Cucsa.133570 (gene) Cucumber (Gy14) v1

BLAST of Cucsa.133570 vs. Swiss-Prot
Match: EI2BE_DICDI (Translation initiation factor eIF-2B subunit epsilon OS=Dictyostelium discoideum GN=eif2b5 PE=3 SV=1)

HSP 1 Score: 454.5 bits (1168), Expect = 2.2e-126
Identity = 254/713 (35.62%), Postives = 414/713 (58.06%), Query Frame = 1

Query: 23  LQAVLLADSFTTKFRPITLERPKVLLPLVNVPMINYTLSWLESAGVEEVFVFCCANSKQV 82
           LQAV+L DSF  KF PITLE+P+ LLPLVN+P+++YTL +L ++GV+++FVFCCA++ Q+
Sbjct: 25  LQAVVLGDSFDRKFAPITLEKPRTLLPLVNIPLLDYTLEFLAASGVQQIFVFCCAHASQI 84

Query: 83  IDYLENSDWVSLPDFAVRTIESHNSISAGDALRLIYERNVIHGDFVLITGDTISNMSLTQ 142
            +Y+++S W  LP   V  +   N  + GDALR +Y+  VI  DF+LI+GD +SNM+L +
Sbjct: 85  KEYIQSSRWHDLPGVQVICMTGSNCRTTGDALRGVYDAQVIQSDFILISGDVVSNMNLQK 144

Query: 143 ALQEHKERKKKDSNAVMTMVVKRSKPSPITHQSRLGTDELFMAIDHNTKQLVYYEDRVDY 202
           ALQ HK+R++ D N +MTMV K++     TH++R   D+  +  + +T Q+V Y++    
Sbjct: 145 ALQIHKDRRELDKNNIMTMVYKQASS---THRTRSKQDDTVIWCNRDTMQVVCYDNSPSK 204

Query: 203 SKGIIHLDKSLIMDNSSISLHNDMQDCYIDICSPEVLSLFTDNFDYQHLRRHFVKGLLLD 262
            K  I ++  L   + SI +  D+ DC+IDICSPEVL+LF DNFD+  +R+ F+  +L  
Sbjct: 205 KKSSISVE--LFQKHPSIQMRYDLIDCHIDICSPEVLALFNDNFDFADIRKDFIHDILTS 264

Query: 263 DIMGYKIYTHEICSSYAARIDNYRSYDTVSKDIIQRWTYPLVPDVKFFGNSTYKLERQGM 322
           D++ YK+  + +   YAAR+ + R+Y +VSKDII RWT+P+VPD  F  NS+Y L RQ +
Sbjct: 265 DLLDYKLSAYVLQGEYAARVKDLRTYHSVSKDIIHRWTFPMVPDNNFMCNSSYSLSRQMI 324

Query: 323 YRASEILQARSAQVGPFTVIGDNSKIGDNTKITNSIIGHGCSIGSNVKIEGSYIWDNVTI 382
           Y+   +       +   TVIG  ++IG  + +++S IG  C IG NVKI GSYIWD+VTI
Sbjct: 325 YKEKNVKLLGDCLISDETVIGTQTEIGAGSIVSHSTIGRNCIIGKNVKINGSYIWDDVTI 384

Query: 383 EDGCKISNAIVCDGVVIKSGAELEPGVILSFKVVVGDQFTVPSYSKVSLFEQPTNQDSDE 442
           +D   I ++I+C+G +IKS + +  G I+ F V +G   T+  +SK+++ +   ++D +E
Sbjct: 385 QDNAIIDHSIICNGSIIKSSSIIGRGSIIGFNVYIGQSKTLEPFSKITMAQYNEDEDDEE 444

Query: 443 ELEYADNSSGIVEFADTTDKSNSALMSNPLEMQGWPASELGKGGLGYVWSICEGSVEEEW 502
            LE        +   D  + +N+    N  +   W                    +E E 
Sbjct: 445 LLEEYFKE---INLNDDNNNNNNNNNENN-KTNRW-------------------LMENEL 504

Query: 503 RHSVAPIPADKLEKVLHKAPDDEVELTQDANVLPPSG-ELKSDSYVSDGDDNESSRDDSI 562
            + + P   D +         D++E  +  +    SG ++K++    + DDN     DS+
Sbjct: 505 YNELVPRINDSIH--------DDIESDESGDEGDKSGGKIKNNK---NNDDNPIE-PDSV 564

Query: 563 HFEKEVEATFLRAVHENIEVANVILEVNSLRLSFNKVASDCAGAIFYSLMNFALDSSRGS 622
            F +EV  T  R + E + + N+ LE+N L+ ++++   DC  +I   L+  +  SS  +
Sbjct: 565 KFHREVGDTIRRGIIEKLPLENIQLEINGLKFAYDRDGLDCLTSILPVLLESSSSSSSTT 624

Query: 623 SS----ELLKNTTSIITKWKKLLKYYLTDLDEEIEVILKFEEICLESAKEFTPHFTKILW 682
            S    EL +     I+ +  LL  + ++ D  +++I K ++ C E+ K F   F  IL 
Sbjct: 625 DSVTPKELQQFIAGRISAFSPLLVKFSSE-DSMVDLIFKIQDFCDENEK-FKVVFQPILH 684

Query: 683 QLYDQEIIQEEAILKWDAEKKEADDSDKLFVKQAEKFIEWLKVASEEEEEEEE 731
           QLY+ ++I EEAI +W  E +  ++ D  ++K+ + FI+WLK A EE ++ ++
Sbjct: 685 QLYENDVISEEAIFEWAEEIEGDEEDDGFYLKKCKGFIDWLKSAEEESDDSDD 695

BLAST of Cucsa.133570 vs. Swiss-Prot
Match: EI2BE_CANAL (Translation initiation factor eIF-2B subunit epsilon OS=Candida albicans (strain SC5314 / ATCC MYA-2876) GN=GCD6 PE=3 SV=2)

HSP 1 Score: 407.9 bits (1047), Expect = 2.3e-112
Identity = 249/747 (33.33%), Postives = 399/747 (53.41%), Query Frame = 1

Query: 12  SDDHEELARVSLQAVLLADSFTTKFRPITLERPKVLLPLVNVPMINYTLSWLESAGVEEV 71
           S   ++L     QA++L DSF T+F P+T   P+ LLPL NVP+I YTL +L +AGV EV
Sbjct: 15  SKKSKDLVDERFQAIVLTDSFETRFMPLTAVHPRCLLPLANVPLIEYTLEFLANAGVNEV 74

Query: 72  FVFCCANSKQVIDYLENSDWVS-LPDFAVRTIESHNSISAGDALRLIYERNVIHGDFVLI 131
           ++ C A++ Q+ +Y+ENS W+     F+V TI S  S S GD +R +  R +I GDF+L+
Sbjct: 75  YLMCSAHADQIQEYIENSKWMGDNSPFSVTTIMSIESRSVGDTMRDLDNRGLIAGDFLLV 134

Query: 132 TGDTISNMSLTQALQEHKERKKKDSNAVMTMVVKRSKPSPITHQSRLGTDELFMAIDHNT 191
           +GD ++NM  ++ALQ HK++K +D + + TMV+ ++ P    H++R   D     +D  T
Sbjct: 135 SGDVVTNMDFSKALQFHKQKKAQDKDHIATMVLNQASP---LHRTRSQIDPAAFVLDKET 194

Query: 192 KQLVYYED--RVDYSKGIIHLDKSLIMD-NSSISLHNDMQDCYIDICSPEVLSLFTDNFD 251
            + ++Y+    V   K  I +D  L+ D    + + ND+ DC++DICSP V  +F +NFD
Sbjct: 195 NRCIFYQSIPPVSGKKTCISIDPELLEDFQGELQVRNDLIDCHVDICSPHVPQIFQENFD 254

Query: 252 YQHLRRHFVKGLLLDDIMGYKIYTH--EICSSYAARIDNYRSYDTVSKDIIQRWTYPLVP 311
           YQ+LR  F+KG+L  D++   IY +  +  S YAAR++++ +YD +S+DI+ RW YPLVP
Sbjct: 255 YQYLRSDFLKGVLTSDLLKKTIYAYISKDSSEYAARVESWSTYDAISQDILARWCYPLVP 314

Query: 312 DVKFFGNSTYKLERQGMYRASEILQARSAQVGPFTVIGDNSKIGDNTKITNSIIGHGCSI 371
           D      ++Y  E   +Y+  +I+ A+S ++G  T IG NS +G+ T+I NS+IG  C+I
Sbjct: 315 DSNLVEGNSYSYELNNIYKEDKIILAQSCKIGTSTSIGRNSSVGEGTQIKNSVIGRNCTI 374

Query: 372 GSNVKIEGSYIWDNVTIEDGCKISNAIVCDGVVIKSGAELEPGVILSFKVVVGDQFTVPS 431
           G NV IE SYIWDN  I+D   ++ +IV     I +   L PG ++ F V++GD   +P 
Sbjct: 375 GKNVVIENSYIWDNAVIKDNSVLNRSIVAADAQIGNNVTLSPGSVIGFNVIIGDDKVIP- 434

Query: 432 YSKVSLFEQP-----------TNQDSDEELEYAD-NSSGIVEFADTT---DKSNSALMSN 491
              V + E P              +S++E EY D N+  ++   D     +        +
Sbjct: 435 -HNVKIVETPIVTENEFGDFDDESNSEDENEYEDGNAVPVLAVKDVELVGETGKGFAYES 494

Query: 492 PLEMQGWPASELGKGGL--GYVWSICEGSVEEEWRHSVAPIPADKLEKVLHKAPDDEVEL 551
            +E       E    G   G ++ +   +V ++   S+A +   K++K  H+       L
Sbjct: 495 EIESGDEDDEEFVGNGTYSGIIYQMNSLNVSDD---SIASVSNKKVKKHSHRR-----RL 554

Query: 552 TQDANVLPPSGELKSDSYVSDGDDNESSRDDSIHFEKEVEATFLRAVHENIEVANVILEV 611
           + ++ +    G  +SD    +G++ E        F  E  AT  RA+  N ++   +LE+
Sbjct: 555 SMNSMISDNGGAFESD----EGEEEED-------FGVEGLATVTRAIENNHDIDTALLEL 614

Query: 612 NSLRLSFNKVASDCAGAIFYSLMNFALDSSRGSSSELLKNTTSIITKWKKLLKYYLTDLD 671
           N+LR+S N    D       +L+N  +D     +    +  T I TKW  + K  +   +
Sbjct: 615 NTLRMSMNVTYHDVRSVTTQALVNKIVDFITTGTLTPQEAATKIFTKWGIMFKRQVFSPE 674

Query: 672 EEIEVILKFEE--ICLESAKEFTPHFTKILWQLYDQEIIQEEAILKW--DAEKKEADDSD 731
           EE++++   EE    L+ A      F  +    YD E+++EE ILKW  D E  E     
Sbjct: 675 EEVDLLNIVEEKSSVLDKAYNQIVLFLGVK-SFYDMEVVEEENILKWWNDGENDEVR--- 729

BLAST of Cucsa.133570 vs. Swiss-Prot
Match: EI2BE_SCHPO (Probable translation initiation factor eIF-2B subunit epsilon OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) GN=tif225 PE=1 SV=1)

HSP 1 Score: 394.4 bits (1012), Expect = 2.7e-108
Identity = 232/720 (32.22%), Postives = 388/720 (53.89%), Query Frame = 1

Query: 17  ELARVSLQAVLLADSFTTKFRPITLERPKVLLPLVNVPMINYTLSWLESAGVEEVFVFCC 76
           E  + +LQA++L+DS+  +FRP+TL++P+ LLPL N P+I YT  +L  AGV+EV+VFCC
Sbjct: 12  EKPKHALQAIVLSDSYNYRFRPLTLDKPRCLLPLANTPLIEYTFEFLALAGVQEVYVFCC 71

Query: 77  ANSKQVIDYLENSDWVSLPD--FAVRTIESHNSISAGDALRLIYERNVIHGDFVLITGDT 136
           A++ Q+ +Y+E S W +LP   F+V TI S  S+S GDALR +  + +I  DF+L++GD 
Sbjct: 72  AHAGQIREYIEKSKW-NLPSSPFSVNTIVSRESLSVGDALRELDSKQLITSDFILVSGDV 131

Query: 137 ISNMSLTQALQEHKERKKKDSNAVMTMVVKRSKPSPITHQSRLGTDELFMAIDHNTKQLV 196
           +SN+ L + L+EH++R++ D NA+MTMVV+ + P    H++R  T+     ID  T Q V
Sbjct: 132 VSNVPLNEVLKEHRKRREDDKNAIMTMVVREASPF---HRTRARTESSVFVIDKKTSQCV 191

Query: 197 YYEDRVDYSKGIIHLDKSLIMDNSSISLHNDMQDCYIDICSPEVLSLFTDNFDYQHLRRH 256
           +Y+   +  K  + +D  +  ++  + + ND+ DC IDICS +V +LFT+NFDYQ +R+ 
Sbjct: 192 HYQAN-ERGKHYVSMDPEIFNEHEELEVRNDLIDCQIDICSNDVPALFTENFDYQDIRKD 251

Query: 257 FVKGLLLDDIMGYKIYTHEICSSYAARIDNYRSYDTVSKDIIQRWTYPLVPDVKFFGNST 316
           FV G+L  D++G KI+ H    +YAAR+ + ++YD +SKD++ RW YP VPD     N T
Sbjct: 252 FVYGVLTSDLLGKKIHCHVAKENYAARVRSLQTYDAISKDVLSRWVYPFVPDSNLL-NQT 311

Query: 317 YKLERQGMYRASEILQARSAQVGPFTVIGDNSKIGDNTKITNSIIGHGCSIGSNVKIEGS 376
           +  +R  +Y+  +++ ARS  +   T+IG  +K+GD + + N+IIG  C+IGSN  I+ +
Sbjct: 312 FSYQRHQIYKEEDVVLARSCIIKARTLIGAYTKVGDASVVANTIIGRNCTIGSNCSIDSA 371

Query: 377 YIWDNVTIEDGCKISNAIVCDGVVIKSGAELEPGVILSFKVVVGDQFTVPSYSKVSLFEQ 436
           ++W++V I D C+I                     IL+  V +G+  ++   + V+    
Sbjct: 372 FLWEDVVIGDNCRIGK------------------AILANSVKIGNNCSIEDGAIVA---- 431

Query: 437 PTNQDSDEELEYADNSSGIVEFADTTDKSNSALMSNPLEMQGW--PASELGKGGLGYVWS 496
                           +G+V   +T  + N  L +     QG     S +G GG G  + 
Sbjct: 432 ----------------AGVVIGDNTIIEKNKRLTTFESHSQGTLNDPSLVGIGGRGQEYH 491

Query: 497 ICEGSVEEEWRHSVAPIPADKLEKVLHKAPDDEVELT--QDANVLPPSGELKSDSYVSDG 556
             E S +E      + +     E  L  +   E   +  +D   +P S    S + ++  
Sbjct: 492 AEEDSDDEGEFMEASGLIESTNELHLSDSESSETSSSSEEDMEFIPFSARRDSANTINSE 551

Query: 557 DDNESSRDDSIHFEKEVEATFLRAVHENIEVANVILEVNSLRLSFNKVASDCAGAIFYSL 616
           D +E        F KE + +  RA  EN ++    LE+N+LR++ N    +   AI  +L
Sbjct: 552 DFDEGD------FNKEAQQSLERAFEENHQIDIAALELNTLRMAMNANYHEVRSAIVLAL 611

Query: 617 MNFALDSSRGSSSELLKNTTSIITKWKKLLKYYLTDLDEEIEVILKFEEICLESAKEFTP 676
           +   +         L K    ++T+W  LL       +E+++ +L  ++ C+  +   T 
Sbjct: 612 LRRIMHLDVSPKEALAK----VMTRWGPLLAKLTFSHEEQVDNVLTLQKYCVRLS--MTR 671

Query: 677 HFTKILWQLYDQEIIQEEAILKWDAEKKEAD-DSDKLFVKQAEKFIEWLKVASEEEEEEE 730
           HF ++L   Y  EI +E AI +W ++ + ++ +   L     ++F++WL  A  E E EE
Sbjct: 672 HFLQLLGYFYQLEIAEENAIQEWYSDPRSSEGELAALRDAGGKQFVDWLNTAESESESEE 675

BLAST of Cucsa.133570 vs. Swiss-Prot
Match: EI2BE_HUMAN (Translation initiation factor eIF-2B subunit epsilon OS=Homo sapiens GN=EIF2B5 PE=1 SV=3)

HSP 1 Score: 393.3 bits (1009), Expect = 5.9e-108
Identity = 238/723 (32.92%), Postives = 380/723 (52.56%), Query Frame = 1

Query: 16  EELARVSLQAVLLADSFTTKFRPITLERPKVLLPLVNVPMINYTLSWLESAGVEEVFVFC 75
           EE     LQAVL+ADSF  +F PI+ ++P+VLLPL NV +I+YTL +L + GV+E FVFC
Sbjct: 36  EEEPPPPLQAVLVADSFDRRFFPISKDQPRVLLPLANVALIDYTLEFLTATGVQETFVFC 95

Query: 76  CANSKQVIDYLENSDWVSLPDF-AVRTIESHNSISAGDALRLIYERNVIHGDFVLITGDT 135
           C  + Q+ ++L  S W        VR I S    S GD LR +  + ++  DF+L+ GD 
Sbjct: 96  CWKAAQIKEHLLKSKWCRPTSLNVVRIITSELYRSLGDVLRDVDAKALVRSDFLLVYGDV 155

Query: 136 ISNMSLTQALQEHKERKKKDSN-AVMTMVVKRSKPSPITHQSRLGTDELFMAIDHNTKQL 195
           ISN+++T+AL+EH+ R+K + N +VMTM+ K S PS   H +R   D + +A+D  T ++
Sbjct: 156 ISNINITRALEEHRLRRKLEKNVSVMTMIFKESSPS---HPTRCHEDNVVVAVDSTTNRV 215

Query: 196 VYYEDRVDYSKGIIHLDKSLIMDNSS-ISLHNDMQDCYIDICSPEVLSLFTDNFDYQHLR 255
           ++++      +    L  SL   +S  + +  D+ DC+I ICSP+V  LFTDNFDYQ  R
Sbjct: 216 LHFQKTQGLRRFAFPL--SLFQGSSDGVEVRYDLLDCHISICSPQVAQLFTDNFDYQ-TR 275

Query: 256 RHFVKGLLL-DDIMGYKIYTHEICSSYAARIDNYRSYDTVSKDIIQRWTYPLVPDVKFFG 315
             FV+GLL+ ++I+G +I+ H     Y AR+ N   Y  V  D+I+RW YPL P+  F  
Sbjct: 276 DDFVRGLLVNEEILGNQIHMHVTAKEYGARVSNLHMYSAVCADVIRRWVYPLTPEANFTD 335

Query: 316 NSTYKL--ERQGMYRASEILQARSAQVGPFTVIGDNSKIGDNTKITNSIIGHGCSIGSNV 375
           ++T      R  +YR  E+     + +    ++G  + IG N  ITNS+IG GC IG NV
Sbjct: 336 STTQSCTHSRHNIYRGPEVSLGHGSILEENVLLGSGTVIGSNCFITNSVIGPGCHIGDNV 395

Query: 376 KIEGSYIWDNVTIEDGCKISNAIVCDGVVIKSGAELEPGVILSFKVVVGDQFTVPSYSKV 435
            ++ +Y+W  V +  G +I  +++CD   +K    L+P  +L+ +VVVG   T+P  S +
Sbjct: 396 VLDQTYLWQGVRVAAGAQIHQSLLCDNAEVKERVTLKPRSVLTSQVVVGPNITLPEGSVI 455

Query: 436 SLFEQPTNQDSDEELEYADNSSGIVEFADTTDKSNSALMSNPLEMQGWPASELGKGGLGY 495
           SL      +D D+  E++D+S    E              + ++M+G+  +E+G  G GY
Sbjct: 456 SLHPPDAEEDEDDG-EFSDDSGADQE-------------KDKVKMKGYNPAEVGAAGKGY 515

Query: 496 VWSICEGSVEEEWRHSVAPIPADKLEKVLHKAPDDEVELTQDANVLPPSGELKSDSYVSD 555
           +W     ++EEE             E++       ++ + +++       E   DS   D
Sbjct: 516 LWKAAGMNMEEE-------------EELQQNLWGLKINMEEESE---SESEQSMDSEEPD 575

Query: 556 GDDNESSRDDSIHFEKEVEATFLRAVHENIEVANVILEVNSLRLSFNKVASDCAGAIFYS 615
                   DD   F+ EV  T  R   ENI   N++LE+NSL+ ++N    +    + + 
Sbjct: 576 SRGGSPQMDDIKVFQNEVLGTLQRGKEENISCDNLVLEINSLKYAYNISLKEVMQVLSHV 635

Query: 616 LMNFALD--SSRGSSSELLKNTTSIITKWKKLLKYYLTDLDEEIEVILKFEEICLESAKE 675
           ++ F L    S   SS        ++  W  + + Y+    + +E +   E+  LE  + 
Sbjct: 636 VLEFPLQQMDSPLDSSRYCALLLPLLKAWSPVFRNYIKRAADHLEALAAIEDFFLEH-EA 695

Query: 676 FTPHFTKILWQLYDQEIIQEEAILKWDAEKKEADDSDKLFV-KQAEKFIEWLKVASEEEE 730
                 K+L   Y  EI+ EE IL W +++   D   +L   +Q ++FI+WLK A EE  
Sbjct: 696 LGISMAKVLMAFYQLEILAEETILSWFSQRDTTDKGQQLRKNQQLQRFIQWLKEAEEESS 721

BLAST of Cucsa.133570 vs. Swiss-Prot
Match: EI2BE_RAT (Translation initiation factor eIF-2B subunit epsilon OS=Rattus norvegicus GN=Eif2b5 PE=1 SV=2)

HSP 1 Score: 386.7 bits (992), Expect = 5.5e-106
Identity = 234/727 (32.19%), Postives = 385/727 (52.96%), Query Frame = 1

Query: 16  EELARVSLQAVLLADSFTTKFRPITLERPKVLLPLVNVPMINYTLSWLESAGVEEVFVFC 75
           EE     LQAVL+ADSF  +F PI+ ++P+VLLPL NV +I+YTL +L + GV+E FVFC
Sbjct: 31  EEEPPPPLQAVLVADSFDRRFFPISKDQPRVLLPLANVALIDYTLEFLTATGVQETFVFC 90

Query: 76  CANSKQVIDYLENSDWVSLPDF-AVRTIESHNSISAGDALRLIYERNVIHGDFVLITGDT 135
           C  + Q+ ++L+ S W        VR I S    S GD LR +  + ++  DF+LI GD 
Sbjct: 91  CWKAAQIKEHLQKSKWCHPTSLNVVRIITSDLYRSLGDVLRDVDAKALVRSDFLLIYGDV 150

Query: 136 ISNMSLTQALQEHKERKKKDSN-AVMTMVVKRSKPSPITHQSRLGTDELFMAIDHNTKQL 195
           +SN+++++AL+EH+ R+K + N +VMTMV K S PS   H +R   D + +A+D  T ++
Sbjct: 151 VSNINISKALEEHRLRRKLEKNVSVMTMVFKESSPS---HPTRCHEDNVVLAVDSTTNRI 210

Query: 196 VYYEDRVDYSKGIIHLDKSLIMDNSS---ISLHNDMQDCYIDICSPEVLSLFTDNFDYQH 255
           ++++     ++G+ H    L +   S   + +  D+ DC+I ICSP+V  LFTDNFDYQ 
Sbjct: 211 LHFQK----TQGLRHFSFPLGLFQGSLDGVEIRYDLLDCHISICSPQVAQLFTDNFDYQ- 270

Query: 256 LRRHFVKGLLL-DDIMGYKIYTHEICSSYAARIDNYRSYDTVSKDIIQRWTYPLVPDVKF 315
            R  FV+GLL+ ++I+G +I+ H     Y AR+ N   Y  V  D+I+RW YPL P+V F
Sbjct: 271 TRDDFVRGLLVNEEILGNQIHLHVTSREYGARVSNLHMYSAVCADVIRRWVYPLTPEVNF 330

Query: 316 FGNST--YKLERQGMYRASEILQARSAQVGPFTVIGDNSKIGDNTKITNSIIGHGCSIGS 375
             +ST  Y   R  +YR  E+     + +    ++G  + +G N  ITNS+IG  C IG 
Sbjct: 331 TDSSTQSYTHSRHNIYRGPEVSLGHGSVLEENVLLGAGTVVGSNCSITNSVIGPNCHIGD 390

Query: 376 NVKIEGSYIWDNVTIEDGCKISNAIVCDGVVIKSGAELEPGVILSFKVVVGDQFTVPSYS 435
           NV ++ +Y+W  V +  G +I  +++CD   +K    L+P  +L+ +VVVG    +P  S
Sbjct: 391 NVVLDQAYLWQGVRVAAGAQIHQSLLCDRAEVKERVILKPHCVLTSQVVVGPDIILPEGS 450

Query: 436 KVSLFEQPTNQDSDEELEYADNSSGIVEFADTTDKSNSALMSNPLEMQGWPASELGKGGL 495
            +SL      +D D+  +++D+S    E                ++++G+  +E+G  G 
Sbjct: 451 VISLHPPDAEEDEDDG-QFSDDSGADQE-------------KEKVKLKGYNPAEVGPEGQ 510

Query: 496 GYVWSI--CEGSVEEEWRHSVAPIPADKLEKVLHKAPDDEVELTQDANVLPPSGELKSDS 555
           GY+W     +   +EE R S+  +  +          ++E E   + +V P   + ++ S
Sbjct: 511 GYLWKAEDVDEKEDEELRQSLWGLMINM---------EEESETESERSVDPEELDSRAGS 570

Query: 556 YVSDGDDNESSRDDSIHFEKEVEATFLRAVHENIEVANVILEVNSLRLSFNKVASDCAGA 615
                       DD   F+ EV  T  R   ENI   N++LE+NSL+ ++N    +    
Sbjct: 571 ---------PQLDDIRVFQNEVLGTLQRGREENISCDNLVLEINSLKYAYNISLKEVMQV 630

Query: 616 IFYSLMNFALDSSRG--SSSELLKNTTSIITKWKKLLKYYLTDLDEEIEVILKFEEICLE 675
           + + ++ F L    G    +        ++  W  + + Y+    + +E +   E+  LE
Sbjct: 631 LSHVVLEFPLQQVDGVLDPNRYCALLLPLLKAWSPVFRNYIKRAADHLEALAAIEDFFLE 690

Query: 676 SAKEFTPHFTKILWQLYDQEIIQEEAILKWDAEKKEADDSDKLFV-KQAEKFIEWLKVAS 730
             +   P   K+L   Y  EI+ EE IL W +++   D   +L   +Q ++FI+WL+ A 
Sbjct: 691 H-ETLVPSLAKVLMAFYQLEILAEETILSWFSQRDITDKGQQLRKNQQLQRFIQWLREAE 716

BLAST of Cucsa.133570 vs. TrEMBL
Match: A0A0A0LJF5_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_2G013230 PE=4 SV=1)

HSP 1 Score: 1458.7 bits (3775), Expect = 0.0e+00
Identity = 731/731 (100.00%), Postives = 731/731 (100.00%), Query Frame = 1

Query: 1   MGAQRKGASRVSDDHEELARVSLQAVLLADSFTTKFRPITLERPKVLLPLVNVPMINYTL 60
           MGAQRKGASRVSDDHEELARVSLQAVLLADSFTTKFRPITLERPKVLLPLVNVPMINYTL
Sbjct: 1   MGAQRKGASRVSDDHEELARVSLQAVLLADSFTTKFRPITLERPKVLLPLVNVPMINYTL 60

Query: 61  SWLESAGVEEVFVFCCANSKQVIDYLENSDWVSLPDFAVRTIESHNSISAGDALRLIYER 120
           SWLESAGVEEVFVFCCANSKQVIDYLENSDWVSLPDFAVRTIESHNSISAGDALRLIYER
Sbjct: 61  SWLESAGVEEVFVFCCANSKQVIDYLENSDWVSLPDFAVRTIESHNSISAGDALRLIYER 120

Query: 121 NVIHGDFVLITGDTISNMSLTQALQEHKERKKKDSNAVMTMVVKRSKPSPITHQSRLGTD 180
           NVIHGDFVLITGDTISNMSLTQALQEHKERKKKDSNAVMTMVVKRSKPSPITHQSRLGTD
Sbjct: 121 NVIHGDFVLITGDTISNMSLTQALQEHKERKKKDSNAVMTMVVKRSKPSPITHQSRLGTD 180

Query: 181 ELFMAIDHNTKQLVYYEDRVDYSKGIIHLDKSLIMDNSSISLHNDMQDCYIDICSPEVLS 240
           ELFMAIDHNTKQLVYYEDRVDYSKGIIHLDKSLIMDNSSISLHNDMQDCYIDICSPEVLS
Sbjct: 181 ELFMAIDHNTKQLVYYEDRVDYSKGIIHLDKSLIMDNSSISLHNDMQDCYIDICSPEVLS 240

Query: 241 LFTDNFDYQHLRRHFVKGLLLDDIMGYKIYTHEICSSYAARIDNYRSYDTVSKDIIQRWT 300
           LFTDNFDYQHLRRHFVKGLLLDDIMGYKIYTHEICSSYAARIDNYRSYDTVSKDIIQRWT
Sbjct: 241 LFTDNFDYQHLRRHFVKGLLLDDIMGYKIYTHEICSSYAARIDNYRSYDTVSKDIIQRWT 300

Query: 301 YPLVPDVKFFGNSTYKLERQGMYRASEILQARSAQVGPFTVIGDNSKIGDNTKITNSIIG 360
           YPLVPDVKFFGNSTYKLERQGMYRASEILQARSAQVGPFTVIGDNSKIGDNTKITNSIIG
Sbjct: 301 YPLVPDVKFFGNSTYKLERQGMYRASEILQARSAQVGPFTVIGDNSKIGDNTKITNSIIG 360

Query: 361 HGCSIGSNVKIEGSYIWDNVTIEDGCKISNAIVCDGVVIKSGAELEPGVILSFKVVVGDQ 420
           HGCSIGSNVKIEGSYIWDNVTIEDGCKISNAIVCDGVVIKSGAELEPGVILSFKVVVGDQ
Sbjct: 361 HGCSIGSNVKIEGSYIWDNVTIEDGCKISNAIVCDGVVIKSGAELEPGVILSFKVVVGDQ 420

Query: 421 FTVPSYSKVSLFEQPTNQDSDEELEYADNSSGIVEFADTTDKSNSALMSNPLEMQGWPAS 480
           FTVPSYSKVSLFEQPTNQDSDEELEYADNSSGIVEFADTTDKSNSALMSNPLEMQGWPAS
Sbjct: 421 FTVPSYSKVSLFEQPTNQDSDEELEYADNSSGIVEFADTTDKSNSALMSNPLEMQGWPAS 480

Query: 481 ELGKGGLGYVWSICEGSVEEEWRHSVAPIPADKLEKVLHKAPDDEVELTQDANVLPPSGE 540
           ELGKGGLGYVWSICEGSVEEEWRHSVAPIPADKLEKVLHKAPDDEVELTQDANVLPPSGE
Sbjct: 481 ELGKGGLGYVWSICEGSVEEEWRHSVAPIPADKLEKVLHKAPDDEVELTQDANVLPPSGE 540

Query: 541 LKSDSYVSDGDDNESSRDDSIHFEKEVEATFLRAVHENIEVANVILEVNSLRLSFNKVAS 600
           LKSDSYVSDGDDNESSRDDSIHFEKEVEATFLRAVHENIEVANVILEVNSLRLSFNKVAS
Sbjct: 541 LKSDSYVSDGDDNESSRDDSIHFEKEVEATFLRAVHENIEVANVILEVNSLRLSFNKVAS 600

Query: 601 DCAGAIFYSLMNFALDSSRGSSSELLKNTTSIITKWKKLLKYYLTDLDEEIEVILKFEEI 660
           DCAGAIFYSLMNFALDSSRGSSSELLKNTTSIITKWKKLLKYYLTDLDEEIEVILKFEEI
Sbjct: 601 DCAGAIFYSLMNFALDSSRGSSSELLKNTTSIITKWKKLLKYYLTDLDEEIEVILKFEEI 660

Query: 661 CLESAKEFTPHFTKILWQLYDQEIIQEEAILKWDAEKKEADDSDKLFVKQAEKFIEWLKV 720
           CLESAKEFTPHFTKILWQLYDQEIIQEEAILKWDAEKKEADDSDKLFVKQAEKFIEWLKV
Sbjct: 661 CLESAKEFTPHFTKILWQLYDQEIIQEEAILKWDAEKKEADDSDKLFVKQAEKFIEWLKV 720

Query: 721 ASEEEEEEEEE 732
           ASEEEEEEEEE
Sbjct: 721 ASEEEEEEEEE 731

BLAST of Cucsa.133570 vs. TrEMBL
Match: M5WLB9_PRUPE (Uncharacterized protein OS=Prunus persica GN=PRUPE_ppa001987mg PE=4 SV=1)

HSP 1 Score: 1147.1 bits (2966), Expect = 0.0e+00
Identity = 567/734 (77.25%), Postives = 645/734 (87.87%), Query Frame = 1

Query: 1   MGAQRKGASRVSDDHEELARVSLQAVLLADSFTTKFRPITLERPKVLLPLVNVPMINYTL 60
           MG Q+K ASR S+D  ELARV LQA+LLADSFTTKFRPITLERPKVLLPLVNVPMINYTL
Sbjct: 1   MGTQKKNASRGSEDPVELARVPLQAILLADSFTTKFRPITLERPKVLLPLVNVPMINYTL 60

Query: 61  SWLESAGVEEVFVFCCANSKQVIDYLENSDWVSLPDFAVRTIESHNSISAGDALRLIYER 120
           +WLESAGVEEVFVFCCA+SKQVIDYLE+S+W S P+F V TIESHNS+SAGDALRLIYER
Sbjct: 61  AWLESAGVEEVFVFCCAHSKQVIDYLESSEWFSQPNFTVTTIESHNSVSAGDALRLIYER 120

Query: 121 NVIHGDFVLITGDTISNMSLTQALQEHKERKKKDSNAVMTMVVKRSKPSPITHQSRLGTD 180
           NVI+GDFVLI+GDT+SNMSLTQ LQEHKER+KKDSNAVMT+V+KRSKPS ITHQSRLGTD
Sbjct: 121 NVINGDFVLISGDTVSNMSLTQVLQEHKERRKKDSNAVMTVVIKRSKPSQITHQSRLGTD 180

Query: 181 ELFMAIDHNTKQLVYYEDRVDYSKGIIHLDKSLIMDNSSISLHNDMQDCYIDICSPEVLS 240
           ELF+AID NTKQL+YYED+ D++KG I+LDK L+ DN SI+LHND QDCYIDICSPEVLS
Sbjct: 181 ELFVAIDPNTKQLLYYEDKADHAKGSIYLDKLLLADNPSITLHNDKQDCYIDICSPEVLS 240

Query: 241 LFTDNFDYQHLRRHFVKGLLLDDIMGYKIYTHEICSSYAARIDNYRSYDTVSKDIIQRWT 300
           LFTDNFDYQHLRRHF+KGLL+DDIMGYKI+THEI SSYAARIDN+RSYDT+SKDIIQRWT
Sbjct: 241 LFTDNFDYQHLRRHFLKGLLVDDIMGYKIFTHEIHSSYAARIDNFRSYDTISKDIIQRWT 300

Query: 301 YPLVPDVKFFGNSTYKLERQGMYRASEILQARSAQVGPFTVIGDNSKIGDNTKITNSIIG 360
           YPLVPDVKFFGNS+ KLERQGMYRASEI Q+RSAQ+GPFTVIG  +KIG+ TKI+NS+IG
Sbjct: 301 YPLVPDVKFFGNSSIKLERQGMYRASEIGQSRSAQIGPFTVIGSGTKIGNTTKISNSVIG 360

Query: 361 HGCSIGSNVKIEGSYIWDNVTIEDGCKISNAIVCDGVVIKSGAELEPGVILSFKVVVGDQ 420
            GCSIGSNV IEGSYIWDNVTIEDGCK+ +AI CDGV++KSGA LEPGV+LSFKVVVG Q
Sbjct: 361 EGCSIGSNVSIEGSYIWDNVTIEDGCKLRHAIACDGVIVKSGAVLEPGVVLSFKVVVGQQ 420

Query: 421 FTVPSYSKVSLFEQPTNQDSDEELEYADNSSGIVEFAD---TTDKSNSALMSNPLEMQGW 480
           F VPSYSKVSL +QP  QDSDEELEYAD+S GI E +    T DK N  + + P E Q W
Sbjct: 421 FIVPSYSKVSLLQQPIKQDSDEELEYADSSGGIAEISSITHTVDKLNGEITNQPFETQCW 480

Query: 481 PASELGKGGLGYVWSICEGSVEEEWRHSVAPIPADKLEKVLHKAPDDEVELTQDANVLPP 540
           P SELG GG GYVWSICEG  +EEWRHSVAPIPADKL + +H A DD++ELTQD   LPP
Sbjct: 481 PTSELGTGGAGYVWSICEGGHDEEWRHSVAPIPADKLSEAIH-AADDDLELTQDGRSLPP 540

Query: 541 SGELKSDSYVSDGDDNESSRDDSIHFEKEVEATFLRAVHENIEVANVILEVNSLRLSFNK 600
           SGELK DS  S+ DD+  SRDDSI+F KEVEATFLRAVHENI V +VILEVNSLRLS+NK
Sbjct: 541 SGELKPDSNDSEYDDDGESRDDSIYFGKEVEATFLRAVHENITVDHVILEVNSLRLSYNK 600

Query: 601 VASDCAGAIFYSLMNFALDSSRGSSSELLKNTTSIITKWKKLLKYYLTDLDEEIEVILKF 660
           VA+DCAG +FYSLM  AL++ + S+SEL++ TT+++ KWKKLLKYYL ++DEEIEVILK 
Sbjct: 601 VAADCAGDLFYSLMKLALETPQDSASELVQATTNVLAKWKKLLKYYLAEIDEEIEVILKL 660

Query: 661 EEICLESAKEFTPHFTKILWQLYDQEIIQEEAILKWDAEKKEADDSDKLFVKQAEKFIEW 720
           EEIC ES KEF P F KIL+QLYD E+IQEEAIL+WD EKK+AD+SDK+FVKQAEKFI+W
Sbjct: 661 EEIC-ESTKEFAPVFDKILYQLYDLEVIQEEAILRWDDEKKDADESDKVFVKQAEKFIQW 720

Query: 721 LKVASEEEEEEEEE 732
           L+ ASEE+EEE+EE
Sbjct: 721 LREASEEDEEEDEE 732

BLAST of Cucsa.133570 vs. TrEMBL
Match: W9RST4_9ROSA (Putative translation initiation factor eIF-2B subunit epsilon OS=Morus notabilis GN=L484_010235 PE=4 SV=1)

HSP 1 Score: 1145.2 bits (2961), Expect = 0.0e+00
Identity = 559/734 (76.16%), Postives = 651/734 (88.69%), Query Frame = 1

Query: 1   MGAQRKGASRVSDDHEELARVSLQAVLLADSFTTKFRPITLERPKVLLPLVNVPMINYTL 60
           MGAQ+KGASRVS+DHE+L RV LQA+LLADSF T FRPITLERPKVLLPLVNVPMINYTL
Sbjct: 1   MGAQKKGASRVSEDHEDLTRVPLQAILLADSFATTFRPITLERPKVLLPLVNVPMINYTL 60

Query: 61  SWLESAGVEEVFVFCCANSKQVIDYLENSDWVSLPDFAVRTIESHNSISAGDALRLIYER 120
           +WLESAGVEEVFVFCCA+SKQVIDYLE S+W S P+F+V+TIESHNS+SAGDALRLIYER
Sbjct: 61  AWLESAGVEEVFVFCCAHSKQVIDYLETSEWFSQPNFSVQTIESHNSVSAGDALRLIYER 120

Query: 121 NVIHGDFVLITGDTISNMSLTQALQEHKERKKKDSNAVMTMVVKRSKPSPITHQSRLGTD 180
           NVIHGDFVLI+GDT+SNMSLT+ALQEHKER+KKD+NAVMTMV+KRSKPS ITHQSRLGTD
Sbjct: 121 NVIHGDFVLISGDTVSNMSLTRALQEHKERRKKDNNAVMTMVIKRSKPSQITHQSRLGTD 180

Query: 181 ELFMAIDHNTKQLVYYEDRVDYSKGIIHLDKSLIMDNSSISLHNDMQDCYIDICSPEVLS 240
           EL+MAID  TKQL+YYED+ D+SKG I+LDK L+ DN+SISLHND QDCYIDICSPEVLS
Sbjct: 181 ELYMAIDPTTKQLLYYEDKADHSKGAIYLDKLLLADNTSISLHNDKQDCYIDICSPEVLS 240

Query: 241 LFTDNFDYQHLRRHFVKGLLLDDIMGYKIYTHEICSSYAARIDNYRSYDTVSKDIIQRWT 300
           LFTDNFDYQHLRRHFVKGLL+DDIMGYKI+THEI SSYAARIDN+RSYDT+SKDIIQRWT
Sbjct: 241 LFTDNFDYQHLRRHFVKGLLVDDIMGYKIFTHEIHSSYAARIDNFRSYDTISKDIIQRWT 300

Query: 301 YPLVPDVKFFGNSTYKLERQGMYRASEILQARSAQVGPFTVIGDNSKIGDNTKITNSIIG 360
           YPLVPDV  F NST KLERQGMYRASEI Q RSA++GPFTVIG+ +KIGDNT I+NS+IG
Sbjct: 301 YPLVPDVMCFENSTTKLERQGMYRASEIGQQRSAKIGPFTVIGNGTKIGDNTSISNSVIG 360

Query: 361 HGCSIGSNVKIEGSYIWDNVTIEDGCKISNAIVCDGVVIKSGAELEPGVILSFKVVVGDQ 420
            GCSIG+NV IEGSYIWDNV IEDGCK+ +AIVCDGV++KSGA LEPGV+LS KVV+G Q
Sbjct: 361 KGCSIGANVSIEGSYIWDNVIIEDGCKLRHAIVCDGVIMKSGAVLEPGVVLSLKVVIGQQ 420

Query: 421 FTVPSYSKVSLFEQPTNQDSDEELEYADNSSGIVE---FADTTDKSNSALMSNPLEMQGW 480
           F VPSYSKVSLF+QPT QDSDEELEYADNSSGIV+     DT DKSN  + S  L+ Q W
Sbjct: 421 FVVPSYSKVSLFQQPTKQDSDEELEYADNSSGIVDIPPMVDTADKSNGEI-SELLDTQRW 480

Query: 481 PASELGKGGLGYVWSICEGSVEEEWRHSVAPIPADKLEKVLHKAPDDEVELTQDANVLPP 540
           P S+LG GG+G++W   EG  +EE RHSVAPIPADKL +++ +A DD++ELTQD ++LPP
Sbjct: 481 PTSQLGSGGIGHIWPFSEGGYDEELRHSVAPIPADKLAELI-QAIDDDLELTQDGSILPP 540

Query: 541 SGELKSDSYVSDGDDNESSRDDSIHFEKEVEATFLRAVHENIEVANVILEVNSLRLSFNK 600
           SGELK DS  S+ DD E SRD+SI+FEKEVEATFLRAVHENI V +V+LEVNSLRLS+N+
Sbjct: 541 SGELKRDSNYSEDDDIEDSRDESIYFEKEVEATFLRAVHENIAVDHVVLEVNSLRLSYNR 600

Query: 601 VASDCAGAIFYSLMNFALDSSRGSSSELLKNTTSIITKWKKLLKYYLTDLDEEIEVILKF 660
           + +DCAGA+FYS+M  AL++   S+ ELL+N T+++TKWKKLLKYYL D+DEEIEVILKF
Sbjct: 601 LTADCAGALFYSMMKLALETPHNSARELLQNATNVVTKWKKLLKYYLADIDEEIEVILKF 660

Query: 661 EEICLESAKEFTPHFTKILWQLYDQEIIQEEAILKWDAEKKEADDSDKLFVKQAEKFIEW 720
           EE+CLESAKEF P FTKI+  LYDQE++QE+AIL+WD EKK+AD+ DK+FVKQAEKFI+W
Sbjct: 661 EEMCLESAKEFAPLFTKIMSHLYDQEVLQEDAILRWDDEKKDADELDKVFVKQAEKFIQW 720

Query: 721 LKVASEEEEEEEEE 732
           L+ A EE++++EEE
Sbjct: 721 LREAPEEDDDKEEE 732

BLAST of Cucsa.133570 vs. TrEMBL
Match: D7U5T4_VITVI (Putative uncharacterized protein OS=Vitis vinifera GN=VIT_15s0045g01250 PE=4 SV=1)

HSP 1 Score: 1082.0 bits (2797), Expect = 0.0e+00
Identity = 531/729 (72.84%), Postives = 632/729 (86.69%), Query Frame = 1

Query: 4   QRKGASRVSDDHEELARVSLQAVLLADSFTTKFRPITLERPKVLLPLVNVPMINYTLSWL 63
           QRKG++RVS+D EEL RV LQA+LLADSF  KFRPITLERPKVLLPLVNVPMI+YTL+WL
Sbjct: 3   QRKGSTRVSEDAEELVRVPLQAILLADSFAQKFRPITLERPKVLLPLVNVPMIDYTLAWL 62

Query: 64  ESAGVEEVFVFCCANSKQVIDYLENSDWVSLPDFAVRTIESHNSISAGDALRLIYERNVI 123
           ESAG+EEVFVFCCA+SKQVI+YLENS WVSLP F V TIESHNS+SAGDALRLIYER+VI
Sbjct: 63  ESAGIEEVFVFCCAHSKQVINYLENSHWVSLPHFEVTTIESHNSVSAGDALRLIYERHVI 122

Query: 124 HGDFVLITGDTISNMSLTQALQEHKERKKKDSNAVMTMVVKRSKPSPITHQSRLGTDELF 183
           HGDFVLITGDT+SNMSLTQALQEHK+R+KKD+NAVMTMV+KRSKPSPITHQSRLGTDELF
Sbjct: 123 HGDFVLITGDTVSNMSLTQALQEHKDRRKKDNNAVMTMVIKRSKPSPITHQSRLGTDELF 182

Query: 184 MAIDHNTKQLVYYEDRVDYSKGIIHLDKSLIMDNSSISLHNDMQDCYIDICSPEVLSLFT 243
           MAID +TKQL+YYED+ D+ KG + L+K L+ DN SISLHND QDCYIDICSPEVLS+FT
Sbjct: 183 MAIDPHTKQLLYYEDKADHLKGTLSLEKMLLADNPSISLHNDKQDCYIDICSPEVLSIFT 242

Query: 244 DNFDYQHLRRHFVKGLLLDDIMGYKIYTHEICSSYAARIDNYRSYDTVSKDIIQRWTYPL 303
           DNFDYQHLRRHF+KGLL+DDIMGYKI+THEI S+YAARIDN+RSYDT+SKDIIQRWTYPL
Sbjct: 243 DNFDYQHLRRHFIKGLLVDDIMGYKIFTHEIHSNYAARIDNFRSYDTISKDIIQRWTYPL 302

Query: 304 VPDVKFFGNSTYKLERQGMYRASEILQARSAQVGPFTVIGDNSKIGDNTKITNSIIGHGC 363
           VPDV+F GN   KLERQGMYRA +I Q+RSAQ+GPF++IG+ + IGDNTKI+NS+IG  C
Sbjct: 303 VPDVQFLGNCAAKLERQGMYRALDIGQSRSAQIGPFSIIGNGTNIGDNTKISNSVIGERC 362

Query: 364 SIGSNVKIEGSYIWDNVTIEDGCKISNAIVCDGVVIKSGAELEPGVILSFKVVVGDQFTV 423
           +IGSNV IEGSYIWDNVTIEDGC + +AIVC+ V++KSGA LEPGVILSFKVV+G +F V
Sbjct: 363 TIGSNVSIEGSYIWDNVTIEDGCVLKHAIVCNDVIMKSGAVLEPGVILSFKVVIGQEFVV 422

Query: 424 PSYSKVSLFEQPTNQDSDEELEYADNSSGIVE---FADTTDKSNSALMSNPLEMQGWPAS 483
           P+YSKVSL +QPT QDSDEELEYAD++SG+ E      T DK N  L     E + W  S
Sbjct: 423 PAYSKVSLLQQPTKQDSDEELEYADHNSGVEEIPSITSTVDKLNGVLTCKLPETEAWSTS 482

Query: 484 ELGKGGLGYVWSICEGSVEEEWRHSVAPIPADKLEKVLHKAPDDEVEL-TQDANVLPPSG 543
           E+G GG+GY+WSICEG  EEEWRHSVAP PADKL +V+ +A DD++EL TQ +NVL PSG
Sbjct: 483 EVGSGGVGYIWSICEGGHEEEWRHSVAPTPADKLTEVM-QATDDDLELTTQGSNVLLPSG 542

Query: 544 EL--KSDSYVSDGDDNESSRDDSIHFEKEVEATFLRAVHENIEVANVILEVNSLRLSFNK 603
           EL   S+S +S+ DDNE SRDDS++FEKEVEATFLRAVHEN++  +VILEVNSLRLS+N 
Sbjct: 543 ELVPDSNSNISEDDDNEDSRDDSVYFEKEVEATFLRAVHENVKEDHVILEVNSLRLSYNM 602

Query: 604 VASDCAGAIFYSLMNFALDSSRGSSSELLKNTTSIITKWKKLLKYYLTDLDEEIEVILKF 663
            ++DCAGA+FYS+M  AL++   S+SELLKN  S+IT+W+KL+K YL  +DEEIEVILKF
Sbjct: 603 TSADCAGALFYSVMKLALETPCNSNSELLKNVASVITRWQKLIKSYLQSMDEEIEVILKF 662

Query: 664 EEICLESAKEFTPHFTKILWQLYDQEIIQEEAILKWDAEKKEADDSDKLFVKQAEKFIEW 723
           EE+CLESAKEF+  F +IL  LY+++I+QE+AILKW +EK+ A++SD++FVKQ+EKFI+W
Sbjct: 663 EEMCLESAKEFSALFAQILHILYEKDILQEDAILKWASEKEGAEESDRVFVKQSEKFIQW 722

Query: 724 LKVASEEEE 727
           L  ASEEE+
Sbjct: 723 LNEASEEED 730

BLAST of Cucsa.133570 vs. TrEMBL
Match: V4T507_9ROSI (Uncharacterized protein OS=Citrus clementina GN=CICLE_v10019027mg PE=4 SV=1)

HSP 1 Score: 1073.2 bits (2774), Expect = 1.6e-310
Identity = 531/735 (72.24%), Postives = 626/735 (85.17%), Query Frame = 1

Query: 1   MGAQRK------GASRVSDDHEELARVSLQAVLLADSFTTKFRPITLERPKVLLPLVNVP 60
           MGAQ+K       A+RVS+D ++LAR  LQA+LLADSFTTKFRPITLERPKVLLPLVNVP
Sbjct: 1   MGAQKKRAAAATAAARVSEDPDDLARQPLQAILLADSFTTKFRPITLERPKVLLPLVNVP 60

Query: 61  MINYTLSWLESAGVEEVFVFCCANSKQVIDYLENSDWVSLPDFAVRTIESHNSISAGDAL 120
           MINYTL+WLE+AGV EVFVFCCA+SKQVIDYLENS+W S P+F V+TIESHN ISAGDAL
Sbjct: 61  MINYTLAWLEAAGVAEVFVFCCAHSKQVIDYLENSEWFSQPNFTVKTIESHNIISAGDAL 120

Query: 121 RLIYERNVIHGDFVLITGDTISNMSLTQALQEHKERKKKDSNAVMTMVVKRSKPSPITHQ 180
           RLIYE+NVIHGDFVLI+GDT+SNM LTQALQEHKERKKKD+NAVMTM++K+SKPSPITHQ
Sbjct: 121 RLIYEQNVIHGDFVLISGDTVSNMLLTQALQEHKERKKKDNNAVMTMIIKKSKPSPITHQ 180

Query: 181 SRLGTDELFMAIDHNTKQLVYYEDRVDYSKGIIHLDKSLIMDNSSISLHNDMQDCYIDIC 240
           SRLGTDELFMAID NTKQL+YYED+ D+SKG I LDK L+ +N SISLHND QDCYIDIC
Sbjct: 181 SRLGTDELFMAIDPNTKQLLYYEDKADHSKGTICLDKMLLAENPSISLHNDKQDCYIDIC 240

Query: 241 SPEVLSLFTDNFDYQHLRRHFVKGLLLDDIMGYKIYTHEICSSYAARIDNYRSYDTVSKD 300
           SPEVLSLFTDNFDYQHLRRHFVKGLLLDDIMGYKI+THEI SSYAARIDNYRSYD VSKD
Sbjct: 241 SPEVLSLFTDNFDYQHLRRHFVKGLLLDDIMGYKIFTHEIHSSYAARIDNYRSYDIVSKD 300

Query: 301 IIQRWTYPLVPDVKFFGNSTYKLERQGMYRASEILQARSAQVGPFTVIGDNSKIGDNTKI 360
           IIQRWTYP VPDVKF GN   KLER+GMYRASEI Q+RSAQVG FTVIG  +KIG N+KI
Sbjct: 301 IIQRWTYPYVPDVKFCGNRATKLERRGMYRASEIEQSRSAQVGSFTVIGCGTKIGSNSKI 360

Query: 361 TNSIIGHGCSIGSNVKIEGSYIWDNVTIEDGCKISNAIVCDGVVIKSGAELEPGVILSFK 420
           ++S+IG GC+IGSNV IEGSY+WDNV IEDGC++ + IVCDGV++K+GA L+PGV+LSFK
Sbjct: 361 SDSVIGEGCTIGSNVLIEGSYLWDNVIIEDGCELRHVIVCDGVIMKAGAVLKPGVVLSFK 420

Query: 421 VVVGDQFTVPSYSKVSLFEQPTNQDSDEELEYADNSSGIVEFAD---TTDKSNSALMSNP 480
           VV+G++F VP+YSKVSL +QPT  DSDEELEYADNSSG VEF+    T D+SN  + S  
Sbjct: 421 VVIGERFVVPAYSKVSLLQQPTVHDSDEELEYADNSSGTVEFSSIQGTADQSNGEMTSES 480

Query: 481 LEMQGWPASELGKGGLGYVWSICEGSVEEEWRHSVAPIPADKLEKVLHKAPDDEVELTQD 540
            E       +LG GG+GY+WSICEG  EEEWRHSVAPIP DKL ++     DD+  +TQD
Sbjct: 481 SEAH---KPKLGTGGVGYIWSICEGGQEEEWRHSVAPIPEDKLAELSEAMDDDQELVTQD 540

Query: 541 ANVLPPSGELKSDSYVSDGDDNESSRDDSIHFEKEVEATFLRAVHENIEVANVILEVNSL 600
              L  SGEL SDS  S+GDDNE S+DDS++FEKEVEATFLRAV EN++V +VILEVNSL
Sbjct: 541 RTALSTSGELISDSNASEGDDNEDSKDDSVYFEKEVEATFLRAVEENVKVDHVILEVNSL 600

Query: 601 RLSFNKVASDCAGAIFYSLMNFALDSSRGSSSELLKNTTSIITKWKKLLKYYLTDLDEEI 660
           RLS+N  ++DCAGA+FYS+M  A+ +   S+SEL +NT +IIT W+KLLK YL ++DEEI
Sbjct: 601 RLSYNMTSADCAGAVFYSMMKLAVKTPHSSASELQQNTANIITTWQKLLKSYLLEIDEEI 660

Query: 661 EVILKFEEICLESAKEFTPHFTKILWQLYDQEIIQEEAILKWDAEKKEADDSDKLFVKQA 720
           E+ILKFEE+CLESAKEF+P F +IL  LYD++I+QE+AIL+W  EK+ AD+SDK+FV+Q+
Sbjct: 661 EIILKFEEMCLESAKEFSPLFARILHILYDKDILQEDAILRWADEKEGADESDKVFVRQS 720

Query: 721 EKFIEWLKVASEEEE 727
           EKF++WL+ ASEEE+
Sbjct: 721 EKFVQWLREASEEED 732

BLAST of Cucsa.133570 vs. TAIR10
Match: AT2G34970.1 (AT2G34970.1 Trimeric LpxA-like enzyme)

HSP 1 Score: 936.8 bits (2420), Expect = 8.1e-273
Identity = 470/738 (63.69%), Postives = 583/738 (79.00%), Query Frame = 1

Query: 1   MGAQRKG--ASRVSDDHEELARVSLQAVLLADSFTTKFRPITLERPKVLLPLVNVPMINY 60
           MGAQ+KG  A+RVS+D E  +R  LQA+LLADSF TKFRP+TLERPKVLLP+VNVPMI+Y
Sbjct: 1   MGAQKKGGAAARVSEDAEVQSRHRLQAILLADSFATKFRPVTLERPKVLLPIVNVPMIDY 60

Query: 61  TLSWLESAGVEEVFVFCCANSKQVIDYLENSDWVSLPDFAVRTIESHNSISAGDALRLIY 120
           TL+WLESAG+EEVFVFCCA+S QVI+YLE S+W S P+  VRTIESH SISAGDALR +Y
Sbjct: 61  TLAWLESAGIEEVFVFCCAHSMQVIEYLEKSEWYSHPNLLVRTIESHKSISAGDALRYMY 120

Query: 121 ERNV----IHGDFVLITGDTISNMSLTQALQEHKERKKKDSNAVMTMVVKRSKPSPITHQ 180
           E+      I GDFVL++GDT+SNM L   +QEH+ERKKKD  A+MTMV+K+SK SP+THQ
Sbjct: 121 EQQTETSQIQGDFVLVSGDTVSNMPLADLIQEHRERKKKDEKAIMTMVIKQSKSSPLTHQ 180

Query: 181 SRLGTDELFMAIDHNTKQLVYYE-DRVDYSKGIIHLDKSLIMDNSSISLHNDMQDCYIDI 240
           SRLGTD+LF+A+D  TKQL++YE D++D+  G + L+KSL+  N S+ + NDMQDCYIDI
Sbjct: 181 SRLGTDQLFIAVDPLTKQLLHYEEDKIDHPSGSVCLEKSLLDTNPSVLVCNDMQDCYIDI 240

Query: 241 CSPEVLSLFTDNFDYQHLRRHFVKGLLLDDIMGYKIYTHEICSSYAARIDNYRSYDTVSK 300
           CSPEVLSLF DNFDYQHLRRHFVKG+L+DDIMGYKI+THEI SSYA RIDN+RSYDTVSK
Sbjct: 241 CSPEVLSLFEDNFDYQHLRRHFVKGVLVDDIMGYKIFTHEIHSSYAGRIDNFRSYDTVSK 300

Query: 301 DIIQRWTYPLVPDVKFFGNSTYKLERQGMYRASEILQARSAQVGPFTVIGDNSKIGDNTK 360
           DIIQRWTYP VPD+ F GN   KL RQG+Y+AS+++Q+RSA VG  TVIG  +KIG   K
Sbjct: 301 DIIQRWTYPYVPDINFSGNRPLKLGRQGIYKASDVVQSRSADVGASTVIGYGTKIGHGDK 360

Query: 361 ITNSIIGHGCSIGSNVKIEGSYIWDNVTIEDGCKISNAIVCDGVVIKSGAELEPGVILSF 420
           I NS+IG+GCSIGSNV IEGSYIW+NVTIEDGC+I NAIVCDGV I++GA L+PGV+LSF
Sbjct: 361 IMNSVIGNGCSIGSNVVIEGSYIWNNVTIEDGCEIRNAIVCDGVKIRAGAVLQPGVVLSF 420

Query: 421 KVVVGDQFTVPSYSKVSLFEQPTNQDSDEELEYADNSSGIVEFADTTDKSNSALMSNPLE 480
            VVVG  F VP+YSKVSL +QPT +DSDEELEYAD+SSG  +           L    L+
Sbjct: 421 NVVVGRDFVVPAYSKVSLLQQPTTEDSDEELEYADSSSGTADH----------LSGLNLQ 480

Query: 481 MQGWPASELGKGGLGYVWSICEGSVEEEWRHSVAPIPADKLEKVLHKAPDDEVELTQDAN 540
           M+   ASELG  G GY+W +CEG+ +EEW+HSVAPIP DKL ++     DD+   T D +
Sbjct: 481 MES-KASELGPDGAGYIWEVCEGAHDEEWKHSVAPIPKDKLSEITQAIDDDD---TDDES 540

Query: 541 VLPPSGELKSDSYVSDGDDNESSRDDSIHFEKEVEATFLRAVHENIEVANVILEVNSLRL 600
           V+P SGELKSD+   + D N+ + DD  +FEKEVE T LRAV ENI+V  V +E+N LRL
Sbjct: 541 VVPTSGELKSDADSINTDVNDPN-DDYYYFEKEVEGTVLRAVEENIKVDLVTMEINGLRL 600

Query: 601 SFNKVASDCAGAIFYSLMNFALDSSRGSSSELLKNTTSIITKWKKLLKYYLTDLDEEIEV 660
           SFN  ++DCAGA F+S++  ALD+   S SEL KN  SIITKWK LL +Y   +DE+IEV
Sbjct: 601 SFNMESADCAGATFFSMIKLALDTPHNSGSELYKNAASIITKWKDLLGFYAKKIDEQIEV 660

Query: 661 ILKFEEICLESAKEFTPHFTKILWQLYDQEIIQEEAILKWDAEKKEADDSDKLFVKQAEK 720
           I+KFEE+C ES KE  P FT+IL  LYD++++QE+AIL+W+ EK  AD++DK+++KQ + 
Sbjct: 661 IMKFEEMCQESHKELGPLFTQILHLLYDKDVLQEDAILRWEEEKAGADEADKVYLKQCDT 720

Query: 721 FIEWLKVASEEEEEEEEE 732
           FI+WLK ASEEE+E++E+
Sbjct: 721 FIQWLKEASEEEDEDDED 723

BLAST of Cucsa.133570 vs. TAIR10
Match: AT4G18300.1 (AT4G18300.1 Trimeric LpxA-like enzyme)

HSP 1 Score: 824.7 bits (2129), Expect = 4.5e-239
Identity = 434/738 (58.81%), Postives = 553/738 (74.93%), Query Frame = 1

Query: 1   MGAQRK-GASRVSDDHEELARVSLQAVLLADSFTTKFRPITLERPKVLLPLVNVPMINYT 60
           MGAQ+K  A++V +D E+L+R  LQA+LLADSFTT+FR ITLERPKVLLP+VN+PMI+YT
Sbjct: 1   MGAQKKPAAAKVREDSEDLSRQRLQAILLADSFTTQFRSITLERPKVLLPIVNIPMIDYT 60

Query: 61  LSWLESAGVEEVFVFCCANSKQVIDYLENSDWVSLPD--FAVRTIESHNSISAGDALRLI 120
           L+WLESAG+EEVFVFCCA+S Q+IDYL+ S+W + P+    VRTI SHNS S GDALR I
Sbjct: 61  LAWLESAGIEEVFVFCCAHSSQIIDYLKKSEWYTRPNPNLLVRTIVSHNSTSVGDALRYI 120

Query: 121 YERNV----IHGDFVLITGDTISNMSLTQALQEHKERKKKDSNAVMTMVVKRSKPSPITH 180
           YE+      I GDFVL++GDT+SNM L   +Q+H++RKKKD  A+MTMV K+        
Sbjct: 121 YEQQTETSQIQGDFVLVSGDTVSNMPLADLIQQHRDRKKKDEKAIMTMVFKK-------- 180

Query: 181 QSRLG--TDELFMAIDHNTKQLVYYEDRVDYSKGIIHLDKSLIMDNSSISLHNDMQDCYI 240
           QSRLG  +D+LF+A+D  TKQL++YE+  +   G   LDKSL+    S  L +DMQDCYI
Sbjct: 181 QSRLGIGSDQLFVAVDPLTKQLLHYEE-CNSRGGDFCLDKSLL---DSTVLCSDMQDCYI 240

Query: 241 DICSPEVLSLFTDNFDYQHLRRHFVKGLLLDDIMGYKIYTHEICSS-YAARIDNYRSYDT 300
           DICSPEVLSLF DNFDYQH+RRHFV GLL+DDIMGYKI+THEI SS YAARIDN RSYDT
Sbjct: 241 DICSPEVLSLFEDNFDYQHMRRHFVNGLLVDDIMGYKIFTHEIQSSCYAARIDNLRSYDT 300

Query: 301 VSKDIIQRWTYPLVPDVKFFGNSTYKLERQGMYRASEILQARSAQVGPFTVIGDNSKIGD 360
           VSKDIIQRWTYP VP++ F GN   KL R+G+YRAS+ +Q+ +A VG  TVIG  +KIG+
Sbjct: 301 VSKDIIQRWTYPYVPNIDFSGNRPVKLGRRGIYRASDAVQSHTADVGASTVIGYGTKIGN 360

Query: 361 NTKITNSIIGHGCSIGSNVKIEGSYIWDNVTIEDGCKISNAIVCDGVVIKSGAELEPGVI 420
             KI NS+IG+GC IGSNV I+GSYIW+NVTIEDGC+I NAIVCDGV +++GA L+PGV+
Sbjct: 361 GGKIYNSVIGNGCCIGSNVVIQGSYIWNNVTIEDGCEIRNAIVCDGVKVRAGAVLQPGVV 420

Query: 421 LSFKVVVGDQFTVPSYSKVSLFEQPTNQDSDEELEYADNSSGIVEFADTTDKSNSALMSN 480
           LSF VVVG  F VP+YSKVSL +QPT +DSDEELEYAD+SSG      T D S+     N
Sbjct: 421 LSFSVVVGRDFVVPAYSKVSLLQQPTTEDSDEELEYADSSSG------TADHSSGL---N 480

Query: 481 PLEMQGWPASELGKGGLGYVWSICEGSVEEEWRHSVAPIPADKLEKVLHKAPDDEVELTQ 540
            L+++   ASELG  G GY+W IC+ + +EEW+HSV PIP DKL  +     DD++E   
Sbjct: 481 NLQIES-KASELGPDGAGYIWEICDSAHDEEWKHSVVPIPMDKLADITGAMDDDDIE--- 540

Query: 541 DANVLPPSGELKSDSYVSDGDDNESSRDDSIHFEKEVEATFLRAVHENIEVANVILEVNS 600
           D  V+PPSGELKSD+ + + D N+S  D    FE+EVE  FL AV ++ +   VI  +NS
Sbjct: 541 DDIVVPPSGELKSDTDIINTDANDSYGD----FEREVEEEFLSAVEKDNKADIVIPLINS 600

Query: 601 LRLSFNKVASDCAGAIFYSLMNFALDSSRGSSSELLKNTTSIITKWKKLLKYYLTDLDEE 660
            RL++N  ++DCAGA+FYS+M  A+ S   S++EL +N   +ITKWK +L +YL   D +
Sbjct: 601 HRLAYNMDSADCAGAVFYSMMRLAVKSPHNSATELYRNAMGVITKWKGVLGFYLKQTDAQ 660

Query: 661 IEVILKFEEICLESAKEFTPHFTKILWQLYDQEIIQEEAILKWDAEKKEADDSDKLFVKQ 720
           IEVI+KFEE+C ES +E +P F K+L  LYD++++QE+AIL+W  EK  ADD DK+++ +
Sbjct: 661 IEVIMKFEEMCQES-EELSPLFAKLLPFLYDKDVVQEDAILRWGEEKAGADDCDKVYLNK 708

Query: 721 AEKFIEWLKVASEEEEEE 729
            E FI+WLK ASEEEEE+
Sbjct: 721 CESFIKWLKEASEEEEED 708

BLAST of Cucsa.133570 vs. TAIR10
Match: AT3G02270.1 (AT3G02270.1 Trimeric LpxA-like enzyme)

HSP 1 Score: 728.0 bits (1878), Expect = 5.7e-210
Identity = 396/735 (53.88%), Postives = 507/735 (68.98%), Query Frame = 1

Query: 1   MGAQRKGASRVSDDHEE--LARVSLQAVLLADSFTTKFRPITLERPKVLLPLVNVPMINY 60
           M +++K A+++S+D EE    R  LQA+LLADSF TK  P+TLERP VLLPLVN+PMI+Y
Sbjct: 1   MASRKKRAAKISEDSEEEQSRRQRLQAILLADSFATKLLPLTLERPNVLLPLVNIPMIDY 60

Query: 61  TLSWLESAGVEEVFVFCCANSKQVIDYLENSDWVSLPDFAVRTIES-HNSISAGDALRLI 120
           TL+WLESAG+EEVFVFC   S QVIDYL NSDW S  DF V+TIES  NS SAGDALR I
Sbjct: 61  TLAWLESAGIEEVFVFC---SMQVIDYLNNSDWYSHKDFTVKTIESPQNSTSAGDALRYI 120

Query: 121 YERNV----IHGDFVLITGDTISNMSLTQALQEHKERKKKDSNAVMTMVVKRSKPSPITH 180
           YE+ +    I GDFVL+ G  +SNM LTQ +QEH++RKKKD  A+MTMV++         
Sbjct: 121 YEQQIETSQIQGDFVLVNGCIVSNMPLTQLIQEHRDRKKKDEKAIMTMVIR--------- 180

Query: 181 QSRLGTDELFMAIDHNTKQLVYYEDRVDYSKGIIHLDKSLIMDNSSISLHNDMQDCYIDI 240
           QS +   +LF+A++  TKQL+YY++        I  DKSL+  N S+ L +DMQDCYIDI
Sbjct: 181 QSLITDHQLFIAVNPLTKQLLYYDE------DNICFDKSLLDRNPSVLLCSDMQDCYIDI 240

Query: 241 CSPEVLSLFTDNFDYQHLRRHFVKGLLLDDIMGYKIYTHEICSSYAARIDNYRSYDTVSK 300
           CS EVLSLF DNFDYQH+R  FV+G+L DDI+GYKI+THEI S YA+RI+N+RSYD VSK
Sbjct: 241 CSLEVLSLFVDNFDYQHMRCDFVEGVLADDIIGYKIFTHEISSCYASRIENFRSYDMVSK 300

Query: 301 DIIQRWTYPLVPDVKFFGNSTYKLERQGMYRASEILQARSAQVGPFTVIGDNSKIGDNTK 360
           DIIQR T+P VPD+KF GN T KLERQG+Y+AS+  Q  SA VG   VIG  + IG  TK
Sbjct: 301 DIIQRRTFPYVPDMKFSGNRTLKLERQGIYKASDATQLPSAHVGASYVIGHATNIGSGTK 360

Query: 361 ITNSIIGHGCSIGSNVKIEGSYIWDNVTIEDGCKISNAIVCDGVVIKSGAELEPGVILSF 420
           I NS+IG+GCSIGSNV I+GSYIW+NVT+EDGC+I NAIVCD V + +GA ++PGV+LSF
Sbjct: 361 ILNSVIGNGCSIGSNVVIQGSYIWNNVTVEDGCEIRNAIVCDEVKVCAGAIVKPGVVLSF 420

Query: 421 KVVVGDQFTVPSYSKVSLFEQPTNQDSDEELEYADNSSGIVEFADTTDKSNSALMSNPLE 480
           KVVVG  F VP+YS+VSL  QP  +DSDEE                     + L    L+
Sbjct: 421 KVVVGRDFVVPAYSQVSLLRQPMEEDSDEE---------------------NLLSGVDLQ 480

Query: 481 MQGWPASELGKGGLGYVWSICEGSVEEEWRHSVAPIPADKLEKVLHKAPDDEVELTQDAN 540
           M+    S+LG  G GY+W     + E+EW+HSV PIP DKL +++    DD+   T D +
Sbjct: 481 ME----SKLGLDGAGYIW---RQACEDEWKHSVPPIPKDKLAEIIKAIDDDD---TDDES 540

Query: 541 VLPPSGELKSDSYVSDGDDNESSRDDSIHFEKEVEATFLRAVHENIEVANVILEVNSLRL 600
           V+  S           GD N S  +D   FE+EV+ TFLRAV ENI     +LE+NSLRL
Sbjct: 541 VVTTS-----------GDANTSINNDLFDFEREVDGTFLRAVEENIVADLAVLEINSLRL 600

Query: 601 SFNKVASDCAGAIFYSLMNFALDSSRGSSSELLKNTTSIITKWKKLLKYYLTDLDEEIEV 660
           S+N  ++ CAGAIFYS+M  A+ +   S ++L +N +SIIT+WK LL +Y+   DE+IEV
Sbjct: 601 SYNMESAHCAGAIFYSMMKLAVSTPHSSINDLYRNASSIITRWKGLLGFYVKKSDEQIEV 660

Query: 661 ILKFEEICLESAKEFTPHFTKILWQLYDQE--IIQEEAILKWDAEKKEADDSDKLFVKQA 720
           I + EE+C ESA E    F  IL  +Y++E  ++QE AIL+W  EK  AD+SDK+++KQ 
Sbjct: 661 ISRLEEMCEESAHELGTLFAHILRYMYEEENDLLQEVAILRWSDEKAGADESDKVYLKQC 675

Query: 721 EKFIEWLKVASEEEE 727
           E FI WLK  S++E+
Sbjct: 721 EPFITWLKETSDDED 675

BLAST of Cucsa.133570 vs. TAIR10
Match: AT5G19485.1 (AT5G19485.1 transferases;nucleotidyltransferases)

HSP 1 Score: 73.9 bits (180), Expect = 4.5e-13
Identity = 55/208 (26.44%), Postives = 98/208 (47.12%), Query Frame = 1

Query: 210 DKSLIMDNSSISLHNDMQDCYIDICSPEVLSLFTDNFDYQHLRRHFVKGLLLDDIMGYKI 269
           D+S + +N + +  N+MQ+  +      VLS    N       + +  GL      G + 
Sbjct: 253 DQSNVEENGNGNGKNNMQNNEV------VLSQILSNASMPSFHQVYESGL------GTR- 312

Query: 270 YTHEIC------SSYAARIDNYRSYDTVSKDIIQRWTYPLVPDVKFFGNSTYKLERQGMY 329
            TH+ C      S Y  R+++ +++  V++D+I    +            ++      ++
Sbjct: 313 KTHKCCVYIADESKYFVRLNSIQAFMDVNRDVIGESNH--------LSGYSFSAHHNIVH 372

Query: 330 RASEILQARSAQVGPFTVIGDNSKIGDNTKITNSIIGHGCSIGSNVKIEGSYIWDNVTIE 389
            ++E+       VGP  ++G+ S++GD   +  S+IG  C IGSNVKI  S + D+ TI 
Sbjct: 373 PSAEL--GSKTTVGPHCMLGEGSQVGDKCSVKRSVIGRHCRIGSNVKIVNSVVMDHATIG 431

Query: 390 DGCKISNAIVCDGVVIKSGAELEPGVIL 412
           DGC I  +++C      S A+L+  V L
Sbjct: 433 DGCSIQGSVIC------SNAQLQERVTL 431

BLAST of Cucsa.133570 vs. TAIR10
Match: AT2G39770.1 (AT2G39770.1 Glucose-1-phosphate adenylyltransferase family protein)

HSP 1 Score: 55.1 bits (131), Expect = 2.2e-07
Identity = 40/165 (24.24%), Postives = 82/165 (49.70%), Query Frame = 1

Query: 23  LQAVLLADSFTTKFRPITLERPKVLLPLVNVPMINYTLSWLESAGVEEVFVFCCANSKQV 82
           ++A++L   F T+ RP+TL  PK L+   N PMI + +  L++ GV+EV +      + +
Sbjct: 1   MKALILVGGFGTRLRPLTLSFPKPLVDFANKPMILHQIEALKAVGVDEVVLAINYQPEVM 60

Query: 83  IDYLENSDWVSLPDFAVRTIESHNSISAGDALRLIYERNVIHGD---FVLITGDTISNMS 142
           +++L+  D+ +  +  +   +    +     L L  ++ ++ G    F ++  D IS   
Sbjct: 61  LNFLK--DFETKLEIKITCSQETEPLGTAGPLALARDK-LLDGSGEPFFVLNSDVISEYP 120

Query: 143 LTQALQEHKERKKKDSNAVMTMVVKRSKPSPITHQSRLGTDELFM 185
           L + L+ HK     +++ ++T V + SK   +  +   G  E F+
Sbjct: 121 LKEMLEFHKSH-GGEASIMVTKVDEPSKYGVVVMEESTGRVEKFV 161

BLAST of Cucsa.133570 vs. NCBI nr
Match: gi|778666635|ref|XP_011648780.1| (PREDICTED: translation initiation factor eIF-2B subunit epsilon isoform X1 [Cucumis sativus])

HSP 1 Score: 1458.7 bits (3775), Expect = 0.0e+00
Identity = 731/731 (100.00%), Postives = 731/731 (100.00%), Query Frame = 1

Query: 1   MGAQRKGASRVSDDHEELARVSLQAVLLADSFTTKFRPITLERPKVLLPLVNVPMINYTL 60
           MGAQRKGASRVSDDHEELARVSLQAVLLADSFTTKFRPITLERPKVLLPLVNVPMINYTL
Sbjct: 1   MGAQRKGASRVSDDHEELARVSLQAVLLADSFTTKFRPITLERPKVLLPLVNVPMINYTL 60

Query: 61  SWLESAGVEEVFVFCCANSKQVIDYLENSDWVSLPDFAVRTIESHNSISAGDALRLIYER 120
           SWLESAGVEEVFVFCCANSKQVIDYLENSDWVSLPDFAVRTIESHNSISAGDALRLIYER
Sbjct: 61  SWLESAGVEEVFVFCCANSKQVIDYLENSDWVSLPDFAVRTIESHNSISAGDALRLIYER 120

Query: 121 NVIHGDFVLITGDTISNMSLTQALQEHKERKKKDSNAVMTMVVKRSKPSPITHQSRLGTD 180
           NVIHGDFVLITGDTISNMSLTQALQEHKERKKKDSNAVMTMVVKRSKPSPITHQSRLGTD
Sbjct: 121 NVIHGDFVLITGDTISNMSLTQALQEHKERKKKDSNAVMTMVVKRSKPSPITHQSRLGTD 180

Query: 181 ELFMAIDHNTKQLVYYEDRVDYSKGIIHLDKSLIMDNSSISLHNDMQDCYIDICSPEVLS 240
           ELFMAIDHNTKQLVYYEDRVDYSKGIIHLDKSLIMDNSSISLHNDMQDCYIDICSPEVLS
Sbjct: 181 ELFMAIDHNTKQLVYYEDRVDYSKGIIHLDKSLIMDNSSISLHNDMQDCYIDICSPEVLS 240

Query: 241 LFTDNFDYQHLRRHFVKGLLLDDIMGYKIYTHEICSSYAARIDNYRSYDTVSKDIIQRWT 300
           LFTDNFDYQHLRRHFVKGLLLDDIMGYKIYTHEICSSYAARIDNYRSYDTVSKDIIQRWT
Sbjct: 241 LFTDNFDYQHLRRHFVKGLLLDDIMGYKIYTHEICSSYAARIDNYRSYDTVSKDIIQRWT 300

Query: 301 YPLVPDVKFFGNSTYKLERQGMYRASEILQARSAQVGPFTVIGDNSKIGDNTKITNSIIG 360
           YPLVPDVKFFGNSTYKLERQGMYRASEILQARSAQVGPFTVIGDNSKIGDNTKITNSIIG
Sbjct: 301 YPLVPDVKFFGNSTYKLERQGMYRASEILQARSAQVGPFTVIGDNSKIGDNTKITNSIIG 360

Query: 361 HGCSIGSNVKIEGSYIWDNVTIEDGCKISNAIVCDGVVIKSGAELEPGVILSFKVVVGDQ 420
           HGCSIGSNVKIEGSYIWDNVTIEDGCKISNAIVCDGVVIKSGAELEPGVILSFKVVVGDQ
Sbjct: 361 HGCSIGSNVKIEGSYIWDNVTIEDGCKISNAIVCDGVVIKSGAELEPGVILSFKVVVGDQ 420

Query: 421 FTVPSYSKVSLFEQPTNQDSDEELEYADNSSGIVEFADTTDKSNSALMSNPLEMQGWPAS 480
           FTVPSYSKVSLFEQPTNQDSDEELEYADNSSGIVEFADTTDKSNSALMSNPLEMQGWPAS
Sbjct: 421 FTVPSYSKVSLFEQPTNQDSDEELEYADNSSGIVEFADTTDKSNSALMSNPLEMQGWPAS 480

Query: 481 ELGKGGLGYVWSICEGSVEEEWRHSVAPIPADKLEKVLHKAPDDEVELTQDANVLPPSGE 540
           ELGKGGLGYVWSICEGSVEEEWRHSVAPIPADKLEKVLHKAPDDEVELTQDANVLPPSGE
Sbjct: 481 ELGKGGLGYVWSICEGSVEEEWRHSVAPIPADKLEKVLHKAPDDEVELTQDANVLPPSGE 540

Query: 541 LKSDSYVSDGDDNESSRDDSIHFEKEVEATFLRAVHENIEVANVILEVNSLRLSFNKVAS 600
           LKSDSYVSDGDDNESSRDDSIHFEKEVEATFLRAVHENIEVANVILEVNSLRLSFNKVAS
Sbjct: 541 LKSDSYVSDGDDNESSRDDSIHFEKEVEATFLRAVHENIEVANVILEVNSLRLSFNKVAS 600

Query: 601 DCAGAIFYSLMNFALDSSRGSSSELLKNTTSIITKWKKLLKYYLTDLDEEIEVILKFEEI 660
           DCAGAIFYSLMNFALDSSRGSSSELLKNTTSIITKWKKLLKYYLTDLDEEIEVILKFEEI
Sbjct: 601 DCAGAIFYSLMNFALDSSRGSSSELLKNTTSIITKWKKLLKYYLTDLDEEIEVILKFEEI 660

Query: 661 CLESAKEFTPHFTKILWQLYDQEIIQEEAILKWDAEKKEADDSDKLFVKQAEKFIEWLKV 720
           CLESAKEFTPHFTKILWQLYDQEIIQEEAILKWDAEKKEADDSDKLFVKQAEKFIEWLKV
Sbjct: 661 CLESAKEFTPHFTKILWQLYDQEIIQEEAILKWDAEKKEADDSDKLFVKQAEKFIEWLKV 720

Query: 721 ASEEEEEEEEE 732
           ASEEEEEEEEE
Sbjct: 721 ASEEEEEEEEE 731

BLAST of Cucsa.133570 vs. NCBI nr
Match: gi|778666638|ref|XP_011648781.1| (PREDICTED: translation initiation factor eIF-2B subunit epsilon isoform X2 [Cucumis sativus])

HSP 1 Score: 1442.9 bits (3734), Expect = 0.0e+00
Identity = 726/731 (99.32%), Postives = 726/731 (99.32%), Query Frame = 1

Query: 1   MGAQRKGASRVSDDHEELARVSLQAVLLADSFTTKFRPITLERPKVLLPLVNVPMINYTL 60
           MGAQRKGASRVSDDHEELARVSLQAVLLADSFTTKFRPITLERPKVLLPLVNVPMINYTL
Sbjct: 1   MGAQRKGASRVSDDHEELARVSLQAVLLADSFTTKFRPITLERPKVLLPLVNVPMINYTL 60

Query: 61  SWLESAGVEEVFVFCCANSKQVIDYLENSDWVSLPDFAVRTIESHNSISAGDALRLIYER 120
           SWLESAGVEEVFVFCCANSKQVIDYLENSDWVSLPDFAVRTIESHNSISAGDALRLIYER
Sbjct: 61  SWLESAGVEEVFVFCCANSKQVIDYLENSDWVSLPDFAVRTIESHNSISAGDALRLIYER 120

Query: 121 NVIHGDFVLITGDTISNMSLTQALQEHKERKKKDSNAVMTMVVKRSKPSPITHQSRLGTD 180
           NVIHGDFVLITGDTISNMSLTQALQEHKERKKKDSNAVMTMVVKRSKPSPITHQSRLGTD
Sbjct: 121 NVIHGDFVLITGDTISNMSLTQALQEHKERKKKDSNAVMTMVVKRSKPSPITHQSRLGTD 180

Query: 181 ELFMAIDHNTKQLVYYEDRVDYSKGIIHLDKSLIMDNSSISLHNDMQDCYIDICSPEVLS 240
           ELFMAIDHNTKQLVYYEDRVDYSKGIIHLDKSLIMDNSSISLHNDMQDCYIDICSPEVLS
Sbjct: 181 ELFMAIDHNTKQLVYYEDRVDYSKGIIHLDKSLIMDNSSISLHNDMQDCYIDICSPEVLS 240

Query: 241 LFTDNFDYQHLRRHFVKGLLLDDIMGYKIYTHEICSSYAARIDNYRSYDTVSKDIIQRWT 300
           LFTDNFDYQHLRRHFVKGLLLDDIMGYKIYTHEICSSYAARIDNYRSYDTVSKDIIQRWT
Sbjct: 241 LFTDNFDYQHLRRHFVKGLLLDDIMGYKIYTHEICSSYAARIDNYRSYDTVSKDIIQRWT 300

Query: 301 YPLVPDVKFFGNSTYKLERQGMYRASEILQARSAQVGPFTVIGDNSKIGDNTKITNSIIG 360
           YPLVPDVKFFGNSTYKLERQGMYRASEILQARSAQVGPFTVIGDNSKIGDNTKITNSIIG
Sbjct: 301 YPLVPDVKFFGNSTYKLERQGMYRASEILQARSAQVGPFTVIGDNSKIGDNTKITNSIIG 360

Query: 361 HGCSIGSNVKIEGSYIWDNVTIEDGCKISNAIVCDGVVIKSGAELEPGVILSFKVVVGDQ 420
           HGCSIGSNVKIEGSYIWDNVTIEDGCKISNAIVCDGVVIKSGAELEPGVILSFKVVVGDQ
Sbjct: 361 HGCSIGSNVKIEGSYIWDNVTIEDGCKISNAIVCDGVVIKSGAELEPGVILSFKVVVGDQ 420

Query: 421 FTVPSYSKVSLFEQPTNQDSDEELEYADNSSGIVEFADTTDKSNSALMSNPLEMQGWPAS 480
           FTVPSYSKVSLFEQPTNQDSDEELEYADNSS     ADTTDKSNSALMSNPLEMQGWPAS
Sbjct: 421 FTVPSYSKVSLFEQPTNQDSDEELEYADNSS----VADTTDKSNSALMSNPLEMQGWPAS 480

Query: 481 ELGKGGLGYVWSICEGSVEEEWRHSVAPIPADKLEKVLHKAPDDEVELTQDANVLPPSGE 540
           ELGKGGLGYVWSICEGSVEEEWRHSVAPIPADKLEKVLHKAPDDEVELTQDANVLPPSGE
Sbjct: 481 ELGKGGLGYVWSICEGSVEEEWRHSVAPIPADKLEKVLHKAPDDEVELTQDANVLPPSGE 540

Query: 541 LKSDSYVSDGDDNESSRDDSIHFEKEVEATFLRAVHENIEVANVILEVNSLRLSFNKVAS 600
           LKSDSYVSDGDDNESSRDDSIHFEKEVEATFLRAVHENIEVANVILEVNSLRLSFNKVAS
Sbjct: 541 LKSDSYVSDGDDNESSRDDSIHFEKEVEATFLRAVHENIEVANVILEVNSLRLSFNKVAS 600

Query: 601 DCAGAIFYSLMNFALDSSRGSSSELLKNTTSIITKWKKLLKYYLTDLDEEIEVILKFEEI 660
           DCAGAIFYSLMNFALDSSRGSSSELLKNTTSIITKWKKLLKYYLTDLDEEIEVILKFEEI
Sbjct: 601 DCAGAIFYSLMNFALDSSRGSSSELLKNTTSIITKWKKLLKYYLTDLDEEIEVILKFEEI 660

Query: 661 CLESAKEFTPHFTKILWQLYDQEIIQEEAILKWDAEKKEADDSDKLFVKQAEKFIEWLKV 720
           CLESAKEFTPHFTKILWQLYDQEIIQEEAILKWDAEKKEADDSDKLFVKQAEKFIEWLKV
Sbjct: 661 CLESAKEFTPHFTKILWQLYDQEIIQEEAILKWDAEKKEADDSDKLFVKQAEKFIEWLKV 720

Query: 721 ASEEEEEEEEE 732
           ASEEEEEEEEE
Sbjct: 721 ASEEEEEEEEE 727

BLAST of Cucsa.133570 vs. NCBI nr
Match: gi|659070546|ref|XP_008455609.1| (PREDICTED: translation initiation factor eIF-2B subunit epsilon isoform X1 [Cucumis melo])

HSP 1 Score: 1440.6 bits (3728), Expect = 0.0e+00
Identity = 720/731 (98.50%), Postives = 727/731 (99.45%), Query Frame = 1

Query: 1   MGAQRKGASRVSDDHEELARVSLQAVLLADSFTTKFRPITLERPKVLLPLVNVPMINYTL 60
           MGAQRKGASRVSDDHEELARVSLQAVLLADSFTTKFRPITLERPKVLLPLVNVPMINYTL
Sbjct: 1   MGAQRKGASRVSDDHEELARVSLQAVLLADSFTTKFRPITLERPKVLLPLVNVPMINYTL 60

Query: 61  SWLESAGVEEVFVFCCANSKQVIDYLENSDWVSLPDFAVRTIESHNSISAGDALRLIYER 120
           SWLESAGVEEVFVFCCANSKQVIDYLENS+WVSLPDFAVRTIESHNSISAGDALRLIYER
Sbjct: 61  SWLESAGVEEVFVFCCANSKQVIDYLENSEWVSLPDFAVRTIESHNSISAGDALRLIYER 120

Query: 121 NVIHGDFVLITGDTISNMSLTQALQEHKERKKKDSNAVMTMVVKRSKPSPITHQSRLGTD 180
           NVIHGDFVLITGDTISNMSLTQALQEHKERKKKDSNAVMTMVVKRSKPSPITHQSRLGTD
Sbjct: 121 NVIHGDFVLITGDTISNMSLTQALQEHKERKKKDSNAVMTMVVKRSKPSPITHQSRLGTD 180

Query: 181 ELFMAIDHNTKQLVYYEDRVDYSKGIIHLDKSLIMDNSSISLHNDMQDCYIDICSPEVLS 240
           ELFMAIDHNTKQLVYYED+VDY+KGIIHLDKSLIMDNSSISLHNDMQDCYIDICSPEVLS
Sbjct: 181 ELFMAIDHNTKQLVYYEDKVDYAKGIIHLDKSLIMDNSSISLHNDMQDCYIDICSPEVLS 240

Query: 241 LFTDNFDYQHLRRHFVKGLLLDDIMGYKIYTHEICSSYAARIDNYRSYDTVSKDIIQRWT 300
           LFTDNFDYQHLRRHFVKGLLLDDIMGYKIYT+EICSSYAARIDNYRSYDTVSKDIIQRWT
Sbjct: 241 LFTDNFDYQHLRRHFVKGLLLDDIMGYKIYTYEICSSYAARIDNYRSYDTVSKDIIQRWT 300

Query: 301 YPLVPDVKFFGNSTYKLERQGMYRASEILQARSAQVGPFTVIGDNSKIGDNTKITNSIIG 360
           YPLVPDVKFFGNSTYKLERQGMYRASEILQARSAQVGPFTVIGDNSKIGDNTKITNSIIG
Sbjct: 301 YPLVPDVKFFGNSTYKLERQGMYRASEILQARSAQVGPFTVIGDNSKIGDNTKITNSIIG 360

Query: 361 HGCSIGSNVKIEGSYIWDNVTIEDGCKISNAIVCDGVVIKSGAELEPGVILSFKVVVGDQ 420
           HGCSIGSNVKIEGSYIWDNVTIEDGCK+S+AIVCDGVVIKSGAELEPGVILSFKVVVGDQ
Sbjct: 361 HGCSIGSNVKIEGSYIWDNVTIEDGCKLSHAIVCDGVVIKSGAELEPGVILSFKVVVGDQ 420

Query: 421 FTVPSYSKVSLFEQPTNQDSDEELEYADNSSGIVEFADTTDKSNSALMSNPLEMQGWPAS 480
           FTVPSYSKVSLFEQPTNQDSDEELEYADNSSGIVEFADTTDKSNSALMS  LEMQGWPAS
Sbjct: 421 FTVPSYSKVSLFEQPTNQDSDEELEYADNSSGIVEFADTTDKSNSALMSKSLEMQGWPAS 480

Query: 481 ELGKGGLGYVWSICEGSVEEEWRHSVAPIPADKLEKVLHKAPDDEVELTQDANVLPPSGE 540
           ELGKGGLGYVWSICEGSVEEEWRHSVAPIPADKLEKVLHKAPDDEVELTQDANVLPPSGE
Sbjct: 481 ELGKGGLGYVWSICEGSVEEEWRHSVAPIPADKLEKVLHKAPDDEVELTQDANVLPPSGE 540

Query: 541 LKSDSYVSDGDDNESSRDDSIHFEKEVEATFLRAVHENIEVANVILEVNSLRLSFNKVAS 600
           LKSDSY+SDGDDNESSRDDSIHFEKEVEATFLRAVHENIEVANVILEVNSLRLSFNKVAS
Sbjct: 541 LKSDSYISDGDDNESSRDDSIHFEKEVEATFLRAVHENIEVANVILEVNSLRLSFNKVAS 600

Query: 601 DCAGAIFYSLMNFALDSSRGSSSELLKNTTSIITKWKKLLKYYLTDLDEEIEVILKFEEI 660
           DCAGAIFYSLMNFALDSSRGSS ELLKNTTSIITKWKKLLKYYLTDLDEEIEVILKFEEI
Sbjct: 601 DCAGAIFYSLMNFALDSSRGSSGELLKNTTSIITKWKKLLKYYLTDLDEEIEVILKFEEI 660

Query: 661 CLESAKEFTPHFTKILWQLYDQEIIQEEAILKWDAEKKEADDSDKLFVKQAEKFIEWLKV 720
           CLESAKEFTPHFTKILW LYDQEIIQEEAILKWDAEKKEADDSDKLFVKQAEKFIEWLKV
Sbjct: 661 CLESAKEFTPHFTKILWHLYDQEIIQEEAILKWDAEKKEADDSDKLFVKQAEKFIEWLKV 720

Query: 721 ASEEEEEEEEE 732
           ASEEEEEEEEE
Sbjct: 721 ASEEEEEEEEE 731

BLAST of Cucsa.133570 vs. NCBI nr
Match: gi|659070548|ref|XP_008455617.1| (PREDICTED: translation initiation factor eIF-2B subunit epsilon isoform X2 [Cucumis melo])

HSP 1 Score: 1424.8 bits (3687), Expect = 0.0e+00
Identity = 715/731 (97.81%), Postives = 722/731 (98.77%), Query Frame = 1

Query: 1   MGAQRKGASRVSDDHEELARVSLQAVLLADSFTTKFRPITLERPKVLLPLVNVPMINYTL 60
           MGAQRKGASRVSDDHEELARVSLQAVLLADSFTTKFRPITLERPKVLLPLVNVPMINYTL
Sbjct: 1   MGAQRKGASRVSDDHEELARVSLQAVLLADSFTTKFRPITLERPKVLLPLVNVPMINYTL 60

Query: 61  SWLESAGVEEVFVFCCANSKQVIDYLENSDWVSLPDFAVRTIESHNSISAGDALRLIYER 120
           SWLESAGVEEVFVFCCANSKQVIDYLENS+WVSLPDFAVRTIESHNSISAGDALRLIYER
Sbjct: 61  SWLESAGVEEVFVFCCANSKQVIDYLENSEWVSLPDFAVRTIESHNSISAGDALRLIYER 120

Query: 121 NVIHGDFVLITGDTISNMSLTQALQEHKERKKKDSNAVMTMVVKRSKPSPITHQSRLGTD 180
           NVIHGDFVLITGDTISNMSLTQALQEHKERKKKDSNAVMTMVVKRSKPSPITHQSRLGTD
Sbjct: 121 NVIHGDFVLITGDTISNMSLTQALQEHKERKKKDSNAVMTMVVKRSKPSPITHQSRLGTD 180

Query: 181 ELFMAIDHNTKQLVYYEDRVDYSKGIIHLDKSLIMDNSSISLHNDMQDCYIDICSPEVLS 240
           ELFMAIDHNTKQLVYYED+VDY+KGIIHLDKSLIMDNSSISLHNDMQDCYIDICSPEVLS
Sbjct: 181 ELFMAIDHNTKQLVYYEDKVDYAKGIIHLDKSLIMDNSSISLHNDMQDCYIDICSPEVLS 240

Query: 241 LFTDNFDYQHLRRHFVKGLLLDDIMGYKIYTHEICSSYAARIDNYRSYDTVSKDIIQRWT 300
           LFTDNFDYQHLRRHFVKGLLLDDIMGYKIYT+EICSSYAARIDNYRSYDTVSKDIIQRWT
Sbjct: 241 LFTDNFDYQHLRRHFVKGLLLDDIMGYKIYTYEICSSYAARIDNYRSYDTVSKDIIQRWT 300

Query: 301 YPLVPDVKFFGNSTYKLERQGMYRASEILQARSAQVGPFTVIGDNSKIGDNTKITNSIIG 360
           YPLVPDVKFFGNSTYKLERQGMYRASEILQARSAQVGPFTVIGDNSKIGDNTKITNSIIG
Sbjct: 301 YPLVPDVKFFGNSTYKLERQGMYRASEILQARSAQVGPFTVIGDNSKIGDNTKITNSIIG 360

Query: 361 HGCSIGSNVKIEGSYIWDNVTIEDGCKISNAIVCDGVVIKSGAELEPGVILSFKVVVGDQ 420
           HGCSIGSNVKIEGSYIWDNVTIEDGCK+S+AIVCDGVVIKSGAELEPGVILSFKVVVGDQ
Sbjct: 361 HGCSIGSNVKIEGSYIWDNVTIEDGCKLSHAIVCDGVVIKSGAELEPGVILSFKVVVGDQ 420

Query: 421 FTVPSYSKVSLFEQPTNQDSDEELEYADNSSGIVEFADTTDKSNSALMSNPLEMQGWPAS 480
           FTVPSYSKVSLFEQPTNQDSDEELEYADNSS     ADTTDKSNSALMS  LEMQGWPAS
Sbjct: 421 FTVPSYSKVSLFEQPTNQDSDEELEYADNSS----VADTTDKSNSALMSKSLEMQGWPAS 480

Query: 481 ELGKGGLGYVWSICEGSVEEEWRHSVAPIPADKLEKVLHKAPDDEVELTQDANVLPPSGE 540
           ELGKGGLGYVWSICEGSVEEEWRHSVAPIPADKLEKVLHKAPDDEVELTQDANVLPPSGE
Sbjct: 481 ELGKGGLGYVWSICEGSVEEEWRHSVAPIPADKLEKVLHKAPDDEVELTQDANVLPPSGE 540

Query: 541 LKSDSYVSDGDDNESSRDDSIHFEKEVEATFLRAVHENIEVANVILEVNSLRLSFNKVAS 600
           LKSDSY+SDGDDNESSRDDSIHFEKEVEATFLRAVHENIEVANVILEVNSLRLSFNKVAS
Sbjct: 541 LKSDSYISDGDDNESSRDDSIHFEKEVEATFLRAVHENIEVANVILEVNSLRLSFNKVAS 600

Query: 601 DCAGAIFYSLMNFALDSSRGSSSELLKNTTSIITKWKKLLKYYLTDLDEEIEVILKFEEI 660
           DCAGAIFYSLMNFALDSSRGSS ELLKNTTSIITKWKKLLKYYLTDLDEEIEVILKFEEI
Sbjct: 601 DCAGAIFYSLMNFALDSSRGSSGELLKNTTSIITKWKKLLKYYLTDLDEEIEVILKFEEI 660

Query: 661 CLESAKEFTPHFTKILWQLYDQEIIQEEAILKWDAEKKEADDSDKLFVKQAEKFIEWLKV 720
           CLESAKEFTPHFTKILW LYDQEIIQEEAILKWDAEKKEADDSDKLFVKQAEKFIEWLKV
Sbjct: 661 CLESAKEFTPHFTKILWHLYDQEIIQEEAILKWDAEKKEADDSDKLFVKQAEKFIEWLKV 720

Query: 721 ASEEEEEEEEE 732
           ASEEEEEEEEE
Sbjct: 721 ASEEEEEEEEE 727

BLAST of Cucsa.133570 vs. NCBI nr
Match: gi|659070550|ref|XP_008455622.1| (PREDICTED: translation initiation factor eIF-2B subunit epsilon isoform X3 [Cucumis melo])

HSP 1 Score: 1173.7 bits (3035), Expect = 0.0e+00
Identity = 584/594 (98.32%), Postives = 590/594 (99.33%), Query Frame = 1

Query: 138 MSLTQALQEHKERKKKDSNAVMTMVVKRSKPSPITHQSRLGTDELFMAIDHNTKQLVYYE 197
           MSLTQALQEHKERKKKDSNAVMTMVVKRSKPSPITHQSRLGTDELFMAIDHNTKQLVYYE
Sbjct: 1   MSLTQALQEHKERKKKDSNAVMTMVVKRSKPSPITHQSRLGTDELFMAIDHNTKQLVYYE 60

Query: 198 DRVDYSKGIIHLDKSLIMDNSSISLHNDMQDCYIDICSPEVLSLFTDNFDYQHLRRHFVK 257
           D+VDY+KGIIHLDKSLIMDNSSISLHNDMQDCYIDICSPEVLSLFTDNFDYQHLRRHFVK
Sbjct: 61  DKVDYAKGIIHLDKSLIMDNSSISLHNDMQDCYIDICSPEVLSLFTDNFDYQHLRRHFVK 120

Query: 258 GLLLDDIMGYKIYTHEICSSYAARIDNYRSYDTVSKDIIQRWTYPLVPDVKFFGNSTYKL 317
           GLLLDDIMGYKIYT+EICSSYAARIDNYRSYDTVSKDIIQRWTYPLVPDVKFFGNSTYKL
Sbjct: 121 GLLLDDIMGYKIYTYEICSSYAARIDNYRSYDTVSKDIIQRWTYPLVPDVKFFGNSTYKL 180

Query: 318 ERQGMYRASEILQARSAQVGPFTVIGDNSKIGDNTKITNSIIGHGCSIGSNVKIEGSYIW 377
           ERQGMYRASEILQARSAQVGPFTVIGDNSKIGDNTKITNSIIGHGCSIGSNVKIEGSYIW
Sbjct: 181 ERQGMYRASEILQARSAQVGPFTVIGDNSKIGDNTKITNSIIGHGCSIGSNVKIEGSYIW 240

Query: 378 DNVTIEDGCKISNAIVCDGVVIKSGAELEPGVILSFKVVVGDQFTVPSYSKVSLFEQPTN 437
           DNVTIEDGCK+S+AIVCDGVVIKSGAELEPGVILSFKVVVGDQFTVPSYSKVSLFEQPTN
Sbjct: 241 DNVTIEDGCKLSHAIVCDGVVIKSGAELEPGVILSFKVVVGDQFTVPSYSKVSLFEQPTN 300

Query: 438 QDSDEELEYADNSSGIVEFADTTDKSNSALMSNPLEMQGWPASELGKGGLGYVWSICEGS 497
           QDSDEELEYADNSSGIVEFADTTDKSNSALMS  LEMQGWPASELGKGGLGYVWSICEGS
Sbjct: 301 QDSDEELEYADNSSGIVEFADTTDKSNSALMSKSLEMQGWPASELGKGGLGYVWSICEGS 360

Query: 498 VEEEWRHSVAPIPADKLEKVLHKAPDDEVELTQDANVLPPSGELKSDSYVSDGDDNESSR 557
           VEEEWRHSVAPIPADKLEKVLHKAPDDEVELTQDANVLPPSGELKSDSY+SDGDDNESSR
Sbjct: 361 VEEEWRHSVAPIPADKLEKVLHKAPDDEVELTQDANVLPPSGELKSDSYISDGDDNESSR 420

Query: 558 DDSIHFEKEVEATFLRAVHENIEVANVILEVNSLRLSFNKVASDCAGAIFYSLMNFALDS 617
           DDSIHFEKEVEATFLRAVHENIEVANVILEVNSLRLSFNKVASDCAGAIFYSLMNFALDS
Sbjct: 421 DDSIHFEKEVEATFLRAVHENIEVANVILEVNSLRLSFNKVASDCAGAIFYSLMNFALDS 480

Query: 618 SRGSSSELLKNTTSIITKWKKLLKYYLTDLDEEIEVILKFEEICLESAKEFTPHFTKILW 677
           SRGSS ELLKNTTSIITKWKKLLKYYLTDLDEEIEVILKFEEICLESAKEFTPHFTKILW
Sbjct: 481 SRGSSGELLKNTTSIITKWKKLLKYYLTDLDEEIEVILKFEEICLESAKEFTPHFTKILW 540

Query: 678 QLYDQEIIQEEAILKWDAEKKEADDSDKLFVKQAEKFIEWLKVASEEEEEEEEE 732
            LYDQEIIQEEAILKWDAEKKEADDSDKLFVKQAEKFIEWLKVASEEEEEEEEE
Sbjct: 541 HLYDQEIIQEEAILKWDAEKKEADDSDKLFVKQAEKFIEWLKVASEEEEEEEEE 594