Cucsa.105530 (gene) Cucumber (Gy14) v1 | Cucurbit Genomics Database (CuGenDB)

Overview


Name	Cucsa.105530
Type	gene
Organism	Cucumis sativus (Cucumber (Gy14) v1)
Description	Hus1-like protein
Location	scaffold00929 : 168131 .. 169090 (-)

Genome Viewer

Gene Expression

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGAAGTTTAAGGCATTTCTGACGGATAATGGTGTAAATCTCCTTGAGAAGAGGTTTCTACCTGCCCTAGATAAGATGGGGAAGATATGCCATCTCTATTTGACTAGAGATTATGTTATCTTCTTGCATAACCTTCTTAACGGTGATGGAATTCAATCAATTGCTCAGTTTCGTAAAGAAGCTTTGTTTGATGATTATCGAATTTCCAGCCAGAATGATGATCGTATTGCGTTTACTGTAGATATTTCCCTTCTCCACCGCGCAGTTCGTAGTAGTGTTAGTATTTGTTCTGAGTTTGGTAATGGTCCAACGGCAAATCGTCTTCAAATTAAGTTGGTGAAGAAGTTGCCTTTGAATTGCACCCAACCTATGCCTTTCCTTACATTTGAGACTAAAGGGTATAAGTCTGCTGTTATTCAGGATGTGCCGATCTCGAAGCCCATGTCGAGAGCTCAAGTTCTTGAACTTCAAACTGCTCTAGATATGGCACAAGATTTGCCTCAGACGCTTGTGCAGGTTCCTGATCTTAACCAGTTGCAGAACTTTGTGGATCGGATGAAGAATGTTGGGGATTTGTTGAATGTCTCCATAAGCAAGTATGGAGATTTACATGTGCAGATTTCGACAGGGTTGATTACTCTTGGTGCTGAGTATCGAAAATTATTCGTCATAGGGGAACAAGCAAGAGCTCCAGCTGAAGATCAAAATCTAAGTGCTCAGACTAGATCAACCAGGGCGATATTGAGGGGAGATGCTCAATCTGTACAGGTGAGTGTGAAGCACTTTGCTAAGAGTCTTCAGTATCATTTGGCCAAGCCAGATTGCACTTTCTATGGCATTGCTCCACAGGGTGCTTGCTTGACCGTGATATTTCAGTTCTTCATTCCTGGCTCTCGGCAAACTGATAAATCAATCAGTCTACATTGTCGGCTTCCTGTACTTGATCCCGGAACAAGTTAA

mRNA sequence

ATGAAGTTTAAGGCATTTCTGACGGATAATGGTGTAAATCTCCTTGAGAAGAGGTTTCTACCTGCCCTAGATAAGATGGGGAAGATATGCCATCTCTATTTGACTAGAGATTATGTTATCTTCTTGCATAACCTTCTTAACGGTGATGGAATTCAATCAATTGCTCAGTTTCGTAAAGAAGCTTTGTTTGATGATTATCGAATTTCCAGCCAGAATGATGATCGTATTGCGTTTACTGTAGATATTTCCCTTCTCCACCGCGCAGTTCGTAGTAGTGTTAGTATTTGTTCTGAGTTTGGTAATGGTCCAACGGCAAATCGTCTTCAAATTAAGTTGGTGAAGAAGTTGCCTTTGAATTGCACCCAACCTATGCCTTTCCTTACATTTGAGACTAAAGGGTATAAGTCTGCTGTTATTCAGGATGTGCCGATCTCGAAGCCCATGTCGAGAGCTCAAGTTCTTGAACTTCAAACTGCTCTAGATATGGCACAAGATTTGCCTCAGACGCTTGTGCAGGTTCCTGATCTTAACCAGTTGCAGAACTTTGTGGATCGGATGAAGAATGTTGGGGATTTGTTGAATGTCTCCATAAGCAAGTATGGAGATTTACATGTGCAGATTTCGACAGGGTTGATTACTCTTGGTGCTGAGTATCGAAAATTATTCGTCATAGGGGAACAAGCAAGAGCTCCAGCTGAAGATCAAAATCTAAGTGCTCAGACTAGATCAACCAGGGCGATATTGAGGGGAGATGCTCAATCTGTACAGGTGAGTGTGAAGCACTTTGCTAAGAGTCTTCAGTATCATTTGGCCAAGCCAGATTGCACTTTCTATGGCATTGCTCCACAGGGTGCTTGCTTGACCGTGATATTTCAGTTCTTCATTCCTGGCTCTCGGCAAACTGATAAATCAATCAGTCTACATTGTCGGCTTCCTGTACTTGATCCCGGAACAAGTTAA

Coding sequence (CDS)

ATGAAGTTTAAGGCATTTCTGACGGATAATGGTGTAAATCTCCTTGAGAAGAGGTTTCTACCTGCCCTAGATAAGATGGGGAAGATATGCCATCTCTATTTGACTAGAGATTATGTTATCTTCTTGCATAACCTTCTTAACGGTGATGGAATTCAATCAATTGCTCAGTTTCGTAAAGAAGCTTTGTTTGATGATTATCGAATTTCCAGCCAGAATGATGATCGTATTGCGTTTACTGTAGATATTTCCCTTCTCCACCGCGCAGTTCGTAGTAGTGTTAGTATTTGTTCTGAGTTTGGTAATGGTCCAACGGCAAATCGTCTTCAAATTAAGTTGGTGAAGAAGTTGCCTTTGAATTGCACCCAACCTATGCCTTTCCTTACATTTGAGACTAAAGGGTATAAGTCTGCTGTTATTCAGGATGTGCCGATCTCGAAGCCCATGTCGAGAGCTCAAGTTCTTGAACTTCAAACTGCTCTAGATATGGCACAAGATTTGCCTCAGACGCTTGTGCAGGTTCCTGATCTTAACCAGTTGCAGAACTTTGTGGATCGGATGAAGAATGTTGGGGATTTGTTGAATGTCTCCATAAGCAAGTATGGAGATTTACATGTGCAGATTTCGACAGGGTTGATTACTCTTGGTGCTGAGTATCGAAAATTATTCGTCATAGGGGAACAAGCAAGAGCTCCAGCTGAAGATCAAAATCTAAGTGCTCAGACTAGATCAACCAGGGCGATATTGAGGGGAGATGCTCAATCTGTACAGGTGAGTGTGAAGCACTTTGCTAAGAGTCTTCAGTATCATTTGGCCAAGCCAGATTGCACTTTCTATGGCATTGCTCCACAGGGTGCTTGCTTGACCGTGATATTTCAGTTCTTCATTCCTGGCTCTCGGCAAACTGATAAATCAATCAGTCTACATTGTCGGCTTCCTGTACTTGATCCCGGAACAAGTTAA

Protein sequence

MKFKAFLTDNGVNLLEKRFLPALDKMGKICHLYLTRDYVIFLHNLLNGDGIQSIAQFRKEALFDDYRISSQNDDRIAFTVDISLLHRAVRSSVSICSEFGNGPTANRLQIKLVKKLPLNCTQPMPFLTFETKGYKSAVIQDVPISKPMSRAQVLELQTALDMAQDLPQTLVQVPDLNQLQNFVDRMKNVGDLLNVSISKYGDLHVQISTGLITLGAEYRKLFVIGEQARAPAEDQNLSAQTRSTRAILRGDAQSVQVSVKHFAKSLQYHLAKPDCTFYGIAPQGACLTVIFQFFIPGSRQTDKSISLHCRLPVLDPGTS*

Homology

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BLAST of Cucsa.105530 vs. Swiss-Prot
Match: HUS1_DICDI (Checkpoint protein hus1 homolog OS=Dictyostelium discoideum GN=hus1 PE=3 SV=1)

HSP 1 Score: 74.7 bits (182), Expect = 2.0e-12
Identity = 78/283 (27.56%), Postives = 122/283 (43.11%), Query Frame = 1


Query: 1   MKFKAFLTDNGVNLLEKRFLPALDKMGKICHLYLTRDYVIFLHNLLNGDGIQSIAQFRKE 60
           MKFKA ++   + L   + +  + K+   C  ++T D + F+      DG+Q   + ++ 
Sbjct: 1   MKFKAKISKASILL---KTVQNIMKIYNECICHITPDKLRFIIQSDFNDGMQVFCEIQRS 60

Query: 61  ALFDDYRISSQNDDRIAFTVDISLLHRAVRSSVSICSEFGNGPTANRLQIKLVKKLPLNC 120
            +F+++ I S +D+ I F +D+  L R ++S+ S      NG          V  +  N 
Sbjct: 61  LIFENFTIESLSDNEIQFQLDLESLRRVLQSATS------NG----------VSDIFTNL 120

Query: 121 TQPM--PFLTFETKGYKS--AVIQDVPISKPMSRAQVLELQTALDMAQ----DLPQTLVQ 180
           T+    P L F  K   S   + QD+PI           L TA  MAQ     LP  LV 
Sbjct: 121 TKVRGGPVLHFIIKSGTSGTVIFQDIPI----------VLLTAQQMAQINEPTLPDPLVH 180

Query: 181 V--PDLNQLQNFVDRMKNVGDLLNVSISKYGDLHVQISTGLITLGAEYRKLFVIGEQARA 240
           +  P+L  LQ  +D+MKN+ D L + I+    L  ++ T   ++   Y  L         
Sbjct: 181 ILLPNLKNLQKVIDKMKNISDCLKIMIAMNNRLSFEVETSSGSISTFYSGL------DHP 238

Query: 241 PAEDQNLSAQTRSTRAILRGDAQSVQVSVKHFAKSLQYHLAKP 274
              D  LS   ++T          V V +K FAK L  H  KP
Sbjct: 241 QFGDHVLSPDLQAT----------VCVDIKKFAKVLHIHQLKP 238

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BLAST of Cucsa.105530 vs. TrEMBL
Match: A0A0A0K6B2_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_7G432420 PE=4 SV=1)

HSP 1 Score: 632.1 bits (1629), Expect = 3.7e-178
Identity = 319/319 (100.00%), Postives = 319/319 (100.00%), Query Frame = 1


Query: 1   MKFKAFLTDNGVNLLEKRFLPALDKMGKICHLYLTRDYVIFLHNLLNGDGIQSIAQFRKE 60
           MKFKAFLTDNGVNLLEKRFLPALDKMGKICHLYLTRDYVIFLHNLLNGDGIQSIAQFRKE
Sbjct: 1   MKFKAFLTDNGVNLLEKRFLPALDKMGKICHLYLTRDYVIFLHNLLNGDGIQSIAQFRKE 60

Query: 61  ALFDDYRISSQNDDRIAFTVDISLLHRAVRSSVSICSEFGNGPTANRLQIKLVKKLPLNC 120
           ALFDDYRISSQNDDRIAFTVDISLLHRAVRSSVSICSEFGNGPTANRLQIKLVKKLPLNC
Sbjct: 61  ALFDDYRISSQNDDRIAFTVDISLLHRAVRSSVSICSEFGNGPTANRLQIKLVKKLPLNC 120

Query: 121 TQPMPFLTFETKGYKSAVIQDVPISKPMSRAQVLELQTALDMAQDLPQTLVQVPDLNQLQ 180
           TQPMPFLTFETKGYKSAVIQDVPISKPMSRAQVLELQTALDMAQDLPQTLVQVPDLNQLQ
Sbjct: 121 TQPMPFLTFETKGYKSAVIQDVPISKPMSRAQVLELQTALDMAQDLPQTLVQVPDLNQLQ 180

Query: 181 NFVDRMKNVGDLLNVSISKYGDLHVQISTGLITLGAEYRKLFVIGEQARAPAEDQNLSAQ 240
           NFVDRMKNVGDLLNVSISKYGDLHVQISTGLITLGAEYRKLFVIGEQARAPAEDQNLSAQ
Sbjct: 181 NFVDRMKNVGDLLNVSISKYGDLHVQISTGLITLGAEYRKLFVIGEQARAPAEDQNLSAQ 240

Query: 241 TRSTRAILRGDAQSVQVSVKHFAKSLQYHLAKPDCTFYGIAPQGACLTVIFQFFIPGSRQ 300
           TRSTRAILRGDAQSVQVSVKHFAKSLQYHLAKPDCTFYGIAPQGACLTVIFQFFIPGSRQ
Sbjct: 241 TRSTRAILRGDAQSVQVSVKHFAKSLQYHLAKPDCTFYGIAPQGACLTVIFQFFIPGSRQ 300

Query: 301 TDKSISLHCRLPVLDPGTS 320
           TDKSISLHCRLPVLDPGTS
Sbjct: 301 TDKSISLHCRLPVLDPGTS 319

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BLAST of Cucsa.105530 vs. TrEMBL
Match: M5X5T7_PRUPE (Uncharacterized protein OS=Prunus persica GN=PRUPE_ppa008783mg PE=4 SV=1)

HSP 1 Score: 570.5 bits (1469), Expect = 1.3e-159
Identity = 285/318 (89.62%), Postives = 302/318 (94.97%), Query Frame = 1


Query: 1   MKFKAFLTDNGVNLLEKRFLPALDKMGKICHLYLTRDYVIFLHNLLNGDGIQSIAQFRKE 60
           MKFKAFLT+NGVNLLEKRFLPALDKMGKICHLYL RD+ IFLHNLLNGDG+QSIAQFRKE
Sbjct: 1   MKFKAFLTENGVNLLEKRFLPALDKMGKICHLYLNRDHAIFLHNLLNGDGVQSIAQFRKE 60

Query: 61  ALFDDYRISSQNDDRIAFTVDISLLHRAVRSSVSICSEFGNGPTANRLQIKLVKKLPLNC 120
           ALFDDYRISSQN+DRIAF VDISLLHRA+RSSVSIC+EFGNGPTANRLQIKLVKKLP N 
Sbjct: 61  ALFDDYRISSQNEDRIAFAVDISLLHRALRSSVSICTEFGNGPTANRLQIKLVKKLPPNS 120

Query: 121 TQPMPFLTFETKGYKSAVIQDVPISKPMSRAQVLELQTALDMAQDLPQTLVQVPDLNQLQ 180
           TQPMPFLTFETKGYKSAVIQDVPISKPMSRAQVLELQTALDMAQDLPQTLVQVPDLNQLQ
Sbjct: 121 TQPMPFLTFETKGYKSAVIQDVPISKPMSRAQVLELQTALDMAQDLPQTLVQVPDLNQLQ 180

Query: 181 NFVDRMKNVGDLLNVSISKYGDLHVQISTGLITLGAEYRKLFVIGEQARAPAEDQNLSAQ 240
           NFVDRM++VG+LLN+SISKYGDLH+QIST LITLGAE++KL V+GE+A AP+ED NLSAQ
Sbjct: 181 NFVDRMRHVGELLNISISKYGDLHIQISTTLITLGAEFQKLLVLGERADAPSEDLNLSAQ 240

Query: 241 TRSTRAILRGDAQSVQVSVKHFAKSLQYHLAKPDCTFYGIAPQGACLTVIFQFFIPGSRQ 300
           TRS RAILRGDAQSVQVSVKHF KSLQ HLAKPDC FYGIAPQGACLTVIFQFFIPGS Q
Sbjct: 241 TRSARAILRGDAQSVQVSVKHFGKSLQCHLAKPDCAFYGIAPQGACLTVIFQFFIPGSHQ 300

Query: 301 TDKSISLHCRLPVLDPGT 319
            DKSISLHCRLPVLD G+
Sbjct: 301 MDKSISLHCRLPVLDQGS 318

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BLAST of Cucsa.105530 vs. TrEMBL
Match: A0A061G7Q5_THECC (F6D8.25 OS=Theobroma cacao GN=TCM_027268 PE=4 SV=1)

HSP 1 Score: 568.2 bits (1463), Expect = 6.5e-159
Identity = 282/317 (88.96%), Postives = 305/317 (96.21%), Query Frame = 1


Query: 1   MKFKAFLTDNGVNLLEKRFLPALDKMGKICHLYLTRDYVIFLHNLLNGDGIQSIAQFRKE 60
           MKFKAFLT++GV+LLE+RFLPALDKMGKICHL+LTRD+ IFLHNLLNGDG+Q IAQFRKE
Sbjct: 1   MKFKAFLTEHGVSLLERRFLPALDKMGKICHLFLTRDHAIFLHNLLNGDGVQCIAQFRKE 60

Query: 61  ALFDDYRISSQNDDRIAFTVDISLLHRAVRSSVSICSEFGNGPTANRLQIKLVKKLPLNC 120
           ALFDDYRISSQN+DRIAF +D+SLL RAVRSSVSIC+EFGNGP+ANRLQIKLVKKLP NC
Sbjct: 61  ALFDDYRISSQNEDRIAFAIDVSLLLRAVRSSVSICTEFGNGPSANRLQIKLVKKLPPNC 120

Query: 121 TQPMPFLTFETKGYKSAVIQDVPISKPMSRAQVLELQTALDMAQDLPQTLVQVPDLNQLQ 180
           TQPMPFLTFETKGYKSAVIQDVPISKP+SRAQVLELQTALD+AQDLPQTLVQVPDLNQLQ
Sbjct: 121 TQPMPFLTFETKGYKSAVIQDVPISKPLSRAQVLELQTALDIAQDLPQTLVQVPDLNQLQ 180

Query: 181 NFVDRMKNVGDLLNVSISKYGDLHVQISTGLITLGAEYRKLFVIGEQARAPAEDQNLSAQ 240
           NFV+RMK+VGDLLNVSISKYGDLHVQIST LITLGAE+RKL VIGEQA AP+ED+NLSAQ
Sbjct: 181 NFVERMKHVGDLLNVSISKYGDLHVQISTTLITLGAEFRKLLVIGEQAEAPSEDRNLSAQ 240

Query: 241 TRSTRAILRGDAQSVQVSVKHFAKSLQYHLAKPDCTFYGIAPQGACLTVIFQFFIPGSRQ 300
           +RS RAI RGDAQSVQVSVKHF++SLQ HLAKPDC FYGIAPQGACLTVIFQFFIPG+RQ
Sbjct: 241 SRSERAISRGDAQSVQVSVKHFSRSLQCHLAKPDCAFYGIAPQGACLTVIFQFFIPGTRQ 300

Query: 301 TDKSISLHCRLPVLDPG 318
           TDKSISLHCRLPVLDPG
Sbjct: 301 TDKSISLHCRLPVLDPG 317

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BLAST of Cucsa.105530 vs. TrEMBL
Match: A0A0D2UE99_GOSRA (Uncharacterized protein OS=Gossypium raimondii GN=B456_010G138400 PE=4 SV=1)

HSP 1 Score: 559.3 bits (1440), Expect = 3.0e-156
Identity = 276/319 (86.52%), Postives = 302/319 (94.67%), Query Frame = 1


Query: 1   MKFKAFLTDNGVNLLEKRFLPALDKMGKICHLYLTRDYVIFLHNLLNGDGIQSIAQFRKE 60
           MKFKAFLT++GVNLL +RFLPALDKMGKICHL+LTRD+ IFLHNLLNGDG+Q IAQFRKE
Sbjct: 1   MKFKAFLTEHGVNLLGRRFLPALDKMGKICHLFLTRDHAIFLHNLLNGDGVQCIAQFRKE 60

Query: 61  ALFDDYRISSQNDDRIAFTVDISLLHRAVRSSVSICSEFGNGPTANRLQIKLVKKLPLNC 120
           ALFDDYRISSQN+DRIAF +D+SLL RAVRSSVSIC+EFGNGP+ANRLQIKLVKKLPLNC
Sbjct: 61  ALFDDYRISSQNEDRIAFAIDVSLLLRAVRSSVSICTEFGNGPSANRLQIKLVKKLPLNC 120

Query: 121 TQPMPFLTFETKGYKSAVIQDVPISKPMSRAQVLELQTALDMAQDLPQTLVQVPDLNQLQ 180
           TQPMPFLTFETKGYKSAVIQDVPISKP+SRAQVLELQTAL++AQD+PQTLV+VPDLNQLQ
Sbjct: 121 TQPMPFLTFETKGYKSAVIQDVPISKPLSRAQVLELQTALELAQDIPQTLVRVPDLNQLQ 180

Query: 181 NFVDRMKNVGDLLNVSISKYGDLHVQISTGLITLGAEYRKLFVIGEQARAPAEDQNLSAQ 240
           NFV+RMK VGDLLNVSISKYGDLHVQIST LITLGAE+RKL VIGEQA AP++D+N SAQ
Sbjct: 181 NFVERMKQVGDLLNVSISKYGDLHVQISTTLITLGAEFRKLLVIGEQAEAPSDDRNQSAQ 240

Query: 241 TRSTRAILRGDAQSVQVSVKHFAKSLQYHLAKPDCTFYGIAPQGACLTVIFQFFIPGSRQ 300
           TRS RAI RGDAQ VQVSVKHF++SL  HLAKPDCTFYGIAP G+CLTVIFQFFIPG+ Q
Sbjct: 241 TRSERAISRGDAQRVQVSVKHFSRSLHCHLAKPDCTFYGIAPHGSCLTVIFQFFIPGTHQ 300

Query: 301 TDKSISLHCRLPVLDPGTS 320
           TDKSISLHCRLPVLDPG+S
Sbjct: 301 TDKSISLHCRLPVLDPGSS 319

[Close]

BLAST of Cucsa.105530 vs. TrEMBL
Match: W9RC19_9ROSA (Uncharacterized protein OS=Morus notabilis GN=L484_025745 PE=4 SV=1)

HSP 1 Score: 558.9 bits (1439), Expect = 4.0e-156
Identity = 276/319 (86.52%), Postives = 302/319 (94.67%), Query Frame = 1


Query: 1   MKFKAFLTDNGVNLLEKRFLPALDKMGKICHLYLTRDYVIFLHNLLNGDGIQSIAQFRKE 60
           MKFKAFLT+NGVNLLEKRFLPALD+MGKICHLYLTRD+ IFLHNLLNGDGIQS+AQFR+E
Sbjct: 1   MKFKAFLTENGVNLLEKRFLPALDRMGKICHLYLTRDHAIFLHNLLNGDGIQSVAQFRRE 60

Query: 61  ALFDDYRISSQNDDRIAFTVDISLLHRAVRSSVSICSEFGNGPTANRLQIKLVKKLPLNC 120
           ALFDDYRISSQN+DRIAF VD+SLLHRA+RSSVSIC+EFG+GPTANRL IKLVKKLP N 
Sbjct: 61  ALFDDYRISSQNEDRIAFAVDVSLLHRALRSSVSICTEFGSGPTANRLHIKLVKKLPANS 120

Query: 121 TQPMPFLTFETKGYKSAVIQDVPISKPMSRAQVLELQTALDMAQDLPQTLVQVPDLNQLQ 180
           +QPMPFLTFETKG+KSAVIQDVPISKP+SRA +LELQTALD AQDLP TLVQVPDLNQLQ
Sbjct: 121 SQPMPFLTFETKGFKSAVIQDVPISKPLSRAHLLELQTALDAAQDLPPTLVQVPDLNQLQ 180

Query: 181 NFVDRMKNVGDLLNVSISKYGDLHVQISTGLITLGAEYRKLFVIGEQARAPAEDQNLSAQ 240
           +FVDRMK++G+LLNVSI+KYG+LH+QIST  ITLGAE+R+L VIGEQA AP ED+NLSAQ
Sbjct: 181 SFVDRMKHIGELLNVSITKYGELHMQISTTFITLGAEFRQLLVIGEQADAPVEDRNLSAQ 240

Query: 241 TRSTRAILRGDAQSVQVSVKHFAKSLQYHLAKPDCTFYGIAPQGACLTVIFQFFIPGSRQ 300
            RS+RAILRGDAQSVQVSVKHFAKSL YHLAKPDC FYGIAPQGACLTVIFQFFIPGSRQ
Sbjct: 241 ARSSRAILRGDAQSVQVSVKHFAKSLCYHLAKPDCAFYGIAPQGACLTVIFQFFIPGSRQ 300

Query: 301 TDKSISLHCRLPVLDPGTS 320
            DKSISLHCRLPVLDPG+S
Sbjct: 301 MDKSISLHCRLPVLDPGSS 319

[Close]

BLAST of Cucsa.105530 vs. TAIR10
Match: AT1G52530.1 (AT1G52530.1 Hus1-like protein (InterPro:IPR007150))

HSP 1 Score: 514.6 bits (1324), Expect = 4.3e-146
Identity = 252/319 (79.00%), Postives = 290/319 (90.91%), Query Frame = 1


Query: 1   MKFKAFLTDNGVNLLEKRFLPALDKMGKICHLYLTRDYVIFLHNLLNGDGIQSIAQFRKE 60
           MKFKAFLT+NGVNLLEKRFLPA DKMGK CHL LT++++ FLHNLLNGDG+Q IAQFRK+
Sbjct: 1   MKFKAFLTENGVNLLEKRFLPAFDKMGKNCHLLLTKEHLFFLHNLLNGDGVQCIAQFRKD 60

Query: 61  ALFDDYRISSQNDDRIAFTVDISLLHRAVRSSVSICSEFGNGPTANRLQIKLVKKLPLNC 120
            LFDDYRISSQN+DRIAF++D++LL+RAV+SSVSIC+EF  G  +NRLQIKLVKKLP NC
Sbjct: 61  VLFDDYRISSQNEDRIAFSLDVALLYRAVKSSVSICTEFSGGLASNRLQIKLVKKLPPNC 120

Query: 121 TQPMPFLTFETKGYKSAVIQDVPISKPMSRAQVLELQTALDMAQDLPQTLVQVPDLNQLQ 180
           TQPMPFLTFETKGYKSAVIQDVPI+KP+SR+QV+ELQTALD AQDLP TLVQV D NQLQ
Sbjct: 121 TQPMPFLTFETKGYKSAVIQDVPITKPLSRSQVIELQTALDSAQDLPPTLVQVQDSNQLQ 180

Query: 181 NFVDRMKNVGDLLNVSISKYGDLHVQISTGLITLGAEYRKLFVIGEQARAPAEDQNLSAQ 240
           NFVD M++VGD+LNV+ISK+GDL VQ+ST LI LG E+++L VIGE+++AP ED+NLSAQ
Sbjct: 181 NFVDHMRHVGDVLNVTISKHGDLQVQVSTTLIRLGIEFQRLSVIGEKSQAPVEDRNLSAQ 240

Query: 241 TRSTRAILRGDAQSVQVSVKHFAKSLQYHLAKPDCTFYGIAPQGACLTVIFQFFIPGSRQ 300
            RS RAI RGDAQSVQVSVKHF+KSLQ HL KPD TFYGIAPQGACLTVIFQF +PG+RQ
Sbjct: 241 ARSERAIARGDAQSVQVSVKHFSKSLQCHLTKPDSTFYGIAPQGACLTVIFQFMVPGTRQ 300

Query: 301 TDKSISLHCRLPVLDPGTS 320
           TDKSISLHCRLPVLDPG++
Sbjct: 301 TDKSISLHCRLPVLDPGSN 319

[Close]

BLAST of Cucsa.105530 vs. NCBI nr
Match: gi|449436140|ref|XP_004135852.1| (PREDICTED: uncharacterized protein LOC101207808 [Cucumis sativus])

HSP 1 Score: 632.1 bits (1629), Expect = 5.3e-178
Identity = 319/319 (100.00%), Postives = 319/319 (100.00%), Query Frame = 1


Query: 1   MKFKAFLTDNGVNLLEKRFLPALDKMGKICHLYLTRDYVIFLHNLLNGDGIQSIAQFRKE 60
           MKFKAFLTDNGVNLLEKRFLPALDKMGKICHLYLTRDYVIFLHNLLNGDGIQSIAQFRKE
Sbjct: 1   MKFKAFLTDNGVNLLEKRFLPALDKMGKICHLYLTRDYVIFLHNLLNGDGIQSIAQFRKE 60

Query: 61  ALFDDYRISSQNDDRIAFTVDISLLHRAVRSSVSICSEFGNGPTANRLQIKLVKKLPLNC 120
           ALFDDYRISSQNDDRIAFTVDISLLHRAVRSSVSICSEFGNGPTANRLQIKLVKKLPLNC
Sbjct: 61  ALFDDYRISSQNDDRIAFTVDISLLHRAVRSSVSICSEFGNGPTANRLQIKLVKKLPLNC 120

Query: 121 TQPMPFLTFETKGYKSAVIQDVPISKPMSRAQVLELQTALDMAQDLPQTLVQVPDLNQLQ 180
           TQPMPFLTFETKGYKSAVIQDVPISKPMSRAQVLELQTALDMAQDLPQTLVQVPDLNQLQ
Sbjct: 121 TQPMPFLTFETKGYKSAVIQDVPISKPMSRAQVLELQTALDMAQDLPQTLVQVPDLNQLQ 180

Query: 181 NFVDRMKNVGDLLNVSISKYGDLHVQISTGLITLGAEYRKLFVIGEQARAPAEDQNLSAQ 240
           NFVDRMKNVGDLLNVSISKYGDLHVQISTGLITLGAEYRKLFVIGEQARAPAEDQNLSAQ
Sbjct: 181 NFVDRMKNVGDLLNVSISKYGDLHVQISTGLITLGAEYRKLFVIGEQARAPAEDQNLSAQ 240

Query: 241 TRSTRAILRGDAQSVQVSVKHFAKSLQYHLAKPDCTFYGIAPQGACLTVIFQFFIPGSRQ 300
           TRSTRAILRGDAQSVQVSVKHFAKSLQYHLAKPDCTFYGIAPQGACLTVIFQFFIPGSRQ
Sbjct: 241 TRSTRAILRGDAQSVQVSVKHFAKSLQYHLAKPDCTFYGIAPQGACLTVIFQFFIPGSRQ 300

Query: 301 TDKSISLHCRLPVLDPGTS 320
           TDKSISLHCRLPVLDPGTS
Sbjct: 301 TDKSISLHCRLPVLDPGTS 319

[Close]

BLAST of Cucsa.105530 vs. NCBI nr
Match: gi|659122415|ref|XP_008461131.1| (PREDICTED: uncharacterized protein LOC103499810 [Cucumis melo])

HSP 1 Score: 626.3 bits (1614), Expect = 2.9e-176
Identity = 316/319 (99.06%), Postives = 317/319 (99.37%), Query Frame = 1


Query: 1   MKFKAFLTDNGVNLLEKRFLPALDKMGKICHLYLTRDYVIFLHNLLNGDGIQSIAQFRKE 60
           MKFKAFLTDNGVNLLEKRFLPALDKMGKICHLYLTRDYVIFLHNLLNGDGIQSIAQFRKE
Sbjct: 1   MKFKAFLTDNGVNLLEKRFLPALDKMGKICHLYLTRDYVIFLHNLLNGDGIQSIAQFRKE 60

Query: 61  ALFDDYRISSQNDDRIAFTVDISLLHRAVRSSVSICSEFGNGPTANRLQIKLVKKLPLNC 120
           ALFDDYRISSQNDDRIAFT+DISLLHRAVRSSVSICSEFGNGPTANRLQIKLVKKLPLNC
Sbjct: 61  ALFDDYRISSQNDDRIAFTIDISLLHRAVRSSVSICSEFGNGPTANRLQIKLVKKLPLNC 120

Query: 121 TQPMPFLTFETKGYKSAVIQDVPISKPMSRAQVLELQTALDMAQDLPQTLVQVPDLNQLQ 180
           TQ MPFLTFETKGYKSAVIQDVPISKPMSRAQVLELQTALDMAQDLPQTLVQVPDLNQLQ
Sbjct: 121 TQSMPFLTFETKGYKSAVIQDVPISKPMSRAQVLELQTALDMAQDLPQTLVQVPDLNQLQ 180

Query: 181 NFVDRMKNVGDLLNVSISKYGDLHVQISTGLITLGAEYRKLFVIGEQARAPAEDQNLSAQ 240
           NFVDRMKNVGDLLNVSISKYGDLHVQISTGLITLGAEYRKLFVIGEQA APAEDQNLSAQ
Sbjct: 181 NFVDRMKNVGDLLNVSISKYGDLHVQISTGLITLGAEYRKLFVIGEQASAPAEDQNLSAQ 240

Query: 241 TRSTRAILRGDAQSVQVSVKHFAKSLQYHLAKPDCTFYGIAPQGACLTVIFQFFIPGSRQ 300
           TRSTRAILRGDAQSVQVSVKHFAKSLQYHLAKPDCTFYGIAPQGACLTVIFQFFIPGSRQ
Sbjct: 241 TRSTRAILRGDAQSVQVSVKHFAKSLQYHLAKPDCTFYGIAPQGACLTVIFQFFIPGSRQ 300

Query: 301 TDKSISLHCRLPVLDPGTS 320
           TDKSISLHCRLPVLDPGTS
Sbjct: 301 TDKSISLHCRLPVLDPGTS 319

[Close]

BLAST of Cucsa.105530 vs. NCBI nr
Match: gi|595938775|ref|XP_007215694.1| (hypothetical protein PRUPE_ppa008783mg [Prunus persica])

HSP 1 Score: 570.5 bits (1469), Expect = 1.9e-159
Identity = 285/318 (89.62%), Postives = 302/318 (94.97%), Query Frame = 1


Query: 1   MKFKAFLTDNGVNLLEKRFLPALDKMGKICHLYLTRDYVIFLHNLLNGDGIQSIAQFRKE 60
           MKFKAFLT+NGVNLLEKRFLPALDKMGKICHLYL RD+ IFLHNLLNGDG+QSIAQFRKE
Sbjct: 1   MKFKAFLTENGVNLLEKRFLPALDKMGKICHLYLNRDHAIFLHNLLNGDGVQSIAQFRKE 60

Query: 61  ALFDDYRISSQNDDRIAFTVDISLLHRAVRSSVSICSEFGNGPTANRLQIKLVKKLPLNC 120
           ALFDDYRISSQN+DRIAF VDISLLHRA+RSSVSIC+EFGNGPTANRLQIKLVKKLP N 
Sbjct: 61  ALFDDYRISSQNEDRIAFAVDISLLHRALRSSVSICTEFGNGPTANRLQIKLVKKLPPNS 120

Query: 121 TQPMPFLTFETKGYKSAVIQDVPISKPMSRAQVLELQTALDMAQDLPQTLVQVPDLNQLQ 180
           TQPMPFLTFETKGYKSAVIQDVPISKPMSRAQVLELQTALDMAQDLPQTLVQVPDLNQLQ
Sbjct: 121 TQPMPFLTFETKGYKSAVIQDVPISKPMSRAQVLELQTALDMAQDLPQTLVQVPDLNQLQ 180

Query: 181 NFVDRMKNVGDLLNVSISKYGDLHVQISTGLITLGAEYRKLFVIGEQARAPAEDQNLSAQ 240
           NFVDRM++VG+LLN+SISKYGDLH+QIST LITLGAE++KL V+GE+A AP+ED NLSAQ
Sbjct: 181 NFVDRMRHVGELLNISISKYGDLHIQISTTLITLGAEFQKLLVLGERADAPSEDLNLSAQ 240

Query: 241 TRSTRAILRGDAQSVQVSVKHFAKSLQYHLAKPDCTFYGIAPQGACLTVIFQFFIPGSRQ 300
           TRS RAILRGDAQSVQVSVKHF KSLQ HLAKPDC FYGIAPQGACLTVIFQFFIPGS Q
Sbjct: 241 TRSARAILRGDAQSVQVSVKHFGKSLQCHLAKPDCAFYGIAPQGACLTVIFQFFIPGSHQ 300

Query: 301 TDKSISLHCRLPVLDPGT 319
            DKSISLHCRLPVLD G+
Sbjct: 301 MDKSISLHCRLPVLDQGS 318

[Close]

BLAST of Cucsa.105530 vs. NCBI nr
Match: gi|645245090|ref|XP_008228717.1| (PREDICTED: uncharacterized protein LOC103328104 [Prunus mume])

HSP 1 Score: 569.3 bits (1466), Expect = 4.2e-159
Identity = 283/318 (88.99%), Postives = 303/318 (95.28%), Query Frame = 1


Query: 1   MKFKAFLTDNGVNLLEKRFLPALDKMGKICHLYLTRDYVIFLHNLLNGDGIQSIAQFRKE 60
           MKFKAFLT+NGVNLLEKRFLPALDKMGKICHLYL RD+ IFLHNLLNGDG+QSIAQFRKE
Sbjct: 1   MKFKAFLTENGVNLLEKRFLPALDKMGKICHLYLNRDHAIFLHNLLNGDGVQSIAQFRKE 60

Query: 61  ALFDDYRISSQNDDRIAFTVDISLLHRAVRSSVSICSEFGNGPTANRLQIKLVKKLPLNC 120
           ALFDDYRISSQN+DRIAF+VDISLLHRA+RSSVSIC+EFGNGPTANRLQIKLVKKLP N 
Sbjct: 61  ALFDDYRISSQNEDRIAFSVDISLLHRALRSSVSICTEFGNGPTANRLQIKLVKKLPPNS 120

Query: 121 TQPMPFLTFETKGYKSAVIQDVPISKPMSRAQVLELQTALDMAQDLPQTLVQVPDLNQLQ 180
           TQPMPFLTFETKGYKSAVIQDVPISKP+SRAQVLELQTALDMAQDLPQTLVQ+PDLNQLQ
Sbjct: 121 TQPMPFLTFETKGYKSAVIQDVPISKPLSRAQVLELQTALDMAQDLPQTLVQIPDLNQLQ 180

Query: 181 NFVDRMKNVGDLLNVSISKYGDLHVQISTGLITLGAEYRKLFVIGEQARAPAEDQNLSAQ 240
           NFVDRM++VG+LLN+SISKYGDLH+QIST LITLGAE++KL V+GE+A AP+ED NLSAQ
Sbjct: 181 NFVDRMRHVGELLNISISKYGDLHIQISTTLITLGAEFQKLLVLGERADAPSEDGNLSAQ 240

Query: 241 TRSTRAILRGDAQSVQVSVKHFAKSLQYHLAKPDCTFYGIAPQGACLTVIFQFFIPGSRQ 300
           TRS RAILRGDAQSVQVSVKHF KSLQ HLAKPDC FYGIAPQGACLTVIFQFFIPGS Q
Sbjct: 241 TRSARAILRGDAQSVQVSVKHFGKSLQCHLAKPDCAFYGIAPQGACLTVIFQFFIPGSHQ 300

Query: 301 TDKSISLHCRLPVLDPGT 319
            DKSISLHCRLPVLD G+
Sbjct: 301 MDKSISLHCRLPVLDQGS 318

[Close]

BLAST of Cucsa.105530 vs. NCBI nr
Match: gi|590615636|ref|XP_007023278.1| (F6D8.25 [Theobroma cacao])

HSP 1 Score: 568.2 bits (1463), Expect = 9.4e-159
Identity = 282/317 (88.96%), Postives = 305/317 (96.21%), Query Frame = 1


Query: 1   MKFKAFLTDNGVNLLEKRFLPALDKMGKICHLYLTRDYVIFLHNLLNGDGIQSIAQFRKE 60
           MKFKAFLT++GV+LLE+RFLPALDKMGKICHL+LTRD+ IFLHNLLNGDG+Q IAQFRKE
Sbjct: 1   MKFKAFLTEHGVSLLERRFLPALDKMGKICHLFLTRDHAIFLHNLLNGDGVQCIAQFRKE 60

Query: 61  ALFDDYRISSQNDDRIAFTVDISLLHRAVRSSVSICSEFGNGPTANRLQIKLVKKLPLNC 120
           ALFDDYRISSQN+DRIAF +D+SLL RAVRSSVSIC+EFGNGP+ANRLQIKLVKKLP NC
Sbjct: 61  ALFDDYRISSQNEDRIAFAIDVSLLLRAVRSSVSICTEFGNGPSANRLQIKLVKKLPPNC 120

Query: 121 TQPMPFLTFETKGYKSAVIQDVPISKPMSRAQVLELQTALDMAQDLPQTLVQVPDLNQLQ 180
           TQPMPFLTFETKGYKSAVIQDVPISKP+SRAQVLELQTALD+AQDLPQTLVQVPDLNQLQ
Sbjct: 121 TQPMPFLTFETKGYKSAVIQDVPISKPLSRAQVLELQTALDIAQDLPQTLVQVPDLNQLQ 180

Query: 181 NFVDRMKNVGDLLNVSISKYGDLHVQISTGLITLGAEYRKLFVIGEQARAPAEDQNLSAQ 240
           NFV+RMK+VGDLLNVSISKYGDLHVQIST LITLGAE+RKL VIGEQA AP+ED+NLSAQ
Sbjct: 181 NFVERMKHVGDLLNVSISKYGDLHVQISTTLITLGAEFRKLLVIGEQAEAPSEDRNLSAQ 240

Query: 241 TRSTRAILRGDAQSVQVSVKHFAKSLQYHLAKPDCTFYGIAPQGACLTVIFQFFIPGSRQ 300
           +RS RAI RGDAQSVQVSVKHF++SLQ HLAKPDC FYGIAPQGACLTVIFQFFIPG+RQ
Sbjct: 241 SRSERAISRGDAQSVQVSVKHFSRSLQCHLAKPDCAFYGIAPQGACLTVIFQFFIPGTRQ 300

Query: 301 TDKSISLHCRLPVLDPG 318
           TDKSISLHCRLPVLDPG
Sbjct: 301 TDKSISLHCRLPVLDPG 317

The following BLAST results are available for this feature:

BLAST of Cucsa.105530 vs. Swiss-Prot


Match Name	E-value	Identity	Description
HUS1_DICDI	2.0e-12	27.56	Checkpoint protein hus1 homolog OS=Dictyostelium discoideum GN=hus1 PE=3 SV=1	[more]

BLAST of Cucsa.105530 vs. TrEMBL


Match Name	E-value	Identity	Description
A0A0A0K6B2_CUCSA	3.7e-178	100.00	Uncharacterized protein OS=Cucumis sativus GN=Csa_7G432420 PE=4 SV=1	[more]
M5X5T7_PRUPE	1.3e-159	89.62	Uncharacterized protein OS=Prunus persica GN=PRUPE_ppa008783mg PE=4 SV=1	[more]
A0A061G7Q5_THECC	6.5e-159	88.96	F6D8.25 OS=Theobroma cacao GN=TCM_027268 PE=4 SV=1	[more]
A0A0D2UE99_GOSRA	3.0e-156	86.52	Uncharacterized protein OS=Gossypium raimondii GN=B456_010G138400 PE=4 SV=1	[more]
W9RC19_9ROSA	4.0e-156	86.52	Uncharacterized protein OS=Morus notabilis GN=L484_025745 PE=4 SV=1	[more]

BLAST of Cucsa.105530 vs. TAIR10


Match Name	E-value	Identity	Description
AT1G52530.1	4.3e-146	79.00	Hus1-like protein (InterPro:IPR007150)	[more]

BLAST of Cucsa.105530 vs. NCBI nr


Match Name	E-value	Identity	Description
gi\|449436140\|ref\|XP_004135852.1\|	5.3e-178	100.00	PREDICTED: uncharacterized protein LOC101207808 [Cucumis sativus]	[more]
gi\|659122415\|ref\|XP_008461131.1\|	2.9e-176	99.06	PREDICTED: uncharacterized protein LOC103499810 [Cucumis melo]	[more]
gi\|595938775\|ref\|XP_007215694.1\|	1.9e-159	89.62	hypothetical protein PRUPE_ppa008783mg [Prunus persica]	[more]
gi\|645245090\|ref\|XP_008228717.1\|	4.2e-159	88.99	PREDICTED: uncharacterized protein LOC103328104 [Prunus mume]	[more]
gi\|590615636\|ref\|XP_007023278.1\|	9.4e-159	88.96	F6D8.25 [Theobroma cacao]	[more]

Annotated Terms

The following terms have been associated with this gene:

Vocabulary: **INTERPRO**
Term	Definition
IPR007150	Hus1/Mec3
IPR016580	Cell_cycle_HUS1

Vocabulary: **Biological Process**
Term	Definition
GO:0000077	DNA damage checkpoint
GO:0006281	DNA repair

Vocabulary: **Cellular Component**
Term	Definition
GO:0030896	checkpoint clamp complex
GO:0005730	nucleolus

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0000077	DNA damage checkpoint
biological_process	GO:0006281	DNA repair
cellular_component	GO:0030896	checkpoint clamp complex
cellular_component	GO:0005730	nucleolus
molecular_function	GO:0003674	molecular_function

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Cucsa.105530.1	Cucsa.105530.1	mRNA

InterPro

Analysis Name: InterPro Annotations of cucumber (Gy14)
Date Performed: 2017-01-17


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR007150	Checkpoint protein Hus1/Mec3	PFAM	PF04005	Hus1	coord: 1..298 score: 1.1
IPR016580	Cell cycle checkpoint, Hus1	PIR	PIRSF011312	HUS1	coord: 1..299 score: 1.1
None	No IPR available	PANTHER	PTHR12900	MITOTIC AND DNA DAMAGE CHECKPOINT PROTEIN HUS1	coord: 2..315 score: 4.4E
None	No IPR available	PANTHER	PTHR12900:SF0	HUS1-LIKE	coord: 2..315 score: 4.4E

Synteny

The following gene(s) are orthologous to this gene:


Gene	Orthologue	Organism	Block
Cucsa.105530	Cla015351	Watermelon (97103) v1	cgywmB142
Cucsa.105530	Csa7G432420	Cucumber (Chinese Long) v2	cgycuB126
Cucsa.105530	MELO3C023430	Melon (DHL92) v3.5.1	cgymeB133
Cucsa.105530	CSPI07G20460	Wild cucumber (PI 183967)	cgycpiB127
Cucsa.105530	CmaCh17G008690	Cucurbita maxima (Rimu)	cgycmaB0237
Cucsa.105530	CmaCh08G006130	Cucurbita maxima (Rimu)	cgycmaB0241
Cucsa.105530	CmoCh08G006030	Cucurbita moschata (Rifu)	cgycmoB0240
Cucsa.105530	CmoCh17G008390	Cucurbita moschata (Rifu)	cgycmoB0236
Cucsa.105530	Lsi02G027020	Bottle gourd (USVL1VR-Ls)	cgylsiB133
Cucsa.105530	Cp4.1LG17g08730	Cucurbita pepo (Zucchini)	cgycpeB0230
Cucsa.105530	MELO3C023430.2	Melon (DHL92) v3.6.1	cgymedB133
Cucsa.105530	CsaV3_7G032300	Cucumber (Chinese Long) v3	cgycucB131
Cucsa.105530	Cla97C09G165210	Watermelon (97103) v2	cgywmbB141
Cucsa.105530	Bhi09G000008	Wax gourd	cgywgoB162
Cucsa.105530	Carg23136	Silver-seed gourd	carcgyB0179
Cucsa.105530	Carg09593	Silver-seed gourd	carcgyB1144

The following gene(s) are paralogous to this gene:

None

The following block(s) are covering this gene:


Gene	Organism	Block
Cucsa.105530	Cucurbita pepo (Zucchini)	cgycpeB0229
Cucsa.105530	Cucurbita pepo (Zucchini)	cgycpeB0234
Cucsa.105530	Bottle gourd (USVL1VR-Ls)	cgylsiB134
Cucsa.105530	Bottle gourd (USVL1VR-Ls)	cgylsiB136
Cucsa.105530	Melon (DHL92) v3.6.1	cgymedB135
Cucsa.105530	Silver-seed gourd	carcgyB0238
Cucsa.105530	Cucumber (Chinese Long) v3	cgycucB129
Cucsa.105530	Watermelon (97103) v2	cgywmbB138
Cucsa.105530	Cucumber (Gy14) v1	cgycgyB032
Cucsa.105530	Cucumber (Gy14) v1	cgycgyB053
Cucsa.105530	Cucurbita maxima (Rimu)	cgycmaB0238
Cucsa.105530	Cucurbita maxima (Rimu)	cgycmaB0239
Cucsa.105530	Cucurbita moschata (Rifu)	cgycmoB0235
Cucsa.105530	Wild cucumber (PI 183967)	cgycpiB124
Cucsa.105530	Wild cucumber (PI 183967)	cgycpiB125
Cucsa.105530	Cucumber (Chinese Long) v2	cgycuB123
Cucsa.105530	Cucumber (Chinese Long) v2	cgycuB124
Cucsa.105530	Melon (DHL92) v3.5.1	cgymeB135
Cucsa.105530	Watermelon (Charleston Gray)	cgywcgB141
Cucsa.105530	Watermelon (Charleston Gray)	cgywcgB143
Cucsa.105530	Watermelon (97103) v1	cgywmB143
Cucsa.105530	Watermelon (97103) v1	cgywmB144