Cucsa.123170 (gene) Cucumber (Gy14) v1

NameCucsa.123170
Typegene
OrganismCucumis sativus (Cucumber (Gy14) v1)
DescriptionUbiquitin conjugating enzyme, putative
Locationscaffold01000 : 465723 .. 471080 (+)
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
TTTATTTTTATATACTTCTTCTCACTTCTCCCTATTCTTTTTtACTTCTTATTTCCTTCTTTGTCTCTTCCTATTCTTCTTCTCTAAATCATCCGATTGTTGTTTTTTtCTCGCTATGTCATCAGGAAACGAGAACTATCATCTGGTTCCGCTTCTGGGTTTGTGAAATCCCACCACCCGATGATCAGACGCTCTTCCAAAATGCTAAAAAACAAAGGGGTATAACTATAATTTTGGTTTCTATCTCTCTATTTCTATGTGTGATTGTTTTGTTTTTGTTCAACACTTTCCGCTTGATCCATGTTTATGGTTTGATCATCGAGTGTGATGTACCTCATTTTTGTATCACTTTTTTGGGTTGAGTCAATTTCAAACTTTTTCCAACTTTTGCTACTGGCCCACTACGGATGATTGAAATTTATGAGTTGGGTATTGttttttttTTTtAGTGTAGTGTTTGTGAAATCTTGTTTGGAAATTCGGGAATTACGATCGTGTTTTATATCTGGAAGGTGTTTGTTGAACTGGAATTAGCGCTGCAGTTTTCATGCCACATTGTGTTGTCTTTAAATTGATTTTCTTCCATTTAGTATCGTTGCATGATTGGATAGTTATCAATTCTGTGAACTGAGTGGAAGTGACGCTTGATTGTTGCTTTATATTGTAGTTTTCTCTTATTTGGAGCACTAACTGGTCTTGAATTATTAGAAGCCAATTATGAGAGACATCTAGCGTATTGAAAACTCTTAAAAGTCTATTTCGATTGTGCTTTGGTCAGATGAAAGTCATCAGCAAAGTGATATTTCTTGCTGTTGTTGTTAGCTCTCATATTTTTCATATTCTAATTCTTTTTCACTTACATTCACTGACACAGAAAGGGAAAAGATCCAAATTTTATAATGTGATGGTTGCATTTGAATTGGAATTCTTTCGATGAGTGAAAAATTGACCATAATATTAGAGTGACAGGGAGTGAAGTGTCAGTCAATAAGTAGCGCTGAAAAATTGTATGAGATTGAGCAATGGTATTGTTTTGCACCAGTAAATAATCTTCTATGCCAGTGCCCAATGTGTTTATACAAAATAAACTTTAGATTTGTATGGTTTTATTCTTGTCTATAACTAAATATATCTTGTGTGTTATTCTGATGCTCTTTTGACATCTTGCATTGTCTGGACATTCATCTGGTTAGCTATGCTGCTGGAAGAAGGCATGCCCTGCAATGTCCCCTTTTGTTTCATACTTTCATCTTTTCTTCTTTCGTTTTTtCTACTCATGATCTATTTCCGAAAATAAAACTTTTCTTTTTTCTACTCATGCCACTGCATTTTATACACATAATTTAGATTGATCACCTTGGTTGTTCAATGAAGGATTGAAGCACAAAACTACCCATTCTGATATCTTTATTTGTTCTTCCCCTTCTAGGTTATTTCACTCGATGTGATTGATGTAGACAAAGATGAAGCTTCCGATGCCATGTTTATTCACCAGAAGTTTGATGCAAATGACAAAGGAAAGGGTATATATCACCATGGTGATCATCAATTGAAGGTTCGTTATCACAGTATTTGTTCATTGCTTGTTTGGTGTCTTCTTTCACATCTGTTTTCCACTTTTCTTTTCTTATGGAAACAGAGGAAATGTGATTTACTTTAAGATGTTACAAAGAAATGAAAGGATATGGCATCCTCATGTAAAGTACGAGATACAATATCACCTACTGTATATGCTAGCAAAAAACCAATGTCTAAAGATTAATTATTTCTGAAACAATTACCCTGCAACTTCAGCCCTTTTTAAATACCGTCTACTTCTTCTAACCTAATTATTCAATAAATTATATAAACCACACCTTGCCACTTGCCAGAATGTTACTTTTTTTCCTCTTtTTTtCCCCAAATAACATTAAATACTTGCTTTCGAAGATCTATTTACAAAATGTGTGATCCAGTGTCTTGTTAAGGTCAAGCTTATTGACATGTTACGTTTTTGAAATCTGTATTCACATTTTTTtCTTTTTAAAAGAACCACTTGCCTTGTTCTTTTTAGAAAAAACTATAGGTGAAGTAAATTAGTATAAAACAACAATAAACTACAATCGCCTACACATGTTTTTATTATAGTTTGATCCAAAATATGAGAATTTTATTTTTCCCTAATGGCTCTAAAACAATCTGAAGCCCATATTTGGTTTTGATGATCAAACCCTATGAACAGAAGAGTTCCAACAAATTTTTTATAACTTTTGACCTAATTCACTTTTTTTAAAAACATACTCAAGGTATAAGCACCGAAACCATGCTTTAGTTGAAGTTTAACGACACTTTCTTTGATAATTAGGAGTCGTTGAAGATGTAAGTTTTGTAAATTGAAACAGACATGGTATGCTTGCGATACCTGTTCTAATTATTCACAATTATAGCTAAAGTTTTTACTTTGTTCAGAAAACATTGGGTAGCCATTCATTTAATCCAGATGTGGAAATCCTAGAGAGTACAAATGAAGTTGAATCTAATTTTCCCTCAGCATCACATAATTTGATTGACCTAGACAATCCTTGCTCTTATTCATCAAATGAAGATGACGACTGGCTAGGAATTGATGAATTCATGGATGTGGATGCATATTCAGCTTTGCAAGCCCATTTTGATCATATGGATATTCCTCCTGATATTGAAGCACCAATACCTTGGATGACTCAGGATCAAAATGGAAACAAGACCAACATCGAAACTAGCAGAACCTCATGGTCAACATTAGCAAAACCTCAAAGCGTACCAGGTTCTGTTAATCATCTAGGAAAATCAACTACTCAAGTTCAGGTGAAGGAAGTTTCAAATGGCCTACACTTGCCAGCTGACATTAATGTTCTGAATCATCTCCATTCTTTTGGTTCGTTTCCTCCACAAGATATTCAACCAAATAaAAAACCAGCTGCATCTCGCAGGGACAAACATGAGGTTAGTCACTTAAATTCTTTACTTGGTATATATGCAGCTAAATCCAGATGGTTTTTGGATCCTTTTAAGCATAAAAAGAAGCTACATGGTTCAAATAACATTTCTATTAACTCGTCGGATGCAATGAAGCTCCATAATGGAGGTGAGACATCTGATGCATCTTACAGTGTAAAGAAGCAAGTCACTTCACATGGTATGCCCCATCCAAACTTCCCTGAATCTGTGGACTTCATTGAATATCTGGGAGCTGGACATTCCATGCCTCAGTGGCCGGAATATGCAAAAACGAAAACCAAGGAGCCATTTTTCCCCCCTCATATGGCCTCAGGTTTTtATGGGTCATTTAATCCTTTTGTTCCTCCTACCCTTGGAGAAGTTGTTGACGTGCCATGGGTTCAGGGTTCCACCCAAAATCTTCCAAATATTGCTCCAAATGACTCAATCAGCGAAGCAATTCCACCTGCAGACAAGGATAAATTCTTGGGGAATTTTGAGAACTTTAAACAATTTGATACTATTGACGATCATTCTGACCATCACTATGCTAGCAAAGGTTCTAGCTTGAACCAGGTATAAGTCTTGAAAAATTATTCTTTTGTTTATGAATACTTTTATTTATTTCAGGAGGTTTTAGACATTCTCTTGTTGCCAGGCATCAAAGAAATGGACAAAGAAAATTCAAGACGATTGGAAGATCCTGCAGAATGATTTACCTGGTAGGTTTCCCTTTTCTTTTCCTCCATTTTGAACATATTGTAGATTCATTTTAACATAAGCTCTGAAAGATTTAGCTGTAGGAAATGTCAAGCTTTTGAATAAACGGTAATTGACTTCACTTTTATCCTTTTGAATTCTATACCACAGAAACTATATTTGTTAGAGTATATGAGTCAAGAATGGATCTTATGAGGGCTGTCATCATTGGAGCACAGGGTACTCCATACCATGATGGATTATTTTtCTTTGACATATTTTtCCCTCCTCGTTATCCAGACCTACCACCAGTATGTGTATATGTTCATAATTTTTGGTGTTTATTCTATAGTTATTACTATACTGTGCTTGGATTTTCTGACAGACAATGTTCTCTTAAATGCAGCAAGTTTATTACCACTCTAAGGGCCTTCGACTCAACCCGAACTTGTATAATTGTGGGAAAGTATGTCTAAGTCTGCTTAACACCTGGCGTGGCAATGGAAATGAGAATTGGGTTCCTGGCATGTCAACTATGCTTCAAGTTCTTGTCTCTATCCAAGGATTGATCTTGAATACGAAACCCTACTTTAATGAGCCAGGGTATGCATATCAAAGTGGATCGGAGGCAGGTGAAAATAGATCTCACATGTATAATGAGGAGACTTACATCCTATCCATTAAAACCATGTTGTTTAACATCAGGAGACCACCAAAGGTATGGTTCTAGACATCTTTTCCTGTTTGGGGCCTTTTAGTAGGTTGAATACAATATCAAGAGATGGTATTATCTTAGAACTGAAGGATAAGATGTACAACCTATTAAAACAGATCTTGTTTAGTGAGGTCCAGGTGGGATTATATTAATAACTAAGATTGTAAATTTTAGCATTCGAGTAGGATGCTTAATAAGAGAATAGCCTTGTCATTACTTAGGAGGTAGATTGGGACGTGCAAATATCAAAGCCGAGTTTGTTAAATGTATTTAGTTGTAATGTTTTATCAAGTGTATGCTTATTTAAATGATGTTCATGACCCTTGTTGTTGAATAAATTAAGACTAGAATAACTTCTGCTGCAGCATTTTGAGGACTTTGTTAGGGGACATTTTTACCAAAGGGCTCATGATATTCTGGTGGCCTGCAAAGCATATATCAATGGTGCTCAGGTGGGATCGCTAGTTGAAGGCGGGGTTCAAGATCTCGAGCTGAGCGACAAGAGCTGCTCGGACAGTTTTAAGCAATCTGTGACCCATCTTTTGAGTCAGCTTGTTGCGGCTTTTAAGGGAATTGGAGTCGGTGACTGCGACAAGTACCTGCAAATGCTATCGCCAACCGTTCCAAACACAAGAAAGCGACCAAGTAGACCAGTAAAGCTCAAGTAGAATAGAAAGGGTTGGAAGGCGATGGTGTCGGGAAGTTGGTTTTTGGTATAGATTTTGAACATGTTGACGTCTAACTGAAACAAACTTGAGATTTGTTAACTGTCCAAGGTAGTTTAGAAGGTTAGCGTTTAGAGCAATCATAGTAGAGCTTGCTTGCTTCCCTATGTCTATAATTTTTCTCATAATGAACACATATGATCTCTAATTGTGTACATAGTCTCTATATGCACCAGAGGATAATGTCTTTCCATTTCAACACGGACAGTGGTATACTATGTAACTTATTTTCTTCCTCTCAAGGTAATAATGACGCTTTTT

mRNA sequence

TTTATTTTTATATActtcttctcacttctccctattcttttttacttcttatttccttctttgtctcttcctattcttcttctCTAAATCATCCGATTGTTGTTTTTTTCTCGCTATGTCATCAGGAAACGAGAACTATCATCTGGTTCCGCTTCTGGGTTTGTGAAATCCCACCACCCGATGATCAGACGCTCTTCCAAAATGCTAAAAAACAAAGGGGTTATTTCACTCGATGTGATTGATGTAGACAAAGATGAAGCTTCCGATGCCATGTTTATTCACCAGAAGTTTGATGCAAATGACAAAGGAAAGGGTATATATCACCATGGTGATCATCAATTGAAGAAAACATTGGGTAGCCATTCATTTAATCCAGATGTGGAAATCCTAGAGAGTACAAATGAAGTTGAATCTAATTTTCCCTCAGCATCACATAATTTGATTGACCTAGACAATCCTTGCTCTTATTCATCAAATGAAGATGACGACTGGCTAGGAATTGATGAATTCATGGATGTGGATGCATATTCAGCTTTGCAAGCCCATTTTGATCATATGGATATTCCTCCTGATATTGAAGCACCAATACCTTGGATGACTCAGGATCAAAATGGAAACAAGACCAACATCGAAACTAGCAGAACCTCATGGTCAACATTAGCAAAACCTCAAAGCGTACCAGGTTCTGTTAATCATCTAGGAAAATCAACTACTCAAGTTCAGGTGAAGGAAGTTTCAAATGGCCTACACTTGCCAGCTGACATTAATGTTCTGAATCATCTCCATTCTTTTGGTTCGTTTCCTCCACAAGATATTCAACCAAATAAAAAACCAGCTGCATCTCGCAGGGACAAACATGAGCTCCATAATGGAGGTGAGACATCTGATGCATCTTACAGTGTAAAGAAGCAAGTCACTTCACATGGTATGCCCCATCCAAACTTCCCTGAATCTGTGGACTTCATTGAATATCTGGGAGCTGGACATTCCATGCCTCAGTGGCCGGAATATGCAAAAACGAAAACCAAGGAGCCATTTTTCCCCCCTCATATGGCCTCAGGTTTTTATGGGTCATTTAATCCTTTTGTTCCTCCTACCCTTGGAGAAGTTGTTGACGTGCCATGGGTTCAGGGTTCCACCCAAAATCTTCCAAATATTGCTCCAAATGACTCAATCAGCGAAGCAATTCCACCTGCAGACAAGGATAAATTCTTGGGGAATTTTGAGAACTTTAAACAATTTGATACTATTGACGATCATTCTGACCATCACTATGCTAGCAAAGGTTCTAGCTTGAACCAGGCATCAAAGAAATGGACAAAGAAAATTCAAGACGATTGGAAGATCCTGCAGAATGATTTACCTGAAACTATATTTGTTAGAGTATATGAGTCAAGAATGGATCTTATGAGGGCTGTCATCATTGGAGCACAGGGTACTCCATACCATGATGGATTATTTTTCTTTGACATATTTTTCCCTCCTCGTTATCCAGACCTACCACCACAAGTTTATTACCACTCTAAGGGCCTTCGACTCAACCCGAACTTGTATAATTGTGGGAAAGTATGTCTAAGTCTGCTTAACACCTGGCGTGGCAATGGAAATGAGAATTGGGTTCCTGGCATGTCAACTATGCTTCAAGTTCTTGTCTCTATCCAAGGATTGATCTTGAATACGAAACCCTACTTTAATGAGCCAGGGTATGCATATCAAAGTGGATCGGAGGCAGGTGAAAATAGATCTCACATGTATAATGAGGAGACTTACATCCTATCCATTAAAACCATGTTGTTTAACATCAGGAGACCACCAAAGCATTTTGAGGACTTTGTTAGGGGACATTTTTACCAAAGGGCTCATGATATTCTGGTGGCCTGCAAAGCATATATCAATGGTGCTCAGGTGGGATCGCTAGTTGAAGGCGGGGTTCAAGATCTCGAGCTGAGCGACAAGAGCTGCTCGGACAGTTTTAAGCAATCTGTGACCCATCTTTTGAGTCAGCTTGTTGCGGCTTTTAAGGGAATTGGAGTCGGTGACTGCGACAAGTACCTGCAAATGCTATCGCCAACCGTTCCAAACACAAGAAAGCGACCAAGTAGACCAGTAAAGCTCAAGTAGAATAGAAAGGGTTGGAAGGCGATGGTGTCGGGAAGTTGGTTTTTGGTATAGATTTTGAACATGTTGACGTCTAACTGAAACAAACTTGAGATTTGTTAACTGTCCAAGGTAGTTTAGAAGGTTAGCGTTTAGAGCAATCATAGTAGAGCTTGCTTGCTTCCCTATGTCTATAATTTTTCTCATAATGAACACATATGATCTCTAATTGTGTACATAGTCTCTATATGCACCAGAGGATAATGTCTTTCCATTTCAACACGGACAGTGGTATACTATGTAACTTATTTTCTTCCTCTCAAGGTAATAATGACGCTTTTT

Coding sequence (CDS)

ATGATCAGACGCTCTTCCAAAATGCTAAAAAACAAAGGGGTTATTTCACTCGATGTGATTGATGTAGACAAAGATGAAGCTTCCGATGCCATGTTTATTCACCAGAAGTTTGATGCAAATGACAAAGGAAAGGGTATATATCACCATGGTGATCATCAATTGAAGAAAACATTGGGTAGCCATTCATTTAATCCAGATGTGGAAATCCTAGAGAGTACAAATGAAGTTGAATCTAATTTTCCCTCAGCATCACATAATTTGATTGACCTAGACAATCCTTGCTCTTATTCATCAAATGAAGATGACGACTGGCTAGGAATTGATGAATTCATGGATGTGGATGCATATTCAGCTTTGCAAGCCCATTTTGATCATATGGATATTCCTCCTGATATTGAAGCACCAATACCTTGGATGACTCAGGATCAAAATGGAAACAAGACCAACATCGAAACTAGCAGAACCTCATGGTCAACATTAGCAAAACCTCAAAGCGTACCAGGTTCTGTTAATCATCTAGGAAAATCAACTACTCAAGTTCAGGTGAAGGAAGTTTCAAATGGCCTACACTTGCCAGCTGACATTAATGTTCTGAATCATCTCCATTCTTTTGGTTCGTTTCCTCCACAAGATATTCAACCAAATAaAAAACCAGCTGCATCTCGCAGGGACAAACATGAGCTCCATAATGGAGGTGAGACATCTGATGCATCTTACAGTGTAAAGAAGCAAGTCACTTCACATGGTATGCCCCATCCAAACTTCCCTGAATCTGTGGACTTCATTGAATATCTGGGAGCTGGACATTCCATGCCTCAGTGGCCGGAATATGCAAAAACGAAAACCAAGGAGCCATTTTTCCCCCCTCATATGGCCTCAGGTTTTtATGGGTCATTTAATCCTTTTGTTCCTCCTACCCTTGGAGAAGTTGTTGACGTGCCATGGGTTCAGGGTTCCACCCAAAATCTTCCAAATATTGCTCCAAATGACTCAATCAGCGAAGCAATTCCACCTGCAGACAAGGATAAATTCTTGGGGAATTTTGAGAACTTTAAACAATTTGATACTATTGACGATCATTCTGACCATCACTATGCTAGCAAAGGTTCTAGCTTGAACCAGGCATCAAAGAAATGGACAAAGAAAATTCAAGACGATTGGAAGATCCTGCAGAATGATTTACCTGAAACTATATTTGTTAGAGTATATGAGTCAAGAATGGATCTTATGAGGGCTGTCATCATTGGAGCACAGGGTACTCCATACCATGATGGATTATTTTtCTTTGACATATTTTtCCCTCCTCGTTATCCAGACCTACCACCACAAGTTTATTACCACTCTAAGGGCCTTCGACTCAACCCGAACTTGTATAATTGTGGGAAAGTATGTCTAAGTCTGCTTAACACCTGGCGTGGCAATGGAAATGAGAATTGGGTTCCTGGCATGTCAACTATGCTTCAAGTTCTTGTCTCTATCCAAGGATTGATCTTGAATACGAAACCCTACTTTAATGAGCCAGGGTATGCATATCAAAGTGGATCGGAGGCAGGTGAAAATAGATCTCACATGTATAATGAGGAGACTTACATCCTATCCATTAAAACCATGTTGTTTAACATCAGGAGACCACCAAAGCATTTTGAGGACTTTGTTAGGGGACATTTTTACCAAAGGGCTCATGATATTCTGGTGGCCTGCAAAGCATATATCAATGGTGCTCAGGTGGGATCGCTAGTTGAAGGCGGGGTTCAAGATCTCGAGCTGAGCGACAAGAGCTGCTCGGACAGTTTTAAGCAATCTGTGACCCATCTTTTGAGTCAGCTTGTTGCGGCTTTTAAGGGAATTGGAGTCGGTGACTGCGACAAGTACCTGCAAATGCTATCGCCAACCGTTCCAAACACAAGAAAGCGACCAAGTAGACCAGTAAAGCTCAAGTAG

Protein sequence

MIRRSSKMLKNKGVISLDVIDVDKDEASDAMFIHQKFDANDKGKGIYHHGDHQLKKTLGSHSFNPDVEILESTNEVESNFPSASHNLIDLDNPCSYSSNEDDDWLGIDEFMDVDAYSALQAHFDHMDIPPDIEAPIPWMTQDQNGNKTNIETSRTSWSTLAKPQSVPGSVNHLGKSTTQVQVKEVSNGLHLPADINVLNHLHSFGSFPPQDIQPNKKPAASRRDKHELHNGGETSDASYSVKKQVTSHGMPHPNFPESVDFIEYLGAGHSMPQWPEYAKTKTKEPFFPPHMASGFYGSFNPFVPPTLGEVVDVPWVQGSTQNLPNIAPNDSISEAIPPADKDKFLGNFENFKQFDTIDDHSDHHYASKGSSLNQASKKWTKKIQDDWKILQNDLPETIFVRVYESRMDLMRAVIIGAQGTPYHDGLFFFDIFFPPRYPDLPPQVYYHSKGLRLNPNLYNCGKVCLSLLNTWRGNGNENWVPGMSTMLQVLVSIQGLILNTKPYFNEPGYAYQSGSEAGENRSHMYNEETYILSIKTMLFNIRRPPKHFEDFVRGHFYQRAHDILVACKAYINGAQVGSLVEGGVQDLELSDKSCSDSFKQSVTHLLSQLVAAFKGIGVGDCDKYLQMLSPTVPNTRKRPSRPVKLK*
BLAST of Cucsa.123170 vs. Swiss-Prot
Match: UBC26_ARATH (Probable ubiquitin-conjugating enzyme E2 26 OS=Arabidopsis thaliana GN=UBC26 PE=2 SV=2)

HSP 1 Score: 370.5 bits (950), Expect = 3.6e-101
Identity = 172/278 (61.87%), Postives = 217/278 (78.06%), Query Frame = 1

Query: 341 KDKFLGNFENFKQFDTIDDHSDHHYASKGSSLNQASKKWTKKIQDDWKILQNDLPETIFV 400
           K++FL    +FK+FDT++D SDHHYASKG S  Q SK W KK+Q DWKIL+NDLPE I V
Sbjct: 237 KEEFL---RDFKRFDTVEDFSDHHYASKGKSSKQHSKNWVKKVQADWKILENDLPEAISV 296

Query: 401 RVYESRMDLMRAVIIGAQGTPYHDGLFFFDIFFPPRYPDLPPQVYYHSKGLRLNPNLYNC 460
           R  ESRMDL+RAVIIGA+GTPYHDGLFFFDI FP  YP +PP V+YHS GLR+NPNLYNC
Sbjct: 297 RACESRMDLLRAVIIGAEGTPYHDGLFFFDIQFPDTYPSVPPNVHYHSGGLRINPNLYNC 356

Query: 461 GKVCLSLLNTWRGNGNENWVPGMSTMLQVLVSIQGLILNTKPYFNEPGYAYQSGSEAGEN 520
           GKVCLSLL TW G+  E W+P  STMLQ+LVSIQ LILN KPYFNEPGY   +G+ +GE+
Sbjct: 357 GKVCLSLLGTWAGSAREKWLPNESTMLQLLVSIQALILNEKPYFNEPGYVQSAGTASGES 416

Query: 521 RSHMYNEETYILSIKTMLFNIRRPPKHFEDFVRGHFYQRAHDILVACKAYINGAQVGSLV 580
           +S +Y+E  ++LS+KTM+++IRRPP+HFE++V+ H++ R+HDI+ AC AY  GA +GS+V
Sbjct: 417 KSKVYSENVFLLSLKTMVYSIRRPPQHFEEYVQNHYFVRSHDIVKACNAYKAGAPLGSMV 476

Query: 581 EGGVQDLELSDKSCSDSFKQSVTHLLSQLVAAFKGIGV 619
           +GGVQDLE + +S S  FK  V   +  +V  F  +GV
Sbjct: 477 KGGVQDLEEARQSGSKKFKTDVASFMQTVVDEFVKLGV 511

BLAST of Cucsa.123170 vs. Swiss-Prot
Match: UBC25_ARATH (Probable ubiquitin-conjugating enzyme E2 25 OS=Arabidopsis thaliana GN=UBC25 PE=2 SV=1)

HSP 1 Score: 368.6 bits (945), Expect = 1.4e-100
Identity = 214/464 (46.12%), Postives = 275/464 (59.27%), Query Frame = 1

Query: 183 KEVSNGLHLPADINVLNHLHSFGSFPPQDIQPNKKPAASRRDKHELHNGGETSDASYSVK 242
           +++ +G  +P  + V     S   FP      +K+ A+S +  H  +NG  +S      K
Sbjct: 128 QDLFDGKDIPTGVEV-----SMDWFPNS---ADKESASSSKSSHA-NNGNNSS------K 187

Query: 243 KQVTSHGMPHPNFPESVDFIEYLGAGHSMPQWPE-------YAKTKTKEPFFPPH----- 302
           K   + G+ H  F   ++        +++P   E       YA  +  + F PP+     
Sbjct: 188 KATKASGI-HSQFSSDME-TPVAQPWNALPHKAEGVIPNSAYALPQNSKAFQPPYAVHYS 247

Query: 303 -MASGFYGSFNPFVPPTLGEVVDVPWVQGSTQNLPNIAPNDSISEA--------IPPADK 362
            + + F     P  P T+      P   GS+  LP   P    + A        +PP D 
Sbjct: 248 ALKTAFSNYLQPQTPDTVLGEAPAP-AAGSSGLLPPNTPGFKSNAARFKEEPPILPPDDS 307

Query: 363 ------DKFLGNFENFKQFDTIDDHSDHHYASKGSSLNQASKKWTKKIQDDWKILQNDLP 422
                 + +LG +  FK+FD ++D SDHHYASKG++  Q SK W K+IQD+W+IL+ DLP
Sbjct: 308 RVKRNMEDYLGLYLFFKRFDIVEDFSDHHYASKGTTSKQHSKDWAKRIQDEWRILEKDLP 367

Query: 423 ETIFVRVYESRMDLMRAVIIGAQGTPYHDGLFFFDIFFPPRYPDLPPQVYYHSKGLRLNP 482
           E IFVR YESRMDL+RAVIIGAQGTPYHDGLFFFDIFFP  YP  PP V+YHS GLR+NP
Sbjct: 368 EMIFVRAYESRMDLLRAVIIGAQGTPYHDGLFFFDIFFPDTYPSTPPIVHYHSGGLRINP 427

Query: 483 NLYNCGKVCLSLLNTWRGNGNENWVPGMSTMLQVLVSIQGLILNTKPYFNEPGYAYQSGS 542
           NLYNCGKVCLSLL TW GN  E W+P  STMLQVLVSIQGLILN KPYFNEPGY   +GS
Sbjct: 428 NLYNCGKVCLSLLGTWSGNQREKWIPNTSTMLQVLVSIQGLILNQKPYFNEPGYERSAGS 487

Query: 543 EAGENRSHMYNEETYILSIKTMLFNIRRPPKHFEDFVRGHFYQRAHDILVACKAYINGAQ 602
             GE+ S  Y+E T+ILS+KTM++ +RRPPK+FEDF  GHF+  AHD+L AC AY NGA 
Sbjct: 488 AHGESTSKAYSENTFILSLKTMVYTMRRPPKYFEDFAYGHFFSCAHDVLKACNAYRNGAT 547

Query: 603 VGSLVEGGVQDLELSDKSCSDSFKQSV-THLLSQLVAAFKGIGV 619
            G LV+G     E S    S  F+  V T + + L+  F  +GV
Sbjct: 548 PGYLVKGAPDVEENSAGMSSLKFRTDVATFVETVLLKEFILLGV 573

BLAST of Cucsa.123170 vs. Swiss-Prot
Match: UBC38_ARATH (Putative ubiquitin-conjugating enzyme E2 38 OS=Arabidopsis thaliana GN=UBC38 PE=3 SV=2)

HSP 1 Score: 366.7 bits (940), Expect = 5.2e-100
Identity = 168/278 (60.43%), Postives = 216/278 (77.70%), Query Frame = 1

Query: 341 KDKFLGNFENFKQFDTIDDHSDHHYASKGSSLNQASKKWTKKIQDDWKILQNDLPETIFV 400
           K++FL    NFK+FD+++D SDHHY SKG +  Q SK W KK+QD+WKIL  +LPETIFV
Sbjct: 20  KEEFL---RNFKRFDSVEDFSDHHYVSKGKASKQHSKNWVKKVQDEWKILNQNLPETIFV 79

Query: 401 RVYESRMDLMRAVIIGAQGTPYHDGLFFFDIFFPPRYPDLPPQVYYHSKGLRLNPNLYNC 460
           R  ESRMDL+RAVIIGA+GTPYHDGLFFFDI FP  YP +PP VYYHS GLR+NPNLYNC
Sbjct: 80  RACESRMDLLRAVIIGAEGTPYHDGLFFFDIQFPDTYPSVPPNVYYHSGGLRINPNLYNC 139

Query: 461 GKVCLSLLNTWRGNGNENWVPGMSTMLQVLVSIQGLILNTKPYFNEPGYAYQSGSEAGEN 520
           GKVCLSLL TW GN  ++W+P  STMLQVLVSIQ L+LN +PYFNEPGY    G+  GE+
Sbjct: 140 GKVCLSLLGTWHGNARQSWLPKESTMLQVLVSIQALVLNEQPYFNEPGYGLIKGTWLGES 199

Query: 521 RSHMYNEETYILSIKTMLFNIRRPPKHFEDFVRGHFYQRAHDILVACKAYINGAQVGSLV 580
           +S +Y+E  ++LS+KTM++++R+PP+HFE++V+ H++ R+HDI+ AC AY  GA +GS+V
Sbjct: 200 KSKVYSENVFLLSLKTMVYSMRKPPQHFEEYVQNHYFVRSHDIVKACNAYKAGAPLGSMV 259

Query: 581 EGGVQDLELSDKSCSDSFKQSVTHLLSQLVAAFKGIGV 619
           +GGVQDLE + +S S  FK  V   +  +V  F  +GV
Sbjct: 260 KGGVQDLEQARQSGSKKFKTDVASFMQTVVDEFVKLGV 294

BLAST of Cucsa.123170 vs. Swiss-Prot
Match: UBC39_ARATH (Putative ubiquitin-conjugating enzyme E2 39 OS=Arabidopsis thaliana GN=UBC39 PE=3 SV=1)

HSP 1 Score: 307.8 bits (787), Expect = 2.9e-82
Identity = 154/286 (53.85%), Postives = 199/286 (69.58%), Query Frame = 1

Query: 341 KDKFLGNFENFKQFDTIDDHSDHHYASKGSSLNQA---------SKKWTKKIQDDWKILQ 400
           K++FL    +FK+FDT++D SDHHYA++GS    +          K W K IQ +WKIL 
Sbjct: 14  KEEFL---RDFKRFDTVEDFSDHHYAAEGSPAKLSLWHLKFKGRPKNWVKDIQKEWKILD 73

Query: 401 NDLPETIFVRVYESRMDLMRAVIIGAQGTPYHDGLFFFDIFFPPRYPDLPPQVYYHSKGL 460
            +LPETIFVR  ESR+DL+RAVIIGA+GTPYHDGLFFFDI FP  YP +PP+V+YHS GL
Sbjct: 74  KNLPETIFVRACESRIDLLRAVIIGAEGTPYHDGLFFFDIQFPDTYPSVPPKVHYHSGGL 133

Query: 461 RLNPNLYNCGKVCLSLLNTWRGNGNENWVPGMSTMLQVLVSIQGLILNTKPYFNEPGYAY 520
           R+NPNLY CGKVCLSL++TW G   E W+P  STMLQ+LVSIQ LILN KPY+NEPGY  
Sbjct: 134 RINPNLYKCGKVCLSLISTWTGKKREKWLPKESTMLQLLVSIQALILNEKPYYNEPGYEK 193

Query: 521 QSGSEAGENRSHMYNEETYILSIKTMLFNIRRPPKHFEDFVRGHFYQRAHDILVACKAYI 580
             G+  GE+ S  Y+E  ++ S+KTM         HFE+FVR HF+ R+HDI+ AC AY 
Sbjct: 194 SMGTPLGESYSKDYSENVFVFSLKTM---------HFEEFVRSHFFVRSHDIVKACNAYK 253

Query: 581 NGAQVGSLVEGGVQDLELSDKSCSDSFKQSVTHLLSQLVAAFKGIG 618
           +GA VGS+ +GGV+  + + +  S  F+ +VT  +  +V  F  +G
Sbjct: 254 DGAPVGSIDKGGVK--KQTRQRGSLKFRINVTSFMKTVVDEFVNLG 285

BLAST of Cucsa.123170 vs. Swiss-Prot
Match: UBC24_ARATH (Probable ubiquitin-conjugating enzyme E2 24 OS=Arabidopsis thaliana GN=UBC24 PE=1 SV=1)

HSP 1 Score: 297.7 bits (761), Expect = 3.0e-79
Identity = 145/277 (52.35%), Postives = 189/277 (68.23%), Query Frame = 1

Query: 347 NFENFKQFDTIDDHSDHHYAS--KGSSLNQASKKWTKKIQDDWKILQNDLPETIFVRVYE 406
           N   F+QFD ++D SDHH+ S  KG + +Q +K W KK+Q +W  L+ +LP TI+VRV E
Sbjct: 629 NQVRFRQFDMVNDCSDHHFLSSDKGLAQSQVTKSWVKKVQQEWSNLEANLPNTIYVRVCE 688

Query: 407 SRMDLMRAVIIGAQGTPYHDGLFFFDIFFPPRYPDLPPQVYYHSKGLRLNPNLYNCGKVC 466
            RMDL+RA ++GA GTPYHDGLFFFDI  PP+YP  PP V+YHS G+RLNPNLY  G+VC
Sbjct: 689 ERMDLLRAALVGAPGTPYHDGLFFFDIMLPPQYPHEPPMVHYHSGGMRLNPNLYESGRVC 748

Query: 467 LSLLNTWRGNGNENWVPGMSTMLQVLVSIQGLILNTKPYFNEPGYAYQSGSEAGENRSHM 526
           LSLLNTW G+G E W  G S++LQ+L+S Q L+LN KPYFNE GY  Q G   GE  S  
Sbjct: 749 LSLLNTWSGSGTEVWNAGSSSILQLLLSFQALVLNEKPYFNEAGYDKQLGRAEGEKNSVS 808

Query: 527 YNEETYILSIKTMLFNIRRPPKHFEDFVRGHFYQRAHDILVACKAYINGAQVGSLVEGGV 586
           YNE  ++++ K+M+  +R+PPKHFE  V+ HF  RA  +L ACKAY+ G  VGS      
Sbjct: 809 YNENAFLITCKSMISMLRKPPKHFEMLVKDHFTHRAQHVLAACKAYMEGVPVGSSA---- 868

Query: 587 QDLELSDKSCSDSFKQSVTHLLSQLVAAFKGIGVGDC 622
            +L+ +  + S  FK  ++ L  +L+ AF  IGV DC
Sbjct: 869 -NLQGNSTTNSTGFKIMLSKLYPKLLEAFSEIGV-DC 899

BLAST of Cucsa.123170 vs. TrEMBL
Match: A0A0A0KZM7_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_4G637700 PE=4 SV=1)

HSP 1 Score: 1344.3 bits (3478), Expect = 0.0e+00
Identity = 645/646 (99.85%), Postives = 645/646 (99.85%), Query Frame = 1

Query: 1   MIRRSSKMLKNKGVISLDVIDVDKDEASDAMFIHQKFDANDKGKGIYHHGDHQLKKTLGS 60
           MIRRSSKMLKNKGVISLDVIDVDKDEASDAMFIHQKFDANDKGKGIYHHGDHQLKKTLGS
Sbjct: 1   MIRRSSKMLKNKGVISLDVIDVDKDEASDAMFIHQKFDANDKGKGIYHHGDHQLKKTLGS 60

Query: 61  HSFNPDVEILESTNEVESNFPSASHNLIDLDNPCSYSSNEDDDWLGIDEFMDVDAYSALQ 120
           HSFNPDVEILESTNEVESNFPSASHNLIDLDNPCSYSSNEDDDWLGIDEFMDVDAYSALQ
Sbjct: 61  HSFNPDVEILESTNEVESNFPSASHNLIDLDNPCSYSSNEDDDWLGIDEFMDVDAYSALQ 120

Query: 121 AHFDHMDIPPDIEAPIPWMTQDQNGNKTNIETSRTSWSTLAKPQSVPGSVNHLGKSTTQV 180
           AHFDHMDIPPDIEAPIPWMTQDQNGNKTNIETSRTSWSTLAKPQSVPGSVNHLGKSTTQV
Sbjct: 121 AHFDHMDIPPDIEAPIPWMTQDQNGNKTNIETSRTSWSTLAKPQSVPGSVNHLGKSTTQV 180

Query: 181 QVKEVSNGLHLPADINVLNHLHSFGSFPPQDIQPNKKPAASRRDKHELHNGGETSDASYS 240
           QVKEVSNGLHLPADINVLNHLHSFGSFPPQDIQPNKKPAASRRDKHELHNGGETSDASYS
Sbjct: 181 QVKEVSNGLHLPADINVLNHLHSFGSFPPQDIQPNKKPAASRRDKHELHNGGETSDASYS 240

Query: 241 VKKQVTSHGMPHPNFPESVDFIEYLGAGHSMPQWPEYAKTKTKEPFFPPHMASGFYGSFN 300
           VKKQVTSHGMPHPNFPESVDFIEYLGAGHSMPQWPEYAKTKTKEPFFPPHMASGFYGSFN
Sbjct: 241 VKKQVTSHGMPHPNFPESVDFIEYLGAGHSMPQWPEYAKTKTKEPFFPPHMASGFYGSFN 300

Query: 301 PFVPPTLGEVVDVPWVQGSTQNLPNIAPNDSISEAIPPADKDKFLGNFENFKQFDTIDDH 360
           PFVPPTLGEVVDVPWVQGSTQNLPNIAPNDSISEAIPPADKDKFLGNFENFKQFDTIDDH
Sbjct: 301 PFVPPTLGEVVDVPWVQGSTQNLPNIAPNDSISEAIPPADKDKFLGNFENFKQFDTIDDH 360

Query: 361 SDHHYASKGSSLNQASKKWTKKIQDDWKILQNDLPETIFVRVYESRMDLMRAVIIGAQGT 420
           SDHHYASKGSSLNQASKKWTKKIQDDWKILQNDLPETIFVRVYESRMDLMRAVIIGAQGT
Sbjct: 361 SDHHYASKGSSLNQASKKWTKKIQDDWKILQNDLPETIFVRVYESRMDLMRAVIIGAQGT 420

Query: 421 PYHDGLFFFDIFFPPRYPDLPPQVYYHSKGLRLNPNLYNCGKVCLSLLNTWRGNGNENWV 480
           PYHDGLFFFDIFFPPRYPDLPPQVYYHSKGLRLNPNLYNCGKVCLSLLNTWRGNGNENWV
Sbjct: 421 PYHDGLFFFDIFFPPRYPDLPPQVYYHSKGLRLNPNLYNCGKVCLSLLNTWRGNGNENWV 480

Query: 481 PGMSTMLQVLVSIQGLILNTKPYFNEPGYAYQSGSEAGENRSHMYNEETYILSIKTMLFN 540
           PGMSTMLQVLVSIQGLILNTKPYFNEPGYAYQSGSEAGENRSHMYNEETYILSIKTMLFN
Sbjct: 481 PGMSTMLQVLVSIQGLILNTKPYFNEPGYAYQSGSEAGENRSHMYNEETYILSIKTMLFN 540

Query: 541 IRRPPKHFEDFVRGHFYQRAHDILVACKAYINGAQVGSLVEGGVQDLELSDKSCSDSFKQ 600
           IRRPPKHFEDFVRGHFYQRAHDILVACKAYINGAQVGSLVEGGVQDLELSDKSCSDSFKQ
Sbjct: 541 IRRPPKHFEDFVRGHFYQRAHDILVACKAYINGAQVGSLVEGGVQDLELSDKSCSDSFKQ 600

Query: 601 SVTHLLSQLVAAFKGIGVGDCDKYLQMLSPTVPNTRKRPSRPVKLK 647
           SVTHLLSQLVAAFKGIGV DCDKYLQMLSPTVPNTRKRPSRPVKLK
Sbjct: 601 SVTHLLSQLVAAFKGIGVSDCDKYLQMLSPTVPNTRKRPSRPVKLK 646

BLAST of Cucsa.123170 vs. TrEMBL
Match: A0A061F7Z1_THECC (Ubiquitin-conjugating enzyme 25, putative isoform 1 OS=Theobroma cacao GN=TCM_031512 PE=4 SV=1)

HSP 1 Score: 510.8 bits (1314), Expect = 2.5e-141
Identity = 309/686 (45.04%), Postives = 408/686 (59.48%), Query Frame = 1

Query: 2   IRRSSKMLKNKGVISLDVIDVDKDEAS-DAMFIHQKFDANDKGKGIYHHGDHQLKKTLGS 61
           I RS K  K K VI  +VIDVDK+E S D M + ++ DA +KGK +        K + G 
Sbjct: 12  IPRSFKS-KQKQVIIHEVIDVDKEEDSADIMILDERVDARNKGKAV--------KSSSGG 71

Query: 62  HSFNPDVEILE----STNEVE-SNFPS-ASHNLIDLDNPCSYSSNEDDDWLGIDEFMDVD 121
           +S     + ++    S N+VE S  P+  S N+++LD   SY     D++  +++ MDVD
Sbjct: 72  YSTIRAEDFVDKSLGSINKVEPSKHPTQGSQNIVNLDCDLSYDDEIFDNYY-LNDIMDVD 131

Query: 122 AYSALQAHFDHMDIPPDIEAPIPWMT-------QDQNGN-KTNIETSRTSWST-LAKPQS 181
            Y+ LQAHFD++DIP  +EA IPW         +  +GN  + ++ S  S S  L++P  
Sbjct: 132 DYAMLQAHFDNVDIPAGVEASIPWFADFSESKKKTSHGNISSGVDGSDQSLSPWLSEPTH 191

Query: 182 VPGSVNHLGKSTTQVQVKEVSNGLHLPADINVLNHLHSFGSFPPQDIQPNKKPAASR--- 241
                  +  S+ Q     +S   H P + ++ + L       PQ  Q  KK A S+   
Sbjct: 192 TNKKAALVNSSSFQTPGDPLS---HSPGEASLSSPL-----LFPQGSQSKKKSATSQPRW 251

Query: 242 -----RDKHELHN---GGETSDASYS-----------------------VKKQVTSHGMP 301
                +  H L +   GG T+ ++ S                       ++KQ+ +  + 
Sbjct: 252 SSQNLQFGHSLQSSQAGGSTNGSNVSHSDAMILPHAVTPAAYWDHINSAIQKQIGASSLF 311

Query: 302 HPNFPESVDFI-EYLGAGHSMPQWPEYAKTKTKEPFFPPHMASGFYGSFNPFVPPTLGEV 361
           H NFP  +D +   L    S+  WP     K+K  F   +M S F             + 
Sbjct: 312 HSNFPTPLDALNNTLAVEPSVSWWP---PMKSKFSFNKHNMYSSF------------PDP 371

Query: 362 VDVPWVQGSTQNLPNIAPNDSISEAIPPADKDKFLGNFENFKQFDTIDDHSDHHYASKGS 421
           VD  ++  + Q +  I     ++E       ++ L   + FKQFDT+DD SDHHYAS G+
Sbjct: 372 VDGAYI--TPQEVAEIRNQRIVNE-------EEILSKLQLFKQFDTVDDFSDHHYASSGA 431

Query: 422 SLNQASKKWTKKIQDDWKILQNDLPETIFVRVYESRMDLMRAVIIGAQGTPYHDGLFFFD 481
           S  Q  K W KKIQ++WKIL+ DLP+TIFVRVYESRMDL+RAVIIGA+GTPYHDGLFFFD
Sbjct: 432 STKQPPKNWAKKIQEEWKILEKDLPDTIFVRVYESRMDLLRAVIIGAEGTPYHDGLFFFD 491

Query: 482 IFFPPRYPDLPPQVYYHSKGLRLNPNLYNCGKVCLSLLNTWRGNGNENWVPGMSTMLQVL 541
           +FFP  YP +PP VYYHS GLRLNPNLY+CGKVCLSLLNTW GN NE W+PGMSTMLQVL
Sbjct: 492 VFFPASYPKVPPHVYYHSGGLRLNPNLYSCGKVCLSLLNTWSGNKNEKWIPGMSTMLQVL 551

Query: 542 VSIQGLILNTKPYFNEPGYAYQSGSEAGENRSHMYNEETYILSIKTMLFNIRRPPKHFED 601
           VSIQ LILN KPYFNEPG+A+  G+  GE+ S  YNEET+ILS+KTM +++RRPPKHFED
Sbjct: 552 VSIQALILNQKPYFNEPGWAHHCGTPKGESLSRQYNEETFILSLKTMTYSMRRPPKHFED 611

Query: 602 FVRGHFYQRAHDILVACKAYINGAQVGSLVEGGVQDLELSDKSCSDSFKQSVTHLLSQLV 637
           FV GHFY+RAHDILVACKAY++GAQVG LV+GGVQD++  DKSCS  FK SV   ++ LV
Sbjct: 612 FVVGHFYKRAHDILVACKAYMDGAQVGCLVKGGVQDVDEGDKSCSQKFKDSVAGCVNMLV 653

BLAST of Cucsa.123170 vs. TrEMBL
Match: I1LV04_SOYBN (Uncharacterized protein OS=Glycine max GN=GLYMA_12G224200 PE=4 SV=2)

HSP 1 Score: 496.5 bits (1277), Expect = 4.9e-137
Identity = 299/697 (42.90%), Postives = 400/697 (57.39%), Query Frame = 1

Query: 2   IRRSSKMLKNKGVISLDVIDVDKDEASDAMFIHQKFDANDKGKGIYH-HGDHQLKKTLGS 61
           I    +  +   VI  DVID+D D++ D + I +K + ++KGK I   H DHQ+ KT   
Sbjct: 12  IHHPPRFREQNKVILHDVIDID-DDSEDVVIIGEKVNNSNKGKTIDAIHDDHQVVKTADY 71

Query: 62  HSFNPDVEILESTNEVES--NFPSASHNLIDLDNPCSYSSNEDDDWLGI--DEFMDVDAY 121
               P VE   S + + S  NFPS S+NLI++D   S  S +DDD+  I  +++MDVD Y
Sbjct: 72  TYSLPAVENFGSVSGIASSNNFPSVSNNLINIDGHGSDLSYDDDDYFDILSEDYMDVDEY 131

Query: 122 SALQAHFDHMDIPPDIEAPIPWMTQD--------------------QNGNK--------- 181
           + LQ HFD++DIP  IEAP  W+  D                     N NK         
Sbjct: 132 ALLQKHFDNVDIPTGIEAPFTWLPPDYVVGSKKTGNNTLYPWHHMQSNANKSPMTPSSQP 191

Query: 182 ------TNIETSRTSWSTLAKPQSVPGSVNHL-GKSTTQVQVKEVS----------NGLH 241
                 TN E   +SW  +  P  + G+V+H  G   +   + E +           G  
Sbjct: 192 SLSLEPTNSEIQASSWGAINIPIKM-GNVDHSSGVELSSQYISEPAPSKKSATSKLRGRT 251

Query: 242 LPADINVLNHLHSFGSFPPQDIQPNKKPAAS------RRDKHELHNGGETS--DASYSVK 301
             A + V +    + S P       KKPA++        +  ++ + GE        S K
Sbjct: 252 SNASLGVESSKSHWSSGP---FHSKKKPASTYHGFNYHAEAAKMPHAGELPYWGQFKSAK 311

Query: 302 KQVTSHGMPHPNFPESVDFIEYLGAGH-----SMPQWPEYAKTKTKEPFFPPHMASGFYG 361
           K+  S      +      FIE+ G+ +     S   W E +           ++ +  Y 
Sbjct: 312 KEAGS------SISSLSTFIEHHGSLYPPGIESGKHWWEISHNAKPFSTHLNNIPNHIYY 371

Query: 362 SFNPFVPPTLGEVVDVPWVQGSTQNLPNIAPNDSISEAIPPADKDKFLGNFENFKQFDTI 421
            F+P V      V D  WV  S ++  N    D     I    K++ L  F +FKQFDT+
Sbjct: 372 PFDPLVAHP-EHVFDNNWVHDSARDGFNGKTVDGPIVTITDEAKEEILRKFRSFKQFDTV 431

Query: 422 DDHSDHHYASKGSSLNQASKKWTKKIQDDWKILQNDLPETIFVRVYESRMDLMRAVIIGA 481
           +D SDHH+    SS++Q  K W KKIQ++W+IL+ DLP +IFVRV+ESRMDL+RAVIIGA
Sbjct: 432 EDISDHHFFRSNSSMHQPPKNWAKKIQEEWRILEKDLPASIFVRVFESRMDLLRAVIIGA 491

Query: 482 QGTPYHDGLFFFDIFFPPRYPDLPPQVYYHSKGLRLNPNLYNCGKVCLSLLNTWRGNGNE 541
           +GTPYHDGLFFFD+FFP  YP++PP+V+YHS GLRLNPNLY CGKVCLSLLNTW G+ NE
Sbjct: 492 EGTPYHDGLFFFDVFFPSAYPNVPPKVHYHSGGLRLNPNLYACGKVCLSLLNTWSGSKNE 551

Query: 542 NWVPGMSTMLQVLVSIQGLILNTKPYFNEPGYAYQSGSEAGENRSHMYNEETYILSIKTM 601
            W+PG+ST+LQVLVSIQGLILNTKPYFNEPGYA+ SGS  GE  S  YNE+T+ILS++TM
Sbjct: 552 KWLPGVSTILQVLVSIQGLILNTKPYFNEPGYAHMSGSANGEKMSFQYNEDTFILSLRTM 611

Query: 602 LFNIRRPPKHFEDFVRGHFYQRAHDILVACKAYINGAQVGSLVEGGVQDLELSDKSCSDS 635
           ++ IR+PPK+FEDFV+GHF  RA DILVACKAY+ GAQVG LV+GGVQD++  D+SCS  
Sbjct: 612 MYMIRKPPKNFEDFVKGHFCSRASDILVACKAYMEGAQVGCLVKGGVQDVDEGDRSCSQR 671

BLAST of Cucsa.123170 vs. TrEMBL
Match: A0A0B2R8C8_GLYSO (Putative ubiquitin-conjugating enzyme E2 26 OS=Glycine soja GN=glysoja_007789 PE=4 SV=1)

HSP 1 Score: 496.1 bits (1276), Expect = 6.4e-137
Identity = 298/685 (43.50%), Postives = 397/685 (57.96%), Query Frame = 1

Query: 14  VISLDVIDVDKDEASDAMFIHQKFDANDKGKGIYH-HGDHQLKKTLGSHSFNPDVEILES 73
           VI  DVID+D D++ D + I +K + ++KGK I   H DHQ+ KT       P VE   S
Sbjct: 32  VILHDVIDID-DDSEDVVIIGEKVNNSNKGKTIDAIHDDHQVVKTADYTYSLPAVENFGS 91

Query: 74  TNEVES--NFPSASHNLIDLDNPCSYSSNEDDDWLGI--DEFMDVDAYSALQAHFDHMDI 133
            + + S  NFPS S+NLI++D   S  S +DDD+  I  +++MDVD Y+ LQ HFD++DI
Sbjct: 92  VSGIASSNNFPSVSNNLINIDGHGSDLSYDDDDYFDILSEDYMDVDEYALLQKHFDNVDI 151

Query: 134 PPDIEAPIPWMTQD--------------------QNGNK---------------TNIETS 193
           P  IEAP  W+  D                     N NK               TN E  
Sbjct: 152 PTGIEAPFTWLPPDYVVGSKKTGNNTLYPWHHMQSNANKSPMTPSSQPSLSLEPTNSEIQ 211

Query: 194 RTSWSTLAKPQSVPGSVNHL-GKSTTQVQVKEVS----------NGLHLPADINVLNHLH 253
            +SW  +  P  + G+V+H  G   +   + E +           G    A + V +   
Sbjct: 212 ASSWGAINIPIKM-GNVDHSSGVELSSQYISEPAPSKKSATSKLRGRTSNASLGVESSKS 271

Query: 254 SFGSFPPQDIQPNKKPAAS------RRDKHELHNGGETS--DASYSVKKQVTSHGMPHPN 313
            + S P       KKPA++        +  ++ + GE        S KK+  S      +
Sbjct: 272 HWSSGP---FHSKKKPASTYHGFNYHAEAAKMPHAGELPYWGQFKSAKKEAGS------S 331

Query: 314 FPESVDFIEYLGAGH-----SMPQWPEYAKTKTKEPFFPPHMASGFYGSFNPFVPPTLGE 373
                 FIE+ G+ +     S   W E +           ++ +  Y  F+P V      
Sbjct: 332 ISSLSTFIEHHGSLYPPGIESGKHWWEISHNAKPFSTHLNNIPNHIYYPFDPLVAHP-EH 391

Query: 374 VVDVPWVQGSTQNLPNIAPNDSISEAIPPADKDKFLGNFENFKQFDTIDDHSDHHYASKG 433
           V D  WV  S ++  N    D     I    K++ L  F +FKQFDT++D SDHH+    
Sbjct: 392 VFDNNWVHDSARDGFNGKTVDGPIVTITDEAKEEILRKFRSFKQFDTVEDISDHHFFRSN 451

Query: 434 SSLNQASKKWTKKIQDDWKILQNDLPETIFVRVYESRMDLMRAVIIGAQGTPYHDGLFFF 493
           SS++Q  K W KKIQ++W+IL+ DLP +IFVRV+ESRMDL+RAVIIGA+GTPYHDGLFFF
Sbjct: 452 SSMHQPPKNWAKKIQEEWRILEKDLPASIFVRVFESRMDLLRAVIIGAEGTPYHDGLFFF 511

Query: 494 DIFFPPRYPDLPPQVYYHSKGLRLNPNLYNCGKVCLSLLNTWRGNGNENWVPGMSTMLQV 553
           D+FFP  YP++PP+V+YHS GLRLNPNLY CGKVCLSLLNTW G+ NE W+PG+ST+LQV
Sbjct: 512 DVFFPSAYPNVPPKVHYHSGGLRLNPNLYACGKVCLSLLNTWSGSKNEKWLPGVSTILQV 571

Query: 554 LVSIQGLILNTKPYFNEPGYAYQSGSEAGENRSHMYNEETYILSIKTMLFNIRRPPKHFE 613
           LVSIQGLILNTKPYFNEPGYA+ SGS  GE  S  YNE+T+ILS++TM++ IR+PPK+FE
Sbjct: 572 LVSIQGLILNTKPYFNEPGYAHMSGSANGEKMSFQYNEDTFILSLRTMMYMIRKPPKNFE 631

Query: 614 DFVRGHFYQRAHDILVACKAYINGAQVGSLVEGGVQDLELSDKSCSDSFKQSVTHLLSQL 635
           DFV+GHF  RA DILVACKAY+ GAQVG LV+GGVQD++  D+SCS  FK S++  ++ L
Sbjct: 632 DFVKGHFCSRASDILVACKAYMEGAQVGCLVKGGVQDVDEGDRSCSQRFKDSLSGYMNML 691

BLAST of Cucsa.123170 vs. TrEMBL
Match: I1M385_SOYBN (Uncharacterized protein OS=Glycine max GN=GLYMA_13G277400 PE=4 SV=2)

HSP 1 Score: 495.0 bits (1273), Expect = 1.4e-136
Identity = 297/698 (42.55%), Postives = 398/698 (57.02%), Query Frame = 1

Query: 2   IRRSSKMLKNKGVISLDVIDVDKDEASDAMFIHQKFDANDKGKGIYH-HGDHQLKKTLGS 61
           I    +  +   VI  DVID+D D++ D + I +K + ++KGK I   H DHQ+ KT   
Sbjct: 12  IHHPPRFREQNKVILHDVIDID-DDSEDVVIIGEKVNNSNKGKTIDAVHDDHQVVKTADY 71

Query: 62  HSFNPDVEILESTNEVES--NFPSASHNLIDLDNPCSYSSNEDDDWLGI--DEFMDVDAY 121
               P VE   S + + S  NFPS S+N+I++D   S  S +DDD++ I  +++MDVD Y
Sbjct: 72  TYSLPGVENFGSVSGIASSNNFPSVSNNMINIDGHGSDQSYDDDDYIDILSEDYMDVDEY 131

Query: 122 SALQAHFDHMDIPPDIEAPIPWMTQD--------------------QNGNKTNIE----- 181
           + LQ HFD++DIPP IEAP  W+  D                     N NK+ +      
Sbjct: 132 ALLQKHFDNVDIPPGIEAPFTWLPPDYVVGSKKTENSTLYPWHHMQSNANKSPMTPSSQP 191

Query: 182 ------TSRTSWSTLAKPQSVP---GSVNHLGKSTTQVQ---------VKEVSNGLHLPA 241
                 T+    ++L    ++P   G+V+H   +    Q         +K  ++ L    
Sbjct: 192 SLSLEPTNSKIQASLGSAINIPIKMGNVDHSSGAELSSQFFSEPAPSKMKSATSKLRGCT 251

Query: 242 DINVLNHLHSFGSFPPQDIQPNKKPAASRR------DKHELHNGGETS--DASYSVKKQV 301
               L    S   +  +     KKPA++        +  +L + GE        + KK+ 
Sbjct: 252 SNASLGVESSKSQWFSRPFHSKKKPASTYHGFNYHPEATKLPHAGELPYWGQFKTAKKEA 311

Query: 302 TSHGMPHPNFPESVDFIEYLGAGHSMPQWPEYAKTKTKEPFFPPHM---ASGFYGSFNPF 361
            S    H  F      +   G       W     TK     F  H+    +  Y  F+P 
Sbjct: 312 GSSISSHSTFIGHHGSLYPPGIESGKTWWKISHNTKP----FSTHLNNIPNHIYYPFDPL 371

Query: 362 VPPTLGEVVDVPWVQGSTQNLPNIAPNDSISEAIPPADKDKFLGNFENFKQFDTIDDHSD 421
           V      V D  WV  S ++  +    D     I    K++ L   ++FKQFDT++D SD
Sbjct: 372 VAHP-EHVFDNNWVNDSARDGFDGKTVDGPIVTISDEAKEEILRKLQSFKQFDTVEDTSD 431

Query: 422 HHYASKGSSLNQASKKWTKKIQDDWKILQNDLPETIFVRVYESRMDLMRAVIIGAQGTPY 481
           HH+    SS++Q  K W KKIQ++W+IL+ DLP +IFVRVYESRMDL+RAVIIGA+GTPY
Sbjct: 432 HHFFRSNSSMHQPPKNWAKKIQEEWRILEKDLPASIFVRVYESRMDLLRAVIIGAEGTPY 491

Query: 482 HDGLFFFDIFFPPRYPDLPPQVYYHSKGLRLNPNLYNCGKVCLSLLNTWRGNGNENWVPG 541
           HDGLFFFD+FFP  YP++PP+V+YHS GLRLNPNLY CGKVCLSLLNTW G+ NE WVPG
Sbjct: 492 HDGLFFFDVFFPSAYPNVPPKVHYHSGGLRLNPNLYACGKVCLSLLNTWSGSKNEKWVPG 551

Query: 542 MSTMLQVLVSIQGLILNTKPYFNEPGYAYQSGSEAGENRSHMYNEETYILSIKTMLFNIR 601
           MST+LQVLVSIQGLILNTKPYFNEPGYA+ SGS  GE  S  YNE+T+ILS++TM++ IR
Sbjct: 552 MSTILQVLVSIQGLILNTKPYFNEPGYAHMSGSANGEKMSFQYNEDTFILSLRTMMYMIR 611

Query: 602 RPPKHFEDFVRGHFYQRAHDILVACKAYINGAQVGSLVEGGVQDLELSDKSCSDSFKQSV 641
           +PPK+FED V+GHF  RA DILVACKAY+ GAQVG LV+GGVQD++  D+SCS  FK S+
Sbjct: 612 KPPKNFEDLVKGHFCNRASDILVACKAYMEGAQVGCLVKGGVQDVDEGDRSCSQQFKDSL 671

BLAST of Cucsa.123170 vs. TAIR10
Match: AT1G53025.1 (AT1G53025.1 Ubiquitin-conjugating enzyme family protein)

HSP 1 Score: 370.5 bits (950), Expect = 2.0e-102
Identity = 172/278 (61.87%), Postives = 217/278 (78.06%), Query Frame = 1

Query: 341 KDKFLGNFENFKQFDTIDDHSDHHYASKGSSLNQASKKWTKKIQDDWKILQNDLPETIFV 400
           K++FL    +FK+FDT++D SDHHYASKG S  Q SK W KK+Q DWKIL+NDLPE I V
Sbjct: 237 KEEFL---RDFKRFDTVEDFSDHHYASKGKSSKQHSKNWVKKVQADWKILENDLPEAISV 296

Query: 401 RVYESRMDLMRAVIIGAQGTPYHDGLFFFDIFFPPRYPDLPPQVYYHSKGLRLNPNLYNC 460
           R  ESRMDL+RAVIIGA+GTPYHDGLFFFDI FP  YP +PP V+YHS GLR+NPNLYNC
Sbjct: 297 RACESRMDLLRAVIIGAEGTPYHDGLFFFDIQFPDTYPSVPPNVHYHSGGLRINPNLYNC 356

Query: 461 GKVCLSLLNTWRGNGNENWVPGMSTMLQVLVSIQGLILNTKPYFNEPGYAYQSGSEAGEN 520
           GKVCLSLL TW G+  E W+P  STMLQ+LVSIQ LILN KPYFNEPGY   +G+ +GE+
Sbjct: 357 GKVCLSLLGTWAGSAREKWLPNESTMLQLLVSIQALILNEKPYFNEPGYVQSAGTASGES 416

Query: 521 RSHMYNEETYILSIKTMLFNIRRPPKHFEDFVRGHFYQRAHDILVACKAYINGAQVGSLV 580
           +S +Y+E  ++LS+KTM+++IRRPP+HFE++V+ H++ R+HDI+ AC AY  GA +GS+V
Sbjct: 417 KSKVYSENVFLLSLKTMVYSIRRPPQHFEEYVQNHYFVRSHDIVKACNAYKAGAPLGSMV 476

Query: 581 EGGVQDLELSDKSCSDSFKQSVTHLLSQLVAAFKGIGV 619
           +GGVQDLE + +S S  FK  V   +  +V  F  +GV
Sbjct: 477 KGGVQDLEEARQSGSKKFKTDVASFMQTVVDEFVKLGV 511

BLAST of Cucsa.123170 vs. TAIR10
Match: AT3G15355.1 (AT3G15355.1 ubiquitin-conjugating enzyme 25)

HSP 1 Score: 368.6 bits (945), Expect = 7.8e-102
Identity = 214/464 (46.12%), Postives = 275/464 (59.27%), Query Frame = 1

Query: 183 KEVSNGLHLPADINVLNHLHSFGSFPPQDIQPNKKPAASRRDKHELHNGGETSDASYSVK 242
           +++ +G  +P  + V     S   FP      +K+ A+S +  H  +NG  +S      K
Sbjct: 128 QDLFDGKDIPTGVEV-----SMDWFPNS---ADKESASSSKSSHA-NNGNNSS------K 187

Query: 243 KQVTSHGMPHPNFPESVDFIEYLGAGHSMPQWPE-------YAKTKTKEPFFPPH----- 302
           K   + G+ H  F   ++        +++P   E       YA  +  + F PP+     
Sbjct: 188 KATKASGI-HSQFSSDME-TPVAQPWNALPHKAEGVIPNSAYALPQNSKAFQPPYAVHYS 247

Query: 303 -MASGFYGSFNPFVPPTLGEVVDVPWVQGSTQNLPNIAPNDSISEA--------IPPADK 362
            + + F     P  P T+      P   GS+  LP   P    + A        +PP D 
Sbjct: 248 ALKTAFSNYLQPQTPDTVLGEAPAP-AAGSSGLLPPNTPGFKSNAARFKEEPPILPPDDS 307

Query: 363 ------DKFLGNFENFKQFDTIDDHSDHHYASKGSSLNQASKKWTKKIQDDWKILQNDLP 422
                 + +LG +  FK+FD ++D SDHHYASKG++  Q SK W K+IQD+W+IL+ DLP
Sbjct: 308 RVKRNMEDYLGLYLFFKRFDIVEDFSDHHYASKGTTSKQHSKDWAKRIQDEWRILEKDLP 367

Query: 423 ETIFVRVYESRMDLMRAVIIGAQGTPYHDGLFFFDIFFPPRYPDLPPQVYYHSKGLRLNP 482
           E IFVR YESRMDL+RAVIIGAQGTPYHDGLFFFDIFFP  YP  PP V+YHS GLR+NP
Sbjct: 368 EMIFVRAYESRMDLLRAVIIGAQGTPYHDGLFFFDIFFPDTYPSTPPIVHYHSGGLRINP 427

Query: 483 NLYNCGKVCLSLLNTWRGNGNENWVPGMSTMLQVLVSIQGLILNTKPYFNEPGYAYQSGS 542
           NLYNCGKVCLSLL TW GN  E W+P  STMLQVLVSIQGLILN KPYFNEPGY   +GS
Sbjct: 428 NLYNCGKVCLSLLGTWSGNQREKWIPNTSTMLQVLVSIQGLILNQKPYFNEPGYERSAGS 487

Query: 543 EAGENRSHMYNEETYILSIKTMLFNIRRPPKHFEDFVRGHFYQRAHDILVACKAYINGAQ 602
             GE+ S  Y+E T+ILS+KTM++ +RRPPK+FEDF  GHF+  AHD+L AC AY NGA 
Sbjct: 488 AHGESTSKAYSENTFILSLKTMVYTMRRPPKYFEDFAYGHFFSCAHDVLKACNAYRNGAT 547

Query: 603 VGSLVEGGVQDLELSDKSCSDSFKQSV-THLLSQLVAAFKGIGV 619
            G LV+G     E S    S  F+  V T + + L+  F  +GV
Sbjct: 548 PGYLVKGAPDVEENSAGMSSLKFRTDVATFVETVLLKEFILLGV 573

BLAST of Cucsa.123170 vs. TAIR10
Match: AT1G53023.1 (AT1G53023.1 Ubiquitin-conjugating enzyme family protein)

HSP 1 Score: 307.8 bits (787), Expect = 1.6e-83
Identity = 154/286 (53.85%), Postives = 199/286 (69.58%), Query Frame = 1

Query: 341 KDKFLGNFENFKQFDTIDDHSDHHYASKGSSLNQA---------SKKWTKKIQDDWKILQ 400
           K++FL    +FK+FDT++D SDHHYA++GS    +          K W K IQ +WKIL 
Sbjct: 14  KEEFL---RDFKRFDTVEDFSDHHYAAEGSPAKLSLWHLKFKGRPKNWVKDIQKEWKILD 73

Query: 401 NDLPETIFVRVYESRMDLMRAVIIGAQGTPYHDGLFFFDIFFPPRYPDLPPQVYYHSKGL 460
            +LPETIFVR  ESR+DL+RAVIIGA+GTPYHDGLFFFDI FP  YP +PP+V+YHS GL
Sbjct: 74  KNLPETIFVRACESRIDLLRAVIIGAEGTPYHDGLFFFDIQFPDTYPSVPPKVHYHSGGL 133

Query: 461 RLNPNLYNCGKVCLSLLNTWRGNGNENWVPGMSTMLQVLVSIQGLILNTKPYFNEPGYAY 520
           R+NPNLY CGKVCLSL++TW G   E W+P  STMLQ+LVSIQ LILN KPY+NEPGY  
Sbjct: 134 RINPNLYKCGKVCLSLISTWTGKKREKWLPKESTMLQLLVSIQALILNEKPYYNEPGYEK 193

Query: 521 QSGSEAGENRSHMYNEETYILSIKTMLFNIRRPPKHFEDFVRGHFYQRAHDILVACKAYI 580
             G+  GE+ S  Y+E  ++ S+KTM         HFE+FVR HF+ R+HDI+ AC AY 
Sbjct: 194 SMGTPLGESYSKDYSENVFVFSLKTM---------HFEEFVRSHFFVRSHDIVKACNAYK 253

Query: 581 NGAQVGSLVEGGVQDLELSDKSCSDSFKQSVTHLLSQLVAAFKGIG 618
           +GA VGS+ +GGV+  + + +  S  F+ +VT  +  +V  F  +G
Sbjct: 254 DGAPVGSIDKGGVK--KQTRQRGSLKFRINVTSFMKTVVDEFVNLG 285

BLAST of Cucsa.123170 vs. TAIR10
Match: AT2G33770.1 (AT2G33770.1 phosphate 2)

HSP 1 Score: 297.7 bits (761), Expect = 1.7e-80
Identity = 145/277 (52.35%), Postives = 189/277 (68.23%), Query Frame = 1

Query: 347 NFENFKQFDTIDDHSDHHYAS--KGSSLNQASKKWTKKIQDDWKILQNDLPETIFVRVYE 406
           N   F+QFD ++D SDHH+ S  KG + +Q +K W KK+Q +W  L+ +LP TI+VRV E
Sbjct: 629 NQVRFRQFDMVNDCSDHHFLSSDKGLAQSQVTKSWVKKVQQEWSNLEANLPNTIYVRVCE 688

Query: 407 SRMDLMRAVIIGAQGTPYHDGLFFFDIFFPPRYPDLPPQVYYHSKGLRLNPNLYNCGKVC 466
            RMDL+RA ++GA GTPYHDGLFFFDI  PP+YP  PP V+YHS G+RLNPNLY  G+VC
Sbjct: 689 ERMDLLRAALVGAPGTPYHDGLFFFDIMLPPQYPHEPPMVHYHSGGMRLNPNLYESGRVC 748

Query: 467 LSLLNTWRGNGNENWVPGMSTMLQVLVSIQGLILNTKPYFNEPGYAYQSGSEAGENRSHM 526
           LSLLNTW G+G E W  G S++LQ+L+S Q L+LN KPYFNE GY  Q G   GE  S  
Sbjct: 749 LSLLNTWSGSGTEVWNAGSSSILQLLLSFQALVLNEKPYFNEAGYDKQLGRAEGEKNSVS 808

Query: 527 YNEETYILSIKTMLFNIRRPPKHFEDFVRGHFYQRAHDILVACKAYINGAQVGSLVEGGV 586
           YNE  ++++ K+M+  +R+PPKHFE  V+ HF  RA  +L ACKAY+ G  VGS      
Sbjct: 809 YNENAFLITCKSMISMLRKPPKHFEMLVKDHFTHRAQHVLAACKAYMEGVPVGSSA---- 868

Query: 587 QDLELSDKSCSDSFKQSVTHLLSQLVAAFKGIGVGDC 622
            +L+ +  + S  FK  ++ L  +L+ AF  IGV DC
Sbjct: 869 -NLQGNSTTNSTGFKIMLSKLYPKLLEAFSEIGV-DC 899

BLAST of Cucsa.123170 vs. TAIR10
Match: AT2G16920.1 (AT2G16920.1 ubiquitin-conjugating enzyme 23)

HSP 1 Score: 295.8 bits (756), Expect = 6.4e-80
Identity = 155/324 (47.84%), Postives = 206/324 (63.58%), Query Frame = 1

Query: 305  PTLGEVVDVPWVQGSTQNLPNIAPNDSISEAIPPADKDKFL---GNFENFKQFDTIDDHS 364
            P+   V D    +G+  N  NI   ++ +      D+DK +   G+  +F++FD   D  
Sbjct: 778  PSKVNVTDNCESKGTQANAKNILSGETSTFL---EDEDKPVPSEGDSCSFRRFDISQDPL 837

Query: 365  DHHYASKGSSLNQASKKWTKKIQDDWKILQNDLPETIFVRVYESRMDLMRAVIIGAQGTP 424
            DHH+        +  ++W KK+  DWKILQN+LP+ IFVR YE RMDL+RAVI+GA GTP
Sbjct: 838  DHHFLGVDGQKTK-ERQWFKKVDQDWKILQNNLPDGIFVRAYEDRMDLLRAVIVGAFGTP 897

Query: 425  YHDGLFFFDIFFPPRYPDLPPQVYYHSKGLRLNPNLYNCGKVCLSLLNTWRGNGNENWVP 484
            Y DGLFFFD   P  YP +PP  YYHS G RLNPNLY  GKVCLSLLNTW G GNE W P
Sbjct: 898  YQDGLFFFDFHLPSDYPSVPPSAYYHSGGWRLNPNLYEEGKVCLSLLNTWTGRGNEVWDP 957

Query: 485  GMSTMLQVLVSIQGLILNTKPYFNEPGYAYQSGSEAGENRSHMYNEETYILSIKTMLFNI 544
              S++LQVLVS+QGL+LN+KPYFNE GY  Q G+  GE  S  YNE T++L+ KTM++ +
Sbjct: 958  KSSSILQVLVSLQGLVLNSKPYFNEAGYDKQVGTAEGEKNSLGYNENTFLLNCKTMMYLM 1017

Query: 545  RRPPKHFEDFVRGHFYQRAHDILVACKAYINGAQVGSLV-EGGVQDLELSDKSCSDSFKQ 604
            R+PPK FE+ ++ HF +R + IL AC AY+ G  +GSL  +  V D   S  S S  FK 
Sbjct: 1018 RKPPKDFEELIKDHFRKRGYYILKACDAYMKGYLIGSLTKDASVIDERSSANSTSVGFKL 1077

Query: 605  SVTHLLSQLVAAFKGIGVGDCDKY 625
             +  +  +L +A   +G  DC+++
Sbjct: 1078 MLAKIAPKLFSALSEVG-ADCNEF 1096

BLAST of Cucsa.123170 vs. NCBI nr
Match: gi|449447213|ref|XP_004141363.1| (PREDICTED: probable ubiquitin-conjugating enzyme E2 25 isoform X2 [Cucumis sativus])

HSP 1 Score: 1344.3 bits (3478), Expect = 0.0e+00
Identity = 645/646 (99.85%), Postives = 645/646 (99.85%), Query Frame = 1

Query: 1   MIRRSSKMLKNKGVISLDVIDVDKDEASDAMFIHQKFDANDKGKGIYHHGDHQLKKTLGS 60
           MIRRSSKMLKNKGVISLDVIDVDKDEASDAMFIHQKFDANDKGKGIYHHGDHQLKKTLGS
Sbjct: 1   MIRRSSKMLKNKGVISLDVIDVDKDEASDAMFIHQKFDANDKGKGIYHHGDHQLKKTLGS 60

Query: 61  HSFNPDVEILESTNEVESNFPSASHNLIDLDNPCSYSSNEDDDWLGIDEFMDVDAYSALQ 120
           HSFNPDVEILESTNEVESNFPSASHNLIDLDNPCSYSSNEDDDWLGIDEFMDVDAYSALQ
Sbjct: 61  HSFNPDVEILESTNEVESNFPSASHNLIDLDNPCSYSSNEDDDWLGIDEFMDVDAYSALQ 120

Query: 121 AHFDHMDIPPDIEAPIPWMTQDQNGNKTNIETSRTSWSTLAKPQSVPGSVNHLGKSTTQV 180
           AHFDHMDIPPDIEAPIPWMTQDQNGNKTNIETSRTSWSTLAKPQSVPGSVNHLGKSTTQV
Sbjct: 121 AHFDHMDIPPDIEAPIPWMTQDQNGNKTNIETSRTSWSTLAKPQSVPGSVNHLGKSTTQV 180

Query: 181 QVKEVSNGLHLPADINVLNHLHSFGSFPPQDIQPNKKPAASRRDKHELHNGGETSDASYS 240
           QVKEVSNGLHLPADINVLNHLHSFGSFPPQDIQPNKKPAASRRDKHELHNGGETSDASYS
Sbjct: 181 QVKEVSNGLHLPADINVLNHLHSFGSFPPQDIQPNKKPAASRRDKHELHNGGETSDASYS 240

Query: 241 VKKQVTSHGMPHPNFPESVDFIEYLGAGHSMPQWPEYAKTKTKEPFFPPHMASGFYGSFN 300
           VKKQVTSHGMPHPNFPESVDFIEYLGAGHSMPQWPEYAKTKTKEPFFPPHMASGFYGSFN
Sbjct: 241 VKKQVTSHGMPHPNFPESVDFIEYLGAGHSMPQWPEYAKTKTKEPFFPPHMASGFYGSFN 300

Query: 301 PFVPPTLGEVVDVPWVQGSTQNLPNIAPNDSISEAIPPADKDKFLGNFENFKQFDTIDDH 360
           PFVPPTLGEVVDVPWVQGSTQNLPNIAPNDSISEAIPPADKDKFLGNFENFKQFDTIDDH
Sbjct: 301 PFVPPTLGEVVDVPWVQGSTQNLPNIAPNDSISEAIPPADKDKFLGNFENFKQFDTIDDH 360

Query: 361 SDHHYASKGSSLNQASKKWTKKIQDDWKILQNDLPETIFVRVYESRMDLMRAVIIGAQGT 420
           SDHHYASKGSSLNQASKKWTKKIQDDWKILQNDLPETIFVRVYESRMDLMRAVIIGAQGT
Sbjct: 361 SDHHYASKGSSLNQASKKWTKKIQDDWKILQNDLPETIFVRVYESRMDLMRAVIIGAQGT 420

Query: 421 PYHDGLFFFDIFFPPRYPDLPPQVYYHSKGLRLNPNLYNCGKVCLSLLNTWRGNGNENWV 480
           PYHDGLFFFDIFFPPRYPDLPPQVYYHSKGLRLNPNLYNCGKVCLSLLNTWRGNGNENWV
Sbjct: 421 PYHDGLFFFDIFFPPRYPDLPPQVYYHSKGLRLNPNLYNCGKVCLSLLNTWRGNGNENWV 480

Query: 481 PGMSTMLQVLVSIQGLILNTKPYFNEPGYAYQSGSEAGENRSHMYNEETYILSIKTMLFN 540
           PGMSTMLQVLVSIQGLILNTKPYFNEPGYAYQSGSEAGENRSHMYNEETYILSIKTMLFN
Sbjct: 481 PGMSTMLQVLVSIQGLILNTKPYFNEPGYAYQSGSEAGENRSHMYNEETYILSIKTMLFN 540

Query: 541 IRRPPKHFEDFVRGHFYQRAHDILVACKAYINGAQVGSLVEGGVQDLELSDKSCSDSFKQ 600
           IRRPPKHFEDFVRGHFYQRAHDILVACKAYINGAQVGSLVEGGVQDLELSDKSCSDSFKQ
Sbjct: 541 IRRPPKHFEDFVRGHFYQRAHDILVACKAYINGAQVGSLVEGGVQDLELSDKSCSDSFKQ 600

Query: 601 SVTHLLSQLVAAFKGIGVGDCDKYLQMLSPTVPNTRKRPSRPVKLK 647
           SVTHLLSQLVAAFKGIGV DCDKYLQMLSPTVPNTRKRPSRPVKLK
Sbjct: 601 SVTHLLSQLVAAFKGIGVSDCDKYLQMLSPTVPNTRKRPSRPVKLK 646

BLAST of Cucsa.123170 vs. NCBI nr
Match: gi|659103082|ref|XP_008452464.1| (PREDICTED: probable ubiquitin-conjugating enzyme E2 25 isoform X3 [Cucumis melo])

HSP 1 Score: 1307.7 bits (3383), Expect = 0.0e+00
Identity = 627/647 (96.91%), Postives = 635/647 (98.15%), Query Frame = 1

Query: 1   MIRRSSKMLKNKGVISLDVIDVDKDEAS-DAMFIHQKFDANDKGKGIYHHGDHQLKKTLG 60
           MIRRSSKMLKNKGVISLDVIDVDKDEAS  AMFIHQKFDANDKGKG+YHHGDHQLKKTLG
Sbjct: 33  MIRRSSKMLKNKGVISLDVIDVDKDEASGSAMFIHQKFDANDKGKGVYHHGDHQLKKTLG 92

Query: 61  SHSFNPDVEILESTNEVESNFPSASHNLIDLDNPCSYSSNEDDDWLGIDEFMDVDAYSAL 120
           SHSFNPDVEILESTNEVESNFPSASHNLIDLDNPCSYSSNEDDDWLGIDEFMDVDAYSAL
Sbjct: 93  SHSFNPDVEILESTNEVESNFPSASHNLIDLDNPCSYSSNEDDDWLGIDEFMDVDAYSAL 152

Query: 121 QAHFDHMDIPPDIEAPIPWMTQDQNGNKTNIETSRTSWSTLAKPQSVPGSVNHLGKSTTQ 180
           QAHFDHMDIPPDIEAPIPWMTQDQNGNKTNIETSRTSWSTLAKPQSVPGSVNHLGKSTTQ
Sbjct: 153 QAHFDHMDIPPDIEAPIPWMTQDQNGNKTNIETSRTSWSTLAKPQSVPGSVNHLGKSTTQ 212

Query: 181 VQVKEVSNGLHLPADINVLNHLHSFGSFPPQDIQPNKKPAASRRDKHELHNGGETSDASY 240
           VQVKEVSN LHLPADINVLNHLH FGSFPPQDIQPNKK A+SR DKHELHNGGETSDASY
Sbjct: 213 VQVKEVSNNLHLPADINVLNHLHPFGSFPPQDIQPNKKSASSRWDKHELHNGGETSDASY 272

Query: 241 SVKKQVTSHGMPHPNFPESVDFIEYLGAGHSMPQWPEYAKTKTKEPFFPPHMASGFYGSF 300
           SVKKQVTSH MPHPNFPESVDFIEYLGAG SMPQWPEYAKTKTKEPFFPPHMASGFYGSF
Sbjct: 273 SVKKQVTSHSMPHPNFPESVDFIEYLGAGKSMPQWPEYAKTKTKEPFFPPHMASGFYGSF 332

Query: 301 NPFVPPTLGEVVDVPWVQGSTQNLPNIAPNDSISEAIPPADKDKFLGNFENFKQFDTIDD 360
           NPFVPPTLGEVVDVPWVQGSTQNL NIAPNDSISEAIPPADKDKFLGNFE+FKQFDT+DD
Sbjct: 333 NPFVPPTLGEVVDVPWVQGSTQNLSNIAPNDSISEAIPPADKDKFLGNFESFKQFDTVDD 392

Query: 361 HSDHHYASKGSSLNQASKKWTKKIQDDWKILQNDLPETIFVRVYESRMDLMRAVIIGAQG 420
           HSDHHYA+KGSSLNQASKKWTKKIQDDWKILQNDLPETIFVRVYESRMDLMRAVIIGAQG
Sbjct: 393 HSDHHYATKGSSLNQASKKWTKKIQDDWKILQNDLPETIFVRVYESRMDLMRAVIIGAQG 452

Query: 421 TPYHDGLFFFDIFFPPRYPDLPPQVYYHSKGLRLNPNLYNCGKVCLSLLNTWRGNGNENW 480
           TPYHDGLFFFDIFFPPRYPDLPPQVYYHSKGLRLNPNLYNCGKVCLSLLNTWRGNGNENW
Sbjct: 453 TPYHDGLFFFDIFFPPRYPDLPPQVYYHSKGLRLNPNLYNCGKVCLSLLNTWRGNGNENW 512

Query: 481 VPGMSTMLQVLVSIQGLILNTKPYFNEPGYAYQSGSEAGENRSHMYNEETYILSIKTMLF 540
           VPGMSTMLQVLVSIQGLILNTKPYFNEPGYAYQSGSEAGENRSHMYNEETYILSIKTMLF
Sbjct: 513 VPGMSTMLQVLVSIQGLILNTKPYFNEPGYAYQSGSEAGENRSHMYNEETYILSIKTMLF 572

Query: 541 NIRRPPKHFEDFVRGHFYQRAHDILVACKAYINGAQVGSLVEGGVQDLELSDKSCSDSFK 600
           NIRRPPKHFEDFVRGHFY+RAHDILVACKAYINGAQVGSLVEGGVQDLELSDKSCSDSFK
Sbjct: 573 NIRRPPKHFEDFVRGHFYKRAHDILVACKAYINGAQVGSLVEGGVQDLELSDKSCSDSFK 632

Query: 601 QSVTHLLSQLVAAFKGIGVGDCDKYLQMLSPTVPNTRKRPSRPVKLK 647
           QSVTHLLSQLV AFKGIGVGDCDKYLQMLSPT+PN RKRP RPVK+K
Sbjct: 633 QSVTHLLSQLVGAFKGIGVGDCDKYLQMLSPTIPNARKRPGRPVKIK 679

BLAST of Cucsa.123170 vs. NCBI nr
Match: gi|778695935|ref|XP_011654074.1| (PREDICTED: probable ubiquitin-conjugating enzyme E2 25 isoform X1 [Cucumis sativus])

HSP 1 Score: 1250.7 bits (3235), Expect = 0.0e+00
Identity = 619/689 (89.84%), Postives = 629/689 (91.29%), Query Frame = 1

Query: 1   MIRRSSKMLKNKGVISLDVIDVDKDEASDAMFIHQKFDANDKGKGIYHHGDHQLKKTLGS 60
           MIRRSSKMLKNKGVISLDVIDVDKDEASDAMFIHQKFDANDKGKGIYHHGDHQLKKTLGS
Sbjct: 1   MIRRSSKMLKNKGVISLDVIDVDKDEASDAMFIHQKFDANDKGKGIYHHGDHQLKKTLGS 60

Query: 61  HSFNPDVEILESTNEVESNFPSASHNLIDLDNPCSYSSNEDDDWLGIDEFMDVDAYSALQ 120
           HSFNPDVEILESTNEVESNFPSASHNLIDLDNPCSYSSNEDDDWLGIDEFMDVDAYSALQ
Sbjct: 61  HSFNPDVEILESTNEVESNFPSASHNLIDLDNPCSYSSNEDDDWLGIDEFMDVDAYSALQ 120

Query: 121 AHFDHMDIPPDIEAPIPWMTQDQNGNKTNIETSRTSWSTLAKPQSVPGSVNHLGKSTTQV 180
           AHFDHMDIPPDIEAPIPWMTQDQNGNKTNIETSRTSWSTLAKPQSVPGSVNHLGKSTTQV
Sbjct: 121 AHFDHMDIPPDIEAPIPWMTQDQNGNKTNIETSRTSWSTLAKPQSVPGSVNHLGKSTTQV 180

Query: 181 QVKEVSNGLHL-----------------PADI-------------NVLNHLHSF-GSFPP 240
           QVKEVSNGLHL                 P DI             + ++HL+S  G +  
Sbjct: 181 QVKEVSNGLHLPADINVLNHLHSFGSFPPQDIQPNKKPAASRRDKHEVSHLNSLLGIYAA 240

Query: 241 QD---IQPNKKP---------AASRRDKHELHNGGETSDASYSVKKQVTSHGMPHPNFPE 300
           +    + P K           + +  D  +LHNGGETSDASYSVKKQVTSHGMPHPNFPE
Sbjct: 241 KSRWFLDPFKHKKKLHGSNNISINSSDAMKLHNGGETSDASYSVKKQVTSHGMPHPNFPE 300

Query: 301 SVDFIEYLGAGHSMPQWPEYAKTKTKEPFFPPHMASGFYGSFNPFVPPTLGEVVDVPWVQ 360
           SVDFIEYLGAGHSMPQWPEYAKTKTKEPFFPPHMASGFYGSFNPFVPPTLGEVVDVPWVQ
Sbjct: 301 SVDFIEYLGAGHSMPQWPEYAKTKTKEPFFPPHMASGFYGSFNPFVPPTLGEVVDVPWVQ 360

Query: 361 GSTQNLPNIAPNDSISEAIPPADKDKFLGNFENFKQFDTIDDHSDHHYASKGSSLNQASK 420
           GSTQNLPNIAPNDSISEAIPPADKDKFLGNFENFKQFDTIDDHSDHHYASKGSSLNQASK
Sbjct: 361 GSTQNLPNIAPNDSISEAIPPADKDKFLGNFENFKQFDTIDDHSDHHYASKGSSLNQASK 420

Query: 421 KWTKKIQDDWKILQNDLPETIFVRVYESRMDLMRAVIIGAQGTPYHDGLFFFDIFFPPRY 480
           KWTKKIQDDWKILQNDLPETIFVRVYESRMDLMRAVIIGAQGTPYHDGLFFFDIFFPPRY
Sbjct: 421 KWTKKIQDDWKILQNDLPETIFVRVYESRMDLMRAVIIGAQGTPYHDGLFFFDIFFPPRY 480

Query: 481 PDLPPQVYYHSKGLRLNPNLYNCGKVCLSLLNTWRGNGNENWVPGMSTMLQVLVSIQGLI 540
           PDLPPQVYYHSKGLRLNPNLYNCGKVCLSLLNTWRGNGNENWVPGMSTMLQVLVSIQGLI
Sbjct: 481 PDLPPQVYYHSKGLRLNPNLYNCGKVCLSLLNTWRGNGNENWVPGMSTMLQVLVSIQGLI 540

Query: 541 LNTKPYFNEPGYAYQSGSEAGENRSHMYNEETYILSIKTMLFNIRRPPKHFEDFVRGHFY 600
           LNTKPYFNEPGYAYQSGSEAGENRSHMYNEETYILSIKTMLFNIRRPPKHFEDFVRGHFY
Sbjct: 541 LNTKPYFNEPGYAYQSGSEAGENRSHMYNEETYILSIKTMLFNIRRPPKHFEDFVRGHFY 600

Query: 601 QRAHDILVACKAYINGAQVGSLVEGGVQDLELSDKSCSDSFKQSVTHLLSQLVAAFKGIG 647
           QRAHDILVACKAYINGAQVGSLVEGGVQDLELSDKSCSDSFKQSVTHLLSQLVAAFKGIG
Sbjct: 601 QRAHDILVACKAYINGAQVGSLVEGGVQDLELSDKSCSDSFKQSVTHLLSQLVAAFKGIG 660

BLAST of Cucsa.123170 vs. NCBI nr
Match: gi|659103080|ref|XP_008452463.1| (PREDICTED: probable ubiquitin-conjugating enzyme E2 25 isoform X2 [Cucumis melo])

HSP 1 Score: 1223.4 bits (3164), Expect = 0.0e+00
Identity = 604/690 (87.54%), Postives = 620/690 (89.86%), Query Frame = 1

Query: 1   MIRRSSKMLKNKGVISLDVIDVDKDEAS-DAMFIHQKFDANDKGKGIYHHGDHQLKKTLG 60
           MIRRSSKMLKNKGVISLDVIDVDKDEAS  AMFIHQKFDANDKGKG+YHHGDHQLKKTLG
Sbjct: 1   MIRRSSKMLKNKGVISLDVIDVDKDEASGSAMFIHQKFDANDKGKGVYHHGDHQLKKTLG 60

Query: 61  SHSFNPDVEILESTNEVESNFPSASHNLIDLDNPCSYSSNEDDDWLGIDEFMDVDAYSAL 120
           SHSFNPDVEILESTNEVESNFPSASHNLIDLDNPCSYSSNEDDDWLGIDEFMDVDAYSAL
Sbjct: 61  SHSFNPDVEILESTNEVESNFPSASHNLIDLDNPCSYSSNEDDDWLGIDEFMDVDAYSAL 120

Query: 121 QAHFDHMDIPPDIEAPIPWMTQDQNGNKTNIETSRTSWSTLAKPQSVPGSVNHLGKSTTQ 180
           QAHFDHMDIPPDIEAPIPWMTQDQNGNKTNIETSRTSWSTLAKPQSVPGSVNHLGKSTTQ
Sbjct: 121 QAHFDHMDIPPDIEAPIPWMTQDQNGNKTNIETSRTSWSTLAKPQSVPGSVNHLGKSTTQ 180

Query: 181 VQVKEVSNGLHL-----------------PADI-------------NVLNHLHSFGSFPP 240
           VQVKEVSN LHL                 P DI             + ++HL+S      
Sbjct: 181 VQVKEVSNNLHLPADINVLNHLHPFGSFPPQDIQPNKKSASSRWDKHEVSHLNSLLGIDA 240

Query: 241 QD----IQPNKKP---------AASRRDKHELHNGGETSDASYSVKKQVTSHGMPHPNFP 300
                 + P K+          + +  D  +LHNGGETSDASYSVKKQVTSH MPHPNFP
Sbjct: 241 AKSRWFLDPFKRKKKLHGSNNISINSSDAMKLHNGGETSDASYSVKKQVTSHSMPHPNFP 300

Query: 301 ESVDFIEYLGAGHSMPQWPEYAKTKTKEPFFPPHMASGFYGSFNPFVPPTLGEVVDVPWV 360
           ESVDFIEYLGAG SMPQWPEYAKTKTKEPFFPPHMASGFYGSFNPFVPPTLGEVVDVPWV
Sbjct: 301 ESVDFIEYLGAGKSMPQWPEYAKTKTKEPFFPPHMASGFYGSFNPFVPPTLGEVVDVPWV 360

Query: 361 QGSTQNLPNIAPNDSISEAIPPADKDKFLGNFENFKQFDTIDDHSDHHYASKGSSLNQAS 420
           QGSTQNL NIAPNDSISEAIPPADKDKFLGNFE+FKQFDT+DDHSDHHYA+KGSSLNQAS
Sbjct: 361 QGSTQNLSNIAPNDSISEAIPPADKDKFLGNFESFKQFDTVDDHSDHHYATKGSSLNQAS 420

Query: 421 KKWTKKIQDDWKILQNDLPETIFVRVYESRMDLMRAVIIGAQGTPYHDGLFFFDIFFPPR 480
           KKWTKKIQDDWKILQNDLPETIFVRVYESRMDLMRAVIIGAQGTPYHDGLFFFDIFFPPR
Sbjct: 421 KKWTKKIQDDWKILQNDLPETIFVRVYESRMDLMRAVIIGAQGTPYHDGLFFFDIFFPPR 480

Query: 481 YPDLPPQVYYHSKGLRLNPNLYNCGKVCLSLLNTWRGNGNENWVPGMSTMLQVLVSIQGL 540
           YPDLPPQVYYHSKGLRLNPNLYNCGKVCLSLLNTWRGNGNENWVPGMSTMLQVLVSIQGL
Sbjct: 481 YPDLPPQVYYHSKGLRLNPNLYNCGKVCLSLLNTWRGNGNENWVPGMSTMLQVLVSIQGL 540

Query: 541 ILNTKPYFNEPGYAYQSGSEAGENRSHMYNEETYILSIKTMLFNIRRPPKHFEDFVRGHF 600
           ILNTKPYFNEPGYAYQSGSEAGENRSHMYNEETYILSIKTMLFNIRRPPKHFEDFVRGHF
Sbjct: 541 ILNTKPYFNEPGYAYQSGSEAGENRSHMYNEETYILSIKTMLFNIRRPPKHFEDFVRGHF 600

Query: 601 YQRAHDILVACKAYINGAQVGSLVEGGVQDLELSDKSCSDSFKQSVTHLLSQLVAAFKGI 647
           Y+RAHDILVACKAYINGAQVGSLVEGGVQDLELSDKSCSDSFKQSVTHLLSQLV AFKGI
Sbjct: 601 YKRAHDILVACKAYINGAQVGSLVEGGVQDLELSDKSCSDSFKQSVTHLLSQLVGAFKGI 660

BLAST of Cucsa.123170 vs. NCBI nr
Match: gi|659103078|ref|XP_008452462.1| (PREDICTED: probable ubiquitin-conjugating enzyme E2 25 isoform X1 [Cucumis melo])

HSP 1 Score: 1223.4 bits (3164), Expect = 0.0e+00
Identity = 604/690 (87.54%), Postives = 620/690 (89.86%), Query Frame = 1

Query: 1   MIRRSSKMLKNKGVISLDVIDVDKDEAS-DAMFIHQKFDANDKGKGIYHHGDHQLKKTLG 60
           MIRRSSKMLKNKGVISLDVIDVDKDEAS  AMFIHQKFDANDKGKG+YHHGDHQLKKTLG
Sbjct: 33  MIRRSSKMLKNKGVISLDVIDVDKDEASGSAMFIHQKFDANDKGKGVYHHGDHQLKKTLG 92

Query: 61  SHSFNPDVEILESTNEVESNFPSASHNLIDLDNPCSYSSNEDDDWLGIDEFMDVDAYSAL 120
           SHSFNPDVEILESTNEVESNFPSASHNLIDLDNPCSYSSNEDDDWLGIDEFMDVDAYSAL
Sbjct: 93  SHSFNPDVEILESTNEVESNFPSASHNLIDLDNPCSYSSNEDDDWLGIDEFMDVDAYSAL 152

Query: 121 QAHFDHMDIPPDIEAPIPWMTQDQNGNKTNIETSRTSWSTLAKPQSVPGSVNHLGKSTTQ 180
           QAHFDHMDIPPDIEAPIPWMTQDQNGNKTNIETSRTSWSTLAKPQSVPGSVNHLGKSTTQ
Sbjct: 153 QAHFDHMDIPPDIEAPIPWMTQDQNGNKTNIETSRTSWSTLAKPQSVPGSVNHLGKSTTQ 212

Query: 181 VQVKEVSNGLHL-----------------PADI-------------NVLNHLHSFGSFPP 240
           VQVKEVSN LHL                 P DI             + ++HL+S      
Sbjct: 213 VQVKEVSNNLHLPADINVLNHLHPFGSFPPQDIQPNKKSASSRWDKHEVSHLNSLLGIDA 272

Query: 241 QD----IQPNKKP---------AASRRDKHELHNGGETSDASYSVKKQVTSHGMPHPNFP 300
                 + P K+          + +  D  +LHNGGETSDASYSVKKQVTSH MPHPNFP
Sbjct: 273 AKSRWFLDPFKRKKKLHGSNNISINSSDAMKLHNGGETSDASYSVKKQVTSHSMPHPNFP 332

Query: 301 ESVDFIEYLGAGHSMPQWPEYAKTKTKEPFFPPHMASGFYGSFNPFVPPTLGEVVDVPWV 360
           ESVDFIEYLGAG SMPQWPEYAKTKTKEPFFPPHMASGFYGSFNPFVPPTLGEVVDVPWV
Sbjct: 333 ESVDFIEYLGAGKSMPQWPEYAKTKTKEPFFPPHMASGFYGSFNPFVPPTLGEVVDVPWV 392

Query: 361 QGSTQNLPNIAPNDSISEAIPPADKDKFLGNFENFKQFDTIDDHSDHHYASKGSSLNQAS 420
           QGSTQNL NIAPNDSISEAIPPADKDKFLGNFE+FKQFDT+DDHSDHHYA+KGSSLNQAS
Sbjct: 393 QGSTQNLSNIAPNDSISEAIPPADKDKFLGNFESFKQFDTVDDHSDHHYATKGSSLNQAS 452

Query: 421 KKWTKKIQDDWKILQNDLPETIFVRVYESRMDLMRAVIIGAQGTPYHDGLFFFDIFFPPR 480
           KKWTKKIQDDWKILQNDLPETIFVRVYESRMDLMRAVIIGAQGTPYHDGLFFFDIFFPPR
Sbjct: 453 KKWTKKIQDDWKILQNDLPETIFVRVYESRMDLMRAVIIGAQGTPYHDGLFFFDIFFPPR 512

Query: 481 YPDLPPQVYYHSKGLRLNPNLYNCGKVCLSLLNTWRGNGNENWVPGMSTMLQVLVSIQGL 540
           YPDLPPQVYYHSKGLRLNPNLYNCGKVCLSLLNTWRGNGNENWVPGMSTMLQVLVSIQGL
Sbjct: 513 YPDLPPQVYYHSKGLRLNPNLYNCGKVCLSLLNTWRGNGNENWVPGMSTMLQVLVSIQGL 572

Query: 541 ILNTKPYFNEPGYAYQSGSEAGENRSHMYNEETYILSIKTMLFNIRRPPKHFEDFVRGHF 600
           ILNTKPYFNEPGYAYQSGSEAGENRSHMYNEETYILSIKTMLFNIRRPPKHFEDFVRGHF
Sbjct: 573 ILNTKPYFNEPGYAYQSGSEAGENRSHMYNEETYILSIKTMLFNIRRPPKHFEDFVRGHF 632

Query: 601 YQRAHDILVACKAYINGAQVGSLVEGGVQDLELSDKSCSDSFKQSVTHLLSQLVAAFKGI 647
           Y+RAHDILVACKAYINGAQVGSLVEGGVQDLELSDKSCSDSFKQSVTHLLSQLV AFKGI
Sbjct: 633 YKRAHDILVACKAYINGAQVGSLVEGGVQDLELSDKSCSDSFKQSVTHLLSQLVGAFKGI 692

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
UBC26_ARATH3.6e-10161.87Probable ubiquitin-conjugating enzyme E2 26 OS=Arabidopsis thaliana GN=UBC26 PE=... [more]
UBC25_ARATH1.4e-10046.12Probable ubiquitin-conjugating enzyme E2 25 OS=Arabidopsis thaliana GN=UBC25 PE=... [more]
UBC38_ARATH5.2e-10060.43Putative ubiquitin-conjugating enzyme E2 38 OS=Arabidopsis thaliana GN=UBC38 PE=... [more]
UBC39_ARATH2.9e-8253.85Putative ubiquitin-conjugating enzyme E2 39 OS=Arabidopsis thaliana GN=UBC39 PE=... [more]
UBC24_ARATH3.0e-7952.35Probable ubiquitin-conjugating enzyme E2 24 OS=Arabidopsis thaliana GN=UBC24 PE=... [more]
Match NameE-valueIdentityDescription
A0A0A0KZM7_CUCSA0.0e+0099.85Uncharacterized protein OS=Cucumis sativus GN=Csa_4G637700 PE=4 SV=1[more]
A0A061F7Z1_THECC2.5e-14145.04Ubiquitin-conjugating enzyme 25, putative isoform 1 OS=Theobroma cacao GN=TCM_03... [more]
I1LV04_SOYBN4.9e-13742.90Uncharacterized protein OS=Glycine max GN=GLYMA_12G224200 PE=4 SV=2[more]
A0A0B2R8C8_GLYSO6.4e-13743.50Putative ubiquitin-conjugating enzyme E2 26 OS=Glycine soja GN=glysoja_007789 PE... [more]
I1M385_SOYBN1.4e-13642.55Uncharacterized protein OS=Glycine max GN=GLYMA_13G277400 PE=4 SV=2[more]
Match NameE-valueIdentityDescription
AT1G53025.12.0e-10261.87 Ubiquitin-conjugating enzyme family protein[more]
AT3G15355.17.8e-10246.12 ubiquitin-conjugating enzyme 25[more]
AT1G53023.11.6e-8353.85 Ubiquitin-conjugating enzyme family protein[more]
AT2G33770.11.7e-8052.35 phosphate 2[more]
AT2G16920.16.4e-8047.84 ubiquitin-conjugating enzyme 23[more]
Match NameE-valueIdentityDescription
gi|449447213|ref|XP_004141363.1|0.0e+0099.85PREDICTED: probable ubiquitin-conjugating enzyme E2 25 isoform X2 [Cucumis sativ... [more]
gi|659103082|ref|XP_008452464.1|0.0e+0096.91PREDICTED: probable ubiquitin-conjugating enzyme E2 25 isoform X3 [Cucumis melo][more]
gi|778695935|ref|XP_011654074.1|0.0e+0089.84PREDICTED: probable ubiquitin-conjugating enzyme E2 25 isoform X1 [Cucumis sativ... [more]
gi|659103080|ref|XP_008452463.1|0.0e+0087.54PREDICTED: probable ubiquitin-conjugating enzyme E2 25 isoform X2 [Cucumis melo][more]
gi|659103078|ref|XP_008452462.1|0.0e+0087.54PREDICTED: probable ubiquitin-conjugating enzyme E2 25 isoform X1 [Cucumis melo][more]
The following terms have been associated with this gene:
Vocabulary: INTERPRO
TermDefinition
IPR000608UBQ-conjugat_E2
IPR016135UBQ-conjugating_enzyme/RWD
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0008150 biological_process
biological_process GO:0009987 cellular process
biological_process GO:0044699 single-organism process
cellular_component GO:0005575 cellular_component
molecular_function GO:0003674 molecular_function

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Cucsa.123170.1Cucsa.123170.1mRNA


Analysis Name: InterPro Annotations of cucumber (Gy14)
Date Performed: 2017-01-17
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR000608Ubiquitin-conjugating enzyme E2PFAMPF00179UQ_concoord: 383..527
score: 5.8
IPR000608Ubiquitin-conjugating enzyme E2PROFILEPS50127UBIQUITIN_CONJUGAT_2coord: 381..502
score: 19
IPR016135Ubiquitin-conjugating enzyme/RWD-likeGENE3DG3DSA:3.10.110.10coord: 350..616
score: 7.4
IPR016135Ubiquitin-conjugating enzyme/RWD-likeunknownSSF54495UBC-likecoord: 363..546
score: 2.08
NoneNo IPR availablePANTHERPTHR24067UBIQUITIN-CONJUGATING ENZYME E2coord: 361..629
score: 5.8E
NoneNo IPR availablePANTHERPTHR24067:SF100UBIQUITIN-CONJUGATING ENZYME FAMILY PROTEIN-RELATEDcoord: 361..629
score: 5.8E
NoneNo IPR availableSMARTSM00212ubc_7coord: 381..536
score: 4.7

The following gene(s) are orthologous to this gene:
GeneOrthologueOrganismBlock
Cucsa.123170Cla009619Watermelon (97103) v1cgywmB197
Cucsa.123170Cla023386Watermelon (97103) v1cgywmB198
Cucsa.123170Csa6G517350Cucumber (Chinese Long) v2cgycuB164
Cucsa.123170Csa4G637700Cucumber (Chinese Long) v2cgycuB163
Cucsa.123170MELO3C016701Melon (DHL92) v3.5.1cgymeB189
Cucsa.123170ClCG11G018090Watermelon (Charleston Gray)cgywcgB192
Cucsa.123170ClCG01G022980Watermelon (Charleston Gray)cgywcgB193
Cucsa.123170CSPI06G34610Wild cucumber (PI 183967)cgycpiB165
Cucsa.123170CSPI04G23510Wild cucumber (PI 183967)cgycpiB164
Cucsa.123170CmaCh09G008670Cucurbita maxima (Rimu)cgycmaB0319
Cucsa.123170CmaCh01G012070Cucurbita maxima (Rimu)cgycmaB0322
Cucsa.123170CmoCh09G008560Cucurbita moschata (Rifu)cgycmoB0316
Cucsa.123170CmoCh01G012600Cucurbita moschata (Rifu)cgycmoB0318
Cucsa.123170Lsi01G003180Bottle gourd (USVL1VR-Ls)cgylsiB183
Cucsa.123170Cp4.1LG02g01690Cucurbita pepo (Zucchini)cgycpeB0310
Cucsa.123170Cp4.1LG06g06900Cucurbita pepo (Zucchini)cgycpeB0312
Cucsa.123170MELO3C016701.2Melon (DHL92) v3.6.1cgymedB187
Cucsa.123170MELO3C007283.2Melon (DHL92) v3.6.1cgymedB188
Cucsa.123170CsaV3_4G033720Cucumber (Chinese Long) v3cgycucB167
Cucsa.123170CsaV3_6G050140Cucumber (Chinese Long) v3cgycucB168
Cucsa.123170Cla97C01G022870Watermelon (97103) v2cgywmbB188
Cucsa.123170Cla97C11G224160Watermelon (97103) v2cgywmbB189
Cucsa.123170Bhi09G002799Wax gourdcgywgoB223
Cucsa.123170Bhi03G000319Wax gourdcgywgoB222
Cucsa.123170Carg17102Silver-seed gourdcarcgyB0208
Cucsa.123170Carg14696Silver-seed gourdcarcgyB0365
The following gene(s) are paralogous to this gene:
GeneParalogueOrganismBlock
Cucsa.123170Cucsa.046210Cucumber (Gy14) v1cgycgyB003