Cucsa.142810 (gene) Cucumber (Gy14) v1

BLAST of Cucsa.142810 vs. Swiss-Prot
Match: ASO_CUCSA (L-ascorbate oxidase OS=Cucumis sativus PE=1 SV=1)

HSP 1 Score: 846.3 bits (2185), Expect = 2.1e-244
Identity = 401/542 (73.99%), Postives = 445/542 (82.10%), Query Frame = 1

Query: 1   MFWSPDCIENIVMGINGTFPGPPIRANVNDTVVVEVINNLSTEGVVIHWHGILQRGSPWA 60
           MFWSPDC+ENIVMGING FPGP IRAN  D VVVE+ N L TEGVVIHWHGILQRG+PWA
Sbjct: 48  MFWSPDCVENIVMGINGEFPGPTIRANAGDIVVVELTNKLHTEGVVIHWHGILQRGTPWA 107

Query: 61  DGTASISQCPINPGENFTYEFKVDKPGTYFYHGHFGMQRAAGLYGSLIVDLEEGKKEPFQ 120
           DGTASISQC INPGE FTY F VDK GTYFYHGH GMQR+AGLYGSLIVD  EG+ EPF 
Sbjct: 108 DGTASISQCAINPGETFTYRFVVDKAGTYFYHGHLGMQRSAGLYGSLIVDPPEGRSEPFH 167

Query: 121 YDEEINLLLSDWWHASIHEQEVGLKSNPMRWVGEPQTILINGTGQFNCTSLDKYNTSSEH 180
           YDEEINLLLSDWWH S+H+QEVGL S PMRW+GEPQ+ILING GQF+C+   KYN   + 
Sbjct: 168 YDEEINLLLSDWWHQSVHKQEVGLSSKPMRWIGEPQSILINGKGQFDCSIAAKYNQGLKQ 227

Query: 181 CE--WKDQCAPFVLKVEPNKTYRIRIASTTSLAALNFAISNHTMLVVEADGNYVQQFVTS 240
           CE   K++CAPF+L V+P KTYRIRIASTT+LA+LNFAI NH +LVVEADGNYVQ FVTS
Sbjct: 228 CELSGKEKCAPFILHVQPKKTYRIRIASTTALASLNFAIGNHELLVVEADGNYVQPFVTS 287

Query: 241 DIDIYSGESYSVLVTTNQSTTQNYWASIGVRGREPKTSPALLLFNYLPNSVAKLPISPPP 300
           DIDIYSGESYSVL+TT+Q+  +NYW SIGVR R PKT P L L NYLPNS +KLPISPPP
Sbjct: 288 DIDIYSGESYSVLITTDQNPLENYWVSIGVRARLPKTPPGLTLLNYLPNSASKLPISPPP 347

Query: 301 EIPKWNDYDRSKNFTYRVLASAMNTTKPPQIFTRRISLLNTQNLMNGNFIKWAINNISLT 360
           E P W D+DRSKNFT+R+ A AM + KPP  + RR+ LLNTQN +NG F+KWAINN+SL 
Sbjct: 348 ETPHWEDFDRSKNFTFRIFA-AMGSPKPPVRYNRRLFLLNTQNRING-FMKWAINNVSLA 407

Query: 361 LPSTPYLGAIKFGLNSTFNQTAPPETFPKNYNIKIPPPNPNTNTGNSVYRFRSGEIVDVI 420
           LP TPYL A+K  LN+ FNQ  PPETFP NY+I  PPPNP T TGN VY+F  GE VDVI
Sbjct: 408 LPPTPYLAAMKMRLNTAFNQNPPPETFPLNYDINNPPPNPETTTGNGVYKFNMGETVDVI 467

Query: 421 LQNANALSNNTSEIHPWHLHGHDFWVLGYGEGKF-SDEDETKLNLKNPPLKNTVVIFPFG 480
           LQNAN L+ N SEIHPWHLHGHDFWVLGYGEGKF + EDE KLNLKNPPL+NTVVIFP+G
Sbjct: 468 LQNANMLNPNMSEIHPWHLHGHDFWVLGYGEGKFYAPEDEKKLNLKNPPLRNTVVIFPYG 527

Query: 481 WTAIRFVADNPGVWIFHCHIEPHLDMGMGVIFAEAVEKVREIPPYALACGRTATPQVVPI 540
           WTAIRFVADNPGVW FHCHIEPHL MGMGV+FAE V  V  IPP ALACG TA  +  P 
Sbjct: 528 WTAIRFVADNPGVWAFHCHIEPHLHMGMGVVFAEGVHMVGMIPPKALACGSTALVKNYPR 587

BLAST of Cucsa.142810 vs. Swiss-Prot
Match: ASO_CUCMA (L-ascorbate oxidase OS=Cucurbita maxima GN=AAO PE=1 SV=2)

HSP 1 Score: 824.3 bits (2128), Expect = 8.7e-238
Identity = 386/532 (72.56%), Postives = 439/532 (82.52%), Query Frame = 1

Query: 1   MFWSPDCIENIVMGINGTFPGPPIRANVNDTVVVEVINNLSTEGVVIHWHGILQRGSPWA 60
           MFW+PDC ENIVMGING FPGP IRAN  DTVVVE+IN L TEGVVIHWHGILQRG+PWA
Sbjct: 43  MFWAPDCNENIVMGINGQFPGPTIRANAGDTVVVELINKLHTEGVVIHWHGILQRGTPWA 102

Query: 61  DGTASISQCPINPGENFTYEFKVDKPGTYFYHGHFGMQRAAGLYGSLIVDLEEGKKEPFQ 120
           DGTASISQC INPGE F Y F VD PGT+FYHGH GMQR+AGLYGSLIVD  +GKKEPF 
Sbjct: 103 DGTASISQCAINPGETFFYNFTVDNPGTFFYHGHLGMQRSAGLYGSLIVDPPQGKKEPFH 162

Query: 121 YDEEINLLLSDWWHASIHEQEVGLKSNPMRWVGEPQTILINGTGQFNCTSLDKYNTSSEH 180
           YD EINLLLSDWWH SIH+QEVGL S P+RW+GEPQTIL+NG GQF+C+   KY+++ E 
Sbjct: 163 YDGEINLLLSDWWHQSIHKQEVGLSSKPIRWIGEPQTILLNGRGQFDCSIAAKYDSNLEP 222

Query: 181 CEWK--DQCAPFVLKVEPNKTYRIRIASTTSLAALNFAISNHTMLVVEADGNYVQQFVTS 240
           C+ K  + CAP++  V P KTYRIRIASTT+LAALNFAI NH +LVVEADGNYVQ F TS
Sbjct: 223 CKLKGSEPCAPYIFHVMPKKTYRIRIASTTALAALNFAIGNHPLLVVEADGNYVQPFYTS 282

Query: 241 DIDIYSGESYSVLVTTNQSTTQNYWASIGVRGREPKTSPALLLFNYLPNSVAKLPISPPP 300
           DIDIYSGESYSVL+TT+Q+ ++NYW S+G RGR P T P L L NYLPNSV+KLP SPPP
Sbjct: 283 DIDIYSGESYSVLITTDQNPSENYWVSVGTRGRHPNTPPGLTLLNYLPNSVSKLPTSPPP 342

Query: 301 EIPKWNDYDRSKNFTYRVLASAMNTTKPPQIFTRRISLLNTQNLMNGNFIKWAINNISLT 360
           E P W+D+DRSKNFTYR+ A AM + KPP    RRI LLNTQN++NG ++KWAIN++SL 
Sbjct: 343 ETPAWDDFDRSKNFTYRITA-AMGSPKPPVKSNRRIFLLNTQNVING-YVKWAINDVSLA 402

Query: 361 LPSTPYLGAIKFGLNSTFNQTAPPETFPKNYNIKIPPPNPNTNTGNSVYRFRSGEIVDVI 420
           LP TPYLGA+KF L   F+Q  PPE FP++Y+I  PP N  T  GN VY+F+ GEIVDVI
Sbjct: 403 LPPTPYLGAMKFNLLHAFDQNPPPEVFPEDYDIDTPPTNEKTKIGNGVYQFKIGEIVDVI 462

Query: 421 LQNANALSNNTSEIHPWHLHGHDFWVLGYGEGKFSDEDETKLNLKNPPLKNTVVIFPFGW 480
           LQNAN +  N SEIHPWHLHGHDFWVLGYG+GKF+ E+E+ LNLKNPPL+NTVVIFP+GW
Sbjct: 463 LQNANMMKENLSEIHPWHLHGHDFWVLGYGDGKFTAEEESSLNLKNPPLRNTVVIFPYGW 522

Query: 481 TAIRFVADNPGVWIFHCHIEPHLDMGMGVIFAEAVEKVREIPPYALACGRTA 531
           TAIRFVADNPGVW FHCHIEPHL MGMGV+FAE VEKV  IP  ALACG TA
Sbjct: 523 TAIRFVADNPGVWAFHCHIEPHLHMGMGVVFAEGVEKVGRIPTKALACGGTA 572

BLAST of Cucsa.142810 vs. Swiss-Prot
Match: ASO_CUCPM (L-ascorbate oxidase OS=Cucurbita pepo var. melopepo PE=1 SV=1)

HSP 1 Score: 817.4 bits (2110), Expect = 1.1e-235
Identity = 380/532 (71.43%), Postives = 437/532 (82.14%), Query Frame = 1

Query: 1   MFWSPDCIENIVMGINGTFPGPPIRANVNDTVVVEVINNLSTEGVVIHWHGILQRGSPWA 60
           MFW+P+C ENIVMGING FPGP IRAN  D+VVVE+ N L TEGVVIHWHGILQRG+PWA
Sbjct: 13  MFWAPNCNENIVMGINGQFPGPTIRANAGDSVVVELTNKLHTEGVVIHWHGILQRGTPWA 72

Query: 61  DGTASISQCPINPGENFTYEFKVDKPGTYFYHGHFGMQRAAGLYGSLIVDLEEGKKEPFQ 120
           DGTASISQC INPGE F Y F VD PGT+FYHGH GMQR+AGLYGSLIVD  +GKKEPF 
Sbjct: 73  DGTASISQCAINPGETFFYNFTVDNPGTFFYHGHLGMQRSAGLYGSLIVDPPQGKKEPFH 132

Query: 121 YDEEINLLLSDWWHASIHEQEVGLKSNPMRWVGEPQTILINGTGQFNCTSLDKYNTSSEH 180
           YD EINLLLSDWWH SIH+QEVGL S P+RW+GEPQTIL+NG GQF+C+   KY+++ E 
Sbjct: 133 YDGEINLLLSDWWHQSIHKQEVGLSSKPIRWIGEPQTILLNGRGQFDCSIAAKYDSNLEP 192

Query: 181 CEWK--DQCAPFVLKVEPNKTYRIRIASTTSLAALNFAISNHTMLVVEADGNYVQQFVTS 240
           C+ K  + CAP++  V P KTYRIRIASTT+LAALNFAI NH +LVVEADGNYVQ F TS
Sbjct: 193 CKLKGSESCAPYIFHVSPKKTYRIRIASTTALAALNFAIGNHQLLVVEADGNYVQPFYTS 252

Query: 241 DIDIYSGESYSVLVTTNQSTTQNYWASIGVRGREPKTSPALLLFNYLPNSVAKLPISPPP 300
           DIDIYSGESYSVL+TT+Q+ ++NYW S+G R R P T P L L NYLPNSV+KLP SPPP
Sbjct: 253 DIDIYSGESYSVLITTDQNPSENYWVSVGTRARHPNTPPGLTLLNYLPNSVSKLPTSPPP 312

Query: 301 EIPKWNDYDRSKNFTYRVLASAMNTTKPPQIFTRRISLLNTQNLMNGNFIKWAINNISLT 360
           + P W+D+DRSKNFTYR+ A AM + KPP  F RRI LLNTQN++NG ++KWAIN++SL 
Sbjct: 313 QTPAWDDFDRSKNFTYRITA-AMGSPKPPVKFNRRIFLLNTQNVING-YVKWAINDVSLA 372

Query: 361 LPSTPYLGAIKFGLNSTFNQTAPPETFPKNYNIKIPPPNPNTNTGNSVYRFRSGEIVDVI 420
           LP TPYLGA+K+ L   F+Q  PPE FP++Y+I  PP N  T  GN VY+F+ GE+VDVI
Sbjct: 373 LPPTPYLGAMKYNLLHAFDQNPPPEVFPEDYDIDTPPTNEKTRIGNGVYQFKIGEVVDVI 432

Query: 421 LQNANALSNNTSEIHPWHLHGHDFWVLGYGEGKFSDEDETKLNLKNPPLKNTVVIFPFGW 480
           LQNAN +  N SE HPWHLHGHDFWVLGYG+GKFS E+E+ LNLKNPPL+NTVVIFP+GW
Sbjct: 433 LQNANMMKENLSETHPWHLHGHDFWVLGYGDGKFSAEEESSLNLKNPPLRNTVVIFPYGW 492

Query: 481 TAIRFVADNPGVWIFHCHIEPHLDMGMGVIFAEAVEKVREIPPYALACGRTA 531
           TAIRFVADNPGVW FHCHIEPHL MGMGV+FAE VEKV  IP  ALACG TA
Sbjct: 493 TAIRFVADNPGVWAFHCHIEPHLHMGMGVVFAEGVEKVGRIPTKALACGGTA 542

BLAST of Cucsa.142810 vs. Swiss-Prot
Match: ASO_TOBAC (L-ascorbate oxidase OS=Nicotiana tabacum GN=AAO PE=2 SV=1)

HSP 1 Score: 719.2 bits (1855), Expect = 4.0e-206
Identity = 339/530 (63.96%), Postives = 406/530 (76.60%), Query Frame = 1

Query: 3   WSPDCIENIVMGINGTFPGPPIRANVNDTVVVEVINNLSTEGVVIHWHGILQRGSPWADG 62
           WSPD  E++VMGING FPGP IRA   DTV V + N L TEGVVIHWHGI Q G+PWADG
Sbjct: 42  WSPDGEESVVMGINGQFPGPTIRAKAGDTVAVHLTNKLHTEGVVIHWHGIRQIGTPWADG 101

Query: 63  TASISQCPINPGENFTYEFKVDKPGTYFYHGHFGMQRAAGLYGSLIVDLEEGKKEPFQYD 122
           TA+ISQC INPGE F Y FKVDK GTYFYHGH+GMQR+AGLYGSLIV++ EG+KEPF YD
Sbjct: 102 TAAISQCAINPGETFLYRFKVDKAGTYFYHGHYGMQRSAGLYGSLIVEVGEGEKEPFHYD 161

Query: 123 EEINLLLSDWWHASIHEQEVGLKSNPMRWVGEPQTILINGTGQFNCTSLDKYNTSS-EHC 182
            E NLLLSDWWH   HEQEV L SNP+RW+GEPQT+L+NG GQ+NC+   +++      C
Sbjct: 162 GEFNLLLSDWWHKGSHEQEVDLSSNPLRWIGEPQTLLLNGRGQYNCSLAARFSKPPLPQC 221

Query: 183 EWK--DQCAPFVLKVEPNKTYRIRIASTTSLAALNFAISNHTMLVVEADGNYVQQFVTSD 242
           + +  +Q AP +L+V PNK YR+R+ASTT+L +L+ AI  H M+VVEADGNYVQ F   D
Sbjct: 222 KLRGGEQYAPQILRVRPNKIYRLRVASTTALGSLSLAIGGHKMVVVEADGNYVQPFSVQD 281

Query: 243 IDIYSGESYSVLVTTNQSTTQNYWASIGVRGREPKTSPALLLFNYLPNSVAKLPISPPPE 302
           +DIYSGESYSVL  T+Q  T+NYW SI VRGREPKT   L L NYLPNS +K P  PPP 
Sbjct: 282 MDIYSGESYSVLFKTDQDPTKNYWISINVRGREPKTPQGLTLLNYLPNSASKFPTLPPPI 341

Query: 303 IPKWNDYDRSKNFTYRVLASAMNTTKPPQIFTRRISLLNTQNLMNGNFIKWAINNISLTL 362
            P WNDY+ SK+F+ ++ A  M + KPP    RRI LLNTQN ++G + KWAINN+SL L
Sbjct: 342 APLWNDYNHSKSFSNKIFA-LMGSPKPPPQNHRRIILLNTQNKIDG-YTKWAINNVSLVL 401

Query: 363 PSTPYLGAIKFGLNSTFNQTAPPETFPKNYNIKIPPPNPNTNTGNSVYRFRSGEIVDVIL 422
           P+  YLG+I++G+N+ F+   PP+ FPK+Y++    PN N+  GN VY  +    +D+IL
Sbjct: 402 PTQLYLGSIRYGINA-FDTKPPPDNFPKDYDVLKQAPNSNSTYGNGVYMLKFNTTIDIIL 461

Query: 423 QNANALSNNTSEIHPWHLHGHDFWVLGYGEGKFSDEDETKLNLKNPPLKNTVVIFPFGWT 482
           QNANAL+ + SEIHPWHLHGHDFWVLGYGEGKFS++D  K NLKNPPL+NT VIFPFGWT
Sbjct: 462 QNANALAKDVSEIHPWHLHGHDFWVLGYGEGKFSEKDVKKFNLKNPPLRNTAVIFPFGWT 521

Query: 483 AIRFVADNPGVWIFHCHIEPHLDMGMGVIFAEAVEKVREIPPYALACGRT 530
           A+RFV DNPGVW FHCHIEPHL MGMGVIFAE V  V++IP  ALACG T
Sbjct: 522 ALRFVTDNPGVWAFHCHIEPHLHMGMGVIFAEGVHLVKKIPKEALACGLT 568

BLAST of Cucsa.142810 vs. Swiss-Prot
Match: LAC11_ORYSJ (Putative laccase-11 OS=Oryza sativa subsp. japonica GN=LAC11 PE=5 SV=2)

HSP 1 Score: 261.9 bits (668), Expect = 1.7e-68
Identity = 183/529 (34.59%), Postives = 271/529 (51.23%), Query Frame = 1

Query: 7   CIENIVMGINGTFPGPPIRANVNDTVVVEVINNLSTEGVVIHWHGILQRGSPWADGTASI 66
           C+   V  +NG FPGP +     DT+V+ V NN++   V  HWHGI Q  S WADG A I
Sbjct: 8   CVTKSVPTVNGQFPGPKLVVREGDTLVIRVTNNINNN-VTFHWHGIRQVRSGWADGPAYI 67

Query: 67  SQCPINPGENFTYEFKVD-KPGTYFYHGHFGMQRAAGLYGSLIVDLEEGKKEPF-QYDEE 126
           +QCPI  G ++ Y F V  + GT ++H HF   RA  LYG L++    G   PF +   E
Sbjct: 68  TQCPIRSGGSYVYRFTVTGQRGTLWWHAHFSWLRAT-LYGPLVILPPRGVAYPFPKPHRE 127

Query: 127 INLLLSDWWHASIHEQEVGLKSNPMRWVGEPQTILINGTGQFNCTSLDKYNTSSEHCEWK 186
           + LLL +W++A   + E  +K   ++  G P    ++    FN      YN SS +  +K
Sbjct: 128 VPLLLGEWFNA---DPEAVIKQ-ALQTGGGPN---VSDAYTFNGLPGPTYNCSSSNDTFK 187

Query: 187 DQCAPFVLKVEPNKTYRIRIASTTSLAALNFAISNHTMLVVEADGNYVQQFVTSDIDIYS 246
                  L+V P KTY +R+ +      L F ++NHT++VV+AD +YV+ F  + + I  
Sbjct: 188 -------LRVRPGKTYLLRLINAALNDELFFGVANHTLMVVQADASYVKPFAATALVISP 247

Query: 247 GESYSVLVTT--NQSTTQNYWASIG-----VRGREPKTSPALLLFNYLPNSVAKLPISPP 306
           G++  VL+T   N   ++++  ++      V   +  T+ A+L +     S A L   P 
Sbjct: 248 GQTMDVLLTAAANNPPSRSFAIAVAPYTNTVGTFDNTTAVAVLEYYGAATSAAALRSLPL 307

Query: 307 PEIPKWNDYDRSKNFT--YRVLASAMNTTKPPQIFTRRISLL------NTQNLMNG---- 366
           P +P +ND     NF+  +R LASA    + P+   R             Q+ +NG    
Sbjct: 308 PSLPAYNDTGAVANFSASFRSLASAQYPARVPRTVDRHFFFAVGLGADPCQSPVNGTCQG 367

Query: 367 -NFIKWA--INNISLTLPSTPYLGAI---KFGLNSTFNQTAPPETFPKNYNIKIPPPNPN 426
            N  ++A  +NN+S  +P T  L A    ++      N  A P T P NY    PP N  
Sbjct: 368 PNNTRFAASMNNVSFVMPRTSLLQAHYQRRYNGVLAANFPAAPRT-PFNYT-GTPPNNTF 427

Query: 427 TNTGNSVYRFRSGEIVDVILQNANALSNNTSEIHPWHLHGHDFWVLGYGEGKFSDEDET- 486
              G  V        V+V+LQ+ + L    +E HP HLHG+DF+V+G G G +   ++T 
Sbjct: 428 VTHGTRVVPLSFNTTVEVVLQDTSILG---AESHPLHLHGYDFYVVGTGFGNYDASNDTA 487

Query: 487 KLNLKNPPLKNTVVIFPFGWTAIRFVADNPGVWIFHCHIEPHLDMGMGV 508
           K NL +P  +NT+ +   GW AIRFVADNPGVWI HCH++ HL  G+ +
Sbjct: 488 KYNLVDPVQRNTISVPTAGWVAIRFVADNPGVWIMHCHLDVHLSWGLSM 515

BLAST of Cucsa.142810 vs. TrEMBL
Match: A0A0A0M2J6_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_1G602530 PE=3 SV=1)

HSP 1 Score: 1275.8 bits (3300), Expect = 0.0e+00
Identity = 609/625 (97.44%), Postives = 612/625 (97.92%), Query Frame = 1

Query: 1   MFWSPDCIENIVMGINGTFPGPPIRANVNDTVVVEVINNLSTEGVVIHWHGILQRGSPWA 60
           MFWSPDCIENIVMGINGTFPGPPIRANVNDTVVVEVINNLSTEGVVIHWHGILQRGSPWA
Sbjct: 49  MFWSPDCIENIVMGINGTFPGPPIRANVNDTVVVEVINNLSTEGVVIHWHGILQRGSPWA 108

Query: 61  DGTASISQCPINPGENFTYEFKVDKPGTYFYHGHFGMQRAAGLYGSLIVDLEEGKKEPFQ 120
           DGTASISQCPINPGENFTYEFKVDKPGTYFYHGHFGMQRAAGLYGSLIVDLEEGKKEPFQ
Sbjct: 109 DGTASISQCPINPGENFTYEFKVDKPGTYFYHGHFGMQRAAGLYGSLIVDLEEGKKEPFQ 168

Query: 121 YDEEINLLLSDWWHASIHEQEVGLKSNPMRWVGEPQTILINGTGQFNCTSLDKYNTSSEH 180
           YDEEINLLLSDWWHAS+HEQEVGLKSNPMRWVGEPQTILINGTGQFNCTSL+KYNTSSEH
Sbjct: 169 YDEEINLLLSDWWHASVHEQEVGLKSNPMRWVGEPQTILINGTGQFNCTSLEKYNTSSEH 228

Query: 181 CEWKDQCAPFVLKVEPNKTYRIRIASTTSLAALNFAIS-----------NHTMLVVEADG 240
           CEWKDQCAPFVLKVEPNKTYRIRIASTTSLAALNFAIS           NHTMLVVEADG
Sbjct: 229 CEWKDQCAPFVLKVEPNKTYRIRIASTTSLAALNFAISKKFHHFSFLFQNHTMLVVEADG 288

Query: 241 NYVQQFVTSDIDIYSGESYSVLVTTNQSTTQNYWASIGVRGREPKTSPALLLFNYLPNSV 300
           NYVQQFVTSDIDIYSGESYSVLVTTNQSTTQNYWASIGVRGREPKTSPALLLFNYLPNSV
Sbjct: 289 NYVQQFVTSDIDIYSGESYSVLVTTNQSTTQNYWASIGVRGREPKTSPALLLFNYLPNSV 348

Query: 301 AKLPISPPPEIPKWNDYDRSKNFTYRVLASAMNTTKPPQIFTRRISLLNTQNLMNGNFIK 360
           AKLPISPPPEIPKWNDYDRSKNFTYRVLASAMNTTKPP IFTRRISLLNTQNLMNGNFIK
Sbjct: 349 AKLPISPPPEIPKWNDYDRSKNFTYRVLASAMNTTKPPPIFTRRISLLNTQNLMNGNFIK 408

Query: 361 WAINNISLTLPSTPYLGAIKFGLNSTFNQTAPPETFPKNYNIKIPPPNPNTNTGNSVYRF 420
           WAINNISLTLPSTPYLGAIKFGLNSTFNQTAPPETFPKNYNIKIPPPNPNTNTGNSVYRF
Sbjct: 409 WAINNISLTLPSTPYLGAIKFGLNSTFNQTAPPETFPKNYNIKIPPPNPNTNTGNSVYRF 468

Query: 421 RSGEIVDVILQNANALSNNTSEIHPWHLHGHDFWVLGYGEGKFSDEDETKLNLKNPPLKN 480
           RSGEIVDVILQNANALSNNTSEIHPWHLHGHDFWVLGYGEGKFSDEDETKLNLKNPPLKN
Sbjct: 469 RSGEIVDVILQNANALSNNTSEIHPWHLHGHDFWVLGYGEGKFSDEDETKLNLKNPPLKN 528

Query: 481 TVVIFPFGWTAIRFVADNPGVWIFHCHIEPHLDMGMGVIFAEAVEKVREIPPYALACGRT 540
           TVVIFPFGWTAIRFVADNPGVWIFHCHIEPHLDMGMGVIFAEAVEKVREIPPYALACGRT
Sbjct: 529 TVVIFPFGWTAIRFVADNPGVWIFHCHIEPHLDMGMGVIFAEAVEKVREIPPYALACGRT 588

Query: 541 ATPQVVPIIPRPNPPGLDNPYYIIWIGLVIFLCVIGLCYVCLYRIRFNHLSWRPYLGISE 600
           ATPQVVPIIPRPNPPGLDNPYYIIWIGLVIFLCVIGLCYVCLYRIRFN+LSWRPYLGISE
Sbjct: 589 ATPQVVPIIPRPNPPGLDNPYYIIWIGLVIFLCVIGLCYVCLYRIRFNNLSWRPYLGISE 648

Query: 601 SQSNQIEFSDLSQSNPNSNPTHEEP 615
           SQSNQIEFSDLSQSNPN NPTHEEP
Sbjct: 649 SQSNQIEFSDLSQSNPNPNPTHEEP 673

BLAST of Cucsa.142810 vs. TrEMBL
Match: Q9ZR59_CUCME (L-ascorbate oxidase OS=Cucumis melo GN=ao3 PE=3 SV=1)

HSP 1 Score: 1152.9 bits (2981), Expect = 0.0e+00
Identity = 557/626 (88.98%), Postives = 581/626 (92.81%), Query Frame = 1

Query: 1   MFWSPDCIENIVMGINGTFPGPPIRANVNDTVVVEVINNLSTEGVVIHWHGILQRGSPWA 60
           MFWSPDC+ENIV+GINGTFPGPPIRANVNDTVVVEVINNLSTEGVVIHWHGILQRG+PWA
Sbjct: 47  MFWSPDCVENIVLGINGTFPGPPIRANVNDTVVVEVINNLSTEGVVIHWHGILQRGTPWA 106

Query: 61  DGTASISQCPINPGENFTYEFKVDKPGTYFYHGHFGMQRAAGLYGSLIVDLEEGKKEPFQ 120
           DGTASISQCPINPGENFTYEFKVDKPGTYFYHGHFGMQRAAGLYGSLIVDLEEGKKEPFQ
Sbjct: 107 DGTASISQCPINPGENFTYEFKVDKPGTYFYHGHFGMQRAAGLYGSLIVDLEEGKKEPFQ 166

Query: 121 YDEEINLLLSDWWHASIHEQEVGLKSNPMRWVGEPQTILINGTGQFNCTSLDKYNTSSEH 180
           YDEEINLLLSDWWH SI EQEVGLKSNPMRW+GE Q ILINGTGQFNCTSLDKYNTSSEH
Sbjct: 167 YDEEINLLLSDWWHTSIQEQEVGLKSNPMRWIGELQVILINGTGQFNCTSLDKYNTSSEH 226

Query: 181 CEWKDQCAPFVLKVEPNKTYRIRIASTTSLAALNFAISNHTMLVVEADGNYVQQFVTSDI 240
           CEWKDQCAPFVLKVEPNKTYRIRIASTTSL+ALNFAI+NHTMLVVEADGNYV  F TSDI
Sbjct: 227 CEWKDQCAPFVLKVEPNKTYRIRIASTTSLSALNFAIANHTMLVVEADGNYVDPFETSDI 286

Query: 241 DIYSGESYSVLVTTNQSTTQNYWASIGVRGREPKTSPALLLFNYLPNSVAKLPISPPPEI 300
           DIYSGESYSVLVTTNQ+TTQNYWASIGVRGR+PKTSPALLLFNYLPNSVAKLPISPPPE 
Sbjct: 287 DIYSGESYSVLVTTNQATTQNYWASIGVRGRKPKTSPALLLFNYLPNSVAKLPISPPPET 346

Query: 301 PKWNDYDRSKNFTYRVLASAMNTTKPPQIFTRRISLLNTQNLMNGNFIKWAINNISLTLP 360
           PKW+DYDRSK+FT RVLASAMNTTKPP IFTRRISLLNTQN +NG F KWAINNISLTLP
Sbjct: 347 PKWDDYDRSKSFTNRVLASAMNTTKPPPIFTRRISLLNTQNRINGKFTKWAINNISLTLP 406

Query: 361 STPYLGAIKFGLNSTFNQTAPPETFPKNYNIKIPPPNPNTNTGNSVYRFRSGEIVDVILQ 420
           STPYLGAIKFGLNSTFNQT PPETFPKNYNIKIPPPNPNTNTGN VYRFRSGEIVDVILQ
Sbjct: 407 STPYLGAIKFGLNSTFNQTPPPETFPKNYNIKIPPPNPNTNTGNGVYRFRSGEIVDVILQ 466

Query: 421 NANALSNNTSEIHPWHLHGHDFWVLGYGEGKFSDEDETKLNLKNPPLKNTVVIFPFGWTA 480
           NANAL+NNTSEIHPWHLHGHDFWVLG+G+ KFS  DETKLNLKNPPLKNTVVIFPFGWTA
Sbjct: 467 NANALTNNTSEIHPWHLHGHDFWVLGFGDFKFSAADETKLNLKNPPLKNTVVIFPFGWTA 526

Query: 481 IRFVADNPGVWIFHCHIEPHLDMGMGVIFAEAVEKVREIPPYALACGRTATPQVVPIIPR 540
           IRFVADNPGVWIFHCHIEPHLDMGMGVIFAEAVE+VREIPPYALACGRTAT  VVPIIP+
Sbjct: 527 IRFVADNPGVWIFHCHIEPHLDMGMGVIFAEAVERVREIPPYALACGRTATHNVVPIIPQ 586

Query: 541 PNP---PGLDNPYYIIW-IGLVIFLCVIGLCYVCLYRI------RFNHLSWRPYLGISES 600
           P P   PG   PY+++W IGLVI +C IGL  VCLYRI       FN+LSWRPYLG+S+S
Sbjct: 587 PKPPRGPGPVIPYFMLWVIGLVIIVCGIGLFGVCLYRIGLRRGYTFNNLSWRPYLGLSDS 646

Query: 601 QSNQIEFSDLSQSN---PNSNPTHEE 614
           QSNQIEFSDLS+SN     SNPT EE
Sbjct: 647 QSNQIEFSDLSRSNLTQEESNPTQEE 672

BLAST of Cucsa.142810 vs. TrEMBL
Match: Q9M5B5_CUCME (Ascorbate oxidase AO1 OS=Cucumis melo PE=2 SV=1)

HSP 1 Score: 867.1 bits (2239), Expect = 1.3e-248
Identity = 411/533 (77.11%), Postives = 458/533 (85.93%), Query Frame = 1

Query: 1   MFWSPDCIENIVMGINGTFPGPPIRANVNDTVVVEVINNLSTEGVVIHWHGILQRGSPWA 60
           MF SPDC EN+VMGIN  FPGP IRANV DTVVVE+IN LSTE VVIHWHGILQ+G+PWA
Sbjct: 49  MFRSPDCKENLVMGINHQFPGPTIRANVGDTVVVELINKLSTEDVVIHWHGILQKGTPWA 108

Query: 61  DGTASISQCPINPGENFTYEFKVDKPGTYFYHGHFGMQRAAGLYGSLIVDLEEGKKEPFQ 120
           DGTASISQC INPGE+FTY+F VDKPGTYFYHGH GMQRAAGLYGSLIVDLEEGKKEPF 
Sbjct: 109 DGTASISQCAINPGESFTYQFVVDKPGTYFYHGHLGMQRAAGLYGSLIVDLEEGKKEPFH 168

Query: 121 YDEEINLLLSDWWHASIHEQEVGLKSNPMRWVGEPQTILINGTGQFNCTSLDKYNTSSEH 180
           YDEEINLLLSDWWH S+ +QEVGL S+PMRW+GEPQ+ILING GQ NC+   KY  + E 
Sbjct: 169 YDEEINLLLSDWWHESVLKQEVGLSSSPMRWIGEPQSILINGRGQSNCSIGAKYMKNMEQ 228

Query: 181 CE--WKDQCAPFVLKVEPNKTYRIRIASTTSLAALNFAISNHTMLVVEADGNYVQQFVTS 240
           C+    ++CAPF+LKV+PNKTYRIRI STTSL+ALNFAI NH +LVVEADGNYVQ F+TS
Sbjct: 229 CKLSGSEECAPFILKVKPNKTYRIRIISTTSLSALNFAIGNHKLLVVEADGNYVQPFLTS 288

Query: 241 DIDIYSGESYSVLVTTNQSTTQNYWASIGVRGREPKTSPALLLFNYLPNSVAKLPISPPP 300
           DIDIYSGESYSVL+ T+Q+ +QNYW S+GVRGREPKT P L L NYLPNS +KLPISPPP
Sbjct: 289 DIDIYSGESYSVLINTDQNPSQNYWVSLGVRGREPKTPPGLTLLNYLPNSFSKLPISPPP 348

Query: 301 EIPKWNDYDRSKNFTYRVLASAMNTTKPPQIFTRRISLLNTQNLMNGNFIKWAINNISLT 360
           E PKWNDYDRSKNFTYR+LA AM + KPP   TRRI LLNTQN MNG + KWAINN+SLT
Sbjct: 349 ETPKWNDYDRSKNFTYRILA-AMGSPKPPVKSTRRIFLLNTQNKMNGGYTKWAINNVSLT 408

Query: 361 LPSTPYLGAIKFGLNSTFNQTAPPETFPKNYNIKIPPPNPNTNTGNSVYRFRSGEIVDVI 420
           LPSTPYLGAIK G+N TF+Q  PPET+P+NY+I  PPPN NT TGN VYRF+ GEIVDV+
Sbjct: 409 LPSTPYLGAIKIGINDTFDQNPPPETYPENYDIHKPPPNGNTITGNGVYRFQLGEIVDVV 468

Query: 421 LQNANALSNNTSEIHPWHLHGHDFWVLGYGEGKFSDE-DETKLNLKNPPLKNTVVIFPFG 480
           LQNAN L++  SEIHPWHLHGHDFWVLGYGEGKFS   DETKLNLK+PPL+NTVVIFPFG
Sbjct: 469 LQNANTLNDKHSEIHPWHLHGHDFWVLGYGEGKFSAAVDETKLNLKDPPLRNTVVIFPFG 528

Query: 481 WTAIRFVADNPGVWIFHCHIEPHLDMGMGVIFAEAVEKVREIPPYALACGRTA 531
           WT +RFVADNPGVW FHCHIEPHL MGMGV+FAE V+KV  IP +A+ACG TA
Sbjct: 529 WTVLRFVADNPGVWAFHCHIEPHLHMGMGVVFAEGVDKVGRIPTHAMACGGTA 580

BLAST of Cucsa.142810 vs. TrEMBL
Match: E7BBM8_CUCSA (Ascorbate oxidase OS=Cucumis sativus GN=aso PE=2 SV=1)

HSP 1 Score: 846.7 bits (2186), Expect = 1.8e-242
Identity = 401/542 (73.99%), Postives = 446/542 (82.29%), Query Frame = 1

Query: 1   MFWSPDCIENIVMGINGTFPGPPIRANVNDTVVVEVINNLSTEGVVIHWHGILQRGSPWA 60
           MFWSPDC+ENIVMGING FPGP IRAN  D VVVE+ N L TEGVVIHWHGILQRG+PWA
Sbjct: 48  MFWSPDCVENIVMGINGEFPGPTIRANAGDIVVVELTNKLHTEGVVIHWHGILQRGTPWA 107

Query: 61  DGTASISQCPINPGENFTYEFKVDKPGTYFYHGHFGMQRAAGLYGSLIVDLEEGKKEPFQ 120
           DGTASISQC INPGE FTY F VDK GTYFYHGH GMQR+AGLYGSLIVD  EG+ EPF 
Sbjct: 108 DGTASISQCAINPGETFTYRFVVDKAGTYFYHGHLGMQRSAGLYGSLIVDPPEGRSEPFH 167

Query: 121 YDEEINLLLSDWWHASIHEQEVGLKSNPMRWVGEPQTILINGTGQFNCTSLDKYNTSSEH 180
           YDEEINLLLSDWWH S+H+QEVGL S PMRW+GEPQ+ILING GQF+C+   KYN   + 
Sbjct: 168 YDEEINLLLSDWWHQSVHKQEVGLSSKPMRWIGEPQSILINGKGQFDCSIAAKYNQGLKQ 227

Query: 181 CE--WKDQCAPFVLKVEPNKTYRIRIASTTSLAALNFAISNHTMLVVEADGNYVQQFVTS 240
           CE   K++CAPF+L V+P KTYRIRIASTT+LA+LNFAI NH +LVVEADGNYVQ FVTS
Sbjct: 228 CELSGKEKCAPFILHVQPKKTYRIRIASTTALASLNFAIGNHELLVVEADGNYVQPFVTS 287

Query: 241 DIDIYSGESYSVLVTTNQSTTQNYWASIGVRGREPKTSPALLLFNYLPNSVAKLPISPPP 300
           DIDIYSGESYSVL+TT+Q+  +NYW SIGVR R PKT P L L NYLPNS +KLPISPPP
Sbjct: 288 DIDIYSGESYSVLITTDQNPLENYWVSIGVRARLPKTPPGLTLLNYLPNSASKLPISPPP 347

Query: 301 EIPKWNDYDRSKNFTYRVLASAMNTTKPPQIFTRRISLLNTQNLMNGNFIKWAINNISLT 360
           E P W D+DRSKNFT+R+ A AM + KPP  + RR+ LLNTQN +NG F+KWAINN+SL 
Sbjct: 348 ETPHWEDFDRSKNFTFRIFA-AMGSPKPPVRYNRRLFLLNTQNRING-FMKWAINNVSLA 407

Query: 361 LPSTPYLGAIKFGLNSTFNQTAPPETFPKNYNIKIPPPNPNTNTGNSVYRFRSGEIVDVI 420
           LP TPYL A+K  LN+ FNQ+ PPETFP NY+I  PPPNP T TGN VY+F  GE VDVI
Sbjct: 408 LPPTPYLAAMKMRLNTAFNQSPPPETFPLNYDINNPPPNPETTTGNGVYKFNMGETVDVI 467

Query: 421 LQNANALSNNTSEIHPWHLHGHDFWVLGYGEGKF-SDEDETKLNLKNPPLKNTVVIFPFG 480
           LQNAN L+ N SEIHPWHLHGHDFWVLGYGEGKF + EDE KLNLKNPPL+NTVVIFP+G
Sbjct: 468 LQNANMLNPNMSEIHPWHLHGHDFWVLGYGEGKFYAPEDEKKLNLKNPPLRNTVVIFPYG 527

Query: 481 WTAIRFVADNPGVWIFHCHIEPHLDMGMGVIFAEAVEKVREIPPYALACGRTATPQVVPI 540
           WTAIRFVADNPGVW FHCHIEPHL MGMGV+FAE V  V  IPP ALACG TA  +  P 
Sbjct: 528 WTAIRFVADNPGVWAFHCHIEPHLHMGMGVVFAEGVHMVGMIPPKALACGSTALVKNYPR 587

BLAST of Cucsa.142810 vs. TrEMBL
Match: A0A0A0LXB9_CUCSA (L-ascorbate oxidase OS=Cucumis sativus GN=Csa_1G604040 PE=3 SV=1)

HSP 1 Score: 846.3 bits (2185), Expect = 2.4e-242
Identity = 401/542 (73.99%), Postives = 445/542 (82.10%), Query Frame = 1

Query: 1   MFWSPDCIENIVMGINGTFPGPPIRANVNDTVVVEVINNLSTEGVVIHWHGILQRGSPWA 60
           MFWSPDC+ENIVMGING FPGP IRAN  D VVVE+ N L TEGVVIHWHGILQRG+PWA
Sbjct: 48  MFWSPDCVENIVMGINGEFPGPTIRANAGDIVVVELTNKLHTEGVVIHWHGILQRGTPWA 107

Query: 61  DGTASISQCPINPGENFTYEFKVDKPGTYFYHGHFGMQRAAGLYGSLIVDLEEGKKEPFQ 120
           DGTASISQC INPGE FTY F VDK GTYFYHGH GMQR+AGLYGSLIVD  EG+ EPF 
Sbjct: 108 DGTASISQCAINPGETFTYRFVVDKAGTYFYHGHLGMQRSAGLYGSLIVDPPEGRSEPFH 167

Query: 121 YDEEINLLLSDWWHASIHEQEVGLKSNPMRWVGEPQTILINGTGQFNCTSLDKYNTSSEH 180
           YDEEINLLLSDWWH S+H+QEVGL S PMRW+GEPQ+ILING GQF+C+   KYN   + 
Sbjct: 168 YDEEINLLLSDWWHQSVHKQEVGLSSKPMRWIGEPQSILINGKGQFDCSIAAKYNQGLKQ 227

Query: 181 CE--WKDQCAPFVLKVEPNKTYRIRIASTTSLAALNFAISNHTMLVVEADGNYVQQFVTS 240
           CE   K++CAPF+L V+P KTYRIRIASTT+LA+LNFAI NH +LVVEADGNYVQ FVTS
Sbjct: 228 CELSGKEKCAPFILHVQPKKTYRIRIASTTALASLNFAIGNHELLVVEADGNYVQPFVTS 287

Query: 241 DIDIYSGESYSVLVTTNQSTTQNYWASIGVRGREPKTSPALLLFNYLPNSVAKLPISPPP 300
           DIDIYSGESYSVL+TT+Q+  +NYW SIGVR R PKT P L L NYLPNS +KLPISPPP
Sbjct: 288 DIDIYSGESYSVLITTDQNPLENYWVSIGVRARLPKTPPGLTLLNYLPNSASKLPISPPP 347

Query: 301 EIPKWNDYDRSKNFTYRVLASAMNTTKPPQIFTRRISLLNTQNLMNGNFIKWAINNISLT 360
           E P W D+DRSKNFT+R+ A AM + KPP  + RR+ LLNTQN +NG F+KWAINN+SL 
Sbjct: 348 ETPHWEDFDRSKNFTFRIFA-AMGSPKPPVRYNRRLFLLNTQNRING-FMKWAINNVSLA 407

Query: 361 LPSTPYLGAIKFGLNSTFNQTAPPETFPKNYNIKIPPPNPNTNTGNSVYRFRSGEIVDVI 420
           LP TPYL A+K  LN+ FNQ  PPETFP NY+I  PPPNP T TGN VY+F  GE VDVI
Sbjct: 408 LPPTPYLAAMKMRLNTAFNQNPPPETFPLNYDINNPPPNPETTTGNGVYKFNMGETVDVI 467

Query: 421 LQNANALSNNTSEIHPWHLHGHDFWVLGYGEGKF-SDEDETKLNLKNPPLKNTVVIFPFG 480
           LQNAN L+ N SEIHPWHLHGHDFWVLGYGEGKF + EDE KLNLKNPPL+NTVVIFP+G
Sbjct: 468 LQNANMLNPNMSEIHPWHLHGHDFWVLGYGEGKFYAPEDEKKLNLKNPPLRNTVVIFPYG 527

Query: 481 WTAIRFVADNPGVWIFHCHIEPHLDMGMGVIFAEAVEKVREIPPYALACGRTATPQVVPI 540
           WTAIRFVADNPGVW FHCHIEPHL MGMGV+FAE V  V  IPP ALACG TA  +  P 
Sbjct: 528 WTAIRFVADNPGVWAFHCHIEPHLHMGMGVVFAEGVHMVGMIPPKALACGSTALVKNYPR 587

BLAST of Cucsa.142810 vs. TAIR10
Match: AT5G21105.1 (AT5G21105.1 Plant L-ascorbate oxidase)

HSP 1 Score: 713.0 bits (1839), Expect = 1.6e-205
Identity = 327/533 (61.35%), Postives = 410/533 (76.92%), Query Frame = 1

Query: 2   FWSPDCIENIVMGINGTFPGPPIRANVNDTVVVEVINNLSTEGVVIHWHGILQRGSPWAD 61
           +WSPDC E  VM +NG FPGP I+A   DT+VV + N L+TEG+VIHWHGI Q GSPWAD
Sbjct: 48  YWSPDCKEGAVMTVNGEFPGPTIKAFAGDTIVVNLTNKLTTEGLVIHWHGIRQFGSPWAD 107

Query: 62  GTASISQCPINPGENFTYEFKVDKPGTYFYHGHFGMQRAAGLYGSLIVDLEEGKKEPFQY 121
           G A ++QC INPGE FTY F V+KPGT+FYHGH+GMQR+AGLYGSLIVD+ +GK E  +Y
Sbjct: 108 GAAGVTQCAINPGETFTYNFTVEKPGTHFYHGHYGMQRSAGLYGSLIVDVAKGKSERLRY 167

Query: 122 DEEINLLLSDWWHASIHEQEVGLKSNPMRWVGEPQTILINGTGQFNCTSLDKY--NTSSE 181
           D E NLLLSDWWH +I  QE+GL S PMRW+GE Q+ILING GQFNC+   ++  NTS  
Sbjct: 168 DGEFNLLLSDWWHEAIPSQELGLSSKPMRWIGEAQSILINGRGQFNCSLAAQFSNNTSLP 227

Query: 182 HCEWK--DQCAPFVLKVEPNKTYRIRIASTTSLAALNFAISNHTMLVVEADGNYVQQFVT 241
            C +K  DQCAP +L VEPNKTYRIR++STT+LA+LN A+  H ++VVEADGNY+  F T
Sbjct: 228 MCTFKEGDQCAPQILHVEPNKTYRIRLSSTTALASLNLAVQGHKLVVVEADGNYITPFTT 287

Query: 242 SDIDIYSGESYSVLVTTNQSTTQNYWASIGVRGREPKTSPALLLFNYLPNSVAKLPISPP 301
            DIDIYSGESYSVL+TT+Q  +QNY+ S+GVRGR+P T+ AL + NY+    +KLP SPP
Sbjct: 288 DDIDIYSGESYSVLLTTDQDPSQNYYISVGVRGRKPNTTQALTILNYVTAPASKLPSSPP 347

Query: 302 PEIPKWNDYDRSKNFTYRVLASAMNTTKPPQIFTRRISLLNTQNLMNGNFIKWAINNISL 361
           P  P+W+D++RSKNF+ ++  SAM +  PP+ + +R+ LLNTQNL++G + KWAINN+SL
Sbjct: 348 PVTPRWDDFERSKNFSKKIF-SAMGSPSPPKKYRKRLILLNTQNLIDG-YTKWAINNVSL 407

Query: 362 TLPSTPYLGAIKFGLNSTFNQTAPPETFPKNYNIKIPPPNPNTNTGNSVYRFRSGEIVDV 421
             P+TPYLG++K+ L   FN+ +PP ++  +Y+I  PPP PNT TGN +Y F     VDV
Sbjct: 408 VTPATPYLGSVKYNLKLGFNRKSPPRSYRMDYDIMNPPPFPNTTTGNGIYVFPFNVTVDV 467

Query: 422 ILQNANALSNNTSEIHPWHLHGHDFWVLGYGEGKFSDE-DETKLNLKNPPLKNTVVIFPF 481
           I+QNAN L    SEIHPWHLHGHDFWVLGYG+GKF    DE   NLKNPPL+NT +++P+
Sbjct: 468 IIQNANVLKGIVSEIHPWHLHGHDFWVLGYGDGKFKPGIDEKTYNLKNPPLRNTAILYPY 527

Query: 482 GWTAIRFVADNPGVWIFHCHIEPHLDMGMGVIFAEAVEKVREIPPYALACGRT 530
           GWTAIRFV DNPGVW FHCHIEPHL MGMGV+FAE + ++ ++P  AL CG T
Sbjct: 528 GWTAIRFVTDNPGVWFFHCHIEPHLHMGMGVVFAEGLNRIGKVPDEALGCGLT 578

BLAST of Cucsa.142810 vs. TAIR10
Match: AT5G21100.1 (AT5G21100.1 Plant L-ascorbate oxidase)

HSP 1 Score: 677.6 bits (1747), Expect = 7.4e-195
Identity = 324/536 (60.45%), Postives = 395/536 (73.69%), Query Frame = 1

Query: 2   FWSPDCIENIVMGINGTFPGPPIRANVNDTVVVEVINNLSTEGVVIHWHGILQRGSPWAD 61
           +W PDC E IVM ING FPGP I A   DTV++ V+N LSTEGVVIHWHGI Q+G+PWAD
Sbjct: 34  YWWPDCKEGIVMAINGQFPGPTIDAVAGDTVIIHVVNKLSTEGVVIHWHGIRQKGTPWAD 93

Query: 62  GTASISQCPINPGENFTYEFKVDKPGTYFYHGHFGMQRAAGLYGSLIVDLEEGKKEPFQY 121
           G A ++QCPINPGE FTY+F VDK GT+FYHGH+GMQR++GLYG LIV      KE   Y
Sbjct: 94  GAAGVTQCPINPGETFTYKFIVDKAGTHFYHGHYGMQRSSGLYGMLIV---RSPKERLIY 153

Query: 122 DEEINLLLSDWWHASIHEQEVGLKSNPMRWVGEPQTILINGTGQFNCTSLDKYNTSSEH- 181
           D E NLLLSDWWH SIH QE+ L S PMRW+GEPQ++LING GQFNC+    +N   E  
Sbjct: 154 DGEFNLLLSDWWHQSIHAQELALSSRPMRWIGEPQSLLINGRGQFNCSQAAYFNKGGEKD 213

Query: 182 -CEWK--DQCAPFVLKVEPNKTYRIRIASTTSLAALNFAISNHTMLVVEADGNYVQQFVT 241
            C +K  DQCAP  L+VEPN+ YR+RIASTT+LA+LN A+  H ++VVEADGNYV  F  
Sbjct: 214 VCTFKENDQCAPQTLRVEPNRVYRLRIASTTALASLNLAVQGHQLVVVEADGNYVAPFTV 273

Query: 242 SDIDIYSGESYSVLVTTNQSTTQNYWASIGVRGREPKTSPALLLFNYLPNSVAKLPISPP 301
           +DID+YSGE+YSVL+ TN   ++ YW S+GVRGREPKT  AL + NY+ ++    P  PP
Sbjct: 274 NDIDVYSGETYSVLLKTNALPSKKYWISVGVRGREPKTPQALTVINYV-DATESRPSHPP 333

Query: 302 PEIPKWNDYDRSKNFTYRVLASAMNTTKPPQIFTRRISLLNTQNLMNGNFIKWAINNISL 361
           P  P WND DRSK+F+ ++ A A    KPP+    ++ LLNTQNL   ++ KW+INN+SL
Sbjct: 334 PVTPIWNDTDRSKSFSKKIFA-AKGYPKPPEKSHDQLILLNTQNLYE-DYTKWSINNVSL 393

Query: 362 TLPSTPYLGAIKFGLNSTFNQTAP-PETFPKNYNIKIPPPNPNTNTGNSVYRFRSGEIVD 421
           ++P TPYLG+I++GL S ++  +P  +    NY+I  PPPNPNT  G+ +Y F  G +VD
Sbjct: 394 SVPVTPYLGSIRYGLKSAYDLKSPAKKLIMDNYDIMKPPPNPNTTKGSGIYNFAFGIVVD 453

Query: 422 VILQNANALSNNTSEIHPWHLHGHDFWVLGYGEGKFSDE-DETKLNLKNPPLKNTVVIFP 481
           VILQNAN L    SEIHPWH+HGHDFWVLGYGEGKF    DE   NLKNPPL+NTVV++P
Sbjct: 454 VILQNANVLKGVISEIHPWHIHGHDFWVLGYGEGKFKPGIDEKTFNLKNPPLRNTVVLYP 513

Query: 482 FGWTAIRFVADNPGVWIFHCHIEPHLDMGMGVIFAEAVEKV--REIPPYALACGRT 530
           FGWTAIRFV DNPGVW FHCHIEPHL MGMGV+F E V+++   EIP  AL CG T
Sbjct: 514 FGWTAIRFVTDNPGVWFFHCHIEPHLHMGMGVVFVEGVDRIGKMEIPDEALGCGLT 563

BLAST of Cucsa.142810 vs. TAIR10
Match: AT4G39830.1 (AT4G39830.1 Cupredoxin superfamily protein)

HSP 1 Score: 524.2 bits (1349), Expect = 1.1e-148
Identity = 257/536 (47.95%), Postives = 343/536 (63.99%), Query Frame = 1

Query: 2   FWSPDCIENIVMGINGTFPGPPIRANVNDTVVVEVINNLSTEGVVIHWHGILQRGSPWAD 61
           F SPDC E +V+ ING FPGP I+A   DT+VVE+ N+  TE V +HWHGI Q G+PW D
Sbjct: 46  FKSPDCFEKLVITINGKFPGPTIKAQQGDTIVVELKNSFMTENVAVHWHGIRQIGTPWFD 105

Query: 62  GTASISQCPINPGENFTYEFKVDKPGTYFYHGHFGMQRAAGLYGSLIVDLEEGKKEPFQY 121
           G   ++QCPI PGE F Y+F VD+PGTY YH H+GMQR +GL G + V     + EPF Y
Sbjct: 106 GVEGVTQCPILPGEVFIYQFVVDRPGTYMYHSHYGMQRESGLIGMIQVSPPATEPEPFTY 165

Query: 122 DEEINLLLSDWWHASIHEQEVGLKSNPMRWVGEPQTILINGTGQFNCTS--LDKYNTSSE 181
           D + N LL+DW+H S+ E+  GL S P +WVGEPQ+++I G G+FNC++      +  S 
Sbjct: 166 DYDRNFLLTDWYHKSMSEKATGLASIPFKWVGEPQSLMIQGRGRFNCSNNLTTPPSLVSG 225

Query: 182 HCEWKD-QCAPFVLKVEPNKTYRIRIASTTSLAALNFAISNHTMLVVEADGNYVQQFVTS 241
            C   +  C+ F+L V P KTYR+RI S T+L+AL+F I  H + VVEADG+YV+ F   
Sbjct: 226 VCNVSNADCSRFILTVIPGKTYRLRIGSLTALSALSFQIEGHNLTVVEADGHYVEPFTVK 285

Query: 242 DIDIYSGESYSVLVTTNQSTTQNYWASIGVRGREPKTSPALLLFNYLPNSVAKLPISPPP 301
           ++ +YSGE+YSVL+  +Q+  +NYW +  +  R   T PA  + NY PN   + P +   
Sbjct: 286 NLFVYSGETYSVLLKADQNPRRNYWITSSIVSRPATTPPATAVLNYYPNHPRRRPPTSES 345

Query: 302 E--IPKWNDYDRSKNFTYRVLASAMNTTKPPQIFTRRISLLNTQNLMNGNFIKWAINNIS 361
              +P+WND       +  + A        P+   + I LLNTQN +NG + +W++NN+S
Sbjct: 346 SNIVPEWNDTRSRLAQSLAIKARRGFIHALPENSDKVIVLLNTQNEVNG-YRRWSVNNVS 405

Query: 362 LTLPSTPYLGAIKFGLNSTFNQ--TAPPETFPKNYNIKIPPPNPNTNTGNSVYRFRSGEI 421
              P TPYL A+K  L + F+   TAP     +NY+I   P N N  T + +YR R    
Sbjct: 406 YHHPKTPYLIALKQNLTNAFDWRFTAPENYDSRNYDIFAKPLNANATTSDGIYRLRFNST 465

Query: 422 VDVILQNANALSNNTSEIHPWHLHGHDFWVLGYGEGKFSD-EDETKLNLKNPPLKNTVVI 481
           VDVILQNAN ++ N SE HPWHLHGHDFWVLGYGEGKF++ ED  + N  +P  KNTV +
Sbjct: 466 VDVILQNANTMNANNSETHPWHLHGHDFWVLGYGEGKFNESEDPKRYNRVDPIKKNTVAV 525

Query: 482 FPFGWTAIRFVADNPGVWIFHCHIEPHLDMGMGVIFAEAVEKVREIPPYALACGRT 530
            PFGWTA+RF ADNPGVW FHCHIE H  MGMG++F   ++KV  +P   + CG+T
Sbjct: 526 QPFGWTALRFRADNPGVWSFHCHIESHFFMGMGIVFESGIDKVSSLPSSIMGCGQT 580

BLAST of Cucsa.142810 vs. TAIR10
Match: AT5G03260.1 (AT5G03260.1 laccase 11)

HSP 1 Score: 259.6 bits (662), Expect = 4.8e-69
Identity = 173/522 (33.14%), Postives = 266/522 (50.96%), Query Frame = 1

Query: 7   CIENIVMGINGTFPGPPIRANVNDTVVVEVINNLSTEGVVIHWHGILQRGSPWADGTASI 66
           C    ++ +NG FPGP + A   D V++ V N++    + IHWHG+ Q  + WADG A I
Sbjct: 41  CNAKPIVTVNGMFPGPTVYAREGDRVIINVTNHVQYN-MSIHWHGLKQYRNGWADGPAYI 100

Query: 67  SQCPINPGENFTYEFKVD-KPGTYFYHGHFGMQRAAGLYGSLIVDLEEGKKEPF-QYDEE 126
           +QCPI  G+++ Y+F V  + GT ++H H    RA  +YG++++    GK  PF Q  +E
Sbjct: 101 TQCPIQTGQSYLYDFNVTGQRGTLWWHAHILWLRAT-VYGAIVILPAPGKPYPFPQPYQE 160

Query: 127 INLLLSDWWHASIHEQEVGLKSNPMRWVGEPQTILINGTGQFNCTSLDKYNTSSEHCEWK 186
            N++L +WW+     ++V    N    +G P  +  +     N      +  S +H    
Sbjct: 161 SNIILGEWWN-----KDVETAVNQANQLGAPPPM--SDAHTINGKPGPLFPCSEKHT--- 220

Query: 187 DQCAPFVLKVEPNKTYRIRIASTTSLAALNFAISNHTMLVVEADGNYVQQFVTSDIDIYS 246
                FV++ E  KTY +RI +      L F I+ H M VVE D  Y + F T  I +  
Sbjct: 221 -----FVIEAEAGKTYLLRIINAALNDELFFGIAGHNMTVVEIDAVYTKPFTTKAILLGP 280

Query: 247 GESYSVLVTTNQSTTQNYWASIGVRGR----EPKTSPALLLFNYLPNSVAKLPISPPPEI 306
           G++ +VLV T++S  + + A+          + KT  A+L +  +PN+V  LPI P   +
Sbjct: 281 GQTTNVLVKTDRSPNRYFMAASPFMDAPVSVDNKTVTAILQYKGVPNTV--LPILPKLPL 340

Query: 307 PKWNDYDRSKNFTYRVLASAMNTTKPPQI----------FTRRISLLNTQNLMNGNFIKW 366
           P     D S    Y     ++NT   P +          +T  + +      +NG  +  
Sbjct: 341 PN----DTSFALDYNGKLKSLNTPNFPALVPLKVDRRLFYTIGLGINACPTCVNGTNLAA 400

Query: 367 AINNISLTLPSTPYLGAIKFGLNSTFNQTAPPETFPKNYNIKIPPPNPN--TNTGNSVYR 426
           +INNI+  +P T  L A    ++  F +T  P+  PK +N    P   N  T+TG  + R
Sbjct: 401 SINNITFIMPKTALLKAHYSNISGVF-RTDFPDRPPKAFNYTGVPLTANLGTSTGTRLSR 460

Query: 427 FRSGEIVDVILQNANALSNNTSEIHPWHLHGHDFWVLGYGEGKFSDE-DETKLNLKNPPL 486
            +    ++++LQ+ N L   T E HP+HLHG++F+V+G G G F  + D  K NL +PP 
Sbjct: 461 VKFNTTIELVLQDTNLL---TVESHPFHLHGYNFFVVGTGVGNFDPKKDPAKFNLVDPPE 520

Query: 487 KNTVVIFPFGWTAIRFVADNPGVWIFHCHIEPHLDMGMGVIF 510
           +NTV +   GW AIRF ADNPGVW  HCH+E H   G+ + F
Sbjct: 521 RNTVGVPTGGWAAIRFRADNPGVWFMHCHLEVHTMWGLKMAF 535

BLAST of Cucsa.142810 vs. TAIR10
Match: AT5G05390.1 (AT5G05390.1 laccase 12)

HSP 1 Score: 247.7 bits (631), Expect = 1.9e-65
Identity = 166/530 (31.32%), Postives = 259/530 (48.87%), Query Frame = 1

Query: 7   CIENIVMGINGTFPGPPIRANVNDTVVVEVINNLSTEGVVIHWHGILQRGSPWADGTASI 66
           C     + +NG FPGP +  N  DT+ V+V +N +   + IHWHG+ Q  + WADG   +
Sbjct: 42  CKTRNAITVNGMFPGPTLEVNNGDTLEVKV-HNRARYNITIHWHGVRQIRTGWADGPEFV 101

Query: 67  SQCPINPGENFTYEFKVD-KPGTYFYHGHFGMQRAAGLYGSLIVDLEEGKKEPF-QYDEE 126
           +QCPI PG+++TY F +  + GT ++H H    RA  +YG+LI+    G   PF + D +
Sbjct: 102 TQCPIRPGKSYTYRFTIQGQEGTLWWHAHSSWLRAT-VYGALIIHPTPGSSFPFPKPDRQ 161

Query: 127 INLLLSDWWHASIHEQEVGLKSNPMRWVGEPQTILINGTGQFNCTSLDKYNTSSEHCEWK 186
             L+L +WW+A+     V + +   R    P    I+     N    D YN S++     
Sbjct: 162 TALMLGEWWNAN----PVDVINQATRTGAAPN---ISDAYTINGQPGDLYNCSTKET--- 221

Query: 187 DQCAPFVLKVEPNKTYRIRIASTTSLAALNFAISNHTMLVVEADGNYVQQFVTSDIDIYS 246
                 V+ +   +T  +R+ +      L F ++NH + VV AD +Y++ F T  + +  
Sbjct: 222 -----VVVPINSGETSLLRVINAALNQPLFFTVANHKLTVVGADASYLKPFTTKVLMLGP 281

Query: 247 GESYSVLVTTNQSTTQNYWASIGVRGREPK---TSPALLLFNYLPNSVAKLPISPPPEIP 306
           G++  VL+T +Q   + Y A+   +  +      +    +  Y   +    PI P   +P
Sbjct: 282 GQTTDVLLTADQPPKRYYIAARAYQSAQNAPFDNTTTTAILQYKKTTTTSKPIMPV--LP 341

Query: 307 KWNDYDRSKNFTYRVLASAMNTTKPPQI-----FTRRISLLNTQNL--------MNGNFI 366
            +ND +   +F+ R   S  N   P  I     FT  + L N            +NG   
Sbjct: 342 AFNDTNTVTSFS-RKFKSLRNVVVPKTIDDNLFFTIGLGLDNCPKKFPKSRCQGLNGTRF 401

Query: 367 KWAINNISLTLPST-PYLGAIKFGLNSTFNQTAPPETFPKNYNIKIPPPNPNTNT----- 426
             ++NN+S  LPS    L A   G+   F        FP    +K      N +      
Sbjct: 402 TASMNNVSFVLPSNFSLLQAHSNGIPGVFTTD-----FPSKPPVKFDYTGNNISRALFQP 461

Query: 427 --GNSVYRFRSGEIVDVILQNANALSNNTSEIHPWHLHGHDFWVLGYGEGKFSDEDET-K 486
             G  +Y+ + G  V V+LQ+ N +   TSE HP HLHG+DF+++G G G F+ + +T K
Sbjct: 462 VKGTKLYKLKYGSRVQVVLQDTNIV---TSENHPIHLHGYDFYIVGEGFGNFNPKKDTSK 521

Query: 487 LNLKNPPLKNTVVIFPFGWTAIRFVADNPGVWIFHCHIEPHLDMGMGVIF 510
            NL +PPL+NTV +   GW  IRFVADNPGVW+ HCH++ H+  G+ + F
Sbjct: 522 FNLVDPPLRNTVAVPVNGWAVIRFVADNPGVWLMHCHLDVHIKWGLAMAF 543

BLAST of Cucsa.142810 vs. NCBI nr
Match: gi|778663480|ref|XP_011660094.1| (PREDICTED: L-ascorbate oxidase-like [Cucumis sativus])

HSP 1 Score: 1284.2 bits (3322), Expect = 0.0e+00
Identity = 609/614 (99.19%), Postives = 612/614 (99.67%), Query Frame = 1

Query: 1   MFWSPDCIENIVMGINGTFPGPPIRANVNDTVVVEVINNLSTEGVVIHWHGILQRGSPWA 60
           MFWSPDCIENIVMGINGTFPGPPIRANVNDTVVVEVINNLSTEGVVIHWHGILQRGSPWA
Sbjct: 49  MFWSPDCIENIVMGINGTFPGPPIRANVNDTVVVEVINNLSTEGVVIHWHGILQRGSPWA 108

Query: 61  DGTASISQCPINPGENFTYEFKVDKPGTYFYHGHFGMQRAAGLYGSLIVDLEEGKKEPFQ 120
           DGTASISQCPINPGENFTYEFKVDKPGTYFYHGHFGMQRAAGLYGSLIVDLEEGKKEPFQ
Sbjct: 109 DGTASISQCPINPGENFTYEFKVDKPGTYFYHGHFGMQRAAGLYGSLIVDLEEGKKEPFQ 168

Query: 121 YDEEINLLLSDWWHASIHEQEVGLKSNPMRWVGEPQTILINGTGQFNCTSLDKYNTSSEH 180
           YDEEINLLLSDWWHAS+HEQEVGLKSNPMRWVGEPQTILINGTGQFNCTSL+KYNTSSEH
Sbjct: 169 YDEEINLLLSDWWHASVHEQEVGLKSNPMRWVGEPQTILINGTGQFNCTSLEKYNTSSEH 228

Query: 181 CEWKDQCAPFVLKVEPNKTYRIRIASTTSLAALNFAISNHTMLVVEADGNYVQQFVTSDI 240
           CEWKDQCAPFVLKVEPNKTYRIRIASTTSLAALNFAISNHTMLVVEADGNYVQQFVTSDI
Sbjct: 229 CEWKDQCAPFVLKVEPNKTYRIRIASTTSLAALNFAISNHTMLVVEADGNYVQQFVTSDI 288

Query: 241 DIYSGESYSVLVTTNQSTTQNYWASIGVRGREPKTSPALLLFNYLPNSVAKLPISPPPEI 300
           DIYSGESYSVLVTTNQSTTQNYWASIGVRGREPKTSPALLLFNYLPNSVAKLPISPPPEI
Sbjct: 289 DIYSGESYSVLVTTNQSTTQNYWASIGVRGREPKTSPALLLFNYLPNSVAKLPISPPPEI 348

Query: 301 PKWNDYDRSKNFTYRVLASAMNTTKPPQIFTRRISLLNTQNLMNGNFIKWAINNISLTLP 360
           PKWNDYDRSKNFTYRVLASAMNTTKPP IFTRRISLLNTQNLMNGNFIKWAINNISLTLP
Sbjct: 349 PKWNDYDRSKNFTYRVLASAMNTTKPPPIFTRRISLLNTQNLMNGNFIKWAINNISLTLP 408

Query: 361 STPYLGAIKFGLNSTFNQTAPPETFPKNYNIKIPPPNPNTNTGNSVYRFRSGEIVDVILQ 420
           STPYLGAIKFGLNSTFNQTAPPETFPKNYNIKIPPPNPNTNTGNSVYRFRSGEIVDVILQ
Sbjct: 409 STPYLGAIKFGLNSTFNQTAPPETFPKNYNIKIPPPNPNTNTGNSVYRFRSGEIVDVILQ 468

Query: 421 NANALSNNTSEIHPWHLHGHDFWVLGYGEGKFSDEDETKLNLKNPPLKNTVVIFPFGWTA 480
           NANALSNNTSEIHPWHLHGHDFWVLGYGEGKFSDEDETKLNLKNPPLKNTVVIFPFGWTA
Sbjct: 469 NANALSNNTSEIHPWHLHGHDFWVLGYGEGKFSDEDETKLNLKNPPLKNTVVIFPFGWTA 528

Query: 481 IRFVADNPGVWIFHCHIEPHLDMGMGVIFAEAVEKVREIPPYALACGRTATPQVVPIIPR 540
           IRFVADNPGVWIFHCHIEPHLDMGMGVIFAEAVEKVREIPPYALACGRTATPQVVPIIPR
Sbjct: 529 IRFVADNPGVWIFHCHIEPHLDMGMGVIFAEAVEKVREIPPYALACGRTATPQVVPIIPR 588

Query: 541 PNPPGLDNPYYIIWIGLVIFLCVIGLCYVCLYRIRFNHLSWRPYLGISESQSNQIEFSDL 600
           PNPPGLDNPYYIIWIGLVIFLCVIGLCYVCLYRIRFN+LSWRPYLGISESQSNQIEFSDL
Sbjct: 589 PNPPGLDNPYYIIWIGLVIFLCVIGLCYVCLYRIRFNNLSWRPYLGISESQSNQIEFSDL 648

Query: 601 SQSNPNSNPTHEEP 615
           SQSNPN NPTHEEP
Sbjct: 649 SQSNPNPNPTHEEP 662

BLAST of Cucsa.142810 vs. NCBI nr
Match: gi|700211320|gb|KGN66416.1| (hypothetical protein Csa_1G602530 [Cucumis sativus])

HSP 1 Score: 1275.8 bits (3300), Expect = 0.0e+00
Identity = 609/625 (97.44%), Postives = 612/625 (97.92%), Query Frame = 1

Query: 1   MFWSPDCIENIVMGINGTFPGPPIRANVNDTVVVEVINNLSTEGVVIHWHGILQRGSPWA 60
           MFWSPDCIENIVMGINGTFPGPPIRANVNDTVVVEVINNLSTEGVVIHWHGILQRGSPWA
Sbjct: 49  MFWSPDCIENIVMGINGTFPGPPIRANVNDTVVVEVINNLSTEGVVIHWHGILQRGSPWA 108

Query: 61  DGTASISQCPINPGENFTYEFKVDKPGTYFYHGHFGMQRAAGLYGSLIVDLEEGKKEPFQ 120
           DGTASISQCPINPGENFTYEFKVDKPGTYFYHGHFGMQRAAGLYGSLIVDLEEGKKEPFQ
Sbjct: 109 DGTASISQCPINPGENFTYEFKVDKPGTYFYHGHFGMQRAAGLYGSLIVDLEEGKKEPFQ 168

Query: 121 YDEEINLLLSDWWHASIHEQEVGLKSNPMRWVGEPQTILINGTGQFNCTSLDKYNTSSEH 180
           YDEEINLLLSDWWHAS+HEQEVGLKSNPMRWVGEPQTILINGTGQFNCTSL+KYNTSSEH
Sbjct: 169 YDEEINLLLSDWWHASVHEQEVGLKSNPMRWVGEPQTILINGTGQFNCTSLEKYNTSSEH 228

Query: 181 CEWKDQCAPFVLKVEPNKTYRIRIASTTSLAALNFAIS-----------NHTMLVVEADG 240
           CEWKDQCAPFVLKVEPNKTYRIRIASTTSLAALNFAIS           NHTMLVVEADG
Sbjct: 229 CEWKDQCAPFVLKVEPNKTYRIRIASTTSLAALNFAISKKFHHFSFLFQNHTMLVVEADG 288

Query: 241 NYVQQFVTSDIDIYSGESYSVLVTTNQSTTQNYWASIGVRGREPKTSPALLLFNYLPNSV 300
           NYVQQFVTSDIDIYSGESYSVLVTTNQSTTQNYWASIGVRGREPKTSPALLLFNYLPNSV
Sbjct: 289 NYVQQFVTSDIDIYSGESYSVLVTTNQSTTQNYWASIGVRGREPKTSPALLLFNYLPNSV 348

Query: 301 AKLPISPPPEIPKWNDYDRSKNFTYRVLASAMNTTKPPQIFTRRISLLNTQNLMNGNFIK 360
           AKLPISPPPEIPKWNDYDRSKNFTYRVLASAMNTTKPP IFTRRISLLNTQNLMNGNFIK
Sbjct: 349 AKLPISPPPEIPKWNDYDRSKNFTYRVLASAMNTTKPPPIFTRRISLLNTQNLMNGNFIK 408

Query: 361 WAINNISLTLPSTPYLGAIKFGLNSTFNQTAPPETFPKNYNIKIPPPNPNTNTGNSVYRF 420
           WAINNISLTLPSTPYLGAIKFGLNSTFNQTAPPETFPKNYNIKIPPPNPNTNTGNSVYRF
Sbjct: 409 WAINNISLTLPSTPYLGAIKFGLNSTFNQTAPPETFPKNYNIKIPPPNPNTNTGNSVYRF 468

Query: 421 RSGEIVDVILQNANALSNNTSEIHPWHLHGHDFWVLGYGEGKFSDEDETKLNLKNPPLKN 480
           RSGEIVDVILQNANALSNNTSEIHPWHLHGHDFWVLGYGEGKFSDEDETKLNLKNPPLKN
Sbjct: 469 RSGEIVDVILQNANALSNNTSEIHPWHLHGHDFWVLGYGEGKFSDEDETKLNLKNPPLKN 528

Query: 481 TVVIFPFGWTAIRFVADNPGVWIFHCHIEPHLDMGMGVIFAEAVEKVREIPPYALACGRT 540
           TVVIFPFGWTAIRFVADNPGVWIFHCHIEPHLDMGMGVIFAEAVEKVREIPPYALACGRT
Sbjct: 529 TVVIFPFGWTAIRFVADNPGVWIFHCHIEPHLDMGMGVIFAEAVEKVREIPPYALACGRT 588

Query: 541 ATPQVVPIIPRPNPPGLDNPYYIIWIGLVIFLCVIGLCYVCLYRIRFNHLSWRPYLGISE 600
           ATPQVVPIIPRPNPPGLDNPYYIIWIGLVIFLCVIGLCYVCLYRIRFN+LSWRPYLGISE
Sbjct: 589 ATPQVVPIIPRPNPPGLDNPYYIIWIGLVIFLCVIGLCYVCLYRIRFNNLSWRPYLGISE 648

Query: 601 SQSNQIEFSDLSQSNPNSNPTHEEP 615
           SQSNQIEFSDLSQSNPN NPTHEEP
Sbjct: 649 SQSNQIEFSDLSQSNPNPNPTHEEP 673

BLAST of Cucsa.142810 vs. NCBI nr
Match: gi|659100434|ref|XP_008451098.1| (PREDICTED: L-ascorbate oxidase-like [Cucumis melo])

HSP 1 Score: 1157.5 bits (2993), Expect = 0.0e+00
Identity = 559/626 (89.30%), Postives = 582/626 (92.97%), Query Frame = 1

Query: 1   MFWSPDCIENIVMGINGTFPGPPIRANVNDTVVVEVINNLSTEGVVIHWHGILQRGSPWA 60
           MFWSPDC+ENIV+GINGTFPGPPIRANVNDTVVVEVINNLSTEGVVIHWHGILQRG+PWA
Sbjct: 47  MFWSPDCVENIVLGINGTFPGPPIRANVNDTVVVEVINNLSTEGVVIHWHGILQRGTPWA 106

Query: 61  DGTASISQCPINPGENFTYEFKVDKPGTYFYHGHFGMQRAAGLYGSLIVDLEEGKKEPFQ 120
           DGTASISQCPINPGENFTYEFKVDKPGTYFYHGHFGMQRAAGLYGSLIVDLEEGKKEPFQ
Sbjct: 107 DGTASISQCPINPGENFTYEFKVDKPGTYFYHGHFGMQRAAGLYGSLIVDLEEGKKEPFQ 166

Query: 121 YDEEINLLLSDWWHASIHEQEVGLKSNPMRWVGEPQTILINGTGQFNCTSLDKYNTSSEH 180
           YDEEINLLLSDWWH SI EQEVGLKSNPMRW+GE Q ILINGTGQFNCTSLDKYNTSSEH
Sbjct: 167 YDEEINLLLSDWWHTSIQEQEVGLKSNPMRWIGELQVILINGTGQFNCTSLDKYNTSSEH 226

Query: 181 CEWKDQCAPFVLKVEPNKTYRIRIASTTSLAALNFAISNHTMLVVEADGNYVQQFVTSDI 240
           CEWKDQCAPFVLKVEPNKTYRIRIASTTSL+ALNFAI+NHTMLVVEADGNYV  F TSDI
Sbjct: 227 CEWKDQCAPFVLKVEPNKTYRIRIASTTSLSALNFAIANHTMLVVEADGNYVDPFETSDI 286

Query: 241 DIYSGESYSVLVTTNQSTTQNYWASIGVRGREPKTSPALLLFNYLPNSVAKLPISPPPEI 300
           DIYSGESYSVLVTTNQ+TTQNYWASIGVRGR+PKTSPALLLFNYLPNSVAKLPISPPPE 
Sbjct: 287 DIYSGESYSVLVTTNQATTQNYWASIGVRGRKPKTSPALLLFNYLPNSVAKLPISPPPET 346

Query: 301 PKWNDYDRSKNFTYRVLASAMNTTKPPQIFTRRISLLNTQNLMNGNFIKWAINNISLTLP 360
           PKW+DYDRSK+FT RVLASAMNTTKPP IFTRRISLLNTQN +NG F KWAINNISLTLP
Sbjct: 347 PKWDDYDRSKSFTNRVLASAMNTTKPPPIFTRRISLLNTQNRINGKFTKWAINNISLTLP 406

Query: 361 STPYLGAIKFGLNSTFNQTAPPETFPKNYNIKIPPPNPNTNTGNSVYRFRSGEIVDVILQ 420
           STPYLGAIKFGLNSTFNQT PPETFPKNYNIKIPPPNPNTNTGN VYRFRSGEIVDVILQ
Sbjct: 407 STPYLGAIKFGLNSTFNQTPPPETFPKNYNIKIPPPNPNTNTGNGVYRFRSGEIVDVILQ 466

Query: 421 NANALSNNTSEIHPWHLHGHDFWVLGYGEGKFSDEDETKLNLKNPPLKNTVVIFPFGWTA 480
           NANAL+NNTSEIHPWHLHGHDFWVLG+GEGKFS  DETKLNLKNPPLKNTVVIFPFGWTA
Sbjct: 467 NANALTNNTSEIHPWHLHGHDFWVLGFGEGKFSAADETKLNLKNPPLKNTVVIFPFGWTA 526

Query: 481 IRFVADNPGVWIFHCHIEPHLDMGMGVIFAEAVEKVREIPPYALACGRTATPQVVPIIPR 540
           IRFVADNPGVWIFHCHIEPHLDMGMGVIFAEAVE+VREIPPYALACGRTAT  VVPIIP+
Sbjct: 527 IRFVADNPGVWIFHCHIEPHLDMGMGVIFAEAVERVREIPPYALACGRTATHNVVPIIPQ 586

Query: 541 PNP---PGLDNPYYIIW-IGLVIFLCVIGLCYVCLYRI------RFNHLSWRPYLGISES 600
           P P   PG   PY+++W IGLVI +C IGL  VCLYRI       FN+LSWRPYLG+S+S
Sbjct: 587 PKPPRGPGPVIPYFMLWVIGLVIIVCGIGLFGVCLYRIGLRRGYTFNNLSWRPYLGLSDS 646

Query: 601 QSNQIEFSDLSQSN---PNSNPTHEE 614
           QSNQIEFSDLS+SN     SNPT EE
Sbjct: 647 QSNQIEFSDLSRSNLTQEESNPTQEE 672

BLAST of Cucsa.142810 vs. NCBI nr
Match: gi|4049412|emb|CAA71275.1| (L-ascorbate oxidase [Cucumis melo])

HSP 1 Score: 1152.9 bits (2981), Expect = 0.0e+00
Identity = 557/626 (88.98%), Postives = 581/626 (92.81%), Query Frame = 1

Query: 1   MFWSPDCIENIVMGINGTFPGPPIRANVNDTVVVEVINNLSTEGVVIHWHGILQRGSPWA 60
           MFWSPDC+ENIV+GINGTFPGPPIRANVNDTVVVEVINNLSTEGVVIHWHGILQRG+PWA
Sbjct: 47  MFWSPDCVENIVLGINGTFPGPPIRANVNDTVVVEVINNLSTEGVVIHWHGILQRGTPWA 106

Query: 61  DGTASISQCPINPGENFTYEFKVDKPGTYFYHGHFGMQRAAGLYGSLIVDLEEGKKEPFQ 120
           DGTASISQCPINPGENFTYEFKVDKPGTYFYHGHFGMQRAAGLYGSLIVDLEEGKKEPFQ
Sbjct: 107 DGTASISQCPINPGENFTYEFKVDKPGTYFYHGHFGMQRAAGLYGSLIVDLEEGKKEPFQ 166

Query: 121 YDEEINLLLSDWWHASIHEQEVGLKSNPMRWVGEPQTILINGTGQFNCTSLDKYNTSSEH 180
           YDEEINLLLSDWWH SI EQEVGLKSNPMRW+GE Q ILINGTGQFNCTSLDKYNTSSEH
Sbjct: 167 YDEEINLLLSDWWHTSIQEQEVGLKSNPMRWIGELQVILINGTGQFNCTSLDKYNTSSEH 226

Query: 181 CEWKDQCAPFVLKVEPNKTYRIRIASTTSLAALNFAISNHTMLVVEADGNYVQQFVTSDI 240
           CEWKDQCAPFVLKVEPNKTYRIRIASTTSL+ALNFAI+NHTMLVVEADGNYV  F TSDI
Sbjct: 227 CEWKDQCAPFVLKVEPNKTYRIRIASTTSLSALNFAIANHTMLVVEADGNYVDPFETSDI 286

Query: 241 DIYSGESYSVLVTTNQSTTQNYWASIGVRGREPKTSPALLLFNYLPNSVAKLPISPPPEI 300
           DIYSGESYSVLVTTNQ+TTQNYWASIGVRGR+PKTSPALLLFNYLPNSVAKLPISPPPE 
Sbjct: 287 DIYSGESYSVLVTTNQATTQNYWASIGVRGRKPKTSPALLLFNYLPNSVAKLPISPPPET 346

Query: 301 PKWNDYDRSKNFTYRVLASAMNTTKPPQIFTRRISLLNTQNLMNGNFIKWAINNISLTLP 360
           PKW+DYDRSK+FT RVLASAMNTTKPP IFTRRISLLNTQN +NG F KWAINNISLTLP
Sbjct: 347 PKWDDYDRSKSFTNRVLASAMNTTKPPPIFTRRISLLNTQNRINGKFTKWAINNISLTLP 406

Query: 361 STPYLGAIKFGLNSTFNQTAPPETFPKNYNIKIPPPNPNTNTGNSVYRFRSGEIVDVILQ 420
           STPYLGAIKFGLNSTFNQT PPETFPKNYNIKIPPPNPNTNTGN VYRFRSGEIVDVILQ
Sbjct: 407 STPYLGAIKFGLNSTFNQTPPPETFPKNYNIKIPPPNPNTNTGNGVYRFRSGEIVDVILQ 466

Query: 421 NANALSNNTSEIHPWHLHGHDFWVLGYGEGKFSDEDETKLNLKNPPLKNTVVIFPFGWTA 480
           NANAL+NNTSEIHPWHLHGHDFWVLG+G+ KFS  DETKLNLKNPPLKNTVVIFPFGWTA
Sbjct: 467 NANALTNNTSEIHPWHLHGHDFWVLGFGDFKFSAADETKLNLKNPPLKNTVVIFPFGWTA 526

Query: 481 IRFVADNPGVWIFHCHIEPHLDMGMGVIFAEAVEKVREIPPYALACGRTATPQVVPIIPR 540
           IRFVADNPGVWIFHCHIEPHLDMGMGVIFAEAVE+VREIPPYALACGRTAT  VVPIIP+
Sbjct: 527 IRFVADNPGVWIFHCHIEPHLDMGMGVIFAEAVERVREIPPYALACGRTATHNVVPIIPQ 586

Query: 541 PNP---PGLDNPYYIIW-IGLVIFLCVIGLCYVCLYRI------RFNHLSWRPYLGISES 600
           P P   PG   PY+++W IGLVI +C IGL  VCLYRI       FN+LSWRPYLG+S+S
Sbjct: 587 PKPPRGPGPVIPYFMLWVIGLVIIVCGIGLFGVCLYRIGLRRGYTFNNLSWRPYLGLSDS 646

Query: 601 QSNQIEFSDLSQSN---PNSNPTHEE 614
           QSNQIEFSDLS+SN     SNPT EE
Sbjct: 647 QSNQIEFSDLSRSNLTQEESNPTQEE 672

BLAST of Cucsa.142810 vs. NCBI nr
Match: gi|662033846|ref|NP_001284440.1| (L-ascorbate oxidase-like [Cucumis melo])

HSP 1 Score: 867.1 bits (2239), Expect = 1.9e-248
Identity = 411/533 (77.11%), Postives = 458/533 (85.93%), Query Frame = 1

Query: 1   MFWSPDCIENIVMGINGTFPGPPIRANVNDTVVVEVINNLSTEGVVIHWHGILQRGSPWA 60
           MF SPDC EN+VMGIN  FPGP IRANV DTVVVE+IN LSTE VVIHWHGILQ+G+PWA
Sbjct: 49  MFRSPDCKENLVMGINHQFPGPTIRANVGDTVVVELINKLSTEDVVIHWHGILQKGTPWA 108

Query: 61  DGTASISQCPINPGENFTYEFKVDKPGTYFYHGHFGMQRAAGLYGSLIVDLEEGKKEPFQ 120
           DGTASISQC INPGE+FTY+F VDKPGTYFYHGH GMQRAAGLYGSLIVDLEEGKKEPF 
Sbjct: 109 DGTASISQCAINPGESFTYQFVVDKPGTYFYHGHLGMQRAAGLYGSLIVDLEEGKKEPFH 168

Query: 121 YDEEINLLLSDWWHASIHEQEVGLKSNPMRWVGEPQTILINGTGQFNCTSLDKYNTSSEH 180
           YDEEINLLLSDWWH S+ +QEVGL S+PMRW+GEPQ+ILING GQ NC+   KY  + E 
Sbjct: 169 YDEEINLLLSDWWHESVLKQEVGLSSSPMRWIGEPQSILINGRGQSNCSIGAKYMKNMEQ 228

Query: 181 CE--WKDQCAPFVLKVEPNKTYRIRIASTTSLAALNFAISNHTMLVVEADGNYVQQFVTS 240
           C+    ++CAPF+LKV+PNKTYRIRI STTSL+ALNFAI NH +LVVEADGNYVQ F+TS
Sbjct: 229 CKLSGSEECAPFILKVKPNKTYRIRIISTTSLSALNFAIGNHKLLVVEADGNYVQPFLTS 288

Query: 241 DIDIYSGESYSVLVTTNQSTTQNYWASIGVRGREPKTSPALLLFNYLPNSVAKLPISPPP 300
           DIDIYSGESYSVL+ T+Q+ +QNYW S+GVRGREPKT P L L NYLPNS +KLPISPPP
Sbjct: 289 DIDIYSGESYSVLINTDQNPSQNYWVSLGVRGREPKTPPGLTLLNYLPNSFSKLPISPPP 348

Query: 301 EIPKWNDYDRSKNFTYRVLASAMNTTKPPQIFTRRISLLNTQNLMNGNFIKWAINNISLT 360
           E PKWNDYDRSKNFTYR+LA AM + KPP   TRRI LLNTQN MNG + KWAINN+SLT
Sbjct: 349 ETPKWNDYDRSKNFTYRILA-AMGSPKPPVKSTRRIFLLNTQNKMNGGYTKWAINNVSLT 408

Query: 361 LPSTPYLGAIKFGLNSTFNQTAPPETFPKNYNIKIPPPNPNTNTGNSVYRFRSGEIVDVI 420
           LPSTPYLGAIK G+N TF+Q  PPET+P+NY+I  PPPN NT TGN VYRF+ GEIVDV+
Sbjct: 409 LPSTPYLGAIKIGINDTFDQNPPPETYPENYDIHKPPPNGNTITGNGVYRFQLGEIVDVV 468

Query: 421 LQNANALSNNTSEIHPWHLHGHDFWVLGYGEGKFSDE-DETKLNLKNPPLKNTVVIFPFG 480
           LQNAN L++  SEIHPWHLHGHDFWVLGYGEGKFS   DETKLNLK+PPL+NTVVIFPFG
Sbjct: 469 LQNANTLNDKHSEIHPWHLHGHDFWVLGYGEGKFSAAVDETKLNLKDPPLRNTVVIFPFG 528

Query: 481 WTAIRFVADNPGVWIFHCHIEPHLDMGMGVIFAEAVEKVREIPPYALACGRTA 531
           WT +RFVADNPGVW FHCHIEPHL MGMGV+FAE V+KV  IP +A+ACG TA
Sbjct: 529 WTVLRFVADNPGVWAFHCHIEPHLHMGMGVVFAEGVDKVGRIPTHAMACGGTA 580