Cucsa.126340 (gene) Cucumber (Gy14) v1

Overview
NameCucsa.126340
Typegene
OrganismCucumis sativus (Cucumber (Gy14) v1)
DescriptionBifunctional nuclease
Locationscaffold01017 : 254300 .. 256959 (+)
Genome Viewer
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGGAAAATTGGTATTTTTGGTGGTGTTAATTTTTATTTCATTCCTTCTAGTCCTGCCTTGTGCTCAAGGATGGAGCAAAGAAGGTCATATCCTAACATGTGAAATTGCACAGGTAACTTTATTTACTTTTTCTTGTCTGATGATCTTTTAATTAAATTGGACGTTTTGGATCAAATTCTGCCTTGACCATTTGAACTCAACACATAGGGTCATTTTTACTCTTAAATTTGACAAACTGTCTCCATTTTTATCTGCAAATTTCTATTTTCTTTAATCAGTTTTTATATAAGCTTAAAAAAAaTTGTTATATGGGTGGGATCTGAGGAGTTTGTTATGAAATCAACAAATAAAACAAGCATAAAAACGTAAATGAGGAGAGAATCGACATAGAAGCATATTAGCTACATTTATGCTCATTAGAAAGAATATTTCAGATTACAGAACAAAGACGCTTCTATAAATGGGGAGTTCATACAACCAATTTGATCTTCAAATCTTAAGATTAAAATGGTAAACAACGTAAATACAGTGAGATCTTAAACATTTTTGTTTAATTAGTTGTTGAGAGGTGAGATATCAGATCTAAATGTATTCACCTCAGTTATAAAATATATATCAGGCAAGAAACTGATGTGTTTTGAAAGAGAAATTAACAACAAAAaCAAATTTTGGGCTGAATTTTGGAAGATTTATCAGACAATTGACATTGTAAATTGATGTAGCTCAATTCGATCATGTCAAACTTTTTTTTtGTtGAAAGCAGCGCGAGTCTTAAAAAGGaGGaTTTTACATGTTAAACAAaTTTAGAAACAAAAaTTTTAAAaTTtGAGACACACAATTGAATATTGAAGAGGACAACTAATGGGTAAGTTTAGATTATAATCCAACCACATTCAGGAGCTACTGATTCCAGAGGCAGCAGAGGCAGTTCAAGATCTGTTACCTGAAAGTGCCGGAGGAAATCTATCGGCTATGTGCGTATGGCCGGACCAAATCCGACTCCAGTCTAAGTACCGCTGGGCCAGTCCCCTTCACTACGCCAACACCCCCGACTCTTGTTCTTTCGTCTACAAAAGTAAAGTAACACCCTTTTATTCTCCCATCCCTAAGGCTTTCGAATTATTAAACTAAACGGTGCGTTTTTGTTTAGGGGATTGTCATAATGACGCCGGACAGCCGGACATGTGCGTCGCCGGTGCCATTCGAAATTTCACCACTCAGCTCACAACCTACCGAACACAAGGCTTCGACAGTCCTCGTAACTGACCCGACCCGACCCACTCGAATTATTCCTGTGATAATATGAAAATTATTTAATTTCATTTTTATTATTCTAGATAACTTGACCGAGGCCTTGCTCTTTCTATCGCATTTCGTTGGGGATATTCATCAGGTAATACTAAAGTTATTCAAAAACGTTTATGTGTTGAATTGCTTTAATTTAATTATGAGATATGTAATTATAACTTATTTTACATATACATATATATACCTATAATTTCAGCCATTGCATGTGGGGTTCGAGAGTGATGCGGGAGGAAACACCATAGAAGTACGATGGTTCCGTCGTAAATCAAACCTCCATCACGTAAGATCTTAATCTAAAAACATACCAAAACAATATGAATCTATATGTTTTAAGTTTTATGAATTCTATTACATTTAGATTATAAATTACTAATTATATGGAATAATGCAGGTGTGGGATAGAGACATTATTCTTGAAGCTCTAGGAGATTATTACGACAAAGACGGTGGCCTTCTCTTAGATGAACTTAACCGGAATTTAACTCAGGTAATTAATTAATACTTTTTATTTTTtCTTTATTACATTAGTACATTAGTTTATAATGTTAGGATATTAGAAGGTTTACTATCTTCTTATCAAAATTCTGTTTTTGTAGGGAATTTGGAGCAATGACGTTTCGGAATGGGAGCGTTGTTCTACTGTTAATTCATGTGTTAATAGGTATTtATAGAAAATGAAATTAGGTTATATTTTGTAAATATTTTTTtGTTTTTTTTtAACCATAGTAACAAACTTGTGGATTAGGTGGGCTGATGAGAGTACAGGGTTGGCTTGCAAGTGGGCATATGAAGGAGTTGAAGCTGGTATTACTTTATCAGGTAAACCCAATCAATCAATCAATCAAACATTGTTTAAATTAATATCATAGTCATTAATTCCTAAAATTATTTGAAATATAACATATGATGAGAGGATAATGAAATCCGTAACTGATGTGATTTGTGTACAGAGGAATACTACGATTCAAGGTTGCCAATTGTGATGGAACGATTAGCTCAAGGTGGGGTCCGGTTGGCAATGCTCTTGAACCGGGTTTTTGCTGAAGATGCTACACGAGGATTTGCCTATTCATCTTGATTAATTCCACCATCAAATTTAGGATTTCTTTTTCTTCTTTCTTCTAACTTAATTATGTCAGGAAAATATCTTAGATTTCAAAGGTTACTAGAAATGAAAGCAAATTGTAGTGTTGAATAAATTGGCATTTTAGTTCATTTTACAAAATTTCATCAACAAATTCACATGCTCTTTACATAGATGTAAATGAATTAATCAAAGTAATAATGCAGTACATCTAAATTGGTATACTACATGATTTGTAACAAATAACATTTGATCAAATGTAACCC

mRNA sequence

ATGGGAAAATTGGTATTTTTGGTGGTGTTAATTTTTATTTCATTCCTTCTAGTCCTGCCTTGTGCTCAAGGATGGAGCAAAGAAGGTCATATCCTAACATGTGAAATTGCACAGGAGCTACTGATTCCAGAGGCAGCAGAGGCAGTTCAAGATCTGTTACCTGAAAGTGCCGGAGGAAATCTATCGGCTATGTGCGTATGGCCGGACCAAATCCGACTCCAGTCTAAGTACCGCTGGGCCAGTCCCCTTCACTACGCCAACACCCCCGACTCTTGTTCTTTCGTCTACAAAAGGGATTGTCATAATGACGCCGGACAGCCGGACATGTGCGTCGCCGGTGCCATTCGAAATTTCACCACTCAGCTCACAACCTACCGAACACAAGGCTTCGACAGTCCTCATAACTTGACCGAGGCCTTGCTCTTTCTATCGCATTTCGTTGGGGATATTCATCAGCCATTGCATGTGGGGTTCGAGAGTGATGCGGGAGGAAACACCATAGAAGTACGATGGTTCCGTCGTAAATCAAACCTCCATCACGTGTGGGATAGAGACATTATTCTTGAAGCTCTAGGAGATTATTACGACAAAGACGGTGGCCTTCTCTTAGATGAACTTAACCGGAATTTAACTCAGGGAATTTGGAGCAATGACGTTTCGGAATGGGAGCGTTGTTCTACTGTTAATTCATGTGTTAATAGGTGGGCTGATGAGAGTACAGGGTTGGCTTGCAAGTGGGCATATGAAGGAGTTGAAGCTGGTATTACTTTATCAGAGGAATACTACGATTCAAGGTTGCCAATTGTGATGGAACGATTAGCTCAAGGTGGGGTCCGGTTGGCAATGCTCTTGAACCGGGTTTTTGCTGAAGATGCTACACGAGGATTTGCCTATTCATCTTGATTAATTCCACCATCAAATTTAGGATTTCTTTTTCTTCTTTCTTCTAACTTAATTATGTCAGGAAAATATCTTAGATTTCAAAGGTTACTAGAAATGAAAGCAAATTGTAGTGTTGAATAAATTGGCATTTTAGTTCATTTTACAAAATTTCATCAACAAATTCACATGCTCTTTACATAGATGTAAATGAATTAATCAAAGTAATAATGCAGTACATCTAAATTGGTATACTACATGATTTGTAACAAATAACATTTGATCAAATGTAACCC

Coding sequence (CDS)

ATGGGAAAATTGGTATTTTTGGTGGTGTTAATTTTTATTTCATTCCTTCTAGTCCTGCCTTGTGCTCAAGGATGGAGCAAAGAAGGTCATATCCTAACATGTGAAATTGCACAGGAGCTACTGATTCCAGAGGCAGCAGAGGCAGTTCAAGATCTGTTACCTGAAAGTGCCGGAGGAAATCTATCGGCTATGTGCGTATGGCCGGACCAAATCCGACTCCAGTCTAAGTACCGCTGGGCCAGTCCCCTTCACTACGCCAACACCCCCGACTCTTGTTCTTTCGTCTACAAAAGGGATTGTCATAATGACGCCGGACAGCCGGACATGTGCGTCGCCGGTGCCATTCGAAATTTCACCACTCAGCTCACAACCTACCGAACACAAGGCTTCGACAGTCCTCATAACTTGACCGAGGCCTTGCTCTTTCTATCGCATTTCGTTGGGGATATTCATCAGCCATTGCATGTGGGGTTCGAGAGTGATGCGGGAGGAAACACCATAGAAGTACGATGGTTCCGTCGTAAATCAAACCTCCATCACGTGTGGGATAGAGACATTATTCTTGAAGCTCTAGGAGATTATTACGACAAAGACGGTGGCCTTCTCTTAGATGAACTTAACCGGAATTTAACTCAGGGAATTTGGAGCAATGACGTTTCGGAATGGGAGCGTTGTTCTACTGTTAATTCATGTGTTAATAGGTGGGCTGATGAGAGTACAGGGTTGGCTTGCAAGTGGGCATATGAAGGAGTTGAAGCTGGTATTACTTTATCAGAGGAATACTACGATTCAAGGTTGCCAATTGTGATGGAACGATTAGCTCAAGGTGGGGTCCGGTTGGCAATGCTCTTGAACCGGGTTTTTGCTGAAGATGCTACACGAGGATTTGCCTATTCATCTTGA

Protein sequence

MGKLVFLVVLIFISFLLVLPCAQGWSKEGHILTCEIAQELLIPEAAEAVQDLLPESAGGNLSAMCVWPDQIRLQSKYRWASPLHYANTPDSCSFVYKRDCHNDAGQPDMCVAGAIRNFTTQLTTYRTQGFDSPHNLTEALLFLSHFVGDIHQPLHVGFESDAGGNTIEVRWFRRKSNLHHVWDRDIILEALGDYYDKDGGLLLDELNRNLTQGIWSNDVSEWERCSTVNSCVNRWADESTGLACKWAYEGVEAGITLSEEYYDSRLPIVMERLAQGGVRLAMLLNRVFAEDATRGFAYSS*
Homology
[Close]
BLAST of Cucsa.126340 vs. Swiss-Prot
Match: ENDO1_ARATH (Endonuclease 1 OS=Arabidopsis thaliana GN=ENDO1 PE=1 SV=1)

HSP 1 Score: 359.8 bits (922), Expect = 3.0e-98
Identity = 167/286 (58.39%), Postives = 219/286 (76.57%), Query Frame = 1 

Query: 7   LVVLIFISFLLVLPCAQGWSKEGHILTCEIAQELLIPEAAEAVQDLLPESAGGNLSAMCV 66
           L++++ I  L  +   + WSKEGHILTC IAQ LL    A  V++LLP+   G+LSA+CV
Sbjct: 11  LILVLGILILCSVSSVRSWSKEGHILTCRIAQNLLEAGPAHVVENLLPDYVKGDLSALCV 70

Query: 67  WPDQIRLQSKYRWASPLHYANTPD-SCSFVYKRDCHNDAGQPDMCVAGAIRNFTTQLTTY 126
           WPDQIR   KYRW S LHY +TPD +CS+ Y RDCH+  G  DMCV GAI+NFT+QL  Y
Sbjct: 71  WPDQIRHWYKYRWTSHLHYIDTPDQACSYEYSRDCHDQHGLKDMCVDGAIQNFTSQLQHY 130

Query: 127 RTQGFDSPHNLTEALLFLSHFVGDIHQPLHVGFESDAGGNTIEVRWFRRKSNLHHVWDRD 186
                D  +N+TEALLFLSHF+GDIHQP+HVGF SD GGNTI++RW++ KSNLHHVWDR+
Sbjct: 131 GEGTSDRRYNMTEALLFLSHFMGDIHQPMHVGFTSDEGGNTIDLRWYKHKSNLHHVWDRE 190

Query: 187 IILEALGDYYDKDGGLLLDELNRNLTQGIWSNDVSEWERCSTVNSCVNRWADESTGLACK 246
           IIL AL + YDK+  LL ++L +N+T G+W +D+S W  C+ + +C +++A ES  LACK
Sbjct: 191 IILTALKENYDKNLDLLQEDLEKNITNGLWHDDLSSWTECNDLIACPHKYASESIKLACK 250

Query: 247 WAYEGVEAGITLSEEYYDSRLPIVMERLAQGGVRLAMLLNRVFAED 292
           W Y+GV++G TLSEEY+++RLPIVM+R+ QGGVRLAM+LNRVF++D
Sbjct: 251 WGYKGVKSGETLSEEYFNTRLPIVMKRIVQGGVRLAMILNRVFSDD 296

[Close]
BLAST of Cucsa.126340 vs. Swiss-Prot
Match: ENDO2_ARATH (Endonuclease 2 OS=Arabidopsis thaliana GN=ENDO2 PE=1 SV=1)

HSP 1 Score: 313.5 bits (802), Expect = 2.4e-84
Identity = 149/283 (52.65%), Postives = 201/283 (71.02%), Query Frame = 1 

Query: 7   LVVLIFISFLLVLPCAQGWSKEGHILTCEIAQELLIPEAAEAVQDLLPESAGGNLSAMCV 66
           ++++I +  L   P   GW KEGH + C+IAQ  L   AA+AV++LLPESA G+LS++C+
Sbjct: 10  VMMIITVWLLYAAPNIHGWGKEGHEIICKIAQTRLDETAAKAVKELLPESAEGDLSSLCL 69

Query: 67  WPDQIRLQSKYRWASPLHYANTPDSCSFVYKRDCHNDAGQPDMCVAGAIRNFTTQLTTYR 126
           W D+++   +Y W+SPLHY NTPD+CS+ Y RDC +++G+   CVAGAI N+TTQL +Y+
Sbjct: 70  WADRVKF--RYHWSSPLHYINTPDACSYQYNRDCKDESGEKGRCVAGAIYNYTTQLLSYK 129

Query: 127 TQGFD-SPHNLTEALLFLSHFVGDIHQPLHVGFESDAGGNTIEVRWFRRKSNLHHVWDRD 186
           T     S +NLTEALLF+SHF+GDIHQPLHV + SD GGNTIEV W+ RK+NLHH+WD +
Sbjct: 130 TAASSQSQYNLTEALLFVSHFMGDIHQPLHVSYASDKGGNTIEVHWYTRKANLHHIWDSN 189

Query: 187 IILEALGDYYDKDGGLLLDELNRNLTQGIWSNDVSEWERCSTVNSCVNRWADESTGLACK 246
           II  A  D Y+     ++D L +N+T   W++ V  WE C+   +C + +A E    AC 
Sbjct: 190 IIETAEADLYNSALEGMVDALKKNITTE-WADQVKRWETCTKKTACPDIYASEGIQAACD 249

Query: 247 WAYEGVEAGITLSEEYYDSRLPIVMERLAQGGVRLAMLLNRVF 289
           WAY+GV  G TL +EY+ SRLPIV +RLAQGGVRLA  LNR+F
Sbjct: 250 WAYKGVTEGDTLEDEYFYSRLPIVYQRLAQGGVRLAATLNRIF 289

[Close]
BLAST of Cucsa.126340 vs. Swiss-Prot
Match: ENDO4_ARATH (Endonuclease 4 OS=Arabidopsis thaliana GN=ENDO4 PE=3 SV=1)

HSP 1 Score: 292.7 bits (748), Expect = 4.5e-78
Identity = 145/268 (54.10%), Postives = 181/268 (67.54%), Query Frame = 1 

Query: 25  WSKEGHILTCEIAQELLIPEAAEAVQDLLPESAGGNLSAMCVWPDQIRLQSKYRWASPLH 84
           W KEGH   C+IA+     E   AV+ LLP+SA G+L+++C WPD+I+   ++RW SPLH
Sbjct: 25  WGKEGHYTVCKIAESYFEEETVAAVKKLLPKSADGDLASVCSWPDEIKHHWQWRWTSPLH 84

Query: 85  YANTPD-SCSFVYKRDCHNDAGQPDMCVAGAIRNFTTQLTTYRTQGFDSPH-NLTEALLF 144
           Y +TPD  C++ Y RDCH+     D CV GAI N+T QL +         H NLTEAL+F
Sbjct: 85  YVDTPDYRCNYEYCRDCHDTHKNQDRCVTGAIFNYTMQLMSASENSDTIVHYNLTEALMF 144

Query: 145 LSHFVGDIHQPLHVGFESDAGGNTIEVRWFRRKSNLHHVWDRDIILEALGDYYDKDGGLL 204
           LSHF+GDIHQPLHVGF  D GGNTI VRW+RRK+NLHHVWD  II  AL  YY+K   L+
Sbjct: 145 LSHFIGDIHQPLHVGFLGDEGGNTITVRWYRRKTNLHHVWDNMIIESALKTYYNKSLPLM 204

Query: 205 LDELNRNLTQGIWSNDVSEWERCS-TVNSCVNRWADESTGLACKWAYEGVEAGITLSEEY 264
           ++ L  NLT   WSNDV  WE C     +C N +A ES  LACK+AY     G TL ++Y
Sbjct: 205 IEALQANLTND-WSNDVPLWESCQLNQTACPNPYASESINLACKYAYRNATPGTTLGDDY 264

Query: 265 YDSRLPIVMERLAQGGVRLAMLLNRVFA 290
           + SRLPIV +RLAQGG+RLA  LNR+F+
Sbjct: 265 FLSRLPIVEKRLAQGGIRLAATLNRIFS 291

[Close]
BLAST of Cucsa.126340 vs. Swiss-Prot
Match: ENDO5_ARATH (Endonuclease 5 OS=Arabidopsis thaliana GN=ENDO5 PE=2 SV=1)

HSP 1 Score: 267.3 bits (682), Expect = 2.0e-70
Identity = 138/287 (48.08%), Postives = 178/287 (62.02%), Query Frame = 1 

Query: 10  LIFISFLLVLPCAQG---WSKEGHILTCEIAQELLIPEAAEAVQDLLPESA-GGNLSAMC 69
           L  +S L++     G   W K+GH   C++A+     +   AV+ LLPES  GG L+  C
Sbjct: 3   LWIVSVLVLTHLVHGALCWGKDGHYTVCKLAEGFFEDDTIAAVKKLLPESVDGGGLADFC 62

Query: 70  VWPDQIRLQSKYRWASPLHYANTPD-SCSFVYKRDCHNDAGQPDMCVAGAIRNFTTQLTT 129
            WPD+I+  S+++W S LHY NTP+  C++ Y RDCH+     D CV GAI N+T QL +
Sbjct: 63  SWPDEIKKLSQWQWTSTLHYVNTPEYRCNYEYCRDCHDTHKHKDWCVTGAIFNYTNQLMS 122

Query: 130 YRTQGFDSPH-NLTEALLFLSHFVGDIHQPLHVGFESDAGGNTIEVRWFRRKSNLHHVWD 189
                 +  H NLTEALLFLSH++GD+HQPLH GF  D GGNTI V W+  KSNLHHVWD
Sbjct: 123 ASENSQNIVHYNLTEALLFLSHYMGDVHQPLHTGFLGDLGGNTIIVNWYHNKSNLHHVWD 182

Query: 190 RDIILEALGDYYDKDGGLLLDELNRNLTQGIWSNDVSEWERCS-TVNSCVNRWADESTGL 249
             II  AL  YY+     ++  L   L  G WSNDV  W+ C     +C N +A ES  L
Sbjct: 183 NMIIDSALETYYNSSLPHMIQALQAKLKNG-WSNDVPSWKSCHFHQKACPNLYASESIDL 242

Query: 250 ACKWAYEGVEAGITLSEEYYDSRLPIVMERLAQGGVRLAMLLNRVFA 290
           ACK+AY     G TL +EY+ SRLP+V +RLAQGG+RLA  LNR+F+
Sbjct: 243 ACKYAYRNATPGTTLGDEYFLSRLPVVEKRLAQGGIRLAATLNRIFS 288

[Close]
BLAST of Cucsa.126340 vs. Swiss-Prot
Match: ENDO3_ARATH (Endonuclease 3 OS=Arabidopsis thaliana GN=ENDO3 PE=2 SV=1)

HSP 1 Score: 261.5 bits (667), Expect = 1.1e-68
Identity = 136/283 (48.06%), Postives = 175/283 (61.84%), Query Frame = 1 

Query: 13  ISFLLVLPCAQG---WSKEGHILTCEIAQELLIPEAAEAVQDLLPESAGGNLSAMCVWPD 72
           +S L++     G   W   GH   C+IAQ     +   AV+ LLPESA G L+A+C WPD
Sbjct: 10  VSILVLTQLVNGALCWGDAGHYAVCKIAQSYFEEDTVVAVKKLLPESANGELAAVCSWPD 69

Query: 73  QIRLQSKYRWASPLHYANTPD-SCSFVYKRDCHNDAGQPDMCVAGAIRNFTTQLTTYRTQ 132
           +I+   ++RW S LH+A+TPD  C++ Y RDC  D      CV GAI N+T QL +    
Sbjct: 70  EIKKLPQWRWTSALHFADTPDYKCNYEYSRDCPKD-----WCVTGAIFNYTNQLMSTSEN 129

Query: 133 GFDSPH-NLTEALLFLSHFVGDIHQPLHVGFESDAGGNTIEVRWFRRKSNLHHVWDRDII 192
                H NLTEAL+FLSH++GDIHQPLH GF  D GGN I+V W+ +++NLH VWD  II
Sbjct: 130 SQSIVHYNLTEALMFLSHYMGDIHQPLHEGFIGDLGGNKIKVHWYNQETNLHRVWDDMII 189

Query: 193 LEALGDYYDKDGGLLLDELNRNLTQGIWSNDVSEWERCS-TVNSCVNRWADESTGLACKW 252
             AL  YY+     ++ EL   L  G WSNDV  WE C     +C N +A ES  LACK+
Sbjct: 190 ESALETYYNSSLPRMIHELQAKLKNG-WSNDVPSWESCQLNQTACPNPYASESIDLACKY 249

Query: 253 AYEGVEAGITLSEEYYDSRLPIVMERLAQGGVRLAMLLNRVFA 290
           AY    AG TL + Y+ SRLP+V +RLAQGG+RLA  LNR+F+
Sbjct: 250 AYRNATAGTTLGDYYFVSRLPVVEKRLAQGGIRLAGTLNRIFS 286

[Close]
BLAST of Cucsa.126340 vs. TrEMBL
Match: C3VEY2_CUCSA (Bifunctional nuclease OS=Cucumis sativus PE=2 SV=2)

HSP 1 Score: 624.8 bits (1610), Expect = 5.5e-176
Identity = 300/300 (100.00%), Postives = 300/300 (100.00%), Query Frame = 1 

Query: 1   MGKLVFLVVLIFISFLLVLPCAQGWSKEGHILTCEIAQELLIPEAAEAVQDLLPESAGGN 60
           MGKLVFLVVLIFISFLLVLPCAQGWSKEGHILTCEIAQELLIPEAAEAVQDLLPESAGGN
Sbjct: 1   MGKLVFLVVLIFISFLLVLPCAQGWSKEGHILTCEIAQELLIPEAAEAVQDLLPESAGGN 60

Query: 61  LSAMCVWPDQIRLQSKYRWASPLHYANTPDSCSFVYKRDCHNDAGQPDMCVAGAIRNFTT 120
           LSAMCVWPDQIRLQSKYRWASPLHYANTPDSCSFVYKRDCHNDAGQPDMCVAGAIRNFTT
Sbjct: 61  LSAMCVWPDQIRLQSKYRWASPLHYANTPDSCSFVYKRDCHNDAGQPDMCVAGAIRNFTT 120

Query: 121 QLTTYRTQGFDSPHNLTEALLFLSHFVGDIHQPLHVGFESDAGGNTIEVRWFRRKSNLHH 180
           QLTTYRTQGFDSPHNLTEALLFLSHFVGDIHQPLHVGFESDAGGNTIEVRWFRRKSNLHH
Sbjct: 121 QLTTYRTQGFDSPHNLTEALLFLSHFVGDIHQPLHVGFESDAGGNTIEVRWFRRKSNLHH 180

Query: 181 VWDRDIILEALGDYYDKDGGLLLDELNRNLTQGIWSNDVSEWERCSTVNSCVNRWADEST 240
           VWDRDIILEALGDYYDKDGGLLLDELNRNLTQGIWSNDVSEWERCSTVNSCVNRWADEST
Sbjct: 181 VWDRDIILEALGDYYDKDGGLLLDELNRNLTQGIWSNDVSEWERCSTVNSCVNRWADEST 240

Query: 241 GLACKWAYEGVEAGITLSEEYYDSRLPIVMERLAQGGVRLAMLLNRVFAEDATRGFAYSS 300
           GLACKWAYEGVEAGITLSEEYYDSRLPIVMERLAQGGVRLAMLLNRVFAEDATRGFAYSS
Sbjct: 241 GLACKWAYEGVEAGITLSEEYYDSRLPIVMERLAQGGVRLAMLLNRVFAEDATRGFAYSS 300

[Close]
BLAST of Cucsa.126340 vs. TrEMBL
Match: A0A0A0LUG7_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_1G072450 PE=4 SV=1)

HSP 1 Score: 503.4 bits (1295), Expect = 1.9e-139
Identity = 237/237 (100.00%), Postives = 237/237 (100.00%), Query Frame = 1 

Query: 64  MCVWPDQIRLQSKYRWASPLHYANTPDSCSFVYKRDCHNDAGQPDMCVAGAIRNFTTQLT 123
           MCVWPDQIRLQSKYRWASPLHYANTPDSCSFVYKRDCHNDAGQPDMCVAGAIRNFTTQLT
Sbjct: 1   MCVWPDQIRLQSKYRWASPLHYANTPDSCSFVYKRDCHNDAGQPDMCVAGAIRNFTTQLT 60

Query: 124 TYRTQGFDSPHNLTEALLFLSHFVGDIHQPLHVGFESDAGGNTIEVRWFRRKSNLHHVWD 183
           TYRTQGFDSPHNLTEALLFLSHFVGDIHQPLHVGFESDAGGNTIEVRWFRRKSNLHHVWD
Sbjct: 61  TYRTQGFDSPHNLTEALLFLSHFVGDIHQPLHVGFESDAGGNTIEVRWFRRKSNLHHVWD 120

Query: 184 RDIILEALGDYYDKDGGLLLDELNRNLTQGIWSNDVSEWERCSTVNSCVNRWADESTGLA 243
           RDIILEALGDYYDKDGGLLLDELNRNLTQGIWSNDVSEWERCSTVNSCVNRWADESTGLA
Sbjct: 121 RDIILEALGDYYDKDGGLLLDELNRNLTQGIWSNDVSEWERCSTVNSCVNRWADESTGLA 180

Query: 244 CKWAYEGVEAGITLSEEYYDSRLPIVMERLAQGGVRLAMLLNRVFAEDATRGFAYSS 301
           CKWAYEGVEAGITLSEEYYDSRLPIVMERLAQGGVRLAMLLNRVFAEDATRGFAYSS
Sbjct: 181 CKWAYEGVEAGITLSEEYYDSRLPIVMERLAQGGVRLAMLLNRVFAEDATRGFAYSS 237

[Close]
BLAST of Cucsa.126340 vs. TrEMBL
Match: A0A151T7J6_CAJCA (Nuclease PA3 OS=Cajanus cajan GN=KK1_017575 PE=4 SV=1)

HSP 1 Score: 406.0 bits (1042), Expect = 4.0e-110
Identity = 188/298 (63.09%), Postives = 230/298 (77.18%), Query Frame = 1 

Query: 1   MGKLVFLVVLIFISFLLVLPCAQGWSKEGHILTCEIAQELLIPEAAEAVQDLLPESAGGN 60
           M  L F  +L F S  + +P A  WSKEGH++TC+IAQ LL PEAAEAV  LLPE   GN
Sbjct: 9   MSLLPFGFLLFFTSAFIGVPGALAWSKEGHVMTCQIAQALLEPEAAEAVHHLLPEYVNGN 68

Query: 61  LSAMCVWPDQIRLQSKYRWASPLHYANTPDS-CSFVYKRDCHNDAGQPDMCVAGAIRNFT 120
           LSA+C+WPDQIR   KYRW SPLH+ +TPD+ CSF Y RDCH+  G  DMCVAGA++NFT
Sbjct: 69  LSALCIWPDQIRHWYKYRWTSPLHFIDTPDNACSFKYSRDCHDPHGVEDMCVAGAVKNFT 128

Query: 121 TQLTTYRTQGFDSPHNLTEALLFLSHFVGDIHQPLHVGFESDAGGNTIEVRWFRRKSNLH 180
           +QL  Y     D  HN+TEALLFLSHF+GDIHQP+HVGF +D GGNTI +RWFR KSNLH
Sbjct: 129 SQLMHYSEGTSDRRHNMTEALLFLSHFMGDIHQPMHVGFTTDEGGNTINLRWFRHKSNLH 188

Query: 181 HVWDRDIILEALGDYYDKDGGLLLDELNRNLTQGIWSNDVSEWERCSTVNSCVNRWADES 240
           HVWDR+IIL AL DYYDKD  LLL ++ RN T GIWS+DV+ W+ C+ ++ CVN WA ES
Sbjct: 189 HVWDREIILTALADYYDKDVSLLLQDIERNYTDGIWSDDVTSWKNCNDISQCVNNWAKES 248

Query: 241 TGLACKWAYEGVEAGITLSEEYYDSRLPIVMERLAQGGVRLAMLLNRVFAEDATRGFA 298
             +ACKW YEGVE+G TL+++Y+DSR+P VM+R+AQGG+RLAM+LN+VFA D+  G A
Sbjct: 249 IQIACKWGYEGVESGTTLADDYFDSRMPYVMKRIAQGGIRLAMILNKVFA-DSEEGLA 305

[Close]
BLAST of Cucsa.126340 vs. TrEMBL
Match: A0A0L9VGL9_PHAAN (Uncharacterized protein OS=Phaseolus angularis GN=LR48_Vigan09g250800 PE=4 SV=1)

HSP 1 Score: 404.1 bits (1037), Expect = 1.5e-109
Identity = 186/296 (62.84%), Postives = 231/296 (78.04%), Query Frame = 1 

Query: 6   FLVVLIFISFLLVLPCAQGWSKEGHILTCEIAQELLIPEAAEAVQDLLPESAGGNLSAMC 65
           F  VL F S  + +P A GWSKEGH++TC+IAQ LL PEA+EAV  LLP+   GNLSA+C
Sbjct: 15  FGFVLFFSSTFIGVPSALGWSKEGHVMTCQIAQALLEPEASEAVNRLLPDYVKGNLSALC 74

Query: 66  VWPDQIRLQSKYRWASPLHYANTPDS-CSFVYKRDCHNDAGQPDMCVAGAIRNFTTQLTT 125
           VWPDQIR   KYRW SPLH+ +TPD+ CSF Y RDCH+  G  DMCVAGA++NFT+QL  
Sbjct: 75  VWPDQIRHGYKYRWTSPLHFIDTPDNACSFQYSRDCHDSHGVEDMCVAGAVKNFTSQLKQ 134

Query: 126 YRTQGFDSPHNLTEALLFLSHFVGDIHQPLHVGFESDAGGNTIEVRWFRRKSNLHHVWDR 185
           YR    D  +N+TEALLFLSHF+GDIHQP+HVGF +D GGNTIE+RWFR KSNLHHVWDR
Sbjct: 135 YREGTSDQRYNMTEALLFLSHFMGDIHQPMHVGFTTDEGGNTIELRWFRHKSNLHHVWDR 194

Query: 186 DIILEALGDYYDKDGGLLLDELNRNLTQGIWSNDVSEWERCSTVNSCVNRWADESTGLAC 245
           +IIL AL DYYDKD  LLL ++ RN T GIW +D+S W+ C+ ++ CVN WA ES  +AC
Sbjct: 195 EIILSALADYYDKDVSLLLQDIQRNYTYGIWVDDISSWKHCNDISQCVNDWAKESIQIAC 254

Query: 246 KWAYEGVEAGITLSEEYYDSRLPIVMERLAQGGVRLAMLLNRVFAEDATRGFAYSS 301
           KW YEGV++G TL++ Y+DSR+P VM+R+AQGG+RLAM+LN+VFA  +  GF Y++
Sbjct: 255 KWGYEGVKSGTTLADYYFDSRMPFVMKRIAQGGIRLAMVLNKVFAA-SEEGFTYNN 309

[Close]
BLAST of Cucsa.126340 vs. TrEMBL
Match: A0A0S3S2E7_PHAAN (Uncharacterized protein OS=Vigna angularis var. angularis GN=Vigan.05G034500 PE=4 SV=1)

HSP 1 Score: 404.1 bits (1037), Expect = 1.5e-109
Identity = 186/296 (62.84%), Postives = 231/296 (78.04%), Query Frame = 1 

Query: 6   FLVVLIFISFLLVLPCAQGWSKEGHILTCEIAQELLIPEAAEAVQDLLPESAGGNLSAMC 65
           F  VL F S  + +P A GWSKEGH++TC+IAQ LL PEA+EAV  LLP+   GNLSA+C
Sbjct: 15  FGFVLFFSSTFIGVPSALGWSKEGHVMTCQIAQALLEPEASEAVNRLLPDYVKGNLSALC 74

Query: 66  VWPDQIRLQSKYRWASPLHYANTPDS-CSFVYKRDCHNDAGQPDMCVAGAIRNFTTQLTT 125
           VWPDQIR   KYRW SPLH+ +TPD+ CSF Y RDCH+  G  DMCVAGA++NFT+QL  
Sbjct: 75  VWPDQIRHGYKYRWTSPLHFIDTPDNACSFQYSRDCHDSHGVEDMCVAGAVKNFTSQLKQ 134

Query: 126 YRTQGFDSPHNLTEALLFLSHFVGDIHQPLHVGFESDAGGNTIEVRWFRRKSNLHHVWDR 185
           YR    D  +N+TEALLFLSHF+GDIHQP+HVGF +D GGNTIE+RWFR KSNLHHVWDR
Sbjct: 135 YREGTSDQRYNMTEALLFLSHFMGDIHQPMHVGFTTDEGGNTIELRWFRHKSNLHHVWDR 194

Query: 186 DIILEALGDYYDKDGGLLLDELNRNLTQGIWSNDVSEWERCSTVNSCVNRWADESTGLAC 245
           +IIL AL DYYDKD  LLL ++ RN T GIW +D+S W+ C+ ++ CVN WA ES  +AC
Sbjct: 195 EIILSALADYYDKDVSLLLQDIQRNYTYGIWVDDISSWKHCNDISQCVNDWAKESIQIAC 254

Query: 246 KWAYEGVEAGITLSEEYYDSRLPIVMERLAQGGVRLAMLLNRVFAEDATRGFAYSS 301
           KW YEGV++G TL++ Y+DSR+P VM+R+AQGG+RLAM+LN+VFA  +  GF Y++
Sbjct: 255 KWGYEGVKSGTTLADYYFDSRMPFVMKRIAQGGIRLAMVLNKVFAA-SEEGFTYNN 309

[Close]
BLAST of Cucsa.126340 vs. TAIR10
Match: AT1G11190.1 (AT1G11190.1 bifunctional nuclease i)

HSP 1 Score: 359.8 bits (922), Expect = 1.7e-99
Identity = 167/286 (58.39%), Postives = 219/286 (76.57%), Query Frame = 1 

Query: 7   LVVLIFISFLLVLPCAQGWSKEGHILTCEIAQELLIPEAAEAVQDLLPESAGGNLSAMCV 66
           L++++ I  L  +   + WSKEGHILTC IAQ LL    A  V++LLP+   G+LSA+CV
Sbjct: 11  LILVLGILILCSVSSVRSWSKEGHILTCRIAQNLLEAGPAHVVENLLPDYVKGDLSALCV 70

Query: 67  WPDQIRLQSKYRWASPLHYANTPD-SCSFVYKRDCHNDAGQPDMCVAGAIRNFTTQLTTY 126
           WPDQIR   KYRW S LHY +TPD +CS+ Y RDCH+  G  DMCV GAI+NFT+QL  Y
Sbjct: 71  WPDQIRHWYKYRWTSHLHYIDTPDQACSYEYSRDCHDQHGLKDMCVDGAIQNFTSQLQHY 130

Query: 127 RTQGFDSPHNLTEALLFLSHFVGDIHQPLHVGFESDAGGNTIEVRWFRRKSNLHHVWDRD 186
                D  +N+TEALLFLSHF+GDIHQP+HVGF SD GGNTI++RW++ KSNLHHVWDR+
Sbjct: 131 GEGTSDRRYNMTEALLFLSHFMGDIHQPMHVGFTSDEGGNTIDLRWYKHKSNLHHVWDRE 190

Query: 187 IILEALGDYYDKDGGLLLDELNRNLTQGIWSNDVSEWERCSTVNSCVNRWADESTGLACK 246
           IIL AL + YDK+  LL ++L +N+T G+W +D+S W  C+ + +C +++A ES  LACK
Sbjct: 191 IILTALKENYDKNLDLLQEDLEKNITNGLWHDDLSSWTECNDLIACPHKYASESIKLACK 250

Query: 247 WAYEGVEAGITLSEEYYDSRLPIVMERLAQGGVRLAMLLNRVFAED 292
           W Y+GV++G TLSEEY+++RLPIVM+R+ QGGVRLAM+LNRVF++D
Sbjct: 251 WGYKGVKSGETLSEEYFNTRLPIVMKRIVQGGVRLAMILNRVFSDD 296

[Close]
BLAST of Cucsa.126340 vs. TAIR10
Match: AT1G68290.1 (AT1G68290.1 endonuclease 2)

HSP 1 Score: 313.5 bits (802), Expect = 1.4e-85
Identity = 149/283 (52.65%), Postives = 201/283 (71.02%), Query Frame = 1 

Query: 7   LVVLIFISFLLVLPCAQGWSKEGHILTCEIAQELLIPEAAEAVQDLLPESAGGNLSAMCV 66
           ++++I +  L   P   GW KEGH + C+IAQ  L   AA+AV++LLPESA G+LS++C+
Sbjct: 10  VMMIITVWLLYAAPNIHGWGKEGHEIICKIAQTRLDETAAKAVKELLPESAEGDLSSLCL 69

Query: 67  WPDQIRLQSKYRWASPLHYANTPDSCSFVYKRDCHNDAGQPDMCVAGAIRNFTTQLTTYR 126
           W D+++   +Y W+SPLHY NTPD+CS+ Y RDC +++G+   CVAGAI N+TTQL +Y+
Sbjct: 70  WADRVKF--RYHWSSPLHYINTPDACSYQYNRDCKDESGEKGRCVAGAIYNYTTQLLSYK 129

Query: 127 TQGFD-SPHNLTEALLFLSHFVGDIHQPLHVGFESDAGGNTIEVRWFRRKSNLHHVWDRD 186
           T     S +NLTEALLF+SHF+GDIHQPLHV + SD GGNTIEV W+ RK+NLHH+WD +
Sbjct: 130 TAASSQSQYNLTEALLFVSHFMGDIHQPLHVSYASDKGGNTIEVHWYTRKANLHHIWDSN 189

Query: 187 IILEALGDYYDKDGGLLLDELNRNLTQGIWSNDVSEWERCSTVNSCVNRWADESTGLACK 246
           II  A  D Y+     ++D L +N+T   W++ V  WE C+   +C + +A E    AC 
Sbjct: 190 IIETAEADLYNSALEGMVDALKKNITTE-WADQVKRWETCTKKTACPDIYASEGIQAACD 249

Query: 247 WAYEGVEAGITLSEEYYDSRLPIVMERLAQGGVRLAMLLNRVF 289
           WAY+GV  G TL +EY+ SRLPIV +RLAQGGVRLA  LNR+F
Sbjct: 250 WAYKGVTEGDTLEDEYFYSRLPIVYQRLAQGGVRLAATLNRIF 289

[Close]
BLAST of Cucsa.126340 vs. TAIR10
Match: AT4G21585.1 (AT4G21585.1 endonuclease 4)

HSP 1 Score: 292.7 bits (748), Expect = 2.5e-79
Identity = 145/268 (54.10%), Postives = 181/268 (67.54%), Query Frame = 1 

Query: 25  WSKEGHILTCEIAQELLIPEAAEAVQDLLPESAGGNLSAMCVWPDQIRLQSKYRWASPLH 84
           W KEGH   C+IA+     E   AV+ LLP+SA G+L+++C WPD+I+   ++RW SPLH
Sbjct: 25  WGKEGHYTVCKIAESYFEEETVAAVKKLLPKSADGDLASVCSWPDEIKHHWQWRWTSPLH 84

Query: 85  YANTPD-SCSFVYKRDCHNDAGQPDMCVAGAIRNFTTQLTTYRTQGFDSPH-NLTEALLF 144
           Y +TPD  C++ Y RDCH+     D CV GAI N+T QL +         H NLTEAL+F
Sbjct: 85  YVDTPDYRCNYEYCRDCHDTHKNQDRCVTGAIFNYTMQLMSASENSDTIVHYNLTEALMF 144

Query: 145 LSHFVGDIHQPLHVGFESDAGGNTIEVRWFRRKSNLHHVWDRDIILEALGDYYDKDGGLL 204
           LSHF+GDIHQPLHVGF  D GGNTI VRW+RRK+NLHHVWD  II  AL  YY+K   L+
Sbjct: 145 LSHFIGDIHQPLHVGFLGDEGGNTITVRWYRRKTNLHHVWDNMIIESALKTYYNKSLPLM 204

Query: 205 LDELNRNLTQGIWSNDVSEWERCS-TVNSCVNRWADESTGLACKWAYEGVEAGITLSEEY 264
           ++ L  NLT   WSNDV  WE C     +C N +A ES  LACK+AY     G TL ++Y
Sbjct: 205 IEALQANLTND-WSNDVPLWESCQLNQTACPNPYASESINLACKYAYRNATPGTTLGDDY 264

Query: 265 YDSRLPIVMERLAQGGVRLAMLLNRVFA 290
           + SRLPIV +RLAQGG+RLA  LNR+F+
Sbjct: 265 FLSRLPIVEKRLAQGGIRLAATLNRIFS 291

[Close]
BLAST of Cucsa.126340 vs. TAIR10
Match: AT4G21600.1 (AT4G21600.1 endonuclease 5)

HSP 1 Score: 267.3 bits (682), Expect = 1.1e-71
Identity = 138/287 (48.08%), Postives = 178/287 (62.02%), Query Frame = 1 

Query: 10  LIFISFLLVLPCAQG---WSKEGHILTCEIAQELLIPEAAEAVQDLLPESA-GGNLSAMC 69
           L  +S L++     G   W K+GH   C++A+     +   AV+ LLPES  GG L+  C
Sbjct: 3   LWIVSVLVLTHLVHGALCWGKDGHYTVCKLAEGFFEDDTIAAVKKLLPESVDGGGLADFC 62

Query: 70  VWPDQIRLQSKYRWASPLHYANTPD-SCSFVYKRDCHNDAGQPDMCVAGAIRNFTTQLTT 129
            WPD+I+  S+++W S LHY NTP+  C++ Y RDCH+     D CV GAI N+T QL +
Sbjct: 63  SWPDEIKKLSQWQWTSTLHYVNTPEYRCNYEYCRDCHDTHKHKDWCVTGAIFNYTNQLMS 122

Query: 130 YRTQGFDSPH-NLTEALLFLSHFVGDIHQPLHVGFESDAGGNTIEVRWFRRKSNLHHVWD 189
                 +  H NLTEALLFLSH++GD+HQPLH GF  D GGNTI V W+  KSNLHHVWD
Sbjct: 123 ASENSQNIVHYNLTEALLFLSHYMGDVHQPLHTGFLGDLGGNTIIVNWYHNKSNLHHVWD 182

Query: 190 RDIILEALGDYYDKDGGLLLDELNRNLTQGIWSNDVSEWERCS-TVNSCVNRWADESTGL 249
             II  AL  YY+     ++  L   L  G WSNDV  W+ C     +C N +A ES  L
Sbjct: 183 NMIIDSALETYYNSSLPHMIQALQAKLKNG-WSNDVPSWKSCHFHQKACPNLYASESIDL 242

Query: 250 ACKWAYEGVEAGITLSEEYYDSRLPIVMERLAQGGVRLAMLLNRVFA 290
           ACK+AY     G TL +EY+ SRLP+V +RLAQGG+RLA  LNR+F+
Sbjct: 243 ACKYAYRNATPGTTLGDEYFLSRLPVVEKRLAQGGIRLAATLNRIFS 288

[Close]
BLAST of Cucsa.126340 vs. TAIR10
Match: AT4G21590.1 (AT4G21590.1 endonuclease 3)

HSP 1 Score: 261.5 bits (667), Expect = 6.2e-70
Identity = 136/283 (48.06%), Postives = 175/283 (61.84%), Query Frame = 1 

Query: 13  ISFLLVLPCAQG---WSKEGHILTCEIAQELLIPEAAEAVQDLLPESAGGNLSAMCVWPD 72
           +S L++     G   W   GH   C+IAQ     +   AV+ LLPESA G L+A+C WPD
Sbjct: 10  VSILVLTQLVNGALCWGDAGHYAVCKIAQSYFEEDTVVAVKKLLPESANGELAAVCSWPD 69

Query: 73  QIRLQSKYRWASPLHYANTPD-SCSFVYKRDCHNDAGQPDMCVAGAIRNFTTQLTTYRTQ 132
           +I+   ++RW S LH+A+TPD  C++ Y RDC  D      CV GAI N+T QL +    
Sbjct: 70  EIKKLPQWRWTSALHFADTPDYKCNYEYSRDCPKD-----WCVTGAIFNYTNQLMSTSEN 129

Query: 133 GFDSPH-NLTEALLFLSHFVGDIHQPLHVGFESDAGGNTIEVRWFRRKSNLHHVWDRDII 192
                H NLTEAL+FLSH++GDIHQPLH GF  D GGN I+V W+ +++NLH VWD  II
Sbjct: 130 SQSIVHYNLTEALMFLSHYMGDIHQPLHEGFIGDLGGNKIKVHWYNQETNLHRVWDDMII 189

Query: 193 LEALGDYYDKDGGLLLDELNRNLTQGIWSNDVSEWERCS-TVNSCVNRWADESTGLACKW 252
             AL  YY+     ++ EL   L  G WSNDV  WE C     +C N +A ES  LACK+
Sbjct: 190 ESALETYYNSSLPRMIHELQAKLKNG-WSNDVPSWESCQLNQTACPNPYASESIDLACKY 249

Query: 253 AYEGVEAGITLSEEYYDSRLPIVMERLAQGGVRLAMLLNRVFA 290
           AY    AG TL + Y+ SRLP+V +RLAQGG+RLA  LNR+F+
Sbjct: 250 AYRNATAGTTLGDYYFVSRLPVVEKRLAQGGIRLAGTLNRIFS 286

[Close]
BLAST of Cucsa.126340 vs. NCBI nr
Match: gi|793421441|ref|NP_001292654.1| (endonuclease 1 precursor [Cucumis sativus])

HSP 1 Score: 624.8 bits (1610), Expect = 7.9e-176
Identity = 300/300 (100.00%), Postives = 300/300 (100.00%), Query Frame = 1 

Query: 1   MGKLVFLVVLIFISFLLVLPCAQGWSKEGHILTCEIAQELLIPEAAEAVQDLLPESAGGN 60
           MGKLVFLVVLIFISFLLVLPCAQGWSKEGHILTCEIAQELLIPEAAEAVQDLLPESAGGN
Sbjct: 1   MGKLVFLVVLIFISFLLVLPCAQGWSKEGHILTCEIAQELLIPEAAEAVQDLLPESAGGN 60

Query: 61  LSAMCVWPDQIRLQSKYRWASPLHYANTPDSCSFVYKRDCHNDAGQPDMCVAGAIRNFTT 120
           LSAMCVWPDQIRLQSKYRWASPLHYANTPDSCSFVYKRDCHNDAGQPDMCVAGAIRNFTT
Sbjct: 61  LSAMCVWPDQIRLQSKYRWASPLHYANTPDSCSFVYKRDCHNDAGQPDMCVAGAIRNFTT 120

Query: 121 QLTTYRTQGFDSPHNLTEALLFLSHFVGDIHQPLHVGFESDAGGNTIEVRWFRRKSNLHH 180
           QLTTYRTQGFDSPHNLTEALLFLSHFVGDIHQPLHVGFESDAGGNTIEVRWFRRKSNLHH
Sbjct: 121 QLTTYRTQGFDSPHNLTEALLFLSHFVGDIHQPLHVGFESDAGGNTIEVRWFRRKSNLHH 180

Query: 181 VWDRDIILEALGDYYDKDGGLLLDELNRNLTQGIWSNDVSEWERCSTVNSCVNRWADEST 240
           VWDRDIILEALGDYYDKDGGLLLDELNRNLTQGIWSNDVSEWERCSTVNSCVNRWADEST
Sbjct: 181 VWDRDIILEALGDYYDKDGGLLLDELNRNLTQGIWSNDVSEWERCSTVNSCVNRWADEST 240

Query: 241 GLACKWAYEGVEAGITLSEEYYDSRLPIVMERLAQGGVRLAMLLNRVFAEDATRGFAYSS 300
           GLACKWAYEGVEAGITLSEEYYDSRLPIVMERLAQGGVRLAMLLNRVFAEDATRGFAYSS
Sbjct: 241 GLACKWAYEGVEAGITLSEEYYDSRLPIVMERLAQGGVRLAMLLNRVFAEDATRGFAYSS 300

[Close]
BLAST of Cucsa.126340 vs. NCBI nr
Match: gi|659067951|ref|XP_008442043.1| (PREDICTED: endonuclease 1 [Cucumis melo])

HSP 1 Score: 573.5 bits (1477), Expect = 2.1e-160
Identity = 277/302 (91.72%), Postives = 285/302 (94.37%), Query Frame = 1 

Query: 1   MGKLVF--LVVLIFISFLLVLPCAQGWSKEGHILTCEIAQELLIPEAAEAVQDLLPESAG 60
           MGKL F  LVVL FISFLLVLPCAQGWSKEGHILTCEIAQELL PEAA+AVQDLLPESAG
Sbjct: 1   MGKLAFRFLVVLSFISFLLVLPCAQGWSKEGHILTCEIAQELLNPEAADAVQDLLPESAG 60

Query: 61  GNLSAMCVWPDQIRLQSKYRWASPLHYANTPDSCSFVYKRDCHNDAGQPDMCVAGAIRNF 120
           GNLSAMCVW DQIRLQSKYRWASPLHYANTPDSCSFVYKRDCHNDAGQPDMCVAGAIRNF
Sbjct: 61  GNLSAMCVWADQIRLQSKYRWASPLHYANTPDSCSFVYKRDCHNDAGQPDMCVAGAIRNF 120

Query: 121 TTQLTTYRTQGFDSPHNLTEALLFLSHFVGDIHQPLHVGFESDAGGNTIEVRWFRRKSNL 180
           TTQLTTYRTQG DSPHNLTEALLFLSHFVGDIHQPLHVGF SD GGNTIEVRWFRRKSNL
Sbjct: 121 TTQLTTYRTQGSDSPHNLTEALLFLSHFVGDIHQPLHVGFASDEGGNTIEVRWFRRKSNL 180

Query: 181 HHVWDRDIILEALGDYYDKDGGLLLDELNRNLTQGIWSNDVSEWERCSTVNSCVNRWADE 240
           HHVWDRDIIL AL DYYDKDGGLLL+EL RNLTQGIWSNDV  WERC  VNSCVN+WA+E
Sbjct: 181 HHVWDRDIILTALADYYDKDGGLLLEELQRNLTQGIWSNDVPTWERCVKVNSCVNKWAEE 240

Query: 241 STGLACKWAYEGVEAGITLSEEYYDSRLPIVMERLAQGGVRLAMLLNRVFAEDATRGFAY 300
           ST LACKWAYEGVEAGITLSE+Y+DSRLPIVMERLAQGGVRLAMLLNRVF+EDAT+GFAY
Sbjct: 241 STDLACKWAYEGVEAGITLSEDYFDSRLPIVMERLAQGGVRLAMLLNRVFSEDATQGFAY 300

[Close]
BLAST of Cucsa.126340 vs. NCBI nr
Match: gi|700209538|gb|KGN64634.1| (hypothetical protein Csa_1G072450 [Cucumis sativus])

HSP 1 Score: 503.4 bits (1295), Expect = 2.7e-139
Identity = 237/237 (100.00%), Postives = 237/237 (100.00%), Query Frame = 1 

Query: 64  MCVWPDQIRLQSKYRWASPLHYANTPDSCSFVYKRDCHNDAGQPDMCVAGAIRNFTTQLT 123
           MCVWPDQIRLQSKYRWASPLHYANTPDSCSFVYKRDCHNDAGQPDMCVAGAIRNFTTQLT
Sbjct: 1   MCVWPDQIRLQSKYRWASPLHYANTPDSCSFVYKRDCHNDAGQPDMCVAGAIRNFTTQLT 60

Query: 124 TYRTQGFDSPHNLTEALLFLSHFVGDIHQPLHVGFESDAGGNTIEVRWFRRKSNLHHVWD 183
           TYRTQGFDSPHNLTEALLFLSHFVGDIHQPLHVGFESDAGGNTIEVRWFRRKSNLHHVWD
Sbjct: 61  TYRTQGFDSPHNLTEALLFLSHFVGDIHQPLHVGFESDAGGNTIEVRWFRRKSNLHHVWD 120

Query: 184 RDIILEALGDYYDKDGGLLLDELNRNLTQGIWSNDVSEWERCSTVNSCVNRWADESTGLA 243
           RDIILEALGDYYDKDGGLLLDELNRNLTQGIWSNDVSEWERCSTVNSCVNRWADESTGLA
Sbjct: 121 RDIILEALGDYYDKDGGLLLDELNRNLTQGIWSNDVSEWERCSTVNSCVNRWADESTGLA 180

Query: 244 CKWAYEGVEAGITLSEEYYDSRLPIVMERLAQGGVRLAMLLNRVFAEDATRGFAYSS 301
           CKWAYEGVEAGITLSEEYYDSRLPIVMERLAQGGVRLAMLLNRVFAEDATRGFAYSS
Sbjct: 181 CKWAYEGVEAGITLSEEYYDSRLPIVMERLAQGGVRLAMLLNRVFAEDATRGFAYSS 237

[Close]
BLAST of Cucsa.126340 vs. NCBI nr
Match: gi|1012351824|gb|KYP63013.1| (Nuclease PA3 [Cajanus cajan])

HSP 1 Score: 406.0 bits (1042), Expect = 5.8e-110
Identity = 188/298 (63.09%), Postives = 230/298 (77.18%), Query Frame = 1 

Query: 1   MGKLVFLVVLIFISFLLVLPCAQGWSKEGHILTCEIAQELLIPEAAEAVQDLLPESAGGN 60
           M  L F  +L F S  + +P A  WSKEGH++TC+IAQ LL PEAAEAV  LLPE   GN
Sbjct: 9   MSLLPFGFLLFFTSAFIGVPGALAWSKEGHVMTCQIAQALLEPEAAEAVHHLLPEYVNGN 68

Query: 61  LSAMCVWPDQIRLQSKYRWASPLHYANTPDS-CSFVYKRDCHNDAGQPDMCVAGAIRNFT 120
           LSA+C+WPDQIR   KYRW SPLH+ +TPD+ CSF Y RDCH+  G  DMCVAGA++NFT
Sbjct: 69  LSALCIWPDQIRHWYKYRWTSPLHFIDTPDNACSFKYSRDCHDPHGVEDMCVAGAVKNFT 128

Query: 121 TQLTTYRTQGFDSPHNLTEALLFLSHFVGDIHQPLHVGFESDAGGNTIEVRWFRRKSNLH 180
           +QL  Y     D  HN+TEALLFLSHF+GDIHQP+HVGF +D GGNTI +RWFR KSNLH
Sbjct: 129 SQLMHYSEGTSDRRHNMTEALLFLSHFMGDIHQPMHVGFTTDEGGNTINLRWFRHKSNLH 188

Query: 181 HVWDRDIILEALGDYYDKDGGLLLDELNRNLTQGIWSNDVSEWERCSTVNSCVNRWADES 240
           HVWDR+IIL AL DYYDKD  LLL ++ RN T GIWS+DV+ W+ C+ ++ CVN WA ES
Sbjct: 189 HVWDREIILTALADYYDKDVSLLLQDIERNYTDGIWSDDVTSWKNCNDISQCVNNWAKES 248

Query: 241 TGLACKWAYEGVEAGITLSEEYYDSRLPIVMERLAQGGVRLAMLLNRVFAEDATRGFA 298
             +ACKW YEGVE+G TL+++Y+DSR+P VM+R+AQGG+RLAM+LN+VFA D+  G A
Sbjct: 249 IQIACKWGYEGVESGTTLADDYFDSRMPYVMKRIAQGGIRLAMILNKVFA-DSEEGLA 305

[Close]
BLAST of Cucsa.126340 vs. NCBI nr
Match: gi|920712600|gb|KOM53849.1| (hypothetical protein LR48_Vigan09g250800 [Vigna angularis])

HSP 1 Score: 404.1 bits (1037), Expect = 2.2e-109
Identity = 186/296 (62.84%), Postives = 231/296 (78.04%), Query Frame = 1 

Query: 6   FLVVLIFISFLLVLPCAQGWSKEGHILTCEIAQELLIPEAAEAVQDLLPESAGGNLSAMC 65
           F  VL F S  + +P A GWSKEGH++TC+IAQ LL PEA+EAV  LLP+   GNLSA+C
Sbjct: 15  FGFVLFFSSTFIGVPSALGWSKEGHVMTCQIAQALLEPEASEAVNRLLPDYVKGNLSALC 74

Query: 66  VWPDQIRLQSKYRWASPLHYANTPDS-CSFVYKRDCHNDAGQPDMCVAGAIRNFTTQLTT 125
           VWPDQIR   KYRW SPLH+ +TPD+ CSF Y RDCH+  G  DMCVAGA++NFT+QL  
Sbjct: 75  VWPDQIRHGYKYRWTSPLHFIDTPDNACSFQYSRDCHDSHGVEDMCVAGAVKNFTSQLKQ 134

Query: 126 YRTQGFDSPHNLTEALLFLSHFVGDIHQPLHVGFESDAGGNTIEVRWFRRKSNLHHVWDR 185
           YR    D  +N+TEALLFLSHF+GDIHQP+HVGF +D GGNTIE+RWFR KSNLHHVWDR
Sbjct: 135 YREGTSDQRYNMTEALLFLSHFMGDIHQPMHVGFTTDEGGNTIELRWFRHKSNLHHVWDR 194

Query: 186 DIILEALGDYYDKDGGLLLDELNRNLTQGIWSNDVSEWERCSTVNSCVNRWADESTGLAC 245
           +IIL AL DYYDKD  LLL ++ RN T GIW +D+S W+ C+ ++ CVN WA ES  +AC
Sbjct: 195 EIILSALADYYDKDVSLLLQDIQRNYTYGIWVDDISSWKHCNDISQCVNDWAKESIQIAC 254

Query: 246 KWAYEGVEAGITLSEEYYDSRLPIVMERLAQGGVRLAMLLNRVFAEDATRGFAYSS 301
           KW YEGV++G TL++ Y+DSR+P VM+R+AQGG+RLAM+LN+VFA  +  GF Y++
Sbjct: 255 KWGYEGVKSGTTLADYYFDSRMPFVMKRIAQGGIRLAMVLNKVFAA-SEEGFTYNN 309

The following BLAST results are available for this feature:
BLAST of Cucsa.126340 vs. Swiss-Prot
Match NameE-valueIdentityDescription
ENDO1_ARATH3.0e-9858.39Endonuclease 1 OS=Arabidopsis thaliana GN=ENDO1 PE=1 SV=1[more]
ENDO2_ARATH2.4e-8452.65Endonuclease 2 OS=Arabidopsis thaliana GN=ENDO2 PE=1 SV=1[more]
ENDO4_ARATH4.5e-7854.10Endonuclease 4 OS=Arabidopsis thaliana GN=ENDO4 PE=3 SV=1[more]
ENDO5_ARATH2.0e-7048.08Endonuclease 5 OS=Arabidopsis thaliana GN=ENDO5 PE=2 SV=1[more]
ENDO3_ARATH1.1e-6848.06Endonuclease 3 OS=Arabidopsis thaliana GN=ENDO3 PE=2 SV=1[more]
BLAST of Cucsa.126340 vs. TrEMBL
Match NameE-valueIdentityDescription
C3VEY2_CUCSA5.5e-176100.00Bifunctional nuclease OS=Cucumis sativus PE=2 SV=2[more]
A0A0A0LUG7_CUCSA1.9e-139100.00Uncharacterized protein OS=Cucumis sativus GN=Csa_1G072450 PE=4 SV=1[more]
A0A151T7J6_CAJCA4.0e-11063.09Nuclease PA3 OS=Cajanus cajan GN=KK1_017575 PE=4 SV=1[more]
A0A0L9VGL9_PHAAN1.5e-10962.84Uncharacterized protein OS=Phaseolus angularis GN=LR48_Vigan09g250800 PE=4 SV=1[more]
A0A0S3S2E7_PHAAN1.5e-10962.84Uncharacterized protein OS=Vigna angularis var. angularis GN=Vigan.05G034500 PE=... [more]
BLAST of Cucsa.126340 vs. TAIR10
Match NameE-valueIdentityDescription
AT1G11190.11.7e-9958.39 bifunctional nuclease i[more]
AT1G68290.11.4e-8552.65 endonuclease 2[more]
AT4G21585.12.5e-7954.10 endonuclease 4[more]
AT4G21600.11.1e-7148.08 endonuclease 5[more]
AT4G21590.16.2e-7048.06 endonuclease 3[more]
BLAST of Cucsa.126340 vs. NCBI nr
Match NameE-valueIdentityDescription
gi|793421441|ref|NP_001292654.1|7.9e-176100.00endonuclease 1 precursor [Cucumis sativus][more]
gi|659067951|ref|XP_008442043.1|2.1e-16091.72PREDICTED: endonuclease 1 [Cucumis melo][more]
gi|700209538|gb|KGN64634.1|2.7e-139100.00hypothetical protein Csa_1G072450 [Cucumis sativus][more]
gi|1012351824|gb|KYP63013.1|5.8e-11063.09Nuclease PA3 [Cajanus cajan][more]
gi|920712600|gb|KOM53849.1|2.2e-10962.84hypothetical protein LR48_Vigan09g250800 [Vigna angularis][more]
Annotated Terms
The following terms have been associated with this gene:
Vocabulary: INTERPRO
TermDefinition
IPR003154S1/P1nuclease
IPR008947PLipase_C/P1_nuclease_dom_sf
Vocabulary: Molecular Function
TermDefinition
GO:0003676nucleic acid binding
GO:0004519endonuclease activity
GO:0016788hydrolase activity, acting on ester bonds
Vocabulary: Biological Process
TermDefinition
GO:0006308DNA catabolic process
GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006308 DNA catabolic process
biological_process GO:0090305 nucleic acid phosphodiester bond hydrolysis
cellular_component GO:0005575 cellular_component
molecular_function GO:0004519 endonuclease activity
molecular_function GO:0046872 metal ion binding
molecular_function GO:0003676 nucleic acid binding
molecular_function GO:0016788 hydrolase activity, acting on ester bonds
Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Cucsa.126340.1Cucsa.126340.1mRNA


InterPro
Analysis Name: InterPro Annotations of cucumber (Gy14)
Date Performed: 2017-01-17
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR003154S1/P1 nucleasePFAMPF02265S1-P1_nucleasecoord: 25..288
score: 7.9
IPR008947Phospholipase C/P1 nuclease domainGENE3DG3DSA:1.10.575.10coord: 25..290
score: 1.2
IPR008947Phospholipase C/P1 nuclease domainunknownSSF48537Phospholipase C/P1 nucleasecoord: 25..286
score: 3.79
NoneNo IPR availablePANTHERPTHR33146FAMILY NOT NAMEDcoord: 3..291
score: 3.6E
NoneNo IPR availablePANTHERPTHR33146:SF4ENDONUCLEASE 1coord: 3..291
score: 3.6E

Synteny
The following gene(s) are orthologous to this gene:
GeneOrthologueOrganismBlock
Cucsa.126340Cla019463Watermelon (97103) v1cgywmB205
Cucsa.126340Cla001356Watermelon (97103) v1cgywmB208
Cucsa.126340Csa1G072450Cucumber (Chinese Long) v2cgycuB170
Cucsa.126340MELO3C002562Melon (DHL92) v3.5.1cgymeB198
Cucsa.126340ClCG01G022610Watermelon (Charleston Gray)cgywcgB202
Cucsa.126340ClCG03G005590Watermelon (Charleston Gray)cgywcgB203
Cucsa.126340CSPI01G11700Wild cucumber (PI 183967)cgycpiB172
Cucsa.126340CmaCh13G006720Cucurbita maxima (Rimu)cgycmaB0336
Cucsa.126340CmaCh03G004840Cucurbita maxima (Rimu)cgycmaB0340
Cucsa.126340CmoCh13G006920Cucurbita moschata (Rifu)cgycmoB0330
Cucsa.126340Lsi01G003480Bottle gourd (USVL1VR-Ls)cgylsiB193
Cucsa.126340Lsi02G007090Bottle gourd (USVL1VR-Ls)cgylsiB194
Cucsa.126340Cp4.1LG20g04040Cucurbita pepo (Zucchini)cgycpeB0328
Cucsa.126340MELO3C002562.2Melon (DHL92) v3.6.1cgymedB197
Cucsa.126340CsaV3_1G011850Cucumber (Chinese Long) v3cgycucB174
Cucsa.126340Cla97C03G056900Watermelon (97103) v2cgywmbB200
Cucsa.126340Bhi08G001562Wax gourdcgywgoB232
Cucsa.126340Bhi03G000364Wax gourdcgywgoB230
Cucsa.126340Carg21478Silver-seed gourdcarcgyB1068
The following gene(s) are paralogous to this gene:

None

The following block(s) are covering this gene:
GeneOrganismBlock
Cucsa.126340Silver-seed gourdcarcgyB0182
Cucsa.126340Silver-seed gourdcarcgyB0716
Cucsa.126340Silver-seed gourdcarcgyB0809
Cucsa.126340Silver-seed gourdcarcgyB1022
Cucsa.126340Cucumber (Chinese Long) v3cgycucB175
Cucsa.126340Cucumber (Chinese Long) v3cgycucB176
Cucsa.126340Cucumber (Chinese Long) v3cgycucB177
Cucsa.126340Watermelon (97103) v2cgywmbB197
Cucsa.126340Watermelon (97103) v2cgywmbB198
Cucsa.126340Watermelon (97103) v2cgywmbB199
Cucsa.126340Cucumber (Gy14) v1cgycgyB004
Cucsa.126340Cucumber (Gy14) v1cgycgyB023
Cucsa.126340Cucumber (Gy14) v1cgycgyB076
Cucsa.126340Cucurbita maxima (Rimu)cgycmaB0335
Cucsa.126340Cucurbita maxima (Rimu)cgycmaB0334
Cucsa.126340Cucurbita maxima (Rimu)cgycmaB0337
Cucsa.126340Cucurbita maxima (Rimu)cgycmaB0338
Cucsa.126340Cucurbita maxima (Rimu)cgycmaB0341
Cucsa.126340Cucurbita moschata (Rifu)cgycmoB0329
Cucsa.126340Cucurbita moschata (Rifu)cgycmoB0331
Cucsa.126340Cucurbita moschata (Rifu)cgycmoB0332
Cucsa.126340Cucurbita moschata (Rifu)cgycmoB0333
Cucsa.126340Wild cucumber (PI 183967)cgycpiB173
Cucsa.126340Wild cucumber (PI 183967)cgycpiB174
Cucsa.126340Wild cucumber (PI 183967)cgycpiB175
Cucsa.126340Wild cucumber (PI 183967)cgycpiB176
Cucsa.126340Cucumber (Chinese Long) v2cgycuB171
Cucsa.126340Cucumber (Chinese Long) v2cgycuB172
Cucsa.126340Cucumber (Chinese Long) v2cgycuB173
Cucsa.126340Melon (DHL92) v3.5.1cgymeB199
Cucsa.126340Melon (DHL92) v3.5.1cgymeB200
Cucsa.126340Melon (DHL92) v3.5.1cgymeB201
Cucsa.126340Watermelon (Charleston Gray)cgywcgB201
Cucsa.126340Watermelon (97103) v1cgywmB209
Cucsa.126340Cucurbita pepo (Zucchini)cgycpeB0324
Cucsa.126340Cucurbita pepo (Zucchini)cgycpeB0326
Cucsa.126340Cucurbita pepo (Zucchini)cgycpeB0329
Cucsa.126340Cucurbita pepo (Zucchini)cgycpeB0330
Cucsa.126340Bottle gourd (USVL1VR-Ls)cgylsiB196
Cucsa.126340Melon (DHL92) v3.6.1cgymedB198
Cucsa.126340Melon (DHL92) v3.6.1cgymedB199