Cucsa.112140 (gene) Cucumber (Gy14) v1

BLAST of Cucsa.112140 vs. Swiss-Prot
Match: XLG3_ARATH (Extra-large guanine nucleotide-binding protein 3 OS=Arabidopsis thaliana GN=XLG3 PE=1 SV=1)

HSP 1 Score: 996.5 bits (2575), Expect = 1.8e-289
Identity = 504/874 (57.67%), Postives = 639/874 (73.11%), Query Frame = 1

Query: 6   DEENAWQEAIRRMLPAGAPLP-DEEHLDYSIAVEYQGPPVAHELPKIDPLDLESLSILSP 65
           DE  +W+E +R+MLP GAPLP D    DYSIA+EY GPP  H++P++ P+D+    + +P
Sbjct: 5   DEGESWKEMVRKMLPPGAPLPEDPSEFDYSIALEYTGPPPVHDIPRVSPVDVNP-RVNNP 64

Query: 66  SVVPISDVSSIPIVYPIAPRVSRFNRLSNGAVPRSPVKSQRSSSVPRVQLELHSGERSKF 125
             +P+S                   R++ G    S      S SV  V   LH+   S  
Sbjct: 65  IPLPVS-------------------RIAGGVTSSSGGSPASSESVVSV---LHNNPESSS 124

Query: 126 GNGVDEVFSSELSAQHLNSESEPAIVEGKLANTVTFVTPRDSEDDEEDVFSSPRSCTTDV 185
           G+      S     Q+ N    P +V+ K  +    +  R++ ++E++   +       V
Sbjct: 125 GSASVSPVSGH--RQNGNQVRRP-VVKFKPVDDHDRIEGREAAEEEDNNVEAETERERKV 184

Query: 186 MTSPALSHSRDKRAKKKGICSRCGRGNRLKEREACLVCDAQYCSNCLLKTMGSMPEGRKC 245
               A +  R K  KKK  C RCG+  + + +E C+VCD +YC NC+L+ MGSMPEGRKC
Sbjct: 185 HECTASTKRRKK--KKKSECYRCGKA-KWENKETCIVCDEKYCGNCVLRAMGSMPEGRKC 244

Query: 246 VGCIGRPIDELKRAVLGKCSRILSRVCSPLEIKQIMKAEKECPANQLRPEQLIVNERQLR 305
           V CIG+ IDE KR+ LGK SR+LSR+ SPLE+KQIMKAEKEC ANQLRPEQLIVN   L+
Sbjct: 245 VSCIGQAIDESKRSKLGKHSRVLSRLLSPLEVKQIMKAEKECTANQLRPEQLIVNGYPLK 304

Query: 306 PEELAEILGCAIPPQKLKPGRYWYDKDSGLWGKEGEKPDRIISSKLNVGGKLRADASKGS 365
           PEE+A++L C +PPQKLKPGRYWYDK+SGLWGKEGEKPDR+ISS LN  GKL  DAS G+
Sbjct: 305 PEEMADLLNCLLPPQKLKPGRYWYDKESGLWGKEGEKPDRVISSNLNFTGKLSPDASNGN 364

Query: 366 TKVFINGREITKVELRVLKLANVQCPRDTHFWVYDDGSYEEEGQNNIKGNIWGKASTRLL 425
           T+V+INGREITK+ELR+LKLANVQCPRDTHFWVYDDG YEEEGQNNI+GNIW KASTR +
Sbjct: 365 TEVYINGREITKLELRILKLANVQCPRDTHFWVYDDGRYEEEGQNNIRGNIWEKASTRFM 424

Query: 426 CSLFSLPVP---PVNLHAPKDDPTTISGRSVPDYLEHGRIHKLLLFGIEGSGTSTLFKQA 485
           C+LFSLPVP   P     P  +  T     VP+Y+EH +I KLLL GIEGSGTST+FKQA
Sbjct: 425 CALFSLPVPQGQPRGTVQPSSNYAT-----VPNYIEHKKIQKLLLLGIEGSGTSTIFKQA 484

Query: 486 RFLYGNKFSPEELQNIKLMIQSNVYKYLSTLLEGRERFEEEAIIEKKTGAVIEEKLDKKA 545
           +FLYGNKFS EELQ+IKLM+QSN+Y+YLS LL+GRERFEEEA+   +    +E     + 
Sbjct: 485 KFLYGNKFSVEELQDIKLMVQSNMYRYLSILLDGRERFEEEALSHTRGLNAVEGDSGGEE 544

Query: 546 GTAETGVTESKQCLYSINQRFKHFSDWLLNIMATGDLDAFFPAATREYAPIVDEMWKDSA 605
              E G   + Q +Y++N R KHFSDWLL+I+ATGDLDAFFPAATREYAP+V+E+WKD A
Sbjct: 545 ANDE-GTVTTPQSVYTLNPRLKHFSDWLLDIIATGDLDAFFPAATREYAPLVEEVWKDPA 604

Query: 606 LQETYRRRDELHSLPDVTKYFLDRVIEISSNEYEPSDKDILYAEGVSQSNGLAFMEFYFD 665
           +Q TYRR+DELH LPDV +YFL R +E+SSNEYEPS++DI+YAEGV+Q NGLAFMEF   
Sbjct: 605 IQATYRRKDELHFLPDVAEYFLSRAMEVSSNEYEPSERDIVYAEGVTQGNGLAFMEFSLS 664

Query: 666 DRSPVSELYGENLE-LPPPLTKYQLIQINSEGLVDGCKWLDMFEDVRAMIFCVSLSDYDQ 725
           D SP+SE Y EN + L  P  KYQLI++N++G+ D CKW++MFEDVRA+IFC+SLSDYDQ
Sbjct: 665 DHSPMSESYPENPDALSSPQPKYQLIRVNAKGMNDSCKWVEMFEDVRAVIFCISLSDYDQ 724

Query: 726 --MLSHSKGPL--QNKMLASRNLFEHLVRHPCFGNTPFLLLLNKYDAFEEKINQVPLSSC 785
             +   S G +  QNKM+ S+ LFE +V+HPCF +TPF+L+LNKYD FEEK+N+ PL+SC
Sbjct: 725 INITPESSGTVQYQNKMIQSKELFESMVKHPCFKDTPFILILNKYDQFEEKLNRAPLTSC 784

Query: 786 EWFQDFCPVKPHNNSQTLAQQAYYYIAVKFKELYFSISGQKLFVWHTRARERASVDEAFR 845
           +WF DFCPV+ +NN Q+LA QAY+Y+A+KFK LYFSI+GQKLFVW  RAR+RA+VDE F+
Sbjct: 785 DWFSDFCPVRTNNNVQSLAYQAYFYVAMKFKLLYFSITGQKLFVWQARARDRANVDEGFK 843

Query: 846 YIREVLKWEEEKNDSMYDIVGDESFYSTEISSSP 871
           Y+REVLKW+EEK +S  +  G++SFYST++SSSP
Sbjct: 845 YVREVLKWDEEKEESYLNGGGEDSFYSTDMSSSP 843

BLAST of Cucsa.112140 vs. Swiss-Prot
Match: XLG1_ARATH (Extra-large guanine nucleotide-binding protein 1 OS=Arabidopsis thaliana GN=XLG1 PE=1 SV=2)

HSP 1 Score: 713.4 bits (1840), Expect = 3.1e-204
Identity = 372/727 (51.17%), Postives = 492/727 (67.68%), Query Frame = 1

Query: 151 EGKLANTVTF--VTPRDSEDDEEDVFSSPRSCTTDVMTSPALSHSRDKRAKKKGICSRCG 210
           +GK    VTF  +   D  ++EE   +  R     V   P       +   KKG C RC 
Sbjct: 177 DGKKQPVVTFLGIASDDGFEEEESCSNLRRVRVVPVKKQP-------QTKGKKGSCYRCF 236

Query: 211 RGNRLKEREACLVCDAQYCSNCLLKTMGSMPEGRKCVGCIGRPIDELKRAVLGKCSRILS 270
           +G+R  E+E CLVCDA+YC++C+L+ MGSMPEGRKCV CIG PIDE KR  LGKCSR+L 
Sbjct: 237 KGSRFTEKEVCLVCDAKYCNSCVLRAMGSMPEGRKCVTCIGFPIDESKRGSLGKCSRMLK 296

Query: 271 RVCSPLEIKQIMKAEKECPANQLRPEQLIVNERQLRPEELAEILGCAIPPQKLKPGRYWY 330
           R+ + LE+KQIMK E+ C ANQL  E + VN + L PEEL  +  C+ PP+KLKPG YWY
Sbjct: 297 RLLNDLEVKQIMKTERFCEANQLPAEYVYVNGQPLYPEELVTLQTCSNPPKKLKPGDYWY 356

Query: 331 DKDSGLWGKEGEKPDRIISSKLNVGGKLRADASKGSTKVFINGREITKVELRVLKLANVQ 390
           DK SGLWGKEGEKP +IIS  LNVGG +  +AS G+T+VFINGREITKVELR+L+LA VQ
Sbjct: 357 DKVSGLWGKEGEKPYQIISPHLNVGGPISPEASNGNTQVFINGREITKVELRMLQLAGVQ 416

Query: 391 CPRDTHFWVYDDGSYEEEGQNNIKGNIWGKASTRLLCSLFSLPVPPVN-LHAPKDDPTTI 450
           C  + HFWV +DGSY+EEGQ N KG IWGKA T+LLC++ SLPVP  +  +A  +   + 
Sbjct: 417 CAGNPHFWVNEDGSYQEEGQKNTKGYIWGKAGTKLLCAVLSLPVPSKSTANASGEQLYSA 476

Query: 451 SGRSVPDYLEHGRIHKLLLFGIEGSGTSTLFKQARFLYGN-KFSPEELQNIKLMIQSNVY 510
           + RS+ D+LEH  + K+LL G  GSGTST+FKQA+ LY +  F  +E +NIK++IQ+NVY
Sbjct: 477 NSRSILDHLEHRTLQKILLVGNSGSGTSTIFKQAKILYKDVPFLEDERENIKVIIQTNVY 536

Query: 511 KYLSTLLEGRERFEEEAIIEKKTGAVIEEKLDKKAGTAETGVTESKQCLYSINQRFKHFS 570
            YL  LLEGRERFEEEA+  + T   + E +    G A++   +    +YSI  R K FS
Sbjct: 537 GYLGMLLEGRERFEEEALALRNTKQCVLENIPADEGDAKS--NDKTVTMYSIGPRLKAFS 596

Query: 571 DWLLNIMATGDLDAFFPAATREYAPIVDEMWKDSALQETYRRRDELHSLPDVTKYFLDRV 630
           DWLL  MA G+L   FPAA+REYAP+V+E+W+D+A+Q TY+RR EL  LP V  YFL+R 
Sbjct: 597 DWLLKTMAAGNLGVIFPAASREYAPLVEELWRDAAIQATYKRRSELGLLPSVASYFLERA 656

Query: 631 IEISSNEYEPSDKDILYAEGVSQSNGLAFMEFYFDDRSPVSELYGENLELPPPLTKYQLI 690
           I++ + +YEPSD DILYAEGV+ S+GLA ++F F   +    L  +  +    L +YQLI
Sbjct: 657 IDVLTPDYEPSDLDILYAEGVTSSSGLACLDFSFPQTASEENL--DPSDHHDSLLRYQLI 716

Query: 691 QINSEGLVDGCKWLDMFEDVRAMIFCVSLSDYDQMLSHSKGPLQNKMLASRNLFEHLVRH 750
           ++ S GL + CKW+DMFEDV  ++F VS+SDYDQ+         NKML ++ LFE ++ H
Sbjct: 717 RVPSRGLGENCKWIDMFEDVGMVVFVVSMSDYDQVSEDG----TNKMLLTKKLFESIITH 776

Query: 751 PCFGNTPFLLLLNKYDAFEEKINQVPLSSCEWFQDFCPV-----KPHNNSQTLAQQAYYY 810
           P F N  FLL+LNKYD  EEK+ +VPL+ CEWFQDF PV       +N + TL Q A+++
Sbjct: 777 PIFENMDFLLILNKYDLLEEKVERVPLARCEWFQDFNPVVSRHRGSNNGNPTLGQLAFHF 836

Query: 811 IAVKFKELYFSISGQKLFVWHTRARERASVDEAFRYIREVLKWEEEKNDSMYDIVGDESF 869
           +AVKFK  Y S++G+KLFV  +++ +  SVD + +   E+LKW EE+ +     + + S 
Sbjct: 837 MAVKFKRFYSSLTGKKLFVSSSKSLDPNSVDSSLKLAMEILKWSEERTNI---CMSEYSM 885

HSP 2 Score: 37.7 bits (86), Expect = 7.6e-01
Identity = 18/47 (38.30%), Postives = 30/47 (63.83%), Query Frame = 1

Query: 36 AVEYQGPPVAHELPKIDPLDLESLSILS-PSVVPISDVSSIPIVYPI 82
          A EY GPP+++ +P   P+++E + + +  S V ISD  S P++ PI
Sbjct: 12 AEEYDGPPLSYNIPCAVPINVEKIPVAAVVSPVCISDNMSFPVIQPI 58

BLAST of Cucsa.112140 vs. Swiss-Prot
Match: XLG2_ARATH (Extra-large guanine nucleotide-binding protein 2 OS=Arabidopsis thaliana GN=XLG2 PE=1 SV=1)

HSP 1 Score: 595.5 bits (1534), Expect = 9.4e-169
Identity = 368/905 (40.66%), Postives = 518/905 (57.24%), Query Frame = 1

Query: 15  IRRMLPAGAPLPDEEH--------------LDYSIAVEYQGPPVAHELPKIDPLDLESLS 74
           IR++LP  +P P  ++              ++YS A EY+GP +A+ +P+  P++++ + 
Sbjct: 5   IRKLLPFPSPNPKRDNRESDDDDETSSGYRIEYSFASEYKGPLIAN-VPRALPVEVDQI- 64

Query: 75  ILSPSVVPISDVS-SIPIVYPIAPRVSRFN--RLSNGAVPRSP--VKSQRSSSVPRVQLE 134
              P+ +P+S  S    I YP+AP V   +  R  +  + +    V S   SSV  +  +
Sbjct: 65  ---PTALPVSFSSLRSGISYPVAPLVMTKDTKRPPDSGIEKKNGFVDSAAGSSVVLIGRD 124

Query: 135 LHSGERSKFGNG----VDEVFS------SELSAQHLNSESEPAIVEGKLANT----VTFV 194
           + SG  S   +      +EV S      S  S    ++  E  + + ++++     V FV
Sbjct: 125 VVSGSSSSSSSKRLDVPEEVKSPADFRLSPSSPLSASAREEDHLDDDRVSDVGPRAVRFV 184

Query: 195 TPRDSEDDEEDVFSSPRSCTTDVMTSPALSHSRDKRAKKKGICSRCGRGNRLKEREACLV 254
            P  S + +E  + S          S A +H R +R  K+G C RC  GNR  E+E C+V
Sbjct: 185 EPFQSSECDESSYVSDGE-------SIAATH-RAERKGKRGSCYRCQLGNRFTEKEVCIV 244

Query: 255 CDAQYCSNCLLKTMGSMPEGRKCVGCIGRPIDELKRAVLGKCSRILSRVCSPLEIKQIMK 314
           CDA+YC NC+ + MG+MPEGRKC  CIG  IDE KRA LGKCSR+L R  +  E++Q+M 
Sbjct: 245 CDAKYCFNCVRRAMGAMPEGRKCQACIGYRIDESKRASLGKCSRMLKRHLTDSELRQVMN 304

Query: 315 AEKECPANQLRPEQLIVNERQLRPEELAEILGCAIPPQKLKPGRYWYDKDSGLWGKEGEK 374
           AE  C ANQL    +IVN++ L  +EL  +  C  PP+KLKPG YWYDK +G WGK GEK
Sbjct: 305 AEITCKANQLPSRLIIVNDKPLSEDELYTLQTCPNPPKKLKPGHYWYDKVAGYWGKIGEK 364

Query: 375 PDRIISSKLNVGGKLRADASKGSTKVFINGREITKVELRVLKLANVQCPRDTHFWVYDDG 434
           P +IIS   ++GG +    S G T+++INGREITK EL +LK A VQC    HFWV  DG
Sbjct: 365 PSQIISPNNSIGGYISEKVSNGDTEIYINGREITKPELTMLKWAGVQCEGKPHFWVDSDG 424

Query: 435 SYEEEGQNNIKGNIWGKASTRLLCSLFSLPVPPVNLHAPKDDPTTISGRSVPDYLEHGRI 494
           SY EEGQ +  GNIW K   ++ C++FSLPVPP +        + +    VP Y E   +
Sbjct: 425 SYREEGQKHPIGNIWSKKRAKIACAVFSLPVPPAS--------SAVEPYDVPLY-EQKML 484

Query: 495 HKLLLFGIEGSGTSTLFKQARFLYGNKFSPEELQNIKLMIQSNVYKYLSTLLEGRERFEE 554
           +KLLL G E  G +T++KQAR LY   FS E+ + IK +IQ+N+Y YL+ +LE  ERFE+
Sbjct: 485 NKLLLIGSEKGGATTIYKQARSLYNVSFSLEDRERIKFIIQTNLYTYLAMVLEAHERFEK 544

Query: 555 EAIIEKKTGAVIEEKLDKKAGTAETGVTESKQCLYSINQRFKHFSDWLLNIMATGDLDAF 614
           E   ++ +G V +E   K                 SIN R KHFSDW+L     G+L   
Sbjct: 545 EMSNDQSSGNVGDETSAKPGN--------------SINPRLKHFSDWVLKEKEDGNL-KI 604

Query: 615 FPAATREYAPIVDEMWKDSALQETYRRRDELHSLPDVTKYFLDRVIEISSNEYEPSDKDI 674
           FP ++RE A  V ++W+  A+Q TY+R  +  +LP    YFL+R++EIS +EY+PSD DI
Sbjct: 605 FPPSSRENAQTVADLWRVPAIQATYKRLRD--TLPRNAVYFLERILEISRSEYDPSDMDI 664

Query: 675 LYAEGVSQSNGLAFMEFYFDDRSPVSEL---YGENLELPPPLTKYQLIQINSEGLVDGCK 734
           L AEG+S   GL+ ++F F   S    L   Y  + ++     KYQLI++N   L +  K
Sbjct: 665 LQAEGLSSMEGLSCVDFSFPSTSQEESLESDYQHDTDM-----KYQLIRLNPRSLGENWK 724

Query: 735 WLDMFEDVRAMIFCVSLSDYDQMLSHSKGPLQNKMLASRNLFEHLVRHPCFGNTPFLLLL 794
            L+MFED   +IFCVSL+DY + +   +G + NKMLA++ LFE++V HP   N  FLL+L
Sbjct: 725 LLEMFEDADLVIFCVSLTDYAENIEDGEGNIVNKMLATKQLFENMVTHPSLANKRFLLVL 784

Query: 795 NKYDAFEEKINQVPLSSCEWFQDFCPVKPHN----NSQTLAQQAYYYIAVKFKELYFSI- 854
            K+D  EEKI +VPL +CEWF+DF P+   N    ++  +AQ+A++YI  KFK LY SI 
Sbjct: 785 TKFDLLEEKIEEVPLRTCEWFEDFNPLISQNQTSRHNPPMAQRAFHYIGYKFKRLYDSIL 844

Query: 855 ---------SGQKLFVWHTRARERASVDEAFRYIREVLKWEEEKNDSMYDIVGDESFYST 870
                       KLFV    + E  +VD A RY RE+LKW  E+  SM+    + S  S 
Sbjct: 845 EPVNMRGRSFKPKLFVCQV-SLESDTVDNALRYAREILKWHVEET-SMFQ---EMSTTSI 860

BLAST of Cucsa.112140 vs. Swiss-Prot
Match: GPA2_SOYBN (Guanine nucleotide-binding protein alpha-2 subunit OS=Glycine max GN=GPA2 PE=2 SV=1)

HSP 1 Score: 195.3 bits (495), Expect = 2.8e-48
Identity = 130/388 (33.51%), Postives = 208/388 (53.61%), Query Frame = 1

Query: 461 IHKLLLFGIEGSGTSTLFKQARFLYGNKFSPEELQNIKLMIQSNVYKYLSTLLEGRERFE 520
           I KLLL G   SG ST+FKQ + L+   F   EL++   +I +NVY+ +  L +G + F 
Sbjct: 40  IQKLLLLGAGESGKSTIFKQIKLLFQTGFDEAELKSYLPVIHANVYQTIKLLHDGSKEF- 99

Query: 521 EEAIIEKKTGAVIEEKLDKKAGTAETGVTESKQCLYSINQRFKHFSDWLLNIMATGDLDA 580
                                  A+  V  SK   Y I+   K   + LL I   G LD 
Sbjct: 100 -----------------------AQNDVDSSK---YVISNENKEIGEKLLEI--GGRLD- 159

Query: 581 FFPAATREYAPIVDEMWKDSALQETYRRRDELHSLPDVTKYFLDRVIEISSNEYEPSDKD 640
            +P  ++E A  ++ +WKD A+QETY R  EL  +PD T YF++ +  +S   Y P+ +D
Sbjct: 160 -YPYLSKELAQEIENLWKDPAIQETYARGSELQ-IPDCTDYFMENLQRLSDANYVPTKED 219

Query: 641 ILYAEGVSQSNGLAFMEFYFDDRSPVSELYGENLELPPPLTKYQLIQINSEGLVDGCKWL 700
           +LYA    ++ G+  ++F     SPV    GEN +       Y+L  +  +   +  KW+
Sbjct: 220 VLYAR--VRTTGVVEIQF-----SPV----GENKKSDEV---YRLFDVGGQRN-ERRKWI 279

Query: 701 DMFEDVRAMIFCVSLSDYDQMLSHSKGPLQNKMLASRNLFEHLVRHPCFGNTPFLLLLNK 760
            +FE V A+IFC ++S+YDQ L   +   +N+M+ ++ LFE +++ PCF  T F+L LNK
Sbjct: 280 HLFEGVSAVIFCAAISEYDQTLFEDEN--RNRMMETKELFEWILKQPCFEKTSFMLFLNK 339

Query: 761 YDAFEEKINQVPLSSCEWFQDFCPVKPHNNSQTLAQQAYYYIAVKFKELYFSISG----Q 820
           +D FE+KI +VPL+ CEWF+D+ PV   +  +   + AY ++  KF+E YF  +      
Sbjct: 340 FDIFEKKILKVPLNVCEWFKDYQPV---STGKQEIEHAYEFVKKKFEESYFQSTAPDRVD 375

Query: 821 KLF-VWHTRARERASVDEAFRYIREVLK 844
           ++F ++ T A ++  V + F+ + E L+
Sbjct: 400 RVFKIYRTTALDQKVVKKTFKLVDETLR 375

BLAST of Cucsa.112140 vs. Swiss-Prot
Match: GPA1_LOTJA (Guanine nucleotide-binding protein alpha-1 subunit OS=Lotus japonicus GN=GPA1 PE=2 SV=1)

HSP 1 Score: 191.0 bits (484), Expect = 5.4e-47
Identity = 129/388 (33.25%), Postives = 208/388 (53.61%), Query Frame = 1

Query: 461 IHKLLLFGIEGSGTSTLFKQARFLYGNKFSPEELQNIKLMIQSNVYKYLSTLLEGRERFE 520
           I KLLL G   SG ST+FKQ + L+   F   EL++ + +I +NVY+ +  L +G +   
Sbjct: 39  IQKLLLLGAGESGKSTIFKQIKLLFQTGFDEAELKSYQPVIHANVYQTIKLLHDGAKEL- 98

Query: 521 EEAIIEKKTGAVIEEKLDKKAGTAETGVTESKQCLYSINQRFKHFSDWLLNIMATGDLDA 580
                                  A+  V  SK   Y I+   K   + L  I   G LD 
Sbjct: 99  -----------------------AQNDVDFSK---YVISDENKEIGEKLSEI--GGRLD- 158

Query: 581 FFPAATREYAPIVDEMWKDSALQETYRRRDELHSLPDVTKYFLDRVIEISSNEYEPSDKD 640
            +P  T+E A  ++ +WKD+A+QETY R +EL  +PD T YF++ +  +S   Y P+  D
Sbjct: 159 -YPCLTKELALEIENLWKDAAIQETYARGNELQ-VPDCTHYFMENLHRLSDANYVPTKDD 218

Query: 641 ILYAEGVSQSNGLAFMEFYFDDRSPVSELYGENLELPPPLTKYQLIQINSEGLVDGCKWL 700
           +LYA    ++ G+  ++F     SPV    GEN +       Y+L  +  +   +  KW+
Sbjct: 219 VLYAR--VRTTGVVEIQF-----SPV----GENKKSGEV---YRLFDVGGQRN-ERRKWI 278

Query: 701 DMFEDVRAMIFCVSLSDYDQMLSHSKGPLQNKMLASRNLFEHLVRHPCFGNTPFLLLLNK 760
            +FE V A+IFC ++S+YDQ L   +   +N+M+ ++ LFE +++ PCF  T F+L LNK
Sbjct: 279 HLFEGVSAVIFCAAISEYDQTLFEDEN--KNRMMETKELFEWVLKQPCFEKTSFMLFLNK 338

Query: 761 YDAFEEKINQVPLSSCEWFQDFCPVKPHNNSQTLAQQAYYYIAVKFKELYFSISG----Q 820
           +D FE+KI +VPL+ CEWF+D+ PV   +  +   + AY ++  KF+E YF  +      
Sbjct: 339 FDIFEKKILKVPLNVCEWFKDYQPV---STGKQEIEHAYEFVKKKFEESYFQNTAPDRVD 374

Query: 821 KLF-VWHTRARERASVDEAFRYIREVLK 844
           ++F ++ T A ++  V + F+ + E L+
Sbjct: 399 RVFKIYRTTALDQKVVKKTFKLVDETLR 374

BLAST of Cucsa.112140 vs. TrEMBL
Match: M5WCX2_PRUPE (Uncharacterized protein OS=Prunus persica GN=PRUPE_ppa001215mg PE=4 SV=1)

HSP 1 Score: 1289.2 bits (3335), Expect = 0.0e+00
Identity = 638/882 (72.34%), Postives = 735/882 (83.33%), Query Frame = 1

Query: 1   MASDTDEENAWQEAIRRMLPAGAPLPDEEHLDYSIAVEYQGPPVAHE-LPKIDPLDLESL 60
           MAS+ ++E +W++ +R+MLPAGAPLPDEEHLDYSIAVEYQGPP+ ++ LP++DP+ ++SL
Sbjct: 1   MASEAEDEKSWKDLLRKMLPAGAPLPDEEHLDYSIAVEYQGPPLPYDDLPRVDPVQIDSL 60

Query: 61  SILSPSVVPISDVSSIPIVYPIAPRVSRFNRLSNGAVPRSPVKSQR-SSSVPRVQLELHS 120
              S + + +SD+SSIP+  P+A ++SRFNR+ NGA  + P  S   SSS P+ QL+L +
Sbjct: 61  QTSSVTSLSVSDLSSIPVAVPVAQKLSRFNRVRNGAGVKEPGSSSTTSSSAPKSQLDLQN 120

Query: 121 GERSKFGNGVDEVFSSELSAQHLNSESEP-AIVEGKLANTVTFVTPRDSE-------DDE 180
           G       G D+ FSSEL  Q  N + +P     GK A  VTF TPRDSE       D+E
Sbjct: 121 GREGSEFEGADQGFSSELPVQDSNPQQKPIGTTGGKRAAVVTFNTPRDSENEDDHENDNE 180

Query: 181 EDVFSSPRSCTTDVMTSP-ALSHSRDKRAKKKGICSRCGRGNRLKEREACLVCDAQYCSN 240
            D  SSP+S  T+ + SP AL+ S  +R  K+GICSRCG+GNRLKERE CLVCDA++CSN
Sbjct: 181 ADRSSSPQSSATEPLGSPIALASSPGRRTNKRGICSRCGKGNRLKEREWCLVCDAKFCSN 240

Query: 241 CLLKTMGSMPEGRKCVGCIGRPIDELKRAVLGKCSRILSRVCSPLEIKQIMKAEKECPAN 300
           CLLK MGSMPEGRKCV CIG+PIDE KR+ LGKCSRILSRVCSPLEI+QIMKAEKECPAN
Sbjct: 241 CLLKAMGSMPEGRKCVSCIGQPIDESKRSSLGKCSRILSRVCSPLEIRQIMKAEKECPAN 300

Query: 301 QLRPEQLIVNERQLRPEELAEILGCAIPPQKLKPGRYWYDKDSGLWGKEGEKPDRIISSK 360
           QLRPEQL+VN R LR EELAEILGC +PPQKLKPGRYWYDKDSGLWGKEGEKPDRIISSK
Sbjct: 301 QLRPEQLVVNGRLLREEELAEILGCELPPQKLKPGRYWYDKDSGLWGKEGEKPDRIISSK 360

Query: 361 LNVGGKLRADASKGSTKVFINGREITKVELRVLKLANVQCPRDTHFWVYDDGSYEEEGQN 420
           LNVGGKLR DAS G+TKVF+NGREITK E RVLKLA VQCP DTHFWVYDDGSYEEEGQN
Sbjct: 361 LNVGGKLRFDASNGNTKVFMNGREITKTERRVLKLAKVQCPPDTHFWVYDDGSYEEEGQN 420

Query: 421 NIKGNIWGKASTRLLCSLFSLPVPPVNLHAPKDDPTTISGRSVPDYLEHGRIHKLLLFGI 480
           NIKGNIWGKASTR +CSLFSLPVPP N H   +DPTT S RSVP+YLE GR+ KLLLFG+
Sbjct: 421 NIKGNIWGKASTRFICSLFSLPVPPGNQHGTNEDPTTPSSRSVPEYLEQGRVQKLLLFGL 480

Query: 481 EGSGTSTLFKQARFLYGNKFSPEELQNIKLMIQSNVYKYLSTLLEGRERFEEEAIIEKK- 540
           EGSGTST+FKQA+FLYGNKF+PEELQNIKLMIQSN+YKYLS LLEGRERFEEEA+ E + 
Sbjct: 481 EGSGTSTIFKQAKFLYGNKFTPEELQNIKLMIQSNMYKYLSILLEGRERFEEEALTENRA 540

Query: 541 TGAVIEEKLDKKAGTAETGVTESKQCLYSINQRFKHFSDWLLNIMATGDLDAFFPAATRE 600
           T +  EE        + + V ES+QC+Y+INQRFKHFSDWLL+IMATGDLDAFFPAATRE
Sbjct: 541 TSSAAEE--------SPSVVDESEQCIYAINQRFKHFSDWLLDIMATGDLDAFFPAATRE 600

Query: 601 YAPIVDEMWKDSALQETYRRRDELHSLPDVTKYFLDRVIEISSNEYEPSDKDILYAEGVS 660
           YAPIVDE+WKDSA+QETY+RR+ELH LP+V KYFLDR IEISSNEYEPS+ DILYAEGV+
Sbjct: 601 YAPIVDEVWKDSAIQETYKRREELHCLPEVAKYFLDRAIEISSNEYEPSENDILYAEGVT 660

Query: 661 QSNGLAFMEFYFDDRSPVSELYGENLELPPPLTKYQLIQINSEGLVDGCKWLDMFEDVRA 720
           QSNGLAFMEF FDDRS +SELY EN E PPPLTKYQLI+INS+GL DGCKWL+MFEDVRA
Sbjct: 661 QSNGLAFMEFSFDDRSAMSELYNENYECPPPLTKYQLIRINSKGLNDGCKWLEMFEDVRA 720

Query: 721 MIFCVSLSDYDQMLSHSKGPLQNKMLASRNLFEHLVRHPCFGNTPFLLLLNKYDAFEEKI 780
           +IFCV+LSDYDQM +   G L NKMLASR+LFE LVRHPCF NTPF+LLLNKYDAFE KI
Sbjct: 721 VIFCVALSDYDQMWAQGNGLLCNKMLASRDLFESLVRHPCFRNTPFVLLLNKYDAFEAKI 780

Query: 781 NQVPLSSCEWFQDFCPVKPHNNSQTLAQQAYYYIAVKFKELYFSISGQKLFVWHTRARER 840
           NQVPLS CEW +DF PVKPH N Q+LAQQAYYY+AVKFKELY SI+G+KLFV  TRAR  
Sbjct: 781 NQVPLSVCEWLKDFSPVKPHGNIQSLAQQAYYYVAVKFKELYSSITGEKLFVAQTRARAG 840

Query: 841 ASVDEAFRYIREVLKWEEEKNDSMYDIVGDESFYSTEISSSP 871
            SVDEAF+YIRE+LKW++EKND+ + + GD+SFYSTE+SSSP
Sbjct: 841 TSVDEAFKYIREILKWDDEKNDNFFAMNGDDSFYSTEMSSSP 874

BLAST of Cucsa.112140 vs. TrEMBL
Match: F6HW64_VITVI (Putative uncharacterized protein OS=Vitis vinifera GN=VIT_17s0119g00030 PE=4 SV=1)

HSP 1 Score: 1266.1 bits (3275), Expect = 0.0e+00
Identity = 633/889 (71.20%), Postives = 731/889 (82.23%), Query Frame = 1

Query: 1   MASDTDEENAWQEAIRRMLPAGAPLPDEEHLDYSIAVEYQGPPVAHELPKIDPLDLESLS 60
           MASD + E  W+E +R+MLP GAPLPDEEHLDYSIAVEY+GPPV + +P +DPLD++SLS
Sbjct: 1   MASDVEGEKLWEEVLRKMLPVGAPLPDEEHLDYSIAVEYEGPPVPYRVPIVDPLDVDSLS 60

Query: 61  ILSPSVVPIS--DVSSIPIVYPIAPRV-----SRFNRLSNGAVPR---SPVKSQRSSS-V 120
           I S S+V +S  D+ SIP+  PI P V     SRFNR+ NGA  R   SPV+S RSSS V
Sbjct: 61  IRSSSMVSVSASDLQSIPVAAPILPVVKVTNYSRFNRVRNGASLRELRSPVESGRSSSSV 120

Query: 121 PRVQLELHSGE--RSKFGNGVDEVFSSELSAQHLNSESEPA------IVEGKLANTVTFV 180
            R QL   + E   S  GNG  E+FSS  S Q  NS S         + EG+ +  VTF 
Sbjct: 121 WRGQLGSRNSEVEDSDCGNGRGELFSSASSVQDPNSVSTSTLASLKPVAEGRRSTMVTFN 180

Query: 181 TPRDSEDDEEDVFSSPRSCTTDVMTSPALSHSRDKRAKKKGICSRCGRGNRLKEREACLV 240
             R+SE DE++ FSSPRS   D   SP +  +++K  +K+G+CSRCG+ NRLKEREAC+V
Sbjct: 181 VARESEIDEDE-FSSPRSSVPDATDSPIMLRNQEKGTRKRGVCSRCGKRNRLKEREACIV 240

Query: 241 CDAQYCSNCLLKTMGSMPEGRKCVGCIGRPIDELKRAVLGKCSRILSRVCSPLEIKQIMK 300
           CDA+YC NCLLK MGSMPEGRKCV C+G+PI+E KR+ LGK SR+LS+ CSPLE+ QIMK
Sbjct: 241 CDARYCKNCLLKAMGSMPEGRKCVSCLGQPINESKRSSLGKYSRMLSKACSPLEVGQIMK 300

Query: 301 AEKECPANQLRPEQLIVNERQLRPEELAEILGCAIPPQKLKPGRYWYDKDSGLWGKEGEK 360
           AEKEC ANQLRPEQLIVN RQLR EELAE+LGC+IPPQKLKPGRYWYDKDSGLWGKEGEK
Sbjct: 301 AEKECLANQLRPEQLIVNGRQLRQEELAEVLGCSIPPQKLKPGRYWYDKDSGLWGKEGEK 360

Query: 361 PDRIISSKLNVGGKLRADASKGSTKVFINGREITKVELRVLKLANVQCPRDTHFWVYDDG 420
           PDRIISSKLN+GGKL+ DAS GSTKVFINGREITK+ELRVLK+ANVQCPRDTHFW+YDDG
Sbjct: 361 PDRIISSKLNIGGKLQLDASNGSTKVFINGREITKIELRVLKMANVQCPRDTHFWLYDDG 420

Query: 421 SYEEEGQNNIKGNIWGKASTRLLCSLFSLPVPPVNLHAPKDDPTTISGRSVPDYLEHGRI 480
           SYEEEGQNNIKGNIWGKASTR +CSLFSLPVPP     PK+DPT  S RSVP+YLE GR+
Sbjct: 421 SYEEEGQNNIKGNIWGKASTRFICSLFSLPVPPGIFQGPKEDPTAFSSRSVPEYLEQGRV 480

Query: 481 HKLLLFGIEGSGTSTLFKQARFLYGNKFSPEELQNIKLMIQSNVYKYLSTLLEGRERFEE 540
            KLLLFG+EGSGTSTLFKQA++LYGNKF+P+ELQ+IKLMIQSN+YKYLS LLEGRERFEE
Sbjct: 481 QKLLLFGLEGSGTSTLFKQAKYLYGNKFTPQELQSIKLMIQSNMYKYLSLLLEGRERFEE 540

Query: 541 EAIIEKKTGAVIEEKLDKKAGTAETGVTESKQCLYSINQRFKHFSDWLLNIMATGDLDAF 600
           EA++E+K      E  D      ETGV +SK C+YSINQ+FKHFSDWLL IMA GDLDA 
Sbjct: 541 EALMERKNNVSDAEGSD----PGETGVDQSKPCIYSINQKFKHFSDWLLGIMAMGDLDAI 600

Query: 601 FPAATREYAPIVDEMWKDSALQETYRRRDELHSLPDVTKYFLDRVIEISSNEYEPSDKDI 660
           FPAATREYAP+VDE+WKD A+QETY+RR+EL  LPDV KYFLDR IEISSNEYEPS KDI
Sbjct: 601 FPAATREYAPLVDELWKDPAIQETYKRREELLFLPDVAKYFLDRAIEISSNEYEPSQKDI 660

Query: 661 LYAEGVSQSNGLAFMEFYFDDRSPVSELYGENLELPPPLTKYQLIQINSEGLVDGCKWLD 720
           LYAEGV+QSNGLAFMEF FDDRSP+SE Y ENLE PPP +KYQLI+INS+GL DGCKWLD
Sbjct: 661 LYAEGVTQSNGLAFMEFSFDDRSPMSETYNENLECPPPSSKYQLIRINSKGLHDGCKWLD 720

Query: 721 MFEDVRAMIFCVSLSDYDQMLSHSKGPLQNKMLASRNLFEHLVRHPCFGNTPFLLLLNKY 780
           MFEDVRA+IFCV+LSDYD M + S G L NKMLASR+LFE LVRHPCF +T F+LLLNKY
Sbjct: 721 MFEDVRAVIFCVALSDYDHMYTDSAGSLYNKMLASRDLFESLVRHPCFIDTRFVLLLNKY 780

Query: 781 DAFEEKINQVPLSSCEWFQDFCPVKPHNNSQTLAQQAYYYIAVKFKELYFSISGQKLFVW 840
           DAFEEKIN+ PL+ CEWF +F PV+PH+N+Q+LA QAYYY+A+KFK+LY SIS +KLFVW
Sbjct: 781 DAFEEKINRSPLAVCEWFWEFSPVRPHHNNQSLAHQAYYYVAMKFKDLYHSISSRKLFVW 840

Query: 841 HTRARERASVDEAFRYIREVLKWEEEKNDSMYDIVGDESFYSTEISSSP 871
            TRARE +SVDEAF++IREVLKWEE+K+D+ YDI GDESFYSTE SSSP
Sbjct: 841 KTRAREPSSVDEAFKFIREVLKWEEDKDDNTYDITGDESFYSTEPSSSP 884

BLAST of Cucsa.112140 vs. TrEMBL
Match: V7C3E7_PHAVU (Uncharacterized protein OS=Phaseolus vulgaris GN=PHAVU_004G147400g PE=4 SV=1)

HSP 1 Score: 1254.6 bits (3245), Expect = 0.0e+00
Identity = 626/899 (69.63%), Postives = 742/899 (82.54%), Query Frame = 1

Query: 1   MASD-TDEENAWQEAIRRMLPAGAPLPDEEHLDYSIAVEYQGPPVAHELPKIDPLDLESL 60
           MAS+ T+E+ +W++ +RRMLPAGAPLPDEEHLDYSIAVEY+GPPVA+++PK+DPL++ + 
Sbjct: 1   MASESTEEDKSWEQVLRRMLPAGAPLPDEEHLDYSIAVEYEGPPVAYDVPKVDPLEIGTA 60

Query: 61  S------ILSPSVVPISDVS-SIPIVYPIAPRVSRFNRLSNGAVPR-------SPVKSQR 120
           +      I + S+V   + S SIP+  P+ PR SRF R+ NG   R       SPV+S+R
Sbjct: 61  ASGVAVPIRTASIVSDHNASASIPVAMPVHPRFSRFGRVRNGGFDRPPPPPQKSPVESRR 120

Query: 121 SSSVPRVQLEL--HSGE---RSKFGN--GVDEVFSS--ELSAQHLNSESEPA----IVEG 180
           SSSV R QL+   HSGE   RS +     VD+  SS  E+SAQH    S P        G
Sbjct: 121 SSSVSRSQLQFDSHSGEVVYRSDYSGEMNVDDGASSASEVSAQHSPPNSAPVPRGGSGAG 180

Query: 181 KLANTVTFVTPRDSEDDEEDVFSSPRS-CTTDVMTSPALSHSRDKRAKKKGICSRCGRGN 240
           K   TVTF TPRDSEDD+ D F SPRS  T+ V  SP+       R KK+ ICSRCG  N
Sbjct: 181 KRPTTVTFHTPRDSEDDDGDDFPSPRSVATSPVGASPS-------RNKKRWICSRCGNSN 240

Query: 241 RLKEREACLVCDAQYCSNCLLKTMGSMPEGRKCVGCIGRPIDELKRAVLGKCSRILSRVC 300
           RLKE+EACLVCD++YCSNC+LK MGSMPEGRKCV CIG+PIDE KR +LGKCSR+LS+VC
Sbjct: 241 RLKEKEACLVCDSRYCSNCVLKAMGSMPEGRKCVSCIGKPIDESKRPILGKCSRMLSKVC 300

Query: 301 SPLEIKQIMKAEKECPANQLRPEQLIVNERQLRPEELAEILGCAIPPQKLKPGRYWYDKD 360
           S LEI QIMKAEKECPANQLRPEQLIVN RQLR EELAEILGC+IPPQKLKPGRYWYDKD
Sbjct: 301 SSLEINQIMKAEKECPANQLRPEQLIVNGRQLRQEELAEILGCSIPPQKLKPGRYWYDKD 360

Query: 361 SGLWGKEGEKPDRIISSKLNVGGKLRADASKGSTKVFINGREITKVELRVLKLANVQCPR 420
           SGLWGKEGEKPD+IISSKLN+GGKL+ DAS G+T+V++NGREITK+ELR+LKLANVQCPR
Sbjct: 361 SGLWGKEGEKPDKIISSKLNIGGKLQTDASNGNTRVYMNGREITKIELRMLKLANVQCPR 420

Query: 421 DTHFWVYDDGSYEEEGQNNIKGNIWGKASTRLLCSLFSLPVPPVNLHAPKDDPTTISGRS 480
           DTHFWVY+DGSYEEEGQNNIKGNIWGKASTR +CSLFSLPVPP N    +D+ T  S RS
Sbjct: 421 DTHFWVYEDGSYEEEGQNNIKGNIWGKASTRFICSLFSLPVPPTNPPGVRDNSTNYSARS 480

Query: 481 VPDYLEHGRIHKLLLFGIEGSGTSTLFKQARFLYGNKFSPEELQNIKLMIQSNVYKYLST 540
           VP+YLE GR+ KLLLFG+EGSGT+TLFKQA+FLYGNKFS EELQNIKLMIQSN+YKYLS 
Sbjct: 481 VPEYLEQGRVQKLLLFGMEGSGTATLFKQAKFLYGNKFSTEELQNIKLMIQSNMYKYLSI 540

Query: 541 LLEGRERFEEEAIIEKKTGAVIEEKLDKKAGTAETGVTESKQCLYSINQRFKHFSDWLLN 600
           LLEGRE+FEEE++ E+++ ++  E         ET   E+K C+YSINQRFKHFSDWLL+
Sbjct: 541 LLEGREQFEEESLAERESTSLEGE---GSGQVPETAADENKPCIYSINQRFKHFSDWLLD 600

Query: 601 IMATGDLDAFFPAATREYAPIVDEMWKDSALQETYRRRDELHSLPDVTKYFLDRVIEISS 660
           IMATGDL+AFFPAATREYAP+VDE+W+D A+QETY+RR+ELH+LPDV KYFLDR IEISS
Sbjct: 601 IMATGDLEAFFPAATREYAPMVDEIWRDPAVQETYKRREELHNLPDVAKYFLDRAIEISS 660

Query: 661 NEYEPSDKDILYAEGVSQSNGLAFMEFYFDDRSPVSELYGENLELPPPLTKYQLIQINSE 720
           NEYEPSDKD+LYAEGV+QSNGLAFMEF FDDRSP+SE+Y ENL  PPPLTKYQLI+INS+
Sbjct: 661 NEYEPSDKDVLYAEGVTQSNGLAFMEFSFDDRSPMSEIYSENLNCPPPLTKYQLIRINSK 720

Query: 721 GLVDGCKWLDMFEDVRAMIFCVSLSDYDQMLSHSKGPLQNKMLASRNLFEHLVRHPCFGN 780
           GL DGCKWL+MFEDVRA+IFCV+LSDYDQM   S G L+NK+LAS++LFE LV+HPCF +
Sbjct: 721 GLRDGCKWLEMFEDVRAIIFCVALSDYDQMWPSSTGQLRNKLLASKDLFESLVKHPCFKD 780

Query: 781 TPFLLLLNKYDAFEEKINQVPLSSCEWFQDFCPVKPHNNSQTLAQQAYYYIAVKFKELYF 840
           TPF+LLLNKYDAFE+KIN+V LS+CEWF DFCPV+PH+N+  LA QAYYY+AVKFKELY+
Sbjct: 781 TPFVLLLNKYDAFEDKINKVHLSTCEWFGDFCPVRPHHNNHALAHQAYYYVAVKFKELYY 840

Query: 841 SISGQKLFVWHTRARERASVDEAFRYIREVLKWEEEKNDSMYDIVGDESFYSTEISSSP 871
            ++GQKLFV  TRARER SVDEAF+YIRE++KW++EK+D +Y++  +ESF STE+SSSP
Sbjct: 841 YLTGQKLFVGQTRARERTSVDEAFKYIREIIKWDDEKDDDVYEMNQEESFCSTEMSSSP 889

BLAST of Cucsa.112140 vs. TrEMBL
Match: A0A0S3TBX6_PHAAN (Uncharacterized protein OS=Vigna angularis var. angularis GN=Vigan.11G221500 PE=4 SV=1)

HSP 1 Score: 1241.9 bits (3212), Expect = 0.0e+00
Identity = 621/902 (68.85%), Postives = 734/902 (81.37%), Query Frame = 1

Query: 1   MASD-TDEENAWQEAIRRMLPAGAPLPDEEHLDYSIAVEYQGPPVAHELPKIDPLDLESL 60
           MAS+ T+E+ +W+  +RRMLPAGAPLPDEEHLDYSIAVEY+GPPV +++PK+DPL++ + 
Sbjct: 1   MASESTEEDKSWEHVLRRMLPAGAPLPDEEHLDYSIAVEYEGPPVPYDVPKVDPLEIGAT 60

Query: 61  SILSPSVVPISDVS---------SIPIVYPIAPRVSRFNRLSNGAV--------PRSPVK 120
           +  S   VPI   S         SIP+  P+ PR SRF R+ NG          PRSPV+
Sbjct: 61  A--SAVAVPIRTASIVSDHNASASIPVAMPVHPRFSRFGRVRNGGFDRAPPPPPPRSPVE 120

Query: 121 SQRSSSVPRVQLEL--HSGE---RSKFGNGVDEV----FSSELSAQHLNSESEPA----I 180
           S+RSSSV R Q +    SGE   RS +   V++      +SE S Q     S P      
Sbjct: 121 SRRSSSVSRSQSQFDSRSGEVVYRSDYSGEVNDEDGPSSASEASEQQSPPNSAPVPRSGA 180

Query: 181 VEGKLANTVTFVTPRDSEDDEEDVFSSPRSCTTDVMTSPALSHSRDKRAKKKGICSRCGR 240
             GK   TVTF TPRDSEDD+ D F+SPRS  T+ + SP    +   R KK+ ICSRCG 
Sbjct: 181 GAGKRPTTVTFHTPRDSEDDDGDDFTSPRSVATEPVGSPV--GASPSRNKKRWICSRCGN 240

Query: 241 GNRLKEREACLVCDAQYCSNCLLKTMGSMPEGRKCVGCIGRPIDELKRAVLGKCSRILSR 300
            NRLKE+EACLVCD++YCSNC+LK MGSMPEGRKCV CIG+PIDE KR  LGKCSR+LS+
Sbjct: 241 SNRLKEKEACLVCDSRYCSNCVLKAMGSMPEGRKCVSCIGKPIDESKRPSLGKCSRMLSK 300

Query: 301 VCSPLEIKQIMKAEKECPANQLRPEQLIVNERQLRPEELAEILGCAIPPQKLKPGRYWYD 360
           VCS LEI QIMKAEKECPANQLRPEQLIVN RQLR EELAEILGC IPPQKLKPGRYWYD
Sbjct: 301 VCSSLEINQIMKAEKECPANQLRPEQLIVNGRQLRQEELAEILGCPIPPQKLKPGRYWYD 360

Query: 361 KDSGLWGKEGEKPDRIISSKLNVGGKLRADASKGSTKVFINGREITKVELRVLKLANVQC 420
           KDSGLWGKEGEKPD+IISSKLN+GGKL+ DAS G+T+V++NGREITK+ELR+LKLANVQC
Sbjct: 361 KDSGLWGKEGEKPDKIISSKLNIGGKLQTDASNGNTRVYMNGREITKIELRMLKLANVQC 420

Query: 421 PRDTHFWVYDDGSYEEEGQNNIKGNIWGKASTRLLCSLFSLPVPPVNLHAPKDDPTTISG 480
           PRDTHFWVY+DGSYEEEGQNNIKGNIWGKASTR +CSLFSLPVPP N    +D+ T  S 
Sbjct: 421 PRDTHFWVYEDGSYEEEGQNNIKGNIWGKASTRFICSLFSLPVPPANPPGVRDNSTNYST 480

Query: 481 RSVPDYLEHGRIHKLLLFGIEGSGTSTLFKQARFLYGNKFSPEELQNIKLMIQSNVYKYL 540
           RSVP+YLE GR+ KLLLFG+EGSGT+TLFKQA+FLYGNKFS EELQNIKLMIQSN+YKYL
Sbjct: 481 RSVPEYLEQGRVQKLLLFGMEGSGTATLFKQAKFLYGNKFSTEELQNIKLMIQSNMYKYL 540

Query: 541 STLLEGRERFEEEAIIEKKTGAVIEEKLDKKAGTAETGVTESKQCLYSINQRFKHFSDWL 600
           S LLEGRE+FEEEA+ E+++ ++  E   +   TA  G   +K  +YSINQRFKHFSDWL
Sbjct: 541 SILLEGREQFEEEALAERESTSLEGEGSGQVQETAADG---NKPSIYSINQRFKHFSDWL 600

Query: 601 LNIMATGDLDAFFPAATREYAPIVDEMWKDSALQETYRRRDELHSLPDVTKYFLDRVIEI 660
           L+IMATGDL+AFFPAATREYAP+VDE+W+D A+QETY+RR+ELH+LPDV KYFLDR IEI
Sbjct: 601 LDIMATGDLEAFFPAATREYAPMVDEIWRDPAVQETYKRREELHNLPDVAKYFLDRAIEI 660

Query: 661 SSNEYEPSDKDILYAEGVSQSNGLAFMEFYFDDRSPVSELYGENLELPPPLTKYQLIQIN 720
           SSNEYEPSDKD+LYAEGV+QSNGLAFMEF FDDRSP+SE+Y ENL  PPPLTKYQLI+IN
Sbjct: 661 SSNEYEPSDKDVLYAEGVTQSNGLAFMEFSFDDRSPMSEIYSENLNCPPPLTKYQLIRIN 720

Query: 721 SEGLVDGCKWLDMFEDVRAMIFCVSLSDYDQMLSHSKGPLQNKMLASRNLFEHLVRHPCF 780
           S+GL DGCKWL+MFEDVRA+IFCV+LSDYDQM   S G L+NK+LAS++LFE LV+HPCF
Sbjct: 721 SKGLRDGCKWLEMFEDVRAIIFCVALSDYDQMWPSSNGQLRNKLLASKDLFESLVKHPCF 780

Query: 781 GNTPFLLLLNKYDAFEEKINQVPLSSCEWFQDFCPVKPHNNSQTLAQQAYYYIAVKFKEL 840
            +TPF+LLLNKYDAFE+KIN+V LS+CEWF DFCPV+PH+N+  LA QAYYY+AVKFKEL
Sbjct: 781 KDTPFVLLLNKYDAFEDKINKVSLSTCEWFGDFCPVRPHHNNHALAHQAYYYVAVKFKEL 840

Query: 841 YFSISGQKLFVWHTRARERASVDEAFRYIREVLKWEEEKNDSMYDIVGDE-SFYSTEISS 871
           Y+S++GQKLFV  TR R+R SVDEAF+YIRE++KW++EK+D +Y+I  +E SFYSTE+SS
Sbjct: 841 YYSLTGQKLFVGQTRGRDRTSVDEAFKYIREIIKWDDEKDDDVYEINPEESSFYSTEMSS 895

BLAST of Cucsa.112140 vs. TrEMBL
Match: K7LEG4_SOYBN (Uncharacterized protein OS=Glycine max GN=GLYMA_09G172000 PE=4 SV=1)

HSP 1 Score: 1236.5 bits (3198), Expect = 0.0e+00
Identity = 614/900 (68.22%), Postives = 731/900 (81.22%), Query Frame = 1

Query: 1   MASD-TDEENAWQEAIRRMLPAGAPLPDEEHLDYSIAVEYQGPPVAHELPKIDPLDLESL 60
           MAS+ T ++ +W+  +RRMLPAGAPLPDEEHLDYSIAVEY+GPPV +++PK+DPL + + 
Sbjct: 1   MASESTGDDKSWEHILRRMLPAGAPLPDEEHLDYSIAVEYEGPPVPYDVPKVDPLKIGAS 60

Query: 61  SILSPSVVPISDVS---------SIPIVYPIAPRVSRFNRLSNGA-----VPRSPVKSQR 120
           +      VPI   S         SIP+  P+ PR SRF+R+ NG      +PRSP +S+R
Sbjct: 61  T--GGGAVPIRTASIDSDHNAAASIPVAMPVHPRFSRFSRVRNGGFDRAPLPRSPAESRR 120

Query: 121 SSSVPRVQLEL--HSGE---RSKFGNGV----DEVFSSELSAQHLNSESEPAIVE----- 180
           SSSV R Q +    SGE   RS F   V    D+  SS  +A    +    A V      
Sbjct: 121 SSSVSRTQSQFDSRSGEVAYRSDFSGEVNADADDGASSASAASAPQTPPNSAPVPRSGTG 180

Query: 181 -GKLANTVTFVTPRDSEDDEEDVFSSPRSCTTDVMTSPALSHSRDKRAKKKGICSRCGRG 240
            GK   TVTF TPRDSEDD+ D + SPRS  T+ + SP  +     R KK+ ICSRCG  
Sbjct: 181 AGKRPTTVTFHTPRDSEDDDGDDYLSPRSVATEPVGSPLTASP--SRNKKRWICSRCGNS 240

Query: 241 NRLKEREACLVCDAQYCSNCLLKTMGSMPEGRKCVGCIGRPIDELKRAVLGKCSRILSRV 300
           NRLKE+EACLVCD++YCSNC+LK MGSMPEGRKCV CIG+PIDE+KR+ LGKCSR+LS+V
Sbjct: 241 NRLKEKEACLVCDSRYCSNCVLKAMGSMPEGRKCVSCIGKPIDEMKRSSLGKCSRMLSKV 300

Query: 301 CSPLEIKQIMKAEKECPANQLRPEQLIVNERQLRPEELAEILGCAIPPQKLKPGRYWYDK 360
           CS LEI QIMKAEKECPANQLRPEQL+VN RQLR EELAEILGC+IPPQKLKPGRYWYDK
Sbjct: 301 CSSLEINQIMKAEKECPANQLRPEQLVVNGRQLRQEELAEILGCSIPPQKLKPGRYWYDK 360

Query: 361 DSGLWGKEGEKPDRIISSKLNVGGKLRADASKGSTKVFINGREITKVELRVLKLANVQCP 420
           DSGLWGKEGEKPD+IISSKLN+GGKL+ DAS G+T+V++NGREITK+ELR+LKLANVQCP
Sbjct: 361 DSGLWGKEGEKPDKIISSKLNIGGKLQTDASDGNTRVYMNGREITKIELRMLKLANVQCP 420

Query: 421 RDTHFWVYDDGSYEEEGQNNIKGNIWGKASTRLLCSLFSLPVPPVNLHAPKDDPTTISGR 480
           RDTHFWVY+DGSYEEEGQNNIKGNIWGK STR +CSLFSLPVPP N    KD+ T  S R
Sbjct: 421 RDTHFWVYEDGSYEEEGQNNIKGNIWGKTSTRFICSLFSLPVPPTNPPGVKDNSTNHSTR 480

Query: 481 SVPDYLEHGRIHKLLLFGIEGSGTSTLFKQARFLYGNKFSPEELQNIKLMIQSNVYKYLS 540
           SVP+YLEHGR+ KLLLFG+EGSGT+TLFKQA+FLYGNKFS EELQNIKLMIQSN+YKYLS
Sbjct: 481 SVPEYLEHGRVQKLLLFGMEGSGTATLFKQAKFLYGNKFSAEELQNIKLMIQSNMYKYLS 540

Query: 541 TLLEGRERFEEEAIIEKKTGAVIEEKLDKKAGTAETGVTESKQCLYSINQRFKHFSDWLL 600
            LLEGRE+FEEEA+ E+++       L+ +    ET   E+K  +YSINQRFKHFSDWLL
Sbjct: 541 ILLEGREQFEEEALAERES-----TSLEGEGSGQETAADENKLSVYSINQRFKHFSDWLL 600

Query: 601 NIMATGDLDAFFPAATREYAPIVDEMWKDSALQETYRRRDELHSLPDVTKYFLDRVIEIS 660
           +IMATGDL+AFFPAATREYAP+VDE+W+D A+QETY+RR+ELH+LP+V KYFLDR IEIS
Sbjct: 601 DIMATGDLEAFFPAATREYAPMVDEIWRDPAVQETYKRREELHNLPNVAKYFLDRAIEIS 660

Query: 661 SNEYEPSDKDILYAEGVSQSNGLAFMEFYFDDRSPVSELYGENLELPPPLTKYQLIQINS 720
           SNEYEPSDKDILYAEGV+QSNGLAFMEF FDDRSP+SE+Y EN+  PP LTKYQLI+INS
Sbjct: 661 SNEYEPSDKDILYAEGVTQSNGLAFMEFSFDDRSPMSEIYSENINYPPHLTKYQLIRINS 720

Query: 721 EGLVDGCKWLDMFEDVRAMIFCVSLSDYDQMLSHSKGPLQNKMLASRNLFEHLVRHPCFG 780
           +GL DGCKWL+MFEDVRA+IFCVSLSDYDQM   S G L+NK+LAS++LFE LV+HPCF 
Sbjct: 721 KGLRDGCKWLEMFEDVRAIIFCVSLSDYDQMWPTSTGQLRNKLLASKDLFESLVKHPCFK 780

Query: 781 NTPFLLLLNKYDAFEEKINQVPLSSCEWFQDFCPVKPHNNSQTLAQQAYYYIAVKFKELY 840
           +TPF+LLLNKYD FE+KIN+VPLS CEWF DFCPV+PH+N+  LA QAYYY+A++FKELY
Sbjct: 781 DTPFVLLLNKYDVFEDKINKVPLSICEWFGDFCPVRPHHNNHALAHQAYYYVAMRFKELY 840

Query: 841 FSISGQKLFVWHTRARERASVDEAFRYIREVLKWEEEKNDSMYDIVGDESFYSTEISSSP 871
           +S++GQKLFV  TR R+R SVDEAF+YIRE++KW++EK++ +Y+I  +ESFYSTE+SSSP
Sbjct: 841 YSLTGQKLFVGQTRGRDRTSVDEAFKYIREIIKWDDEKDEDVYEINPEESFYSTEMSSSP 891

BLAST of Cucsa.112140 vs. TAIR10
Match: AT1G31930.1 (AT1G31930.1 extra-large GTP-binding protein 3)

HSP 1 Score: 996.5 bits (2575), Expect = 1.0e-290
Identity = 504/874 (57.67%), Postives = 639/874 (73.11%), Query Frame = 1

Query: 6   DEENAWQEAIRRMLPAGAPLP-DEEHLDYSIAVEYQGPPVAHELPKIDPLDLESLSILSP 65
           DE  +W+E +R+MLP GAPLP D    DYSIA+EY GPP  H++P++ P+D+    + +P
Sbjct: 5   DEGESWKEMVRKMLPPGAPLPEDPSEFDYSIALEYTGPPPVHDIPRVSPVDVNP-RVNNP 64

Query: 66  SVVPISDVSSIPIVYPIAPRVSRFNRLSNGAVPRSPVKSQRSSSVPRVQLELHSGERSKF 125
             +P+S                   R++ G    S      S SV  V   LH+   S  
Sbjct: 65  IPLPVS-------------------RIAGGVTSSSGGSPASSESVVSV---LHNNPESSS 124

Query: 126 GNGVDEVFSSELSAQHLNSESEPAIVEGKLANTVTFVTPRDSEDDEEDVFSSPRSCTTDV 185
           G+      S     Q+ N    P +V+ K  +    +  R++ ++E++   +       V
Sbjct: 125 GSASVSPVSGH--RQNGNQVRRP-VVKFKPVDDHDRIEGREAAEEEDNNVEAETERERKV 184

Query: 186 MTSPALSHSRDKRAKKKGICSRCGRGNRLKEREACLVCDAQYCSNCLLKTMGSMPEGRKC 245
               A +  R K  KKK  C RCG+  + + +E C+VCD +YC NC+L+ MGSMPEGRKC
Sbjct: 185 HECTASTKRRKK--KKKSECYRCGKA-KWENKETCIVCDEKYCGNCVLRAMGSMPEGRKC 244

Query: 246 VGCIGRPIDELKRAVLGKCSRILSRVCSPLEIKQIMKAEKECPANQLRPEQLIVNERQLR 305
           V CIG+ IDE KR+ LGK SR+LSR+ SPLE+KQIMKAEKEC ANQLRPEQLIVN   L+
Sbjct: 245 VSCIGQAIDESKRSKLGKHSRVLSRLLSPLEVKQIMKAEKECTANQLRPEQLIVNGYPLK 304

Query: 306 PEELAEILGCAIPPQKLKPGRYWYDKDSGLWGKEGEKPDRIISSKLNVGGKLRADASKGS 365
           PEE+A++L C +PPQKLKPGRYWYDK+SGLWGKEGEKPDR+ISS LN  GKL  DAS G+
Sbjct: 305 PEEMADLLNCLLPPQKLKPGRYWYDKESGLWGKEGEKPDRVISSNLNFTGKLSPDASNGN 364

Query: 366 TKVFINGREITKVELRVLKLANVQCPRDTHFWVYDDGSYEEEGQNNIKGNIWGKASTRLL 425
           T+V+INGREITK+ELR+LKLANVQCPRDTHFWVYDDG YEEEGQNNI+GNIW KASTR +
Sbjct: 365 TEVYINGREITKLELRILKLANVQCPRDTHFWVYDDGRYEEEGQNNIRGNIWEKASTRFM 424

Query: 426 CSLFSLPVP---PVNLHAPKDDPTTISGRSVPDYLEHGRIHKLLLFGIEGSGTSTLFKQA 485
           C+LFSLPVP   P     P  +  T     VP+Y+EH +I KLLL GIEGSGTST+FKQA
Sbjct: 425 CALFSLPVPQGQPRGTVQPSSNYAT-----VPNYIEHKKIQKLLLLGIEGSGTSTIFKQA 484

Query: 486 RFLYGNKFSPEELQNIKLMIQSNVYKYLSTLLEGRERFEEEAIIEKKTGAVIEEKLDKKA 545
           +FLYGNKFS EELQ+IKLM+QSN+Y+YLS LL+GRERFEEEA+   +    +E     + 
Sbjct: 485 KFLYGNKFSVEELQDIKLMVQSNMYRYLSILLDGRERFEEEALSHTRGLNAVEGDSGGEE 544

Query: 546 GTAETGVTESKQCLYSINQRFKHFSDWLLNIMATGDLDAFFPAATREYAPIVDEMWKDSA 605
              E G   + Q +Y++N R KHFSDWLL+I+ATGDLDAFFPAATREYAP+V+E+WKD A
Sbjct: 545 ANDE-GTVTTPQSVYTLNPRLKHFSDWLLDIIATGDLDAFFPAATREYAPLVEEVWKDPA 604

Query: 606 LQETYRRRDELHSLPDVTKYFLDRVIEISSNEYEPSDKDILYAEGVSQSNGLAFMEFYFD 665
           +Q TYRR+DELH LPDV +YFL R +E+SSNEYEPS++DI+YAEGV+Q NGLAFMEF   
Sbjct: 605 IQATYRRKDELHFLPDVAEYFLSRAMEVSSNEYEPSERDIVYAEGVTQGNGLAFMEFSLS 664

Query: 666 DRSPVSELYGENLE-LPPPLTKYQLIQINSEGLVDGCKWLDMFEDVRAMIFCVSLSDYDQ 725
           D SP+SE Y EN + L  P  KYQLI++N++G+ D CKW++MFEDVRA+IFC+SLSDYDQ
Sbjct: 665 DHSPMSESYPENPDALSSPQPKYQLIRVNAKGMNDSCKWVEMFEDVRAVIFCISLSDYDQ 724

Query: 726 --MLSHSKGPL--QNKMLASRNLFEHLVRHPCFGNTPFLLLLNKYDAFEEKINQVPLSSC 785
             +   S G +  QNKM+ S+ LFE +V+HPCF +TPF+L+LNKYD FEEK+N+ PL+SC
Sbjct: 725 INITPESSGTVQYQNKMIQSKELFESMVKHPCFKDTPFILILNKYDQFEEKLNRAPLTSC 784

Query: 786 EWFQDFCPVKPHNNSQTLAQQAYYYIAVKFKELYFSISGQKLFVWHTRARERASVDEAFR 845
           +WF DFCPV+ +NN Q+LA QAY+Y+A+KFK LYFSI+GQKLFVW  RAR+RA+VDE F+
Sbjct: 785 DWFSDFCPVRTNNNVQSLAYQAYFYVAMKFKLLYFSITGQKLFVWQARARDRANVDEGFK 843

Query: 846 YIREVLKWEEEKNDSMYDIVGDESFYSTEISSSP 871
           Y+REVLKW+EEK +S  +  G++SFYST++SSSP
Sbjct: 845 YVREVLKWDEEKEESYLNGGGEDSFYSTDMSSSP 843

BLAST of Cucsa.112140 vs. TAIR10
Match: AT2G23460.1 (AT2G23460.1 extra-large G-protein 1)

HSP 1 Score: 713.4 bits (1840), Expect = 1.7e-205
Identity = 372/727 (51.17%), Postives = 492/727 (67.68%), Query Frame = 1

Query: 151 EGKLANTVTF--VTPRDSEDDEEDVFSSPRSCTTDVMTSPALSHSRDKRAKKKGICSRCG 210
           +GK    VTF  +   D  ++EE   +  R     V   P       +   KKG C RC 
Sbjct: 177 DGKKQPVVTFLGIASDDGFEEEESCSNLRRVRVVPVKKQP-------QTKGKKGSCYRCF 236

Query: 211 RGNRLKEREACLVCDAQYCSNCLLKTMGSMPEGRKCVGCIGRPIDELKRAVLGKCSRILS 270
           +G+R  E+E CLVCDA+YC++C+L+ MGSMPEGRKCV CIG PIDE KR  LGKCSR+L 
Sbjct: 237 KGSRFTEKEVCLVCDAKYCNSCVLRAMGSMPEGRKCVTCIGFPIDESKRGSLGKCSRMLK 296

Query: 271 RVCSPLEIKQIMKAEKECPANQLRPEQLIVNERQLRPEELAEILGCAIPPQKLKPGRYWY 330
           R+ + LE+KQIMK E+ C ANQL  E + VN + L PEEL  +  C+ PP+KLKPG YWY
Sbjct: 297 RLLNDLEVKQIMKTERFCEANQLPAEYVYVNGQPLYPEELVTLQTCSNPPKKLKPGDYWY 356

Query: 331 DKDSGLWGKEGEKPDRIISSKLNVGGKLRADASKGSTKVFINGREITKVELRVLKLANVQ 390
           DK SGLWGKEGEKP +IIS  LNVGG +  +AS G+T+VFINGREITKVELR+L+LA VQ
Sbjct: 357 DKVSGLWGKEGEKPYQIISPHLNVGGPISPEASNGNTQVFINGREITKVELRMLQLAGVQ 416

Query: 391 CPRDTHFWVYDDGSYEEEGQNNIKGNIWGKASTRLLCSLFSLPVPPVN-LHAPKDDPTTI 450
           C  + HFWV +DGSY+EEGQ N KG IWGKA T+LLC++ SLPVP  +  +A  +   + 
Sbjct: 417 CAGNPHFWVNEDGSYQEEGQKNTKGYIWGKAGTKLLCAVLSLPVPSKSTANASGEQLYSA 476

Query: 451 SGRSVPDYLEHGRIHKLLLFGIEGSGTSTLFKQARFLYGN-KFSPEELQNIKLMIQSNVY 510
           + RS+ D+LEH  + K+LL G  GSGTST+FKQA+ LY +  F  +E +NIK++IQ+NVY
Sbjct: 477 NSRSILDHLEHRTLQKILLVGNSGSGTSTIFKQAKILYKDVPFLEDERENIKVIIQTNVY 536

Query: 511 KYLSTLLEGRERFEEEAIIEKKTGAVIEEKLDKKAGTAETGVTESKQCLYSINQRFKHFS 570
            YL  LLEGRERFEEEA+  + T   + E +    G A++   +    +YSI  R K FS
Sbjct: 537 GYLGMLLEGRERFEEEALALRNTKQCVLENIPADEGDAKS--NDKTVTMYSIGPRLKAFS 596

Query: 571 DWLLNIMATGDLDAFFPAATREYAPIVDEMWKDSALQETYRRRDELHSLPDVTKYFLDRV 630
           DWLL  MA G+L   FPAA+REYAP+V+E+W+D+A+Q TY+RR EL  LP V  YFL+R 
Sbjct: 597 DWLLKTMAAGNLGVIFPAASREYAPLVEELWRDAAIQATYKRRSELGLLPSVASYFLERA 656

Query: 631 IEISSNEYEPSDKDILYAEGVSQSNGLAFMEFYFDDRSPVSELYGENLELPPPLTKYQLI 690
           I++ + +YEPSD DILYAEGV+ S+GLA ++F F   +    L  +  +    L +YQLI
Sbjct: 657 IDVLTPDYEPSDLDILYAEGVTSSSGLACLDFSFPQTASEENL--DPSDHHDSLLRYQLI 716

Query: 691 QINSEGLVDGCKWLDMFEDVRAMIFCVSLSDYDQMLSHSKGPLQNKMLASRNLFEHLVRH 750
           ++ S GL + CKW+DMFEDV  ++F VS+SDYDQ+         NKML ++ LFE ++ H
Sbjct: 717 RVPSRGLGENCKWIDMFEDVGMVVFVVSMSDYDQVSEDG----TNKMLLTKKLFESIITH 776

Query: 751 PCFGNTPFLLLLNKYDAFEEKINQVPLSSCEWFQDFCPV-----KPHNNSQTLAQQAYYY 810
           P F N  FLL+LNKYD  EEK+ +VPL+ CEWFQDF PV       +N + TL Q A+++
Sbjct: 777 PIFENMDFLLILNKYDLLEEKVERVPLARCEWFQDFNPVVSRHRGSNNGNPTLGQLAFHF 836

Query: 811 IAVKFKELYFSISGQKLFVWHTRARERASVDEAFRYIREVLKWEEEKNDSMYDIVGDESF 869
           +AVKFK  Y S++G+KLFV  +++ +  SVD + +   E+LKW EE+ +     + + S 
Sbjct: 837 MAVKFKRFYSSLTGKKLFVSSSKSLDPNSVDSSLKLAMEILKWSEERTNI---CMSEYSM 885

HSP 2 Score: 37.7 bits (86), Expect = 4.3e-02
Identity = 18/47 (38.30%), Postives = 30/47 (63.83%), Query Frame = 1

Query: 36 AVEYQGPPVAHELPKIDPLDLESLSILS-PSVVPISDVSSIPIVYPI 82
          A EY GPP+++ +P   P+++E + + +  S V ISD  S P++ PI
Sbjct: 12 AEEYDGPPLSYNIPCAVPINVEKIPVAAVVSPVCISDNMSFPVIQPI 58

BLAST of Cucsa.112140 vs. TAIR10
Match: AT4G34390.1 (AT4G34390.1 extra-large GTP-binding protein 2)

HSP 1 Score: 595.5 bits (1534), Expect = 5.3e-170
Identity = 368/905 (40.66%), Postives = 518/905 (57.24%), Query Frame = 1

Query: 15  IRRMLPAGAPLPDEEH--------------LDYSIAVEYQGPPVAHELPKIDPLDLESLS 74
           IR++LP  +P P  ++              ++YS A EY+GP +A+ +P+  P++++ + 
Sbjct: 5   IRKLLPFPSPNPKRDNRESDDDDETSSGYRIEYSFASEYKGPLIAN-VPRALPVEVDQI- 64

Query: 75  ILSPSVVPISDVS-SIPIVYPIAPRVSRFN--RLSNGAVPRSP--VKSQRSSSVPRVQLE 134
              P+ +P+S  S    I YP+AP V   +  R  +  + +    V S   SSV  +  +
Sbjct: 65  ---PTALPVSFSSLRSGISYPVAPLVMTKDTKRPPDSGIEKKNGFVDSAAGSSVVLIGRD 124

Query: 135 LHSGERSKFGNG----VDEVFS------SELSAQHLNSESEPAIVEGKLANT----VTFV 194
           + SG  S   +      +EV S      S  S    ++  E  + + ++++     V FV
Sbjct: 125 VVSGSSSSSSSKRLDVPEEVKSPADFRLSPSSPLSASAREEDHLDDDRVSDVGPRAVRFV 184

Query: 195 TPRDSEDDEEDVFSSPRSCTTDVMTSPALSHSRDKRAKKKGICSRCGRGNRLKEREACLV 254
            P  S + +E  + S          S A +H R +R  K+G C RC  GNR  E+E C+V
Sbjct: 185 EPFQSSECDESSYVSDGE-------SIAATH-RAERKGKRGSCYRCQLGNRFTEKEVCIV 244

Query: 255 CDAQYCSNCLLKTMGSMPEGRKCVGCIGRPIDELKRAVLGKCSRILSRVCSPLEIKQIMK 314
           CDA+YC NC+ + MG+MPEGRKC  CIG  IDE KRA LGKCSR+L R  +  E++Q+M 
Sbjct: 245 CDAKYCFNCVRRAMGAMPEGRKCQACIGYRIDESKRASLGKCSRMLKRHLTDSELRQVMN 304

Query: 315 AEKECPANQLRPEQLIVNERQLRPEELAEILGCAIPPQKLKPGRYWYDKDSGLWGKEGEK 374
           AE  C ANQL    +IVN++ L  +EL  +  C  PP+KLKPG YWYDK +G WGK GEK
Sbjct: 305 AEITCKANQLPSRLIIVNDKPLSEDELYTLQTCPNPPKKLKPGHYWYDKVAGYWGKIGEK 364

Query: 375 PDRIISSKLNVGGKLRADASKGSTKVFINGREITKVELRVLKLANVQCPRDTHFWVYDDG 434
           P +IIS   ++GG +    S G T+++INGREITK EL +LK A VQC    HFWV  DG
Sbjct: 365 PSQIISPNNSIGGYISEKVSNGDTEIYINGREITKPELTMLKWAGVQCEGKPHFWVDSDG 424

Query: 435 SYEEEGQNNIKGNIWGKASTRLLCSLFSLPVPPVNLHAPKDDPTTISGRSVPDYLEHGRI 494
           SY EEGQ +  GNIW K   ++ C++FSLPVPP +        + +    VP Y E   +
Sbjct: 425 SYREEGQKHPIGNIWSKKRAKIACAVFSLPVPPAS--------SAVEPYDVPLY-EQKML 484

Query: 495 HKLLLFGIEGSGTSTLFKQARFLYGNKFSPEELQNIKLMIQSNVYKYLSTLLEGRERFEE 554
           +KLLL G E  G +T++KQAR LY   FS E+ + IK +IQ+N+Y YL+ +LE  ERFE+
Sbjct: 485 NKLLLIGSEKGGATTIYKQARSLYNVSFSLEDRERIKFIIQTNLYTYLAMVLEAHERFEK 544

Query: 555 EAIIEKKTGAVIEEKLDKKAGTAETGVTESKQCLYSINQRFKHFSDWLLNIMATGDLDAF 614
           E   ++ +G V +E   K                 SIN R KHFSDW+L     G+L   
Sbjct: 545 EMSNDQSSGNVGDETSAKPGN--------------SINPRLKHFSDWVLKEKEDGNL-KI 604

Query: 615 FPAATREYAPIVDEMWKDSALQETYRRRDELHSLPDVTKYFLDRVIEISSNEYEPSDKDI 674
           FP ++RE A  V ++W+  A+Q TY+R  +  +LP    YFL+R++EIS +EY+PSD DI
Sbjct: 605 FPPSSRENAQTVADLWRVPAIQATYKRLRD--TLPRNAVYFLERILEISRSEYDPSDMDI 664

Query: 675 LYAEGVSQSNGLAFMEFYFDDRSPVSEL---YGENLELPPPLTKYQLIQINSEGLVDGCK 734
           L AEG+S   GL+ ++F F   S    L   Y  + ++     KYQLI++N   L +  K
Sbjct: 665 LQAEGLSSMEGLSCVDFSFPSTSQEESLESDYQHDTDM-----KYQLIRLNPRSLGENWK 724

Query: 735 WLDMFEDVRAMIFCVSLSDYDQMLSHSKGPLQNKMLASRNLFEHLVRHPCFGNTPFLLLL 794
            L+MFED   +IFCVSL+DY + +   +G + NKMLA++ LFE++V HP   N  FLL+L
Sbjct: 725 LLEMFEDADLVIFCVSLTDYAENIEDGEGNIVNKMLATKQLFENMVTHPSLANKRFLLVL 784

Query: 795 NKYDAFEEKINQVPLSSCEWFQDFCPVKPHN----NSQTLAQQAYYYIAVKFKELYFSI- 854
            K+D  EEKI +VPL +CEWF+DF P+   N    ++  +AQ+A++YI  KFK LY SI 
Sbjct: 785 TKFDLLEEKIEEVPLRTCEWFEDFNPLISQNQTSRHNPPMAQRAFHYIGYKFKRLYDSIL 844

Query: 855 ---------SGQKLFVWHTRARERASVDEAFRYIREVLKWEEEKNDSMYDIVGDESFYST 870
                       KLFV    + E  +VD A RY RE+LKW  E+  SM+    + S  S 
Sbjct: 845 EPVNMRGRSFKPKLFVCQV-SLESDTVDNALRYAREILKWHVEET-SMFQ---EMSTTSI 860

BLAST of Cucsa.112140 vs. TAIR10
Match: AT2G26300.1 (AT2G26300.1 G protein alpha subunit 1)

HSP 1 Score: 181.4 bits (459), Expect = 2.4e-45
Identity = 121/388 (31.19%), Postives = 205/388 (52.84%), Query Frame = 1

Query: 461 IHKLLLFGIEGSGTSTLFKQARFLYGNKFSPEELQNIKLMIQSNVYKYLSTLLEGRERFE 520
           I KLLL G   SG ST+FKQ + L+   F   EL++   +I +NVY+ +  L +G + F 
Sbjct: 38  IRKLLLLGAGESGKSTIFKQIKLLFQTGFDEGELKSYVPVIHANVYQTIKLLHDGTKEFA 97

Query: 521 EEAIIEKKTGAVIEEKLDKKAGTAETGVTESKQCLYSINQRFKHFSDWLLNIMATGDLDA 580
           +                            E+    Y ++       + L  I   G LD 
Sbjct: 98  QN---------------------------ETDSAKYMLSSESIAIGEKLSEI--GGRLD- 157

Query: 581 FFPAATREYAPIVDEMWKDSALQETYRRRDELHSLPDVTKYFLDRVIEISSNEYEPSDKD 640
            +P  T++ A  ++ +WKD A+QET  R +EL  +PD TKY ++ +  +S   Y P+ +D
Sbjct: 158 -YPRLTKDIAEGIETLWKDPAIQETCARGNELQ-VPDCTKYLMENLKRLSDINYIPTKED 217

Query: 641 ILYAEGVSQSNGLAFMEFYFDDRSPVSELYGENLELPPPLTKYQLIQINSEGLVDGCKWL 700
           +LYA    ++ G+  ++F     SPV    GEN +       Y+L  +  +   +  KW+
Sbjct: 218 VLYAR--VRTTGVVEIQF-----SPV----GENKKSGEV---YRLFDVGGQRN-ERRKWI 277

Query: 701 DMFEDVRAMIFCVSLSDYDQMLSHSKGPLQNKMLASRNLFEHLVRHPCFGNTPFLLLLNK 760
            +FE V A+IFC ++S+YDQ L   +   +N+M+ ++ LF+ +++ PCF  T F+L LNK
Sbjct: 278 HLFEGVTAVIFCAAISEYDQTLFEDEQ--KNRMMETKELFDWVLKQPCFEKTSFMLFLNK 337

Query: 761 YDAFEEKINQVPLSSCEWFQDFCPVKPHNNSQTLAQQAYYYIAVKFKELYFSISG----Q 820
           +D FE+K+  VPL+ CEWF+D+ PV   ++ +   + AY ++  KF+ELY+  +      
Sbjct: 338 FDIFEKKVLDVPLNVCEWFRDYQPV---SSGKQEIEHAYEFVKKKFEELYYQNTAPDRVD 373

Query: 821 KLF-VWHTRARERASVDEAFRYIREVLK 844
           ++F ++ T A ++  V + F+ + E L+
Sbjct: 398 RVFKIYRTTALDQKLVKKTFKLVDETLR 373

BLAST of Cucsa.112140 vs. TAIR10
Match: AT2G46380.1 (AT2G46380.1 Protein of unknown function (DUF3133))

HSP 1 Score: 54.3 bits (129), Expect = 4.4e-07
Identity = 28/87 (32.18%), Postives = 47/87 (54.02%), Query Frame = 1

Query: 321 LKPGRYWYDKDSGLWGKEGEKPDRIISSKLN-VGGKLRADASKGSTKVFINGREITKVEL 380
           ++PG YWYD  +G WG  G     I+   +  +   +  + + G+T+VF+NGRE+ + +L
Sbjct: 650 IRPGNYWYDYRAGFWGVLGSHCLGILPPFIEELNYPMPENCAGGTTRVFVNGRELHQKDL 709

Query: 381 RVLKLANVQCPRDTHFWVYDDGSYEEE 407
           R+L    +   RD  + VY  G   +E
Sbjct: 710 RLLTARGLPRDRDRSYTVYISGRVIDE 736

BLAST of Cucsa.112140 vs. NCBI nr
Match: gi|778656685|ref|XP_011649510.1| (PREDICTED: extra-large guanine nucleotide-binding protein 3-like isoform X1 [Cucumis sativus])

HSP 1 Score: 1780.0 bits (4609), Expect = 0.0e+00
Identity = 878/878 (100.00%), Postives = 878/878 (100.00%), Query Frame = 1

Query: 1   MASDTDEENAWQEAIRRMLPAGAPLPDEEHLDYSIAVEYQGPPVAHELPKIDPLDLESLS 60
           MASDTDEENAWQEAIRRMLPAGAPLPDEEHLDYSIAVEYQGPPVAHELPKIDPLDLESLS
Sbjct: 1   MASDTDEENAWQEAIRRMLPAGAPLPDEEHLDYSIAVEYQGPPVAHELPKIDPLDLESLS 60

Query: 61  ILSPSVVPISDVSSIPIVYPIAPRVSRFNRLSNGAVPRSPVKSQRSSSVPRVQLELHSGE 120
           ILSPSVVPISDVSSIPIVYPIAPRVSRFNRLSNGAVPRSPVKSQRSSSVPRVQLELHSGE
Sbjct: 61  ILSPSVVPISDVSSIPIVYPIAPRVSRFNRLSNGAVPRSPVKSQRSSSVPRVQLELHSGE 120

Query: 121 RSKFGNGVDEVFSSELSAQHLNSESEPAIVEGKLANTVTFVTPRDSEDDEEDVFSSPRSC 180
           RSKFGNGVDEVFSSELSAQHLNSESEPAIVEGKLANTVTFVTPRDSEDDEEDVFSSPRSC
Sbjct: 121 RSKFGNGVDEVFSSELSAQHLNSESEPAIVEGKLANTVTFVTPRDSEDDEEDVFSSPRSC 180

Query: 181 TTDVMTSPALSHSRDKRAKKKGICSRCGRGNRLKEREACLVCDAQYCSNCLLKTMGSMPE 240
           TTDVMTSPALSHSRDKRAKKKGICSRCGRGNRLKEREACLVCDAQYCSNCLLKTMGSMPE
Sbjct: 181 TTDVMTSPALSHSRDKRAKKKGICSRCGRGNRLKEREACLVCDAQYCSNCLLKTMGSMPE 240

Query: 241 GRKCVGCIGRPIDELKRAVLGKCSRILSRVCSPLEIKQIMKAEKECPANQLRPEQLIVNE 300
           GRKCVGCIGRPIDELKRAVLGKCSRILSRVCSPLEIKQIMKAEKECPANQLRPEQLIVNE
Sbjct: 241 GRKCVGCIGRPIDELKRAVLGKCSRILSRVCSPLEIKQIMKAEKECPANQLRPEQLIVNE 300

Query: 301 RQLRPEELAEILGCAIPPQKLKPGRYWYDKDSGLWGKEGEKPDRIISSKLNVGGKLRADA 360
           RQLRPEELAEILGCAIPPQKLKPGRYWYDKDSGLWGKEGEKPDRIISSKLNVGGKLRADA
Sbjct: 301 RQLRPEELAEILGCAIPPQKLKPGRYWYDKDSGLWGKEGEKPDRIISSKLNVGGKLRADA 360

Query: 361 SKGSTKVFINGREITKVELRVLKLANVQCPRDTHFWVYDDGSYEEEGQNNIKGNIWGKAS 420
           SKGSTKVFINGREITKVELRVLKLANVQCPRDTHFWVYDDGSYEEEGQNNIKGNIWGKAS
Sbjct: 361 SKGSTKVFINGREITKVELRVLKLANVQCPRDTHFWVYDDGSYEEEGQNNIKGNIWGKAS 420

Query: 421 TRLLCSLFSLPVPPVNLHAPKDDPTTISGRSVPDYLEHGRIHKLLLFGIEGSGTSTLFKQ 480
           TRLLCSLFSLPVPPVNLHAPKDDPTTISGRSVPDYLEHGRIHKLLLFGIEGSGTSTLFKQ
Sbjct: 421 TRLLCSLFSLPVPPVNLHAPKDDPTTISGRSVPDYLEHGRIHKLLLFGIEGSGTSTLFKQ 480

Query: 481 ARFLYGNKFSPEELQNIKLMIQSNVYKYLSTLLEGRERFEEEAIIEKKTGAVIEEKLDKK 540
           ARFLYGNKFSPEELQNIKLMIQSNVYKYLSTLLEGRERFEEEAIIEKKTGAVIEEKLDKK
Sbjct: 481 ARFLYGNKFSPEELQNIKLMIQSNVYKYLSTLLEGRERFEEEAIIEKKTGAVIEEKLDKK 540

Query: 541 AGTAETGVTESKQCLYSINQRFKHFSDWLLNIMATGDLDAFFPAATREYAPIVDEMWKDS 600
           AGTAETGVTESKQCLYSINQRFKHFSDWLLNIMATGDLDAFFPAATREYAPIVDEMWKDS
Sbjct: 541 AGTAETGVTESKQCLYSINQRFKHFSDWLLNIMATGDLDAFFPAATREYAPIVDEMWKDS 600

Query: 601 ALQETYRRRDELHSLPDVTKYFLDRVIEISSNEYEPSDKDILYAEGVSQSNGLAFMEFYF 660
           ALQETYRRRDELHSLPDVTKYFLDRVIEISSNEYEPSDKDILYAEGVSQSNGLAFMEFYF
Sbjct: 601 ALQETYRRRDELHSLPDVTKYFLDRVIEISSNEYEPSDKDILYAEGVSQSNGLAFMEFYF 660

Query: 661 DDRSPVSELYGENLELPPPLTKYQLIQINSEGLVDGCKWLDMFEDVRAMIFCVSLSDYDQ 720
           DDRSPVSELYGENLELPPPLTKYQLIQINSEGLVDGCKWLDMFEDVRAMIFCVSLSDYDQ
Sbjct: 661 DDRSPVSELYGENLELPPPLTKYQLIQINSEGLVDGCKWLDMFEDVRAMIFCVSLSDYDQ 720

Query: 721 MLSHSKGPLQNKMLASRNLFEHLVRHPCFGNTPFLLLLNKYDAFEEKINQVPLSSCEWFQ 780
           MLSHSKGPLQNKMLASRNLFEHLVRHPCFGNTPFLLLLNKYDAFEEKINQVPLSSCEWFQ
Sbjct: 721 MLSHSKGPLQNKMLASRNLFEHLVRHPCFGNTPFLLLLNKYDAFEEKINQVPLSSCEWFQ 780

Query: 781 DFCPVKPHNNSQTLAQQAYYYIAVKFKELYFSISGQKLFVWHTRARERASVDEAFRYIRE 840
           DFCPVKPHNNSQTLAQQAYYYIAVKFKELYFSISGQKLFVWHTRARERASVDEAFRYIRE
Sbjct: 781 DFCPVKPHNNSQTLAQQAYYYIAVKFKELYFSISGQKLFVWHTRARERASVDEAFRYIRE 840

Query: 841 VLKWEEEKNDSMYDIVGDESFYSTEISSSPGMNNLPVT 879
           VLKWEEEKNDSMYDIVGDESFYSTEISSSPGMNNLPVT
Sbjct: 841 VLKWEEEKNDSMYDIVGDESFYSTEISSSPGMNNLPVT 878

BLAST of Cucsa.112140 vs. NCBI nr
Match: gi|659066231|ref|XP_008444133.1| (PREDICTED: extra-large guanine nucleotide-binding protein 3-like isoform X1 [Cucumis melo])

HSP 1 Score: 1754.6 bits (4543), Expect = 0.0e+00
Identity = 862/878 (98.18%), Postives = 871/878 (99.20%), Query Frame = 1

Query: 1   MASDTDEENAWQEAIRRMLPAGAPLPDEEHLDYSIAVEYQGPPVAHELPKIDPLDLESLS 60
           MASDT+EENAWQEA+RRMLPAGAPLPDEEHLDYSIAVEYQGPPVAHELPKIDPLDLESLS
Sbjct: 1   MASDTNEENAWQEALRRMLPAGAPLPDEEHLDYSIAVEYQGPPVAHELPKIDPLDLESLS 60

Query: 61  ILSPSVVPISDVSSIPIVYPIAPRVSRFNRLSNGAVPRSPVKSQRSSSVPRVQLELHSGE 120
           ILSPSVVPISD+SSIPIVYPIAPRVSRFNRLSNGAVPRSPVKSQRSSSVPRVQLE H+GE
Sbjct: 61  ILSPSVVPISDISSIPIVYPIAPRVSRFNRLSNGAVPRSPVKSQRSSSVPRVQLESHNGE 120

Query: 121 RSKFGNGVDEVFSSELSAQHLNSESEPAIVEGKLANTVTFVTPRDSEDDEEDVFSSPRSC 180
           RSKFGNGVDE FSSELSAQHLNSESEPAI EGK ANTVTFVTPRDSEDDEEDVFSSPRSC
Sbjct: 121 RSKFGNGVDEAFSSELSAQHLNSESEPAIAEGKRANTVTFVTPRDSEDDEEDVFSSPRSC 180

Query: 181 TTDVMTSPALSHSRDKRAKKKGICSRCGRGNRLKEREACLVCDAQYCSNCLLKTMGSMPE 240
           TTDVMTSPALSHSR+KRAKKKGICSRCGRGNRLKEREACLVCDAQYCSNCLLK MGSMPE
Sbjct: 181 TTDVMTSPALSHSREKRAKKKGICSRCGRGNRLKEREACLVCDAQYCSNCLLKAMGSMPE 240

Query: 241 GRKCVGCIGRPIDELKRAVLGKCSRILSRVCSPLEIKQIMKAEKECPANQLRPEQLIVNE 300
           GRKCVGCIGRPIDELKRAVLGKCSRILSRVCSPLEIKQIMKAEKECPANQLRPEQLIVNE
Sbjct: 241 GRKCVGCIGRPIDELKRAVLGKCSRILSRVCSPLEIKQIMKAEKECPANQLRPEQLIVNE 300

Query: 301 RQLRPEELAEILGCAIPPQKLKPGRYWYDKDSGLWGKEGEKPDRIISSKLNVGGKLRADA 360
           RQLRPEELAEILGCAIPPQKLKPGRYWYDKDSGLWGKEGEKPDRIISSKLNVGGKLRADA
Sbjct: 301 RQLRPEELAEILGCAIPPQKLKPGRYWYDKDSGLWGKEGEKPDRIISSKLNVGGKLRADA 360

Query: 361 SKGSTKVFINGREITKVELRVLKLANVQCPRDTHFWVYDDGSYEEEGQNNIKGNIWGKAS 420
           SKGSTKVFINGREITKVELRVLKLANVQCPRDTHFWVYDDGSYEEEGQNNIKGNIWGKAS
Sbjct: 361 SKGSTKVFINGREITKVELRVLKLANVQCPRDTHFWVYDDGSYEEEGQNNIKGNIWGKAS 420

Query: 421 TRLLCSLFSLPVPPVNLHAPKDDPTTISGRSVPDYLEHGRIHKLLLFGIEGSGTSTLFKQ 480
           TRLLCSLFSLPVPPVNLHAPK+DPTTISGRSVPDYLEHGRIHKLLLFGIEGSGTSTLFKQ
Sbjct: 421 TRLLCSLFSLPVPPVNLHAPKEDPTTISGRSVPDYLEHGRIHKLLLFGIEGSGTSTLFKQ 480

Query: 481 ARFLYGNKFSPEELQNIKLMIQSNVYKYLSTLLEGRERFEEEAIIEKKTGAVIEEKLDKK 540
           ARFLYGNKF+PEELQNIKLMIQSNVYKYLSTLLEGRERFEEEAIIEKKTGAVIEEKLDKK
Sbjct: 481 ARFLYGNKFTPEELQNIKLMIQSNVYKYLSTLLEGRERFEEEAIIEKKTGAVIEEKLDKK 540

Query: 541 AGTAETGVTESKQCLYSINQRFKHFSDWLLNIMATGDLDAFFPAATREYAPIVDEMWKDS 600
           AGTAETGVTESKQCLYSINQRFKHFSDWLLNIMATGDLDAFFPAATREYAPIVDEMWKDS
Sbjct: 541 AGTAETGVTESKQCLYSINQRFKHFSDWLLNIMATGDLDAFFPAATREYAPIVDEMWKDS 600

Query: 601 ALQETYRRRDELHSLPDVTKYFLDRVIEISSNEYEPSDKDILYAEGVSQSNGLAFMEFYF 660
           ALQETYRRRDELHSLPDVTKYFLDRVIEISSNEYEPSDKDILYAEGVSQSNGLAFMEFYF
Sbjct: 601 ALQETYRRRDELHSLPDVTKYFLDRVIEISSNEYEPSDKDILYAEGVSQSNGLAFMEFYF 660

Query: 661 DDRSPVSELYGENLELPPPLTKYQLIQINSEGLVDGCKWLDMFEDVRAMIFCVSLSDYDQ 720
           DDRSPVSELYGENLEL PPLTKYQLIQINSEGLVDGCKWLDMFEDVRAMIFCVSLSDYDQ
Sbjct: 661 DDRSPVSELYGENLELAPPLTKYQLIQINSEGLVDGCKWLDMFEDVRAMIFCVSLSDYDQ 720

Query: 721 MLSHSKGPLQNKMLASRNLFEHLVRHPCFGNTPFLLLLNKYDAFEEKINQVPLSSCEWFQ 780
           MLSHSKGPLQNKMLASRNLFEHLVRHPCFGNTPFLLLLNKYDAFEEKINQVPLSSCEWFQ
Sbjct: 721 MLSHSKGPLQNKMLASRNLFEHLVRHPCFGNTPFLLLLNKYDAFEEKINQVPLSSCEWFQ 780

Query: 781 DFCPVKPHNNSQTLAQQAYYYIAVKFKELYFSISGQKLFVWHTRARERASVDEAFRYIRE 840
           DFCPVKPHNN+QTLAQQAYYYIAVKFKELYFSISGQKLFVWHTRARERASVDEAFRYIRE
Sbjct: 781 DFCPVKPHNNNQTLAQQAYYYIAVKFKELYFSISGQKLFVWHTRARERASVDEAFRYIRE 840

Query: 841 VLKWEEEKNDSMYDIVGDESFYSTEISSSPGMNNLPVT 879
           VLKWEEEKNDSMYDIVGDESFYSTEISSSPG+NN PVT
Sbjct: 841 VLKWEEEKNDSMYDIVGDESFYSTEISSSPGVNNFPVT 878

BLAST of Cucsa.112140 vs. NCBI nr
Match: gi|778656688|ref|XP_011649515.1| (PREDICTED: extra-large guanine nucleotide-binding protein 3-like isoform X2 [Cucumis sativus])

HSP 1 Score: 1352.0 bits (3498), Expect = 0.0e+00
Identity = 671/673 (99.70%), Postives = 671/673 (99.70%), Query Frame = 1

Query: 1   MASDTDEENAWQEAIRRMLPAGAPLPDEEHLDYSIAVEYQGPPVAHELPKIDPLDLESLS 60
           MASDTDEENAWQEAIRRMLPAGAPLPDEEHLDYSIAVEYQGPPVAHELPKIDPLDLESLS
Sbjct: 1   MASDTDEENAWQEAIRRMLPAGAPLPDEEHLDYSIAVEYQGPPVAHELPKIDPLDLESLS 60

Query: 61  ILSPSVVPISDVSSIPIVYPIAPRVSRFNRLSNGAVPRSPVKSQRSSSVPRVQLELHSGE 120
           ILSPSVVPISDVSSIPIVYPIAPRVSRFNRLSNGAVPRSPVKSQRSSSVPRVQLELHSGE
Sbjct: 61  ILSPSVVPISDVSSIPIVYPIAPRVSRFNRLSNGAVPRSPVKSQRSSSVPRVQLELHSGE 120

Query: 121 RSKFGNGVDEVFSSELSAQHLNSESEPAIVEGKLANTVTFVTPRDSEDDEEDVFSSPRSC 180
           RSKFGNGVDEVFSSELSAQHLNSESEPAIVEGKLANTVTFVTPRDSEDDEEDVFSSPRSC
Sbjct: 121 RSKFGNGVDEVFSSELSAQHLNSESEPAIVEGKLANTVTFVTPRDSEDDEEDVFSSPRSC 180

Query: 181 TTDVMTSPALSHSRDKRAKKKGICSRCGRGNRLKEREACLVCDAQYCSNCLLKTMGSMPE 240
           TTDVMTSPALSHSRDKRAKKKGICSRCGRGNRLKEREACLVCDAQYCSNCLLKTMGSMPE
Sbjct: 181 TTDVMTSPALSHSRDKRAKKKGICSRCGRGNRLKEREACLVCDAQYCSNCLLKTMGSMPE 240

Query: 241 GRKCVGCIGRPIDELKRAVLGKCSRILSRVCSPLEIKQIMKAEKECPANQLRPEQLIVNE 300
           GRKCVGCIGRPIDELKRAVLGKCSRILSRVCSPLEIKQIMKAEKECPANQLRPEQLIVNE
Sbjct: 241 GRKCVGCIGRPIDELKRAVLGKCSRILSRVCSPLEIKQIMKAEKECPANQLRPEQLIVNE 300

Query: 301 RQLRPEELAEILGCAIPPQKLKPGRYWYDKDSGLWGKEGEKPDRIISSKLNVGGKLRADA 360
           RQLRPEELAEILGCAIPPQKLKPGRYWYDKDSGLWGKEGEKPDRIISSKLNVGGKLRADA
Sbjct: 301 RQLRPEELAEILGCAIPPQKLKPGRYWYDKDSGLWGKEGEKPDRIISSKLNVGGKLRADA 360

Query: 361 SKGSTKVFINGREITKVELRVLKLANVQCPRDTHFWVYDDGSYEEEGQNNIKGNIWGKAS 420
           SKGSTKVFINGREITKVELRVLKLANVQCPRDTHFWVYDDGSYEEEGQNNIKGNIWGKAS
Sbjct: 361 SKGSTKVFINGREITKVELRVLKLANVQCPRDTHFWVYDDGSYEEEGQNNIKGNIWGKAS 420

Query: 421 TRLLCSLFSLPVPPVNLHAPKDDPTTISGRSVPDYLEHGRIHKLLLFGIEGSGTSTLFKQ 480
           TRLLCSLFSLPVPPVNLHAPKDDPTTISGRSVPDYLEHGRIHKLLLFGIEGSGTSTLFKQ
Sbjct: 421 TRLLCSLFSLPVPPVNLHAPKDDPTTISGRSVPDYLEHGRIHKLLLFGIEGSGTSTLFKQ 480

Query: 481 ARFLYGNKFSPEELQNIKLMIQSNVYKYLSTLLEGRERFEEEAIIEKKTGAVIEEKLDKK 540
           ARFLYGNKFSPEELQNIKLMIQSNVYKYLSTLLEGRERFEEEAIIEKKTGAVIEEKLDKK
Sbjct: 481 ARFLYGNKFSPEELQNIKLMIQSNVYKYLSTLLEGRERFEEEAIIEKKTGAVIEEKLDKK 540

Query: 541 AGTAETGVTESKQCLYSINQRFKHFSDWLLNIMATGDLDAFFPAATREYAPIVDEMWKDS 600
           AGTAETGVTESKQCLYSINQRFKHFSDWLLNIMATGDLDAFFPAATREYAPIVDEMWKDS
Sbjct: 541 AGTAETGVTESKQCLYSINQRFKHFSDWLLNIMATGDLDAFFPAATREYAPIVDEMWKDS 600

Query: 601 ALQETYRRRDELHSLPDVTKYFLDRVIEISSNEYEPSDKDILYAEGVSQSNGLAFMEFYF 660
           ALQETYRRRDELHSLPDVTKYFLDRVIEISSNEYEPSDKDILYAEGVSQSNGLAFMEFYF
Sbjct: 601 ALQETYRRRDELHSLPDVTKYFLDRVIEISSNEYEPSDKDILYAEGVSQSNGLAFMEFYF 660

Query: 661 DDRSPVSELYGEN 674
           DDRSPVSELY  N
Sbjct: 661 DDRSPVSELYDTN 673

BLAST of Cucsa.112140 vs. NCBI nr
Match: gi|659066233|ref|XP_008444885.1| (PREDICTED: extra-large guanine nucleotide-binding protein 3-like isoform X2 [Cucumis melo])

HSP 1 Score: 1333.9 bits (3451), Expect = 0.0e+00
Identity = 659/673 (97.92%), Postives = 666/673 (98.96%), Query Frame = 1

Query: 1   MASDTDEENAWQEAIRRMLPAGAPLPDEEHLDYSIAVEYQGPPVAHELPKIDPLDLESLS 60
           MASDT+EENAWQEA+RRMLPAGAPLPDEEHLDYSIAVEYQGPPVAHELPKIDPLDLESLS
Sbjct: 1   MASDTNEENAWQEALRRMLPAGAPLPDEEHLDYSIAVEYQGPPVAHELPKIDPLDLESLS 60

Query: 61  ILSPSVVPISDVSSIPIVYPIAPRVSRFNRLSNGAVPRSPVKSQRSSSVPRVQLELHSGE 120
           ILSPSVVPISD+SSIPIVYPIAPRVSRFNRLSNGAVPRSPVKSQRSSSVPRVQLE H+GE
Sbjct: 61  ILSPSVVPISDISSIPIVYPIAPRVSRFNRLSNGAVPRSPVKSQRSSSVPRVQLESHNGE 120

Query: 121 RSKFGNGVDEVFSSELSAQHLNSESEPAIVEGKLANTVTFVTPRDSEDDEEDVFSSPRSC 180
           RSKFGNGVDE FSSELSAQHLNSESEPAI EGK ANTVTFVTPRDSEDDEEDVFSSPRSC
Sbjct: 121 RSKFGNGVDEAFSSELSAQHLNSESEPAIAEGKRANTVTFVTPRDSEDDEEDVFSSPRSC 180

Query: 181 TTDVMTSPALSHSRDKRAKKKGICSRCGRGNRLKEREACLVCDAQYCSNCLLKTMGSMPE 240
           TTDVMTSPALSHSR+KRAKKKGICSRCGRGNRLKEREACLVCDAQYCSNCLLK MGSMPE
Sbjct: 181 TTDVMTSPALSHSREKRAKKKGICSRCGRGNRLKEREACLVCDAQYCSNCLLKAMGSMPE 240

Query: 241 GRKCVGCIGRPIDELKRAVLGKCSRILSRVCSPLEIKQIMKAEKECPANQLRPEQLIVNE 300
           GRKCVGCIGRPIDELKRAVLGKCSRILSRVCSPLEIKQIMKAEKECPANQLRPEQLIVNE
Sbjct: 241 GRKCVGCIGRPIDELKRAVLGKCSRILSRVCSPLEIKQIMKAEKECPANQLRPEQLIVNE 300

Query: 301 RQLRPEELAEILGCAIPPQKLKPGRYWYDKDSGLWGKEGEKPDRIISSKLNVGGKLRADA 360
           RQLRPEELAEILGCAIPPQKLKPGRYWYDKDSGLWGKEGEKPDRIISSKLNVGGKLRADA
Sbjct: 301 RQLRPEELAEILGCAIPPQKLKPGRYWYDKDSGLWGKEGEKPDRIISSKLNVGGKLRADA 360

Query: 361 SKGSTKVFINGREITKVELRVLKLANVQCPRDTHFWVYDDGSYEEEGQNNIKGNIWGKAS 420
           SKGSTKVFINGREITKVELRVLKLANVQCPRDTHFWVYDDGSYEEEGQNNIKGNIWGKAS
Sbjct: 361 SKGSTKVFINGREITKVELRVLKLANVQCPRDTHFWVYDDGSYEEEGQNNIKGNIWGKAS 420

Query: 421 TRLLCSLFSLPVPPVNLHAPKDDPTTISGRSVPDYLEHGRIHKLLLFGIEGSGTSTLFKQ 480
           TRLLCSLFSLPVPPVNLHAPK+DPTTISGRSVPDYLEHGRIHKLLLFGIEGSGTSTLFKQ
Sbjct: 421 TRLLCSLFSLPVPPVNLHAPKEDPTTISGRSVPDYLEHGRIHKLLLFGIEGSGTSTLFKQ 480

Query: 481 ARFLYGNKFSPEELQNIKLMIQSNVYKYLSTLLEGRERFEEEAIIEKKTGAVIEEKLDKK 540
           ARFLYGNKF+PEELQNIKLMIQSNVYKYLSTLLEGRERFEEEAIIEKKTGAVIEEKLDKK
Sbjct: 481 ARFLYGNKFTPEELQNIKLMIQSNVYKYLSTLLEGRERFEEEAIIEKKTGAVIEEKLDKK 540

Query: 541 AGTAETGVTESKQCLYSINQRFKHFSDWLLNIMATGDLDAFFPAATREYAPIVDEMWKDS 600
           AGTAETGVTESKQCLYSINQRFKHFSDWLLNIMATGDLDAFFPAATREYAPIVDEMWKDS
Sbjct: 541 AGTAETGVTESKQCLYSINQRFKHFSDWLLNIMATGDLDAFFPAATREYAPIVDEMWKDS 600

Query: 601 ALQETYRRRDELHSLPDVTKYFLDRVIEISSNEYEPSDKDILYAEGVSQSNGLAFMEFYF 660
           ALQETYRRRDELHSLPDVTKYFLDRVIEISSNEYEPSDKDILYAEGVSQSNGLAFMEFYF
Sbjct: 601 ALQETYRRRDELHSLPDVTKYFLDRVIEISSNEYEPSDKDILYAEGVSQSNGLAFMEFYF 660

Query: 661 DDRSPVSELYGEN 674
           DDRSPVSELY  N
Sbjct: 661 DDRSPVSELYDTN 673

BLAST of Cucsa.112140 vs. NCBI nr
Match: gi|595852789|ref|XP_007210375.1| (hypothetical protein PRUPE_ppa001215mg [Prunus persica])

HSP 1 Score: 1289.2 bits (3335), Expect = 0.0e+00
Identity = 638/882 (72.34%), Postives = 735/882 (83.33%), Query Frame = 1

Query: 1   MASDTDEENAWQEAIRRMLPAGAPLPDEEHLDYSIAVEYQGPPVAHE-LPKIDPLDLESL 60
           MAS+ ++E +W++ +R+MLPAGAPLPDEEHLDYSIAVEYQGPP+ ++ LP++DP+ ++SL
Sbjct: 1   MASEAEDEKSWKDLLRKMLPAGAPLPDEEHLDYSIAVEYQGPPLPYDDLPRVDPVQIDSL 60

Query: 61  SILSPSVVPISDVSSIPIVYPIAPRVSRFNRLSNGAVPRSPVKSQR-SSSVPRVQLELHS 120
              S + + +SD+SSIP+  P+A ++SRFNR+ NGA  + P  S   SSS P+ QL+L +
Sbjct: 61  QTSSVTSLSVSDLSSIPVAVPVAQKLSRFNRVRNGAGVKEPGSSSTTSSSAPKSQLDLQN 120

Query: 121 GERSKFGNGVDEVFSSELSAQHLNSESEP-AIVEGKLANTVTFVTPRDSE-------DDE 180
           G       G D+ FSSEL  Q  N + +P     GK A  VTF TPRDSE       D+E
Sbjct: 121 GREGSEFEGADQGFSSELPVQDSNPQQKPIGTTGGKRAAVVTFNTPRDSENEDDHENDNE 180

Query: 181 EDVFSSPRSCTTDVMTSP-ALSHSRDKRAKKKGICSRCGRGNRLKEREACLVCDAQYCSN 240
            D  SSP+S  T+ + SP AL+ S  +R  K+GICSRCG+GNRLKERE CLVCDA++CSN
Sbjct: 181 ADRSSSPQSSATEPLGSPIALASSPGRRTNKRGICSRCGKGNRLKEREWCLVCDAKFCSN 240

Query: 241 CLLKTMGSMPEGRKCVGCIGRPIDELKRAVLGKCSRILSRVCSPLEIKQIMKAEKECPAN 300
           CLLK MGSMPEGRKCV CIG+PIDE KR+ LGKCSRILSRVCSPLEI+QIMKAEKECPAN
Sbjct: 241 CLLKAMGSMPEGRKCVSCIGQPIDESKRSSLGKCSRILSRVCSPLEIRQIMKAEKECPAN 300

Query: 301 QLRPEQLIVNERQLRPEELAEILGCAIPPQKLKPGRYWYDKDSGLWGKEGEKPDRIISSK 360
           QLRPEQL+VN R LR EELAEILGC +PPQKLKPGRYWYDKDSGLWGKEGEKPDRIISSK
Sbjct: 301 QLRPEQLVVNGRLLREEELAEILGCELPPQKLKPGRYWYDKDSGLWGKEGEKPDRIISSK 360

Query: 361 LNVGGKLRADASKGSTKVFINGREITKVELRVLKLANVQCPRDTHFWVYDDGSYEEEGQN 420
           LNVGGKLR DAS G+TKVF+NGREITK E RVLKLA VQCP DTHFWVYDDGSYEEEGQN
Sbjct: 361 LNVGGKLRFDASNGNTKVFMNGREITKTERRVLKLAKVQCPPDTHFWVYDDGSYEEEGQN 420

Query: 421 NIKGNIWGKASTRLLCSLFSLPVPPVNLHAPKDDPTTISGRSVPDYLEHGRIHKLLLFGI 480
           NIKGNIWGKASTR +CSLFSLPVPP N H   +DPTT S RSVP+YLE GR+ KLLLFG+
Sbjct: 421 NIKGNIWGKASTRFICSLFSLPVPPGNQHGTNEDPTTPSSRSVPEYLEQGRVQKLLLFGL 480

Query: 481 EGSGTSTLFKQARFLYGNKFSPEELQNIKLMIQSNVYKYLSTLLEGRERFEEEAIIEKK- 540
           EGSGTST+FKQA+FLYGNKF+PEELQNIKLMIQSN+YKYLS LLEGRERFEEEA+ E + 
Sbjct: 481 EGSGTSTIFKQAKFLYGNKFTPEELQNIKLMIQSNMYKYLSILLEGRERFEEEALTENRA 540

Query: 541 TGAVIEEKLDKKAGTAETGVTESKQCLYSINQRFKHFSDWLLNIMATGDLDAFFPAATRE 600
           T +  EE        + + V ES+QC+Y+INQRFKHFSDWLL+IMATGDLDAFFPAATRE
Sbjct: 541 TSSAAEE--------SPSVVDESEQCIYAINQRFKHFSDWLLDIMATGDLDAFFPAATRE 600

Query: 601 YAPIVDEMWKDSALQETYRRRDELHSLPDVTKYFLDRVIEISSNEYEPSDKDILYAEGVS 660
           YAPIVDE+WKDSA+QETY+RR+ELH LP+V KYFLDR IEISSNEYEPS+ DILYAEGV+
Sbjct: 601 YAPIVDEVWKDSAIQETYKRREELHCLPEVAKYFLDRAIEISSNEYEPSENDILYAEGVT 660

Query: 661 QSNGLAFMEFYFDDRSPVSELYGENLELPPPLTKYQLIQINSEGLVDGCKWLDMFEDVRA 720
           QSNGLAFMEF FDDRS +SELY EN E PPPLTKYQLI+INS+GL DGCKWL+MFEDVRA
Sbjct: 661 QSNGLAFMEFSFDDRSAMSELYNENYECPPPLTKYQLIRINSKGLNDGCKWLEMFEDVRA 720

Query: 721 MIFCVSLSDYDQMLSHSKGPLQNKMLASRNLFEHLVRHPCFGNTPFLLLLNKYDAFEEKI 780
           +IFCV+LSDYDQM +   G L NKMLASR+LFE LVRHPCF NTPF+LLLNKYDAFE KI
Sbjct: 721 VIFCVALSDYDQMWAQGNGLLCNKMLASRDLFESLVRHPCFRNTPFVLLLNKYDAFEAKI 780

Query: 781 NQVPLSSCEWFQDFCPVKPHNNSQTLAQQAYYYIAVKFKELYFSISGQKLFVWHTRARER 840
           NQVPLS CEW +DF PVKPH N Q+LAQQAYYY+AVKFKELY SI+G+KLFV  TRAR  
Sbjct: 781 NQVPLSVCEWLKDFSPVKPHGNIQSLAQQAYYYVAVKFKELYSSITGEKLFVAQTRARAG 840

Query: 841 ASVDEAFRYIREVLKWEEEKNDSMYDIVGDESFYSTEISSSP 871
            SVDEAF+YIRE+LKW++EKND+ + + GD+SFYSTE+SSSP
Sbjct: 841 TSVDEAFKYIREILKWDDEKNDNFFAMNGDDSFYSTEMSSSP 874