Cucsa.108530 (gene) Cucumber (Gy14) v1

Overview
NameCucsa.108530
Typegene
OrganismCucumis sativus (Cucumber (Gy14) v1)
DescriptionRetrovirus-related Pol polyprotein from transposon TNT 1-94
Locationscaffold00931 : 664833 .. 668094 (-)
Genome Viewer
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATTTCGAGACTTCTATCTCACCTTAGAAAGCAATTCAATACAAAGATTGCAATCCTACACAGTGGTAATTGTCGTGAATTTCAAAACCACACCTTTAACGAGTTTCTGACGTCTGAAGGAATTGTTCACCAAAGTCTTTGTGCTTACTGATGGAATCTGTAGGAAGAAATAGTAATTCTATATTATTGATAGAAGTAACAGAAATACAATCCTCTGCTTTCCTCTTTCTCAAGGAATAACAGAAAAGATTCTGAACTTCAAAATCCCAATAACTCCCTCTCCCGCCCTTTACAAACTTATTTATATTGCTACCCTTCCCTTCACTCACACGTGCCTCACCCTCACGTGTTATTTTTTATTTCCTCTCCTACTGTACTCATATAATATTGGAGGTCTATCATTTACACCCCTAACAGAATTGGGTTACTGAGCGAAAGAACCGTCATCTATTGAAAGTTGCTCGTTCTCTTATATTGTCTACTTCCCTTCTTTCCTATCTACGAGGGCGATGTTCTTATTGCACTTATCTCATCGACCGAATGCCTTCCTGTGTTCTACACCTCTAAACACCCCTAGACTACCTCAAGGAATCCTACCCCTCCACCCGTCTCATCTATGATGTTCCCCTTTGGGTGTTCATGTGAATAACCTATGCTCATAGCCATGATTGAAACCAAACTAAATTCTCTCCCTGGGCATAGACTTGCGTGTTTGTTGGTTATCCTATGCAGCAACAAGGCTATAAACGTTTCCATCGATCTTTGTGTAAGTACTTTGTTACCATGGATGTCACCTTTCATGAAGATTGTCCTTTCTTTCTTGTTAGCCTACTTCAGGTAGAGAGTGTGAGTGAAGAGTCTAACTATGTGGTTTCTTTGGAGTCAACCTATCACAATGGTTACTTTACCTAACTTAAATCCCCACAGTTCAATCCTACCTACAAATCAAGTTCCTTGGAGAACCTACTATAAAAGGAATCTCATAAAGGAAGTCAGTTTTTTACTGTTCAGCTGACTCCAGTCCAGGATTCTGAACCGTTATGAGATCAAGTTATGACTGACTCTATTGACTCAAATTTTAAAAACATGATTAGTGAGAATGACAGGTCCGAGACGAATTCCACTAGCTCTTGTATTGACAATAATGTGGGTGAAAATGACTGATTTGTGAATGACGGGTCCGAGATAGCTGTCCCTGAGAATATGGTTGAGAAGAGTAGTGTTGATGAAGTCATCAAAGACAGAGAGGATAATATTGATAAAAATGAGGTCATTATAGAGTTCACTAAAAACATTATGATACCAGGTCCTGTACCAAGCACTCCATCTCTAATTATGTTTCGTACTAGAACCTCTGACATTAGTTCTAGGCTTTCTTTGTTAGCCTTGACTCTACCACAATATCCAAGAATATCCACCTCGCCTTAGAGTGCCCTGAGTGGAAGATTGCTGTCATGTAAGAAATTAGAGACCTGGGATCTTTGCACACTCCCCAAGGGACACAAGACTTTGGGATGCAAATGGGTGTTTGCACTCAAGTACAGAGCAAATGATACCCTTGTCAGATATAAGGCCAGGTTGGTTGAAAAAGGGTTCAATCAGACTTATGAGGTTGACTATTCTGAGACATTTTCCTCTTTTGCAAAGTTTAATACATTCGAGTCTTGTTGTCTGTTACTTTAAATAAAGAATAACCCCTATATCAACTTGACGTTAAGAATGTGTTTTTGAATGGAGATTTAGAAGAGGAGTGTATATGAGTCCTCTAAGTTTGAAGCTCAGTTTATAATTAGGTTTGCAAGCTTTGGAAGTCTTTGTATGGGTTGAAAGAATCACCAAGTGCTTGGTTTGACAGGTTCACCACTTTTGTCAAGTCTTAGGGATACAATCAAGGGCATTTCGATCACACTTTGTTCACAAAGGTCTCCAAGACTAACAAAATTGCTGTATTGATTGTCTATATTGATTACATCATGTTATCTGTAGATGACATTGTTGAAATCATCCAACTAAAAAAGAAGATGAGGGATGAATTTGAAATTAAAGACTTGGAAATCTGAAGTATTTCCTTTGGATGGAGGTTGCTAGGTCCAGAGAGGGCATCTCTATGTCCTAGGGAAAGTACACCATTGATTTATTAGTTGAGACAATTATAATGGAATGTCGTTCTGCTGATATGAGTTCAATGCAGACTAAAAAATTCATCTCCTATGCTGTGAGTATTGTGAGTTAATTCATGCTAGCTCCTTACGAAGACCACATGGAAACGGTTAATATAATTCTAAGGTACTTAAAAGCAAGACCTAGTAAAGGATTGAGGTTCGGAAAGATTGATAGAAGGTGCATGCTAAGCTCCAAGGTACCTGCAGAATACTCAACCAACACACACACAATGCAGAATATTCAACCAGCACACCCACGACACAATAGTATTGGGAGGAATAGATGTATATACTCAACCAACACACACACAATGCAGAATATTCAACCAGCACACCCACGACACAATAGTATTGGGAGGAATAGATGTATATAATCTGTATTGATCCATAACACTACAAAAGATGTTCAACAATATTTTTTCAGCCAAGAGGGCTGTTCTCTCTCAATGAACAATGTTCATACAAAATGCACAACATTCAGAATGATGAACCCTAGCACAAAACACTTAGATATATACTCCTCTCATTCTGCTGCCACGTGGTATTAGGATCACCACTGACCAACCTAACAACCACATTATCTAGGCTGAAATTCCCTTCTTACCCCTCCTTTTATACATTTTGGTGATCAGTGGCCTAGTAGTGCATTGAAGCCTATACTGACGTTGATTGGGCATGGTCTATTGTTGATATAAAATCCACCTTGGGATACTGCACCTTTGTATGGGGCAATCTTGTTACTTGGAGAAGCAAGAAGCAAAGAGTTGTGGCTAGAAGTAGTGCTGAAGCTGAATAGAAGGCTATGAGTTTGAGAATGTGTTGTCTAATCTTTGTCAGAACTATGAGGTGTCTAAGAAACTATTATGTGATAACAAGACAACTATCAACATTGCCACTAATCCAGTCAAAGATGACAAAACCAAACACGTGGAGATTGACAAACACTTTTTCAAAGAGAGACTGGACAGTGGTAGCATCTGTATCCCTTACATACCCTCGAGCCATCAAATTGCTGATATACTCACGAAGAGGCTCCTGAGACACAACTTTGATTCATGTGTTAGCAAGTTAGCATTTTCTGACATATATGTCCCAACATAG

mRNA sequence

atttcgagacttctatctcaccttagaaagcaattcaatacaaagattgcaatcctacacagtggtaattgtcgtgaatttcaaaaccacacctttaacgagtttctgacgtctgaaggaattaattgggttactgagcgaaagaaccgtcatctattgaaagttgctcgttctcttatattgtctacttcccttctttcctatctacgagggcgatgttcttattgcacttatctcatcgaccgaatgccttcctgtacttgcgtgtttgttggttatcctatgcagcaacaaggctataaacgtttccatcgatctttgtgtaagtactttgttaccatggatgtcacctttcatgaagattgtcctttctttcttgttagcctacttcaggtagagagtacctgggatctttgcacactccccaagggacacaagactttgggatgcaaatgggtgtttgcactcaagtacagagcaaatgatacccttgtcagatataaggccaggttggttgaaaaagggttcaatcagacttatgaggttgactattctgagacattttcctcttttgcaaagtttaatacattcgagtcttgttctccttacgaagaccacatggaaacggttaatataattctaaggtacttaaaagcaagacctagtaaaggattgaggttcggaaagattgatagaaggTGCatgctaagctccaagTGcattgaagcctatactgacgttgattgggcatggtctattgttgatataaaatccaccttgggatactgcacctttgtatggggcaatcttgttacttggagaagcaagaagcaaagagttaactatgaggtgtctaagaaactattatgtgataacaagacaactatcaacattgccactaatccagtcaaagatgacaaaaccaaacacgtggagattgacaaacactttttcaaagagagactggacagtggtagcatctgtatcccttacataccctcgagccatcaaattgctgatatactcacgaagaggctcctgagacacaactttgattcatgtgttagcaagttagcattttctgacatatatgtcccaacatag

Coding sequence (CDS)

ATTTCGAGACTTCTATCTCACCTTAGAAAGCAATTCAATACAAAGATTGCAATCCTACACAGTGGTAATTGTCGTGAATTTCAAAACCACACCTTTAACGAGTTTCTGACGTCTGAAGGAATTAATTGGGTTACTGAGCGAAAGAACCGTCATCTATTGAAAGTTGCTCGTTCTCTTATATTGTCTACTTCCCTTCTTTCCTATCTACGAGGGCGATGTTCTTATTGCACTTATCTCATCGACCGAATGCCTTCCTGTACTTGCGTGTTTGTTGGTTATCCTATGCAGCAACAAGGCTATAAACGTTTCCATCGATCTTTGTGTAAGTACTTTGTTACCATGGATGTCACCTTTCATGAAGATTGTCCTTTCTTTCTTGTTAGCCTACTTCAGGTAGAGAGTACCTGGGATCTTTGCACACTCCCCAAGGGACACAAGACTTTGGGATGCAAATGGGTGTTTGCACTCAAGTACAGAGCAAATGATACCCTTGTCAGATATAAGGCCAGGTTGGTTGAAAAAGGGTTCAATCAGACTTATGAGGTTGACTATTCTGAGACATTTTCCTCTTTTGCAAAGTTTAATACATTCGAGTCTTGTTCTCCTTACGAAGACCACATGGAAACGGTTAATATAATTCTAAGGTACTTAAAAGCAAGACCTAGTAAAGGATTGAGGTTCGGAAAGATTGATAGAAGGTGCATGCTAAGCTCCAAGTGCATTGAAGCCTATACTGACGTTGATTGGGCATGGTCTATTGTTGATATAAAATCCACCTTGGGATACTGCACCTTTGTATGGGGCAATCTTGTTACTTGGAGAAGCAAGAAGCAAAGAGTTAACTATGAGGTGTCTAAGAAACTATTATGTGATAACAAGACAACTATCAACATTGCCACTAATCCAGTCAAAGATGACAAAACCAAACACGTGGAGATTGACAAACACTTTTTCAAAGAGAGACTGGACAGTGGTAGCATCTGTATCCCTTACATACCCTCGAGCCATCAAATTGCTGATATACTCACGAAGAGGCTCCTGAGACACAACTTTGATTCATGTGTTAGCAAGTTAGCATTTTCTGACATATATGTCCCAACATAG

Protein sequence

ISRLLSHLRKQFNTKIAILHSGNCREFQNHTFNEFLTSEGINWVTERKNRHLLKVARSLILSTSLLSYLRGRCSYCTYLIDRMPSCTCVFVGYPMQQQGYKRFHRSLCKYFVTMDVTFHEDCPFFLVSLLQVESTWDLCTLPKGHKTLGCKWVFALKYRANDTLVRYKARLVEKGFNQTYEVDYSETFSSFAKFNTFESCSPYEDHMETVNIILRYLKARPSKGLRFGKIDRRCMLSSKCIEAYTDVDWAWSIVDIKSTLGYCTFVWGNLVTWRSKKQRVNYEVSKKLLCDNKTTINIATNPVKDDKTKHVEIDKHFFKERLDSGSICIPYIPSSHQIADILTKRLLRHNFDSCVSKLAFSDIYVPT*
Homology
[Close]
BLAST of Cucsa.108530 vs. Swiss-Prot
Match: COPIA_DROME (Copia protein OS=Drosophila melanogaster GN=GIP PE=1 SV=3)

HSP 1 Score: 78.6 bits (192), Expect = 1.6e-13
Identity = 57/179 (31.84%), Postives = 80/179 (44.69%), Query Frame = 1 

Query: 213  ILRYLKARPSKGLRFGKIDRRCMLSSKCIEAYTDVDWAWSIVDIKSTLGYCTFVWG-NLV 272
            +LRYLK      L F K        +K I  Y D DWA S +D KST GY   ++  NL+
Sbjct: 1224 VLRYLKGTIDMKLIFKK---NLAFENKII-GYVDSDWAGSEIDRKSTTGYLFKMFDFNLI 1283

Query: 273  TWRSKKQ--------------------------------RVNYEVSKKLLCDNKTTINIA 332
             W +K+Q                                 +  E   K+  DN+  I+IA
Sbjct: 1284 CWNTKRQNSVAASSTEAEYMALFEAVREALWLKFLLTSINIKLENPIKIYEDNQGCISIA 1343

Query: 333  TNPVKDDKTKHVEIDKHFFKERLDSGSICIPYIPSSHQIADILTKRLLRHNFDSCVSKL 359
             NP    + KH++I  HF +E++ +  IC+ YIP+ +Q+ADI TK L    F     KL
Sbjct: 1344 NNPSCHKRAKHIDIKYHFAREQVQNNVICLEYIPTENQLADIFTKPLPAARFVELRDKL 1398


HSP 2 Score: 66.2 bits (160), Expect = 8.3e-10
Identity = 26/67 (38.81%), Postives = 43/67 (64.18%), Query Frame = 1 

Query: 131 QVESTWDLCTLPKGHKTLGCKWVFALKYRANDTLVRYKARLVEKGFNQTYEVDYSETFSS 190
           ++ +TW +   P+    +  +WVF++KY      +RYKARLV +GF Q Y++DY ETF+ 
Sbjct: 918 KINNTWTITKRPENKNIVDSRWVFSVKYNELGNPIRYKARLVARGFTQKYQIDYEETFAP 977

Query: 191 FAKFNTF 198
            A+ ++F
Sbjct: 978 VARISSF 984

[Close]
BLAST of Cucsa.108530 vs. Swiss-Prot
Match: POLX_TOBAC (Retrovirus-related Pol polyprotein from transposon TNT 1-94 OS=Nicotiana tabacum PE=2 SV=1)

HSP 1 Score: 67.4 bits (163), Expect = 3.7e-10
Identity = 34/70 (48.57%), Postives = 41/70 (58.57%), Query Frame = 1 

Query: 130 LQVESTWDLCTLPKGHKTLGCKWVFALKYRANDTLVRYKARLVEKGFNQTYEVDYSETFS 189
           LQ   T+ L  LPKG + L CKWVF LK   +  LVRYKARLV KGF Q   +D+ E FS
Sbjct: 837 LQKNGTYKLVELPKGKRPLKCKWVFKLKKDGDCKLVRYKARLVVKGFEQKKGIDFDEIFS 896

Query: 190 SFAKFNTFES 200
              K  +  +
Sbjct: 897 PVVKMTSIRT 906

[Close]
BLAST of Cucsa.108530 vs. Swiss-Prot
Match: M820_ARATH (Uncharacterized mitochondrial protein AtMg00820 OS=Arabidopsis thaliana GN=AtMg00820 PE=4 SV=1)

HSP 1 Score: 57.4 bits (137), Expect = 3.9e-07
Identity = 34/96 (35.42%), Postives = 44/96 (45.83%), Query Frame = 1 

Query: 130 LQVESTWDLCTLPKGHKTLGCKWVFALKYRANDTLVRYKARLVEKGFNQTYEVDYSETFS 189
           L    TW L   P     LGCKWVF  K  ++ TL R KARLV KGF+Q   + + ET+S
Sbjct: 51  LSRNKTWILVPPPVNQNILGCKWVFKTKLHSDGTLDRLKARLVAKGFHQEEGIYFVETYS 110

Query: 190 SFAKFNTFESCSPYEDHMETVNIILRYLKARPSKGL 226
              +  T  +       +E    I    K   S G+
Sbjct: 111 PVVRTATIRTILNVAQQLEVGQSINWMFKMHFSMGI 146

[Close]
BLAST of Cucsa.108530 vs. TrEMBL
Match: A5AYJ3_VITVI (Putative uncharacterized protein OS=Vitis vinifera GN=VITISV_041073 PE=4 SV=1)

HSP 1 Score: 190.3 bits (482), Expect = 4.3e-45
Identity = 99/199 (49.75%), Postives = 123/199 (61.81%), Query Frame = 1 

Query: 201  SPYEDHMETVNIILRYLKARPSKGLRFGKIDRRCMLSSKCIEAYTDVDWAWSIVDIKSTL 260
            SPYE H+E V  ILRYLK+ P KGL F K ++      K IEAYTD DWA S+ D +ST 
Sbjct: 1313 SPYEVHLEAVYRILRYLKSTPGKGLFFKKSEQ------KTIEAYTDADWAGSVTDRRSTS 1372

Query: 261  GYCTFVWGNLVTWRSKKQRVN--------------------------------YEVSKKL 320
            GYCT++WGNLVTWRSKKQ V                                  E+  KL
Sbjct: 1373 GYCTYIWGNLVTWRSKKQSVXARSSAEAEYRAMAHGVCEILWLKKILEELKRPLEMPMKL 1432

Query: 321  LCDNKTTINIATNPVKDDKTKHVEIDKHFFKERLDSGSICIPYIPSSHQIADILTKRLLR 368
             CDNK  I+IA NPV+ D+TKHVEID+HF KE+L++  IC+P++P++ QIADILTK L R
Sbjct: 1433 YCDNKAAISIAHNPVQHDRTKHVEIDRHFIKEKLEASIICMPFVPTTQQIADILTKGLFR 1492

[Close]
BLAST of Cucsa.108530 vs. TrEMBL
Match: A5AYJ3_VITVI (Putative uncharacterized protein OS=Vitis vinifera GN=VITISV_041073 PE=4 SV=1)

HSP 1 Score: 90.1 bits (222), Expect = 6.0e-15
Identity = 40/67 (59.70%), Postives = 49/67 (73.13%), Query Frame = 1 

Query: 130  LQVESTWDLCTLPKGHKTLGCKWVFALKYRANDTLVRYKARLVEKGFNQTYEVDYSETFS 189
            L+   TW++  LPKG  T+GCKWVF +KY +N +L RYKARLV KGF QTY +DY ETF+
Sbjct: 1018 LEKNHTWEVMGLPKGKTTVGCKWVFTVKYNSNGSLERYKARLVAKGFTQTYGIDYLETFA 1077

Query: 190  SFAKFNT 197
              AK NT
Sbjct: 1078 PVAKLNT 1084


HSP 2 Score: 60.5 bits (145), Expect = 5.1e-06
Identity = 35/88 (39.77%), Postives = 48/88 (54.55%), Query Frame = 1 

Query: 11  QFNTKIAILHSGNCREFQNHTFNEFLTSEGI------------NWVTERKNRHLLKVARS 70
           QF TKI +  S N +E+ N    +F   +GI            N + ERKN+HLL+VAR+
Sbjct: 609 QFQTKIQVFRSDNGKEYINKALGKFFLEKGIVHQSSCNDTPQQNGIAERKNKHLLEVARA 668

Query: 71  LILSTSLLSYLRGRCSY-CTYLIDRMPS 86
           L  +T +  YL G      TYLI+RMP+
Sbjct: 669 LCFTTKVPKYLWGEAILTATYLINRMPT 696


HSP 3 Score: 52.4 bits (124), Expect = 1.4e-03
Identity = 33/60 (55.00%), Postives = 36/60 (60.00%), Query Frame = 1 

Query: 88  CVFVGYPMQQQGYKRFHRSLCKYFVTMDVTFHEDCPFFLVSLLQVESTWDLCTLPKGHKT 147
           CVFVGY   Q+GYK F     K FVTMDVTF E  PFF  + LQ EST +   L K  KT
Sbjct: 749 CVFVGYAPTQKGYKCFDPISKKLFVTMDVTFFESKPFF-ATHLQGESTSEDSDLFKIEKT 807


HSP 4 Score: 181.8 bits (460), Expect = 1.5e-42
Identity = 96/199 (48.24%), Postives = 120/199 (60.30%), Query Frame = 1 

Query: 201 SPYEDHMETVNIILRYLKARPSKGLRFGKIDRRCMLSSKCIEAYTDVDWAWSIVDIKSTL 260
           SPYE H+E V  ILRYLK+ P KGL F K  +      K IEAYTD DWA  + D +S+ 
Sbjct: 716 SPYEVHLEAVYRILRYLKSTPGKGLFFKKSKQ------KTIEAYTDADWAGLVTDRRSSS 775

Query: 261 GYCTFVWGNLVTWRSKKQRV--------------------------------NYEVSKKL 320
           GYCT++WGNLVTWRSKKQ V                                  E+  KL
Sbjct: 776 GYCTYIWGNLVTWRSKKQSVVARSSAEAEYRAMAHGVCEILWLKKILEELKRPLEMPMKL 835

Query: 321 LCDNKTTINIATNPVKDDKTKHVEIDKHFFKERLDSGSICIPYIPSSHQIADILTKRLLR 368
            CDNK  I+IA NPV+ D+TKHVEID+HF KE+L++  IC+ ++P++ QIADILTK L R
Sbjct: 836 YCDNKAAISIAHNPVQHDRTKHVEIDRHFIKEKLEASIICMSFVPTTQQIADILTKGLFR 895

[Close]
BLAST of Cucsa.108530 vs. TrEMBL
Match: A5CAQ8_VITVI (Putative uncharacterized protein OS=Vitis vinifera GN=VITISV_017186 PE=4 SV=1)

HSP 1 Score: 88.6 bits (218), Expect = 1.7e-14
Identity = 39/67 (58.21%), Postives = 49/67 (73.13%), Query Frame = 1 

Query: 130 LQVESTWDLCTLPKGHKTLGCKWVFALKYRANDTLVRYKARLVEKGFNQTYEVDYSETFS 189
           L+   TW++  LPKG  T+GCKWVF +KY +N +L RYKARLV KGF QTY +DY +TF+
Sbjct: 456 LEKNHTWEVMGLPKGKNTVGCKWVFIVKYNSNGSLERYKARLVAKGFTQTYGIDYLKTFA 515

Query: 190 SFAKFNT 197
             AK NT
Sbjct: 516 PVAKLNT 522


HSP 2 Score: 181.8 bits (460), Expect = 1.5e-42
Identity = 95/199 (47.74%), Postives = 121/199 (60.80%), Query Frame = 1 

Query: 201 SPYEDHMETVNIILRYLKARPSKGLRFGKIDRRCMLSSKCIEAYTDVDWAWSIVDIKSTL 260
           SPYE H E V  ILRYLK+ P KGL F K ++      K IEAYT+ DWA S++D KST 
Sbjct: 299 SPYEVHFEAVYRILRYLKSTPGKGLFFKKSEQ------KTIEAYTNADWAGSVIDRKSTS 358

Query: 261 GYCTFVWGNLVTWRSKKQ--------------------------------RVNYEVSKKL 320
           GYCT++WGNLVTWRSKKQ                                ++  E+  KL
Sbjct: 359 GYCTYIWGNLVTWRSKKQSVVARSSAEAEYRAMAHGVCEILWLKKILEELKIPLEMPMKL 418

Query: 321 LCDNKTTINIATNPVKDDKTKHVEIDKHFFKERLDSGSICIPYIPSSHQIADILTKRLLR 368
            CDNK  I+IA NPV+ D+TKHVEID+HF K++L++  IC+ +I ++ QI DILTK L R
Sbjct: 419 YCDNKAAISIAHNPVQHDRTKHVEIDRHFIKDKLEASIICMSFISTTQQIVDILTKGLFR 478

[Close]
BLAST of Cucsa.108530 vs. TrEMBL
Match: A5BFS0_VITVI (Putative uncharacterized protein OS=Vitis vinifera GN=VITISV_021511 PE=4 SV=1)

HSP 1 Score: 70.9 bits (172), Expect = 3.8e-09
Identity = 35/67 (52.24%), Postives = 43/67 (64.18%), Query Frame = 1 

Query: 130 LQVESTWDLCTLPKGHKTLGCKWVFALKYRANDTLVRYKARLVEKGFNQTYEVDYSETFS 189
           L+   TW++  LPKG  T+G KWV  +KY +N +L RYKARLV KG  QTY     ETF+
Sbjct: 4   LEKNHTWEVMGLPKGKTTVGXKWVXTVKYNSNGSLERYKARLVAKGGTQTYGGXDLETFA 63

Query: 190 SFAKFNT 197
             AK NT
Sbjct: 64  LVAKLNT 70


HSP 2 Score: 176.0 bits (445), Expect = 8.3e-41
Identity = 94/199 (47.24%), Postives = 117/199 (58.79%), Query Frame = 1 

Query: 201 SPYEDHMETVNIILRYLKARPSKGLRFGKIDRRCMLSSKCIEAYTDVDWAWSIVDIKSTL 260
           SP+E+H+E V   LRYLK+ P KGL        C  + + IEAYTDVDWA SI D K T 
Sbjct: 371 SPHEEHLEAVYRTLRYLKSSPGKGLF-------CKKNERSIEAYTDVDWARSITDRKPTS 430

Query: 261 GYCTFVWGNLVTWRSKKQRVNYEVS--------------------------------KKL 320
            YCTFVW NL+TW  KKQ V  + S                                 KL
Sbjct: 431 SYCTFVWENLLTWGKKKQSVVAQSSAEAEYRSMAYAVCEMMWLKRALEEIRRPATMPTKL 490

Query: 321 LCDNKTTINIATNPVKDDKTKHVEIDKHFFKERLDSGSICIPYIPSSHQIADILTKRLLR 368
            CDNK  I+IA NPV+ D+TKHVEID+HF KE++++G+IC P++P+  QIADILTK L R
Sbjct: 491 YCDNKAAISIAHNPVQYDRTKHVEIDRHFIKEKIEAGAICTPFVPTLQQIADILTKGLFR 550

[Close]
BLAST of Cucsa.108530 vs. TrEMBL
Match: A5AMR4_VITVI (Putative uncharacterized protein OS=Vitis vinifera GN=VITISV_006313 PE=4 SV=1)

HSP 1 Score: 172.6 bits (436), Expect = 9.2e-40
Identity = 90/189 (47.62%), Postives = 117/189 (61.90%), Query Frame = 1 

Query: 201  SPYEDHMETVNIILRYLKARPSKGLRFGKIDRRCMLSSKCIEAYTDVDWAWSIVDIKSTL 260
            S YE H+E V  ILRYLK+ P KGL F K ++      K IEAYTD DWA S+ D +ST 
Sbjct: 1064 SXYEVHLEAVYRILRYLKSTPGKGLFFKKSEQ------KTIEAYTDADWAGSVTDRRSTS 1123

Query: 261  GYCTFVWGNL-------------------VTWRSK---KQRVNYEVSKKLLCDNKTTINI 320
            GYCT++WGNL                   + W  K   + +   E+  KL CDNK  I+I
Sbjct: 1124 GYCTYIWGNLLARSSAEAEYRAMAHGVCEILWLKKILEELKXPLEMPMKLYCDNKAAISI 1183

Query: 321  ATNPVKDDKTKHVEIDKHFFKERLDSGSICIPYIPSSHQIADILTKRLLRHNFDSCVSKL 368
            A NP + D+TKHVEID+HF KE+L++  IC+P++P++ QIADILTK L R +F+  +SKL
Sbjct: 1184 AHNPXQHDRTKHVEIDRHFIKEKLEASIICMPFVPTTQQIADILTKGLFRSSFEFLISKL 1243

[Close]
BLAST of Cucsa.108530 vs. TAIR10
Match: AT4G23160.1 (AT4G23160.1 cysteine-rich RLK (RECEPTOR-like protein kinase) 8)

HSP 1 Score: 78.2 bits (191), Expect = 1.2e-14
Identity = 31/70 (44.29%), Postives = 46/70 (65.71%), Query Frame = 1 

Query: 127 VSLLQVESTWDLCTLPKGHKTLGCKWVFALKYRANDTLVRYKARLVEKGFNQTYEVDYSE 186
           +  ++   TW++CTLP   K +GCKWV+ +KY ++ T+ RYKARLV KG+ Q   +D+ E
Sbjct: 106 IGAMETTHTWEICTLPPNKKPIGCKWVYKIKYNSDGTIERYKARLVAKGYTQQEGIDFIE 165

Query: 187 TFSSFAKFNT 197
           TFS   K  +
Sbjct: 166 TFSPVCKLTS 175


HSP 2 Score: 61.6 bits (148), Expect = 1.2e-09
Identity = 46/176 (26.14%), Postives = 76/176 (43.18%), Query Frame = 1 

Query: 178 QTYEVDYSETFSSFAKFNTFESCSPYEDHMETVNIILRYLKARPSKGLRFGKIDRRCMLS 237
           Q   +D S   +  ++F+     +P   H + V  IL Y+K    +GL +         +
Sbjct: 389 QITRLDISFAVNKLSQFSE----APRLAHQQAVMKILHYIKGTVGQGLFYSS------QA 448

Query: 238 SKCIEAYTDVDWAWSIVDIKSTLGYCTFVWGNLVTWRSKKQRVNYEVSKK---------- 297
              ++ ++D  +       +ST GYC F+  +L++W+SKKQ+V  + S +          
Sbjct: 449 EMQLQVFSDASFQSCKDTRRSTNGYCMFLGTSLISWKSKKQQVVSKSSAEAEYRALSFAT 508

Query: 298 ----------------------LLCDNKTTINIATNPVKDDKTKHVEIDKHFFKER 322
                                 L CDN   I+IATN V  ++TKH+E D H  +ER
Sbjct: 509 DEMMWLAQFFRELQLPLSKPTLLFCDNTAAIHIATNAVFHERTKHIESDCHSVRER 554

[Close]
BLAST of Cucsa.108530 vs. TAIR10
Match: ATMG00820.1 (ATMG00820.1 Reverse transcriptase (RNA-dependent DNA polymerase))

HSP 1 Score: 57.4 bits (137), Expect = 2.2e-08
Identity = 34/96 (35.42%), Postives = 44/96 (45.83%), Query Frame = 1 

Query: 130 LQVESTWDLCTLPKGHKTLGCKWVFALKYRANDTLVRYKARLVEKGFNQTYEVDYSETFS 189
           L    TW L   P     LGCKWVF  K  ++ TL R KARLV KGF+Q   + + ET+S
Sbjct: 51  LSRNKTWILVPPPVNQNILGCKWVFKTKLHSDGTLDRLKARLVAKGFHQEEGIYFVETYS 110

Query: 190 SFAKFNTFESCSPYEDHMETVNIILRYLKARPSKGL 226
              +  T  +       +E    I    K   S G+
Sbjct: 111 PVVRTATIRTILNVAQQLEVGQSINWMFKMHFSMGI 146

[Close]
BLAST of Cucsa.108530 vs. NCBI nr
Match: gi|147819777|emb|CAN76196.1| (hypothetical protein VITISV_041073 [Vitis vinifera])

HSP 1 Score: 190.3 bits (482), Expect = 6.1e-45
Identity = 99/199 (49.75%), Postives = 123/199 (61.81%), Query Frame = 1 

Query: 201  SPYEDHMETVNIILRYLKARPSKGLRFGKIDRRCMLSSKCIEAYTDVDWAWSIVDIKSTL 260
            SPYE H+E V  ILRYLK+ P KGL F K ++      K IEAYTD DWA S+ D +ST 
Sbjct: 1313 SPYEVHLEAVYRILRYLKSTPGKGLFFKKSEQ------KTIEAYTDADWAGSVTDRRSTS 1372

Query: 261  GYCTFVWGNLVTWRSKKQRVN--------------------------------YEVSKKL 320
            GYCT++WGNLVTWRSKKQ V                                  E+  KL
Sbjct: 1373 GYCTYIWGNLVTWRSKKQSVXARSSAEAEYRAMAHGVCEILWLKKILEELKRPLEMPMKL 1432

Query: 321  LCDNKTTINIATNPVKDDKTKHVEIDKHFFKERLDSGSICIPYIPSSHQIADILTKRLLR 368
             CDNK  I+IA NPV+ D+TKHVEID+HF KE+L++  IC+P++P++ QIADILTK L R
Sbjct: 1433 YCDNKAAISIAHNPVQHDRTKHVEIDRHFIKEKLEASIICMPFVPTTQQIADILTKGLFR 1492

[Close]
BLAST of Cucsa.108530 vs. NCBI nr
Match: gi|147819777|emb|CAN76196.1| (hypothetical protein VITISV_041073 [Vitis vinifera])

HSP 1 Score: 90.1 bits (222), Expect = 8.6e-15
Identity = 40/67 (59.70%), Postives = 49/67 (73.13%), Query Frame = 1 

Query: 130  LQVESTWDLCTLPKGHKTLGCKWVFALKYRANDTLVRYKARLVEKGFNQTYEVDYSETFS 189
            L+   TW++  LPKG  T+GCKWVF +KY +N +L RYKARLV KGF QTY +DY ETF+
Sbjct: 1018 LEKNHTWEVMGLPKGKTTVGCKWVFTVKYNSNGSLERYKARLVAKGFTQTYGIDYLETFA 1077

Query: 190  SFAKFNT 197
              AK NT
Sbjct: 1078 PVAKLNT 1084


HSP 2 Score: 60.5 bits (145), Expect = 7.3e-06
Identity = 35/88 (39.77%), Postives = 48/88 (54.55%), Query Frame = 1 

Query: 11  QFNTKIAILHSGNCREFQNHTFNEFLTSEGI------------NWVTERKNRHLLKVARS 70
           QF TKI +  S N +E+ N    +F   +GI            N + ERKN+HLL+VAR+
Sbjct: 609 QFQTKIQVFRSDNGKEYINKALGKFFLEKGIVHQSSCNDTPQQNGIAERKNKHLLEVARA 668

Query: 71  LILSTSLLSYLRGRCSY-CTYLIDRMPS 86
           L  +T +  YL G      TYLI+RMP+
Sbjct: 669 LCFTTKVPKYLWGEAILTATYLINRMPT 696


HSP 3 Score: 52.4 bits (124), Expect = 2.0e-03
Identity = 33/60 (55.00%), Postives = 36/60 (60.00%), Query Frame = 1 

Query: 88  CVFVGYPMQQQGYKRFHRSLCKYFVTMDVTFHEDCPFFLVSLLQVESTWDLCTLPKGHKT 147
           CVFVGY   Q+GYK F     K FVTMDVTF E  PFF  + LQ EST +   L K  KT
Sbjct: 749 CVFVGYAPTQKGYKCFDPISKKLFVTMDVTFFESKPFF-ATHLQGESTSEDSDLFKIEKT 807


HSP 4 Score: 181.8 bits (460), Expect = 2.2e-42
Identity = 96/199 (48.24%), Postives = 120/199 (60.30%), Query Frame = 1 

Query: 201 SPYEDHMETVNIILRYLKARPSKGLRFGKIDRRCMLSSKCIEAYTDVDWAWSIVDIKSTL 260
           SPYE H+E V  ILRYLK+ P KGL F K  +      K IEAYTD DWA  + D +S+ 
Sbjct: 716 SPYEVHLEAVYRILRYLKSTPGKGLFFKKSKQ------KTIEAYTDADWAGLVTDRRSSS 775

Query: 261 GYCTFVWGNLVTWRSKKQRV--------------------------------NYEVSKKL 320
           GYCT++WGNLVTWRSKKQ V                                  E+  KL
Sbjct: 776 GYCTYIWGNLVTWRSKKQSVVARSSAEAEYRAMAHGVCEILWLKKILEELKRPLEMPMKL 835

Query: 321 LCDNKTTINIATNPVKDDKTKHVEIDKHFFKERLDSGSICIPYIPSSHQIADILTKRLLR 368
            CDNK  I+IA NPV+ D+TKHVEID+HF KE+L++  IC+ ++P++ QIADILTK L R
Sbjct: 836 YCDNKAAISIAHNPVQHDRTKHVEIDRHFIKEKLEASIICMSFVPTTQQIADILTKGLFR 895

[Close]
BLAST of Cucsa.108530 vs. NCBI nr
Match: gi|147843633|emb|CAN79872.1| (hypothetical protein VITISV_017186 [Vitis vinifera])

HSP 1 Score: 88.6 bits (218), Expect = 2.5e-14
Identity = 39/67 (58.21%), Postives = 49/67 (73.13%), Query Frame = 1 

Query: 130 LQVESTWDLCTLPKGHKTLGCKWVFALKYRANDTLVRYKARLVEKGFNQTYEVDYSETFS 189
           L+   TW++  LPKG  T+GCKWVF +KY +N +L RYKARLV KGF QTY +DY +TF+
Sbjct: 456 LEKNHTWEVMGLPKGKNTVGCKWVFIVKYNSNGSLERYKARLVAKGFTQTYGIDYLKTFA 515

Query: 190 SFAKFNT 197
             AK NT
Sbjct: 516 PVAKLNT 522


HSP 2 Score: 181.8 bits (460), Expect = 2.2e-42
Identity = 91/199 (45.73%), Postives = 121/199 (60.80%), Query Frame = 1 

Query: 201 SPYEDHMETVNIILRYLKARPSKGLRFGKIDRRCMLSSKCIEAYTDVDWAWSIVDIKSTL 260
           SPY +H+E V+ ILRYLK+ P +GL F K D+R       +E +TD +WA  + D +ST 
Sbjct: 195 SPYAEHLEAVHKILRYLKSTPGRGLLFKKSDQRS------VEVFTDANWAGLVSDRRSTS 254

Query: 261 GYCTFVWGNLVTWRSKKQRV--------------------------------NYEVSKKL 320
           GYCTF+WGNL+TWRSKKQ V                                + E   +L
Sbjct: 255 GYCTFLWGNLITWRSKKQNVVARSSTEAEFRAMANGVCEVLWIKRVLGELKLDIEAPMRL 314

Query: 321 LCDNKTTINIATNPVKDDKTKHVEIDKHFFKERLDSGSICIPYIPSSHQIADILTKRLLR 368
           LCDNK+ I+IA NPV+ D+TKH+EID+HF KE+L++G IC+P+IPS  Q AD+LTK   R
Sbjct: 315 LCDNKSAISIANNPVQHDRTKHIEIDRHFIKEKLENGVICMPFIPSEQQTADVLTKGHFR 374

[Close]
BLAST of Cucsa.108530 vs. NCBI nr
Match: gi|147790213|emb|CAN65603.1| (hypothetical protein VITISV_021511 [Vitis vinifera])

HSP 1 Score: 181.8 bits (460), Expect = 2.2e-42
Identity = 95/199 (47.74%), Postives = 121/199 (60.80%), Query Frame = 1 

Query: 201 SPYEDHMETVNIILRYLKARPSKGLRFGKIDRRCMLSSKCIEAYTDVDWAWSIVDIKSTL 260
           SPYE H E V  ILRYLK+ P KGL F K ++      K IEAYT+ DWA S++D KST 
Sbjct: 299 SPYEVHFEAVYRILRYLKSTPGKGLFFKKSEQ------KTIEAYTNADWAGSVIDRKSTS 358

Query: 261 GYCTFVWGNLVTWRSKKQ--------------------------------RVNYEVSKKL 320
           GYCT++WGNLVTWRSKKQ                                ++  E+  KL
Sbjct: 359 GYCTYIWGNLVTWRSKKQSVVARSSAEAEYRAMAHGVCEILWLKKILEELKIPLEMPMKL 418

Query: 321 LCDNKTTINIATNPVKDDKTKHVEIDKHFFKERLDSGSICIPYIPSSHQIADILTKRLLR 368
            CDNK  I+IA NPV+ D+TKHVEID+HF K++L++  IC+ +I ++ QI DILTK L R
Sbjct: 419 YCDNKAAISIAHNPVQHDRTKHVEIDRHFIKDKLEASIICMSFISTTQQIVDILTKGLFR 478

[Close]
BLAST of Cucsa.108530 vs. NCBI nr
Match: gi|147790213|emb|CAN65603.1| (hypothetical protein VITISV_021511 [Vitis vinifera])

HSP 1 Score: 70.9 bits (172), Expect = 5.4e-09
Identity = 35/67 (52.24%), Postives = 43/67 (64.18%), Query Frame = 1 

Query: 130 LQVESTWDLCTLPKGHKTLGCKWVFALKYRANDTLVRYKARLVEKGFNQTYEVDYSETFS 189
           L+   TW++  LPKG  T+G KWV  +KY +N +L RYKARLV KG  QTY     ETF+
Sbjct: 4   LEKNHTWEVMGLPKGKTTVGXKWVXTVKYNSNGSLERYKARLVAKGGTQTYGGXDLETFA 63

Query: 190 SFAKFNT 197
             AK NT
Sbjct: 64  LVAKLNT 70


HSP 2 Score: 179.1 bits (453), Expect = 1.4e-41
Identity = 97/196 (49.49%), Postives = 115/196 (58.67%), Query Frame = 1 

Query: 204  EDHMETVNIILRYLKARPSKGLRFGKIDRRCMLSSKCIEAYTDVDWAWSIVDIKSTLGYC 263
            E+H+E V  ILRYLK+ P KGL F K + R       IE YTD DWA S++D KST GYC
Sbjct: 1079 EEHLEAVYRILRYLKSTPGKGLLFRKTENRG------IEVYTDADWAGSVIDRKSTTGYC 1138

Query: 264  TFVWGNLVTWRSKKQRV--------------------------------NYEVSKKLLCD 323
            TFVWGNLVTWRSKKQ V                                   +  +L CD
Sbjct: 1139 TFVWGNLVTWRSKKQNVVARSTAEAEFRAMAHGICEVLWLKQVLEDLRRPIVLPMRLYCD 1198

Query: 324  NKTTINIATNPVKDDKTKHVEIDKHFFKERLDSGSICIPYIPSSHQIADILTKRLLRHNF 368
            NK TI+IA NPV  D+TKHVEID+HF KE L++  IC+ ++P+  Q ADILTK LL+ NF
Sbjct: 1199 NKATISIAHNPVXHDRTKHVEIDRHFIKENLENKIICLLFVPTKQQTADILTKGLLKTNF 1258

The following BLAST results are available for this feature:
BLAST of Cucsa.108530 vs. Swiss-Prot
Match NameE-valueIdentityDescription
COPIA_DROME1.6e-1331.84Copia protein OS=Drosophila melanogaster GN=GIP PE=1 SV=3[more]
POLX_TOBAC3.7e-1048.57Retrovirus-related Pol polyprotein from transposon TNT 1-94 OS=Nicotiana tabacum... [more]
M820_ARATH3.9e-0735.42Uncharacterized mitochondrial protein AtMg00820 OS=Arabidopsis thaliana GN=AtMg0... [more]
BLAST of Cucsa.108530 vs. TrEMBL
Match NameE-valueIdentityDescription
A5AYJ3_VITVI4.3e-4549.75Putative uncharacterized protein OS=Vitis vinifera GN=VITISV_041073 PE=4 SV=1[more]
A5AYJ3_VITVI6.0e-1559.70Putative uncharacterized protein OS=Vitis vinifera GN=VITISV_041073 PE=4 SV=1[more]
A5CAQ8_VITVI1.7e-1458.21Putative uncharacterized protein OS=Vitis vinifera GN=VITISV_017186 PE=4 SV=1[more]
A5BFS0_VITVI3.8e-0952.24Putative uncharacterized protein OS=Vitis vinifera GN=VITISV_021511 PE=4 SV=1[more]
A5AMR4_VITVI9.2e-4047.62Putative uncharacterized protein OS=Vitis vinifera GN=VITISV_006313 PE=4 SV=1[more]
BLAST of Cucsa.108530 vs. TAIR10
Match NameE-valueIdentityDescription
AT4G23160.11.2e-1444.29 cysteine-rich RLK (RECEPTOR-like protein kinase) 8[more]
ATMG00820.12.2e-0835.42ATMG00820.1 Reverse transcriptase (RNA-dependent DNA polymerase)[more]
BLAST of Cucsa.108530 vs. NCBI nr
Match NameE-valueIdentityDescription
gi|147819777|emb|CAN76196.1|6.1e-4549.75hypothetical protein VITISV_041073 [Vitis vinifera][more]
gi|147819777|emb|CAN76196.1|8.6e-1559.70hypothetical protein VITISV_041073 [Vitis vinifera][more]
gi|147843633|emb|CAN79872.1|2.5e-1458.21hypothetical protein VITISV_017186 [Vitis vinifera][more]
gi|147790213|emb|CAN65603.1|2.2e-4247.74hypothetical protein VITISV_021511 [Vitis vinifera][more]
gi|147790213|emb|CAN65603.1|5.4e-0952.24hypothetical protein VITISV_021511 [Vitis vinifera][more]
Annotated Terms
The following terms have been associated with this gene:
Vocabulary: INTERPRO
TermDefinition
IPR012337RNaseH-like_sf
IPR013103RVT_2
Vocabulary: Molecular Function
TermDefinition
GO:0003676nucleic acid binding
GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0008150 biological_process
cellular_component GO:0005575 cellular_component
molecular_function GO:0003676 nucleic acid binding
Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Cucsa.108530.1Cucsa.108530.1mRNA


InterPro
Analysis Name: InterPro Annotations of cucumber (Gy14)
Date Performed: 2017-01-17
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR012337Ribonuclease H-like domainunknownSSF53098Ribonuclease H-likecoord: 4..64
score: 1.0
IPR013103Reverse transcriptase, RNA-dependent DNA polymerasePFAMPF07727RVT_2coord: 134..197
score: 2.3
NoneNo IPR availablePANTHERPTHR11439GAG-POL-RELATED RETROTRANSPOSONcoord: 1..280
score: 5.2
NoneNo IPR availablePANTHERPTHR11439:SF185SUBFAMILY NOT NAMEDcoord: 1..280
score: 5.2

Synteny
The following gene(s) are orthologous to this gene:

None

The following gene(s) are paralogous to this gene:

None

The following block(s) are covering this gene:
GeneOrganismBlock
Cucsa.108530Cucurbita pepo (Zucchini)cgycpeB0242
Cucsa.108530Cucurbita pepo (Zucchini)cgycpeB0243
Cucsa.108530Bottle gourd (USVL1VR-Ls)cgylsiB144
Cucsa.108530Melon (DHL92) v3.6.1cgymedB144
Cucsa.108530Silver-seed gourdcarcgyB0216
Cucsa.108530Silver-seed gourdcarcgyB0856
Cucsa.108530Cucumber (Chinese Long) v3cgycucB137
Cucsa.108530Watermelon (97103) v2cgywmbB148
Cucsa.108530Wax gourdcgywgoB171
Cucsa.108530Cucurbita maxima (Rimu)cgycmaB0247
Cucsa.108530Cucurbita maxima (Rimu)cgycmaB0250
Cucsa.108530Cucurbita moschata (Rifu)cgycmoB0246
Cucsa.108530Cucurbita moschata (Rifu)cgycmoB0249
Cucsa.108530Wild cucumber (PI 183967)cgycpiB133
Cucsa.108530Cucumber (Chinese Long) v2cgycuB132
Cucsa.108530Melon (DHL92) v3.5.1cgymeB144
Cucsa.108530Watermelon (Charleston Gray)cgywcgB153
Cucsa.108530Watermelon (97103) v1cgywmB153