Cucsa.140700 (gene) Cucumber (Gy14) v1

Overview
NameCucsa.140700
Typegene
OrganismCucumis sativus (Cucumber (Gy14) v1)
DescriptionProgesterone 5-beta-reductase
Locationscaffold01071 : 663236 .. 665142 (+)
Genome Viewer
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
CCCCTAATAATTCCCATACCTATCTCCCATTTCTCTTCGGAAAATGAGCTGGTGGTGGGCCGGAGCAATCGGTGCTGCTAAGGTAAAACTCTACAACCCCAATCCCTTTTCTTCTCAACTCTATCCATCTTTTATTCCACTTCTCTCTTCTAATCTTGCAGAAGAAATTTGAAGAAGATGAACCTCCCAGGAGCTTTGAGAGCGTTGCTCTAGTGATTGGCGTTACTGGTATCGTCGGCAACAGTCTTGCCGAGATTCTCCCTCTTTCCGATACCCCCGGTGGTCCATGGAAGGTCTACGGCGTCGCTCGCAGGCCGAGGCCAGCCTGGAATGCTGACCATCCCATCGAATACATTCAATGCGATGTCTCCGATCCCCAAGACGCCGAAACCAAACTCTCCCAGCTCGCTGATGTCACCCATCTCTTCTATGTCACATGGACCAATCGAACCACTGAGATCGAAAACTGTGAGGCCAACGTTAAGATGCTTCGCAACGTTCTGCGATCAGTGATCCCCAACGCACCCAATCTCCGCCATATCTGCCTTCAAACCGGTACGAAGCACTACGTTGGGTCGTTCGAGAGCATTATCAATAAATCGTCGCAGCGGCATGATCCTCCTTTCACAGAGGATTTGCCTCGATTGGAATGCCCAAATTTCTATTACAAACAAGAAGATTTGTTATGGGAAGAGATTGAACAATCTCAGAAAAAGGATCTGACGTGGGCGGTGATTCGACCCAATTTAATTTtCGGGTTTtCCCCTTTCAGCTTGATGAACGTTGTCGGAACTCTATGTGTCTACGCTGCCATATGCAAACACGAGGGACGTCCGTTGAAATTCCCCGGCAATAAATTGGCTTGGGAGGATTTCCAGGTGGCGTCCGACGCAGATCTGATAGCGGAGCAGCACATATGGACGGCGGTGGATCCGTATTCAAAAAACGAGGCGTTTAACTGCAACAATGGCGATGTTTTCAAGTGGAAACACTTCTGGAAGGTGTTGGCAGAGCAGTTTAACATTGAGGAATATGGGTTCGATGAAGAAGGGGAGAGTTTGACACTGGTGGATTTGATGAAGGACAAAAGTGACGTATGGGATGAGATTGTGAAGGAGAACCAATTGCAGCAAACCAAATTGGAGGAGATTGGTACTTGGTGGTTTGTAGATTCAATTTTCAGCATCTCAGGTAACATAGATAGTATGAACAAGAGCAAAGAACATGGGTTTTTGGGCTTCAGGAACTCTAAGAACTCTTTCATTTCTTGGATTGACAAGATTAAGGCTTTcAAAATTGTGCCTTGATTGGCCTTGTAATTCTTGTAGAACTGTTTCATTTGGTTTTATCTGTTTTTATTCCTGGTTTTGCAATAAGCTTGATCACAAGAAAGTGTTGCTTTGTTTACTTTCTTGCTATTTCATAAAGTGATTGTAAAGTGATTGACTTATTTGTGGTTGAAGTCTATAAAGATAATGGAAAATTATTTGTGAGTGTTATCAATAGCATTCTAGATAGTGCTAAAGAGTATTAAAATGAGATGCATGGTTAAACAGTCTCAGTGTCACATCACGAATAGGTTGCTAAATTGGATTTCCATTATCTCTTACAAATGTCTAAGTTATTGGAGATTCTAAGTAGTGGACTTCCCCATTGTCCGTCCTAACTTTCACTCTCTTAACTAACTTTTTCCACTGAGAAGAGTTTTCATTTATGTTGTTACTCAAGTTAATTCTAAAATTAAGATCTCTGTATATTATTTGCCTTAACTACAACAAATAACATTTTCAACAGGGTCCTTTCCTATAGCATTAAACTGAAAATGCTAATGAAAAATTGGAAGAAAAAAAaGGCGCCTTGTCCTTTTTTATAACTTTTAGTTTCTAGACTGAAAGTGAGGTTTTTTT

mRNA sequence

CCCCTAATAATTCCCATACCTATCTCCCATTTCTCTTCGGAAAATGAGCTGGTGGTGGGCCGGAGCAATCGGTGCTGCTAAGAAGAAATTTGAAGAAGATGAACCTCCCAGGAGCTTTGAGAGCGTTGCTCTAGTGATTGGCGTTACTGGTATCGTCGGCAACAGTCTTGCCGAGATTCTCCCTCTTTCCGATACCCCCGGTGGTCCATGGAAGGTCTACGGCGTCGCTCGCAGGCCGAGGCCAGCCTGGAATGCTGACCATCCCATCGAATACATTCAATGCGATGTCTCCGATCCCCAAGACGCCGAAACCAAACTCTCCCAGCTCGCTGATGTCACCCATCTCTTCTATGTCACATGGACCAATCGAACCACTGAGATCGAAAACTGTGAGGCCAACGTTAAGATGCTTCGCAACGTTCTGCGATCAGTGATCCCCAACGCACCCAATCTCCGCCATATCTGCCTTCAAACCGGTACGAAGCACTACGTTGGGTCGTTCGAGAGCATTATCAATAAATCGTCGCAGCGGCATGATCCTCCTTTCACAGAGGATTTGCCTCGATTGGAATGCCCAAATTTCTATTACAAACAAGAAGATTTGTTATGGGAAGAGATTGAACAATCTCAGAAAAAGGATCTGACGTGGGCGGTGATTCGACCCAATTTAATTTTCGGGTTTTCCCCTTTCAGCTTGATGAACGTTGTCGGAACTCTATGTGTCTACGCTGCCATATGCAAACACGAGGGACGTCCGTTGAAATTCCCCGGCAATAAATTGGCTTGGGAGGATTTCCAGGTGGCGTCCGACGCAGATCTGATAGCGGAGCAGCACATATGGACGGCGGTGGATCCGTATTCAAAAAACGAGGCGTTTAACTGCAACAATGGCGATGTTTTCAAGTGGAAACACTTCTGGAAGGTGTTGGCAGAGCAGTTTAACATTGAGGAATATGGGTTCGATGAAGAAGGGGAGAGTTTGACACTGGTGGATTTGATGAAGGACAAAAGTGACGTATGGGATGAGATTGTGAAGGAGAACCAATTGCAGCAAACCAAATTGGAGGAGATTGGTACTTGGTGGTTTGTAGATTCAATTTTCAGCATCTCAGGTAACATAGATAGTATGAACAAGAGCAAAGAACATGGGTTTTTGGGCTTCAGGAACTCTAAGAACTCTTTCATTTCTTGGATTGACAAGATTAAGGCTTTCAAAATTGTGCCTTGATTGGCCTTGTAATTCTTGTAGAACTGTTTCATTTGGTTTTATCTGTTTTTATTCCTGGTTTTGCAATAAGCTTGATCACAAGAAAGTGTTGCTTTGTTTACTTTCTTGCTATTTCATAAAGTGATTGTAAAGTGATTGACTTATTTGTGGTTGAAGTCTATAAAGATAATGGAAAATTATTTGTGAGTGTTATCAATAGCATTCTAGATAGTGCTAAAGAGTATTAAAATGAGATGCATGGTTAAACAGTCTCAGTGTCACATCACGAATAGGTTGCTAAATTGGATTTCCATTATCTCTTACAAATGTCTAAGTTATTGGAGATTCTAAGTAGTGGACTTCCCCATTGTCCGTCCTAACTTTCACTCTCTTAACTAACTTTTTCCACTGAGAAGAGTTTTCATTTATGTTGTTACTCAAGTTAATTCTAAAATTAAGATCTCTGTATATTATTTGCCTTAACTACAACAAATAACATTTTCAACAGGGTCCTTTCCTATAGCATTAAACTGAAAATGCTAATGAAAAATTGGAAGAAAAAAAAGGCGCCTTGTCCTTTTTTATAACTTTTAGTTTCTAGACTGAAAGTGAGGTTTTTTT

Coding sequence (CDS)

ATGAGCTGGTGGTGGGCCGGAGCAATCGGTGCTGCTAAGAAGAAATTTGAAGAAGATGAACCTCCCAGGAGCTTTGAGAGCGTTGCTCTAGTGATTGGCGTTACTGGTATCGTCGGCAACAGTCTTGCCGAGATTCTCCCTCTTTCCGATACCCCCGGTGGTCCATGGAAGGTCTACGGCGTCGCTCGCAGGCCGAGGCCAGCCTGGAATGCTGACCATCCCATCGAATACATTCAATGCGATGTCTCCGATCCCCAAGACGCCGAAACCAAACTCTCCCAGCTCGCTGATGTCACCCATCTCTTCTATGTCACATGGACCAATCGAACCACTGAGATCGAAAACTGTGAGGCCAACGTTAAGATGCTTCGCAACGTTCTGCGATCAGTGATCCCCAACGCACCCAATCTCCGCCATATCTGCCTTCAAACCGGTACGAAGCACTACGTTGGGTCGTTCGAGAGCATTATCAATAAATCGTCGCAGCGGCATGATCCTCCTTTCACAGAGGATTTGCCTCGATTGGAATGCCCAAATTTCTATTACAAACAAGAAGATTTGTTATGGGAAGAGATTGAACAATCTCAGAAAAAGGATCTGACGTGGGCGGTGATTCGACCCAATTTAATTTtCGGGTTTtCCCCTTTCAGCTTGATGAACGTTGTCGGAACTCTATGTGTCTACGCTGCCATATGCAAACACGAGGGACGTCCGTTGAAATTCCCCGGCAATAAATTGGCTTGGGAGGATTTCCAGGTGGCGTCCGACGCAGATCTGATAGCGGAGCAGCACATATGGACGGCGGTGGATCCGTATTCAAAAAACGAGGCGTTTAACTGCAACAATGGCGATGTTTTCAAGTGGAAACACTTCTGGAAGGTGTTGGCAGAGCAGTTTAACATTGAGGAATATGGGTTCGATGAAGAAGGGGAGAGTTTGACACTGGTGGATTTGATGAAGGACAAAAGTGACGTATGGGATGAGATTGTGAAGGAGAACCAATTGCAGCAAACCAAATTGGAGGAGATTGGTACTTGGTGGTTTGTAGATTCAATTTTCAGCATCTCAGGTAACATAGATAGTATGAACAAGAGCAAAGAACATGGGTTTTTGGGCTTCAGGAACTCTAAGAACTCTTTCATTTCTTGGATTGACAAGATTAAGGCTTTcAAAATTGTGCCTTGA

Protein sequence

MSWWWAGAIGAAKKKFEEDEPPRSFESVALVIGVTGIVGNSLAEILPLSDTPGGPWKVYGVARRPRPAWNADHPIEYIQCDVSDPQDAETKLSQLADVTHLFYVTWTNRTTEIENCEANVKMLRNVLRSVIPNAPNLRHICLQTGTKHYVGSFESIINKSSQRHDPPFTEDLPRLECPNFYYKQEDLLWEEIEQSQKKDLTWAVIRPNLIFGFSPFSLMNVVGTLCVYAAICKHEGRPLKFPGNKLAWEDFQVASDADLIAEQHIWTAVDPYSKNEAFNCNNGDVFKWKHFWKVLAEQFNIEEYGFDEEGESLTLVDLMKDKSDVWDEIVKENQLQQTKLEEIGTWWFVDSIFSISGNIDSMNKSKEHGFLGFRNSKNSFISWIDKIKAFKIVP*
Homology
[Close]
BLAST of Cucsa.140700 vs. Swiss-Prot
Match: VEP1_ARATH (3-oxo-Delta(4,5)-steroid 5-beta-reductase OS=Arabidopsis thaliana GN=VEP1 PE=1 SV=1)

HSP 1 Score: 641.7 bits (1654), Expect = 5.2e-183
Identity = 298/394 (75.63%), Postives = 338/394 (85.79%), Query Frame = 1 

Query: 1   MSWWWAGAIGAAKKKFEEDEPPRSFESVALVIGVTGIVGNSLAEILPLSDTPGGPWKVYG 60
           MSWWWAGAIGAAKKK +EDEP +SFESVAL+IGVTGIVGNSLAEILPLSDTPGGPWKVYG
Sbjct: 1   MSWWWAGAIGAAKKKLDEDEPSQSFESVALIIGVTGIVGNSLAEILPLSDTPGGPWKVYG 60

Query: 61  VARRPRPAWNADHPIEYIQCDVSDPQDAETKLSQLADVTHLFYVTWTNRTTEIENCEANV 120
           VARRPRP WNADHPI+YIQCDVSD +D  +KLS L DVTH+FYVTWTNR +E ENCEAN 
Sbjct: 61  VARRPRPTWNADHPIDYIQCDVSDAEDTRSKLSPLTDVTHVFYVTWTNRESESENCEANG 120

Query: 121 KMLRNVLRSVIPNAPNLRHICLQTGTKHYVGSFESIINKSSQRHDPPFTEDLPRLECPNF 180
            MLRNVL+++IP APNLRH+CLQTGTKHY+G F    N    RHDPPFTED+PRL+  NF
Sbjct: 121 SMLRNVLQAIIPYAPNLRHVCLQTGTKHYLGPF---TNVDGPRHDPPFTEDMPRLQIQNF 180

Query: 181 YYKQEDLLWEEIEQSQKKDLTWAVIRPNLIFGFSPFSLMNVVGTLCVYAAICKHEGRPLK 240
           YY QED+L+EEI++ +   +TW++ RPN+IFGFSP+SLMN+VGTLCVYAAICKHEG PL 
Sbjct: 181 YYTQEDILFEEIKKIE--TVTWSIHRPNMIFGFSPYSLMNIVGTLCVYAAICKHEGSPLL 240

Query: 241 FPGNKLAWEDFQVASDADLIAEQHIWTAVDPYSKNEAFNCNNGDVFKWKHFWKVLAEQFN 300
           FPG+K AWE F  ASDADLIAEQ IW AVDPY+KNEAFNCNN D+FKWKH WK+LAEQF 
Sbjct: 241 FPGSKKAWEGFMTASDADLIAEQQIWAAVDPYAKNEAFNCNNADIFKWKHLWKILAEQFG 300

Query: 301 IEEYGFDEEGESLTLVDLMKDKSDVWDEIVKENQLQQTKLEEIGTWWFVDSIFSISGNID 360
           IEEYGF EEG++L LV++MK K  VW+E+VKENQLQ+ KLEE+G WWF D I  + G ID
Sbjct: 301 IEEYGF-EEGKNLGLVEMMKGKERVWEEMVKENQLQEKKLEEVGVWWFADVILGVEGMID 360

Query: 361 SMNKSKEHGFLGFRNSKNSFISWIDKIKAFKIVP 395
           SMNKSKE+GFLGFRNS NSFISWIDK KAFKIVP
Sbjct: 361 SMNKSKEYGFLGFRNSNNSFISWIDKYKAFKIVP 388

[Close]
BLAST of Cucsa.140700 vs. Swiss-Prot
Match: 5BPOR_DIGLA (3-oxo-Delta(4,5)-steroid 5-beta-reductase OS=Digitalis lanata PE=1 SV=1)

HSP 1 Score: 572.8 bits (1475), Expect = 2.9e-162
Identity = 272/394 (69.04%), Postives = 316/394 (80.20%), Query Frame = 1 

Query: 1   MSWWWAGAIGAAKKKFEEDEPPRSFESVALVIGVTGIVGNSLAEILPLSDTPGGPWKVYG 60
           MSWWWAGAIGAAKK+ EED+      SVAL++GVTGI+GNSLAEILPL+DTPGGPWKVYG
Sbjct: 1   MSWWWAGAIGAAKKRLEEDDAQPKHSSVALIVGVTGIIGNSLAEILPLADTPGGPWKVYG 60

Query: 61  VARRPRPAWNADHPIEYIQCDVSDPQDAETKLSQLADVTHLFYVTWTNRTTEIENCEANV 120
           VARR RPAW+ D+PI Y+QCD+SDP D++ KLS L DVTH+FYVTW NR+TE ENCEAN 
Sbjct: 61  VARRTRPAWHEDNPINYVQCDISDPDDSQAKLSPLTDVTHVFYVTWANRSTEQENCEANS 120

Query: 121 KMLRNVLRSVIPNAPNLRHICLQTGTKHYVGSFESIINKSSQRHDPPFTEDLPRLECPNF 180
           KM RNVL +VIPN PNL+HI LQTG KHY+G FES      + HDPP+TEDLPRL+  NF
Sbjct: 121 KMFRNVLDAVIPNCPNLKHISLQTGRKHYMGPFESY--GKIESHDPPYTEDLPRLKYMNF 180

Query: 181 YYKQEDLLWEEIEQSQKKDLTWAVIRPNLIFGFSPFSLMNVVGTLCVYAAICKHEGRPLK 240
           YY  ED++ EE+E  +K+ LTW+V RP  IFGFSP+S+MN+VGTLCVYAAICKHEG+ L+
Sbjct: 181 YYDLEDIMLEEVE--KKEGLTWSVHRPGNIFGFSPYSMMNLVGTLCVYAAICKHEGKVLR 240

Query: 241 FPGNKLAWEDFQVASDADLIAEQHIWTAVDPYSKNEAFNCNNGDVFKWKHFWKVLAEQFN 300
           F G K AW+ +   SDADLIAE HIW AVDPY+KNEAFN +NGDVFKWKHFWKVLAEQF 
Sbjct: 241 FTGCKAAWDGYSDCSDADLIAEHHIWAAVDPYAKNEAFNVSNGDVFKWKHFWKVLAEQFG 300

Query: 301 IEEYGFDEEGESLTLVDLMKDKSDVWDEIVKENQLQQTKLEEIGTWWFVDSIFSISGNID 360
           +   G  EEG  L L DLMK K  VW+EIV+EN L  TKL+++G WWF D I      +D
Sbjct: 301 V-GCGEYEEGVDLKLQDLMKGKEPVWEEIVRENGLTPTKLKDVGIWWFGDVILGNECFLD 360

Query: 361 SMNKSKEHGFLGFRNSKNSFISWIDKIKAFKIVP 395
           SMNKSKEHGFLGFRNSKN+FISWIDK KA+KIVP
Sbjct: 361 SMNKSKEHGFLGFRNSKNAFISWIDKAKAYKIVP 389

[Close]
BLAST of Cucsa.140700 vs. Swiss-Prot
Match: IRIS_CATRO (Iridoid synthase OS=Catharanthus roseus PE=1 SV=1)

HSP 1 Score: 485.0 bits (1247), Expect = 8.1e-136
Identity = 234/397 (58.94%), Postives = 287/397 (72.29%), Query Frame = 1 

Query: 1   MSWWWAGAIGAAKK---KFEEDEPPRSFESVALVIGVTGIVGNSLAEILPLSDTPGGPWK 60
           MSWWW  +IGA K    + +E+   +S++SVALV+GVTGIVG+SLAE+L L DTPGGPWK
Sbjct: 1   MSWWWKRSIGAGKNLPNQNKENGVCKSYKSVALVVGVTGIVGSSLAEVLKLPDTPGGPWK 60

Query: 61  VYGVARRPRPAWNADHPIEYIQCDVSDPQDAETKLSQLADVTHLFYVTWTNRTTEIENCE 120
           VYGVARRP P W A  P+EYIQCDVSD Q+  +KLS L D+TH+FYV+W       E+C+
Sbjct: 61  VYGVARRPCPVWLAKKPVEYIQCDVSDNQETISKLSPLKDITHIFYVSWIGS----EDCQ 120

Query: 121 ANVKMLRNVLRSVIPNAPNLRHICLQTGTKHYVGSFESIINKSSQRHDPPFTEDLPRLEC 180
            N  M +N+L SVIPNA NL+H+CLQTG KHY G FE         HD PFTEDLPRL  
Sbjct: 121 TNATMFKNILNSVIPNASNLQHVCLQTGIKHYFGIFEE--GSKVVPHDSPFTEDLPRLNV 180

Query: 181 PNFYYKQEDLLWEEIEQSQKKDLTWAVIRPNLIFGFSPFSLMNVVGTLCVYAAICKHEGR 240
           PNFY+  ED+L+EE   + K +LTW+V RP L+FGFSP S+MN+V TLCVYA ICKHE +
Sbjct: 181 PNFYHDLEDILYEE---TGKNNLTWSVHRPALVFGFSPCSMMNIVSTLCVYATICKHENK 240

Query: 241 PLKFPGNKLAWEDFQVASDADLIAEQHIWTAVDPYSKNEAFNCNNGDVFKWKHFWKVLAE 300
            L +PG+K +W  +  A DADL+AE  IW AVDP +KN+  NCNNGDVFKWKH WK LAE
Sbjct: 241 ALVYPGSKNSWNCYADAVDADLVAEHEIWAAVDPKAKNQVLNCNNGDVFKWKHIWKKLAE 300

Query: 301 QFNIEEYGFDEEGESLTLVDLMKDKSDVWDEIVKENQLQQTKLEEIGTWWFVDSIFSISG 360
           +F IE  G+ E  E ++L +LMKDK  VWDEIVK+N L  TKL+EI  +WF D  F    
Sbjct: 301 EFGIEMVGYVEGKEQVSLAELMKDKDQVWDEIVKKNNLVPTKLKEIAAFWFADIAFCSEN 360

Query: 361 NIDSMNKSKEHGFLGFRNSKNSFISWIDKIKAFKIVP 395
            I SMNKSKE GFLGFRNS  SF+S IDK++ ++ +P
Sbjct: 361 LISSMNKSKELGFLGFRNSMKSFVSCIDKMRDYRFIP 388

[Close]
BLAST of Cucsa.140700 vs. Swiss-Prot
Match: YJ72_SCHPO (Uncharacterized protein C757.02c OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) GN=SPCC757.02c PE=4 SV=1)

HSP 1 Score: 134.0 bits (336), Expect = 3.5e-30
Identity = 110/410 (26.83%), Postives = 176/410 (42.93%), Query Frame = 1 

Query: 28  VALVIGVTGIVGNSLAEILPLSDTPGGPWKVYGVARRPRPAWNADHPIEYIQCDVSDPQD 87
           VA+V G TG+ G ++ + L   D       +    +   P     H I+ +     +P+D
Sbjct: 7   VAIVTGATGLNGAAIIKRLSEDDNCKTIHCISRSLKDEYPRKIKHHSIDLLN---EEPKD 66

Query: 88  AETKLSQ--LADVTHLFYVTWTNRTTEIENCEANVKMLRNVLRSV-IPNAPNLRHICLQT 147
              K S   +  + + ++  +     E + CE N  MLRN ++++ + +   LR + L T
Sbjct: 67  IAKKFSLEGVKGINYAYFAAYKEENNEEKLCEVNGNMLRNFVQALELTSIQTLRRVILTT 126

Query: 148 GTKHY---VGSFESIINKSSQRHDPPFTEDLPRLECPNFYYKQEDLLWEEIEQSQKKDLT 207
           G K Y   +G     + ++  R    F+        PNFYY QED+L +E    +K D T
Sbjct: 127 GLKFYGLHLGEVRLPMIETDIRVPETFSGT------PNFYYVQEDIL-KEFSNGKKWDYT 186

Query: 208 WAVIRPNLIFGFSPFSLMNVVGTLCVYAAICKHEGRPLKFPGNKLAWEDFQVASDADLIA 267
            A+  PN I G S  S MN   T+ +YA +C+    P +FPGN+  +  F   S + LIA
Sbjct: 187 IAM--PNDICGVSKGSYMNEAFTIALYALVCRELHEPFRFPGNEKFYLGFDDISYSKLIA 246

Query: 268 EQHIWTAVDPYSKNEAFNCNNGDVFKWKHFWKVLAEQFNIE------------------- 327
           +  +W         E FN  NGD+  W   W  +AE F +E                   
Sbjct: 247 DFQLWMTFKAECSEEKFNIVNGDIHSWSRTWPKIAEYFGVEVPKNQFATDFTLSTEVTLS 306

Query: 328 ----------EYGFDEEGESLTLVDL-------MKDKSDVWDEIVKENQLQQTKLEEIGT 387
                     E G      S  +  +        K   D W  I +  +L    L E+GT
Sbjct: 307 TPSPINLYEKELGIKHTPNSKIINQISLQQWVKQKKVQDAWRTIAEREKLNAHAL-EVGT 366

Query: 388 WWFVDSIFSISGN-IDSMNKSKEHGFLGFRNSKNSFISWIDKIKAFKIVP 395
           W F D +F  + N I SM+K+++ G+  + ++ + F    D++K  K +P
Sbjct: 367 WAFCDFLFGRTYNVISSMSKARKLGYTDYYDTFDGFKETFDELKKQKQIP 403

[Close]
BLAST of Cucsa.140700 vs. TrEMBL
Match: A0A0A0L8Y0_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_3G610790 PE=4 SV=1)

HSP 1 Score: 827.0 bits (2135), Expect = 9.6e-237
Identity = 393/394 (99.75%), Postives = 394/394 (100.00%), Query Frame = 1 

Query: 1   MSWWWAGAIGAAKKKFEEDEPPRSFESVALVIGVTGIVGNSLAEILPLSDTPGGPWKVYG 60
           MSWWWAGAIGAAKKKFEEDEPPRSFESVALVIGVTGIVGNSLAEILPLSDTPGGPWKVYG
Sbjct: 1   MSWWWAGAIGAAKKKFEEDEPPRSFESVALVIGVTGIVGNSLAEILPLSDTPGGPWKVYG 60

Query: 61  VARRPRPAWNADHPIEYIQCDVSDPQDAETKLSQLADVTHLFYVTWTNRTTEIENCEANV 120
           VARRPRPAWNADHPIEYIQCDVSDPQDAETKLSQLADVTHLFYVTWTNRTTEIENCEANV
Sbjct: 61  VARRPRPAWNADHPIEYIQCDVSDPQDAETKLSQLADVTHLFYVTWTNRTTEIENCEANV 120

Query: 121 KMLRNVLRSVIPNAPNLRHICLQTGTKHYVGSFESIINKSSQRHDPPFTEDLPRLECPNF 180
           KMLRNVLRSVIPNAPNLRHICLQTGTKHYVGSFESIINKSSQRHDPPFTEDLPRLECPNF
Sbjct: 121 KMLRNVLRSVIPNAPNLRHICLQTGTKHYVGSFESIINKSSQRHDPPFTEDLPRLECPNF 180

Query: 181 YYKQEDLLWEEIEQSQKKDLTWAVIRPNLIFGFSPFSLMNVVGTLCVYAAICKHEGRPLK 240
           YYKQEDLLWEEIEQSQKKDLTWAVIRPNLIFGFSPFSLMNVVGTLCVYAAICKHEGRPLK
Sbjct: 181 YYKQEDLLWEEIEQSQKKDLTWAVIRPNLIFGFSPFSLMNVVGTLCVYAAICKHEGRPLK 240

Query: 241 FPGNKLAWEDFQVASDADLIAEQHIWTAVDPYSKNEAFNCNNGDVFKWKHFWKVLAEQFN 300
           FPGNKLAWEDFQVASDADLIAEQHIWTAVDPYSKNEAFNCNNGDVFKWKHFWKVLAEQFN
Sbjct: 241 FPGNKLAWEDFQVASDADLIAEQHIWTAVDPYSKNEAFNCNNGDVFKWKHFWKVLAEQFN 300

Query: 301 IEEYGFDEEGESLTLVDLMKDKSDVWDEIVKENQLQQTKLEEIGTWWFVDSIFSISGNID 360
           IEEYGFDEEGESLTLVDLMKDKSDVWDEIVKENQLQQTKLEEIGTWWFVDSIFS+SGNID
Sbjct: 301 IEEYGFDEEGESLTLVDLMKDKSDVWDEIVKENQLQQTKLEEIGTWWFVDSIFSMSGNID 360

Query: 361 SMNKSKEHGFLGFRNSKNSFISWIDKIKAFKIVP 395
           SMNKSKEHGFLGFRNSKNSFISWIDKIKAFKIVP
Sbjct: 361 SMNKSKEHGFLGFRNSKNSFISWIDKIKAFKIVP 394

[Close]
BLAST of Cucsa.140700 vs. TrEMBL
Match: A0A0B5CYJ8_CUCPE (Putative progesterone 5-beta-reductase OS=Cucurbita pepo PE=2 SV=1)

HSP 1 Score: 711.1 bits (1834), Expect = 7.7e-202
Identity = 335/394 (85.03%), Postives = 358/394 (90.86%), Query Frame = 1 

Query: 1   MSWWWAGAIGAAKKKFEEDEPPRSFESVALVIGVTGIVGNSLAEILPLSDTPGGPWKVYG 60
           M WWWAGAIGAAKKKFEEDE PRSFESVALVIGVTGIVGNSLAEILPLSDTPGGPWKVYG
Sbjct: 1   MRWWWAGAIGAAKKKFEEDEQPRSFESVALVIGVTGIVGNSLAEILPLSDTPGGPWKVYG 60

Query: 61  VARRPRPAWNADHPIEYIQCDVSDPQDAETKLSQLADVTHLFYVTWTNRTTEIENCEANV 120
           VARRPRP WNADH IEYIQCDVSD +DAE KLS+L DVTH+FYVTWTN  TEIENCE N 
Sbjct: 61  VARRPRPNWNADHLIEYIQCDVSDLEDAEAKLSKLTDVTHVFYVTWTNLPTEIENCEVNG 120

Query: 121 KMLRNVLRSVIPNAPNLRHICLQTGTKHYVGSFESIINKSSQRHDPPFTEDLPRLECPNF 180
           KMLRNVLRSVIPNAPNLRHICLQTGTKHY+GSFE+II+  +QRHDPPFTED+PRL+ PNF
Sbjct: 121 KMLRNVLRSVIPNAPNLRHICLQTGTKHYIGSFENIIDNPTQRHDPPFTEDMPRLDTPNF 180

Query: 181 YYKQEDLLWEEIEQSQKKDLTWAVIRPNLIFGFSPFSLMNVVGTLCVYAAICKHEGRPLK 240
           YY QEDLL+EEIE+SQKKDLTWAVIRPN IFGFSPFSLMN+VGTLC YAAICKHEGRPLK
Sbjct: 181 YYTQEDLLFEEIEKSQKKDLTWAVIRPNTIFGFSPFSLMNIVGTLCTYAAICKHEGRPLK 240

Query: 241 FPGNKLAWEDFQVASDADLIAEQHIWTAVDPYSKNEAFNCNNGDVFKWKHFWKVLAEQFN 300
           FPG K AWE + V SDADLIAEQHIWTAVDPY+KNEAFNC+NGDVFKWKHFWKVLAEQFN
Sbjct: 241 FPGTKSAWEGYSVVSDADLIAEQHIWTAVDPYAKNEAFNCSNGDVFKWKHFWKVLAEQFN 300

Query: 301 IEEYGFDEEGESLTLVDLMKDKSDVWDEIVKENQLQQTKLEEIGTWWFVDSIFSISGNID 360
           IE+YGF EEGE L L +LMK K++VW+EIVKENQLQ TKLE++  WW  D   S SGNID
Sbjct: 301 IEDYGF-EEGERLPLRELMKGKNEVWEEIVKENQLQPTKLEDVANWWHTDIALSASGNID 360

Query: 361 SMNKSKEHGFLGFRNSKNSFISWIDKIKAFKIVP 395
           SMNKSKEHGFLGFRNSKNSFISWIDK+KAFKIVP
Sbjct: 361 SMNKSKEHGFLGFRNSKNSFISWIDKVKAFKIVP 393

[Close]
BLAST of Cucsa.140700 vs. TrEMBL
Match: V4MPF0_EUTSA (Uncharacterized protein OS=Eutrema salsugineum GN=EUTSA_v10025425mg PE=4 SV=1)

HSP 1 Score: 649.4 bits (1674), Expect = 2.7e-183
Identity = 300/394 (76.14%), Postives = 346/394 (87.82%), Query Frame = 1 

Query: 1   MSWWWAGAIGAAKKKFEEDEPPRSFESVALVIGVTGIVGNSLAEILPLSDTPGGPWKVYG 60
           MSWWWAGAIGAAKKK +EDEP +S+ESVAL+IGVTGIVGNSLAEILPLSDTPGGPWKVYG
Sbjct: 1   MSWWWAGAIGAAKKKLDEDEPTQSYESVALIIGVTGIVGNSLAEILPLSDTPGGPWKVYG 60

Query: 61  VARRPRPAWNADHPIEYIQCDVSDPQDAETKLSQLADVTHLFYVTWTNRTTEIENCEANV 120
           VARRPRP+WNADHPI+YIQCDVSD ++A +KLS+L DVTH+FYVTWTNR++E ENC+AN 
Sbjct: 61  VARRPRPSWNADHPIDYIQCDVSDAEEARSKLSRLTDVTHVFYVTWTNRSSETENCDANG 120

Query: 121 KMLRNVLRSVIPNAPNLRHICLQTGTKHYVGSFESIINKSSQRHDPPFTEDLPRLECPNF 180
            MLRNVLR+V+PNAPNLRH+CLQTGTKHY+G F++ + K SQRHDPPFTED+PRL+  NF
Sbjct: 121 SMLRNVLRAVLPNAPNLRHVCLQTGTKHYIGPFDN-LEKRSQRHDPPFTEDMPRLQIQNF 180

Query: 181 YYKQEDLLWEEIEQSQKKDLTWAVIRPNLIFGFSPFSLMNVVGTLCVYAAICKHEGRPLK 240
           YY  ED+L+EEI+  +K+ +TW+V RPN IFGFSP+SLMN+VGTLCVYAAICKHEG  L 
Sbjct: 181 YYTLEDVLFEEIK--KKESVTWSVHRPNTIFGFSPYSLMNIVGTLCVYAAICKHEGSKLV 240

Query: 241 FPGNKLAWEDFQVASDADLIAEQHIWTAVDPYSKNEAFNCNNGDVFKWKHFWKVLAEQFN 300
           FPG+K AWE F  ASDADL+AEQ IW AVDPY+KNEAFNCNN D+FKWKH WKVLAEQF 
Sbjct: 241 FPGSKKAWEGFTAASDADLVAEQQIWAAVDPYAKNEAFNCNNADMFKWKHLWKVLAEQFG 300

Query: 301 IEEYGFDEEGESLTLVDLMKDKSDVWDEIVKENQLQQTKLEEIGTWWFVDSIFSISGNID 360
           IEEYGF EEG++L LV++MK K  VW+E+VKENQLQ+ KL+E+G WWF D I  + G ID
Sbjct: 301 IEEYGF-EEGKNLGLVEMMKGKERVWEEMVKENQLQEKKLDEVGVWWFADVILGVEGMID 360

Query: 361 SMNKSKEHGFLGFRNSKNSFISWIDKIKAFKIVP 395
           SMNKSKEHGFLGFRNS NSFISWIDK KAFKIVP
Sbjct: 361 SMNKSKEHGFLGFRNSNNSFISWIDKYKAFKIVP 390

[Close]
BLAST of Cucsa.140700 vs. TrEMBL
Match: V7AWY5_PHAVU (Uncharacterized protein OS=Phaseolus vulgaris GN=PHAVU_009G070100g PE=4 SV=1)

HSP 1 Score: 645.6 bits (1664), Expect = 4.0e-182
Identity = 305/394 (77.41%), Postives = 339/394 (86.04%), Query Frame = 1 

Query: 1   MSWWWAGAIGAAKKKFEEDEPPRSFESVALVIGVTGIVGNSLAEILPLSDTPGGPWKVYG 60
           MSWWWAGAIGAAKKKFEEDEPPRSFESV LVIGVTGIVGNSLAEILPL DTPGGPWKVYG
Sbjct: 1   MSWWWAGAIGAAKKKFEEDEPPRSFESVGLVIGVTGIVGNSLAEILPLHDTPGGPWKVYG 60

Query: 61  VARRPRPAWNADHPIEYIQCDVSDPQDAETKLSQLADVTHLFYVTWTNRTTEIENCEANV 120
           VARRPRP+WNADHPIEY+QCDVSDP DAE+KLS L DVTH+F+V+WTNR TE ENCE N 
Sbjct: 61  VARRPRPSWNADHPIEYVQCDVSDPADAESKLSVLTDVTHVFFVSWTNRPTEAENCEVNG 120

Query: 121 KMLRNVLRSVIPNAPNLRHICLQTGTKHYVGSFESIINKSSQRHDPPFTEDLPRLECPNF 180
            ML+NVLR+VIPNAPNLRH+ LQTGTKHY+GSFESI     Q H+PPFTEDLPRL+ PNF
Sbjct: 121 AMLQNVLRAVIPNAPNLRHVSLQTGTKHYLGSFESI--GKIQSHEPPFTEDLPRLDTPNF 180

Query: 181 YYKQEDLLWEEIEQSQKKDLTWAVIRPNLIFGFSPFSLMNVVGTLCVYAAICKHEGRPLK 240
           YY QED+L+E  E ++K+ LTW+V RP  IFGFSP+SLMN+VG++CVYAAICKHEG PL+
Sbjct: 181 YYNQEDILFE--ESAKKEGLTWSVHRPGTIFGFSPYSLMNLVGSVCVYAAICKHEGVPLR 240

Query: 241 FPGNKLAWEDFQVASDADLIAEQHIWTAVDPYSKNEAFNCNNGDVFKWKHFWKVLAEQFN 300
           FPG K AWE +  +SDADLIAEQHIW AVDP ++NEAFNC+NGDVFKWKH WKVLAEQF 
Sbjct: 241 FPGTKGAWEGYSFSSDADLIAEQHIWAAVDPNARNEAFNCSNGDVFKWKHLWKVLAEQFG 300

Query: 301 IEEYGFDEEGESLTLVDLMKDKSDVWDEIVKENQLQQTKLEEIGTWWFVDSIFSISGNID 360
           IEEYGF EEG  L L ++MKDK  VWDEIV  NQL  TKLEE+G WWFVD IFS  G +D
Sbjct: 301 IEEYGF-EEGSHLKLSEVMKDKGPVWDEIVSANQLLPTKLEEVGDWWFVDIIFSAEGVLD 360

Query: 361 SMNKSKEHGFLGFRNSKNSFISWIDKIKAFKIVP 395
           SMNK+KEHGFLGFRNSKNS ISWIDK K +KIVP
Sbjct: 361 SMNKAKEHGFLGFRNSKNSLISWIDKCKGYKIVP 389

[Close]
BLAST of Cucsa.140700 vs. TrEMBL
Match: D7M8W5_ARALL (Putative uncharacterized protein OS=Arabidopsis lyrata subsp. lyrata GN=ARALYDRAFT_914168 PE=4 SV=1)

HSP 1 Score: 645.2 bits (1663), Expect = 5.2e-182
Identity = 299/394 (75.89%), Postives = 338/394 (85.79%), Query Frame = 1 

Query: 1   MSWWWAGAIGAAKKKFEEDEPPRSFESVALVIGVTGIVGNSLAEILPLSDTPGGPWKVYG 60
           MSWWWAGAIGAAKKK ++DEP +S+ESVAL+IGVTGIVGNSLAEILPLSDTPGGPWKVYG
Sbjct: 1   MSWWWAGAIGAAKKKLDDDEPSQSYESVALIIGVTGIVGNSLAEILPLSDTPGGPWKVYG 60

Query: 61  VARRPRPAWNADHPIEYIQCDVSDPQDAETKLSQLADVTHLFYVTWTNRTTEIENCEANV 120
           VARRPRP WNADHPI+YIQCDVSD +DA +KLS L DVTH+FYVTWTNR +E ENCEAN 
Sbjct: 61  VARRPRPTWNADHPIDYIQCDVSDAEDARSKLSPLTDVTHVFYVTWTNRESESENCEANG 120

Query: 121 KMLRNVLRSVIPNAPNLRHICLQTGTKHYVGSFESIINKSSQRHDPPFTEDLPRLECPNF 180
            MLRNVLR+++PNAPNLRH+CLQTGTKHYVG F    N    RHDPPFTED+PRL+  NF
Sbjct: 121 SMLRNVLRAIVPNAPNLRHVCLQTGTKHYVGPFS---NLDGPRHDPPFTEDMPRLQIQNF 180

Query: 181 YYKQEDLLWEEIEQSQKKDLTWAVIRPNLIFGFSPFSLMNVVGTLCVYAAICKHEGRPLK 240
           YY QED+L++EI++ +   +TW + RPN IFGFSP+SLMN+VGTLCVYAAICKHEG PL 
Sbjct: 181 YYTQEDVLFDEIKKIE--TVTWTIHRPNTIFGFSPYSLMNIVGTLCVYAAICKHEGSPLL 240

Query: 241 FPGNKLAWEDFQVASDADLIAEQHIWTAVDPYSKNEAFNCNNGDVFKWKHFWKVLAEQFN 300
           FPG+K AWE F  ASDADLIAEQ IW AVDPY+KNEAFNCNN D+FKWKH WK+LAEQF 
Sbjct: 241 FPGSKKAWEGFTTASDADLIAEQQIWAAVDPYAKNEAFNCNNADIFKWKHLWKILAEQFG 300

Query: 301 IEEYGFDEEGESLTLVDLMKDKSDVWDEIVKENQLQQTKLEEIGTWWFVDSIFSISGNID 360
           IEEYGF EEG++L LV++MK K  VW+E+VKENQLQ+ KLEE+G WWF D I  + G ID
Sbjct: 301 IEEYGF-EEGKNLGLVEMMKGKERVWEEMVKENQLQEKKLEEVGVWWFADVILGVDGLID 360

Query: 361 SMNKSKEHGFLGFRNSKNSFISWIDKIKAFKIVP 395
           SMNKSKEHGFLGFRNS NSFISWIDK KAFKIVP
Sbjct: 361 SMNKSKEHGFLGFRNSNNSFISWIDKYKAFKIVP 388

[Close]
BLAST of Cucsa.140700 vs. TAIR10
Match: AT4G24220.1 (AT4G24220.1 NAD(P)-binding Rossmann-fold superfamily protein)

HSP 1 Score: 641.7 bits (1654), Expect = 2.9e-184
Identity = 298/394 (75.63%), Postives = 338/394 (85.79%), Query Frame = 1 

Query: 1   MSWWWAGAIGAAKKKFEEDEPPRSFESVALVIGVTGIVGNSLAEILPLSDTPGGPWKVYG 60
           MSWWWAGAIGAAKKK +EDEP +SFESVAL+IGVTGIVGNSLAEILPLSDTPGGPWKVYG
Sbjct: 1   MSWWWAGAIGAAKKKLDEDEPSQSFESVALIIGVTGIVGNSLAEILPLSDTPGGPWKVYG 60

Query: 61  VARRPRPAWNADHPIEYIQCDVSDPQDAETKLSQLADVTHLFYVTWTNRTTEIENCEANV 120
           VARRPRP WNADHPI+YIQCDVSD +D  +KLS L DVTH+FYVTWTNR +E ENCEAN 
Sbjct: 61  VARRPRPTWNADHPIDYIQCDVSDAEDTRSKLSPLTDVTHVFYVTWTNRESESENCEANG 120

Query: 121 KMLRNVLRSVIPNAPNLRHICLQTGTKHYVGSFESIINKSSQRHDPPFTEDLPRLECPNF 180
            MLRNVL+++IP APNLRH+CLQTGTKHY+G F    N    RHDPPFTED+PRL+  NF
Sbjct: 121 SMLRNVLQAIIPYAPNLRHVCLQTGTKHYLGPF---TNVDGPRHDPPFTEDMPRLQIQNF 180

Query: 181 YYKQEDLLWEEIEQSQKKDLTWAVIRPNLIFGFSPFSLMNVVGTLCVYAAICKHEGRPLK 240
           YY QED+L+EEI++ +   +TW++ RPN+IFGFSP+SLMN+VGTLCVYAAICKHEG PL 
Sbjct: 181 YYTQEDILFEEIKKIE--TVTWSIHRPNMIFGFSPYSLMNIVGTLCVYAAICKHEGSPLL 240

Query: 241 FPGNKLAWEDFQVASDADLIAEQHIWTAVDPYSKNEAFNCNNGDVFKWKHFWKVLAEQFN 300
           FPG+K AWE F  ASDADLIAEQ IW AVDPY+KNEAFNCNN D+FKWKH WK+LAEQF 
Sbjct: 241 FPGSKKAWEGFMTASDADLIAEQQIWAAVDPYAKNEAFNCNNADIFKWKHLWKILAEQFG 300

Query: 301 IEEYGFDEEGESLTLVDLMKDKSDVWDEIVKENQLQQTKLEEIGTWWFVDSIFSISGNID 360
           IEEYGF EEG++L LV++MK K  VW+E+VKENQLQ+ KLEE+G WWF D I  + G ID
Sbjct: 301 IEEYGF-EEGKNLGLVEMMKGKERVWEEMVKENQLQEKKLEEVGVWWFADVILGVEGMID 360

Query: 361 SMNKSKEHGFLGFRNSKNSFISWIDKIKAFKIVP 395
           SMNKSKE+GFLGFRNS NSFISWIDK KAFKIVP
Sbjct: 361 SMNKSKEYGFLGFRNSNNSFISWIDKYKAFKIVP 388

[Close]
BLAST of Cucsa.140700 vs. TAIR10
Match: AT5G58750.1 (AT5G58750.1 NAD(P)-binding Rossmann-fold superfamily protein)

HSP 1 Score: 227.3 bits (578), Expect = 1.7e-59
Identity = 135/377 (35.81%), Postives = 206/377 (54.64%), Query Frame = 1 

Query: 26  ESVALVIGVTGIVGNSLAEILPLSDTPGGPWKVYGVARRPRPAWNADHPIEYIQCDVSDP 85
           E+VAL+ GVTG+VG  + + L +S  PG  W++YGVAR P    +      +I CD+ + 
Sbjct: 16  ENVALIFGVTGLVGREIVKTLLMSK-PG--WRIYGVARNPEIN-SMTKMYNFISCDLLNA 75

Query: 86  QDAETKLSQLAD-VTHLFYVTWTNR--TTEIENCEANVKMLRNVLRSVIPNAPNLRHICL 145
            + + +LS L D V+H+F+VTW+        E C  N  ML N L +++PNA  L+H  L
Sbjct: 76  SETKQRLSPLQDIVSHVFWVTWSGEFPLDTDECCVQNKTMLMNALDAILPNAKRLKHFSL 135

Query: 146 QTGTKHYVGSFESIINKSSQRHDPPFTEDLPRLEC-PNFYYKQEDLLWEEIEQSQKKDLT 205
           QTG KHYV   E  + +        ++E+ PR     NFYY  EDLL E+I +S    + 
Sbjct: 136 QTGMKHYVSLVEETMARGEGSSLYYYSEECPRKSSGKNFYYVLEDLLKEKITRSS---VV 195

Query: 206 WAVIRPNLIFGFSPFSLMNVVGTLCVYAAICKHEGRPLKFPGNKLAWEDFQV-ASDADLI 265
           W+V RP L+ G S  +L N +G+LCVY A+CK+   P  F G +  WE+  +  SD++L+
Sbjct: 196 WSVQRPGLLMGSSSRTLYNFMGSLCVYGAMCKYLNLPFVFGGTRECWEESYIDGSDSNLV 255

Query: 266 AEQHIWTAVD--PYSKNEAFNCNNGDVFKWKHFWKVLAEQFNIEEYGFDEEGESLTLVDL 325
           AEQHI+ A       K EAFN  NG  F WK  W  + ++  ++        E       
Sbjct: 256 AEQHIFAATSGKVREKGEAFNAINGVGFTWKEIWPEIGKKLGVQVNETTMFDEGFWFGRE 315

Query: 326 MKDKSDVWDEIVKENQLQQTKLEEIGTWWFVDSIFSISGN-IDSMNKSKEHGFLGFRNSK 385
           M ++  VWDEIV + +L +T++E++  W+F+D++F      +    K    GF     + 
Sbjct: 316 MVERKHVWDEIVVKEKLVRTEIEDLANWYFLDALFRCPFKLLGKREKVDRFGFKRKYRTL 375

Query: 386 NSFISWIDKIKAFKIVP 395
           +S + WID ++  K++P
Sbjct: 376 DSVLYWIDVMRDEKLIP 385

[Close]
BLAST of Cucsa.140700 vs. NCBI nr
Match: gi|449468584|ref|XP_004152001.1| (PREDICTED: 3-oxo-Delta(4,5)-steroid 5-beta-reductase [Cucumis sativus])

HSP 1 Score: 827.0 bits (2135), Expect = 1.4e-236
Identity = 393/394 (99.75%), Postives = 394/394 (100.00%), Query Frame = 1 

Query: 1   MSWWWAGAIGAAKKKFEEDEPPRSFESVALVIGVTGIVGNSLAEILPLSDTPGGPWKVYG 60
           MSWWWAGAIGAAKKKFEEDEPPRSFESVALVIGVTGIVGNSLAEILPLSDTPGGPWKVYG
Sbjct: 1   MSWWWAGAIGAAKKKFEEDEPPRSFESVALVIGVTGIVGNSLAEILPLSDTPGGPWKVYG 60

Query: 61  VARRPRPAWNADHPIEYIQCDVSDPQDAETKLSQLADVTHLFYVTWTNRTTEIENCEANV 120
           VARRPRPAWNADHPIEYIQCDVSDPQDAETKLSQLADVTHLFYVTWTNRTTEIENCEANV
Sbjct: 61  VARRPRPAWNADHPIEYIQCDVSDPQDAETKLSQLADVTHLFYVTWTNRTTEIENCEANV 120

Query: 121 KMLRNVLRSVIPNAPNLRHICLQTGTKHYVGSFESIINKSSQRHDPPFTEDLPRLECPNF 180
           KMLRNVLRSVIPNAPNLRHICLQTGTKHYVGSFESIINKSSQRHDPPFTEDLPRLECPNF
Sbjct: 121 KMLRNVLRSVIPNAPNLRHICLQTGTKHYVGSFESIINKSSQRHDPPFTEDLPRLECPNF 180

Query: 181 YYKQEDLLWEEIEQSQKKDLTWAVIRPNLIFGFSPFSLMNVVGTLCVYAAICKHEGRPLK 240
           YYKQEDLLWEEIEQSQKKDLTWAVIRPNLIFGFSPFSLMNVVGTLCVYAAICKHEGRPLK
Sbjct: 181 YYKQEDLLWEEIEQSQKKDLTWAVIRPNLIFGFSPFSLMNVVGTLCVYAAICKHEGRPLK 240

Query: 241 FPGNKLAWEDFQVASDADLIAEQHIWTAVDPYSKNEAFNCNNGDVFKWKHFWKVLAEQFN 300
           FPGNKLAWEDFQVASDADLIAEQHIWTAVDPYSKNEAFNCNNGDVFKWKHFWKVLAEQFN
Sbjct: 241 FPGNKLAWEDFQVASDADLIAEQHIWTAVDPYSKNEAFNCNNGDVFKWKHFWKVLAEQFN 300

Query: 301 IEEYGFDEEGESLTLVDLMKDKSDVWDEIVKENQLQQTKLEEIGTWWFVDSIFSISGNID 360
           IEEYGFDEEGESLTLVDLMKDKSDVWDEIVKENQLQQTKLEEIGTWWFVDSIFS+SGNID
Sbjct: 301 IEEYGFDEEGESLTLVDLMKDKSDVWDEIVKENQLQQTKLEEIGTWWFVDSIFSMSGNID 360

Query: 361 SMNKSKEHGFLGFRNSKNSFISWIDKIKAFKIVP 395
           SMNKSKEHGFLGFRNSKNSFISWIDKIKAFKIVP
Sbjct: 361 SMNKSKEHGFLGFRNSKNSFISWIDKIKAFKIVP 394

[Close]
BLAST of Cucsa.140700 vs. NCBI nr
Match: gi|659093104|ref|XP_008447374.1| (PREDICTED: 3-oxo-Delta(4,5)-steroid 5-beta-reductase [Cucumis melo])

HSP 1 Score: 793.1 bits (2047), Expect = 2.2e-226
Identity = 375/394 (95.18%), Postives = 385/394 (97.72%), Query Frame = 1 

Query: 1   MSWWWAGAIGAAKKKFEEDEPPRSFESVALVIGVTGIVGNSLAEILPLSDTPGGPWKVYG 60
           MSWWWAGAIGAAKKKFEEDEPPRSFESVALVIGVTGIVGNSLAEILPLSDTPGGPWKVYG
Sbjct: 1   MSWWWAGAIGAAKKKFEEDEPPRSFESVALVIGVTGIVGNSLAEILPLSDTPGGPWKVYG 60

Query: 61  VARRPRPAWNADHPIEYIQCDVSDPQDAETKLSQLADVTHLFYVTWTNRTTEIENCEANV 120
           VARRPRP WNADHPIEYIQCDVSDPQDAETKLSQLADVTHLFYVTWTNRT EI+NCEANV
Sbjct: 61  VARRPRPVWNADHPIEYIQCDVSDPQDAETKLSQLADVTHLFYVTWTNRTIEIDNCEANV 120

Query: 121 KMLRNVLRSVIPNAPNLRHICLQTGTKHYVGSFESIINKSSQRHDPPFTEDLPRLECPNF 180
           +MLRNVLRSVIPNAPNLRHICLQTGTKHYVGSFES+INKSSQRH+PPFTEDLPRLECPNF
Sbjct: 121 EMLRNVLRSVIPNAPNLRHICLQTGTKHYVGSFESVINKSSQRHEPPFTEDLPRLECPNF 180

Query: 181 YYKQEDLLWEEIEQSQKKDLTWAVIRPNLIFGFSPFSLMNVVGTLCVYAAICKHEGRPLK 240
           YYKQEDLL+EEIEQSQKKDLTWAVIRPN+IFGFSPFSLMN+VGTLCVYAAICKHEGRPLK
Sbjct: 181 YYKQEDLLFEEIEQSQKKDLTWAVIRPNVIFGFSPFSLMNIVGTLCVYAAICKHEGRPLK 240

Query: 241 FPGNKLAWEDFQVASDADLIAEQHIWTAVDPYSKNEAFNCNNGDVFKWKHFWKVLAEQFN 300
           FPG KLAWEDFQ ASDADL+AEQHIWTAVDPYSKNEAFNCNNGDVFKWKHFWKVLAEQFN
Sbjct: 241 FPGTKLAWEDFQAASDADLVAEQHIWTAVDPYSKNEAFNCNNGDVFKWKHFWKVLAEQFN 300

Query: 301 IEEYGFDEEGESLTLVDLMKDKSDVWDEIVKENQLQQTKLEEIGTWWFVDSIFSISGNID 360
           IEEYGF EEGE LTLVDLMKDKSDVWDEIVKENQLQ TKLEEIG WW+VD IFS+SGNID
Sbjct: 301 IEEYGF-EEGERLTLVDLMKDKSDVWDEIVKENQLQPTKLEEIGNWWYVDVIFSVSGNID 360

Query: 361 SMNKSKEHGFLGFRNSKNSFISWIDKIKAFKIVP 395
           SMNKSKEHGFLGFRNSKNSFISWIDKIKAFKIVP
Sbjct: 361 SMNKSKEHGFLGFRNSKNSFISWIDKIKAFKIVP 393

[Close]
BLAST of Cucsa.140700 vs. NCBI nr
Match: gi|748038959|gb|AJE29749.1| (putative progesterone 5-beta-reductase [Cucurbita pepo])

HSP 1 Score: 711.1 bits (1834), Expect = 1.1e-201
Identity = 335/394 (85.03%), Postives = 358/394 (90.86%), Query Frame = 1 

Query: 1   MSWWWAGAIGAAKKKFEEDEPPRSFESVALVIGVTGIVGNSLAEILPLSDTPGGPWKVYG 60
           M WWWAGAIGAAKKKFEEDE PRSFESVALVIGVTGIVGNSLAEILPLSDTPGGPWKVYG
Sbjct: 1   MRWWWAGAIGAAKKKFEEDEQPRSFESVALVIGVTGIVGNSLAEILPLSDTPGGPWKVYG 60

Query: 61  VARRPRPAWNADHPIEYIQCDVSDPQDAETKLSQLADVTHLFYVTWTNRTTEIENCEANV 120
           VARRPRP WNADH IEYIQCDVSD +DAE KLS+L DVTH+FYVTWTN  TEIENCE N 
Sbjct: 61  VARRPRPNWNADHLIEYIQCDVSDLEDAEAKLSKLTDVTHVFYVTWTNLPTEIENCEVNG 120

Query: 121 KMLRNVLRSVIPNAPNLRHICLQTGTKHYVGSFESIINKSSQRHDPPFTEDLPRLECPNF 180
           KMLRNVLRSVIPNAPNLRHICLQTGTKHY+GSFE+II+  +QRHDPPFTED+PRL+ PNF
Sbjct: 121 KMLRNVLRSVIPNAPNLRHICLQTGTKHYIGSFENIIDNPTQRHDPPFTEDMPRLDTPNF 180

Query: 181 YYKQEDLLWEEIEQSQKKDLTWAVIRPNLIFGFSPFSLMNVVGTLCVYAAICKHEGRPLK 240
           YY QEDLL+EEIE+SQKKDLTWAVIRPN IFGFSPFSLMN+VGTLC YAAICKHEGRPLK
Sbjct: 181 YYTQEDLLFEEIEKSQKKDLTWAVIRPNTIFGFSPFSLMNIVGTLCTYAAICKHEGRPLK 240

Query: 241 FPGNKLAWEDFQVASDADLIAEQHIWTAVDPYSKNEAFNCNNGDVFKWKHFWKVLAEQFN 300
           FPG K AWE + V SDADLIAEQHIWTAVDPY+KNEAFNC+NGDVFKWKHFWKVLAEQFN
Sbjct: 241 FPGTKSAWEGYSVVSDADLIAEQHIWTAVDPYAKNEAFNCSNGDVFKWKHFWKVLAEQFN 300

Query: 301 IEEYGFDEEGESLTLVDLMKDKSDVWDEIVKENQLQQTKLEEIGTWWFVDSIFSISGNID 360
           IE+YGF EEGE L L +LMK K++VW+EIVKENQLQ TKLE++  WW  D   S SGNID
Sbjct: 301 IEDYGF-EEGERLPLRELMKGKNEVWEEIVKENQLQPTKLEDVANWWHTDIALSASGNID 360

Query: 361 SMNKSKEHGFLGFRNSKNSFISWIDKIKAFKIVP 395
           SMNKSKEHGFLGFRNSKNSFISWIDK+KAFKIVP
Sbjct: 361 SMNKSKEHGFLGFRNSKNSFISWIDKVKAFKIVP 393

[Close]
BLAST of Cucsa.140700 vs. NCBI nr
Match: gi|567219662|ref|XP_006413460.1| (hypothetical protein EUTSA_v10025425mg [Eutrema salsugineum])

HSP 1 Score: 649.4 bits (1674), Expect = 3.9e-183
Identity = 300/394 (76.14%), Postives = 346/394 (87.82%), Query Frame = 1 

Query: 1   MSWWWAGAIGAAKKKFEEDEPPRSFESVALVIGVTGIVGNSLAEILPLSDTPGGPWKVYG 60
           MSWWWAGAIGAAKKK +EDEP +S+ESVAL+IGVTGIVGNSLAEILPLSDTPGGPWKVYG
Sbjct: 1   MSWWWAGAIGAAKKKLDEDEPTQSYESVALIIGVTGIVGNSLAEILPLSDTPGGPWKVYG 60

Query: 61  VARRPRPAWNADHPIEYIQCDVSDPQDAETKLSQLADVTHLFYVTWTNRTTEIENCEANV 120
           VARRPRP+WNADHPI+YIQCDVSD ++A +KLS+L DVTH+FYVTWTNR++E ENC+AN 
Sbjct: 61  VARRPRPSWNADHPIDYIQCDVSDAEEARSKLSRLTDVTHVFYVTWTNRSSETENCDANG 120

Query: 121 KMLRNVLRSVIPNAPNLRHICLQTGTKHYVGSFESIINKSSQRHDPPFTEDLPRLECPNF 180
            MLRNVLR+V+PNAPNLRH+CLQTGTKHY+G F++ + K SQRHDPPFTED+PRL+  NF
Sbjct: 121 SMLRNVLRAVLPNAPNLRHVCLQTGTKHYIGPFDN-LEKRSQRHDPPFTEDMPRLQIQNF 180

Query: 181 YYKQEDLLWEEIEQSQKKDLTWAVIRPNLIFGFSPFSLMNVVGTLCVYAAICKHEGRPLK 240
           YY  ED+L+EEI+  +K+ +TW+V RPN IFGFSP+SLMN+VGTLCVYAAICKHEG  L 
Sbjct: 181 YYTLEDVLFEEIK--KKESVTWSVHRPNTIFGFSPYSLMNIVGTLCVYAAICKHEGSKLV 240

Query: 241 FPGNKLAWEDFQVASDADLIAEQHIWTAVDPYSKNEAFNCNNGDVFKWKHFWKVLAEQFN 300
           FPG+K AWE F  ASDADL+AEQ IW AVDPY+KNEAFNCNN D+FKWKH WKVLAEQF 
Sbjct: 241 FPGSKKAWEGFTAASDADLVAEQQIWAAVDPYAKNEAFNCNNADMFKWKHLWKVLAEQFG 300

Query: 301 IEEYGFDEEGESLTLVDLMKDKSDVWDEIVKENQLQQTKLEEIGTWWFVDSIFSISGNID 360
           IEEYGF EEG++L LV++MK K  VW+E+VKENQLQ+ KL+E+G WWF D I  + G ID
Sbjct: 301 IEEYGF-EEGKNLGLVEMMKGKERVWEEMVKENQLQEKKLDEVGVWWFADVILGVEGMID 360

Query: 361 SMNKSKEHGFLGFRNSKNSFISWIDKIKAFKIVP 395
           SMNKSKEHGFLGFRNS NSFISWIDK KAFKIVP
Sbjct: 361 SMNKSKEHGFLGFRNSNNSFISWIDKYKAFKIVP 390

[Close]
BLAST of Cucsa.140700 vs. NCBI nr
Match: gi|694311984|ref|XP_009360382.1| (PREDICTED: 3-oxo-Delta(4,5)-steroid 5-beta-reductase-like [Pyrus x bretschneideri])

HSP 1 Score: 647.5 bits (1669), Expect = 1.5e-182
Identity = 300/394 (76.14%), Postives = 342/394 (86.80%), Query Frame = 1 

Query: 1   MSWWWAGAIGAAKKKFEEDEPPRSFESVALVIGVTGIVGNSLAEILPLSDTPGGPWKVYG 60
           MSWWWAGA+GAAKKKF+E+EPPR+ +SV LVIGVTGIVGNSLAEILPLSDTPGGPWKVYG
Sbjct: 1   MSWWWAGAVGAAKKKFDEEEPPRTPQSVGLVIGVTGIVGNSLAEILPLSDTPGGPWKVYG 60

Query: 61  VARRPRPAWNADHPIEYIQCDVSDPQDAETKLSQLADVTHLFYVTWTNRTTEIENCEANV 120
           VARRPRP WNADHP+EYIQCD+SDP DA+TKLS + DVTH+FYVTWTNR+TE ENCEAN 
Sbjct: 61  VARRPRPNWNADHPVEYIQCDISDPDDAKTKLSPITDVTHIFYVTWTNRSTEAENCEANA 120

Query: 121 KMLRNVLRSVIPNAPNLRHICLQTGTKHYVGSFESIINKSSQRHDPPFTEDLPRLECPNF 180
            MLRNVL SVIPNAP L+H+CLQTG KHYVG FE  +    Q H+PPFTEDLPRL+ PNF
Sbjct: 121 VMLRNVLNSVIPNAPKLQHVCLQTGAKHYVGPFE--LFGKIQPHEPPFTEDLPRLDAPNF 180

Query: 181 YYKQEDLLWEEIEQSQKKDLTWAVIRPNLIFGFSPFSLMNVVGTLCVYAAICKHEGRPLK 240
           YY  EDLL+ E+  ++K++LTW+V RP+ IFGFSP+S+MN++GTLCVYAAICKHEGRPLK
Sbjct: 181 YYTLEDLLFAEV--AKKENLTWSVHRPDAIFGFSPYSMMNIIGTLCVYAAICKHEGRPLK 240

Query: 241 FPGNKLAWEDFQVASDADLIAEQHIWTAVDPYSKNEAFNCNNGDVFKWKHFWKVLAEQFN 300
           FPG+K AW  + VASDADLIAEQHIW AVDPY+KNEAFN NNGDVFKWKHFWKVLAEQF 
Sbjct: 241 FPGSKAAWNCYSVASDADLIAEQHIWAAVDPYAKNEAFNINNGDVFKWKHFWKVLAEQFG 300

Query: 301 IEEYGFDEEGESLTLVDLMKDKSDVWDEIVKENQLQQTKLEEIGTWWFVDSIFSISGNID 360
           IEEYG DE G  L+LV+LMK K  VW+EIV+EN+LQ TKL E+G WWFVD   S  G + 
Sbjct: 301 IEEYGIDEGGGRLSLVELMKGKEKVWEEIVRENELQPTKLAEVGVWWFVDVWLSGEGLLC 360

Query: 361 SMNKSKEHGFLGFRNSKNSFISWIDKIKAFKIVP 395
           SMNKSKEHGFLGFRNS+NSF++WIDK+KAFKIVP
Sbjct: 361 SMNKSKEHGFLGFRNSRNSFVTWIDKMKAFKIVP 390

The following BLAST results are available for this feature:
BLAST of Cucsa.140700 vs. Swiss-Prot
Match NameE-valueIdentityDescription
VEP1_ARATH5.2e-18375.633-oxo-Delta(4,5)-steroid 5-beta-reductase OS=Arabidopsis thaliana GN=VEP1 PE=1 S... [more]
5BPOR_DIGLA2.9e-16269.043-oxo-Delta(4,5)-steroid 5-beta-reductase OS=Digitalis lanata PE=1 SV=1[more]
IRIS_CATRO8.1e-13658.94Iridoid synthase OS=Catharanthus roseus PE=1 SV=1[more]
YJ72_SCHPO3.5e-3026.83Uncharacterized protein C757.02c OS=Schizosaccharomyces pombe (strain 972 / ATCC... [more]
BLAST of Cucsa.140700 vs. TrEMBL
Match NameE-valueIdentityDescription
A0A0A0L8Y0_CUCSA9.6e-23799.75Uncharacterized protein OS=Cucumis sativus GN=Csa_3G610790 PE=4 SV=1[more]
A0A0B5CYJ8_CUCPE7.7e-20285.03Putative progesterone 5-beta-reductase OS=Cucurbita pepo PE=2 SV=1[more]
V4MPF0_EUTSA2.7e-18376.14Uncharacterized protein OS=Eutrema salsugineum GN=EUTSA_v10025425mg PE=4 SV=1[more]
V7AWY5_PHAVU4.0e-18277.41Uncharacterized protein OS=Phaseolus vulgaris GN=PHAVU_009G070100g PE=4 SV=1[more]
D7M8W5_ARALL5.2e-18275.89Putative uncharacterized protein OS=Arabidopsis lyrata subsp. lyrata GN=ARALYDRA... [more]
BLAST of Cucsa.140700 vs. TAIR10
Match NameE-valueIdentityDescription
AT4G24220.12.9e-18475.63 NAD(P)-binding Rossmann-fold superfamily protein[more]
AT5G58750.11.7e-5935.81 NAD(P)-binding Rossmann-fold superfamily protein[more]
BLAST of Cucsa.140700 vs. NCBI nr
Match NameE-valueIdentityDescription
gi|449468584|ref|XP_004152001.1|1.4e-23699.75PREDICTED: 3-oxo-Delta(4,5)-steroid 5-beta-reductase [Cucumis sativus][more]
gi|659093104|ref|XP_008447374.1|2.2e-22695.18PREDICTED: 3-oxo-Delta(4,5)-steroid 5-beta-reductase [Cucumis melo][more]
gi|748038959|gb|AJE29749.1|1.1e-20185.03putative progesterone 5-beta-reductase [Cucurbita pepo][more]
gi|567219662|ref|XP_006413460.1|3.9e-18376.14hypothetical protein EUTSA_v10025425mg [Eutrema salsugineum][more]
gi|694311984|ref|XP_009360382.1|1.5e-18276.14PREDICTED: 3-oxo-Delta(4,5)-steroid 5-beta-reductase-like [Pyrus x bretschneider... [more]
Annotated Terms
The following terms have been associated with this gene:
Vocabulary: INTERPRO
TermDefinition
IPR001509Epimerase_deHydtase
IPR016040NAD(P)-bd_dom
Vocabulary: Molecular Function
TermDefinition
GO:0003824catalytic activity
GO:0050662coenzyme binding
GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0008209 androgen metabolic process
biological_process GO:0006699 bile acid biosynthetic process
biological_process GO:0008207 C21-steroid hormone metabolic process
biological_process GO:0008210 estrogen metabolic process
biological_process GO:0055114 oxidation-reduction process
biological_process GO:0010051 xylem and phloem pattern formation
cellular_component GO:0005829 cytosol
molecular_function GO:0003854 3-beta-hydroxy-delta5-steroid dehydrogenase activity
molecular_function GO:0047568 3-oxo-5-beta-steroid 4-dehydrogenase activity
molecular_function GO:0050662 coenzyme binding
molecular_function GO:0035671 enone reductase activity
molecular_function GO:0003824 catalytic activity
Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Cucsa.140700.1Cucsa.140700.1mRNA


InterPro
Analysis Name: InterPro Annotations of cucumber (Gy14)
Date Performed: 2017-01-17
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001509NAD-dependent epimerase/dehydratase, N-terminal domainPFAMPF01370Epimerasecoord: 29..256
score: 2.
IPR016040NAD(P)-binding domainGENE3DG3DSA:3.40.50.720coord: 29..302
score: 2.1
IPR016040NAD(P)-binding domainunknownSSF51735NAD(P)-binding Rossmann-fold domainscoord: 28..300
score: 1.38
NoneNo IPR availablePANTHERPTHR32487FAMILY NOT NAMEDcoord: 2..394
score: 1.7E
NoneNo IPR availablePANTHERPTHR32487:SF03-OXO-DELTA(4,5)-STEROID 5-BETA-REDUCTASEcoord: 2..394
score: 1.7E

Synteny
The following gene(s) are orthologous to this gene:
GeneOrthologueOrganismBlock
Cucsa.140700Csa3G610790Cucumber (Chinese Long) v2cgycuB204
Cucsa.140700MELO3C012842Melon (DHL92) v3.5.1cgymeB234
Cucsa.140700CSPI03G26860Wild cucumber (PI 183967)cgycpiB207
Cucsa.140700MELO3C012842.2Melon (DHL92) v3.6.1cgymedB231
Cucsa.140700CsaV3_3G028370Cucumber (Chinese Long) v3cgycucB212
The following gene(s) are paralogous to this gene:

None

The following block(s) are covering this gene:
GeneOrganismBlock
Cucsa.140700Cucumber (Gy14) v1cgycgyB090
Cucsa.140700Cucurbita maxima (Rimu)cgycmaB0392
Cucsa.140700Cucurbita maxima (Rimu)cgycmaB0393
Cucsa.140700Cucurbita moschata (Rifu)cgycmoB0390
Cucsa.140700Cucurbita moschata (Rifu)cgycmoB0391
Cucsa.140700Cucurbita moschata (Rifu)cgycmoB0392
Cucsa.140700Wild cucumber (PI 183967)cgycpiB206
Cucsa.140700Cucumber (Chinese Long) v2cgycuB203
Cucsa.140700Melon (DHL92) v3.5.1cgymeB235
Cucsa.140700Watermelon (Charleston Gray)cgywcgB238
Cucsa.140700Watermelon (Charleston Gray)cgywcgB239
Cucsa.140700Watermelon (97103) v1cgywmB248
Cucsa.140700Watermelon (97103) v1cgywmB247
Cucsa.140700Watermelon (97103) v1cgywmB249
Cucsa.140700Cucurbita pepo (Zucchini)cgycpeB0379
Cucsa.140700Cucurbita pepo (Zucchini)cgycpeB0380
Cucsa.140700Cucurbita pepo (Zucchini)cgycpeB0381
Cucsa.140700Bottle gourd (USVL1VR-Ls)cgylsiB225
Cucsa.140700Bottle gourd (USVL1VR-Ls)cgylsiB226
Cucsa.140700Melon (DHL92) v3.6.1cgymedB232
Cucsa.140700Silver-seed gourdcarcgyB0275
Cucsa.140700Silver-seed gourdcarcgyB0843
Cucsa.140700Silver-seed gourdcarcgyB0884
Cucsa.140700Cucumber (Chinese Long) v3cgycucB211
Cucsa.140700Watermelon (97103) v2cgywmbB237
Cucsa.140700Watermelon (97103) v2cgywmbB238
Cucsa.140700Wax gourdcgywgoB276