Cucsa.121840 (gene) Cucumber (Gy14) v1

BLAST of Cucsa.121840 vs. Swiss-Prot
Match: LAC1_ARATH (Laccase-1 OS=Arabidopsis thaliana GN=LAC1 PE=2 SV=1)

HSP 1 Score: 817.8 bits (2111), Expect = 7.8e-236
Identity = 392/582 (67.35%), Postives = 461/582 (79.21%), Query Frame = 1

Query: 12  LLVIFISILLAGFVPFSFASPVIRRFQFNVEWKKVTRLCHTKQLLTVNGQYPGPTIAVHE 71
           L++    +L    +P+S AS   RRF FNVEWKKVTRLCHTKQLLTVNGQYPGPT+AVHE
Sbjct: 7   LIISTFLLLFTTLLPYSSASTT-RRFHFNVEWKKVTRLCHTKQLLTVNGQYPGPTVAVHE 66

Query: 72  GDTVEIKVNNCINENTTIHWHGVKQLRTGWADGPAYITQCPIRTGESYTYKFSVIDQRGT 131
           GD VEIKV N I  NTTIHWHG++Q RTGWADGPAYITQCPIR+ +SYTY+F V DQRGT
Sbjct: 67  GDIVEIKVTNRIAHNTTIHWHGLRQYRTGWADGPAYITQCPIRSKQSYTYRFKVEDQRGT 126

Query: 132 LWWHAHYSWQRASVHGAFIIYPRMPYPFSTFPIEAGIPLIFGEWWNGDVEEVENEMLRSG 191
           L WHAH+SWQRASV+GAFIIYPR PYPFS   I++ IP+I GEWWN DV+ VE  M+++G
Sbjct: 127 LLWHAHHSWQRASVYGAFIIYPRQPYPFSGSHIQSEIPIILGEWWNDDVDNVEKAMMKTG 186

Query: 192 GGPNNSDAYTINGLPGPLYPCSSTDTFISTVERGKTYLLRVINGALNDELFFAIANHTLT 251
            G   SDAYT+NGLPGPLYPCS+ DTF +TV+ GKTY+LR+IN ALN+ELF A+ANHTLT
Sbjct: 187 AGAKVSDAYTLNGLPGPLYPCSTKDTFTATVDAGKTYILRIINAALNNELFVAVANHTLT 246

Query: 252 VVEIDAAYTKPFNTTAIMIAPGQTTTLLLNTDQIQIPDHSSEFLFPMAITPYVTSNFPFN 311
           VVE+DA YTKP +T AIMIAPGQTTTLLL  DQ+       EFL  +A TPYVTS FPFN
Sbjct: 247 VVEVDAVYTKPVHTKAIMIAPGQTTTLLLRADQLS----GGEFL--IAATPYVTSVFPFN 306

Query: 312 NSTSIGFLRY--KSRKMNKL--KRQKKFPSNQIPENLPDMKDTAFATAFSSKLRSLNSSL 371
           NST++GF+RY  K++  N +  +R+++  +      LP+M DT FAT FS  ++SL S+ 
Sbjct: 307 NSTTVGFIRYTGKTKPENSVNTRRRRRLTAMSTVVALPNMLDTKFATKFSDSIKSLGSAK 366

Query: 372 YPCNVPKTVHKRVFVTISLNLQNCPSGKTCKGLNGKRFFASMNNQSFIRPDSSILESHYR 431
           YPC VP  + KRV  TISLNLQ+CP  +TC G  GKRFFASMNN SF+RP  SILES+Y+
Sbjct: 367 YPCKVPTKIDKRVITTISLNLQDCPLNQTCDGYAGKRFFASMNNISFVRPPISILESYYK 426

Query: 432 KIDTNSYSTDFPEKPIRFFDYTGVNPLSRNMNTEFGTKLLAVPYGTNLEIVFQGTNFLSV 491
           K     +S DFPEKP   FD+TGV+P+S NMNTEFGTKL  V +G+ LEIVFQGT+FL++
Sbjct: 427 KQSKGVFSLDFPEKPPNRFDFTGVDPVSENMNTEFGTKLFEVEFGSRLEIVFQGTSFLNI 486

Query: 492 ENHPIHVHGHNFFVVGRGFGNFNVGKDPANYNLVDPPERNTVAVPRGGWAAIRIKADNPG 551
           ENHP+HVHGHNFFVVGRGFGNF+  KDP  YNLVDPPERNT AVP GGWAAIRI ADNPG
Sbjct: 487 ENHPLHVHGHNFFVVGRGFGNFDPEKDPKRYNLVDPPERNTFAVPTGGWAAIRINADNPG 546

Query: 552 VWFIHCHLEEHTSWGLAMGLIVRNGAGDSKSLLPPPDDLPLC 590
           VWFIHCHLE+HTSWGLAMG IV++G   S++LLPPP DLP C
Sbjct: 547 VWFIHCHLEQHTSWGLAMGFIVKDGPLPSQTLLPPPHDLPQC 581

BLAST of Cucsa.121840 vs. Swiss-Prot
Match: LAC4_ORYSJ (Laccase-4 OS=Oryza sativa subsp. japonica GN=LAC4 PE=2 SV=1)

HSP 1 Score: 622.5 bits (1604), Expect = 4.8e-177
Identity = 299/563 (53.11%), Postives = 388/563 (68.92%), Query Frame = 1

Query: 30  ASPVIRRFQFNVEWKKVTRLCHTKQLLTVNGQYPGPTIAVHEGDTVEIKVNNCINENTTI 89
           A  + R ++FNV+    TRLC+TK ++TVNGQ PGP +   EGD V I+V N +  N ++
Sbjct: 26  AQGITRHYEFNVQMANATRLCNTKSMVTVNGQCPGPELVAREGDRVVIRVTNNVAHNISL 85

Query: 90  HWHGVKQLRTGWADGPAYITQCPIRTGESYTYKFSVIDQRGTLWWHAHYSWQRASVHGAF 149
           HWHGV+Q+RTGWADGPAYITQCPI+TG+SY Y F+V  QRGTLWWHAH SW RA+V+GA 
Sbjct: 86  HWHGVRQVRTGWADGPAYITQCPIQTGQSYVYNFTVAGQRGTLWWHAHISWLRATVYGAL 145

Query: 150 IIYPRM--PYPFSTFPIEAGIPLIFGEWWNGDVEEVENEMLRSGGGPNNSDAYTINGLPG 209
           +I P++  PYPF     E  +P+IFGEWWN D EEV N+ +++GGGPN SDA+TINGLPG
Sbjct: 146 VILPKLGVPYPFPAPHKE--VPVIFGEWWNADTEEVVNQAVQTGGGPNVSDAFTINGLPG 205

Query: 210 PLYPCSSTDTFISTVERGKTYLLRVINGALNDELFFAIANHTLTVVEIDAAYTKPFNTTA 269
           PLY CS+ DTF   V+ GKTY+LR+IN ALN+ELFFA+ANHTLTVVE+DA Y KPF    
Sbjct: 206 PLYNCSAQDTFKLKVKPGKTYMLRLINAALNEELFFAVANHTLTVVEVDAVYVKPFTVDT 265

Query: 270 IMIAPGQTTTLLLNTDQIQIPDHSSEFLFPMAITPYVTSN-FPFNNSTSIGFLRYKSRKM 329
           ++I+PGQTT +LL T +   P  +    F M+  PY T+    F N+T  G L Y++  M
Sbjct: 266 LVISPGQTTNVLL-TAKPYYPGAN----FYMSAAPYSTARPGTFGNTTVAGILEYENPAM 325

Query: 330 NKLKRQKKFPSNQIPENLPDMKDTAFATAFSSKLRSLNSSLYPCNVPKTVHKRVFVTISL 389
           +                LP + DT F T F+ KLRSL +  YP  VP++V KR F T+ L
Sbjct: 326 SPSAASFVKGLPLFKPTLPQLNDTDFVTNFTDKLRSLATPEYPAAVPQSVDKRFFFTVGL 385

Query: 390 NLQNCPSGKTCKGLNGKRFFASMNNQSFIRPDSSILESHYRKIDTNSYSTDFPEKPIRFF 449
               CP+  TC+G N  +  ASMNN SF+ P  ++L+SH+  + +  Y+ DFP  P+  F
Sbjct: 386 GTLPCPANMTCQGPNNTQMAASMNNVSFVLPARALLQSHFTGLSSGVYAPDFPVAPLSPF 445

Query: 450 DYTGVNPLSRNMNTEFGTKLLAVPYGTNLEIVFQGTNFLSVENHPIHVHGHNFFVVGRGF 509
           +YTG  P   N N + GTKLL + Y T++E+V Q T+ L +E+HP+H+HG NFFV+G+GF
Sbjct: 446 NYTGTPP--NNTNVKTGTKLLVLRYNTSVELVMQDTSILGIESHPLHLHGFNFFVIGQGF 505

Query: 510 GNFNVGKDPANYNLVDPPERNTVAVPRGGWAAIRIKADNPGVWFIHCHLEEHTSWGLAMG 569
           GN++   DPA +NLVDP ERNTV VP GGW AIR  ADNPGVWF+HCHLE HT+WGL M 
Sbjct: 506 GNYDAVNDPAKFNLVDPVERNTVGVPAGGWVAIRFLADNPGVWFMHCHLEAHTTWGLRMA 565

Query: 570 LIVRNGAGDSKSLLPPPDDLPLC 590
            +V +G+  ++ LLPPP DLP C
Sbjct: 566 WLVLDGSHPNQKLLPPPSDLPKC 579

BLAST of Cucsa.121840 vs. Swiss-Prot
Match: LAC17_ARATH (Laccase-17 OS=Arabidopsis thaliana GN=LAC17 PE=2 SV=1)

HSP 1 Score: 610.5 bits (1573), Expect = 1.9e-173
Identity = 310/586 (52.90%), Postives = 390/586 (66.55%), Query Frame = 1

Query: 11  MLLVIFISILLAGFVPFSFASPVIRRFQFNVEWKKVTRLCHTKQLLTVNGQYPGPTIAVH 70
           +LL +F  +LL     F     + R +   ++ + VTRLCHTK L++VNGQ+PGP +   
Sbjct: 5   LLLAVFSCVLLLPQPAFG----ITRHYTLEIKMQNVTRLCHTKSLVSVNGQFPGPKLIAR 64

Query: 71  EGDTVEIKVNNCINENTTIHWHGVKQLRTGWADGPAYITQCPIRTGESYTYKFSVIDQRG 130
           EGD V IKV N +  N ++HWHG++QLR+GWADGPAYITQCPI+TG+SY Y ++++ QRG
Sbjct: 65  EGDQVLIKVVNQVPNNISLHWHGIRQLRSGWADGPAYITQCPIQTGQSYVYNYTIVGQRG 124

Query: 131 TLWWHAHYSWQRASVHGAFIIYPR--MPYPFSTFPIEAGIPLIFGEWWNGDVEEVENEML 190
           TLW+HAH SW R++V+G  II P+  +PYPF+    E  +P+IFGEW+N D E +  +  
Sbjct: 125 TLWYHAHISWLRSTVYGPLIILPKRGVPYPFAKPHKE--VPMIFGEWFNADTEAIIRQAT 184

Query: 191 RSGGGPNNSDAYTINGLPGPLYPCSSTDTFISTVERGKTYLLRVINGALNDELFFAIANH 250
           ++GGGPN SDAYTINGLPGPLY CS+ DTF   V+ GKTYLLR+IN ALNDELFF+IANH
Sbjct: 185 QTGGGPNVSDAYTINGLPGPLYNCSAKDTFRLRVKPGKTYLLRLINAALNDELFFSIANH 244

Query: 251 TLTVVEIDAAYTKPFNTTAIMIAPGQTTTLLLNTDQIQIPDHSSEFLFPMAITPYVTSNF 310
           T+TVVE DA Y KPF T  I+IAPGQTT +LL T +   P  S    F M   PYVT   
Sbjct: 245 TVTVVEADAIYVKPFETETILIAPGQTTNVLLKT-KSSYPSAS----FFMTARPYVTGQG 304

Query: 311 PFNNSTSIGFLRYKSRKMNKLKRQKKFPSN--QIPENLPDMKDTAFATAFSSKLRSLNSS 370
            F+NST  G L Y+  K  K    +    N       LP + DT FAT FS+KLRSLNS 
Sbjct: 305 TFDNSTVAGILEYEPPKQTKGAHSRTSIKNLQLFKPILPALNDTNFATKFSNKLRSLNSK 364

Query: 371 LYPCNVPKTVHKRVFVTISLNLQNC--PSGKTCKG-LNGKRFFASMNNQSFIRPDSSILE 430
            +P NVP  V ++ F T+ L    C   + +TC+G  N   F AS++N SF  P  ++L+
Sbjct: 365 NFPANVPLNVDRKFFFTVGLGTNPCNHKNNQTCQGPTNTTMFAASISNISFTMPTKALLQ 424

Query: 431 SHYRKIDTNSYSTDFPEKPIRFFDYTGVNPLSRNMNTEFGTKLLAVPYGTNLEIVFQGTN 490
           SHY       YS  FP  PI  F+YTG  P   N     GT L+ +PY T++E+V Q T+
Sbjct: 425 SHYSGQSHGVYSPKFPWSPIVPFNYTGTPP--NNTMVSNGTNLMVLPYNTSVELVMQDTS 484

Query: 491 FLSVENHPIHVHGHNFFVVGRGFGNFNVGKDPANYNLVDPPERNTVAVPRGGWAAIRIKA 550
            L  E+HP+H+HG NFFVVG+GFGNF+  KDP N+NLVDP ERNTV VP GGWAAIR  A
Sbjct: 485 ILGAESHPLHLHGFNFFVVGQGFGNFDPNKDPRNFNLVDPIERNTVGVPSGGWAAIRFLA 544

Query: 551 DNPGVWFIHCHLEEHTSWGLAMGLIVRNGAGDSKSLLPPPDDLPLC 590
           DNPGVWF+HCHLE HTSWGL M  +V +G    + LLPPP DLP C
Sbjct: 545 DNPGVWFMHCHLEVHTSWGLRMAWLVLDGDKPDQKLLPPPADLPKC 577

BLAST of Cucsa.121840 vs. Swiss-Prot
Match: LAC11_ARATH (Laccase-11 OS=Arabidopsis thaliana GN=LAC11 PE=2 SV=1)

HSP 1 Score: 597.0 bits (1538), Expect = 2.2e-169
Identity = 297/584 (50.86%), Postives = 386/584 (66.10%), Query Frame = 1

Query: 10  MMLLVIFISILLAGFVPFSFASPVIRRFQFNVEWKKVTRLCHTKQLLTVNGQYPGPTIAV 69
           M +  +F+   L  F+ +S     ++++QF+V+ K ++R+C+ K ++TVNG +PGPT+  
Sbjct: 1   MKMGFLFLFCYLLAFLGYSPVDAAVKKYQFDVQVKNISRICNAKPIVTVNGMFPGPTVYA 60

Query: 70  HEGDTVEIKVNNCINENTTIHWHGVKQLRTGWADGPAYITQCPIRTGESYTYKFSVIDQR 129
            EGD V I V N +  N +IHWHG+KQ R GWADGPAYITQCPI+TG+SY Y F+V  QR
Sbjct: 61  REGDRVIINVTNHVQYNMSIHWHGLKQYRNGWADGPAYITQCPIQTGQSYLYDFNVTGQR 120

Query: 130 GTLWWHAHYSWQRASVHGAFIIYPR--MPYPFSTFPIEAGIPLIFGEWWNGDVEEVENEM 189
           GTLWWHAH  W RA+V+GA +I P    PYPF     E+ I  I GEWWN DVE   N+ 
Sbjct: 121 GTLWWHAHILWLRATVYGAIVILPAPGKPYPFPQPYQESNI--ILGEWWNKDVETAVNQA 180

Query: 190 LRSGGGPNNSDAYTINGLPGPLYPCSSTDTFISTVERGKTYLLRVINGALNDELFFAIAN 249
            + G  P  SDA+TING PGPL+PCS   TF+   E GKTYLLR+IN ALNDELFF IA 
Sbjct: 181 NQLGAPPPMSDAHTINGKPGPLFPCSEKHTFVIEAEAGKTYLLRIINAALNDELFFGIAG 240

Query: 250 HTLTVVEIDAAYTKPFNTTAIMIAPGQTTTLLLNTDQIQIPDHSSEFLFPMAITPYVTSN 309
           H +TVVEIDA YTKPF T AI++ PGQTT +L+ TD+       S   + MA +P++ + 
Sbjct: 241 HNMTVVEIDAVYTKPFTTKAILLGPGQTTNVLVKTDR-------SPNRYFMAASPFMDAP 300

Query: 310 FPFNNSTSIGFLRYKSRKMNKLKRQKKFPSNQIP--ENLPDMKDTAFATAFSSKLRSLNS 369
              +N T    L+YK             P+  +P    LP   DT+FA  ++ KL+SLN+
Sbjct: 301 VSVDNKTVTAILQYKG-----------VPNTVLPILPKLPLPNDTSFALDYNGKLKSLNT 360

Query: 370 SLYPCNVPKTVHKRVFVTISLNLQNCPSGKTCKGLNGKRFFASMNNQSFIRPDSSILESH 429
             +P  VP  V +R+F TI L +  CP   TC  +NG    AS+NN +FI P +++L++H
Sbjct: 361 PNFPALVPLKVDRRLFYTIGLGINACP---TC--VNGTNLAASINNITFIMPKTALLKAH 420

Query: 430 YRKIDTNSYSTDFPEKPIRFFDYTGVNPLSRNMNTEFGTKLLAVPYGTNLEIVFQGTNFL 489
           Y  I +  + TDFP++P + F+YTGV PL+ N+ T  GT+L  V + T +E+V Q TN L
Sbjct: 421 YSNI-SGVFRTDFPDRPPKAFNYTGV-PLTANLGTSTGTRLSRVKFNTTIELVLQDTNLL 480

Query: 490 SVENHPIHVHGHNFFVVGRGFGNFNVGKDPANYNLVDPPERNTVAVPRGGWAAIRIKADN 549
           +VE+HP H+HG+NFFVVG G GNF+  KDPA +NLVDPPERNTV VP GGWAAIR +ADN
Sbjct: 481 TVESHPFHLHGYNFFVVGTGVGNFDPKKDPAKFNLVDPPERNTVGVPTGGWAAIRFRADN 540

Query: 550 PGVWFIHCHLEEHTSWGLAMGLIVRNGAGDSKSLLPPPDDLPLC 590
           PGVWF+HCHLE HT WGL M  +V NG     S+LPPP D P C
Sbjct: 541 PGVWFMHCHLEVHTMWGLKMAFVVENGETPELSVLPPPKDYPSC 557

BLAST of Cucsa.121840 vs. Swiss-Prot
Match: LAC12_ORYSJ (Laccase-12 OS=Oryza sativa subsp. japonica GN=LAC12 PE=3 SV=2)

HSP 1 Score: 592.0 bits (1525), Expect = 7.0e-168
Identity = 303/591 (51.27%), Postives = 386/591 (65.31%), Query Frame = 1

Query: 1   MKSLSQQCRMMLLVIFISILLAGFVPFSFASPVIRRFQFNVEWKKVTRLCHTKQLLTVNG 60
           M + S   R  LLV  +  L A       A  + R++ F+V+   VTRLC TK ++TVNG
Sbjct: 1   MAAASSVLRCCLLVAALMTLSA-----MGAEAITRQYLFDVQTTSVTRLCSTKSIVTVNG 60

Query: 61  QYPGPTIAVHEGDTVEIKVNNCINENTTIHWHGVKQLRTGWADGPAYITQCPIRTGESYT 120
           QYPGPT+   EGD VE+ V N    N +IHWHG++QL +GWADGP+YITQCPI+ G SY 
Sbjct: 61  QYPGPTLFAREGDHVEVTVVNHSPYNMSIHWHGIRQLLSGWADGPSYITQCPIQPGGSYV 120

Query: 121 YKFSVIDQRGTLWWHAHYSWQRASVHGAFIIYP--RMPYPFSTFPIEAGIPLIFGEWWNG 180
           Y+F++  QRGTLWWHAH SW RA+VHG  +I P   + YPF   P E  +P++FGEWWN 
Sbjct: 121 YRFTITGQRGTLWWHAHISWLRATVHGPMVILPPAGVGYPFPA-PHEE-VPIMFGEWWNN 180

Query: 181 DVEEVENEMLRSGGGPNNSDAYTINGLPGPLYPCSSTDTFISTVERGKTYLLRVINGALN 240
           D E V ++ L++GGGPN SDAYT+NGLPGPLY CS+ DTF   V+ GKTY+LR+IN ALN
Sbjct: 181 DTEAVISQALQTGGGPNISDAYTLNGLPGPLYNCSAQDTFKLKVKPGKTYMLRLINAALN 240

Query: 241 DELFFAIANHTLTVVEIDAAYTKPFNTTAIMIAPGQTTTLLLNTDQIQIPDHSSEFLFPM 300
           DELFF+IANHTLTVV++DA Y KPF    ++IAPGQT+ +LL T +   P  S    + M
Sbjct: 241 DELFFSIANHTLTVVDVDALYVKPFTVDTLIIAPGQTSNVLL-TAKPTYPGAS----YYM 300

Query: 301 AITPYVTSNFPFNNSTSIGFLRYKSRKMNKLKRQKKFPSNQIPENLPDMKDTAFATAFSS 360
              PY T+   F+N+T  G L Y           K  P       LP + DT   + F++
Sbjct: 301 LARPYTTTQGTFDNTTVAGVLEYDD-PCPTTAAGKIVPI--FSPTLPQINDTNAVSNFTA 360

Query: 361 KLRSLNSSLYPCNVPKTVHKRVFVTISLNLQNCPSGKTCKGLNGKRFFASMNNQSFIRPD 420
           KLRSL S+ YP  VP+ V  R F T+ L    C    TC+G NG RF AS+NN SF+ P 
Sbjct: 361 KLRSLASAGYPAAVPQQVDHRFFFTVGLGTHPCAVNGTCQGPNGSRFAASINNVSFVLPA 420

Query: 421 SSILESHYRKIDTNSYSTDFPEKPIRFFDYTGVNPLSRNMNTEFGTKLLAVPYGTNLEIV 480
           +++L+SH+       Y+++FP  P+  F+YTG  P   N N   GTK+L +PYG N+E+V
Sbjct: 421 TALLQSHFAGKSKGVYASNFPYYPLNPFNYTGTPP--NNTNVMNGTKVLVLPYGANVELV 480

Query: 481 FQGTNFLSVENHPIHVHGHNFFVVGRGFGNFNVGKDPANYNLVDPPERNTVAVPRGGWAA 540
            Q T+ L  E+HP+H+HG NFFVVG+GFGNF+   DPA +NL DP ERNTV VP GGW A
Sbjct: 481 MQDTSILGAESHPLHLHGFNFFVVGQGFGNFDPINDPAKFNLYDPVERNTVGVPAGGWVA 540

Query: 541 IRIKADNPGVWFIHCHLEEHTSWGLAMGLIVRNGAGDSKSLLPPPDDLPLC 590
           IR  ADNPGVWF+HCHLE H SWGL M  +V +G+   + L PPP DLP C
Sbjct: 541 IRFHADNPGVWFMHCHLEVHMSWGLKMAWLVLDGSRPDQKLPPPPLDLPKC 574

BLAST of Cucsa.121840 vs. TrEMBL
Match: A0A0A0LQV8_CUCSA (Laccase OS=Cucumis sativus GN=Csa_1G042710 PE=3 SV=1)

HSP 1 Score: 1031.6 bits (2666), Expect = 3.8e-298
Identity = 493/494 (99.80%), Postives = 493/494 (99.80%), Query Frame = 1

Query: 96  QLRTGWADGPAYITQCPIRTGESYTYKFSVIDQRGTLWWHAHYSWQRASVHGAFIIYPRM 155
           QLRTGWADGPAYITQCPIRTGESYTYKFSVIDQRGTLWWHAHYSWQRASVHGAFIIYPRM
Sbjct: 41  QLRTGWADGPAYITQCPIRTGESYTYKFSVIDQRGTLWWHAHYSWQRASVHGAFIIYPRM 100

Query: 156 PYPFSTFPIEAGIPLIFGEWWNGDVEEVENEMLRSGGGPNNSDAYTINGLPGPLYPCSST 215
           PYPFSTFPIEAGIPLIFGEWWNGDVEEVENEMLRSGGGPNNSDAYTINGLPGPLYPCSST
Sbjct: 101 PYPFSTFPIEAGIPLIFGEWWNGDVEEVENEMLRSGGGPNNSDAYTINGLPGPLYPCSST 160

Query: 216 DTFISTVERGKTYLLRVINGALNDELFFAIANHTLTVVEIDAAYTKPFNTTAIMIAPGQT 275
           DTFISTVERGKTYLLRVINGALNDELFFAIANHTLTVVEIDAAYTKPFNTTAIMIAPGQT
Sbjct: 161 DTFISTVERGKTYLLRVINGALNDELFFAIANHTLTVVEIDAAYTKPFNTTAIMIAPGQT 220

Query: 276 TTLLLNTDQIQIPDHSSEFLFPMAITPYVTSNFPFNNSTSIGFLRYKSRKMNKLKRQKKF 335
           TTLLLNTDQIQIPDHSSEFLFPMAITPYVTSNFPFNNSTSIGFLRYKSRKMNKLKRQKKF
Sbjct: 221 TTLLLNTDQIQIPDHSSEFLFPMAITPYVTSNFPFNNSTSIGFLRYKSRKMNKLKRQKKF 280

Query: 336 PSNQIPENLPDMKDTAFATAFSSKLRSLNSSLYPCNVPKTVHKRVFVTISLNLQNCPSGK 395
           PSNQIPENLPDMKDTAFATAFSSKLRSLNSSLYPCNVPKTVHKRVFVTISLNLQNCPSGK
Sbjct: 281 PSNQIPENLPDMKDTAFATAFSSKLRSLNSSLYPCNVPKTVHKRVFVTISLNLQNCPSGK 340

Query: 396 TCKGLNGKRFFASMNNQSFIRPDSSILESHYRKIDTNSYSTDFPEKPIRFFDYTGVNPLS 455
           TCKGLNGKRFFASMNNQSFIRPDSSILESHYRKIDTNSYSTDFPEKPIRFF YTGVNPLS
Sbjct: 341 TCKGLNGKRFFASMNNQSFIRPDSSILESHYRKIDTNSYSTDFPEKPIRFFYYTGVNPLS 400

Query: 456 RNMNTEFGTKLLAVPYGTNLEIVFQGTNFLSVENHPIHVHGHNFFVVGRGFGNFNVGKDP 515
           RNMNTEFGTKLLAVPYGTNLEIVFQGTNFLSVENHPIHVHGHNFFVVGRGFGNFNVGKDP
Sbjct: 401 RNMNTEFGTKLLAVPYGTNLEIVFQGTNFLSVENHPIHVHGHNFFVVGRGFGNFNVGKDP 460

Query: 516 ANYNLVDPPERNTVAVPRGGWAAIRIKADNPGVWFIHCHLEEHTSWGLAMGLIVRNGAGD 575
           ANYNLVDPPERNTVAVPRGGWAAIRIKADNPGVWFIHCHLEEHTSWGLAMGLIVRNGAGD
Sbjct: 461 ANYNLVDPPERNTVAVPRGGWAAIRIKADNPGVWFIHCHLEEHTSWGLAMGLIVRNGAGD 520

Query: 576 SKSLLPPPDDLPLC 590
           SKSLLPPPDDLPLC
Sbjct: 521 SKSLLPPPDDLPLC 534

BLAST of Cucsa.121840 vs. TrEMBL
Match: A0A0A0LQV8_CUCSA (Laccase OS=Cucumis sativus GN=Csa_1G042710 PE=3 SV=1)

HSP 1 Score: 80.9 bits (198), Expect = 5.8e-12
Identity = 52/96 (54.17%), Postives = 59/96 (61.46%), Query Frame = 1

Query: 1  MKSLSQQCRMMLLVIFISILLAGFVPFSFASPVIRRFQFNVEWKKVTRLCHTKQLLTVNG 60
          MKSLSQQCRMMLLVIFISILLAGFVPFSFASPVIRRFQFN             QL T  G
Sbjct: 1  MKSLSQQCRMMLLVIFISILLAGFVPFSFASPVIRRFQFN-------------QLRT--G 60

Query: 61 QYPGPT----IAVHEGDTVEIKVNNCINENTTIHWH 93
             GP       +  G++   K  + I++  T+ WH
Sbjct: 61 WADGPAYITQCPIRTGESYTYKF-SVIDQRGTLWWH 80

HSP 2 Score: 882.1 bits (2278), Expect = 3.8e-253
Identity = 421/579 (72.71%), Postives = 473/579 (81.69%), Query Frame = 1

Query: 13  LVIFISILLAGFVPFSFASPVIRRFQFNVEWKKVTRLCHTKQLLTVNGQYPGPTIAVHEG 72
           L + + ++LAG +P  F S   R FQFNVEWK VTRLC TK LLTVNG+YPGPTIAVHEG
Sbjct: 10  LFVVMLMILAGALPSGF-SQTTRHFQFNVEWKNVTRLCSTKPLLTVNGEYPGPTIAVHEG 69

Query: 73  DTVEIKVNNCINENTTIHWHGVKQLRTGWADGPAYITQCPIRTGESYTYKFSVIDQRGTL 132
           D VEIKV N I +NTTIHWHG++QLRTGWADGPAYITQCPIR G+SYTYKF+V  QRGTL
Sbjct: 70  DHVEIKVTNHIADNTTIHWHGIRQLRTGWADGPAYITQCPIRGGKSYTYKFTVQYQRGTL 129

Query: 133 WWHAHYSWQRASVHGAFIIYPRMPYPFSTFPIEAGIPLIFGEWWNGDVEEVENEMLRSGG 192
           WWHAHY+WQRASV+GAFII+PRMP+PFS  PI+   P+IFGEWW GDV+EVENEM++ GG
Sbjct: 130 WWHAHYAWQRASVYGAFIIHPRMPFPFSA-PIQDEFPIIFGEWWIGDVDEVENEMVKYGG 189

Query: 193 GPNNSDAYTINGLPGPLYPCSSTDTFISTVERGKTYLLRVINGALNDELFFAIANHTLTV 252
           GPN SDAYTINGLPGP YPCS+ DTFI TVE GKTYLLR+IN ALNDELFFA+ANHTLTV
Sbjct: 190 GPNISDAYTINGLPGPFYPCSNKDTFIKTVEHGKTYLLRIINAALNDELFFAVANHTLTV 249

Query: 253 VEIDAAYTKPFNTTAIMIAPGQTTTLLLNTDQIQIPDHSSEFLFPMAITPYVTSNFPFNN 312
           VEIDA YTKPF  TAIM+APGQTT +LL  +Q+  PD S  FL  MA  PY+TS FPF+N
Sbjct: 250 VEIDAVYTKPFTATAIMVAPGQTTNVLLTANQV--PDSSGTFL--MAAWPYLTSVFPFDN 309

Query: 313 STSIGFLRYKSRKMNKLKRQKKFPSNQIPE--NLPDMKDTAFATAFSSKLRSLNSSLYPC 372
           ST+ GFLRYK+     +K  +K PS   P   NLP M+DT F T FS KLRSL S  YPC
Sbjct: 310 STTAGFLRYKNTVTENIKHPQKMPSTFEPNKYNLPKMEDTMFVTKFSDKLRSLASPQYPC 369

Query: 373 NVPKTVHKRVFVTISLNLQNCPSGKTCKGLNGKRFFASMNNQSFIRPDSSILESHYRKID 432
            VPKT+ KRV   +SLNLQ+CP+ KTCKG   KRFFASMNNQSF+RP  SILESHY K+ 
Sbjct: 370 TVPKTIDKRVVTVVSLNLQDCPANKTCKGYEDKRFFASMNNQSFVRPSLSILESHYNKLR 429

Query: 433 TNSYSTDFPEKPIRFFDYTGVNPLSRNMNTEFGTKLLAVPYGTNLEIVFQGTNFLSVENH 492
            N YSTDFPEKP++ FDYTGV+P S NMNT+F TK+L VPYGTNLEIV QGT+FL+VENH
Sbjct: 430 NNEYSTDFPEKPLKPFDYTGVDPASENMNTQFSTKILEVPYGTNLEIVLQGTSFLNVENH 489

Query: 493 PIHVHGHNFFVVGRGFGNFNVGKDPANYNLVDPPERNTVAVPRGGWAAIRIKADNPGVWF 552
           PIHVHGHNFF+VGRGFGNFNV KDP  YNL+DPPERNTVAVP GGWAAIR KADNPGVWF
Sbjct: 490 PIHVHGHNFFIVGRGFGNFNVAKDPTKYNLIDPPERNTVAVPTGGWAAIRFKADNPGVWF 549

Query: 553 IHCHLEEHTSWGLAMGLIVRNGAGDSKSLLPPPDDLPLC 590
           IHCHLEEHTSWGLA GLIV+NG   S+ +LPPP DLP C
Sbjct: 550 IHCHLEEHTSWGLASGLIVQNGPRASQRVLPPPKDLPSC 582

BLAST of Cucsa.121840 vs. TrEMBL
Match: F6GSK0_VITVI (Laccase OS=Vitis vinifera GN=VIT_17s0000g02590 PE=3 SV=1)

HSP 1 Score: 879.4 bits (2271), Expect = 2.4e-252
Identity = 419/583 (71.87%), Postives = 479/583 (82.16%), Query Frame = 1

Query: 10  MMLLVIFISILLAGFVPFSFASPVIRRFQFNVEWKKVTRLCHTKQLLTVNGQYPGPTIAV 69
           +M+ ++F++++       S +SP  RRFQFNV+WKKVTRLCHTK LLTVNG+YPGPTIAV
Sbjct: 12  LMMGILFLAVIFP-----SSSSPTTRRFQFNVQWKKVTRLCHTKPLLTVNGKYPGPTIAV 71

Query: 70  HEGDTVEIKVNNCINENTTIHWHGVKQLRTGWADGPAYITQCPIRTGESYTYKFSVIDQR 129
           HEGD VE+KV N I  NTTIHWHG++QLRTGWADGPAY+TQCPIR G+SYTYKFSV+D R
Sbjct: 72  HEGDNVEVKVTNLIATNTTIHWHGIRQLRTGWADGPAYVTQCPIRGGQSYTYKFSVVDHR 131

Query: 130 GTLWWHAHYSWQRASVHGAFIIYPRMPYPFSTFPIEAGIPLIFGEWWNGDVEEVENEMLR 189
           GTL WHAHYSWQR SV+GAFIIYPRMPYPF   PI+  +P+IFGEWWN D EEVE EM+R
Sbjct: 132 GTLLWHAHYSWQRVSVYGAFIIYPRMPYPFKA-PIQDEVPIIFGEWWNDDAEEVEREMMR 191

Query: 190 SGGGPNNSDAYTINGLPGPLYPCSSTDTFISTVERGKTYLLRVINGALNDELFFAIANHT 249
           +G GP  S+AYTING+PGPLYPCS+ DTFI  V  GKTYLLR+IN ALN ELFFAIANHT
Sbjct: 192 TGDGPKISEAYTINGMPGPLYPCSNKDTFIQNVVPGKTYLLRIINAALNAELFFAIANHT 251

Query: 250 LTVVEIDAAYTKPFNTTAIMIAPGQTTTLLLNTDQIQIPDHSSEFLFPMAITPYVTSNFP 309
           LTVVEID  YTKPF TTAIMIAPGQTT +LL  DQ+  PD S+  +FPMA   YVTS FP
Sbjct: 252 LTVVEIDGLYTKPFTTTAIMIAPGQTTNVLLTADQV--PDSSA--MFPMAANNYVTSIFP 311

Query: 310 FNNSTSIGFLRYKS---RKMNKLKRQKKFPSNQIPENLPDMKDTAFATAFSSKLRSLNSS 369
           F+NST++GFLRYK+    +   L R   +  + +  NLP MKDTAFAT FSSKLRSL S+
Sbjct: 312 FDNSTTMGFLRYKTITNPRAKPLNRPAVYSLSSL-YNLPQMKDTAFATGFSSKLRSLASA 371

Query: 370 LYPCNVPKTVHKRVFVTISLNLQNCPSGKTCKGLNGKRFFASMNNQSFIRPDSSILESHY 429
            YPCNVPKT+ KR+  TISLNLQ+CP+ +TCKG NGKRFFASMNNQSFIRP  SILE HY
Sbjct: 372 QYPCNVPKTIDKRIVTTISLNLQDCPANQTCKGYNGKRFFASMNNQSFIRPFESILECHY 431

Query: 430 RKIDTNSYSTDFPEKPIRFFDYTGVNPLSRNMNTEFGTKLLAVPYGTNLEIVFQGTNFLS 489
           + +  + +S+DFPEKP   FDYTGVNP + NMNT+FGTKLL VPYG++LEIV QGT+FLS
Sbjct: 432 KNLTNHLFSSDFPEKPPTTFDYTGVNPFTSNMNTQFGTKLLVVPYGSSLEIVMQGTSFLS 491

Query: 490 VENHPIHVHGHNFFVVGRGFGNFNVGKDPANYNLVDPPERNTVAVPRGGWAAIRIKADNP 549
           VENHPIH HGHNFF+VGRGFGNF+V KDP NYNLVDPPERNTVAVP GGWAAIR KADNP
Sbjct: 492 VENHPIHFHGHNFFIVGRGFGNFDVNKDPQNYNLVDPPERNTVAVPTGGWAAIRFKADNP 551

Query: 550 GVWFIHCHLEEHTSWGLAMGLIVRNGAGDSKSLLPPPDDLPLC 590
           GVWFIHCHLEEHTSWGLAMGL+V+NG   S+ LLPPP DLP C
Sbjct: 552 GVWFIHCHLEEHTSWGLAMGLVVQNGPNPSQCLLPPPADLPSC 583

BLAST of Cucsa.121840 vs. TrEMBL
Match: U5FP71_POPTR (Laccase OS=Populus trichocarpa GN=POPTR_0015s04350g PE=3 SV=1)

HSP 1 Score: 860.9 bits (2223), Expect = 9.0e-247
Identity = 414/576 (71.88%), Postives = 475/576 (82.47%), Query Frame = 1

Query: 19  ILLAGFVPFSFASP-----VIRRFQFNVEWKKVTRLCHTKQLLTVNGQYPGPTIAVHEGD 78
           ILLA     + A P       RRFQFNVEWKKVTRLC TKQLL VNGQYPGPTIAVHEGD
Sbjct: 10  ILLASLAIIAAALPCCSSETTRRFQFNVEWKKVTRLCTTKQLLMVNGQYPGPTIAVHEGD 69

Query: 79  TVEIKVNNCINENTTIHWHGVKQLRTGWADGPAYITQCPIRTGESYTYKFSVIDQRGTLW 138
            VEIKV N I +NTT+HWHGV+QLRTGWADGPAYITQCPIR G+SYTYKF+V  QRGTL 
Sbjct: 70  NVEIKVKNRIAQNTTLHWHGVRQLRTGWADGPAYITQCPIRGGQSYTYKFTVTGQRGTLL 129

Query: 139 WHAHYSWQRASVHGAFIIYPRMPYPFSTFPIEAGIPLIFGEWWNGDVEEVENEMLRSGGG 198
           WHAHY+WQRASV+GA IIYPR+PYPFS  PI+A IP+IFGEWWNGD +E+E  ML +GGG
Sbjct: 130 WHAHYAWQRASVYGALIIYPRIPYPFS-HPIQAEIPIIFGEWWNGDPDEIEKTMLLTGGG 189

Query: 199 PNNSDAYTINGLPGPLYPCSSTDTFISTVERGKTYLLRVINGALNDELFFAIANHTLTVV 258
           P++S+AYTINGLPGPLYPCS+ DTFI TVE GKTYLLR+IN AL +ELFFAIA HTLTVV
Sbjct: 190 PDSSNAYTINGLPGPLYPCSNQDTFIQTVEYGKTYLLRIINAALTNELFFAIAKHTLTVV 249

Query: 259 EIDAAYTKPFNTTAIMIAPGQTTTLLLNTDQIQIPDHSSEFLFPMAITPYVTSNFPFNNS 318
           E+DA YTKPF TT+IMIAPGQTTT+L+  +Q+  PD +   +F MA  PY+TS FPFNNS
Sbjct: 250 EVDAVYTKPFATTSIMIAPGQTTTVLMTANQV--PDFTG--MFVMAARPYLTSVFPFNNS 309

Query: 319 TSIGFLRYKSRKMNKLKRQKKFPSNQIPENLPDMKDTAFATAFSSKLRSLNSSLYPCNVP 378
           T+IGFLRYK+ +  K K     PS+    NLP M+DTAFAT FS K++SL S  YPCNVP
Sbjct: 310 TTIGFLRYKNARTWKGKSPVD-PSSLRLHNLPAMEDTAFATKFSDKIKSLASPQYPCNVP 369

Query: 379 KTVHKRVFVTISLNLQNCPSGKTCKGLNGKRFFASMNNQSFIRPDSSILESHYRKIDTNS 438
           KT+ KRV  TISLNLQ+CP  KTC GL GK FFASMNNQSF+RP  SILES+Y+ + T+S
Sbjct: 370 KTIDKRVITTISLNLQDCPENKTCLGLKGKSFFASMNNQSFVRPSISILESYYKNLTTSS 429

Query: 439 YSTDFPEKPIRFFDYTGVNPLSRNMNTEFGTKLLAVPYGTNLEIVFQGTNFLSVENHPIH 498
           +S+DFP+KP   FDYTGV+PL++NMNT+FGTKLL +PYGTN+EIV Q T+FL++ENHPIH
Sbjct: 430 FSSDFPQKPPNNFDYTGVHPLTQNMNTKFGTKLLVLPYGTNIEIVLQDTSFLNLENHPIH 489

Query: 499 VHGHNFFVVGRGFGNFNVGKDPANYNLVDPPERNTVAVPRGGWAAIRIKADNPGVWFIHC 558
           VHGHNFF+VG GFGNFN  +DP  YNLVDPPERNTVAVP GGWAAIRIKADNPGVWF+HC
Sbjct: 490 VHGHNFFIVGSGFGNFNEARDPKRYNLVDPPERNTVAVPSGGWAAIRIKADNPGVWFVHC 549

Query: 559 HLEEHTSWGLAMGLIVRNGAGDSKSLLPPPDDLPLC 590
           HLE+HTSWGLA G IV+NG G S+SLLPPP DLP C
Sbjct: 550 HLEQHTSWGLATGFIVQNGQGPSQSLLPPPHDLPSC 579

BLAST of Cucsa.121840 vs. TrEMBL
Match: B9IEA5_POPTR (Laccase OS=Populus trichocarpa GN=POPTR_0015s04370g PE=3 SV=2)

HSP 1 Score: 853.6 bits (2204), Expect = 1.4e-244
Identity = 408/576 (70.83%), Postives = 472/576 (81.94%), Query Frame = 1

Query: 19  ILLAGFVPFSFASP-----VIRRFQFNVEWKKVTRLCHTKQLLTVNGQYPGPTIAVHEGD 78
           ILLA     + A P       RRFQFNVEWK+VTRLC TKQLL VNGQYPGPTIAVHEGD
Sbjct: 10  ILLASLAIIAAALPCCSSQTTRRFQFNVEWKQVTRLCTTKQLLMVNGQYPGPTIAVHEGD 69

Query: 79  TVEIKVNNCINENTTIHWHGVKQLRTGWADGPAYITQCPIRTGESYTYKFSVIDQRGTLW 138
            VEI V N I +NTT+HWHGV+QLRTGWADGPAY+TQCPIR G+SYTYKF+V  QRGTL 
Sbjct: 70  NVEINVKNQIAQNTTLHWHGVRQLRTGWADGPAYVTQCPIRGGQSYTYKFTVTGQRGTLL 129

Query: 139 WHAHYSWQRASVHGAFIIYPRMPYPFSTFPIEAGIPLIFGEWWNGDVEEVENEMLRSGGG 198
           WHAHY+WQRASV+GAFIIYPR+PYPFS  PI+A IP+IFGEWWNGD +EVEN M+ +G G
Sbjct: 130 WHAHYAWQRASVYGAFIIYPRIPYPFS-HPIQAEIPIIFGEWWNGDPDEVENRMMLTGAG 189

Query: 199 PNNSDAYTINGLPGPLYPCSSTDTFISTVERGKTYLLRVINGALNDELFFAIANHTLTVV 258
           P++S+AYTINGLPGPLYPCS+ DT+I TVE GKTY+LR+IN AL DELFFAIA HTLTVV
Sbjct: 190 PDSSNAYTINGLPGPLYPCSNQDTYIQTVEYGKTYMLRIINAALADELFFAIAKHTLTVV 249

Query: 259 EIDAAYTKPFNTTAIMIAPGQTTTLLLNTDQIQIPDHSSEFLFPMAITPYVTSNFPFNNS 318
           E+DA YTKPF TT+IMIAPGQTTT+L+  +Q+  PD +   +F MA  PY+TS FPFNNS
Sbjct: 250 EVDAVYTKPFATTSIMIAPGQTTTVLMTANQV--PDFTG--MFVMAARPYLTSVFPFNNS 309

Query: 319 TSIGFLRYKSRKMNKLKRQKKFPSNQIPENLPDMKDTAFATAFSSKLRSLNSSLYPCNVP 378
           T+IGFLRYK+ +  K K     PS+    NLP M+DTAFAT FS K++SL S  YPCNVP
Sbjct: 310 TTIGFLRYKNARTWKGKSPVD-PSSLKLHNLPAMEDTAFATKFSDKIKSLASPQYPCNVP 369

Query: 379 KTVHKRVFVTISLNLQNCPSGKTCKGLNGKRFFASMNNQSFIRPDSSILESHYRKIDTNS 438
           KT+ KRV  TISLN+Q+CP  KTC G  GK FFASMNNQSF+RP  SILES+Y+ + T S
Sbjct: 370 KTIDKRVITTISLNIQDCPENKTCSGYKGKSFFASMNNQSFVRPSISILESYYKNLTTGS 429

Query: 439 YSTDFPEKPIRFFDYTGVNPLSRNMNTEFGTKLLAVPYGTNLEIVFQGTNFLSVENHPIH 498
           +S+DFPEKP   FDYTG +PL++NMNT+FGTKL+ VPYGTN+EIV Q T+F+++ENHPIH
Sbjct: 430 FSSDFPEKPPNNFDYTGGDPLTQNMNTKFGTKLIVVPYGTNVEIVLQDTSFVNLENHPIH 489

Query: 499 VHGHNFFVVGRGFGNFNVGKDPANYNLVDPPERNTVAVPRGGWAAIRIKADNPGVWFIHC 558
           VHGHNFF+VG GFGNFN  +DP  YNLVDPPERNTVAVP GGWAAIRIKADNPGVWFIHC
Sbjct: 490 VHGHNFFIVGSGFGNFNEARDPKRYNLVDPPERNTVAVPSGGWAAIRIKADNPGVWFIHC 549

Query: 559 HLEEHTSWGLAMGLIVRNGAGDSKSLLPPPDDLPLC 590
           HLE+HTSWGLA G IV+NG G S+S+LPPP DLP C
Sbjct: 550 HLEQHTSWGLATGFIVQNGQGPSQSMLPPPQDLPSC 579

BLAST of Cucsa.121840 vs. TAIR10
Match: AT1G18140.1 (AT1G18140.1 laccase 1)

HSP 1 Score: 817.8 bits (2111), Expect = 4.4e-237
Identity = 392/582 (67.35%), Postives = 461/582 (79.21%), Query Frame = 1

Query: 12  LLVIFISILLAGFVPFSFASPVIRRFQFNVEWKKVTRLCHTKQLLTVNGQYPGPTIAVHE 71
           L++    +L    +P+S AS   RRF FNVEWKKVTRLCHTKQLLTVNGQYPGPT+AVHE
Sbjct: 7   LIISTFLLLFTTLLPYSSASTT-RRFHFNVEWKKVTRLCHTKQLLTVNGQYPGPTVAVHE 66

Query: 72  GDTVEIKVNNCINENTTIHWHGVKQLRTGWADGPAYITQCPIRTGESYTYKFSVIDQRGT 131
           GD VEIKV N I  NTTIHWHG++Q RTGWADGPAYITQCPIR+ +SYTY+F V DQRGT
Sbjct: 67  GDIVEIKVTNRIAHNTTIHWHGLRQYRTGWADGPAYITQCPIRSKQSYTYRFKVEDQRGT 126

Query: 132 LWWHAHYSWQRASVHGAFIIYPRMPYPFSTFPIEAGIPLIFGEWWNGDVEEVENEMLRSG 191
           L WHAH+SWQRASV+GAFIIYPR PYPFS   I++ IP+I GEWWN DV+ VE  M+++G
Sbjct: 127 LLWHAHHSWQRASVYGAFIIYPRQPYPFSGSHIQSEIPIILGEWWNDDVDNVEKAMMKTG 186

Query: 192 GGPNNSDAYTINGLPGPLYPCSSTDTFISTVERGKTYLLRVINGALNDELFFAIANHTLT 251
            G   SDAYT+NGLPGPLYPCS+ DTF +TV+ GKTY+LR+IN ALN+ELF A+ANHTLT
Sbjct: 187 AGAKVSDAYTLNGLPGPLYPCSTKDTFTATVDAGKTYILRIINAALNNELFVAVANHTLT 246

Query: 252 VVEIDAAYTKPFNTTAIMIAPGQTTTLLLNTDQIQIPDHSSEFLFPMAITPYVTSNFPFN 311
           VVE+DA YTKP +T AIMIAPGQTTTLLL  DQ+       EFL  +A TPYVTS FPFN
Sbjct: 247 VVEVDAVYTKPVHTKAIMIAPGQTTTLLLRADQLS----GGEFL--IAATPYVTSVFPFN 306

Query: 312 NSTSIGFLRY--KSRKMNKL--KRQKKFPSNQIPENLPDMKDTAFATAFSSKLRSLNSSL 371
           NST++GF+RY  K++  N +  +R+++  +      LP+M DT FAT FS  ++SL S+ 
Sbjct: 307 NSTTVGFIRYTGKTKPENSVNTRRRRRLTAMSTVVALPNMLDTKFATKFSDSIKSLGSAK 366

Query: 372 YPCNVPKTVHKRVFVTISLNLQNCPSGKTCKGLNGKRFFASMNNQSFIRPDSSILESHYR 431
           YPC VP  + KRV  TISLNLQ+CP  +TC G  GKRFFASMNN SF+RP  SILES+Y+
Sbjct: 367 YPCKVPTKIDKRVITTISLNLQDCPLNQTCDGYAGKRFFASMNNISFVRPPISILESYYK 426

Query: 432 KIDTNSYSTDFPEKPIRFFDYTGVNPLSRNMNTEFGTKLLAVPYGTNLEIVFQGTNFLSV 491
           K     +S DFPEKP   FD+TGV+P+S NMNTEFGTKL  V +G+ LEIVFQGT+FL++
Sbjct: 427 KQSKGVFSLDFPEKPPNRFDFTGVDPVSENMNTEFGTKLFEVEFGSRLEIVFQGTSFLNI 486

Query: 492 ENHPIHVHGHNFFVVGRGFGNFNVGKDPANYNLVDPPERNTVAVPRGGWAAIRIKADNPG 551
           ENHP+HVHGHNFFVVGRGFGNF+  KDP  YNLVDPPERNT AVP GGWAAIRI ADNPG
Sbjct: 487 ENHPLHVHGHNFFVVGRGFGNFDPEKDPKRYNLVDPPERNTFAVPTGGWAAIRINADNPG 546

Query: 552 VWFIHCHLEEHTSWGLAMGLIVRNGAGDSKSLLPPPDDLPLC 590
           VWFIHCHLE+HTSWGLAMG IV++G   S++LLPPP DLP C
Sbjct: 547 VWFIHCHLEQHTSWGLAMGFIVKDGPLPSQTLLPPPHDLPQC 581

BLAST of Cucsa.121840 vs. TAIR10
Match: AT5G60020.1 (AT5G60020.1 laccase 17)

HSP 1 Score: 610.5 bits (1573), Expect = 1.1e-174
Identity = 310/586 (52.90%), Postives = 390/586 (66.55%), Query Frame = 1

Query: 11  MLLVIFISILLAGFVPFSFASPVIRRFQFNVEWKKVTRLCHTKQLLTVNGQYPGPTIAVH 70
           +LL +F  +LL     F     + R +   ++ + VTRLCHTK L++VNGQ+PGP +   
Sbjct: 5   LLLAVFSCVLLLPQPAFG----ITRHYTLEIKMQNVTRLCHTKSLVSVNGQFPGPKLIAR 64

Query: 71  EGDTVEIKVNNCINENTTIHWHGVKQLRTGWADGPAYITQCPIRTGESYTYKFSVIDQRG 130
           EGD V IKV N +  N ++HWHG++QLR+GWADGPAYITQCPI+TG+SY Y ++++ QRG
Sbjct: 65  EGDQVLIKVVNQVPNNISLHWHGIRQLRSGWADGPAYITQCPIQTGQSYVYNYTIVGQRG 124

Query: 131 TLWWHAHYSWQRASVHGAFIIYPR--MPYPFSTFPIEAGIPLIFGEWWNGDVEEVENEML 190
           TLW+HAH SW R++V+G  II P+  +PYPF+    E  +P+IFGEW+N D E +  +  
Sbjct: 125 TLWYHAHISWLRSTVYGPLIILPKRGVPYPFAKPHKE--VPMIFGEWFNADTEAIIRQAT 184

Query: 191 RSGGGPNNSDAYTINGLPGPLYPCSSTDTFISTVERGKTYLLRVINGALNDELFFAIANH 250
           ++GGGPN SDAYTINGLPGPLY CS+ DTF   V+ GKTYLLR+IN ALNDELFF+IANH
Sbjct: 185 QTGGGPNVSDAYTINGLPGPLYNCSAKDTFRLRVKPGKTYLLRLINAALNDELFFSIANH 244

Query: 251 TLTVVEIDAAYTKPFNTTAIMIAPGQTTTLLLNTDQIQIPDHSSEFLFPMAITPYVTSNF 310
           T+TVVE DA Y KPF T  I+IAPGQTT +LL T +   P  S    F M   PYVT   
Sbjct: 245 TVTVVEADAIYVKPFETETILIAPGQTTNVLLKT-KSSYPSAS----FFMTARPYVTGQG 304

Query: 311 PFNNSTSIGFLRYKSRKMNKLKRQKKFPSN--QIPENLPDMKDTAFATAFSSKLRSLNSS 370
            F+NST  G L Y+  K  K    +    N       LP + DT FAT FS+KLRSLNS 
Sbjct: 305 TFDNSTVAGILEYEPPKQTKGAHSRTSIKNLQLFKPILPALNDTNFATKFSNKLRSLNSK 364

Query: 371 LYPCNVPKTVHKRVFVTISLNLQNC--PSGKTCKG-LNGKRFFASMNNQSFIRPDSSILE 430
            +P NVP  V ++ F T+ L    C   + +TC+G  N   F AS++N SF  P  ++L+
Sbjct: 365 NFPANVPLNVDRKFFFTVGLGTNPCNHKNNQTCQGPTNTTMFAASISNISFTMPTKALLQ 424

Query: 431 SHYRKIDTNSYSTDFPEKPIRFFDYTGVNPLSRNMNTEFGTKLLAVPYGTNLEIVFQGTN 490
           SHY       YS  FP  PI  F+YTG  P   N     GT L+ +PY T++E+V Q T+
Sbjct: 425 SHYSGQSHGVYSPKFPWSPIVPFNYTGTPP--NNTMVSNGTNLMVLPYNTSVELVMQDTS 484

Query: 491 FLSVENHPIHVHGHNFFVVGRGFGNFNVGKDPANYNLVDPPERNTVAVPRGGWAAIRIKA 550
            L  E+HP+H+HG NFFVVG+GFGNF+  KDP N+NLVDP ERNTV VP GGWAAIR  A
Sbjct: 485 ILGAESHPLHLHGFNFFVVGQGFGNFDPNKDPRNFNLVDPIERNTVGVPSGGWAAIRFLA 544

Query: 551 DNPGVWFIHCHLEEHTSWGLAMGLIVRNGAGDSKSLLPPPDDLPLC 590
           DNPGVWF+HCHLE HTSWGL M  +V +G    + LLPPP DLP C
Sbjct: 545 DNPGVWFMHCHLEVHTSWGLRMAWLVLDGDKPDQKLLPPPADLPKC 577

BLAST of Cucsa.121840 vs. TAIR10
Match: AT5G03260.1 (AT5G03260.1 laccase 11)

HSP 1 Score: 597.0 bits (1538), Expect = 1.2e-170
Identity = 297/584 (50.86%), Postives = 386/584 (66.10%), Query Frame = 1

Query: 10  MMLLVIFISILLAGFVPFSFASPVIRRFQFNVEWKKVTRLCHTKQLLTVNGQYPGPTIAV 69
           M +  +F+   L  F+ +S     ++++QF+V+ K ++R+C+ K ++TVNG +PGPT+  
Sbjct: 1   MKMGFLFLFCYLLAFLGYSPVDAAVKKYQFDVQVKNISRICNAKPIVTVNGMFPGPTVYA 60

Query: 70  HEGDTVEIKVNNCINENTTIHWHGVKQLRTGWADGPAYITQCPIRTGESYTYKFSVIDQR 129
            EGD V I V N +  N +IHWHG+KQ R GWADGPAYITQCPI+TG+SY Y F+V  QR
Sbjct: 61  REGDRVIINVTNHVQYNMSIHWHGLKQYRNGWADGPAYITQCPIQTGQSYLYDFNVTGQR 120

Query: 130 GTLWWHAHYSWQRASVHGAFIIYPR--MPYPFSTFPIEAGIPLIFGEWWNGDVEEVENEM 189
           GTLWWHAH  W RA+V+GA +I P    PYPF     E+ I  I GEWWN DVE   N+ 
Sbjct: 121 GTLWWHAHILWLRATVYGAIVILPAPGKPYPFPQPYQESNI--ILGEWWNKDVETAVNQA 180

Query: 190 LRSGGGPNNSDAYTINGLPGPLYPCSSTDTFISTVERGKTYLLRVINGALNDELFFAIAN 249
            + G  P  SDA+TING PGPL+PCS   TF+   E GKTYLLR+IN ALNDELFF IA 
Sbjct: 181 NQLGAPPPMSDAHTINGKPGPLFPCSEKHTFVIEAEAGKTYLLRIINAALNDELFFGIAG 240

Query: 250 HTLTVVEIDAAYTKPFNTTAIMIAPGQTTTLLLNTDQIQIPDHSSEFLFPMAITPYVTSN 309
           H +TVVEIDA YTKPF T AI++ PGQTT +L+ TD+       S   + MA +P++ + 
Sbjct: 241 HNMTVVEIDAVYTKPFTTKAILLGPGQTTNVLVKTDR-------SPNRYFMAASPFMDAP 300

Query: 310 FPFNNSTSIGFLRYKSRKMNKLKRQKKFPSNQIP--ENLPDMKDTAFATAFSSKLRSLNS 369
              +N T    L+YK             P+  +P    LP   DT+FA  ++ KL+SLN+
Sbjct: 301 VSVDNKTVTAILQYKG-----------VPNTVLPILPKLPLPNDTSFALDYNGKLKSLNT 360

Query: 370 SLYPCNVPKTVHKRVFVTISLNLQNCPSGKTCKGLNGKRFFASMNNQSFIRPDSSILESH 429
             +P  VP  V +R+F TI L +  CP   TC  +NG    AS+NN +FI P +++L++H
Sbjct: 361 PNFPALVPLKVDRRLFYTIGLGINACP---TC--VNGTNLAASINNITFIMPKTALLKAH 420

Query: 430 YRKIDTNSYSTDFPEKPIRFFDYTGVNPLSRNMNTEFGTKLLAVPYGTNLEIVFQGTNFL 489
           Y  I +  + TDFP++P + F+YTGV PL+ N+ T  GT+L  V + T +E+V Q TN L
Sbjct: 421 YSNI-SGVFRTDFPDRPPKAFNYTGV-PLTANLGTSTGTRLSRVKFNTTIELVLQDTNLL 480

Query: 490 SVENHPIHVHGHNFFVVGRGFGNFNVGKDPANYNLVDPPERNTVAVPRGGWAAIRIKADN 549
           +VE+HP H+HG+NFFVVG G GNF+  KDPA +NLVDPPERNTV VP GGWAAIR +ADN
Sbjct: 481 TVESHPFHLHGYNFFVVGTGVGNFDPKKDPAKFNLVDPPERNTVGVPTGGWAAIRFRADN 540

Query: 550 PGVWFIHCHLEEHTSWGLAMGLIVRNGAGDSKSLLPPPDDLPLC 590
           PGVWF+HCHLE HT WGL M  +V NG     S+LPPP D P C
Sbjct: 541 PGVWFMHCHLEVHTMWGLKMAFVVENGETPELSVLPPPKDYPSC 557

BLAST of Cucsa.121840 vs. TAIR10
Match: AT2G29130.1 (AT2G29130.1 laccase 2)

HSP 1 Score: 590.9 bits (1522), Expect = 8.8e-169
Identity = 287/561 (51.16%), Postives = 377/561 (67.20%), Query Frame = 1

Query: 33  VIRRFQFNVEWKKVTRLCHTKQLLTVNGQYPGPTIAVHEGDTVEIKVNNCINENTTIHWH 92
           + R +QF+++ K +TRLC TK ++TVNG++PGP +   EGD ++IKV N ++ N +IHWH
Sbjct: 28  ITRHYQFDIQLKNITRLCKTKTIVTVNGKFPGPRVTAREGDNLQIKVVNHVSNNISIHWH 87

Query: 93  GVKQLRTGWADGPAYITQCPIRTGESYTYKFSVIDQRGTLWWHAHYSWQRASVHGAFIIY 152
           G++QLR+GWADGP+Y+TQCPIR G+SY Y F+V  QRGTLWWHAH  W RA+V+G  II 
Sbjct: 88  GIRQLRSGWADGPSYVTQCPIRMGQSYVYNFTVTGQRGTLWWHAHIQWMRATVYGPLIIL 147

Query: 153 PRM--PYPFSTFPIEAGIPLIFGEWWNGDVEEVENEMLRSGGGPNNSDAYTINGLPGPLY 212
           P++  PYPF   P +  +P++FGEW+N D + V  + L++G GPN SDA+T NGLPGPLY
Sbjct: 148 PKLHQPYPFPK-PYKQ-VPILFGEWFNADPQAVVQQALQTGAGPNASDAHTFNGLPGPLY 207

Query: 213 PCSSTDTFISTVERGKTYLLRVINGALNDELFFAIANHTLTVVEIDAAYTKPFNTTAIMI 272
            CS+ DT+   V+ GKTYLLR+IN ALNDELFF IANHTLTVVE DA Y KPF T  +++
Sbjct: 208 NCSTKDTYKLMVKPGKTYLLRLINAALNDELFFTIANHTLTVVEADACYVKPFQTNIVLL 267

Query: 273 APGQTTTLLLNTDQIQIPDHSSEFLFPMAITPYVTSNFPFNNSTSIGFLRYKSRKMNKLK 332
            PGQTT +LL T  I          F M   PY T     +N+T  G L+Y+    +  K
Sbjct: 268 GPGQTTNVLLKTKPIY-----PNATFYMLARPYFTGQGTIDNTTVAGILQYQ----HHTK 327

Query: 333 RQKKFPSNQIPENLPDMKDTAFATAFSSKLRSLNSSLYPCNVPKTVHKRVFVTISLNLQN 392
             K    + I  +LP +  T++A  F+   RSL SS +P NVPK V K+ F  I L    
Sbjct: 328 SSKNL--SIIKPSLPPINSTSYAANFTKMFRSLASSTFPANVPKVVDKQYFFAIGLGTNP 387

Query: 393 CPSGKTCKG-LNGKRFFASMNNQSFIRPD-SSILESHYRKIDTNSYSTDFPEKPIRFFDY 452
           CP  +TC+G  N  +F AS+NN SFI P+ +S+L+S++     N + TDFP  PI  F+Y
Sbjct: 388 CPKNQTCQGPTNTTKFAASINNVSFILPNKTSLLQSYFVGKSKNVFMTDFPTAPIIPFNY 447

Query: 453 TGVNPLSRNMNTEFGTKLLAVPYGTNLEIVFQGTNFLSVENHPIHVHGHNFFVVGRGFGN 512
           TG  P   N     GTK++ + Y T +E+V QGT+ L +E HPIH+HG NF+VVG+GFGN
Sbjct: 448 TGTPP--NNTMVSRGTKVVVLKYKTTVELVLQGTSILGIEAHPIHLHGFNFYVVGQGFGN 507

Query: 513 FNVGKDPANYNLVDPPERNTVAVPRGGWAAIRIKADNPGVWFIHCHLEEHTSWGLAMGLI 572
           FN  +DP +YNLVDP ERNT+ +P GGW AIR  ADNPGVW +HCH+E H SWGL M  +
Sbjct: 508 FNPARDPKHYNLVDPVERNTINIPSGGWVAIRFLADNPGVWLMHCHIEIHLSWGLTMAWV 567

Query: 573 VRNGAGDSKSLLPPPDDLPLC 590
           V +G   ++ LLPPP D P C
Sbjct: 568 VLDGDLPNQKLLPPPSDFPKC 573

BLAST of Cucsa.121840 vs. TAIR10
Match: AT2G38080.1 (AT2G38080.1 Laccase/Diphenol oxidase family protein)

HSP 1 Score: 560.1 bits (1442), Expect = 1.7e-159
Identity = 282/581 (48.54%), Postives = 370/581 (63.68%), Query Frame = 1

Query: 11  MLLVIFISILLAGFVPFSFASPVIRRFQFNVEWKKVTRLCHTKQLLTVNGQYPGPTIAVH 70
           + LV F S+  A       +  ++R ++FNV  K VTRLC +K  +TVNG+YPGPTI   
Sbjct: 9   LFLVSFFSVFPAP------SESMVRHYKFNVVMKNVTRLCSSKPTVTVNGRYPGPTIYAR 68

Query: 71  EGDTVEIKVNNCINENTTIHWHGVKQLRTGWADGPAYITQCPIRTGESYTYKFSVIDQRG 130
           E DT+ IKV N +  N +IHWHGV+Q+RTGWADGPAYITQCPI+ G+ YTY +++  QRG
Sbjct: 69  EDDTLLIKVVNHVKYNVSIHWHGVRQVRTGWADGPAYITQCPIQPGQVYTYNYTLTGQRG 128

Query: 131 TLWWHAHYSWQRASVHGAFIIYPR--MPYPFSTFPIEAGIPLIFGEWWNGDVEEVENEML 190
           TLWWHAH  W RA+V+GA +I P+  +PYPF     E  I  + GEWW  D E + NE L
Sbjct: 129 TLWWHAHILWLRATVYGALVILPKRGVPYPFPKPDNEKVI--VLGEWWKSDTENIINEAL 188

Query: 191 RSGGGPNNSDAYTINGLPGPLYPCSSTDTFISTVERGKTYLLRVINGALNDELFFAIANH 250
           +SG  PN SD++ ING PGP+  C S    +S VE GKTYLLR++N ALN+ELFF +A H
Sbjct: 189 KSGLAPNVSDSHMINGHPGPVRNCPSQGYKLS-VENGKTYLLRLVNAALNEELFFKVAGH 248

Query: 251 TLTVVEIDAAYTKPFNTTAIMIAPGQTTTLLLNTDQIQIPDHSSEFLFPMAITPYVTSNF 310
             TVVE+DA Y KPF T  ++IAPGQTT +LL   +       S   + +  +P++ +  
Sbjct: 249 IFTVVEVDAVYVKPFKTDTVLIAPGQTTNVLLTASK-------SAGKYLVTASPFMDAPI 308

Query: 311 PFNNSTSIGFLRYKSRKMNKLKRQKKFPSNQIPENLPDMKDTAFATAFSSKLRSLNSSLY 370
             +N T+   + Y     +         S  I    P    T+ A  F++ LRSLNS  Y
Sbjct: 309 AVDNVTATATVHYSGTLSS---------SPTILTLPPPQNATSIANNFTNSLRSLNSKKY 368

Query: 371 PCNVPKTVHKRVFVTISLNLQNCPSGKTCKGLNGKRFFASMNNQSFIRPDSSILESHYRK 430
           P  VP T+   +F T+ L L  CP   TCK  NG R  AS+NN +FI P +++L +HY  
Sbjct: 369 PALVPTTIDHHLFFTVGLGLNACP---TCKAGNGSRVVASINNVTFIMPKTALLPAHYFN 428

Query: 431 IDTNSYSTDFPEKPIRFFDYTGVNPLSRNMNTEFGTKLLAVPYGTNLEIVFQGTNFLSVE 490
             +  ++TDFP+ P   F+Y+G +    NM TE GT+L  +PY   +++V Q T  ++ E
Sbjct: 429 -TSGVFTTDFPKNPPHVFNYSGGS--VTNMATETGTRLYKLPYNATVQLVLQDTGVIAPE 488

Query: 491 NHPIHVHGHNFFVVGRGFGNFNVGKDPANYNLVDPPERNTVAVPRGGWAAIRIKADNPGV 550
           NHP+H+HG NFF VGRG GNFN  KDP N+NLVDP ERNT+ VP GGW  IR +ADNPGV
Sbjct: 489 NHPVHLHGFNFFEVGRGLGNFNSTKDPKNFNLVDPVERNTIGVPSGGWVVIRFRADNPGV 548

Query: 551 WFIHCHLEEHTSWGLAMGLIVRNGAGDSKSLLPPPDDLPLC 590
           WF+HCHLE HT+WGL M  +V NG G ++S+LPPP DLP C
Sbjct: 549 WFMHCHLEVHTTWGLKMAFLVENGKGPNQSILPPPKDLPKC 558

BLAST of Cucsa.121840 vs. NCBI nr
Match: gi|778657338|ref|XP_004137624.2| (PREDICTED: laccase-1 [Cucumis sativus])

HSP 1 Score: 1226.1 bits (3171), Expect = 0.0e+00
Identity = 588/589 (99.83%), Postives = 588/589 (99.83%), Query Frame = 1

Query: 1   MKSLSQQCRMMLLVIFISILLAGFVPFSFASPVIRRFQFNVEWKKVTRLCHTKQLLTVNG 60
           MKSLSQQCRMMLLVIFISILLAGFVPFSFASPVIRRFQFNVEWKKVTRLCHTKQLLTVNG
Sbjct: 3   MKSLSQQCRMMLLVIFISILLAGFVPFSFASPVIRRFQFNVEWKKVTRLCHTKQLLTVNG 62

Query: 61  QYPGPTIAVHEGDTVEIKVNNCINENTTIHWHGVKQLRTGWADGPAYITQCPIRTGESYT 120
           QYPGPTIAVHEGDTVEIKVNNCINENTTIHWHGVKQLRTGWADGPAYITQCPIRTGESYT
Sbjct: 63  QYPGPTIAVHEGDTVEIKVNNCINENTTIHWHGVKQLRTGWADGPAYITQCPIRTGESYT 122

Query: 121 YKFSVIDQRGTLWWHAHYSWQRASVHGAFIIYPRMPYPFSTFPIEAGIPLIFGEWWNGDV 180
           YKFSVIDQRGTLWWHAHYSWQRASVHGAFIIYPRMPYPFSTFPIEAGIPLIFGEWWNGDV
Sbjct: 123 YKFSVIDQRGTLWWHAHYSWQRASVHGAFIIYPRMPYPFSTFPIEAGIPLIFGEWWNGDV 182

Query: 181 EEVENEMLRSGGGPNNSDAYTINGLPGPLYPCSSTDTFISTVERGKTYLLRVINGALNDE 240
           EEVENEMLRSGGGPNNSDAYTINGLPGPLYPCSSTDTFISTVERGKTYLLRVINGALNDE
Sbjct: 183 EEVENEMLRSGGGPNNSDAYTINGLPGPLYPCSSTDTFISTVERGKTYLLRVINGALNDE 242

Query: 241 LFFAIANHTLTVVEIDAAYTKPFNTTAIMIAPGQTTTLLLNTDQIQIPDHSSEFLFPMAI 300
           LFFAIANHTLTVVEIDAAYTKPFNTTAIMIAPGQTTTLLLNTDQIQIPDHSSEFLFPMAI
Sbjct: 243 LFFAIANHTLTVVEIDAAYTKPFNTTAIMIAPGQTTTLLLNTDQIQIPDHSSEFLFPMAI 302

Query: 301 TPYVTSNFPFNNSTSIGFLRYKSRKMNKLKRQKKFPSNQIPENLPDMKDTAFATAFSSKL 360
           TPYVTSNFPFNNSTSIGFLRYKSRKMNKLKRQKKFPSNQIPENLPDMKDTAFATAFSSKL
Sbjct: 303 TPYVTSNFPFNNSTSIGFLRYKSRKMNKLKRQKKFPSNQIPENLPDMKDTAFATAFSSKL 362

Query: 361 RSLNSSLYPCNVPKTVHKRVFVTISLNLQNCPSGKTCKGLNGKRFFASMNNQSFIRPDSS 420
           RSLNSSLYPCNVPKTVHKRVFVTISLNLQNCPSGKTCKGLNGKRFFASMNNQSFIRPDSS
Sbjct: 363 RSLNSSLYPCNVPKTVHKRVFVTISLNLQNCPSGKTCKGLNGKRFFASMNNQSFIRPDSS 422

Query: 421 ILESHYRKIDTNSYSTDFPEKPIRFFDYTGVNPLSRNMNTEFGTKLLAVPYGTNLEIVFQ 480
           ILESHYRKIDTNSYSTDFPEKPIRFF YTGVNPLSRNMNTEFGTKLLAVPYGTNLEIVFQ
Sbjct: 423 ILESHYRKIDTNSYSTDFPEKPIRFFYYTGVNPLSRNMNTEFGTKLLAVPYGTNLEIVFQ 482

Query: 481 GTNFLSVENHPIHVHGHNFFVVGRGFGNFNVGKDPANYNLVDPPERNTVAVPRGGWAAIR 540
           GTNFLSVENHPIHVHGHNFFVVGRGFGNFNVGKDPANYNLVDPPERNTVAVPRGGWAAIR
Sbjct: 483 GTNFLSVENHPIHVHGHNFFVVGRGFGNFNVGKDPANYNLVDPPERNTVAVPRGGWAAIR 542

Query: 541 IKADNPGVWFIHCHLEEHTSWGLAMGLIVRNGAGDSKSLLPPPDDLPLC 590
           IKADNPGVWFIHCHLEEHTSWGLAMGLIVRNGAGDSKSLLPPPDDLPLC
Sbjct: 543 IKADNPGVWFIHCHLEEHTSWGLAMGLIVRNGAGDSKSLLPPPDDLPLC 591

BLAST of Cucsa.121840 vs. NCBI nr
Match: gi|659066823|ref|XP_008463071.1| (PREDICTED: LOW QUALITY PROTEIN: laccase-1 [Cucumis melo])

HSP 1 Score: 1164.4 bits (3011), Expect = 0.0e+00
Identity = 562/590 (95.25%), Postives = 573/590 (97.12%), Query Frame = 1

Query: 1   MKSLSQQCRMMLLVIFISILLAGFVPFSFASPVIRRFQFNVEWKKVTRLCHTKQLLTVNG 60
           MKSLSQQCRMMLLVIFISILLAGFVPFSFASPVIRRFQFNVEWKKVTRLCHTKQLLTVNG
Sbjct: 3   MKSLSQQCRMMLLVIFISILLAGFVPFSFASPVIRRFQFNVEWKKVTRLCHTKQLLTVNG 62

Query: 61  QYPGPTIAVHEGDTVEIKVNNCINENTTIHWHGVKQLRTGWADGPAYITQCPIRTGESYT 120
           QYPGPTIAVHEGDTVEIKVNNCINENTTIHWHGVKQLRTGWADGPAYITQCPIR+GESYT
Sbjct: 63  QYPGPTIAVHEGDTVEIKVNNCINENTTIHWHGVKQLRTGWADGPAYITQCPIRSGESYT 122

Query: 121 YKFSVIDQRGTLWWHAHYSWQRASVHGAFIIYPRMPYPFSTFPIEAGIPLIFGEWWNGDV 180
           YKFSVIDQRGTLWWHAHYSWQRASVHGAFIIYPRMPYPFSTFPIEAGIPLIFGEWWNGDV
Sbjct: 123 YKFSVIDQRGTLWWHAHYSWQRASVHGAFIIYPRMPYPFSTFPIEAGIPLIFGEWWNGDV 182

Query: 181 EEVENEMLRSGGGPNNSDAYTINGLPGPLYPCSSTDTFISTVERGKTYLLRVINGALNDE 240
           EEVENEML+SGGGPNNSDAYTINGLPGPLYPCSS DTFISTVERGKTYLLRVINGALNDE
Sbjct: 183 EEVENEMLKSGGGPNNSDAYTINGLPGPLYPCSSRDTFISTVERGKTYLLRVINGALNDE 242

Query: 241 LFFAIANHTLTVVEIDAAYTKPFNTTAIMIAPGQTTTLLLNTDQIQIPDHSSEFLFPMAI 300
           LFFAIANHTLTVVEIDAAYTKPFNTTAIMIAPGQTTTLLLNTDQIQIPDHSS  LFPMAI
Sbjct: 243 LFFAIANHTLTVVEIDAAYTKPFNTTAIMIAPGQTTTLLLNTDQIQIPDHSSGILFPMAI 302

Query: 301 TPYVTSNFPFNNSTSIGFLRYKSRKMNKLKRQKKFPSNQIPE-NLPDMKDTAFATAFSSK 360
           TPYVTS+FPFNNSTSIGFLRYKSRKMNKLKR++   S+ I   NLPDMKDTAFATAFS+K
Sbjct: 303 TPYVTSSFPFNNSTSIGFLRYKSRKMNKLKRKQN--SHLIKYLNLPDMKDTAFATAFSNK 362

Query: 361 LRSLNSSLYPCNVPKTVHKRVFVTISLNLQNCPSGKTCKGLNGKRFFASMNNQSFIRPDS 420
           LRSLNS LYPCNVPKTVHKRVFVTISLNLQNCPS K+CKGLNGKRFFASMNNQSFIRPDS
Sbjct: 363 LRSLNSPLYPCNVPKTVHKRVFVTISLNLQNCPSRKSCKGLNGKRFFASMNNQSFIRPDS 422

Query: 421 SILESHYRKIDTNSYSTDFPEKPIRFFDYTGVNPLSRNMNTEFGTKLLAVPYGTNLEIVF 480
           SILESHYRKI TNSYSTDFPEKPI  FDYTGVNPL++NMNTEFGTKLLAVPYGTNLEIVF
Sbjct: 423 SILESHYRKIATNSYSTDFPEKPITVFDYTGVNPLTKNMNTEFGTKLLAVPYGTNLEIVF 482

Query: 481 QGTNFLSVENHPIHVHGHNFFVVGRGFGNFNVGKDPANYNLVDPPERNTVAVPRGGWAAI 540
           QGTNFLSVENHPIHVHGHNFFVVGRGFGNFN G+DPA YNLVDPPERNTVAVPRGGWAAI
Sbjct: 483 QGTNFLSVENHPIHVHGHNFFVVGRGFGNFNAGRDPAKYNLVDPPERNTVAVPRGGWAAI 542

Query: 541 RIKADNPGVWFIHCHLEEHTSWGLAMGLIVRNGAGDSKSLLPPPDDLPLC 590
           RIKADNPGVWFIHCHLEEHTSWGLAMGLIVRNGAGDSKSLLPPPDDLPLC
Sbjct: 543 RIKADNPGVWFIHCHLEEHTSWGLAMGLIVRNGAGDSKSLLPPPDDLPLC 590

BLAST of Cucsa.121840 vs. NCBI nr
Match: gi|700209067|gb|KGN64163.1| (hypothetical protein Csa_1G042710 [Cucumis sativus])

HSP 1 Score: 1031.6 bits (2666), Expect = 5.5e-298
Identity = 493/494 (99.80%), Postives = 493/494 (99.80%), Query Frame = 1

Query: 96  QLRTGWADGPAYITQCPIRTGESYTYKFSVIDQRGTLWWHAHYSWQRASVHGAFIIYPRM 155
           QLRTGWADGPAYITQCPIRTGESYTYKFSVIDQRGTLWWHAHYSWQRASVHGAFIIYPRM
Sbjct: 41  QLRTGWADGPAYITQCPIRTGESYTYKFSVIDQRGTLWWHAHYSWQRASVHGAFIIYPRM 100

Query: 156 PYPFSTFPIEAGIPLIFGEWWNGDVEEVENEMLRSGGGPNNSDAYTINGLPGPLYPCSST 215
           PYPFSTFPIEAGIPLIFGEWWNGDVEEVENEMLRSGGGPNNSDAYTINGLPGPLYPCSST
Sbjct: 101 PYPFSTFPIEAGIPLIFGEWWNGDVEEVENEMLRSGGGPNNSDAYTINGLPGPLYPCSST 160

Query: 216 DTFISTVERGKTYLLRVINGALNDELFFAIANHTLTVVEIDAAYTKPFNTTAIMIAPGQT 275
           DTFISTVERGKTYLLRVINGALNDELFFAIANHTLTVVEIDAAYTKPFNTTAIMIAPGQT
Sbjct: 161 DTFISTVERGKTYLLRVINGALNDELFFAIANHTLTVVEIDAAYTKPFNTTAIMIAPGQT 220

Query: 276 TTLLLNTDQIQIPDHSSEFLFPMAITPYVTSNFPFNNSTSIGFLRYKSRKMNKLKRQKKF 335
           TTLLLNTDQIQIPDHSSEFLFPMAITPYVTSNFPFNNSTSIGFLRYKSRKMNKLKRQKKF
Sbjct: 221 TTLLLNTDQIQIPDHSSEFLFPMAITPYVTSNFPFNNSTSIGFLRYKSRKMNKLKRQKKF 280

Query: 336 PSNQIPENLPDMKDTAFATAFSSKLRSLNSSLYPCNVPKTVHKRVFVTISLNLQNCPSGK 395
           PSNQIPENLPDMKDTAFATAFSSKLRSLNSSLYPCNVPKTVHKRVFVTISLNLQNCPSGK
Sbjct: 281 PSNQIPENLPDMKDTAFATAFSSKLRSLNSSLYPCNVPKTVHKRVFVTISLNLQNCPSGK 340

Query: 396 TCKGLNGKRFFASMNNQSFIRPDSSILESHYRKIDTNSYSTDFPEKPIRFFDYTGVNPLS 455
           TCKGLNGKRFFASMNNQSFIRPDSSILESHYRKIDTNSYSTDFPEKPIRFF YTGVNPLS
Sbjct: 341 TCKGLNGKRFFASMNNQSFIRPDSSILESHYRKIDTNSYSTDFPEKPIRFFYYTGVNPLS 400

Query: 456 RNMNTEFGTKLLAVPYGTNLEIVFQGTNFLSVENHPIHVHGHNFFVVGRGFGNFNVGKDP 515
           RNMNTEFGTKLLAVPYGTNLEIVFQGTNFLSVENHPIHVHGHNFFVVGRGFGNFNVGKDP
Sbjct: 401 RNMNTEFGTKLLAVPYGTNLEIVFQGTNFLSVENHPIHVHGHNFFVVGRGFGNFNVGKDP 460

Query: 516 ANYNLVDPPERNTVAVPRGGWAAIRIKADNPGVWFIHCHLEEHTSWGLAMGLIVRNGAGD 575
           ANYNLVDPPERNTVAVPRGGWAAIRIKADNPGVWFIHCHLEEHTSWGLAMGLIVRNGAGD
Sbjct: 461 ANYNLVDPPERNTVAVPRGGWAAIRIKADNPGVWFIHCHLEEHTSWGLAMGLIVRNGAGD 520

Query: 576 SKSLLPPPDDLPLC 590
           SKSLLPPPDDLPLC
Sbjct: 521 SKSLLPPPDDLPLC 534

BLAST of Cucsa.121840 vs. NCBI nr
Match: gi|700209067|gb|KGN64163.1| (hypothetical protein Csa_1G042710 [Cucumis sativus])

HSP 1 Score: 80.9 bits (198), Expect = 8.4e-12
Identity = 52/96 (54.17%), Postives = 59/96 (61.46%), Query Frame = 1

Query: 1  MKSLSQQCRMMLLVIFISILLAGFVPFSFASPVIRRFQFNVEWKKVTRLCHTKQLLTVNG 60
          MKSLSQQCRMMLLVIFISILLAGFVPFSFASPVIRRFQFN             QL T  G
Sbjct: 1  MKSLSQQCRMMLLVIFISILLAGFVPFSFASPVIRRFQFN-------------QLRT--G 60

Query: 61 QYPGPT----IAVHEGDTVEIKVNNCINENTTIHWH 93
             GP       +  G++   K  + I++  T+ WH
Sbjct: 61 WADGPAYITQCPIRTGESYTYKF-SVIDQRGTLWWH 80

HSP 2 Score: 887.9 bits (2293), Expect = 9.8e-255
Identity = 424/579 (73.23%), Postives = 478/579 (82.56%), Query Frame = 1

Query: 13  LVIFISILLAGFVPFSFASPVIRRFQFNVEWKKVTRLCHTKQLLTVNGQYPGPTIAVHEG 72
           L + + ++LAG +P  F S   +RFQFNVEWK VTRLC TK LLTVNG+YPGPTIAVHEG
Sbjct: 10  LFVVMLMILAGALPSGF-SRTTQRFQFNVEWKNVTRLCSTKPLLTVNGEYPGPTIAVHEG 69

Query: 73  DTVEIKVNNCINENTTIHWHGVKQLRTGWADGPAYITQCPIRTGESYTYKFSVIDQRGTL 132
           D VEIKV N I +NTTIHWHG++QLRTGWADGPAYITQCPIR G+SYTYKF+V  QRGTL
Sbjct: 70  DHVEIKVTNHIADNTTIHWHGIRQLRTGWADGPAYITQCPIRGGKSYTYKFTVQYQRGTL 129

Query: 133 WWHAHYSWQRASVHGAFIIYPRMPYPFSTFPIEAGIPLIFGEWWNGDVEEVENEMLRSGG 192
           WWHAHY+WQRASV+GAFII+PRMP+PFS  PI+   P+IFGEWWNGDV+EVENEM++ GG
Sbjct: 130 WWHAHYAWQRASVYGAFIIHPRMPFPFSA-PIQDEFPIIFGEWWNGDVDEVENEMVKYGG 189

Query: 193 GPNNSDAYTINGLPGPLYPCSSTDTFISTVERGKTYLLRVINGALNDELFFAIANHTLTV 252
           GPN SDAYTINGLPGP YPCS+ DTFI TVE GKTYLLRVIN ALNDELFFA+ANHTLTV
Sbjct: 190 GPNISDAYTINGLPGPFYPCSNKDTFIKTVEHGKTYLLRVINAALNDELFFAVANHTLTV 249

Query: 253 VEIDAAYTKPFNTTAIMIAPGQTTTLLLNTDQIQIPDHSSEFLFPMAITPYVTSNFPFNN 312
           VEIDA YTKPF  TAIM+APGQTT +LL  +Q+  PD S  FL  MA  PY+TS FPF+N
Sbjct: 250 VEIDAVYTKPFTATAIMVAPGQTTNVLLTANQV--PDSSGTFL--MAAWPYLTSVFPFDN 309

Query: 313 STSIGFLRYKSRKMNKLKRQKKFPSNQIPE--NLPDMKDTAFATAFSSKLRSLNSSLYPC 372
           ST+ GFLRYK+     +K  +K PS   P   NLP M+DT F T FS KLRSL S  YPC
Sbjct: 310 STTAGFLRYKNTVTENIKHPQKMPSTFEPNKYNLPKMEDTNFVTKFSDKLRSLASPQYPC 369

Query: 373 NVPKTVHKRVFVTISLNLQNCPSGKTCKGLNGKRFFASMNNQSFIRPDSSILESHYRKID 432
            VPKT+ KRV   +SLNLQ+CP+ KTCKG   KRFFASMNNQSF+RP  SILESHY K+ 
Sbjct: 370 TVPKTIDKRVVTVVSLNLQDCPANKTCKGYEDKRFFASMNNQSFVRPSLSILESHYNKLR 429

Query: 433 TNSYSTDFPEKPIRFFDYTGVNPLSRNMNTEFGTKLLAVPYGTNLEIVFQGTNFLSVENH 492
            N YSTDFPEKP++ FDYTGV+P+S NMNT+FGTK+L VP+GTNLEIV QGT+FL+VENH
Sbjct: 430 NNEYSTDFPEKPLKPFDYTGVDPVSENMNTQFGTKILEVPFGTNLEIVLQGTSFLNVENH 489

Query: 493 PIHVHGHNFFVVGRGFGNFNVGKDPANYNLVDPPERNTVAVPRGGWAAIRIKADNPGVWF 552
           PIHVHGHNFF+VGRGFGNFNV KDPA YNL+DPPERNTVAVP GGWAAIR KADNPGVWF
Sbjct: 490 PIHVHGHNFFIVGRGFGNFNVAKDPAKYNLIDPPERNTVAVPTGGWAAIRFKADNPGVWF 549

Query: 553 IHCHLEEHTSWGLAMGLIVRNGAGDSKSLLPPPDDLPLC 590
           IHCHLEEHTSWGLA GLIV+NG   S+ +LPPP DLP C
Sbjct: 550 IHCHLEEHTSWGLASGLIVQNGPRASQWVLPPPKDLPSC 582

BLAST of Cucsa.121840 vs. NCBI nr
Match: gi|595858577|ref|XP_007210772.1| (hypothetical protein PRUPE_ppa019880mg [Prunus persica])

HSP 1 Score: 882.1 bits (2278), Expect = 5.4e-253
Identity = 421/579 (72.71%), Postives = 473/579 (81.69%), Query Frame = 1

Query: 13  LVIFISILLAGFVPFSFASPVIRRFQFNVEWKKVTRLCHTKQLLTVNGQYPGPTIAVHEG 72
           L + + ++LAG +P  F S   R FQFNVEWK VTRLC TK LLTVNG+YPGPTIAVHEG
Sbjct: 10  LFVVMLMILAGALPSGF-SQTTRHFQFNVEWKNVTRLCSTKPLLTVNGEYPGPTIAVHEG 69

Query: 73  DTVEIKVNNCINENTTIHWHGVKQLRTGWADGPAYITQCPIRTGESYTYKFSVIDQRGTL 132
           D VEIKV N I +NTTIHWHG++QLRTGWADGPAYITQCPIR G+SYTYKF+V  QRGTL
Sbjct: 70  DHVEIKVTNHIADNTTIHWHGIRQLRTGWADGPAYITQCPIRGGKSYTYKFTVQYQRGTL 129

Query: 133 WWHAHYSWQRASVHGAFIIYPRMPYPFSTFPIEAGIPLIFGEWWNGDVEEVENEMLRSGG 192
           WWHAHY+WQRASV+GAFII+PRMP+PFS  PI+   P+IFGEWW GDV+EVENEM++ GG
Sbjct: 130 WWHAHYAWQRASVYGAFIIHPRMPFPFSA-PIQDEFPIIFGEWWIGDVDEVENEMVKYGG 189

Query: 193 GPNNSDAYTINGLPGPLYPCSSTDTFISTVERGKTYLLRVINGALNDELFFAIANHTLTV 252
           GPN SDAYTINGLPGP YPCS+ DTFI TVE GKTYLLR+IN ALNDELFFA+ANHTLTV
Sbjct: 190 GPNISDAYTINGLPGPFYPCSNKDTFIKTVEHGKTYLLRIINAALNDELFFAVANHTLTV 249

Query: 253 VEIDAAYTKPFNTTAIMIAPGQTTTLLLNTDQIQIPDHSSEFLFPMAITPYVTSNFPFNN 312
           VEIDA YTKPF  TAIM+APGQTT +LL  +Q+  PD S  FL  MA  PY+TS FPF+N
Sbjct: 250 VEIDAVYTKPFTATAIMVAPGQTTNVLLTANQV--PDSSGTFL--MAAWPYLTSVFPFDN 309

Query: 313 STSIGFLRYKSRKMNKLKRQKKFPSNQIPE--NLPDMKDTAFATAFSSKLRSLNSSLYPC 372
           ST+ GFLRYK+     +K  +K PS   P   NLP M+DT F T FS KLRSL S  YPC
Sbjct: 310 STTAGFLRYKNTVTENIKHPQKMPSTFEPNKYNLPKMEDTMFVTKFSDKLRSLASPQYPC 369

Query: 373 NVPKTVHKRVFVTISLNLQNCPSGKTCKGLNGKRFFASMNNQSFIRPDSSILESHYRKID 432
            VPKT+ KRV   +SLNLQ+CP+ KTCKG   KRFFASMNNQSF+RP  SILESHY K+ 
Sbjct: 370 TVPKTIDKRVVTVVSLNLQDCPANKTCKGYEDKRFFASMNNQSFVRPSLSILESHYNKLR 429

Query: 433 TNSYSTDFPEKPIRFFDYTGVNPLSRNMNTEFGTKLLAVPYGTNLEIVFQGTNFLSVENH 492
            N YSTDFPEKP++ FDYTGV+P S NMNT+F TK+L VPYGTNLEIV QGT+FL+VENH
Sbjct: 430 NNEYSTDFPEKPLKPFDYTGVDPASENMNTQFSTKILEVPYGTNLEIVLQGTSFLNVENH 489

Query: 493 PIHVHGHNFFVVGRGFGNFNVGKDPANYNLVDPPERNTVAVPRGGWAAIRIKADNPGVWF 552
           PIHVHGHNFF+VGRGFGNFNV KDP  YNL+DPPERNTVAVP GGWAAIR KADNPGVWF
Sbjct: 490 PIHVHGHNFFIVGRGFGNFNVAKDPTKYNLIDPPERNTVAVPTGGWAAIRFKADNPGVWF 549

Query: 553 IHCHLEEHTSWGLAMGLIVRNGAGDSKSLLPPPDDLPLC 590
           IHCHLEEHTSWGLA GLIV+NG   S+ +LPPP DLP C
Sbjct: 550 IHCHLEEHTSWGLASGLIVQNGPRASQRVLPPPKDLPSC 582