BLAST of Cucsa.128100 vs. Swiss-Prot
Match:
RGA4_SOLBU (Putative disease resistance protein RGA4 OS=Solanum bulbocastanum GN=RGA4 PE=2 SV=1)
HSP 1 Score: 501.1 bits (1289), Expect = 3.1e-140
Identity = 349/1015 (34.38%), Postives = 522/1015 (51.43%), Query Frame = 1
Query: 28 LWGVNDELDKLQNTLSAIKAVLLDAEEQQSKSHTVKDWIAKIKDVFYDIDDLIDEFSYET 87
++G E +KL + S I+AVL DA+E+Q K +++W+ K+ Y++DD++ E E
Sbjct: 24 IFGFEKECEKLSSVFSTIQAVLQDAQEKQLKDKAIENWLQKLNSAAYEVDDILGECKNEA 83
Query: 88 LRRQVLTKDRTITKQVRIFFSKSNQIAFGFKMGQTIKKVREKLDAIAAIKAQLHLSVCAR 147
+R + Q R+ F I F K+G+ +K++ EKLDAI+ + + H +
Sbjct: 84 IRFE----------QSRLGFYHPGIINFRHKIGRRMKEIMEKLDAISEERRKFHF--LEK 143
Query: 148 EVRDNEPRKVRETSSFIPEGEIIGRDEDRKSVMDFLLNTSNITKDNVEVVSIVGMGGLGK 207
RET + E ++ GRD++ ++ L+N N+ ++ + V I+GMGGLGK
Sbjct: 144 ITERQAAAATRETGFVLTEPKVYGRDKEEDEIVKILINNVNVAEE-LPVFPIIGMGGLGK 203
Query: 208 TALAQTVYNDEKINNRFKWKIWVCISQEFDIKVIVEKILESITKTKQESLQLDILQSMLQ 267
T LAQ ++NDE++ F KIWVC+S +FD K +++ I+ +I ++ L Q LQ
Sbjct: 204 TTLAQMIFNDERVTKHFNPKIWVCVSDDFDEKRLIKTIIGNIERSSPHVEDLASFQKKLQ 263
Query: 268 EKIYGKKYLLVMDDVWNVDHEKWIGLKRFLMGGASGSKILVTTRNLQTAQASDTVWFHHL 327
E + GK+YLLV+DDVWN D EKW L+ L GA G+ IL TTR + T+ +HL
Sbjct: 264 ELLNGKRYLLVLDDVWNDDLEKWAKLRAVLTVGARGASILATTRLEKVGSIMGTLQPYHL 323
Query: 328 KELDKDNSWALFRKMAFLNKEEELENSNLVRIGKEIVAKLKGYPLSIRVVGRLLYFKNTE 387
L +S LF + AF ++E N NLV IGKEIV K G PL+ + +G LL FK E
Sbjct: 324 SNLSPHDSLLLFMQRAFGQQKEA--NPNLVAIGKEIVKKCGGVPLAAKTLGGLLRFKREE 383
Query: 388 MDWSSFKDNELDSILQEDDQIQPILKISFNHLPPKLKQCFTYCALFPKDYEFKKNGLVKQ 447
+W +DNE+ S+ Q++ I P L++S++HLP L+QCF YCA+FPKD + K L+
Sbjct: 384 SEWEHVRDNEIWSLPQDESSILPALRLSYHHLPLDLRQCFAYCAVFPKDTKMIKENLITL 443
Query: 448 WMAQGFIQAHNKKAIEDVGDDYFQELVGRSFFQDIRKNKWGDLKYCKMHDLLHDLACSIG 507
WMA GF+ + +EDVG++ + EL RSFFQ+I + K G+ Y K+HDL+HDLA S+
Sbjct: 444 WMAHGFLLSKGNLELEDVGNEVWNELYLRSFFQEI-EAKSGN-TYFKIHDLIHDLATSL- 503
Query: 508 ENECVVVSDDVGSIDKRTRHASFLLSKRLTREVVSKSSIEVTSLRTLDIDSRASFRSFKK 567
S G+I RE+ K S+ + S S KK
Sbjct: 504 ----FSASASCGNI----------------REINVKDYKHTVSIGFAAVVSSYSPSLLKK 563
Query: 568 TCHMNLFQLRTLNLDRCCCHPPKFVDKLKHLRYLNLSGLNVTFLPNSITTLYNLETLILR 627
+ + L L++ P + L HLRYL+LS N LP + L NL+TL +
Sbjct: 564 FVSLRVLNLSYSKLEQL----PSSIGDLLHLRYLDLSCNNFRSLPERLCKLQNLQTLDVH 623
Query: 628 YCLWLRKLPKDINNLINLRHLDIYDCSSLTHMPKGLGGMTSLQTMSMFVLGKNKGGDLSA 687
C L LPK + L +LRHL + C LT P +G +T L+T+ F++G KG L
Sbjct: 624 NCYSLNCLPKQTSKLSSLRHLVVDGC-PLTSTPPRIGLLTCLKTLGFFIVGSKKGYQLGE 683
Query: 688 LNGLKSLRGLLCIKGLQFCTTADLKNLELHWDIKMDHEDALDDGDND--------DEGVL 747
L L +L G + I L+ + + E + K + + DND + VL
Sbjct: 684 LKNL-NLCGSISITHLE--RVKNDTDAEANLSAKANLQSLSMSWDNDGPNRYESKEVKVL 743
Query: 748 EGLKPHSNIRKMIIKGYRGMKLCDWFSSNFLGGLVSIELSHCEKLEHLPQFDQFLYLKHL 807
E LKPH N++ + I + G + W + + L ++S+ + C+ LP F + L++L
Sbjct: 744 EALKPHPNLKYLEIIAFGGFRFPSWINHSVLEKVISVRIKSCKNCLCLPPFGELPCLENL 803
Query: 808 -LLGYLPNIEYI--DSGNSVSSSTTFFPSLEKLRIESMPKLKGWWK--GEISFPTTILHQ 867
L +EY+ D +S S+ FPSL+KLRI LKG K GE FP
Sbjct: 804 ELQNGSAEVEYVEEDDVHSRFSTRRSFPSLKKLRIWFFRSLKGLMKEEGEEKFP-----M 863
Query: 868 LSELCIFYCPLLASIPQHPSLESLRICGVSVQLFQMVIRMATDLSEHSSSSSTLSKLSFL 927
L E+ I YCPL P S++ L + G + SS S LS L+ L
Sbjct: 864 LEEMAILYCPLFV-FPTLSSVKKLEVHG-------------NTNTRGLSSISNLSTLTSL 923
Query: 928 EIG-TIDLEFLPVELFCNMTHLESLIIERCKSLQMSSPHPVDEDNDVVWKKLSNLRTLRL 987
IG LP E+F ++T+LE L K+L +D L+ L+ L++
Sbjct: 924 RIGANYRATSLPEEMFTSLTNLEFLSFFDFKNL---------KDLPTSLTSLNALKRLQI 964
Query: 988 ESILKLEYFP-KSLKYITSLETLKLSNCENL-VSTEGIGELISLSHLEIDRCPNL 1027
ES LE FP + L+ +TSL L + C+ L EG+ L +L++L + CP +
Sbjct: 984 ESCDSLESFPEQGLEGLTSLTQLFVKYCKMLKCLPEGLQHLTALTNLGVSGCPEV 964
HSP 2 Score: 49.3 bits (116), Expect = 3.2e-04
Identity = 41/105 (39.05%), Postives = 54/105 (51.43%), Query Frame = 1
Query: 963 KKLSNLRTLRLESILKLEYFPKSLKYITSLETLKLSNCENLVST-EGIGELISLSHLEID 1022
KK +LR L L S KLE P S+ + L L LS C N S E + +L +L L++
Sbjct: 524 KKFVSLRVLNL-SYSKLEQLPSSIGDLLHLRYLDLS-CNNFRSLPERLCKLQNLQTLDVH 583
Query: 1023 RCPNLPILSEDVGDLISLSHLLIWNCPKLTSLSEGITRLTSLSSL 1067
C +L L + L SL HL++ CP LTS I LT L +L
Sbjct: 584 NCYSLNCLPKQTSKLSSLRHLVVDGCP-LTSTPPRIGLLTCLKTL 625
HSP 3 Score: 47.4 bits (111), Expect = 1.2e-03
Identity = 34/113 (30.09%), Postives = 58/113 (51.33%), Query Frame = 1
Query: 965 LSNLRTLRLESILKLEYFPKSL-KYITSLETLKLSNCENLVSTE-GIGELISLSHLEIDR 1024
LS L +LR+ + + P+ + +T+LE L + +NL + L +L L+I+
Sbjct: 852 LSTLTSLRIGANYRATSLPEEMFTSLTNLEFLSFFDFKNLKDLPTSLTSLNALKRLQIES 911
Query: 1025 CPNLPILSED-VGDLISLSHLLIWNCPKLTSLSEGITRLTSLSSLCLEDCPNL 1075
C +L E + L SL+ L + C L L EG+ LT+L++L + CP +
Sbjct: 912 CDSLESFPEQGLEGLTSLTQLFVKYCKMLKCLPEGLQHLTALTNLGVSGCPEV 964
BLAST of Cucsa.128100 vs. Swiss-Prot
Match:
RGA1_SOLBU (Putative disease resistance protein RGA1 OS=Solanum bulbocastanum GN=RGA1 PE=2 SV=2)
HSP 1 Score: 499.2 bits (1284), Expect = 1.2e-139
Identity = 353/1028 (34.34%), Postives = 536/1028 (52.14%), Query Frame = 1
Query: 13 VITKLGSSALRELGSLWGVNDELDKLQNTLSAIKAVLLDAEEQQSKSHTVKDWIAKIKDV 72
V+ L S EL L+G DE +L + S I+AVL DA+E+Q +++W+ K+
Sbjct: 9 VLDNLTSFLKGELVLLFGFQDEFQRLSSMFSTIQAVLEDAQEKQLNDKPLENWLQKLNAA 68
Query: 73 FYDIDDLIDEFSYETLRRQVLTKDRTITKQVRIFFSKSNQ-----IAFGFKMGQTIKKVR 132
Y++DD++DE+ TK R S+ + I F K+G+ + +V
Sbjct: 69 TYEVDDILDEYK---------------TKATRFLQSEYGRYHPKVIPFRHKVGKRMDQVM 128
Query: 133 EKLDAIAAIKAQLHLSVCAREVRDNEPRKVRETSSFIPEGEIIGRDEDRKSVMDFLLNTS 192
+KL+AIA + + HL +E RET S + E ++ GRD+++ ++ L+NT+
Sbjct: 129 KKLNAIAEERKKFHL----QEKIIERQAATRETGSVLTEPQVYGRDKEKDEIVKILINTA 188
Query: 193 NITKDNVEVVSIVGMGGLGKTALAQTVYNDEKINNRFKWKIWVCISQEFDIKVIVEKILE 252
+ + + V+ I+GMGGLGKT L+Q V+ND+++ RF KIW+CIS +F+ K +++ I+E
Sbjct: 189 SDAQ-KLSVLPILGMGGLGKTTLSQMVFNDQRVTERFYPKIWICISDDFNEKRLIKAIVE 248
Query: 253 SITKTKQESLQLDILQSMLQEKIYGKKYLLVMDDVWNVDHEKWIGLKRFLMGGASGSKIL 312
SI + L LQ LQE + GK+Y LV+DDVWN D KW L+ L GASG+ +L
Sbjct: 249 SIEGKSLSDMDLAPLQKKLQELLNGKRYFLVLDDVWNEDQHKWANLRAVLKVGASGAFVL 308
Query: 313 VTTRNLQTAQASDTVWFHHLKELDKDNSWALFRKMAFLNKEEELENSNLVRIGKEIVAKL 372
TTR + T+ + L L ++ W LF + AF ++EE N NL+ IGKEIV K
Sbjct: 309 TTTRLEKVGSIMGTLQPYELSNLSPEDCWFLFMQRAFGHQEE--INPNLMAIGKEIVKKC 368
Query: 373 KGYPLSIRVVGRLLYFKNTEMDWSSFKDNELDSILQEDDQIQPILKISFNHLPPKLKQCF 432
G PL+ + +G +L FK E +W +D+ + ++ Q++ I P L++S++HLP L+QCF
Sbjct: 369 GGVPLAAKTLGGILRFKREEREWEHVRDSPIWNLPQDESSILPALRLSYHHLPLDLRQCF 428
Query: 433 TYCALFPKDYEFKKNGLVKQWMAQGFIQAHNKKAIEDVGDDYFQELVGRSFFQDIRKNKW 492
YCA+FPKD + K L+ WMA GF+ + +EDVG++ + EL RSFFQ+I
Sbjct: 429 VYCAVFPKDTKMAKENLIAFWMAHGFLLSKGNLELEDVGNEVWNELYLRSFFQEIEVE-- 488
Query: 493 GDLKYCKMHDLLHDLACSIGENECVVVSDDVGSIDKRTRHASFLLSKRLTREVVSKSSIE 552
Y KMHDL+HDLA S+ S S RE+ +
Sbjct: 489 SGKTYFKMHDLIHDLATSL---------------------FSANTSSSNIREINANYDGY 548
Query: 553 VTSLRTLDIDSRASFRSFKKTCHMNLFQLRTLNLDRCCCHPPKFVDKLKHLRYLNLSG-L 612
+ S+ ++ S S +K + + LR NL++ P + L HLRYL+LSG
Sbjct: 549 MMSIGFAEVVSSYSPSLLQKFVSLRVLNLRNSNLNQL----PSSIGDLVHLRYLDLSGNF 608
Query: 613 NVTFLPNSITTLYNLETLILRYCLWLRKLPKDINNLINLRHLDIYDCSSLTHMPKGLGGM 672
+ LP + L NL+TL L YC L LPK + L +LR+L + D SLT P +G +
Sbjct: 609 RIRNLPKRLCKLQNLQTLDLHYCDSLSCLPKQTSKLGSLRNL-LLDGCSLTSTPPRIGLL 668
Query: 673 TSLQTMSMFVLGKNKGGDLSALNGLKSLRGLLCIKGLQFC---TTADLKNLELHWDI-KM 732
T L+++S FV+GK KG L L L +L G + I L T A NL ++ +
Sbjct: 669 TCLKSLSCFVIGKRKGHQLGELKNL-NLYGSISITKLDRVKKDTDAKEANLSAKANLHSL 728
Query: 733 DHEDALDDGDNDDEGVLEGLKPHSNIRKMIIKGYRGMKLCDWFSSNFLGGLVSIELSHCE 792
LD D VLE LKPHSN++ + I G+ G++L DW + + L +VSI + CE
Sbjct: 729 CLSWDLDGKHRYDSEVLEALKPHSNLKYLEINGFGGIRLPDWMNQSVLKNVVSIRIRGCE 788
Query: 793 KLEHLPQFDQFLYLKHLLLGY-LPNIEYIDSGNSVSSSTTFFPSLEKLRIESMPKLKGWW 852
LP F + L+ L L ++EY++ FPSL KL I LKG
Sbjct: 789 NCSCLPPFGELPCLESLELHTGSADVEYVEDNVHPGR----FPSLRKLVIWDFSNLKGLL 848
Query: 853 K--GEISFPTTILHQLSELCIFYCPLLASIPQHPSLESLRICGVSVQLFQMVIRMATDLS 912
K GE FP L E+ ++CP+ IP S+++L++ ++ AT L
Sbjct: 849 KMEGEKQFPV-----LEEMTFYWCPMFV-IPTLSSVKTLKV----------IVTDATVL- 908
Query: 913 EHSSSSSTLSKLSFLEIG-TIDLEFLPVELFCNMTHLESLIIERCKSLQMSSPHPVDEDN 972
S S L L+ L+I ++ LP E+F ++ +L+ L I ++L ++
Sbjct: 909 ---RSISNLRALTSLDISDNVEATSLPEEMFKSLANLKYLKISFFRNL---------KEL 952
Query: 973 DVVWKKLSNLRTLRLESILKLEYFP-KSLKYITSLETLKLSNCENL-VSTEGIGELISLS 1025
L+ L++L+ E LE P + +K +TSL L +SNC L EG+ L +L+
Sbjct: 969 PTSLASLNALKSLKFEFCDALESLPEEGVKGLTSLTELSVSNCMMLKCLPEGLQHLTALT 952
HSP 2 Score: 51.2 bits (121), Expect = 8.5e-05
Identity = 35/112 (31.25%), Postives = 54/112 (48.21%), Query Frame = 1
Query: 961 VWKKLSNLRTLRLESILKLEYFPKSLKYITSLETLKLSNCENLVSTEGIGELISLSHLEI 1020
++K L+NL+ L++ L+ P SL + +L++LK C+ L S
Sbjct: 863 MFKSLANLKYLKISFFRNLKELPTSLASLNALKSLKFEFCDALES--------------- 922
Query: 1021 DRCPNLPILSEDVGDLISLSHLLIWNCPKLTSLSEGITRLTSLSSLCLEDCP 1073
LP E V L SL+ L + NC L L EG+ LT+L++L + CP
Sbjct: 923 -----LP--EEGVKGLTSLTELSVSNCMMLKCLPEGLQHLTALTTLTITQCP 952
HSP 3 Score: 48.1 bits (113), Expect = 7.2e-04
Identity = 55/181 (30.39%), Postives = 82/181 (45.30%), Query Frame = 1
Query: 953 PVDEDNDVVWKKLSNLRTLRLESILKLEYFPKS-LKYITSLETL-KLSNCENLVST---- 1012
PV E+ W + + TL LK+ + L+ I++L L L +N+ +T
Sbjct: 802 PVLEEMTFYWCPMFVIPTLSSVKTLKVIVTDATVLRSISNLRALTSLDISDNVEATSLPE 861
Query: 1013 EGIGELISLSHLEIDRCPNLPILSEDVGDLISLSHLLIWNCPKLTSL-SEGITRLTSLSS 1072
E L +L +L+I NL L + L +L L C L SL EG+ LTSL+
Sbjct: 862 EMFKSLANLKYLKISFFRNLKELPTSLASLNALKSLKFEFCDALESLPEEGVKGLTSLTE 921
Query: 1073 LCLEDCPNLVSLPQEFLHHHSSLPGGRFLRILNCPKLQIQDKKQKEEEEEDQEDWNELIH 1127
L + +C L LP E L H ++L L I CP + K E EDW+++ H
Sbjct: 922 LSVSNCMMLKCLP-EGLQHLTAL---TTLTITQCPIV------FKRCERGIGEDWHKIAH 972
BLAST of Cucsa.128100 vs. Swiss-Prot
Match:
RGA2_SOLBU (Disease resistance protein RGA2 OS=Solanum bulbocastanum GN=RGA2 PE=1 SV=1)
HSP 1 Score: 493.8 bits (1270), Expect = 5.0e-138
Identity = 349/1023 (34.12%), Postives = 534/1023 (52.20%), Query Frame = 1
Query: 13 VITKLGSSALRELGSLWGVNDELDKLQNTLSAIKAVLLDAEEQQSKSHTVKDWIAKIKDV 72
++ L S EL L+G DE +L + S I+AVL DA+E+Q + +++W+ K+
Sbjct: 9 LLDNLTSFLKGELVLLFGFQDEFQRLSSMFSTIQAVLEDAQEKQLNNKPLENWLQKLNAA 68
Query: 73 FYDIDDLIDEFSYETLRRQVLTKDRTITKQVRIFFSKSNQIAFGFKMGQTIKKVREKLDA 132
Y++DD++DE+ + R R K I F K+G+ + +V +KL A
Sbjct: 69 TYEVDDILDEYKTKATRFSQSEYGRYHPKV----------IPFRHKVGKRMDQVMKKLKA 128
Query: 133 IAAIKAQLHLSVCAREVRDNEPRKVRETSSFIPEGEIIGRDEDRKSVMDFLLNTSNITKD 192
IA + HL ++ + + + RET S + E ++ GRD+++ ++ L+N + +
Sbjct: 129 IAEERKNFHLH---EKIVERQAVR-RETGSVLTEPQVYGRDKEKDEIVKILINNVSDAQ- 188
Query: 193 NVEVVSIVGMGGLGKTALAQTVYNDEKINNRFKWKIWVCISQEFDIKVIVEKILESIT-K 252
++ V+ I+GMGGLGKT LAQ V+ND+++ F KIW+C+S++FD K +++ I+ESI +
Sbjct: 189 HLSVLPILGMGGLGKTTLAQMVFNDQRVTEHFHSKIWICVSEDFDEKRLIKAIVESIEGR 248
Query: 253 TKQESLQLDILQSMLQEKIYGKKYLLVMDDVWNVDHEKWIGLKRFLMGGASGSKILVTTR 312
+ L LQ LQE + GK+YLLV+DDVWN D +KW L+ L GASG+ +L TTR
Sbjct: 249 PLLGEMDLAPLQKKLQELLNGKRYLLVLDDVWNEDQQKWANLRAVLKVGASGASVLTTTR 308
Query: 313 NLQTAQASDTVWFHHLKELDKDNSWALFRKMAFLNKEEELENSNLVRIGKEIVAKLKGYP 372
+ T+ + L L +++ W LF + AF ++EE N NLV IGKEIV K G P
Sbjct: 309 LEKVGSIMGTLQPYELSNLSQEDCWLLFMQRAFGHQEEI--NPNLVAIGKEIVKKSGGVP 368
Query: 373 LSIRVVGRLLYFKNTEMDWSSFKDNELDSILQEDDQIQPILKISFNHLPPKLKQCFTYCA 432
L+ + +G +L FK E W +D+ + ++ Q++ I P L++S++ LP LKQCF YCA
Sbjct: 369 LAAKTLGGILCFKREERAWEHVRDSPIWNLPQDESSILPALRLSYHQLPLDLKQCFAYCA 428
Query: 433 LFPKDYEFKKNGLVKQWMAQGFIQAHNKKAIEDVGDDYFQELVGRSFFQDIRKNKWGDLK 492
+FPKD + +K L+ WMA GF+ + +EDVGD+ ++EL RSFFQ+I
Sbjct: 429 VFPKDAKMEKEKLISLWMAHGFLLSKGNMELEDVGDEVWKELYLRSFFQEIEVKD--GKT 488
Query: 493 YCKMHDLLHDLACSIGENECVVVSDDVGSIDKRTRHASFLLSKRLTREVVSKSSIEVTSL 552
Y KMHDL+HDLA S+ S + S + R ++K ++S EV
Sbjct: 489 YFKMHDLIHDLATSL-------FSANTSSSNIRE------INKHSYTHMMSIGFAEVVFF 548
Query: 553 RTLDIDSRASFRSFKKTCHMNLFQLRTLNL-DRCCCHPPKFVDKLKHLRYLNLSGLNVTF 612
TL + LR LNL D P + L HLRYLNL G +
Sbjct: 549 YTLPPLEK-------------FISLRVLNLGDSTFNKLPSSIGDLVHLRYLNLYGSGMRS 608
Query: 613 LPNSITTLYNLETLILRYCLWLRKLPKDINNLINLRHLDIYDCSSLTHMPKGLGGMTSLQ 672
LP + L NL+TL L+YC L LPK+ + L +LR+L + SLT MP +G +T L+
Sbjct: 609 LPKQLCKLQNLQTLDLQYCTKLCCLPKETSKLGSLRNLLLDGSQSLTCMPPRIGSLTCLK 668
Query: 673 TMSMFVLGKNKGGDLSALNGLKSLRGLLCIKGLQ------------FCTTADLKNLELHW 732
T+ FV+G+ KG L L L +L G + I L+ +L +L + W
Sbjct: 669 TLGQFVVGRKKGYQLGELGNL-NLYGSIKISHLERVKNDKDAKEANLSAKGNLHSLSMSW 728
Query: 733 DIKMDHEDALDDGDNDDEGVLEGLKPHSNIRKMIIKGYRGMKLCDWFSSNFLGGLVSIEL 792
+ H ++++ VLE LKPHSN+ + I G+RG+ L +W + + L +VSI +
Sbjct: 729 NNFGPHIY-----ESEEVKVLEALKPHSNLTSLKIYGFRGIHLPEWMNHSVLKNIVSILI 788
Query: 793 SHCEKLEHLPQFDQFLYLKHLLLGY-LPNIEYIDSGN----SVSSSTTFFPSLEKLRIES 852
S+ LP F L+ L L + ++EY++ + S + FPSL KL I
Sbjct: 789 SNFRNCSCLPPFGDLPCLESLELHWGSADVEYVEEVDIDVHSGFPTRIRFPSLRKLDIWD 848
Query: 853 MPKLKGWWK--GEISFPTTILHQLSELCIFYCPLLASIPQHPSLESLRICGVSVQLFQMV 912
LKG K GE FP L E+ I CP L +L SLRIC
Sbjct: 849 FGSLKGLLKKEGEEQFPV-----LEEMIIHECPFLTLSSNLRALTSLRIC---------Y 908
Query: 913 IRMATDLSEHSSSSSTLSKLSFLEIGTID-LEFLPVELFCNMTHLESLIIERCKSLQMSS 972
++AT E + L+ L +L I + L+ LP L ++ L+SL I+ C +L+
Sbjct: 909 NKVATSFPEEMFKN--LANLKYLTISRCNNLKELPTSL-ASLNALKSLKIQLCCALESLP 955
Query: 973 PHPVDEDNDVVWKKLSNLRTLRLESILKLEYFPKSLKYITSLETLKLSNCENLVS--TEG 1012
++ LS+L L +E L+ P+ L+++T+L +LK+ C L+ +G
Sbjct: 969 EEGLEG--------LSSLTELFVEHCNMLKCLPEGLQHLTTLTSLKIRGCPQLIKRCEKG 955
HSP 2 Score: 60.5 bits (145), Expect = 1.4e-07
Identity = 109/454 (24.01%), Postives = 190/454 (41.85%), Query Frame = 1
Query: 639 INNLINLRHLDIYDCSSLTHMPKGLGGMTSLQTMSMFVLG-KNKGGDLSALNGLKSLRGL 698
+ I+LR L++ D S+ +P +G + L+ ++++ G ++ L L L++L
Sbjct: 522 LEKFISLRVLNLGD-STFNKLPSSIGDLVHLRYLNLYGSGMRSLPKQLCKLQNLQTLDLQ 581
Query: 699 LCIKGLQFCTTADLKNLELHWDIKMDHEDALDDGDNDDEGVLEGLKPHSNIRKMIIKGYR 758
C K C + L ++ +D +L G L LK + + ++ +
Sbjct: 582 YCTK--LCCLPKETSKLGSLRNLLLDGSQSLTCMP-PRIGSLTCLK---TLGQFVVGRKK 641
Query: 759 GMKLCDWFSSNFLGGLVSIELSHCEKLEHLPQFDQFLYLKHLLLGYLPNIEYIDSGNSVS 818
G +L + + N G SI++SH E++++ + L L ++ + + G +
Sbjct: 642 GYQLGELGNLNLYG---SIKISHLERVKNDKDAKEANLSAKGNLHSL-SMSWNNFGPHIY 701
Query: 819 SSTTFFPSLEKLRIES-MPKLKGWWKGEISFPTTILHQLSELCIFY-------CPLLASI 878
S LE L+ S + LK + I P + H + + + C L
Sbjct: 702 ESEEV-KVLEALKPHSNLTSLKIYGFRGIHLPEWMNHSVLKNIVSILISNFRNCSCLPPF 761
Query: 879 PQHPSLESLRICGVSVQLFQMVIRMATDLSEHSSSSS-----TLSKLSFLEIGTIDLEFL 938
P LESL + S + + D+ HS + +L KL + G++ L
Sbjct: 762 GDLPCLESLELHWGSADVEYVE---EVDIDVHSGFPTRIRFPSLRKLDIWDFGSLK-GLL 821
Query: 939 PVELFCNMTHLESLIIERCKSLQMSSPHPVDEDNDVVWKKLSNLRTLRLESILKLEYFPK 998
E LE +II C L +SS L L +LR+ FP+
Sbjct: 822 KKEGEEQFPVLEEMIIHECPFLTLSS-------------NLRALTSLRICYNKVATSFPE 881
Query: 999 SL-KYITSLETLKLSNCENLVSTE-GIGELISLSHLEIDRCPNLPILSED-VGDLISLSH 1058
+ K + +L+ L +S C NL + L +L L+I C L L E+ + L SL+
Sbjct: 882 EMFKNLANLKYLTISRCNNLKELPTSLASLNALKSLKIQLCCALESLPEEGLEGLSSLTE 941
Query: 1059 LLIWNCPKLTSLSEGITRLTSLSSLCLEDCPNLV 1076
L + +C L L EG+ LT+L+SL + CP L+
Sbjct: 942 LFVEHCNMLKCLPEGLQHLTTLTSLKIRGCPQLI 946
HSP 3 Score: 40.0 bits (92), Expect = 2.0e-01
Identity = 43/156 (27.56%), Postives = 63/156 (40.38%), Query Frame = 1
Query: 992 LETLKLSNCENLVSTEGIGELISLSHLEIDRCPNLPILSEDVGDLISLSHLLIWNCPKLT 1051
LE + + C L + + L SL + P E +L +L +L I C L
Sbjct: 816 LEEMIIHECPFLTLSSNLRALTSLRICYNKVATSFP--EEMFKNLANLKYLTISRCNNLK 875
Query: 1052 SLSEGITRLTSLSSLCLEDCPNLVSLPQEFLHHHSS--------------LPGG------ 1111
L + L +L SL ++ C L SLP+E L SS LP G
Sbjct: 876 ELPTSLASLNALKSLKIQLCCALESLPEEGLEGLSSLTELFVEHCNMLKCLPEGLQHLTT 935
Query: 1112 -RFLRILNCPKLQIQDKKQKEEEEEDQEDWNELIHV 1127
L+I CP+L K E+ EDW+++ H+
Sbjct: 936 LTSLKIRGCPQL------IKRCEKGIGEDWHKISHI 963
BLAST of Cucsa.128100 vs. Swiss-Prot
Match:
RGA3_SOLBU (Putative disease resistance protein RGA3 OS=Solanum bulbocastanum GN=RGA3 PE=2 SV=2)
HSP 1 Score: 493.8 bits (1270), Expect = 5.0e-138
Identity = 353/1072 (32.93%), Postives = 532/1072 (49.63%), Query Frame = 1
Query: 24 ELGSLWGVNDELDKLQNTLSAIKAVLLDAEEQQSKSHTVKDWIAKIKDVFYDIDDLIDEF 83
ELG ++G E KL + S I+AVL DA+E+Q K +K+W+ K+ Y++DD++D+
Sbjct: 20 ELGLVFGFEKEFKKLSSMFSMIQAVLEDAQEKQLKYKAIKNWLQKLNVAAYEVDDILDDC 79
Query: 84 SYETLRRQVLTKDRTITKQVRIFFSKSNQIAFGFKMGQTIKKVREKLDAIAAIKAQLHLS 143
E R KQ + I F +K+G+ +K++ EKLDAIA + HL
Sbjct: 80 KTEAAR----------FKQAVLGRYHPRTITFCYKVGKRMKEMMEKLDAIAEERRNFHLD 139
Query: 144 VCAREVRDNEPRKVRETSSFIPEGEIIGRDEDRKSVMDFLLNTSNITKDNVEVVSIVGMG 203
R + R R+T + E ++ GR+++ ++ L+N + +++ V V+ I+GMG
Sbjct: 140 --ERIIERQAAR--RQTGFVLTEPKVYGREKEEDEIVKILINNVSYSEE-VPVLPILGMG 199
Query: 204 GLGKTALAQTVYNDEKINNRFKWKIWVCISQEFDIKVIVEKILESITKTKQESLQLDILQ 263
GLGKT LAQ V+ND++I F KIWVC+S +FD K +++ I+ESI + L LQ
Sbjct: 200 GLGKTTLAQMVFNDQRITEHFNLKIWVCVSDDFDEKRLIKAIVESIEGKSLGDMDLAPLQ 259
Query: 264 SMLQEKIYGKKYLLVMDDVWNVDHEKWIGLKRFLMGGASGSKILVTTRNLQTAQASDTVW 323
LQE + GK+Y LV+DDVWN D EKW L+ L GASG+ IL+TTR + T+
Sbjct: 260 KKLQELLNGKRYFLVLDDVWNEDQEKWDNLRAVLKIGASGASILITTRLEKIGSIMGTLQ 319
Query: 324 FHHLKELDKDNSWALFRKMAFLNKEEELENSNLVRIGKEIVAKLKGYPLSIRVVGRLLYF 383
+ L L +++ W LF++ AF ++ E + L+ IGKEIV K G PL+ + +G LL F
Sbjct: 320 LYQLSNLSQEDCWLLFKQRAFCHQTET--SPKLMEIGKEIVKKCGGVPLAAKTLGGLLRF 379
Query: 384 KNTEMDWSSFKDNELDSILQEDDQIQPILKISFNHLPPKLKQCFTYCALFPKDYEFKKNG 443
K E +W +D+E+ ++ Q+++ + P L++S++HLP L+QCF YCA+FPKD + +K
Sbjct: 380 KREESEWEHVRDSEIWNLPQDENSVLPALRLSYHHLPLDLRQCFAYCAVFPKDTKIEKEY 439
Query: 444 LVKQWMAQGFIQAHNKKAIEDVGDDYFQELVGRSFFQDIRKNKWGDLKYCKMHDLLHDLA 503
L+ WMA F+ + +EDVG++ + EL RSFFQ+I Y KMHDL+HDLA
Sbjct: 440 LIALWMAHSFLLSKGNMELEDVGNEVWNELYLRSFFQEIEVKS--GKTYFKMHDLIHDLA 499
Query: 504 CSIGENECVVVSDDVGSIDKRTRHASFLLSKRLTREVVSKSSIEVTSLRT-----LDIDS 563
S+ S S R R++ K ++ + T + I
Sbjct: 500 TSM---------------------FSASASSRSIRQINVKDDEDMMFIVTNYKDMMSIGF 559
Query: 564 RASFRSFKKTCHMNLFQLRTLNLDRCCCHP-PKFVDKLKHLRYLNLSGLNVTFLPNSITT 623
S+ + LR LNL P V L HLRYL+LSG + LP +
Sbjct: 560 SEVVSSYSPSLFKRFVSLRVLNLSNSEFEQLPSSVGDLVHLRYLDLSGNKICSLPKRLCK 619
Query: 624 LYNLETLILRYCLWLRKLPKDINNLINLRHLDIYDCSSLTHMPKGLGGMTSLQTMSMFVL 683
L NL+TL L C L LPK + L +LR+L + C LT MP +G +T L+T+ FV+
Sbjct: 620 LQNLQTLDLYNCQSLSCLPKQTSKLCSLRNLVLDHCP-LTSMPPRIGLLTCLKTLGYFVV 679
Query: 684 GKNKGGDLSALNGLKSLRGLLCIKGLQ------------FCTTADLKNLELHWDIKMDHE 743
G+ KG L L L +LRG + I L+ A+L +L + WD +E
Sbjct: 680 GERKGYQLGELRNL-NLRGAISITHLERVKNDMEAKEANLSAKANLHSLSMSWDRPNRYE 739
Query: 744 DALDDGDNDDEGVLEGLKPHSNIRKMIIKGYRGMKLCDWFSSNFLGGLVSIELSHCEKLE 803
+++ VLE LKPH N++ + I + G L DW + + L +VSI +S CE
Sbjct: 740 -------SEEVKVLEALKPHPNLKYLEIIDFCGFCLPDWMNHSVLKNVVSILISGCENCS 799
Query: 804 HLPQFDQFLYLKHL-LLGYLPNIEYIDSGNSVSSSTTFFPSLEKLRIESMPKLKGWWKGE 863
LP F + L+ L L +EY++ ++ FPSL KL I LKG +
Sbjct: 800 CLPPFGELPCLESLELQDGSVEVEYVEDSGFLTRRR--FPSLRKLHIGGFCNLKGLQR-- 859
Query: 864 ISFPTTILHQLSELCIFYCPLLASIPQHPSLESLRICGVSVQLFQMVIRMATDLSEHSSS 923
+ Q P LE ++I + +F
Sbjct: 860 ---------------------MKGAEQFPVLEEMKISDCPMFVFP--------------- 919
Query: 924 SSTLSKLSFLEI-GTIDLEFLPVELFCNMTHLESLIIERCKSLQMSSPHPVDEDNDVVWK 983
TLS + LEI G D L ++++L +L SL++ S H V + ++K
Sbjct: 920 --TLSSVKKLEIWGEADAGGLS-----SISNLSTLT-----SLKIFSNHTVTSLLEEMFK 968
Query: 984 KLSNLRTLRLESILKLEYFPKSLKYITSLETLKLSNCENLVSTEGIGELISLSHLEIDRC 1043
L NL L + + L+ P SL + +L+ L + C L S
Sbjct: 980 NLENLIYLSVSFLENLKELPTSLASLNNLKCLDIRYCYALES------------------ 968
Query: 1044 PNLPILSEDVGDLISLSHLLIWNCPKLTSLSEGITRLTSLSSLCLEDCPNLV 1076
LP E + L SL+ L + +C L L EG+ LT+L+SL + CP L+
Sbjct: 1040 --LP--EEGLEGLSSLTELFVEHCNMLKCLPEGLQHLTTLTSLKIRGCPQLI 968
BLAST of Cucsa.128100 vs. Swiss-Prot
Match:
R13L1_ARATH (Putative disease resistance RPP13-like protein 1 OS=Arabidopsis thaliana GN=RPPL1 PE=3 SV=1)
HSP 1 Score: 431.0 bits (1107), Expect = 4.0e-119
Identity = 315/1011 (31.16%), Postives = 515/1011 (50.94%), Query Frame = 1
Query: 35 LDKLQNTLSAIKAVLLDAEEQQSKSHTVKDWIAKIKDVFYDIDDLIDEFSYETLRRQVLT 94
L++L L I AVL+DAEE+Q + V+ W+ +++DV Y +D +D+ + E LR +
Sbjct: 39 LERLSTALLTITAVLIDAEEKQITNPVVEKWVNELRDVVYHAEDALDDIATEALRLNIGA 98
Query: 95 KDRTITK--QVRIFFSKSNQIAFGFKMGQT-IKKVREKLDAIAAIKAQLHLSVCAREVRD 154
+ + + Q+R S + + + +T ++KV +L+ +A+ + L L +E+
Sbjct: 99 ESSSSNRLRQLRGRMSLGDFLDGNSEHLETRLEKVTIRLERLASQRNILGL----KELTA 158
Query: 155 NEPRKVRETSSFIPEGEIIGRDEDRKSVMDFLLNTSNITKDN-VEVVSIVGMGGLGKTAL 214
P++ T+S + E E+ GRD+D+ +M FL+ + KDN + VV+IVG+GG+GKT L
Sbjct: 159 MIPKQRLPTTSLVDESEVFGRDDDKDEIMRFLIPENG--KDNGITVVAIVGIGGVGKTTL 218
Query: 215 AQTVYNDEKINNRFKWKIWVCISQEFDIKVIVEKILESITKTKQESLQLDILQSMLQEKI 274
+Q +YND+ + + F K+W +S+EFD+ I +K+ ES+T E LD+LQ L+E++
Sbjct: 219 SQLLYNDQHVRSYFGTKVWAHVSEEFDVFKITKKVYESVTSRPCEFTDLDVLQVKLKERL 278
Query: 275 YGK--KYLLVMDDVWNVDHEKWIGLKRFLMGGASGSKILVTTRNLQTAQASDTVWFHHLK 334
G +LLV+DD+WN + W L++ + A GS+ILVTTR+ + A V H+L+
Sbjct: 279 TGTGLPFLLVLDDLWNENFADWDLLRQPFIHAAQGSQILVTTRSQRVASIMCAVHVHNLQ 338
Query: 335 ELDKDNSWALFRKMAFLNKEEELENSNLVRIGKEIVAKLKGYPLSIRVVGRLLYFKNTEM 394
L + W+LF K F N+E L N + + + IV K +G PL+++ +G +L F+ +
Sbjct: 339 PLSDGDCWSLFMKTVFGNQEPCL-NREIGDLAERIVHKCRGLPLAVKTLGGVLRFEGKVI 398
Query: 395 DWSSFKDNELDSILQEDDQIQPILKISFNHLPPKLKQCFTYCALFPKDYEFKKNGLVKQW 454
+W + + + + + P+L++S+ +LP LK+CF YC++FPK + F+K+ +V W
Sbjct: 399 EWERVLSSRIWDLPADKSNLLPVLRVSYYYLPAHLKRCFAYCSIFPKGHAFEKDKVVLLW 458
Query: 455 MAQGFI-QAHNKKAIEDVGDDYFQELVGRSFFQDIRKNKWGDLKYCKMHDLLHDLA-CSI 514
MA+GF+ Q + K +E++G++YF EL RS Q + +Y MHD +++LA +
Sbjct: 459 MAEGFLQQTRSSKNLEELGNEYFSELESRSLLQKTK------TRYI-MHDFINELAQFAS 518
Query: 515 GENECVVVSDDVGSIDKRTRHASFLLSKRLTREVVSKSSIEVTSLRT-LDIDSRASFRS- 574
GE + +RTR+ S+ L + ++ EV LRT L + S RS
Sbjct: 519 GEFSSKFEDGCKLQVSERTRYLSY-LRDNYAEPMEFEALREVKFLRTFLPLSLTNSSRSC 578
Query: 575 -----FKKTCHMNLFQLRTLNLD--RCCCHPPKFVDKLKHLRYLNLSGLNVTFLPNSITT 634
+ L +LR L+L + PP F + H R+L+LS + LP S+
Sbjct: 579 CLDQMVSEKLLPTLTRLRVLSLSHYKIARLPPDFFKNISHARFLDLSRTELEKLPKSLCY 638
Query: 635 LYNLETLILRYCLWLRKLPKDINNLINLRHLDIYDCSSLTHMPKGLGGMTSLQTMSMFVL 694
+YNL+TL+L YC L++LP DI+NLINLR+LD+ + L MP+ G + SLQT++ F +
Sbjct: 639 MYNLQTLLLSYCSSLKELPTDISNLINLRYLDLIG-TKLRQMPRRFGRLKSLQTLTTFFV 698
Query: 695 GKNKGGDLSALNGLKSLRGLLCIKGLQ-FCTTAD-----------LKNLELHW--DIKMD 754
+ G +S L GL L G L I LQ AD L+ ++ W
Sbjct: 699 SASDGSRISELGGLHDLHGKLKIVELQRVVDVADAAEANLNSKKHLREIDFVWRTGSSSS 758
Query: 755 HEDALDDGDNDDEGVLEGLKPHSNIRKMIIKGYRGMKLCDWFSSNFLGGLVSIELSHCEK 814
+ ++ V E L+PH +I K+ I+ Y+G + DW S +V I L C+
Sbjct: 759 ENNTNPHRTQNEAEVFEKLRPHRHIEKLAIERYKGRRFPDWLSDPSFSRIVCIRLRECQY 818
Query: 815 LEHLPQFDQFLYLKHLLLGYLPNIE------YIDSGNSVSSSTTFFPSLEKLRIESMPKL 874
LP Q LK L + + ++ Y F SLE LR +++P
Sbjct: 819 CTSLPSLGQLPCLKELHISGMVGLQSIGRKFYFSDQQLRDQDQQPFRSLETLRFDNLPDW 878
Query: 875 KGWWKGEISFPTTILHQLSELCIFYCP-LLASIPQH-PSLESLRICGVSVQLFQMVIRMA 934
+ W ++ + L +L I CP L ++P PSL SL I + FQ
Sbjct: 879 QEWLDVRVT-RGDLFPSLKKLFILRCPELTGTLPTFLPSLISLHIYKCGLLDFQP----- 938
Query: 935 TDLSEHSSSSSTLSKLSFLEIGTIDLEFLPVELFCNMTHLESLIIERCK---SLQMSSPH 994
H S L LS ++ L P+ F N+ LE +++C SL++S+ H
Sbjct: 939 ---DHHEYSYRNLQTLS-IKSSCDTLVKFPLNHFANLDKLE---VDQCTSLYSLELSNEH 998
Query: 995 PVDEDNDVVWKKLSNLRTLRLESILKLEYFPKSLKYITSLETLKLSNCENL 1004
+ + LR LR+ L+ PK L + + ++NC L
Sbjct: 999 ---------LRGPNALRNLRINDCQNLQLLPK-LNALPQNLQVTITNCRYL 1010
BLAST of Cucsa.128100 vs. TrEMBL
Match:
A0A0A0K9P7_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_7G425940 PE=3 SV=1)
HSP 1 Score: 1580.1 bits (4090), Expect = 0.0e+00
Identity = 781/793 (98.49%), Postives = 781/793 (98.49%), Query Frame = 1
Query: 350 ELENSNLVRIGKEIVAKLKGYPLSIRVVGRLLYFKNTEMDWSSFKDNELDSILQEDDQIQ 409
ELENSNLVRIGKEIVAKLKGYPLSIRVVGRLLYFKNTEMDWSSFKDNELDSILQEDDQIQ
Sbjct: 171 ELENSNLVRIGKEIVAKLKGYPLSIRVVGRLLYFKNTEMDWSSFKDNELDSILQEDDQIQ 230
Query: 410 PILKISFNHLPPKLKQCFTYCALFPKDYEFKKNGLVKQWMAQGFIQAHNKKAIEDVGDDY 469
PILKISFNHLPPKLKQCFTYCALFPKDYEFKKNGLVKQWMAQGFIQAHNKKAIEDVGDDY
Sbjct: 231 PILKISFNHLPPKLKQCFTYCALFPKDYEFKKNGLVKQWMAQGFIQAHNKKAIEDVGDDY 290
Query: 470 FQELVGRSFFQDIRKNKWGDLKYCKMHDLLHDLACSIGENECVVVSDDVGSIDKRTRHAS 529
FQELVGRSFFQDIRKNKWGDLKYCKMHDLLHDLACSIGENECVVVSDDVGSIDKRTRHAS
Sbjct: 291 FQELVGRSFFQDIRKNKWGDLKYCKMHDLLHDLACSIGENECVVVSDDVGSIDKRTRHAS 350
Query: 530 FLLSKRLTREVVSKSSIEVTSLRTLDIDSRASFRSFKKTCHMNLFQLRTLNLDRCCCHPP 589
FLLSKRLTREVVSKSSIEVTSLRTLDIDSRASFRSFKKTCHMNLFQLRTLNLDRCCCHPP
Sbjct: 351 FLLSKRLTREVVSKSSIEVTSLRTLDIDSRASFRSFKKTCHMNLFQLRTLNLDRCCCHPP 410
Query: 590 KFVDKLKHLRYLNLSGLNVTFLPNSITTLYNLETLILRYCLWLRKLPKDINNLINLRHLD 649
KFVDKLKHLRYLNLSGLNVTFLPNSITTLYNLETLILRYCLWLRKLPKDINNLINLRHLD
Sbjct: 411 KFVDKLKHLRYLNLSGLNVTFLPNSITTLYNLETLILRYCLWLRKLPKDINNLINLRHLD 470
Query: 650 IYDCSSLTHMPKGLGGMTSLQTMSMFVLGKNKGGDLSALNGLKSLRGLLCIKGLQFCTTA 709
IYDCSSLTHMPKGLGGMTSLQTMSMFVLGKNKGGDLSALNGLKSLRGLLCIKGLQFCTTA
Sbjct: 471 IYDCSSLTHMPKGLGGMTSLQTMSMFVLGKNKGGDLSALNGLKSLRGLLCIKGLQFCTTA 530
Query: 710 DLKN------------LELHWDIKMDHEDALDDGDNDDEGVLEGLKPHSNIRKMIIKGYR 769
DLKN LELHWDIKMDHEDALDDGDNDDEGVLEGLKPHSNIRKMIIKGYR
Sbjct: 531 DLKNVSYLKEMYGIQKLELHWDIKMDHEDALDDGDNDDEGVLEGLKPHSNIRKMIIKGYR 590
Query: 770 GMKLCDWFSSNFLGGLVSIELSHCEKLEHLPQFDQFLYLKHLLLGYLPNIEYIDSGNSVS 829
GMKLCDWFSSNFLGGLVSIELSHCEKLEHLPQFDQFLYLKHLLLGYLPNIEYIDSGNSVS
Sbjct: 591 GMKLCDWFSSNFLGGLVSIELSHCEKLEHLPQFDQFLYLKHLLLGYLPNIEYIDSGNSVS 650
Query: 830 SSTTFFPSLEKLRIESMPKLKGWWKGEISFPTTILHQLSELCIFYCPLLASIPQHPSLES 889
SSTTFFPSLEKLRIESMPKLKGWWKGEISFPTTILHQLSELCIFYCPLLASIPQHPSLES
Sbjct: 651 SSTTFFPSLEKLRIESMPKLKGWWKGEISFPTTILHQLSELCIFYCPLLASIPQHPSLES 710
Query: 890 LRICGVSVQLFQMVIRMATDLSEHSSSSSTLSKLSFLEIGTIDLEFLPVELFCNMTHLES 949
LRICGVSVQLFQMVIRMATDLSEHSSSSSTLSKLSFLEIGTIDLEFLPVELFCNMTHLES
Sbjct: 711 LRICGVSVQLFQMVIRMATDLSEHSSSSSTLSKLSFLEIGTIDLEFLPVELFCNMTHLES 770
Query: 950 LIIERCKSLQMSSPHPVDEDNDVVWKKLSNLRTLRLESILKLEYFPKSLKYITSLETLKL 1009
LIIERCKSLQMSSPHPVDEDNDVVWKKLSNLRTLRLESILKLEYFPKSLKYITSLETLKL
Sbjct: 771 LIIERCKSLQMSSPHPVDEDNDVVWKKLSNLRTLRLESILKLEYFPKSLKYITSLETLKL 830
Query: 1010 SNCENLVSTEGIGELISLSHLEIDRCPNLPILSEDVGDLISLSHLLIWNCPKLTSLSEGI 1069
SNCENLVSTEGIGELISLSHLEIDRCPNLPILSEDVGDLISLSHLLIWNCPKLTSLSEGI
Sbjct: 831 SNCENLVSTEGIGELISLSHLEIDRCPNLPILSEDVGDLISLSHLLIWNCPKLTSLSEGI 890
Query: 1070 TRLTSLSSLCLEDCPNLVSLPQEFLHHHSSLPGGRFLRILNCPKLQIQDKKQKEEEEEDQ 1129
TRLTSLSSLCLEDCPNLVSLPQEFLHHHSSLPGGRFLRILNCPKLQIQDKKQKEEEEEDQ
Sbjct: 891 TRLTSLSSLCLEDCPNLVSLPQEFLHHHSSLPGGRFLRILNCPKLQIQDKKQKEEEEEDQ 950
Query: 1130 EDWNELIHVLTGC 1131
EDWNELIHVLTGC
Sbjct: 951 EDWNELIHVLTGC 963
BLAST of Cucsa.128100 vs. TrEMBL
Match:
A0A0A0K9P7_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_7G425940 PE=3 SV=1)
HSP 1 Score: 328.6 bits (841), Expect = 3.1e-86
Identity = 175/198 (88.38%), Postives = 179/198 (90.40%), Query Frame = 1
Query: 1 MADSVLFNVAASVITKLGSSALRELGSLWGVNDELDKLQNTLSAIKAVLLDAEEQQSKSH 60
MADSVLFNVAASVITKLGSSALRELGSLWGVNDELDKLQNTLSAIKAVLLDAEEQQSKSH
Sbjct: 1 MADSVLFNVAASVITKLGSSALRELGSLWGVNDELDKLQNTLSAIKAVLLDAEEQQSKSH 60
Query: 61 TVKDWIAKIKDVFYDIDDLIDEFSYETLRRQVLTKDRTITKQVRIFFSKSNQIAFGFKMG 120
TVKDWIAKIKDVFYDIDDLIDEFSYETLRRQVLTKDRTITKQVRIFFSKSNQIAFGFKMG
Sbjct: 61 TVKDWIAKIKDVFYDIDDLIDEFSYETLRRQVLTKDRTITKQVRIFFSKSNQIAFGFKMG 120
Query: 121 QTIKKVREKLDAIAAIKAQLHLSVCAREVRDNEPRKVRETSSFIPEGEIIGRDEDRKSVM 180
QTIKKVREKLDAIAAIKAQLHLSVCAREVRDNEPRKVRETSSFIPEGEII
Sbjct: 121 QTIKKVREKLDAIAAIKAQLHLSVCAREVRDNEPRKVRETSSFIPEGEIIE--------- 180
Query: 181 DFLLNTSNITKDNVEVVS 199
L SN+ + E+V+
Sbjct: 181 ---LENSNLVRIGKEIVA 186
HSP 2 Score: 791.2 bits (2042), Expect = 1.7e-225
Identity = 440/739 (59.54%), Postives = 521/739 (70.50%), Query Frame = 1
Query: 388 MDWSSFKDNELDSILQEDDQIQPILKISFNHLPPKLKQCFTYCALFPKDYEFKKNGLVKQ 447
MDW SFKDN+L +ILQ+++QIQPILKISFNHLP LK CFTYCALFPKDYEF+K+GLVKQ
Sbjct: 1 MDWLSFKDNDLGTILQQENQIQPILKISFNHLPSNLKHCFTYCALFPKDYEFQKDGLVKQ 60
Query: 448 WMAQGFIQAHNKKAIEDVGDDYFQELVGRSFFQDIRKNKWGDLKYCKMHDLLHDLACSIG 507
WMAQGFIQ+H+ K IEDVGDDYF+EL+GRSFF +++ NKWGD+K CKMHDL+HDLAC I
Sbjct: 61 WMAQGFIQSHSNKEIEDVGDDYFKELLGRSFFHNVKVNKWGDVKECKMHDLIHDLACWIV 120
Query: 508 ENECVVVSDDVGSIDKRTRHASFLLS-KRLTREVVSKSSIEVTSLRTLDIDSRASFRSFK 567
ENECV SD SIDKRTRH SF + R + E+ +KS EV +LRTL
Sbjct: 121 ENECVDASDKTKSIDKRTRHVSFPSNYSRKSWELEAKSLTEVKNLRTL--------HGPP 180
Query: 568 KTCHMNLFQLRTLNLDRCCCHP-PKFVDKLKHLRYLNLSGLNVTFLPNSITTLYNLETLI 627
N +LR+LNL PKF+ +L+HLRYL++S ++ FLP IT LYNLETLI
Sbjct: 181 FLLSENHLRLRSLNLGYSKFQKIPKFISQLRHLRYLDISDHDMKFLPKFITKLYNLETLI 240
Query: 628 LRYCLWLRKLPKDINNLINLRHLDIYDCSSLTHMPKGLGGMTSLQTMSMFVLGKNKGGDL 687
LR+C LR+LP DINNLINL+HLD++ C LTHMPKGLGG+TSLQTM++FVLGK+KG DL
Sbjct: 241 LRHCSDLRELPTDINNLINLKHLDVHGCYRLTHMPKGLGGLTSLQTMNLFVLGKDKGCDL 300
Query: 688 SALNGLKSLRGLLCIKGLQFCTTADLKN------------LELHWDIKMDHEDALDDGDN 747
S LN L LRG L IKGL+ CTT DLKN L+L W+ + + +N
Sbjct: 301 SELNELARLRGSLLIKGLELCTTTDLKNAKYMEEKFGIQKLKLRWNRDLYDAETDYASEN 360
Query: 748 DDEGVLEGLKPHSNIRKMIIKGYRGMKLCDWFSSNFLGGLVSIELSHCEKLEHLPQFDQF 807
DDE VL+ LKPHSN+ KM I+GYRG+KLC+W S ++LGGLV+IEL CEKL+HLPQFDQF
Sbjct: 361 DDERVLDCLKPHSNVHKMQIRGYRGVKLCNWLSFDYLGGLVNIELQSCEKLQHLPQFDQF 420
Query: 808 LYLKHLLLGYLPNIEYIDSGNSVSSSTTFFPSLEKLRIESMPKLKGWWKGEIS------- 867
+LKHLLL LP+IEYID+ NS+SSS TFFPSLEKL I +MP LKGWWKGE
Sbjct: 421 PFLKHLLLENLPSIEYIDNNNSLSSS-TFFPSLEKLTIMTMPNLKGWWKGETPPESARYS 480
Query: 868 --FPTTILHQLSELCIFYCPLLASIPQHPSLESLRICGVSVQLFQMVIRMATDLSEHSSS 927
FP TILH LS L I CP LASIPQHP L SL + VSVQLF MVI+MAT + + S
Sbjct: 481 ALFP-TILHHLSRLDISNCPQLASIPQHPPLRSLALNDVSVQLFDMVIKMAT--TPAADS 540
Query: 928 SSTLSKLSFLEIGTIDLEFLPVELFCNMTHLESLIIERCKSLQMSSPHPVDEDND-VVWK 987
SS LSKLS L I IDLEFLP ELF + T LE + CK+LQMSS H VDEDND V+ K
Sbjct: 541 SSALSKLSILHIQNIDLEFLPEELFGSTTDLEIFTVVNCKNLQMSSSHLVDEDNDGVLGK 600
Query: 988 KLSNLRTLRLESILKLEYFPKSLKYITSLETLKLSNCENLVSTEGIGELISLSHLEIDRC 1047
KL NL +L + + +LEY K LKY+T+LE L L NC N+VS EGI L SLS L I C
Sbjct: 601 KLGNLHSLGIFDMPQLEYLWKELKYMTTLERLDLYNCPNIVSLEGISHLTSLSSLRICNC 660
Query: 1048 PNLPILSEDVGDLISLSHLLIWNCPKLTSLSEGITRLTSLSSLCLEDCPNLVSLPQEFLH 1103
NL L E + L SLS+L I CP LTSL GI LTSLS+L ++ C NL SLP E +
Sbjct: 661 SNLTSLPEGISHLTSLSYLTIVCCPNLTSLPAGIGHLTSLSTLLIKYCVNLTSLP-EGVS 720
BLAST of Cucsa.128100 vs. TrEMBL
Match:
A0A0A0K5Z6_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_7G425920 PE=4 SV=1)
HSP 1 Score: 103.2 bits (256), Expect = 2.1e-18
Identity = 80/204 (39.22%), Postives = 102/204 (50.00%), Query Frame = 1
Query: 906 STLSKLSFLEIGTIDLEFLPVELFCNMTHLESLIIERCKSLQMSSPHPVDEDNDVVWKKL 965
+TL +L I + +E ++T L SL I C +L S P + L
Sbjct: 615 TTLERLDLYNCPNI----VSLEGISHLTSLSSLRICNCSNLT-SLPEGISH--------L 674
Query: 966 SNLRTLRLESILKLEYFPKSLKYITSLETLKLSNCENLVST-EGIGELISLSHLEIDRCP 1025
++L L + L P + ++TSL TL + C NL S EG+ L SLS I+ CP
Sbjct: 675 TSLSYLTIVCCPNLTSLPAGIGHLTSLSTLLIKYCVNLTSLPEGVSHLTSLSSFTIEECP 734
Query: 1026 NLPILSEDVGDLISLSHLLIWNCPKLTSLSEGITRLTSLSSLCLEDCPNLVSLPQEFLHH 1085
L L E V L SLS L+I C LTSL EGI LTSLS +E+C NL SLP+ L H
Sbjct: 735 CLTSLPEGVSHLTSLSTLIIRRCVNLTSLPEGIGHLTSLSIFTIEECLNLTSLPEGLL-H 794
Query: 1086 HSSLPGGRFLRILNCPKLQIQDKK 1109
SSL G L + CPKL KK
Sbjct: 795 LSSLRGS--LTVSKCPKLSKTWKK 802
HSP 2 Score: 50.4 bits (119), Expect = 1.6e-02
Identity = 34/103 (33.01%), Postives = 57/103 (55.34%), Query Frame = 1
Query: 572 NLFQLRTLNLDRCCCHPPKFVDKLKHLRYLNLSGLN----VTFLPNSITTLYNLETLILR 631
+L L TL L + C + + + HL L+ + +T LP ++ L +L TLI+R
Sbjct: 684 HLTSLSTL-LIKYCVNLTSLPEGVSHLTSLSSFTIEECPCLTSLPEGVSHLTSLSTLIIR 743
Query: 632 YCLWLRKLPKDINNLINLRHLDIYDCSSLTHMPKGLGGMTSLQ 671
C+ L LP+ I +L +L I +C +LT +P+GL ++SL+
Sbjct: 744 RCVNLTSLPEGIGHLTSLSIFTIEECLNLTSLPEGLLHLSSLR 785
HSP 3 Score: 733.4 bits (1892), Expect = 4.1e-208
Identity = 468/1217 (38.46%), Postives = 678/1217 (55.71%), Query Frame = 1
Query: 1 MADSVLFNVAASVITKLGSSALRELGSLWGVNDELDKLQNTLSAIKAVLLDAEEQQSKSH 60
M ++VL ++ ++ KLG+ AL+E+G +WGV DEL+KL+NT S IKA+ LDAEEQQ+KSH
Sbjct: 1 MTEAVLVSITDRILGKLGNLALQEIGLIWGVKDELEKLRNTASVIKAIFLDAEEQQTKSH 60
Query: 61 TVKDWIAKIKDVFYDIDDLIDEFSYETLRRQVLTKDRTITKQVRIFFSKSNQIAFGFKMG 120
V+DW+ K+KD YD DDL+D+FS E L++Q++ +D+ +V FFSK + A+GF M
Sbjct: 61 EVRDWLQKLKDAIYDADDLLDDFSTEMLQQQLMMQDQKAI-EVCAFFSKIKKTAYGFSMS 120
Query: 121 QTIKKVREKLDAIAAIKAQLHLSVCAREVRDNEPRKVRETSSFIPEGEIIGRDEDRKSVM 180
IK +RE+L+ IA+ +++ HL+ R++ + +T SF+ E++GR++D+ +++
Sbjct: 121 CKIKAIRERLNDIASDRSKFHLTDHPRQMPSVIAER-EQTHSFVCVEEVVGREDDKLAIV 180
Query: 181 DFLLNTSNITKDNVEVVSIVGMGGLGKTALAQTVYNDEKINNRFKWKIWVCISQEFDIKV 240
+ LL+++ T++NV V+ +VG+GGLGKT L Q VYN EKI F+ +IWVC+S FD+K+
Sbjct: 181 ELLLHSN--TEENVSVIPVVGIGGLGKTTLVQLVYNSEKIRRHFELRIWVCVSDVFDVKL 240
Query: 241 IVEKILESITKTKQESLQLDILQSMLQEKIYGKKYLLVMDDVWNVDHEKWIGLKRFLMGG 300
IV+KILES T TK + L++D L + L ++I GKK+LL++DDVWN + E+W+ L+ LMGG
Sbjct: 241 IVQKILESATNTKCDGLEMDSLLTRLGKEIDGKKFLLILDDVWNDNRERWLKLRDLLMGG 300
Query: 301 ASGSKILVTTRNLQTAQASDTVWFHHLKELDKDNSWALFRKMAFLNKEEELENSNLVRIG 360
A GSK++VTTR A + T + L+ L +D SW+LF K+AF + ++ EN+ LV IG
Sbjct: 301 ARGSKVVVTTRTQLIATITGTAKPYFLRSLSEDESWSLFEKLAF-KQGKDFENTRLVAIG 360
Query: 361 KEIVAKLKGYPLSIRVVGRLLYFKNTEMDWSSFKDNELDSILQEDDQIQPILKISFNHLP 420
KE+V K G PL+IR +G LLY K+TE +W SFKD +L I Q ++ I PILK+S+ LP
Sbjct: 361 KEVVKKCAGVPLAIRTMGSLLYCKDTETEWLSFKDRDLSMIPQNENDILPILKLSYELLP 420
Query: 421 PKLKQCFTYCALFPKDYEFKKNGLVKQWMAQGFIQ-AHNKKAIEDVGDDYFQELVGRSFF 480
P LK CF YC+LFPKDYE K L+K WMAQGF+Q A + E+VG F +L RSFF
Sbjct: 421 PCLKNCFAYCSLFPKDYEINKQTLIKLWMAQGFLQPADGMQHPEEVGHQCFMDLARRSFF 480
Query: 481 QDIRKNKWGDLKYCKMHDLLHDLACSIGENECVVVSDDVGSIDKRTRHASFLLSKRLTRE 540
QD+ +WGD+ C MHDL+HDLA +G +E V + +I +R RH S L + + +
Sbjct: 481 QDLEYGEWGDVVSCGMHDLMHDLALLVGGSESSTVDSNAENISERIRHVS-LDFELDSSQ 540
Query: 541 VVSKSSIEVTSLRTLDIDSRASFRS-FKKTCHMNLFQ----LRTLNLDRCCCH-PPKFVD 600
+ S + +RT + + +R + H + LR L+ P +
Sbjct: 541 KIPPSLFKANKIRTFVLPVQPVYRKILNQAPHDTIISSFRCLRALDFHNTGVDIVPSSIS 600
Query: 601 KLKHLRYLNLS-GLNVTFLPNSITTLYNLETLILRYCLWLRKLPKDINNLINLRHLDIYD 660
KLKHLRYL+LS ++ LP I L NL+TL L C L LP+ I+ +I+LRHL+I
Sbjct: 601 KLKHLRYLDLSKNEDLKRLPRCIARLKNLQTLKLSSCKRLEALPRHISKMISLRHLEIDQ 660
Query: 661 CSSLTHMPKGLGGMTSLQTMSMFVLGK-NKGGDLSA----LNGLKSLRGLLCIKGLQFCT 720
C+ LTHMP GLG +T+LQT++ FV+GK DLSA LNGL LRG I L+
Sbjct: 661 CTGLTHMPNGLGQLTALQTLTQFVVGKYGSSPDLSARLRELNGLNDLRGEFKISKLEKLK 720
Query: 721 TA-------------DLKNLELHWDIKMDHEDALDDGDNDDEGVLEGLKPHSNIRKMIIK 780
+ +L+ L L W ++ + +D +DE +LE +PHSN+++ I
Sbjct: 721 VSATESREANLKGKENLEVLRLEWTRGVNDDRVID----EDEVLLESFQPHSNLKEFHIY 780
Query: 781 GYRGMKLCDWFSSN---FLGGLVSIELSHCEKLEHLPQFDQFLYLKHLLLGYLPNIEYID 840
GYR K W N L L I + C + LP F Q LK L L + +EYI+
Sbjct: 781 GYRAGKFPSWMVLNLSLLLPNLQEIIIWRCYRCLELPMFSQLPMLKVLKLEEVTALEYIE 840
Query: 841 SGNSVSS--------STTFFPSLEKLRIESMPKLKGWWKGE------------------- 900
+ ++ SS S FFPSL++LR+ + KGWW+ E
Sbjct: 841 NSSNGSSSLGKREEKSALFFPSLQELRLFDLRNFKGWWREEVSVVNNDEATVETTTETAG 900
Query: 901 ISFPTTI-------LHQ---------LSELCIFYCPLLASIPQHPSLESLRICGVSVQLF 960
IS P+ L Q LS+L I +CP L+++P HP L + V+ L
Sbjct: 901 ISLPSVAACEKQQPLQQQLVLPSFPCLSKLTIGHCPNLSNLPLHPFLNEVEFKDVNAGLV 960
Query: 961 QMVIRMATDLSEHSSSSSTLSKLSF---LEI------GTIDLEFLPVELFCNMTHLESLI 1020
Q + + S+S S SF L++ +DL + N+T+LE L
Sbjct: 961 QWSMVGLASIEGSSASGRNTSLPSFPSTLKLKHLCMDSVMDLVSISELGLQNLTYLEHLT 1020
Query: 1021 IERCKSLQMSSPHPVDEDNDVVWKKLSNLRTLRLESIL---KLEYFPKSLKYITSLETLK 1080
IE C +L S P + L LR+LR SI L L+Y+TSLE L+
Sbjct: 1021 IENCPNLS-SLPE----------ESLRGLRSLRFLSIRGCGSLTSLFLGLQYLTSLEELE 1080
Query: 1081 LSNCENLVSTEGIGE-------LISLSHLEIDRCPNLPILSEDVGDLISLSHLLIWNCPK 1127
+ C L ++ E + SL L+I P L + + + ++ SL L I C
Sbjct: 1081 IEECRALDMSDCDEENSLQFRGMKSLRRLKIGYMPQLESIPDGIHEVTSLQDLKIEGCVG 1140
BLAST of Cucsa.128100 vs. TrEMBL
Match:
A0A0D2NDD9_GOSRA (Uncharacterized protein OS=Gossypium raimondii GN=B456_005G155200 PE=3 SV=1)
HSP 1 Score: 729.2 bits (1881), Expect = 7.8e-207
Identity = 455/1177 (38.66%), Postives = 659/1177 (55.99%), Query Frame = 1
Query: 1 MADSVLFNVAASVITKLGSSALRELGSLWGVNDELDKLQNTLSAIKAVLLDAEEQQSKSH 60
MA++ LFN+A ++ K+G+ AL+E+G +WGV ++L+KL+NT+S IKAVLLDAE+Q +KSH
Sbjct: 1 MAEAGLFNIADGILGKIGNLALQEIGLVWGVKEQLEKLKNTVSTIKAVLLDAEDQHAKSH 60
Query: 61 TVKDWIAKIKDVFYDIDDLIDEFSYETLRRQVLTKDRTITKQVRIFFSKSNQIAFGFKMG 120
V+DW+ K+KD YD DDL+D FS L+RQ + KQV FSK +Q+ + K+
Sbjct: 61 EVRDWLGKLKDAVYDTDDLLDNFSTHVLKRQQGKRG----KQVSFLFSKVSQVTYNLKIS 120
Query: 121 QTIKKVREKLDAIAAIKAQLHLSVCAREVRDNEPRKV-------RETSSFIPEGEIIGRD 180
I+ +REKLDAIAA K + H + D P + R+T SF+ + +++GR
Sbjct: 121 HQIEAIREKLDAIAADKIKYHFT-------DRSPISIPLVKVERRQTHSFVRKEDVVGRQ 180
Query: 181 EDRKSVMDFLLNTSNITKDNVEVVSIVGMGGLGKTALAQTVYNDEKINNRFKWKIWVCIS 240
D+ ++M LL ++ + DNV V+ IVG+GG GKT LAQ YNDE+ F+ ++WVC+S
Sbjct: 181 GDKDAIMKRLLESNGV--DNVSVIPIVGIGGQGKTTLAQLAYNDERTVKHFELRMWVCVS 240
Query: 241 QEFDIKVIVEKILESITKTKQESLQLDILQSMLQEKIYGKKYLLVMDDVWNVDHEKWIGL 300
FD+K+IVEKILES T +K ESL++D LQ+ L+++I G+KYLLV+DD+WN E+W+ L
Sbjct: 241 DVFDVKMIVEKILESATSSKYESLEMDSLQTHLRKRIDGRKYLLVLDDMWNDSRERWLNL 300
Query: 301 KRFLMGGASGSKILVTTRNLQTAQASDTVWFHHLKELDKDNSWALFRKMAFLNKEEELEN 360
LM GA GSKI+VTTR A + T + L+ L +D SW+LF K+AF + +E +
Sbjct: 301 ADLLMNGARGSKIIVTTRAQLVASITGTSQPYLLEGLPEDMSWSLFEKVAF-KESKETND 360
Query: 361 SNLVRIGKEIVAKLKGYPLSIRVVGRLLYFKNTEMDWSSFKDNELDSILQEDDQIQPILK 420
S LV IGK+IV K G PL IR +G +LY K+TE +W S K+ +L + + +D + +LK
Sbjct: 361 SRLVAIGKDIVKKCAGNPLVIRTIGGVLYTKDTETEWLSLKERQLSMLTRNEDDVLSVLK 420
Query: 421 ISFNHLPPKLKQCFTYCALFPKDYEFKKNGLVKQWMAQGFIQ-AHNKKAIEDVGDDYFQE 480
+S+ LP LKQCF YC+LFPKDYE K L+ W+A+GFIQ + +E++G YF +
Sbjct: 421 LSYEQLPSYLKQCFAYCSLFPKDYEINKQMLISLWIAEGFIQPLQGIQCLEELGGQYFMD 480
Query: 481 LVGRSFFQDIRKNKWGDLKYCKMHDLLHDLACSIGENECVVVSDDVGSIDKRTRHASFLL 540
L+ RSFFQD+ ++WG++ CK+HDL+HDLA I ++C +V D +I +RT H S L
Sbjct: 481 LLRRSFFQDVEYDEWGNVISCKLHDLMHDLAQLIAGSDCSMVDLDCENISERTHHVS--L 540
Query: 541 SKRLTRE-VVSKSSIEVTSLRTLDIDSRASFR-SFKKTCHMNLFQ----LRTLNLDRCCC 600
S L + + + +RT + + R K H + +R L+L
Sbjct: 541 SAELDSSWKIPTTLLNANKIRTFLLPMQPIHRVVLDKVDHEAIISSFRLMRVLDLHNTGL 600
Query: 601 H-PPKFVDKLKHLRYLNLSGLNV-TFLPNSITTLYNLETLILRYCLWLRKLPKDINNLIN 660
H P+ + KLKHLRYL+LS V LP+SIT L NL+TL + C L +LP+ ++N+I+
Sbjct: 601 HILPRIIGKLKHLRYLDLSKNEVIRKLPSSITELLNLQTLKIYSCKRLEQLPRKLSNMIS 660
Query: 661 LRHLDIYDCSSLTHMPKGLGGMTSLQTMSMFVLGKNK----GGDLSALNGLKSLRGLLCI 720
L+HL+ C+ LTHMP G+G +TSLQT++ FV+G + G L L L LRG L I
Sbjct: 661 LKHLETGQCTGLTHMPSGIGQLTSLQTLTRFVVGMSSFEMASGGLRELKDLNELRGELMI 720
Query: 721 KGLQ-------FCTTADLKN------LELHWDIKMDHEDALDDGDNDDEGVLEGLKPHSN 780
L+ C A+LK L L W ++++ + + +DE +LEGL+PHSN
Sbjct: 721 AKLENLRNVAAECEEANLKEKQHLEVLTLDWSREVNNHVSFE----EDEALLEGLQPHSN 780
Query: 781 IRKMIIKGYRGMKLCDWFSSN---FLGGLVSIELSHCEKLEHLPQFDQFLYLKHLLLGYL 840
+++ I GYR + W S + L L+ I + +C K HLP F Q LK L L +
Sbjct: 781 LQEFHIYGYRAERFPKWMSFDMALLLPNLLEITIWNCIKCIHLPLFSQLPKLKVLRLEVI 840
Query: 841 PNIEYIDSGNSVSSST---------------TFFPSLEKLRIESMPKLKGWWK------- 900
+EYI+ + SSS+ FFP LE+L + LKGWW+
Sbjct: 841 TAVEYIEDSRAESSSSLSFKGNPMNRGREGKEFFPCLEELVFFDLRNLKGWWREAPPVTN 900
Query: 901 ---GEISFPTTILHQ---------LSELCIFYCPLLASIPQHPSLESLRICGVSVQLFQM 960
G + P L + LS+L I C L +P HP LE L + +L Q
Sbjct: 901 NNHGAAASPQRPLQKKESMVSFPCLSKLKIGICTNLTHMPLHPFLEELELKNTPARLLQQ 960
Query: 961 VIRMATDLSEHSSSSSTLSKLSFLEI-GTIDLEFLPVELFCNMTHLESLIIERCKSLQMS 1020
+A + LSKL + I G IDL P + ++T L+ L IE C L
Sbjct: 961 SAMVAA--GANLVYPLYLSKLKVMHIDGIIDLVSFPEKGIHHLTSLQHLSIENCPDLVCL 1020
Query: 1021 SPHPVDEDNDVVWKKLSNLRTLRLESILKLEYFPKSLKYITSLETLKLSNCENLVST--- 1080
+ + K L++LR + L+ K K++T+LE L++ C L +
Sbjct: 1021 TEEGL--------KSLTSLRFFNIRCCEILKSLFKGFKHLTALEELEIKECRELDLSKDL 1080
Query: 1081 -EGIGELISLSHLEIDRCPNLPILSEDVGDLISLSHLLIWNCPKLTSLSEGITRLTSLSS 1103
E + EL L L I+ P L L + + + +L L I +C L +L E I LTSL
Sbjct: 1081 EENVMELQCLRTLRIEDMPKLSSLPDGLQHVTTLKDLQISSCSNLKTLPEWIGNLTSLQR 1140
BLAST of Cucsa.128100 vs. TrEMBL
Match:
A0A0A0KIT2_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_6G375730 PE=3 SV=1)
HSP 1 Score: 726.1 bits (1873), Expect = 6.6e-206
Identity = 412/759 (54.28%), Postives = 524/759 (69.04%), Query Frame = 1
Query: 362 EIVAKLKGYPLSIRVVGRLLYFKNTEMDWSSFKDNELDSILQEDDQIQPILKISFNHLPP 421
EIV KLKG PL+IRV+G LY K +E DW SFKD+ELD+I+Q++++IQ ILKISFNHL
Sbjct: 196 EIVTKLKGSPLAIRVIGSYLYSKKSEKDWLSFKDHELDTIMQQENEIQSILKISFNHLSS 255
Query: 422 KLKQCFTYCALFPKDY--EFKKNGLVKQWMAQGFIQAHNKKAIEDVGDDYFQELVGRSFF 481
LK CFTYCALF KDY E +KN L+KQWMAQGFIQ HNKKA+EDVGDDYF+EL+GRSFF
Sbjct: 256 SLKHCFTYCALFSKDYHHEIRKNDLIKQWMAQGFIQPHNKKAMEDVGDDYFEELLGRSFF 315
Query: 482 QDIRKNKWGDLKYCKMHDLLHDLACSIGENECVVVSDDVGSIDKRTRHASFLLSKRLTR- 541
QDIRKNKWG++ KMHD++HDLACS+ EN+CV+ +DD SIDKRTRH S +TR
Sbjct: 316 QDIRKNKWGEINKFKMHDIIHDLACSVVENDCVLANDDTKSIDKRTRHVSISAFNSMTRW 375
Query: 542 EVVSKSSIEVTSLRTLDIDSRASFRSFKKTCHMNL---FQLRTLNLDRCCCHPPKFVDKL 601
++++KS IE +LRTL ++ + H++L +LRTLNL+ PK + K+
Sbjct: 376 KLITKSLIEAKNLRTL---------NYARRHHIDLSNHLRLRTLNLE--FHFVPKCIGKM 435
Query: 602 KHLRYLNLSGLNVTFLPNSITTLYNLETLILRYCLWLRKLPKDINNLINLRHLDIYDCSS 661
KHLRY+N++ + FLP ++T LY+LETLI+R CL LR+L DI NLINLRHLDI D
Sbjct: 436 KHLRYINITYCYIDFLPKAVTKLYHLETLIIRGCLELRELSSDIKNLINLRHLDIKDFKH 495
Query: 662 L-THMPKGLGGMTSLQTMSMFVLGKNKGGDLSALNGLKSLRGLLCIKGLQFCTTADLKN- 721
+ ++MPKG+G MT+LQTM++F+LG+NKGG+LS LNGL +LRG L I+ LQFC L+N
Sbjct: 496 VWSYMPKGMGSMTTLQTMNLFILGENKGGELSELNGLVNLRGSLSIQQLQFCKPIGLENV 555
Query: 722 -----------LELHWDIKMDHEDALDDGDNDDEGVLEGLKPHSNIRKMIIKGYRGMKLC 781
LELHW E +DD +DE VLE LKPHSN++K+ I+GYRG+KLC
Sbjct: 556 KYLEEKSRIQKLELHWK-TYQRESKIDD---EDERVLESLKPHSNLQKIRIEGYRGLKLC 615
Query: 782 DWFSSNFLGGLVSIELSHCEKLEHLPQFDQFLYLKHLLLGYLPNIEYIDSGNSVSSS-TT 841
+WFS + + LV I+L +CEKL+ LP+FD+F +LKHL L LP+IEYI N VSSS TT
Sbjct: 616 NWFSFDSIVNLVFIKLFNCEKLQQLPRFDRFPFLKHLHLEDLPSIEYIAINNYVSSSMTT 675
Query: 842 FFPSLEKLRIESMPKLKGWWKGE-----ISFPTTILHQLSELCIFYCPLLASIPQHPSLE 901
FFPSLE L I +P LK WWKGE SFP TIL LS+L I YC LASIPQH L+
Sbjct: 676 FFPSLENLSIIKLPNLKEWWKGESIDQNTSFP-TILRHLSQLKIHYCRQLASIPQHGPLQ 735
Query: 902 SLRICGVSVQLFQMVIRM-ATDL--SEHSSSSST---LSKLSFLEIGTIDLEFLPVELFC 961
SL I +S+QLF++VI+M AT++ E SSSS+ + S L+I ID EFLP +LF
Sbjct: 736 SLDIRDISLQLFELVIKMTATNIIVGEDSSSSANDMFIRSSSSLKIWKIDWEFLPNDLFS 795
Query: 962 NMTHLESLIIERCKSLQMSSPHPVDEDNDVVWKKLSNLRTLRLESILKLEYFPKSLKYIT 1021
N+THL+SL+I RC +L+MS +D++V WK+L +LRTLRL I KLEY PK +Y+
Sbjct: 796 NVTHLQSLVIGRCFNLKMSF-----DDDNVRWKELGSLRTLRLCFIPKLEYLPKGFQYLK 855
Query: 1022 SLETLKLSNCENLVSTEGIGELISLSHLEIDRCPNLPILSEDVGDLISLSHLLIWNCPKL 1081
+LE L+L CENL GI L SLS LEI NCP L
Sbjct: 856 ALEHLELLWCENLACILGIEHLTSLSRLEIS------------------------NCPNL 908
Query: 1082 TSLSEGITRLTSLSSLCLEDCPNLVSLPQEFLHHHSSLP 1090
TSL EG+T+L SL+ L ++DCPNL +LP E LHH + P
Sbjct: 916 TSLPEGMTQLISLTCLIIDDCPNLSTLP-EGLHHLLNTP 908
BLAST of Cucsa.128100 vs. TAIR10
Match:
AT3G14470.1 (AT3G14470.1 NB-ARC domain-containing disease resistance protein)
HSP 1 Score: 431.0 bits (1107), Expect = 2.2e-120
Identity = 315/1011 (31.16%), Postives = 515/1011 (50.94%), Query Frame = 1
Query: 35 LDKLQNTLSAIKAVLLDAEEQQSKSHTVKDWIAKIKDVFYDIDDLIDEFSYETLRRQVLT 94
L++L L I AVL+DAEE+Q + V+ W+ +++DV Y +D +D+ + E LR +
Sbjct: 39 LERLSTALLTITAVLIDAEEKQITNPVVEKWVNELRDVVYHAEDALDDIATEALRLNIGA 98
Query: 95 KDRTITK--QVRIFFSKSNQIAFGFKMGQT-IKKVREKLDAIAAIKAQLHLSVCAREVRD 154
+ + + Q+R S + + + +T ++KV +L+ +A+ + L L +E+
Sbjct: 99 ESSSSNRLRQLRGRMSLGDFLDGNSEHLETRLEKVTIRLERLASQRNILGL----KELTA 158
Query: 155 NEPRKVRETSSFIPEGEIIGRDEDRKSVMDFLLNTSNITKDN-VEVVSIVGMGGLGKTAL 214
P++ T+S + E E+ GRD+D+ +M FL+ + KDN + VV+IVG+GG+GKT L
Sbjct: 159 MIPKQRLPTTSLVDESEVFGRDDDKDEIMRFLIPENG--KDNGITVVAIVGIGGVGKTTL 218
Query: 215 AQTVYNDEKINNRFKWKIWVCISQEFDIKVIVEKILESITKTKQESLQLDILQSMLQEKI 274
+Q +YND+ + + F K+W +S+EFD+ I +K+ ES+T E LD+LQ L+E++
Sbjct: 219 SQLLYNDQHVRSYFGTKVWAHVSEEFDVFKITKKVYESVTSRPCEFTDLDVLQVKLKERL 278
Query: 275 YGK--KYLLVMDDVWNVDHEKWIGLKRFLMGGASGSKILVTTRNLQTAQASDTVWFHHLK 334
G +LLV+DD+WN + W L++ + A GS+ILVTTR+ + A V H+L+
Sbjct: 279 TGTGLPFLLVLDDLWNENFADWDLLRQPFIHAAQGSQILVTTRSQRVASIMCAVHVHNLQ 338
Query: 335 ELDKDNSWALFRKMAFLNKEEELENSNLVRIGKEIVAKLKGYPLSIRVVGRLLYFKNTEM 394
L + W+LF K F N+E L N + + + IV K +G PL+++ +G +L F+ +
Sbjct: 339 PLSDGDCWSLFMKTVFGNQEPCL-NREIGDLAERIVHKCRGLPLAVKTLGGVLRFEGKVI 398
Query: 395 DWSSFKDNELDSILQEDDQIQPILKISFNHLPPKLKQCFTYCALFPKDYEFKKNGLVKQW 454
+W + + + + + P+L++S+ +LP LK+CF YC++FPK + F+K+ +V W
Sbjct: 399 EWERVLSSRIWDLPADKSNLLPVLRVSYYYLPAHLKRCFAYCSIFPKGHAFEKDKVVLLW 458
Query: 455 MAQGFI-QAHNKKAIEDVGDDYFQELVGRSFFQDIRKNKWGDLKYCKMHDLLHDLA-CSI 514
MA+GF+ Q + K +E++G++YF EL RS Q + +Y MHD +++LA +
Sbjct: 459 MAEGFLQQTRSSKNLEELGNEYFSELESRSLLQKTK------TRYI-MHDFINELAQFAS 518
Query: 515 GENECVVVSDDVGSIDKRTRHASFLLSKRLTREVVSKSSIEVTSLRT-LDIDSRASFRS- 574
GE + +RTR+ S+ L + ++ EV LRT L + S RS
Sbjct: 519 GEFSSKFEDGCKLQVSERTRYLSY-LRDNYAEPMEFEALREVKFLRTFLPLSLTNSSRSC 578
Query: 575 -----FKKTCHMNLFQLRTLNLD--RCCCHPPKFVDKLKHLRYLNLSGLNVTFLPNSITT 634
+ L +LR L+L + PP F + H R+L+LS + LP S+
Sbjct: 579 CLDQMVSEKLLPTLTRLRVLSLSHYKIARLPPDFFKNISHARFLDLSRTELEKLPKSLCY 638
Query: 635 LYNLETLILRYCLWLRKLPKDINNLINLRHLDIYDCSSLTHMPKGLGGMTSLQTMSMFVL 694
+YNL+TL+L YC L++LP DI+NLINLR+LD+ + L MP+ G + SLQT++ F +
Sbjct: 639 MYNLQTLLLSYCSSLKELPTDISNLINLRYLDLIG-TKLRQMPRRFGRLKSLQTLTTFFV 698
Query: 695 GKNKGGDLSALNGLKSLRGLLCIKGLQ-FCTTAD-----------LKNLELHW--DIKMD 754
+ G +S L GL L G L I LQ AD L+ ++ W
Sbjct: 699 SASDGSRISELGGLHDLHGKLKIVELQRVVDVADAAEANLNSKKHLREIDFVWRTGSSSS 758
Query: 755 HEDALDDGDNDDEGVLEGLKPHSNIRKMIIKGYRGMKLCDWFSSNFLGGLVSIELSHCEK 814
+ ++ V E L+PH +I K+ I+ Y+G + DW S +V I L C+
Sbjct: 759 ENNTNPHRTQNEAEVFEKLRPHRHIEKLAIERYKGRRFPDWLSDPSFSRIVCIRLRECQY 818
Query: 815 LEHLPQFDQFLYLKHLLLGYLPNIE------YIDSGNSVSSSTTFFPSLEKLRIESMPKL 874
LP Q LK L + + ++ Y F SLE LR +++P
Sbjct: 819 CTSLPSLGQLPCLKELHISGMVGLQSIGRKFYFSDQQLRDQDQQPFRSLETLRFDNLPDW 878
Query: 875 KGWWKGEISFPTTILHQLSELCIFYCP-LLASIPQH-PSLESLRICGVSVQLFQMVIRMA 934
+ W ++ + L +L I CP L ++P PSL SL I + FQ
Sbjct: 879 QEWLDVRVT-RGDLFPSLKKLFILRCPELTGTLPTFLPSLISLHIYKCGLLDFQP----- 938
Query: 935 TDLSEHSSSSSTLSKLSFLEIGTIDLEFLPVELFCNMTHLESLIIERCK---SLQMSSPH 994
H S L LS ++ L P+ F N+ LE +++C SL++S+ H
Sbjct: 939 ---DHHEYSYRNLQTLS-IKSSCDTLVKFPLNHFANLDKLE---VDQCTSLYSLELSNEH 998
Query: 995 PVDEDNDVVWKKLSNLRTLRLESILKLEYFPKSLKYITSLETLKLSNCENL 1004
+ + LR LR+ L+ PK L + + ++NC L
Sbjct: 999 ---------LRGPNALRNLRINDCQNLQLLPK-LNALPQNLQVTITNCRYL 1010
BLAST of Cucsa.128100 vs. TAIR10
Match:
AT3G14460.1 (AT3G14460.1 LRR and NB-ARC domains-containing disease resistance protein)
HSP 1 Score: 369.0 bits (946), Expect = 1.0e-101
Identity = 278/903 (30.79%), Postives = 435/903 (48.17%), Query Frame = 1
Query: 1 MADSVLFNVAASVITKLGSSA-LRELGSLWGVNDELDKLQNTLSAIKAVLLDAEEQQSKS 60
MA+S L + A ++ ++ +S L EL + L +L+ L VL DA+++
Sbjct: 1 MANSYLSSCANVMVERINTSQELVELCKGKSSSALLKRLKVALVTANPVLADADQRAEHV 60
Query: 61 HTVKDWIAKIKDVFYDIDDLIDEFSYETLRRQVLTKDRTITKQVRIFFSKSNQIAFGFKM 120
VK W+ IKD F+ +D++DE E LRR+V+ + + + + I
Sbjct: 61 REVKHWLTGIKDAFFQAEDILDELQTEALRRRVVAEAGGLGGLFQNLMAGREAIQ----- 120
Query: 121 GQTIKKVREKLDAIAAIKAQ--LHLSVCA----REVRDNEPRKV-RETSSFIPEGEIIGR 180
KK+ K++ + + H+ V E R+ + R+ R +P+G ++GR
Sbjct: 121 ----KKIEPKMEKVVRLLEHHVKHIEVIGLKEYSETREPQWRQASRSRPDDLPQGRLVGR 180
Query: 181 DEDRKSVMDFLLNTSNITKDNVEVVSIVGMGGLGKTALAQTVYNDEKINNRFKWKIWVCI 240
ED+ ++++ LL+ I+ V+S+VGM G+GKT L + V+ND ++ F+ K+W+
Sbjct: 181 VEDKLALVNLLLSDDEISIGKPAVISVVGMPGVGKTTLTEIVFNDYRVTEHFEVKMWISA 240
Query: 241 SQEFDIKVIVEKILESITKTKQESLQLDILQSMLQEKIYGKKYLLVMDDVWNVDHEKWIG 300
F++ + + +L+ IT + + L LQ L++ + GK++LLV+DD W+ +W
Sbjct: 241 GINFNVFTVTKAVLQDITSSAVNTEDLPSLQIQLKKTLSGKRFLLVLDDFWSESDSEWES 300
Query: 301 LKRFLMGGASGSKILVTTRNLQTAQASDTVWFHHLKELDKDNSWALFRKMAFLNKEEELE 360
+ GSKI++TTR+ + + + +K + + W L + AF N
Sbjct: 301 FQVAFTDAEEGSKIVLTTRSEIVSTVAKAEKIYQMKLMTNEECWELISRFAFGNISVGSI 360
Query: 361 NSNLVRIGKEIVAKLKGYPLSIRVVGRLLYFKNTEMDWSSFKDNELDSILQEDDQIQPIL 420
N L IGK I + KG PL+ R + L K DW + N + I P+L
Sbjct: 361 NQELEGIGKRIAEQCKGLPLAARAIASHLRSKPNPDDWYAVSKN----FSSYTNSILPVL 420
Query: 421 KISFNHLPPKLKQCFTYCALFPKDYEFKKNGLVKQWMAQGFI-QAHNKKAIEDVGDDYFQ 480
K+S++ LPP+LK+CF C++FPK + F + LV WMA + Q + + +ED+G+DY
Sbjct: 421 KLSYDSLPPQLKRCFALCSIFPKGHVFDREELVLLWMAIDLLYQPRSSRRLEDIGNDYLG 480
Query: 481 ELVGRSFFQ--DIRKNKWGDLKYCKMHDLLHDLACSIGENECVVVSDD-VGSIDKRTRHA 540
+LV +SFFQ DI + MHDL++DLA ++ + C + DD + I TRH
Sbjct: 481 DLVAQSFFQRLDITMTSF------VMHDLMNDLAKAVSGDFCFRLEDDNIPEIPSTTRHF 540
Query: 541 SFLLSKRLTREVVSKSSIEVTSLRT-LDIDSRASFRSFKKTCHM------NLFQLRTLNL 600
SF S + V +S LRT L +S S S + T + L LR L+L
Sbjct: 541 SFSRS-QCDASVAFRSICGAEFLRTILPFNSPTSLESLQLTEKVLNPLLNALSGLRILSL 600
Query: 601 DRC-CCHPPKFVDKLKHLRYLNLSGLNVTFLPNSITTLYNLETLILRYCLWLRKLPKDIN 660
+ PK + LK LRYL+LS + LP + TL NL+TL+L C L LPK I
Sbjct: 601 SHYQITNLPKSLKGLKLLRYLDLSSTKIKELPEFVCTLCNLQTLLLSNCRDLTSLPKSIA 660
Query: 661 NLINLRHLDIYDCSSLTHMPKGLGGMTSLQTMSMFVLGKNKGGDLSALNGLKSLRGLLCI 720
LINLR LD+ + L MP G+ + SLQ +S FV+G+ G L L L LRG L I
Sbjct: 661 ELINLRLLDLVG-TPLVEMPPGIKKLRSLQKLSNFVIGRLSGAGLHELKELSHLRGTLRI 720
Query: 721 KGLQ---FCTTAD---------LKNLELHWDIKMDH--EDALDDGDNDDEGVLEGLKPHS 780
LQ F + A L L L W +K + + D + VL L+PH
Sbjct: 721 SELQNVAFASEAKDAGLKRKPFLDGLILKWTVKGSGFVPGSFNALACDQKEVLRMLEPHP 780
Query: 781 NIRKMIIKGYRGMKLCDWFSSNFLGGLVSIELSHCEKLEHLPQFDQFLYLKHLLLGYLPN 840
+++ I+ Y+G W + G+ S+ LS C LP Q LK+L +
Sbjct: 781 HLKTFCIESYQGGAFPKWLGDSSFFGITSVTLSSCNLCISLPPVGQLPSLKYLSIEKFNI 840
Query: 841 IEYIDSG---NSVSSSTTFFPSLEKLRIESMPKLKGWWKGEISFPTTILHQLSELCIFYC 867
++ + +S F SL+ L+ MP+ W E+ I L +L I C
Sbjct: 841 LQKVGLDFFFGENNSRGVPFQSLQILKFYGMPRWDEWICPELE--DGIFPCLQKLIIQRC 880
HSP 2 Score: 62.4 bits (150), Expect = 2.1e-09
Identity = 90/330 (27.27%), Postives = 131/330 (39.70%), Query Frame = 1
Query: 778 LSHCEKLEHLPQFDQFLYLKHLLLGYLPN-------IEYIDSGNSVSSSTTFFPSLEKLR 837
LS K FDQ+ LG LP I SG +T P + +
Sbjct: 970 LSSLPKDRQTEDFDQY----ETQLGSLPQQFEEPAVISARYSGYISDIPSTLSPYMSRTS 1029
Query: 838 IESMPKLKGWWKGEISFPTTILHQLSELCIFYCPLLASIPQHPSLESLRICGVSVQLFQM 897
+ PK +G +IL S + +S+P S E+++ M
Sbjct: 1030 LVPDPKNEG----------SILPGSSSYQYHQYGIKSSVPSPRSSEAIKPSQYDDDETDM 1089
Query: 898 VIRMATDLSEHSSSSSTLSKLSFLEIGTID-LEFLPVELFCNMTHLESLIIERCKSLQ-M 957
TD+S L L I + D L LP L + +L L+I C SL+
Sbjct: 1090 EYLKVTDISHLMELPQNLQSL---HIDSCDGLTSLPENLTESYPNLHELLIIACHSLESF 1149
Query: 958 SSPHPVDEDNDVV---WKKLSNLRTLR-LESILKLEY------------FPKSLKYITSL 1017
HP + KKL+ +L+ S +LEY FP SL L
Sbjct: 1150 PGSHPPTTLKTLYIRDCKKLNFTESLQPTRSYSQLEYLFIGSSCSNLVNFPLSL--FPKL 1209
Query: 1018 ETLKLSNCENLVSTE---GIGE-LISLSHLEIDRCPNLPILSEDVGDLISLSHLLIWNCP 1077
+L + +CE+ + G+G+ I+L LEI CPNL + LS +L+ NC
Sbjct: 1210 RSLSIRDCESFKTFSIHAGLGDDRIALESLEIRDCPNLETFPQGGLPTPKLSSMLLSNCK 1269
Query: 1078 KLTSLSEGITRLTSLSSLCLEDCPNLVSLP 1079
KL +L E + LTSL SL + CP + ++P
Sbjct: 1270 KLQALPEKLFGLTSLLSLFIIKCPEIETIP 1280
HSP 3 Score: 35.0 bits (79), Expect = 3.6e-01
Identity = 30/85 (35.29%), Postives = 47/85 (55.29%), Query Frame = 1
Query: 963 KKLSNLRTLRLESILKLEYFPKSLKYITSLETLKLSNCENLVS-TEGIGELISLSHLEID 1022
K L LR L L S K++ P+ + + +L+TL LSNC +L S + I ELI+L L++
Sbjct: 593 KGLKLLRYLDLSST-KIKELPEFVCTLCNLQTLLLSNCRDLTSLPKSIAELINLRLLDLV 652
Query: 1023 RCP--NLPILSEDVGDLISLSHLLI 1045
P +P + + L LS+ +I
Sbjct: 653 GTPLVEMPPGIKKLRSLQKLSNFVI 676
BLAST of Cucsa.128100 vs. TAIR10
Match:
AT1G58602.1 (AT1G58602.1 LRR and NB-ARC domains-containing disease resistance protein)
HSP 1 Score: 265.4 bits (677), Expect = 1.6e-70
Identity = 304/1183 (25.70%), Postives = 520/1183 (43.96%), Query Frame = 1
Query: 14 ITKLGSSALRELGSLWGVNDELDKLQNTLSAIKAVLLDAEEQQSKSHTVKDWIAKIKDVF 73
+ KL E GV D++ +L++ L+ +K+ L DA+ ++ S V+ + +IKD+
Sbjct: 10 VNKLWDLLSHEYTLFQGVEDQVAELKSDLNLLKSFLKDADAKKHTSALVRYCVEEIKDIV 69
Query: 74 YDIDDLIDEFSYETLRRQVLTKDRTITKQV-RIFFSKSNQIAFGFKMGQTIKKVREKLDA 133
YD +D+++ F ++++ L I K + R+ ++ +G K++ +
Sbjct: 70 YDAEDVLETF----VQKEKLGTTSGIRKHIKRLTCIVPDRREIALYIGHVSKRITRVIRD 129
Query: 134 IAAIKAQLHLSVCAREVRDNEPRKVRETSSFIPEGEIIGRDEDRKSVMDFLLNTSNITKD 193
+ + Q + N R++R T E + +E+ K ++ + + +D
Sbjct: 130 MQSFGVQQMIVDDYMHPLRNREREIRRTFPKDNESGFVALEENVKKLVGYF-----VEED 189
Query: 194 NVEVVSIVGMGGLGKTALAQTVYNDEKINNRFKWKIWVCISQEFDIKVIVEKILESITKT 253
N +VVSI GMGGLGKT LA+ V+N + + +F WV +SQ+F +K + + IL +
Sbjct: 190 NYQVVSITGMGGLGKTTLARQVFNHDMVTKKFDKLAWVSVSQDFTLKNVWQNILGDLKPK 249
Query: 254 KQESLQ-----LDILQSMLQEKIYG----KKYLLVMDDVWNVDHEKWIGLKRFLMGGASG 313
++E+ + L++ + LQ ++Y K L+V+DD+W E W +K + G
Sbjct: 250 EEETKEEEKKILEMTEYTLQRELYQLLEMSKSLIVLDDIWK--KEDWEVIKP-IFPPTKG 309
Query: 314 SKILVTTRNLQTAQASDTVWFHHLKE-LDKDNSWALFRKMAF-LNKEEELE-NSNLVRIG 373
K+L+T+RN ++T +F+ E L D+SW LF+++AF +N E E + + ++G
Sbjct: 310 WKLLLTSRNESIVAPTNTKYFNFKPECLKTDDSWKLFQRIAFPINDASEFEIDEEMEKLG 369
Query: 374 KEIVAKLKGYPLSIRVVGRLLYFKNTEMDWSSFKDNELDSIL--------QEDDQIQPIL 433
++++ G PL+I+V+G +L K T DW +N ++ ++ +L
Sbjct: 370 EKMIEHCGGLPLAIKVLGGMLAEKYTSHDWRRLSENIGSHLVGGRTNFNDDNNNSCNYVL 429
Query: 434 KISFNHLPPKLKQCFTYCALFPKDYEFKKNGLVKQWMAQGFIQA--HNKKAIEDVGDDYF 493
+SF LP LK CF Y A FP+DYE K L W A+ Q ++ + I DVGD Y
Sbjct: 430 SLSFEELPSYLKHCFLYLAHFPEDYEIKVENLSYYWAAEEIFQPRHYDGEIIRDVGDVYI 489
Query: 494 QELVGRSFFQDIRKNKWGDLKYCKMHDLLHD--LACSIGENECVVVSDDVGSIDKRTRHA 553
+ELV R+ R K + C +HD++ + L + EN + S+ + + ++
Sbjct: 490 EELVRRNMVISERDVKTSRFETCHLHDMMREVCLLKAKEENFLQITSNPPSTANFQSTVT 549
Query: 554 SFLLSKRLTREVVSKSSIEVTSLRTLDIDSRASFRSFKKTCHMNLFQLRTLNLDRCCCHP 613
S L + + + I LR+L + + S+ + + L LR L+L +
Sbjct: 550 SRRLVYQYPTTLHVEKDINNPKLRSLVVVTLGSW-NMAGSSFTRLELLRVLDLVQAKLKG 609
Query: 614 PKF---VDKLKHLRYLNLSGLNVTFLPNSITTLYNLETLILRYCLWLRK--LPKDINNLI 673
K + KL HLRYL+L VT +P S+ L L L L L R +P + +
Sbjct: 610 GKLASCIGKLIHLRYLSLEYAEVTHIPYSLGNLKLLIYLNLHISLSSRSNFVPNVLMGMQ 669
Query: 674 NLRHLDIYDCSSLTHMPK-GLGGMTSLQTMSMFVLGKNKGGDLSALNGLKSLRGLLCIKG 733
LR+L + S + K L + L+T+ F K L L G+ LR L
Sbjct: 670 ELRYLAL--PSLIERKTKLELSNLVKLETLENF---STKNSSLEDLRGMVRLRTLT---- 729
Query: 734 LQFCTTADLKNLE-----LHWDIKMDHEDALDDGDNDDEGVLEGLKPHSNIRKMIIKGYR 793
++ L+ L L + K++ +D + G++ +++++ ++ Y
Sbjct: 730 IELIEETSLETLAASIGGLKYLEKLEIDDLGSKMRTKEAGIVFDF---VHLKRLRLELYM 789
Query: 794 GMKLCDWFSSNFLGGLVSIELSHCEKLEH-LPQFDQFLYLKHLLLGYLPNIEYIDSGNSV 853
+ +F L ++ L HC E +P ++ L LK L LG+ SG +
Sbjct: 790 PRLSKE---QHFPSHLTTLYLQHCRLEEDPMPILEKLLQLKELELGHKS-----FSGKKM 849
Query: 854 SSSTTFFPSLEKLRIESMPKLKGWWKGEISFPTTILHQLSELCIFYCPLLAS-----IPQ 913
S+ FP L+KL I + + + W E S P L L IF C L +P
Sbjct: 850 VCSSCGFPQLQKLSISGLKEWEDWKVEESSMPL-----LLTLNIFDCRKLKQLPDEHLPS 909
Query: 914 HPSLESLRICGVS---VQLFQMVIRMATDLSEHSSSSSTLSKLSFLEIGTIDLEFLP--- 973
H + SL+ CG+ L ++V LSE + F ++ +DL L
Sbjct: 910 HLTAISLKKCGLEDPIPTLERLVHLKELSLSELCGRIMVCTGGGFPQLHKLDLSELDGLE 969
Query: 974 --VELFCNMTHLESLIIERCKSL--------QMSSPH--PVDEDNDVVWKKLSNLRTLRL 1033
+ +M L +L I RC L Q+ + H V+E + + K ++ L
Sbjct: 970 EWIVEDGSMPRLHTLEIRRCLKLKKLPNGFPQLQNLHLTEVEEWEEGMIVKQGSMPLLHT 1029
Query: 1034 ESILKLEYFPKSLKYITSLETLKLSNCENLVSTEGIGELISLSHLEIDRCPNLPILSEDV 1093
I P + + L T+ L V + + L L HL+ N+ +
Sbjct: 1030 LYIWHCPKLPGEQHFPSHLTTVFLLGM--YVEEDPMRILEKLLHLK-----NVSLFQSFS 1089
Query: 1094 GDLISLSHLLIWNCPKLTSLS-------EGITRLTS---LSSLCLEDCPNLVSLPQEFLH 1127
G + S P+L LS E I S L +L + CPNL LP
Sbjct: 1090 GKRMVCSG---GGFPQLQKLSIREIEWEEWIVEQGSMPLLHTLYIGVCPNLKELPD---- 1131
BLAST of Cucsa.128100 vs. TAIR10
Match:
AT3G50950.2 (AT3G50950.2 HOPZ-ACTIVATED RESISTANCE 1)
HSP 1 Score: 263.1 bits (671), Expect = 8.0e-70
Identity = 211/766 (27.55%), Postives = 370/766 (48.30%), Query Frame = 1
Query: 34 ELDKLQNTLSAIKAVLLDAEEQQSKSHTVKDWIAKIKDVFYDIDDLIDEFSYETLRRQVL 93
+L+ LQ+ L +++ L DAE Q+ + T++ +A ++++ Y+ +D++ +
Sbjct: 30 QLEDLQSELKYMQSFLKDAERQKRTNETLRTLVADLRELVYEAEDILVDCQLAD------ 89
Query: 94 TKDRTITKQVRIFFSKSN--QIAFGFKMGQTIKKVREKLDAIAA-IKAQLHLSVCAREVR 153
D + + S+ + ++ +K + ++++ E++ I + ++ + R
Sbjct: 90 GDDGNEQRSSNAWLSRLHPARVPLQYKKSKRLQEINERITKIKSQVEPYFEFITPSNVGR 149
Query: 154 DNEPRKVRETSSFIPEGEIIGRDEDRKSVMDFLLNTSNITKDNVEVVSIVGMGGLGKTAL 213
DN R +S +++G + D++ + ++L +++ + +++ VGMGGLGKT +
Sbjct: 150 DNGTD--RWSSPVYDHTQVVGLEGDKRKIKEWLFRSND---SQLLIMAFVGMGGLGKTTI 209
Query: 214 AQTVYNDEKINNRFKWKIWVCISQEFDIKVIVEKILESITKTKQESLQLDILQSMLQEKI 273
AQ V+ND++I +RF+ +IWV +SQ F + I+ IL ++ + L +Q+ +
Sbjct: 210 AQEVFNDKEIEHRFERRIWVSVSQTFTEEQIMRSILRNLGDASVGD-DIGTLLRKIQQYL 269
Query: 274 YGKKYLLVMDDVWNVDHEKWIGLKRFLMGGASGSKILVTTRNLQTA---QASDTVWFHHL 333
GK+YL+VMDDVW+ + W + + L G GS ++VTTR+ A QA D H
Sbjct: 270 LGKRYLIVMDDVWDKNLSWWDKIYQGLPRGQGGS-VIVTTRSESVAKRVQARDDKT-HRP 329
Query: 334 KELDKDNSWALFRKMAFLNKEEELENSNLVRIGKEIVAKLKGYPLSIRVVGRLLYFKN-T 393
+ L DNSW LF +AF + E L +GKEIV K KG PL+I+ VG LL K+
Sbjct: 330 ELLSPDNSWLLFCNVAFAANDGTCERPELEDVGKEIVTKCKGLPLTIKAVGGLLLCKDHV 389
Query: 394 EMDWSSFKDN---ELDSILQEDDQIQPILKISFNHLPPKLKQCFTYCALFPKDYEFKKNG 453
+W ++ EL E D + L++S++ LP LK C +L+P+D K
Sbjct: 390 YHEWRRIAEHFQDELRGNTSETDNVMSSLQLSYDELPSHLKSCILTLSLYPEDCVIPKQQ 449
Query: 454 LVKQWMAQGFIQAHNKKAIEDVGDDYFQELVGRSFFQDIRKNKWGDLKYCKMHDLLHDLA 513
LV W+ +GF+ N ++ + G+D F L R + + K G + CK+HD++ DL
Sbjct: 450 LVHGWIGEGFVMWRNGRSATESGEDCFSGLTNRCLIEVVDKTYSGTIITCKIHDMVRDLV 509
Query: 514 CSIGENECVVVSDDV--------GSIDKRTRHASFLLSKRLTREVVSKSSIEVTSLRTLD 573
I + + + + G+ D++ + L R VV S+ + + L+
Sbjct: 510 IDIAKKDSFSNPEGLNCRHLGISGNFDEKQIKVNHKL-----RGVV--STTKTGEVNKLN 569
Query: 574 IDSRASFRSFKKTCHMNLFQLRTLNLDRCCCHPP--KFVDK---LKHLRYLNLSGLN-VT 633
D F K LR L++ + P + +D+ L+HL L+LS + +
Sbjct: 570 SDLAKKFTDCK--------YLRVLDISKSIFDAPLSEILDEIASLQHLACLSLSNTHPLI 629
Query: 634 FLPNSITTLYNLETLILRYCLWLRKLPKDINNLINLRHLDIYDCSSLTHMPKGLGGMTSL 693
P S+ L+NL+ L YC L++L I L LD+ +C SL PKG+G + L
Sbjct: 630 QFPRSMEDLHNLQILDASYCQNLKQLQPCIVLFKKLLVLDMTNCGSLECFPKGIGSLVKL 689
Query: 694 QTMSMFVLGK-NKGGDLSALNGLKSLR--GLLCIKGLQFCTTA--DLKNLELHWDIKMDH 753
+ + F + N G LS + L +LR GL +G Q L NL I ++
Sbjct: 690 EVLLGFKPARSNNGCKLSEVKNLTNLRKLGLSLTRGDQIEEEELDSLINLSKLMSISINC 749
Query: 754 EDALDDGDNDDEGVLEGLKPHSNIRKMIIKGYRGMKLCDWFSSNFL 771
D+ D D ++ L P + ++ ++ Y G W S + L
Sbjct: 750 YDSYGD---DLITKIDALTPPHQLHELSLQFYPGKSSPSWLSPHKL 763
BLAST of Cucsa.128100 vs. TAIR10
Match:
AT3G46730.1 (AT3G46730.1 NB-ARC domain-containing disease resistance protein)
HSP 1 Score: 256.5 bits (654), Expect = 7.5e-68
Identity = 188/647 (29.06%), Postives = 317/647 (49.00%), Query Frame = 1
Query: 9 VAASVITKLGSSALRELGSLWGVNDELDKLQNTLSAIKAVLLDAEEQQSKSHTVKDWIAK 68
V V+ K+G + E+ +L GV D+L++L+ L+ I L D E ++ + K+W
Sbjct: 5 VTGFVLNKIGGYLINEVLALMGVKDDLEELKTELTCIHGYLKDVEAREREDEVSKEWTKL 64
Query: 69 IKDVFYDIDDLIDEFSYETLRRQVLTKDRTITKQVRIFFSKSNQIAFGFKMGQTIKKVRE 128
+ D+ YDI+D++D + + ++R++ + + +K + + + + I+ ++
Sbjct: 65 VLDIAYDIEDVLDTYFLKL-------EERSLRRGLLRLTNKIGKKRDAYNIVEDIRTLKR 124
Query: 129 KLDAIAAIKAQLHLSVCAREVRDN----EPRKVRETSSFIPEGEIIGRDEDRKSVMDFLL 188
++ I + + +N R++R E ++G ++D K ++ LL
Sbjct: 125 RILDITRKRETFGIGSFNEPRGENITNVRVRQLRRAPPVDQEELVVGLEDDVKILLVKLL 184
Query: 189 NTSNITKDNVEVVSIVGMGGLGKTALAQTVYNDEKINNRFKWKIWVCISQEFDIKVIVEK 248
+ + KD ++SI GMGGLGKTALA+ +YN + RF + W +SQE+ + I+ +
Sbjct: 185 SDNE--KDKSYIISIFGMGGLGKTALARKLYNSGDVKRRFDCRAWTYVSQEYKTRDILIR 244
Query: 249 ILESITKTKQESLQL-------DILQSMLQEKIYGKKYLLVMDDVWNVDHEKWIGLKRFL 308
I+ S+ E ++ + L+ L + GK Y++V+DDVW D + W LKR L
Sbjct: 245 IIRSLGIVSAEEMEKIKMFEEDEELEVYLYGLLEGKNYMVVVDDVW--DPDAWESLKRAL 304
Query: 309 MGGASGSKILVTTRNLQTAQASD-TVWFHHLKELDKDNSWALFRKMAFLNKEEELENSNL 368
GSK+++TTR A+ + TV+ H L+ L + SW LF + AF N E+ + +L
Sbjct: 305 PCDHRGSKVIITTRIRAIAEGVEGTVYAHKLRFLTFEESWTLFERKAFSNIEK--VDEDL 364
Query: 369 VRIGKEIVAKLKGYPLSIRVVGRLLYFKNTE-------MDWSSFKDNELDSILQEDDQIQ 428
R GKE+V K G PL+I V+ LL K T W KDN + I
Sbjct: 365 QRTGKEMVKKCGGLPLAIVVLSGLLSRKRTNEWHEVCASLWRRLKDNSI--------HIS 424
Query: 429 PILKISFNHLPPKLKQCFTYCALFPKDYEFKKNGLVKQWMAQGFIQAHNKKAIEDVGDDY 488
+ +SF + +LK CF Y ++FP+DYE K L+ +A+GFIQ + +EDV Y
Sbjct: 425 TVFDLSFKEMRHELKLCFLYFSVFPEDYEIKVEKLIHLLVAEGFIQEDEEMMMEDVARCY 484
Query: 489 FQELVGRSFFQDIRKNKWGDLKYCKMHDLLHDLACSIGENECVVVSDDVGSIDKRTRHAS 548
ELV RS + R + G + C++HDLL DLA + V ++ +H+S
Sbjct: 485 IDELVDRSLVKAERIER-GKVMSCRIHDLLRDLAIKKAKELNFV------NVYNEKQHSS 544
Query: 549 FLLSKRLTREVVS-----KSSIEVTSLRTLDIDSRASFRSFKKTCHMNLFQLRTLNLD-- 608
+ + + +++ + L I R F + T ++ L LR LN++
Sbjct: 545 DICRREVVHHLMNDYYLCDRRVNKRMRSFLFIGERRGF-GYVNTTNLKLKLLRVLNMEGL 604
Query: 609 -----RCCCHPPKFVDKLKHLRYLNLSGLNVTFLPNSITTLYNLETL 625
P + +L HLRYL ++ V+ LP SI+ L L+TL
Sbjct: 605 LFVSKNISNTLPDVIGELIHLRYLGIADTYVSILPASISNLRFLQTL 622
BLAST of Cucsa.128100 vs. NCBI nr
Match:
gi|778728529|ref|XP_011659434.1| (PREDICTED: putative disease resistance protein RGA1 isoform X1 [Cucumis sativus])
HSP 1 Score: 2260.3 bits (5856), Expect = 0.0e+00
Identity = 1130/1142 (98.95%), Postives = 1130/1142 (98.95%), Query Frame = 1
Query: 1 MADSVLFNVAASVITKLGSSALRELGSLWGVNDELDKLQNTLSAIKAVLLDAEEQQSKSH 60
MADSVLFNVAASVITKLGSSALRELGSLWGVNDELDKLQNTLSAIKAVLLDAEEQQSKSH
Sbjct: 1 MADSVLFNVAASVITKLGSSALRELGSLWGVNDELDKLQNTLSAIKAVLLDAEEQQSKSH 60
Query: 61 TVKDWIAKIKDVFYDIDDLIDEFSYETLRRQVLTKDRTITKQVRIFFSKSNQIAFGFKMG 120
TVKDWIAKIKDVFYDIDDLIDEFSYETLRRQVLTKDRTITKQVRIFFSKSNQIAFGFKMG
Sbjct: 61 TVKDWIAKIKDVFYDIDDLIDEFSYETLRRQVLTKDRTITKQVRIFFSKSNQIAFGFKMG 120
Query: 121 QTIKKVREKLDAIAAIKAQLHLSVCAREVRDNEPRKVRETSSFIPEGEIIGRDEDRKSVM 180
QTIKKVREKLDAIAAIKAQLHLSVCAREVRDNEPRKVRETSSFIPEGEIIGRDEDRKSVM
Sbjct: 121 QTIKKVREKLDAIAAIKAQLHLSVCAREVRDNEPRKVRETSSFIPEGEIIGRDEDRKSVM 180
Query: 181 DFLLNTSNITKDNVEVVSIVGMGGLGKTALAQTVYNDEKINNRFKWKIWVCISQEFDIKV 240
DFLLNTSNITKDNVEVVSIVGMGGLGKTALAQTVYNDEKINNRFKWKIWVCISQEFDIKV
Sbjct: 181 DFLLNTSNITKDNVEVVSIVGMGGLGKTALAQTVYNDEKINNRFKWKIWVCISQEFDIKV 240
Query: 241 IVEKILESITKTKQESLQLDILQSMLQEKIYGKKYLLVMDDVWNVDHEKWIGLKRFLMGG 300
IVEKILESITKTKQESLQLDILQSMLQEKIYGKKYLLVMDDVWNVDHEKWIGLKRFLMGG
Sbjct: 241 IVEKILESITKTKQESLQLDILQSMLQEKIYGKKYLLVMDDVWNVDHEKWIGLKRFLMGG 300
Query: 301 ASGSKILVTTRNLQTAQASDTVWFHHLKELDKDNSWALFRKMAFLNKEEELENSNLVRIG 360
ASGSKILVTTRNLQTAQASDTVWFHHLKELDKDNSWALFRKMAFLNKEEELENSNLVRIG
Sbjct: 301 ASGSKILVTTRNLQTAQASDTVWFHHLKELDKDNSWALFRKMAFLNKEEELENSNLVRIG 360
Query: 361 KEIVAKLKGYPLSIRVVGRLLYFKNTEMDWSSFKDNELDSILQEDDQIQPILKISFNHLP 420
KEIVAKLKGYPLSIRVVGRLLYFKNTEMDWSSFKDNELDSILQEDDQIQPILKISFNHLP
Sbjct: 361 KEIVAKLKGYPLSIRVVGRLLYFKNTEMDWSSFKDNELDSILQEDDQIQPILKISFNHLP 420
Query: 421 PKLKQCFTYCALFPKDYEFKKNGLVKQWMAQGFIQAHNKKAIEDVGDDYFQELVGRSFFQ 480
PKLKQCFTYCALFPKDYEFKKNGLVKQWMAQGFIQAHNKKAIEDVGDDYFQELVGRSFFQ
Sbjct: 421 PKLKQCFTYCALFPKDYEFKKNGLVKQWMAQGFIQAHNKKAIEDVGDDYFQELVGRSFFQ 480
Query: 481 DIRKNKWGDLKYCKMHDLLHDLACSIGENECVVVSDDVGSIDKRTRHASFLLSKRLTREV 540
DIRKNKWGDLKYCKMHDLLHDLACSIGENECVVVSDDVGSIDKRTRHASFLLSKRLTREV
Sbjct: 481 DIRKNKWGDLKYCKMHDLLHDLACSIGENECVVVSDDVGSIDKRTRHASFLLSKRLTREV 540
Query: 541 VSKSSIEVTSLRTLDIDSRASFRSFKKTCHMNLFQLRTLNLDRCCCHPPKFVDKLKHLRY 600
VSKSSIEVTSLRTLDIDSRASFRSFKKTCHMNLFQLRTLNLDRCCCHPPKFVDKLKHLRY
Sbjct: 541 VSKSSIEVTSLRTLDIDSRASFRSFKKTCHMNLFQLRTLNLDRCCCHPPKFVDKLKHLRY 600
Query: 601 LNLSGLNVTFLPNSITTLYNLETLILRYCLWLRKLPKDINNLINLRHLDIYDCSSLTHMP 660
LNLSGLNVTFLPNSITTLYNLETLILRYCLWLRKLPKDINNLINLRHLDIYDCSSLTHMP
Sbjct: 601 LNLSGLNVTFLPNSITTLYNLETLILRYCLWLRKLPKDINNLINLRHLDIYDCSSLTHMP 660
Query: 661 KGLGGMTSLQTMSMFVLGKNKGGDLSALNGLKSLRGLLCIKGLQFCTTADLKN------- 720
KGLGGMTSLQTMSMFVLGKNKGGDLSALNGLKSLRGLLCIKGLQFCTTADLKN
Sbjct: 661 KGLGGMTSLQTMSMFVLGKNKGGDLSALNGLKSLRGLLCIKGLQFCTTADLKNVSYLKEM 720
Query: 721 -----LELHWDIKMDHEDALDDGDNDDEGVLEGLKPHSNIRKMIIKGYRGMKLCDWFSSN 780
LELHWDIKMDHEDALDDGDNDDEGVLEGLKPHSNIRKMIIKGYRGMKLCDWFSSN
Sbjct: 721 YGIQKLELHWDIKMDHEDALDDGDNDDEGVLEGLKPHSNIRKMIIKGYRGMKLCDWFSSN 780
Query: 781 FLGGLVSIELSHCEKLEHLPQFDQFLYLKHLLLGYLPNIEYIDSGNSVSSSTTFFPSLEK 840
FLGGLVSIELSHCEKLEHLPQFDQFLYLKHLLLGYLPNIEYIDSGNSVSSSTTFFPSLEK
Sbjct: 781 FLGGLVSIELSHCEKLEHLPQFDQFLYLKHLLLGYLPNIEYIDSGNSVSSSTTFFPSLEK 840
Query: 841 LRIESMPKLKGWWKGEISFPTTILHQLSELCIFYCPLLASIPQHPSLESLRICGVSVQLF 900
LRIESMPKLKGWWKGEISFPTTILHQLSELCIFYCPLLASIPQHPSLESLRICGVSVQLF
Sbjct: 841 LRIESMPKLKGWWKGEISFPTTILHQLSELCIFYCPLLASIPQHPSLESLRICGVSVQLF 900
Query: 901 QMVIRMATDLSEHSSSSSTLSKLSFLEIGTIDLEFLPVELFCNMTHLESLIIERCKSLQM 960
QMVIRMATDLSEHSSSSSTLSKLSFLEIGTIDLEFLPVELFCNMTHLESLIIERCKSLQM
Sbjct: 901 QMVIRMATDLSEHSSSSSTLSKLSFLEIGTIDLEFLPVELFCNMTHLESLIIERCKSLQM 960
Query: 961 SSPHPVDEDNDVVWKKLSNLRTLRLESILKLEYFPKSLKYITSLETLKLSNCENLVSTEG 1020
SSPHPVDEDNDVVWKKLSNLRTLRLESILKLEYFPKSLKYITSLETLKLSNCENLVSTEG
Sbjct: 961 SSPHPVDEDNDVVWKKLSNLRTLRLESILKLEYFPKSLKYITSLETLKLSNCENLVSTEG 1020
Query: 1021 IGELISLSHLEIDRCPNLPILSEDVGDLISLSHLLIWNCPKLTSLSEGITRLTSLSSLCL 1080
IGELISLSHLEIDRCPNLPILSEDVGDLISLSHLLIWNCPKLTSLSEGITRLTSLSSLCL
Sbjct: 1021 IGELISLSHLEIDRCPNLPILSEDVGDLISLSHLLIWNCPKLTSLSEGITRLTSLSSLCL 1080
Query: 1081 EDCPNLVSLPQEFLHHHSSLPGGRFLRILNCPKLQIQDKKQKEEEEEDQEDWNELIHVLT 1131
EDCPNLVSLPQEFLHHHSSLPGGRFLRILNCPKLQIQDKKQKEEEEEDQEDWNELIHVLT
Sbjct: 1081 EDCPNLVSLPQEFLHHHSSLPGGRFLRILNCPKLQIQDKKQKEEEEEDQEDWNELIHVLT 1140
BLAST of Cucsa.128100 vs. NCBI nr
Match:
gi|778728533|ref|XP_011659435.1| (PREDICTED: putative disease resistance protein RGA1 isoform X2 [Cucumis sativus])
HSP 1 Score: 2260.3 bits (5856), Expect = 0.0e+00
Identity = 1130/1142 (98.95%), Postives = 1130/1142 (98.95%), Query Frame = 1
Query: 1 MADSVLFNVAASVITKLGSSALRELGSLWGVNDELDKLQNTLSAIKAVLLDAEEQQSKSH 60
MADSVLFNVAASVITKLGSSALRELGSLWGVNDELDKLQNTLSAIKAVLLDAEEQQSKSH
Sbjct: 1 MADSVLFNVAASVITKLGSSALRELGSLWGVNDELDKLQNTLSAIKAVLLDAEEQQSKSH 60
Query: 61 TVKDWIAKIKDVFYDIDDLIDEFSYETLRRQVLTKDRTITKQVRIFFSKSNQIAFGFKMG 120
TVKDWIAKIKDVFYDIDDLIDEFSYETLRRQVLTKDRTITKQVRIFFSKSNQIAFGFKMG
Sbjct: 61 TVKDWIAKIKDVFYDIDDLIDEFSYETLRRQVLTKDRTITKQVRIFFSKSNQIAFGFKMG 120
Query: 121 QTIKKVREKLDAIAAIKAQLHLSVCAREVRDNEPRKVRETSSFIPEGEIIGRDEDRKSVM 180
QTIKKVREKLDAIAAIKAQLHLSVCAREVRDNEPRKVRETSSFIPEGEIIGRDEDRKSVM
Sbjct: 121 QTIKKVREKLDAIAAIKAQLHLSVCAREVRDNEPRKVRETSSFIPEGEIIGRDEDRKSVM 180
Query: 181 DFLLNTSNITKDNVEVVSIVGMGGLGKTALAQTVYNDEKINNRFKWKIWVCISQEFDIKV 240
DFLLNTSNITKDNVEVVSIVGMGGLGKTALAQTVYNDEKINNRFKWKIWVCISQEFDIKV
Sbjct: 181 DFLLNTSNITKDNVEVVSIVGMGGLGKTALAQTVYNDEKINNRFKWKIWVCISQEFDIKV 240
Query: 241 IVEKILESITKTKQESLQLDILQSMLQEKIYGKKYLLVMDDVWNVDHEKWIGLKRFLMGG 300
IVEKILESITKTKQESLQLDILQSMLQEKIYGKKYLLVMDDVWNVDHEKWIGLKRFLMGG
Sbjct: 241 IVEKILESITKTKQESLQLDILQSMLQEKIYGKKYLLVMDDVWNVDHEKWIGLKRFLMGG 300
Query: 301 ASGSKILVTTRNLQTAQASDTVWFHHLKELDKDNSWALFRKMAFLNKEEELENSNLVRIG 360
ASGSKILVTTRNLQTAQASDTVWFHHLKELDKDNSWALFRKMAFLNKEEELENSNLVRIG
Sbjct: 301 ASGSKILVTTRNLQTAQASDTVWFHHLKELDKDNSWALFRKMAFLNKEEELENSNLVRIG 360
Query: 361 KEIVAKLKGYPLSIRVVGRLLYFKNTEMDWSSFKDNELDSILQEDDQIQPILKISFNHLP 420
KEIVAKLKGYPLSIRVVGRLLYFKNTEMDWSSFKDNELDSILQEDDQIQPILKISFNHLP
Sbjct: 361 KEIVAKLKGYPLSIRVVGRLLYFKNTEMDWSSFKDNELDSILQEDDQIQPILKISFNHLP 420
Query: 421 PKLKQCFTYCALFPKDYEFKKNGLVKQWMAQGFIQAHNKKAIEDVGDDYFQELVGRSFFQ 480
PKLKQCFTYCALFPKDYEFKKNGLVKQWMAQGFIQAHNKKAIEDVGDDYFQELVGRSFFQ
Sbjct: 421 PKLKQCFTYCALFPKDYEFKKNGLVKQWMAQGFIQAHNKKAIEDVGDDYFQELVGRSFFQ 480
Query: 481 DIRKNKWGDLKYCKMHDLLHDLACSIGENECVVVSDDVGSIDKRTRHASFLLSKRLTREV 540
DIRKNKWGDLKYCKMHDLLHDLACSIGENECVVVSDDVGSIDKRTRHASFLLSKRLTREV
Sbjct: 481 DIRKNKWGDLKYCKMHDLLHDLACSIGENECVVVSDDVGSIDKRTRHASFLLSKRLTREV 540
Query: 541 VSKSSIEVTSLRTLDIDSRASFRSFKKTCHMNLFQLRTLNLDRCCCHPPKFVDKLKHLRY 600
VSKSSIEVTSLRTLDIDSRASFRSFKKTCHMNLFQLRTLNLDRCCCHPPKFVDKLKHLRY
Sbjct: 541 VSKSSIEVTSLRTLDIDSRASFRSFKKTCHMNLFQLRTLNLDRCCCHPPKFVDKLKHLRY 600
Query: 601 LNLSGLNVTFLPNSITTLYNLETLILRYCLWLRKLPKDINNLINLRHLDIYDCSSLTHMP 660
LNLSGLNVTFLPNSITTLYNLETLILRYCLWLRKLPKDINNLINLRHLDIYDCSSLTHMP
Sbjct: 601 LNLSGLNVTFLPNSITTLYNLETLILRYCLWLRKLPKDINNLINLRHLDIYDCSSLTHMP 660
Query: 661 KGLGGMTSLQTMSMFVLGKNKGGDLSALNGLKSLRGLLCIKGLQFCTTADLKN------- 720
KGLGGMTSLQTMSMFVLGKNKGGDLSALNGLKSLRGLLCIKGLQFCTTADLKN
Sbjct: 661 KGLGGMTSLQTMSMFVLGKNKGGDLSALNGLKSLRGLLCIKGLQFCTTADLKNVSYLKEM 720
Query: 721 -----LELHWDIKMDHEDALDDGDNDDEGVLEGLKPHSNIRKMIIKGYRGMKLCDWFSSN 780
LELHWDIKMDHEDALDDGDNDDEGVLEGLKPHSNIRKMIIKGYRGMKLCDWFSSN
Sbjct: 721 YGIQKLELHWDIKMDHEDALDDGDNDDEGVLEGLKPHSNIRKMIIKGYRGMKLCDWFSSN 780
Query: 781 FLGGLVSIELSHCEKLEHLPQFDQFLYLKHLLLGYLPNIEYIDSGNSVSSSTTFFPSLEK 840
FLGGLVSIELSHCEKLEHLPQFDQFLYLKHLLLGYLPNIEYIDSGNSVSSSTTFFPSLEK
Sbjct: 781 FLGGLVSIELSHCEKLEHLPQFDQFLYLKHLLLGYLPNIEYIDSGNSVSSSTTFFPSLEK 840
Query: 841 LRIESMPKLKGWWKGEISFPTTILHQLSELCIFYCPLLASIPQHPSLESLRICGVSVQLF 900
LRIESMPKLKGWWKGEISFPTTILHQLSELCIFYCPLLASIPQHPSLESLRICGVSVQLF
Sbjct: 841 LRIESMPKLKGWWKGEISFPTTILHQLSELCIFYCPLLASIPQHPSLESLRICGVSVQLF 900
Query: 901 QMVIRMATDLSEHSSSSSTLSKLSFLEIGTIDLEFLPVELFCNMTHLESLIIERCKSLQM 960
QMVIRMATDLSEHSSSSSTLSKLSFLEIGTIDLEFLPVELFCNMTHLESLIIERCKSLQM
Sbjct: 901 QMVIRMATDLSEHSSSSSTLSKLSFLEIGTIDLEFLPVELFCNMTHLESLIIERCKSLQM 960
Query: 961 SSPHPVDEDNDVVWKKLSNLRTLRLESILKLEYFPKSLKYITSLETLKLSNCENLVSTEG 1020
SSPHPVDEDNDVVWKKLSNLRTLRLESILKLEYFPKSLKYITSLETLKLSNCENLVSTEG
Sbjct: 961 SSPHPVDEDNDVVWKKLSNLRTLRLESILKLEYFPKSLKYITSLETLKLSNCENLVSTEG 1020
Query: 1021 IGELISLSHLEIDRCPNLPILSEDVGDLISLSHLLIWNCPKLTSLSEGITRLTSLSSLCL 1080
IGELISLSHLEIDRCPNLPILSEDVGDLISLSHLLIWNCPKLTSLSEGITRLTSLSSLCL
Sbjct: 1021 IGELISLSHLEIDRCPNLPILSEDVGDLISLSHLLIWNCPKLTSLSEGITRLTSLSSLCL 1080
Query: 1081 EDCPNLVSLPQEFLHHHSSLPGGRFLRILNCPKLQIQDKKQKEEEEEDQEDWNELIHVLT 1131
EDCPNLVSLPQEFLHHHSSLPGGRFLRILNCPKLQIQDKKQKEEEEEDQEDWNELIHVLT
Sbjct: 1081 EDCPNLVSLPQEFLHHHSSLPGGRFLRILNCPKLQIQDKKQKEEEEEDQEDWNELIHVLT 1140
BLAST of Cucsa.128100 vs. NCBI nr
Match:
gi|700189869|gb|KGN45102.1| (hypothetical protein Csa_7G425940 [Cucumis sativus])
HSP 1 Score: 1580.1 bits (4090), Expect = 0.0e+00
Identity = 781/793 (98.49%), Postives = 781/793 (98.49%), Query Frame = 1
Query: 350 ELENSNLVRIGKEIVAKLKGYPLSIRVVGRLLYFKNTEMDWSSFKDNELDSILQEDDQIQ 409
ELENSNLVRIGKEIVAKLKGYPLSIRVVGRLLYFKNTEMDWSSFKDNELDSILQEDDQIQ
Sbjct: 171 ELENSNLVRIGKEIVAKLKGYPLSIRVVGRLLYFKNTEMDWSSFKDNELDSILQEDDQIQ 230
Query: 410 PILKISFNHLPPKLKQCFTYCALFPKDYEFKKNGLVKQWMAQGFIQAHNKKAIEDVGDDY 469
PILKISFNHLPPKLKQCFTYCALFPKDYEFKKNGLVKQWMAQGFIQAHNKKAIEDVGDDY
Sbjct: 231 PILKISFNHLPPKLKQCFTYCALFPKDYEFKKNGLVKQWMAQGFIQAHNKKAIEDVGDDY 290
Query: 470 FQELVGRSFFQDIRKNKWGDLKYCKMHDLLHDLACSIGENECVVVSDDVGSIDKRTRHAS 529
FQELVGRSFFQDIRKNKWGDLKYCKMHDLLHDLACSIGENECVVVSDDVGSIDKRTRHAS
Sbjct: 291 FQELVGRSFFQDIRKNKWGDLKYCKMHDLLHDLACSIGENECVVVSDDVGSIDKRTRHAS 350
Query: 530 FLLSKRLTREVVSKSSIEVTSLRTLDIDSRASFRSFKKTCHMNLFQLRTLNLDRCCCHPP 589
FLLSKRLTREVVSKSSIEVTSLRTLDIDSRASFRSFKKTCHMNLFQLRTLNLDRCCCHPP
Sbjct: 351 FLLSKRLTREVVSKSSIEVTSLRTLDIDSRASFRSFKKTCHMNLFQLRTLNLDRCCCHPP 410
Query: 590 KFVDKLKHLRYLNLSGLNVTFLPNSITTLYNLETLILRYCLWLRKLPKDINNLINLRHLD 649
KFVDKLKHLRYLNLSGLNVTFLPNSITTLYNLETLILRYCLWLRKLPKDINNLINLRHLD
Sbjct: 411 KFVDKLKHLRYLNLSGLNVTFLPNSITTLYNLETLILRYCLWLRKLPKDINNLINLRHLD 470
Query: 650 IYDCSSLTHMPKGLGGMTSLQTMSMFVLGKNKGGDLSALNGLKSLRGLLCIKGLQFCTTA 709
IYDCSSLTHMPKGLGGMTSLQTMSMFVLGKNKGGDLSALNGLKSLRGLLCIKGLQFCTTA
Sbjct: 471 IYDCSSLTHMPKGLGGMTSLQTMSMFVLGKNKGGDLSALNGLKSLRGLLCIKGLQFCTTA 530
Query: 710 DLKN------------LELHWDIKMDHEDALDDGDNDDEGVLEGLKPHSNIRKMIIKGYR 769
DLKN LELHWDIKMDHEDALDDGDNDDEGVLEGLKPHSNIRKMIIKGYR
Sbjct: 531 DLKNVSYLKEMYGIQKLELHWDIKMDHEDALDDGDNDDEGVLEGLKPHSNIRKMIIKGYR 590
Query: 770 GMKLCDWFSSNFLGGLVSIELSHCEKLEHLPQFDQFLYLKHLLLGYLPNIEYIDSGNSVS 829
GMKLCDWFSSNFLGGLVSIELSHCEKLEHLPQFDQFLYLKHLLLGYLPNIEYIDSGNSVS
Sbjct: 591 GMKLCDWFSSNFLGGLVSIELSHCEKLEHLPQFDQFLYLKHLLLGYLPNIEYIDSGNSVS 650
Query: 830 SSTTFFPSLEKLRIESMPKLKGWWKGEISFPTTILHQLSELCIFYCPLLASIPQHPSLES 889
SSTTFFPSLEKLRIESMPKLKGWWKGEISFPTTILHQLSELCIFYCPLLASIPQHPSLES
Sbjct: 651 SSTTFFPSLEKLRIESMPKLKGWWKGEISFPTTILHQLSELCIFYCPLLASIPQHPSLES 710
Query: 890 LRICGVSVQLFQMVIRMATDLSEHSSSSSTLSKLSFLEIGTIDLEFLPVELFCNMTHLES 949
LRICGVSVQLFQMVIRMATDLSEHSSSSSTLSKLSFLEIGTIDLEFLPVELFCNMTHLES
Sbjct: 711 LRICGVSVQLFQMVIRMATDLSEHSSSSSTLSKLSFLEIGTIDLEFLPVELFCNMTHLES 770
Query: 950 LIIERCKSLQMSSPHPVDEDNDVVWKKLSNLRTLRLESILKLEYFPKSLKYITSLETLKL 1009
LIIERCKSLQMSSPHPVDEDNDVVWKKLSNLRTLRLESILKLEYFPKSLKYITSLETLKL
Sbjct: 771 LIIERCKSLQMSSPHPVDEDNDVVWKKLSNLRTLRLESILKLEYFPKSLKYITSLETLKL 830
Query: 1010 SNCENLVSTEGIGELISLSHLEIDRCPNLPILSEDVGDLISLSHLLIWNCPKLTSLSEGI 1069
SNCENLVSTEGIGELISLSHLEIDRCPNLPILSEDVGDLISLSHLLIWNCPKLTSLSEGI
Sbjct: 831 SNCENLVSTEGIGELISLSHLEIDRCPNLPILSEDVGDLISLSHLLIWNCPKLTSLSEGI 890
Query: 1070 TRLTSLSSLCLEDCPNLVSLPQEFLHHHSSLPGGRFLRILNCPKLQIQDKKQKEEEEEDQ 1129
TRLTSLSSLCLEDCPNLVSLPQEFLHHHSSLPGGRFLRILNCPKLQIQDKKQKEEEEEDQ
Sbjct: 891 TRLTSLSSLCLEDCPNLVSLPQEFLHHHSSLPGGRFLRILNCPKLQIQDKKQKEEEEEDQ 950
Query: 1130 EDWNELIHVLTGC 1131
EDWNELIHVLTGC
Sbjct: 951 EDWNELIHVLTGC 963
BLAST of Cucsa.128100 vs. NCBI nr
Match:
gi|700189869|gb|KGN45102.1| (hypothetical protein Csa_7G425940 [Cucumis sativus])
HSP 1 Score: 328.6 bits (841), Expect = 4.4e-86
Identity = 175/198 (88.38%), Postives = 179/198 (90.40%), Query Frame = 1
Query: 1 MADSVLFNVAASVITKLGSSALRELGSLWGVNDELDKLQNTLSAIKAVLLDAEEQQSKSH 60
MADSVLFNVAASVITKLGSSALRELGSLWGVNDELDKLQNTLSAIKAVLLDAEEQQSKSH
Sbjct: 1 MADSVLFNVAASVITKLGSSALRELGSLWGVNDELDKLQNTLSAIKAVLLDAEEQQSKSH 60
Query: 61 TVKDWIAKIKDVFYDIDDLIDEFSYETLRRQVLTKDRTITKQVRIFFSKSNQIAFGFKMG 120
TVKDWIAKIKDVFYDIDDLIDEFSYETLRRQVLTKDRTITKQVRIFFSKSNQIAFGFKMG
Sbjct: 61 TVKDWIAKIKDVFYDIDDLIDEFSYETLRRQVLTKDRTITKQVRIFFSKSNQIAFGFKMG 120
Query: 121 QTIKKVREKLDAIAAIKAQLHLSVCAREVRDNEPRKVRETSSFIPEGEIIGRDEDRKSVM 180
QTIKKVREKLDAIAAIKAQLHLSVCAREVRDNEPRKVRETSSFIPEGEII
Sbjct: 121 QTIKKVREKLDAIAAIKAQLHLSVCAREVRDNEPRKVRETSSFIPEGEIIE--------- 180
Query: 181 DFLLNTSNITKDNVEVVS 199
L SN+ + E+V+
Sbjct: 181 ---LENSNLVRIGKEIVA 186
HSP 2 Score: 1408.3 bits (3644), Expect = 0.0e+00
Identity = 755/1126 (67.05%), Postives = 871/1126 (77.35%), Query Frame = 1
Query: 1 MADSVLFNVAASVITKLGSSALRELGSLWGVNDELDKLQNTLSAIKAVLLDAEEQQSKSH 60
MADS+LFNVAA+VITKLGSSALRELGSLWGVNDEL KLQN LSAIKAVLLDAEEQQS SH
Sbjct: 27 MADSILFNVAANVITKLGSSALRELGSLWGVNDELGKLQNILSAIKAVLLDAEEQQSVSH 86
Query: 61 TVKDWIAKIKDVFYDIDDLIDEFSYETLRRQVLTKDRTITKQVRIFFSKSNQIAFGFKMG 120
VKDWI+K++DVFYD+DDLIDEFSYETLRRQVLTKDRTITKQV IFFSKSNQ++FG KM
Sbjct: 87 AVKDWISKLRDVFYDVDDLIDEFSYETLRRQVLTKDRTITKQVCIFFSKSNQVSFGHKMS 146
Query: 121 QTIKKVREKLDAIAAIKAQLHLSVCAREVRDNEPRKVRETSSFIPEGEIIGRDEDRKSVM 180
Q IK+VREKLDAIA K QLHLSV RE RD+E RK+RET SFIP+GE+IGRD+D+K+++
Sbjct: 147 QKIKQVREKLDAIANDKTQLHLSVRMRETRDDELRKMRETCSFIPKGEVIGRDDDKKAII 206
Query: 181 DFLLNTSNITKDNVEVVSIVGMGGLGKTALAQTVYNDEKINNRFKWKIWVCISQEFDIKV 240
DFLL+T N +DNVEVVSIVGMGGLGKTA+AQ+VYNDEKIN FK K+WVCISQEFDIKV
Sbjct: 207 DFLLDT-NTMEDNVEVVSIVGMGGLGKTAVAQSVYNDEKINEHFKLKLWVCISQEFDIKV 266
Query: 241 IVEKILESITKTKQESLQLDILQSMLQEKIYGKKYLLVMDDVWNVDHEKWIGLKRFLMGG 300
IVEKI+E I K K +SLQLDILQSMLQEKI GKKYLLVMDDVWN HE W+ LKRFLMGG
Sbjct: 267 IVEKIIEFIAKKKPDSLQLDILQSMLQEKIDGKKYLLVMDDVWNESHETWVSLKRFLMGG 326
Query: 301 ASGSKILVTTRNLQTAQASDTVWFHHLKELDKDNSWALFRKMAFLNKEEELENSNLVRIG 360
A GS+IL+TTRNLQ AQASDTV FHHLKELD ++SWALFRKMAFLN+EEE+ENSN VRIG
Sbjct: 327 AKGSRILITTRNLQVAQASDTVQFHHLKELDNESSWALFRKMAFLNEEEEIENSNKVRIG 386
Query: 361 KEIVAKLKGYPLSIRVVGRLLYFKNTEMDWSSFKDNELDSILQEDDQIQPILKISFNHLP 420
KEI+AKLKG PL+IR+VGRLLYFKNTEMDW SFKDN+L +ILQ+++QIQPILKISFNHLP
Sbjct: 387 KEIIAKLKGSPLTIRIVGRLLYFKNTEMDWLSFKDNDLGTILQQENQIQPILKISFNHLP 446
Query: 421 PKLKQCFTYCALFPKDYEFKKNGLVKQWMAQGFIQAHNKKAIEDVGDDYFQELVGRSFFQ 480
LK CFTYCALFPKDYEF+K+GLVKQWMAQGFIQ+H+ K IEDVGDDYF+EL+GRSFF
Sbjct: 447 SNLKHCFTYCALFPKDYEFQKDGLVKQWMAQGFIQSHSNKEIEDVGDDYFKELLGRSFFH 506
Query: 481 DIRKNKWGDLKYCKMHDLLHDLACSIGENECVVVSDDVGSIDKRTRHASFLLS-KRLTRE 540
+++ NKWGD+K CKMHDL+HDLAC I ENECV SD SIDKRTRH SF + R + E
Sbjct: 507 NVKVNKWGDVKECKMHDLIHDLACWIVENECVDASDKTKSIDKRTRHVSFPSNYSRKSWE 566
Query: 541 VVSKSSIEVTSLRTLDIDSRASFRSFKKTCHMNLFQLRTLNLDRCCCHP-PKFVDKLKHL 600
+ +KS EV +LRTL N +LR+LNL PKF+ +L+HL
Sbjct: 567 LEAKSLTEVKNLRTL--------HGPPFLLSENHLRLRSLNLGYSKFQKIPKFISQLRHL 626
Query: 601 RYLNLSGLNVTFLPNSITTLYNLETLILRYCLWLRKLPKDINNLINLRHLDIYDCSSLTH 660
RYL++S ++ FLP IT LYNLETLILR+C LR+LP DINNLINL+HLD++ C LTH
Sbjct: 627 RYLDISDHDMKFLPKFITKLYNLETLILRHCSDLRELPTDINNLINLKHLDVHGCYRLTH 686
Query: 661 MPKGLGGMTSLQTMSMFVLGKNKGGDLSALNGLKSLRGLLCIKGLQFCTTADLKN----- 720
MPKGLGG+TSLQTM++FVLGK+KG DLS LN L LRG L IKGL+ CTT DLKN
Sbjct: 687 MPKGLGGLTSLQTMNLFVLGKDKGCDLSELNELARLRGSLLIKGLELCTTTDLKNAKYME 746
Query: 721 -------LELHWDIKMDHEDALDDGDNDDEGVLEGLKPHSNIRKMIIKGYRGMKLCDWFS 780
L+L W+ + + +NDDE VL+ LKPHSN+ KM I+GYRG+KLC+W S
Sbjct: 747 EKFGIQKLKLRWNRDLYDAETDYASENDDERVLDCLKPHSNVHKMQIRGYRGVKLCNWLS 806
Query: 781 SNFLGGLVSIELSHCEKLEHLPQFDQFLYLKHLLLGYLPNIEYIDSGNSVSSSTTFFPSL 840
++LGGLV+IEL CEKL+HLPQFDQF +LKHLLL LP+IEYID+ NS+SSS TFFPSL
Sbjct: 807 FDYLGGLVNIELQSCEKLQHLPQFDQFPFLKHLLLENLPSIEYIDNNNSLSSS-TFFPSL 866
Query: 841 EKLRIESMPKLKGWWKGEIS---------FPTTILHQLSELCIFYCPLLASIPQHPSLES 900
EKL I +MP LKGWWKGE FP TILH LS L I CP LASIPQHP L S
Sbjct: 867 EKLTIMTMPNLKGWWKGETPPESARYSALFP-TILHHLSRLDISNCPQLASIPQHPPLRS 926
Query: 901 LRICGVSVQLFQMVIRMATDLSEHSSSSSTLSKLSFLEIGTIDLEFLPVELFCNMTHLES 960
L + VSVQLF MVI+MAT + + SSS LSKLS L I IDLEFLP ELF + T LE
Sbjct: 927 LALNDVSVQLFDMVIKMAT--TPAADSSSALSKLSILHIQNIDLEFLPEELFGSTTDLEI 986
Query: 961 LIIERCKSLQMSSPHPVDEDND-VVWKKLSNLRTLRLESILKLEYFPKSLKYITSLETLK 1020
+ CK+LQMSS H VDEDND V+ KKL NL +L + + +LEY K LKY+T+LE L
Sbjct: 987 FTVVNCKNLQMSSSHLVDEDNDGVLGKKLGNLHSLGIFDMPQLEYLWKELKYMTTLERLD 1046
Query: 1021 LSNCENLVSTEGIGELISLSHLEIDRCPNLPILSEDVGDLISLSHLLIWNCPKLTSLSEG 1080
L NC N+VS EGI L SLS L I C NL L E + L SLS+L I CP LTSL G
Sbjct: 1047 LYNCPNIVSLEGISHLTSLSSLRICNCSNLTSLPEGISHLTSLSYLTIVCCPNLTSLPAG 1106
Query: 1081 ITRLTSLSSLCLEDCPNLVSLPQEFLHHHSSLPGGRFLRILNCPKL 1103
I LTSLS+L ++ C NL SLP E + H +SL I CP L
Sbjct: 1107 IGHLTSLSTLLIKYCVNLTSLP-EGVSHLTSLSS---FTIEECPCL 1135
BLAST of Cucsa.128100 vs. NCBI nr
Match:
gi|778728520|ref|XP_011659433.1| (PREDICTED: putative disease resistance protein RGA4 [Cucumis sativus])
HSP 1 Score: 103.2 bits (256), Expect = 3.0e-18
Identity = 80/204 (39.22%), Postives = 102/204 (50.00%), Query Frame = 1
Query: 906 STLSKLSFLEIGTIDLEFLPVELFCNMTHLESLIIERCKSLQMSSPHPVDEDNDVVWKKL 965
+TL +L I + +E ++T L SL I C +L S P + L
Sbjct: 1027 TTLERLDLYNCPNI----VSLEGISHLTSLSSLRICNCSNLT-SLPEGISH--------L 1086
Query: 966 SNLRTLRLESILKLEYFPKSLKYITSLETLKLSNCENLVST-EGIGELISLSHLEIDRCP 1025
++L L + L P + ++TSL TL + C NL S EG+ L SLS I+ CP
Sbjct: 1087 TSLSYLTIVCCPNLTSLPAGIGHLTSLSTLLIKYCVNLTSLPEGVSHLTSLSSFTIEECP 1146
Query: 1026 NLPILSEDVGDLISLSHLLIWNCPKLTSLSEGITRLTSLSSLCLEDCPNLVSLPQEFLHH 1085
L L E V L SLS L+I C LTSL EGI LTSLS +E+C NL SLP+ L H
Sbjct: 1147 CLTSLPEGVSHLTSLSTLIIRRCVNLTSLPEGIGHLTSLSIFTIEECLNLTSLPEGLL-H 1206
Query: 1086 HSSLPGGRFLRILNCPKLQIQDKK 1109
SSL G L + CPKL KK
Sbjct: 1207 LSSLRGS--LTVSKCPKLSKTWKK 1214
HSP 2 Score: 50.4 bits (119), Expect = 2.3e-02
Identity = 34/103 (33.01%), Postives = 57/103 (55.34%), Query Frame = 1
Query: 572 NLFQLRTLNLDRCCCHPPKFVDKLKHLRYLNLSGLN----VTFLPNSITTLYNLETLILR 631
+L L TL L + C + + + HL L+ + +T LP ++ L +L TLI+R
Sbjct: 1096 HLTSLSTL-LIKYCVNLTSLPEGVSHLTSLSSFTIEECPCLTSLPEGVSHLTSLSTLIIR 1155
Query: 632 YCLWLRKLPKDINNLINLRHLDIYDCSSLTHMPKGLGGMTSLQ 671
C+ L LP+ I +L +L I +C +LT +P+GL ++SL+
Sbjct: 1156 RCVNLTSLPEGIGHLTSLSIFTIEECLNLTSLPEGLLHLSSLR 1197
HSP 3 Score: 1202.6 bits (3110), Expect = 0.0e+00
Identity = 648/1087 (59.61%), Postives = 802/1087 (73.78%), Query Frame = 1
Query: 1 MADSVLFNVAASVITKLGSSALRELGSLW-GVNDELDKLQNTLSAIKAVLLDAEEQQSKS 60
MA+S+LF +AA++ TKLGS L ELG LW G ++ELDKL++TLSAI+AVLLDAEE+Q KS
Sbjct: 1 MAESILFTLAANIATKLGSFPLHELGLLWTGFHEELDKLKDTLSAIQAVLLDAEEKQYKS 60
Query: 61 HTVKDWIAKIKDVFYDIDDLIDEFSYETLRRQVLTKDRTITKQVRIFFSKSNQIAFGFKM 120
+ VK+W++++KD FYDIDDL+DEFSYE+++RQV+ K RT KQVRIFFS+SNQIAF KM
Sbjct: 61 YAVKEWVSRLKDAFYDIDDLMDEFSYESIKRQVMIKHRTNNKQVRIFFSESNQIAFRLKM 120
Query: 121 GQTIKKVREKLDAIAAIKAQLHLSVCAREVRDNEPRKVRETSSFIPEGEIIGRDEDRKSV 180
G IK+VREKLD +A KAQ +LS RE+R++E K ETSSFI EGE+IGRD+D+K +
Sbjct: 121 GYKIKRVREKLDIVAIDKAQFNLSEYTREIRNDETTKRPETSSFILEGEVIGRDDDKKGI 180
Query: 181 MDFLLNTSNITKDNVEVVSIVGMGGLGKTALAQTVYNDEKINNRFKWKIWVCISQEFDIK 240
+ FLL+T N+ ++NV VV+I+GMGGLGKTALAQ++Y D K N F+ +WVCIS+EFD+K
Sbjct: 181 VHFLLDT-NVAQENVAVVAIIGMGGLGKTALAQSIYGDMKENKHFELTMWVCISEEFDVK 240
Query: 241 VIVEKILESITKTKQE-SLQLDILQSMLQEKIYGKKYLLVMDDVWNVDHEKWIGLKRFLM 300
+IVEKI+ES+TK + E +LQLD LQ+ML+EKI GK+YLLVMDDVWNV+ EKWI LK FL
Sbjct: 241 IIVEKIIESLTKKRPEPNLQLDTLQNMLREKIDGKRYLLVMDDVWNVNREKWINLKAFLT 300
Query: 301 GGASGSKILVTTRNLQTAQASDTVWFHHLKELDKDNSWALFRKMAFLNKEEELENSNLVR 360
GGA GS+IL+TTR Q A S+TV FHHL EL+K++SW LFRKMAF N+ E LENS LV
Sbjct: 301 GGAKGSRILITTRTHQVAHTSETVLFHHLSELNKNSSWELFRKMAFSNESEVLENSKLVI 360
Query: 361 IGKEIVAKLKGYPLSIRVVGRLLYFKNTEMDWSSFKDNELDSILQEDDQIQPILKISFNH 420
IGKEIVA LKG PL++RV+G LY K TE DW SFKDNEL +I+Q++++IQ ILKISFN
Sbjct: 361 IGKEIVANLKGSPLALRVIGSYLYSKKTEKDWLSFKDNELGTIMQQENEIQSILKISFNQ 420
Query: 421 LPPKLKQCFTYCALFPKDYEFKKNGLVKQWMAQGFIQAHNKKAIEDVGDDYFQELVGRSF 480
L LKQCFTYCALFPKDYE +K+ L+KQWMAQGFIQ NKK +EDVGDDYF+EL+GRSF
Sbjct: 421 LSSGLKQCFTYCALFPKDYEIQKDDLIKQWMAQGFIQPQNKKTMEDVGDDYFKELLGRSF 480
Query: 481 FQDIRKNKWGDLKYCKMHDLLHDLACSIGENECVVVSDDVGSIDKRTRHASFLLSKRLTR 540
FQDI+KNKWG++K K+HD +HDLACS+ EN CV+ +DD +IDKRTRH S TR
Sbjct: 481 FQDIKKNKWGEIKEFKIHDFMHDLACSVVENVCVLTNDDTKTIDKRTRHVSISTFISKTR 540
Query: 541 -EVVSKSSIEVTSLRTLDIDSRASFRSFKKTCHMNLFQLRTLNLDRCCCHPPKFVDKLKH 600
+V+++S E +LRTL+ + K N QLRTLN+D H PK + K+KH
Sbjct: 541 WKVITESLKEAKNLRTLNY-------ACDKIDLSNHLQLRTLNVD-FLYHVPKCIGKMKH 600
Query: 601 LRYLNLSGLNVTFLPNSITTLYNLETLILRYCLWLRKLPKDINNLINLRHLDIYDCSS-- 660
LRY+NLS + FLP IT LYNLETLILR C L LP DI NLINLRHLDI C+S
Sbjct: 601 LRYVNLSNTWIDFLPKFITKLYNLETLILRNCQRLMGLPSDIKNLINLRHLDIMHCNSNC 660
Query: 661 LTHMPKGLGGMTSLQTMSMFVLGKNKGGDLSALNGLKSLRGLLCIKGLQFCTTADLKN-- 720
+ MP GLG MTSLQTM++FVLG GG+LS LNGL SLRG L IK LQFC T +L+N
Sbjct: 661 WSSMPMGLGSMTSLQTMNLFVLGAKWGGELSELNGLNSLRGSLRIKQLQFCITPNLENAK 720
Query: 721 ----------LELHWDIKMDHEDALDDGDNDDEGVLEGLKPHSNIRKMIIKGYRGMKLCD 780
LELHWD + + +++DE VLE LKPH N++K+ I+GY+G+KLC+
Sbjct: 721 YLEEKSGIQKLELHWD--SSYGIGRNTFNDEDEKVLECLKPHPNLQKIRIEGYKGVKLCN 780
Query: 781 WFSSNFLGGLVSIELSHCEKLEHLPQFDQFLYLKHLLLGYLPNIEYIDSGNSVSSS-TTF 840
WFS +G LV+I+L CEKL+HLPQFDQF +LKHL L LPNIE+ID N VSSS TTF
Sbjct: 781 WFSFGSIGSLVTIKLCECEKLKHLPQFDQFPFLKHLHLEDLPNIEFIDDNNYVSSSLTTF 840
Query: 841 FPSLEKLRIESMPKLKGWWKGEI-----SFPTTILHQLSELCIFYCPLLASIPQHPSLES 900
FPSLEKL I +PKLK WWKGE FPTT LH LS L I +CP L SIPQH L S
Sbjct: 841 FPSLEKLSIIQLPKLKEWWKGEFIDQTTPFPTT-LHHLSRLKINHCPQLGSIPQHGPLRS 900
Query: 901 LRICGVSVQLFQMVIRMATD--LSEHSSSSSTLSKLSFLEIGTIDLEFLPV-ELFCNMTH 960
L + GVS+QLF++V+ MAT + SSSST LS L I +D EFL + +LF NMTH
Sbjct: 901 LDVSGVSLQLFELVMEMATTNIIVGQDSSSSTTRSLSSLSIWDMDFEFLQLHDLFSNMTH 960
Query: 961 LESLIIERCKSLQMSSPHPVDEDNDVVWKKLSNLRTLRLESILKLEYFPKSLKYITSLET 1020
L+SL+I CK+++MSS + V+WK+L +LR L L SI +LE PK L+Y+T+LE
Sbjct: 961 LKSLVIGNCKNIKMSSSR-----DGVIWKELGSLRRLDLCSIPELECLPKGLQYVTTLEC 1020
Query: 1021 LKLSNCENLVSTEGIGELISLSHLEIDRCPNLPILSEDVGDLISLSHLLIWNCPKLTSLS 1062
LKL +C NLVS EGI L SLS LEI+ CPNL +++G L SLS L I CP L SL
Sbjct: 1021 LKLYDCRNLVSIEGIEHLTSLSLLEIEYCPNLISYPQEMGQLTSLSRLRINGCPNLPSLP 1070
The following BLAST results are available for this feature:
Match Name | E-value | Identity | Description | |
RGA4_SOLBU | 3.1e-140 | 34.38 | Putative disease resistance protein RGA4 OS=Solanum bulbocastanum GN=RGA4 PE=2 S... | [more] |
RGA1_SOLBU | 1.2e-139 | 34.34 | Putative disease resistance protein RGA1 OS=Solanum bulbocastanum GN=RGA1 PE=2 S... | [more] |
RGA2_SOLBU | 5.0e-138 | 34.12 | Disease resistance protein RGA2 OS=Solanum bulbocastanum GN=RGA2 PE=1 SV=1 | [more] |
RGA3_SOLBU | 5.0e-138 | 32.93 | Putative disease resistance protein RGA3 OS=Solanum bulbocastanum GN=RGA3 PE=2 S... | [more] |
R13L1_ARATH | 4.0e-119 | 31.16 | Putative disease resistance RPP13-like protein 1 OS=Arabidopsis thaliana GN=RPPL... | [more] |
Match Name | E-value | Identity | Description | |
A0A0A0K9P7_CUCSA | 0.0e+00 | 98.49 | Uncharacterized protein OS=Cucumis sativus GN=Csa_7G425940 PE=3 SV=1 | [more] |
A0A0A0K9P7_CUCSA | 3.1e-86 | 88.38 | Uncharacterized protein OS=Cucumis sativus GN=Csa_7G425940 PE=3 SV=1 | [more] |
A0A0A0K5Z6_CUCSA | 2.1e-18 | 39.22 | Uncharacterized protein OS=Cucumis sativus GN=Csa_7G425920 PE=4 SV=1 | [more] |
A0A0D2NDD9_GOSRA | 7.8e-207 | 38.66 | Uncharacterized protein OS=Gossypium raimondii GN=B456_005G155200 PE=3 SV=1 | [more] |
A0A0A0KIT2_CUCSA | 6.6e-206 | 54.28 | Uncharacterized protein OS=Cucumis sativus GN=Csa_6G375730 PE=3 SV=1 | [more] |
Match Name | E-value | Identity | Description | |
AT3G14470.1 | 2.2e-120 | 31.16 | NB-ARC domain-containing disease resistance protein | [more] |
AT3G14460.1 | 1.0e-101 | 30.79 | LRR and NB-ARC domains-containing disease resistance protein | [more] |
AT1G58602.1 | 1.6e-70 | 25.70 | LRR and NB-ARC domains-containing disease resistance protein | [more] |
AT3G50950.2 | 8.0e-70 | 27.55 | HOPZ-ACTIVATED RESISTANCE 1 | [more] |
AT3G46730.1 | 7.5e-68 | 29.06 | NB-ARC domain-containing disease resistance protein | [more] |