Cucsa.104990 (gene) Cucumber (Gy14) v1

BLAST of Cucsa.104990 vs. Swiss-Prot
Match: VCL21_ARATH (Vicilin-like seed storage protein At2g18540 OS=Arabidopsis thaliana GN=At2g18540 PE=3 SV=1)

HSP 1 Score: 381.3 bits (978), Expect = 1.5e-104
Identity = 205/426 (48.12%), Postives = 286/426 (67.14%), Query Frame = 1

Query: 42  TPVVKRANERIPILKTEYGEISAVDFDDGTRFGRYHLQFITLEPNSLFLPVLLHSDMVFY 101
           +P++ + ++R  ++ TE+G ISAV   DG     YH+QFITLEPN+L LP+LLHSDMVF+
Sbjct: 41  SPLLVKKDQRTSVVATEFGNISAVQIGDG-----YHIQFITLEPNALLLPLLLHSDMVFF 100

Query: 102 VHTGSGRLNWFDDNDLKEVDLRRGDLYRLHPGSIFYLQSSLETEREKLRIYALFSSTDED 161
           VHTG+G LNW D+   ++++LRRGD++RL  G++FY+ S+     EKLR+YA+F+   + 
Sbjct: 101 VHTGTGILNWIDEESERKLELRRGDVFRLRSGTVFYVHSN-----EKLRVYAIFN-VGKC 160

Query: 162 SFNPSIGAYSRVTDLVRGFGKEVLRKAFMAPDEVIEEIMNAKRPPLIVHAAAPTPSIKAK 221
             +P +GAYS V DL+ GF    LR AF  P++++ +I +A +PPLIV+A    P  + +
Sbjct: 161 LNDPCLGAYSSVRDLLLGFDDRTLRSAFAVPEDILRKIRDATKPPLIVNAL---PRNRTQ 220

Query: 222 SSSPWEFEARLLKSFLGGDASAIEFNKK------KKKKGIYNVYEVDPDFENCNGWSLTV 281
                ++++RL++ F+  +        K      KKK   +NV+E DPDFEN NG S+ V
Sbjct: 221 GLEEDKWQSRLVRLFVSVEDVTDHLAMKPIVDTNKKKSRTFNVFEEDPDFENNNGRSIVV 280

Query: 282 TKKNSHQLKGSNIGFLVVNLTAGSMMGPHWNPRAWEIGIVTSDEPGVIRVGCSSTSANSS 341
            +K+   LKGS  G  +VNLT GSM+GPHWNP A EI IV   E  V  V   S S+  +
Sbjct: 281 DEKDLDALKGSRFGVFMVNLTKGSMIGPHWNPSACEISIVLEGEGMVRVVNQQSLSSCKN 340

Query: 342 KCKNWSFVVEKGDVFVVPRFHPMAQMSFNNGTFVFVGFSTTNGHNMPQFFAGSSSVLKIV 401
             K+ SF+VE+GDVFVVP+FHPMAQMSF N +FVF+GFST+   N PQF  G SSVLK++
Sbjct: 341 DRKSESFMVEEGDVFVVPKFHPMAQMSFENSSFVFMGFSTSAKTNHPQFLVGQSSVLKVL 400

Query: 402 DREVLAWSFDVNVTTIDRLLKARVESIVLECTSCAEEE----VRKMEEEAEREREEEEER 458
           DR+V+A SF+++  TI  LLKA+ ES++ EC SCAE E    +R++EE   RE EE E R
Sbjct: 401 DRDVVAVSFNLSNETIKGLLKAQKESVIFECASCAEGELSKLMREIEERKRREEEEIERR 452

BLAST of Cucsa.104990 vs. Swiss-Prot
Match: VCL43_ARATH (Vicilin-like seed storage protein At4g36700 OS=Arabidopsis thaliana GN=At4g36700 PE=3 SV=1)

HSP 1 Score: 380.2 bits (975), Expect = 3.2e-104
Identity = 214/453 (47.24%), Postives = 294/453 (64.90%), Query Frame = 1

Query: 42  TPVVKRANERIPILKTEYGEISAVDFDDGTR-FGRYHLQFITLEPNSLFLPVLLHSDMVF 101
           +P++ + ++  PI +T++G+IS V   +G    G Y +  ITLEPN++ LP+LLHSDMVF
Sbjct: 45  SPLLIKKDQWKPIFETKFGQISTVQIGNGCGGMGPYKIHSITLEPNTILLPLLLHSDMVF 104

Query: 102 YVHTGSGRLNWFDDNDLKEVDLRRGDLYRLHPGSIFYLQSS-----LETEREKLRIYALF 161
           +V +GSG LNW D+ + K  ++R GD+YRL PGS+FYLQS      L T   KL++YA+F
Sbjct: 105 FVDSGSGILNWVDE-EAKSTEIRLGDVYRLRPGSVFYLQSKPVDIFLGT---KLKLYAIF 164

Query: 162 SSTDEDSFNPSIGAYSRVTDLVRGFGKEVLRKAFMAPDEVIEEIMNAKRPPLIVHAAAPT 221
           S+ DE   +P  GAYS +TDL+ GF + +L+ AF  P+ +IE + N  +PPLIV     T
Sbjct: 165 SNNDECLHDPCFGAYSSITDLMFGFDETILQSAFGVPEGIIELMRNRTKPPLIVSETLCT 224

Query: 222 PSIKAKSSSPWEFEARLLKSFLGGDASAIEFNKKKKKKG---------IYNVYEVDPDFE 281
           P +    ++ W+ + RLLK F  G A  ++  KKK+KK           +NV+E +PDFE
Sbjct: 225 PGV----ANTWQLQPRLLKLF-AGSADLVDNKKKKEKKEKKEKVKKAKTFNVFESEPDFE 284

Query: 282 NCNGWSLTVTKKNSHQLKGSNIGFLVVNLTAGSMMGPHWNPRAWEIGIVTSDEPGVIRVG 341
           +  G ++T+ +K+   LKGS +G  +VNLT GSMMGPHWNP A EI IV     G++RV 
Sbjct: 285 SPYGRTITINRKDLKVLKGSMVGVSMVNLTQGSMMGPHWNPWACEISIVLKG-AGMVRVL 344

Query: 342 CSSTSAN-SSKCKNWSFVVEKGDVFVVPRFHPMAQMSFNNGTFVFVGFSTTNGHNMPQFF 401
            SS S+N SS+CKN  F VE+GD+F VPR HPMAQMSFNN + VFVGF+T+  +N PQF 
Sbjct: 345 RSSISSNTSSECKNVRFKVEEGDIFAVPRLHPMAQMSFNNDSLVFVGFTTSAKNNEPQFL 404

Query: 402 AGSSSVLKIVDREVLAWSFDVNVTTIDRLLKARVESIVLECTSCAEEEVRKM-------- 458
           AG  S L+++DR+VLA S +V+  TID LL A+ E+++LEC SCAE E+ K+        
Sbjct: 405 AGEDSALRMLDRQVLAASLNVSSVTIDGLLGAQKEAVILECHSCAEGEIEKLKVEIERKK 464

BLAST of Cucsa.104990 vs. Swiss-Prot
Match: VCL22_ARATH (Vicilin-like seed storage protein At2g28490 OS=Arabidopsis thaliana GN=At2g28490 PE=2 SV=1)

HSP 1 Score: 120.6 bits (301), Expect = 4.6e-26
Identity = 74/217 (34.10%), Postives = 119/217 (54.84%), Query Frame = 1

Query: 223 SSPWEFEARLLKSFLGGDASAIEFNKKKKKKGIYNVYEVD--PDFENCNGWSLTVTKKNS 282
           SS W +   +++S L       + +   + +  YN+Y+    P F+N  GWS+ +   + 
Sbjct: 297 SSGWSWR-NIVRSILDLTEEKNKGSGSSECEDSYNIYDKKDKPSFDNKYGWSIALDYDDY 356

Query: 283 HQLKGSNIGFLVVNLTAGSMMGPHWNPRAWEIGIVTSDEPGVIRVGCSSTSANSSKCKNW 342
             LK S IG  +VNLTAG+MM PH NP A E GIV +    +  V  + TSA +++    
Sbjct: 357 KPLKHSGIGVYLVNLTAGAMMAPHMNPTATEYGIVLAGSGEIQVVFPNGTSAMNTR---- 416

Query: 343 SFVVEKGDVFVVPRFHPMAQMSFNNGTFVFVGFSTTNGHNMPQFFAGSSSVLKIVDREVL 402
              V  GDVF +PR+    Q++   G F FVGF+T+   N PQF  GS+S+L+ ++   L
Sbjct: 417 ---VSVGDVFWIPRYFAFCQIASRTGPFEFVGFTTSAHKNRPQFLVGSNSLLRTLNLTSL 476

Query: 403 AWSFDVNVTTIDRLLKARVESIVLECTSCAEEEVRKM 438
           + +F V+  T+ R ++A+ E+++L   + A   V +M
Sbjct: 477 SIAFGVDEETMRRFIEAQREAVILPTPAAAPPHVGEM 505

HSP 2 Score: 62.4 bits (150), Expect = 1.5e-08
Identity = 57/224 (25.45%), Postives = 98/224 (43.75%), Query Frame = 1

Query: 54  ILKTEYGEISAVDFDDGTRFGR-YHLQFITLEPNSLFLPVLLHSDMVFYVHTGSGRLNWF 113
           ++K+E GE+  V    G    +  H+ F+T+EP +LF+P  L S ++ ++  G   L   
Sbjct: 94  VIKSEGGEMRVVLSPRGRIIEKPMHIGFLTMEPKTLFVPQYLDSSLLIFIRQGEATLGVI 153

Query: 114 DDNDLKEVDLRRGDLYRLHPGSIFYLQSSLETEREKLRIYALFSSTDEDSFNPSIGAY-- 173
             ++  E  L+ GD+Y +  GS+FYL ++   +R  L +      T    F      Y  
Sbjct: 154 CKDEFGERKLKAGDIYWIPAGSVFYLHNTGLGQR--LHVICSIDPTQSLGFETFQPFYIG 213

Query: 174 SRVTDLVRGFGKEVLRKAF-MAPDEVIEEIMNAKRPPLIVHAAAPTPSIKAKSSSPWEFE 233
              + ++ GF    L  AF ++  E+ + +M+  R P++     P P      S+ W   
Sbjct: 214 GGPSSVLAGFDPHTLTSAFNVSLPELQQMMMSQFRGPIVYVTEGPQPQ---PQSTVW--- 273

Query: 234 ARLLKSFLGGDASAIEFNKKKKKKGIYNVYEVDP-DFENCNGWS 273
                 FLG         K K+ K +    +  P D +  +GWS
Sbjct: 274 ----TQFLGLRGE----EKHKQLKKLLETKQGSPQDQQYSSGWS 301

BLAST of Cucsa.104990 vs. Swiss-Prot
Match: AMP22_MACIN (Vicilin-like antimicrobial peptides 2-2 OS=Macadamia integrifolia GN=AMP2-2 PE=2 SV=1)

HSP 1 Score: 100.9 bits (250), Expect = 3.8e-20
Identity = 95/389 (24.42%), Postives = 161/389 (41.39%), Query Frame = 1

Query: 56  KTEYGEISAVDFDDGTR-----FGRYHLQFITLEPNSLFLPVLLHSDMVFYVHTGSGRLN 115
           +TE G IS ++   G          Y L  +   PN+  LP  L +D +  V  G G L 
Sbjct: 264 RTEEGHISVLENFYGRSKLLRALKNYRLVLLEANPNAFVLPTHLDADAILLVTGGRGALK 323

Query: 116 WFDDNDLKEVDLRRGDLYRLHPGSIFYLQSSLETEREKLRIYALFSSTDED--SFNPSIG 175
               ++ +  +L  GD+ R+  G+ FYL +    ER  +  +    ST      F P+ G
Sbjct: 324 MIHRDNRESYNLECGDVIRIPAGTTFYLINRDNNERLHIAKFLQTISTPGQYKEFFPAGG 383

Query: 176 AYSRVTDLVRGFGKEVLRKAFMAPDEVIEEIMNAKRPPLIVHAAAPTPSIKAKSSSPWEF 235
                   +  F KE+L  A     E +  ++  +R  +I+ A+        +  S    
Sbjct: 384 --QNPEPYLSTFSKEILEAALNTQAERLRGVLGQQREGVIISASQEQIRELTRDDS---- 443

Query: 236 EARLLKSFLGGDASAIEFNKKKKKKGIYNVYEVDPDFENCNGWSLTVTKKNSHQLKGSNI 295
           E+R      GG++S          +G YN++   P + N  G +  V  ++  QL+  ++
Sbjct: 444 ESRRWHIRRGGESS----------RGPYNLFNKRPLYSNKYGQAYEVKPEDYRQLQDMDV 503

Query: 296 GFLVVNLTAGSMMGPHWNPRAWEIGIVTSDEPGVIRVGCSSTSANSSKCKNWSFVVEKGD 355
              + N+T GSMMGP +N R+ ++ +V S E  V  + C   S      +      E+ D
Sbjct: 504 SVFIANITQGSMMGPFFNTRSTKVVVVASGEADV-EMACPHLSGRHGGRRGGKRHEEEED 563

Query: 356 V--------------FVVPRFHPMAQMSFNNGTFVFVGFSTTNGHNMPQFFAG-SSSVLK 415
           V               VVP  HP+  +S  N   +   F     +N   F AG   +VL+
Sbjct: 564 VHYEQVKARLSKREAIVVPVGHPVVFVSSGNENLLLFAFGINAQNNHENFLAGRERNVLQ 623

Query: 416 IVDREVLAWSFDVNVTTIDRLLKARVESI 423
            ++ + +  +F      ++ L  ++ ESI
Sbjct: 624 QIEPQAMELAFAAPRKEVEELFNSQDESI 635

BLAST of Cucsa.104990 vs. Swiss-Prot
Match: AMP23_MACIN (Vicilin-like antimicrobial peptides 2-3 (Fragment) OS=Macadamia integrifolia GN=AMP2-3 PE=1 SV=1)

HSP 1 Score: 97.1 bits (240), Expect = 5.5e-19
Identity = 92/389 (23.65%), Postives = 160/389 (41.13%), Query Frame = 1

Query: 56  KTEYGEISAVDFDDGTR-----FGRYHLQFITLEPNSLFLPVLLHSDMVFYVHTGSGRLN 115
           +TE G IS ++   G          Y L  +   PN+  LP  L +D +  V  G G L 
Sbjct: 223 RTEEGHISVLENFYGRSKLLRALKNYRLVLLEANPNAFVLPTHLDADAILLVIGGRGALK 282

Query: 116 WFDDNDLKEVDLRRGDLYRLHPGSIFYLQSSLETEREKLRIYALFSSTDED--SFNPSIG 175
               ++ +  +L  GD+ R+  G+ FYL +    ER  +  +    ST      F P+ G
Sbjct: 283 MIHRDNRESYNLECGDVIRIPAGTTFYLINRDNNERLHIAKFLQTISTPGQYKEFFPAGG 342

Query: 176 AYSRVTDLVRGFGKEVLRKAFMAPDEVIEEIMNAKRPPLIVHAAAPTPSIKAKSSSPWEF 235
                   +  F KE+L  A     E +  ++  +R  +I+ A+        +  S    
Sbjct: 343 --QNPEPYLSTFSKEILEAALNTQTERLRGVLGQQREGVIIRASQEQIRELTRDDS---- 402

Query: 236 EARLLKSFLGGDASAIEFNKKKKKKGIYNVYEVDPDFENCNGWSLTVTKKNSHQLKGSNI 295
           E+R      GG++S          +G YN++   P + N  G +  V  ++  QL+  ++
Sbjct: 403 ESRRWHIRRGGESS----------RGPYNLFNKRPLYSNKYGQAYEVKPEDYRQLQDMDV 462

Query: 296 GFLVVNLTAGSMMGPHWNPRAWEIGIVTSDEPGVIRVGCSSTSANSS------------- 355
              + N+T GSMMGP +N R+ ++ +V S E  V  + C   S                 
Sbjct: 463 SVFIANITQGSMMGPFFNTRSTKVVVVASGEADV-EMACPHLSGRHGGRGGGKRHEEEEE 522

Query: 356 -KCKNWSFVVEKGDVFVVPRFHPMAQMSFNNGTFVFVGFSTTNGHNMPQFFAG-SSSVLK 415
              +     + K +  VV   HP+  +S  N   +   F     +N   F AG   +VL+
Sbjct: 523 VHYEQVRARLSKREAIVVLAGHPVVFVSSGNENLLLFAFGINAQNNHENFLAGRERNVLQ 582

Query: 416 IVDREVLAWSFDVNVTTIDRLLKARVESI 423
            ++ + +  +F  +   ++ L  ++ ESI
Sbjct: 583 QIEPQAMELAFAASRKEVEELFNSQDESI 594

BLAST of Cucsa.104990 vs. TrEMBL
Match: A0A0A0KGF2_CUCSA (PreproMP73 OS=Cucumis sativus GN=Csa_6G502040 PE=4 SV=1)

HSP 1 Score: 925.6 bits (2391), Expect = 2.3e-266
Identity = 457/457 (100.00%), Postives = 457/457 (100.00%), Query Frame = 1

Query: 1   MKKHSAVSVSGSSFSPSILIPIFFLFLSLPAYADDGWWEGDTPVVKRANERIPILKTEYG 60
           MKKHSAVSVSGSSFSPSILIPIFFLFLSLPAYADDGWWEGDTPVVKRANERIPILKTEYG
Sbjct: 1   MKKHSAVSVSGSSFSPSILIPIFFLFLSLPAYADDGWWEGDTPVVKRANERIPILKTEYG 60

Query: 61  EISAVDFDDGTRFGRYHLQFITLEPNSLFLPVLLHSDMVFYVHTGSGRLNWFDDNDLKEV 120
           EISAVDFDDGTRFGRYHLQFITLEPNSLFLPVLLHSDMVFYVHTGSGRLNWFDDNDLKEV
Sbjct: 61  EISAVDFDDGTRFGRYHLQFITLEPNSLFLPVLLHSDMVFYVHTGSGRLNWFDDNDLKEV 120

Query: 121 DLRRGDLYRLHPGSIFYLQSSLETEREKLRIYALFSSTDEDSFNPSIGAYSRVTDLVRGF 180
           DLRRGDLYRLHPGSIFYLQSSLETEREKLRIYALFSSTDEDSFNPSIGAYSRVTDLVRGF
Sbjct: 121 DLRRGDLYRLHPGSIFYLQSSLETEREKLRIYALFSSTDEDSFNPSIGAYSRVTDLVRGF 180

Query: 181 GKEVLRKAFMAPDEVIEEIMNAKRPPLIVHAAAPTPSIKAKSSSPWEFEARLLKSFLGGD 240
           GKEVLRKAFMAPDEVIEEIMNAKRPPLIVHAAAPTPSIKAKSSSPWEFEARLLKSFLGGD
Sbjct: 181 GKEVLRKAFMAPDEVIEEIMNAKRPPLIVHAAAPTPSIKAKSSSPWEFEARLLKSFLGGD 240

Query: 241 ASAIEFNKKKKKKGIYNVYEVDPDFENCNGWSLTVTKKNSHQLKGSNIGFLVVNLTAGSM 300
           ASAIEFNKKKKKKGIYNVYEVDPDFENCNGWSLTVTKKNSHQLKGSNIGFLVVNLTAGSM
Sbjct: 241 ASAIEFNKKKKKKGIYNVYEVDPDFENCNGWSLTVTKKNSHQLKGSNIGFLVVNLTAGSM 300

Query: 301 MGPHWNPRAWEIGIVTSDEPGVIRVGCSSTSANSSKCKNWSFVVEKGDVFVVPRFHPMAQ 360
           MGPHWNPRAWEIGIVTSDEPGVIRVGCSSTSANSSKCKNWSFVVEKGDVFVVPRFHPMAQ
Sbjct: 301 MGPHWNPRAWEIGIVTSDEPGVIRVGCSSTSANSSKCKNWSFVVEKGDVFVVPRFHPMAQ 360

Query: 361 MSFNNGTFVFVGFSTTNGHNMPQFFAGSSSVLKIVDREVLAWSFDVNVTTIDRLLKARVE 420
           MSFNNGTFVFVGFSTTNGHNMPQFFAGSSSVLKIVDREVLAWSFDVNVTTIDRLLKARVE
Sbjct: 361 MSFNNGTFVFVGFSTTNGHNMPQFFAGSSSVLKIVDREVLAWSFDVNVTTIDRLLKARVE 420

Query: 421 SIVLECTSCAEEEVRKMEEEAEREREEEEERKREEEE 458
           SIVLECTSCAEEEVRKMEEEAEREREEEEERKREEEE
Sbjct: 421 SIVLECTSCAEEEVRKMEEEAEREREEEEERKREEEE 457

BLAST of Cucsa.104990 vs. TrEMBL
Match: Q8W3X8_CUCMA (PreproMP73 OS=Cucurbita maxima GN=CmMP73 PE=2 SV=1)

HSP 1 Score: 709.1 bits (1829), Expect = 3.4e-201
Identity = 355/462 (76.84%), Postives = 397/462 (85.93%), Query Frame = 1

Query: 1   MKKHSAVSVSGSSFSPSILIPIFFLFLSLPAYADDGWWEGDTPVVKRANERIPILKTEYG 60
           MKK +A+S  GS FS S L  +FFLFLSLP+ ADD WWE   PV KRANER  +LKTEYG
Sbjct: 1   MKKCTAIS--GSPFSLSFLFTVFFLFLSLPSNADDKWWEAACPV-KRANERKSLLKTEYG 60

Query: 61  EISAVDFDDGTRFGRYHLQFITLEPNSLFLPVLLHSDMVFYVHTGSGRLNWFDDNDLKEV 120
           EISAVD  D ++FG YHLQFIT+EPNSLFLPVLLH+DMV Y+HTGSGRLNWFDD+DL+EV
Sbjct: 61  EISAVDLHDASQFGPYHLQFITMEPNSLFLPVLLHADMVLYMHTGSGRLNWFDDDDLREV 120

Query: 121 DLRRGDLYRLHPGSIFYLQSSLETEREKLRIYALFSSTDEDSFNPSIGAYSRVTDLVRGF 180
           DLRRGD++RL PG+IFY+ SSLETEREKLR+YALFSSTDED F P+IGAYSRVTD VRGF
Sbjct: 121 DLRRGDIFRLQPGAIFYIHSSLETEREKLRMYALFSSTDEDPFEPAIGAYSRVTDHVRGF 180

Query: 181 GKEVLRKAFMAPDEVIEEIMNAKRPPLIVHAAAPTPSIKAKS----SSPWEFEARLLKSF 240
            KE+L KAFM P+EVIEEIM+AKRPPLIVHAA    ++  K     S   E EAR LKSF
Sbjct: 181 DKEILCKAFMVPEEVIEEIMDAKRPPLIVHAATTLSTLSKKQRSSLSMSLELEARFLKSF 240

Query: 241 LGGDASAIEF-NKKKKKKGIYNVYEVDPDFENCNGWSLTVTKKNSHQLKGSNIGFLVVNL 300
           +GG    ++F  KKKKKKG+YNV+E DPDFENCNGWSLTVTKK SHQLKGSNIGF VVNL
Sbjct: 241 IGGGGIGMDFKKKKKKKKGLYNVFEADPDFENCNGWSLTVTKKVSHQLKGSNIGFFVVNL 300

Query: 301 TAGSMMGPHWNPRAWEIGIVTSDEPGVIRVGCSSTSANSSKCKNWSFVVEKGDVFVVPRF 360
           TAGSMMGPHWNPRAWEIGIVTS+E GV+RVGCSS + NSSKCK WSFVV KGDVFVVPRF
Sbjct: 301 TAGSMMGPHWNPRAWEIGIVTSEEAGVVRVGCSSMT-NSSKCKKWSFVVGKGDVFVVPRF 360

Query: 361 HPMAQMSFNNGTFVFVGFSTTNGHNMPQFFAGSSSVLKIVDREVLAWSFDVNVTTIDRLL 420
           HPMAQMSFNNG+F FVGFSTTN +N+PQF AG SSVL+ V+R+VLAWSFDVNVTTIDRLL
Sbjct: 361 HPMAQMSFNNGSFAFVGFSTTNRNNLPQFLAGRSSVLQTVERQVLAWSFDVNVTTIDRLL 420

Query: 421 KARVESIVLECTSCAEEEVRKMEEEAEREREEEEERKREEEE 458
           +ARVES++LECTSCAEEEV KMEEEAERER+EEEER+REEEE
Sbjct: 421 EARVESVILECTSCAEEEVMKMEEEAERERQEEEERRREEEE 458

BLAST of Cucsa.104990 vs. TrEMBL
Match: F6GWY5_VITVI (Putative uncharacterized protein OS=Vitis vinifera GN=VIT_04s0023g01390 PE=4 SV=1)

HSP 1 Score: 475.7 bits (1223), Expect = 6.3e-131
Identity = 254/462 (54.98%), Postives = 325/462 (70.35%), Query Frame = 1

Query: 15  SPSILIPIFFLFLSLPAYADDGWWEGDTPVVKRANERIPILKTEYGEISAVDFDDGTRFG 74
           S S+ + + +  L    ++ +G W G  P+  + ++R  I+ TEYGEISA    DGTR G
Sbjct: 9   SLSLFLFLAYFCLHAKGWSGNGDW-GAGPLFPK-DKRETIVSTEYGEISAAQVSDGTRRG 68

Query: 75  RYHLQFITLEPNSLFLPVLLHSDMVFYVHTGSGRLNWFDDNDLKEV--DLRRGDLYRLHP 134
            YHLQF TLEPNSLFLPVLLH+DMVFYVHTGSG+LNW ++   K     LRRGD+YRL P
Sbjct: 69  SYHLQFFTLEPNSLFLPVLLHTDMVFYVHTGSGKLNWANEEKEKTTLTALRRGDVYRLKP 128

Query: 135 GSIFYLQSSLETEREKLRIYALFSSTDEDSFNP--SIGAYSRVTDLVRGFGKEVLRKAFM 194
           G++FYLQS+LE+EREKLRIYA+F + ++   N   SIGAYS ++DLVRGF K+VL+ AF 
Sbjct: 129 GTVFYLQSNLESEREKLRIYAIFVNLEDGDLNEVSSIGAYSSISDLVRGFDKKVLQAAFE 188

Query: 195 APDEVIEEIMNAKRPPLIVHAAAPTPSIKAKSSSPWEFEARLLKSFLGGDASAI-EFNKK 254
             +E+IE I NA +PP IVH      ++ A+S + W +EAR LK+F+G    +I +   K
Sbjct: 189 VSEELIEAITNATKPPAIVH------NVPARSENLWGWEARFLKAFIGSQGHSIYDLENK 248

Query: 255 KKKKGIYNVYEVDPDFENCNGWSLTVTKKNSHQLKGSNIGFLVVNLTAGSMMGPHWNPRA 314
           KK    +N+ + DPDFENCNG +LTVT K+   LKGSNIG  +VNLT GSMMGPHWNP A
Sbjct: 249 KKAAKTFNIRDADPDFENCNGRALTVTTKDMKVLKGSNIGIFMVNLTKGSMMGPHWNPLA 308

Query: 315 WEIGIVTSDEPGVIRVGCSSTSANSS---------------KCKNWSFVVEKGDVFVVPR 374
            EI +V   + G++RV CSS +  SS               KC+N SF V +GDVFVVPR
Sbjct: 309 TEIAVVLEGQ-GIVRVVCSSNTTKSSSSNTTKSSSSNSTKFKCENRSFRVREGDVFVVPR 368

Query: 375 FHPMAQMSFNNGTFVFVGFSTTNGHNMPQFFAGSSSVLKIVDREVLAWSFDVNVTTIDRL 434
           FHPMAQMSFNNG+ VF+GFST +  N PQF AG SSVL+ +DR+VLA +F+V+ TT+D+ 
Sbjct: 369 FHPMAQMSFNNGSLVFMGFSTASKLNHPQFLAGESSVLRTLDRDVLAAAFNVSNTTMDQF 428

Query: 435 LKARVESIVLECTSCAEEEVRKMEEEAEREREEEEERKREEE 457
           L  + ESI+L+CTSCAEEE R MEEE E++R+EEE RK EEE
Sbjct: 429 LTPQRESIILDCTSCAEEEARMMEEEIEKKRQEEEARKEEEE 461

BLAST of Cucsa.104990 vs. TrEMBL
Match: F6GWY5_VITVI (Putative uncharacterized protein OS=Vitis vinifera GN=VIT_04s0023g01390 PE=4 SV=1)

HSP 1 Score: 28.5 bits (62), Expect = 2.7e+04
Identity = 18/32 (56.25%), Postives = 21/32 (65.62%), Query Frame = 1

Query: 432 EEVRKMEEEAE---REREE---EEERKREEEE 458
           E+   M EE E   REREE   E+E+KREEEE
Sbjct: 522 EQEEAMREEDERQKREREEEKYEKEKKREEEE 553

HSP 2 Score: 472.2 bits (1214), Expect = 7.0e-130
Identity = 239/434 (55.07%), Postives = 318/434 (73.27%), Query Frame = 1

Query: 25  LFLSLPAYADD-GWWEGDTPVVKRANERIPILKTEYGEISAVDFDDGTRFGRYHLQFITL 84
           L + + A+++D   WE   P + R   R  ++ TEYGEISA +   GT+ G YH+QFITL
Sbjct: 19  LSIHVEAFSEDVSAWE--RPYLVRRGHRRSLVVTEYGEISAAEISSGTK-GPYHIQFITL 78

Query: 85  EPNSLFLPVLLHSDMVFYVHTGSGRLNWFDDNDLKEVDLRRGDLYRLHPGSIFYLQSSLE 144
           EPNSL LPVLLH+DMVFYVHTG+G+L+W D  ++K ++LRRGD+YRL  GS+F+++S+L+
Sbjct: 79  EPNSLLLPVLLHADMVFYVHTGNGKLSWTDGREMKRMNLRRGDVYRLQAGSVFFVRSNLD 138

Query: 145 TEREKLRIYALFSSTDEDSFNPSIGAYSRVTDLVRGFGKEVLRKAFMAPDEVIEEIMNAK 204
           +ER+K+RI+A+FS+TDED + PSIGAYS V+DLV GF ++VL++AF  P+EV+EE+ +A 
Sbjct: 139 SERQKMRIHAIFSNTDEDIYEPSIGAYSSVSDLVLGFDRKVLQEAFKVPEEVLEELTSAT 198

Query: 205 RPPLIVHAAAPTPSIKAKSSSPWEFEARLLKSFLGGDASAIEFNKKKKKKGIYNVYEVDP 264
           +PP +VHA       K + S  WE E R+L   +G        +KK K+   +N+ +  P
Sbjct: 199 KPPAVVHAVT-----KDQKSVNWELEDRMLDFLIGNK------HKKTKETKTFNILDAKP 258

Query: 265 DFENCNGWSLTVTKKNSHQLKGSNIGFLVVNLTAGSMMGPHWNPRAWEIGIVTSDEPGVI 324
           DFENCNGWSLTV K +   L  SNIG  +VNLT GSMMGPHWNP A EI IV     G++
Sbjct: 259 DFENCNGWSLTVDKHSLKSLSDSNIGIFMVNLTKGSMMGPHWNPMATEIAIVLHGR-GMV 318

Query: 325 RVGCSSTSANSSKCKNWSFVVEKGDVFVVPRFHPMAQMSFNNGTFVFVGFSTTNGHNMPQ 384
           RV C ST AN S+CKN  F V++GDVF VPRFHPMAQ+SFNN +FVF+GFST+   N PQ
Sbjct: 319 RVICHST-ANESECKNMRFKVKEGDVFAVPRFHPMAQISFNNDSFVFMGFSTSTKRNHPQ 378

Query: 385 FFAGSSSVLKIVDREVLAWSFDVNVTTIDRLLKARVESIVLECTSCAEEEVRKMEEEAER 444
           F  G SS+L+I+DR +LA SF+V  TT+D+LL A+ E+++L+CTSCAE E  KM+EE E+
Sbjct: 379 FLTGKSSILQILDRGILAVSFNVTNTTMDQLLNAQEEALILDCTSCAEIEENKMKEEFEK 436

Query: 445 EREEEEERKREEEE 458
           E++EEE RKREEEE
Sbjct: 439 EKQEEEARKREEEE 436

BLAST of Cucsa.104990 vs. TrEMBL
Match: A0A0D2PCQ1_GOSRA (Uncharacterized protein OS=Gossypium raimondii GN=B456_007G205600 PE=4 SV=1)

HSP 1 Score: 469.9 bits (1208), Expect = 3.5e-129
Identity = 250/460 (54.35%), Postives = 329/460 (71.52%), Query Frame = 1

Query: 9   VSGSSFSPSILIPIFFLFLSLPAYAD-DGWWEGDTPVVKRA---NERIPILKTEYGEISA 68
           + G+  SP     +F + L+ P +   + +       V +    ++R  + +TE+GEISA
Sbjct: 4   LKGTLVSPFSFFAVFSIILASPTFLSVESYSHRHASTVGQLVLKHDRKLLTQTEHGEISA 63

Query: 69  VDFDDGTRFGRYHLQFITLEPNSLFLPVLLHSDMVFYVHTGSGRLNWFDDNDLKEVDLRR 128
               DGT+ G YHLQFITLEPNS+FLPVLLH++MVF+VHTGSG L+W DD+D+++V+LR+
Sbjct: 64  AIVTDGTK-GPYHLQFITLEPNSVFLPVLLHANMVFFVHTGSGTLSWTDDDDMRDVNLRK 123

Query: 129 GDLYRLHPGSIFYLQSSLETEREKLRIYALFSSTDEDSFNPSIGAYSRVTDLVRGFGKEV 188
           GD+YRL  GS FY+QSSLE EREKLRIYA+F+++++D + PSIGAYS +TD+V GF   V
Sbjct: 124 GDVYRLPEGSAFYVQSSLEAEREKLRIYAIFTNSEDDIYEPSIGAYSSITDVVLGFDTRV 183

Query: 189 LRKAFMAPDEVIEEIMNAKRPPLIVHAAAPTPSIKAKSSSPWEFEARLLKSFLGGDAS-- 248
           L+ AF  P+++I EIM A +PP I+HA      ++ +     E++ARLLK+FLGG+    
Sbjct: 184 LQAAFKVPEDLI-EIMRASKPPAIIHA------VQKEGKMLVEWQARLLKAFLGGNRDGF 243

Query: 249 --AIEFNKKKKKKGIYNVYEVDPDFENCNGWSLTVTKKNSHQLKGSNIGFLVVNLTAGSM 308
             +I   K KKK   YNV E DPDFENCNGWSLTV +K+ H LK SNIG  +VNLT GSM
Sbjct: 244 FHSINGKKTKKKMRAYNVLEADPDFENCNGWSLTVDRKDLHILKHSNIGLFMVNLTKGSM 303

Query: 309 MGPHWNPRAWEIGIVTSDEPGVIRVGCSSTSANSSKCKNWSFVVEKGDVFVVPRFHPMAQ 368
           M PHWNPRA EI IV   + G++RV CSS S    +C++  + V +GD+F VPRFHPMAQ
Sbjct: 304 MAPHWNPRASEIAIVLQGQ-GMVRVVCSSNS-KECECRDMRYRVREGDIFAVPRFHPMAQ 363

Query: 369 MSFNNGTFVFVGFST-TNGHNMPQFFAGSSSVLKIVDREVLAWSFDVNVTTIDRLLKARV 428
           MSFNN +FVF+GFST T   N PQF  G  S L+I+D+++LA SF+V+  TID+LLK + 
Sbjct: 364 MSFNNDSFVFMGFSTLTTKRNHPQFLVGKKSALRIMDKQILALSFNVSNATIDQLLKPQR 423

Query: 429 ESIVLECTSCAEEEVRKMEEEAEREREEEEE--RKREEEE 458
           ES++L+CTSCAEEE R M EE ERER+EEEE  R+REEEE
Sbjct: 424 ESVILDCTSCAEEEERLMVEEIERERKEEEEEARRREEEE 453

BLAST of Cucsa.104990 vs. TAIR10
Match: AT2G18540.1 (AT2G18540.1 RmlC-like cupins superfamily protein)

HSP 1 Score: 381.3 bits (978), Expect = 8.2e-106
Identity = 205/426 (48.12%), Postives = 286/426 (67.14%), Query Frame = 1

Query: 42  TPVVKRANERIPILKTEYGEISAVDFDDGTRFGRYHLQFITLEPNSLFLPVLLHSDMVFY 101
           +P++ + ++R  ++ TE+G ISAV   DG     YH+QFITLEPN+L LP+LLHSDMVF+
Sbjct: 41  SPLLVKKDQRTSVVATEFGNISAVQIGDG-----YHIQFITLEPNALLLPLLLHSDMVFF 100

Query: 102 VHTGSGRLNWFDDNDLKEVDLRRGDLYRLHPGSIFYLQSSLETEREKLRIYALFSSTDED 161
           VHTG+G LNW D+   ++++LRRGD++RL  G++FY+ S+     EKLR+YA+F+   + 
Sbjct: 101 VHTGTGILNWIDEESERKLELRRGDVFRLRSGTVFYVHSN-----EKLRVYAIFN-VGKC 160

Query: 162 SFNPSIGAYSRVTDLVRGFGKEVLRKAFMAPDEVIEEIMNAKRPPLIVHAAAPTPSIKAK 221
             +P +GAYS V DL+ GF    LR AF  P++++ +I +A +PPLIV+A    P  + +
Sbjct: 161 LNDPCLGAYSSVRDLLLGFDDRTLRSAFAVPEDILRKIRDATKPPLIVNAL---PRNRTQ 220

Query: 222 SSSPWEFEARLLKSFLGGDASAIEFNKK------KKKKGIYNVYEVDPDFENCNGWSLTV 281
                ++++RL++ F+  +        K      KKK   +NV+E DPDFEN NG S+ V
Sbjct: 221 GLEEDKWQSRLVRLFVSVEDVTDHLAMKPIVDTNKKKSRTFNVFEEDPDFENNNGRSIVV 280

Query: 282 TKKNSHQLKGSNIGFLVVNLTAGSMMGPHWNPRAWEIGIVTSDEPGVIRVGCSSTSANSS 341
            +K+   LKGS  G  +VNLT GSM+GPHWNP A EI IV   E  V  V   S S+  +
Sbjct: 281 DEKDLDALKGSRFGVFMVNLTKGSMIGPHWNPSACEISIVLEGEGMVRVVNQQSLSSCKN 340

Query: 342 KCKNWSFVVEKGDVFVVPRFHPMAQMSFNNGTFVFVGFSTTNGHNMPQFFAGSSSVLKIV 401
             K+ SF+VE+GDVFVVP+FHPMAQMSF N +FVF+GFST+   N PQF  G SSVLK++
Sbjct: 341 DRKSESFMVEEGDVFVVPKFHPMAQMSFENSSFVFMGFSTSAKTNHPQFLVGQSSVLKVL 400

Query: 402 DREVLAWSFDVNVTTIDRLLKARVESIVLECTSCAEEE----VRKMEEEAEREREEEEER 458
           DR+V+A SF+++  TI  LLKA+ ES++ EC SCAE E    +R++EE   RE EE E R
Sbjct: 401 DRDVVAVSFNLSNETIKGLLKAQKESVIFECASCAEGELSKLMREIEERKRREEEEIERR 452

HSP 2 Score: 37.7 bits (86), Expect = 2.2e-02
Identity = 17/26 (65.38%), Postives = 22/26 (84.62%), Query Frame = 1

Query: 431 EEEVRKMEEEAEREREEEEERKREEE 457
           EEE +K EEEAE+ R+ EEER++EEE
Sbjct: 503 EEERKKREEEAEQARKREEEREKEEE 528

HSP 3 Score: 35.4 bits (80), Expect = 1.1e-01
Identity = 19/27 (70.37%), Postives = 21/27 (77.78%), Query Frame = 1

Query: 431 EEEVRKMEEEAER-EREEEEERKREEE 457
           EE  R+ EEE ER +REEEE RKREEE
Sbjct: 465 EEAKRREEEETERKKREEEEARKREEE 491

HSP 4 Score: 35.4 bits (80), Expect = 1.1e-01
Identity = 19/26 (73.08%), Postives = 20/26 (76.92%), Query Frame = 1

Query: 432 EEV-RKMEEEAEREREEEEERKREEE 457
           EEV RK  EE ER+R EEE RKREEE
Sbjct: 543 EEVERKRREEQERKRREEEARKREEE 568

HSP 5 Score: 34.7 bits (78), Expect = 1.9e-01
Identity = 16/26 (61.54%), Postives = 18/26 (69.23%), Query Frame = 1

Query: 432 EEVRKMEEEAEREREEEEERKREEEE 458
           EE RK EEEA +  EEE  +K EEEE
Sbjct: 668 EEERKREEEAAKRAEEERRKKEEEEE 693

HSP 6 Score: 32.0 bits (71), Expect = 1.2e+00
Identity = 16/26 (61.54%), Postives = 21/26 (80.77%), Query Frame = 1

Query: 432 EEV-RKMEEEAEREREEEEERKREEE 457
           EEV RK+ EE ER+REEE  ++RE+E
Sbjct: 589 EEVERKIREEQERKREEEMAKRREQE 614

HSP 7 Score: 30.8 bits (68), Expect = 2.7e+00
Identity = 16/26 (61.54%), Postives = 20/26 (76.92%), Query Frame = 1

Query: 431 EEEVRKMEEEAEREREEEEERKREEE 457
           EEE ++ EEEA  +R EEE +KREEE
Sbjct: 489 EEERKREEEEA--KRREEERKKREEE 512

HSP 8 Score: 30.8 bits (68), Expect = 2.7e+00
Identity = 19/42 (45.24%), Postives = 21/42 (50.00%), Query Frame = 1

Query: 431 EEE---------------VRKMEEEAEREREEEEERKREEEE 458
           EEE                RK  EE ER+R EE+ERKR EEE
Sbjct: 520 EEEREKEEEMAKKREEERQRKEREEVERKRREEQERKRREEE 561

HSP 9 Score: 30.0 bits (66), Expect = 4.6e+00
Identity = 15/26 (57.69%), Postives = 18/26 (69.23%), Query Frame = 1

Query: 431 EEEVRKMEEEAEREREEEEERKREEE 457
           EEE RK EEE  + RE+E +RK  EE
Sbjct: 566 EEE-RKREEEMAKRREQERQRKEREE 590

HSP 10 Score: 28.9 bits (63), Expect = 1.0e+01
Identity = 16/32 (50.00%), Postives = 20/32 (62.50%), Query Frame = 1

Query: 431 EEEVRKMEEEAEREREE-----EEERKREEEE 458
           E E +K EEEA +  EE     EEER+R+E E
Sbjct: 622 EMERKKREEEARKREEEMAKIREEERQRKERE 653

HSP 11 Score: 28.5 bits (62), Expect = 1.3e+01
Identity = 15/29 (51.72%), Postives = 19/29 (65.52%), Query Frame = 1

Query: 430 AE--EEVRKMEEEAEREREEEEERKREEE 457
           A   EE R+ EEE  ++REEE +RK  EE
Sbjct: 516 ARKREEEREKEEEMAKKREEERQRKEREE 544

HSP 12 Score: 27.7 bits (60), Expect = 2.3e+01
Identity = 13/26 (50.00%), Postives = 18/26 (69.23%), Query Frame = 1

Query: 431 EEEVRKMEEEAEREREEEEERKREEE 457
           E+E RK EEE  + RE+E ++K  EE
Sbjct: 598 EQE-RKREEEMAKRREQERQKKEREE 622

HSP 13 Score: 27.7 bits (60), Expect = 2.3e+01
Identity = 20/35 (57.14%), Postives = 22/35 (62.86%), Query Frame = 1

Query: 431 EEEVRKM-EE-----EAEREREEEE--ERKREEEE 458
           EEE RK  EE     E ER+R+E E  ERKR EEE
Sbjct: 629 EEEARKREEEMAKIREEERQRKEREDVERKRREEE 663

BLAST of Cucsa.104990 vs. TAIR10
Match: AT4G36700.1 (AT4G36700.1 RmlC-like cupins superfamily protein)

HSP 1 Score: 380.2 bits (975), Expect = 1.8e-105
Identity = 214/453 (47.24%), Postives = 294/453 (64.90%), Query Frame = 1

Query: 42  TPVVKRANERIPILKTEYGEISAVDFDDGTR-FGRYHLQFITLEPNSLFLPVLLHSDMVF 101
           +P++ + ++  PI +T++G+IS V   +G    G Y +  ITLEPN++ LP+LLHSDMVF
Sbjct: 45  SPLLIKKDQWKPIFETKFGQISTVQIGNGCGGMGPYKIHSITLEPNTILLPLLLHSDMVF 104

Query: 102 YVHTGSGRLNWFDDNDLKEVDLRRGDLYRLHPGSIFYLQSS-----LETEREKLRIYALF 161
           +V +GSG LNW D+ + K  ++R GD+YRL PGS+FYLQS      L T   KL++YA+F
Sbjct: 105 FVDSGSGILNWVDE-EAKSTEIRLGDVYRLRPGSVFYLQSKPVDIFLGT---KLKLYAIF 164

Query: 162 SSTDEDSFNPSIGAYSRVTDLVRGFGKEVLRKAFMAPDEVIEEIMNAKRPPLIVHAAAPT 221
           S+ DE   +P  GAYS +TDL+ GF + +L+ AF  P+ +IE + N  +PPLIV     T
Sbjct: 165 SNNDECLHDPCFGAYSSITDLMFGFDETILQSAFGVPEGIIELMRNRTKPPLIVSETLCT 224

Query: 222 PSIKAKSSSPWEFEARLLKSFLGGDASAIEFNKKKKKKG---------IYNVYEVDPDFE 281
           P +    ++ W+ + RLLK F  G A  ++  KKK+KK           +NV+E +PDFE
Sbjct: 225 PGV----ANTWQLQPRLLKLF-AGSADLVDNKKKKEKKEKKEKVKKAKTFNVFESEPDFE 284

Query: 282 NCNGWSLTVTKKNSHQLKGSNIGFLVVNLTAGSMMGPHWNPRAWEIGIVTSDEPGVIRVG 341
           +  G ++T+ +K+   LKGS +G  +VNLT GSMMGPHWNP A EI IV     G++RV 
Sbjct: 285 SPYGRTITINRKDLKVLKGSMVGVSMVNLTQGSMMGPHWNPWACEISIVLKG-AGMVRVL 344

Query: 342 CSSTSAN-SSKCKNWSFVVEKGDVFVVPRFHPMAQMSFNNGTFVFVGFSTTNGHNMPQFF 401
            SS S+N SS+CKN  F VE+GD+F VPR HPMAQMSFNN + VFVGF+T+  +N PQF 
Sbjct: 345 RSSISSNTSSECKNVRFKVEEGDIFAVPRLHPMAQMSFNNDSLVFVGFTTSAKNNEPQFL 404

Query: 402 AGSSSVLKIVDREVLAWSFDVNVTTIDRLLKARVESIVLECTSCAEEEVRKM-------- 458
           AG  S L+++DR+VLA S +V+  TID LL A+ E+++LEC SCAE E+ K+        
Sbjct: 405 AGEDSALRMLDRQVLAASLNVSSVTIDGLLGAQKEAVILECHSCAEGEIEKLKVEIERKK 464

BLAST of Cucsa.104990 vs. TAIR10
Match: AT2G28490.1 (AT2G28490.1 RmlC-like cupins superfamily protein)

HSP 1 Score: 120.6 bits (301), Expect = 2.6e-27
Identity = 74/217 (34.10%), Postives = 119/217 (54.84%), Query Frame = 1

Query: 223 SSPWEFEARLLKSFLGGDASAIEFNKKKKKKGIYNVYEVD--PDFENCNGWSLTVTKKNS 282
           SS W +   +++S L       + +   + +  YN+Y+    P F+N  GWS+ +   + 
Sbjct: 297 SSGWSWR-NIVRSILDLTEEKNKGSGSSECEDSYNIYDKKDKPSFDNKYGWSIALDYDDY 356

Query: 283 HQLKGSNIGFLVVNLTAGSMMGPHWNPRAWEIGIVTSDEPGVIRVGCSSTSANSSKCKNW 342
             LK S IG  +VNLTAG+MM PH NP A E GIV +    +  V  + TSA +++    
Sbjct: 357 KPLKHSGIGVYLVNLTAGAMMAPHMNPTATEYGIVLAGSGEIQVVFPNGTSAMNTR---- 416

Query: 343 SFVVEKGDVFVVPRFHPMAQMSFNNGTFVFVGFSTTNGHNMPQFFAGSSSVLKIVDREVL 402
              V  GDVF +PR+    Q++   G F FVGF+T+   N PQF  GS+S+L+ ++   L
Sbjct: 417 ---VSVGDVFWIPRYFAFCQIASRTGPFEFVGFTTSAHKNRPQFLVGSNSLLRTLNLTSL 476

Query: 403 AWSFDVNVTTIDRLLKARVESIVLECTSCAEEEVRKM 438
           + +F V+  T+ R ++A+ E+++L   + A   V +M
Sbjct: 477 SIAFGVDEETMRRFIEAQREAVILPTPAAAPPHVGEM 505

HSP 2 Score: 62.4 bits (150), Expect = 8.4e-10
Identity = 57/224 (25.45%), Postives = 98/224 (43.75%), Query Frame = 1

Query: 54  ILKTEYGEISAVDFDDGTRFGR-YHLQFITLEPNSLFLPVLLHSDMVFYVHTGSGRLNWF 113
           ++K+E GE+  V    G    +  H+ F+T+EP +LF+P  L S ++ ++  G   L   
Sbjct: 94  VIKSEGGEMRVVLSPRGRIIEKPMHIGFLTMEPKTLFVPQYLDSSLLIFIRQGEATLGVI 153

Query: 114 DDNDLKEVDLRRGDLYRLHPGSIFYLQSSLETEREKLRIYALFSSTDEDSFNPSIGAY-- 173
             ++  E  L+ GD+Y +  GS+FYL ++   +R  L +      T    F      Y  
Sbjct: 154 CKDEFGERKLKAGDIYWIPAGSVFYLHNTGLGQR--LHVICSIDPTQSLGFETFQPFYIG 213

Query: 174 SRVTDLVRGFGKEVLRKAF-MAPDEVIEEIMNAKRPPLIVHAAAPTPSIKAKSSSPWEFE 233
              + ++ GF    L  AF ++  E+ + +M+  R P++     P P      S+ W   
Sbjct: 214 GGPSSVLAGFDPHTLTSAFNVSLPELQQMMMSQFRGPIVYVTEGPQPQ---PQSTVW--- 273

Query: 234 ARLLKSFLGGDASAIEFNKKKKKKGIYNVYEVDP-DFENCNGWS 273
                 FLG         K K+ K +    +  P D +  +GWS
Sbjct: 274 ----TQFLGLRGE----EKHKQLKKLLETKQGSPQDQQYSSGWS 301

BLAST of Cucsa.104990 vs. NCBI nr
Match: gi|778722460|ref|XP_011658490.1| (PREDICTED: LOW QUALITY PROTEIN: provicilin [Cucumis sativus])

HSP 1 Score: 925.6 bits (2391), Expect = 3.3e-266
Identity = 457/457 (100.00%), Postives = 457/457 (100.00%), Query Frame = 1

Query: 1   MKKHSAVSVSGSSFSPSILIPIFFLFLSLPAYADDGWWEGDTPVVKRANERIPILKTEYG 60
           MKKHSAVSVSGSSFSPSILIPIFFLFLSLPAYADDGWWEGDTPVVKRANERIPILKTEYG
Sbjct: 1   MKKHSAVSVSGSSFSPSILIPIFFLFLSLPAYADDGWWEGDTPVVKRANERIPILKTEYG 60

Query: 61  EISAVDFDDGTRFGRYHLQFITLEPNSLFLPVLLHSDMVFYVHTGSGRLNWFDDNDLKEV 120
           EISAVDFDDGTRFGRYHLQFITLEPNSLFLPVLLHSDMVFYVHTGSGRLNWFDDNDLKEV
Sbjct: 61  EISAVDFDDGTRFGRYHLQFITLEPNSLFLPVLLHSDMVFYVHTGSGRLNWFDDNDLKEV 120

Query: 121 DLRRGDLYRLHPGSIFYLQSSLETEREKLRIYALFSSTDEDSFNPSIGAYSRVTDLVRGF 180
           DLRRGDLYRLHPGSIFYLQSSLETEREKLRIYALFSSTDEDSFNPSIGAYSRVTDLVRGF
Sbjct: 121 DLRRGDLYRLHPGSIFYLQSSLETEREKLRIYALFSSTDEDSFNPSIGAYSRVTDLVRGF 180

Query: 181 GKEVLRKAFMAPDEVIEEIMNAKRPPLIVHAAAPTPSIKAKSSSPWEFEARLLKSFLGGD 240
           GKEVLRKAFMAPDEVIEEIMNAKRPPLIVHAAAPTPSIKAKSSSPWEFEARLLKSFLGGD
Sbjct: 181 GKEVLRKAFMAPDEVIEEIMNAKRPPLIVHAAAPTPSIKAKSSSPWEFEARLLKSFLGGD 240

Query: 241 ASAIEFNKKKKKKGIYNVYEVDPDFENCNGWSLTVTKKNSHQLKGSNIGFLVVNLTAGSM 300
           ASAIEFNKKKKKKGIYNVYEVDPDFENCNGWSLTVTKKNSHQLKGSNIGFLVVNLTAGSM
Sbjct: 241 ASAIEFNKKKKKKGIYNVYEVDPDFENCNGWSLTVTKKNSHQLKGSNIGFLVVNLTAGSM 300

Query: 301 MGPHWNPRAWEIGIVTSDEPGVIRVGCSSTSANSSKCKNWSFVVEKGDVFVVPRFHPMAQ 360
           MGPHWNPRAWEIGIVTSDEPGVIRVGCSSTSANSSKCKNWSFVVEKGDVFVVPRFHPMAQ
Sbjct: 301 MGPHWNPRAWEIGIVTSDEPGVIRVGCSSTSANSSKCKNWSFVVEKGDVFVVPRFHPMAQ 360

Query: 361 MSFNNGTFVFVGFSTTNGHNMPQFFAGSSSVLKIVDREVLAWSFDVNVTTIDRLLKARVE 420
           MSFNNGTFVFVGFSTTNGHNMPQFFAGSSSVLKIVDREVLAWSFDVNVTTIDRLLKARVE
Sbjct: 361 MSFNNGTFVFVGFSTTNGHNMPQFFAGSSSVLKIVDREVLAWSFDVNVTTIDRLLKARVE 420

Query: 421 SIVLECTSCAEEEVRKMEEEAEREREEEEERKREEEE 458
           SIVLECTSCAEEEVRKMEEEAEREREEEEERKREEEE
Sbjct: 421 SIVLECTSCAEEEVRKMEEEAEREREEEEERKREEEE 457

BLAST of Cucsa.104990 vs. NCBI nr
Match: gi|700193611|gb|KGN48815.1| (PreproMP73 [Cucumis sativus])

HSP 1 Score: 925.6 bits (2391), Expect = 3.3e-266
Identity = 457/457 (100.00%), Postives = 457/457 (100.00%), Query Frame = 1

Query: 1   MKKHSAVSVSGSSFSPSILIPIFFLFLSLPAYADDGWWEGDTPVVKRANERIPILKTEYG 60
           MKKHSAVSVSGSSFSPSILIPIFFLFLSLPAYADDGWWEGDTPVVKRANERIPILKTEYG
Sbjct: 1   MKKHSAVSVSGSSFSPSILIPIFFLFLSLPAYADDGWWEGDTPVVKRANERIPILKTEYG 60

Query: 61  EISAVDFDDGTRFGRYHLQFITLEPNSLFLPVLLHSDMVFYVHTGSGRLNWFDDNDLKEV 120
           EISAVDFDDGTRFGRYHLQFITLEPNSLFLPVLLHSDMVFYVHTGSGRLNWFDDNDLKEV
Sbjct: 61  EISAVDFDDGTRFGRYHLQFITLEPNSLFLPVLLHSDMVFYVHTGSGRLNWFDDNDLKEV 120

Query: 121 DLRRGDLYRLHPGSIFYLQSSLETEREKLRIYALFSSTDEDSFNPSIGAYSRVTDLVRGF 180
           DLRRGDLYRLHPGSIFYLQSSLETEREKLRIYALFSSTDEDSFNPSIGAYSRVTDLVRGF
Sbjct: 121 DLRRGDLYRLHPGSIFYLQSSLETEREKLRIYALFSSTDEDSFNPSIGAYSRVTDLVRGF 180

Query: 181 GKEVLRKAFMAPDEVIEEIMNAKRPPLIVHAAAPTPSIKAKSSSPWEFEARLLKSFLGGD 240
           GKEVLRKAFMAPDEVIEEIMNAKRPPLIVHAAAPTPSIKAKSSSPWEFEARLLKSFLGGD
Sbjct: 181 GKEVLRKAFMAPDEVIEEIMNAKRPPLIVHAAAPTPSIKAKSSSPWEFEARLLKSFLGGD 240

Query: 241 ASAIEFNKKKKKKGIYNVYEVDPDFENCNGWSLTVTKKNSHQLKGSNIGFLVVNLTAGSM 300
           ASAIEFNKKKKKKGIYNVYEVDPDFENCNGWSLTVTKKNSHQLKGSNIGFLVVNLTAGSM
Sbjct: 241 ASAIEFNKKKKKKGIYNVYEVDPDFENCNGWSLTVTKKNSHQLKGSNIGFLVVNLTAGSM 300

Query: 301 MGPHWNPRAWEIGIVTSDEPGVIRVGCSSTSANSSKCKNWSFVVEKGDVFVVPRFHPMAQ 360
           MGPHWNPRAWEIGIVTSDEPGVIRVGCSSTSANSSKCKNWSFVVEKGDVFVVPRFHPMAQ
Sbjct: 301 MGPHWNPRAWEIGIVTSDEPGVIRVGCSSTSANSSKCKNWSFVVEKGDVFVVPRFHPMAQ 360

Query: 361 MSFNNGTFVFVGFSTTNGHNMPQFFAGSSSVLKIVDREVLAWSFDVNVTTIDRLLKARVE 420
           MSFNNGTFVFVGFSTTNGHNMPQFFAGSSSVLKIVDREVLAWSFDVNVTTIDRLLKARVE
Sbjct: 361 MSFNNGTFVFVGFSTTNGHNMPQFFAGSSSVLKIVDREVLAWSFDVNVTTIDRLLKARVE 420

Query: 421 SIVLECTSCAEEEVRKMEEEAEREREEEEERKREEEE 458
           SIVLECTSCAEEEVRKMEEEAEREREEEEERKREEEE
Sbjct: 421 SIVLECTSCAEEEVRKMEEEAEREREEEEERKREEEE 457

BLAST of Cucsa.104990 vs. NCBI nr
Match: gi|659080244|ref|XP_008440688.1| (PREDICTED: globulin-1 S allele [Cucumis melo])

HSP 1 Score: 874.4 bits (2258), Expect = 8.7e-251
Identity = 427/458 (93.23%), Postives = 444/458 (96.94%), Query Frame = 1

Query: 1   MKKHSAVSVSGSSFSPSILIPIFFLFLSLPAYADDGWWEGDTPVVKRANERIPILKTEYG 60
           MKK +A+SVSGS FS S LI IFFLF SLPAYADDGWWEGD+PVVKRANERI +LKTEYG
Sbjct: 1   MKKPTAISVSGSPFSLSFLISIFFLFFSLPAYADDGWWEGDSPVVKRANERIQLLKTEYG 60

Query: 61  EISAVDFDDGTRFGRYHLQFITLEPNSLFLPVLLHSDMVFYVHTGSGRLNWFDDNDLKEV 120
           +ISAVDFDDG+RFG YHLQFITLEPNSLFLPVLLHSDMVFY+HTGSGRLNWFD+NDLKEV
Sbjct: 61  DISAVDFDDGSRFGPYHLQFITLEPNSLFLPVLLHSDMVFYIHTGSGRLNWFDENDLKEV 120

Query: 121 DLRRGDLYRLHPGSIFYLQSSLETEREKLRIYALFSSTDEDSFNPSIGAYSRVTDLVRGF 180
           DLRRGDLYRLHPGSIFYLQSSLE EREKLRIYALFSSTDEDSFNPS+GAYSRVTDLVRGF
Sbjct: 121 DLRRGDLYRLHPGSIFYLQSSLEIEREKLRIYALFSSTDEDSFNPSLGAYSRVTDLVRGF 180

Query: 181 GKEVLRKAFMAPDEVIEEIMNAKRPPLIVHAAAPTPSIKAKSSSPWEFEARLLKSFLGGD 240
           GKEVLRKAFMAPDEVIEEIM AKRPPLIVHAAAPTPSI+AKSSSPWEFEARLLK+FLGGD
Sbjct: 181 GKEVLRKAFMAPDEVIEEIMTAKRPPLIVHAAAPTPSIRAKSSSPWEFEARLLKAFLGGD 240

Query: 241 ASAIEFN-KKKKKKGIYNVYEVDPDFENCNGWSLTVTKKNSHQLKGSNIGFLVVNLTAGS 300
           AS IEFN KKKKKKGIYNVYEVDPDFENCNGWSLTVTKKNSHQLKGSNIGFLVVNLTAGS
Sbjct: 241 ASGIEFNKKKKKKKGIYNVYEVDPDFENCNGWSLTVTKKNSHQLKGSNIGFLVVNLTAGS 300

Query: 301 MMGPHWNPRAWEIGIVTSDEPGVIRVGCSSTSANSSKCKNWSFVVEKGDVFVVPRFHPMA 360
           MMGPHWNPRAWEIGIVTSDEPGV+ VGCSSTSANSSKCKNWSFVVEKGD+FVVPRFHPMA
Sbjct: 301 MMGPHWNPRAWEIGIVTSDEPGVVHVGCSSTSANSSKCKNWSFVVEKGDIFVVPRFHPMA 360

Query: 361 QMSFNNGTFVFVGFSTTNGHNMPQFFAGSSSVLKIVDREVLAWSFDVNVTTIDRLLKARV 420
           QMSFNNGTFVFVGFSTTNGHNMPQFF GSSSVL++VDREVLAWSFDVNVTT+DRLLKARV
Sbjct: 361 QMSFNNGTFVFVGFSTTNGHNMPQFFVGSSSVLQLVDREVLAWSFDVNVTTVDRLLKARV 420

Query: 421 ESIVLECTSCAEEEVRKMEEEAEREREEEEERKREEEE 458
           ESI+LECTSCAEEEVRKMEEEAEREREEEEERKREEEE
Sbjct: 421 ESIILECTSCAEEEVRKMEEEAEREREEEEERKREEEE 458

BLAST of Cucsa.104990 vs. NCBI nr
Match: gi|17221648|dbj|BAB78478.1| (preproMP73 [Cucurbita maxima])

HSP 1 Score: 709.1 bits (1829), Expect = 4.9e-201
Identity = 355/462 (76.84%), Postives = 397/462 (85.93%), Query Frame = 1

Query: 1   MKKHSAVSVSGSSFSPSILIPIFFLFLSLPAYADDGWWEGDTPVVKRANERIPILKTEYG 60
           MKK +A+S  GS FS S L  +FFLFLSLP+ ADD WWE   PV KRANER  +LKTEYG
Sbjct: 1   MKKCTAIS--GSPFSLSFLFTVFFLFLSLPSNADDKWWEAACPV-KRANERKSLLKTEYG 60

Query: 61  EISAVDFDDGTRFGRYHLQFITLEPNSLFLPVLLHSDMVFYVHTGSGRLNWFDDNDLKEV 120
           EISAVD  D ++FG YHLQFIT+EPNSLFLPVLLH+DMV Y+HTGSGRLNWFDD+DL+EV
Sbjct: 61  EISAVDLHDASQFGPYHLQFITMEPNSLFLPVLLHADMVLYMHTGSGRLNWFDDDDLREV 120

Query: 121 DLRRGDLYRLHPGSIFYLQSSLETEREKLRIYALFSSTDEDSFNPSIGAYSRVTDLVRGF 180
           DLRRGD++RL PG+IFY+ SSLETEREKLR+YALFSSTDED F P+IGAYSRVTD VRGF
Sbjct: 121 DLRRGDIFRLQPGAIFYIHSSLETEREKLRMYALFSSTDEDPFEPAIGAYSRVTDHVRGF 180

Query: 181 GKEVLRKAFMAPDEVIEEIMNAKRPPLIVHAAAPTPSIKAKS----SSPWEFEARLLKSF 240
            KE+L KAFM P+EVIEEIM+AKRPPLIVHAA    ++  K     S   E EAR LKSF
Sbjct: 181 DKEILCKAFMVPEEVIEEIMDAKRPPLIVHAATTLSTLSKKQRSSLSMSLELEARFLKSF 240

Query: 241 LGGDASAIEF-NKKKKKKGIYNVYEVDPDFENCNGWSLTVTKKNSHQLKGSNIGFLVVNL 300
           +GG    ++F  KKKKKKG+YNV+E DPDFENCNGWSLTVTKK SHQLKGSNIGF VVNL
Sbjct: 241 IGGGGIGMDFKKKKKKKKGLYNVFEADPDFENCNGWSLTVTKKVSHQLKGSNIGFFVVNL 300

Query: 301 TAGSMMGPHWNPRAWEIGIVTSDEPGVIRVGCSSTSANSSKCKNWSFVVEKGDVFVVPRF 360
           TAGSMMGPHWNPRAWEIGIVTS+E GV+RVGCSS + NSSKCK WSFVV KGDVFVVPRF
Sbjct: 301 TAGSMMGPHWNPRAWEIGIVTSEEAGVVRVGCSSMT-NSSKCKKWSFVVGKGDVFVVPRF 360

Query: 361 HPMAQMSFNNGTFVFVGFSTTNGHNMPQFFAGSSSVLKIVDREVLAWSFDVNVTTIDRLL 420
           HPMAQMSFNNG+F FVGFSTTN +N+PQF AG SSVL+ V+R+VLAWSFDVNVTTIDRLL
Sbjct: 361 HPMAQMSFNNGSFAFVGFSTTNRNNLPQFLAGRSSVLQTVERQVLAWSFDVNVTTIDRLL 420

Query: 421 KARVESIVLECTSCAEEEVRKMEEEAEREREEEEERKREEEE 458
           +ARVES++LECTSCAEEEV KMEEEAERER+EEEER+REEEE
Sbjct: 421 EARVESVILECTSCAEEEVMKMEEEAERERQEEEERRREEEE 458

BLAST of Cucsa.104990 vs. NCBI nr
Match: gi|1009145108|ref|XP_015890153.1| (PREDICTED: vicilin-like seed storage protein At2g18540 [Ziziphus jujuba])

HSP 1 Score: 510.0 bits (1312), Expect = 4.3e-141
Identity = 264/445 (59.33%), Postives = 325/445 (73.03%), Query Frame = 1

Query: 18  ILIPIFFLFLS-----LPAYADDGWWEGDTPVVKRANERIPILKTEYGEISAVDFDDGTR 77
           I + IF  F+S     L A  +D  + G    V + ++R  ++ TEYGEIS ++  DG +
Sbjct: 10  ISVAIFICFISINLFSLSAALNDEEFSGHVGPVVKKDQRKSLVVTEYGEISVIEIADGIK 69

Query: 78  FGRYHLQFITLEPNSLFLPVLLHSDMVFYVHTGSGRLNWFDDNDLKEVDLRRGDLYRLHP 137
            G +H+Q ITLEPNSLFLPVLLH+DMVFYVHTGSGRL+W  D DLK VDL+RGD++RL P
Sbjct: 70  -GPHHIQLITLEPNSLFLPVLLHADMVFYVHTGSGRLSWVKDEDLKTVDLKRGDIFRLQP 129

Query: 138 GSIFYLQSSLETEREKLRIYALFSSTDEDSFNPSIGAYSRVTDLVRGFGKEVLRKAFMAP 197
           GS+FY++S LETEREKLRIYA+F ++D++  + SIG YS + DLVRGFGK  L+ AF  P
Sbjct: 130 GSVFYIESDLETEREKLRIYAIFVNSDDEDLHTSIGVYSSINDLVRGFGKRTLQAAFKVP 189

Query: 198 DEVIEEIMNAKRPPLIVHAAAPTPSIKAKSSSPWEFEARLLKSFLGGDASAIEFNKKKKK 257
           +EVI+ I NA    LIVHA       K +     E  AR LK+ +G    +IE  KKKKK
Sbjct: 190 EEVIQSITNATERRLIVHAGP-----KEEKEDTSELGARYLKALVG----SIENKKKKKK 249

Query: 258 KGIYNVYEVDPDFENCNGWSLTVTKKNSHQLKGSNIGFLVVNLTAGSMMGPHWNPRAWEI 317
              YN+    PDFENCNGWS TVTKK+S  LKGSNIG  +VNLT GSMMGPHWNP+A EI
Sbjct: 250 DKAYNIRHAKPDFENCNGWSTTVTKKDSDLLKGSNIGIFMVNLTKGSMMGPHWNPKASEI 309

Query: 318 GIVTSDEPGVIRVGCSSTSANSSKCKNWSFVVEKGDVFVVPRFHPMAQMSFNNGTFVFVG 377
            IV   + G++RVGC S+S NS+KCKN  F V++GDVF V RFHPMAQ+SFNN +FVF+G
Sbjct: 310 SIVLHGQ-GMVRVGCPSSSTNSTKCKNMRFKVKEGDVFCVTRFHPMAQISFNNDSFVFMG 369

Query: 378 FSTTNGHNMPQFFAGSSSVLKIVDREVLAWSFDVNVTTIDRLLKARVESIVLECTSCAEE 437
           FSTT G N PQF AG SSVL+ +DR+VLA SF+V  TTIDRLL  +VES++L+CTSCAEE
Sbjct: 370 FSTTTGRNHPQFLAGKSSVLRTLDRDVLAVSFNVTNTTIDRLLAPQVESVILDCTSCAEE 429

Query: 438 EVRKMEEEAEREREEEEERKREEEE 458
           E R MEEE E+E+EEEE R+REEEE
Sbjct: 430 EERFMEEEIEKEKEEEEAREREEEE 443