Cucsa.141660 (gene) Cucumber (Gy14) v1

Overview
NameCucsa.141660
Typegene
OrganismCucumis sativus (Cucumber (Gy14) v1)
DescriptionBeta-1,3-glucanase
Locationscaffold01079 : 76873 .. 78120 (-)
Genome Viewer
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDS
Hold the cursor over a type above to highlight its positions in the sequence below.
CATACGATATTCTTCTCGGCGCCTACTACGGTCTTGTTGGCGACAACTTGCCTCCACCATGGAAGGTGGTGCAACTCTGTGAACAATACAACATTCGTCGGGTCCGACTAGATGTACCAGACGTGGATGTTTTGACTGCATTTCGTGGTGGGGCTATTGATGTCTCTTTTGGTATACCAAATAACATGCTAACAGATCTGGCCAATAATAAAGGTCTTGTAGAAGAGTGGTTTAATACCTACGTTAAAACCTTTGTTGATGATTTCAGAATCAATTACATCATTGTTGGGGACAAGGCCATCCCTAGCCATGCCAATTTCATATTGCCAATCATGATGTCCCTTCAAGATCTCCTCAATGCAAACTATCTTGGACAAGTGAAGCTCACTACATTGGTTGGGTATGGTTTAAACTAACATTATAAATTTAAAAGGGTTAAGTCAATATGGATCTTAAATCACCAACTAACCCAAAAATTATGAAGTGGGGGTTGAGGTATTAATTAAATCAAATTCATGGCCAAACAAGAGAAGTTCATCCCTTAAATGAGAGAACTCTTCAAGATCCAGAATCAAAATTATACCAAAAGCTTTCCTAGTGAAATTAAAGCAAATTTCATATTGTATCATTGAGGCTAATTTTACTGGTATGGTTGACAGGTACAATGCAGCATTGGTCTCTAAAGACCCGCCATCCAGCGGCACATTCGATCCAACCGTACGTGAGAGCATGAGAGGCATCCTCAAATTCTTAGCAGAAGAAGGCTCAGCTTTGATGGTTAGCGTATTCCCCCATCAAAAGTATGAGTTCAATGGCGGTATAAGTTTGAACTACGCGATATTTAACGAGACAAATCCAGTAGTTCAAGACGAAGGGTTGGAATATTACAACTTGTTCGATGCAATGGTGGATGCATTTTATGCAGCTATCGAAAAAGAAATGGTTGGAGAAGTGAATATTGTAGTTGGAGAAACTGGGTGGCCAACTTGCGGAGACATATCTGGAACACAGGCCATTGCCAAGGAGTACAACAACAAATTTAAGAGTCATATTTCCTCTGGCAACGGAACACCAAGAAAGCCAAATGTTTATCTTGAAGGTTTCATACAAAGTATATTCAACGAAGATAAAAAACCTGAAGGAGATTCACAATGCTATGGGATGTTTGATGTCAACATGAAACCTATTTATCCATTGTTTAGTACTGCAACAGCAGAGAATATGTTCATGTCAGGAAAGAAAAATTAG

mRNA sequence

CATACGATATTCTTCTCGGCGCCTACTACGGTCTTGTTGGCGACAACTTGCCTCCACCATGGAAGGTGGTGCAACTCTGTGAACAATACAACATTCGTCGGGTCCGACTAGATGTACCAGACGTGGATGTTTTGACTGCATTTCGTGGTGGGGCTATTGATGTCTCTTTTGGTATACCAAATAACATGCTAACAGATCTGGCCAATAATAAAGGTCTTGTAGAAGAGTGGTTTAATACCTACGTTAAAACCTTTGTTGATGATTTCAGAATCAATTACATCATTGTTGGGGACAAGGCCATCCCTAGCCATGCCAATTTCATATTGCCAATCATGATGTCCCTTCAAGATCTCCTCAATGCAAACTATCTTGGACAAGTGAAGCTCACTACATTGGTTGGGTACAATGCAGCATTGGTCTCTAAAGACCCGCCATCCAGCGGCACATTCGATCCAACCGTACGTGAGAGCATGAGAGGCATCCTCAAATTCTTAGCAGAAGAAGGCTCAGCTTTGATGGTTAGCGTATTCCCCCATCAAAAGTATGAGTTCAATGGCGGTATAAGTTTGAACTACGCGATATTTAACGAGACAAATCCAGTAGTTCAAGACGAAGGGTTGGAATATTACAACTTGTTCGATGCAATGGTGGATGCATTTTATGCAGCTATCGAAAAAGAAATGGTTGGAGAAGTGAATATTGTAGTTGGAGAAACTGGGTGGCCAACTTGCGGAGACATATCTGGAACACAGGCCATTGCCAAGGAGTACAACAACAAATTTAAGAGTCATATTTCCTCTGGCAACGGAACACCAAGAAAGCCAAATGTTTATCTTGAAGGTTTCATACAAAGTATATTCAACGAAGATAAAAAACCTGAAGGAGATTCACAATGCTATGGGATGTTTGATGTCAACATGAAACCTATTTATCCATTGTTTAGTACTGCAACAGCAGAGAATATGTTCATGTCAGGAAAGAAAAATTAG

Coding sequence (CDS)

CATACGATATTCTTCTCGGCGCCTACTACGGTCTTGTTGGCGACAACTTGCCTCCACCATGGAAGGTGGTGCAACTCTGTGAACAATACAACATTCGTCGGGTCCGACTAGATGTACCAGACGTGGATGTTTTGACTGCATTTCGTGGTGGGGCTATTGATGTCTCTTTTGGTATACCAAATAACATGCTAACAGATCTGGCCAATAATAAAGGTCTTGTAGAAGAGTGGTTTAATACCTACGTTAAAACCTTTGTTGATGATTTCAGAATCAATTACATCATTGTTGGGGACAAGGCCATCCCTAGCCATGCCAATTTCATATTGCCAATCATGATGTCCCTTCAAGATCTCCTCAATGCAAACTATCTTGGACAAGTGAAGCTCACTACATTGGTTGGGTACAATGCAGCATTGGTCTCTAAAGACCCGCCATCCAGCGGCACATTCGATCCAACCGTACGTGAGAGCATGAGAGGCATCCTCAAATTCTTAGCAGAAGAAGGCTCAGCTTTGATGGTTAGCGTATTCCCCCATCAAAAGTATGAGTTCAATGGCGGTATAAGTTTGAACTACGCGATATTTAACGAGACAAATCCAGTAGTTCAAGACGAAGGGTTGGAATATTACAACTTGTTCGATGCAATGGTGGATGCATTTTATGCAGCTATCGAAAAAGAAATGGTTGGAGAAGTGAATATTGTAGTTGGAGAAACTGGGTGGCCAACTTGCGGAGACATATCTGGAACACAGGCCATTGCCAAGGAGTACAACAACAAATTTAAGAGTCATATTTCCTCTGGCAACGGAACACCAAGAAAGCCAAATGTTTATCTTGAAGGTTTCATACAAAGTATATTCAACGAAGATAAAAAACCTGAAGGAGATTCACAATGCTATGGGATGTTTGATGTCAACATGAAACCTATTTATCCATTGTTTAGTACTGCAACAGCAGAGAATATGTTCATGTCAGGAAAGAAAAATTAG

Protein sequence

YDILLGAYYGLVGDNLPPPWKVVQLCEQYNIRRVRLDVPDVDVLTAFRGGAIDVSFGIPNNMLTDLANNKGLVEEWFNTYVKTFVDDFRINYIIVGDKAIPSHANFILPIMMSLQDLLNANYLGQVKLTTLVGYNAALVSKDPPSSGTFDPTVRESMRGILKFLAEEGSALMVSVFPHQKYEFNGGISLNYAIFNETNPVVQDEGLEYYNLFDAMVDAFYAAIEKEMVGEVNIVVGETGWPTCGDISGTQAIAKEYNNKFKSHISSGNGTPRKPNVYLEGFIQSIFNEDKKPEGDSQCYGMFDVNMKPIYPLFSTATAENMFMSGKKN*
Homology
[Close]
BLAST of Cucsa.141660 vs. Swiss-Prot
Match: BG4_ARATH (Probable glucan endo-1,3-beta-glucosidase BG4 OS=Arabidopsis thaliana GN=BG4 PE=2 SV=1)

HSP 1 Score: 221.5 bits (563), Expect = 1.4e-56
Identity = 125/314 (39.81%), Postives = 194/314 (61.78%), Query Frame = 1 

Query: 5   LGAYYGLVGDNLPPPWKVVQLCEQYNIRRVRLDVPDVDVLTAFRGGA-IDVSFGIPNNML 64
           +G  YGL+GDNLP P  V+ L +   I R+R+  P+ +VL A RG   I+V+ G+ +  L
Sbjct: 34  IGLNYGLLGDNLPSPSNVINLYKSIGISRIRIFDPNTEVLNALRGHRDIEVTVGVKDQDL 93

Query: 65  TDLANNKGLVEEWFNTYVKTFVDDFRINYIIVGDKAIPSHAN-FILPIMMSLQDLLNANY 124
             LA ++  V+ WF   +++++ D  I +I VG++ IP      +LP+M SL +L+ +  
Sbjct: 94  AALAASEEAVKGWFAANIESYLADVNITFITVGNEVIPGPIGPQVLPVMQSLTNLVKSRN 153

Query: 125 LGQVKLTTLVGYNAALVSKDPPSSGTFDPTVRESMRGILKFLAEEGSALMVSVFPHQKYE 184
           L  + ++T+V  +  L    PPS+G F    RE +  +LK L++  + ++V+++P+  Y 
Sbjct: 154 L-PISISTVVAMSN-LEQSYPPSAGMFTSQAREQLVPVLKLLSQTSTPILVNIYPYFAYA 213

Query: 185 FN-GGISLNYAIFNETNPVVQDEGLEYYNLFDAMVDAFYAAIEKEMVGEVNIVVGETGWP 244
            +   I L+YA FN  + VVQD  L Y N+FDA+ DAF  A+EKE V  + +VV ETGWP
Sbjct: 214 SDPANIRLDYASFNTKSIVVQDGSLGYSNMFDAIFDAFVWAMEKEGVKNLPMVVSETGWP 273

Query: 245 TCGDIS-GTQAIAKEYNNKFKSHISSGNGTPRKPNVYLEGFIQSIFNEDKKPEGDSQCYG 304
           + G+ +  T AIA  YN  F  HI+SG GTP++PN  + GF+ + FNE++KP G  Q +G
Sbjct: 274 SAGNGNFTTPAIASTYNRNFVKHIASGKGTPKRPNKSMNGFLFATFNENQKPAGTEQNFG 333

Query: 305 MFD-VNMKPIYPLF 314
           +++  +MKPIY LF
Sbjct: 334 LYNPSDMKPIYKLF 345

[Close]
BLAST of Cucsa.141660 vs. Swiss-Prot
Match: BG5_ARATH (Probable glucan endo-1,3-beta-glucosidase BG5 OS=Arabidopsis thaliana GN=BG5 PE=2 SV=1)

HSP 1 Score: 219.2 bits (557), Expect = 6.9e-56
Identity = 126/314 (40.13%), Postives = 191/314 (60.83%), Query Frame = 1 

Query: 5   LGAYYGLVGDNLPPPWKVVQLCEQYNIRRVRLDVPDVDVLTAFRGGA-IDVSFGIPNNML 64
           +G  YGL+GDNLP P KV+ L +  +I ++R+  P+ +VL A RG   I V+ G+ +  L
Sbjct: 43  IGLNYGLLGDNLPSPSKVITLYKSIDITKIRIFDPNTEVLNALRGHRDIAVTVGVRDQDL 102

Query: 65  TDLANNKGLVEEWFNTYVKTFVDDFRINYIIVGDKAIPSHAN-FILPIMMSLQDLLNANY 124
             L+ ++  V+ WF T ++ ++ D  I +I VG++ IP      +LP+M SL +L+ +  
Sbjct: 103 AALSASEEAVKGWFATNIEPYLSDINIAFITVGNEVIPGPIGPQVLPVMQSLTNLVKSRN 162

Query: 125 LGQVKLTTLVGYNAALVSKDPPSSGTFDPTVRESMRGILKFLAEEGSALMVSVFPHQKYE 184
           L     T +  +N  L    PPS+G F    RE +  +LK L++  S ++V ++P+  Y 
Sbjct: 163 LPISISTVVAMWN--LEQSYPPSAGMFTSQAREQLVPVLKLLSQTNSPILVKIYPYFSYA 222

Query: 185 FN-GGISLNYAIFNETNPVVQDEGLEYYNLFDAMVDAFYAAIEKEMVGEVNIVVGETGWP 244
            +   I L+YA FN    VVQD  L Y N+FDA+ DAF  A+EKE V ++ +VV ETGWP
Sbjct: 223 SDPSSIRLDYATFNTEAIVVQDGSLGYSNMFDAIFDAFVWAMEKEGVKDLPMVVSETGWP 282

Query: 245 TCGDIS-GTQAIAKEYNNKFKSHISSGNGTPRKPNVYLEGFIQSIFNEDKKPEGDSQCYG 304
           + G+ +  T  IA  YN  F  HI+SG GTP++PN  ++GF+ + FNE++KP G  Q +G
Sbjct: 283 SAGNGNITTPDIAGTYNRNFVKHIASGKGTPKRPNKGIDGFLFATFNENQKPVGTEQNFG 342

Query: 305 MFDVN-MKPIYPLF 314
           +++ N MKPIY LF
Sbjct: 343 LYNPNDMKPIYNLF 354

[Close]
BLAST of Cucsa.141660 vs. Swiss-Prot
Match: BG1_ARATH (Probable glucan endo-1,3-beta-glucosidase BG1 OS=Arabidopsis thaliana GN=BG1 PE=2 SV=1)

HSP 1 Score: 203.0 bits (515), Expect = 5.1e-51
Identity = 118/314 (37.58%), Postives = 181/314 (57.64%), Query Frame = 1 

Query: 5   LGAYYGLVGDNLPPPWKVVQLCEQYNIRRVRLDVPDVDVLTAFRGGAIDVSFGIPNNMLT 64
           +G  YG  G+NLP P + + L +Q NI+RVRL  PD DVL A RG  I+V+ G+PN+ L 
Sbjct: 27  VGVCYGRNGNNLPSPAETIALFKQKNIQRVRLYSPDHDVLAALRGSNIEVTLGLPNSYLQ 86

Query: 65  DLANNKGLVEEWFNTYVKTFVDDFRINYIIVGD--KAIPSHANFILPIMMSL-QDLLNAN 124
            +A+++     W  TYV  + +  R  YI VG+  K   S+A F++P M ++ + +L A 
Sbjct: 87  SVASSQSQANAWVQTYVMNYANGVRFRYISVGNEVKISDSYAQFLVPAMENIDRAVLAAG 146

Query: 125 YLGQVKLTTLVGYNAALVSKDPPSSGTFDPTVRESMRGILKFLAEEGSALMVSVFPHQKY 184
             G++K++T V     L    PPS G+F   V   M  I++FL  + S L+++++ +  Y
Sbjct: 147 LGGRIKVSTSVDM-GVLRESYPPSKGSFRGDVMVVMEPIIRFLVSKNSPLLLNLYTYFSY 206

Query: 185 EFN-GGISLNYAIFNETNPVVQDEGLEYYNLFDAMVDAFYAAIEKEMVGEVNIVVGETGW 244
             N G I L+YA+F   + +V D    Y NLFDAM+DA Y+A+EK     + IVV ETGW
Sbjct: 207 AGNVGQIRLDYALFTAPSGIVSDPPRSYQNLFDAMLDAMYSALEKSGGASLEIVVAETGW 266

Query: 245 PTCGDISGTQAIAKEYNNKFKSHISSGNGTPRKPNVYLEGFIQSIFNEDKKPEGD--SQC 304
           PT G        A+ YNN    H+   NGTP++P   +E ++ +I++E++KP      + 
Sbjct: 267 PTGGGTDTNIENARIYNNNLIKHVK--NGTPKRPGKEIETYLFAIYDENQKPTPPYVEKF 326

Query: 305 YGMFDVNMKPIYPL 313
           +G+F  N +P Y +
Sbjct: 327 WGLFYPNKQPKYDI 337

[Close]
BLAST of Cucsa.141660 vs. Swiss-Prot
Match: E13A_SOYBN (Glucan endo-1,3-beta-glucosidase OS=Glycine max PE=1 SV=1)

HSP 1 Score: 200.7 bits (509), Expect = 2.5e-50
Identity = 117/317 (36.91%), Postives = 184/317 (58.04%), Query Frame = 1 

Query: 2   DILLGAYYGLVGDNLPPPWKVVQLCEQYNIRRVRLDVPDVDVLTAFRGGAIDVSFGIPNN 61
           D   G  YG +G+NLP P +VV L  Q NIRR+R+  P  +VL A RG  I++   IPN+
Sbjct: 31  DAQSGVCYGRLGNNLPTPQEVVALYNQANIRRMRIYGPSPEVLEALRGSNIELLLDIPND 90

Query: 62  MLTDLANNKGLVEEWFNTYVKTFVDDFRINYIIVGDKAIPSH--ANFILPIMMSLQDLL- 121
            L +LA+++    +W    +K + ++ R  Y+ VG++  P H  A F++P + ++Q  + 
Sbjct: 91  NLRNLASSQDNANKWVQDNIKNYANNVRFRYVSVGNEVKPEHSFAQFLVPALENIQRAIS 150

Query: 122 NANYLGQVKLTTLVGYNAALVSKDPPSSGTFDPTVRES-MRGILKFLAEEGSALMVSVFP 181
           NA    QVK++T +    AL    PPS G+F    R + + G+++FL    + LMV+V+ 
Sbjct: 151 NAGLGNQVKVSTAID-TGALAESFPPSKGSFKSDYRGAYLDGVIRFLVNNNAPLMVNVYS 210

Query: 182 HQKYEFN-GGISLNYAIFNETNPVVQDEGLEYYNLFDAMVDAFYAAIEKEMVGEVNIVVG 241
           +  Y  N   ISL+YA+F   + VVQD  L Y NLFDA VDA YAA+EK   G +NIVV 
Sbjct: 211 YFAYTANPKDISLDYALFRSPSVVVQDGSLGYRNLFDASVDAVYAALEKAGGGSLNIVVS 270

Query: 242 ETGWPTCGDISGTQAIAKEYNNKFKSHISSGNGTPRKPNVYLEGFIQSIFNEDKKPEGDS 301
           E+GWP+ G  + +   A+ YN     ++    GTP++P   LE ++ ++F+E++K     
Sbjct: 271 ESGWPSSGGTATSLDNARTYNTNLVRNVK--QGTPKRPGAPLETYVFAMFDENQKQPEFE 330

Query: 302 QCYGMFD-VNMKPIYPL 313
           + +G+F  +  +P Y +
Sbjct: 331 KFWGLFSPITKQPKYSI 344

[Close]
BLAST of Cucsa.141660 vs. Swiss-Prot
Match: GUB_NICPL (Lichenase OS=Nicotiana plumbaginifolia GN=GN1 PE=2 SV=3)

HSP 1 Score: 194.1 bits (492), Expect = 2.4e-48
Identity = 114/315 (36.19%), Postives = 175/315 (55.56%), Query Frame = 1 

Query: 5   LGAYYGLVGDNLPPPWKVVQLCEQYNIRRVRLDVPDVDVLTAFRGGAIDVSFGIPNNMLT 64
           +G  YG++G+NLPP  +VVQL +  NIRR+RL  P+   L A RG  I+V  G+PN+ L 
Sbjct: 33  VGVCYGMLGNNLPPASQVVQLYKSKNIRRMRLYDPNQAALQALRGSNIEVMLGVPNSDLQ 92

Query: 65  DLANNKGLVEEWFNTYVKTFVDDFRINYIIVGDKAIP-----SHANFILPIMMSLQDLL- 124
           ++A N      W    V+ F    +  YI VG++  P     S   ++LP M ++++ + 
Sbjct: 93  NIAANPSNANNWVQRNVRNFWPAVKFRYIAVGNEVSPVTGTSSLTRYLLPAMRNIRNAIS 152

Query: 125 NANYLGQVKLTTLVGYNAALVSKDPPSSGTFDPTVRESMRGILKFLAEEGSALMVSVFPH 184
           +A     +K+++ V     + +  PPS G+F   VR  +  I+ F+    S L+V+++P+
Sbjct: 153 SAGLQNNIKVSSSVDM-TLIGNSFPPSQGSFRNDVRSFIDPIIGFVRRINSPLLVNIYPY 212

Query: 185 QKYEFN-GGISLNYAIFNETNPVVQDEGLEYYNLFDAMVDAFYAAIEKEMVGEVNIVVGE 244
             Y  N   ISL YA+F   N VVQD  L Y NLFDAM DA YAA+ +   G + IVV E
Sbjct: 213 FSYAGNPRDISLPYALFTAPNVVVQDGSLGYRNLFDAMSDAVYAALSRAGGGSIEIVVSE 272

Query: 245 TGWPTCGDISGTQAIAKEYNNKFKSHISSGNGTPRKPNVYLEGFIQSIFNEDKKPEGDSQ 304
           +GWP+ G  + T   A  Y      H+    G+PR+PN  +E ++ ++F+E+ K     +
Sbjct: 273 SGWPSAGAFAATTNNAATYYKNLIQHVK--RGSPRRPNKVIETYLFAMFDENNKNPELEK 332

Query: 305 CYGMFDVNMKPIYPL 313
            +G+F  N +P YPL
Sbjct: 333 HFGLFSPNKQPKYPL 344

[Close]
BLAST of Cucsa.141660 vs. TrEMBL
Match: A0A0A0LZ10_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_1G595900 PE=3 SV=1)

HSP 1 Score: 670.6 bits (1729), Expect = 9.6e-190
Identity = 328/328 (100.00%), Postives = 328/328 (100.00%), Query Frame = 1 

Query: 1   YDILLGAYYGLVGDNLPPPWKVVQLCEQYNIRRVRLDVPDVDVLTAFRGGAIDVSFGIPN 60
           YDILLGAYYGLVGDNLPPPWKVVQLCEQYNIRRVRLDVPDVDVLTAFRGGAIDVSFGIPN
Sbjct: 24  YDILLGAYYGLVGDNLPPPWKVVQLCEQYNIRRVRLDVPDVDVLTAFRGGAIDVSFGIPN 83

Query: 61  NMLTDLANNKGLVEEWFNTYVKTFVDDFRINYIIVGDKAIPSHANFILPIMMSLQDLLNA 120
           NMLTDLANNKGLVEEWFNTYVKTFVDDFRINYIIVGDKAIPSHANFILPIMMSLQDLLNA
Sbjct: 84  NMLTDLANNKGLVEEWFNTYVKTFVDDFRINYIIVGDKAIPSHANFILPIMMSLQDLLNA 143

Query: 121 NYLGQVKLTTLVGYNAALVSKDPPSSGTFDPTVRESMRGILKFLAEEGSALMVSVFPHQK 180
           NYLGQVKLTTLVGYNAALVSKDPPSSGTFDPTVRESMRGILKFLAEEGSALMVSVFPHQK
Sbjct: 144 NYLGQVKLTTLVGYNAALVSKDPPSSGTFDPTVRESMRGILKFLAEEGSALMVSVFPHQK 203

Query: 181 YEFNGGISLNYAIFNETNPVVQDEGLEYYNLFDAMVDAFYAAIEKEMVGEVNIVVGETGW 240
           YEFNGGISLNYAIFNETNPVVQDEGLEYYNLFDAMVDAFYAAIEKEMVGEVNIVVGETGW
Sbjct: 204 YEFNGGISLNYAIFNETNPVVQDEGLEYYNLFDAMVDAFYAAIEKEMVGEVNIVVGETGW 263

Query: 241 PTCGDISGTQAIAKEYNNKFKSHISSGNGTPRKPNVYLEGFIQSIFNEDKKPEGDSQCYG 300
           PTCGDISGTQAIAKEYNNKFKSHISSGNGTPRKPNVYLEGFIQSIFNEDKKPEGDSQCYG
Sbjct: 264 PTCGDISGTQAIAKEYNNKFKSHISSGNGTPRKPNVYLEGFIQSIFNEDKKPEGDSQCYG 323

Query: 301 MFDVNMKPIYPLFSTATAENMFMSGKKN 329
           MFDVNMKPIYPLFSTATAENMFMSGKKN
Sbjct: 324 MFDVNMKPIYPLFSTATAENMFMSGKKN 351

[Close]
BLAST of Cucsa.141660 vs. TrEMBL
Match: A0A0A0LR03_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_2G416760 PE=3 SV=1)

HSP 1 Score: 363.6 bits (932), Expect = 2.5e-97
Identity = 180/317 (56.78%), Postives = 233/317 (73.50%), Query Frame = 1 

Query: 2   DILLGAYYGLVGDNLPPPWKVVQLCEQYNIRRVRLDVPDVDVLTAFRGGAIDVSFGIPNN 61
           +I +GAYYG  G+NLPPPWKVVQLCE+YNIRR+R + P++D+L  FRG  I+VSF +P  
Sbjct: 26  NIFVGAYYGSEGNNLPPPWKVVQLCEKYNIRRIRFNEPNLDILEQFRGTNIEVSFSVPGE 85

Query: 62  MLTDLANNKGLVEEWFNTYVKTFVDDFRINYIIVGDKAIPSHANFILPIMMSLQDLLNAN 121
           ++ + A N+  VEEWF  YV  F+ +F INYI+VGDK IP   + ILP+M SLQDLLN+ 
Sbjct: 86  LIINQATNQTAVEEWFVKYVVPFIGEFTINYIVVGDKVIPGLDDNILPVMRSLQDLLNSR 145

Query: 122 YLGQVKLTTLVGYNAALVSKDPPSSGTFDPTVRESMRGILKFLAEEGSALMVSVFPHQKY 181
           YLGQVK+TT+VG   AL  + PPSSG FDP V E+M+GILKFL  +GS LM+S++P+  Y
Sbjct: 146 YLGQVKITTMVGL-TALGVQSPPSSGAFDPNVLENMKGILKFLWGQGSPLMLSLYPYDAY 205

Query: 182 EFNG---GISLNYAIF---NETNPVVQDEGLEYY-NLFDAMVDAFYAAIEKEMVGEVNIV 241
            + G    ISL YA F    E NP ++ +G  YY N+FD MVDAF+AAI+K   G+V I 
Sbjct: 206 AYTGYTNNISLGYATFTSQTEQNPPIRIDGNLYYKNVFDDMVDAFHAAIDKACAGDVAIA 265

Query: 242 VGETGWPTCGDISGTQAIAKEYNNKFKSHISSGNGTPRKPNVYLEGFIQSIFNEDKKPEG 301
           +GETGWPT G+     ++A  YN  FK+HI+SG GTP KPN+Y+EGFI+S+FNE++KPEG
Sbjct: 266 IGETGWPTEGNYGAGPSLALTYNLNFKNHITSGKGTPMKPNIYIEGFIRSLFNENEKPEG 325

Query: 302 DSQCYGMFDVNMKPIYP 312
           +SQ YGMF V+  PIYP
Sbjct: 326 ESQFYGMFHVDSTPIYP 341

[Close]
BLAST of Cucsa.141660 vs. TrEMBL
Match: A0A0A0LWM4_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_1G595910 PE=3 SV=1)

HSP 1 Score: 298.5 bits (763), Expect = 9.9e-78
Identity = 151/275 (54.91%), Postives = 195/275 (70.91%), Query Frame = 1 

Query: 44  LTAFRGGAIDVSFGIPNNMLTDLANNKGLVEEWFNTYVKTFVDDFRINYIIVGDKAIPSH 103
           L   RG  I+VSF +P  ++++ A N   VEEWF  YV  F+ +F INYI+VGDK IP  
Sbjct: 3   LRVTRGTNIEVSFSVPGELISNQATNHTAVEEWFVKYVVPFIGEFTINYIVVGDKVIPGL 62

Query: 104 ANFILPIMMSLQDLLNANYLGQVKLTTLVGYNAALVSKDPPSSGTFDPTVRESMRGILKF 163
            + ILP+M SLQDLLN+ YLGQVK+TT+VG  A  V + PPSSG FDP V E+M+GILKF
Sbjct: 63  DDNILPVMRSLQDLLNSRYLGQVKITTMVGLTALGV-QSPPSSGAFDPNVLENMKGILKF 122

Query: 164 LAEEGSALMVSVFPHQKYEFNG---GISLNYAIFN---ETNPVVQDEG-LEYYNLFDAMV 223
           L  +GS LM+S++P+  Y + G    ISL YA F    E NP ++ +G L Y N+FD MV
Sbjct: 123 LWGQGSPLMLSLYPYDAYAYTGYTNNISLGYATFTSQTEQNPPIRTDGDLSYNNIFDEMV 182

Query: 224 DAFYAAIEKEMVGEVNIVVGETGWPTCGDISGTQAIAKEYNNKFKSHISSGNGTPRKPNV 283
           DAFYAAI+K  VG+V I +GETGWPT G+     ++A  YN  FK+HI+SG GTP  PN+
Sbjct: 183 DAFYAAIDKANVGDVAIAIGETGWPTNGNYGAGPSLALTYNLNFKNHITSGKGTPMMPNI 242

Query: 284 YLEGFIQSIFNEDKKPEGDSQCYGMFDVNMKPIYP 312
           Y+EGFI+S+FNE++KPEG+SQ YGMF ++  PIYP
Sbjct: 243 YIEGFIRSLFNENEKPEGESQFYGMFHIDFTPIYP 276

[Close]
BLAST of Cucsa.141660 vs. TrEMBL
Match: M5VWE6_PRUPE (Uncharacterized protein OS=Prunus persica GN=PRUPE_ppa017802mg PE=3 SV=1)

HSP 1 Score: 257.7 bits (657), Expect = 1.9e-65
Identity = 138/312 (44.23%), Postives = 199/312 (63.78%), Query Frame = 1 

Query: 5   LGAYYGLVGDNLPPPWKVVQLCEQYNIRRVRLDVPDVDVLTAFRGGAIDVSFGIPNNMLT 64
           +G  YG++G++LP P +VV L + Y I ++RL  P+ + L A RG  IDVS GI N  L 
Sbjct: 30  IGVCYGMLGNDLPSPTQVVNLYKTYGIGKMRLFDPNPEALQALRGSQIDVSIGIRNEDLP 89

Query: 65  DLANNKGLVEEWFNTYVKTFVDDFRINYIIVGDKAIP-SHANFILPIMMSLQDLLNANYL 124
            LA ++  V  WF T+V+ +++D   N+I VG++ IP S  N++LP+M  LQ +L+   L
Sbjct: 90  SLAVSQDAVNSWFATHVEPYLNDIVFNHITVGNEIIPGSSGNYVLPVMQYLQKILDDRSL 149

Query: 125 GQVKLTTLVGYNAALVSKDPPSSGTFDPTVRESMRGILKFLAEEGSALMVSVFPHQKYEF 184
             +K+TT+V   AAL    PPSSG F      +M GIL  LA+ GS LM++V+P+  Y  
Sbjct: 150 AGIKVTTVVS-GAALGVSYPPSSGAFTLEASSTMSGILPLLAQRGSPLMINVYPYFAYAS 209

Query: 185 NG-GISLNYAIFNETNPVVQDEGLEYYNLFDAMVDAFYAAIEKEMVGEVNIVVGETGWPT 244
           +   + L+YA F  T+PVVQD  L YYNLFDA VDAF AA+EK     V++VV E+GWP+
Sbjct: 210 DPVNVHLDYAQFTATSPVVQDGTLSYYNLFDATVDAFIAAMEKVGGANVDVVVSESGWPS 269

Query: 245 CGDIS-GTQAIAKEYNNKFKSHISSGNGTPRKPNVYLEGFIQSIFNEDKKPEGDSQCYGM 304
            G+    T  +A  YN      ++S  GTP++P  Y+E +I ++FNE++KP+G  Q +G+
Sbjct: 270 DGNGDFTTPELAGTYNRNLLKQVTSRAGTPKRPGAYIEAYIFAMFNENQKPDGVEQHFGL 329

Query: 305 FDVNMKPIYPLF 314
           F  NMKP+YP+F
Sbjct: 330 FHPNMKPVYPVF 340

[Close]
BLAST of Cucsa.141660 vs. TrEMBL
Match: W9QJY8_9ROSA (Putative glucan endo-1,3-beta-glucosidase GVI OS=Morus notabilis GN=L484_027370 PE=3 SV=1)

HSP 1 Score: 250.8 bits (639), Expect = 2.4e-63
Identity = 134/321 (41.74%), Postives = 200/321 (62.31%), Query Frame = 1 

Query: 5   LGAYYGLVGDNLPPPWKVVQLCEQYNIRRVRLDVPDVDVLTAFRGGAIDVSFGIPNNMLT 64
           +G  YG++G+NLPP  +V+ L +  NI ++RL  PD   L A RG  IDV+ GI N  L 
Sbjct: 31  IGVCYGMLGNNLPPATEVINLYKHNNIGKIRLFDPDPSALQALRGSEIDVTVGIRNEDLA 90

Query: 65  DLANNKGLVEEWFNTYVKTFVDDFRINYIIVGDKAIPSH-ANFILPIMMSLQDLLNANYL 124
            LA+N+  V  WF  +V+ +++D   + I VG++ IP H   ++LP+M SLQ +L+   L
Sbjct: 91  GLASNQDAVASWFAAHVEPYLNDIVFSNIAVGNEVIPGHLGQYVLPVMESLQAILDHKNL 150

Query: 125 GQVKLTTLVGYNAALVSKDPPSSGTFDPTVRESMRGILKFLAEEGSALMVSVFPHQKYEF 184
             +KLTT++  NA  +S  PPS+  F       +RGI++F++  G+ LM++V+P+  Y  
Sbjct: 151 AGIKLTTVLPANALALSY-PPSAAQFSDEAANDLRGIIRFMSTHGAPLMINVYPYFAYAS 210

Query: 185 NG-GISLNYAIFNETNPVVQDEGLEYYNLFDAMVDAFYAAIEKEMVGEVNIVVGETGWPT 244
           +   + L+YA+F  T PVVQD  L Y+N+FDA+VDAF+ A E+E V +VNIVV E+GWP+
Sbjct: 211 DPVNVRLDYALFTATAPVVQDGNLSYHNMFDAIVDAFFWATEREGVSDVNIVVAESGWPS 270

Query: 245 CGDIS-GTQAIAKEYNNKFKSHISSGNGTPRKPNVYLEGFIQSIFNEDKKPEGDSQCYGM 304
            G+    T  +A  Y   F +HI S  GTP++P  Y+EGFI ++FNE+ KP G  Q +G+
Sbjct: 271 AGNGGFTTPELASTYAKNFVNHILSNAGTPKRPGPYVEGFIFAMFNENLKPAGVEQNFGL 330

Query: 305 F-----DVNMKPIYPLFSTAT 318
           F        M  +YP F+ A+
Sbjct: 331 FLSTQGSPIMINVYPYFANAS 350

[Close]
BLAST of Cucsa.141660 vs. TAIR10
Match: AT5G20330.1 (AT5G20330.1 beta-1,3-glucanase 4)

HSP 1 Score: 221.5 bits (563), Expect = 7.8e-58
Identity = 125/314 (39.81%), Postives = 194/314 (61.78%), Query Frame = 1 

Query: 5   LGAYYGLVGDNLPPPWKVVQLCEQYNIRRVRLDVPDVDVLTAFRGGA-IDVSFGIPNNML 64
           +G  YGL+GDNLP P  V+ L +   I R+R+  P+ +VL A RG   I+V+ G+ +  L
Sbjct: 34  IGLNYGLLGDNLPSPSNVINLYKSIGISRIRIFDPNTEVLNALRGHRDIEVTVGVKDQDL 93

Query: 65  TDLANNKGLVEEWFNTYVKTFVDDFRINYIIVGDKAIPSHAN-FILPIMMSLQDLLNANY 124
             LA ++  V+ WF   +++++ D  I +I VG++ IP      +LP+M SL +L+ +  
Sbjct: 94  AALAASEEAVKGWFAANIESYLADVNITFITVGNEVIPGPIGPQVLPVMQSLTNLVKSRN 153

Query: 125 LGQVKLTTLVGYNAALVSKDPPSSGTFDPTVRESMRGILKFLAEEGSALMVSVFPHQKYE 184
           L  + ++T+V  +  L    PPS+G F    RE +  +LK L++  + ++V+++P+  Y 
Sbjct: 154 L-PISISTVVAMSN-LEQSYPPSAGMFTSQAREQLVPVLKLLSQTSTPILVNIYPYFAYA 213

Query: 185 FN-GGISLNYAIFNETNPVVQDEGLEYYNLFDAMVDAFYAAIEKEMVGEVNIVVGETGWP 244
            +   I L+YA FN  + VVQD  L Y N+FDA+ DAF  A+EKE V  + +VV ETGWP
Sbjct: 214 SDPANIRLDYASFNTKSIVVQDGSLGYSNMFDAIFDAFVWAMEKEGVKNLPMVVSETGWP 273

Query: 245 TCGDIS-GTQAIAKEYNNKFKSHISSGNGTPRKPNVYLEGFIQSIFNEDKKPEGDSQCYG 304
           + G+ +  T AIA  YN  F  HI+SG GTP++PN  + GF+ + FNE++KP G  Q +G
Sbjct: 274 SAGNGNFTTPAIASTYNRNFVKHIASGKGTPKRPNKSMNGFLFATFNENQKPAGTEQNFG 333

Query: 305 MFD-VNMKPIYPLF 314
           +++  +MKPIY LF
Sbjct: 334 LYNPSDMKPIYKLF 345

[Close]
BLAST of Cucsa.141660 vs. TAIR10
Match: AT5G20390.1 (AT5G20390.1 Glycosyl hydrolase superfamily protein)

HSP 1 Score: 221.5 bits (563), Expect = 7.8e-58
Identity = 123/314 (39.17%), Postives = 190/314 (60.51%), Query Frame = 1 

Query: 5   LGAYYGLVGDNLPPPWKVVQLCEQYNIRRVRLDVPDVDVLTAFRGGA-IDVSFGIPNNML 64
           +G  YGL+GDNLPPP +V+ L +  ++  +R+     DVL AFRG   I V   + N  L
Sbjct: 33  IGLNYGLLGDNLPPPSEVINLYKSLSVTNIRIFDTTTDVLNAFRGNRNIGVMVDVKNQDL 92

Query: 65  TDLANNKGLVEEWFNTYVKTFVDDFRINYIIVGDKAIPSH-ANFILPIMMSLQDLLNANY 124
             L+ ++  V  WF T ++ ++ D  I +I VG++ IP    +++LP+M SL +++ +  
Sbjct: 93  EALSVSEEAVNTWFVTNIEPYLADVNITFIAVGNEVIPGEIGSYVLPVMKSLTNIVKSRS 152

Query: 125 LGQVKLTTLVGYNAALVSKDPPSSGTFDPTVRESMRGILKFLAEEGSALMVSVFPHQKYE 184
           L  +  TT+   N  L    PPS+G F P  RE +  +LKFL++  + ++V+++P+  Y 
Sbjct: 153 LPILISTTVAMTN--LGQSYPPSAGDFMPQAREQLTPVLKFLSQTNTPILVNIYPYFAYA 212

Query: 185 FNG-GISLNYAIFNETNPVVQDEGLEYYNLFDAMVDAFYAAIEKEMVGEVNIVVGETGWP 244
            +   I L+YAIFN    VVQD  L Y N+FD + DAF  A+EKE V ++ +VV ETGWP
Sbjct: 213 ADPINIQLDYAIFNTNKVVVQDGPLGYTNMFDVIFDAFVWAMEKEGVKDLPMVVTETGWP 272

Query: 245 TCGDIS-GTQAIAKEYNNKFKSHISSGNGTPRKPNVYLEGFIQSIFNEDKKPEGDSQCYG 304
           + G+ +  T  IA  YN  F  H+ SG GTP++P   + GF+ + FNE++KP G  Q +G
Sbjct: 273 SAGNGNLTTPDIASIYNTNFVKHVESGKGTPKRPKSGISGFLFATFNENQKPAGTEQNFG 332

Query: 305 MFD-VNMKPIYPLF 314
           +++  +MKPIY +F
Sbjct: 333 LYNPTDMKPIYKMF 344

[Close]
BLAST of Cucsa.141660 vs. TAIR10
Match: AT5G20340.1 (AT5G20340.1 beta-1,3-glucanase 5)

HSP 1 Score: 219.2 bits (557), Expect = 3.9e-57
Identity = 126/314 (40.13%), Postives = 191/314 (60.83%), Query Frame = 1 

Query: 5   LGAYYGLVGDNLPPPWKVVQLCEQYNIRRVRLDVPDVDVLTAFRGGA-IDVSFGIPNNML 64
           +G  YGL+GDNLP P KV+ L +  +I ++R+  P+ +VL A RG   I V+ G+ +  L
Sbjct: 43  IGLNYGLLGDNLPSPSKVITLYKSIDITKIRIFDPNTEVLNALRGHRDIAVTVGVRDQDL 102

Query: 65  TDLANNKGLVEEWFNTYVKTFVDDFRINYIIVGDKAIPSHAN-FILPIMMSLQDLLNANY 124
             L+ ++  V+ WF T ++ ++ D  I +I VG++ IP      +LP+M SL +L+ +  
Sbjct: 103 AALSASEEAVKGWFATNIEPYLSDINIAFITVGNEVIPGPIGPQVLPVMQSLTNLVKSRN 162

Query: 125 LGQVKLTTLVGYNAALVSKDPPSSGTFDPTVRESMRGILKFLAEEGSALMVSVFPHQKYE 184
           L     T +  +N  L    PPS+G F    RE +  +LK L++  S ++V ++P+  Y 
Sbjct: 163 LPISISTVVAMWN--LEQSYPPSAGMFTSQAREQLVPVLKLLSQTNSPILVKIYPYFSYA 222

Query: 185 FN-GGISLNYAIFNETNPVVQDEGLEYYNLFDAMVDAFYAAIEKEMVGEVNIVVGETGWP 244
            +   I L+YA FN    VVQD  L Y N+FDA+ DAF  A+EKE V ++ +VV ETGWP
Sbjct: 223 SDPSSIRLDYATFNTEAIVVQDGSLGYSNMFDAIFDAFVWAMEKEGVKDLPMVVSETGWP 282

Query: 245 TCGDIS-GTQAIAKEYNNKFKSHISSGNGTPRKPNVYLEGFIQSIFNEDKKPEGDSQCYG 304
           + G+ +  T  IA  YN  F  HI+SG GTP++PN  ++GF+ + FNE++KP G  Q +G
Sbjct: 283 SAGNGNITTPDIAGTYNRNFVKHIASGKGTPKRPNKGIDGFLFATFNENQKPVGTEQNFG 342

Query: 305 MFDVN-MKPIYPLF 314
           +++ N MKPIY LF
Sbjct: 343 LYNPNDMKPIYNLF 354

[Close]
BLAST of Cucsa.141660 vs. TAIR10
Match: AT5G20560.1 (AT5G20560.1 Glycosyl hydrolase superfamily protein)

HSP 1 Score: 212.6 bits (540), Expect = 3.6e-55
Identity = 116/305 (38.03%), Postives = 183/305 (60.00%), Query Frame = 1 

Query: 4   LLGAYYGLVGDNLPPPWKVVQLCEQYNIRRVRLDVPDVDVLTAFRGGA-IDVSFGIPNNM 63
           ++G  YGL+GDNLP P  V++  +  N+ ++R+  P+ DVL A RG   I V+ GI N  
Sbjct: 33  VVGLNYGLLGDNLPSPSNVIKFYKSQNVAKIRIFEPNKDVLNALRGNRDIGVTVGIKNED 92

Query: 64  LTDLANNKGLVEEWFNTYVKTFVDDFRINYIIVGDKAIPS--HANFILPIMMSLQDLLNA 123
           L  LA NK  V+ WF+T +  ++ D  I +I VG++AIP   H   +LP++ SL DL+ +
Sbjct: 93  LEALAANKDAVKSWFSTNIDPYIADVNITFITVGNQAIPGDKHGPHVLPVIQSLTDLVKS 152

Query: 124 NYLGQVKLTTLVGYNAALVSKDPPSSGTFDPTVRESMRGILKFLAEEGSALMVSVFPHQK 183
             L Q+ ++T V    +L    PPS+G   P  R+ +  +L+ L++  + + V+++P+  
Sbjct: 153 RNL-QISISTTVT-TTSLARLKPPSAGVLTPQARQQLVPVLRLLSQTSTPIFVNIYPYYF 212

Query: 184 YEFNG-GISLNYAIFNETNPVVQDEGLEYYNLFDAMVDAFYAAIEKEMVGEVNIVVGETG 243
           +  +   + L YA FN    VV+D  L+Y NLFDA+ DAF  A+EKE V  + +VV ETG
Sbjct: 213 HASDPKNVPLEYANFNNDQIVVKDGALKYSNLFDAIFDAFLWAMEKEGVKGLPLVVSETG 272

Query: 244 WPTCGDIS-GTQAIAKEYNNKFKSHISSGNGTPRKPNVYLEGFIQSIFNEDKKPEGDSQC 303
           WP+ G+    T A+   Y   F  H++SG GTP++PN  ++ +I   +NE++KP G  Q 
Sbjct: 273 WPSAGNGGMTTPALQYTYIGNFVKHVASGKGTPKRPNSRIDAYIFETYNENQKPVGIYQH 332

[Close]
BLAST of Cucsa.141660 vs. TAIR10
Match: AT3G57270.1 (AT3G57270.1 beta-1,3-glucanase 1)

HSP 1 Score: 203.0 bits (515), Expect = 2.9e-52
Identity = 118/314 (37.58%), Postives = 181/314 (57.64%), Query Frame = 1 

Query: 5   LGAYYGLVGDNLPPPWKVVQLCEQYNIRRVRLDVPDVDVLTAFRGGAIDVSFGIPNNMLT 64
           +G  YG  G+NLP P + + L +Q NI+RVRL  PD DVL A RG  I+V+ G+PN+ L 
Sbjct: 27  VGVCYGRNGNNLPSPAETIALFKQKNIQRVRLYSPDHDVLAALRGSNIEVTLGLPNSYLQ 86

Query: 65  DLANNKGLVEEWFNTYVKTFVDDFRINYIIVGD--KAIPSHANFILPIMMSL-QDLLNAN 124
            +A+++     W  TYV  + +  R  YI VG+  K   S+A F++P M ++ + +L A 
Sbjct: 87  SVASSQSQANAWVQTYVMNYANGVRFRYISVGNEVKISDSYAQFLVPAMENIDRAVLAAG 146

Query: 125 YLGQVKLTTLVGYNAALVSKDPPSSGTFDPTVRESMRGILKFLAEEGSALMVSVFPHQKY 184
             G++K++T V     L    PPS G+F   V   M  I++FL  + S L+++++ +  Y
Sbjct: 147 LGGRIKVSTSVDM-GVLRESYPPSKGSFRGDVMVVMEPIIRFLVSKNSPLLLNLYTYFSY 206

Query: 185 EFN-GGISLNYAIFNETNPVVQDEGLEYYNLFDAMVDAFYAAIEKEMVGEVNIVVGETGW 244
             N G I L+YA+F   + +V D    Y NLFDAM+DA Y+A+EK     + IVV ETGW
Sbjct: 207 AGNVGQIRLDYALFTAPSGIVSDPPRSYQNLFDAMLDAMYSALEKSGGASLEIVVAETGW 266

Query: 245 PTCGDISGTQAIAKEYNNKFKSHISSGNGTPRKPNVYLEGFIQSIFNEDKKPEGD--SQC 304
           PT G        A+ YNN    H+   NGTP++P   +E ++ +I++E++KP      + 
Sbjct: 267 PTGGGTDTNIENARIYNNNLIKHVK--NGTPKRPGKEIETYLFAIYDENQKPTPPYVEKF 326

Query: 305 YGMFDVNMKPIYPL 313
           +G+F  N +P Y +
Sbjct: 327 WGLFYPNKQPKYDI 337

[Close]
BLAST of Cucsa.141660 vs. NCBI nr
Match: gi|778665329|ref|XP_004144124.2| (PREDICTED: putative glucan endo-1,3-beta-glucosidase GVI [Cucumis sativus])

HSP 1 Score: 670.6 bits (1729), Expect = 1.4e-189
Identity = 328/328 (100.00%), Postives = 328/328 (100.00%), Query Frame = 1 

Query: 1   YDILLGAYYGLVGDNLPPPWKVVQLCEQYNIRRVRLDVPDVDVLTAFRGGAIDVSFGIPN 60
           YDILLGAYYGLVGDNLPPPWKVVQLCEQYNIRRVRLDVPDVDVLTAFRGGAIDVSFGIPN
Sbjct: 24  YDILLGAYYGLVGDNLPPPWKVVQLCEQYNIRRVRLDVPDVDVLTAFRGGAIDVSFGIPN 83

Query: 61  NMLTDLANNKGLVEEWFNTYVKTFVDDFRINYIIVGDKAIPSHANFILPIMMSLQDLLNA 120
           NMLTDLANNKGLVEEWFNTYVKTFVDDFRINYIIVGDKAIPSHANFILPIMMSLQDLLNA
Sbjct: 84  NMLTDLANNKGLVEEWFNTYVKTFVDDFRINYIIVGDKAIPSHANFILPIMMSLQDLLNA 143

Query: 121 NYLGQVKLTTLVGYNAALVSKDPPSSGTFDPTVRESMRGILKFLAEEGSALMVSVFPHQK 180
           NYLGQVKLTTLVGYNAALVSKDPPSSGTFDPTVRESMRGILKFLAEEGSALMVSVFPHQK
Sbjct: 144 NYLGQVKLTTLVGYNAALVSKDPPSSGTFDPTVRESMRGILKFLAEEGSALMVSVFPHQK 203

Query: 181 YEFNGGISLNYAIFNETNPVVQDEGLEYYNLFDAMVDAFYAAIEKEMVGEVNIVVGETGW 240
           YEFNGGISLNYAIFNETNPVVQDEGLEYYNLFDAMVDAFYAAIEKEMVGEVNIVVGETGW
Sbjct: 204 YEFNGGISLNYAIFNETNPVVQDEGLEYYNLFDAMVDAFYAAIEKEMVGEVNIVVGETGW 263

Query: 241 PTCGDISGTQAIAKEYNNKFKSHISSGNGTPRKPNVYLEGFIQSIFNEDKKPEGDSQCYG 300
           PTCGDISGTQAIAKEYNNKFKSHISSGNGTPRKPNVYLEGFIQSIFNEDKKPEGDSQCYG
Sbjct: 264 PTCGDISGTQAIAKEYNNKFKSHISSGNGTPRKPNVYLEGFIQSIFNEDKKPEGDSQCYG 323

Query: 301 MFDVNMKPIYPLFSTATAENMFMSGKKN 329
           MFDVNMKPIYPLFSTATAENMFMSGKKN
Sbjct: 324 MFDVNMKPIYPLFSTATAENMFMSGKKN 351

[Close]
BLAST of Cucsa.141660 vs. NCBI nr
Match: gi|659100567|ref|XP_008451156.1| (PREDICTED: putative glucan endo-1,3-beta-glucosidase GVI [Cucumis melo])

HSP 1 Score: 520.0 bits (1338), Expect = 3.0e-144
Identity = 248/315 (78.73%), Postives = 281/315 (89.21%), Query Frame = 1 

Query: 1   YDILLGAYYGLVGDNLPPPWKVVQLCEQYNIRRVRLDVPDVDVLTAFRGGAIDVSFGIPN 60
           +D+LLGAYYGL G+NLPPPWKVV LCE+YNIRR+RLD+P VDVLTAFRG  ID+SF +PN
Sbjct: 25  HDVLLGAYYGLSGNNLPPPWKVVHLCEKYNIRRIRLDIPTVDVLTAFRGSNIDLSFSVPN 84

Query: 61  NMLTDLANNKGLVEEWFNTYVKTFVDDFRINYIIVGDKAIPSHANFILPIMMSLQDLLNA 120
           +MLTD+A NK LVEEWFNTY+K FVDDF+INYIIVGDKA+P   NFILP+MMSLQDLLNA
Sbjct: 85  DMLTDMATNKALVEEWFNTYIKPFVDDFKINYIIVGDKAVPGLDNFILPVMMSLQDLLNA 144

Query: 121 NYLGQVKLTTLVGYNAALVSKDPPSSGTFDPTVRESMRGILKFLAEEGSALMVSVFPHQK 180
           NY GQVKLTTLVGYNAALVS+DPPSSG F+PTVRE+MRGILKFLAEEGSALMVSVFPH++
Sbjct: 145 NYFGQVKLTTLVGYNAALVSQDPPSSGVFNPTVRENMRGILKFLAEEGSALMVSVFPHRQ 204

Query: 181 YEFNGGISLNYAIFNETNPVVQDEGLEYYNLFDAMVDAFYAAIEKEMVGEVNIVVGETGW 240
           Y+ NGG+SLNYA   ET PVV+D  L+Y NLFDAM+DAFYAAIEKE +GEV +VVGETGW
Sbjct: 205 YKSNGGMSLNYAALKETKPVVEDGELKYSNLFDAMMDAFYAAIEKETIGEVTLVVGETGW 264

Query: 241 PTCGDIS--GTQAIAKEYNNKFKSHISSGNGTPRKPNVYLEGFIQSIFNEDKKPEGDSQC 300
           PTCGD     T  IAK+YNNKFKSHISSG GTPRKPNVYLEGFIQS+FNE++KPEG+SQC
Sbjct: 265 PTCGDNHEIETLDIAKQYNNKFKSHISSGKGTPRKPNVYLEGFIQSLFNENQKPEGESQC 324

Query: 301 YGMFDVNMKPIYPLF 314
           +G+FDVNM PIY LF
Sbjct: 325 FGVFDVNMNPIYSLF 339

[Close]
BLAST of Cucsa.141660 vs. NCBI nr
Match: gi|659100571|ref|XP_008451158.1| (PREDICTED: uncharacterized protein LOC103492536 [Cucumis melo])

HSP 1 Score: 379.8 bits (974), Expect = 4.9e-102
Identity = 192/321 (59.81%), Postives = 242/321 (75.39%), Query Frame = 1 

Query: 1   YDILLGAYYGLVGDNLPPPWKVVQLCEQYNIRRVRLDVPDVDVLTAFRGGAIDVSFGIPN 60
           YD+LLGAYYG  G+NLPPP KVVQLC++YNIRR+R   P+ D++ AFRG  I++SF +  
Sbjct: 336 YDVLLGAYYGTEGNNLPPPEKVVQLCQKYNIRRIRFSEPNFDIIDAFRGKDIELSFSVTG 395

Query: 61  NMLTDLANNKGLVEEWFNTYVKTFVDDFRINYIIVGDKAIPSHANFILPIMMSLQDLLNA 120
            ++T++A N   VEEWF  YV  F+ DF INYIIVGDKAIP   + ILP+M SLQ LLN 
Sbjct: 396 ELITNMATNHTAVEEWFVNYVAPFIGDFTINYIIVGDKAIPGLDDNILPVMKSLQVLLNG 455

Query: 121 NYLGQVKLTTLVGYNAALVSKDPPSSGTFDPTVRESMRGILKFLAEEGSALMVSVFPHQK 180
            YLGQVK+TTLVG  AAL  + PPSSGTFDP V E+MRGIL+FL  +GS LM+S++P+Q+
Sbjct: 456 RYLGQVKITTLVGL-AALGVQTPPSSGTFDPNVLENMRGILQFLHGQGSPLMLSLYPYQE 515

Query: 181 YEFNG---GISLNYAIFN---ETNPVVQDEG-LEYYNLFDAMVDAFYAAIEKEMVGEVNI 240
           Y + G    ISL YA FN   E NP ++  G L Y NLFD MVD FYAAI+K  VG+V I
Sbjct: 516 YAYTGNSNNISLGYANFNSQLEKNPPIRTYGDLSYNNLFDEMVDTFYAAIDKANVGDVPI 575

Query: 241 VVGETGWPTCGDISGTQAIAKEYNNKFKSHISSGNGTPRKPNVYLEGFIQSIFNEDKKPE 300
           V+GETGWPT G+  G+ ++A  YN  FK+HISSG GTP KPN+Y+EGFI+S+FNE++KPE
Sbjct: 576 VIGETGWPTNGNYGGSPSLAATYNRNFKNHISSGKGTPMKPNIYIEGFIRSLFNENEKPE 635

Query: 301 GDSQCYGMFDVNMKPIY-PLF 314
           G+S+ YGMF+V+  PIY P+F
Sbjct: 636 GESRFYGMFNVDSTPIYSPVF 655

[Close]
BLAST of Cucsa.141660 vs. NCBI nr
Match: gi|659100571|ref|XP_008451158.1| (PREDICTED: uncharacterized protein LOC103492536 [Cucumis melo])

HSP 1 Score: 359.8 bits (922), Expect = 5.2e-96
Identity = 180/309 (58.25%), Postives = 232/309 (75.08%), Query Frame = 1 

Query: 1   YDILLGAYYGLVGDNLPPPWKVVQLCEQYNIRRVRLDVPDVDVLTAFRGGAIDVSFGIPN 60
           Y++LLGAYYGL  +NLP PWKV+QLCE+YNIRR+R+D P++DV+ +FRG  I+VSFG+ N
Sbjct: 25  YNVLLGAYYGLESNNLPTPWKVMQLCEKYNIRRIRIDEPNLDVIESFRGTNIEVSFGVSN 84

Query: 61  NMLTDLANNKGLVEEWFNTYVKTFVDDFRINYIIVGDKAIPSHANFILPIMMSLQDLLNA 120
            ++T++A N   VEEWF  Y+  F+DD  INYI+VGDKA+P   + ILP+M SLQDLLN 
Sbjct: 85  GLITNMATNHTAVEEWFANYIAPFIDDLTINYIVVGDKAVPGLDDSILPVMKSLQDLLNR 144

Query: 121 NYLGQVKLTTLVGYNAALVSKDPPSSGTFDPTVRESMRGILKFLAEEGSALMVSVFPHQK 180
            +LGQVK+TTLVG  +AL  K PPSSG FDP V E+MRGIL+FL  +GS LM+S+ P++ 
Sbjct: 145 RFLGQVKITTLVGL-SALGVKAPPSSGAFDPNVFENMRGILQFLWGQGSPLMLSLNPYEA 204

Query: 181 YEFNGGISLNYAIFN---ETNPVVQDEG-LEYYNLFDAMVDAFYAAIEKEMVGEVNIVVG 240
           Y +N  ISL YA F    E NP ++ +G L Y NLFD +VDAFYAAI+K  VG V I +G
Sbjct: 205 YAYN-NISLGYATFTPQLEQNPPIRIDGNLSYNNLFDEVVDAFYAAIDKASVGHVAIAIG 264

Query: 241 ETGWPTCGDISG-TQAIAKEYNNKFKSHISSGNGTPRKPNVYLEGFIQSIFNEDKKPEGD 300
           ETGWPT G   G +  +A  YN  FK+HISSG GTP KPN+Y+EGFI+S+FNE++KPEG+
Sbjct: 265 ETGWPTNGGNDGASPKLAATYNINFKNHISSGKGTPMKPNIYIEGFIKSLFNENEKPEGE 324

Query: 301 SQCYGMFDV 305
           S+ Y M  +
Sbjct: 325 SRFYAMSSI 331


HSP 2 Score: 363.6 bits (932), Expect = 3.6e-97
Identity = 180/317 (56.78%), Postives = 233/317 (73.50%), Query Frame = 1 

Query: 2   DILLGAYYGLVGDNLPPPWKVVQLCEQYNIRRVRLDVPDVDVLTAFRGGAIDVSFGIPNN 61
           +I +GAYYG  G+NLPPPWKVVQLCE+YNIRR+R + P++D+L  FRG  I+VSF +P  
Sbjct: 26  NIFVGAYYGSEGNNLPPPWKVVQLCEKYNIRRIRFNEPNLDILEQFRGTNIEVSFSVPGE 85

Query: 62  MLTDLANNKGLVEEWFNTYVKTFVDDFRINYIIVGDKAIPSHANFILPIMMSLQDLLNAN 121
           ++ + A N+  VEEWF  YV  F+ +F INYI+VGDK IP   + ILP+M SLQDLLN+ 
Sbjct: 86  LIINQATNQTAVEEWFVKYVVPFIGEFTINYIVVGDKVIPGLDDNILPVMRSLQDLLNSR 145

Query: 122 YLGQVKLTTLVGYNAALVSKDPPSSGTFDPTVRESMRGILKFLAEEGSALMVSVFPHQKY 181
           YLGQVK+TT+VG   AL  + PPSSG FDP V E+M+GILKFL  +GS LM+S++P+  Y
Sbjct: 146 YLGQVKITTMVGL-TALGVQSPPSSGAFDPNVLENMKGILKFLWGQGSPLMLSLYPYDAY 205

Query: 182 EFNG---GISLNYAIF---NETNPVVQDEGLEYY-NLFDAMVDAFYAAIEKEMVGEVNIV 241
            + G    ISL YA F    E NP ++ +G  YY N+FD MVDAF+AAI+K   G+V I 
Sbjct: 206 AYTGYTNNISLGYATFTSQTEQNPPIRIDGNLYYKNVFDDMVDAFHAAIDKACAGDVAIA 265

Query: 242 VGETGWPTCGDISGTQAIAKEYNNKFKSHISSGNGTPRKPNVYLEGFIQSIFNEDKKPEG 301
           +GETGWPT G+     ++A  YN  FK+HI+SG GTP KPN+Y+EGFI+S+FNE++KPEG
Sbjct: 266 IGETGWPTEGNYGAGPSLALTYNLNFKNHITSGKGTPMKPNIYIEGFIRSLFNENEKPEG 325

Query: 302 DSQCYGMFDVNMKPIYP 312
           +SQ YGMF V+  PIYP
Sbjct: 326 ESQFYGMFHVDSTPIYP 341

[Close]
BLAST of Cucsa.141660 vs. NCBI nr
Match: gi|778665333|ref|XP_004144115.2| (PREDICTED: lichenase-2-like [Cucumis sativus])

HSP 1 Score: 298.5 bits (763), Expect = 1.4e-77
Identity = 151/275 (54.91%), Postives = 195/275 (70.91%), Query Frame = 1 

Query: 44  LTAFRGGAIDVSFGIPNNMLTDLANNKGLVEEWFNTYVKTFVDDFRINYIIVGDKAIPSH 103
           L   RG  I+VSF +P  ++++ A N   VEEWF  YV  F+ +F INYI+VGDK IP  
Sbjct: 3   LRVTRGTNIEVSFSVPGELISNQATNHTAVEEWFVKYVVPFIGEFTINYIVVGDKVIPGL 62

Query: 104 ANFILPIMMSLQDLLNANYLGQVKLTTLVGYNAALVSKDPPSSGTFDPTVRESMRGILKF 163
            + ILP+M SLQDLLN+ YLGQVK+TT+VG  A  V + PPSSG FDP V E+M+GILKF
Sbjct: 63  DDNILPVMRSLQDLLNSRYLGQVKITTMVGLTALGV-QSPPSSGAFDPNVLENMKGILKF 122

Query: 164 LAEEGSALMVSVFPHQKYEFNG---GISLNYAIFN---ETNPVVQDEG-LEYYNLFDAMV 223
           L  +GS LM+S++P+  Y + G    ISL YA F    E NP ++ +G L Y N+FD MV
Sbjct: 123 LWGQGSPLMLSLYPYDAYAYTGYTNNISLGYATFTSQTEQNPPIRTDGDLSYNNIFDEMV 182

Query: 224 DAFYAAIEKEMVGEVNIVVGETGWPTCGDISGTQAIAKEYNNKFKSHISSGNGTPRKPNV 283
           DAFYAAI+K  VG+V I +GETGWPT G+     ++A  YN  FK+HI+SG GTP  PN+
Sbjct: 183 DAFYAAIDKANVGDVAIAIGETGWPTNGNYGAGPSLALTYNLNFKNHITSGKGTPMMPNI 242

Query: 284 YLEGFIQSIFNEDKKPEGDSQCYGMFDVNMKPIYP 312
           Y+EGFI+S+FNE++KPEG+SQ YGMF ++  PIYP
Sbjct: 243 YIEGFIRSLFNENEKPEGESQFYGMFHIDFTPIYP 276

The following BLAST results are available for this feature:
BLAST of Cucsa.141660 vs. Swiss-Prot
Match NameE-valueIdentityDescription
BG4_ARATH1.4e-5639.81Probable glucan endo-1,3-beta-glucosidase BG4 OS=Arabidopsis thaliana GN=BG4 PE=... [more]
BG5_ARATH6.9e-5640.13Probable glucan endo-1,3-beta-glucosidase BG5 OS=Arabidopsis thaliana GN=BG5 PE=... [more]
BG1_ARATH5.1e-5137.58Probable glucan endo-1,3-beta-glucosidase BG1 OS=Arabidopsis thaliana GN=BG1 PE=... [more]
E13A_SOYBN2.5e-5036.91Glucan endo-1,3-beta-glucosidase OS=Glycine max PE=1 SV=1[more]
GUB_NICPL2.4e-4836.19Lichenase OS=Nicotiana plumbaginifolia GN=GN1 PE=2 SV=3[more]
BLAST of Cucsa.141660 vs. TrEMBL
Match NameE-valueIdentityDescription
A0A0A0LZ10_CUCSA9.6e-190100.00Uncharacterized protein OS=Cucumis sativus GN=Csa_1G595900 PE=3 SV=1[more]
A0A0A0LR03_CUCSA2.5e-9756.78Uncharacterized protein OS=Cucumis sativus GN=Csa_2G416760 PE=3 SV=1[more]
A0A0A0LWM4_CUCSA9.9e-7854.91Uncharacterized protein OS=Cucumis sativus GN=Csa_1G595910 PE=3 SV=1[more]
M5VWE6_PRUPE1.9e-6544.23Uncharacterized protein OS=Prunus persica GN=PRUPE_ppa017802mg PE=3 SV=1[more]
W9QJY8_9ROSA2.4e-6341.74Putative glucan endo-1,3-beta-glucosidase GVI OS=Morus notabilis GN=L484_027370 ... [more]
BLAST of Cucsa.141660 vs. TAIR10
Match NameE-valueIdentityDescription
AT5G20330.17.8e-5839.81 beta-1,3-glucanase 4[more]
AT5G20390.17.8e-5839.17 Glycosyl hydrolase superfamily protein[more]
AT5G20340.13.9e-5740.13 beta-1,3-glucanase 5[more]
AT5G20560.13.6e-5538.03 Glycosyl hydrolase superfamily protein[more]
AT3G57270.12.9e-5237.58 beta-1,3-glucanase 1[more]
BLAST of Cucsa.141660 vs. NCBI nr
Match NameE-valueIdentityDescription
gi|778665329|ref|XP_004144124.2|1.4e-189100.00PREDICTED: putative glucan endo-1,3-beta-glucosidase GVI [Cucumis sativus][more]
gi|659100567|ref|XP_008451156.1|3.0e-14478.73PREDICTED: putative glucan endo-1,3-beta-glucosidase GVI [Cucumis melo][more]
gi|659100571|ref|XP_008451158.1|4.9e-10259.81PREDICTED: uncharacterized protein LOC103492536 [Cucumis melo][more]
gi|659100571|ref|XP_008451158.1|5.2e-9658.25PREDICTED: uncharacterized protein LOC103492536 [Cucumis melo][more]
gi|778665333|ref|XP_004144115.2|1.4e-7754.91PREDICTED: lichenase-2-like [Cucumis sativus][more]
Annotated Terms
The following terms have been associated with this gene:
Vocabulary: INTERPRO
TermDefinition
IPR000490Glyco_hydro_17
IPR013781Glycoside hydrolase, catalytic domain
IPR017853Glycoside_hydrolase_SF
Vocabulary: Molecular Function
TermDefinition
GO:0004553hydrolase activity, hydrolyzing O-glycosyl compounds
Vocabulary: Biological Process
TermDefinition
GO:0005975carbohydrate metabolic process
GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0005975 carbohydrate metabolic process
cellular_component GO:0005575 cellular_component
molecular_function GO:0004553 hydrolase activity, hydrolyzing O-glycosyl compounds
Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Cucsa.141660.1Cucsa.141660.1mRNA


InterPro
Analysis Name: InterPro Annotations of cucumber (Gy14)
Date Performed: 2017-01-17
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR000490Glycoside hydrolase family 17PFAMPF00332Glyco_hydro_17coord: 5..312
score: 8.7
IPR000490Glycoside hydrolase family 17PROSITEPS00587GLYCOSYL_HYDROL_F17coord: 231..244
scor
IPR013781Glycoside hydrolase, catalytic domainGENE3DG3DSA:3.20.20.80coord: 6..312
score: 1.8
IPR017853Glycoside hydrolase superfamilyunknownSSF51445(Trans)glycosidasescoord: 6..312
score: 7.03
NoneNo IPR availablePANTHERPTHR32227FAMILY NOT NAMEDcoord: 1..323
score: 4.8
NoneNo IPR availablePANTHERPTHR32227:SF103BETA-1,3-GLUCANASE 4 (PRECURSOR)-RELATEDcoord: 1..323
score: 4.8

Synteny
The following gene(s) are orthologous to this gene:
GeneOrthologueOrganismBlock
Cucsa.141660Cla011167Watermelon (97103) v1cgywmB251
Cucsa.141660Csa1G595900Cucumber (Chinese Long) v2cgycuB205
Cucsa.141660CSPI01G28610Wild cucumber (PI 183967)cgycpiB208
Cucsa.141660Lsi06G003240Bottle gourd (USVL1VR-Ls)cgylsiB230
Cucsa.141660MELO3C029345.2Melon (DHL92) v3.6.1cgymedB235
Cucsa.141660CsaV3_1G040880Cucumber (Chinese Long) v3cgycucB213
Cucsa.141660Cla97C03G065370Watermelon (97103) v2cgywmbB241
The following gene(s) are paralogous to this gene:

None

The following block(s) are covering this gene:
GeneOrganismBlock
Cucsa.141660Watermelon (97103) v2cgywmbB239
Cucsa.141660Watermelon (97103) v2cgywmbB242
Cucsa.141660Wax gourdcgywgoB279
Cucsa.141660Wax gourdcgywgoB282
Cucsa.141660Cucumber (Gy14) v1cgycgyB091
Cucsa.141660Cucurbita maxima (Rimu)cgycmaB0394
Cucsa.141660Cucurbita maxima (Rimu)cgycmaB0396
Cucsa.141660Cucurbita moschata (Rifu)cgycmoB0393
Cucsa.141660Cucurbita moschata (Rifu)cgycmoB0396
Cucsa.141660Wild cucumber (PI 183967)cgycpiB209
Cucsa.141660Wild cucumber (PI 183967)cgycpiB210
Cucsa.141660Cucumber (Chinese Long) v2cgycuB206
Cucsa.141660Cucumber (Chinese Long) v2cgycuB207
Cucsa.141660Melon (DHL92) v3.5.1cgymeB236
Cucsa.141660Melon (DHL92) v3.5.1cgymeB238
Cucsa.141660Melon (DHL92) v3.5.1cgymeB240
Cucsa.141660Watermelon (Charleston Gray)cgywcgB240
Cucsa.141660Watermelon (Charleston Gray)cgywcgB242
Cucsa.141660Watermelon (97103) v1cgywmB250
Cucsa.141660Watermelon (97103) v1cgywmB252
Cucsa.141660Cucurbita pepo (Zucchini)cgycpeB0383
Cucsa.141660Cucurbita pepo (Zucchini)cgycpeB0385
Cucsa.141660Bottle gourd (USVL1VR-Ls)cgylsiB227
Cucsa.141660Bottle gourd (USVL1VR-Ls)cgylsiB229
Cucsa.141660Melon (DHL92) v3.6.1cgymedB233
Cucsa.141660Melon (DHL92) v3.6.1cgymedB236
Cucsa.141660Silver-seed gourdcarcgyB0112
Cucsa.141660Silver-seed gourdcarcgyB1006
Cucsa.141660Cucumber (Chinese Long) v3cgycucB214
Cucsa.141660Cucumber (Chinese Long) v3cgycucB215