%), Query Frame = 1

Query: 14  MESKNDCMYRNPGAAIEDRIKDLLSRMSLREKIGQMTQIERSVVTPSALTDLAVGSVLSG 73
           MESKNDCMYRNPGAAIEDRIKDLLSRMSLREKIGQMTQIERSVVTPSALTDLAVGSVLSG
Sbjct: 1   MESKNDCMYRNPGAAIEDRIKDLLSRMSLREKIGQMTQIERSVVTPSALTDLAVGSVLSG 60

Query: 74  GDNPPFDKAMSLDWADMVDGFQSLALQSRLGIPIIYGIDAVHGSSNVYGATIFPHNVGLG 133
           GDNPPFDKAMSLDWADMVDGFQSLALQSRLGIPIIYGIDAVHGSSNVYGATIFPHNVGLG
Sbjct: 61  GDNPPFDKAMSLDWADMVDGFQSLALQSRLGIPIIYGIDAVHGSSNVYGATIFPHNVGLG 120

Query: 134 ATRDGKLVRRIGTVTALEVRASGVHYAFAPCLAVSRDPRWGRCYESYSEHTEVVRKMTSL 193
           ATRDGKLVRRIGTVTALEVRASGVHYAFAPCLAVSRDPRWGRCYESYSEHTEVVRKMTSL
Sbjct: 121 ATRDGKLVRRIGTVTALEVRASGVHYAFAPCLAVSRDPRWGRCYESYSEHTEVVRKMTSL 180

Query: 194 VEGLQGKPPEGYPKGYPFVAGRNNVIACAKHFVGDGGTDKGLNEGNTIIDSYDELERIHI 253
           VEGLQGKPPEGYPKGYPFVAGRNNVIACAKHFVGDGGTDKGLNEGNTIIDSYDELERIHI
Sbjct: 181 VEGLQGKPPEGYPKGYPFVAGRNNVIACAKHFVGDGGTDKGLNEGNTIIDSYDELERIHI 240

Query: 254 APYLDCIAQGLSTVMASYSSWNGNPLHTHHFLLTQVLKEKLGFKGFVISDWEALDRLSNP 313
           APYLDCIAQGLSTVMASYSSWNGNPLHTHHFLLTQVLKEKLGFKGFVISDWEALDRLSNP
Sbjct: 241 APYLDCIAQGLSTVMASYSSWNGNPLHTHHFLLTQVLKEKLGFKGFVISDWEALDRLSNP 300

Query: 314 RGSNYRSCICTAVNAGIDMVMVPFRYEEFIKDLLSLVESGEIPIARIDDAVERILRVKFV 373
           RGSNYRSCICTAVNAGIDMVMVPFRYEEFIKDLLSLVESGEIPIARIDDAVERILRVKFV
Sbjct: 301 RGSNYRSCICTAVNAGIDMVMVPFRYEEFIKDLLSLVESGEIPIARIDDAVERILRVKFV 360

Query: 374 AGLFEHPFSDRSLIDVVGCKIHRDLAREAVRKSLVLLRNGKDPMKPFLPLDRKAKKILVA 433
           AGLFEHPFSDRSLIDVVGCKIHRDLAREAVRKSLVLLRNGKDPMKPFLPLDRKAKKILVA
Sbjct: 361 AGLFEHPFSDRSLIDVVGCKIHRDLAREAVRKSLVLLRNGKDPMKPFLPLDRKAKKILVA 420

Query: 434 GSHADDLGYQCGGWTISWNGSTGRTTVGTTILDAIKEAVGDQTKVIYEQNPSAVTLNDQD 493
           GSHADDLGYQCGGWTISWNGSTGRTTVGTTILDAIKEAVGDQTKVIYEQNPSAVTLNDQD
Sbjct: 421 GSHADDLGYQCGGWTISWNGSTGRTTVGTTILDAIKEAVGDQTKVIYEQNPSAVTLNDQD 480

Query: 494 ISFAIVAIGESPYAESAGDNSKLIIPFNGNEIVKAVAGKIPTLVILISGRPLVLEPTVIE 553
           ISFAIVAIGESPYAESAGDNSKLIIPFNGNEIVKAVAGKIPTLVILISGRPLVLEPTVIE
Sbjct: 481 ISFAIVAIGESPYAESAGDNSKLIIPFNGNEIVKAVAGKIPTLVILISGRPLVLEPTVIE 540

Query: 554 NVEALIAAWLPGTEGNGITDVIFGDYDFTGRLPVTWFKTVEQLPVHAENNLQDSLFPFGF 613
           NVEALIAAWLPGTEGNGITDVIFGDYDFTGRLPVTWFKTVEQLPVHAENNLQDSLFPFGF
Sbjct: 541 NVEALIAAWLPGTEGNGITDVIFGDYDFTGRLPVTWFKTVEQLPVHAENNLQDSLFPFGF 600

Query: 614 GLSYGKEISSL 625
           GLSYGKEISSL
Sbjct: 601 GLSYGKEISSL 611

BLAST of Cucsa.146880 vs. NCBI nr
Match: gi|659115123|ref|XP_008457398.1| (PREDICTED: lysosomal beta glucosidase-like isoform X4 [Cucumis melo])

HSP 1 Score: 1193.3 bits (3086), Expect = 0.0e+00
Identity = 589/619 (95.15%), Postives = 609/619 (98.38%), Query Frame = 1

Query: 1   MRWMKLGKGKEEEMESKNDCMYRNPGAAIEDRIKDLLSRMSLREKIGQMTQIERSVVTPS 60
           MRWMK+GKGKE  ME K+DC+YRN GAAIEDRIKDLLSRMSLREKIGQMTQIERSVVTPS
Sbjct: 1   MRWMKIGKGKE--MEGKSDCLYRNAGAAIEDRIKDLLSRMSLREKIGQMTQIERSVVTPS 60

Query: 61  ALTDLAVGSVLSGGDNPPFDKAMSLDWADMVDGFQSLALQSRLGIPIIYGIDAVHGSSNV 120
           ALTDLA+GSVL+GG + PFDKA+S DWADMVDGFQSLALQSRLGIPIIYGIDAVHG++NV
Sbjct: 61  ALTDLAIGSVLNGGGSLPFDKALSSDWADMVDGFQSLALQSRLGIPIIYGIDAVHGNNNV 120

Query: 121 YGATIFPHNVGLGATRDGKLVRRIGTVTALEVRASGVHYAFAPCLAVSRDPRWGRCYESY 180
           YGATIFPHNVGLGATRDGKLVRRIG VTALEVRASGVHYAFAPCLAVSRDPRWGRCYESY
Sbjct: 121 YGATIFPHNVGLGATRDGKLVRRIGRVTALEVRASGVHYAFAPCLAVSRDPRWGRCYESY 180

Query: 181 SEHTEVVRKMTSLVEGLQGKPPEGYPKGYPFVAGRNNVIACAKHFVGDGGTDKGLNEGNT 240
           SE TEVVRKMTSLVEGLQGKPP+GYPKGYPFVAGRNNVIACAKHFVGDGGTDKGLNEGNT
Sbjct: 181 SEDTEVVRKMTSLVEGLQGKPPKGYPKGYPFVAGRNNVIACAKHFVGDGGTDKGLNEGNT 240

Query: 241 IIDSYDELERIHIAPYLDCIAQGLSTVMASYSSWNGNPLHTHHFLLTQVLKEKLGFKGFV 300
           IIDSYDELERIH+APYLDCIAQG+STVMASYSSWNGNPLH HHFLLTQVLKEKLGFKGFV
Sbjct: 241 IIDSYDELERIHMAPYLDCIAQGVSTVMASYSSWNGNPLHAHHFLLTQVLKEKLGFKGFV 300

Query: 301 ISDWEALDRLSNPRGSNYRSCICTAVNAGIDMVMVPFRYEEFIKDLLSLVESGEIPIARI 360
           ISDWEALDRLSNPRGSNYRSCICTAVNAGIDMVMVPFRYEEFIKDLLSLVESGEIPIARI
Sbjct: 301 ISDWEALDRLSNPRGSNYRSCICTAVNAGIDMVMVPFRYEEFIKDLLSLVESGEIPIARI 360

Query: 361 DDAVERILRVKFVAGLFEHPFSDRSLIDVVGCKIHRDLAREAVRKSLVLLRNGKDPMKPF 420
           DDAVERILRVKFVAGLFEHPFSDRSLIDVVGCKIHRDLAREAVRKSLVLLRNGKDPMKPF
Sbjct: 361 DDAVERILRVKFVAGLFEHPFSDRSLIDVVGCKIHRDLAREAVRKSLVLLRNGKDPMKPF 420

Query: 421 LPLDRKAKKILVAGSHADDLGYQCGGWTISWNGSTGRTTVGTTILDAIKEAVGDQTKVIY 480
           LPLDRKAKKILVAGSHADDLGYQCGGWTISWNGSTGRTT+GTTILDAIKEAVGDQTKVIY
Sbjct: 421 LPLDRKAKKILVAGSHADDLGYQCGGWTISWNGSTGRTTIGTTILDAIKEAVGDQTKVIY 480

Query: 481 EQNPSAVTLNDQDISFAIVAIGESPYAESAGDNSKLIIPFNGNEIVKAVAGKIPTLVILI 540
           EQNPSAVTL+DQDISFAIVAIGESPYAESAGD+SKLIIPFNGNEIVKAVAGKIPTLVILI
Sbjct: 481 EQNPSAVTLDDQDISFAIVAIGESPYAESAGDDSKLIIPFNGNEIVKAVAGKIPTLVILI 540

Query: 541 SGRPLVLEPTVIENVEALIAAWLPGTEGNGITDVIFGDYDFTGRLPVTWFKTVEQLPVHA 600
           SGRPLVLEPTVIENVEAL+AAWLPG+EG+GITDVIFGDY+F+GRLPVTWFKTVEQLPVHA
Sbjct: 541 SGRPLVLEPTVIENVEALVAAWLPGSEGDGITDVIFGDYNFSGRLPVTWFKTVEQLPVHA 600

Query: 601 ENNLQDSLFPFGFGLSYGK 620
           ENNLQDSLFPFGFGLSYGK
Sbjct: 601 ENNLQDSLFPFGFGLSYGK 617

BLAST of Cucsa.146880 vs. NCBI nr
Match: gi|778661645|ref|XP_011658498.1| (PREDICTED: lysosomal beta glucosidase isoform X2 [Cucumis sativus])

HSP 1 Score: 1094.7 bits (2830), Expect = 0.0e+00
Identity = 541/606 (89.27%), Postives = 567/606 (93.56%), Query Frame = 1

Query: 19  DCMYRNPGAAIEDRIKDLLSRMSLREKIGQMTQIERSVVTPSALTDLAVGSVLSGGDNPP 78
           DC+Y+N  A IE RIKDLLSRM+LREKIGQMTQIER+V TPSAL D A+GSVL+ G + P
Sbjct: 5   DCVYKNSSAPIEVRIKDLLSRMTLREKIGQMTQIERTVATPSALGDFAIGSVLNAGGSAP 64

Query: 79  FDKAMSLDWADMVDGFQSLALQSRLGIPIIYGIDAVHGSSNVYGATIFPHNVGLGATRDG 138
           F  A+S DWADM+D FQS A+QSRLGIPIIYG DAVHG++NVYGATIFPHNVGLGATRD 
Sbjct: 65  FRGALSSDWADMIDRFQSWAIQSRLGIPIIYGSDAVHGNNNVYGATIFPHNVGLGATRDA 124

Query: 139 KLVRRIGTVTALEVRASGVHYAFAPCLAVSRDPRWGRCYESYSEHTEVVRKMTSLVEGLQ 198
            LVRRIGTVTALEVRASG+HYAFAPC+AVSRDPRWGRCYESYSE TEVVRKMT LVEGLQ
Sbjct: 125 DLVRRIGTVTALEVRASGIHYAFAPCVAVSRDPRWGRCYESYSEDTEVVRKMTCLVEGLQ 184

Query: 199 GKPPEGYPKGYPFVAGRNNVIACAKHFVGDGGTDKGLNEGNTIIDSYDELERIHIAPYLD 258
           GKPP GYPKGYPFVAGRNNVIACAKHFVGDGGTDKGLNEGNTI  SYDELERIH+APYLD
Sbjct: 185 GKPPTGYPKGYPFVAGRNNVIACAKHFVGDGGTDKGLNEGNTIA-SYDELERIHMAPYLD 244

Query: 259 CIAQGLSTVMASYSSWNGNPLHTHHFLLTQVLKEKLGFKGFVISDWEALDRLSNPRGSNY 318
           CIAQG+STVMASYSSWNG PLH  HFLLTQ+LK KLGFKGFVISDW+ LDRLS PRGSNY
Sbjct: 245 CIAQGVSTVMASYSSWNGRPLHADHFLLTQILKNKLGFKGFVISDWQGLDRLSRPRGSNY 304

Query: 319 RSCICTAVNAGIDMVMVPFRYEEFIKDLLSLVESGEIPIARIDDAVERILRVKFVAGLFE 378
           R CI  AVNAGIDMVMVP RYE+FIKDLL LVESGEIP+ RIDDAVERILRVKFV+G+FE
Sbjct: 305 RLCISAAVNAGIDMVMVPLRYEQFIKDLLFLVESGEIPMTRIDDAVERILRVKFVSGVFE 364

Query: 379 HPFSDRSLIDVVGCKIHRDLAREAVRKSLVLLRNGKDPMKPFLPLDRKAKKILVAGSHAD 438
           HPFSDRSL+DVVGCKIHRDLAREAVRKSLVLL+NGKDP KPFLPLD KAKKILVAGSHAD
Sbjct: 365 HPFSDRSLLDVVGCKIHRDLAREAVRKSLVLLKNGKDPTKPFLPLDMKAKKILVAGSHAD 424

Query: 439 DLGYQCGGWTISWNGSTGRTTVGTTILDAIKEAVGDQTKVIYEQNPSAVTLNDQDISFAI 498
           DLGYQCGGWTISW+G TGR T+GTTILDAIKEAVGDQTKVIYEQNPSAVTLNDQDISFAI
Sbjct: 425 DLGYQCGGWTISWDGMTGRITIGTTILDAIKEAVGDQTKVIYEQNPSAVTLNDQDISFAI 484

Query: 499 VAIGESPYAESAGDNSKLIIPFNGNEIVKAVAGKIPTLVILISGRPLVLEPTVIENVEAL 558
           VAIGESPYAESAGDNSKLIIPFNGNEIVKAVAGKIPTLVILISGRPLVLEPTVIENVEAL
Sbjct: 485 VAIGESPYAESAGDNSKLIIPFNGNEIVKAVAGKIPTLVILISGRPLVLEPTVIENVEAL 544

Query: 559 IAAWLPGTEGNGITDVIFGDYDFTGRLPVTWFKTVEQLPVHAENNLQDSLFPFGFGLSYG 618
           IAAWLPGTEGNGITDVIFGDYDFTGRLPVTWFKTVEQLPVHAENNLQDSLFPFGFGLSYG
Sbjct: 545 IAAWLPGTEGNGITDVIFGDYDFTGRLPVTWFKTVEQLPVHAENNLQDSLFPFGFGLSYG 604

Query: 619 KEISSL 625
           KEISSL
Sbjct: 605 KEISSL 609

BLAST of Cucsa.146880 vs. NCBI nr
Match: gi|659115115|ref|XP_008457394.1| (PREDICTED: lysosomal beta glucosidase-like isoform X2 [Cucumis melo])

HSP 1 Score: 1072.8 bits (2773), Expect = 1.9e-310
Identity = 528/601 (87.85%), Postives = 561/601 (93.34%), Query Frame = 1

Query: 19  DCMYRNPGAAIEDRIKDLLSRMSLREKIGQMTQIERSVVTPSALTDLAVGSVLSGGDNPP 78
           DC+Y+N  A IE RIKDLLSRM+LREKIGQMTQIER+V TPSAL D A+GSVL+ G + P
Sbjct: 5   DCIYKNSSAPIEARIKDLLSRMTLREKIGQMTQIERTVATPSALGDFAIGSVLNSGGSAP 64

Query: 79  FDKAMSLDWADMVDGFQSLALQSRLGIPIIYGIDAVHGSSNVYGATIFPHNVGLGATRDG 138
           F  A+S DWADM+D FQ  A+QSRLGIPIIYG DAVHG++NVYGATIFPHNVGLGATRD 
Sbjct: 65  FRGALSSDWADMIDRFQYWAIQSRLGIPIIYGSDAVHGNNNVYGATIFPHNVGLGATRDA 124

Query: 139 KLVRRIGTVTALEVRASGVHYAFAPCLAVSRDPRWGRCYESYSEHTEVVRKMTSLVEGLQ 198
            LVRRIGTVTALEVRASGVHYAFAPC+AVSRDPRWGRCYESYSE TEVVRKMT LVEGLQ
Sbjct: 125 DLVRRIGTVTALEVRASGVHYAFAPCIAVSRDPRWGRCYESYSEDTEVVRKMTCLVEGLQ 184

Query: 199 GKPPEGYPKGYPFVAGRNNVIACAKHFVGDGGTDKGLNEGNTIIDSYDELERIHIAPYLD 258
           GKPP GYPKGYPFVAGRNNVIACAKHFVGDGGT+KGLNEGNTI  SYDELERIH+APYLD
Sbjct: 185 GKPPAGYPKGYPFVAGRNNVIACAKHFVGDGGTEKGLNEGNTIA-SYDELERIHMAPYLD 244

Query: 259 CIAQGLSTVMASYSSWNGNPLHTHHFLLTQVLKEKLGFKGFVISDWEALDRLSNPRGSNY 318
           CIAQG+STVMASYSSWNG PLH  HFLLTQ+LK KLGFKGFVISDW+ LDRLS PRGSNY
Sbjct: 245 CIAQGVSTVMASYSSWNGRPLHADHFLLTQILKNKLGFKGFVISDWQGLDRLSRPRGSNY 304

Query: 319 RSCICTAVNAGIDMVMVPFRYEEFIKDLLSLVESGEIPIARIDDAVERILRVKFVAGLFE 378
           R CI  AVNAGIDMVMVP RYE+FIKDLL LVESGEIP+ RIDDAVERILRVKFV+G+FE
Sbjct: 305 RLCISAAVNAGIDMVMVPLRYEQFIKDLLFLVESGEIPMTRIDDAVERILRVKFVSGVFE 364

Query: 379 HPFSDRSLIDVVGCKIHRDLAREAVRKSLVLLRNGKDPMKPFLPLDRKAKKILVAGSHAD 438
           HPFSDRSL+DVVGCKIHRDLAREAVRKSLVLL+NGKDP KPFLPLD KAKKILVAGSHAD
Sbjct: 365 HPFSDRSLLDVVGCKIHRDLAREAVRKSLVLLKNGKDPTKPFLPLDMKAKKILVAGSHAD 424

Query: 439 DLGYQCGGWTISWNGSTGRTTVGTTILDAIKEAVGDQTKVIYEQNPSAVTLNDQDISFAI 498
           DLGYQCGGWTISW+G TGR T+GTTILDAIKEAVGDQTKVIYEQNPSAVTL+DQDISFAI
Sbjct: 425 DLGYQCGGWTISWDGMTGRITIGTTILDAIKEAVGDQTKVIYEQNPSAVTLDDQDISFAI 484

Query: 499 VAIGESPYAESAGDNSKLIIPFNGNEIVKAVAGKIPTLVILISGRPLVLEPTVIENVEAL 558
           VAIGESPYAESAGD+SKLIIPFNGNEIVKAVAGKIPTLVILISGRPLVLEPTVIENVEAL
Sbjct: 485 VAIGESPYAESAGDDSKLIIPFNGNEIVKAVAGKIPTLVILISGRPLVLEPTVIENVEAL 544

Query: 559 IAAWLPGTEGNGITDVIFGDYDFTGRLPVTWFKTVEQLPVHAENNLQDSLFPFGFGLSYG 618
           +AAWLPG+EG+GITDVIFGDY+F+GRLPVTWFKTVEQLPVHAENNLQDSLFPFGFGLSYG
Sbjct: 545 VAAWLPGSEGDGITDVIFGDYNFSGRLPVTWFKTVEQLPVHAENNLQDSLFPFGFGLSYG 604

Query: 619 K 620
           K
Sbjct: 605 K 604