Cucsa.128130 (gene) Cucumber (Gy14) v1

BLAST of Cucsa.128130 vs. Swiss-Prot
Match: RGA3_SOLBU (Putative disease resistance protein RGA3 OS=Solanum bulbocastanum GN=RGA3 PE=2 SV=2)

HSP 1 Score: 394.8 bits (1013), Expect = 2.5e-108
Identity = 279/863 (32.33%), Postives = 458/863 (53.07%), Query Frame = 1

Query: 33  LGMVCGLNDDLNKLRSNVSSIQSVLRDAEQRQIKGNDHSLTDWLEKLGDVFYDVEDVLDE 92
           LG+V G   +  KL S  S IQ+VL DA+++Q+K    ++ +WL+KL    Y+V+D+LD+
Sbjct: 21  LGLVFGFEKEFKKLSSMFSMIQAVLEDAQEKQLKYK--AIKNWLQKLNVAAYEVDDILDD 80

Query: 93  ISTEALRREVMTRGKNAKQVRIFFSNSNQLAFNYRMACQVKKINERLDVISQEKDKFQLN 152
             TEA R +    G+          +   + F Y++  ++K++ E+LD I++E+  F L+
Sbjct: 81  CKTEAARFKQAVLGRY---------HPRTITFCYKVGKRMKEMMEKLDAIAEERRNFHLD 140

Query: 153 GIAYLGIQNVLSYPIGMERDTHSSLSGDQKIIGRDDEMNNLKKNLLAEDDKVKANVSFIA 212
                  + ++       R T   L+ + K+ GR+ E + + K +L  +      V  + 
Sbjct: 141 -------ERIIERQAA-RRQTGFVLT-EPKVYGREKEEDEIVK-ILINNVSYSEEVPVLP 200

Query: 213 IVGMGGIGKTTLAKSLYNDKQVSDGFSSRIWIWVSNQFDTKTILKKIIESATEKKPKVEE 272
           I+GMGG+GKTTLA+ ++ND+++++ F+ +IW+ VS+ FD K ++K I+ES   K     +
Sbjct: 201 ILGMGGLGKTTLAQMVFNDQRITEHFNLKIWVCVSDDFDEKRLIKAIVESIEGKSLGDMD 260

Query: 273 MEPLKTKLEEVIGGKKYLLVMDDVWNENENEWENLKNLLMLGARGSKVLITKRDSK--AV 332
           + PL+ KL+E++ GK+Y LV+DDVWNE++ +W+NL+ +L +GA G+ +LIT R  K  ++
Sbjct: 261 LAPLQKKLQELLNGKRYFLVLDDVWNEDQEKWDNLRAVLKIGASGASILITTRLEKIGSI 320

Query: 333 PG-VETIPLKDLTEDFSWLLFKEVAFKESDLESINQNLIKMGKEISKRCGGIPLVIRHIG 392
            G ++   L +L+++  WLLFK+ AF        +  L+++GKEI K+CGG+PL  + +G
Sbjct: 321 MGTLQLYQLSNLSQEDCWLLFKQRAFCHQ--TETSPKLMEIGKEIVKKCGGVPLAAKTLG 380

Query: 393 RLLYGKTSAEDWEFIKENELLNVTREKNNNDGHVISTLKLSYNHLSPNLKQCFSYSSLFP 452
            LL  K    +WE ++++E+ N+ +++N+    V+  L+LSY+HL  +L+QCF+Y ++FP
Sbjct: 381 GLLRFKREESEWEHVRDSEIWNLPQDENS----VLPALRLSYHHLPLDLRQCFAYCAVFP 440

Query: 453 KGYKIRMNELIRQWIAQGFIESSNGGKSVENIGKEYLDELCWR-FFYEISIEDVPFEEVG 512
           K  KI    LI  W+A  F+  S G   +E++G E  +EL  R FF EI ++        
Sbjct: 441 KDTKIEKEYLIALWMAHSFL-LSKGNMELEDVGNEVWNELYLRSFFQEIEVKSGK-TYFK 500

Query: 513 MHDLMCDLAREVAGQKLYIRGYPESGYVVSEQTRHISFEYEPRSWIDDVSKLQQAKGLRT 572
           MHDL+ DLA  +       R   +      E    I   Y+      D+  +  ++ + +
Sbjct: 501 MHDLIHDLATSMFSASASSRSIRQINVKDDEDMMFIVTNYK------DMMSIGFSEVVSS 560

Query: 573 FLLFTKNPFFTRNPIEKVLLDRLFSHFPRLRVLQIPN-----VSKSIKKLRHLRYLEL-G 632
           +                     LF  F  LRVL + N     +  S+  L HLRYL+L G
Sbjct: 561 Y------------------SPSLFKRFVSLRVLNLSNSEFEQLPSSVGDLVHLRYLDLSG 620

Query: 633 EDAKSVPNSITKLQNLQTLDLTKCYDLKELPRDINNFVNLRHLLCDSRLMNMLQGTMEKL 692
               S+P  + KLQNLQTLDL  C  L  LP+  +   +LR+L+ D   +  +   +  L
Sbjct: 621 NKICSLPKRLCKLQNLQTLDLYNCQSLSCLPKQTSKLCSLRNLVLDHCPLTSMPPRIGLL 680

Query: 693 TSLQTLSSFLFDCKRFDKVKEFSERSYFIEFDLKIKGLEQLRFSPSDVKSVNLKNKKVPL 752
           T L+TL  F+   ++  ++ E   R+  +   + I  LE+++ +  + K  NL + K  L
Sbjct: 681 TCLKTLGYFVVGERKGYQLGEL--RNLNLRGAISITHLERVK-NDMEAKEANL-SAKANL 740

Query: 753 LRLKWKFENGNEYEGDADDIVLEGLEPHPYVNLLQIEGYCGVGLPNWVSTSIL--LRGIR 812
             L   ++  N YE + +  VLE L+PHP +  L+I  +CG  LP+W++ S+L  +  I 
Sbjct: 741 HSLSMSWDRPNRYESE-EVKVLEALKPHPNLKYLEIIDFCGFCLPDWMNHSVLKNVVSIL 800

Query: 813 IGNCDRLH-LNQLSHLHALEILNLEGLKSVMSISEWIGTLT-----SLVSLEIEECPKLK 872
           I  C+    L     L  LE L L+     +   E  G LT     SL  L I     LK
Sbjct: 801 ISGCENCSCLPPFGELPCLESLELQDGSVEVEYVEDSGFLTRRRFPSLRKLHIGGFCNLK 825

Query: 873 SLP--KEMQQLKSLVQLNIIKCP 876
            L   K  +Q   L ++ I  CP
Sbjct: 861 GLQRMKGAEQFPVLEEMKISDCP 825

HSP 2 Score: 68.6 bits (166), Expect = 4.1e-10
Identity = 41/100 (41.00%), Postives = 57/100 (57.00%), Query Frame = 1

Query: 801 IGNCDRLHLNQLSHLHALEILNLEGLKSVMSISEWIGTLTSLVSLEIEECPKLKSLPKEM 860
           + + + L    + + +ALE L  EGL+           L+SL  L +E C  LK LP+ +
Sbjct: 901 LASLNNLKCLDIRYCYALESLPEEGLEG----------LSSLTELFVEHCNMLKCLPEGL 960

Query: 861 QQLKSLVQLNIIKCPQLGERCKEG-GEDWPNISHIPDVLI 900
           Q L +L  L I  CPQL +RC++G GEDW  ISHIP+V I
Sbjct: 961 QHLTTLTSLKIRGCPQLIKRCEKGIGEDWHKISHIPNVNI 990

HSP 3 Score: 44.7 bits (104), Expect = 6.3e-03
Identity = 30/88 (34.09%), Postives = 46/88 (52.27%), Query Frame = 1

Query: 606 VSKSIKKLRHLRYLELG--EDAKSVPNSITKLQNLQTLDLTKCYDLKELPRD-INNFVNL 665
           + +  K L +L YL +   E+ K +P S+  L NL+ LD+  CY L+ LP + +    +L
Sbjct: 873 LEEMFKNLENLIYLSVSFLENLKELPTSLASLNNLKCLDIRYCYALESLPEEGLEGLSSL 932

Query: 666 RHLLCDSRLMNMLQGTMEKLTSLQTLSS 691
             L  +    NML+   E L  L TL+S
Sbjct: 933 TELFVEH--CNMLKCLPEGLQHLTTLTS 958

BLAST of Cucsa.128130 vs. Swiss-Prot
Match: RGA1_SOLBU (Putative disease resistance protein RGA1 OS=Solanum bulbocastanum GN=RGA1 PE=2 SV=2)

HSP 1 Score: 381.7 bits (979), Expect = 2.2e-104
Identity = 275/863 (31.87%), Postives = 451/863 (52.26%), Query Frame = 1

Query: 33  LGMVCGLNDDLNKLRSNVSSIQSVLRDAEQRQIKGNDHSLTDWLEKLGDVFYDVEDVLDE 92
           L ++ G  D+  +L S  S+IQ+VL DA+++Q+  ND  L +WL+KL    Y+V+D+LDE
Sbjct: 21  LVLLFGFQDEFQRLSSMFSTIQAVLEDAQEKQL--NDKPLENWLQKLNAATYEVDDILDE 80

Query: 93  ISTEALRREVMTRGKNAKQVRIFFSNSNQLAFNYRMACQVKKINERLDVISQEKDKFQLN 152
             T+A R      G+   +V         + F +++  ++ ++ ++L+ I++E+ KF L 
Sbjct: 81  YKTKATRFLQSEYGRYHPKV---------IPFRHKVGKRMDQVMKKLNAIAEERKKFHL- 140

Query: 153 GIAYLGIQNVLSYPIGMERDTHSSLSGDQKIIGRDDEMNNLKKNLLAEDDKVKANVSFIA 212
                  Q  +       R+T S L+  Q + GRD E + + K L+      +  +S + 
Sbjct: 141 -------QEKIIERQAATRETGSVLTEPQ-VYGRDKEKDEIVKILINTASDAQ-KLSVLP 200

Query: 213 IVGMGGIGKTTLAKSLYNDKQVSDGFSSRIWIWVSNQFDTKTILKKIIESATEKKPKVEE 272
           I+GMGG+GKTTL++ ++ND++V++ F  +IWI +S+ F+ K ++K I+ES   K     +
Sbjct: 201 ILGMGGLGKTTLSQMVFNDQRVTERFYPKIWICISDDFNEKRLIKAIVESIEGKSLSDMD 260

Query: 273 MEPLKTKLEEVIGGKKYLLVMDDVWNENENEWENLKNLLMLGARGSKVLITKRDSKA--- 332
           + PL+ KL+E++ GK+Y LV+DDVWNE++++W NL+ +L +GA G+ VL T R  K    
Sbjct: 261 LAPLQKKLQELLNGKRYFLVLDDVWNEDQHKWANLRAVLKVGASGAFVLTTTRLEKVGSI 320

Query: 333 VPGVETIPLKDLTEDFSWLLFKEVAFKESDLESINQNLIKMGKEISKRCGGIPLVIRHIG 392
           +  ++   L +L+ +  W LF + AF     E IN NL+ +GKEI K+CGG+PL  + +G
Sbjct: 321 MGTLQPYELSNLSPEDCWFLFMQRAFGHQ--EEINPNLMAIGKEIVKKCGGVPLAAKTLG 380

Query: 393 RLLYGKTSAEDWEFIKENELLNVTREKNNNDGHVISTLKLSYNHLSPNLKQCFSYSSLFP 452
            +L  K    +WE ++++ + N+ +++++    ++  L+LSY+HL  +L+QCF Y ++FP
Sbjct: 381 GILRFKREEREWEHVRDSPIWNLPQDESS----ILPALRLSYHHLPLDLRQCFVYCAVFP 440

Query: 453 KGYKIRMNELIRQWIAQGFIESSNGGKSVENIGKEYLDELCWR-FFYEISIEDVPFEEVG 512
           K  K+    LI  W+A GF+  S G   +E++G E  +EL  R FF EI +E        
Sbjct: 441 KDTKMAKENLIAFWMAHGFL-LSKGNLELEDVGNEVWNELYLRSFFQEIEVESGK-TYFK 500

Query: 513 MHDLMCDLAREVAGQKLYIRGYPE-----SGYVVSEQTRHISFEYEPRSWIDDVSKLQQA 572
           MHDL+ DLA  +           E      GY++S     +   Y P       S LQ+ 
Sbjct: 501 MHDLIHDLATSLFSANTSSSNIREINANYDGYMMSIGFAEVVSSYSP-------SLLQKF 560

Query: 573 KGLRTFLLFTKNPFFTRNPIEKVLLDRLFSHFPRLRVLQIPNVSKSIKKLRHLRYLELGE 632
             LR   L   N                        + Q+P+   SI  L HLRYL+L  
Sbjct: 561 VSLRVLNLRNSN------------------------LNQLPS---SIGDLVHLRYLDLSG 620

Query: 633 D--AKSVPNSITKLQNLQTLDLTKCYDLKELPRDINNFVNLRHLLCDSRLMNMLQGTMEK 692
           +   +++P  + KLQNLQTLDL  C  L  LP+  +   +LR+LL D   +      +  
Sbjct: 621 NFRIRNLPKRLCKLQNLQTLDLHYCDSLSCLPKQTSKLGSLRNLLLDGCSLTSTPPRIGL 680

Query: 693 LTSLQTLSSFLFDCKRFDKVKEFSERSYFIEFDLKIKGLEQLRFSPSDVKSVNLKNK-KV 752
           LT L++LS F+   ++  ++ E    + +    + I  L++++   +D K  NL  K  +
Sbjct: 681 LTCLKSLSCFVIGKRKGHQLGELKNLNLY--GSISITKLDRVK-KDTDAKEANLSAKANL 740

Query: 753 PLLRLKWKFENGNEYEGDADDIVLEGLEPHPYVNLLQIEGYCGVGLPNWVSTSIL--LRG 812
             L L W  +  + Y+ +    VLE L+PH  +  L+I G+ G+ LP+W++ S+L  +  
Sbjct: 741 HSLCLSWDLDGKHRYDSE----VLEALKPHSNLKYLEINGFGGIRLPDWMNQSVLKNVVS 800

Query: 813 IRIGNCDRLH-LNQLSHLHALEILNLE-GLKSVMSISEWI--GTLTSLVSLEIEECPKLK 872
           IRI  C+    L     L  LE L L  G   V  + + +  G   SL  L I +   LK
Sbjct: 801 IRIRGCENCSCLPPFGELPCLESLELHTGSADVEYVEDNVHPGRFPSLRKLVIWDFSNLK 813

Query: 873 SLPK--EMQQLKSLVQLNIIKCP 876
            L K    +Q   L ++    CP
Sbjct: 861 GLLKMEGEKQFPVLEEMTFYWCP 813

HSP 2 Score: 50.1 bits (118), Expect = 1.5e-04
Identity = 31/104 (29.81%), Postives = 50/104 (48.08%), Query Frame = 1

Query: 818 LEILNLEGLKSVMSISEWIGTLTSLVSLEIEECPKLKSLPKE------------------ 877
           L+ L +   +++  +   + +L +L SL+ E C  L+SLP+E                  
Sbjct: 870 LKYLKISFFRNLKELPTSLASLNALKSLKFEFCDALESLPEEGVKGLTSLTELSVSNCMM 929

Query: 878 -------MQQLKSLVQLNIIKCPQLGERCKEG-GEDWPNISHIP 896
                  +Q L +L  L I +CP + +RC+ G GEDW  I+HIP
Sbjct: 930 LKCLPEGLQHLTALTTLTITQCPIVFKRCERGIGEDWHKIAHIP 973

BLAST of Cucsa.128130 vs. Swiss-Prot
Match: RGA2_SOLBU (Disease resistance protein RGA2 OS=Solanum bulbocastanum GN=RGA2 PE=1 SV=1)

HSP 1 Score: 379.0 bits (972), Expect = 1.4e-103
Identity = 287/875 (32.80%), Postives = 472/875 (53.94%), Query Frame = 1

Query: 33  LGMVCGLNDDLNKLRSNVSSIQSVLRDAEQRQIKGNDHSLTDWLEKLGDVFYDVEDVLDE 92
           L ++ G  D+  +L S  S+IQ+VL DA+++Q+  N+  L +WL+KL    Y+V+D+LDE
Sbjct: 21  LVLLFGFQDEFQRLSSMFSTIQAVLEDAQEKQL--NNKPLENWLQKLNAATYEVDDILDE 80

Query: 93  ISTEALRREVMTRGKNAKQVRIFFSNSNQLAFNYRMACQVKKINERLDVISQEKDKFQLN 152
             T+A R      G+   +V         + F +++  ++ ++ ++L  I++E+  F L+
Sbjct: 81  YKTKATRFSQSEYGRYHPKV---------IPFRHKVGKRMDQVMKKLKAIAEERKNFHLH 140

Query: 153 GIAYLGIQNVLSYPIGMERDTHSSLSGDQKIIGRDDEMNNLKKNLLAEDDKVKANVSFIA 212
                  + ++     + R+T S L+  Q + GRD E + + K L+      + ++S + 
Sbjct: 141 -------EKIVERQ-AVRRETGSVLTEPQ-VYGRDKEKDEIVKILINNVSDAQ-HLSVLP 200

Query: 213 IVGMGGIGKTTLAKSLYNDKQVSDGFSSRIWIWVSNQFDTKTILKKIIESATEKKPKVEE 272
           I+GMGG+GKTTLA+ ++ND++V++ F S+IWI VS  FD K ++K I+ES  E +P + E
Sbjct: 201 ILGMGGLGKTTLAQMVFNDQRVTEHFHSKIWICVSEDFDEKRLIKAIVESI-EGRPLLGE 260

Query: 273 ME--PLKTKLEEVIGGKKYLLVMDDVWNENENEWENLKNLLMLGARGSKVLITKRDSKA- 332
           M+  PL+ KL+E++ GK+YLLV+DDVWNE++ +W NL+ +L +GA G+ VL T R  K  
Sbjct: 261 MDLAPLQKKLQELLNGKRYLLVLDDVWNEDQQKWANLRAVLKVGASGASVLTTTRLEKVG 320

Query: 333 --VPGVETIPLKDLTEDFSWLLFKEVAFKESDLESINQNLIKMGKEISKRCGGIPLVIRH 392
             +  ++   L +L+++  WLLF + AF     E IN NL+ +GKEI K+ GG+PL  + 
Sbjct: 321 SIMGTLQPYELSNLSQEDCWLLFMQRAFGHQ--EEINPNLVAIGKEIVKKSGGVPLAAKT 380

Query: 393 IGRLLYGKTSAEDWEFIKENELLNVTREKNNNDGHVISTLKLSYNHLSPNLKQCFSYSSL 452
           +G +L  K     WE ++++ + N+ +++++    ++  L+LSY+ L  +LKQCF+Y ++
Sbjct: 381 LGGILCFKREERAWEHVRDSPIWNLPQDESS----ILPALRLSYHQLPLDLKQCFAYCAV 440

Query: 453 FPKGYKIRMNELIRQWIAQGFIESSNGGKSVENIGKEYLDELCWR-FFYEISIEDVPFEE 512
           FPK  K+   +LI  W+A GF+  S G   +E++G E   EL  R FF EI ++D     
Sbjct: 441 FPKDAKMEKEKLISLWMAHGFL-LSKGNMELEDVGDEVWKELYLRSFFQEIEVKDGK-TY 500

Query: 513 VGMHDLMCDLAREVAGQKLYIRGYPESGYVVSEQTRHISFEYEPRSWIDDVSKLQQAKGL 572
             MHDL+ DLA  +            S   + E  +H S+ +       +V         
Sbjct: 501 FKMHDLIHDLATSLFSAN-------TSSSNIREINKH-SYTHMMSIGFAEV--------- 560

Query: 573 RTFLLFTKNPFFTRNPIEKVLLDRLFSHFPRLRVLQIPN-----VSKSIKKLRHLRYLEL 632
               +F    F+T  P+EK         F  LRVL + +     +  SI  L HLRYL L
Sbjct: 561 ----VF----FYTLPPLEK---------FISLRVLNLGDSTFNKLPSSIGDLVHLRYLNL 620

Query: 633 -GEDAKSVPNSITKLQNLQTLDLTKCYDLKELPRDINNFVNLRHLLCD-SRLMNMLQGTM 692
            G   +S+P  + KLQNLQTLDL  C  L  LP++ +   +LR+LL D S+ +  +   +
Sbjct: 621 YGSGMRSLPKQLCKLQNLQTLDLQYCTKLCCLPKETSKLGSLRNLLLDGSQSLTCMPPRI 680

Query: 693 EKLTSLQTLSSFLFDCKRFDKVKEFSERSYFIEFDLKIKGLEQLRFSPSDVKSVNLKNK- 752
             LT L+TL  F+   K+  ++ E    + +    +KI  LE+++ +  D K  NL  K 
Sbjct: 681 GSLTCLKTLGQFVVGRKKGYQLGELGNLNLY--GSIKISHLERVK-NDKDAKEANLSAKG 740

Query: 753 KVPLLRLKWKFENGNEYEGDADDIVLEGLEPHPYVNLLQIEGYCGVGLPNWVSTSILLRG 812
            +  L + W     + YE + +  VLE L+PH  +  L+I G+ G+ LP W++ S+L   
Sbjct: 741 NLHSLSMSWNNFGPHIYESE-EVKVLEALKPHSNLTSLKIYGFRGIHLPEWMNHSVLKNI 800

Query: 813 IRI-----GNCDRL-HLNQLSHLHALEI----LNLEGLKSV-MSISEWIGT---LTSLVS 872
           + I      NC  L     L  L +LE+     ++E ++ V + +     T     SL  
Sbjct: 801 VSILISNFRNCSCLPPFGDLPCLESLELHWGSADVEYVEEVDIDVHSGFPTRIRFPSLRK 827

Query: 873 LEIEECPKLKSLPKE--MQQLKSLVQLNIIKCPQL 878
           L+I +   LK L K+   +Q   L ++ I +CP L
Sbjct: 861 LDIWDFGSLKGLLKKEGEEQFPVLEEMIIHECPFL 827

HSP 2 Score: 70.5 bits (171), Expect = 1.1e-10
Identity = 44/108 (40.74%), Postives = 65/108 (60.19%), Query Frame = 1

Query: 796 LRGIRIGNCDRLHL--NQLSHLHALEILNLEGLKSVMSI-SEWIGTLTSLVSLEIEECPK 855
           L+ + I  C+ L      L+ L+AL+ L ++   ++ S+  E +  L+SL  L +E C  
Sbjct: 861 LKYLTISRCNNLKELPTSLASLNALKSLKIQLCCALESLPEEGLEGLSSLTELFVEHCNM 920

Query: 856 LKSLPKEMQQLKSLVQLNIIKCPQLGERCKEG-GEDWPNISHIPDVLI 900
           LK LP+ +Q L +L  L I  CPQL +RC++G GEDW  ISHIP+V I
Sbjct: 921 LKCLPEGLQHLTTLTSLKIRGCPQLIKRCEKGIGEDWHKISHIPNVNI 968

HSP 3 Score: 34.7 bits (78), Expect = 6.6e+00
Identity = 37/122 (30.33%), Postives = 61/122 (50.00%), Query Frame = 1

Query: 606 VSKSIKKLRHLRYLELGEDAKSVPNSITK-LQNLQTLDLTKCYDLKELP---RDINNFVN 665
           +S +++ L  LR     + A S P  + K L NL+ L +++C +LKELP     +N   +
Sbjct: 829 LSSNLRALTSLRIC-YNKVATSFPEEMFKNLANLKYLTISRCNNLKELPTSLASLNALKS 888

Query: 666 LRHLLCDSRLMNMLQGTMEKLTSLQTLSSFLFDCKRFDKVKEFSERSYFIEFDLKIKGLE 724
           L+  LC   L ++ +  +E L+SL  L  F+  C     + E  +    +   LKI+G  
Sbjct: 889 LKIQLC-CALESLPEEGLEGLSSLTEL--FVEHCNMLKCLPEGLQHLTTLT-SLKIRGCP 945

BLAST of Cucsa.128130 vs. Swiss-Prot
Match: RGA4_SOLBU (Putative disease resistance protein RGA4 OS=Solanum bulbocastanum GN=RGA4 PE=2 SV=1)

HSP 1 Score: 370.5 bits (950), Expect = 5.1e-101
Identity = 262/871 (30.08%), Postives = 448/871 (51.44%), Query Frame = 1

Query: 22  IKKLSSHALECLGMVCGLNDDLNKLRSNVSSIQSVLRDAEQRQIKGNDHSLTDWLEKLGD 81
           ++ L+S   + L ++ G   +  KL S  S+IQ+VL+DA+++Q+K  D ++ +WL+KL  
Sbjct: 10  LENLTSFIGDKLVLIFGFEKECEKLSSVFSTIQAVLQDAQEKQLK--DKAIENWLQKLNS 69

Query: 82  VFYDVEDVLDEISTEALRREVMTRGKNAKQVRIFFSNSNQLAFNYRMACQVKKINERLDV 141
             Y+V+D+L E   EA+R E         Q R+ F +   + F +++  ++K+I E+LD 
Sbjct: 70  AAYEVDDILGECKNEAIRFE---------QSRLGFYHPGIINFRHKIGRRMKEIMEKLDA 129

Query: 142 ISQEKDKFQLNGIAYLGIQNVLSYPIGMERDTHSSLSGDQKIIGRDDEMNNLKKNLLAED 201
           IS+E+ KF         ++ +              +  + K+ GRD E + + K +L  +
Sbjct: 130 ISEERRKFHF-------LEKITERQAAAATRETGFVLTEPKVYGRDKEEDEIVK-ILINN 189

Query: 202 DKVKANVSFIAIVGMGGIGKTTLAKSLYNDKQVSDGFSSRIWIWVSNQFDTKTILKKIIE 261
             V   +    I+GMGG+GKTTLA+ ++ND++V+  F+ +IW+ VS+ FD K ++K II 
Sbjct: 190 VNVAEELPVFPIIGMGGLGKTTLAQMIFNDERVTKHFNPKIWVCVSDDFDEKRLIKTIIG 249

Query: 262 SATEKKPKVEEMEPLKTKLEEVIGGKKYLLVMDDVWNENENEWENLKNLLMLGARGSKVL 321
           +     P VE++   + KL+E++ GK+YLLV+DDVWN++  +W  L+ +L +GARG+ +L
Sbjct: 250 NIERSSPHVEDLASFQKKLQELLNGKRYLLVLDDVWNDDLEKWAKLRAVLTVGARGASIL 309

Query: 322 ITKRDSKAVPGVETIP---LKDLTEDFSWLLFKEVAFKESDLESINQNLIKMGKEISKRC 381
            T R  K    + T+    L +L+   S LLF + AF +   +  N NL+ +GKEI K+C
Sbjct: 310 ATTRLEKVGSIMGTLQPYHLSNLSPHDSLLLFMQRAFGQQ--KEANPNLVAIGKEIVKKC 369

Query: 382 GGIPLVIRHIGRLLYGKTSAEDWEFIKENELLNVTREKNNNDGHVISTLKLSYNHLSPNL 441
           GG+PL  + +G LL  K    +WE +++NE+ ++ +++++    ++  L+LSY+HL  +L
Sbjct: 370 GGVPLAAKTLGGLLRFKREESEWEHVRDNEIWSLPQDESS----ILPALRLSYHHLPLDL 429

Query: 442 KQCFSYSSLFPKGYKIRMNELIRQWIAQGFIESSNGGKSVENIGKEYLDELCWRFFYEIS 501
           +QCF+Y ++FPK  K+    LI  W+A GF+  S G   +E++G E  +EL  R F++  
Sbjct: 430 RQCFAYCAVFPKDTKMIKENLITLWMAHGFL-LSKGNLELEDVGNEVWNELYLRSFFQEI 489

Query: 502 IEDVPFEEVGMHDLMCDLAREVAGQKLYIRGYPESGYVVSEQTRHISFEYEPRSWIDDVS 561
                     +HDL+ DLA  +           E      + T  I F     S+     
Sbjct: 490 EAKSGNTYFKIHDLIHDLATSLFSASASCGNIREINVKDYKHTVSIGFAAVVSSY----- 549

Query: 562 KLQQAKGLRTFLLFTKNPFFTRNPIEKVLLDRLFSHFPRLRVLQIPNVSKSIKKLRHLRY 621
                           +P   +  +   +L+  +S     ++ Q+P+    +  LR+L  
Sbjct: 550 ----------------SPSLLKKFVSLRVLNLSYS-----KLEQLPSSIGDLLHLRYLDL 609

Query: 622 LELGEDAKSVPNSITKLQNLQTLDLTKCYDLKELPRDINNFVNLRHLLCDSRLMNMLQGT 681
                + +S+P  + KLQNLQTLD+  CY L  LP+  +   +LRHL+ D   +      
Sbjct: 610 --SCNNFRSLPERLCKLQNLQTLDVHNCYSLNCLPKQTSKLSSLRHLVVDGCPLTSTPPR 669

Query: 682 MEKLTSLQTLSSFLFDCKRFDKVKEFSERSYFIEFDLKIKGLEQLRFSPSDVKSVNLKNK 741
           +  LT L+TL  F+   K+  ++ E   ++  +   + I  LE+++ + +D ++      
Sbjct: 670 IGLLTCLKTLGFFIVGSKKGYQLGEL--KNLNLCGSISITHLERVK-NDTDAEANLSAKA 729

Query: 742 KVPLLRLKWKFENGNEYEGDADDIVLEGLEPHPYVNLLQIEGYCGVGLPNWVSTSILLR- 801
            +  L + W  +  N YE   +  VLE L+PHP +  L+I  + G   P+W++ S+L + 
Sbjct: 730 NLQSLSMSWDNDGPNRYE-SKEVKVLEALKPHPNLKYLEIIAFGGFRFPSWINHSVLEKV 789

Query: 802 -GIRIGNCDR-LHLNQLSHLHALEILNLE-GLKSVMSISE--------WIGTLTSLVSLE 861
             +RI +C   L L     L  LE L L+ G   V  + E           +  SL  L 
Sbjct: 790 ISVRIKSCKNCLCLPPFGELPCLENLELQNGSAEVEYVEEDDVHSRFSTRRSFPSLKKLR 822

Query: 862 IEECPKLKSLPKE--MQQLKSLVQLNIIKCP 876
           I     LK L KE   ++   L ++ I+ CP
Sbjct: 850 IWFFRSLKGLMKEEGEEKFPMLEEMAILYCP 822

HSP 2 Score: 66.2 bits (160), Expect = 2.0e-09
Identity = 37/88 (42.05%), Postives = 55/88 (62.50%), Query Frame = 1

Query: 812 LSHLHALEILNLEGLKSVMSISEW-IGTLTSLVSLEIEECPKLKSLPKEMQQLKSLVQLN 871
           L+ L+AL+ L +E   S+ S  E  +  LTSL  L ++ C  LK LP+ +Q L +L  L 
Sbjct: 898 LTSLNALKRLQIESCDSLESFPEQGLEGLTSLTQLFVKYCKMLKCLPEGLQHLTALTNLG 957

Query: 872 IIKCPQLGERC-KEGGEDWPNISHIPDV 898
           +  CP++ +RC KE GEDW  I+HIP++
Sbjct: 958 VSGCPEVEKRCDKEIGEDWHKIAHIPNL 985

BLAST of Cucsa.128130 vs. Swiss-Prot
Match: R13L1_ARATH (Putative disease resistance RPP13-like protein 1 OS=Arabidopsis thaliana GN=RPPL1 PE=3 SV=1)

HSP 1 Score: 345.1 bits (884), Expect = 2.3e-93
Identity = 241/823 (29.28%), Postives = 433/823 (52.61%), Query Frame = 1

Query: 39  LNDDL-NKLRSNVSSIQSVLRDAEQRQIKGNDHSLTDWLEKLGDVFYDVEDVLDEISTEA 98
           LN++L  +L + + +I +VL DAE++QI   +  +  W+ +L DV Y  ED LD+I+TEA
Sbjct: 34  LNENLLERLSTALLTITAVLIDAEEKQI--TNPVVEKWVNELRDVVYHAEDALDDIATEA 93

Query: 99  LRREVMTRGKNAKQVRIFFSNSNQLAF----NYRMACQVKKINERLDVISQEKDKFQLNG 158
           LR  +     ++ ++R      +   F    +  +  +++K+  RL+ ++ +++      
Sbjct: 94  LRLNIGAESSSSNRLRQLRGRMSLGDFLDGNSEHLETRLEKVTIRLERLASQRN------ 153

Query: 159 IAYLGIQNVLSYPIGMERDTHSSLSGDQKIIGRDDEMNNLKKNLLAEDDKVKANVSFIAI 218
              LG++  L+  I  +R   +SL  + ++ GRDD+ + + + L+ E+ K    ++ +AI
Sbjct: 154 --ILGLKE-LTAMIPKQRLPTTSLVDESEVFGRDDDKDEIMRFLIPENGKDNG-ITVVAI 213

Query: 219 VGMGGIGKTTLAKSLYNDKQVSDGFSSRIWIWVSNQFDTKTILKKIIESATEKKPKVEEM 278
           VG+GG+GKTTL++ LYND+ V   F +++W  VS +FD   I KK+ ES T +  +  ++
Sbjct: 214 VGIGGVGKTTLSQLLYNDQHVRSYFGTKVWAHVSEEFDVFKITKKVYESVTSRPCEFTDL 273

Query: 279 EPLKTKLEEVIGGK--KYLLVMDDVWNENENEWENLKNLLMLGARGSKVLITKRDSKAVP 338
           + L+ KL+E + G    +LLV+DD+WNEN  +W+ L+   +  A+GS++L+T R  +   
Sbjct: 274 DVLQVKLKERLTGTGLPFLLVLDDLWNENFADWDLLRQPFIHAAQGSQILVTTRSQRVAS 333

Query: 339 ---GVETIPLKDLTEDFSWLLFKEVAFKESDLESINQNLIKMGKEISKRCGGIPLVIRHI 398
               V    L+ L++   W LF +  F   +   +N+ +  + + I  +C G+PL ++ +
Sbjct: 334 IMCAVHVHNLQPLSDGDCWSLFMKTVFGNQE-PCLNREIGDLAERIVHKCRGLPLAVKTL 393

Query: 399 GRLLYGKTSAEDWEFIKENELLNVTREKNNNDGHVISTLKLSYNHLSPNLKQCFSYSSLF 458
           G +L  +    +WE +  + + ++  +K+N    ++  L++SY +L  +LK+CF+Y S+F
Sbjct: 394 GGVLRFEGKVIEWERVLSSRIWDLPADKSN----LLPVLRVSYYYLPAHLKRCFAYCSIF 453

Query: 459 PKGYKIRMNELIRQWIAQGFIESSNGGKSVENIGKEYLDELCWRFFYEISIEDVPFEEVG 518
           PKG+    ++++  W+A+GF++ +   K++E +G EY  EL  R     S+         
Sbjct: 454 PKGHAFEKDKVVLLWMAEGFLQQTRSSKNLEELGNEYFSELESR-----SLLQKTKTRYI 513

Query: 519 MHDLMCDLAREVAGQKLYIRGYPESG--YVVSEQTRHISFEYEPRSWIDDVSKLQQAKGL 578
           MHD + +LA+  +G+        E G    VSE+TR++S+  +  +   +   L++ K L
Sbjct: 514 MHDFINELAQFASGE---FSSKFEDGCKLQVSERTRYLSYLRDNYAEPMEFEALREVKFL 573

Query: 579 RTFLLFTKNPFFTRNPIEKVLLDRLFSHFPRLRVLQIPNVSKS------IKKLRHLRYLE 638
           RTFL  +         +++++ ++L     RLRVL + +   +       K + H R+L+
Sbjct: 574 RTFLPLSLTNSSRSCCLDQMVSEKLLPTLTRLRVLSLSHYKIARLPPDFFKNISHARFLD 633

Query: 639 LGE-DAKSVPNSITKLQNLQTLDLTKCYDLKELPRDINNFVNLRHLLCDSRLMNMLQGTM 698
           L   + + +P S+  + NLQTL L+ C  LKELP DI+N +NLR+L      +  +    
Sbjct: 634 LSRTELEKLPKSLCYMYNLQTLLLSYCSSLKELPTDISNLINLRYLDLIGTKLRQMPRRF 693

Query: 699 EKLTSLQTLSSFLFDCKRFDKVKEFSERSYFIEFDLKIKGLEQLRFSPSDVKSVNLKNKK 758
            +L SLQTL++F        ++ E     + +   LKI  L+++    +D    NL +KK
Sbjct: 694 GRLKSLQTLTTFFVSASDGSRISELG-GLHDLHGKLKIVELQRV-VDVADAAEANLNSKK 753

Query: 759 -VPLLRLKWKF------ENGNEYEGDADDIVLEGLEPHPYVNLLQIEGYCGVGLPNWVST 818
            +  +   W+        N N +    +  V E L PH ++  L IE Y G   P+W+S 
Sbjct: 754 HLREIDFVWRTGSSSSENNTNPHRTQNEAEVFEKLRPHRHIEKLAIERYKGRRFPDWLSD 813

Query: 819 SILLR--GIRIGNCDRL----HLNQLSHLHALEILNLEGLKSV 830
               R   IR+  C        L QL  L  L I  + GL+S+
Sbjct: 814 PSFSRIVCIRLRECQYCTSLPSLGQLPCLKELHISGMVGLQSI 829

BLAST of Cucsa.128130 vs. TrEMBL
Match: A0A0A0K9P2_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_7G425890 PE=3 SV=1)

HSP 1 Score: 1077.8 bits (2786), Expect = 0.0e+00
Identity = 531/531 (100.00%), Postives = 531/531 (100.00%), Query Frame = 1

Query: 370 MGKEISKRCGGIPLVIRHIGRLLYGKTSAEDWEFIKENELLNVTREKNNNDGHVISTLKL 429
           MGKEISKRCGGIPLVIRHIGRLLYGKTSAEDWEFIKENELLNVTREKNNNDGHVISTLKL
Sbjct: 1   MGKEISKRCGGIPLVIRHIGRLLYGKTSAEDWEFIKENELLNVTREKNNNDGHVISTLKL 60

Query: 430 SYNHLSPNLKQCFSYSSLFPKGYKIRMNELIRQWIAQGFIESSNGGKSVENIGKEYLDEL 489
           SYNHLSPNLKQCFSYSSLFPKGYKIRMNELIRQWIAQGFIESSNGGKSVENIGKEYLDEL
Sbjct: 61  SYNHLSPNLKQCFSYSSLFPKGYKIRMNELIRQWIAQGFIESSNGGKSVENIGKEYLDEL 120

Query: 490 CWRFFYEISIEDVPFEEVGMHDLMCDLAREVAGQKLYIRGYPESGYVVSEQTRHISFEYE 549
           CWRFFYEISIEDVPFEEVGMHDLMCDLAREVAGQKLYIRGYPESGYVVSEQTRHISFEYE
Sbjct: 121 CWRFFYEISIEDVPFEEVGMHDLMCDLAREVAGQKLYIRGYPESGYVVSEQTRHISFEYE 180

Query: 550 PRSWIDDVSKLQQAKGLRTFLLFTKNPFFTRNPIEKVLLDRLFSHFPRLRVLQIPNVSKS 609
           PRSWIDDVSKLQQAKGLRTFLLFTKNPFFTRNPIEKVLLDRLFSHFPRLRVLQIPNVSKS
Sbjct: 181 PRSWIDDVSKLQQAKGLRTFLLFTKNPFFTRNPIEKVLLDRLFSHFPRLRVLQIPNVSKS 240

Query: 610 IKKLRHLRYLELGEDAKSVPNSITKLQNLQTLDLTKCYDLKELPRDINNFVNLRHLLCDS 669
           IKKLRHLRYLELGEDAKSVPNSITKLQNLQTLDLTKCYDLKELPRDINNFVNLRHLLCDS
Sbjct: 241 IKKLRHLRYLELGEDAKSVPNSITKLQNLQTLDLTKCYDLKELPRDINNFVNLRHLLCDS 300

Query: 670 RLMNMLQGTMEKLTSLQTLSSFLFDCKRFDKVKEFSERSYFIEFDLKIKGLEQLRFSPSD 729
           RLMNMLQGTMEKLTSLQTLSSFLFDCKRFDKVKEFSERSYFIEFDLKIKGLEQLRFSPSD
Sbjct: 301 RLMNMLQGTMEKLTSLQTLSSFLFDCKRFDKVKEFSERSYFIEFDLKIKGLEQLRFSPSD 360

Query: 730 VKSVNLKNKKVPLLRLKWKFENGNEYEGDADDIVLEGLEPHPYVNLLQIEGYCGVGLPNW 789
           VKSVNLKNKKVPLLRLKWKFENGNEYEGDADDIVLEGLEPHPYVNLLQIEGYCGVGLPNW
Sbjct: 361 VKSVNLKNKKVPLLRLKWKFENGNEYEGDADDIVLEGLEPHPYVNLLQIEGYCGVGLPNW 420

Query: 790 VSTSILLRGIRIGNCDRLHLNQLSHLHALEILNLEGLKSVMSISEWIGTLTSLVSLEIEE 849
           VSTSILLRGIRIGNCDRLHLNQLSHLHALEILNLEGLKSVMSISEWIGTLTSLVSLEIEE
Sbjct: 421 VSTSILLRGIRIGNCDRLHLNQLSHLHALEILNLEGLKSVMSISEWIGTLTSLVSLEIEE 480

Query: 850 CPKLKSLPKEMQQLKSLVQLNIIKCPQLGERCKEGGEDWPNISHIPDVLID 901
           CPKLKSLPKEMQQLKSLVQLNIIKCPQLGERCKEGGEDWPNISHIPDVLID
Sbjct: 481 CPKLKSLPKEMQQLKSLVQLNIIKCPQLGERCKEGGEDWPNISHIPDVLID 531

BLAST of Cucsa.128130 vs. TrEMBL
Match: A0A061EA34_THECC (LRR and NB-ARC domains-containing disease resistance protein, putative OS=Theobroma cacao GN=TCM_011164 PE=3 SV=1)

HSP 1 Score: 511.9 bits (1317), Expect = 1.6e-141
Identity = 331/852 (38.85%), Postives = 494/852 (57.98%), Query Frame = 1

Query: 24  KLSSHALECLGMVCGLNDDLNKLRSNVSSIQSVLRDAEQRQIKGNDHSLTDWLEKLGDVF 83
           KL +  L+ +G++ G+ ++L KL++ VS+I++VL DAE++  K   H + DWL KL D  
Sbjct: 23  KLGNLTLQEMGLIWGVKEELEKLKNTVSTIKAVLLDAEEQHAKS--HEVIDWLGKLKDAV 82

Query: 84  YDVEDVLDEISTEALRREVMTRGKNAKQVRIFFSNSNQLAFNYRMACQVKKINERLDVIS 143
           YD +D+LD+ ST  L+R+VM +GK  KQV   FS +NQ+A+N ++  Q+K I +RLD I+
Sbjct: 83  YDADDLLDDFSTHVLQRQVMMQGKRGKQVSFLFSKANQVAYNLKIGHQIKAIRQRLDAIA 142

Query: 144 QEKDKFQLNGIAYLGIQNVLSYPI-GMERDTHSSLSGDQKIIGRDDEMNNLKKNLLAEDD 203
            +K K+          ++++S P+  +ER    S    + ++GR+ +   + K LL  D 
Sbjct: 143 ADKTKYHFTD------RSLVSIPVVKVERKQTHSFVRKEGVVGREGDKEAIMKRLL--DS 202

Query: 204 KVKANVSFIAIVGMGGIGKTTLAKSLYNDKQVSDGFSSRIWIWVSNQFDTKTILKKIIES 263
            V  NVS I IVG+GG GKTT+A+ +YND+++   F  RIW+ VS+ FD K I +K++ES
Sbjct: 203 DVADNVSVIPIVGIGGQGKTTVAELVYNDEKIVKHFELRIWVCVSDVFDVKLIAQKMLES 262

Query: 264 ATEKKPKVEEMEPLKTKLEEVIGGKKYLLVMDDVWNENENEWENLKNLLMLGARGSKVLI 323
           AT  K +  EM+ L+T L + I G+KYLL++DDVWN+N   W NL++LLM GARGSK+++
Sbjct: 263 ATNAKFENLEMDSLQTHLRKRIDGRKYLLILDDVWNDNRERWRNLRDLLMNGARGSKIIV 322

Query: 324 TKRDS--KAVPG-VETIPLKDLTEDFSWLLFKEVAFKESDLESINQNLIKMGKEISKRCG 383
           T R     ++ G  E   L+ L ED SW L +++AFKE   E  N  L+ +GK+I KRC 
Sbjct: 323 TTRAQVVASITGTTEPYLLEGLPEDMSWSLLEKMAFKEG-REPNNSRLVAIGKDIVKRCA 382

Query: 384 GIPLVIRHIGRLLYGKTSAEDWEFIKENELLNVTREKNNNDGHVISTLKLSYNHLSPNLK 443
           G PL IR IG +LY K +  +W  +KE +LL +T++ +     V+  LKLSY  L   LK
Sbjct: 383 GNPLAIRTIGGVLYTKDTETEWLSLKEGQLLMITQKDD-----VLPILKLSYEQLPSYLK 442

Query: 444 QCFSYSSLFPKGYKIRMNELIRQWIAQGFIESSNGGKSVENIGKEYLDELCWRFFYEISI 503
           QCF+Y SLFP+ Y+I    LI  W+A+GFI+S  G + +E +G +Y  +L  R F+    
Sbjct: 443 QCFAYCSLFPRDYEINKQMLITLWMAEGFIQSLQGMQRLEELGDQYFMDLLRRSFF---- 502

Query: 504 EDVPFEEVG------MHDLMCDLAREVAGQKLYIRGYPESGYV------VSEQTRHISFE 563
           +DV ++E G      MHDLM DLA+ VAG         +S  V      +SE+ RH+SF 
Sbjct: 503 QDVEYDEWGNVISCKMHDLMHDLAQLVAGS--------DSSMVDLDCKNISERIRHVSFN 562

Query: 564 YEPRSWIDDVSKLQQAKGLRTFLLFTKNPFFTRNPIEKVLLDR-----LFSHFPRLRVLQ 623
            E  S     + L +A  +RTFLL  +       P+ +V+LD+     + S F  LR+L 
Sbjct: 563 AELDSSWKIPTPLLKATKIRTFLLPVQ-------PVHRVILDKVDHETVISSFRLLRLLD 622

Query: 624 IPN-----VSKSIKKLRHLRYLEL--GEDAKSVPNSITKLQNLQTLDLTKCYDLKELPRD 683
           + N     +  SI  L+HLRYL+L   E  + +P+SIT+L NLQTL L  C  L+ELPR 
Sbjct: 623 LHNTGIDILPSSIGTLKHLRYLDLSKNEVIRRLPSSITELLNLQTLKLCSCKRLEELPRK 682

Query: 684 INNFVNLRHLLCD--SRLMNMLQGTMEKLTSLQTLSSFLFDCKRFDK----VKEFSERSY 743
           + N  +LRHL     + L  M  G + +LTSLQTL+ F+     F +    ++E  + + 
Sbjct: 683 LRNMTSLRHLETGQCTGLTRMPSG-LGQLTSLQTLTRFVVGMDSFKRPSGGLRELKDLN- 742

Query: 744 FIEFDLKIKGLEQLRFSPSDVKSVNLKNKK-VPLLRLKWKFENGNEYEGDADDIVLEGLE 803
            +  ++ I  LE L+   S+ K  N K K+ + +L L+W  E  +    + D+ +LEGL+
Sbjct: 743 DLRGEVMIAKLENLKNVVSECKEANSKGKQHLEVLTLEWSREVNDHTTFEEDEEILEGLQ 802

Query: 804 PHPYVNLLQIEGYCGVGLPNWVSTSIL-----LRGIRIGNCDR-LHLNQLSHLHALEILN 835
           PH  +    I GY     P W+ + +      L  I +  C+R LHL   SHL  L +L 
Sbjct: 803 PHSNLQEFHIYGYRAGSFPKWMLSDMSLVLPNLLEITLWRCNRCLHLPLFSHLPKLRVLR 837

BLAST of Cucsa.128130 vs. TrEMBL
Match: A0A061EA34_THECC (LRR and NB-ARC domains-containing disease resistance protein, putative OS=Theobroma cacao GN=TCM_011164 PE=3 SV=1)

HSP 1 Score: 72.4 bits (176), Expect = 3.2e-09
Identity = 39/108 (36.11%), Postives = 64/108 (59.26%), Query Frame = 1

Query: 796  LRGIRIGNCDRLHL--NQLSHLHALEILNLEGLKSVMSISEWIGTLTSLVSLEIEECPKL 855
            LR ++IG+  +L+   + L H+  L+ L +    ++ S+ EWI  LT L   EI +CP+L
Sbjct: 1081 LRTLKIGDMPKLNSLPDGLQHVTTLKYLQISSCSNLKSLPEWICNLTVLQRFEIFDCPQL 1140

Query: 856  KSLPKEMQQLKSLVQLNIIKCPQLGERCK-EGGEDWPNISHIPDVLID 901
             SL + +  L +L  L I  CP L + C+ +  ++WP I+HIP++ ID
Sbjct: 1141 VSLQQALCSLNALQYLEISGCPLLMDTCQNKTSKNWPRIAHIPEIYID 1188

HSP 2 Score: 49.7 bits (117), Expect = 2.2e-02
Identity = 38/138 (27.54%), Postives = 62/138 (44.93%), Query Frame = 1

Query: 796  LRGIRIGNCDRLHLN--------QLSHLHALEILNLEGLKSVMSISEWIGTLTSLVSLEI 855
            L  + I  C  L L+        +L  L +L  L +  +  + S+ + +  +T+L  L+I
Sbjct: 1051 LEELEIKECRELDLSKDVEENVMELRFLRSLRTLKIGDMPKLNSLPDGLQHVTTLKYLQI 1110

Query: 856  EECPKLKSLPK--------------------EMQQ----LKSLVQLNIIKCPQLGERCK- 901
              C  LKSLP+                     +QQ    L +L  L I  CP L + C+ 
Sbjct: 1111 SSCSNLKSLPEWICNLTVLQRFEIFDCPQLVSLQQALCSLNALQYLEISGCPLLMDTCQN 1170

HSP 3 Score: 39.7 bits (91), Expect = 2.3e+01
Identity = 31/90 (34.44%), Postives = 39/90 (43.33%), Query Frame = 1

Query: 796  LRGIRIGNCDRL--------HLNQLSHLHALEILNLEGLKSVMSISEWIGTLTSLVSLEI 855
            LR   I  CD L        HL  L  L   E   L+  K V      +  L SL +L+I
Sbjct: 1027 LRFFFISGCDMLKSLSKGFRHLTALEELEIKECRELDLSKDVEENVMELRFLRSLRTLKI 1086

Query: 856  EECPKLKSLPKEMQQLKSLVQLNIIKCPQL 878
             + PKL SLP  +Q + +L  L I  C  L
Sbjct: 1087 GDMPKLNSLPDGLQHVTTLKYLQISSCSNL 1116

HSP 4 Score: 38.5 bits (88), Expect = 5.1e+01
Identity = 35/116 (30.17%), Postives = 57/116 (49.14%), Query Frame = 1

Query: 588  LDRLFSHFPRLRVLQIP-----NVSKSIKK-------LRHLRYLELGEDAK--SVPNSIT 647
            L + F H   L  L+I      ++SK +++       LR LR L++G+  K  S+P+ + 
Sbjct: 1041 LSKGFRHLTALEELEIKECRELDLSKDVEENVMELRFLRSLRTLKIGDMPKLNSLPDGLQ 1100

Query: 648  KLQNLQTLDLTKCYDLKELPRDINNFVNL-RHLLCDSRLMNMLQGTMEKLTSLQTL 689
             +  L+ L ++ C +LK LP  I N   L R  + D   +  LQ  +  L +LQ L
Sbjct: 1101 HVTTLKYLQISSCSNLKSLPEWICNLTVLQRFEIFDCPQLVSLQQALCSLNALQYL 1156

HSP 5 Score: 31.2 bits (69), Expect = 8.1e+03
Identity = 19/69 (27.54%), Postives = 37/69 (53.62%), Query Frame = 1

Query: 809 LNQLSHLHALEILNLEGLKSVMSISEWIGTLTSLVSLEIEECPKLKSLPKEMQQLKSLVQ 868
           +  L HL  L++   E ++ + S    I  L +L +L++  C +L+ LP++++ + SL  
Sbjct: 600 IGTLKHLRYLDLSKNEVIRRLPSS---ITELLNLQTLKLCSCKRLEELPRKLRNMTSLRH 659

Query: 869 LNIIKCPQL 878
           L   +C  L
Sbjct: 660 LETGQCTGL 665

HSP 6 Score: 503.1 bits (1294), Expect = 7.3e-139
Identity = 335/890 (37.64%), Postives = 509/890 (57.19%), Query Frame = 1

Query: 22  IKKLSSHALECLGMVCGLNDDLNKLRSNVSSIQSVLRDAEQRQIKGNDHSLTDWLEKLGD 81
           I  L+S AL    +  G+ D+L+KL++ VS+IQ+V+  AE++      H +  W++ L +
Sbjct: 14  ISNLASPALRGAKLWWGVTDELDKLKTAVSTIQAVILGAEKQY--SQSHEVKAWVDSLKE 73

Query: 82  VFYDVEDVLDEISTEALRREVMTRGKNAKQVRIFFSNSNQLAFNYRMACQVKKINERLDV 141
            FYD +D+LDE+ST+ L+ +++T     K+VR+FFS+SNQ  ++ +MA +VKKI  +L  
Sbjct: 74  AFYDADDLLDELSTKELQEQLLTSKNLGKKVRLFFSSSNQFLYSIKMAHKVKKIRSKLAE 133

Query: 142 ISQEKDKFQLNGIAYLGIQNVLSYPIGMERD-THSSLSGDQKIIGRDDEMNNLKKNLLAE 201
           I++ + KF LN        +  +Y    ERD +HS L G   +IGR  +   + K LL+ 
Sbjct: 134 IAENR-KFLLNDEHRERKDDRTNYE---ERDQSHSFLPG--LVIGRKRDKKAMMKFLLSS 193

Query: 202 DDKVKANVSFIAIVGMGGIGKTTLAKSLYNDKQVSDGFSSRIWIWVSNQFDTKTILKKII 261
           +   + NVS I+IVG+GG+GKTTLA+ ++ND+ V   F  +IW+ +S+ FD KTIL+KI+
Sbjct: 194 N--CEENVSVISIVGIGGLGKTTLAQLVFNDETVESQFDLKIWVCISDNFDVKTILEKIL 253

Query: 262 ESATEKKPKVE-EMEPLKTKLEEVIGGKKYLLVMDDVWNENENEWENLKNLLMLGARGSK 321
           ES T +KP    EM  LK  L E I GKKYLLV+DDVW+  +  W  LK+LL + A GSK
Sbjct: 254 ESITREKPARNCEMNTLKNLLHEKIDGKKYLLVLDDVWHYEK--WFLLKDLLDVSASGSK 313

Query: 322 VLITKR---DSKAVPGVETIPLKDLTEDFSWLLFKEVAFKESDLESINQNLIKMGKEISK 381
           +++T R    ++ +       L+ L++  SW LF ++AF++  +ES    L+  GKEI  
Sbjct: 314 IIVTTRLKIVAEKIISDSIYELQGLSKQDSWSLFVQMAFRQGKVESQEHELV--GKEIVA 373

Query: 382 RCGGIPLVIRHIGRLLYGKTSAEDWEFIKENELLNVTREKNNNDGHVISTLKLSYNHLSP 441
            C G+PL IR IGRLLY K +  +W   K+ EL+N+ +E    D  ++ TLKLSYNHL  
Sbjct: 374 NCAGVPLAIRAIGRLLYFKNTLSEWLSFKDKELVNINKE----DASILPTLKLSYNHLPS 433

Query: 442 NLKQCFSYSSLFPKGYKIRMNELIRQWIAQGFIESSNGGKSVENIGKEYLDELCWRFFYE 501
           +LK CF+Y SLFPK  +I +  L+  W+AQG+IE S   +++E+IG +Y + L WR F+ 
Sbjct: 434 HLKHCFAYCSLFPKDTRINVIHLVHHWVAQGYIEVSGSKQNLEDIGLQYFENLLWRSFF- 493

Query: 502 ISIEDVPFEEVG------MHDLMCDLAREVAGQKLYIRGYPESGYVVSEQTRHISFEYEP 561
              ++V  + +G      MHDLM DL+ EVA +++ +         V E+T H+S   E 
Sbjct: 494 ---QEVEKDSLGNIITCKMHDLMSDLSVEVAREEITLLNSEAEFVNVKERTLHLSINPEV 553

Query: 562 RSWIDDVSKLQQAKGLRTFLLFTKNPFFTRNPIEKVLLDRLFSHFPRLRVLQIPNVS--- 621
            S       L  AK +RTFLL  K+     N I +  LD +FS F R+RVL + N+S   
Sbjct: 554 LSGRQLACILPNAKKVRTFLLLNKS---MANEIREGQLDIIFSDFRRIRVLDLSNLSIER 613

Query: 622 --KSIKKLRHLRYLELGED--AKSVPNSITKLQNLQTLDLTKCYDLKELPRDINNFVNLR 681
              SI K++H+RYL L ++   K +PNSIT LQNLQTL L +C  LK+LP+ +   VNLR
Sbjct: 614 VPPSIDKMKHIRYLNLSQNKGIKVLPNSITNLQNLQTLVLIECGRLKQLPKHMKKLVNLR 673

Query: 682 HL-LCDSRLMNMLQGTMEKLTSLQTLSSFLF--DCKRFDKVKEFSERSYF--IEFDLKIK 741
           HL + D   +  +   + +LTSLQ LS F+   D +   +   F E      +   L+I 
Sbjct: 674 HLYIRDCPSLTHMPYGLGQLTSLQNLSMFVVAKDNRISKQSGGFDELCNLNNLRGSLRIC 733

Query: 742 GLEQLRFSPSDVKSVNLKNKK-VPLLRLKWKF-----ENGNEYEGDADDIVLEGLEPHPY 801
            L+ ++ + ++ ++  L++K+ +  L L WK      ++ +    D  +I LE L+PH  
Sbjct: 734 NLQYVKNATAEFEAAKLEDKQHLQNLHLAWKLGAPYDKSESNTNDDNGEISLEELQPHRN 793

Query: 802 VNLLQIEGYCGVGLPNWVSTSILLRGIRIGNCDRLH----LNQLSHLHALEILNLEGLKS 861
           +  L I G   V  P W+ST   L  ++I NC        L+    L  L I NL  L+ 
Sbjct: 794 LKWLVISGCGKVKFPGWISTLCCLVDLQINNCTNCKCFPPLDHFPSLKRLSIQNLTELEY 853

Query: 862 VMSISEWIGTLTSLVSLEIEECPKLKSLPK-EMQQLKSLVQLNIIKCPQL 878
           + + +   G  +++  L +  CP LK   + ++ Q   L  L+I+ CP L
Sbjct: 854 IENGNRMSG--STMEELWLINCPNLKGWRRPQLLQFHCLTYLDIMSCPNL 876

BLAST of Cucsa.128130 vs. TrEMBL
Match: A0A067KN28_JATCU (Uncharacterized protein OS=Jatropha curcas GN=JCGZ_07835 PE=3 SV=1)

HSP 1 Score: 73.6 bits (179), Expect = 1.4e-09
Identity = 44/124 (35.48%), Postives = 65/124 (52.42%), Query Frame = 1

Query: 786  LPNWVSTSILLRGIRIGNCDRLHL--------NQLSHLHALEILNLEGLKSVMSISEWIG 845
            LP  +     L+ + I  C++L           Q   L +L+ +  + +  ++S+ + + 
Sbjct: 971  LPCAIQHLTSLKALLICGCEKLDFLADKNENGMQWQCLRSLKRVQFKNMAKLVSLPKGLQ 1030

Query: 846  TLTSLVSLEIEECPKLKSLPKEMQQLKSLVQLNIIKCPQLGERC-KEGGEDWPNISHIPD 901
             +T+L  L IE CP L SLPK M  L  L  L + KCP L ERC K  G DWP I+HIP+
Sbjct: 1031 HVTTLRKLGIESCPNLMSLPKWMTNLTRLQYLELDKCPNLSERCSKNMGADWPMIAHIPN 1090

HSP 2 Score: 502.3 bits (1292), Expect = 1.2e-138
Identity = 343/870 (39.43%), Postives = 498/870 (57.24%), Query Frame = 1

Query: 22  IKKLSSHALECLGMVCGLNDDLNKLRSNVSSIQSVLRDAEQRQIKGNDHSLTDWLEKLGD 81
           I KLSS  L  +G+   + DDL+ L+S VS+I++VL DAE+R +    H + DWLEKL D
Sbjct: 14  ITKLSSFTLSQIGLWWNVKDDLDDLKSTVSTIKAVLLDAEERSV--TSHHVKDWLEKLKD 73

Query: 82  VFYDVEDVLDEISTEALRREVMTRGKNAKQVRIFFSNSNQLAFNYRMACQVKKINERLDV 141
           V YD +D+LD+ STEA R++++   K  K+VR+FFS+SNQ A++ +M  Q+K I  RL +
Sbjct: 74  VLYDADDLLDDFSTEAFRKDLLGGNKLTKEVRLFFSSSNQFAYSLKMGRQIKAIKARLTL 133

Query: 142 ISQEKDKFQLNGIAYLGIQNVLSYPIGMERDTHSSLSGDQKIIGRDDEMNNLKKNLLAED 201
           I  E   F L                      H + S    IIGRDD+   L K +L  +
Sbjct: 134 IGSEAKMFNLR---------------------HQTHSFKDNIIGRDDDKAALLKLML--E 193

Query: 202 DKVKANVSFIAIVGMGGIGKTTLAKSLYNDKQVSDGFSSRIWIWVSNQFDTKTILKKIIE 261
            + + NV  I IVG+GG+GKT LA+ +YND+ V + F   +++ VS+ FD K I++ II+
Sbjct: 194 FESEENVYIIPIVGLGGLGKTALAQFVYNDEMVKNHFELMMFVCVSDVFDVKIIVENIIK 253

Query: 262 SATEKKPKVE-EMEPLKTKLEEVIGGKKYLLVMDDVWNENENEWENLKNLLMLGARGSKV 321
           SAT + P  + EM+ L+++L   IGGKKYLLV+DD+WNE   EW +LK LL+ GA+GS++
Sbjct: 254 SATGQAPNQKLEMDQLQSQLRGKIGGKKYLLVLDDIWNEEWEEWVSLKELLVGGAKGSRI 313

Query: 322 LITKRD---SKAVPGVETIPLKDLTEDFSWLLFKEVAFKESDLESINQNLIKMGKEISKR 381
           ++T R    +K     +   LK L++D +W LFKE+AF++    SIN   +K+GK+I +R
Sbjct: 314 IVTTRSLRVAKITSKCQPYVLKGLSDDDAWSLFKEIAFEQ---RSINPTFVKIGKQILER 373

Query: 382 CGGIPLVIRHIGRLLYGKTSAEDWEFIKENELLNVTREKNNNDGHVISTLKLSYNHLSPN 441
           CGG+PLVIR I   L  K + ++W   K+NEL  +++    N+G +++TLKLSY+HL  +
Sbjct: 374 CGGVPLVIRTIAGALSFKETEKEWSSFKDNELARISQ----NEGKILATLKLSYDHLPSH 433

Query: 442 LKQCFSYSSLFPKGYKIRMNELIRQWIAQGFIESSNGGKSVENIGKEYLDELCWRFFYEI 501
           LK CF+Y  L+PK +KI +  L++ WIAQGF++  N  +S+E IG  Y  +L  R F++ 
Sbjct: 434 LKHCFAYCRLYPKDHKIHVQTLVQFWIAQGFVKQLNPSQSLEEIGFVYFKDLNERGFFQE 493

Query: 502 SIEDVPFEEV--GMHDLMCDLAREVAGQKLYIRGYPESGYVVSEQTRHISFEYEPRSWID 561
             E   +E +   MHDLM DLA  VAG +  I    E    V E+ RHIS +        
Sbjct: 494 VGERYSWEGLTCKMHDLMHDLAESVAGTESSIVDSNEIASKVGEKCRHISID-------P 553

Query: 562 DVSKLQQAKGLRTFLLFTKNPFFTRNP-IEKVLLDRLFSHFPRLRVLQIPN-----VSKS 621
            +  L + K LRT L F   P + RN  +   + D + S++  LRVL++       + +S
Sbjct: 554 SLIVLFKGKKLRTMLHF---PNYERNQNMNDEIWDFIISNYRCLRVLELNGLDFKMIPRS 613

Query: 622 IKKLRHLRYLELGE--DAKSVPNSITKLQNLQTLDLTKCYDLKELPRDINNFVNLRHLLC 681
           I KL+HLRYL+L    + K +P SI K+QNLQ L L  C++L+ELP+ I   VNL HL C
Sbjct: 614 ICKLKHLRYLDLSGNFNIKILPKSICKIQNLQALKLNLCWELEELPKKIEKLVNLTHLGC 673

Query: 682 D--SRLMNMLQGTMEKLTSLQTLSSFLFDCKRFDKVKEFSERSYF--IEFDLKIKGLEQL 741
           +  S L +M +G + KLTSL+TLS F+ D        + SE S    +   L+I  L  +
Sbjct: 674 EDCSGLTHMPRG-IGKLTSLETLSKFVVDKDGSRDGADLSELSGLNNLTGRLEITNLGFV 733

Query: 742 RFSPSDVKSVNLKNKK-VPLLRLKWK------FENGNEYEGDADDIVLEGLEPHPYVNLL 801
           + +    K+ NLK K+ + LL L W        ++GN  + D DD  LE L PHP +  L
Sbjct: 734 KNAKEKFKAANLKEKQHLRLLVLVWNDYGDDDDDSGN--DDDDDDKSLEDLRPHPNLKEL 793

Query: 802 QIEGY-CGVGLPNWVSTSILLRGIRI---GNCDRL-HLNQLSHLHALEILNLEGLKSV-- 856
            I G+      P+W+S    L  IRI    N  ++    QL  L  L I++L  L+ +  
Sbjct: 794 FIGGWRSDAKFPSWLSLLTNLVVIRIWGPSNFKQIPSFAQLPCLQKLSIVDLTLLEYMDD 838

BLAST of Cucsa.128130 vs. TrEMBL
Match: A0A0D2SQD8_GOSRA (Uncharacterized protein OS=Gossypium raimondii GN=B456_007G365300 PE=3 SV=1)

HSP 1 Score: 76.6 bits (187), Expect = 1.7e-10
Identity = 60/175 (34.29%), Postives = 79/175 (45.14%), Query Frame = 1

Query: 757  GDADDIVLEGLEPHPYVNLLQIEGYCG----VGLPNWVSTSILLRGIRIGNCDRL----- 816
            GD  ++ LEG++     NL  + G       V LP W+   + L+ + I NC  L     
Sbjct: 956  GDCKEVDLEGMQWEALKNLSHL-GIINIPQLVSLPLWLQHLVQLKRLEIWNCSGLRSLFP 1015

Query: 817  ---HLNQLSHLHA---------------------LEILNLEGLKSVMSISEWIGTLTSLV 876
               HL  L  L                       L  L LE +     + EWI  LT+L 
Sbjct: 1016 VFQHLTFLEDLLVSKCKELELSAAGLQIFQDQTRLRSLALENISKCRHLPEWIQHLTNLQ 1075

Query: 877  SLEIEECPKLKSLPKEMQQLKSLVQLNIIKCPQLGERC-KEGGEDWPNISHIPDV 898
             L + + P L SLP EM+ L +L QLNI + PQL ERC K+ G DW  I+HIP V
Sbjct: 1076 ILSLVDWPNLTSLPDEMRCLTNLQQLNIHEVPQLEERCQKDIGADWYKIAHIPSV 1129

HSP 2 Score: 32.3 bits (72), Expect = 3.6e+03
Identity = 22/89 (24.72%), Postives = 45/89 (50.56%), Query Frame = 1

Query: 790 VSTSILLRGIRIGNCD-RLHLNQLSHLHALEILNLEGLKSVMSISEWIGTLTSLVSLEIE 849
           +S    LR + +   D ++    +  L  L  L+L G  ++  + + I  + +L +L++ 
Sbjct: 549 ISNYRCLRVLELNGLDFKMIPRSICKLKHLRYLDLSGNFNIKILPKSICKIQNLQALKLN 608

Query: 850 ECPKLKSLPKEMQQLKSLVQLNIIKCPQL 878
            C +L+ LPK++++L +L  L    C  L
Sbjct: 609 LCWELEELPKKIEKLVNLTHLGCEDCSGL 637

HSP 3 Score: 501.5 bits (1290), Expect = 2.1e-138
Identity = 339/850 (39.88%), Postives = 494/850 (58.12%), Query Frame = 1

Query: 22  IKKLSSHALECLGMVCGLNDDLNKLRSNVSSIQSVLRDAEQRQIKGNDHSLTDWLEKLGD 81
           I KL S  ++C  M     DDL+KL  N+S I++V+ DAE++Q   N+H +  WLE L D
Sbjct: 14  IGKLGSVVVQCWNM----RDDLDKLVENMSEIKAVVLDAEEQQ-GTNNHQVQLWLENLKD 73

Query: 82  VFYDVEDVLDEISTEALRREVMTRGKNAKQVRIFFSNSNQLAFNYRMACQVKKINERLDV 141
            F D +D LD  +TE LRR+VMT  K AK+VRIFFS+SNQL F+Y+M  ++K++++R++ 
Sbjct: 74  AFDDADDFLDYFNTEELRRQVMTNHKKAKKVRIFFSSSNQLLFSYKMVQKIKELSKRIEA 133

Query: 142 ISQEKDKFQLNGIAYLGIQNVLSYPIGMERDTHSSLSGDQKIIGRDDEMNNLKKNLLAED 201
           ++ +K  F     A    Q VL      ER+THS +S +  +IGRD+E   L + L    
Sbjct: 134 LNVDKRVFNFTNRA--PEQRVL-----RERETHSFISAED-VIGRDEEKKELIELLFNTS 193

Query: 202 DKVKANVSFIAIVGMGGIGKTTLAKSLYNDKQVSDGFSSRIWIWVSNQFDTKTILKKIIE 261
           + VK NVS I+I+G+GG+GKT LA+ +YNDK+V + F  + W+ VS+ FD K I  KII+
Sbjct: 194 NNVKENVSVISIIGIGGLGKTALAQFVYNDKKVQEHFEFKKWVCVSDDFDVKGIAAKIIK 253

Query: 262 SATEKKPKVEEMEPLKTKLEEVIGGKKYLLVMDDVWNENENEWENLKNLLMLGARGSKVL 321
           S T       EME ++ +L   + GK+YLLV+DD WNEN N W  L  LL  GA GSK++
Sbjct: 254 SNT-----TAEMEEVQLELRNKVKGKRYLLVLDDNWNENRNLWLELMILLKDGAEGSKII 313

Query: 322 ITKRD---SKAVPGVETIPLKDLTEDFSWLLFKEVAFKESDLESINQNLIKMGKEISKRC 381
           IT R    +KA      + LK L+E  SW LF ++AF E+D E  N+ L+ +GKEI K+C
Sbjct: 314 ITARSEMVAKASGSSSILFLKGLSEKQSWTLFSQLAF-ENDRELENEELVSIGKEIVKKC 373

Query: 382 GGIPLVIRHIGRLLYGKTSAEDWEFIKENELLNVTREKNNNDGHVISTLKLSYNHLSPNL 441
            G+PL IR IG L+Y K   EDW   K  +L+ +  + +     ++  +KLSY+HL  +L
Sbjct: 374 AGVPLAIRSIGSLMYFK-EKEDWSTFKNKDLMQIDEQGDK----ILQLIKLSYDHLPFHL 433

Query: 442 KQCFSYSSLFPKGYKIRMNELIRQWIAQGFIESSNG-GKSVENIGKEYLDELCWRFFYEI 501
           K+CF++ SLFPK Y I    LIR WIAQGF++SS+    S+E+IG  Y  +L ++ F++ 
Sbjct: 434 KKCFAFCSLFPKDYFIPKTTLIRLWIAQGFVQSSDDESTSLEDIGHMYFMDLVYKSFFQN 493

Query: 502 SIEDVPFEEVG--MHDLMCDLAREVAGQKLYIRGYPESGYVVSEQTRHISFEYE-PRSWI 561
             ED  +  V   MHD+M DLA  ++     +    + G  + +Q RH+SF ++   SW 
Sbjct: 494 ITEDNFYGSVSCQMHDIMHDLASVISRNDCLL--VNKKGQHIDKQPRHVSFGFQLNHSWQ 553

Query: 562 DDVSKLQQAKGLRTFLLFTK--NPF--FTRNPIEKVLLDRLFSHFPRLRV-----LQIPN 621
              S L   K LRTFLL  K  N      R  IE    + + +   R RV     L + N
Sbjct: 554 VPTSLLNAYK-LRTFLLPLKWVNSMNGCDRCSIELCACNSILASSRRFRVLNLSFLNLTN 613

Query: 622 VSKSIKKLRHLRYLELG--EDAKSVPNSITKLQNLQTLDLTKCYDLKELPRDINNFVNLR 681
           +   I +++ LRYL+L      + +P SIT+L NL+TL L +C  L+ELP+D+   V+LR
Sbjct: 614 IPSCIGRMKQLRYLDLSCCFMVEELPRSITELVNLETLLLNRCSKLRELPKDLWKLVSLR 673

Query: 682 HLLCD--SRLMNMLQGTMEKLTSLQTLSSFLFDCKRFDKVKEFSERS--YFIEFDLKIKG 741
           HL  D    L +M +G + K+T+LQTL+ F+ D    D  K  SE    + +   L+I G
Sbjct: 674 HLELDYCHNLTSMPRG-IGKMTNLQTLTQFVLDTTSKDSAKT-SELGGLHNLRGLLEITG 733

Query: 742 LEQLRFSPSDVKSVNLKNKK-VPLLRLKWKFEN-GNEYEGDADDIVLEGLEPHPYVNLLQ 801
           LE LR  P++ K +NL+ K  +  L L WK +N G+  E + D+I+L+ +  H  +  L 
Sbjct: 734 LEHLRHCPTEAKPMNLRGKSHLDWLALNWKEDNVGDANELEKDEIILQDILLHSNIKTLI 793

Query: 802 IEGYCGVGLPNWVSTSILLRGIRIGNCDRLHLNQLSHLHA--LEILNLEGLKSVMSISEW 846
           I G+ GV L N V+    L  + + NC RL   QL+ LH   L + NL  L+ +++ S  
Sbjct: 794 ISGFGGVKLSNSVNLLTNLVDLNLYNCTRLQYIQLAPLHVKDLYMRNLPCLEYIVNDSNS 834

BLAST of Cucsa.128130 vs. TAIR10
Match: AT3G14470.1 (AT3G14470.1 NB-ARC domain-containing disease resistance protein)

HSP 1 Score: 345.1 bits (884), Expect = 1.3e-94
Identity = 241/823 (29.28%), Postives = 433/823 (52.61%), Query Frame = 1

Query: 39  LNDDL-NKLRSNVSSIQSVLRDAEQRQIKGNDHSLTDWLEKLGDVFYDVEDVLDEISTEA 98
           LN++L  +L + + +I +VL DAE++QI   +  +  W+ +L DV Y  ED LD+I+TEA
Sbjct: 34  LNENLLERLSTALLTITAVLIDAEEKQI--TNPVVEKWVNELRDVVYHAEDALDDIATEA 93

Query: 99  LRREVMTRGKNAKQVRIFFSNSNQLAF----NYRMACQVKKINERLDVISQEKDKFQLNG 158
           LR  +     ++ ++R      +   F    +  +  +++K+  RL+ ++ +++      
Sbjct: 94  LRLNIGAESSSSNRLRQLRGRMSLGDFLDGNSEHLETRLEKVTIRLERLASQRN------ 153

Query: 159 IAYLGIQNVLSYPIGMERDTHSSLSGDQKIIGRDDEMNNLKKNLLAEDDKVKANVSFIAI 218
              LG++  L+  I  +R   +SL  + ++ GRDD+ + + + L+ E+ K    ++ +AI
Sbjct: 154 --ILGLKE-LTAMIPKQRLPTTSLVDESEVFGRDDDKDEIMRFLIPENGKDNG-ITVVAI 213

Query: 219 VGMGGIGKTTLAKSLYNDKQVSDGFSSRIWIWVSNQFDTKTILKKIIESATEKKPKVEEM 278
           VG+GG+GKTTL++ LYND+ V   F +++W  VS +FD   I KK+ ES T +  +  ++
Sbjct: 214 VGIGGVGKTTLSQLLYNDQHVRSYFGTKVWAHVSEEFDVFKITKKVYESVTSRPCEFTDL 273

Query: 279 EPLKTKLEEVIGGK--KYLLVMDDVWNENENEWENLKNLLMLGARGSKVLITKRDSKAVP 338
           + L+ KL+E + G    +LLV+DD+WNEN  +W+ L+   +  A+GS++L+T R  +   
Sbjct: 274 DVLQVKLKERLTGTGLPFLLVLDDLWNENFADWDLLRQPFIHAAQGSQILVTTRSQRVAS 333

Query: 339 ---GVETIPLKDLTEDFSWLLFKEVAFKESDLESINQNLIKMGKEISKRCGGIPLVIRHI 398
               V    L+ L++   W LF +  F   +   +N+ +  + + I  +C G+PL ++ +
Sbjct: 334 IMCAVHVHNLQPLSDGDCWSLFMKTVFGNQE-PCLNREIGDLAERIVHKCRGLPLAVKTL 393

Query: 399 GRLLYGKTSAEDWEFIKENELLNVTREKNNNDGHVISTLKLSYNHLSPNLKQCFSYSSLF 458
           G +L  +    +WE +  + + ++  +K+N    ++  L++SY +L  +LK+CF+Y S+F
Sbjct: 394 GGVLRFEGKVIEWERVLSSRIWDLPADKSN----LLPVLRVSYYYLPAHLKRCFAYCSIF 453

Query: 459 PKGYKIRMNELIRQWIAQGFIESSNGGKSVENIGKEYLDELCWRFFYEISIEDVPFEEVG 518
           PKG+    ++++  W+A+GF++ +   K++E +G EY  EL  R     S+         
Sbjct: 454 PKGHAFEKDKVVLLWMAEGFLQQTRSSKNLEELGNEYFSELESR-----SLLQKTKTRYI 513

Query: 519 MHDLMCDLAREVAGQKLYIRGYPESG--YVVSEQTRHISFEYEPRSWIDDVSKLQQAKGL 578
           MHD + +LA+  +G+        E G    VSE+TR++S+  +  +   +   L++ K L
Sbjct: 514 MHDFINELAQFASGE---FSSKFEDGCKLQVSERTRYLSYLRDNYAEPMEFEALREVKFL 573

Query: 579 RTFLLFTKNPFFTRNPIEKVLLDRLFSHFPRLRVLQIPNVSKS------IKKLRHLRYLE 638
           RTFL  +         +++++ ++L     RLRVL + +   +       K + H R+L+
Sbjct: 574 RTFLPLSLTNSSRSCCLDQMVSEKLLPTLTRLRVLSLSHYKIARLPPDFFKNISHARFLD 633

Query: 639 LGE-DAKSVPNSITKLQNLQTLDLTKCYDLKELPRDINNFVNLRHLLCDSRLMNMLQGTM 698
           L   + + +P S+  + NLQTL L+ C  LKELP DI+N +NLR+L      +  +    
Sbjct: 634 LSRTELEKLPKSLCYMYNLQTLLLSYCSSLKELPTDISNLINLRYLDLIGTKLRQMPRRF 693

Query: 699 EKLTSLQTLSSFLFDCKRFDKVKEFSERSYFIEFDLKIKGLEQLRFSPSDVKSVNLKNKK 758
            +L SLQTL++F        ++ E     + +   LKI  L+++    +D    NL +KK
Sbjct: 694 GRLKSLQTLTTFFVSASDGSRISELG-GLHDLHGKLKIVELQRV-VDVADAAEANLNSKK 753

Query: 759 -VPLLRLKWKF------ENGNEYEGDADDIVLEGLEPHPYVNLLQIEGYCGVGLPNWVST 818
            +  +   W+        N N +    +  V E L PH ++  L IE Y G   P+W+S 
Sbjct: 754 HLREIDFVWRTGSSSSENNTNPHRTQNEAEVFEKLRPHRHIEKLAIERYKGRRFPDWLSD 813

Query: 819 SILLR--GIRIGNCDRL----HLNQLSHLHALEILNLEGLKSV 830
               R   IR+  C        L QL  L  L I  + GL+S+
Sbjct: 814 PSFSRIVCIRLRECQYCTSLPSLGQLPCLKELHISGMVGLQSI 829

BLAST of Cucsa.128130 vs. TAIR10
Match: AT3G14460.1 (AT3G14460.1 LRR and NB-ARC domains-containing disease resistance protein)

HSP 1 Score: 331.6 bits (849), Expect = 1.5e-90
Identity = 261/880 (29.66%), Postives = 430/880 (48.86%), Query Frame = 1

Query: 43  LNKLRSNVSSIQSVLRDAEQRQIKGNDHSLTDWLEKLGDVFYDVEDVLDEISTEALRREV 102
           L +L+  + +   VL DA+QR    +   +  WL  + D F+  ED+LDE+ TEALRR V
Sbjct: 36  LKRLKVALVTANPVLADADQRA--EHVREVKHWLTGIKDAFFQAEDILDELQTEALRRRV 95

Query: 103 MTRGKNAKQVRIFFSNSNQLAFNYRMACQVKKINERLDVISQEKDKFQLNGIAYLGIQNV 162
           +     A  +   F N        R A Q KKI  +++ + +  +   +  I  +G++  
Sbjct: 96  VAE---AGGLGGLFQN----LMAGREAIQ-KKIEPKMEKVVRLLEH-HVKHIEVIGLKEY 155

Query: 163 LSYPIGMERDTHSSLSGDQ---KIIGRDDEMNNLKKNLLAEDDKVKANVSFIAIVGMGGI 222
                   R    S   D    +++GR ++   L   LL++D+      + I++VGM G+
Sbjct: 156 SETREPQWRQASRSRPDDLPQGRLVGRVEDKLALVNLLLSDDEISIGKPAVISVVGMPGV 215

Query: 223 GKTTLAKSLYNDKQVSDGFSSRIWIWVSNQFDTKTILKKIIESATEKKPKVEEMEPLKTK 282
           GKTTL + ++ND +V++ F  ++WI     F+  T+ K +++  T      E++  L+ +
Sbjct: 216 GKTTLTEIVFNDYRVTEHFEVKMWISAGINFNVFTVTKAVLQDITSSAVNTEDLPSLQIQ 275

Query: 283 LEEVIGGKKYLLVMDDVWNENENEWENLKNLLMLGARGSKVLITKRD---SKAVPGVETI 342
           L++ + GK++LLV+DD W+E+++EWE+ +        GSK+++T R    S      +  
Sbjct: 276 LKKTLSGKRFLLVLDDFWSESDSEWESFQVAFTDAEEGSKIVLTTRSEIVSTVAKAEKIY 335

Query: 343 PLKDLTEDFSWLLFKEVAFKESDLESINQNLIKMGKEISKRCGGIPLVIRHIGRLLYGKT 402
            +K +T +  W L    AF    + SINQ L  +GK I+++C G+PL  R I   L  K 
Sbjct: 336 QMKLMTNEECWELISRFAFGNISVGSINQELEGIGKRIAEQCKGLPLAARAIASHLRSKP 395

Query: 403 SAEDWEFIKENELLNVTREKNNNDGHVISTLKLSYNHLSPNLKQCFSYSSLFPKGYKIRM 462
           + +DW  + +N         ++    ++  LKLSY+ L P LK+CF+  S+FPKG+    
Sbjct: 396 NPDDWYAVSKN--------FSSYTNSILPVLKLSYDSLPPQLKRCFALCSIFPKGHVFDR 455

Query: 463 NELIRQWIAQGFIESSNGGKSVENIGKEYLDELCWRFFYEISIEDVPFEEVGMHDLMCDL 522
            EL+  W+A   +      + +E+IG +YL +L  + F++    D+      MHDLM DL
Sbjct: 456 EELVLLWMAIDLLYQPRSSRRLEDIGNDYLGDLVAQSFFQRL--DITMTSFVMHDLMNDL 515

Query: 523 AREVAGQ---KLYIRGYPESGYVVSEQTRHISFEYEPRSWIDDVSKLQQAKGLRTFLLFT 582
           A+ V+G    +L     PE    +   TRH SF             +  A+ LRT L F 
Sbjct: 516 AKAVSGDFCFRLEDDNIPE----IPSTTRHFSFSRSQCDASVAFRSICGAEFLRTILPFN 575

Query: 583 KNPFFTRNPIEKVLLDRLFSHFPRLRVL-----QIPNVSKSIKKLRHLRYLELGE-DAKS 642
                    + + +L+ L +    LR+L     QI N+ KS+K L+ LRYL+L     K 
Sbjct: 576 SPTSLESLQLTEKVLNPLLNALSGLRILSLSHYQITNLPKSLKGLKLLRYLDLSSTKIKE 635

Query: 643 VPNSITKLQNLQTLDLTKCYDLKELPRDINNFVNLRHL-LCDSRLMNMLQGTMEKLTSLQ 702
           +P  +  L NLQTL L+ C DL  LP+ I   +NLR L L  + L+ M  G ++KL SLQ
Sbjct: 636 LPEFVCTLCNLQTLLLSNCRDLTSLPKSIAELINLRLLDLVGTPLVEMPPG-IKKLRSLQ 695

Query: 703 TLSSFLFDCKRFDKVKEFSERSYFIEFDLKIKGLEQLRFSPSDVKSVNLKNKK-VPLLRL 762
            LS+F+        + E  E S+ +   L+I  L+ + F+ S+ K   LK K  +  L L
Sbjct: 696 KLSNFVIGRLSGAGLHELKELSH-LRGTLRISELQNVAFA-SEAKDAGLKRKPFLDGLIL 755

Query: 763 KWKFENGNEYEGDADDI------VLEGLEPHPYVNLLQIEGYCGVGLPNWVSTSIL--LR 822
           KW  +      G  + +      VL  LEPHP++    IE Y G   P W+  S    + 
Sbjct: 756 KWTVKGSGFVPGSFNALACDQKEVLRMLEPHPHLKTFCIESYQGGAFPKWLGDSSFFGIT 815

Query: 823 GIRIGNCDR-LHLNQLSHLHALEILNLE--GLKSVMSISEWIGTLTSL-VSLEIEECPKL 882
            + + +C+  + L  +  L +L+ L++E   +   + +  + G   S  V  +  +  K 
Sbjct: 816 SVTLSSCNLCISLPPVGQLPSLKYLSIEKFNILQKVGLDFFFGENNSRGVPFQSLQILKF 875

Query: 883 KSLPK-------EMQQ--LKSLVQLNIIKCPQLGERCKEG 885
             +P+       E++      L +L I +CP L ++  EG
Sbjct: 876 YGMPRWDEWICPELEDGIFPCLQKLIIQRCPSLRKKFPEG 887

HSP 2 Score: 47.8 bits (112), Expect = 4.2e-05
Identity = 76/270 (28.15%), Postives = 114/270 (42.22%), Query Frame = 1

Query: 636  QNLQTLDLTKCYDLKELPRDIN-NFVNLRHLL---CDSRLMNMLQGTMEKLTSLQTLSSF 695
            QNLQ+L +  C  L  LP ++  ++ NL  LL   C S  +    G+    T+L+TL  +
Sbjct: 1091 QNLQSLHIDSCDGLTSLPENLTESYPNLHELLIIACHS--LESFPGSHPP-TTLKTL--Y 1150

Query: 696  LFDCKRFDKVKEFSE-RSYFIEFDLKIKGLEQLRFSPSDVKSVNLKNKKVPLLRLKW--- 755
            + DCK+ +  +     RSY          LE L    S    VN      P LR      
Sbjct: 1151 IRDCKKLNFTESLQPTRSY--------SQLEYLFIGSSCSNLVNFPLSLFPKLRSLSIRD 1210

Query: 756  --KFENGNEYEGDADD-IVLEGLEPHPYVNLLQIEGYCGVGLPNWVSTSILLRGIRIGNC 815
               F+  + + G  DD I LE LE     NL   E +   GLP    +S+LL      NC
Sbjct: 1211 CESFKTFSIHAGLGDDRIALESLEIRDCPNL---ETFPQGGLPTPKLSSMLL-----SNC 1270

Query: 816  DRLHLNQLSHLHALEILNLEGLKSVMSISEWIGTLTSLVSLEIEECPKLKSLPKEMQQLK 875
                                  K + ++ E +  LTSL+SL I +CP+++++P       
Sbjct: 1271 ----------------------KKLQALPEKLFGLTSLLSLFIIKCPEIETIPGGGFP-S 1316

Query: 876  SLVQLNIIKCPQLGERCKEGGEDWPNISHI 895
            +L  L I  C +L  R + G  D  N+ ++
Sbjct: 1331 NLRTLCISLCDKLTPRIEWGLRDLENLRNL 1316

BLAST of Cucsa.128130 vs. TAIR10
Match: AT3G46730.1 (AT3G46730.1 NB-ARC domain-containing disease resistance protein)

HSP 1 Score: 250.0 bits (637), Expect = 5.6e-66
Identity = 213/752 (28.32%), Postives = 374/752 (49.73%), Query Frame = 1

Query: 22  IKKLSSHALECLGMVCGLNDDLNKLRSNVSSIQSVLRDAEQRQIKGNDHSLTDWLEKLGD 81
           + K+  + +  +  + G+ DDL +L++ ++ I   L+D E R+ +  D    +W + + D
Sbjct: 10  LNKIGGYLINEVLALMGVKDDLEELKTELTCIHGYLKDVEARERE--DEVSKEWTKLVLD 69

Query: 82  VFYDVEDVLD----EISTEALRREVMTRGKNAKQVRIFFSNSNQLAFNYRMACQVKKINE 141
           + YD+EDVLD    ++   +LRR ++       + R            Y +   ++ +  
Sbjct: 70  IAYDIEDVLDTYFLKLEERSLRRGLLRLTNKIGKKRDA----------YNIVEDIRTLKR 129

Query: 142 RLDVISQEKDKFQLNGIAYLGIQNVLSYPIGMERDTHSSLSGDQKIIGRDDEMNNLKKNL 201
           R+  I+++++ F +        +N+ +  +   R     +  ++ ++G +D++  L   L
Sbjct: 130 RILDITRKRETFGIGSFNEPRGENITNVRVRQLRRA-PPVDQEELVVGLEDDVKILLVKL 189

Query: 202 LAEDDKVKANVSFIAIVGMGGIGKTTLAKSLYNDKQVSDGFSSRIWIWVSNQFDTKTILK 261
           L++++K K+ +  I+I GMGG+GKT LA+ LYN   V   F  R W +VS ++ T+ IL 
Sbjct: 190 LSDNEKDKSYI--ISIFGMGGLGKTALARKLYNSGDVKRRFDCRAWTYVSQEYKTRDILI 249

Query: 262 KIIES-------ATEKKPKVEEMEPLKTKLEEVIGGKKYLLVMDDVWNENENEWENLKNL 321
           +II S         EK    EE E L+  L  ++ GK Y++V+DDVW  + + WE+LK  
Sbjct: 250 RIIRSLGIVSAEEMEKIKMFEEDEELEVYLYGLLEGKNYMVVVDDVW--DPDAWESLKRA 309

Query: 322 LMLGARGSKVLITKRDSKAVPGVE----TIPLKDLTEDFSWLLFKEVAFKESDLESINQN 381
           L    RGSKV+IT R      GVE       L+ LT + SW LF+  AF  S++E ++++
Sbjct: 310 LPCDHRGSKVIITTRIRAIAEGVEGTVYAHKLRFLTFEESWTLFERKAF--SNIEKVDED 369

Query: 382 LIKMGKEISKRCGGIPLVIRHIGRLLYGKTSAEDWEFIKENELLNVTREKNNNDGHVIST 441
           L + GKE+ K+CGG+PL I  +  LL  K + E W  +      ++ R   +N  H+ + 
Sbjct: 370 LQRTGKEMVKKCGGLPLAIVVLSGLLSRKRTNE-WHEV----CASLWRRLKDNSIHISTV 429

Query: 442 LKLSYNHLSPNLKQCFSYSSLFPKGYKIRMNELIRQWIAQGFIESSNGGKSVENIGKEYL 501
             LS+  +   LK CF Y S+FP+ Y+I++ +LI   +A+GFI+       +E++ + Y+
Sbjct: 430 FDLSFKEMRHELKLCFLYFSVFPEDYEIKVEKLIHLLVAEGFIQEDE-EMMMEDVARCYI 489

Query: 502 DELCWRFFYEIS-IEDVPFEEVGMHDLMCDLAREVAGQKLYIRGYPE----SGYVVSEQT 561
           DEL  R   +   IE        +HDL+ DLA + A +  ++  Y E    S     E  
Sbjct: 490 DELVDRSLVKAERIERGKVMSCRIHDLLRDLAIKKAKELNFVNVYNEKQHSSDICRREVV 549

Query: 562 RHISFEYEPRSWIDDVSKLQQAKGLRTFLLFTKNPFF--TRNPIEKVLLDRLFSHFPRLR 621
            H+  +Y        +   +  K +R+FL   +   F        K+ L R+ +    L 
Sbjct: 550 HHLMNDYY-------LCDRRVNKRMRSFLFIGERRGFGYVNTTNLKLKLLRVLNMEGLLF 609

Query: 622 VLQIPNVSKS----IKKLRHLRYLELGEDAKSV-PNSITKLQNLQTLDLTKCYDLKELPR 681
           V +  N+S +    I +L HLRYL + +   S+ P SI+ L+ LQTLD +   D  +   
Sbjct: 610 VSK--NISNTLPDVIGELIHLRYLGIADTYVSILPASISNLRFLQTLDASG-NDPFQYTT 669

Query: 682 DINNFVNLRHLLCDSRLMNMLQGTMEKLTSLQTLSSFLFDCKRFDKVKEFSERSYFIEFD 741
           D++   +LRH++    +   L G    L +L+++SS+ +     + ++   +    +E  
Sbjct: 670 DLSKLTSLRHVI-GKFVGECLIGEGVNLQTLRSISSYSWSKLNHELLRNLQD----LEIY 721

Query: 742 LKIKGLEQLRFSPSDVKSVNLKNKKVPLLRLK 747
              K ++Q R   + V     KN +V  L ++
Sbjct: 730 DHSKWVDQRRVPLNFVSFSKPKNLRVLKLEMR 721

BLAST of Cucsa.128130 vs. TAIR10
Match: AT3G46530.1 (AT3G46530.1 NB-ARC domain-containing disease resistance protein)

HSP 1 Score: 229.2 bits (583), Expect = 1.0e-59
Identity = 200/696 (28.74%), Postives = 351/696 (50.43%), Query Frame = 1

Query: 22  IKKLSSHALECLGMVCGLNDDLNKLRSNVSSIQSVLRDAEQRQIKGNDHSLTDWLEKLGD 81
           + K+ ++ +E   M   + +DL +L++ ++ I   L+D E R+ +  D    +W + + D
Sbjct: 10  VGKIGNYLIEEASMFMAVKEDLEELKTELTCIHGYLKDVEARERE--DEVSKEWSKLVLD 69

Query: 82  VFYDVEDVLDEISTEALRREVMTRGKNAKQVRIFFSNSNQLAFNYRMACQVKKINERLDV 141
             YDVEDVLD   T  L+ E  ++ +  +++    +   +    Y +   ++ +  R+  
Sbjct: 70  FAYDVEDVLD---TYHLKLEERSQRRGLRRLT---NKIGRKMDAYSIVDDIRILKRRILD 129

Query: 142 ISQEKDKFQLNGIAY-LGIQNVLSYPIGMERDTHSSLSGDQKIIGRDDEMNNLKKNLLAE 201
           I+++++ + + G+    G  N  S  +   R   S +  ++ ++G +D+   L + LL  
Sbjct: 130 ITRKRETYGIGGLKEPQGGGNTSSLRVRQLRRARS-VDQEEVVVGLEDDAKILLEKLLDY 189

Query: 202 DDKVKANVSFIAIVGMGGIGKTTLAKSLYNDKQVSDGFSSRIWIWVSNQFDTKTILKKII 261
           ++K   N   I+I GMGG+GKT LA+ LYN + V + F  R W +VS ++ T  IL +II
Sbjct: 190 EEK---NRFIISIFGMGGLGKTALARKLYNSRDVKERFEYRAWTYVSQEYKTGDILMRII 249

Query: 262 ES----ATEKKPKVEEM--EPLKTKLEEVIGGKKYLLVMDDVWNENENEWENLKNLLMLG 321
            S    + E+  K+ +   E L+  L  ++ GKKYL+V+DD+W      W++LK  L   
Sbjct: 250 RSLGMTSGEELEKIRKFAEEELEVYLYGLLEGKKYLVVVDDIW--EREAWDSLKRALPCN 309

Query: 322 ARGSKVLITKRDSKAVPGVE----TIPLKDLTEDFSWLLFKEVAFKESDLESINQNLIKM 381
             GS+V+IT R      GV+       L+ LT + SW LF++ AF+  +++  +++L+K 
Sbjct: 310 HEGSRVIITTRIKAVAEGVDGRFYAHKLRFLTFEESWELFEQRAFR--NIQRKDEDLLKT 369

Query: 382 GKEISKRCGGIPLVIRHIGRLLYGKTSAEDWEFIKENELLN-VTREKNNNDGHVIS-TLK 441
           GKE+ ++C G+PL I  +  LL  KT +E W     N++ N + R   ++  HV      
Sbjct: 370 GKEMVQKCRGLPLCIVVLAGLLSRKTPSE-W-----NDVCNSLWRRLKDDSIHVAPIVFD 429

Query: 442 LSYNHLSPNLKQCFSYSSLFPKGYKIRMNELIRQWIAQGFIESSNGGKSVENIGKEYLDE 501
           LS+  L    K CF Y S+FP+ Y+I + +LI   +A+GFI+       +E++ + Y++E
Sbjct: 430 LSFKELRHESKLCFLYLSIFPEDYEIDLEKLIHLLVAEGFIQGDE-EMMMEDVARYYIEE 489

Query: 502 LCWRFFYE-ISIEDVPFEEVGMHDLMCDLAREVAGQKLYIRGYPESGYVVSEQTRHISFE 561
           L  R   E +  E        +HDL+ D+A + + +  ++  Y  + +V    +     E
Sbjct: 490 LIDRSLLEAVRRERGKVMSCRIHDLLRDVAIKKSKELNFVNVY--NDHVAQHSSTTCRRE 549

Query: 562 YEPRSWIDDVSKLQQAKGLRTFLLFTKNPFFTRNPIEKVLLDRLFSHFPRLRVLQIPNVS 621
                +    S+ ++ K +R+FL F +         E + L         LRVL   ++ 
Sbjct: 550 VVHHQFKRYSSEKRKNKRMRSFLYFGEFDHLVGLDFETLKL---------LRVLDFGSLW 609

Query: 622 KSIK---KLRHLRYLELGEDAKSVPN-----SITKLQNLQTLDLTKCYDLKELPRDINNF 681
              K    L HLRY  LG D  S+ +      I+KL+ LQTL ++  Y ++E   D+   
Sbjct: 610 LPFKINGDLIHLRY--LGIDGNSINDFDIAAIISKLRFLQTLFVSDNYFIEE-TIDLRKL 663

Query: 682 VNLRHLLCDSRLMNMLQGTM-EKLTSLQTLSSFLFD 695
            +LRH+     + N   G +   + +LQTL+S  FD
Sbjct: 670 TSLRHV-----IGNFFGGLLIGDVANLQTLTSISFD 663

BLAST of Cucsa.128130 vs. TAIR10
Match: AT1G58400.1 (AT1G58400.1 Disease resistance protein (CC-NBS-LRR class) family)

HSP 1 Score: 223.0 bits (567), Expect = 7.3e-58
Identity = 234/937 (24.97%), Postives = 420/937 (44.82%), Query Frame = 1

Query: 19  SHGIKKLSSHALECLGMVCGLNDDLNKLRSNVSSIQSVLRDAEQRQIKGNDHSLTDWLEK 78
           S G++KL     +      G+ D + +L+SN++ ++S L+DAE +  K     +   +E+
Sbjct: 7   SFGVEKLWDRLTQEYEQFQGVEDRIAELKSNLNLLKSFLKDAEAK--KNTSQMVRHCVEE 66

Query: 79  LGDVFYDVEDVLDE-ISTEALRREVMTRGKNAKQVRIFFSNSNQLAFNYRMACQVKKINE 138
           + ++ YD E++++  I  EA R       K +  +R     +      +  A  +  I++
Sbjct: 67  IKEIVYDTENMIETFILKEAAR-------KRSGIIRRITKLTCIKVHRWEFASDIGGISK 126

Query: 139 RLDVISQEKDKFQLNGIAYLGIQNVLSYPIG-MERDTHSSLSG--DQKIIGRDDEMNNLK 198
           R+  + Q+   F +  +   G Q+  S+ +   ER+   + S   +   +G +  +  L 
Sbjct: 127 RISKVIQDMHSFGVQQMISDGSQS--SHLLQEREREMRQTFSRGYESDFVGLEVNVKKLV 186

Query: 199 KNLLAEDDKVKANVSFIAIVGMGGIGKTTLAKSLYNDKQVSDGFSSRIWIWVSNQFDTKT 258
             L+ EDD     +  +++ GMGG+GKTTLA+ ++N + V   F    W+ VS +F  K 
Sbjct: 187 GYLVEEDD-----IQIVSVTGMGGLGKTTLARQVFNHEDVKHQFDRLAWVCVSQEFTRKN 246

Query: 259 ILKKIIESATEKKPKVE----EMEPLKTKLEEVIGGKKYLLVMDDVWNENENEWENLKNL 318
           + + I+++ T ++ K E    E   L  +L +++   K L+V DD+W   E +W  +   
Sbjct: 247 VWQMILQNLTSRETKDEILQMEEAELHDELFQLLETSKSLIVFDDIW--KEEDWGLI--- 306

Query: 319 LMLGARGSKVLITKRDSKAVPG---VETIPLKDLTEDFSWLLFKEVAFKESDLE--SINQ 378
                  + +   K+++ A+ G         + LT   SW+LF+ +A    D     +++
Sbjct: 307 -------NPIFPPKKETIAMHGNRRYVNFKPECLTILESWILFQRIAMPRVDESEFKVDK 366

Query: 379 NLIKMGKEISKRCGGIPLVIRHIGRLLYGKTSAEDWEFIKEN---ELLNVTREKNNNDGH 438
            +  MGK++ K CGG+PL ++ +G LL  K +  DW+ + EN    ++  T   + N+  
Sbjct: 367 EMEMMGKQMIKYCGGLPLAVKVLGGLLAAKYTFHDWKRLSENIGCHIVGRTDFSDGNNSS 426

Query: 439 VISTLKLSYNHLSPNLKQCFSYSSLFPKGYKIRMNELIRQWIAQGFIESSN-GGKSVENI 498
           V   L LS+  L   LK CF Y + FP+ + I++ +L   W A+G +E  +  G+++ ++
Sbjct: 427 VYHVLSLSFEELPSYLKHCFLYLAHFPEDHNIKVEKLSYCWAAEGILEPRHYHGQTIRDV 486

Query: 499 GKEYLDELCWR--FFYEISIEDVPFEEVGMHDLMCDLAREVAGQKLYIRGYPESGYVVSE 558
           G+ Y++EL  R     E  +  + FE   +HD+M    REV   K     + +   ++  
Sbjct: 487 GESYIEELVRRNMVIAERDVTTLRFEACHLHDMM----REVCLLKAKEENFVQIASIL-P 546

Query: 559 QTRHISFEYEPRSWIDD------VSKLQQAKGLRTFLLFTKNPFFTRNPIEKVLLDRLFS 618
            T +  +    R ++        VS+      L++ L+  +N    R    K LL   F 
Sbjct: 547 PTANSQYPGTSRRFVSQNPTTLHVSRDINNPKLQSLLIVWEN----RRKSWK-LLGSSFI 606

Query: 619 HFPRLRVLQI-------PNVSKSIKKLRHLRYLELG-EDAKSVPNSITKLQNLQTLDLTK 678
               LRVL +        N+   I KL HLRYL L       +P+S+  L+ L  LD+  
Sbjct: 607 RLELLRVLDLYKAKFEGRNLPSGIGKLIHLRYLNLDLARVSRLPSSLGNLRLLIYLDINV 666

Query: 679 CYDLKELPRDINNFVNLRHLLCDSRLMNMLQGTMEKLTSLQTLSSFLFDCKRFDKV---- 738
           C     +P  +     LR+L         ++  +  L +L+TL +F  +    + +    
Sbjct: 667 CTKSLFVPNCLMGMHELRYLRLPFNTSKEIKLGLCNLVNLETLENFSTENSSLEDLRGMV 726

Query: 739 ----------KEFSERSYFIEFDLKIKGLEQLRFSPSD--VKSVNLKNKKVPLLRLKWKF 798
                     K  S+ + F    L ++ LE L     D   K   +    + L  +  K 
Sbjct: 727 SLRTLTIGLFKHISKETLFASI-LGMRHLENLSIRTPDGSSKFKRIMEDGIVLDAIHLKQ 786

Query: 799 ENGNEYEGDADDIVLEGLEPH--PYVNLLQIEGYCGVGLPNWVSTSIL-LRGIRI---GN 858
            N   Y     D      E H   ++  + ++G C V  P  +   +L L+ +R+     
Sbjct: 787 LNLRLYMPKLPD------EQHFPSHLTSISLDGCCLVEDPLPILEKLLELKEVRLDFRAF 846

Query: 859 CDRLHLNQLSHLHALEILNLEGLKSVMSISEWI---GTLTSLVSLEIEECPKLKSLPKEM 898
           C +  ++       L  L + GL       EWI   G++  L +L I  C KLK LP  +
Sbjct: 847 CGKRMVSSDGGFPQLHRLYIWGL---AEWEEWIVEEGSMPRLHTLTIWNCQKLKQLPDGL 893

BLAST of Cucsa.128130 vs. NCBI nr
Match: gi|778728511|ref|XP_011659431.1| (PREDICTED: disease resistance protein RGA2-like isoform X1 [Cucumis sativus])

HSP 1 Score: 1758.8 bits (4554), Expect = 0.0e+00
Identity = 878/879 (99.89%), Postives = 879/879 (100.00%), Query Frame = 1

Query: 22  IKKLSSHALECLGMVCGLNDDLNKLRSNVSSIQSVLRDAEQRQIKGNDHSLTDWLEKLGD 81
           +KKLSSHALECLGMVCGLNDDLNKLRSNVSSIQSVLRDAEQRQIKGNDHSLTDWLEKLGD
Sbjct: 15  LKKLSSHALECLGMVCGLNDDLNKLRSNVSSIQSVLRDAEQRQIKGNDHSLTDWLEKLGD 74

Query: 82  VFYDVEDVLDEISTEALRREVMTRGKNAKQVRIFFSNSNQLAFNYRMACQVKKINERLDV 141
           VFYDVEDVLDEISTEALRREVMTRGKNAKQVRIFFSNSNQLAFNYRMACQVKKINERLDV
Sbjct: 75  VFYDVEDVLDEISTEALRREVMTRGKNAKQVRIFFSNSNQLAFNYRMACQVKKINERLDV 134

Query: 142 ISQEKDKFQLNGIAYLGIQNVLSYPIGMERDTHSSLSGDQKIIGRDDEMNNLKKNLLAED 201
           ISQEKDKFQLNGIAYLGIQNVLSYPIGMERDTHSSLSGDQKIIGRDDEMNNLKKNLLAED
Sbjct: 135 ISQEKDKFQLNGIAYLGIQNVLSYPIGMERDTHSSLSGDQKIIGRDDEMNNLKKNLLAED 194

Query: 202 DKVKANVSFIAIVGMGGIGKTTLAKSLYNDKQVSDGFSSRIWIWVSNQFDTKTILKKIIE 261
           DKVKANVSFIAIVGMGGIGKTTLAKSLYNDKQVSDGFSSRIWIWVSNQFDTKTILKKIIE
Sbjct: 195 DKVKANVSFIAIVGMGGIGKTTLAKSLYNDKQVSDGFSSRIWIWVSNQFDTKTILKKIIE 254

Query: 262 SATEKKPKVEEMEPLKTKLEEVIGGKKYLLVMDDVWNENENEWENLKNLLMLGARGSKVL 321
           SATEKKPKVEEMEPLKTKLEEVIGGKKYLLVMDDVWNENENEWENLKNLLMLGARGSKVL
Sbjct: 255 SATEKKPKVEEMEPLKTKLEEVIGGKKYLLVMDDVWNENENEWENLKNLLMLGARGSKVL 314

Query: 322 ITKRDSKAVPGVETIPLKDLTEDFSWLLFKEVAFKESDLESINQNLIKMGKEISKRCGGI 381
           ITKRDSKAVPGVETIPLKDLTEDFSWLLFKEVAFKESDLESINQNLIKMGKEISKRCGGI
Sbjct: 315 ITKRDSKAVPGVETIPLKDLTEDFSWLLFKEVAFKESDLESINQNLIKMGKEISKRCGGI 374

Query: 382 PLVIRHIGRLLYGKTSAEDWEFIKENELLNVTREKNNNDGHVISTLKLSYNHLSPNLKQC 441
           PLVIRHIGRLLYGKTSAEDWEFIKENELLNVTREKNNNDGHVISTLKLSYNHLSPNLKQC
Sbjct: 375 PLVIRHIGRLLYGKTSAEDWEFIKENELLNVTREKNNNDGHVISTLKLSYNHLSPNLKQC 434

Query: 442 FSYSSLFPKGYKIRMNELIRQWIAQGFIESSNGGKSVENIGKEYLDELCWRFFYEISIED 501
           FSYSSLFPKGYKIRMNELIRQWIAQGFIESSNGGKSVENIGKEYLDELCWRFFYEISIED
Sbjct: 435 FSYSSLFPKGYKIRMNELIRQWIAQGFIESSNGGKSVENIGKEYLDELCWRFFYEISIED 494

Query: 502 VPFEEVGMHDLMCDLAREVAGQKLYIRGYPESGYVVSEQTRHISFEYEPRSWIDDVSKLQ 561
           VPFEEVGMHDLMCDLAREVAGQKLYIRGYPESGYVVSEQTRHISFEYEPRSWIDDVSKLQ
Sbjct: 495 VPFEEVGMHDLMCDLAREVAGQKLYIRGYPESGYVVSEQTRHISFEYEPRSWIDDVSKLQ 554

Query: 562 QAKGLRTFLLFTKNPFFTRNPIEKVLLDRLFSHFPRLRVLQIPNVSKSIKKLRHLRYLEL 621
           QAKGLRTFLLFTKNPFFTRNPIEKVLLDRLFSHFPRLRVLQIPNVSKSIKKLRHLRYLEL
Sbjct: 555 QAKGLRTFLLFTKNPFFTRNPIEKVLLDRLFSHFPRLRVLQIPNVSKSIKKLRHLRYLEL 614

Query: 622 GEDAKSVPNSITKLQNLQTLDLTKCYDLKELPRDINNFVNLRHLLCDSRLMNMLQGTMEK 681
           GEDAKSVPNSITKLQNLQTLDLTKCYDLKELPRDINNFVNLRHLLCDSRLMNMLQGTMEK
Sbjct: 615 GEDAKSVPNSITKLQNLQTLDLTKCYDLKELPRDINNFVNLRHLLCDSRLMNMLQGTMEK 674

Query: 682 LTSLQTLSSFLFDCKRFDKVKEFSERSYFIEFDLKIKGLEQLRFSPSDVKSVNLKNKKVP 741
           LTSLQTLSSFLFDCKRFDKVKEFSERSYFIEFDLKIKGLEQLRFSPSDVKSVNLKNKKVP
Sbjct: 675 LTSLQTLSSFLFDCKRFDKVKEFSERSYFIEFDLKIKGLEQLRFSPSDVKSVNLKNKKVP 734

Query: 742 LLRLKWKFENGNEYEGDADDIVLEGLEPHPYVNLLQIEGYCGVGLPNWVSTSILLRGIRI 801
           LLRLKWKFENGNEYEGDADDIVLEGLEPHPYVNLLQIEGYCGVGLPNWVSTSILLRGIRI
Sbjct: 735 LLRLKWKFENGNEYEGDADDIVLEGLEPHPYVNLLQIEGYCGVGLPNWVSTSILLRGIRI 794

Query: 802 GNCDRLHLNQLSHLHALEILNLEGLKSVMSISEWIGTLTSLVSLEIEECPKLKSLPKEMQ 861
           GNCDRLHLNQLSHLHALEILNLEGLKSVMSISEWIGTLTSLVSLEIEECPKLKSLPKEMQ
Sbjct: 795 GNCDRLHLNQLSHLHALEILNLEGLKSVMSISEWIGTLTSLVSLEIEECPKLKSLPKEMQ 854

Query: 862 QLKSLVQLNIIKCPQLGERCKEGGEDWPNISHIPDVLID 901
           QLKSLVQLNIIKCPQLGERCKEGGEDWPNISHIPDVLID
Sbjct: 855 QLKSLVQLNIIKCPQLGERCKEGGEDWPNISHIPDVLID 893

BLAST of Cucsa.128130 vs. NCBI nr
Match: gi|778728514|ref|XP_011659432.1| (PREDICTED: disease resistance protein RGA2-like isoform X2 [Cucumis sativus])

HSP 1 Score: 1684.8 bits (4362), Expect = 0.0e+00
Identity = 840/840 (100.00%), Postives = 840/840 (100.00%), Query Frame = 1

Query: 61  EQRQIKGNDHSLTDWLEKLGDVFYDVEDVLDEISTEALRREVMTRGKNAKQVRIFFSNSN 120
           EQRQIKGNDHSLTDWLEKLGDVFYDVEDVLDEISTEALRREVMTRGKNAKQVRIFFSNSN
Sbjct: 5   EQRQIKGNDHSLTDWLEKLGDVFYDVEDVLDEISTEALRREVMTRGKNAKQVRIFFSNSN 64

Query: 121 QLAFNYRMACQVKKINERLDVISQEKDKFQLNGIAYLGIQNVLSYPIGMERDTHSSLSGD 180
           QLAFNYRMACQVKKINERLDVISQEKDKFQLNGIAYLGIQNVLSYPIGMERDTHSSLSGD
Sbjct: 65  QLAFNYRMACQVKKINERLDVISQEKDKFQLNGIAYLGIQNVLSYPIGMERDTHSSLSGD 124

Query: 181 QKIIGRDDEMNNLKKNLLAEDDKVKANVSFIAIVGMGGIGKTTLAKSLYNDKQVSDGFSS 240
           QKIIGRDDEMNNLKKNLLAEDDKVKANVSFIAIVGMGGIGKTTLAKSLYNDKQVSDGFSS
Sbjct: 125 QKIIGRDDEMNNLKKNLLAEDDKVKANVSFIAIVGMGGIGKTTLAKSLYNDKQVSDGFSS 184

Query: 241 RIWIWVSNQFDTKTILKKIIESATEKKPKVEEMEPLKTKLEEVIGGKKYLLVMDDVWNEN 300
           RIWIWVSNQFDTKTILKKIIESATEKKPKVEEMEPLKTKLEEVIGGKKYLLVMDDVWNEN
Sbjct: 185 RIWIWVSNQFDTKTILKKIIESATEKKPKVEEMEPLKTKLEEVIGGKKYLLVMDDVWNEN 244

Query: 301 ENEWENLKNLLMLGARGSKVLITKRDSKAVPGVETIPLKDLTEDFSWLLFKEVAFKESDL 360
           ENEWENLKNLLMLGARGSKVLITKRDSKAVPGVETIPLKDLTEDFSWLLFKEVAFKESDL
Sbjct: 245 ENEWENLKNLLMLGARGSKVLITKRDSKAVPGVETIPLKDLTEDFSWLLFKEVAFKESDL 304

Query: 361 ESINQNLIKMGKEISKRCGGIPLVIRHIGRLLYGKTSAEDWEFIKENELLNVTREKNNND 420
           ESINQNLIKMGKEISKRCGGIPLVIRHIGRLLYGKTSAEDWEFIKENELLNVTREKNNND
Sbjct: 305 ESINQNLIKMGKEISKRCGGIPLVIRHIGRLLYGKTSAEDWEFIKENELLNVTREKNNND 364

Query: 421 GHVISTLKLSYNHLSPNLKQCFSYSSLFPKGYKIRMNELIRQWIAQGFIESSNGGKSVEN 480
           GHVISTLKLSYNHLSPNLKQCFSYSSLFPKGYKIRMNELIRQWIAQGFIESSNGGKSVEN
Sbjct: 365 GHVISTLKLSYNHLSPNLKQCFSYSSLFPKGYKIRMNELIRQWIAQGFIESSNGGKSVEN 424

Query: 481 IGKEYLDELCWRFFYEISIEDVPFEEVGMHDLMCDLAREVAGQKLYIRGYPESGYVVSEQ 540
           IGKEYLDELCWRFFYEISIEDVPFEEVGMHDLMCDLAREVAGQKLYIRGYPESGYVVSEQ
Sbjct: 425 IGKEYLDELCWRFFYEISIEDVPFEEVGMHDLMCDLAREVAGQKLYIRGYPESGYVVSEQ 484

Query: 541 TRHISFEYEPRSWIDDVSKLQQAKGLRTFLLFTKNPFFTRNPIEKVLLDRLFSHFPRLRV 600
           TRHISFEYEPRSWIDDVSKLQQAKGLRTFLLFTKNPFFTRNPIEKVLLDRLFSHFPRLRV
Sbjct: 485 TRHISFEYEPRSWIDDVSKLQQAKGLRTFLLFTKNPFFTRNPIEKVLLDRLFSHFPRLRV 544

Query: 601 LQIPNVSKSIKKLRHLRYLELGEDAKSVPNSITKLQNLQTLDLTKCYDLKELPRDINNFV 660
           LQIPNVSKSIKKLRHLRYLELGEDAKSVPNSITKLQNLQTLDLTKCYDLKELPRDINNFV
Sbjct: 545 LQIPNVSKSIKKLRHLRYLELGEDAKSVPNSITKLQNLQTLDLTKCYDLKELPRDINNFV 604

Query: 661 NLRHLLCDSRLMNMLQGTMEKLTSLQTLSSFLFDCKRFDKVKEFSERSYFIEFDLKIKGL 720
           NLRHLLCDSRLMNMLQGTMEKLTSLQTLSSFLFDCKRFDKVKEFSERSYFIEFDLKIKGL
Sbjct: 605 NLRHLLCDSRLMNMLQGTMEKLTSLQTLSSFLFDCKRFDKVKEFSERSYFIEFDLKIKGL 664

Query: 721 EQLRFSPSDVKSVNLKNKKVPLLRLKWKFENGNEYEGDADDIVLEGLEPHPYVNLLQIEG 780
           EQLRFSPSDVKSVNLKNKKVPLLRLKWKFENGNEYEGDADDIVLEGLEPHPYVNLLQIEG
Sbjct: 665 EQLRFSPSDVKSVNLKNKKVPLLRLKWKFENGNEYEGDADDIVLEGLEPHPYVNLLQIEG 724

Query: 781 YCGVGLPNWVSTSILLRGIRIGNCDRLHLNQLSHLHALEILNLEGLKSVMSISEWIGTLT 840
           YCGVGLPNWVSTSILLRGIRIGNCDRLHLNQLSHLHALEILNLEGLKSVMSISEWIGTLT
Sbjct: 725 YCGVGLPNWVSTSILLRGIRIGNCDRLHLNQLSHLHALEILNLEGLKSVMSISEWIGTLT 784

Query: 841 SLVSLEIEECPKLKSLPKEMQQLKSLVQLNIIKCPQLGERCKEGGEDWPNISHIPDVLID 900
           SLVSLEIEECPKLKSLPKEMQQLKSLVQLNIIKCPQLGERCKEGGEDWPNISHIPDVLID
Sbjct: 785 SLVSLEIEECPKLKSLPKEMQQLKSLVQLNIIKCPQLGERCKEGGEDWPNISHIPDVLID 844

BLAST of Cucsa.128130 vs. NCBI nr
Match: gi|700189864|gb|KGN45097.1| (hypothetical protein Csa_7G425890 [Cucumis sativus])

HSP 1 Score: 1077.8 bits (2786), Expect = 0.0e+00
Identity = 531/531 (100.00%), Postives = 531/531 (100.00%), Query Frame = 1

Query: 370 MGKEISKRCGGIPLVIRHIGRLLYGKTSAEDWEFIKENELLNVTREKNNNDGHVISTLKL 429
           MGKEISKRCGGIPLVIRHIGRLLYGKTSAEDWEFIKENELLNVTREKNNNDGHVISTLKL
Sbjct: 1   MGKEISKRCGGIPLVIRHIGRLLYGKTSAEDWEFIKENELLNVTREKNNNDGHVISTLKL 60

Query: 430 SYNHLSPNLKQCFSYSSLFPKGYKIRMNELIRQWIAQGFIESSNGGKSVENIGKEYLDEL 489
           SYNHLSPNLKQCFSYSSLFPKGYKIRMNELIRQWIAQGFIESSNGGKSVENIGKEYLDEL
Sbjct: 61  SYNHLSPNLKQCFSYSSLFPKGYKIRMNELIRQWIAQGFIESSNGGKSVENIGKEYLDEL 120

Query: 490 CWRFFYEISIEDVPFEEVGMHDLMCDLAREVAGQKLYIRGYPESGYVVSEQTRHISFEYE 549
           CWRFFYEISIEDVPFEEVGMHDLMCDLAREVAGQKLYIRGYPESGYVVSEQTRHISFEYE
Sbjct: 121 CWRFFYEISIEDVPFEEVGMHDLMCDLAREVAGQKLYIRGYPESGYVVSEQTRHISFEYE 180

Query: 550 PRSWIDDVSKLQQAKGLRTFLLFTKNPFFTRNPIEKVLLDRLFSHFPRLRVLQIPNVSKS 609
           PRSWIDDVSKLQQAKGLRTFLLFTKNPFFTRNPIEKVLLDRLFSHFPRLRVLQIPNVSKS
Sbjct: 181 PRSWIDDVSKLQQAKGLRTFLLFTKNPFFTRNPIEKVLLDRLFSHFPRLRVLQIPNVSKS 240

Query: 610 IKKLRHLRYLELGEDAKSVPNSITKLQNLQTLDLTKCYDLKELPRDINNFVNLRHLLCDS 669
           IKKLRHLRYLELGEDAKSVPNSITKLQNLQTLDLTKCYDLKELPRDINNFVNLRHLLCDS
Sbjct: 241 IKKLRHLRYLELGEDAKSVPNSITKLQNLQTLDLTKCYDLKELPRDINNFVNLRHLLCDS 300

Query: 670 RLMNMLQGTMEKLTSLQTLSSFLFDCKRFDKVKEFSERSYFIEFDLKIKGLEQLRFSPSD 729
           RLMNMLQGTMEKLTSLQTLSSFLFDCKRFDKVKEFSERSYFIEFDLKIKGLEQLRFSPSD
Sbjct: 301 RLMNMLQGTMEKLTSLQTLSSFLFDCKRFDKVKEFSERSYFIEFDLKIKGLEQLRFSPSD 360

Query: 730 VKSVNLKNKKVPLLRLKWKFENGNEYEGDADDIVLEGLEPHPYVNLLQIEGYCGVGLPNW 789
           VKSVNLKNKKVPLLRLKWKFENGNEYEGDADDIVLEGLEPHPYVNLLQIEGYCGVGLPNW
Sbjct: 361 VKSVNLKNKKVPLLRLKWKFENGNEYEGDADDIVLEGLEPHPYVNLLQIEGYCGVGLPNW 420

Query: 790 VSTSILLRGIRIGNCDRLHLNQLSHLHALEILNLEGLKSVMSISEWIGTLTSLVSLEIEE 849
           VSTSILLRGIRIGNCDRLHLNQLSHLHALEILNLEGLKSVMSISEWIGTLTSLVSLEIEE
Sbjct: 421 VSTSILLRGIRIGNCDRLHLNQLSHLHALEILNLEGLKSVMSISEWIGTLTSLVSLEIEE 480

Query: 850 CPKLKSLPKEMQQLKSLVQLNIIKCPQLGERCKEGGEDWPNISHIPDVLID 901
           CPKLKSLPKEMQQLKSLVQLNIIKCPQLGERCKEGGEDWPNISHIPDVLID
Sbjct: 481 CPKLKSLPKEMQQLKSLVQLNIIKCPQLGERCKEGGEDWPNISHIPDVLID 531

BLAST of Cucsa.128130 vs. NCBI nr
Match: gi|659122964|ref|XP_008461415.1| (PREDICTED: putative disease resistance protein RGA3 [Cucumis melo])

HSP 1 Score: 968.8 bits (2503), Expect = 6.7e-279
Identity = 516/718 (71.87%), Postives = 583/718 (81.20%), Query Frame = 1

Query: 191 NNLKKNLLAEDDKVKANVSFIAIVGMGGIGKTTLAKSLYNDKQVSDGFSSRIWIWVSNQF 250
           N  +K LLAEDDKVKANVSFIAIVGMGGIGKTTLAKSLYNDKQVSD F +RIWIWVS+QF
Sbjct: 99  NAKQKKLLAEDDKVKANVSFIAIVGMGGIGKTTLAKSLYNDKQVSDCFETRIWIWVSHQF 158

Query: 251 DTKTILKKIIESATEKKPKVEEMEPLKTKLEEVIGGKKYLLVMDDVWNENENEWENLKNL 310
           DTKTIL KIIESAT KKPKV+E+EPL TKL+EVIGGKKYLLVMDDVWNENENEWENLK+ 
Sbjct: 159 DTKTILGKIIESATVKKPKVDELEPLNTKLQEVIGGKKYLLVMDDVWNENENEWENLKSS 218

Query: 311 LMLGARGSKVLITKRDSKAVPGVETIPLKDLTEDFSWLLFKEVAFKESDLESINQNLIKM 370
           LM GARGSKVLITKRDSKA+  +ETIPLKDLTED SWLLFKE+AF+ESDLES NQNLIK+
Sbjct: 219 LMHGARGSKVLITKRDSKAISRIETIPLKDLTEDSSWLLFKEMAFEESDLESTNQNLIKL 278

Query: 371 GKEISKRCGGIPLVIRHIGRLLYGKTSAEDWEFIKENELLNVTREKNNNDGHVISTLKLS 430
           GKEISK+CGGIPLVIRHIGRLL GKTS E WEFIKEN+LLNVTRE+NNNDGHVISTLKLS
Sbjct: 279 GKEISKKCGGIPLVIRHIGRLLNGKTSVEYWEFIKENDLLNVTREENNNDGHVISTLKLS 338

Query: 431 YNHLSPNLKQCFSYSSLFPKGYKIRMNELIRQWIAQGFIESSNGGKSVENIGKEYLDELC 490
           YNHLSPNLKQCF+YSSLFPK Y+I   ELI QWIAQGFIESSN GKSVE+IGKEYL+ELC
Sbjct: 339 YNHLSPNLKQCFAYSSLFPKEYRITPTELIGQWIAQGFIESSNEGKSVEDIGKEYLNELC 398

Query: 491 WRFFYEISIEDVPF-----EEVGMHDLMCDLAREVAGQKLYIRGYPESGYVVSEQTRHIS 550
           WRFF+EI+     F     E   MHD+M DLAREVAG+KLY+RG P + YVVSEQTRHIS
Sbjct: 399 WRFFFEITSTSREFSFEDNELCCMHDVMRDLAREVAGKKLYVRGDPNNEYVVSEQTRHIS 458

Query: 551 FEYEPRSWIDDVSKLQQAKGLRTFLLFTKNPFFTRNPIEKVLLDRLFSHFPRLRVLQIPN 610
           FEY   SW D +SKL QAKGLRTFL   +      +PI   +LDRL S+FPRLRVLQI +
Sbjct: 459 FEYVIVSWKDVLSKLHQAKGLRTFLSLAE------SPITNGVLDRLVSNFPRLRVLQIFD 518

Query: 611 VSKSIKKLRHLRYLELG--EDAKSVPNSITKLQNLQTLDLTKCYDLKELPRDINNFVNLR 670
           VSKSIKKLRHLRYL+    +  KS+PN IT+   LQTLDLT C+    LPRDI N VNLR
Sbjct: 519 VSKSIKKLRHLRYLKFTRMDAGKSLPNCITE---LQTLDLTDCFSPVYLPRDIKNLVNLR 578

Query: 671 HLLCDSRLMNMLQGTMEKLTSLQTLSSFLFDCKRFDKVKEFSERSYFIEFDLKIKGLEQL 730
           +LL  S+ ++ ++  MEKLTSLQT+   L DCK+FDKVK+FS+ +  IE  L+I GLEQL
Sbjct: 579 YLLSYSKYIDAVE-IMEKLTSLQTIRYVLLDCKKFDKVKKFSKMNCSIESSLQIIGLEQL 638

Query: 731 RFSPSDVKSVNLKNKKVPLLRLKWKFENGNEYEGDADDIVLEGLEPHPYVNLLQIEGYCG 790
           RF  S VK VNLKNK+VP   +K +F+N N Y+GD D+ +LEG EPH  V  L IEGYCG
Sbjct: 639 RFFTSRVKLVNLKNKRVP--HVKLEFKNDNTYDGDDDETILEGFEPHRDVKCLDIEGYCG 698

Query: 791 VGLPNWVSTSILLRGIRIGNCDRL-HLNQLSHLHALEILNLEGLKSV-MSISEWIGTLTS 850
           VGLPNWVST  LL  + I NCDRL HLNQLSHL AL++L+L GLK V MSISEWI TLTS
Sbjct: 699 VGLPNWVSTLHLLTEVFIKNCDRLQHLNQLSHLQALKMLHLWGLKCVMMSISEWIVTLTS 758

Query: 851 LVSLEIEECPKLKSLPKEMQQLKSLVQLNIIKCPQLGERCKEGGEDWPNISHIPDVLI 900
           L  ++I  C KLKSLPKEMQQLK L +L I  CP+L ERCKEGG+DWPNISHIP++ +
Sbjct: 759 LEVMDIRNCWKLKSLPKEMQQLKCLRKLRIYGCPELKERCKEGGQDWPNISHIPELYL 804

BLAST of Cucsa.128130 vs. NCBI nr
Match: gi|659122964|ref|XP_008461415.1| (PREDICTED: putative disease resistance protein RGA3 [Cucumis melo])

HSP 1 Score: 141.0 bits (354), Expect = 1.0e-29
Identity = 73/93 (78.49%), Postives = 81/93 (87.10%), Query Frame = 1

Query: 22  IKKLSSHALECLGMVCGLNDDLNKLRSNVSSIQSVLRDAEQRQIKGNDHSLTDWLEKLGD 81
           +KKLSS A   LG  CG+ DDL+KLRSNV SIQ+VLR AEQ  ++GNDHSLTDWLEKLGD
Sbjct: 15  LKKLSSQASAYLGRRCGVKDDLDKLRSNVKSIQAVLRVAEQ--LQGNDHSLTDWLEKLGD 74

Query: 82  VFYDVEDVLDEISTEALRREVMTRGKNAKQVRI 115
           VFYDVEDVLDEISTEALRREVMTRGKNAKQ ++
Sbjct: 75  VFYDVEDVLDEISTEALRREVMTRGKNAKQKKL 105

HSP 2 Score: 850.1 bits (2195), Expect = 3.5e-243
Identity = 477/854 (55.85%), Postives = 608/854 (71.19%), Query Frame = 1

Query: 22  IKKLSSHALECLGMVCGLNDDLNKLRSNVSSIQSVLRDAEQRQIKGNDHSLTDWLEKLGD 81
           + KLSS A + LGM+ GL  DLNKL + VS+I+ VL DAE RQ K   H L +WL KL +
Sbjct: 14  LMKLSSQAFQRLGMLFGLKGDLNKLTTTVSTIKDVLLDAEGRQTKS--HLLQNWLHKLEE 73

Query: 82  VFYDVEDVLDEISTEALRREVMTRG-KNAKQVRIFFSNSNQLAFNYRMACQVKKINERLD 141
             YD EDVLDE+STEALRRE+MTR  KNAKQVRIFFS SNQ+AFNYRMA Q+K I ERLD
Sbjct: 74  ALYDAEDVLDELSTEALRRELMTRDHKNAKQVRIFFSKSNQIAFNYRMARQIKNIWERLD 133

Query: 142 VISQEKDKFQLNGIAYLGIQNVLSYPIGMERDTHSSLSGDQKIIGRDDEMNNLKKNLLAE 201
            I  EK +F L        Q      I M R+T SS S D+++IGRDD++  +K+ LL  
Sbjct: 134 AIDAEKTQFHLRENCESRTQYGSFDRIMMGRETWSS-SNDEEVIGRDDDIKEVKERLLDM 193

Query: 202 DDKVKANVSFIAIVGMGGIGKTTLAKSLYNDKQVSDGFSSRIWIWVSNQFDTKTILKKII 261
           +  V  NVSFIAI GMGGIGKTTLAKSLYND++VS  F  +IW+WVS+QF+ + + +K+I
Sbjct: 194 NMNVTHNVSFIAIAGMGGIGKTTLAKSLYNDEEVSGFFDLKIWVWVSDQFEVQVVAEKMI 253

Query: 262 ESATEKKPKVEEMEPLKTKLEEVIGGKKYLLVMDDVWNENENEWENLKNLLMLGARGSKV 321
           ESAT+  P V+ ME L+ KL++VIG +KYLLVMDDVWNE+E +W  LK+LLM GARGSKV
Sbjct: 254 ESATKNNPSVKGMEALQAKLQKVIGERKYLLVMDDVWNESEEKWHGLKSLLMGGARGSKV 313

Query: 322 LITKRDSKAVPGVETI----PLKDLTEDFSWLLFKEVAFKESDLESINQNLIKMGKEISK 381
           LITKRD K    ++++     L+ L+E  SWLLF +VAFKE   ES + + I +GKEI  
Sbjct: 314 LITKRDRKVATEIKSMTSLFTLEGLSESNSWLLFSKVAFKEGK-ESTDPSTIHLGKEILV 373

Query: 382 RCGGIPLVIRHIGRLLYGKTSAEDWEFIKENELLNVTREKNNNDGHVISTLKLSYNHLSP 441
           RCGG+PLVIRH+GR+LY KTS E+W   K+NELL V ++ N+    + S LKLSYNHL P
Sbjct: 374 RCGGVPLVIRHVGRMLYSKTSQEEWMSFKDNELLEVIQQDND----MTSILKLSYNHLPP 433

Query: 442 NLKQCFSYSSLFPKGYKIRMNELIRQWIAQGFIESSNGGKSVENIGKEYLDELCWRFFYE 501
           NLK+CF+YSSLFPKGYKI + +LIRQW+AQGFIE SNG KS+E+ GK+Y +ELCWRFFY 
Sbjct: 434 NLKRCFAYSSLFPKGYKIEIKDLIRQWVAQGFIEVSNGRKSLEDTGKDYFNELCWRFFYA 493

Query: 502 ISIEDVPFEE-VGMHDLMCDLAREVAGQKLYIRGYPESGYVVSEQTRHISFEYEPRSWID 561
            S ++    + V MHD+MC+  R+VAG KLY+RG P + YVVSEQT HISF+Y  +SW D
Sbjct: 494 NSSDECNINDIVCMHDVMCEFVRKVAGNKLYVRGNPNNDYVVSEQTLHISFDYGIQSWQD 553

Query: 562 DVSKLQQAKGLRTFLLFTKNPFFTRNPIEKVLLDRLFSHFPRLRVL-----QIPNVSKSI 621
            +SKL +AKGLRT LL  + P+   N I+K +LD LFS FPRLRVL     QI  V KSI
Sbjct: 554 VLSKLCKAKGLRTILLLFR-PYEKMNKIDKAILDELFSSFPRLRVLDLHFSQISVVPKSI 613

Query: 622 KKLRHLRYLELGE-DAKSVPNSITKLQNLQTLDLTKCYDLKELPRDINNFVNLRHLLCDS 681
           KKLRHLRYL+L E D + +P+SI +LQNLQTL+LT+CY+LKELPRDI+N VNLRHL  + 
Sbjct: 614 KKLRHLRYLDLSENDMELIPHSIIELQNLQTLNLTECYELKELPRDIDNLVNLRHLTFEP 673

Query: 682 RL-MNMLQGTMEKLTSLQTLSSFLFDCKRFDKVKEFSERSYFIEFDLKIKGLEQLRFSPS 741
            + +      MEKLT LQT+S F+FDCK+ +K+ E ++ SY +  +LKI GLE+LR SPS
Sbjct: 674 CMEVTPTSEGMEKLTCLQTISLFVFDCKKTNKLWELNDLSY-LTGELKIIGLEKLRSSPS 733

Query: 742 DVKSVNLKNKK-VPLLRLKWKFENGNEYEGDADDIVLEGLEPHPYVNLLQIEGYCGVGLP 801
           ++  +NLK+KK    L L+WK    +EYEG+AD+ ++EGLEPHP V  L I GY G  LP
Sbjct: 734 EITLINLKDKKGWQGLNLEWKL-GKDEYEGEADETIMEGLEPHPNVESLSINGYTGGALP 793

Query: 802 NWVSTSIL-LRGIRIGNCDRL-HLNQLSHLHALEILNLEGLKSVMSISE---WIGTL--T 855
           NWV  S++ L  I I NC R+ HL Q + L  L  L+L GL+S+  I +   +  ++   
Sbjct: 794 NWVFNSLMKLTEIEIENCPRVQHLPQFNQLQDLRALHLVGLRSLEFIDKSDPYSSSVFFP 853