Cucsa.124520 (gene) Cucumber (Gy14) v1

NameCucsa.124520
Typegene
OrganismCucumis sativus (Cucumber (Gy14) v1)
DescriptionIsocitrate dehydrogenase [NAD] subunit, mitochondrial
Locationscaffold01001 : 425106 .. 429159 (+)
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
TCTTTGATTATTTTTCCTTCAATTTTtCGTCGTCAATCATGGCTTCGCAGCTCCTCAAACGCGCCCTTAGAAGCCAGTCTGGTCAGATCTTCTCCCTTTCATCTAGCCTTAACTCTTCCTCTCCCTTTCCTTTTAGGGCTTTTTCTTCCGCAGCTACTCCGATCCGAGCCACTCTCTTCCCCGGCGATGGTATTGGTCCCGAAATTGCGGAGTCCGTCAAACAGGTATTGCTTTCTTTCCATGATCTCAATTCTGTTTAACTACGATAATTGTCCCGTTTTTGTGTACCTTTTGAGAATTTCCCTTGCAATAACTCGAGACAATTGATCTGAATTTGTTACTTCAACGTTTAATCGTGTGGTGTTGGGGAGTATTTATTTTGTGTTTTATTTTATGAGAATTTGTTTATTTTGTTCATGTCGTTGAGTTTTTTTGTTTCTATTATTAGATGATGATTCAAGAGTACTGCCGTTTTTTTATCTTTCCAAATCGTAGTAGTGTCTCAATTACTCTTGAAAATAAGAGGAAAATTACTTGCGCTTACCATTTTACGGAGCATTAAAAACAAAGACCGCTAGATGATATAATAGTAAATTTTCCTTCATCCATTAGCTAGGGTCAATCAGTGATTTATGACTGTGTTTGATGATATTATACTAGATTTACTTTAGTCCTTTGGCTTAAGCTATTGGGTGGATGGTATCAGGGCAGGAGGTCCTTTATTTTGGTACGTTATTTTTTtCGTAATTAATATTGATTTTCACTTGTTGGATCTTCTACATATTCCAAGTCCAGGTGTGTGTGAAAATGTTTGACTATCTAGTAGTAAAATTATCTTCATCCATTAGCTTAAATTTTTTGGTCAATGGATAATTTAAGAGTTTTTTTTtCTCTTTTTATCGCCAAATGATATTCACGGCGTTGTGTGATGTATTTCATTTTTCACATGTACAGGTGTTTAGAACTGCTGATGTGCCCATCGAATGGGAAGAACACTACGTTGGGGACGAAATAGATCCTCGAACTCAAAGTTTCCTCACATGGGAAAGCTTAGAATCAGTGAGACGTAATGGTGTGGGTTTGAAAGGACCCATGGCCACACCAATTGGAAAAGGCCATCGTTCTTTGAACCTTACCTTGAGAAAGGAACTTAATTTATATGCTAATGTGAGGCCTTGCTACAGCCTACCTGGTTATAAAACTCGATATGATAATGTAAATCTTATCACCATCCGTGAGAACACAGAAGGGGAGTACAGTGGACTTGAACATCAAGTAAGCATCTGTTATTTCTATCAAGGTAATGGAAAACAATCTCAATGTGATTTACATGTAGGCTTTCTTGCGTGCTAGGTGGTGAGAGGTGTGGTTGAAAGCCTTAAGATCATTACCCGTCAAGCAAGTTTACGGGTAGCGGAATATGCTTTTCACTATGCCAAAACTCATGGGAGGGAGAGAGTCTCTGCAATTCACAAAGCCAATATTATGCAGAAAACTGATGGTCTTTTtCTTAAGGTGCAACAGAATTTCAAACTATTAACTCTTCTCCCATATGCACTTTTCACTGGGTGTTGGTGTCCTCATCTAAAGTATTCTTCGTGTCTTCACTTTCAGTGTTGTCGGGAAGTTGCAAAGAAGTATCCTGAGATAAAGTACGAGGAAGTTGTCATTGATAACTGCTGTATGATGGTATGGGTTACTTTATGTTCAATAATCATAGCACTTGTAGGGGAAATTAATTCCTATAGTTGATGAGGAGGTCTGGCAGGGTCAATCTACAACTTCTAGTGTATTCTCCAGAATGTGTTTCTGATAATTAACTGATTTGATGATGAAATTTGGGTTCATAAAAAGTGTTTTCGCAGAATAAAATTTCATTGAATTATGACTTTTGTTATGCAAGTGTAAGTCAGATGGAACTGGTCTTCATTGTGTGTCTACTTTGTGCAGCTTGTGAAGAATCCAGCACTTTTTGATGTATTGGTTATGCCGAATCTTTATGGTGACATTATTAGTGATCTCTGTGCTGGTCTGATTGGAGGATTAGGGTTAACACCAAGGTAATGATGCCTCACTTTCATTTTTtATGGTAAGTTTTCAGATTATCTAACGTCAAAAAACATTATATTATGCAGCTGTAATATTGGTGAGGGAGGTATTGCTCTTGCTGAGGCTGTGCATGGTTCAGCACCGGATATTGCTGGAAAGGTTAGGCTTCATAACTTTAAGATCAATGCCTTGCATGACTGCACGGTCTTTTATTTTCTTTTCTTTGATCACATTTCTCTGATCTAGAACCTCCATTGTTTTGTATGTTACTTGTATGTATTGCTATTATTTATTTATAGGTTTTAAACTATTTCTAGGTTTTAATTTGCATCATATCATAGGGCTTAGCAGTGTCAATTTAGTTCTTGCCCTAATAATTTGTTTCAAATTCTTCTCTTCTATCAATAATCTTGACAAATGTTAACATGTCTTATCAACCTTGGAGTTCGATGTTACGTGACTAATAGATGGCTTTCTGACATGGAATTGCTTGATGTTCATGTGAAAAGAAAaTCATGAGAATATTATTTtATTTATGAAATTAAATGCTTGAGACAGATGGATTACAATTTTGGAATTGCTTGGCTATATTAGATTACAATTTTTCATTTGTTGAATGTTCATCATAAGTTTTGTCTTTAAATTATAGAACCAAGTGAGTATTATTAGTAGGAAATGATATTAAAATTCATTCTATTGTTTAAATTGCAGTTAAAAGTTAAGATTAAAAAAAaCAAAAAaGAACAAAAGTTTGTAGACTTGAGATTAAAAAAAaTGTAGAAAAATGCAGTGCAGAGTAACAAGAGAATGTTTGTTGAAAAACCTCTTCCTAGAGAAAAAAAaaTTGCTGAAATCTCTCAAACTATTTTTTtAAAAAAAAaaTTAAAACAAAAAaGAAGGGATAGAAAACACATGATGAAGAATAACCACTTTTCTCTTAATCGTAGTTTTTTTTtAGAAACCAACTTAACACCCTTGATAGGTACCCCCTATATATCAGAATCAGGAATAAATTTTCTTCAGAGGAAAATGGAGAGCAAAGGCCTGAGGAGCACGTGTAGTGTTCCAATAAAAAACAAAACAACAAGAAAACACATTAGTACAAAATGAATCTCAGGTAGCATTCATGGGAACTTAGATATTTTCCTTGACATTTATCTTCAAATTTCATTCATAGAGTCATTTAGAAGTTTAACTCGCAGTTAAACCTAAAAGTCCATACCAAAACCAAGATAGTACATTCTTTTCTACCTTTTCTAATGAATCCAATTAACAGGAATTCAAATAGGACTTTAAATTCACACACTTTTCCAAAGCTCGATCAATATAAAAGTAATTGACGAGGTTGAATATATGTTTTGATAAATGCACTTTCTTTATTAGAAAGTATTTTTCATGTATCTGAAAACTGGTTAAACTTGCACAGAACATGGCAAATCCAACTGCTTTGCTTTTGAGCGCTGTCACGATGCTTCGACATTTAAAACTCCAGAACAAAGCAGATCGAATCCAAGATGCCATCCTCAACACCATTGCAGAAGGGAAGTATCGAACAGCCGACCTTGGTGGTTCATCATCAACTACCGAGTTCACCAATGCAATCTGTGATCATCTTTGAGGAATGGTGTACATTTGCTTACATTACATTGCGGTATTGTCTCTGAGGAACCTTTGTGGAGGCTTTCCTTGATATCTTTTCATAGTTTCTCTGAAGAGTAATAAGTTCAGATCATATTGATGACTCTGAAGTAGCTTTATCCCAAGGGTGCTACTATTTTTTAACCATTGATCCTTTGCTGGCCATACCAAAGGAAAaCTAAAATGGCCTTGTTCATGTAATCAGCCATACAATGTCAGATGATTGAGATCTGTTAAATTTTATTTCTACGAAAACATGGCTCTTTTTTTTTTTtGTTCCCATTTTTTGGTCTATGTTAGTCTTCATCACTATAGTTTGTAAAAGACGAAAAAGAGCAATCTTATTATCCTTGAAGCTTTGCCATAA

mRNA sequence

TCTTTGATTATTTTTCCTTCAATTTTTCGTCGTCAATCATGGCTTCGCAGCTCCTCAAACGCGCCCTTAGAAGCCAGTCTGGTCAGATCTTCTCCCTTTCATCTAGCCTTAACTCTTCCTCTCCCTTTCCTTTTAGGGCTTTTTCTTCCGCAGCTACTCCGATCCGAGCCACTCTCTTCCCCGGCGATGGTATTGGTCCCGAAATTGCGGAGTCCGTCAAACAGGTGTTTAGAACTGCTGATGTGCCCATCGAATGGGAAGAACACTACGTTGGGGACGAAATAGATCCTCGAACTCAAAGTTTCCTCACATGGGAAAGCTTAGAATCAGTGAGACGTAATGGTGTGGGTTTGAAAGGACCCATGGCCACACCAATTGGAAAAGGCCATCGTTCTTTGAACCTTACCTTGAGAAAGGAACTTAATTTATATGCTAATGTGAGGCCTTGCTACAGCCTACCTGGTTATAAAACTCGATATGATAATGTAAATCTTATCACCATCCGTGAGAACACAGAAGGGGAGTACAGTGGACTTGAACATCAAGTGGTGAGAGGTGTGGTTGAAAGCCTTAAGATCATTACCCGTCAAGCAAGTTTACGGGTAGCGGAATATGCTTTTCACTATGCCAAAACTCATGGGAGGGAGAGAGTCTCTGCAATTCACAAAGCCAATATTATGCAGAAAACTGATGGTCTTTTTCTTAAGTGTTGTCGGGAAGTTGCAAAGAAGTATCCTGAGATAAAGTACGAGGAAGTTGTCATTGATAACTGCTGTATGATGCTTGTGAAGAATCCAGCACTTTTTGATGTATTGGTTATGCCGAATCTTTATGGTGACATTATTAGTGATCTCTGTGCTGGTCTGATTGGAGGATTAGGGTTAACACCAAGCTGTAATATTGGTGAGGGAGGTATTGCTCTTGCTGAGGCTGTGCATGGTTCAGCACCGGATATTGCTGGAAAGAACATGGCAAATCCAACTGCTTTGCTTTTGAGCGCTGTCACGATGCTTCGACATTTAAAACTCCAGAACAAAGCAGATCGAATCCAAGATGCCATCCTCAACACCATTGCAGAAGGGAAGTATCGAACAGCCGACCTTGGTGGTTCATCATCAACTACCGAGTTCACCAATGCAATCTGTGATCATCTTTGAGGAATGGTGTACATTTGCTTACATTACATTGCGGTATTGTCTCTGAGGAACCTTTGTGGAGGCTTTCCTTGATATCTTTTCATAGTTTCTCTGAAGAGTAATAAGTTCAGATCATATTGATGACTCTGAAGTAGCTTTATCCCAAGGGTGCTACTATTTTTTAACCATTGATCCTTTGCTGGCCATACCAAAGGAAAACTAAAATGGCCTTGTTCATGTAATCAGCCATACAATGTCAGATGATTGAGATCTGTTAAATTTTATTTCTACGAAAACATGGCTCTTTTTTTTTTTTGTTCCCATTTTTTGGTCTATGTTAGTCTTCATCACTATAGTTTGTAAAAGACGAAAAAGAGCAATCTTATTATCCTTGAAGCTTTGCCATAA

Coding sequence (CDS)

ATGGCTTCGCAGCTCCTCAAACGCGCCCTTAGAAGCCAGTCTGGTCAGATCTTCTCCCTTTCATCTAGCCTTAACTCTTCCTCTCCCTTTCCTTTTAGGGCTTTTTCTTCCGCAGCTACTCCGATCCGAGCCACTCTCTTCCCCGGCGATGGTATTGGTCCCGAAATTGCGGAGTCCGTCAAACAGGTGTTTAGAACTGCTGATGTGCCCATCGAATGGGAAGAACACTACGTTGGGGACGAAATAGATCCTCGAACTCAAAGTTTCCTCACATGGGAAAGCTTAGAATCAGTGAGACGTAATGGTGTGGGTTTGAAAGGACCCATGGCCACACCAATTGGAAAAGGCCATCGTTCTTTGAACCTTACCTTGAGAAAGGAACTTAATTTATATGCTAATGTGAGGCCTTGCTACAGCCTACCTGGTTATAAAACTCGATATGATAATGTAAATCTTATCACCATCCGTGAGAACACAGAAGGGGAGTACAGTGGACTTGAACATCAAGTGGTGAGAGGTGTGGTTGAAAGCCTTAAGATCATTACCCGTCAAGCAAGTTTACGGGTAGCGGAATATGCTTTTCACTATGCCAAAACTCATGGGAGGGAGAGAGTCTCTGCAATTCACAAAGCCAATATTATGCAGAAAACTGATGGTCTTTTtCTTAAGTGTTGTCGGGAAGTTGCAAAGAAGTATCCTGAGATAAAGTACGAGGAAGTTGTCATTGATAACTGCTGTATGATGCTTGTGAAGAATCCAGCACTTTTTGATGTATTGGTTATGCCGAATCTTTATGGTGACATTATTAGTGATCTCTGTGCTGGTCTGATTGGAGGATTAGGGTTAACACCAAGCTGTAATATTGGTGAGGGAGGTATTGCTCTTGCTGAGGCTGTGCATGGTTCAGCACCGGATATTGCTGGAAAGAACATGGCAAATCCAACTGCTTTGCTTTTGAGCGCTGTCACGATGCTTCGACATTTAAAACTCCAGAACAAAGCAGATCGAATCCAAGATGCCATCCTCAACACCATTGCAGAAGGGAAGTATCGAACAGCCGACCTTGGTGGTTCATCATCAACTACCGAGTTCACCAATGCAATCTGTGATCATCTTTGA

Protein sequence

MASQLLKRALRSQSGQIFSLSSSLNSSSPFPFRAFSSAATPIRATLFPGDGIGPEIAESVKQVFRTADVPIEWEEHYVGDEIDPRTQSFLTWESLESVRRNGVGLKGPMATPIGKGHRSLNLTLRKELNLYANVRPCYSLPGYKTRYDNVNLITIRENTEGEYSGLEHQVVRGVVESLKIITRQASLRVAEYAFHYAKTHGRERVSAIHKANIMQKTDGLFLKCCREVAKKYPEIKYEEVVIDNCCMMLVKNPALFDVLVMPNLYGDIISDLCAGLIGGLGLTPSCNIGEGGIALAEAVHGSAPDIAGKNMANPTALLLSAVTMLRHLKLQNKADRIQDAILNTIAEGKYRTADLGGSSSTTEFTNAICDHL*
BLAST of Cucsa.124520 vs. Swiss-Prot
Match: IDH5_ARATH (Isocitrate dehydrogenase [NAD] catalytic subunit 5, mitochondrial OS=Arabidopsis thaliana GN=IDH5 PE=2 SV=1)

HSP 1 Score: 636.3 bits (1640), Expect = 2.0e-181
Identity = 317/372 (85.22%), Postives = 339/372 (91.13%), Query Frame = 1

Query: 1   MASQLLKRALRSQSGQIFSLSSSLNSSSPFPFRAFSSAATPIRATLFPGDGIGPEIAESV 60
           MA+ L +R + ++S QI    +S + ++    RAF S+ TPI ATLFPGDGIGPEIAESV
Sbjct: 3   MAANLARRLIGNRSTQILGAVNSSSGAASSVARAFCSSTTPITATLFPGDGIGPEIAESV 62

Query: 61  KQVFRTADVPIEWEEHYVGDEIDPRTQSFLTWESLESVRRNGVGLKGPMATPIGKGHRSL 120
           K+VF TA VPIEWEEHYVG EIDPRTQSFLTWESLESVRRN VGLKGPMATPIGKGHRSL
Sbjct: 63  KKVFTTAGVPIEWEEHYVGTEIDPRTQSFLTWESLESVRRNKVGLKGPMATPIGKGHRSL 122

Query: 121 NLTLRKELNLYANVRPCYSLPGYKTRYDNVNLITIRENTEGEYSGLEHQVVRGVVESLKI 180
           NLTLRKELNLYANVRPCYSLPGYKTRYD+V+LITIRENTEGEYSGLEHQVVRGVVESLKI
Sbjct: 123 NLTLRKELNLYANVRPCYSLPGYKTRYDDVDLITIRENTEGEYSGLEHQVVRGVVESLKI 182

Query: 181 ITRQASLRVAEYAFHYAKTHGRERVSAIHKANIMQKTDGLFLKCCREVAKKYPEIKYEEV 240
           ITRQASLRVAEYAF YAKTHGRERVSAIHKANIMQKTDGLFLKCCREVA+KYPEI YEEV
Sbjct: 183 ITRQASLRVAEYAFLYAKTHGRERVSAIHKANIMQKTDGLFLKCCREVAEKYPEITYEEV 242

Query: 241 VIDNCCMMLVKNPALFDVLVMPNLYGDIISDLCAGLIGGLGLTPSCNIGEGGIALAEAVH 300
           VIDNCCMMLVKNPALFDVLVMPNLYGDIISDLCAGL+GGLGLTPSCNIGE G+ALAEAVH
Sbjct: 243 VIDNCCMMLVKNPALFDVLVMPNLYGDIISDLCAGLVGGLGLTPSCNIGEDGVALAEAVH 302

Query: 301 GSAPDIAGKNMANPTALLLSAVTMLRHLKLQNKADRIQDAILNTIAEGKYRTADLGGSSS 360
           GSAPDIAGKN+ANPTALLLS V MLRHLK   +A++I  AI+NTIAEGKYRTADLGGSS+
Sbjct: 303 GSAPDIAGKNLANPTALLLSGVMMLRHLKFNEQAEQIHSAIINTIAEGKYRTADLGGSST 362

Query: 361 TTEFTNAICDHL 373
           TTEFT AICDHL
Sbjct: 363 TTEFTKAICDHL 374

BLAST of Cucsa.124520 vs. Swiss-Prot
Match: IDH6_ARATH (Isocitrate dehydrogenase [NAD] catalytic subunit 6, mitochondrial OS=Arabidopsis thaliana GN=IDH6 PE=2 SV=2)

HSP 1 Score: 600.5 bits (1547), Expect = 1.2e-170
Identity = 302/374 (80.75%), Postives = 334/374 (89.30%), Query Frame = 1

Query: 1   MASQLLKRALRSQSGQIFSLSSSLNSSSPF--PFRAFSSAATPIRATLFPGDGIGPEIAE 60
           M + L +R + + S QI   SSS  SS PF    RAF S++TPI+ATLFPGDGIGPEIAE
Sbjct: 3   MTAFLARRLIGNGSSQILGTSSS--SSGPFISVSRAFFSSSTPIKATLFPGDGIGPEIAE 62

Query: 61  SVKQVFRTADVPIEWEEHYVGDEIDPRTQSFLTWESLESVRRNGVGLKGPMATPIGKGHR 120
           SVKQVF  ADV I+W+E +VG E+DPRT SFLTW++L+SV +N VGLKGPMATPIGKGHR
Sbjct: 63  SVKQVFTAADVVIDWDEQFVGTEVDPRTNSFLTWDNLQSVLKNKVGLKGPMATPIGKGHR 122

Query: 121 SLNLTLRKELNLYANVRPCYSLPGYKTRYDNVNLITIRENTEGEYSGLEHQVVRGVVESL 180
           SLNLTLRKELNLYANVRPCYSLPGYKTRYD+V+LITIRENTEGEYSGLEHQVV+GVVESL
Sbjct: 123 SLNLTLRKELNLYANVRPCYSLPGYKTRYDDVDLITIRENTEGEYSGLEHQVVKGVVESL 182

Query: 181 KIITRQASLRVAEYAFHYAKTHGRERVSAIHKANIMQKTDGLFLKCCREVAKKYPEIKYE 240
           KIITR+AS+RVAEYAF YAKTHGR++VSAIHKANIMQKTDGLFL+CC EVA KYPEI YE
Sbjct: 183 KIITRKASMRVAEYAFLYAKTHGRKKVSAIHKANIMQKTDGLFLQCCDEVAAKYPEIYYE 242

Query: 241 EVVIDNCCMMLVKNPALFDVLVMPNLYGDIISDLCAGLIGGLGLTPSCNIGEGGIALAEA 300
           +VVIDNCCMMLVKNPALFDVLVMPNLYGDIISDLCAGL+GGLGLTPS NIGE GIALAEA
Sbjct: 243 KVVIDNCCMMLVKNPALFDVLVMPNLYGDIISDLCAGLVGGLGLTPSMNIGEDGIALAEA 302

Query: 301 VHGSAPDIAGKNMANPTALLLSAVTMLRHLKLQNKADRIQDAILNTIAEGKYRTADLGGS 360
           VHGSAPDIAG N+ANPTALLLS V MLRHLKL  +A++I  AI+NTIAEGKYRTADLGGS
Sbjct: 303 VHGSAPDIAGMNLANPTALLLSGVMMLRHLKLNKQAEQIHSAIINTIAEGKYRTADLGGS 362

Query: 361 SSTTEFTNAICDHL 373
           S+TT+FT AICDHL
Sbjct: 363 STTTDFTKAICDHL 374

BLAST of Cucsa.124520 vs. Swiss-Prot
Match: LEU3_SOLTU (3-isopropylmalate dehydrogenase, chloroplastic OS=Solanum tuberosum PE=2 SV=1)

HSP 1 Score: 597.0 bits (1538), Expect = 1.4e-169
Identity = 303/351 (86.32%), Postives = 321/351 (91.45%), Query Frame = 1

Query: 1   MASQLLKRALRSQSGQIFSLSSSLNSSSPFPFRAFSSAATP--IRATLFPGDGIGPEIAE 60
           MA Q+ KR LR ++        S+ SS  F  R F+S +    IRATLFPGDGIGPEIAE
Sbjct: 1   MALQIAKRLLRCRA-------DSVASSVRFFDRTFTSESNSNLIRATLFPGDGIGPEIAE 60

Query: 61  SVKQVFRTADVPIEWEEHYVGDEIDPRTQSFLTWESLESVRRNGVGLKGPMATPIGKGHR 120
           SV+Q+F+ A+VPIEWEEHYVG E+DPRT SFLTWESLESVRRN VGLKGPMATPIGKGHR
Sbjct: 61  SVRQIFKVAEVPIEWEEHYVGTEVDPRTNSFLTWESLESVRRNKVGLKGPMATPIGKGHR 120

Query: 121 SLNLTLRKELNLYANVRPCYSLPGYKTRYDNVNLITIRENTEGEYSGLEHQVVRGVVESL 180
           SLNLTLRKELNLYANVRPCYSLPGYKTRYD+VNLITIRENTEGEYSGLEHQVVRGVVESL
Sbjct: 121 SLNLTLRKELNLYANVRPCYSLPGYKTRYDDVNLITIRENTEGEYSGLEHQVVRGVVESL 180

Query: 181 KIITRQASLRVAEYAFHYAKTHGRERVSAIHKANIMQKTDGLFLKCCREVAKKYPEIKYE 240
           KIITRQASLRVAEYAFHYAKTHGRERVSAIHKANIMQKTDGLFLKCCREVA+KYPEIKYE
Sbjct: 181 KIITRQASLRVAEYAFHYAKTHGRERVSAIHKANIMQKTDGLFLKCCREVAEKYPEIKYE 240

Query: 241 EVVIDNCCMMLVKNPALFDVLVMPNLYGDIISDLCAGLIGGLGLTPSCNIGEGGIALAEA 300
           EVVIDNCCMMLVKNPALFDVLVMPNLYGDIISDLCAGLIGGLGLTPSCNIGEGGIALAEA
Sbjct: 241 EVVIDNCCMMLVKNPALFDVLVMPNLYGDIISDLCAGLIGGLGLTPSCNIGEGGIALAEA 300

Query: 301 VHGSAPDIAGKNMANPTALLLSAVTMLRHLKLQNKADRIQDAILNTIAEGK 350
           VHGSAPDIAGKN+ANPTALLLS+V+MLRHL+L +KADRIQDAIL TIA GK
Sbjct: 301 VHGSAPDIAGKNLANPTALLLSSVSMLRHLELHDKADRIQDAILKTIAGGK 344

BLAST of Cucsa.124520 vs. Swiss-Prot
Match: IDH3A_DROME (Probable isocitrate dehydrogenase [NAD] subunit alpha, mitochondrial OS=Drosophila melanogaster GN=l(1)G0156 PE=2 SV=1)

HSP 1 Score: 427.2 bits (1097), Expect = 1.9e-118
Identity = 208/347 (59.94%), Postives = 272/347 (78.39%), Query Frame = 1

Query: 26  SSSPFPFRAFSSAATPIRATLFPGDGIGPEIAESVKQVFRTADVPIEWEEHYVGDEIDPR 85
           +++P   R++SS    +  TL PGDGIGPEI+ +V+++F  A+VPIEWE   V     P 
Sbjct: 34  NATPAASRSYSSGTKKV--TLIPGDGIGPEISAAVQKIFTAANVPIEWEAVDVTPVRGPD 93

Query: 86  TQSFLTWESLESVRRNGVGLKGPMATPIGKGHRSLNLTLRKELNLYANVRPCYSLPGYKT 145
            +  +   +++SV  N +GLKGP+ TP+GKGHRSLNL LRKE NLYANVRPC SL GYKT
Sbjct: 94  GKFGIPQAAIDSVNTNKIGLKGPLMTPVGKGHRSLNLALRKEFNLYANVRPCRSLEGYKT 153

Query: 146 RYDNVNLITIRENTEGEYSGLEHQVVRGVVESLKIITRQASLRVAEYAFHYAKTHGRERV 205
            YD+V+++TIRENTEGEYSG+EH++V GVV+S+K+IT +AS RVAEYAF YAK + R++V
Sbjct: 154 LYDDVDVVTIRENTEGEYSGIEHEIVDGVVQSIKLITEEASKRVAEYAFQYAKNNNRKKV 213

Query: 206 SAIHKANIMQKTDGLFLKCCREVAKKYPEIKYEEVVIDNCCMMLVKNPALFDVLVMPNLY 265
           + +HKANIM+ +DGLFL+C R++A+K+PEI++EE  +D  C+ +V+NP  +DVLVMPNLY
Sbjct: 214 TVVHKANIMRMSDGLFLRCVRDMAQKFPEIQFEEKYLDTVCLNMVQNPGKYDVLVMPNLY 273

Query: 266 GDIISDLCAGLIGGLGLTPSCNIGEGGIALAEAVHGSAPDIAGKNMANPTALLLSAVTML 325
           GDI+SD+CAGL+GGLGLTPS N+G  G AL E+VHG+APDIAGK++ANPTALLLSAV ML
Sbjct: 274 GDILSDMCAGLVGGLGLTPSGNMGLNG-ALFESVHGTAPDIAGKDLANPTALLLSAVMML 333

Query: 326 RHLKLQNKADRIQDAILNTIAEGKYRTADLGGSSSTTEFTNAICDHL 373
           RH++L   AD+I+ A   TI EGKY T DLGG +  +EFTN IC  L
Sbjct: 334 RHMELNTYADKIERAAFETIKEGKYLTGDLGGRAKCSEFTNEICAKL 377

BLAST of Cucsa.124520 vs. Swiss-Prot
Match: IDHA_DICDI (Isocitrate dehydrogenase [NAD] regulatory subunit A, mitochondrial OS=Dictyostelium discoideum GN=idhA PE=3 SV=1)

HSP 1 Score: 424.5 bits (1090), Expect = 1.2e-117
Identity = 206/337 (61.13%), Postives = 268/337 (79.53%), Query Frame = 1

Query: 33  RAFSSAATPI-RATLFPGDGIGPEIAESVKQVFRTADVPIEWEEHYVGDEIDPRTQSFLT 92
           R +SS+ + I + TL PGDGIGPEI+ESVK+VF     PIEWE   V         + ++
Sbjct: 14  RNYSSSTSKIQKVTLIPGDGIGPEISESVKRVFSAVKAPIEWETVVVD------ANTGIS 73

Query: 93  WESLESVRRNGVGLKGPMATPIGKGHRSLNLTLRKELNLYANVRPCYSLPGYKTRYDNVN 152
            E +ES+ +N +GLKGP++TPIG GH+SLNL LRK  NLYAN+RPC S+PG+KTRY+NVN
Sbjct: 74  KEVIESISKNKIGLKGPISTPIGTGHQSLNLGLRKTFNLYANIRPCLSIPGHKTRYNNVN 133

Query: 153 LITIRENTEGEYSGLEHQVVRGVVESLKIITRQASLRVAEYAFHYAKTHGRERVSAIHKA 212
            + +RENTEGEYSG+E+Q V+GV +S+KIIT++AS R+A YAF YA  +GR++V+ IHKA
Sbjct: 134 TVVVRENTEGEYSGIENQPVKGVAQSIKIITKEASTRIAHYAFQYALANGRKKVTCIHKA 193

Query: 213 NIMQKTDGLFLKCCREVAKKYPEIKYEEVVIDNCCMMLVKNPALFDVLVMPNLYGDIISD 272
           NIM+++DGLF+K CREV+ +YP IKYEE+ IDN CM LV +P   DV+V+PNLYGDI+SD
Sbjct: 194 NIMKQSDGLFVKSCREVSTRYPSIKYEELTIDNNCMQLVLDPNQMDVMVLPNLYGDIVSD 253

Query: 273 LCAGLIGGLGLTPSCNIGEGGIALAEAVHGSAPDIAGKNMANPTALLLSAVTMLRHLKLQ 332
           LCAGLIGGLGLTPS NIGE G A+ EAVHG+APDIAGKN ANPTAL+LS++ MLRHL   
Sbjct: 254 LCAGLIGGLGLTPSGNIGENGSAIFEAVHGTAPDIAGKNKANPTALILSSIMMLRHLGHF 313

Query: 333 NKADRIQDAILNTIAEGKYRTADLGGSSSTTEFTNAI 369
           ++A  I++A+LNT+ EGK +T DLGG+SS +E+T+ +
Sbjct: 314 HEASIIENAVLNTLTEGKVKTGDLGGNSSCSEYTDEL 344

BLAST of Cucsa.124520 vs. TrEMBL
Match: A0A0A0KF41_CUCSA (Isocitrate dehydrogenase [NAD] subunit, mitochondrial OS=Cucumis sativus GN=Csa_6G446300 PE=3 SV=1)

HSP 1 Score: 742.3 bits (1915), Expect = 2.9e-211
Identity = 372/372 (100.00%), Postives = 372/372 (100.00%), Query Frame = 1

Query: 1   MASQLLKRALRSQSGQIFSLSSSLNSSSPFPFRAFSSAATPIRATLFPGDGIGPEIAESV 60
           MASQLLKRALRSQSGQIFSLSSSLNSSSPFPFRAFSSAATPIRATLFPGDGIGPEIAESV
Sbjct: 1   MASQLLKRALRSQSGQIFSLSSSLNSSSPFPFRAFSSAATPIRATLFPGDGIGPEIAESV 60

Query: 61  KQVFRTADVPIEWEEHYVGDEIDPRTQSFLTWESLESVRRNGVGLKGPMATPIGKGHRSL 120
           KQVFRTADVPIEWEEHYVGDEIDPRTQSFLTWESLESVRRNGVGLKGPMATPIGKGHRSL
Sbjct: 61  KQVFRTADVPIEWEEHYVGDEIDPRTQSFLTWESLESVRRNGVGLKGPMATPIGKGHRSL 120

Query: 121 NLTLRKELNLYANVRPCYSLPGYKTRYDNVNLITIRENTEGEYSGLEHQVVRGVVESLKI 180
           NLTLRKELNLYANVRPCYSLPGYKTRYDNVNLITIRENTEGEYSGLEHQVVRGVVESLKI
Sbjct: 121 NLTLRKELNLYANVRPCYSLPGYKTRYDNVNLITIRENTEGEYSGLEHQVVRGVVESLKI 180

Query: 181 ITRQASLRVAEYAFHYAKTHGRERVSAIHKANIMQKTDGLFLKCCREVAKKYPEIKYEEV 240
           ITRQASLRVAEYAFHYAKTHGRERVSAIHKANIMQKTDGLFLKCCREVAKKYPEIKYEEV
Sbjct: 181 ITRQASLRVAEYAFHYAKTHGRERVSAIHKANIMQKTDGLFLKCCREVAKKYPEIKYEEV 240

Query: 241 VIDNCCMMLVKNPALFDVLVMPNLYGDIISDLCAGLIGGLGLTPSCNIGEGGIALAEAVH 300
           VIDNCCMMLVKNPALFDVLVMPNLYGDIISDLCAGLIGGLGLTPSCNIGEGGIALAEAVH
Sbjct: 241 VIDNCCMMLVKNPALFDVLVMPNLYGDIISDLCAGLIGGLGLTPSCNIGEGGIALAEAVH 300

Query: 301 GSAPDIAGKNMANPTALLLSAVTMLRHLKLQNKADRIQDAILNTIAEGKYRTADLGGSSS 360
           GSAPDIAGKNMANPTALLLSAVTMLRHLKLQNKADRIQDAILNTIAEGKYRTADLGGSSS
Sbjct: 301 GSAPDIAGKNMANPTALLLSAVTMLRHLKLQNKADRIQDAILNTIAEGKYRTADLGGSSS 360

Query: 361 TTEFTNAICDHL 373
           TTEFTNAICDHL
Sbjct: 361 TTEFTNAICDHL 372

BLAST of Cucsa.124520 vs. TrEMBL
Match: W9SLH0_9ROSA (Isocitrate dehydrogenase [NAD] subunit, mitochondrial OS=Morus notabilis GN=L484_024368 PE=3 SV=1)

HSP 1 Score: 661.0 bits (1704), Expect = 8.6e-187
Identity = 337/376 (89.63%), Postives = 353/376 (93.88%), Query Frame = 1

Query: 1   MASQLLKRALRSQSGQIFSL---SSSLNSSSPFPFRAFSSAATP-IRATLFPGDGIGPEI 60
           MASQLL+RAL S+S QI S    SSSL+S +    RAFSS+A   IRATLFPGDGIGPEI
Sbjct: 3   MASQLLRRALGSRSSQIISSANPSSSLSSPATATARAFSSSAADLIRATLFPGDGIGPEI 62

Query: 61  AESVKQVFRTADVPIEWEEHYVGDEIDPRTQSFLTWESLESVRRNGVGLKGPMATPIGKG 120
           AESVKQVFR A+VPIEWEEHYVGD+IDPRTQSFLTW+SLESVR N VGLKGPMATPIGKG
Sbjct: 63  AESVKQVFREAEVPIEWEEHYVGDQIDPRTQSFLTWDSLESVRVNKVGLKGPMATPIGKG 122

Query: 121 HRSLNLTLRKELNLYANVRPCYSLPGYKTRYDNVNLITIRENTEGEYSGLEHQVVRGVVE 180
           HRSLNLTLRKELNLYANVRPCYSLPGYKTRYD+VNLITIRENTEGEYSGLEHQVVRGVVE
Sbjct: 123 HRSLNLTLRKELNLYANVRPCYSLPGYKTRYDDVNLITIRENTEGEYSGLEHQVVRGVVE 182

Query: 181 SLKIITRQASLRVAEYAFHYAKTHGRERVSAIHKANIMQKTDGLFLKCCREVAKKYPEIK 240
           SLKIITRQASLRVAEYAFHYAK HGRERVSAIHKANIMQKTDGLFLKCCREVA+KYPEIK
Sbjct: 183 SLKIITRQASLRVAEYAFHYAKMHGRERVSAIHKANIMQKTDGLFLKCCREVAEKYPEIK 242

Query: 241 YEEVVIDNCCMMLVKNPALFDVLVMPNLYGDIISDLCAGLIGGLGLTPSCNIGEGGIALA 300
           YEEVVIDNCCMMLVKNPALFDVLVMPNLYGDIISDLCAGLIGGLGLTPSCNIGEGGIALA
Sbjct: 243 YEEVVIDNCCMMLVKNPALFDVLVMPNLYGDIISDLCAGLIGGLGLTPSCNIGEGGIALA 302

Query: 301 EAVHGSAPDIAGKNMANPTALLLSAVTMLRHLKLQNKADRIQDAILNTIAEGKYRTADLG 360
           EAVHGSAPDIAGKN+ANPTALLLS+VTMLRHL+L +KA+RIQ AILNTIAEGKYRTADLG
Sbjct: 303 EAVHGSAPDIAGKNLANPTALLLSSVTMLRHLELHDKAERIQAAILNTIAEGKYRTADLG 362

Query: 361 GSSSTTEFTNAICDHL 373
           G SSTT+FT AICDHL
Sbjct: 363 GKSSTTDFTKAICDHL 378

BLAST of Cucsa.124520 vs. TrEMBL
Match: M5VYP8_PRUPE (Isocitrate dehydrogenase [NAD] subunit, mitochondrial OS=Prunus persica GN=PRUPE_ppa007185mg PE=3 SV=1)

HSP 1 Score: 661.0 bits (1704), Expect = 8.6e-187
Identity = 334/379 (88.13%), Postives = 357/379 (94.20%), Query Frame = 1

Query: 1   MASQLLKRALRSQSGQIFSLSSS-----LNSSSPFP--FRAFSSAATPIRATLFPGDGIG 60
           MAS LL+RAL S+S Q  S +++     L+SSSP     R FSS +TPIRATLFPGDGIG
Sbjct: 1   MASHLLRRALGSRSTQFLSGAANPSPSLLSSSSPSSQAARVFSSDSTPIRATLFPGDGIG 60

Query: 61  PEIAESVKQVFRTADVPIEWEEHYVGDEIDPRTQSFLTWESLESVRRNGVGLKGPMATPI 120
           PEIAESVKQVFR A+VPIEWEEHYVGD+IDPRTQSFLTWESLESVR+N VGLKGPMATPI
Sbjct: 61  PEIAESVKQVFREAEVPIEWEEHYVGDQIDPRTQSFLTWESLESVRKNRVGLKGPMATPI 120

Query: 121 GKGHRSLNLTLRKELNLYANVRPCYSLPGYKTRYDNVNLITIRENTEGEYSGLEHQVVRG 180
           GKGHRSLNLTLRKELNLYANVRPCYSLPGYKTRYD+VNLITIRENTEGEYSGLEHQVVRG
Sbjct: 121 GKGHRSLNLTLRKELNLYANVRPCYSLPGYKTRYDDVNLITIRENTEGEYSGLEHQVVRG 180

Query: 181 VVESLKIITRQASLRVAEYAFHYAKTHGRERVSAIHKANIMQKTDGLFLKCCREVAKKYP 240
           VVESLKIITRQASLRVAEYAFHYAKTHGRERVSAIHKANIMQKTDGLFLKCCREVA+KYP
Sbjct: 181 VVESLKIITRQASLRVAEYAFHYAKTHGRERVSAIHKANIMQKTDGLFLKCCREVAEKYP 240

Query: 241 EIKYEEVVIDNCCMMLVKNPALFDVLVMPNLYGDIISDLCAGLIGGLGLTPSCNIGEGGI 300
           +IKYEEVVIDNCCMMLVKNPALFDVLVMPNLYGDIISDLCAGLIGGLGLTPSCNIGEGGI
Sbjct: 241 DIKYEEVVIDNCCMMLVKNPALFDVLVMPNLYGDIISDLCAGLIGGLGLTPSCNIGEGGI 300

Query: 301 ALAEAVHGSAPDIAGKNMANPTALLLSAVTMLRHLKLQNKADRIQDAILNTIAEGKYRTA 360
           ALAEAVHGSAPDIAGKN+ANPTALLLS+VTMLRHL+L +KADRIQ+AIL+TIAEGK+RTA
Sbjct: 301 ALAEAVHGSAPDIAGKNLANPTALLLSSVTMLRHLELHDKADRIQNAILSTIAEGKFRTA 360

Query: 361 DLGGSSSTTEFTNAICDHL 373
           DLGG+SST++FT AICDHL
Sbjct: 361 DLGGASSTSDFTKAICDHL 379

BLAST of Cucsa.124520 vs. TrEMBL
Match: A0A097QH91_9ROSA (Isocitrate dehydrogenase [NAD] subunit, mitochondrial OS=Prunus sibirica PE=2 SV=1)

HSP 1 Score: 661.0 bits (1704), Expect = 8.6e-187
Identity = 334/379 (88.13%), Postives = 357/379 (94.20%), Query Frame = 1

Query: 1   MASQLLKRALRSQSGQIFSLSSS-----LNSSSPFP--FRAFSSAATPIRATLFPGDGIG 60
           MAS LL+RAL S+S Q  S +++     L+SSSP     R FSS +TPIRATLFPGDGIG
Sbjct: 1   MASHLLRRALGSRSTQFISGAANPSPSLLSSSSPSSQAARVFSSDSTPIRATLFPGDGIG 60

Query: 61  PEIAESVKQVFRTADVPIEWEEHYVGDEIDPRTQSFLTWESLESVRRNGVGLKGPMATPI 120
           PEIAESVKQVFR A+VPIEWEEHYVGD+IDPRTQSFLTWESLESVR+N VGLKGPMATPI
Sbjct: 61  PEIAESVKQVFREAEVPIEWEEHYVGDQIDPRTQSFLTWESLESVRKNRVGLKGPMATPI 120

Query: 121 GKGHRSLNLTLRKELNLYANVRPCYSLPGYKTRYDNVNLITIRENTEGEYSGLEHQVVRG 180
           GKGHRSLNLTLRKELNLYANVRPCYSLPGYKTRYD+VNLITIRENTEGEYSGLEHQVVRG
Sbjct: 121 GKGHRSLNLTLRKELNLYANVRPCYSLPGYKTRYDDVNLITIRENTEGEYSGLEHQVVRG 180

Query: 181 VVESLKIITRQASLRVAEYAFHYAKTHGRERVSAIHKANIMQKTDGLFLKCCREVAKKYP 240
           VVESLKIITRQASLRVAEYAFHYAKTHGRERVSAIHKANIMQKTDGLFLKCCREVA+KYP
Sbjct: 181 VVESLKIITRQASLRVAEYAFHYAKTHGRERVSAIHKANIMQKTDGLFLKCCREVAEKYP 240

Query: 241 EIKYEEVVIDNCCMMLVKNPALFDVLVMPNLYGDIISDLCAGLIGGLGLTPSCNIGEGGI 300
           +IKYEEVVIDNCCMMLVKNPALFDVLVMPNLYGDIISDLCAGLIGGLGLTPSCNIGEGGI
Sbjct: 241 DIKYEEVVIDNCCMMLVKNPALFDVLVMPNLYGDIISDLCAGLIGGLGLTPSCNIGEGGI 300

Query: 301 ALAEAVHGSAPDIAGKNMANPTALLLSAVTMLRHLKLQNKADRIQDAILNTIAEGKYRTA 360
           ALAEAVHGSAPDIAGKN+ANPTALLLS+VTMLRHL+L +KADRIQ+AIL+TIAEGK+RTA
Sbjct: 301 ALAEAVHGSAPDIAGKNLANPTALLLSSVTMLRHLELHDKADRIQNAILSTIAEGKFRTA 360

Query: 361 DLGGSSSTTEFTNAICDHL 373
           DLGG+SST++FT AICDHL
Sbjct: 361 DLGGASSTSDFTKAICDHL 379

BLAST of Cucsa.124520 vs. TrEMBL
Match: A0A067E9Z4_CITSI (Isocitrate dehydrogenase [NAD] subunit, mitochondrial OS=Citrus sinensis GN=CISIN_1g017413mg PE=3 SV=1)

HSP 1 Score: 659.1 bits (1699), Expect = 3.3e-186
Identity = 332/373 (89.01%), Postives = 351/373 (94.10%), Query Frame = 1

Query: 1   MASQLLKRALRSQSGQIFSLSSSLNSSSPFPF-RAFSSAATPIRATLFPGDGIGPEIAES 60
           MA+QLL+R L S+S QI S ++  N +   P  RAFSS  TPI ATLFPGDGIGPEIAES
Sbjct: 1   MATQLLRRVLGSRSRQILSTTNP-NPNLALPIARAFSSDITPITATLFPGDGIGPEIAES 60

Query: 61  VKQVFRTADVPIEWEEHYVGDEIDPRTQSFLTWESLESVRRNGVGLKGPMATPIGKGHRS 120
           VKQVFRTA+VP+EWEEHYVG EIDPRTQSFLTWESLESVRRN VGLKGPMATPIGKGHRS
Sbjct: 61  VKQVFRTAEVPVEWEEHYVGTEIDPRTQSFLTWESLESVRRNKVGLKGPMATPIGKGHRS 120

Query: 121 LNLTLRKELNLYANVRPCYSLPGYKTRYDNVNLITIRENTEGEYSGLEHQVVRGVVESLK 180
           LNLTLRKELNLYANVRPCYSLPGYKTRYD+VNLITIRENTEGEYSGLEHQVVRGVVESLK
Sbjct: 121 LNLTLRKELNLYANVRPCYSLPGYKTRYDDVNLITIRENTEGEYSGLEHQVVRGVVESLK 180

Query: 181 IITRQASLRVAEYAFHYAKTHGRERVSAIHKANIMQKTDGLFLKCCREVAKKYPEIKYEE 240
           IITRQASLRVAEYAFHYAKTHGRERVSAIHKANIMQKTDGLFLKCCREVA+KYPEI YEE
Sbjct: 181 IITRQASLRVAEYAFHYAKTHGRERVSAIHKANIMQKTDGLFLKCCREVAEKYPEITYEE 240

Query: 241 VVIDNCCMMLVKNPALFDVLVMPNLYGDIISDLCAGLIGGLGLTPSCNIGEGGIALAEAV 300
           VVIDNCCMMLVKNPA FDVLVMPNLYGDIISDLCAGLIGGLGLTPSCNIGEGGIALAEAV
Sbjct: 241 VVIDNCCMMLVKNPAAFDVLVMPNLYGDIISDLCAGLIGGLGLTPSCNIGEGGIALAEAV 300

Query: 301 HGSAPDIAGKNMANPTALLLSAVTMLRHLKLQNKADRIQDAILNTIAEGKYRTADLGGSS 360
           HGSAPDIAGKN+ANPTALLLS+VTMLRHL+L +KADRIQ+AIL+TIAEGKYRTADLGGSS
Sbjct: 301 HGSAPDIAGKNLANPTALLLSSVTMLRHLELHDKADRIQNAILSTIAEGKYRTADLGGSS 360

Query: 361 STTEFTNAICDHL 373
           +T++FT AICDHL
Sbjct: 361 TTSDFTKAICDHL 372

BLAST of Cucsa.124520 vs. TAIR10
Match: AT5G03290.1 (AT5G03290.1 isocitrate dehydrogenase V)

HSP 1 Score: 636.3 bits (1640), Expect = 1.2e-182
Identity = 317/372 (85.22%), Postives = 339/372 (91.13%), Query Frame = 1

Query: 1   MASQLLKRALRSQSGQIFSLSSSLNSSSPFPFRAFSSAATPIRATLFPGDGIGPEIAESV 60
           MA+ L +R + ++S QI    +S + ++    RAF S+ TPI ATLFPGDGIGPEIAESV
Sbjct: 3   MAANLARRLIGNRSTQILGAVNSSSGAASSVARAFCSSTTPITATLFPGDGIGPEIAESV 62

Query: 61  KQVFRTADVPIEWEEHYVGDEIDPRTQSFLTWESLESVRRNGVGLKGPMATPIGKGHRSL 120
           K+VF TA VPIEWEEHYVG EIDPRTQSFLTWESLESVRRN VGLKGPMATPIGKGHRSL
Sbjct: 63  KKVFTTAGVPIEWEEHYVGTEIDPRTQSFLTWESLESVRRNKVGLKGPMATPIGKGHRSL 122

Query: 121 NLTLRKELNLYANVRPCYSLPGYKTRYDNVNLITIRENTEGEYSGLEHQVVRGVVESLKI 180
           NLTLRKELNLYANVRPCYSLPGYKTRYD+V+LITIRENTEGEYSGLEHQVVRGVVESLKI
Sbjct: 123 NLTLRKELNLYANVRPCYSLPGYKTRYDDVDLITIRENTEGEYSGLEHQVVRGVVESLKI 182

Query: 181 ITRQASLRVAEYAFHYAKTHGRERVSAIHKANIMQKTDGLFLKCCREVAKKYPEIKYEEV 240
           ITRQASLRVAEYAF YAKTHGRERVSAIHKANIMQKTDGLFLKCCREVA+KYPEI YEEV
Sbjct: 183 ITRQASLRVAEYAFLYAKTHGRERVSAIHKANIMQKTDGLFLKCCREVAEKYPEITYEEV 242

Query: 241 VIDNCCMMLVKNPALFDVLVMPNLYGDIISDLCAGLIGGLGLTPSCNIGEGGIALAEAVH 300
           VIDNCCMMLVKNPALFDVLVMPNLYGDIISDLCAGL+GGLGLTPSCNIGE G+ALAEAVH
Sbjct: 243 VIDNCCMMLVKNPALFDVLVMPNLYGDIISDLCAGLVGGLGLTPSCNIGEDGVALAEAVH 302

Query: 301 GSAPDIAGKNMANPTALLLSAVTMLRHLKLQNKADRIQDAILNTIAEGKYRTADLGGSSS 360
           GSAPDIAGKN+ANPTALLLS V MLRHLK   +A++I  AI+NTIAEGKYRTADLGGSS+
Sbjct: 303 GSAPDIAGKNLANPTALLLSGVMMLRHLKFNEQAEQIHSAIINTIAEGKYRTADLGGSST 362

Query: 361 TTEFTNAICDHL 373
           TTEFT AICDHL
Sbjct: 363 TTEFTKAICDHL 374

BLAST of Cucsa.124520 vs. TAIR10
Match: AT3G09810.1 (AT3G09810.1 isocitrate dehydrogenase VI)

HSP 1 Score: 600.5 bits (1547), Expect = 7.0e-172
Identity = 302/374 (80.75%), Postives = 334/374 (89.30%), Query Frame = 1

Query: 1   MASQLLKRALRSQSGQIFSLSSSLNSSSPF--PFRAFSSAATPIRATLFPGDGIGPEIAE 60
           M + L +R + + S QI   SSS  SS PF    RAF S++TPI+ATLFPGDGIGPEIAE
Sbjct: 3   MTAFLARRLIGNGSSQILGTSSS--SSGPFISVSRAFFSSSTPIKATLFPGDGIGPEIAE 62

Query: 61  SVKQVFRTADVPIEWEEHYVGDEIDPRTQSFLTWESLESVRRNGVGLKGPMATPIGKGHR 120
           SVKQVF  ADV I+W+E +VG E+DPRT SFLTW++L+SV +N VGLKGPMATPIGKGHR
Sbjct: 63  SVKQVFTAADVVIDWDEQFVGTEVDPRTNSFLTWDNLQSVLKNKVGLKGPMATPIGKGHR 122

Query: 121 SLNLTLRKELNLYANVRPCYSLPGYKTRYDNVNLITIRENTEGEYSGLEHQVVRGVVESL 180
           SLNLTLRKELNLYANVRPCYSLPGYKTRYD+V+LITIRENTEGEYSGLEHQVV+GVVESL
Sbjct: 123 SLNLTLRKELNLYANVRPCYSLPGYKTRYDDVDLITIRENTEGEYSGLEHQVVKGVVESL 182

Query: 181 KIITRQASLRVAEYAFHYAKTHGRERVSAIHKANIMQKTDGLFLKCCREVAKKYPEIKYE 240
           KIITR+AS+RVAEYAF YAKTHGR++VSAIHKANIMQKTDGLFL+CC EVA KYPEI YE
Sbjct: 183 KIITRKASMRVAEYAFLYAKTHGRKKVSAIHKANIMQKTDGLFLQCCDEVAAKYPEIYYE 242

Query: 241 EVVIDNCCMMLVKNPALFDVLVMPNLYGDIISDLCAGLIGGLGLTPSCNIGEGGIALAEA 300
           +VVIDNCCMMLVKNPALFDVLVMPNLYGDIISDLCAGL+GGLGLTPS NIGE GIALAEA
Sbjct: 243 KVVIDNCCMMLVKNPALFDVLVMPNLYGDIISDLCAGLVGGLGLTPSMNIGEDGIALAEA 302

Query: 301 VHGSAPDIAGKNMANPTALLLSAVTMLRHLKLQNKADRIQDAILNTIAEGKYRTADLGGS 360
           VHGSAPDIAG N+ANPTALLLS V MLRHLKL  +A++I  AI+NTIAEGKYRTADLGGS
Sbjct: 303 VHGSAPDIAGMNLANPTALLLSGVMMLRHLKLNKQAEQIHSAIINTIAEGKYRTADLGGS 362

Query: 361 SSTTEFTNAICDHL 373
           S+TT+FT AICDHL
Sbjct: 363 STTTDFTKAICDHL 374

BLAST of Cucsa.124520 vs. TAIR10
Match: AT4G35260.1 (AT4G35260.1 isocitrate dehydrogenase 1)

HSP 1 Score: 331.6 bits (849), Expect = 6.1e-91
Identity = 169/331 (51.06%), Postives = 231/331 (69.79%), Query Frame = 1

Query: 41  PIRATLFPGDGIGPEIAESVKQVFRTADVPIEWEEHYVGDEIDPRTQSFLTWESLESVRR 100
           P   TL PGDGIGP +  +V+QV      PI +E++ V  E+     S +  E +ES+R+
Sbjct: 37  PRAVTLIPGDGIGPLVTNAVEQVMEAMHAPIFFEKYDVHGEM-----SRVPPEVMESIRK 96

Query: 101 NGVGLKGPMATPIGKGHRSLNLTLRKELNLYANVRPCYSLPGYKTRYDNVNLITIRENTE 160
           N V LKG + TP+G G  SLN+ LRKEL+L+A++  C++LPG  TR++NV+++ IRENTE
Sbjct: 97  NKVCLKGGLKTPVGGGVSSLNVQLRKELDLFASLVNCFNLPGLPTRHENVDIVVIRENTE 156

Query: 161 GEYSGLEHQVVRGVVESLKIITRQASLRVAEYAFHYAKTHGRERVSAIHKANIMQKTDGL 220
           GEY+GLEH+VV GVVESLK+IT+  S R+A+YAF YA  + R++V+A+HKANIM+  DGL
Sbjct: 157 GEYAGLEHEVVPGVVESLKVITKFCSERIAKYAFEYAYLNNRKKVTAVHKANIMKLADGL 216

Query: 221 FLKCCREVAKKYPEIKYEEVVIDNCCMMLVKNPALFDVLVMPNLYGDIISDLCAGLIGGL 280
           FL+ CREVAKKYP I Y E+++DNCCM LV  P  FDV+V PNLYG+++++  AG+ GG 
Sbjct: 217 FLESCREVAKKYPSITYNEIIVDNCCMQLVAKPEQFDVMVTPNLYGNLVANTAAGIAGGT 276

Query: 281 GLTPSCNIGEGGIAL---AEAVHGSAPDIAGKNMANPTALLLSAVTMLRHLKLQNKADRI 340
           G+ P  N+G         A A +     I  +N ANP ALLLS+  MLRHL+  + ADR+
Sbjct: 277 GVMPGGNVGADHAVFEQGASAGNVGKDKIVLENKANPVALLLSSAMMLRHLQFPSFADRL 336

Query: 341 QDAILNTIAEGKYRTADLGGSSSTTEFTNAI 369
           + A+   IAEGK RT DLGG+S+T E  +A+
Sbjct: 337 ETAVKKVIAEGKCRTKDLGGTSTTQEVVDAV 362

BLAST of Cucsa.124520 vs. TAIR10
Match: AT4G35650.1 (AT4G35650.1 isocitrate dehydrogenase III)

HSP 1 Score: 322.8 bits (826), Expect = 2.8e-88
Identity = 172/362 (47.51%), Postives = 237/362 (65.47%), Query Frame = 1

Query: 19  SLSSSLNSSSPFPFRAFSSAAT---------PIRATLFPGDGIGPEIAESVKQVFRTADV 78
           S+ + L ++ P PF + S + T         P   TL PGDGIGP +  +V+QV      
Sbjct: 7   SIFNRLLANPPSPFTSLSRSITYMPRPGDGAPRTVTLIPGDGIGPLVTGAVEQVMEAMHA 66

Query: 79  PIEWEEHYVGDEIDPRTQSFLTWESLESVRRNGVGLKGPMATPIGKGHRSLNLTLRKELN 138
           P+ +E + V   +    +     E +ESV+RN V LKG +ATP+G G  SLN+ LRKEL+
Sbjct: 67  PVHFERYEVLGNMRKVPE-----EVIESVKRNKVCLKGGLATPVGGGVSSLNMQLRKELD 126

Query: 139 LYANVRPCYSLPGYKTRYDNVNLITIRENTEGEYSGLEHQVVRGVVESLKIITRQASLRV 198
           ++A++  C ++PG  TR++NV+++ IRENTEGEYSGLEH+VV GVVESLK+IT+  S R+
Sbjct: 127 IFASLVNCINVPGLVTRHENVDIVVIRENTEGEYSGLEHEVVPGVVESLKVITKFCSERI 186

Query: 199 AEYAFHYAKTHGRERVSAIHKANIMQKTDGLFLKCCREVAKKYPEIKYEEVVIDNCCMML 258
           A YAF YA  + R++V+A+HKANIM+  DGLFL+ CREVAK Y  I Y E+++DNCCM L
Sbjct: 187 ARYAFEYAYLNNRKKVTAVHKANIMKLADGLFLESCREVAKHYSGITYNEIIVDNCCMQL 246

Query: 259 VKNPALFDVLVMPNLYGDIISDLCAGLIGGLGLTPSCNIG-EGGIALAEAVHGSAPD--I 318
           V  P  FDV+V PNLYG++I++  AG+ GG G+ P  N+G E  I    A  G+  +  +
Sbjct: 247 VAKPEQFDVMVTPNLYGNLIANTAAGIAGGTGVMPGGNVGAEHAIFEQGASAGNVGNDKM 306

Query: 319 AGKNMANPTALLLSAVTMLRHLKLQNKADRIQDAILNTIAEGKYRTADLGGSSSTTEFTN 369
             +  ANP ALLLS+  MLRHL+    ADR++ A+   I EGKYRT DLGG  +T E  +
Sbjct: 307 VEQKKANPVALLLSSAMMLRHLRFPTFADRLETAVKQVIKEGKYRTKDLGGDCTTQEVVD 363

BLAST of Cucsa.124520 vs. TAIR10
Match: AT2G17130.1 (AT2G17130.1 isocitrate dehydrogenase subunit 2)

HSP 1 Score: 319.3 bits (817), Expect = 3.1e-87
Identity = 162/338 (47.93%), Postives = 229/338 (67.75%), Query Frame = 1

Query: 41  PIRATLFPGDGIGPEIAESVKQVFRTADVPIEWEEHYVGDEIDPRTQSFLTWESLESVRR 100
           P   TL PGDG+GP +  +V+QV      P+ +E   V  ++    +  L     ES+++
Sbjct: 37  PRPVTLIPGDGVGPLVTNAVQQVMEAMHAPVYFEPFEVHGDMKSLPEGLL-----ESIKK 96

Query: 101 NGVGLKGPMATPIGKGHRSLNLTLRKELNLYANVRPCYSLPGYKTRYDNVNLITIRENTE 160
           N V LKG + TP+G G  SLN+ LRKEL+L+A++  C++LPG  +R++NV+++ IRENTE
Sbjct: 97  NKVCLKGGLKTPVGGGVSSLNVNLRKELDLFASLVNCFNLPGLASRHENVDIVVIRENTE 156

Query: 161 GEYSGLEHQVVRGVVESLKIITRQASLRVAEYAFHYAKTHGRERVSAIHKANIMQKTDGL 220
           GEY+GLEH+VV GVVESLK+IT+  S R+A+YAF YA  + R++V+A+HKANIM+  DGL
Sbjct: 157 GEYAGLEHEVVPGVVESLKVITKFCSERIAKYAFEYAYLNNRKKVTAVHKANIMKLADGL 216

Query: 221 FLKCCREVAKKYPEIKYEEVVIDNCCMMLVKNPALFDVLVMPNLYGDIISDLCAGLIGGL 280
           FL+ C+EVAKKYP I Y E+++DNCCM LV  P  FDV+V PNLYG+++++  AG+ GG 
Sbjct: 217 FLESCQEVAKKYPSIAYNEIIVDNCCMQLVARPEQFDVMVTPNLYGNLVANTAAGIAGGT 276

Query: 281 GLTPSCNIGEGGIALAEAVHGSAPDIAGKNM------ANPTALLLSAVTMLRHLKLQNKA 340
           G+ P  N+   G   A    G++    GK+       ANP ALLLS+  MLRHL+  + A
Sbjct: 277 GVMPGGNV---GAEYAVFEQGASAGNVGKDTTEEQKNANPVALLLSSAMMLRHLQFPSFA 336

Query: 341 DRIQDAILNTIAEGKYRTADLGGSSSTTEFTNAICDHL 373
           DR++ A+   IAEG  RT DLGG+S+T E  +A+  +L
Sbjct: 337 DRLETAVKRVIAEGNCRTEDLGGNSTTQEVVDAVIANL 366

BLAST of Cucsa.124520 vs. NCBI nr
Match: gi|449451022|ref|XP_004143261.1| (PREDICTED: isocitrate dehydrogenase [NAD])

HSP 1 Score: 742.3 bits (1915), Expect = 4.2e-211
Identity = 372/372 (100.00%), Postives = 372/372 (100.00%), Query Frame = 1

Query: 1   MASQLLKRALRSQSGQIFSLSSSLNSSSPFPFRAFSSAATPIRATLFPGDGIGPEIAESV 60
           MASQLLKRALRSQSGQIFSLSSSLNSSSPFPFRAFSSAATPIRATLFPGDGIGPEIAESV
Sbjct: 1   MASQLLKRALRSQSGQIFSLSSSLNSSSPFPFRAFSSAATPIRATLFPGDGIGPEIAESV 60

Query: 61  KQVFRTADVPIEWEEHYVGDEIDPRTQSFLTWESLESVRRNGVGLKGPMATPIGKGHRSL 120
           KQVFRTADVPIEWEEHYVGDEIDPRTQSFLTWESLESVRRNGVGLKGPMATPIGKGHRSL
Sbjct: 61  KQVFRTADVPIEWEEHYVGDEIDPRTQSFLTWESLESVRRNGVGLKGPMATPIGKGHRSL 120

Query: 121 NLTLRKELNLYANVRPCYSLPGYKTRYDNVNLITIRENTEGEYSGLEHQVVRGVVESLKI 180
           NLTLRKELNLYANVRPCYSLPGYKTRYDNVNLITIRENTEGEYSGLEHQVVRGVVESLKI
Sbjct: 121 NLTLRKELNLYANVRPCYSLPGYKTRYDNVNLITIRENTEGEYSGLEHQVVRGVVESLKI 180

Query: 181 ITRQASLRVAEYAFHYAKTHGRERVSAIHKANIMQKTDGLFLKCCREVAKKYPEIKYEEV 240
           ITRQASLRVAEYAFHYAKTHGRERVSAIHKANIMQKTDGLFLKCCREVAKKYPEIKYEEV
Sbjct: 181 ITRQASLRVAEYAFHYAKTHGRERVSAIHKANIMQKTDGLFLKCCREVAKKYPEIKYEEV 240

Query: 241 VIDNCCMMLVKNPALFDVLVMPNLYGDIISDLCAGLIGGLGLTPSCNIGEGGIALAEAVH 300
           VIDNCCMMLVKNPALFDVLVMPNLYGDIISDLCAGLIGGLGLTPSCNIGEGGIALAEAVH
Sbjct: 241 VIDNCCMMLVKNPALFDVLVMPNLYGDIISDLCAGLIGGLGLTPSCNIGEGGIALAEAVH 300

Query: 301 GSAPDIAGKNMANPTALLLSAVTMLRHLKLQNKADRIQDAILNTIAEGKYRTADLGGSSS 360
           GSAPDIAGKNMANPTALLLSAVTMLRHLKLQNKADRIQDAILNTIAEGKYRTADLGGSSS
Sbjct: 301 GSAPDIAGKNMANPTALLLSAVTMLRHLKLQNKADRIQDAILNTIAEGKYRTADLGGSSS 360

Query: 361 TTEFTNAICDHL 373
           TTEFTNAICDHL
Sbjct: 361 TTEFTNAICDHL 372

BLAST of Cucsa.124520 vs. NCBI nr
Match: gi|659096690|ref|XP_008449234.1| (PREDICTED: isocitrate dehydrogenase [NAD])

HSP 1 Score: 729.6 bits (1882), Expect = 2.8e-207
Identity = 365/372 (98.12%), Postives = 369/372 (99.19%), Query Frame = 1

Query: 1   MASQLLKRALRSQSGQIFSLSSSLNSSSPFPFRAFSSAATPIRATLFPGDGIGPEIAESV 60
           MASQLLKRALRSQSGQIFSLSS+L SSSPFPFRAFSSA TPIRATLFPGDGIGPEIA SV
Sbjct: 1   MASQLLKRALRSQSGQIFSLSSNLTSSSPFPFRAFSSATTPIRATLFPGDGIGPEIAASV 60

Query: 61  KQVFRTADVPIEWEEHYVGDEIDPRTQSFLTWESLESVRRNGVGLKGPMATPIGKGHRSL 120
           KQVFRTADVPIEWEEHYVGDEIDPRTQSFLTWESLESVRRNGVGLKGPMATPIGKGHRSL
Sbjct: 61  KQVFRTADVPIEWEEHYVGDEIDPRTQSFLTWESLESVRRNGVGLKGPMATPIGKGHRSL 120

Query: 121 NLTLRKELNLYANVRPCYSLPGYKTRYDNVNLITIRENTEGEYSGLEHQVVRGVVESLKI 180
           NLTLRKELNLYANVRPCYSLPGYKTRYDNVNLITIRENTEGEYSGLEHQVVRGVVESLKI
Sbjct: 121 NLTLRKELNLYANVRPCYSLPGYKTRYDNVNLITIRENTEGEYSGLEHQVVRGVVESLKI 180

Query: 181 ITRQASLRVAEYAFHYAKTHGRERVSAIHKANIMQKTDGLFLKCCREVAKKYPEIKYEEV 240
           ITRQASLRVAEYAFHYAKTHGRERVSAIHKANIMQKTDGLFLKCCREVAKKYPEIKYEEV
Sbjct: 181 ITRQASLRVAEYAFHYAKTHGRERVSAIHKANIMQKTDGLFLKCCREVAKKYPEIKYEEV 240

Query: 241 VIDNCCMMLVKNPALFDVLVMPNLYGDIISDLCAGLIGGLGLTPSCNIGEGGIALAEAVH 300
           VIDNCCMMLVKNPALFDVLVMPNLYGDIISDLCAGLIGGLGLTPSCNIGEGGIALAEAVH
Sbjct: 241 VIDNCCMMLVKNPALFDVLVMPNLYGDIISDLCAGLIGGLGLTPSCNIGEGGIALAEAVH 300

Query: 301 GSAPDIAGKNMANPTALLLSAVTMLRHLKLQNKADRIQDAILNTIAEGKYRTADLGGSSS 360
           GSAPDIAGKNMANPTALLLSAVTMLRHL+LQ+KADRIQDAILNTIAEGKYRTADLGGSSS
Sbjct: 301 GSAPDIAGKNMANPTALLLSAVTMLRHLELQDKADRIQDAILNTIAEGKYRTADLGGSSS 360

Query: 361 TTEFTNAICDHL 373
           TTEFT+AICDHL
Sbjct: 361 TTEFTDAICDHL 372

BLAST of Cucsa.124520 vs. NCBI nr
Match: gi|703141489|ref|XP_010107515.1| (Isocitrate dehydrogenase [NAD])

HSP 1 Score: 661.0 bits (1704), Expect = 1.2e-186
Identity = 337/376 (89.63%), Postives = 353/376 (93.88%), Query Frame = 1

Query: 1   MASQLLKRALRSQSGQIFSL---SSSLNSSSPFPFRAFSSAATP-IRATLFPGDGIGPEI 60
           MASQLL+RAL S+S QI S    SSSL+S +    RAFSS+A   IRATLFPGDGIGPEI
Sbjct: 3   MASQLLRRALGSRSSQIISSANPSSSLSSPATATARAFSSSAADLIRATLFPGDGIGPEI 62

Query: 61  AESVKQVFRTADVPIEWEEHYVGDEIDPRTQSFLTWESLESVRRNGVGLKGPMATPIGKG 120
           AESVKQVFR A+VPIEWEEHYVGD+IDPRTQSFLTW+SLESVR N VGLKGPMATPIGKG
Sbjct: 63  AESVKQVFREAEVPIEWEEHYVGDQIDPRTQSFLTWDSLESVRVNKVGLKGPMATPIGKG 122

Query: 121 HRSLNLTLRKELNLYANVRPCYSLPGYKTRYDNVNLITIRENTEGEYSGLEHQVVRGVVE 180
           HRSLNLTLRKELNLYANVRPCYSLPGYKTRYD+VNLITIRENTEGEYSGLEHQVVRGVVE
Sbjct: 123 HRSLNLTLRKELNLYANVRPCYSLPGYKTRYDDVNLITIRENTEGEYSGLEHQVVRGVVE 182

Query: 181 SLKIITRQASLRVAEYAFHYAKTHGRERVSAIHKANIMQKTDGLFLKCCREVAKKYPEIK 240
           SLKIITRQASLRVAEYAFHYAK HGRERVSAIHKANIMQKTDGLFLKCCREVA+KYPEIK
Sbjct: 183 SLKIITRQASLRVAEYAFHYAKMHGRERVSAIHKANIMQKTDGLFLKCCREVAEKYPEIK 242

Query: 241 YEEVVIDNCCMMLVKNPALFDVLVMPNLYGDIISDLCAGLIGGLGLTPSCNIGEGGIALA 300
           YEEVVIDNCCMMLVKNPALFDVLVMPNLYGDIISDLCAGLIGGLGLTPSCNIGEGGIALA
Sbjct: 243 YEEVVIDNCCMMLVKNPALFDVLVMPNLYGDIISDLCAGLIGGLGLTPSCNIGEGGIALA 302

Query: 301 EAVHGSAPDIAGKNMANPTALLLSAVTMLRHLKLQNKADRIQDAILNTIAEGKYRTADLG 360
           EAVHGSAPDIAGKN+ANPTALLLS+VTMLRHL+L +KA+RIQ AILNTIAEGKYRTADLG
Sbjct: 303 EAVHGSAPDIAGKNLANPTALLLSSVTMLRHLELHDKAERIQAAILNTIAEGKYRTADLG 362

Query: 361 GSSSTTEFTNAICDHL 373
           G SSTT+FT AICDHL
Sbjct: 363 GKSSTTDFTKAICDHL 378

BLAST of Cucsa.124520 vs. NCBI nr
Match: gi|700284253|gb|AIU64850.1| (isocitrate dehydrogenase [Prunus sibirica])

HSP 1 Score: 661.0 bits (1704), Expect = 1.2e-186
Identity = 334/379 (88.13%), Postives = 357/379 (94.20%), Query Frame = 1

Query: 1   MASQLLKRALRSQSGQIFSLSSS-----LNSSSPFP--FRAFSSAATPIRATLFPGDGIG 60
           MAS LL+RAL S+S Q  S +++     L+SSSP     R FSS +TPIRATLFPGDGIG
Sbjct: 1   MASHLLRRALGSRSTQFISGAANPSPSLLSSSSPSSQAARVFSSDSTPIRATLFPGDGIG 60

Query: 61  PEIAESVKQVFRTADVPIEWEEHYVGDEIDPRTQSFLTWESLESVRRNGVGLKGPMATPI 120
           PEIAESVKQVFR A+VPIEWEEHYVGD+IDPRTQSFLTWESLESVR+N VGLKGPMATPI
Sbjct: 61  PEIAESVKQVFREAEVPIEWEEHYVGDQIDPRTQSFLTWESLESVRKNRVGLKGPMATPI 120

Query: 121 GKGHRSLNLTLRKELNLYANVRPCYSLPGYKTRYDNVNLITIRENTEGEYSGLEHQVVRG 180
           GKGHRSLNLTLRKELNLYANVRPCYSLPGYKTRYD+VNLITIRENTEGEYSGLEHQVVRG
Sbjct: 121 GKGHRSLNLTLRKELNLYANVRPCYSLPGYKTRYDDVNLITIRENTEGEYSGLEHQVVRG 180

Query: 181 VVESLKIITRQASLRVAEYAFHYAKTHGRERVSAIHKANIMQKTDGLFLKCCREVAKKYP 240
           VVESLKIITRQASLRVAEYAFHYAKTHGRERVSAIHKANIMQKTDGLFLKCCREVA+KYP
Sbjct: 181 VVESLKIITRQASLRVAEYAFHYAKTHGRERVSAIHKANIMQKTDGLFLKCCREVAEKYP 240

Query: 241 EIKYEEVVIDNCCMMLVKNPALFDVLVMPNLYGDIISDLCAGLIGGLGLTPSCNIGEGGI 300
           +IKYEEVVIDNCCMMLVKNPALFDVLVMPNLYGDIISDLCAGLIGGLGLTPSCNIGEGGI
Sbjct: 241 DIKYEEVVIDNCCMMLVKNPALFDVLVMPNLYGDIISDLCAGLIGGLGLTPSCNIGEGGI 300

Query: 301 ALAEAVHGSAPDIAGKNMANPTALLLSAVTMLRHLKLQNKADRIQDAILNTIAEGKYRTA 360
           ALAEAVHGSAPDIAGKN+ANPTALLLS+VTMLRHL+L +KADRIQ+AIL+TIAEGK+RTA
Sbjct: 301 ALAEAVHGSAPDIAGKNLANPTALLLSSVTMLRHLELHDKADRIQNAILSTIAEGKFRTA 360

Query: 361 DLGGSSSTTEFTNAICDHL 373
           DLGG+SST++FT AICDHL
Sbjct: 361 DLGGASSTSDFTKAICDHL 379

BLAST of Cucsa.124520 vs. NCBI nr
Match: gi|595825122|ref|XP_007205355.1| (hypothetical protein PRUPE_ppa007185mg [Prunus persica])

HSP 1 Score: 661.0 bits (1704), Expect = 1.2e-186
Identity = 334/379 (88.13%), Postives = 357/379 (94.20%), Query Frame = 1

Query: 1   MASQLLKRALRSQSGQIFSLSSS-----LNSSSPFP--FRAFSSAATPIRATLFPGDGIG 60
           MAS LL+RAL S+S Q  S +++     L+SSSP     R FSS +TPIRATLFPGDGIG
Sbjct: 1   MASHLLRRALGSRSTQFLSGAANPSPSLLSSSSPSSQAARVFSSDSTPIRATLFPGDGIG 60

Query: 61  PEIAESVKQVFRTADVPIEWEEHYVGDEIDPRTQSFLTWESLESVRRNGVGLKGPMATPI 120
           PEIAESVKQVFR A+VPIEWEEHYVGD+IDPRTQSFLTWESLESVR+N VGLKGPMATPI
Sbjct: 61  PEIAESVKQVFREAEVPIEWEEHYVGDQIDPRTQSFLTWESLESVRKNRVGLKGPMATPI 120

Query: 121 GKGHRSLNLTLRKELNLYANVRPCYSLPGYKTRYDNVNLITIRENTEGEYSGLEHQVVRG 180
           GKGHRSLNLTLRKELNLYANVRPCYSLPGYKTRYD+VNLITIRENTEGEYSGLEHQVVRG
Sbjct: 121 GKGHRSLNLTLRKELNLYANVRPCYSLPGYKTRYDDVNLITIRENTEGEYSGLEHQVVRG 180

Query: 181 VVESLKIITRQASLRVAEYAFHYAKTHGRERVSAIHKANIMQKTDGLFLKCCREVAKKYP 240
           VVESLKIITRQASLRVAEYAFHYAKTHGRERVSAIHKANIMQKTDGLFLKCCREVA+KYP
Sbjct: 181 VVESLKIITRQASLRVAEYAFHYAKTHGRERVSAIHKANIMQKTDGLFLKCCREVAEKYP 240

Query: 241 EIKYEEVVIDNCCMMLVKNPALFDVLVMPNLYGDIISDLCAGLIGGLGLTPSCNIGEGGI 300
           +IKYEEVVIDNCCMMLVKNPALFDVLVMPNLYGDIISDLCAGLIGGLGLTPSCNIGEGGI
Sbjct: 241 DIKYEEVVIDNCCMMLVKNPALFDVLVMPNLYGDIISDLCAGLIGGLGLTPSCNIGEGGI 300

Query: 301 ALAEAVHGSAPDIAGKNMANPTALLLSAVTMLRHLKLQNKADRIQDAILNTIAEGKYRTA 360
           ALAEAVHGSAPDIAGKN+ANPTALLLS+VTMLRHL+L +KADRIQ+AIL+TIAEGK+RTA
Sbjct: 301 ALAEAVHGSAPDIAGKNLANPTALLLSSVTMLRHLELHDKADRIQNAILSTIAEGKFRTA 360

Query: 361 DLGGSSSTTEFTNAICDHL 373
           DLGG+SST++FT AICDHL
Sbjct: 361 DLGGASSTSDFTKAICDHL 379

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
IDH5_ARATH2.0e-18185.22Isocitrate dehydrogenase [NAD] catalytic subunit 5, mitochondrial OS=Arabidopsis... [more]
IDH6_ARATH1.2e-17080.75Isocitrate dehydrogenase [NAD] catalytic subunit 6, mitochondrial OS=Arabidopsis... [more]
LEU3_SOLTU1.4e-16986.323-isopropylmalate dehydrogenase, chloroplastic OS=Solanum tuberosum PE=2 SV=1[more]
IDH3A_DROME1.9e-11859.94Probable isocitrate dehydrogenase [NAD] subunit alpha, mitochondrial OS=Drosophi... [more]
IDHA_DICDI1.2e-11761.13Isocitrate dehydrogenase [NAD] regulatory subunit A, mitochondrial OS=Dictyostel... [more]
Match NameE-valueIdentityDescription
A0A0A0KF41_CUCSA2.9e-211100.00Isocitrate dehydrogenase [NAD] subunit, mitochondrial OS=Cucumis sativus GN=Csa_... [more]
W9SLH0_9ROSA8.6e-18789.63Isocitrate dehydrogenase [NAD] subunit, mitochondrial OS=Morus notabilis GN=L484... [more]
M5VYP8_PRUPE8.6e-18788.13Isocitrate dehydrogenase [NAD] subunit, mitochondrial OS=Prunus persica GN=PRUPE... [more]
A0A097QH91_9ROSA8.6e-18788.13Isocitrate dehydrogenase [NAD] subunit, mitochondrial OS=Prunus sibirica PE=2 SV... [more]
A0A067E9Z4_CITSI3.3e-18689.01Isocitrate dehydrogenase [NAD] subunit, mitochondrial OS=Citrus sinensis GN=CISI... [more]
Match NameE-valueIdentityDescription
AT5G03290.11.2e-18285.22 isocitrate dehydrogenase V[more]
AT3G09810.17.0e-17280.75 isocitrate dehydrogenase VI[more]
AT4G35260.16.1e-9151.06 isocitrate dehydrogenase 1[more]
AT4G35650.12.8e-8847.51 isocitrate dehydrogenase III[more]
AT2G17130.13.1e-8747.93 isocitrate dehydrogenase subunit 2[more]
Match NameE-valueIdentityDescription
gi|449451022|ref|XP_004143261.1|4.2e-211100.00PREDICTED: isocitrate dehydrogenase [NAD][more]
gi|659096690|ref|XP_008449234.1|2.8e-20798.12PREDICTED: isocitrate dehydrogenase [NAD][more]
gi|703141489|ref|XP_010107515.1|1.2e-18689.63Isocitrate dehydrogenase [NAD][more]
gi|700284253|gb|AIU64850.1|1.2e-18688.13isocitrate dehydrogenase [Prunus sibirica][more]
gi|595825122|ref|XP_007205355.1|1.2e-18688.13hypothetical protein PRUPE_ppa007185mg [Prunus persica][more]
The following terms have been associated with this gene:
Vocabulary: INTERPRO
TermDefinition
IPR001804Isocitrate and isopropylmalate dehydrogenases family
IPR004434Isocitrate_DH_NAD
IPR019818IsoCit/isopropylmalate_DH_CS
IPR024084IsoPropMal-DH-like_dom
IPR001804Isocitrate and isopropylmalate dehydrogenases family
IPR019818IsoCit/isopropylmalate_DH_CS
IPR024084IsoPropMal-DH-like_dom
IPR001804Isocitrate and isopropylmalate dehydrogenases family
IPR019818IsoCit/isopropylmalate_DH_CS
IPR024084IsoPropMal-DH-like_dom
Vocabulary: Molecular Function
TermDefinition
GO:0016616oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor
GO:0000287magnesium ion binding
GO:0051287NAD binding
GO:0004449isocitrate dehydrogenase (NAD+) activity
GO:0016616oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor
GO:0000287magnesium ion binding
GO:0051287NAD binding
GO:0016616oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor
GO:0000287magnesium ion binding
GO:0051287NAD binding
Vocabulary: Biological Process
TermDefinition
GO:0055114oxidation-reduction process
GO:0006099tricarboxylic acid cycle
GO:0055114oxidation-reduction process
GO:0055114oxidation-reduction process
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006099 tricarboxylic acid cycle
biological_process GO:0055114 oxidation-reduction process
cellular_component GO:0005739 mitochondrion
cellular_component GO:0005575 cellular_component
molecular_function GO:0004449 isocitrate dehydrogenase (NAD+) activity
molecular_function GO:0000287 magnesium ion binding
molecular_function GO:0051287 NAD binding
molecular_function GO:0016616 oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Cucsa.124520.1Cucsa.124520.1mRNA
Cucsa.124520.2Cucsa.124520.2mRNA
Cucsa.124520.3Cucsa.124520.3mRNA


Analysis Name: InterPro Annotations of cucumber (Gy14)
Date Performed: 2017-01-17
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001804Isocitrate and isopropylmalate dehydrogenases familyPANTHERPTHR11835DECARBOXYLATING DEHYDROGENASES-ISOCITRATE, ISOPROPYLMALATE, TARTRATEcoord: 41..284
score: 1.1E
IPR019818Isocitrate/isopropylmalate dehydrogenase, conserved sitePROSITEPS00470IDH_IMDHcoord: 263..282
scor
IPR024084Isopropylmalate dehydrogenase-like domainGENE3DG3DSA:3.40.718.10coord: 43..284
score: 1.1
IPR024084Isopropylmalate dehydrogenase-like domainPFAMPF00180Iso_dhcoord: 45..284
score: 9.0
IPR024084Isopropylmalate dehydrogenase-like domainSMARTSM01329Iso_dh_2coord: 43..285
score: 8.7
NoneNo IPR availableunknownSSF53659Isocitrate/Isopropylmalate dehydrogenase-likecoord: 42..284
score: 3.09

The following gene(s) are paralogous to this gene:

None

The following block(s) are covering this gene:
GeneOrganismBlock
Cucsa.124520Cucumber (Chinese Long) v3cgycucB170
Cucsa.124520Cucumber (Chinese Long) v3cgycucB172
Cucsa.124520Watermelon (97103) v2cgywmbB192
Cucsa.124520Watermelon (97103) v2cgywmbB194
Cucsa.124520Wax gourdcgywgoB224
Cucsa.124520Cucumber (Gy14) v1cgycgyB073
Cucsa.124520Cucumber (Gy14) v1cgycgyB074
Cucsa.124520Cucumber (Gy14) v1cgycgyB075
Cucsa.124520Cucurbita maxima (Rimu)cgycmaB0325
Cucsa.124520Cucurbita maxima (Rimu)cgycmaB0329
Cucsa.124520Cucurbita moschata (Rifu)cgycmoB0320
Cucsa.124520Cucurbita moschata (Rifu)cgycmoB0321
Cucsa.124520Cucurbita moschata (Rifu)cgycmoB0325
Cucsa.124520Wild cucumber (PI 183967)cgycpiB169
Cucsa.124520Wild cucumber (PI 183967)cgycpiB170
Cucsa.124520Wild cucumber (PI 183967)cgycpiB167
Cucsa.124520Cucumber (Chinese Long) v2cgycuB168
Cucsa.124520Cucumber (Chinese Long) v2cgycuB166
Cucsa.124520Melon (DHL92) v3.5.1cgymeB190
Cucsa.124520Melon (DHL92) v3.5.1cgymeB191
Cucsa.124520Watermelon (Charleston Gray)cgywcgB196
Cucsa.124520Watermelon (Charleston Gray)cgywcgB198
Cucsa.124520Watermelon (97103) v1cgywmB199
Cucsa.124520Watermelon (97103) v1cgywmB200
Cucsa.124520Watermelon (97103) v1cgywmB203
Cucsa.124520Cucurbita pepo (Zucchini)cgycpeB0316
Cucsa.124520Cucurbita pepo (Zucchini)cgycpeB0318
Cucsa.124520Cucurbita pepo (Zucchini)cgycpeB0320
Cucsa.124520Bottle gourd (USVL1VR-Ls)cgylsiB191
Cucsa.124520Melon (DHL92) v3.6.1cgymedB189
Cucsa.124520Melon (DHL92) v3.6.1cgymedB190
Cucsa.124520Silver-seed gourdcarcgyB0189
Cucsa.124520Silver-seed gourdcarcgyB0292