Cucsa.128100 (gene) Cucumber (Gy14) v1

NameCucsa.128100
Typegene
OrganismCucumis sativus (Cucumber (Gy14) v1)
DescriptionNBS-LRR type disease resistance protein
Locationscaffold01024 : 218668 .. 222099 (+)
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCGGATTCAGTTCTGTTCAATGTTGCTGCAAGTGTTATTACTAAACTGGGATCTTCCGCACTTCGAGAACTTGGGTCTCTGTGGGGAGTCAACGATGAGCTCGACAAACTCCAAAACACTCTTTCGGCCATTAAAGCCGTCCTTCTCGATGCAGAGGAGCAACAGTCCAAGAGCCACACAGTCAAGGATTGGATTGCAAAGATCAAAGATGTTTTCTATGACATTGATGACTTGATTGACGAGTTCTCTTATGAAACCTTGAGAAGACAAGTTCTTACCAAGGATAGAACAATCACCAAACAAGTACGTATCTTCTTCTCCAAATCTAATCAGATTGCTTTTGGTTTCAAAATGGGTCAAACAATTAAAAAAGTTAGGGAGAAGCTAGATGCTATTGCGGCTATTAAAGCTCAACTTCACCTCTCTGTGTGTGCGAGGGAGGTACGAGATAATGAGCCAAGGAAGGTACGAGAGACGTCCTCATTCATACCCGAGGGGGAAATCATTGGTAGGGATGAGGATAGGAAATCTGTTATGGATTTTCTATTGAATACCAGCAACATCACAAAGGATAACGTTGAAGTTGTTTCCATTGTTGGAATGGGAGGATTAGGAAAGACAGCACTCGCTCAAACTGTCTATAATGATGAAAAAATAAaCAATCGTTTTAAGTGGAAAATATGGGTGTGTATTTCTCAAGAATTTGATATCAAAGTAATTGTTGAAAAGATTTTAGAGTCTATTACGAAAACAAAACAAGAATCCCTTCAGTTGGATATATTACAAAGTATGCTTCAAGAGAAAATTTATGGAAAAAAaTACTTGTTGGTCATGGATGATGTGTGGAATGTAGACCACGAGAAATGGATTGGTCTGAAAAGATTTCTGATGGGTGGTGCCAGCGGAAGTAAGATTTTGGTGACAACCCGTAATCTACAAACTGCCCAGGCTTCTGACACGGTTTGGTTCCATCACTTAAAAGAACTTGACAAGGATAACTCTTGGGCGTTGTTTAGGAAAATGGCATTCTTAAACAAAGAAGAAGAGCTTGAGAATTCAAATTTGGTTAGAATCGGTAAAGAGATTGTAGCAAAGTTGAAAGGTTATCCCCTTTCAATAAGAGTTGTTGGACGTCTGTTATATTTCAAAAACACAGAAATGGATTGGTCATCATTTAAGGACAACGAACTTGACTCAATTTTGCAAGAAGATGATCAAATTCAACCAATACTGAAGATAAGTTTTAACCACCTTCCACCTAAATTGAAGCAATGTTTTACGTACTGTGCTTTGTTTCCCAAGGATTATGAGTTTAAAAAGAATGGATTGGTAAAACAATGGATGGCACAAGGTTTCATTCAAGCACATAATAAAAAGGCAATTGAAGATGTGGGTGACGATTATTTTCAAGAGTTAGTGGGGAGGTCATTCTTTCAAGACATAAGAAAAAaCAAATGGGGAGACTTAAAGTACTGTAAGATGCATGATTTGTTACATGATCTTGCGTGTTCGATAGGAGAAAATGAATGTGTGGTTGTAAGTGATGATGTCGGGTCCATTGACAAAAGGACTCGACATGCCTCATTTCTCTTGAGCAAGAGGCTAACAAGGGAAGTTGTATCAAAATCATCCATTGAGGTAACGAGTTTGAGAACATTGGATATTGATAGTCGTGCTTCTTTCCGTTCTTTCAAGAAAACTTGTCACATGAACCTTTTTCAATTACGAACATTGAATTTGGATAGATGCTGCTGTCATCCTCCTAAGTTTGTTGATAAGTTGAAACATTTGAGATATCTTAATCTTTCTGGTTTAAATGTAACTTTCCTTCCCAATTCTATTACCACATTGTATAATTTGGAAACACTTATCCTTCGTTACTGCCTTTGGCTAAGAAAATTGCCAAAAGATATTAACAATTTGATCAATCTCAGGCATCTTGATATTTATGATTGTTCCAGTTTGACTCACATGCCAAAAGGATTAGGTGGGATGACTAGCCTTCAGACAATGAGTATGTTTGTATTAGGAAAGAATAAAGGTGGTGATTTAAGTGCATTGAATGGACTTAAAAGCTTGAGAGGATTATTATGTATTAAAGGTTTACAATTTTGCACAACTGCTGATTTAAAAAATGTAAGCTACTTAAAAGAAATGTATGGAATTCAAAAGCTGGAATTACACTGGGATATAAAAATGGATCATGAAGATGCCTTAGATGATGGTGATAATGATGATGAGGGAGTTTTGGAGGGCTTAAAACCaCATTCAAATATTCGCAAAATGATTATAAAAGGATACAGAGGAATGAAGTTATGTGATTGGTTTTCTTCTAATTTCCTGGGTGGTTTGGTTAGCATAGAGCTTTCACATTGTGAAAAATTGGAGCATCTCCCACAGTTTGATCAATTCCTATATCTCAAGCATCTTCTTCTTGGATACTTACCCAATATTGAATACATTGATAGCGGCAATTCTGTTTCTTCATCAACAACATTTTTTCCATCTCTCGAGAAGCTAAGGATTGAGAGCATGCCTAAGTTGAAAGGgTGGTGgAAGGGGgAAaTTTCATTTCCAACAACAATATTACATCAACTCTCAGAATTATGTATTTTTtATTGTCCTCTGTTGGCTTCTATTCCACAACATCCATCTTTGGAATCATTGAGAATATGTGGTGTTAGTGTGCAACTTTTTCAAATGGTAATACGAATGGCTACAGACCTTTCTGAACATTCTTCTTCTTCTTCAACATTGTCTAAATTATCTTTCCTTGAGATTGGAACTATTGATCTTGAGTTCTTGCCAGTGGAGTTATTCTGCAATATGACACATCTTGAGTCTCTTATCATAGAACGCTGCAAAAGTTTACAAATGTCTTCTCCGCATCCTGTTGATGAGGATAATGATGTGGTATGGAAAAAACTCAGCAATCTCCGGACACTTCGGCTTGAGAGCATCCTCAAATTGGAGTATTTTCCCAAGAGTTTGAAATATATTACAAGTCTTGAAACTTTGAAGCTATCAAATTGTGAAAATTTAGTGAGTACGGAAGGGATTGGCGAACTCATTTCACTATCACATTTGGAAATTGATAGATGTCCTAATTTACCTATATTGTCGGAAGATGTCGGCGACCTCATTTCCCTATCACACTTGCTTATTTGGAATTGTCCCAAATTAACTTCCTTGTCAGAAGGAATCACTCGCCTCACTTCACTCTCAAGTTTGTGTCTTGAAGATTGTCCCAACTTAGTCTCCTTGCCCCAAGAATTTCTCCACCACCACAGCTCCTTACCAGGAGGACGGTTCTTGAGAATTTTGAACTGTCCCAAATTGCAGATTCAaGACAAGAAACAAAAGGAAGAAGAAGAAGAAGACCAGGAGGATTGGAATGAACTCATCCATGTATTGACCGGATGTAGGTAA

mRNA sequence

ATGGCGGATTCAGTTCTGTTCAATGTTGCTGCAAGTGTTATTACTAAACTGGGATCTTCCGCACTTCGAGAACTTGGGTCTCTGTGGGGAGTCAACGATGAGCTCGACAAACTCCAAAACACTCTTTCGGCCATTAAAGCCGTCCTTCTCGATGCAGAGGAGCAACAGTCCAAGAGCCACACAGTCAAGGATTGGATTGCAAAGATCAAAGATGTTTTCTATGACATTGATGACTTGATTGACGAGTTCTCTTATGAAACCTTGAGAAGACAAGTTCTTACCAAGGATAGAACAATCACCAAACAAGTACGTATCTTCTTCTCCAAATCTAATCAGATTGCTTTTGGTTTCAAAATGGGTCAAACAATTAAAAAAGTTAGGGAGAAGCTAGATGCTATTGCGGCTATTAAAGCTCAACTTCACCTCTCTGTGTGTGCGAGGGAGGTACGAGATAATGAGCCAAGGAAGGTACGAGAGACGTCCTCATTCATACCCGAGGGGGAAATCATTGGTAGGGATGAGGATAGGAAATCTGTTATGGATTTTCTATTGAATACCAGCAACATCACAAAGGATAACGTTGAAGTTGTTTCCATTGTTGGAATGGGAGGATTAGGAAAGACAGCACTCGCTCAAACTGTCTATAATGATGAAAAAATAAACAATCGTTTTAAGTGGAAAATATGGGTGTGTATTTCTCAAGAATTTGATATCAAAGTAATTGTTGAAAAGATTTTAGAGTCTATTACGAAAACAAAACAAGAATCCCTTCAGTTGGATATATTACAAAGTATGCTTCAAGAGAAAATTTATGGAAAAAAATACTTGTTGGTCATGGATGATGTGTGGAATGTAGACCACGAGAAATGGATTGGTCTGAAAAGATTTCTGATGGGTGGTGCCAGCGGAAGTAAGATTTTGGTGACAACCCGTAATCTACAAACTGCCCAGGCTTCTGACACGGTTTGGTTCCATCACTTAAAAGAACTTGACAAGGATAACTCTTGGGCGTTGTTTAGGAAAATGGCATTCTTAAACAAAGAAGAAGAGCTTGAGAATTCAAATTTGGTTAGAATCGGTAAAGAGATTGTAGCAAAGTTGAAAGGTTATCCCCTTTCAATAAGAGTTGTTGGACGTCTGTTATATTTCAAAAACACAGAAATGGATTGGTCATCATTTAAGGACAACGAACTTGACTCAATTTTGCAAGAAGATGATCAAATTCAACCAATACTGAAGATAAGTTTTAACCACCTTCCACCTAAATTGAAGCAATGTTTTACGTACTGTGCTTTGTTTCCCAAGGATTATGAGTTTAAAAAGAATGGATTGGTAAAACAATGGATGGCACAAGGTTTCATTCAAGCACATAATAAAAAGGCAATTGAAGATGTGGGTGACGATTATTTTCAAGAGTTAGTGGGGAGGTCATTCTTTCAAGACATAAGAAAAAACAAATGGGGAGACTTAAAGTACTGTAAGATGCATGATTTGTTACATGATCTTGCGTGTTCGATAGGAGAAAATGAATGTGTGGTTGTAAGTGATGATGTCGGGTCCATTGACAAAAGGACTCGACATGCCTCATTTCTCTTGAGCAAGAGGCTAACAAGGGAAGTTGTATCAAAATCATCCATTGAGGTAACGAGTTTGAGAACATTGGATATTGATAGTCGTGCTTCTTTCCGTTCTTTCAAGAAAACTTGTCACATGAACCTTTTTCAATTACGAACATTGAATTTGGATAGATGCTGCTGTCATCCTCCTAAGTTTGTTGATAAGTTGAAACATTTGAGATATCTTAATCTTTCTGGTTTAAATGTAACTTTCCTTCCCAATTCTATTACCACATTGTATAATTTGGAAACACTTATCCTTCGTTACTGCCTTTGGCTAAGAAAATTGCCAAAAGATATTAACAATTTGATCAATCTCAGGCATCTTGATATTTATGATTGTTCCAGTTTGACTCACATGCCAAAAGGATTAGGTGGGATGACTAGCCTTCAGACAATGAGTATGTTTGTATTAGGAAAGAATAAAGGTGGTGATTTAAGTGCATTGAATGGACTTAAAAGCTTGAGAGGATTATTATGTATTAAAGGTTTACAATTTTGCACAACTGCTGATTTAAAAAATCTGGAATTACACTGGGATATAAAAATGGATCATGAAGATGCCTTAGATGATGGTGATAATGATGATGAGGGAGTTTTGGAGGGCTTAAAACCACATTCAAATATTCGCAAAATGATTATAAAAGGATACAGAGGAATGAAGTTATGTGATTGGTTTTCTTCTAATTTCCTGGGTGGTTTGGTTAGCATAGAGCTTTCACATTGTGAAAAATTGGAGCATCTCCCACAGTTTGATCAATTCCTATATCTCAAGCATCTTCTTCTTGGATACTTACCCAATATTGAATACATTGATAGCGGCAATTCTGTTTCTTCATCAACAACATTTTTTCCATCTCTCGAGAAGCTAAGGATTGAGAGCATGCCTAAGTTGAAAGGGTGGTGGAAGGGGGAAATTTCATTTCCAACAACAATATTACATCAACTCTCAGAATTATGTATTTTTTATTGTCCTCTGTTGGCTTCTATTCCACAACATCCATCTTTGGAATCATTGAGAATATGTGGTGTTAGTGTGCAACTTTTTCAAATGGTAATACGAATGGCTACAGACCTTTCTGAACATTCTTCTTCTTCTTCAACATTGTCTAAATTATCTTTCCTTGAGATTGGAACTATTGATCTTGAGTTCTTGCCAGTGGAGTTATTCTGCAATATGACACATCTTGAGTCTCTTATCATAGAACGCTGCAAAAGTTTACAAATGTCTTCTCCGCATCCTGTTGATGAGGATAATGATGTGGTATGGAAAAAACTCAGCAATCTCCGGACACTTCGGCTTGAGAGCATCCTCAAATTGGAGTATTTTCCCAAGAGTTTGAAATATATTACAAGTCTTGAAACTTTGAAGCTATCAAATTGTGAAAATTTAGTGAGTACGGAAGGGATTGGCGAACTCATTTCACTATCACATTTGGAAATTGATAGATGTCCTAATTTACCTATATTGTCGGAAGATGTCGGCGACCTCATTTCCCTATCACACTTGCTTATTTGGAATTGTCCCAAATTAACTTCCTTGTCAGAAGGAATCACTCGCCTCACTTCACTCTCAAGTTTGTGTCTTGAAGATTGTCCCAACTTAGTCTCCTTGCCCCAAGAATTTCTCCACCACCACAGCTCCTTACCAGGAGGACGGTTCTTGAGAATTTTGAACTGTCCCAAATTGCAGATTCAAGACAAGAAACAAAAGGAAGAAGAAGAAGAAGACCAGGAGGATTGGAATGAACTCATCCATGTATTGACCGGATGTAGGTAA

Coding sequence (CDS)

ATGGCGGATTCAGTTCTGTTCAATGTTGCTGCAAGTGTTATTACTAAACTGGGATCTTCCGCACTTCGAGAACTTGGGTCTCTGTGGGGAGTCAACGATGAGCTCGACAAACTCCAAAACACTCTTTCGGCCATTAAAGCCGTCCTTCTCGATGCAGAGGAGCAACAGTCCAAGAGCCACACAGTCAAGGATTGGATTGCAAAGATCAAAGATGTTTTCTATGACATTGATGACTTGATTGACGAGTTCTCTTATGAAACCTTGAGAAGACAAGTTCTTACCAAGGATAGAACAATCACCAAACAAGTACGTATCTTCTTCTCCAAATCTAATCAGATTGCTTTTGGTTTCAAAATGGGTCAAACAATTAAAAAAGTTAGGGAGAAGCTAGATGCTATTGCGGCTATTAAAGCTCAACTTCACCTCTCTGTGTGTGCGAGGGAGGTACGAGATAATGAGCCAAGGAAGGTACGAGAGACGTCCTCATTCATACCCGAGGGGGAAATCATTGGTAGGGATGAGGATAGGAAATCTGTTATGGATTTTCTATTGAATACCAGCAACATCACAAAGGATAACGTTGAAGTTGTTTCCATTGTTGGAATGGGAGGATTAGGAAAGACAGCACTCGCTCAAACTGTCTATAATGATGAAAAAATAAaCAATCGTTTTAAGTGGAAAATATGGGTGTGTATTTCTCAAGAATTTGATATCAAAGTAATTGTTGAAAAGATTTTAGAGTCTATTACGAAAACAAAACAAGAATCCCTTCAGTTGGATATATTACAAAGTATGCTTCAAGAGAAAATTTATGGAAAAAAaTACTTGTTGGTCATGGATGATGTGTGGAATGTAGACCACGAGAAATGGATTGGTCTGAAAAGATTTCTGATGGGTGGTGCCAGCGGAAGTAAGATTTTGGTGACAACCCGTAATCTACAAACTGCCCAGGCTTCTGACACGGTTTGGTTCCATCACTTAAAAGAACTTGACAAGGATAACTCTTGGGCGTTGTTTAGGAAAATGGCATTCTTAAACAAAGAAGAAGAGCTTGAGAATTCAAATTTGGTTAGAATCGGTAAAGAGATTGTAGCAAAGTTGAAAGGTTATCCCCTTTCAATAAGAGTTGTTGGACGTCTGTTATATTTCAAAAACACAGAAATGGATTGGTCATCATTTAAGGACAACGAACTTGACTCAATTTTGCAAGAAGATGATCAAATTCAACCAATACTGAAGATAAGTTTTAACCACCTTCCACCTAAATTGAAGCAATGTTTTACGTACTGTGCTTTGTTTCCCAAGGATTATGAGTTTAAAAAGAATGGATTGGTAAAACAATGGATGGCACAAGGTTTCATTCAAGCACATAATAAAAAGGCAATTGAAGATGTGGGTGACGATTATTTTCAAGAGTTAGTGGGGAGGTCATTCTTTCAAGACATAAGAAAAAaCAAATGGGGAGACTTAAAGTACTGTAAGATGCATGATTTGTTACATGATCTTGCGTGTTCGATAGGAGAAAATGAATGTGTGGTTGTAAGTGATGATGTCGGGTCCATTGACAAAAGGACTCGACATGCCTCATTTCTCTTGAGCAAGAGGCTAACAAGGGAAGTTGTATCAAAATCATCCATTGAGGTAACGAGTTTGAGAACATTGGATATTGATAGTCGTGCTTCTTTCCGTTCTTTCAAGAAAACTTGTCACATGAACCTTTTTCAATTACGAACATTGAATTTGGATAGATGCTGCTGTCATCCTCCTAAGTTTGTTGATAAGTTGAAACATTTGAGATATCTTAATCTTTCTGGTTTAAATGTAACTTTCCTTCCCAATTCTATTACCACATTGTATAATTTGGAAACACTTATCCTTCGTTACTGCCTTTGGCTAAGAAAATTGCCAAAAGATATTAACAATTTGATCAATCTCAGGCATCTTGATATTTATGATTGTTCCAGTTTGACTCACATGCCAAAAGGATTAGGTGGGATGACTAGCCTTCAGACAATGAGTATGTTTGTATTAGGAAAGAATAAAGGTGGTGATTTAAGTGCATTGAATGGACTTAAAAGCTTGAGAGGATTATTATGTATTAAAGGTTTACAATTTTGCACAACTGCTGATTTAAAAAATCTGGAATTACACTGGGATATAAAAATGGATCATGAAGATGCCTTAGATGATGGTGATAATGATGATGAGGGAGTTTTGGAGGGCTTAAAACCaCATTCAAATATTCGCAAAATGATTATAAAAGGATACAGAGGAATGAAGTTATGTGATTGGTTTTCTTCTAATTTCCTGGGTGGTTTGGTTAGCATAGAGCTTTCACATTGTGAAAAATTGGAGCATCTCCCACAGTTTGATCAATTCCTATATCTCAAGCATCTTCTTCTTGGATACTTACCCAATATTGAATACATTGATAGCGGCAATTCTGTTTCTTCATCAACAACATTTTTTCCATCTCTCGAGAAGCTAAGGATTGAGAGCATGCCTAAGTTGAAAGGgTGGTGgAAGGGGgAAaTTTCATTTCCAACAACAATATTACATCAACTCTCAGAATTATGTATTTTTtATTGTCCTCTGTTGGCTTCTATTCCACAACATCCATCTTTGGAATCATTGAGAATATGTGGTGTTAGTGTGCAACTTTTTCAAATGGTAATACGAATGGCTACAGACCTTTCTGAACATTCTTCTTCTTCTTCAACATTGTCTAAATTATCTTTCCTTGAGATTGGAACTATTGATCTTGAGTTCTTGCCAGTGGAGTTATTCTGCAATATGACACATCTTGAGTCTCTTATCATAGAACGCTGCAAAAGTTTACAAATGTCTTCTCCGCATCCTGTTGATGAGGATAATGATGTGGTATGGAAAAAACTCAGCAATCTCCGGACACTTCGGCTTGAGAGCATCCTCAAATTGGAGTATTTTCCCAAGAGTTTGAAATATATTACAAGTCTTGAAACTTTGAAGCTATCAAATTGTGAAAATTTAGTGAGTACGGAAGGGATTGGCGAACTCATTTCACTATCACATTTGGAAATTGATAGATGTCCTAATTTACCTATATTGTCGGAAGATGTCGGCGACCTCATTTCCCTATCACACTTGCTTATTTGGAATTGTCCCAAATTAACTTCCTTGTCAGAAGGAATCACTCGCCTCACTTCACTCTCAAGTTTGTGTCTTGAAGATTGTCCCAACTTAGTCTCCTTGCCCCAAGAATTTCTCCACCACCACAGCTCCTTACCAGGAGGACGGTTCTTGAGAATTTTGAACTGTCCCAAATTGCAGATTCAaGACAAGAAACAAAAGGAAGAAGAAGAAGAAGACCAGGAGGATTGGAATGAACTCATCCATGTATTGACCGGATGTAGGTAA

Protein sequence

MADSVLFNVAASVITKLGSSALRELGSLWGVNDELDKLQNTLSAIKAVLLDAEEQQSKSHTVKDWIAKIKDVFYDIDDLIDEFSYETLRRQVLTKDRTITKQVRIFFSKSNQIAFGFKMGQTIKKVREKLDAIAAIKAQLHLSVCAREVRDNEPRKVRETSSFIPEGEIIGRDEDRKSVMDFLLNTSNITKDNVEVVSIVGMGGLGKTALAQTVYNDEKINNRFKWKIWVCISQEFDIKVIVEKILESITKTKQESLQLDILQSMLQEKIYGKKYLLVMDDVWNVDHEKWIGLKRFLMGGASGSKILVTTRNLQTAQASDTVWFHHLKELDKDNSWALFRKMAFLNKEEELENSNLVRIGKEIVAKLKGYPLSIRVVGRLLYFKNTEMDWSSFKDNELDSILQEDDQIQPILKISFNHLPPKLKQCFTYCALFPKDYEFKKNGLVKQWMAQGFIQAHNKKAIEDVGDDYFQELVGRSFFQDIRKNKWGDLKYCKMHDLLHDLACSIGENECVVVSDDVGSIDKRTRHASFLLSKRLTREVVSKSSIEVTSLRTLDIDSRASFRSFKKTCHMNLFQLRTLNLDRCCCHPPKFVDKLKHLRYLNLSGLNVTFLPNSITTLYNLETLILRYCLWLRKLPKDINNLINLRHLDIYDCSSLTHMPKGLGGMTSLQTMSMFVLGKNKGGDLSALNGLKSLRGLLCIKGLQFCTTADLKNLELHWDIKMDHEDALDDGDNDDEGVLEGLKPHSNIRKMIIKGYRGMKLCDWFSSNFLGGLVSIELSHCEKLEHLPQFDQFLYLKHLLLGYLPNIEYIDSGNSVSSSTTFFPSLEKLRIESMPKLKGWWKGEISFPTTILHQLSELCIFYCPLLASIPQHPSLESLRICGVSVQLFQMVIRMATDLSEHSSSSSTLSKLSFLEIGTIDLEFLPVELFCNMTHLESLIIERCKSLQMSSPHPVDEDNDVVWKKLSNLRTLRLESILKLEYFPKSLKYITSLETLKLSNCENLVSTEGIGELISLSHLEIDRCPNLPILSEDVGDLISLSHLLIWNCPKLTSLSEGITRLTSLSSLCLEDCPNLVSLPQEFLHHHSSLPGGRFLRILNCPKLQIQDKKQKEEEEEDQEDWNELIHVLTGCR*
BLAST of Cucsa.128100 vs. Swiss-Prot
Match: RGA4_SOLBU (Putative disease resistance protein RGA4 OS=Solanum bulbocastanum GN=RGA4 PE=2 SV=1)

HSP 1 Score: 501.1 bits (1289), Expect = 3.1e-140
Identity = 349/1015 (34.38%), Postives = 522/1015 (51.43%), Query Frame = 1

Query: 28   LWGVNDELDKLQNTLSAIKAVLLDAEEQQSKSHTVKDWIAKIKDVFYDIDDLIDEFSYET 87
            ++G   E +KL +  S I+AVL DA+E+Q K   +++W+ K+    Y++DD++ E   E 
Sbjct: 24   IFGFEKECEKLSSVFSTIQAVLQDAQEKQLKDKAIENWLQKLNSAAYEVDDILGECKNEA 83

Query: 88   LRRQVLTKDRTITKQVRIFFSKSNQIAFGFKMGQTIKKVREKLDAIAAIKAQLHLSVCAR 147
            +R +          Q R+ F     I F  K+G+ +K++ EKLDAI+  + + H     +
Sbjct: 84   IRFE----------QSRLGFYHPGIINFRHKIGRRMKEIMEKLDAISEERRKFHF--LEK 143

Query: 148  EVRDNEPRKVRETSSFIPEGEIIGRDEDRKSVMDFLLNTSNITKDNVEVVSIVGMGGLGK 207
                      RET   + E ++ GRD++   ++  L+N  N+ ++ + V  I+GMGGLGK
Sbjct: 144  ITERQAAAATRETGFVLTEPKVYGRDKEEDEIVKILINNVNVAEE-LPVFPIIGMGGLGK 203

Query: 208  TALAQTVYNDEKINNRFKWKIWVCISQEFDIKVIVEKILESITKTKQESLQLDILQSMLQ 267
            T LAQ ++NDE++   F  KIWVC+S +FD K +++ I+ +I ++      L   Q  LQ
Sbjct: 204  TTLAQMIFNDERVTKHFNPKIWVCVSDDFDEKRLIKTIIGNIERSSPHVEDLASFQKKLQ 263

Query: 268  EKIYGKKYLLVMDDVWNVDHEKWIGLKRFLMGGASGSKILVTTRNLQTAQASDTVWFHHL 327
            E + GK+YLLV+DDVWN D EKW  L+  L  GA G+ IL TTR  +      T+  +HL
Sbjct: 264  ELLNGKRYLLVLDDVWNDDLEKWAKLRAVLTVGARGASILATTRLEKVGSIMGTLQPYHL 323

Query: 328  KELDKDNSWALFRKMAFLNKEEELENSNLVRIGKEIVAKLKGYPLSIRVVGRLLYFKNTE 387
              L   +S  LF + AF  ++E   N NLV IGKEIV K  G PL+ + +G LL FK  E
Sbjct: 324  SNLSPHDSLLLFMQRAFGQQKEA--NPNLVAIGKEIVKKCGGVPLAAKTLGGLLRFKREE 383

Query: 388  MDWSSFKDNELDSILQEDDQIQPILKISFNHLPPKLKQCFTYCALFPKDYEFKKNGLVKQ 447
             +W   +DNE+ S+ Q++  I P L++S++HLP  L+QCF YCA+FPKD +  K  L+  
Sbjct: 384  SEWEHVRDNEIWSLPQDESSILPALRLSYHHLPLDLRQCFAYCAVFPKDTKMIKENLITL 443

Query: 448  WMAQGFIQAHNKKAIEDVGDDYFQELVGRSFFQDIRKNKWGDLKYCKMHDLLHDLACSIG 507
            WMA GF+ +     +EDVG++ + EL  RSFFQ+I + K G+  Y K+HDL+HDLA S+ 
Sbjct: 444  WMAHGFLLSKGNLELEDVGNEVWNELYLRSFFQEI-EAKSGN-TYFKIHDLIHDLATSL- 503

Query: 508  ENECVVVSDDVGSIDKRTRHASFLLSKRLTREVVSKSSIEVTSLRTLDIDSRASFRSFKK 567
                   S   G+I                RE+  K      S+    + S  S    KK
Sbjct: 504  ----FSASASCGNI----------------REINVKDYKHTVSIGFAAVVSSYSPSLLKK 563

Query: 568  TCHMNLFQLRTLNLDRCCCHPPKFVDKLKHLRYLNLSGLNVTFLPNSITTLYNLETLILR 627
               + +  L    L++     P  +  L HLRYL+LS  N   LP  +  L NL+TL + 
Sbjct: 564  FVSLRVLNLSYSKLEQL----PSSIGDLLHLRYLDLSCNNFRSLPERLCKLQNLQTLDVH 623

Query: 628  YCLWLRKLPKDINNLINLRHLDIYDCSSLTHMPKGLGGMTSLQTMSMFVLGKNKGGDLSA 687
             C  L  LPK  + L +LRHL +  C  LT  P  +G +T L+T+  F++G  KG  L  
Sbjct: 624  NCYSLNCLPKQTSKLSSLRHLVVDGC-PLTSTPPRIGLLTCLKTLGFFIVGSKKGYQLGE 683

Query: 688  LNGLKSLRGLLCIKGLQFCTTADLKNLELHWDIKMDHEDALDDGDND--------DEGVL 747
            L  L +L G + I  L+     +  + E +   K + +      DND        +  VL
Sbjct: 684  LKNL-NLCGSISITHLE--RVKNDTDAEANLSAKANLQSLSMSWDNDGPNRYESKEVKVL 743

Query: 748  EGLKPHSNIRKMIIKGYRGMKLCDWFSSNFLGGLVSIELSHCEKLEHLPQFDQFLYLKHL 807
            E LKPH N++ + I  + G +   W + + L  ++S+ +  C+    LP F +   L++L
Sbjct: 744  EALKPHPNLKYLEIIAFGGFRFPSWINHSVLEKVISVRIKSCKNCLCLPPFGELPCLENL 803

Query: 808  -LLGYLPNIEYI--DSGNSVSSSTTFFPSLEKLRIESMPKLKGWWK--GEISFPTTILHQ 867
             L      +EY+  D  +S  S+   FPSL+KLRI     LKG  K  GE  FP      
Sbjct: 804  ELQNGSAEVEYVEEDDVHSRFSTRRSFPSLKKLRIWFFRSLKGLMKEEGEEKFP-----M 863

Query: 868  LSELCIFYCPLLASIPQHPSLESLRICGVSVQLFQMVIRMATDLSEHSSSSSTLSKLSFL 927
            L E+ I YCPL    P   S++ L + G                +   SS S LS L+ L
Sbjct: 864  LEEMAILYCPLFV-FPTLSSVKKLEVHG-------------NTNTRGLSSISNLSTLTSL 923

Query: 928  EIG-TIDLEFLPVELFCNMTHLESLIIERCKSLQMSSPHPVDEDNDVVWKKLSNLRTLRL 987
             IG       LP E+F ++T+LE L     K+L         +D       L+ L+ L++
Sbjct: 924  RIGANYRATSLPEEMFTSLTNLEFLSFFDFKNL---------KDLPTSLTSLNALKRLQI 964

Query: 988  ESILKLEYFP-KSLKYITSLETLKLSNCENL-VSTEGIGELISLSHLEIDRCPNL 1027
            ES   LE FP + L+ +TSL  L +  C+ L    EG+  L +L++L +  CP +
Sbjct: 984  ESCDSLESFPEQGLEGLTSLTQLFVKYCKMLKCLPEGLQHLTALTNLGVSGCPEV 964


HSP 2 Score: 49.3 bits (116), Expect = 3.2e-04
Identity = 41/105 (39.05%), Postives = 54/105 (51.43%), Query Frame = 1

Query: 963  KKLSNLRTLRLESILKLEYFPKSLKYITSLETLKLSNCENLVST-EGIGELISLSHLEID 1022
            KK  +LR L L S  KLE  P S+  +  L  L LS C N  S  E + +L +L  L++ 
Sbjct: 524  KKFVSLRVLNL-SYSKLEQLPSSIGDLLHLRYLDLS-CNNFRSLPERLCKLQNLQTLDVH 583

Query: 1023 RCPNLPILSEDVGDLISLSHLLIWNCPKLTSLSEGITRLTSLSSL 1067
             C +L  L +    L SL HL++  CP LTS    I  LT L +L
Sbjct: 584  NCYSLNCLPKQTSKLSSLRHLVVDGCP-LTSTPPRIGLLTCLKTL 625


HSP 3 Score: 47.4 bits (111), Expect = 1.2e-03
Identity = 34/113 (30.09%), Postives = 58/113 (51.33%), Query Frame = 1

Query: 965  LSNLRTLRLESILKLEYFPKSL-KYITSLETLKLSNCENLVSTE-GIGELISLSHLEIDR 1024
            LS L +LR+ +  +    P+ +   +T+LE L   + +NL      +  L +L  L+I+ 
Sbjct: 852  LSTLTSLRIGANYRATSLPEEMFTSLTNLEFLSFFDFKNLKDLPTSLTSLNALKRLQIES 911

Query: 1025 CPNLPILSED-VGDLISLSHLLIWNCPKLTSLSEGITRLTSLSSLCLEDCPNL 1075
            C +L    E  +  L SL+ L +  C  L  L EG+  LT+L++L +  CP +
Sbjct: 912  CDSLESFPEQGLEGLTSLTQLFVKYCKMLKCLPEGLQHLTALTNLGVSGCPEV 964

BLAST of Cucsa.128100 vs. Swiss-Prot
Match: RGA1_SOLBU (Putative disease resistance protein RGA1 OS=Solanum bulbocastanum GN=RGA1 PE=2 SV=2)

HSP 1 Score: 499.2 bits (1284), Expect = 1.2e-139
Identity = 353/1028 (34.34%), Postives = 536/1028 (52.14%), Query Frame = 1

Query: 13   VITKLGSSALRELGSLWGVNDELDKLQNTLSAIKAVLLDAEEQQSKSHTVKDWIAKIKDV 72
            V+  L S    EL  L+G  DE  +L +  S I+AVL DA+E+Q     +++W+ K+   
Sbjct: 9    VLDNLTSFLKGELVLLFGFQDEFQRLSSMFSTIQAVLEDAQEKQLNDKPLENWLQKLNAA 68

Query: 73   FYDIDDLIDEFSYETLRRQVLTKDRTITKQVRIFFSKSNQ-----IAFGFKMGQTIKKVR 132
             Y++DD++DE+                TK  R   S+  +     I F  K+G+ + +V 
Sbjct: 69   TYEVDDILDEYK---------------TKATRFLQSEYGRYHPKVIPFRHKVGKRMDQVM 128

Query: 133  EKLDAIAAIKAQLHLSVCAREVRDNEPRKVRETSSFIPEGEIIGRDEDRKSVMDFLLNTS 192
            +KL+AIA  + + HL    +E         RET S + E ++ GRD+++  ++  L+NT+
Sbjct: 129  KKLNAIAEERKKFHL----QEKIIERQAATRETGSVLTEPQVYGRDKEKDEIVKILINTA 188

Query: 193  NITKDNVEVVSIVGMGGLGKTALAQTVYNDEKINNRFKWKIWVCISQEFDIKVIVEKILE 252
            +  +  + V+ I+GMGGLGKT L+Q V+ND+++  RF  KIW+CIS +F+ K +++ I+E
Sbjct: 189  SDAQ-KLSVLPILGMGGLGKTTLSQMVFNDQRVTERFYPKIWICISDDFNEKRLIKAIVE 248

Query: 253  SITKTKQESLQLDILQSMLQEKIYGKKYLLVMDDVWNVDHEKWIGLKRFLMGGASGSKIL 312
            SI       + L  LQ  LQE + GK+Y LV+DDVWN D  KW  L+  L  GASG+ +L
Sbjct: 249  SIEGKSLSDMDLAPLQKKLQELLNGKRYFLVLDDVWNEDQHKWANLRAVLKVGASGAFVL 308

Query: 313  VTTRNLQTAQASDTVWFHHLKELDKDNSWALFRKMAFLNKEEELENSNLVRIGKEIVAKL 372
             TTR  +      T+  + L  L  ++ W LF + AF ++EE   N NL+ IGKEIV K 
Sbjct: 309  TTTRLEKVGSIMGTLQPYELSNLSPEDCWFLFMQRAFGHQEE--INPNLMAIGKEIVKKC 368

Query: 373  KGYPLSIRVVGRLLYFKNTEMDWSSFKDNELDSILQEDDQIQPILKISFNHLPPKLKQCF 432
             G PL+ + +G +L FK  E +W   +D+ + ++ Q++  I P L++S++HLP  L+QCF
Sbjct: 369  GGVPLAAKTLGGILRFKREEREWEHVRDSPIWNLPQDESSILPALRLSYHHLPLDLRQCF 428

Query: 433  TYCALFPKDYEFKKNGLVKQWMAQGFIQAHNKKAIEDVGDDYFQELVGRSFFQDIRKNKW 492
             YCA+FPKD +  K  L+  WMA GF+ +     +EDVG++ + EL  RSFFQ+I     
Sbjct: 429  VYCAVFPKDTKMAKENLIAFWMAHGFLLSKGNLELEDVGNEVWNELYLRSFFQEIEVE-- 488

Query: 493  GDLKYCKMHDLLHDLACSIGENECVVVSDDVGSIDKRTRHASFLLSKRLTREVVSKSSIE 552
                Y KMHDL+HDLA S+                      S   S    RE+ +     
Sbjct: 489  SGKTYFKMHDLIHDLATSL---------------------FSANTSSSNIREINANYDGY 548

Query: 553  VTSLRTLDIDSRASFRSFKKTCHMNLFQLRTLNLDRCCCHPPKFVDKLKHLRYLNLSG-L 612
            + S+   ++ S  S    +K   + +  LR  NL++     P  +  L HLRYL+LSG  
Sbjct: 549  MMSIGFAEVVSSYSPSLLQKFVSLRVLNLRNSNLNQL----PSSIGDLVHLRYLDLSGNF 608

Query: 613  NVTFLPNSITTLYNLETLILRYCLWLRKLPKDINNLINLRHLDIYDCSSLTHMPKGLGGM 672
             +  LP  +  L NL+TL L YC  L  LPK  + L +LR+L + D  SLT  P  +G +
Sbjct: 609  RIRNLPKRLCKLQNLQTLDLHYCDSLSCLPKQTSKLGSLRNL-LLDGCSLTSTPPRIGLL 668

Query: 673  TSLQTMSMFVLGKNKGGDLSALNGLKSLRGLLCIKGLQFC---TTADLKNLELHWDI-KM 732
            T L+++S FV+GK KG  L  L  L +L G + I  L      T A   NL    ++  +
Sbjct: 669  TCLKSLSCFVIGKRKGHQLGELKNL-NLYGSISITKLDRVKKDTDAKEANLSAKANLHSL 728

Query: 733  DHEDALDDGDNDDEGVLEGLKPHSNIRKMIIKGYRGMKLCDWFSSNFLGGLVSIELSHCE 792
                 LD     D  VLE LKPHSN++ + I G+ G++L DW + + L  +VSI +  CE
Sbjct: 729  CLSWDLDGKHRYDSEVLEALKPHSNLKYLEINGFGGIRLPDWMNQSVLKNVVSIRIRGCE 788

Query: 793  KLEHLPQFDQFLYLKHLLLGY-LPNIEYIDSGNSVSSSTTFFPSLEKLRIESMPKLKGWW 852
                LP F +   L+ L L     ++EY++           FPSL KL I     LKG  
Sbjct: 789  NCSCLPPFGELPCLESLELHTGSADVEYVEDNVHPGR----FPSLRKLVIWDFSNLKGLL 848

Query: 853  K--GEISFPTTILHQLSELCIFYCPLLASIPQHPSLESLRICGVSVQLFQMVIRMATDLS 912
            K  GE  FP      L E+  ++CP+   IP   S+++L++          ++  AT L 
Sbjct: 849  KMEGEKQFPV-----LEEMTFYWCPMFV-IPTLSSVKTLKV----------IVTDATVL- 908

Query: 913  EHSSSSSTLSKLSFLEIG-TIDLEFLPVELFCNMTHLESLIIERCKSLQMSSPHPVDEDN 972
                S S L  L+ L+I   ++   LP E+F ++ +L+ L I   ++L         ++ 
Sbjct: 909  ---RSISNLRALTSLDISDNVEATSLPEEMFKSLANLKYLKISFFRNL---------KEL 952

Query: 973  DVVWKKLSNLRTLRLESILKLEYFP-KSLKYITSLETLKLSNCENL-VSTEGIGELISLS 1025
                  L+ L++L+ E    LE  P + +K +TSL  L +SNC  L    EG+  L +L+
Sbjct: 969  PTSLASLNALKSLKFEFCDALESLPEEGVKGLTSLTELSVSNCMMLKCLPEGLQHLTALT 952


HSP 2 Score: 51.2 bits (121), Expect = 8.5e-05
Identity = 35/112 (31.25%), Postives = 54/112 (48.21%), Query Frame = 1

Query: 961  VWKKLSNLRTLRLESILKLEYFPKSLKYITSLETLKLSNCENLVSTEGIGELISLSHLEI 1020
            ++K L+NL+ L++     L+  P SL  + +L++LK   C+ L S               
Sbjct: 863  MFKSLANLKYLKISFFRNLKELPTSLASLNALKSLKFEFCDALES--------------- 922

Query: 1021 DRCPNLPILSEDVGDLISLSHLLIWNCPKLTSLSEGITRLTSLSSLCLEDCP 1073
                 LP   E V  L SL+ L + NC  L  L EG+  LT+L++L +  CP
Sbjct: 923  -----LP--EEGVKGLTSLTELSVSNCMMLKCLPEGLQHLTALTTLTITQCP 952


HSP 3 Score: 48.1 bits (113), Expect = 7.2e-04
Identity = 55/181 (30.39%), Postives = 82/181 (45.30%), Query Frame = 1

Query: 953  PVDEDNDVVWKKLSNLRTLRLESILKLEYFPKS-LKYITSLETL-KLSNCENLVST---- 1012
            PV E+    W  +  + TL     LK+     + L+ I++L  L  L   +N+ +T    
Sbjct: 802  PVLEEMTFYWCPMFVIPTLSSVKTLKVIVTDATVLRSISNLRALTSLDISDNVEATSLPE 861

Query: 1013 EGIGELISLSHLEIDRCPNLPILSEDVGDLISLSHLLIWNCPKLTSL-SEGITRLTSLSS 1072
            E    L +L +L+I    NL  L   +  L +L  L    C  L SL  EG+  LTSL+ 
Sbjct: 862  EMFKSLANLKYLKISFFRNLKELPTSLASLNALKSLKFEFCDALESLPEEGVKGLTSLTE 921

Query: 1073 LCLEDCPNLVSLPQEFLHHHSSLPGGRFLRILNCPKLQIQDKKQKEEEEEDQEDWNELIH 1127
            L + +C  L  LP E L H ++L     L I  CP +       K  E    EDW+++ H
Sbjct: 922  LSVSNCMMLKCLP-EGLQHLTAL---TTLTITQCPIV------FKRCERGIGEDWHKIAH 972

BLAST of Cucsa.128100 vs. Swiss-Prot
Match: RGA2_SOLBU (Disease resistance protein RGA2 OS=Solanum bulbocastanum GN=RGA2 PE=1 SV=1)

HSP 1 Score: 493.8 bits (1270), Expect = 5.0e-138
Identity = 349/1023 (34.12%), Postives = 534/1023 (52.20%), Query Frame = 1

Query: 13   VITKLGSSALRELGSLWGVNDELDKLQNTLSAIKAVLLDAEEQQSKSHTVKDWIAKIKDV 72
            ++  L S    EL  L+G  DE  +L +  S I+AVL DA+E+Q  +  +++W+ K+   
Sbjct: 9    LLDNLTSFLKGELVLLFGFQDEFQRLSSMFSTIQAVLEDAQEKQLNNKPLENWLQKLNAA 68

Query: 73   FYDIDDLIDEFSYETLRRQVLTKDRTITKQVRIFFSKSNQIAFGFKMGQTIKKVREKLDA 132
             Y++DD++DE+  +  R       R   K           I F  K+G+ + +V +KL A
Sbjct: 69   TYEVDDILDEYKTKATRFSQSEYGRYHPKV----------IPFRHKVGKRMDQVMKKLKA 128

Query: 133  IAAIKAQLHLSVCAREVRDNEPRKVRETSSFIPEGEIIGRDEDRKSVMDFLLNTSNITKD 192
            IA  +   HL     ++ + +  + RET S + E ++ GRD+++  ++  L+N  +  + 
Sbjct: 129  IAEERKNFHLH---EKIVERQAVR-RETGSVLTEPQVYGRDKEKDEIVKILINNVSDAQ- 188

Query: 193  NVEVVSIVGMGGLGKTALAQTVYNDEKINNRFKWKIWVCISQEFDIKVIVEKILESIT-K 252
            ++ V+ I+GMGGLGKT LAQ V+ND+++   F  KIW+C+S++FD K +++ I+ESI  +
Sbjct: 189  HLSVLPILGMGGLGKTTLAQMVFNDQRVTEHFHSKIWICVSEDFDEKRLIKAIVESIEGR 248

Query: 253  TKQESLQLDILQSMLQEKIYGKKYLLVMDDVWNVDHEKWIGLKRFLMGGASGSKILVTTR 312
                 + L  LQ  LQE + GK+YLLV+DDVWN D +KW  L+  L  GASG+ +L TTR
Sbjct: 249  PLLGEMDLAPLQKKLQELLNGKRYLLVLDDVWNEDQQKWANLRAVLKVGASGASVLTTTR 308

Query: 313  NLQTAQASDTVWFHHLKELDKDNSWALFRKMAFLNKEEELENSNLVRIGKEIVAKLKGYP 372
              +      T+  + L  L +++ W LF + AF ++EE   N NLV IGKEIV K  G P
Sbjct: 309  LEKVGSIMGTLQPYELSNLSQEDCWLLFMQRAFGHQEEI--NPNLVAIGKEIVKKSGGVP 368

Query: 373  LSIRVVGRLLYFKNTEMDWSSFKDNELDSILQEDDQIQPILKISFNHLPPKLKQCFTYCA 432
            L+ + +G +L FK  E  W   +D+ + ++ Q++  I P L++S++ LP  LKQCF YCA
Sbjct: 369  LAAKTLGGILCFKREERAWEHVRDSPIWNLPQDESSILPALRLSYHQLPLDLKQCFAYCA 428

Query: 433  LFPKDYEFKKNGLVKQWMAQGFIQAHNKKAIEDVGDDYFQELVGRSFFQDIRKNKWGDLK 492
            +FPKD + +K  L+  WMA GF+ +     +EDVGD+ ++EL  RSFFQ+I         
Sbjct: 429  VFPKDAKMEKEKLISLWMAHGFLLSKGNMELEDVGDEVWKELYLRSFFQEIEVKD--GKT 488

Query: 493  YCKMHDLLHDLACSIGENECVVVSDDVGSIDKRTRHASFLLSKRLTREVVSKSSIEVTSL 552
            Y KMHDL+HDLA S+        S +  S + R       ++K     ++S    EV   
Sbjct: 489  YFKMHDLIHDLATSL-------FSANTSSSNIRE------INKHSYTHMMSIGFAEVVFF 548

Query: 553  RTLDIDSRASFRSFKKTCHMNLFQLRTLNL-DRCCCHPPKFVDKLKHLRYLNLSGLNVTF 612
             TL    +                LR LNL D      P  +  L HLRYLNL G  +  
Sbjct: 549  YTLPPLEK-------------FISLRVLNLGDSTFNKLPSSIGDLVHLRYLNLYGSGMRS 608

Query: 613  LPNSITTLYNLETLILRYCLWLRKLPKDINNLINLRHLDIYDCSSLTHMPKGLGGMTSLQ 672
            LP  +  L NL+TL L+YC  L  LPK+ + L +LR+L +    SLT MP  +G +T L+
Sbjct: 609  LPKQLCKLQNLQTLDLQYCTKLCCLPKETSKLGSLRNLLLDGSQSLTCMPPRIGSLTCLK 668

Query: 673  TMSMFVLGKNKGGDLSALNGLKSLRGLLCIKGLQ------------FCTTADLKNLELHW 732
            T+  FV+G+ KG  L  L  L +L G + I  L+                 +L +L + W
Sbjct: 669  TLGQFVVGRKKGYQLGELGNL-NLYGSIKISHLERVKNDKDAKEANLSAKGNLHSLSMSW 728

Query: 733  DIKMDHEDALDDGDNDDEGVLEGLKPHSNIRKMIIKGYRGMKLCDWFSSNFLGGLVSIEL 792
            +    H       ++++  VLE LKPHSN+  + I G+RG+ L +W + + L  +VSI +
Sbjct: 729  NNFGPHIY-----ESEEVKVLEALKPHSNLTSLKIYGFRGIHLPEWMNHSVLKNIVSILI 788

Query: 793  SHCEKLEHLPQFDQFLYLKHLLLGY-LPNIEYIDSGN----SVSSSTTFFPSLEKLRIES 852
            S+      LP F     L+ L L +   ++EY++  +    S   +   FPSL KL I  
Sbjct: 789  SNFRNCSCLPPFGDLPCLESLELHWGSADVEYVEEVDIDVHSGFPTRIRFPSLRKLDIWD 848

Query: 853  MPKLKGWWK--GEISFPTTILHQLSELCIFYCPLLASIPQHPSLESLRICGVSVQLFQMV 912
               LKG  K  GE  FP      L E+ I  CP L       +L SLRIC          
Sbjct: 849  FGSLKGLLKKEGEEQFPV-----LEEMIIHECPFLTLSSNLRALTSLRIC---------Y 908

Query: 913  IRMATDLSEHSSSSSTLSKLSFLEIGTID-LEFLPVELFCNMTHLESLIIERCKSLQMSS 972
             ++AT   E    +  L+ L +L I   + L+ LP  L  ++  L+SL I+ C +L+   
Sbjct: 909  NKVATSFPEEMFKN--LANLKYLTISRCNNLKELPTSL-ASLNALKSLKIQLCCALESLP 955

Query: 973  PHPVDEDNDVVWKKLSNLRTLRLESILKLEYFPKSLKYITSLETLKLSNCENLVS--TEG 1012
               ++         LS+L  L +E    L+  P+ L+++T+L +LK+  C  L+    +G
Sbjct: 969  EEGLEG--------LSSLTELFVEHCNMLKCLPEGLQHLTTLTSLKIRGCPQLIKRCEKG 955


HSP 2 Score: 60.5 bits (145), Expect = 1.4e-07
Identity = 109/454 (24.01%), Postives = 190/454 (41.85%), Query Frame = 1

Query: 639  INNLINLRHLDIYDCSSLTHMPKGLGGMTSLQTMSMFVLG-KNKGGDLSALNGLKSLRGL 698
            +   I+LR L++ D S+   +P  +G +  L+ ++++  G ++    L  L  L++L   
Sbjct: 522  LEKFISLRVLNLGD-STFNKLPSSIGDLVHLRYLNLYGSGMRSLPKQLCKLQNLQTLDLQ 581

Query: 699  LCIKGLQFCTTADLKNLELHWDIKMDHEDALDDGDNDDEGVLEGLKPHSNIRKMIIKGYR 758
             C K    C   +   L    ++ +D   +L        G L  LK    + + ++   +
Sbjct: 582  YCTK--LCCLPKETSKLGSLRNLLLDGSQSLTCMP-PRIGSLTCLK---TLGQFVVGRKK 641

Query: 759  GMKLCDWFSSNFLGGLVSIELSHCEKLEHLPQFDQFLYLKHLLLGYLPNIEYIDSGNSVS 818
            G +L +  + N  G   SI++SH E++++     +        L  L ++ + + G  + 
Sbjct: 642  GYQLGELGNLNLYG---SIKISHLERVKNDKDAKEANLSAKGNLHSL-SMSWNNFGPHIY 701

Query: 819  SSTTFFPSLEKLRIES-MPKLKGWWKGEISFPTTILHQLSELCIFY-------CPLLASI 878
             S      LE L+  S +  LK +    I  P  + H + +  +         C  L   
Sbjct: 702  ESEEV-KVLEALKPHSNLTSLKIYGFRGIHLPEWMNHSVLKNIVSILISNFRNCSCLPPF 761

Query: 879  PQHPSLESLRICGVSVQLFQMVIRMATDLSEHSSSSS-----TLSKLSFLEIGTIDLEFL 938
               P LESL +   S  +  +      D+  HS   +     +L KL   + G++    L
Sbjct: 762  GDLPCLESLELHWGSADVEYVE---EVDIDVHSGFPTRIRFPSLRKLDIWDFGSLK-GLL 821

Query: 939  PVELFCNMTHLESLIIERCKSLQMSSPHPVDEDNDVVWKKLSNLRTLRLESILKLEYFPK 998
              E       LE +II  C  L +SS              L  L +LR+        FP+
Sbjct: 822  KKEGEEQFPVLEEMIIHECPFLTLSS-------------NLRALTSLRICYNKVATSFPE 881

Query: 999  SL-KYITSLETLKLSNCENLVSTE-GIGELISLSHLEIDRCPNLPILSED-VGDLISLSH 1058
             + K + +L+ L +S C NL      +  L +L  L+I  C  L  L E+ +  L SL+ 
Sbjct: 882  EMFKNLANLKYLTISRCNNLKELPTSLASLNALKSLKIQLCCALESLPEEGLEGLSSLTE 941

Query: 1059 LLIWNCPKLTSLSEGITRLTSLSSLCLEDCPNLV 1076
            L + +C  L  L EG+  LT+L+SL +  CP L+
Sbjct: 942  LFVEHCNMLKCLPEGLQHLTTLTSLKIRGCPQLI 946


HSP 3 Score: 40.0 bits (92), Expect = 2.0e-01
Identity = 43/156 (27.56%), Postives = 63/156 (40.38%), Query Frame = 1

Query: 992  LETLKLSNCENLVSTEGIGELISLSHLEIDRCPNLPILSEDVGDLISLSHLLIWNCPKLT 1051
            LE + +  C  L  +  +  L SL         + P   E   +L +L +L I  C  L 
Sbjct: 816  LEEMIIHECPFLTLSSNLRALTSLRICYNKVATSFP--EEMFKNLANLKYLTISRCNNLK 875

Query: 1052 SLSEGITRLTSLSSLCLEDCPNLVSLPQEFLHHHSS--------------LPGG------ 1111
             L   +  L +L SL ++ C  L SLP+E L   SS              LP G      
Sbjct: 876  ELPTSLASLNALKSLKIQLCCALESLPEEGLEGLSSLTELFVEHCNMLKCLPEGLQHLTT 935

Query: 1112 -RFLRILNCPKLQIQDKKQKEEEEEDQEDWNELIHV 1127
               L+I  CP+L       K  E+   EDW+++ H+
Sbjct: 936  LTSLKIRGCPQL------IKRCEKGIGEDWHKISHI 963

BLAST of Cucsa.128100 vs. Swiss-Prot
Match: RGA3_SOLBU (Putative disease resistance protein RGA3 OS=Solanum bulbocastanum GN=RGA3 PE=2 SV=2)

HSP 1 Score: 493.8 bits (1270), Expect = 5.0e-138
Identity = 353/1072 (32.93%), Postives = 532/1072 (49.63%), Query Frame = 1

Query: 24   ELGSLWGVNDELDKLQNTLSAIKAVLLDAEEQQSKSHTVKDWIAKIKDVFYDIDDLIDEF 83
            ELG ++G   E  KL +  S I+AVL DA+E+Q K   +K+W+ K+    Y++DD++D+ 
Sbjct: 20   ELGLVFGFEKEFKKLSSMFSMIQAVLEDAQEKQLKYKAIKNWLQKLNVAAYEVDDILDDC 79

Query: 84   SYETLRRQVLTKDRTITKQVRIFFSKSNQIAFGFKMGQTIKKVREKLDAIAAIKAQLHLS 143
              E  R           KQ  +       I F +K+G+ +K++ EKLDAIA  +   HL 
Sbjct: 80   KTEAAR----------FKQAVLGRYHPRTITFCYKVGKRMKEMMEKLDAIAEERRNFHLD 139

Query: 144  VCAREVRDNEPRKVRETSSFIPEGEIIGRDEDRKSVMDFLLNTSNITKDNVEVVSIVGMG 203
               R +     R  R+T   + E ++ GR+++   ++  L+N  + +++ V V+ I+GMG
Sbjct: 140  --ERIIERQAAR--RQTGFVLTEPKVYGREKEEDEIVKILINNVSYSEE-VPVLPILGMG 199

Query: 204  GLGKTALAQTVYNDEKINNRFKWKIWVCISQEFDIKVIVEKILESITKTKQESLQLDILQ 263
            GLGKT LAQ V+ND++I   F  KIWVC+S +FD K +++ I+ESI       + L  LQ
Sbjct: 200  GLGKTTLAQMVFNDQRITEHFNLKIWVCVSDDFDEKRLIKAIVESIEGKSLGDMDLAPLQ 259

Query: 264  SMLQEKIYGKKYLLVMDDVWNVDHEKWIGLKRFLMGGASGSKILVTTRNLQTAQASDTVW 323
              LQE + GK+Y LV+DDVWN D EKW  L+  L  GASG+ IL+TTR  +      T+ 
Sbjct: 260  KKLQELLNGKRYFLVLDDVWNEDQEKWDNLRAVLKIGASGASILITTRLEKIGSIMGTLQ 319

Query: 324  FHHLKELDKDNSWALFRKMAFLNKEEELENSNLVRIGKEIVAKLKGYPLSIRVVGRLLYF 383
             + L  L +++ W LF++ AF ++ E   +  L+ IGKEIV K  G PL+ + +G LL F
Sbjct: 320  LYQLSNLSQEDCWLLFKQRAFCHQTET--SPKLMEIGKEIVKKCGGVPLAAKTLGGLLRF 379

Query: 384  KNTEMDWSSFKDNELDSILQEDDQIQPILKISFNHLPPKLKQCFTYCALFPKDYEFKKNG 443
            K  E +W   +D+E+ ++ Q+++ + P L++S++HLP  L+QCF YCA+FPKD + +K  
Sbjct: 380  KREESEWEHVRDSEIWNLPQDENSVLPALRLSYHHLPLDLRQCFAYCAVFPKDTKIEKEY 439

Query: 444  LVKQWMAQGFIQAHNKKAIEDVGDDYFQELVGRSFFQDIRKNKWGDLKYCKMHDLLHDLA 503
            L+  WMA  F+ +     +EDVG++ + EL  RSFFQ+I         Y KMHDL+HDLA
Sbjct: 440  LIALWMAHSFLLSKGNMELEDVGNEVWNELYLRSFFQEIEVKS--GKTYFKMHDLIHDLA 499

Query: 504  CSIGENECVVVSDDVGSIDKRTRHASFLLSKRLTREVVSKSSIEVTSLRT-----LDIDS 563
             S+                      S   S R  R++  K   ++  + T     + I  
Sbjct: 500  TSM---------------------FSASASSRSIRQINVKDDEDMMFIVTNYKDMMSIGF 559

Query: 564  RASFRSFKKTCHMNLFQLRTLNLDRCCCHP-PKFVDKLKHLRYLNLSGLNVTFLPNSITT 623
                 S+  +       LR LNL        P  V  L HLRYL+LSG  +  LP  +  
Sbjct: 560  SEVVSSYSPSLFKRFVSLRVLNLSNSEFEQLPSSVGDLVHLRYLDLSGNKICSLPKRLCK 619

Query: 624  LYNLETLILRYCLWLRKLPKDINNLINLRHLDIYDCSSLTHMPKGLGGMTSLQTMSMFVL 683
            L NL+TL L  C  L  LPK  + L +LR+L +  C  LT MP  +G +T L+T+  FV+
Sbjct: 620  LQNLQTLDLYNCQSLSCLPKQTSKLCSLRNLVLDHCP-LTSMPPRIGLLTCLKTLGYFVV 679

Query: 684  GKNKGGDLSALNGLKSLRGLLCIKGLQ------------FCTTADLKNLELHWDIKMDHE 743
            G+ KG  L  L  L +LRG + I  L+                A+L +L + WD    +E
Sbjct: 680  GERKGYQLGELRNL-NLRGAISITHLERVKNDMEAKEANLSAKANLHSLSMSWDRPNRYE 739

Query: 744  DALDDGDNDDEGVLEGLKPHSNIRKMIIKGYRGMKLCDWFSSNFLGGLVSIELSHCEKLE 803
                   +++  VLE LKPH N++ + I  + G  L DW + + L  +VSI +S CE   
Sbjct: 740  -------SEEVKVLEALKPHPNLKYLEIIDFCGFCLPDWMNHSVLKNVVSILISGCENCS 799

Query: 804  HLPQFDQFLYLKHL-LLGYLPNIEYIDSGNSVSSSTTFFPSLEKLRIESMPKLKGWWKGE 863
             LP F +   L+ L L      +EY++    ++     FPSL KL I     LKG  +  
Sbjct: 800  CLPPFGELPCLESLELQDGSVEVEYVEDSGFLTRRR--FPSLRKLHIGGFCNLKGLQR-- 859

Query: 864  ISFPTTILHQLSELCIFYCPLLASIPQHPSLESLRICGVSVQLFQMVIRMATDLSEHSSS 923
                                 +    Q P LE ++I    + +F                
Sbjct: 860  ---------------------MKGAEQFPVLEEMKISDCPMFVFP--------------- 919

Query: 924  SSTLSKLSFLEI-GTIDLEFLPVELFCNMTHLESLIIERCKSLQMSSPHPVDEDNDVVWK 983
              TLS +  LEI G  D   L      ++++L +L      SL++ S H V    + ++K
Sbjct: 920  --TLSSVKKLEIWGEADAGGLS-----SISNLSTLT-----SLKIFSNHTVTSLLEEMFK 968

Query: 984  KLSNLRTLRLESILKLEYFPKSLKYITSLETLKLSNCENLVSTEGIGELISLSHLEIDRC 1043
             L NL  L +  +  L+  P SL  + +L+ L +  C  L S                  
Sbjct: 980  NLENLIYLSVSFLENLKELPTSLASLNNLKCLDIRYCYALES------------------ 968

Query: 1044 PNLPILSEDVGDLISLSHLLIWNCPKLTSLSEGITRLTSLSSLCLEDCPNLV 1076
              LP   E +  L SL+ L + +C  L  L EG+  LT+L+SL +  CP L+
Sbjct: 1040 --LP--EEGLEGLSSLTELFVEHCNMLKCLPEGLQHLTTLTSLKIRGCPQLI 968

BLAST of Cucsa.128100 vs. Swiss-Prot
Match: R13L1_ARATH (Putative disease resistance RPP13-like protein 1 OS=Arabidopsis thaliana GN=RPPL1 PE=3 SV=1)

HSP 1 Score: 431.0 bits (1107), Expect = 4.0e-119
Identity = 315/1011 (31.16%), Postives = 515/1011 (50.94%), Query Frame = 1

Query: 35   LDKLQNTLSAIKAVLLDAEEQQSKSHTVKDWIAKIKDVFYDIDDLIDEFSYETLRRQVLT 94
            L++L   L  I AVL+DAEE+Q  +  V+ W+ +++DV Y  +D +D+ + E LR  +  
Sbjct: 39   LERLSTALLTITAVLIDAEEKQITNPVVEKWVNELRDVVYHAEDALDDIATEALRLNIGA 98

Query: 95   KDRTITK--QVRIFFSKSNQIAFGFKMGQT-IKKVREKLDAIAAIKAQLHLSVCAREVRD 154
            +  +  +  Q+R   S  + +    +  +T ++KV  +L+ +A+ +  L L    +E+  
Sbjct: 99   ESSSSNRLRQLRGRMSLGDFLDGNSEHLETRLEKVTIRLERLASQRNILGL----KELTA 158

Query: 155  NEPRKVRETSSFIPEGEIIGRDEDRKSVMDFLLNTSNITKDN-VEVVSIVGMGGLGKTAL 214
              P++   T+S + E E+ GRD+D+  +M FL+  +   KDN + VV+IVG+GG+GKT L
Sbjct: 159  MIPKQRLPTTSLVDESEVFGRDDDKDEIMRFLIPENG--KDNGITVVAIVGIGGVGKTTL 218

Query: 215  AQTVYNDEKINNRFKWKIWVCISQEFDIKVIVEKILESITKTKQESLQLDILQSMLQEKI 274
            +Q +YND+ + + F  K+W  +S+EFD+  I +K+ ES+T    E   LD+LQ  L+E++
Sbjct: 219  SQLLYNDQHVRSYFGTKVWAHVSEEFDVFKITKKVYESVTSRPCEFTDLDVLQVKLKERL 278

Query: 275  YGK--KYLLVMDDVWNVDHEKWIGLKRFLMGGASGSKILVTTRNLQTAQASDTVWFHHLK 334
             G    +LLV+DD+WN +   W  L++  +  A GS+ILVTTR+ + A     V  H+L+
Sbjct: 279  TGTGLPFLLVLDDLWNENFADWDLLRQPFIHAAQGSQILVTTRSQRVASIMCAVHVHNLQ 338

Query: 335  ELDKDNSWALFRKMAFLNKEEELENSNLVRIGKEIVAKLKGYPLSIRVVGRLLYFKNTEM 394
             L   + W+LF K  F N+E  L N  +  + + IV K +G PL+++ +G +L F+   +
Sbjct: 339  PLSDGDCWSLFMKTVFGNQEPCL-NREIGDLAERIVHKCRGLPLAVKTLGGVLRFEGKVI 398

Query: 395  DWSSFKDNELDSILQEDDQIQPILKISFNHLPPKLKQCFTYCALFPKDYEFKKNGLVKQW 454
            +W     + +  +  +   + P+L++S+ +LP  LK+CF YC++FPK + F+K+ +V  W
Sbjct: 399  EWERVLSSRIWDLPADKSNLLPVLRVSYYYLPAHLKRCFAYCSIFPKGHAFEKDKVVLLW 458

Query: 455  MAQGFI-QAHNKKAIEDVGDDYFQELVGRSFFQDIRKNKWGDLKYCKMHDLLHDLA-CSI 514
            MA+GF+ Q  + K +E++G++YF EL  RS  Q  +       +Y  MHD +++LA  + 
Sbjct: 459  MAEGFLQQTRSSKNLEELGNEYFSELESRSLLQKTK------TRYI-MHDFINELAQFAS 518

Query: 515  GENECVVVSDDVGSIDKRTRHASFLLSKRLTREVVSKSSIEVTSLRT-LDIDSRASFRS- 574
            GE            + +RTR+ S+ L       +  ++  EV  LRT L +    S RS 
Sbjct: 519  GEFSSKFEDGCKLQVSERTRYLSY-LRDNYAEPMEFEALREVKFLRTFLPLSLTNSSRSC 578

Query: 575  -----FKKTCHMNLFQLRTLNLD--RCCCHPPKFVDKLKHLRYLNLSGLNVTFLPNSITT 634
                   +     L +LR L+L   +    PP F   + H R+L+LS   +  LP S+  
Sbjct: 579  CLDQMVSEKLLPTLTRLRVLSLSHYKIARLPPDFFKNISHARFLDLSRTELEKLPKSLCY 638

Query: 635  LYNLETLILRYCLWLRKLPKDINNLINLRHLDIYDCSSLTHMPKGLGGMTSLQTMSMFVL 694
            +YNL+TL+L YC  L++LP DI+NLINLR+LD+   + L  MP+  G + SLQT++ F +
Sbjct: 639  MYNLQTLLLSYCSSLKELPTDISNLINLRYLDLIG-TKLRQMPRRFGRLKSLQTLTTFFV 698

Query: 695  GKNKGGDLSALNGLKSLRGLLCIKGLQ-FCTTAD-----------LKNLELHW--DIKMD 754
              + G  +S L GL  L G L I  LQ     AD           L+ ++  W       
Sbjct: 699  SASDGSRISELGGLHDLHGKLKIVELQRVVDVADAAEANLNSKKHLREIDFVWRTGSSSS 758

Query: 755  HEDALDDGDNDDEGVLEGLKPHSNIRKMIIKGYRGMKLCDWFSSNFLGGLVSIELSHCEK 814
              +       ++  V E L+PH +I K+ I+ Y+G +  DW S      +V I L  C+ 
Sbjct: 759  ENNTNPHRTQNEAEVFEKLRPHRHIEKLAIERYKGRRFPDWLSDPSFSRIVCIRLRECQY 818

Query: 815  LEHLPQFDQFLYLKHLLLGYLPNIE------YIDSGNSVSSSTTFFPSLEKLRIESMPKL 874
               LP   Q   LK L +  +  ++      Y             F SLE LR +++P  
Sbjct: 819  CTSLPSLGQLPCLKELHISGMVGLQSIGRKFYFSDQQLRDQDQQPFRSLETLRFDNLPDW 878

Query: 875  KGWWKGEISFPTTILHQLSELCIFYCP-LLASIPQH-PSLESLRICGVSVQLFQMVIRMA 934
            + W    ++    +   L +L I  CP L  ++P   PSL SL I    +  FQ      
Sbjct: 879  QEWLDVRVT-RGDLFPSLKKLFILRCPELTGTLPTFLPSLISLHIYKCGLLDFQP----- 938

Query: 935  TDLSEHSSSSSTLSKLSFLEIGTIDLEFLPVELFCNMTHLESLIIERCK---SLQMSSPH 994
                 H  S   L  LS ++     L   P+  F N+  LE   +++C    SL++S+ H
Sbjct: 939  ---DHHEYSYRNLQTLS-IKSSCDTLVKFPLNHFANLDKLE---VDQCTSLYSLELSNEH 998

Query: 995  PVDEDNDVVWKKLSNLRTLRLESILKLEYFPKSLKYITSLETLKLSNCENL 1004
                      +  + LR LR+     L+  PK L  +     + ++NC  L
Sbjct: 999  ---------LRGPNALRNLRINDCQNLQLLPK-LNALPQNLQVTITNCRYL 1010

BLAST of Cucsa.128100 vs. TrEMBL
Match: A0A0A0K9P7_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_7G425940 PE=3 SV=1)

HSP 1 Score: 1580.1 bits (4090), Expect = 0.0e+00
Identity = 781/793 (98.49%), Postives = 781/793 (98.49%), Query Frame = 1

Query: 350  ELENSNLVRIGKEIVAKLKGYPLSIRVVGRLLYFKNTEMDWSSFKDNELDSILQEDDQIQ 409
            ELENSNLVRIGKEIVAKLKGYPLSIRVVGRLLYFKNTEMDWSSFKDNELDSILQEDDQIQ
Sbjct: 171  ELENSNLVRIGKEIVAKLKGYPLSIRVVGRLLYFKNTEMDWSSFKDNELDSILQEDDQIQ 230

Query: 410  PILKISFNHLPPKLKQCFTYCALFPKDYEFKKNGLVKQWMAQGFIQAHNKKAIEDVGDDY 469
            PILKISFNHLPPKLKQCFTYCALFPKDYEFKKNGLVKQWMAQGFIQAHNKKAIEDVGDDY
Sbjct: 231  PILKISFNHLPPKLKQCFTYCALFPKDYEFKKNGLVKQWMAQGFIQAHNKKAIEDVGDDY 290

Query: 470  FQELVGRSFFQDIRKNKWGDLKYCKMHDLLHDLACSIGENECVVVSDDVGSIDKRTRHAS 529
            FQELVGRSFFQDIRKNKWGDLKYCKMHDLLHDLACSIGENECVVVSDDVGSIDKRTRHAS
Sbjct: 291  FQELVGRSFFQDIRKNKWGDLKYCKMHDLLHDLACSIGENECVVVSDDVGSIDKRTRHAS 350

Query: 530  FLLSKRLTREVVSKSSIEVTSLRTLDIDSRASFRSFKKTCHMNLFQLRTLNLDRCCCHPP 589
            FLLSKRLTREVVSKSSIEVTSLRTLDIDSRASFRSFKKTCHMNLFQLRTLNLDRCCCHPP
Sbjct: 351  FLLSKRLTREVVSKSSIEVTSLRTLDIDSRASFRSFKKTCHMNLFQLRTLNLDRCCCHPP 410

Query: 590  KFVDKLKHLRYLNLSGLNVTFLPNSITTLYNLETLILRYCLWLRKLPKDINNLINLRHLD 649
            KFVDKLKHLRYLNLSGLNVTFLPNSITTLYNLETLILRYCLWLRKLPKDINNLINLRHLD
Sbjct: 411  KFVDKLKHLRYLNLSGLNVTFLPNSITTLYNLETLILRYCLWLRKLPKDINNLINLRHLD 470

Query: 650  IYDCSSLTHMPKGLGGMTSLQTMSMFVLGKNKGGDLSALNGLKSLRGLLCIKGLQFCTTA 709
            IYDCSSLTHMPKGLGGMTSLQTMSMFVLGKNKGGDLSALNGLKSLRGLLCIKGLQFCTTA
Sbjct: 471  IYDCSSLTHMPKGLGGMTSLQTMSMFVLGKNKGGDLSALNGLKSLRGLLCIKGLQFCTTA 530

Query: 710  DLKN------------LELHWDIKMDHEDALDDGDNDDEGVLEGLKPHSNIRKMIIKGYR 769
            DLKN            LELHWDIKMDHEDALDDGDNDDEGVLEGLKPHSNIRKMIIKGYR
Sbjct: 531  DLKNVSYLKEMYGIQKLELHWDIKMDHEDALDDGDNDDEGVLEGLKPHSNIRKMIIKGYR 590

Query: 770  GMKLCDWFSSNFLGGLVSIELSHCEKLEHLPQFDQFLYLKHLLLGYLPNIEYIDSGNSVS 829
            GMKLCDWFSSNFLGGLVSIELSHCEKLEHLPQFDQFLYLKHLLLGYLPNIEYIDSGNSVS
Sbjct: 591  GMKLCDWFSSNFLGGLVSIELSHCEKLEHLPQFDQFLYLKHLLLGYLPNIEYIDSGNSVS 650

Query: 830  SSTTFFPSLEKLRIESMPKLKGWWKGEISFPTTILHQLSELCIFYCPLLASIPQHPSLES 889
            SSTTFFPSLEKLRIESMPKLKGWWKGEISFPTTILHQLSELCIFYCPLLASIPQHPSLES
Sbjct: 651  SSTTFFPSLEKLRIESMPKLKGWWKGEISFPTTILHQLSELCIFYCPLLASIPQHPSLES 710

Query: 890  LRICGVSVQLFQMVIRMATDLSEHSSSSSTLSKLSFLEIGTIDLEFLPVELFCNMTHLES 949
            LRICGVSVQLFQMVIRMATDLSEHSSSSSTLSKLSFLEIGTIDLEFLPVELFCNMTHLES
Sbjct: 711  LRICGVSVQLFQMVIRMATDLSEHSSSSSTLSKLSFLEIGTIDLEFLPVELFCNMTHLES 770

Query: 950  LIIERCKSLQMSSPHPVDEDNDVVWKKLSNLRTLRLESILKLEYFPKSLKYITSLETLKL 1009
            LIIERCKSLQMSSPHPVDEDNDVVWKKLSNLRTLRLESILKLEYFPKSLKYITSLETLKL
Sbjct: 771  LIIERCKSLQMSSPHPVDEDNDVVWKKLSNLRTLRLESILKLEYFPKSLKYITSLETLKL 830

Query: 1010 SNCENLVSTEGIGELISLSHLEIDRCPNLPILSEDVGDLISLSHLLIWNCPKLTSLSEGI 1069
            SNCENLVSTEGIGELISLSHLEIDRCPNLPILSEDVGDLISLSHLLIWNCPKLTSLSEGI
Sbjct: 831  SNCENLVSTEGIGELISLSHLEIDRCPNLPILSEDVGDLISLSHLLIWNCPKLTSLSEGI 890

Query: 1070 TRLTSLSSLCLEDCPNLVSLPQEFLHHHSSLPGGRFLRILNCPKLQIQDKKQKEEEEEDQ 1129
            TRLTSLSSLCLEDCPNLVSLPQEFLHHHSSLPGGRFLRILNCPKLQIQDKKQKEEEEEDQ
Sbjct: 891  TRLTSLSSLCLEDCPNLVSLPQEFLHHHSSLPGGRFLRILNCPKLQIQDKKQKEEEEEDQ 950

Query: 1130 EDWNELIHVLTGC 1131
            EDWNELIHVLTGC
Sbjct: 951  EDWNELIHVLTGC 963

BLAST of Cucsa.128100 vs. TrEMBL
Match: A0A0A0K9P7_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_7G425940 PE=3 SV=1)

HSP 1 Score: 328.6 bits (841), Expect = 3.1e-86
Identity = 175/198 (88.38%), Postives = 179/198 (90.40%), Query Frame = 1

Query: 1   MADSVLFNVAASVITKLGSSALRELGSLWGVNDELDKLQNTLSAIKAVLLDAEEQQSKSH 60
           MADSVLFNVAASVITKLGSSALRELGSLWGVNDELDKLQNTLSAIKAVLLDAEEQQSKSH
Sbjct: 1   MADSVLFNVAASVITKLGSSALRELGSLWGVNDELDKLQNTLSAIKAVLLDAEEQQSKSH 60

Query: 61  TVKDWIAKIKDVFYDIDDLIDEFSYETLRRQVLTKDRTITKQVRIFFSKSNQIAFGFKMG 120
           TVKDWIAKIKDVFYDIDDLIDEFSYETLRRQVLTKDRTITKQVRIFFSKSNQIAFGFKMG
Sbjct: 61  TVKDWIAKIKDVFYDIDDLIDEFSYETLRRQVLTKDRTITKQVRIFFSKSNQIAFGFKMG 120

Query: 121 QTIKKVREKLDAIAAIKAQLHLSVCAREVRDNEPRKVRETSSFIPEGEIIGRDEDRKSVM 180
           QTIKKVREKLDAIAAIKAQLHLSVCAREVRDNEPRKVRETSSFIPEGEII          
Sbjct: 121 QTIKKVREKLDAIAAIKAQLHLSVCAREVRDNEPRKVRETSSFIPEGEIIE--------- 180

Query: 181 DFLLNTSNITKDNVEVVS 199
              L  SN+ +   E+V+
Sbjct: 181 ---LENSNLVRIGKEIVA 186


HSP 2 Score: 791.2 bits (2042), Expect = 1.7e-225
Identity = 440/739 (59.54%), Postives = 521/739 (70.50%), Query Frame = 1

Query: 388  MDWSSFKDNELDSILQEDDQIQPILKISFNHLPPKLKQCFTYCALFPKDYEFKKNGLVKQ 447
            MDW SFKDN+L +ILQ+++QIQPILKISFNHLP  LK CFTYCALFPKDYEF+K+GLVKQ
Sbjct: 1    MDWLSFKDNDLGTILQQENQIQPILKISFNHLPSNLKHCFTYCALFPKDYEFQKDGLVKQ 60

Query: 448  WMAQGFIQAHNKKAIEDVGDDYFQELVGRSFFQDIRKNKWGDLKYCKMHDLLHDLACSIG 507
            WMAQGFIQ+H+ K IEDVGDDYF+EL+GRSFF +++ NKWGD+K CKMHDL+HDLAC I 
Sbjct: 61   WMAQGFIQSHSNKEIEDVGDDYFKELLGRSFFHNVKVNKWGDVKECKMHDLIHDLACWIV 120

Query: 508  ENECVVVSDDVGSIDKRTRHASFLLS-KRLTREVVSKSSIEVTSLRTLDIDSRASFRSFK 567
            ENECV  SD   SIDKRTRH SF  +  R + E+ +KS  EV +LRTL            
Sbjct: 121  ENECVDASDKTKSIDKRTRHVSFPSNYSRKSWELEAKSLTEVKNLRTL--------HGPP 180

Query: 568  KTCHMNLFQLRTLNLDRCCCHP-PKFVDKLKHLRYLNLSGLNVTFLPNSITTLYNLETLI 627
                 N  +LR+LNL        PKF+ +L+HLRYL++S  ++ FLP  IT LYNLETLI
Sbjct: 181  FLLSENHLRLRSLNLGYSKFQKIPKFISQLRHLRYLDISDHDMKFLPKFITKLYNLETLI 240

Query: 628  LRYCLWLRKLPKDINNLINLRHLDIYDCSSLTHMPKGLGGMTSLQTMSMFVLGKNKGGDL 687
            LR+C  LR+LP DINNLINL+HLD++ C  LTHMPKGLGG+TSLQTM++FVLGK+KG DL
Sbjct: 241  LRHCSDLRELPTDINNLINLKHLDVHGCYRLTHMPKGLGGLTSLQTMNLFVLGKDKGCDL 300

Query: 688  SALNGLKSLRGLLCIKGLQFCTTADLKN------------LELHWDIKMDHEDALDDGDN 747
            S LN L  LRG L IKGL+ CTT DLKN            L+L W+  +   +     +N
Sbjct: 301  SELNELARLRGSLLIKGLELCTTTDLKNAKYMEEKFGIQKLKLRWNRDLYDAETDYASEN 360

Query: 748  DDEGVLEGLKPHSNIRKMIIKGYRGMKLCDWFSSNFLGGLVSIELSHCEKLEHLPQFDQF 807
            DDE VL+ LKPHSN+ KM I+GYRG+KLC+W S ++LGGLV+IEL  CEKL+HLPQFDQF
Sbjct: 361  DDERVLDCLKPHSNVHKMQIRGYRGVKLCNWLSFDYLGGLVNIELQSCEKLQHLPQFDQF 420

Query: 808  LYLKHLLLGYLPNIEYIDSGNSVSSSTTFFPSLEKLRIESMPKLKGWWKGEIS------- 867
             +LKHLLL  LP+IEYID+ NS+SSS TFFPSLEKL I +MP LKGWWKGE         
Sbjct: 421  PFLKHLLLENLPSIEYIDNNNSLSSS-TFFPSLEKLTIMTMPNLKGWWKGETPPESARYS 480

Query: 868  --FPTTILHQLSELCIFYCPLLASIPQHPSLESLRICGVSVQLFQMVIRMATDLSEHSSS 927
              FP TILH LS L I  CP LASIPQHP L SL +  VSVQLF MVI+MAT  +  + S
Sbjct: 481  ALFP-TILHHLSRLDISNCPQLASIPQHPPLRSLALNDVSVQLFDMVIKMAT--TPAADS 540

Query: 928  SSTLSKLSFLEIGTIDLEFLPVELFCNMTHLESLIIERCKSLQMSSPHPVDEDND-VVWK 987
            SS LSKLS L I  IDLEFLP ELF + T LE   +  CK+LQMSS H VDEDND V+ K
Sbjct: 541  SSALSKLSILHIQNIDLEFLPEELFGSTTDLEIFTVVNCKNLQMSSSHLVDEDNDGVLGK 600

Query: 988  KLSNLRTLRLESILKLEYFPKSLKYITSLETLKLSNCENLVSTEGIGELISLSHLEIDRC 1047
            KL NL +L +  + +LEY  K LKY+T+LE L L NC N+VS EGI  L SLS L I  C
Sbjct: 601  KLGNLHSLGIFDMPQLEYLWKELKYMTTLERLDLYNCPNIVSLEGISHLTSLSSLRICNC 660

Query: 1048 PNLPILSEDVGDLISLSHLLIWNCPKLTSLSEGITRLTSLSSLCLEDCPNLVSLPQEFLH 1103
             NL  L E +  L SLS+L I  CP LTSL  GI  LTSLS+L ++ C NL SLP E + 
Sbjct: 661  SNLTSLPEGISHLTSLSYLTIVCCPNLTSLPAGIGHLTSLSTLLIKYCVNLTSLP-EGVS 720

BLAST of Cucsa.128100 vs. TrEMBL
Match: A0A0A0K5Z6_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_7G425920 PE=4 SV=1)

HSP 1 Score: 103.2 bits (256), Expect = 2.1e-18
Identity = 80/204 (39.22%), Postives = 102/204 (50.00%), Query Frame = 1

Query: 906  STLSKLSFLEIGTIDLEFLPVELFCNMTHLESLIIERCKSLQMSSPHPVDEDNDVVWKKL 965
            +TL +L       I    + +E   ++T L SL I  C +L  S P  +          L
Sbjct: 615  TTLERLDLYNCPNI----VSLEGISHLTSLSSLRICNCSNLT-SLPEGISH--------L 674

Query: 966  SNLRTLRLESILKLEYFPKSLKYITSLETLKLSNCENLVST-EGIGELISLSHLEIDRCP 1025
            ++L  L +     L   P  + ++TSL TL +  C NL S  EG+  L SLS   I+ CP
Sbjct: 675  TSLSYLTIVCCPNLTSLPAGIGHLTSLSTLLIKYCVNLTSLPEGVSHLTSLSSFTIEECP 734

Query: 1026 NLPILSEDVGDLISLSHLLIWNCPKLTSLSEGITRLTSLSSLCLEDCPNLVSLPQEFLHH 1085
             L  L E V  L SLS L+I  C  LTSL EGI  LTSLS   +E+C NL SLP+  L H
Sbjct: 735  CLTSLPEGVSHLTSLSTLIIRRCVNLTSLPEGIGHLTSLSIFTIEECLNLTSLPEGLL-H 794

Query: 1086 HSSLPGGRFLRILNCPKLQIQDKK 1109
             SSL G   L +  CPKL    KK
Sbjct: 795  LSSLRGS--LTVSKCPKLSKTWKK 802


HSP 2 Score: 50.4 bits (119), Expect = 1.6e-02
Identity = 34/103 (33.01%), Postives = 57/103 (55.34%), Query Frame = 1

Query: 572 NLFQLRTLNLDRCCCHPPKFVDKLKHLRYLNLSGLN----VTFLPNSITTLYNLETLILR 631
           +L  L TL L + C +     + + HL  L+   +     +T LP  ++ L +L TLI+R
Sbjct: 684 HLTSLSTL-LIKYCVNLTSLPEGVSHLTSLSSFTIEECPCLTSLPEGVSHLTSLSTLIIR 743

Query: 632 YCLWLRKLPKDINNLINLRHLDIYDCSSLTHMPKGLGGMTSLQ 671
            C+ L  LP+ I +L +L    I +C +LT +P+GL  ++SL+
Sbjct: 744 RCVNLTSLPEGIGHLTSLSIFTIEECLNLTSLPEGLLHLSSLR 785


HSP 3 Score: 733.4 bits (1892), Expect = 4.1e-208
Identity = 468/1217 (38.46%), Postives = 678/1217 (55.71%), Query Frame = 1

Query: 1    MADSVLFNVAASVITKLGSSALRELGSLWGVNDELDKLQNTLSAIKAVLLDAEEQQSKSH 60
            M ++VL ++   ++ KLG+ AL+E+G +WGV DEL+KL+NT S IKA+ LDAEEQQ+KSH
Sbjct: 1    MTEAVLVSITDRILGKLGNLALQEIGLIWGVKDELEKLRNTASVIKAIFLDAEEQQTKSH 60

Query: 61   TVKDWIAKIKDVFYDIDDLIDEFSYETLRRQVLTKDRTITKQVRIFFSKSNQIAFGFKMG 120
             V+DW+ K+KD  YD DDL+D+FS E L++Q++ +D+    +V  FFSK  + A+GF M 
Sbjct: 61   EVRDWLQKLKDAIYDADDLLDDFSTEMLQQQLMMQDQKAI-EVCAFFSKIKKTAYGFSMS 120

Query: 121  QTIKKVREKLDAIAAIKAQLHLSVCAREVRDNEPRKVRETSSFIPEGEIIGRDEDRKSVM 180
              IK +RE+L+ IA+ +++ HL+   R++      +  +T SF+   E++GR++D+ +++
Sbjct: 121  CKIKAIRERLNDIASDRSKFHLTDHPRQMPSVIAER-EQTHSFVCVEEVVGREDDKLAIV 180

Query: 181  DFLLNTSNITKDNVEVVSIVGMGGLGKTALAQTVYNDEKINNRFKWKIWVCISQEFDIKV 240
            + LL+++  T++NV V+ +VG+GGLGKT L Q VYN EKI   F+ +IWVC+S  FD+K+
Sbjct: 181  ELLLHSN--TEENVSVIPVVGIGGLGKTTLVQLVYNSEKIRRHFELRIWVCVSDVFDVKL 240

Query: 241  IVEKILESITKTKQESLQLDILQSMLQEKIYGKKYLLVMDDVWNVDHEKWIGLKRFLMGG 300
            IV+KILES T TK + L++D L + L ++I GKK+LL++DDVWN + E+W+ L+  LMGG
Sbjct: 241  IVQKILESATNTKCDGLEMDSLLTRLGKEIDGKKFLLILDDVWNDNRERWLKLRDLLMGG 300

Query: 301  ASGSKILVTTRNLQTAQASDTVWFHHLKELDKDNSWALFRKMAFLNKEEELENSNLVRIG 360
            A GSK++VTTR    A  + T   + L+ L +D SW+LF K+AF  + ++ EN+ LV IG
Sbjct: 301  ARGSKVVVTTRTQLIATITGTAKPYFLRSLSEDESWSLFEKLAF-KQGKDFENTRLVAIG 360

Query: 361  KEIVAKLKGYPLSIRVVGRLLYFKNTEMDWSSFKDNELDSILQEDDQIQPILKISFNHLP 420
            KE+V K  G PL+IR +G LLY K+TE +W SFKD +L  I Q ++ I PILK+S+  LP
Sbjct: 361  KEVVKKCAGVPLAIRTMGSLLYCKDTETEWLSFKDRDLSMIPQNENDILPILKLSYELLP 420

Query: 421  PKLKQCFTYCALFPKDYEFKKNGLVKQWMAQGFIQ-AHNKKAIEDVGDDYFQELVGRSFF 480
            P LK CF YC+LFPKDYE  K  L+K WMAQGF+Q A   +  E+VG   F +L  RSFF
Sbjct: 421  PCLKNCFAYCSLFPKDYEINKQTLIKLWMAQGFLQPADGMQHPEEVGHQCFMDLARRSFF 480

Query: 481  QDIRKNKWGDLKYCKMHDLLHDLACSIGENECVVVSDDVGSIDKRTRHASFLLSKRLTRE 540
            QD+   +WGD+  C MHDL+HDLA  +G +E   V  +  +I +R RH S L  +  + +
Sbjct: 481  QDLEYGEWGDVVSCGMHDLMHDLALLVGGSESSTVDSNAENISERIRHVS-LDFELDSSQ 540

Query: 541  VVSKSSIEVTSLRTLDIDSRASFRS-FKKTCHMNLFQ----LRTLNLDRCCCH-PPKFVD 600
             +  S  +   +RT  +  +  +R    +  H  +      LR L+         P  + 
Sbjct: 541  KIPPSLFKANKIRTFVLPVQPVYRKILNQAPHDTIISSFRCLRALDFHNTGVDIVPSSIS 600

Query: 601  KLKHLRYLNLS-GLNVTFLPNSITTLYNLETLILRYCLWLRKLPKDINNLINLRHLDIYD 660
            KLKHLRYL+LS   ++  LP  I  L NL+TL L  C  L  LP+ I+ +I+LRHL+I  
Sbjct: 601  KLKHLRYLDLSKNEDLKRLPRCIARLKNLQTLKLSSCKRLEALPRHISKMISLRHLEIDQ 660

Query: 661  CSSLTHMPKGLGGMTSLQTMSMFVLGK-NKGGDLSA----LNGLKSLRGLLCIKGLQFCT 720
            C+ LTHMP GLG +T+LQT++ FV+GK     DLSA    LNGL  LRG   I  L+   
Sbjct: 661  CTGLTHMPNGLGQLTALQTLTQFVVGKYGSSPDLSARLRELNGLNDLRGEFKISKLEKLK 720

Query: 721  TA-------------DLKNLELHWDIKMDHEDALDDGDNDDEGVLEGLKPHSNIRKMIIK 780
             +             +L+ L L W   ++ +  +D    +DE +LE  +PHSN+++  I 
Sbjct: 721  VSATESREANLKGKENLEVLRLEWTRGVNDDRVID----EDEVLLESFQPHSNLKEFHIY 780

Query: 781  GYRGMKLCDWFSSN---FLGGLVSIELSHCEKLEHLPQFDQFLYLKHLLLGYLPNIEYID 840
            GYR  K   W   N    L  L  I +  C +   LP F Q   LK L L  +  +EYI+
Sbjct: 781  GYRAGKFPSWMVLNLSLLLPNLQEIIIWRCYRCLELPMFSQLPMLKVLKLEEVTALEYIE 840

Query: 841  SGNSVSS--------STTFFPSLEKLRIESMPKLKGWWKGE------------------- 900
            + ++ SS        S  FFPSL++LR+  +   KGWW+ E                   
Sbjct: 841  NSSNGSSSLGKREEKSALFFPSLQELRLFDLRNFKGWWREEVSVVNNDEATVETTTETAG 900

Query: 901  ISFPTTI-------LHQ---------LSELCIFYCPLLASIPQHPSLESLRICGVSVQLF 960
            IS P+         L Q         LS+L I +CP L+++P HP L  +    V+  L 
Sbjct: 901  ISLPSVAACEKQQPLQQQLVLPSFPCLSKLTIGHCPNLSNLPLHPFLNEVEFKDVNAGLV 960

Query: 961  QMVIRMATDLSEHSSSSSTLSKLSF---LEI------GTIDLEFLPVELFCNMTHLESLI 1020
            Q  +     +   S+S    S  SF   L++        +DL  +      N+T+LE L 
Sbjct: 961  QWSMVGLASIEGSSASGRNTSLPSFPSTLKLKHLCMDSVMDLVSISELGLQNLTYLEHLT 1020

Query: 1021 IERCKSLQMSSPHPVDEDNDVVWKKLSNLRTLRLESIL---KLEYFPKSLKYITSLETLK 1080
            IE C +L  S P           + L  LR+LR  SI     L      L+Y+TSLE L+
Sbjct: 1021 IENCPNLS-SLPE----------ESLRGLRSLRFLSIRGCGSLTSLFLGLQYLTSLEELE 1080

Query: 1081 LSNCENLVSTEGIGE-------LISLSHLEIDRCPNLPILSEDVGDLISLSHLLIWNCPK 1127
            +  C  L  ++   E       + SL  L+I   P L  + + + ++ SL  L I  C  
Sbjct: 1081 IEECRALDMSDCDEENSLQFRGMKSLRRLKIGYMPQLESIPDGIHEVTSLQDLKIEGCVG 1140

BLAST of Cucsa.128100 vs. TrEMBL
Match: A0A0D2NDD9_GOSRA (Uncharacterized protein OS=Gossypium raimondii GN=B456_005G155200 PE=3 SV=1)

HSP 1 Score: 729.2 bits (1881), Expect = 7.8e-207
Identity = 455/1177 (38.66%), Postives = 659/1177 (55.99%), Query Frame = 1

Query: 1    MADSVLFNVAASVITKLGSSALRELGSLWGVNDELDKLQNTLSAIKAVLLDAEEQQSKSH 60
            MA++ LFN+A  ++ K+G+ AL+E+G +WGV ++L+KL+NT+S IKAVLLDAE+Q +KSH
Sbjct: 1    MAEAGLFNIADGILGKIGNLALQEIGLVWGVKEQLEKLKNTVSTIKAVLLDAEDQHAKSH 60

Query: 61   TVKDWIAKIKDVFYDIDDLIDEFSYETLRRQVLTKDRTITKQVRIFFSKSNQIAFGFKMG 120
             V+DW+ K+KD  YD DDL+D FS   L+RQ   +     KQV   FSK +Q+ +  K+ 
Sbjct: 61   EVRDWLGKLKDAVYDTDDLLDNFSTHVLKRQQGKRG----KQVSFLFSKVSQVTYNLKIS 120

Query: 121  QTIKKVREKLDAIAAIKAQLHLSVCAREVRDNEPRKV-------RETSSFIPEGEIIGRD 180
              I+ +REKLDAIAA K + H +       D  P  +       R+T SF+ + +++GR 
Sbjct: 121  HQIEAIREKLDAIAADKIKYHFT-------DRSPISIPLVKVERRQTHSFVRKEDVVGRQ 180

Query: 181  EDRKSVMDFLLNTSNITKDNVEVVSIVGMGGLGKTALAQTVYNDEKINNRFKWKIWVCIS 240
             D+ ++M  LL ++ +  DNV V+ IVG+GG GKT LAQ  YNDE+    F+ ++WVC+S
Sbjct: 181  GDKDAIMKRLLESNGV--DNVSVIPIVGIGGQGKTTLAQLAYNDERTVKHFELRMWVCVS 240

Query: 241  QEFDIKVIVEKILESITKTKQESLQLDILQSMLQEKIYGKKYLLVMDDVWNVDHEKWIGL 300
              FD+K+IVEKILES T +K ESL++D LQ+ L+++I G+KYLLV+DD+WN   E+W+ L
Sbjct: 241  DVFDVKMIVEKILESATSSKYESLEMDSLQTHLRKRIDGRKYLLVLDDMWNDSRERWLNL 300

Query: 301  KRFLMGGASGSKILVTTRNLQTAQASDTVWFHHLKELDKDNSWALFRKMAFLNKEEELEN 360
               LM GA GSKI+VTTR    A  + T   + L+ L +D SW+LF K+AF  + +E  +
Sbjct: 301  ADLLMNGARGSKIIVTTRAQLVASITGTSQPYLLEGLPEDMSWSLFEKVAF-KESKETND 360

Query: 361  SNLVRIGKEIVAKLKGYPLSIRVVGRLLYFKNTEMDWSSFKDNELDSILQEDDQIQPILK 420
            S LV IGK+IV K  G PL IR +G +LY K+TE +W S K+ +L  + + +D +  +LK
Sbjct: 361  SRLVAIGKDIVKKCAGNPLVIRTIGGVLYTKDTETEWLSLKERQLSMLTRNEDDVLSVLK 420

Query: 421  ISFNHLPPKLKQCFTYCALFPKDYEFKKNGLVKQWMAQGFIQ-AHNKKAIEDVGDDYFQE 480
            +S+  LP  LKQCF YC+LFPKDYE  K  L+  W+A+GFIQ     + +E++G  YF +
Sbjct: 421  LSYEQLPSYLKQCFAYCSLFPKDYEINKQMLISLWIAEGFIQPLQGIQCLEELGGQYFMD 480

Query: 481  LVGRSFFQDIRKNKWGDLKYCKMHDLLHDLACSIGENECVVVSDDVGSIDKRTRHASFLL 540
            L+ RSFFQD+  ++WG++  CK+HDL+HDLA  I  ++C +V  D  +I +RT H S  L
Sbjct: 481  LLRRSFFQDVEYDEWGNVISCKLHDLMHDLAQLIAGSDCSMVDLDCENISERTHHVS--L 540

Query: 541  SKRLTRE-VVSKSSIEVTSLRTLDIDSRASFR-SFKKTCHMNLFQ----LRTLNLDRCCC 600
            S  L     +  + +    +RT  +  +   R    K  H  +      +R L+L     
Sbjct: 541  SAELDSSWKIPTTLLNANKIRTFLLPMQPIHRVVLDKVDHEAIISSFRLMRVLDLHNTGL 600

Query: 601  H-PPKFVDKLKHLRYLNLSGLNV-TFLPNSITTLYNLETLILRYCLWLRKLPKDINNLIN 660
            H  P+ + KLKHLRYL+LS   V   LP+SIT L NL+TL +  C  L +LP+ ++N+I+
Sbjct: 601  HILPRIIGKLKHLRYLDLSKNEVIRKLPSSITELLNLQTLKIYSCKRLEQLPRKLSNMIS 660

Query: 661  LRHLDIYDCSSLTHMPKGLGGMTSLQTMSMFVLGKNK----GGDLSALNGLKSLRGLLCI 720
            L+HL+   C+ LTHMP G+G +TSLQT++ FV+G +      G L  L  L  LRG L I
Sbjct: 661  LKHLETGQCTGLTHMPSGIGQLTSLQTLTRFVVGMSSFEMASGGLRELKDLNELRGELMI 720

Query: 721  KGLQ-------FCTTADLKN------LELHWDIKMDHEDALDDGDNDDEGVLEGLKPHSN 780
              L+        C  A+LK       L L W  ++++  + +    +DE +LEGL+PHSN
Sbjct: 721  AKLENLRNVAAECEEANLKEKQHLEVLTLDWSREVNNHVSFE----EDEALLEGLQPHSN 780

Query: 781  IRKMIIKGYRGMKLCDWFSSN---FLGGLVSIELSHCEKLEHLPQFDQFLYLKHLLLGYL 840
            +++  I GYR  +   W S +    L  L+ I + +C K  HLP F Q   LK L L  +
Sbjct: 781  LQEFHIYGYRAERFPKWMSFDMALLLPNLLEITIWNCIKCIHLPLFSQLPKLKVLRLEVI 840

Query: 841  PNIEYIDSGNSVSSST---------------TFFPSLEKLRIESMPKLKGWWK------- 900
              +EYI+   + SSS+                FFP LE+L    +  LKGWW+       
Sbjct: 841  TAVEYIEDSRAESSSSLSFKGNPMNRGREGKEFFPCLEELVFFDLRNLKGWWREAPPVTN 900

Query: 901  ---GEISFPTTILHQ---------LSELCIFYCPLLASIPQHPSLESLRICGVSVQLFQM 960
               G  + P   L +         LS+L I  C  L  +P HP LE L +     +L Q 
Sbjct: 901  NNHGAAASPQRPLQKKESMVSFPCLSKLKIGICTNLTHMPLHPFLEELELKNTPARLLQQ 960

Query: 961  VIRMATDLSEHSSSSSTLSKLSFLEI-GTIDLEFLPVELFCNMTHLESLIIERCKSLQMS 1020
               +A     +      LSKL  + I G IDL   P +   ++T L+ L IE C  L   
Sbjct: 961  SAMVAA--GANLVYPLYLSKLKVMHIDGIIDLVSFPEKGIHHLTSLQHLSIENCPDLVCL 1020

Query: 1021 SPHPVDEDNDVVWKKLSNLRTLRLESILKLEYFPKSLKYITSLETLKLSNCENLVST--- 1080
            +   +        K L++LR   +     L+   K  K++T+LE L++  C  L  +   
Sbjct: 1021 TEEGL--------KSLTSLRFFNIRCCEILKSLFKGFKHLTALEELEIKECRELDLSKDL 1080

Query: 1081 -EGIGELISLSHLEIDRCPNLPILSEDVGDLISLSHLLIWNCPKLTSLSEGITRLTSLSS 1103
             E + EL  L  L I+  P L  L + +  + +L  L I +C  L +L E I  LTSL  
Sbjct: 1081 EENVMELQCLRTLRIEDMPKLSSLPDGLQHVTTLKDLQISSCSNLKTLPEWIGNLTSLQR 1140

BLAST of Cucsa.128100 vs. TrEMBL
Match: A0A0A0KIT2_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_6G375730 PE=3 SV=1)

HSP 1 Score: 726.1 bits (1873), Expect = 6.6e-206
Identity = 412/759 (54.28%), Postives = 524/759 (69.04%), Query Frame = 1

Query: 362  EIVAKLKGYPLSIRVVGRLLYFKNTEMDWSSFKDNELDSILQEDDQIQPILKISFNHLPP 421
            EIV KLKG PL+IRV+G  LY K +E DW SFKD+ELD+I+Q++++IQ ILKISFNHL  
Sbjct: 196  EIVTKLKGSPLAIRVIGSYLYSKKSEKDWLSFKDHELDTIMQQENEIQSILKISFNHLSS 255

Query: 422  KLKQCFTYCALFPKDY--EFKKNGLVKQWMAQGFIQAHNKKAIEDVGDDYFQELVGRSFF 481
             LK CFTYCALF KDY  E +KN L+KQWMAQGFIQ HNKKA+EDVGDDYF+EL+GRSFF
Sbjct: 256  SLKHCFTYCALFSKDYHHEIRKNDLIKQWMAQGFIQPHNKKAMEDVGDDYFEELLGRSFF 315

Query: 482  QDIRKNKWGDLKYCKMHDLLHDLACSIGENECVVVSDDVGSIDKRTRHASFLLSKRLTR- 541
            QDIRKNKWG++   KMHD++HDLACS+ EN+CV+ +DD  SIDKRTRH S      +TR 
Sbjct: 316  QDIRKNKWGEINKFKMHDIIHDLACSVVENDCVLANDDTKSIDKRTRHVSISAFNSMTRW 375

Query: 542  EVVSKSSIEVTSLRTLDIDSRASFRSFKKTCHMNL---FQLRTLNLDRCCCHPPKFVDKL 601
            ++++KS IE  +LRTL         ++ +  H++L    +LRTLNL+      PK + K+
Sbjct: 376  KLITKSLIEAKNLRTL---------NYARRHHIDLSNHLRLRTLNLE--FHFVPKCIGKM 435

Query: 602  KHLRYLNLSGLNVTFLPNSITTLYNLETLILRYCLWLRKLPKDINNLINLRHLDIYDCSS 661
            KHLRY+N++   + FLP ++T LY+LETLI+R CL LR+L  DI NLINLRHLDI D   
Sbjct: 436  KHLRYINITYCYIDFLPKAVTKLYHLETLIIRGCLELRELSSDIKNLINLRHLDIKDFKH 495

Query: 662  L-THMPKGLGGMTSLQTMSMFVLGKNKGGDLSALNGLKSLRGLLCIKGLQFCTTADLKN- 721
            + ++MPKG+G MT+LQTM++F+LG+NKGG+LS LNGL +LRG L I+ LQFC    L+N 
Sbjct: 496  VWSYMPKGMGSMTTLQTMNLFILGENKGGELSELNGLVNLRGSLSIQQLQFCKPIGLENV 555

Query: 722  -----------LELHWDIKMDHEDALDDGDNDDEGVLEGLKPHSNIRKMIIKGYRGMKLC 781
                       LELHW      E  +DD   +DE VLE LKPHSN++K+ I+GYRG+KLC
Sbjct: 556  KYLEEKSRIQKLELHWK-TYQRESKIDD---EDERVLESLKPHSNLQKIRIEGYRGLKLC 615

Query: 782  DWFSSNFLGGLVSIELSHCEKLEHLPQFDQFLYLKHLLLGYLPNIEYIDSGNSVSSS-TT 841
            +WFS + +  LV I+L +CEKL+ LP+FD+F +LKHL L  LP+IEYI   N VSSS TT
Sbjct: 616  NWFSFDSIVNLVFIKLFNCEKLQQLPRFDRFPFLKHLHLEDLPSIEYIAINNYVSSSMTT 675

Query: 842  FFPSLEKLRIESMPKLKGWWKGE-----ISFPTTILHQLSELCIFYCPLLASIPQHPSLE 901
            FFPSLE L I  +P LK WWKGE      SFP TIL  LS+L I YC  LASIPQH  L+
Sbjct: 676  FFPSLENLSIIKLPNLKEWWKGESIDQNTSFP-TILRHLSQLKIHYCRQLASIPQHGPLQ 735

Query: 902  SLRICGVSVQLFQMVIRM-ATDL--SEHSSSSST---LSKLSFLEIGTIDLEFLPVELFC 961
            SL I  +S+QLF++VI+M AT++   E SSSS+    +   S L+I  ID EFLP +LF 
Sbjct: 736  SLDIRDISLQLFELVIKMTATNIIVGEDSSSSANDMFIRSSSSLKIWKIDWEFLPNDLFS 795

Query: 962  NMTHLESLIIERCKSLQMSSPHPVDEDNDVVWKKLSNLRTLRLESILKLEYFPKSLKYIT 1021
            N+THL+SL+I RC +L+MS      +D++V WK+L +LRTLRL  I KLEY PK  +Y+ 
Sbjct: 796  NVTHLQSLVIGRCFNLKMSF-----DDDNVRWKELGSLRTLRLCFIPKLEYLPKGFQYLK 855

Query: 1022 SLETLKLSNCENLVSTEGIGELISLSHLEIDRCPNLPILSEDVGDLISLSHLLIWNCPKL 1081
            +LE L+L  CENL    GI  L SLS LEI                         NCP L
Sbjct: 856  ALEHLELLWCENLACILGIEHLTSLSRLEIS------------------------NCPNL 908

Query: 1082 TSLSEGITRLTSLSSLCLEDCPNLVSLPQEFLHHHSSLP 1090
            TSL EG+T+L SL+ L ++DCPNL +LP E LHH  + P
Sbjct: 916  TSLPEGMTQLISLTCLIIDDCPNLSTLP-EGLHHLLNTP 908

BLAST of Cucsa.128100 vs. TAIR10
Match: AT3G14470.1 (AT3G14470.1 NB-ARC domain-containing disease resistance protein)

HSP 1 Score: 431.0 bits (1107), Expect = 2.2e-120
Identity = 315/1011 (31.16%), Postives = 515/1011 (50.94%), Query Frame = 1

Query: 35   LDKLQNTLSAIKAVLLDAEEQQSKSHTVKDWIAKIKDVFYDIDDLIDEFSYETLRRQVLT 94
            L++L   L  I AVL+DAEE+Q  +  V+ W+ +++DV Y  +D +D+ + E LR  +  
Sbjct: 39   LERLSTALLTITAVLIDAEEKQITNPVVEKWVNELRDVVYHAEDALDDIATEALRLNIGA 98

Query: 95   KDRTITK--QVRIFFSKSNQIAFGFKMGQT-IKKVREKLDAIAAIKAQLHLSVCAREVRD 154
            +  +  +  Q+R   S  + +    +  +T ++KV  +L+ +A+ +  L L    +E+  
Sbjct: 99   ESSSSNRLRQLRGRMSLGDFLDGNSEHLETRLEKVTIRLERLASQRNILGL----KELTA 158

Query: 155  NEPRKVRETSSFIPEGEIIGRDEDRKSVMDFLLNTSNITKDN-VEVVSIVGMGGLGKTAL 214
              P++   T+S + E E+ GRD+D+  +M FL+  +   KDN + VV+IVG+GG+GKT L
Sbjct: 159  MIPKQRLPTTSLVDESEVFGRDDDKDEIMRFLIPENG--KDNGITVVAIVGIGGVGKTTL 218

Query: 215  AQTVYNDEKINNRFKWKIWVCISQEFDIKVIVEKILESITKTKQESLQLDILQSMLQEKI 274
            +Q +YND+ + + F  K+W  +S+EFD+  I +K+ ES+T    E   LD+LQ  L+E++
Sbjct: 219  SQLLYNDQHVRSYFGTKVWAHVSEEFDVFKITKKVYESVTSRPCEFTDLDVLQVKLKERL 278

Query: 275  YGK--KYLLVMDDVWNVDHEKWIGLKRFLMGGASGSKILVTTRNLQTAQASDTVWFHHLK 334
             G    +LLV+DD+WN +   W  L++  +  A GS+ILVTTR+ + A     V  H+L+
Sbjct: 279  TGTGLPFLLVLDDLWNENFADWDLLRQPFIHAAQGSQILVTTRSQRVASIMCAVHVHNLQ 338

Query: 335  ELDKDNSWALFRKMAFLNKEEELENSNLVRIGKEIVAKLKGYPLSIRVVGRLLYFKNTEM 394
             L   + W+LF K  F N+E  L N  +  + + IV K +G PL+++ +G +L F+   +
Sbjct: 339  PLSDGDCWSLFMKTVFGNQEPCL-NREIGDLAERIVHKCRGLPLAVKTLGGVLRFEGKVI 398

Query: 395  DWSSFKDNELDSILQEDDQIQPILKISFNHLPPKLKQCFTYCALFPKDYEFKKNGLVKQW 454
            +W     + +  +  +   + P+L++S+ +LP  LK+CF YC++FPK + F+K+ +V  W
Sbjct: 399  EWERVLSSRIWDLPADKSNLLPVLRVSYYYLPAHLKRCFAYCSIFPKGHAFEKDKVVLLW 458

Query: 455  MAQGFI-QAHNKKAIEDVGDDYFQELVGRSFFQDIRKNKWGDLKYCKMHDLLHDLA-CSI 514
            MA+GF+ Q  + K +E++G++YF EL  RS  Q  +       +Y  MHD +++LA  + 
Sbjct: 459  MAEGFLQQTRSSKNLEELGNEYFSELESRSLLQKTK------TRYI-MHDFINELAQFAS 518

Query: 515  GENECVVVSDDVGSIDKRTRHASFLLSKRLTREVVSKSSIEVTSLRT-LDIDSRASFRS- 574
            GE            + +RTR+ S+ L       +  ++  EV  LRT L +    S RS 
Sbjct: 519  GEFSSKFEDGCKLQVSERTRYLSY-LRDNYAEPMEFEALREVKFLRTFLPLSLTNSSRSC 578

Query: 575  -----FKKTCHMNLFQLRTLNLD--RCCCHPPKFVDKLKHLRYLNLSGLNVTFLPNSITT 634
                   +     L +LR L+L   +    PP F   + H R+L+LS   +  LP S+  
Sbjct: 579  CLDQMVSEKLLPTLTRLRVLSLSHYKIARLPPDFFKNISHARFLDLSRTELEKLPKSLCY 638

Query: 635  LYNLETLILRYCLWLRKLPKDINNLINLRHLDIYDCSSLTHMPKGLGGMTSLQTMSMFVL 694
            +YNL+TL+L YC  L++LP DI+NLINLR+LD+   + L  MP+  G + SLQT++ F +
Sbjct: 639  MYNLQTLLLSYCSSLKELPTDISNLINLRYLDLIG-TKLRQMPRRFGRLKSLQTLTTFFV 698

Query: 695  GKNKGGDLSALNGLKSLRGLLCIKGLQ-FCTTAD-----------LKNLELHW--DIKMD 754
              + G  +S L GL  L G L I  LQ     AD           L+ ++  W       
Sbjct: 699  SASDGSRISELGGLHDLHGKLKIVELQRVVDVADAAEANLNSKKHLREIDFVWRTGSSSS 758

Query: 755  HEDALDDGDNDDEGVLEGLKPHSNIRKMIIKGYRGMKLCDWFSSNFLGGLVSIELSHCEK 814
              +       ++  V E L+PH +I K+ I+ Y+G +  DW S      +V I L  C+ 
Sbjct: 759  ENNTNPHRTQNEAEVFEKLRPHRHIEKLAIERYKGRRFPDWLSDPSFSRIVCIRLRECQY 818

Query: 815  LEHLPQFDQFLYLKHLLLGYLPNIE------YIDSGNSVSSSTTFFPSLEKLRIESMPKL 874
               LP   Q   LK L +  +  ++      Y             F SLE LR +++P  
Sbjct: 819  CTSLPSLGQLPCLKELHISGMVGLQSIGRKFYFSDQQLRDQDQQPFRSLETLRFDNLPDW 878

Query: 875  KGWWKGEISFPTTILHQLSELCIFYCP-LLASIPQH-PSLESLRICGVSVQLFQMVIRMA 934
            + W    ++    +   L +L I  CP L  ++P   PSL SL I    +  FQ      
Sbjct: 879  QEWLDVRVT-RGDLFPSLKKLFILRCPELTGTLPTFLPSLISLHIYKCGLLDFQP----- 938

Query: 935  TDLSEHSSSSSTLSKLSFLEIGTIDLEFLPVELFCNMTHLESLIIERCK---SLQMSSPH 994
                 H  S   L  LS ++     L   P+  F N+  LE   +++C    SL++S+ H
Sbjct: 939  ---DHHEYSYRNLQTLS-IKSSCDTLVKFPLNHFANLDKLE---VDQCTSLYSLELSNEH 998

Query: 995  PVDEDNDVVWKKLSNLRTLRLESILKLEYFPKSLKYITSLETLKLSNCENL 1004
                      +  + LR LR+     L+  PK L  +     + ++NC  L
Sbjct: 999  ---------LRGPNALRNLRINDCQNLQLLPK-LNALPQNLQVTITNCRYL 1010

BLAST of Cucsa.128100 vs. TAIR10
Match: AT3G14460.1 (AT3G14460.1 LRR and NB-ARC domains-containing disease resistance protein)

HSP 1 Score: 369.0 bits (946), Expect = 1.0e-101
Identity = 278/903 (30.79%), Postives = 435/903 (48.17%), Query Frame = 1

Query: 1   MADSVLFNVAASVITKLGSSA-LRELGSLWGVNDELDKLQNTLSAIKAVLLDAEEQQSKS 60
           MA+S L + A  ++ ++ +S  L EL      +  L +L+  L     VL DA+++    
Sbjct: 1   MANSYLSSCANVMVERINTSQELVELCKGKSSSALLKRLKVALVTANPVLADADQRAEHV 60

Query: 61  HTVKDWIAKIKDVFYDIDDLIDEFSYETLRRQVLTKDRTITKQVRIFFSKSNQIAFGFKM 120
             VK W+  IKD F+  +D++DE   E LRR+V+ +   +    +   +    I      
Sbjct: 61  REVKHWLTGIKDAFFQAEDILDELQTEALRRRVVAEAGGLGGLFQNLMAGREAIQ----- 120

Query: 121 GQTIKKVREKLDAIAAIKAQ--LHLSVCA----REVRDNEPRKV-RETSSFIPEGEIIGR 180
               KK+  K++ +  +      H+ V       E R+ + R+  R     +P+G ++GR
Sbjct: 121 ----KKIEPKMEKVVRLLEHHVKHIEVIGLKEYSETREPQWRQASRSRPDDLPQGRLVGR 180

Query: 181 DEDRKSVMDFLLNTSNITKDNVEVVSIVGMGGLGKTALAQTVYNDEKINNRFKWKIWVCI 240
            ED+ ++++ LL+   I+     V+S+VGM G+GKT L + V+ND ++   F+ K+W+  
Sbjct: 181 VEDKLALVNLLLSDDEISIGKPAVISVVGMPGVGKTTLTEIVFNDYRVTEHFEVKMWISA 240

Query: 241 SQEFDIKVIVEKILESITKTKQESLQLDILQSMLQEKIYGKKYLLVMDDVWNVDHEKWIG 300
              F++  + + +L+ IT +   +  L  LQ  L++ + GK++LLV+DD W+    +W  
Sbjct: 241 GINFNVFTVTKAVLQDITSSAVNTEDLPSLQIQLKKTLSGKRFLLVLDDFWSESDSEWES 300

Query: 301 LKRFLMGGASGSKILVTTRNLQTAQASDTVWFHHLKELDKDNSWALFRKMAFLNKEEELE 360
            +        GSKI++TTR+   +  +     + +K +  +  W L  + AF N      
Sbjct: 301 FQVAFTDAEEGSKIVLTTRSEIVSTVAKAEKIYQMKLMTNEECWELISRFAFGNISVGSI 360

Query: 361 NSNLVRIGKEIVAKLKGYPLSIRVVGRLLYFKNTEMDWSSFKDNELDSILQEDDQIQPIL 420
           N  L  IGK I  + KG PL+ R +   L  K    DW +   N         + I P+L
Sbjct: 361 NQELEGIGKRIAEQCKGLPLAARAIASHLRSKPNPDDWYAVSKN----FSSYTNSILPVL 420

Query: 421 KISFNHLPPKLKQCFTYCALFPKDYEFKKNGLVKQWMAQGFI-QAHNKKAIEDVGDDYFQ 480
           K+S++ LPP+LK+CF  C++FPK + F +  LV  WMA   + Q  + + +ED+G+DY  
Sbjct: 421 KLSYDSLPPQLKRCFALCSIFPKGHVFDREELVLLWMAIDLLYQPRSSRRLEDIGNDYLG 480

Query: 481 ELVGRSFFQ--DIRKNKWGDLKYCKMHDLLHDLACSIGENECVVVSDD-VGSIDKRTRHA 540
           +LV +SFFQ  DI    +       MHDL++DLA ++  + C  + DD +  I   TRH 
Sbjct: 481 DLVAQSFFQRLDITMTSF------VMHDLMNDLAKAVSGDFCFRLEDDNIPEIPSTTRHF 540

Query: 541 SFLLSKRLTREVVSKSSIEVTSLRT-LDIDSRASFRSFKKTCHM------NLFQLRTLNL 600
           SF  S +    V  +S      LRT L  +S  S  S + T  +       L  LR L+L
Sbjct: 541 SFSRS-QCDASVAFRSICGAEFLRTILPFNSPTSLESLQLTEKVLNPLLNALSGLRILSL 600

Query: 601 DRC-CCHPPKFVDKLKHLRYLNLSGLNVTFLPNSITTLYNLETLILRYCLWLRKLPKDIN 660
                 + PK +  LK LRYL+LS   +  LP  + TL NL+TL+L  C  L  LPK I 
Sbjct: 601 SHYQITNLPKSLKGLKLLRYLDLSSTKIKELPEFVCTLCNLQTLLLSNCRDLTSLPKSIA 660

Query: 661 NLINLRHLDIYDCSSLTHMPKGLGGMTSLQTMSMFVLGKNKGGDLSALNGLKSLRGLLCI 720
            LINLR LD+   + L  MP G+  + SLQ +S FV+G+  G  L  L  L  LRG L I
Sbjct: 661 ELINLRLLDLVG-TPLVEMPPGIKKLRSLQKLSNFVIGRLSGAGLHELKELSHLRGTLRI 720

Query: 721 KGLQ---FCTTAD---------LKNLELHWDIKMDH--EDALDDGDNDDEGVLEGLKPHS 780
             LQ   F + A          L  L L W +K       + +    D + VL  L+PH 
Sbjct: 721 SELQNVAFASEAKDAGLKRKPFLDGLILKWTVKGSGFVPGSFNALACDQKEVLRMLEPHP 780

Query: 781 NIRKMIIKGYRGMKLCDWFSSNFLGGLVSIELSHCEKLEHLPQFDQFLYLKHLLLGYLPN 840
           +++   I+ Y+G     W   +   G+ S+ LS C     LP   Q   LK+L +     
Sbjct: 781 HLKTFCIESYQGGAFPKWLGDSSFFGITSVTLSSCNLCISLPPVGQLPSLKYLSIEKFNI 840

Query: 841 IEYIDSG---NSVSSSTTFFPSLEKLRIESMPKLKGWWKGEISFPTTILHQLSELCIFYC 867
           ++ +         +S    F SL+ L+   MP+   W   E+     I   L +L I  C
Sbjct: 841 LQKVGLDFFFGENNSRGVPFQSLQILKFYGMPRWDEWICPELE--DGIFPCLQKLIIQRC 880


HSP 2 Score: 62.4 bits (150), Expect = 2.1e-09
Identity = 90/330 (27.27%), Postives = 131/330 (39.70%), Query Frame = 1

Query: 778  LSHCEKLEHLPQFDQFLYLKHLLLGYLPN-------IEYIDSGNSVSSSTTFFPSLEKLR 837
            LS   K      FDQ+       LG LP        I    SG      +T  P + +  
Sbjct: 970  LSSLPKDRQTEDFDQY----ETQLGSLPQQFEEPAVISARYSGYISDIPSTLSPYMSRTS 1029

Query: 838  IESMPKLKGWWKGEISFPTTILHQLSELCIFYCPLLASIPQHPSLESLRICGVSVQLFQM 897
            +   PK +G          +IL   S        + +S+P   S E+++          M
Sbjct: 1030 LVPDPKNEG----------SILPGSSSYQYHQYGIKSSVPSPRSSEAIKPSQYDDDETDM 1089

Query: 898  VIRMATDLSEHSSSSSTLSKLSFLEIGTID-LEFLPVELFCNMTHLESLIIERCKSLQ-M 957
                 TD+S        L  L    I + D L  LP  L  +  +L  L+I  C SL+  
Sbjct: 1090 EYLKVTDISHLMELPQNLQSL---HIDSCDGLTSLPENLTESYPNLHELLIIACHSLESF 1149

Query: 958  SSPHPVDEDNDVV---WKKLSNLRTLR-LESILKLEY------------FPKSLKYITSL 1017
               HP      +     KKL+   +L+   S  +LEY            FP SL     L
Sbjct: 1150 PGSHPPTTLKTLYIRDCKKLNFTESLQPTRSYSQLEYLFIGSSCSNLVNFPLSL--FPKL 1209

Query: 1018 ETLKLSNCENLVSTE---GIGE-LISLSHLEIDRCPNLPILSEDVGDLISLSHLLIWNCP 1077
             +L + +CE+  +     G+G+  I+L  LEI  CPNL    +       LS +L+ NC 
Sbjct: 1210 RSLSIRDCESFKTFSIHAGLGDDRIALESLEIRDCPNLETFPQGGLPTPKLSSMLLSNCK 1269

Query: 1078 KLTSLSEGITRLTSLSSLCLEDCPNLVSLP 1079
            KL +L E +  LTSL SL +  CP + ++P
Sbjct: 1270 KLQALPEKLFGLTSLLSLFIIKCPEIETIP 1280


HSP 3 Score: 35.0 bits (79), Expect = 3.6e-01
Identity = 30/85 (35.29%), Postives = 47/85 (55.29%), Query Frame = 1

Query: 963  KKLSNLRTLRLESILKLEYFPKSLKYITSLETLKLSNCENLVS-TEGIGELISLSHLEID 1022
            K L  LR L L S  K++  P+ +  + +L+TL LSNC +L S  + I ELI+L  L++ 
Sbjct: 593  KGLKLLRYLDLSST-KIKELPEFVCTLCNLQTLLLSNCRDLTSLPKSIAELINLRLLDLV 652

Query: 1023 RCP--NLPILSEDVGDLISLSHLLI 1045
              P   +P   + +  L  LS+ +I
Sbjct: 653  GTPLVEMPPGIKKLRSLQKLSNFVI 676

BLAST of Cucsa.128100 vs. TAIR10
Match: AT1G58602.1 (AT1G58602.1 LRR and NB-ARC domains-containing disease resistance protein)

HSP 1 Score: 265.4 bits (677), Expect = 1.6e-70
Identity = 304/1183 (25.70%), Postives = 520/1183 (43.96%), Query Frame = 1

Query: 14   ITKLGSSALRELGSLWGVNDELDKLQNTLSAIKAVLLDAEEQQSKSHTVKDWIAKIKDVF 73
            + KL      E     GV D++ +L++ L+ +K+ L DA+ ++  S  V+  + +IKD+ 
Sbjct: 10   VNKLWDLLSHEYTLFQGVEDQVAELKSDLNLLKSFLKDADAKKHTSALVRYCVEEIKDIV 69

Query: 74   YDIDDLIDEFSYETLRRQVLTKDRTITKQV-RIFFSKSNQIAFGFKMGQTIKKVREKLDA 133
            YD +D+++ F    ++++ L     I K + R+     ++      +G   K++   +  
Sbjct: 70   YDAEDVLETF----VQKEKLGTTSGIRKHIKRLTCIVPDRREIALYIGHVSKRITRVIRD 129

Query: 134  IAAIKAQLHLSVCAREVRDNEPRKVRETSSFIPEGEIIGRDEDRKSVMDFLLNTSNITKD 193
            + +   Q  +         N  R++R T     E   +  +E+ K ++ +      + +D
Sbjct: 130  MQSFGVQQMIVDDYMHPLRNREREIRRTFPKDNESGFVALEENVKKLVGYF-----VEED 189

Query: 194  NVEVVSIVGMGGLGKTALAQTVYNDEKINNRFKWKIWVCISQEFDIKVIVEKILESITKT 253
            N +VVSI GMGGLGKT LA+ V+N + +  +F    WV +SQ+F +K + + IL  +   
Sbjct: 190  NYQVVSITGMGGLGKTTLARQVFNHDMVTKKFDKLAWVSVSQDFTLKNVWQNILGDLKPK 249

Query: 254  KQESLQ-----LDILQSMLQEKIYG----KKYLLVMDDVWNVDHEKWIGLKRFLMGGASG 313
            ++E+ +     L++ +  LQ ++Y      K L+V+DD+W    E W  +K  +     G
Sbjct: 250  EEETKEEEKKILEMTEYTLQRELYQLLEMSKSLIVLDDIWK--KEDWEVIKP-IFPPTKG 309

Query: 314  SKILVTTRNLQTAQASDTVWFHHLKE-LDKDNSWALFRKMAF-LNKEEELE-NSNLVRIG 373
             K+L+T+RN      ++T +F+   E L  D+SW LF+++AF +N   E E +  + ++G
Sbjct: 310  WKLLLTSRNESIVAPTNTKYFNFKPECLKTDDSWKLFQRIAFPINDASEFEIDEEMEKLG 369

Query: 374  KEIVAKLKGYPLSIRVVGRLLYFKNTEMDWSSFKDNELDSIL--------QEDDQIQPIL 433
            ++++    G PL+I+V+G +L  K T  DW    +N    ++          ++    +L
Sbjct: 370  EKMIEHCGGLPLAIKVLGGMLAEKYTSHDWRRLSENIGSHLVGGRTNFNDDNNNSCNYVL 429

Query: 434  KISFNHLPPKLKQCFTYCALFPKDYEFKKNGLVKQWMAQGFIQA--HNKKAIEDVGDDYF 493
             +SF  LP  LK CF Y A FP+DYE K   L   W A+   Q   ++ + I DVGD Y 
Sbjct: 430  SLSFEELPSYLKHCFLYLAHFPEDYEIKVENLSYYWAAEEIFQPRHYDGEIIRDVGDVYI 489

Query: 494  QELVGRSFFQDIRKNKWGDLKYCKMHDLLHD--LACSIGENECVVVSDDVGSIDKRTRHA 553
            +ELV R+     R  K    + C +HD++ +  L  +  EN   + S+   + + ++   
Sbjct: 490  EELVRRNMVISERDVKTSRFETCHLHDMMREVCLLKAKEENFLQITSNPPSTANFQSTVT 549

Query: 554  SFLLSKRLTREVVSKSSIEVTSLRTLDIDSRASFRSFKKTCHMNLFQLRTLNLDRCCCHP 613
            S  L  +    +  +  I    LR+L + +  S+ +   +    L  LR L+L +     
Sbjct: 550  SRRLVYQYPTTLHVEKDINNPKLRSLVVVTLGSW-NMAGSSFTRLELLRVLDLVQAKLKG 609

Query: 614  PKF---VDKLKHLRYLNLSGLNVTFLPNSITTLYNLETLILRYCLWLRK--LPKDINNLI 673
             K    + KL HLRYL+L    VT +P S+  L  L  L L   L  R   +P  +  + 
Sbjct: 610  GKLASCIGKLIHLRYLSLEYAEVTHIPYSLGNLKLLIYLNLHISLSSRSNFVPNVLMGMQ 669

Query: 674  NLRHLDIYDCSSLTHMPK-GLGGMTSLQTMSMFVLGKNKGGDLSALNGLKSLRGLLCIKG 733
             LR+L +   S +    K  L  +  L+T+  F     K   L  L G+  LR L     
Sbjct: 670  ELRYLAL--PSLIERKTKLELSNLVKLETLENF---STKNSSLEDLRGMVRLRTLT---- 729

Query: 734  LQFCTTADLKNLE-----LHWDIKMDHEDALDDGDNDDEGVLEGLKPHSNIRKMIIKGYR 793
            ++      L+ L      L +  K++ +D        + G++       +++++ ++ Y 
Sbjct: 730  IELIEETSLETLAASIGGLKYLEKLEIDDLGSKMRTKEAGIVFDF---VHLKRLRLELYM 789

Query: 794  GMKLCDWFSSNFLGGLVSIELSHCEKLEH-LPQFDQFLYLKHLLLGYLPNIEYIDSGNSV 853
                 +    +F   L ++ L HC   E  +P  ++ L LK L LG+        SG  +
Sbjct: 790  PRLSKE---QHFPSHLTTLYLQHCRLEEDPMPILEKLLQLKELELGHKS-----FSGKKM 849

Query: 854  SSSTTFFPSLEKLRIESMPKLKGWWKGEISFPTTILHQLSELCIFYCPLLAS-----IPQ 913
              S+  FP L+KL I  + + + W   E S P      L  L IF C  L       +P 
Sbjct: 850  VCSSCGFPQLQKLSISGLKEWEDWKVEESSMPL-----LLTLNIFDCRKLKQLPDEHLPS 909

Query: 914  HPSLESLRICGVS---VQLFQMVIRMATDLSEHSSSSSTLSKLSFLEIGTIDLEFLP--- 973
            H +  SL+ CG+      L ++V      LSE        +   F ++  +DL  L    
Sbjct: 910  HLTAISLKKCGLEDPIPTLERLVHLKELSLSELCGRIMVCTGGGFPQLHKLDLSELDGLE 969

Query: 974  --VELFCNMTHLESLIIERCKSL--------QMSSPH--PVDEDNDVVWKKLSNLRTLRL 1033
              +    +M  L +L I RC  L        Q+ + H   V+E  + +  K  ++  L  
Sbjct: 970  EWIVEDGSMPRLHTLEIRRCLKLKKLPNGFPQLQNLHLTEVEEWEEGMIVKQGSMPLLHT 1029

Query: 1034 ESILKLEYFPKSLKYITSLETLKLSNCENLVSTEGIGELISLSHLEIDRCPNLPILSEDV 1093
              I      P    + + L T+ L      V  + +  L  L HL+     N+ +     
Sbjct: 1030 LYIWHCPKLPGEQHFPSHLTTVFLLGM--YVEEDPMRILEKLLHLK-----NVSLFQSFS 1089

Query: 1094 GDLISLSHLLIWNCPKLTSLS-------EGITRLTS---LSSLCLEDCPNLVSLPQEFLH 1127
            G  +  S       P+L  LS       E I    S   L +L +  CPNL  LP     
Sbjct: 1090 GKRMVCSG---GGFPQLQKLSIREIEWEEWIVEQGSMPLLHTLYIGVCPNLKELPD---- 1131

BLAST of Cucsa.128100 vs. TAIR10
Match: AT3G50950.2 (AT3G50950.2 HOPZ-ACTIVATED RESISTANCE 1)

HSP 1 Score: 263.1 bits (671), Expect = 8.0e-70
Identity = 211/766 (27.55%), Postives = 370/766 (48.30%), Query Frame = 1

Query: 34  ELDKLQNTLSAIKAVLLDAEEQQSKSHTVKDWIAKIKDVFYDIDDLIDEFSYETLRRQVL 93
           +L+ LQ+ L  +++ L DAE Q+  + T++  +A ++++ Y+ +D++ +           
Sbjct: 30  QLEDLQSELKYMQSFLKDAERQKRTNETLRTLVADLRELVYEAEDILVDCQLAD------ 89

Query: 94  TKDRTITKQVRIFFSKSN--QIAFGFKMGQTIKKVREKLDAIAA-IKAQLHLSVCAREVR 153
             D    +    + S+ +  ++   +K  + ++++ E++  I + ++        +   R
Sbjct: 90  GDDGNEQRSSNAWLSRLHPARVPLQYKKSKRLQEINERITKIKSQVEPYFEFITPSNVGR 149

Query: 154 DNEPRKVRETSSFIPEGEIIGRDEDRKSVMDFLLNTSNITKDNVEVVSIVGMGGLGKTAL 213
           DN     R +S      +++G + D++ + ++L  +++     + +++ VGMGGLGKT +
Sbjct: 150 DNGTD--RWSSPVYDHTQVVGLEGDKRKIKEWLFRSND---SQLLIMAFVGMGGLGKTTI 209

Query: 214 AQTVYNDEKINNRFKWKIWVCISQEFDIKVIVEKILESITKTKQESLQLDILQSMLQEKI 273
           AQ V+ND++I +RF+ +IWV +SQ F  + I+  IL ++         +  L   +Q+ +
Sbjct: 210 AQEVFNDKEIEHRFERRIWVSVSQTFTEEQIMRSILRNLGDASVGD-DIGTLLRKIQQYL 269

Query: 274 YGKKYLLVMDDVWNVDHEKWIGLKRFLMGGASGSKILVTTRNLQTA---QASDTVWFHHL 333
            GK+YL+VMDDVW+ +   W  + + L  G  GS ++VTTR+   A   QA D    H  
Sbjct: 270 LGKRYLIVMDDVWDKNLSWWDKIYQGLPRGQGGS-VIVTTRSESVAKRVQARDDKT-HRP 329

Query: 334 KELDKDNSWALFRKMAFLNKEEELENSNLVRIGKEIVAKLKGYPLSIRVVGRLLYFKN-T 393
           + L  DNSW LF  +AF   +   E   L  +GKEIV K KG PL+I+ VG LL  K+  
Sbjct: 330 ELLSPDNSWLLFCNVAFAANDGTCERPELEDVGKEIVTKCKGLPLTIKAVGGLLLCKDHV 389

Query: 394 EMDWSSFKDN---ELDSILQEDDQIQPILKISFNHLPPKLKQCFTYCALFPKDYEFKKNG 453
             +W    ++   EL     E D +   L++S++ LP  LK C    +L+P+D    K  
Sbjct: 390 YHEWRRIAEHFQDELRGNTSETDNVMSSLQLSYDELPSHLKSCILTLSLYPEDCVIPKQQ 449

Query: 454 LVKQWMAQGFIQAHNKKAIEDVGDDYFQELVGRSFFQDIRKNKWGDLKYCKMHDLLHDLA 513
           LV  W+ +GF+   N ++  + G+D F  L  R   + + K   G +  CK+HD++ DL 
Sbjct: 450 LVHGWIGEGFVMWRNGRSATESGEDCFSGLTNRCLIEVVDKTYSGTIITCKIHDMVRDLV 509

Query: 514 CSIGENECVVVSDDV--------GSIDKRTRHASFLLSKRLTREVVSKSSIEVTSLRTLD 573
             I + +     + +        G+ D++    +  L     R VV  S+ +   +  L+
Sbjct: 510 IDIAKKDSFSNPEGLNCRHLGISGNFDEKQIKVNHKL-----RGVV--STTKTGEVNKLN 569

Query: 574 IDSRASFRSFKKTCHMNLFQLRTLNLDRCCCHPP--KFVDK---LKHLRYLNLSGLN-VT 633
            D    F   K         LR L++ +     P  + +D+   L+HL  L+LS  + + 
Sbjct: 570 SDLAKKFTDCK--------YLRVLDISKSIFDAPLSEILDEIASLQHLACLSLSNTHPLI 629

Query: 634 FLPNSITTLYNLETLILRYCLWLRKLPKDINNLINLRHLDIYDCSSLTHMPKGLGGMTSL 693
             P S+  L+NL+ L   YC  L++L   I     L  LD+ +C SL   PKG+G +  L
Sbjct: 630 QFPRSMEDLHNLQILDASYCQNLKQLQPCIVLFKKLLVLDMTNCGSLECFPKGIGSLVKL 689

Query: 694 QTMSMFVLGK-NKGGDLSALNGLKSLR--GLLCIKGLQFCTTA--DLKNLELHWDIKMDH 753
           + +  F   + N G  LS +  L +LR  GL   +G Q        L NL     I ++ 
Sbjct: 690 EVLLGFKPARSNNGCKLSEVKNLTNLRKLGLSLTRGDQIEEEELDSLINLSKLMSISINC 749

Query: 754 EDALDDGDNDDEGVLEGLKPHSNIRKMIIKGYRGMKLCDWFSSNFL 771
            D+  D   D    ++ L P   + ++ ++ Y G     W S + L
Sbjct: 750 YDSYGD---DLITKIDALTPPHQLHELSLQFYPGKSSPSWLSPHKL 763

BLAST of Cucsa.128100 vs. TAIR10
Match: AT3G46730.1 (AT3G46730.1 NB-ARC domain-containing disease resistance protein)

HSP 1 Score: 256.5 bits (654), Expect = 7.5e-68
Identity = 188/647 (29.06%), Postives = 317/647 (49.00%), Query Frame = 1

Query: 9   VAASVITKLGSSALRELGSLWGVNDELDKLQNTLSAIKAVLLDAEEQQSKSHTVKDWIAK 68
           V   V+ K+G   + E+ +L GV D+L++L+  L+ I   L D E ++ +    K+W   
Sbjct: 5   VTGFVLNKIGGYLINEVLALMGVKDDLEELKTELTCIHGYLKDVEAREREDEVSKEWTKL 64

Query: 69  IKDVFYDIDDLIDEFSYETLRRQVLTKDRTITKQVRIFFSKSNQIAFGFKMGQTIKKVRE 128
           + D+ YDI+D++D +  +        ++R++ + +    +K  +    + + + I+ ++ 
Sbjct: 65  VLDIAYDIEDVLDTYFLKL-------EERSLRRGLLRLTNKIGKKRDAYNIVEDIRTLKR 124

Query: 129 KLDAIAAIKAQLHLSVCAREVRDN----EPRKVRETSSFIPEGEIIGRDEDRKSVMDFLL 188
           ++  I   +    +        +N      R++R       E  ++G ++D K ++  LL
Sbjct: 125 RILDITRKRETFGIGSFNEPRGENITNVRVRQLRRAPPVDQEELVVGLEDDVKILLVKLL 184

Query: 189 NTSNITKDNVEVVSIVGMGGLGKTALAQTVYNDEKINNRFKWKIWVCISQEFDIKVIVEK 248
           + +   KD   ++SI GMGGLGKTALA+ +YN   +  RF  + W  +SQE+  + I+ +
Sbjct: 185 SDNE--KDKSYIISIFGMGGLGKTALARKLYNSGDVKRRFDCRAWTYVSQEYKTRDILIR 244

Query: 249 ILESITKTKQESLQL-------DILQSMLQEKIYGKKYLLVMDDVWNVDHEKWIGLKRFL 308
           I+ S+     E ++        + L+  L   + GK Y++V+DDVW  D + W  LKR L
Sbjct: 245 IIRSLGIVSAEEMEKIKMFEEDEELEVYLYGLLEGKNYMVVVDDVW--DPDAWESLKRAL 304

Query: 309 MGGASGSKILVTTRNLQTAQASD-TVWFHHLKELDKDNSWALFRKMAFLNKEEELENSNL 368
                GSK+++TTR    A+  + TV+ H L+ L  + SW LF + AF N E+   + +L
Sbjct: 305 PCDHRGSKVIITTRIRAIAEGVEGTVYAHKLRFLTFEESWTLFERKAFSNIEK--VDEDL 364

Query: 369 VRIGKEIVAKLKGYPLSIRVVGRLLYFKNTE-------MDWSSFKDNELDSILQEDDQIQ 428
            R GKE+V K  G PL+I V+  LL  K T          W   KDN +         I 
Sbjct: 365 QRTGKEMVKKCGGLPLAIVVLSGLLSRKRTNEWHEVCASLWRRLKDNSI--------HIS 424

Query: 429 PILKISFNHLPPKLKQCFTYCALFPKDYEFKKNGLVKQWMAQGFIQAHNKKAIEDVGDDY 488
            +  +SF  +  +LK CF Y ++FP+DYE K   L+   +A+GFIQ   +  +EDV   Y
Sbjct: 425 TVFDLSFKEMRHELKLCFLYFSVFPEDYEIKVEKLIHLLVAEGFIQEDEEMMMEDVARCY 484

Query: 489 FQELVGRSFFQDIRKNKWGDLKYCKMHDLLHDLACSIGENECVVVSDDVGSIDKRTRHAS 548
             ELV RS  +  R  + G +  C++HDLL DLA    +    V      ++    +H+S
Sbjct: 485 IDELVDRSLVKAERIER-GKVMSCRIHDLLRDLAIKKAKELNFV------NVYNEKQHSS 544

Query: 549 FLLSKRLTREVVS-----KSSIEVTSLRTLDIDSRASFRSFKKTCHMNLFQLRTLNLD-- 608
            +  + +   +++        +       L I  R  F  +  T ++ L  LR LN++  
Sbjct: 545 DICRREVVHHLMNDYYLCDRRVNKRMRSFLFIGERRGF-GYVNTTNLKLKLLRVLNMEGL 604

Query: 609 -----RCCCHPPKFVDKLKHLRYLNLSGLNVTFLPNSITTLYNLETL 625
                      P  + +L HLRYL ++   V+ LP SI+ L  L+TL
Sbjct: 605 LFVSKNISNTLPDVIGELIHLRYLGIADTYVSILPASISNLRFLQTL 622

BLAST of Cucsa.128100 vs. NCBI nr
Match: gi|778728529|ref|XP_011659434.1| (PREDICTED: putative disease resistance protein RGA1 isoform X1 [Cucumis sativus])

HSP 1 Score: 2260.3 bits (5856), Expect = 0.0e+00
Identity = 1130/1142 (98.95%), Postives = 1130/1142 (98.95%), Query Frame = 1

Query: 1    MADSVLFNVAASVITKLGSSALRELGSLWGVNDELDKLQNTLSAIKAVLLDAEEQQSKSH 60
            MADSVLFNVAASVITKLGSSALRELGSLWGVNDELDKLQNTLSAIKAVLLDAEEQQSKSH
Sbjct: 1    MADSVLFNVAASVITKLGSSALRELGSLWGVNDELDKLQNTLSAIKAVLLDAEEQQSKSH 60

Query: 61   TVKDWIAKIKDVFYDIDDLIDEFSYETLRRQVLTKDRTITKQVRIFFSKSNQIAFGFKMG 120
            TVKDWIAKIKDVFYDIDDLIDEFSYETLRRQVLTKDRTITKQVRIFFSKSNQIAFGFKMG
Sbjct: 61   TVKDWIAKIKDVFYDIDDLIDEFSYETLRRQVLTKDRTITKQVRIFFSKSNQIAFGFKMG 120

Query: 121  QTIKKVREKLDAIAAIKAQLHLSVCAREVRDNEPRKVRETSSFIPEGEIIGRDEDRKSVM 180
            QTIKKVREKLDAIAAIKAQLHLSVCAREVRDNEPRKVRETSSFIPEGEIIGRDEDRKSVM
Sbjct: 121  QTIKKVREKLDAIAAIKAQLHLSVCAREVRDNEPRKVRETSSFIPEGEIIGRDEDRKSVM 180

Query: 181  DFLLNTSNITKDNVEVVSIVGMGGLGKTALAQTVYNDEKINNRFKWKIWVCISQEFDIKV 240
            DFLLNTSNITKDNVEVVSIVGMGGLGKTALAQTVYNDEKINNRFKWKIWVCISQEFDIKV
Sbjct: 181  DFLLNTSNITKDNVEVVSIVGMGGLGKTALAQTVYNDEKINNRFKWKIWVCISQEFDIKV 240

Query: 241  IVEKILESITKTKQESLQLDILQSMLQEKIYGKKYLLVMDDVWNVDHEKWIGLKRFLMGG 300
            IVEKILESITKTKQESLQLDILQSMLQEKIYGKKYLLVMDDVWNVDHEKWIGLKRFLMGG
Sbjct: 241  IVEKILESITKTKQESLQLDILQSMLQEKIYGKKYLLVMDDVWNVDHEKWIGLKRFLMGG 300

Query: 301  ASGSKILVTTRNLQTAQASDTVWFHHLKELDKDNSWALFRKMAFLNKEEELENSNLVRIG 360
            ASGSKILVTTRNLQTAQASDTVWFHHLKELDKDNSWALFRKMAFLNKEEELENSNLVRIG
Sbjct: 301  ASGSKILVTTRNLQTAQASDTVWFHHLKELDKDNSWALFRKMAFLNKEEELENSNLVRIG 360

Query: 361  KEIVAKLKGYPLSIRVVGRLLYFKNTEMDWSSFKDNELDSILQEDDQIQPILKISFNHLP 420
            KEIVAKLKGYPLSIRVVGRLLYFKNTEMDWSSFKDNELDSILQEDDQIQPILKISFNHLP
Sbjct: 361  KEIVAKLKGYPLSIRVVGRLLYFKNTEMDWSSFKDNELDSILQEDDQIQPILKISFNHLP 420

Query: 421  PKLKQCFTYCALFPKDYEFKKNGLVKQWMAQGFIQAHNKKAIEDVGDDYFQELVGRSFFQ 480
            PKLKQCFTYCALFPKDYEFKKNGLVKQWMAQGFIQAHNKKAIEDVGDDYFQELVGRSFFQ
Sbjct: 421  PKLKQCFTYCALFPKDYEFKKNGLVKQWMAQGFIQAHNKKAIEDVGDDYFQELVGRSFFQ 480

Query: 481  DIRKNKWGDLKYCKMHDLLHDLACSIGENECVVVSDDVGSIDKRTRHASFLLSKRLTREV 540
            DIRKNKWGDLKYCKMHDLLHDLACSIGENECVVVSDDVGSIDKRTRHASFLLSKRLTREV
Sbjct: 481  DIRKNKWGDLKYCKMHDLLHDLACSIGENECVVVSDDVGSIDKRTRHASFLLSKRLTREV 540

Query: 541  VSKSSIEVTSLRTLDIDSRASFRSFKKTCHMNLFQLRTLNLDRCCCHPPKFVDKLKHLRY 600
            VSKSSIEVTSLRTLDIDSRASFRSFKKTCHMNLFQLRTLNLDRCCCHPPKFVDKLKHLRY
Sbjct: 541  VSKSSIEVTSLRTLDIDSRASFRSFKKTCHMNLFQLRTLNLDRCCCHPPKFVDKLKHLRY 600

Query: 601  LNLSGLNVTFLPNSITTLYNLETLILRYCLWLRKLPKDINNLINLRHLDIYDCSSLTHMP 660
            LNLSGLNVTFLPNSITTLYNLETLILRYCLWLRKLPKDINNLINLRHLDIYDCSSLTHMP
Sbjct: 601  LNLSGLNVTFLPNSITTLYNLETLILRYCLWLRKLPKDINNLINLRHLDIYDCSSLTHMP 660

Query: 661  KGLGGMTSLQTMSMFVLGKNKGGDLSALNGLKSLRGLLCIKGLQFCTTADLKN------- 720
            KGLGGMTSLQTMSMFVLGKNKGGDLSALNGLKSLRGLLCIKGLQFCTTADLKN       
Sbjct: 661  KGLGGMTSLQTMSMFVLGKNKGGDLSALNGLKSLRGLLCIKGLQFCTTADLKNVSYLKEM 720

Query: 721  -----LELHWDIKMDHEDALDDGDNDDEGVLEGLKPHSNIRKMIIKGYRGMKLCDWFSSN 780
                 LELHWDIKMDHEDALDDGDNDDEGVLEGLKPHSNIRKMIIKGYRGMKLCDWFSSN
Sbjct: 721  YGIQKLELHWDIKMDHEDALDDGDNDDEGVLEGLKPHSNIRKMIIKGYRGMKLCDWFSSN 780

Query: 781  FLGGLVSIELSHCEKLEHLPQFDQFLYLKHLLLGYLPNIEYIDSGNSVSSSTTFFPSLEK 840
            FLGGLVSIELSHCEKLEHLPQFDQFLYLKHLLLGYLPNIEYIDSGNSVSSSTTFFPSLEK
Sbjct: 781  FLGGLVSIELSHCEKLEHLPQFDQFLYLKHLLLGYLPNIEYIDSGNSVSSSTTFFPSLEK 840

Query: 841  LRIESMPKLKGWWKGEISFPTTILHQLSELCIFYCPLLASIPQHPSLESLRICGVSVQLF 900
            LRIESMPKLKGWWKGEISFPTTILHQLSELCIFYCPLLASIPQHPSLESLRICGVSVQLF
Sbjct: 841  LRIESMPKLKGWWKGEISFPTTILHQLSELCIFYCPLLASIPQHPSLESLRICGVSVQLF 900

Query: 901  QMVIRMATDLSEHSSSSSTLSKLSFLEIGTIDLEFLPVELFCNMTHLESLIIERCKSLQM 960
            QMVIRMATDLSEHSSSSSTLSKLSFLEIGTIDLEFLPVELFCNMTHLESLIIERCKSLQM
Sbjct: 901  QMVIRMATDLSEHSSSSSTLSKLSFLEIGTIDLEFLPVELFCNMTHLESLIIERCKSLQM 960

Query: 961  SSPHPVDEDNDVVWKKLSNLRTLRLESILKLEYFPKSLKYITSLETLKLSNCENLVSTEG 1020
            SSPHPVDEDNDVVWKKLSNLRTLRLESILKLEYFPKSLKYITSLETLKLSNCENLVSTEG
Sbjct: 961  SSPHPVDEDNDVVWKKLSNLRTLRLESILKLEYFPKSLKYITSLETLKLSNCENLVSTEG 1020

Query: 1021 IGELISLSHLEIDRCPNLPILSEDVGDLISLSHLLIWNCPKLTSLSEGITRLTSLSSLCL 1080
            IGELISLSHLEIDRCPNLPILSEDVGDLISLSHLLIWNCPKLTSLSEGITRLTSLSSLCL
Sbjct: 1021 IGELISLSHLEIDRCPNLPILSEDVGDLISLSHLLIWNCPKLTSLSEGITRLTSLSSLCL 1080

Query: 1081 EDCPNLVSLPQEFLHHHSSLPGGRFLRILNCPKLQIQDKKQKEEEEEDQEDWNELIHVLT 1131
            EDCPNLVSLPQEFLHHHSSLPGGRFLRILNCPKLQIQDKKQKEEEEEDQEDWNELIHVLT
Sbjct: 1081 EDCPNLVSLPQEFLHHHSSLPGGRFLRILNCPKLQIQDKKQKEEEEEDQEDWNELIHVLT 1140

BLAST of Cucsa.128100 vs. NCBI nr
Match: gi|778728533|ref|XP_011659435.1| (PREDICTED: putative disease resistance protein RGA1 isoform X2 [Cucumis sativus])

HSP 1 Score: 2260.3 bits (5856), Expect = 0.0e+00
Identity = 1130/1142 (98.95%), Postives = 1130/1142 (98.95%), Query Frame = 1

Query: 1    MADSVLFNVAASVITKLGSSALRELGSLWGVNDELDKLQNTLSAIKAVLLDAEEQQSKSH 60
            MADSVLFNVAASVITKLGSSALRELGSLWGVNDELDKLQNTLSAIKAVLLDAEEQQSKSH
Sbjct: 1    MADSVLFNVAASVITKLGSSALRELGSLWGVNDELDKLQNTLSAIKAVLLDAEEQQSKSH 60

Query: 61   TVKDWIAKIKDVFYDIDDLIDEFSYETLRRQVLTKDRTITKQVRIFFSKSNQIAFGFKMG 120
            TVKDWIAKIKDVFYDIDDLIDEFSYETLRRQVLTKDRTITKQVRIFFSKSNQIAFGFKMG
Sbjct: 61   TVKDWIAKIKDVFYDIDDLIDEFSYETLRRQVLTKDRTITKQVRIFFSKSNQIAFGFKMG 120

Query: 121  QTIKKVREKLDAIAAIKAQLHLSVCAREVRDNEPRKVRETSSFIPEGEIIGRDEDRKSVM 180
            QTIKKVREKLDAIAAIKAQLHLSVCAREVRDNEPRKVRETSSFIPEGEIIGRDEDRKSVM
Sbjct: 121  QTIKKVREKLDAIAAIKAQLHLSVCAREVRDNEPRKVRETSSFIPEGEIIGRDEDRKSVM 180

Query: 181  DFLLNTSNITKDNVEVVSIVGMGGLGKTALAQTVYNDEKINNRFKWKIWVCISQEFDIKV 240
            DFLLNTSNITKDNVEVVSIVGMGGLGKTALAQTVYNDEKINNRFKWKIWVCISQEFDIKV
Sbjct: 181  DFLLNTSNITKDNVEVVSIVGMGGLGKTALAQTVYNDEKINNRFKWKIWVCISQEFDIKV 240

Query: 241  IVEKILESITKTKQESLQLDILQSMLQEKIYGKKYLLVMDDVWNVDHEKWIGLKRFLMGG 300
            IVEKILESITKTKQESLQLDILQSMLQEKIYGKKYLLVMDDVWNVDHEKWIGLKRFLMGG
Sbjct: 241  IVEKILESITKTKQESLQLDILQSMLQEKIYGKKYLLVMDDVWNVDHEKWIGLKRFLMGG 300

Query: 301  ASGSKILVTTRNLQTAQASDTVWFHHLKELDKDNSWALFRKMAFLNKEEELENSNLVRIG 360
            ASGSKILVTTRNLQTAQASDTVWFHHLKELDKDNSWALFRKMAFLNKEEELENSNLVRIG
Sbjct: 301  ASGSKILVTTRNLQTAQASDTVWFHHLKELDKDNSWALFRKMAFLNKEEELENSNLVRIG 360

Query: 361  KEIVAKLKGYPLSIRVVGRLLYFKNTEMDWSSFKDNELDSILQEDDQIQPILKISFNHLP 420
            KEIVAKLKGYPLSIRVVGRLLYFKNTEMDWSSFKDNELDSILQEDDQIQPILKISFNHLP
Sbjct: 361  KEIVAKLKGYPLSIRVVGRLLYFKNTEMDWSSFKDNELDSILQEDDQIQPILKISFNHLP 420

Query: 421  PKLKQCFTYCALFPKDYEFKKNGLVKQWMAQGFIQAHNKKAIEDVGDDYFQELVGRSFFQ 480
            PKLKQCFTYCALFPKDYEFKKNGLVKQWMAQGFIQAHNKKAIEDVGDDYFQELVGRSFFQ
Sbjct: 421  PKLKQCFTYCALFPKDYEFKKNGLVKQWMAQGFIQAHNKKAIEDVGDDYFQELVGRSFFQ 480

Query: 481  DIRKNKWGDLKYCKMHDLLHDLACSIGENECVVVSDDVGSIDKRTRHASFLLSKRLTREV 540
            DIRKNKWGDLKYCKMHDLLHDLACSIGENECVVVSDDVGSIDKRTRHASFLLSKRLTREV
Sbjct: 481  DIRKNKWGDLKYCKMHDLLHDLACSIGENECVVVSDDVGSIDKRTRHASFLLSKRLTREV 540

Query: 541  VSKSSIEVTSLRTLDIDSRASFRSFKKTCHMNLFQLRTLNLDRCCCHPPKFVDKLKHLRY 600
            VSKSSIEVTSLRTLDIDSRASFRSFKKTCHMNLFQLRTLNLDRCCCHPPKFVDKLKHLRY
Sbjct: 541  VSKSSIEVTSLRTLDIDSRASFRSFKKTCHMNLFQLRTLNLDRCCCHPPKFVDKLKHLRY 600

Query: 601  LNLSGLNVTFLPNSITTLYNLETLILRYCLWLRKLPKDINNLINLRHLDIYDCSSLTHMP 660
            LNLSGLNVTFLPNSITTLYNLETLILRYCLWLRKLPKDINNLINLRHLDIYDCSSLTHMP
Sbjct: 601  LNLSGLNVTFLPNSITTLYNLETLILRYCLWLRKLPKDINNLINLRHLDIYDCSSLTHMP 660

Query: 661  KGLGGMTSLQTMSMFVLGKNKGGDLSALNGLKSLRGLLCIKGLQFCTTADLKN------- 720
            KGLGGMTSLQTMSMFVLGKNKGGDLSALNGLKSLRGLLCIKGLQFCTTADLKN       
Sbjct: 661  KGLGGMTSLQTMSMFVLGKNKGGDLSALNGLKSLRGLLCIKGLQFCTTADLKNVSYLKEM 720

Query: 721  -----LELHWDIKMDHEDALDDGDNDDEGVLEGLKPHSNIRKMIIKGYRGMKLCDWFSSN 780
                 LELHWDIKMDHEDALDDGDNDDEGVLEGLKPHSNIRKMIIKGYRGMKLCDWFSSN
Sbjct: 721  YGIQKLELHWDIKMDHEDALDDGDNDDEGVLEGLKPHSNIRKMIIKGYRGMKLCDWFSSN 780

Query: 781  FLGGLVSIELSHCEKLEHLPQFDQFLYLKHLLLGYLPNIEYIDSGNSVSSSTTFFPSLEK 840
            FLGGLVSIELSHCEKLEHLPQFDQFLYLKHLLLGYLPNIEYIDSGNSVSSSTTFFPSLEK
Sbjct: 781  FLGGLVSIELSHCEKLEHLPQFDQFLYLKHLLLGYLPNIEYIDSGNSVSSSTTFFPSLEK 840

Query: 841  LRIESMPKLKGWWKGEISFPTTILHQLSELCIFYCPLLASIPQHPSLESLRICGVSVQLF 900
            LRIESMPKLKGWWKGEISFPTTILHQLSELCIFYCPLLASIPQHPSLESLRICGVSVQLF
Sbjct: 841  LRIESMPKLKGWWKGEISFPTTILHQLSELCIFYCPLLASIPQHPSLESLRICGVSVQLF 900

Query: 901  QMVIRMATDLSEHSSSSSTLSKLSFLEIGTIDLEFLPVELFCNMTHLESLIIERCKSLQM 960
            QMVIRMATDLSEHSSSSSTLSKLSFLEIGTIDLEFLPVELFCNMTHLESLIIERCKSLQM
Sbjct: 901  QMVIRMATDLSEHSSSSSTLSKLSFLEIGTIDLEFLPVELFCNMTHLESLIIERCKSLQM 960

Query: 961  SSPHPVDEDNDVVWKKLSNLRTLRLESILKLEYFPKSLKYITSLETLKLSNCENLVSTEG 1020
            SSPHPVDEDNDVVWKKLSNLRTLRLESILKLEYFPKSLKYITSLETLKLSNCENLVSTEG
Sbjct: 961  SSPHPVDEDNDVVWKKLSNLRTLRLESILKLEYFPKSLKYITSLETLKLSNCENLVSTEG 1020

Query: 1021 IGELISLSHLEIDRCPNLPILSEDVGDLISLSHLLIWNCPKLTSLSEGITRLTSLSSLCL 1080
            IGELISLSHLEIDRCPNLPILSEDVGDLISLSHLLIWNCPKLTSLSEGITRLTSLSSLCL
Sbjct: 1021 IGELISLSHLEIDRCPNLPILSEDVGDLISLSHLLIWNCPKLTSLSEGITRLTSLSSLCL 1080

Query: 1081 EDCPNLVSLPQEFLHHHSSLPGGRFLRILNCPKLQIQDKKQKEEEEEDQEDWNELIHVLT 1131
            EDCPNLVSLPQEFLHHHSSLPGGRFLRILNCPKLQIQDKKQKEEEEEDQEDWNELIHVLT
Sbjct: 1081 EDCPNLVSLPQEFLHHHSSLPGGRFLRILNCPKLQIQDKKQKEEEEEDQEDWNELIHVLT 1140

BLAST of Cucsa.128100 vs. NCBI nr
Match: gi|700189869|gb|KGN45102.1| (hypothetical protein Csa_7G425940 [Cucumis sativus])

HSP 1 Score: 1580.1 bits (4090), Expect = 0.0e+00
Identity = 781/793 (98.49%), Postives = 781/793 (98.49%), Query Frame = 1

Query: 350  ELENSNLVRIGKEIVAKLKGYPLSIRVVGRLLYFKNTEMDWSSFKDNELDSILQEDDQIQ 409
            ELENSNLVRIGKEIVAKLKGYPLSIRVVGRLLYFKNTEMDWSSFKDNELDSILQEDDQIQ
Sbjct: 171  ELENSNLVRIGKEIVAKLKGYPLSIRVVGRLLYFKNTEMDWSSFKDNELDSILQEDDQIQ 230

Query: 410  PILKISFNHLPPKLKQCFTYCALFPKDYEFKKNGLVKQWMAQGFIQAHNKKAIEDVGDDY 469
            PILKISFNHLPPKLKQCFTYCALFPKDYEFKKNGLVKQWMAQGFIQAHNKKAIEDVGDDY
Sbjct: 231  PILKISFNHLPPKLKQCFTYCALFPKDYEFKKNGLVKQWMAQGFIQAHNKKAIEDVGDDY 290

Query: 470  FQELVGRSFFQDIRKNKWGDLKYCKMHDLLHDLACSIGENECVVVSDDVGSIDKRTRHAS 529
            FQELVGRSFFQDIRKNKWGDLKYCKMHDLLHDLACSIGENECVVVSDDVGSIDKRTRHAS
Sbjct: 291  FQELVGRSFFQDIRKNKWGDLKYCKMHDLLHDLACSIGENECVVVSDDVGSIDKRTRHAS 350

Query: 530  FLLSKRLTREVVSKSSIEVTSLRTLDIDSRASFRSFKKTCHMNLFQLRTLNLDRCCCHPP 589
            FLLSKRLTREVVSKSSIEVTSLRTLDIDSRASFRSFKKTCHMNLFQLRTLNLDRCCCHPP
Sbjct: 351  FLLSKRLTREVVSKSSIEVTSLRTLDIDSRASFRSFKKTCHMNLFQLRTLNLDRCCCHPP 410

Query: 590  KFVDKLKHLRYLNLSGLNVTFLPNSITTLYNLETLILRYCLWLRKLPKDINNLINLRHLD 649
            KFVDKLKHLRYLNLSGLNVTFLPNSITTLYNLETLILRYCLWLRKLPKDINNLINLRHLD
Sbjct: 411  KFVDKLKHLRYLNLSGLNVTFLPNSITTLYNLETLILRYCLWLRKLPKDINNLINLRHLD 470

Query: 650  IYDCSSLTHMPKGLGGMTSLQTMSMFVLGKNKGGDLSALNGLKSLRGLLCIKGLQFCTTA 709
            IYDCSSLTHMPKGLGGMTSLQTMSMFVLGKNKGGDLSALNGLKSLRGLLCIKGLQFCTTA
Sbjct: 471  IYDCSSLTHMPKGLGGMTSLQTMSMFVLGKNKGGDLSALNGLKSLRGLLCIKGLQFCTTA 530

Query: 710  DLKN------------LELHWDIKMDHEDALDDGDNDDEGVLEGLKPHSNIRKMIIKGYR 769
            DLKN            LELHWDIKMDHEDALDDGDNDDEGVLEGLKPHSNIRKMIIKGYR
Sbjct: 531  DLKNVSYLKEMYGIQKLELHWDIKMDHEDALDDGDNDDEGVLEGLKPHSNIRKMIIKGYR 590

Query: 770  GMKLCDWFSSNFLGGLVSIELSHCEKLEHLPQFDQFLYLKHLLLGYLPNIEYIDSGNSVS 829
            GMKLCDWFSSNFLGGLVSIELSHCEKLEHLPQFDQFLYLKHLLLGYLPNIEYIDSGNSVS
Sbjct: 591  GMKLCDWFSSNFLGGLVSIELSHCEKLEHLPQFDQFLYLKHLLLGYLPNIEYIDSGNSVS 650

Query: 830  SSTTFFPSLEKLRIESMPKLKGWWKGEISFPTTILHQLSELCIFYCPLLASIPQHPSLES 889
            SSTTFFPSLEKLRIESMPKLKGWWKGEISFPTTILHQLSELCIFYCPLLASIPQHPSLES
Sbjct: 651  SSTTFFPSLEKLRIESMPKLKGWWKGEISFPTTILHQLSELCIFYCPLLASIPQHPSLES 710

Query: 890  LRICGVSVQLFQMVIRMATDLSEHSSSSSTLSKLSFLEIGTIDLEFLPVELFCNMTHLES 949
            LRICGVSVQLFQMVIRMATDLSEHSSSSSTLSKLSFLEIGTIDLEFLPVELFCNMTHLES
Sbjct: 711  LRICGVSVQLFQMVIRMATDLSEHSSSSSTLSKLSFLEIGTIDLEFLPVELFCNMTHLES 770

Query: 950  LIIERCKSLQMSSPHPVDEDNDVVWKKLSNLRTLRLESILKLEYFPKSLKYITSLETLKL 1009
            LIIERCKSLQMSSPHPVDEDNDVVWKKLSNLRTLRLESILKLEYFPKSLKYITSLETLKL
Sbjct: 771  LIIERCKSLQMSSPHPVDEDNDVVWKKLSNLRTLRLESILKLEYFPKSLKYITSLETLKL 830

Query: 1010 SNCENLVSTEGIGELISLSHLEIDRCPNLPILSEDVGDLISLSHLLIWNCPKLTSLSEGI 1069
            SNCENLVSTEGIGELISLSHLEIDRCPNLPILSEDVGDLISLSHLLIWNCPKLTSLSEGI
Sbjct: 831  SNCENLVSTEGIGELISLSHLEIDRCPNLPILSEDVGDLISLSHLLIWNCPKLTSLSEGI 890

Query: 1070 TRLTSLSSLCLEDCPNLVSLPQEFLHHHSSLPGGRFLRILNCPKLQIQDKKQKEEEEEDQ 1129
            TRLTSLSSLCLEDCPNLVSLPQEFLHHHSSLPGGRFLRILNCPKLQIQDKKQKEEEEEDQ
Sbjct: 891  TRLTSLSSLCLEDCPNLVSLPQEFLHHHSSLPGGRFLRILNCPKLQIQDKKQKEEEEEDQ 950

Query: 1130 EDWNELIHVLTGC 1131
            EDWNELIHVLTGC
Sbjct: 951  EDWNELIHVLTGC 963

BLAST of Cucsa.128100 vs. NCBI nr
Match: gi|700189869|gb|KGN45102.1| (hypothetical protein Csa_7G425940 [Cucumis sativus])

HSP 1 Score: 328.6 bits (841), Expect = 4.4e-86
Identity = 175/198 (88.38%), Postives = 179/198 (90.40%), Query Frame = 1

Query: 1   MADSVLFNVAASVITKLGSSALRELGSLWGVNDELDKLQNTLSAIKAVLLDAEEQQSKSH 60
           MADSVLFNVAASVITKLGSSALRELGSLWGVNDELDKLQNTLSAIKAVLLDAEEQQSKSH
Sbjct: 1   MADSVLFNVAASVITKLGSSALRELGSLWGVNDELDKLQNTLSAIKAVLLDAEEQQSKSH 60

Query: 61  TVKDWIAKIKDVFYDIDDLIDEFSYETLRRQVLTKDRTITKQVRIFFSKSNQIAFGFKMG 120
           TVKDWIAKIKDVFYDIDDLIDEFSYETLRRQVLTKDRTITKQVRIFFSKSNQIAFGFKMG
Sbjct: 61  TVKDWIAKIKDVFYDIDDLIDEFSYETLRRQVLTKDRTITKQVRIFFSKSNQIAFGFKMG 120

Query: 121 QTIKKVREKLDAIAAIKAQLHLSVCAREVRDNEPRKVRETSSFIPEGEIIGRDEDRKSVM 180
           QTIKKVREKLDAIAAIKAQLHLSVCAREVRDNEPRKVRETSSFIPEGEII          
Sbjct: 121 QTIKKVREKLDAIAAIKAQLHLSVCAREVRDNEPRKVRETSSFIPEGEIIE--------- 180

Query: 181 DFLLNTSNITKDNVEVVS 199
              L  SN+ +   E+V+
Sbjct: 181 ---LENSNLVRIGKEIVA 186


HSP 2 Score: 1408.3 bits (3644), Expect = 0.0e+00
Identity = 755/1126 (67.05%), Postives = 871/1126 (77.35%), Query Frame = 1

Query: 1    MADSVLFNVAASVITKLGSSALRELGSLWGVNDELDKLQNTLSAIKAVLLDAEEQQSKSH 60
            MADS+LFNVAA+VITKLGSSALRELGSLWGVNDEL KLQN LSAIKAVLLDAEEQQS SH
Sbjct: 27   MADSILFNVAANVITKLGSSALRELGSLWGVNDELGKLQNILSAIKAVLLDAEEQQSVSH 86

Query: 61   TVKDWIAKIKDVFYDIDDLIDEFSYETLRRQVLTKDRTITKQVRIFFSKSNQIAFGFKMG 120
             VKDWI+K++DVFYD+DDLIDEFSYETLRRQVLTKDRTITKQV IFFSKSNQ++FG KM 
Sbjct: 87   AVKDWISKLRDVFYDVDDLIDEFSYETLRRQVLTKDRTITKQVCIFFSKSNQVSFGHKMS 146

Query: 121  QTIKKVREKLDAIAAIKAQLHLSVCAREVRDNEPRKVRETSSFIPEGEIIGRDEDRKSVM 180
            Q IK+VREKLDAIA  K QLHLSV  RE RD+E RK+RET SFIP+GE+IGRD+D+K+++
Sbjct: 147  QKIKQVREKLDAIANDKTQLHLSVRMRETRDDELRKMRETCSFIPKGEVIGRDDDKKAII 206

Query: 181  DFLLNTSNITKDNVEVVSIVGMGGLGKTALAQTVYNDEKINNRFKWKIWVCISQEFDIKV 240
            DFLL+T N  +DNVEVVSIVGMGGLGKTA+AQ+VYNDEKIN  FK K+WVCISQEFDIKV
Sbjct: 207  DFLLDT-NTMEDNVEVVSIVGMGGLGKTAVAQSVYNDEKINEHFKLKLWVCISQEFDIKV 266

Query: 241  IVEKILESITKTKQESLQLDILQSMLQEKIYGKKYLLVMDDVWNVDHEKWIGLKRFLMGG 300
            IVEKI+E I K K +SLQLDILQSMLQEKI GKKYLLVMDDVWN  HE W+ LKRFLMGG
Sbjct: 267  IVEKIIEFIAKKKPDSLQLDILQSMLQEKIDGKKYLLVMDDVWNESHETWVSLKRFLMGG 326

Query: 301  ASGSKILVTTRNLQTAQASDTVWFHHLKELDKDNSWALFRKMAFLNKEEELENSNLVRIG 360
            A GS+IL+TTRNLQ AQASDTV FHHLKELD ++SWALFRKMAFLN+EEE+ENSN VRIG
Sbjct: 327  AKGSRILITTRNLQVAQASDTVQFHHLKELDNESSWALFRKMAFLNEEEEIENSNKVRIG 386

Query: 361  KEIVAKLKGYPLSIRVVGRLLYFKNTEMDWSSFKDNELDSILQEDDQIQPILKISFNHLP 420
            KEI+AKLKG PL+IR+VGRLLYFKNTEMDW SFKDN+L +ILQ+++QIQPILKISFNHLP
Sbjct: 387  KEIIAKLKGSPLTIRIVGRLLYFKNTEMDWLSFKDNDLGTILQQENQIQPILKISFNHLP 446

Query: 421  PKLKQCFTYCALFPKDYEFKKNGLVKQWMAQGFIQAHNKKAIEDVGDDYFQELVGRSFFQ 480
              LK CFTYCALFPKDYEF+K+GLVKQWMAQGFIQ+H+ K IEDVGDDYF+EL+GRSFF 
Sbjct: 447  SNLKHCFTYCALFPKDYEFQKDGLVKQWMAQGFIQSHSNKEIEDVGDDYFKELLGRSFFH 506

Query: 481  DIRKNKWGDLKYCKMHDLLHDLACSIGENECVVVSDDVGSIDKRTRHASFLLS-KRLTRE 540
            +++ NKWGD+K CKMHDL+HDLAC I ENECV  SD   SIDKRTRH SF  +  R + E
Sbjct: 507  NVKVNKWGDVKECKMHDLIHDLACWIVENECVDASDKTKSIDKRTRHVSFPSNYSRKSWE 566

Query: 541  VVSKSSIEVTSLRTLDIDSRASFRSFKKTCHMNLFQLRTLNLDRCCCHP-PKFVDKLKHL 600
            + +KS  EV +LRTL                 N  +LR+LNL        PKF+ +L+HL
Sbjct: 567  LEAKSLTEVKNLRTL--------HGPPFLLSENHLRLRSLNLGYSKFQKIPKFISQLRHL 626

Query: 601  RYLNLSGLNVTFLPNSITTLYNLETLILRYCLWLRKLPKDINNLINLRHLDIYDCSSLTH 660
            RYL++S  ++ FLP  IT LYNLETLILR+C  LR+LP DINNLINL+HLD++ C  LTH
Sbjct: 627  RYLDISDHDMKFLPKFITKLYNLETLILRHCSDLRELPTDINNLINLKHLDVHGCYRLTH 686

Query: 661  MPKGLGGMTSLQTMSMFVLGKNKGGDLSALNGLKSLRGLLCIKGLQFCTTADLKN----- 720
            MPKGLGG+TSLQTM++FVLGK+KG DLS LN L  LRG L IKGL+ CTT DLKN     
Sbjct: 687  MPKGLGGLTSLQTMNLFVLGKDKGCDLSELNELARLRGSLLIKGLELCTTTDLKNAKYME 746

Query: 721  -------LELHWDIKMDHEDALDDGDNDDEGVLEGLKPHSNIRKMIIKGYRGMKLCDWFS 780
                   L+L W+  +   +     +NDDE VL+ LKPHSN+ KM I+GYRG+KLC+W S
Sbjct: 747  EKFGIQKLKLRWNRDLYDAETDYASENDDERVLDCLKPHSNVHKMQIRGYRGVKLCNWLS 806

Query: 781  SNFLGGLVSIELSHCEKLEHLPQFDQFLYLKHLLLGYLPNIEYIDSGNSVSSSTTFFPSL 840
             ++LGGLV+IEL  CEKL+HLPQFDQF +LKHLLL  LP+IEYID+ NS+SSS TFFPSL
Sbjct: 807  FDYLGGLVNIELQSCEKLQHLPQFDQFPFLKHLLLENLPSIEYIDNNNSLSSS-TFFPSL 866

Query: 841  EKLRIESMPKLKGWWKGEIS---------FPTTILHQLSELCIFYCPLLASIPQHPSLES 900
            EKL I +MP LKGWWKGE           FP TILH LS L I  CP LASIPQHP L S
Sbjct: 867  EKLTIMTMPNLKGWWKGETPPESARYSALFP-TILHHLSRLDISNCPQLASIPQHPPLRS 926

Query: 901  LRICGVSVQLFQMVIRMATDLSEHSSSSSTLSKLSFLEIGTIDLEFLPVELFCNMTHLES 960
            L +  VSVQLF MVI+MAT  +  + SSS LSKLS L I  IDLEFLP ELF + T LE 
Sbjct: 927  LALNDVSVQLFDMVIKMAT--TPAADSSSALSKLSILHIQNIDLEFLPEELFGSTTDLEI 986

Query: 961  LIIERCKSLQMSSPHPVDEDND-VVWKKLSNLRTLRLESILKLEYFPKSLKYITSLETLK 1020
              +  CK+LQMSS H VDEDND V+ KKL NL +L +  + +LEY  K LKY+T+LE L 
Sbjct: 987  FTVVNCKNLQMSSSHLVDEDNDGVLGKKLGNLHSLGIFDMPQLEYLWKELKYMTTLERLD 1046

Query: 1021 LSNCENLVSTEGIGELISLSHLEIDRCPNLPILSEDVGDLISLSHLLIWNCPKLTSLSEG 1080
            L NC N+VS EGI  L SLS L I  C NL  L E +  L SLS+L I  CP LTSL  G
Sbjct: 1047 LYNCPNIVSLEGISHLTSLSSLRICNCSNLTSLPEGISHLTSLSYLTIVCCPNLTSLPAG 1106

Query: 1081 ITRLTSLSSLCLEDCPNLVSLPQEFLHHHSSLPGGRFLRILNCPKL 1103
            I  LTSLS+L ++ C NL SLP E + H +SL       I  CP L
Sbjct: 1107 IGHLTSLSTLLIKYCVNLTSLP-EGVSHLTSLSS---FTIEECPCL 1135

BLAST of Cucsa.128100 vs. NCBI nr
Match: gi|778728520|ref|XP_011659433.1| (PREDICTED: putative disease resistance protein RGA4 [Cucumis sativus])

HSP 1 Score: 103.2 bits (256), Expect = 3.0e-18
Identity = 80/204 (39.22%), Postives = 102/204 (50.00%), Query Frame = 1

Query: 906  STLSKLSFLEIGTIDLEFLPVELFCNMTHLESLIIERCKSLQMSSPHPVDEDNDVVWKKL 965
            +TL +L       I    + +E   ++T L SL I  C +L  S P  +          L
Sbjct: 1027 TTLERLDLYNCPNI----VSLEGISHLTSLSSLRICNCSNLT-SLPEGISH--------L 1086

Query: 966  SNLRTLRLESILKLEYFPKSLKYITSLETLKLSNCENLVST-EGIGELISLSHLEIDRCP 1025
            ++L  L +     L   P  + ++TSL TL +  C NL S  EG+  L SLS   I+ CP
Sbjct: 1087 TSLSYLTIVCCPNLTSLPAGIGHLTSLSTLLIKYCVNLTSLPEGVSHLTSLSSFTIEECP 1146

Query: 1026 NLPILSEDVGDLISLSHLLIWNCPKLTSLSEGITRLTSLSSLCLEDCPNLVSLPQEFLHH 1085
             L  L E V  L SLS L+I  C  LTSL EGI  LTSLS   +E+C NL SLP+  L H
Sbjct: 1147 CLTSLPEGVSHLTSLSTLIIRRCVNLTSLPEGIGHLTSLSIFTIEECLNLTSLPEGLL-H 1206

Query: 1086 HSSLPGGRFLRILNCPKLQIQDKK 1109
             SSL G   L +  CPKL    KK
Sbjct: 1207 LSSLRGS--LTVSKCPKLSKTWKK 1214


HSP 2 Score: 50.4 bits (119), Expect = 2.3e-02
Identity = 34/103 (33.01%), Postives = 57/103 (55.34%), Query Frame = 1

Query: 572  NLFQLRTLNLDRCCCHPPKFVDKLKHLRYLNLSGLN----VTFLPNSITTLYNLETLILR 631
            +L  L TL L + C +     + + HL  L+   +     +T LP  ++ L +L TLI+R
Sbjct: 1096 HLTSLSTL-LIKYCVNLTSLPEGVSHLTSLSSFTIEECPCLTSLPEGVSHLTSLSTLIIR 1155

Query: 632  YCLWLRKLPKDINNLINLRHLDIYDCSSLTHMPKGLGGMTSLQ 671
             C+ L  LP+ I +L +L    I +C +LT +P+GL  ++SL+
Sbjct: 1156 RCVNLTSLPEGIGHLTSLSIFTIEECLNLTSLPEGLLHLSSLR 1197


HSP 3 Score: 1202.6 bits (3110), Expect = 0.0e+00
Identity = 648/1087 (59.61%), Postives = 802/1087 (73.78%), Query Frame = 1

Query: 1    MADSVLFNVAASVITKLGSSALRELGSLW-GVNDELDKLQNTLSAIKAVLLDAEEQQSKS 60
            MA+S+LF +AA++ TKLGS  L ELG LW G ++ELDKL++TLSAI+AVLLDAEE+Q KS
Sbjct: 1    MAESILFTLAANIATKLGSFPLHELGLLWTGFHEELDKLKDTLSAIQAVLLDAEEKQYKS 60

Query: 61   HTVKDWIAKIKDVFYDIDDLIDEFSYETLRRQVLTKDRTITKQVRIFFSKSNQIAFGFKM 120
            + VK+W++++KD FYDIDDL+DEFSYE+++RQV+ K RT  KQVRIFFS+SNQIAF  KM
Sbjct: 61   YAVKEWVSRLKDAFYDIDDLMDEFSYESIKRQVMIKHRTNNKQVRIFFSESNQIAFRLKM 120

Query: 121  GQTIKKVREKLDAIAAIKAQLHLSVCAREVRDNEPRKVRETSSFIPEGEIIGRDEDRKSV 180
            G  IK+VREKLD +A  KAQ +LS   RE+R++E  K  ETSSFI EGE+IGRD+D+K +
Sbjct: 121  GYKIKRVREKLDIVAIDKAQFNLSEYTREIRNDETTKRPETSSFILEGEVIGRDDDKKGI 180

Query: 181  MDFLLNTSNITKDNVEVVSIVGMGGLGKTALAQTVYNDEKINNRFKWKIWVCISQEFDIK 240
            + FLL+T N+ ++NV VV+I+GMGGLGKTALAQ++Y D K N  F+  +WVCIS+EFD+K
Sbjct: 181  VHFLLDT-NVAQENVAVVAIIGMGGLGKTALAQSIYGDMKENKHFELTMWVCISEEFDVK 240

Query: 241  VIVEKILESITKTKQE-SLQLDILQSMLQEKIYGKKYLLVMDDVWNVDHEKWIGLKRFLM 300
            +IVEKI+ES+TK + E +LQLD LQ+ML+EKI GK+YLLVMDDVWNV+ EKWI LK FL 
Sbjct: 241  IIVEKIIESLTKKRPEPNLQLDTLQNMLREKIDGKRYLLVMDDVWNVNREKWINLKAFLT 300

Query: 301  GGASGSKILVTTRNLQTAQASDTVWFHHLKELDKDNSWALFRKMAFLNKEEELENSNLVR 360
            GGA GS+IL+TTR  Q A  S+TV FHHL EL+K++SW LFRKMAF N+ E LENS LV 
Sbjct: 301  GGAKGSRILITTRTHQVAHTSETVLFHHLSELNKNSSWELFRKMAFSNESEVLENSKLVI 360

Query: 361  IGKEIVAKLKGYPLSIRVVGRLLYFKNTEMDWSSFKDNELDSILQEDDQIQPILKISFNH 420
            IGKEIVA LKG PL++RV+G  LY K TE DW SFKDNEL +I+Q++++IQ ILKISFN 
Sbjct: 361  IGKEIVANLKGSPLALRVIGSYLYSKKTEKDWLSFKDNELGTIMQQENEIQSILKISFNQ 420

Query: 421  LPPKLKQCFTYCALFPKDYEFKKNGLVKQWMAQGFIQAHNKKAIEDVGDDYFQELVGRSF 480
            L   LKQCFTYCALFPKDYE +K+ L+KQWMAQGFIQ  NKK +EDVGDDYF+EL+GRSF
Sbjct: 421  LSSGLKQCFTYCALFPKDYEIQKDDLIKQWMAQGFIQPQNKKTMEDVGDDYFKELLGRSF 480

Query: 481  FQDIRKNKWGDLKYCKMHDLLHDLACSIGENECVVVSDDVGSIDKRTRHASFLLSKRLTR 540
            FQDI+KNKWG++K  K+HD +HDLACS+ EN CV+ +DD  +IDKRTRH S       TR
Sbjct: 481  FQDIKKNKWGEIKEFKIHDFMHDLACSVVENVCVLTNDDTKTIDKRTRHVSISTFISKTR 540

Query: 541  -EVVSKSSIEVTSLRTLDIDSRASFRSFKKTCHMNLFQLRTLNLDRCCCHPPKFVDKLKH 600
             +V+++S  E  +LRTL+        +  K    N  QLRTLN+D    H PK + K+KH
Sbjct: 541  WKVITESLKEAKNLRTLNY-------ACDKIDLSNHLQLRTLNVD-FLYHVPKCIGKMKH 600

Query: 601  LRYLNLSGLNVTFLPNSITTLYNLETLILRYCLWLRKLPKDINNLINLRHLDIYDCSS-- 660
            LRY+NLS   + FLP  IT LYNLETLILR C  L  LP DI NLINLRHLDI  C+S  
Sbjct: 601  LRYVNLSNTWIDFLPKFITKLYNLETLILRNCQRLMGLPSDIKNLINLRHLDIMHCNSNC 660

Query: 661  LTHMPKGLGGMTSLQTMSMFVLGKNKGGDLSALNGLKSLRGLLCIKGLQFCTTADLKN-- 720
             + MP GLG MTSLQTM++FVLG   GG+LS LNGL SLRG L IK LQFC T +L+N  
Sbjct: 661  WSSMPMGLGSMTSLQTMNLFVLGAKWGGELSELNGLNSLRGSLRIKQLQFCITPNLENAK 720

Query: 721  ----------LELHWDIKMDHEDALDDGDNDDEGVLEGLKPHSNIRKMIIKGYRGMKLCD 780
                      LELHWD    +    +  +++DE VLE LKPH N++K+ I+GY+G+KLC+
Sbjct: 721  YLEEKSGIQKLELHWD--SSYGIGRNTFNDEDEKVLECLKPHPNLQKIRIEGYKGVKLCN 780

Query: 781  WFSSNFLGGLVSIELSHCEKLEHLPQFDQFLYLKHLLLGYLPNIEYIDSGNSVSSS-TTF 840
            WFS   +G LV+I+L  CEKL+HLPQFDQF +LKHL L  LPNIE+ID  N VSSS TTF
Sbjct: 781  WFSFGSIGSLVTIKLCECEKLKHLPQFDQFPFLKHLHLEDLPNIEFIDDNNYVSSSLTTF 840

Query: 841  FPSLEKLRIESMPKLKGWWKGEI-----SFPTTILHQLSELCIFYCPLLASIPQHPSLES 900
            FPSLEKL I  +PKLK WWKGE       FPTT LH LS L I +CP L SIPQH  L S
Sbjct: 841  FPSLEKLSIIQLPKLKEWWKGEFIDQTTPFPTT-LHHLSRLKINHCPQLGSIPQHGPLRS 900

Query: 901  LRICGVSVQLFQMVIRMATD--LSEHSSSSSTLSKLSFLEIGTIDLEFLPV-ELFCNMTH 960
            L + GVS+QLF++V+ MAT   +    SSSST   LS L I  +D EFL + +LF NMTH
Sbjct: 901  LDVSGVSLQLFELVMEMATTNIIVGQDSSSSTTRSLSSLSIWDMDFEFLQLHDLFSNMTH 960

Query: 961  LESLIIERCKSLQMSSPHPVDEDNDVVWKKLSNLRTLRLESILKLEYFPKSLKYITSLET 1020
            L+SL+I  CK+++MSS       + V+WK+L +LR L L SI +LE  PK L+Y+T+LE 
Sbjct: 961  LKSLVIGNCKNIKMSSSR-----DGVIWKELGSLRRLDLCSIPELECLPKGLQYVTTLEC 1020

Query: 1021 LKLSNCENLVSTEGIGELISLSHLEIDRCPNLPILSEDVGDLISLSHLLIWNCPKLTSLS 1062
            LKL +C NLVS EGI  L SLS LEI+ CPNL    +++G L SLS L I  CP L SL 
Sbjct: 1021 LKLYDCRNLVSIEGIEHLTSLSLLEIEYCPNLISYPQEMGQLTSLSRLRINGCPNLPSLP 1070

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
RGA4_SOLBU3.1e-14034.38Putative disease resistance protein RGA4 OS=Solanum bulbocastanum GN=RGA4 PE=2 S... [more]
RGA1_SOLBU1.2e-13934.34Putative disease resistance protein RGA1 OS=Solanum bulbocastanum GN=RGA1 PE=2 S... [more]
RGA2_SOLBU5.0e-13834.12Disease resistance protein RGA2 OS=Solanum bulbocastanum GN=RGA2 PE=1 SV=1[more]
RGA3_SOLBU5.0e-13832.93Putative disease resistance protein RGA3 OS=Solanum bulbocastanum GN=RGA3 PE=2 S... [more]
R13L1_ARATH4.0e-11931.16Putative disease resistance RPP13-like protein 1 OS=Arabidopsis thaliana GN=RPPL... [more]
Match NameE-valueIdentityDescription
A0A0A0K9P7_CUCSA0.0e+0098.49Uncharacterized protein OS=Cucumis sativus GN=Csa_7G425940 PE=3 SV=1[more]
A0A0A0K9P7_CUCSA3.1e-8688.38Uncharacterized protein OS=Cucumis sativus GN=Csa_7G425940 PE=3 SV=1[more]
A0A0A0K5Z6_CUCSA2.1e-1839.22Uncharacterized protein OS=Cucumis sativus GN=Csa_7G425920 PE=4 SV=1[more]
A0A0D2NDD9_GOSRA7.8e-20738.66Uncharacterized protein OS=Gossypium raimondii GN=B456_005G155200 PE=3 SV=1[more]
A0A0A0KIT2_CUCSA6.6e-20654.28Uncharacterized protein OS=Cucumis sativus GN=Csa_6G375730 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
AT3G14470.12.2e-12031.16 NB-ARC domain-containing disease resistance protein[more]
AT3G14460.11.0e-10130.79 LRR and NB-ARC domains-containing disease resistance protein[more]
AT1G58602.11.6e-7025.70 LRR and NB-ARC domains-containing disease resistance protein[more]
AT3G50950.28.0e-7027.55 HOPZ-ACTIVATED RESISTANCE 1[more]
AT3G46730.17.5e-6829.06 NB-ARC domain-containing disease resistance protein[more]
Match NameE-valueIdentityDescription
gi|778728529|ref|XP_011659434.1|0.0e+0098.95PREDICTED: putative disease resistance protein RGA1 isoform X1 [Cucumis sativus][more]
gi|778728533|ref|XP_011659435.1|0.0e+0098.95PREDICTED: putative disease resistance protein RGA1 isoform X2 [Cucumis sativus][more]
gi|700189869|gb|KGN45102.1|0.0e+0098.49hypothetical protein Csa_7G425940 [Cucumis sativus][more]
gi|700189869|gb|KGN45102.1|4.4e-8688.38hypothetical protein Csa_7G425940 [Cucumis sativus][more]
gi|778728520|ref|XP_011659433.1|3.0e-1839.22PREDICTED: putative disease resistance protein RGA4 [Cucumis sativus][more]
The following terms have been associated with this gene:
Vocabulary: INTERPRO
TermDefinition
IPR002182NB-ARC
IPR027417P-loop_NTPase
Vocabulary: Molecular Function
TermDefinition
GO:0043531ADP binding
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0008150 biological_process
cellular_component GO:0005575 cellular_component
molecular_function GO:0043531 ADP binding

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Cucsa.128100.1Cucsa.128100.1mRNA


Analysis Name: InterPro Annotations of cucumber (Gy14)
Date Performed: 2017-01-17
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR002182NB-ARCPFAMPF00931NB-ARCcoord: 190..458
score: 2.2
IPR027417P-loop containing nucleoside triphosphate hydrolaseGENE3DG3DSA:3.40.50.300coord: 171..344
score: 1.4
IPR027417P-loop containing nucleoside triphosphate hydrolaseunknownSSF52540P-loop containing nucleoside triphosphate hydrolasescoord: 153..430
score: 9.03
NoneNo IPR availableunknownCoilCoilcoord: 35..55
scor
NoneNo IPR availablePRINTSPR00364DISEASERSISTcoord: 196..211
score: 1.2E-18coord: 987..1003
score: 1.2E-18coord: 269..283
score: 1.2E-18coord: 367..381
score: 1.2
NoneNo IPR availablePANTHERPTHR23155LEUCINE-RICH REPEAT-CONTAINING PROTEINcoord: 1034..1083
score: 1.3E-274coord: 3..765
score: 1.3E
NoneNo IPR availablePANTHERPTHR23155:SF563SUBFAMILY NOT NAMEDcoord: 1034..1083
score: 1.3E-274coord: 3..765
score: 1.3E

The following gene(s) are paralogous to this gene:

None