Cucsa.104830 (gene) Cucumber (Gy14) v1

NameCucsa.104830
Typegene
OrganismCucumis sativus (Cucumber (Gy14) v1)
DescriptionPentatricopeptide repeat-containing protein, putative
Locationscaffold00927 : 3173011 .. 3175122 (-)
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
CTATCAACTCTCACTTGGTGGAACTCCAGCATACGAGGCGCTGTAAATCAAGGCAATGCGAGCAAAGCTCTCGCTCTTTTCCATCAATTGAAGTTAAATGGTCTACAACCAAACAATTTTACATTTCCCTTCTTATCAAAAGCTTGCGCCAAGCTCTCCCATCTTACTAACTCCCAAATCATCCATACCCATGTTGTCAAATCCCCATTTTACTCTGATATCTATGTCCAGACGGCCATGGTTGATATGTATGTCAAGTGTGGTAAAGTGGATGATGCATATAACTTGTTTGATAAAATGCCTGTTAGGAATATTGCATCATGGAACGCTATGATTATTGGTTTTTCTCAGATCGGCTCACTTGATAGAGTGTTCAATCTGTTTATGGGGATGAGATTAGTGGGAACTCGGCCAGATGCAGCTACTGTCATTGGATTGACTCGAGCAGTTATATCTGCCAAAAGTTTAAGGTTTTTAAAAGCTGTTCATGCTATTGGGATTGAAACAGGATTAGATGCTGACACCTCAGTTTCTAACACTTGGATTGCTGCCTATTCCAAATGCGGTGAACTGCAGCTTGCAAAGATGGTGTTCCATGGGATTCAAAAGACTGCAAGATCTTCTGTCTCGTGGAATTCACTGATTGCATGCTATGCTCACTTTGGGAAATATGTAGATGCTGTAAAATCTTACAAAGGATTGCTCTGTGATGGTTTTAAGCCTGATGCAAGTACGATTATTAGTTTGCTTTCTTCTTGCCAGCAACCTGAAGCACTAATTTATGGTTTCCTTATTCATGGCCATGGTTTCCAATTAGGATGCGATTCAGATATTTCTTTAATTAACACCCTTATATCAATGTATTCAAGGTGTGGAGATATTAGCTCTGCTACAATTTTGTTTGATGGTATGTCTATTAGAACTTGTGTTTCATGGACGGCCATGATTAGTGGGTATAGTGAGGTGGGGCGAGTTGATGATGCCTTGGTATTATTTAATGCTATGGAAGAAACTGGAGAAAAACCTGATATAGTTACTGTTCTTTCCCTGATATCAGGTTGCGGTAAAACAGGGGCACTTGGGCTTGGACATTGGATTGACAACTATGCATCTTTACATGAATTGAAAAAAGATGTGGTAGTTTGTAATGCATTGATAGACATGTATGCAAAATGTGGGAGTTTGAATGATGCTCGAGAGATATTCTACAGTTTGCCTAATAGAACTGTTGTCTCTTGGACAGCCATGATTGCAGCTTGTGCTCTGAATGGGGAATTTAGAGAAGCTTTAGATCTCTTCTCACTGTTGTCAGAGTCAGGAATAGAGCCAAATAATATCACGTTTCTAGCTGTTCTTCAAGCTTGCTGTCATGGAGGCTATCTCGAGAAAGGAAGGGAATGTTTTATGATGATGACTGAAAGATATGGTATAAATCCTGGTTTGGATCATTATTCTTGCATGATCGATCTTCTTGGACGGAAAGGGAAGCTAATTGAAGCATTGGAGGTTATTCAAGATATGCCTATGAAGCCTGACGAGGGCATATGGGGTGCATTGCTTGGTGCTTGTAAGATTCACAACAACATGGAAATCGGTGAGTATGTGTNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNCTCGTTATCTCTTTGAATTACAGCCTCGGGTTGCAGTTTCGTTCGTCGAGATGGCAAATATATATGCATCAGTTGGAAGATGGGATGAAGTTGCAGCTATGAGAAAAACGATGAGATCAAACCAAATGAGGAAATCCCCTGGGAAAAGCGTTGTTCAAGTGAATGGAATGTCTCACGTGTTTTTTGTTGAGGACAGAAGCCATCACGACAGTTTGCTTATTTATGAAGCGTTGGGAAATTTAGCAATGCAGATGAAACAAAAAGAATTCTCGTCACATGCTCAGAGGTGGGTTGAGCTTGATACGATATGA

mRNA sequence

CTATCAACTCTCACTTGGTGGAACTCCAGCATACGAGGCGCTGTAAATCAAGGCAATGCGAGCAAAGCTCTCGCTCTTTTCCATCAATTGAAGTTAAATGGTCTACAACCAAACAATTTTACATTTCCCTTCTTATCAAAAGCTTGCGCCAAGCTCTCCCATCTTACTAACTCCCAAATCATCCATACCCATGTTGTCAAATCCCCATTTTACTCTGATATCTATGTCCAGACGGCCATGGTTGATATGTATGTCAAGTGTGGTAAAGTGGATGATGCATATAACTTGTTTGATAAAATGCCTGTTAGGAATATTGCATCATGGAACGCTATGATTATTGGTTTTTCTCAGATCGGCTCACTTGATAGAGTGTTCAATCTGTTTATGGGGATGAGATTAGTGGGAACTCGGCCAGATGCAGCTACTGTCATTGGATTGACTCGAGCAGTTATATCTGCCAAAAGTTTAAGGTTTTTAAAAGCTGTTCATGCTATTGGGATTGAAACAGGATTAGATGCTGACACCTCAGTTTCTAACACTTGGATTGCTGCCTATTCCAAATGCGGTGAACTGCAGCTTGCAAAGATGGTGTTCCATGGGATTCAAAAGACTGCAAGATCTTCTGTCTCGTGGAATTCACTGATTGCATGCTATGCTCACTTTGGGAAATATGTAGATGCTGTAAAATCTTACAAAGGATTGCTCTGTGATGGTTTTAAGCCTGATGCAAGATGCGATTCAGATATTTCTTTAATTAACACCCTTATATCAATGTATTCAAGTGGGTATAGTGAGGTGGGGCGAGTTGATGATGCCTTGGTATTATTTAATGCTATGGAAGAAACTGGAGAAAAACCTGATATAGTTACTGTTCTTTCCCTGATATCAGGTTGCGGTAAAACAGGGGCACTTGGGCTTGGACATTGGATTGACAACTATGCATCTTTACATGAATTGAAAAAAGATGTGGTAGTTTGTAATGCATTGATAGACATGTATGCAAAATGTGGGAGTTTGAATGATGCTCGAGAGATATTCTACAGTTTGCCTAATAGAACTGTTGTCTCTTGGACAGCCATGATTGCAGCTTGTGCTCTGAATGGGGAATTTAGAGAAGCTTTAGATCTCTTCTCACTGTTGTCAGAGTCAGGAATAGAGCCAAATAATATCACCCTGACGAGGGCATATGGGGTGCATTGCTTGGTGCTTGTAAGATTCACAACAACATGGAAATCGCCTCGGGTTGCAGTTTCGTTCGTCGAGATGGCAAATATATATGCATCAGTTGGAAGATGGGATGAAGTTGCAGCTATGAGAAAAACGATGAGATCAAACCAAATGAGGAAATCCCCTGGGAAAAGCGTTGTTCAAGTGAATGGAATGTCTCACGTGTTTTTTGTTGAGGACAGAAGCCATCACGACAGTTTGCTTATTTATGAAGCGTTGGGAAATTTAGCAATGCAGATGAAACAAAAAGAATTCTCGTCACATGCTCAGAGGTGGGTTGAGCTTGATACGATATGA

Coding sequence (CDS)

ATGGTTGATATGTATGTCAAGTGTGGTAAAGTGGATGATGCATATAACTTGTTTGATAAAATGCCTGTTAGGAATATTGCATCATGGAACGCTATGATTATTGGTTTTTCTCAGATCGGCTCACTTGATAGAGTGTTCAATCTGTTTATGGGGATGAGATTAGTGGGAACTCGGCCAGATGCAGCTACTGTCATTGGATTGACTCGAGCAGTTATATCTGCCAAAAGTTTAAGGTTTTTAAAAGCTGTTCATGCTATTGGGATTGAAACAGGATTAGATGCTGACACCTCAGTTTCTAACACTTGGATTGCTGCCTATTCCAAATGCGGTGAACTGCAGCTTGCAAAGATGGTGTTCCATGGGATTCAAAAGACTGCAAGATCTTCTGTCTCGTGGAATTCACTGATTGCATGCTATGCTCACTTTGGGAAATATGTAGATGCTGTAAAATCTTACAAAGGATTGCTCTGTGATGGTTTTAAGCCTGATGCAAGATGCGATTCAGATATTTCTTTAATTAACACCCTTATATCAATGTATTCAAGTGGGTATAGTGAGGTGGGGCGAGTTGATGATGCCTTGGTATTATTTAATGCTATGGAAGAAACTGGAGAAAAACCTGATATAGTTACTGTTCTTTCCCTGATATCAGGTTGCGGTAAAACAGGGGCACTTGGGCTTGGACATTGGATTGACAACTATGCATCTTTACATGAATTGAAAAAAGATGTGGTAGTTTGTAATGCATTGATAGACATGTATGCAAAATGTGGGAGTTTGAATGATGCTCGAGAGATATTCTACAGTTTGCCTAATAGAACTGTTGTCTCTTGGACAGCCATGATTGCAGCTTGTGCTCTGAATGGGGAATTTAGAGAAGCTTTAGATCTCTTCTCACTGTTGTCAGAGTCAGGAATAGAGCCAAATAATATCACCCTGACGAGGGCATATGGGGTGCATTGCTTGGTGCTTGTAAGATTCACAACAACATGGAAATCGCCTCGGGTTGCAGTTTCGTTCGTCGAGATGGCAAATATATATGCATCAGTTGGAAGATGGGATGAAGTTGCAGCTATGAGAAAAACGATGAGATCAAACCAAATGAGGAAATCCCCTGGGAAAAGCGTTGTTCAAGTGAATGGAATGTCTCACGTGTTTTTTGTTGAGGACAGAAGCCATCACGACAGTTTGCTTATTTATGAAGCGTTGGGAAATTTAGCAATGCAGATGAAACAAAAAGAATTCTCGTCACATGCTCAGAGGTGGGTTGAGCTTGATACGATATGA

Protein sequence

MVDMYVKCGKVDDAYNLFDKMPVRNIASWNAMIIGFSQIGSLDRVFNLFMGMRLVGTRPDAATVIGLTRAVISAKSLRFLKAVHAIGIETGLDADTSVSNTWIAAYSKCGELQLAKMVFHGIQKTARSSVSWNSLIACYAHFGKYVDAVKSYKGLLCDGFKPDARCDSDISLINTLISMYSSGYSEVGRVDDALVLFNAMEETGEKPDIVTVLSLISGCGKTGALGLGHWIDNYASLHELKKDVVVCNALIDMYAKCGSLNDAREIFYSLPNRTVVSWTAMIAACALNGEFREALDLFSLLSESGIEPNNITLTRAYGVHCLVLVRFTTTWKSPRVAVSFVEMANIYASVGRWDEVAAMRKTMRSNQMRKSPGKSVVQVNGMSHVFFVEDRSHHDSLLIYEALGNLAMQMKQKEFSSHAQRWVELDTI*
BLAST of Cucsa.104830 vs. Swiss-Prot
Match: PP323_ARATH (Pentatricopeptide repeat-containing protein At4g19191, mitochondrial OS=Arabidopsis thaliana GN=PCMP-E1 PE=2 SV=1)

HSP 1 Score: 302.8 bits (774), Expect = 6.2e-81
Identity = 167/368 (45.38%), Postives = 223/368 (60.60%), Query Frame = 1

Query: 2   VDMYVKCGKVDDAYNLFDKMPVRNIASWNAMIIGFSQIGSLDRVFNLFMGMRLVGTRPDA 61
           VDM+VKC  VD A  +F++MP R+  +WNAM+ GF Q G  D+ F+LF  MRL    PD+
Sbjct: 94  VDMFVKCNSVDYAAKVFERMPERDATTWNAMLSGFCQSGHTDKAFSLFREMRLNEITPDS 153

Query: 62  ATVIGLTRAVISAKSLRFLKAVHAIGIETGLDADTSVSNTWIAAYSKCGELQLAKMVFHG 121
            TV+ L ++    KSL+ L+A+HA+GI  G+D   +V+NTWI+ Y KCG+L  AK+VF  
Sbjct: 154 VTVMTLIQSASFEKSLKLLEAMHAVGIRLGVDVQVTVANTWISTYGKCGDLDSAKLVFEA 213

Query: 122 IQKTARSSVSWNSLIACYAHFGKYVDAVKSYKGLLCDGFKPD--------ARC------- 181
           I +  R+ VSWNS+   Y+ FG+  DA   Y  +L + FKPD        A C       
Sbjct: 214 IDRGDRTVVSWNSMFKAYSVFGEAFDAFGLYCLMLREEFKPDLSTFINLAASCQNPETLT 273

Query: 182 --------------DSDISLINTLISMYS---------------------------SGYS 241
                         D DI  INT ISMYS                           SGY+
Sbjct: 274 QGRLIHSHAIHLGTDQDIEAINTFISMYSKSEDTCSARLLFDIMTSRTCVSWTVMISGYA 333

Query: 242 EVGRVDDALVLFNAMEETGEKPDIVTVLSLISGCGKTGALGLGHWIDNYASLHELKKD-V 301
           E G +D+AL LF+AM ++GEKPD+VT+LSLISGCGK G+L  G WID  A ++  K+D V
Sbjct: 334 EKGDMDEALALFHAMIKSGEKPDLVTLLSLISGCGKFGSLETGKWIDARADIYGCKRDNV 393

Query: 302 VVCNALIDMYAKCGSLNDAREIFYSLPNRTVVSWTAMIAACALNGEFREALDLFSLLSES 313
           ++CNALIDMY+KCGS+++AR+IF + P +TVV+WT MIA  ALNG F EAL LFS + + 
Sbjct: 394 MICNALIDMYSKCGSIHEARDIFDNTPEKTVVTWTTMIAGYALNGIFLEALKLFSKMIDL 453


HSP 2 Score: 97.8 bits (242), Expect = 3.0e-19
Identity = 85/345 (24.64%), Postives = 154/345 (44.64%), Query Frame = 1

Query: 80  LKAVHAIGIETGLDADTSVSNTWIAAYSKCGELQLAKMV-----FHGIQKTARSSVSWNS 139
           L   HA+ I++G   D     + I+   K G L+  K +      +G ++   + +  N+
Sbjct: 342 LALFHAM-IKSGEKPDLVTLLSLISGCGKFGSLETGKWIDARADIYGCKRD--NVMICNA 401

Query: 140 LIACYAHFGKYVDAVKSYKGLLCDGFKPDARCDSDISLINTLISMYSSGYSEVGRVDDAL 199
           LI  Y+  G   +A   +          D   +  +    T+I+    GY+  G   +AL
Sbjct: 402 LIDMYSKCGSIHEARDIF----------DNTPEKTVVTWTTMIA----GYALNGIFLEAL 461

Query: 200 VLFNAMEETGEKPDIVTVLSLISGCGKTGALGLG-HWIDNYASLHELKKDVVVCNALIDM 259
            LF+ M +   KP+ +T L+++  C  +G+L  G  +      ++ +   +   + ++D+
Sbjct: 462 KLFSKMIDLDYKPNHITFLAVLQACAHSGSLEKGWEYFHIMKQVYNISPGLDHYSCMVDL 521

Query: 260 YAKCGSLNDAREIFYSLPNRTVVS-WTAMIAACALNGEFR----EALDLFSLLSESGIEP 319
             + G L +A E+  ++  +     W A++ AC ++   +     A  LF+L        
Sbjct: 522 LGRKGKLEEALELIRNMSAKPDAGIWGALLNACKIHRNVKIAEQAAESLFNL-------- 581

Query: 320 NNITLTRAYGVHCLVLVRFTTTWKSPRVAVSFVEMANIYASVGRWDEVAAMRKTMRSNQM 379
                                    P++A  +VEMANIYA+ G WD  A +R  M+   +
Sbjct: 582 ------------------------EPQMAAPYVEMANIYAAAGMWDGFARIRSIMKQRNI 637

Query: 380 RKSPGKSVVQVNGMSHVFFVEDRSHHDSLLIYEALGNLAMQMKQK 414
           +K PG+SV+QVNG +H F V +  H ++ +IY  L  L++  K K
Sbjct: 642 KKYPGESVIQVNGKNHSFTVGEHGHVENEVIYFTLNGLSLFAKDK 637


HSP 3 Score: 52.0 bits (123), Expect = 1.9e-05
Identity = 37/149 (24.83%), Postives = 69/149 (46.31%), Query Frame = 1

Query: 1   MVDMYVKCGKVDDAYNLFDKMPVRNIASWNAMIIGFSQIGSLDRVFNLFMGMRLVGTRPD 60
           ++DMY KCG + +A ++FD  P + + +W  MI G++  G       LF  M  +  +P+
Sbjct: 399 LIDMYSKCGSIHEARDIFDNTPEKTVVTWTTMIAGYALNGIFLEALKLFSKMIDLDYKPN 458

Query: 61  AATVIGLTRAVISAKSL-------RFLKAVHAIGIETGLDADTSVSNTWIAAYSKCGELQ 120
             T + + +A   + SL         +K V+   I  GLD  + +    +    + G+L+
Sbjct: 459 HITFLAVLQACAHSGSLEKGWEYFHIMKQVY--NISPGLDHYSCM----VDLLGRKGKLE 518

Query: 121 LAKMVFHGIQKTARSSVSWNSLI-ACYAH 142
            A  +   +     + + W +L+ AC  H
Sbjct: 519 EALELIRNMSAKPDAGI-WGALLNACKIH 540

BLAST of Cucsa.104830 vs. Swiss-Prot
Match: PP169_ARATH (Pentatricopeptide repeat-containing protein At2g22410, mitochondrial OS=Arabidopsis thaliana GN=PCMP-E28 PE=2 SV=1)

HSP 1 Score: 181.4 bits (459), Expect = 2.1e-44
Identity = 99/320 (30.94%), Postives = 170/320 (53.12%), Query Frame = 1

Query: 2   VDMYVKCGKVDDAYNLFDKMPVRNIASWNAMIIGFSQIGSLDRVFNLFMGMRLVGTRPDA 61
           + M+  CG +++A  +FD+ PVR++ SWN +I G+ +IG  ++   ++  M   G +PD 
Sbjct: 198 IHMFASCGDMENARKVFDESPVRDLVSWNCLINGYKKIGEAEKAIYVYKLMESEGVKPDD 257

Query: 62  ATVIGLTRAVISAKSLRFLKAVHAIGIETGLDADTSVSNTWIAAYSKCGELQLAKMVFHG 121
            T+IGL  +      L   K  +    E GL     + N  +  +SKCG++  A+ +F  
Sbjct: 258 VTMIGLVSSCSMLGDLNRGKEFYEYVKENGLRMTIPLVNALMDMFSKCGDIHEARRIFDN 317

Query: 122 IQKTARSSVSWNSLIACYAHFGKYVDAVKSYKGLLCDGFKPDARCDSDISLINTLISMYS 181
           ++K  R+ VSW ++I+ YA  G    + K +          D   + D+ L N +I    
Sbjct: 318 LEK--RTIVSWTTMISGYARCGLLDVSRKLF----------DDMEEKDVVLWNAMIG--- 377

Query: 182 SGYSEVGRVDDALVLFNAMEETGEKPDIVTVLSLISGCGKTGALGLGHWIDNYASLHELK 241
            G  +  R  DAL LF  M+ +  KPD +T++  +S C + GAL +G WI  Y   + L 
Sbjct: 378 -GSVQAKRGQDALALFQEMQTSNTKPDEITMIHCLSACSQLGALDVGIWIHRYIEKYSLS 437

Query: 242 KDVVVCNALIDMYAKCGSLNDAREIFYSLPNRTVVSWTAMIAACALNGEFREALDLFSLL 301
            +V +  +L+DMYAKCG++++A  +F+ +  R  +++TA+I   AL+G+   A+  F+ +
Sbjct: 438 LNVALGTSLVDMYAKCGNISEALSVFHGIQTRNSLTYTAIIGGLALHGDASTAISYFNEM 497

Query: 302 SESGIEPNNITLTRAYGVHC 322
            ++GI P+ IT        C
Sbjct: 498 IDAGIAPDEITFIGLLSACC 501


HSP 2 Score: 105.1 bits (261), Expect = 1.9e-21
Identity = 98/415 (23.61%), Postives = 164/415 (39.52%), Query Frame = 1

Query: 1   MVDMYVKCGKVDDAYNLFDKMPVRNIASWNAMIIGFSQIGSLDRVFNLFMGMRLVGTRPD 60
           ++DM+ KCG + +A  +FD +  R I SW  MI G+++ G LD    LF  M        
Sbjct: 298 LMDMFSKCGDIHEARRIFDNLEKRTIVSWTTMISGYARCGLLDVSRKLFDDMEEKDVVLW 357

Query: 61  AATVIGLTRAVISAKSLRFLKAVHAIGIETGLDADTSVSNTWIAAYSKCGELQLAKMVFH 120
            A + G  +A     +L   + +      +    D       ++A S+ G L +   +  
Sbjct: 358 NAMIGGSVQAKRGQDALALFQEMQT----SNTKPDEITMIHCLSACSQLGALDVGIWIHR 417

Query: 121 GIQKTARS--SVSWNSLIACYAHFGKYVDAVKSYKGLLCDGFKPDARCDSDISLINTLIS 180
            I+K + S       SL+  YA  G   +A+  + G+            +  SL  T I 
Sbjct: 418 YIEKYSLSLNVALGTSLVDMYAKCGNISEALSVFHGI-----------QTRNSLTYTAI- 477

Query: 181 MYSSGYSEVGRVDDALVLFNAMEETGEKPDIVTVLSLISGCGKTGALGLGH-WIDNYASL 240
               G +  G    A+  FN M + G  PD +T + L+S C   G +  G  +     S 
Sbjct: 478 --IGGLALHGDASTAISYFNEMIDAGIAPDEITFIGLLSACCHGGMIQTGRDYFSQMKSR 537

Query: 241 HELKKDVVVCNALIDMYAKCGSLNDAREIFYSLP-NRTVVSWTAMIAACALNGEFREALD 300
             L   +   + ++D+  + G L +A  +  S+P       W A++  C ++G       
Sbjct: 538 FNLNPQLKHYSIMVDLLGRAGLLEEADRLMESMPMEADAAVWGALLFGCRMHG------- 597

Query: 301 LFSLLSESGIEPNNITLTRAYGVHCLVLVRFTTTWKSPRVAVSFVEMANIYASVGRWDEV 360
                        N+ L        L L         P  +  +V +  +Y     W++ 
Sbjct: 598 -------------NVELGEKAAKKLLEL--------DPSDSGIYVLLDGMYGEANMWEDA 657

Query: 361 AAMRKTMRSNQMRKSPGKSVVQVNGMSHVFFVEDRSHHDSLLIYEALGNLAMQMK 412
              R+ M    + K PG S ++VNG+   F V D+S  +S  IY+ L  L   M+
Sbjct: 658 KRARRMMNERGVEKIPGCSSIEVNGIVCEFIVRDKSRPESEKIYDRLHCLGRHMR 666


HSP 3 Score: 102.4 bits (254), Expect = 1.2e-20
Identity = 89/304 (29.28%), Postives = 142/304 (46.71%), Query Frame = 1

Query: 25  NIASWNAMIIGFSQIGSLDRVFNLFMGMRLVG---TRPDAAT--VIGLTRAVISAKSLRF 84
           NI SWN  I GFS+  +    F L+  M   G   +RPD  T  V+    A +   SL  
Sbjct: 117 NIFSWNVTIRGFSESENPKESFLLYKQMLRHGCCESRPDHFTYPVLFKVCADLRLSSLGH 176

Query: 85  LKAVHAIGIETGLDADTSVSNTWIAAYSKCGELQLAKMVFHGIQKTARSSVSWNSLIACY 144
           +   H + +   L+  + V N  I  ++ CG+++ A+ VF   +   R  VSWN LI  Y
Sbjct: 177 MILGHVLKLR--LELVSHVHNASIHMFASCGDMENARKVFD--ESPVRDLVSWNCLINGY 236

Query: 145 AHFGKYVDAVKSYKGLLCDGFKPDARCDSDISLINTLISMYSSGYSEVGRVDDALVLFNA 204
              G+   A+  YK +  +G KPD     D+++I  +     S  S +G ++     +  
Sbjct: 237 KKIGEAEKAIYVYKLMESEGVKPD-----DVTMIGLV-----SSCSMLGDLNRGKEFYEY 296

Query: 205 MEETGEKPDIVTVLSLISGCGKTGALGLGHWI-DNYASLHELKKDVVVCNALIDMYAKCG 264
           ++E G +  I  V +L+    K G +     I DN       K+ +V    +I  YA+CG
Sbjct: 297 VKENGLRMTIPLVNALMDMFSKCGDIHEARRIFDNLE-----KRTIVSWTTMISGYARCG 356

Query: 265 SLNDAREIFYSLPNRTVVSWTAMIAACALNGEFREALDLFSLLSESGIEPNNITLTRAYG 323
            L+ +R++F  +  + VV W AMI         ++AL LF  +  S  +P+ IT+     
Sbjct: 357 LLDVSRKLFDDMEEKDVVLWNAMIGGSVQAKRGQDALALFQEMQTSNTKPDEITM----- 396

BLAST of Cucsa.104830 vs. Swiss-Prot
Match: PP175_ARATH (Pentatricopeptide repeat-containing protein At2g29760, chloroplastic OS=Arabidopsis thaliana GN=PCMP-H33 PE=2 SV=1)

HSP 1 Score: 175.6 bits (444), Expect = 1.1e-42
Identity = 97/318 (30.50%), Postives = 172/318 (54.09%), Query Frame = 1

Query: 1   MVDMYVKCGKVDDAYNLFDKMPVRNIASWNAMIIGFSQIGSLDRVFNLFMGMRLVGTRPD 60
           ++  Y  CG +D A  +F  +  +++ SWN+MI GF Q GS D+   LF  M     +  
Sbjct: 172 LIHCYFSCGDLDSACKVFTTIKEKDVVSWNSMINGFVQKGSPDKALELFKKMESEDVKAS 231

Query: 61  AATVIGLTRAVISAKSLRFLKAVHAIGIETGLDADTSVSNTWIAAYSKCGELQLAKMVFH 120
             T++G+  A    ++L F + V +   E  ++ + +++N  +  Y+KCG ++ AK +F 
Sbjct: 232 HVTMVGVLSACAKIRNLEFGRQVCSYIEENRVNVNLTLANAMLDMYTKCGSIEDAKRLFD 291

Query: 121 GIQKTARSSVSWNSLIACYAHFGKYVDAVKSYKGLLCDGFKPDARCDSDISLINTLISMY 180
            +++  + +V+W +++  YA    Y  A +    +             DI   N LIS Y
Sbjct: 292 AMEE--KDNVTWTTMLDGYAISEDYEAAREVLNSMP----------QKDIVAWNALISAY 351

Query: 181 SSGYSEVGRVDDALVLFNAME-ETGEKPDIVTVLSLISGCGKTGALGLGHWIDNYASLHE 240
                  G+ ++AL++F+ ++ +   K + +T++S +S C + GAL LG WI +Y   H 
Sbjct: 352 EQN----GKPNEALIVFHELQLQKNMKLNQITLVSTLSACAQVGALELGRWIHSYIKKHG 411

Query: 241 LKKDVVVCNALIDMYAKCGSLNDAREIFYSLPNRTVVSWTAMIAACALNGEFREALDLFS 300
           ++ +  V +ALI MY+KCG L  +RE+F S+  R V  W+AMI   A++G   EA+D+F 
Sbjct: 412 IRMNFHVTSALIHMYSKCGDLEKSREVFNSVEKRDVFVWSAMIGGLAMHGCGNEAVDMFY 471

Query: 301 LLSESGIEPNNITLTRAY 318
            + E+ ++PN +T T  +
Sbjct: 472 KMQEANVKPNGVTFTNVF 473


HSP 2 Score: 104.0 bits (258), Expect = 4.2e-21
Identity = 103/441 (23.36%), Postives = 189/441 (42.86%), Query Frame = 1

Query: 1   MVDMYVKCGKVDDAYNLFDKMPVRNIASWNAMIIGFSQIGSLDRVFNLFMGMRLVGTRPD 60
           M+DMY KCG ++DA  LFD M  ++  +W  M+ G++     +    +   M      P 
Sbjct: 273 MLDMYTKCGSIEDAKRLFDAMEEKDNVTWTTMLDGYAISEDYEAAREVLNSM------PQ 332

Query: 61  AATVIGLTRAVISA-----KSLRFLKAVHAIGIETGLDADTSVSNTWIAAYSKCGELQLA 120
              V     A+ISA     K    L   H + ++  +  +     + ++A ++ G L+L 
Sbjct: 333 KDIVAW--NALISAYEQNGKPNEALIVFHELQLQKNMKLNQITLVSTLSACAQVGALELG 392

Query: 121 KMVF-----HGIQKTARSSVSWNSLIACYAHFGKYVDAVKSYKGLLCDGFKPDARCDSDI 180
           + +      HGI+     +   ++LI  Y+  G    + + +  +             D+
Sbjct: 393 RWIHSYIKKHGIRMNFHVT---SALIHMYSKCGDLEKSREVFNSVE----------KRDV 452

Query: 181 SLINTLISMYSSGYSEVGRVDDALVLFNAMEETGEKPDIVTVLSLISGCGKTG----ALG 240
            + + +I     G +  G  ++A+ +F  M+E   KP+ VT  ++   C  TG    A  
Sbjct: 453 FVWSAMIG----GLAMHGCGNEAVDMFYKMQEANVKPNGVTFTNVFCACSHTGLVDEAES 512

Query: 241 LGHWID-NYASLHELKKDVVVCNALIDMYAKCGSLNDAREIFYSLPNRTVVS-WTAMIAA 300
           L H ++ NY  + E K    +    +D+  + G L  A +   ++P     S W A++ A
Sbjct: 513 LFHQMESNYGIVPEEKHYACI----VDVLGRSGYLEKAVKFIEAMPIPPSTSVWGALLGA 572

Query: 301 CALNGEFREALDLFSLLSESGIEPNNITLTRAYGVHCLVLVRFTTTWKSPRVAVSFVEMA 360
           C ++     A    + L E  +EP N       G H L                    ++
Sbjct: 573 CKIHANLNLAEMACTRLLE--LEPRND------GAHVL--------------------LS 632

Query: 361 NIYASVGRWDEVAAMRKTMRSNQMRKSPGKSVVQVNGMSHVFFVEDRSHHDSLLIYEALG 420
           NIYA +G+W+ V+ +RK MR   ++K PG S ++++GM H F   D +H  S  +Y  L 
Sbjct: 633 NIYAKLGKWENVSELRKHMRVTGLKKEPGCSSIEIDGMIHEFLSGDNAHPMSEKVYGKLH 656

Query: 421 NLAMQMKQKEFSSHAQRWVEL 426
            +  ++K   +     + +++
Sbjct: 693 EVMEKLKSNGYEPEISQVLQI 656


HSP 3 Score: 93.6 bits (231), Expect = 5.7e-18
Identity = 83/316 (26.27%), Postives = 145/316 (45.89%), Query Frame = 1

Query: 14  AYNLFDKMPVRNIASWNAMIIGFSQIGSLDRVFNLFMGMRLVGTR---PDAATVIGLTRA 73
           A  +FD++P  N  +WN +I  ++     D V +++  + +V      P+  T   L +A
Sbjct: 83  ARKVFDEIPKPNSFAWNTLIRAYAS--GPDPVLSIWAFLDMVSESQCYPNKYTFPFLIKA 142

Query: 74  VISAKSLRFLKAVHAIGIETGLDADTSVSNTWIAAYSKCGELQLAKMVFHGIQKTARSSV 133
                SL   +++H + +++ + +D  V+N+ I  Y  CG+L  A  VF  I++  +  V
Sbjct: 143 AAEVSSLSLGQSLHGMAVKSAVGSDVFVANSLIHCYFSCGDLDSACKVFTTIKE--KDVV 202

Query: 134 SWNSLIACYAHFGKYVDAVKSYKGLLCDGFKPDARCDSDISLINTLISMYS--------- 193
           SWNS+I  +   G    A++ +K +  +  K      S ++++  L +            
Sbjct: 203 SWNSMINGFVQKGSPDKALELFKKMESEDVKA-----SHVTMVGVLSACAKIRNLEFGRQ 262

Query: 194 -SGYSEVGRVDDALVLFNAMEETGEKPDIVTVLSLISGCGKTGALGLGHWIDNYASLHEL 253
              Y E  RV+  L L NAM            L + + CG          I++   L + 
Sbjct: 263 VCSYIEENRVNVNLTLANAM------------LDMYTKCGS---------IEDAKRLFDA 322

Query: 254 --KKDVVVCNALIDMYAKCGSLNDAREIFYSLPNRTVVSWTAMIAACALNGEFREALDLF 313
             +KD V    ++D YA       ARE+  S+P + +V+W A+I+A   NG+  EAL +F
Sbjct: 323 MEEKDNVTWTTMLDGYAISEDYEAAREVLNSMPQKDIVAWNALISAYEQNGKPNEALIVF 368

BLAST of Cucsa.104830 vs. Swiss-Prot
Match: PP311_ARATH (Pentatricopeptide repeat-containing protein At4g14820 OS=Arabidopsis thaliana GN=PCMP-H3 PE=2 SV=1)

HSP 1 Score: 171.0 bits (432), Expect = 2.8e-41
Identity = 96/312 (30.77%), Postives = 168/312 (53.85%), Query Frame = 1

Query: 2   VDMYVKCGKVDDAYNLFDKMPVRNIASWNAMIIGFSQIGSLDRVFNLFMGMRLVGTRPDA 61
           +DMY  CG+++ A N+FD+M  R++ +WN MI  + + G +D  F LF  M+     PD 
Sbjct: 153 MDMYASCGRINYARNVFDEMSHRDVVTWNTMIERYCRFGLVDEAFKLFEEMKDSNVMPDE 212

Query: 62  ATVIGLTRAVISAKSLRFLKAVHAIGIETGLDADTSVSNTWIAAYSKCGELQLAKMVFHG 121
             +  +  A     ++R+ +A++   IE  +  DT +    +  Y+  G + +A+  F  
Sbjct: 213 MILCNIVSACGRTGNMRYNRAIYEFLIENDVRMDTHLLTALVTMYAGAGCMDMAREFFR- 272

Query: 122 IQKTARSSVSWNSLIACYAHFGKYVDAVKSYKGLLCDGFKPDARCDSDISLINTLISMY- 181
            + + R+     ++++ Y+  G+  DA   +          D     D+    T+IS Y 
Sbjct: 273 -KMSVRNLFVSTAMVSGYSKCGRLDDAQVIF----------DQTEKKDLVCWTTMISAYV 332

Query: 182 SSGYSEVGRVDDALVLFNAMEETGEKPDIVTVLSLISGCGKTGALGLGHWIDNYASLHEL 241
            S Y +     +AL +F  M  +G KPD+V++ S+IS C   G L    W+ +   ++ L
Sbjct: 333 ESDYPQ-----EALRVFEEMCCSGIKPDVVSMFSVISACANLGILDKAKWVHSCIHVNGL 392

Query: 242 KKDVVVCNALIDMYAKCGSLNDAREIFYSLPNRTVVSWTAMIAACALNGEFREALDLFSL 301
           + ++ + NALI+MYAKCG L+  R++F  +P R VVSW++MI A +++GE  +AL LF+ 
Sbjct: 393 ESELSINNALINMYAKCGGLDATRDVFEKMPRRNVVSWSSMINALSMHGEASDALSLFAR 447

Query: 302 LSESGIEPNNIT 313
           + +  +EPN +T
Sbjct: 453 MKQENVEPNEVT 447


HSP 2 Score: 112.8 bits (281), Expect = 9.0e-24
Identity = 96/412 (23.30%), Postives = 172/412 (41.75%), Query Frame = 1

Query: 1   MVDMYVKCGKVDDAYNLFDKMPVRNIASWNAMIIGFSQIGSLDRVFNLFMGMRLVGTRPD 60
           MV  Y KCG++DDA  +FD+   +++  W  MI  + +         +F  M   G +PD
Sbjct: 284 MVSGYSKCGRLDDAQVIFDQTEKKDLVCWTTMISAYVESDYPQEALRVFEEMCCSGIKPD 343

Query: 61  AATVIGLTRAVISAKSLRFLKAVHAIGIETGLDADTSVSNTWIAAYSKCGELQLAKMVFH 120
             ++  +  A  +   L   K VH+     GL+++ S++N  I  Y+KCG L   + VF 
Sbjct: 344 VVSMFSVISACANLGILDKAKWVHSCIHVNGLESELSINNALINMYAKCGGLDATRDVFE 403

Query: 121 GIQKTARSSVSWNSLIACYAHFGKYVDAVKSYKGLLCDGFKPDARCDSDISLINTLISMY 180
            + +  R+ VSW+S+I   +  G+  DA+  +  +  +  +P     ++++ +  L    
Sbjct: 404 KMPR--RNVVSWSSMINALSMHGEASDALSLFARMKQENVEP-----NEVTFVGVLY--- 463

Query: 181 SSGYSEVGRVDDALVLFNAM-EETGEKPDIVTVLSLISGCGKTGALGLGHWIDNYASLHE 240
             G S  G V++   +F +M +E    P +     ++   G+   L          +L  
Sbjct: 464 --GCSHSGLVEEGKKIFASMTDEYNITPKLEHYGCMVDLFGRANLL--------REALEV 523

Query: 241 LKKDVVVCNALIDMYAKCGSLNDAREIFYSLPNRTVVSWTAMIAACALNGEFREALDLFS 300
           ++   V  N +I                          W ++++AC ++GE    L  F+
Sbjct: 524 IESMPVASNVVI--------------------------WGSLMSACRIHGELE--LGKFA 583

Query: 301 LLSESGIEPNNITLTRAYGVHCLVLVRFTTTWKSPRVAVSFVEMANIYASVGRWDEVAAM 360
                 +EP++                            + V M+NIYA   RW++V  +
Sbjct: 584 AKRILELEPDH--------------------------DGALVLMSNIYAREQRWEDVRNI 621

Query: 361 RKTMRSNQMRKSPGKSVVQVNGMSHVFFVEDRSHHDSLLIYEALGNLAMQMK 412
           R+ M    + K  G S +  NG SH F + D+ H  S  IY  L  +  ++K
Sbjct: 644 RRVMEEKNVFKEKGLSRIDQNGKSHEFLIGDKRHKQSNEIYAKLDEVVSKLK 621


HSP 3 Score: 87.8 bits (216), Expect = 3.1e-16
Identity = 83/378 (21.96%), Postives = 156/378 (41.27%), Query Frame = 1

Query: 14  AYNLFDKMPVR-NIASWNAMIIGFSQIGSLDRVFNLFMGMRLVGTRPDAATVIGLTRAVI 73
           A N+F  +P       +N  +   S+          +  +R VG R D  + + + +AV 
Sbjct: 63  ALNVFSSIPSPPESIVFNPFLRDLSRSSEPRATILFYQRIRHVGGRLDQFSFLPILKAVS 122

Query: 74  SAKSLRFLKAVHAIGIETGLDADTSVSNTWIAAYSKCGELQLAKMVFHGIQKTARSSVSW 133
              +L     +H +  +     D  V   ++  Y+ CG +  A+ VF   + + R  V+W
Sbjct: 123 KVSALFEGMELHGVAFKIATLCDPFVETGFMDMYASCGRINYARNVFD--EMSHRDVVTW 182

Query: 134 NSLIACYAHFGKYVDAVKSYKGLLCDGFKPDARCDSDISLINTLISMYSSGYSEVGRVDD 193
           N++I  Y  FG   +A K ++ +      PD     ++ L N + +   +G     R   
Sbjct: 183 NTMIERYCRFGLVDEAFKLFEEMKDSNVMPD-----EMILCNIVSACGRTGNMRYNRA-- 242

Query: 194 ALVLFNAMEETGEKPDIVTVLSLISGCGKTGALGLGHWIDNYASLHELKKDVVVCNALID 253
              ++  + E   + D   + +L++     G + +        S+  L     V  A++ 
Sbjct: 243 ---IYEFLIENDVRMDTHLLTALVTMYAGAGCMDMAREFFRKMSVRNL----FVSTAMVS 302

Query: 254 MYAKCGSLNDAREIFYSLPNRTVVSWTAMIAACALNGEFREALDLFSLLSESGIEPNNIT 313
            Y+KCG L+DA+ IF     + +V WT MI+A   +   +EAL +F  +  SGI+P+ ++
Sbjct: 303 GYSKCGRLDDAQVIFDQTEKKDLVCWTTMISAYVESDYPQEALRVFEEMCCSGIKPDVVS 362

Query: 314 LTRAYGVHCLVLVRFTTTWKSPRVAVSFVE--------MANIYASVGRWDEVAAMRKTMR 373
           +         + +     W    + V+ +E        + N+YA  G  D          
Sbjct: 363 MFSVISACANLGILDKAKWVHSCIHVNGLESELSINNALINMYAKCGGLDAT-------- 416

Query: 374 SNQMRKSPGKSVVQVNGM 383
            +   K P ++VV  + M
Sbjct: 423 RDVFEKMPRRNVVSWSSM 416

BLAST of Cucsa.104830 vs. Swiss-Prot
Match: PP235_ARATH (Putative pentatricopeptide repeat-containing protein At3g15930 OS=Arabidopsis thaliana GN=PCMP-E51 PE=3 SV=2)

HSP 1 Score: 164.1 bits (414), Expect = 3.4e-39
Identity = 96/314 (30.57%), Postives = 158/314 (50.32%), Query Frame = 1

Query: 1   MVDMYVKCGKVDDAYNLFDKMPVRNIASWNAMIIGFSQIGSLDRVFNLFMGMRLVGTRPD 60
           +V MY  CG +D A  +FD+    ++ SWN MI G++++   +    L + M      P 
Sbjct: 176 LVKMYSLCGLMDMARGVFDRRCKEDVFSWNLMISGYNRMKEYEESIELLVEMERNLVSPT 235

Query: 61  AATVIGLTRAVISAKSLRFLKAVHAIGIETGLDADTSVSNTWIAAYSKCGELQLAKMVFH 120
           + T++ +  A    K     K VH    E   +    + N  + AY+ CGE+ +A  +F 
Sbjct: 236 SVTLLLVLSACSKVKDKDLCKRVHEYVSECKTEPSLRLENALVNAYAACGEMDIAVRIFR 295

Query: 121 GIQKTARSSVSWNSLIACYAHFGKYVDAVKSYKGLLCDGFKPDARCDSDISLINTLIS-- 180
            ++  AR  +SW S++                KG +  G    AR   D   +   IS  
Sbjct: 296 SMK--ARDVISWTSIV----------------KGYVERGNLKLARTYFDQMPVRDRISWT 355

Query: 181 MYSSGYSEVGRVDDALVLFNAMEETGEKPDIVTVLSLISGCGKTGALGLGHWIDNYASLH 240
           +   GY   G  +++L +F  M+  G  PD  T++S+++ C   G+L +G WI  Y   +
Sbjct: 356 IMIDGYLRAGCFNESLEIFREMQSAGMIPDEFTMVSVLTACAHLGSLEIGEWIKTYIDKN 415

Query: 241 ELKKDVVVCNALIDMYAKCGSLNDAREIFYSLPNRTVVSWTAMIAACALNGEFREALDLF 300
           ++K DVVV NALIDMY KCG    A+++F+ +  R   +WTAM+   A NG+ +EA+ +F
Sbjct: 416 KIKNDVVVGNALIDMYFKCGCSEKAQKVFHDMDQRDKFTWTAMVVGLANNGQGQEAIKVF 471

Query: 301 SLLSESGIEPNNIT 313
             + +  I+P++IT
Sbjct: 476 FQMQDMSIQPDDIT 471


HSP 2 Score: 107.8 bits (268), Expect = 2.9e-22
Identity = 101/413 (24.46%), Postives = 179/413 (43.34%), Query Frame = 1

Query: 1   MVDMYVKCGKVDDAYNLFDKMPVRNIASWNAMIIGFSQIGSLDRVFNLFMGMRLVGTRPD 60
           +V+ Y  CG++D A  +F  M  R++ SW +++ G+ + G+L      F  M +      
Sbjct: 277 LVNAYAACGEMDIAVRIFRSMKARDVISWTSIVKGYVERGNLKLARTYFDQMPVRDRISW 336

Query: 61  AATVIGLTRAVISAKSLRFLKAVHAIGIETGLDADTSVSNTWIAAYSKCGELQLAKMVFH 120
              + G  RA    +SL   + + + G+    D  T VS   + A +  G L++ + +  
Sbjct: 337 TIMIDGYLRAGCFNESLEIFREMQSAGMIP--DEFTMVSV--LTACAHLGSLEIGEWIKT 396

Query: 121 GIQKTARSS--VSWNSLIACYAHFGKYVDAVKSYKGLLCDGFKPDARCDSDISLINTLIS 180
            I K    +  V  N+LI  Y   G    A K +              D D     T  +
Sbjct: 397 YIDKNKIKNDVVVGNALIDMYFKCGCSEKAQKVFH-------------DMDQRDKFTWTA 456

Query: 181 MYSSGYSEVGRVDDALVLFNAMEETGEKPDIVTVLSLISGCGKTGALGLGH-WIDNYASL 240
           M   G +  G+  +A+ +F  M++   +PD +T L ++S C  +G +     +     S 
Sbjct: 457 MVV-GLANNGQGQEAIKVFFQMQDMSIQPDDITYLGVLSACNHSGMVDQARKFFAKMRSD 516

Query: 241 HELKKDVVVCNALIDMYAKCGSLNDAREIFYSLP-NRTVVSWTAMIAACALNGEFREALD 300
           H ++  +V    ++DM  + G + +A EI   +P N   + W A++ A  L+ +  E + 
Sbjct: 517 HRIEPSLVHYGCMVDMLGRAGLVKEAYEILRKMPMNPNSIVWGALLGASRLHND--EPMA 576

Query: 301 LFSLLSESGIEPNNITLTRAYGVHCLVLVRFTTTWKSPRVAVSFVEMANIYASVGRWDEV 360
             +      +EP+N  +   Y + C                       NIYA   RW ++
Sbjct: 577 ELAAKKILELEPDNGAV---YALLC-----------------------NIYAGCKRWKDL 636

Query: 361 AAMRKTMRSNQMRKSPGKSVVQVNGMSHVFFVEDRSHHDSLLIYEALGNLAMQ 410
             +R+ +    ++K+PG S+++VNG +H F   D+SH  S  IY  L  LA +
Sbjct: 637 REVRRKIVDVAIKKTPGFSLIEVNGFAHEFVAGDKSHLQSEEIYMKLEELAQE 643


HSP 3 Score: 58.2 bits (139), Expect = 2.6e-07
Identity = 41/148 (27.70%), Postives = 73/148 (49.32%), Query Frame = 1

Query: 167 DSDISLINTLISMYSSGYSEVGRVDDALVLFNAMEETGEKPDIVTVLSLISGCGKTG-AL 226
           + D+ + N +I     G+S+V    + + L+  M + G  PD  T   L++G  + G AL
Sbjct: 96  EPDVVVWNNMIK----GWSKVDCDGEGVRLYLNMLKEGVTPDSHTFPFLLNGLKRDGGAL 155

Query: 227 GLGHWIDNYASLHELKKDVVVCNALIDMYAKCGSLNDAREIFYSLPNRTVVSWTAMIAAC 286
             G  +  +     L  ++ V NAL+ MY+ CG ++ AR +F       V SW  MI+  
Sbjct: 156 ACGKKLHCHVVKFGLGSNLYVQNALVKMYSLCGLMDMARGVFDRRCKEDVFSWNLMISGY 215

Query: 287 ALNGEFREALDLFSLLSESGIEPNNITL 314
               E+ E+++L   +  + + P ++TL
Sbjct: 216 NRMKEYEESIELLVEMERNLVSPTSVTL 239

BLAST of Cucsa.104830 vs. TrEMBL
Match: A0A0A0KK94_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_6G501860 PE=4 SV=1)

HSP 1 Score: 468.8 bits (1205), Expect = 7.2e-129
Identity = 261/381 (68.50%), Postives = 275/381 (72.18%), Query Frame = 1

Query: 1   MVDMYVKCGKVDDAYNLFDKMPVRNIASWNAMIIGFSQIGSLDRVFNLFMGMRLVGTRPD 60
           MVDMYVKCGKVDDAYNLFDKMPVRNIASWNAMIIGFSQIGSLDRVFNLFMGMRLVGTRPD
Sbjct: 93  MVDMYVKCGKVDDAYNLFDKMPVRNIASWNAMIIGFSQIGSLDRVFNLFMGMRLVGTRPD 152

Query: 61  AATVIGLTRAVISAKSLRFLKAVHAIGIETGLDADTSVSNTWIAAYSKCGELQLAKMVFH 120
           AATVIGLTRAVISAKSLRFLKAVHAIGIETGLDADTSVSNTWIAAYSKCGELQLAKMVFH
Sbjct: 153 AATVIGLTRAVISAKSLRFLKAVHAIGIETGLDADTSVSNTWIAAYSKCGELQLAKMVFH 212

Query: 121 GIQKTARSSVSWNSLIACYAHFGKYVDAVKSYKGLLCDGFKPDAR--------------- 180
           GIQKTARSSVSWNSLIACYAHFGKYVDAVKSYKGLLCDGFKPDA                
Sbjct: 213 GIQKTARSSVSWNSLIACYAHFGKYVDAVKSYKGLLCDGFKPDASTIISLLSSCQQPEAL 272

Query: 181 --------------CDSDISLINTLISMYSSGYSEVGRVDDALVLFNAM----------- 240
                         CDSDISLINTLISM    YS  G +  A +LF+ M           
Sbjct: 273 IYGFLIHGHGFQLGCDSDISLINTLISM----YSRCGDISSATILFDGMSIRTCVSWTAM 332

Query: 241 ----EETGEKPDIVTVLSLISGCGKTGAL--------GLGH----WIDNYASLHELKKD- 300
                E G   D + + + +   G+   +        G G      + ++   +    + 
Sbjct: 333 ISGYSEVGRVDDALVLFNAMEETGEKPDIVTVLSLISGCGKTGALGLGHWIDNYASLHEL 392

Query: 301 ---VVVCNALIDMYAKCGSLNDAREIFYSLPNRTVVSWTAMIAACALNGEFREALDLFSL 322
              VVVCNALIDMYAKCGSLNDAREIFYSLPNRTVVSWTAMIAACALNGEFREALDLFSL
Sbjct: 393 KKDVVVCNALIDMYAKCGSLNDAREIFYSLPNRTVVSWTAMIAACALNGEFREALDLFSL 452

BLAST of Cucsa.104830 vs. TrEMBL
Match: A0A0A0KK94_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_6G501860 PE=4 SV=1)

HSP 1 Score: 232.6 bits (592), Expect = 8.7e-58
Identity = 165/432 (38.19%), Postives = 214/432 (49.54%), Query Frame = 1

Query: 1   MVDMYVKCGKVDDAYNLFDKMPVRNIASWNAMIIGFSQIGSLDRVFNLFMGMRLVGTRPD 60
           ++ MY +CG +  A  LFD M +R   SW AMI                 G   VG   D
Sbjct: 297 LISMYSRCGDISSATILFDGMSIRTCVSWTAMI----------------SGYSEVGRVDD 356

Query: 61  AATVIGLTRAVISAKSLRFLKAVHAIGIETGLDADTSVSNTWIAAYSKCGELQLAKMV-- 120
           A  +                        ETG   D     + I+   K G L L   +  
Sbjct: 357 ALVLFNAME-------------------ETGEKPDIVTVLSLISGCGKTGALGLGHWIDN 416

Query: 121 FHGIQKTARSSVSWNSLIACYAHFGKYVDAVKSYKGLLCDGFKPDARCDSDISLINTLIS 180
           +  + +  +  V  N+LI  YA  G   DA + +  L      P+    S  ++I     
Sbjct: 417 YASLHELKKDVVVCNALIDMYAKCGSLNDAREIFYSL------PNRTVVSWTAMI----- 476

Query: 181 MYSSGYSEVGRVDDALVLFNAMEETGEKPDIVTVLSLISGCGKTGALGLGH-WIDNYASL 240
              +  +  G   +AL LF+ + E+G +P+ +T L+++  C   G L  G          
Sbjct: 477 ---AACALNGEFREALDLFSLLSESGIEPNNITFLAVLQACCHGGYLEKGRECFMMMTER 536

Query: 241 HELKKDVVVCNALIDMYAKCGSLNDAREIFYSLPNRTVVS-WTAMIAACALNGEFREALD 300
           + +   +   + +ID+  + G L +A E+   +P +     W A++ AC ++        
Sbjct: 537 YGINPGLDHYSCMIDLLGRKGKLIEALEVIQDMPMKPDEGIWGALLGACKIH-------- 596

Query: 301 LFSLLSESGIEPNNITLTRAYGVHCLVLVRFTTTWKSPRVAVSFVEMANIYASVGRWDEV 360
                       NN+ +      +   L         PRVAVSFVEMANIYASVGRWDEV
Sbjct: 597 ------------NNMEIGEYVSRYLFEL--------QPRVAVSFVEMANIYASVGRWDEV 651

Query: 361 AAMRKTMRSNQMRKSPGKSVVQVNGMSHVFFVEDRSHHDSLLIYEALGNLAMQMKQKEFS 420
           AAMRKTMRSNQMRKSPGKSVVQVNGMSHVFFVEDRSHHDSLLIYEALGNLAMQMKQKEFS
Sbjct: 657 AAMRKTMRSNQMRKSPGKSVVQVNGMSHVFFVEDRSHHDSLLIYEALGNLAMQMKQKEFS 651

Query: 421 SHAQRWVELDTI 429
           SHAQRWVELDTI
Sbjct: 717 SHAQRWVELDTI 651


HSP 2 Score: 69.7 bits (169), Expect = 9.8e-09
Identity = 48/151 (31.79%), Postives = 68/151 (45.03%), Query Frame = 1

Query: 171 SLINTLISMYSS--GYSEVGRVDDALVLFNAMEETGEKPDIVTVLSLISGCGKTGALGLG 230
           S ++TL    SS  G    G    AL LF+ ++  G +P+  T   L   C K   L   
Sbjct: 12  SKLSTLTWWNSSIRGAVNQGNASKALALFHQLKLNGLQPNNFTFPFLSKACAKLSHLTNS 71

Query: 231 HWIDNYASLHELKKDVVVCNALIDMYAKCGSLNDAREIFYSLPNRTVVSWTAMIAACALN 290
             I  +        D+ V  A++DMY KCG ++DA  +F  +P R + SW AMI   +  
Sbjct: 72  QIIHTHVVKSPFYSDIYVQTAMVDMYVKCGKVDDAYNLFDKMPVRNIASWNAMIIGFSQI 131

Query: 291 GEFREALDLFSLLSESGIEPNNIT---LTRA 317
           G      +LF  +   G  P+  T   LTRA
Sbjct: 132 GSLDRVFNLFMGMRLVGTRPDAATVIGLTRA 162


HSP 3 Score: 339.0 bits (868), Expect = 8.6e-90
Identity = 175/369 (47.43%), Postives = 249/369 (67.48%), Query Frame = 1

Query: 1   MVDMYVKCGKVDDAYNLFDKMPVRNIASWNAMIIGFSQIGSLDRVFNLFMGMRLVGTRPD 60
           +VDMY+KC ++ DA+ +F+KMP R++A+WN+MI+G +Q+G  +RVF LF  MRL G + D
Sbjct: 51  VVDMYLKCDRLSDAHGVFEKMPARDVAAWNSMIVGLAQLGFTNRVFCLFHRMRLAGIQLD 110

Query: 61  AATVIGLTRAVIS-AKSLRFLKAVHAIGIETGLDADTSVSNTWIAAYSKCGELQLAKMVF 120
           + T++GLT+A+ S +K++  LKA+H++GI  GL+AD SV+NTWI+AY+KC +L  AK+VF
Sbjct: 111 SITIMGLTQAITSHSKNVELLKAIHSLGIRIGLEADISVANTWISAYAKCSDLDSAKVVF 170

Query: 121 HGIQKTARSSVSWNSLIACYAHFGK-----------YVDAVK----SYKGLLCDGFKPDA 180
            GI    R+ VSWNS+IA Y++F K            VD  +    +  GLL    +P A
Sbjct: 171 DGIDLDVRTVVSWNSMIAAYSNFEKSIDALNCYKRMLVDGYRPDSSTILGLLSSCAQPKA 230

Query: 181 R--------------CDSDISLINTLISMYS---------------------------SG 240
                          CDSDI++ NTLISMYS                           SG
Sbjct: 231 LLQGASVHCHGIQLGCDSDIAVANTLISMYSRCGDIFAARLLFDCVSCRTCVTWNVMISG 290

Query: 241 YSEVGRVDDALVLFNAMEETGEKPDIVTVLSLISGCGKTGALGLGHWIDNYASLHELKKD 300
           Y++ G +D+AL LF+AME  GEKPD+VT+LS+ISGC +TG+L +G WI+ YA  + L+ +
Sbjct: 291 YADKGDLDEALELFDAMETAGEKPDLVTMLSVISGCSQTGSLEVGKWINGYAFSNGLRDN 350

Query: 301 VVVCNALIDMYAKCGSLNDAREIFYSLPNRTVVSWTAMIAACALNGEFREALDLFSLLSE 313
            ++CNALIDMYAKCGS+NDAR++FY+LP +TVVSWT +I+ CALNGE +EAL+LF+ + E
Sbjct: 351 TIICNALIDMYAKCGSMNDARKLFYALPEKTVVSWTTIISGCALNGEVKEALNLFNRMLE 410

BLAST of Cucsa.104830 vs. TrEMBL
Match: W9RJG3_9ROSA (Uncharacterized protein OS=Morus notabilis GN=L484_020091 PE=4 SV=1)

HSP 1 Score: 181.8 bits (460), Expect = 1.8e-42
Identity = 121/417 (29.02%), Postives = 198/417 (47.48%), Query Frame = 1

Query: 1   MVDMYVKCGKVDDAYNLFDKMPVRNIASWNAMIIGFSQIGSLDRVFNLFMGMRLVGTRPD 60
           ++ MY +CG +  A  LFD +  R   +WN MI G++  G LD    LF  M   G +PD
Sbjct: 256 LISMYSRCGDIFAARLLFDCVSCRTCVTWNVMISGYADKGDLDEALELFDAMETAGEKPD 315

Query: 61  AATVIGLTRAVISAKSLRFLKAVHAIGIETGLDADTSVSNTWIAAYSKCGELQLAKMVFH 120
             T++ +        SL   K ++      GL  +T + N  I  Y+KCG +  A+ +F+
Sbjct: 316 LVTMLSVISGCSQTGSLEVGKWINGYAFSNGLRDNTIICNALIDMYAKCGSMNDARKLFY 375

Query: 121 GIQKTARSSVSWNSLIACYAHFGKYVDAVKSYKGLLCDGFKPDARCDSDISLINTLISMY 180
            + +  ++ VSW ++I+  A  G+  +A+  +  +L  G KP     + ++ +  L +  
Sbjct: 376 ALPE--KTVVSWTTIISGCALNGEVKEALNLFNRMLESGLKP-----NHVTFLAVLQACA 435

Query: 181 SSGYSEVGRVDDALVLFNAMEETGEKPDIVTVLSLISGCGKTGALGLGHWIDNYASLHEL 240
            +G  E G                E  D++T +  I+ C           +D+Y      
Sbjct: 436 HAGLLEKGL---------------ECFDVMTKVFRINPC-----------LDHY------ 495

Query: 241 KKDVVVCNALIDMYAKCGSLNDAREIFYSLPNRTVVS-WTAMIAACALNGEFREALDLFS 300
                  + ++D+  + G + +A E+  ++P +     W+++++AC ++           
Sbjct: 496 -------SCVVDLLGRKGKIREAFELLENMPLKPDAGVWSSLLSACKIH----------- 555

Query: 301 LLSESGIEPNNITLTRAYGVHCLVLVRFTTTWKSPRVAVSFVEMANIYASVGRWDEVAAM 360
                     NI +      H   L         P  AV +VEMANIYAS G+WD VAA+
Sbjct: 556 ---------QNIEIAEYVSCHLFEL--------DPLAAVPYVEMANIYASDGKWDGVAAI 598

Query: 361 RKTMRSNQMRKSPGKSVVQVNGMSHVFFVEDRSHHDSLLIYEALGNLAMQMKQKEFS 417
           RK M+ N ++KS GKS+VQVNG  HVF VEDR H +SL IYE L +L + +K+ + S
Sbjct: 616 RKMMKCNSVKKSAGKSIVQVNGKRHVFAVEDRGHSESLCIYEMLDSLMLLLKEGDLS 598


HSP 2 Score: 299.7 bits (766), Expect = 5.8e-78
Identity = 160/369 (43.36%), Postives = 223/369 (60.43%), Query Frame = 1

Query: 1   MVDMYVKCGKVDD-AYNLFDKMPVRNIASWNAMIIGFSQIGSLDRVFNLFMGMRLVGTRP 60
           +++MYVKC +V++ AY +FD+M  R IA+WNAM++GF Q    DR   LF  M + G RP
Sbjct: 97  LLNMYVKCDEVENFAYAVFDRMHAREIAAWNAMLMGFVQASCFDRALCLFNEMMVDGVRP 156

Query: 61  DAATVIGLTRAVISAKSLRFLKAVHAIGIETGLDADTSVSNTWIAAYSKCGELQLAKMVF 120
           D+ TV+GL +     K ++ L +VH +GI  G++AD SV+NTWIAAYS+CG+L L++MVF
Sbjct: 157 DSVTVMGLAQLASGLKDVKLLTSVHCLGIRIGVEADVSVANTWIAAYSRCGDLSLSEMVF 216

Query: 121 HGIQKTARSSVSWNSLIACYAHFGKYVDAVKSYKGLLCDGFKPDAR-------------- 180
           + I     + VSWNS+I   A+ G+   A++ Y  +L  GF+PD                
Sbjct: 217 NEIDLDVLTVVSWNSMITGCANAGEGFMAMELYHNMLYGGFRPDLSTILNLLGSFAKPNA 276

Query: 181 ---------------CDSDISLINTLISMYS---------------------------SG 240
                          C SD+S++NTL+ MYS                             
Sbjct: 277 LLLGKLVHSHGVQLGCCSDVSVLNTLVCMYSRCADIDSARSLFDSIVDRTRVSWTVMIGS 336

Query: 241 YSEVGRVDDALVLFNAMEETGEKPDIVTVLSLISGCGKTGALGLGHWIDNYASLHELKKD 300
           Y+E G ++DA+ LF+AME  GE+PD+VTV+ LIS CG+ GAL  G WID+YA    LK D
Sbjct: 337 YAEKGDLEDAMALFHAMEAAGEQPDLVTVIYLISACGQVGALDYGRWIDSYAISRGLKSD 396

Query: 301 VVVCNALIDMYAKCGSLNDAREIFYSLPNRTVVSWTAMIAACALNGEFREALDLFSLLSE 313
           + V NAL+DMY+KCGS  DA E+F S+  RT+VSWTAMI+  ALNG+  +ALD F+++  
Sbjct: 397 IRVSNALLDMYSKCGSARDAEELFSSMNERTIVSWTAMISGYALNGQSGKALDYFNVMLM 456

BLAST of Cucsa.104830 vs. TrEMBL
Match: A0A068UX59_COFCA (Uncharacterized protein OS=Coffea canephora GN=GSCOC_T00037516001 PE=4 SV=1)

HSP 1 Score: 130.6 bits (327), Expect = 4.7e-27
Identity = 111/418 (26.56%), Postives = 182/418 (43.54%), Query Frame = 1

Query: 1   MVDMYVKCGKVDDAYNLFDKMPVRNIASWNAMIIGFSQIGSLDRVFNLFMGMRLVGTRPD 60
           +V MY +C  +D A +LFD +  R   SW  MI  +++ G L+                D
Sbjct: 302 LVCMYSRCADIDSARSLFDSIVDRTRVSWTVMIGSYAEKGDLE----------------D 361

Query: 61  AATVIGLTRAVISAKSL-RFLKAVHAIGIETGLDADTSVSNTWIAAYSKCGELQLAKMVF 120
           A  +     A      L   +  + A G    LD        WI +Y+          + 
Sbjct: 362 AMALFHAMEAAGEQPDLVTVIYLISACGQVGALDY-----GRWIDSYA----------IS 421

Query: 121 HGIQKTARSSVSWNSLIACYAHFGKYVDAVKSYKGLLCDGFKPDARCDSDISLINTLISM 180
            G++   R S   N+L+  Y+  G   DA + +  +            ++ ++++    +
Sbjct: 422 RGLKSDIRVS---NALLDMYSKCGSARDAEELFSSM------------NERTIVSWTAMI 481

Query: 181 YSSGYSEVGRVDDALVLFNAMEETGEKPDIVTVLSLISGCGKTGALGLG-HWIDNYASLH 240
             SGY+  G+   AL  FN M  +G +P+ +T L+++  C   G +  G         L+
Sbjct: 482 --SGYALNGQSGKALDYFNVMLMSGLEPNHITFLAVLQACVHAGLMEKGWELFHRMTELY 541

Query: 241 ELKKDVVVCNALIDMYAKCGSLNDAREIFYSLPNRTVVS-WTAMIAACAL--NGEFREAL 300
           +L   +     + D+  + G L +A E    +P +     W A+++AC +  N +  E  
Sbjct: 542 KLSPGLDHHACMADLLGRQGKLKEALEYIQCMPIKPDAGIWAALLSACKIHRNADIGE-- 601

Query: 301 DLFSLLSESGIEPNNITLTRAYGVHCLVLVRFTTTWKSPRVAVSFVEMANIYASVGRWDE 360
                                Y  H L  +        P+ A  +VEMANIYAS GRWD 
Sbjct: 602 ---------------------YAAHHLFELE-------PQAAAPYVEMANIYASTGRWDG 641

Query: 361 VAAMRKTMRSNQMRKSPGKSVVQVNGMSHVFFVEDRSHHDSLLIYEALGNLAMQMKQK 414
           VA++R  M+SNQ+RK PG+S +QV+G S+ F V+D+ H    LIYE L +L +  K++
Sbjct: 662 VASIRTKMKSNQVRKYPGESTIQVDGKSYTFRVDDKFHPKGCLIYEVLSSLGLYSKKQ 641


HSP 2 Score: 296.6 bits (758), Expect = 4.9e-77
Identity = 158/369 (42.82%), Postives = 225/369 (60.98%), Query Frame = 1

Query: 1   MVDMYVKCGKVDDAYNLFDKMPVRNIASWNAMIIGFSQIGSLDRVFNLFMGMRLVGTRPD 60
           ++DMYVKC K++ A+ +FD MP R++  WNAM++GF+ +GS+D  F LF  MRL G  PD
Sbjct: 95  VIDMYVKCNKLNLAHQVFDNMPERDVTMWNAMLLGFAHLGSVDGFFVLFQEMRLHGFIPD 154

Query: 61  AATVIGLTRAVISAKSLRFLKAVHAIGIETGLDADTSVSNTWIAAYSKCGELQLAKMVFH 120
             T+IGLT++VI  K L  ++  HA GI  G+  + S++NT I+ YSKCG+L  AK +F 
Sbjct: 155 LITIIGLTQSVIELKDLNLVRGSHAFGIRFGMINNVSLANTLISGYSKCGDLVSAKCMFD 214

Query: 121 GIQKTARSSVSWNSLIACYAHFGKYVDAVKSYKGLLCD----------------GFKPDA 180
            I   +R++VSWNSLIA +A    + +    Y+ +LC+                   P+A
Sbjct: 215 TICFESRTNVSWNSLIAGHALIRNHNETFNLYRCMLCNDGRKPDISTFLSVLSSCVLPEA 274

Query: 181 R--------------CDSDISLINTLISMYSS---------------------------G 240
                          CD D++L NTLI+MYS                            G
Sbjct: 275 LGYGKLVHSHVIQLGCDYDVTLGNTLITMYSKCGDIDSARHLFHNMTYKTCITWTAMIGG 334

Query: 241 YSEVGRVDDALVLFNAMEETGEKPDIVTVLSLISGCGKTGALGLGHWIDNYASLHELKKD 300
           Y+  G +D A  LF  M+  GE PD+VT+LSLI+GCG+TG+L LG W++NY     L+  
Sbjct: 335 YAANGSLDKAYSLFFDMQAAGETPDVVTLLSLIAGCGRTGSLELGRWLNNYGISKGLRDS 394

Query: 301 VVVCNALIDMYAKCGSLNDAREIFYSLPNRTVVSWTAMIAACALNGEFREALDLFSLLSE 313
           +VVCNA+IDMYAKCGSL++A+++F+S+P++T+VSWT MI+  A+NGEF EAL+LF  + E
Sbjct: 395 IVVCNAMIDMYAKCGSLDEAKKLFHSMPSKTIVSWTTMISGLAMNGEFTEALELFLKMIE 454

BLAST of Cucsa.104830 vs. TrEMBL
Match: A0A0J8BD82_BETVU (Uncharacterized protein OS=Beta vulgaris subsp. vulgaris GN=BVRB_4g097410 PE=4 SV=1)

HSP 1 Score: 132.1 bits (331), Expect = 1.6e-27
Identity = 114/393 (29.01%), Postives = 176/393 (44.78%), Query Frame = 1

Query: 30  NAMIIGFSQIGSLDRVFNLFMGMRLVGTRPDAATVIGLTRAVISAKSLRFLKAVHAIGIE 89
           N +I  +S+ G +D   +LF  M         A + G        K+      + A G E
Sbjct: 298 NTLITMYSKCGDIDSARHLFHNMTYKTCITWTAMIGGYAANGSLDKAYSLFFDMQAAG-E 357

Query: 90  TGLDADTSVSNTWIAAYSKCGELQLAKMVF-HGIQKTARSS-VSWNSLIACYAHFGKYVD 149
           T    D     + IA   + G L+L + +  +GI K  R S V  N++I  YA  G   +
Sbjct: 358 T---PDVVTLLSLIAGCGRTGSLELGRWLNNYGISKGLRDSIVVCNAMIDMYAKCGSLDE 417

Query: 150 AVKSYKGLLCDGFKPDARCDSDISLINTLISMYSSGYSEVGRVDDALVLFNAMEETGEKP 209
           A K +  +      P     S  ++I        SG +  G   +AL LF  M E G +P
Sbjct: 418 AKKLFHSM------PSKTIVSWTTMI--------SGLAMNGEFTEALELFLKMIEQGVEP 477

Query: 210 DIVTVLSLISGCGKTGALGLG-HWIDNYASLHELKKDVVVCNALIDMYAKCGSLNDAREI 269
           + +T L+++  C   G L  G    D    +++L   +   + ++D+  + G   +A E 
Sbjct: 478 NHITFLAVLQACIHAGFLDEGMECFDLMTRVYKLNPGLEHYSCIVDLLGRKGKFKEALEF 537

Query: 270 FYSLP-NRTVVSWTAMIAACALNGEFREALDLFSLLSESGIEPNNITLTRAYGVHCLVLV 329
             S+P +     W A++ AC +                      N+ +      H   L 
Sbjct: 538 IKSMPFDPDAAIWGALLGACKIY--------------------KNVDIGEYAAYHLYKL- 597

Query: 330 RFTTTWKSPRVAVSFVEMANIYASVGRWDEVAAMRKTMRSNQMRKSPGKSVVQVNGMSHV 389
                   P  AVS+VEMANIYAS GRWD V+ +R  M+ N+++KSPG+S++QV+G +H+
Sbjct: 598 -------EPEAAVSYVEMANIYASAGRWDGVSKVRALMKCNKVKKSPGQSLIQVDGKNHI 644

Query: 390 FFVEDRSHHDSLLIYEALGNLAMQMKQKEFSSH 419
           F VE+R H     I+E L  LA Q+KQ   S H
Sbjct: 658 FGVEERCHPVGSQIFELLDVLAFQLKQAGVSYH 644


HSP 2 Score: 64.7 bits (156), Expect = 3.1e-07
Identity = 39/121 (32.23%), Postives = 56/121 (46.28%), Query Frame = 1

Query: 193 ALVLFNAMEETGEKPDIVTVLSLISGCGKTGALGLGHWIDNYASLHELKKDVVVCNALID 252
           AL L+  M++ G  PD +T+  +   C K   L     I  +        D+ V  A+ID
Sbjct: 38  ALTLYRLMKQAGLIPDKLTLPFVAKACAKISDLKQSQIIHAHIFKSPFWSDIFVGTAVID 97

Query: 253 MYAKCGSLNDAREIFYSLPNRTVVSWTAMIAACALNGEFREALDLFSLLSESGIEPNNIT 312
           MY KC  LN A ++F ++P R V  W AM+   A  G       LF  +   G  P+ IT
Sbjct: 98  MYVKCNKLNLAHQVFDNMPERDVTMWNAMLLGFAHLGSVDGFFVLFQEMRLHGFIPDLIT 157

Query: 313 L 314
           +
Sbjct: 158 I 158


HSP 3 Score: 61.6 bits (148), Expect = 2.7e-06
Identity = 29/74 (39.19%), Postives = 39/74 (52.70%), Query Frame = 1

Query: 1   MVDMYVKCGKVDDAYNLFDKMPVRNIASWNAMIIGFSQIGSLDRVFNLFMGMRLVGTRPD 60
           M+DMY KCG +D+A  LF  MP + I SW  MI G +  G       LF+ M   G  P+
Sbjct: 401 MIDMYAKCGSLDEAKKLFHSMPSKTIVSWTTMISGLAMNGEFTEALELFLKMIEQGVEPN 460

Query: 61  AATVIGLTRAVISA 75
             T + + +A I A
Sbjct: 461 HITFLAVLQACIHA 474


HSP 4 Score: 277.3 bits (708), Expect = 3.1e-71
Identity = 160/377 (42.44%), Postives = 223/377 (59.15%), Query Frame = 1

Query: 1   MVDMYVKCGKVDDAYNLFDKMPVRNIASWNAMIIGFSQIGSLDRVFNLFMGMRLVGTRPD 60
           +VDMYVKC ++  AYNLF +MP R++ASWN+MI+GF+Q+G +DRV +LF  M + G R D
Sbjct: 95  VVDMYVKCSQLGFAYNLFSRMPKRDVASWNSMILGFAQLGFVDRVVSLFCEMGIEGIRAD 154

Query: 61  AATVIGLTRAVISAKSLRFLKAVH----AIGIET-------------------------- 120
           + TVIGLT + +S K L+ L+++H     IGI+T                          
Sbjct: 155 SVTVIGLTHSALSLKDLKMLESIHSFGIKIGIDTDVSVSNTWIAAYAKCGEFGLAETVFD 214

Query: 121 GLDADTSVSNTW---IAAYSKCGELQLA-----KMVFHGIQKTARSSVSWNSLIACYAHF 180
           G+D       +W   IA Y+   +   A     KM+  G +  A  S   + L +C    
Sbjct: 215 GIDKGLKTGVSWNSMIAGYAHFEQCSKAVGFFKKMLCGGFR--ADLSTILSLLSSC---- 274

Query: 181 GKYVDAVKSYKGLLCDGFKPDARCDSDISLINTLISMYS--------------------- 240
              V     + G L         CDSDI +INTLISMYS                     
Sbjct: 275 ---VQPEVLFHGKLIHAHGIQVGCDSDIQVINTLISMYSKCGDIGSARYLFDNMLGKTRV 334

Query: 241 ------SGYSEVGRVDDALVLFNAMEETGEKPDIVTVLSLISGCGKTGALGLGHWIDNYA 300
                 +GY+E G +D+A+ LF+AME  GEKPD+VT++SL+SGCG+TGAL LG WID YA
Sbjct: 335 SWTAMIAGYAEKGDLDEAMTLFSAMEAVGEKPDLVTIISLMSGCGQTGALELGKWIDTYA 394

Query: 301 SLHELKKDVVVCNALIDMYAKCGSLNDAREIFYSLPNRTVVSWTAMIAACALNGEFREAL 313
           + + LK +++VCNALID+YAKCGS+++ARE+FY++P +++VSWT +IA CALNGEF+EAL
Sbjct: 395 TANGLKDNLMVCNALIDVYAKCGSMDNARELFYTMPEKSLVSWTTLIAGCALNGEFKEAL 454

BLAST of Cucsa.104830 vs. TAIR10
Match: AT4G19191.1 (AT4G19191.1 Tetratricopeptide repeat (TPR)-like superfamily protein)

HSP 1 Score: 302.8 bits (774), Expect = 3.5e-82
Identity = 167/368 (45.38%), Postives = 223/368 (60.60%), Query Frame = 1

Query: 2   VDMYVKCGKVDDAYNLFDKMPVRNIASWNAMIIGFSQIGSLDRVFNLFMGMRLVGTRPDA 61
           VDM+VKC  VD A  +F++MP R+  +WNAM+ GF Q G  D+ F+LF  MRL    PD+
Sbjct: 94  VDMFVKCNSVDYAAKVFERMPERDATTWNAMLSGFCQSGHTDKAFSLFREMRLNEITPDS 153

Query: 62  ATVIGLTRAVISAKSLRFLKAVHAIGIETGLDADTSVSNTWIAAYSKCGELQLAKMVFHG 121
            TV+ L ++    KSL+ L+A+HA+GI  G+D   +V+NTWI+ Y KCG+L  AK+VF  
Sbjct: 154 VTVMTLIQSASFEKSLKLLEAMHAVGIRLGVDVQVTVANTWISTYGKCGDLDSAKLVFEA 213

Query: 122 IQKTARSSVSWNSLIACYAHFGKYVDAVKSYKGLLCDGFKPD--------ARC------- 181
           I +  R+ VSWNS+   Y+ FG+  DA   Y  +L + FKPD        A C       
Sbjct: 214 IDRGDRTVVSWNSMFKAYSVFGEAFDAFGLYCLMLREEFKPDLSTFINLAASCQNPETLT 273

Query: 182 --------------DSDISLINTLISMYS---------------------------SGYS 241
                         D DI  INT ISMYS                           SGY+
Sbjct: 274 QGRLIHSHAIHLGTDQDIEAINTFISMYSKSEDTCSARLLFDIMTSRTCVSWTVMISGYA 333

Query: 242 EVGRVDDALVLFNAMEETGEKPDIVTVLSLISGCGKTGALGLGHWIDNYASLHELKKD-V 301
           E G +D+AL LF+AM ++GEKPD+VT+LSLISGCGK G+L  G WID  A ++  K+D V
Sbjct: 334 EKGDMDEALALFHAMIKSGEKPDLVTLLSLISGCGKFGSLETGKWIDARADIYGCKRDNV 393

Query: 302 VVCNALIDMYAKCGSLNDAREIFYSLPNRTVVSWTAMIAACALNGEFREALDLFSLLSES 313
           ++CNALIDMY+KCGS+++AR+IF + P +TVV+WT MIA  ALNG F EAL LFS + + 
Sbjct: 394 MICNALIDMYSKCGSIHEARDIFDNTPEKTVVTWTTMIAGYALNGIFLEALKLFSKMIDL 453


HSP 2 Score: 97.8 bits (242), Expect = 1.7e-20
Identity = 85/345 (24.64%), Postives = 154/345 (44.64%), Query Frame = 1

Query: 80  LKAVHAIGIETGLDADTSVSNTWIAAYSKCGELQLAKMV-----FHGIQKTARSSVSWNS 139
           L   HA+ I++G   D     + I+   K G L+  K +      +G ++   + +  N+
Sbjct: 342 LALFHAM-IKSGEKPDLVTLLSLISGCGKFGSLETGKWIDARADIYGCKRD--NVMICNA 401

Query: 140 LIACYAHFGKYVDAVKSYKGLLCDGFKPDARCDSDISLINTLISMYSSGYSEVGRVDDAL 199
           LI  Y+  G   +A   +          D   +  +    T+I+    GY+  G   +AL
Sbjct: 402 LIDMYSKCGSIHEARDIF----------DNTPEKTVVTWTTMIA----GYALNGIFLEAL 461

Query: 200 VLFNAMEETGEKPDIVTVLSLISGCGKTGALGLG-HWIDNYASLHELKKDVVVCNALIDM 259
            LF+ M +   KP+ +T L+++  C  +G+L  G  +      ++ +   +   + ++D+
Sbjct: 462 KLFSKMIDLDYKPNHITFLAVLQACAHSGSLEKGWEYFHIMKQVYNISPGLDHYSCMVDL 521

Query: 260 YAKCGSLNDAREIFYSLPNRTVVS-WTAMIAACALNGEFR----EALDLFSLLSESGIEP 319
             + G L +A E+  ++  +     W A++ AC ++   +     A  LF+L        
Sbjct: 522 LGRKGKLEEALELIRNMSAKPDAGIWGALLNACKIHRNVKIAEQAAESLFNL-------- 581

Query: 320 NNITLTRAYGVHCLVLVRFTTTWKSPRVAVSFVEMANIYASVGRWDEVAAMRKTMRSNQM 379
                                    P++A  +VEMANIYA+ G WD  A +R  M+   +
Sbjct: 582 ------------------------EPQMAAPYVEMANIYAAAGMWDGFARIRSIMKQRNI 637

Query: 380 RKSPGKSVVQVNGMSHVFFVEDRSHHDSLLIYEALGNLAMQMKQK 414
           +K PG+SV+QVNG +H F V +  H ++ +IY  L  L++  K K
Sbjct: 642 KKYPGESVIQVNGKNHSFTVGEHGHVENEVIYFTLNGLSLFAKDK 637


HSP 3 Score: 52.0 bits (123), Expect = 1.1e-06
Identity = 37/149 (24.83%), Postives = 69/149 (46.31%), Query Frame = 1

Query: 1   MVDMYVKCGKVDDAYNLFDKMPVRNIASWNAMIIGFSQIGSLDRVFNLFMGMRLVGTRPD 60
           ++DMY KCG + +A ++FD  P + + +W  MI G++  G       LF  M  +  +P+
Sbjct: 399 LIDMYSKCGSIHEARDIFDNTPEKTVVTWTTMIAGYALNGIFLEALKLFSKMIDLDYKPN 458

Query: 61  AATVIGLTRAVISAKSL-------RFLKAVHAIGIETGLDADTSVSNTWIAAYSKCGELQ 120
             T + + +A   + SL         +K V+   I  GLD  + +    +    + G+L+
Sbjct: 459 HITFLAVLQACAHSGSLEKGWEYFHIMKQVY--NISPGLDHYSCM----VDLLGRKGKLE 518

Query: 121 LAKMVFHGIQKTARSSVSWNSLI-ACYAH 142
            A  +   +     + + W +L+ AC  H
Sbjct: 519 EALELIRNMSAKPDAGI-WGALLNACKIH 540

BLAST of Cucsa.104830 vs. TAIR10
Match: AT2G22410.1 (AT2G22410.1 SLOW GROWTH 1)

HSP 1 Score: 181.4 bits (459), Expect = 1.2e-45
Identity = 99/320 (30.94%), Postives = 170/320 (53.12%), Query Frame = 1

Query: 2   VDMYVKCGKVDDAYNLFDKMPVRNIASWNAMIIGFSQIGSLDRVFNLFMGMRLVGTRPDA 61
           + M+  CG +++A  +FD+ PVR++ SWN +I G+ +IG  ++   ++  M   G +PD 
Sbjct: 198 IHMFASCGDMENARKVFDESPVRDLVSWNCLINGYKKIGEAEKAIYVYKLMESEGVKPDD 257

Query: 62  ATVIGLTRAVISAKSLRFLKAVHAIGIETGLDADTSVSNTWIAAYSKCGELQLAKMVFHG 121
            T+IGL  +      L   K  +    E GL     + N  +  +SKCG++  A+ +F  
Sbjct: 258 VTMIGLVSSCSMLGDLNRGKEFYEYVKENGLRMTIPLVNALMDMFSKCGDIHEARRIFDN 317

Query: 122 IQKTARSSVSWNSLIACYAHFGKYVDAVKSYKGLLCDGFKPDARCDSDISLINTLISMYS 181
           ++K  R+ VSW ++I+ YA  G    + K +          D   + D+ L N +I    
Sbjct: 318 LEK--RTIVSWTTMISGYARCGLLDVSRKLF----------DDMEEKDVVLWNAMIG--- 377

Query: 182 SGYSEVGRVDDALVLFNAMEETGEKPDIVTVLSLISGCGKTGALGLGHWIDNYASLHELK 241
            G  +  R  DAL LF  M+ +  KPD +T++  +S C + GAL +G WI  Y   + L 
Sbjct: 378 -GSVQAKRGQDALALFQEMQTSNTKPDEITMIHCLSACSQLGALDVGIWIHRYIEKYSLS 437

Query: 242 KDVVVCNALIDMYAKCGSLNDAREIFYSLPNRTVVSWTAMIAACALNGEFREALDLFSLL 301
            +V +  +L+DMYAKCG++++A  +F+ +  R  +++TA+I   AL+G+   A+  F+ +
Sbjct: 438 LNVALGTSLVDMYAKCGNISEALSVFHGIQTRNSLTYTAIIGGLALHGDASTAISYFNEM 497

Query: 302 SESGIEPNNITLTRAYGVHC 322
            ++GI P+ IT        C
Sbjct: 498 IDAGIAPDEITFIGLLSACC 501


HSP 2 Score: 105.1 bits (261), Expect = 1.1e-22
Identity = 98/415 (23.61%), Postives = 164/415 (39.52%), Query Frame = 1

Query: 1   MVDMYVKCGKVDDAYNLFDKMPVRNIASWNAMIIGFSQIGSLDRVFNLFMGMRLVGTRPD 60
           ++DM+ KCG + +A  +FD +  R I SW  MI G+++ G LD    LF  M        
Sbjct: 298 LMDMFSKCGDIHEARRIFDNLEKRTIVSWTTMISGYARCGLLDVSRKLFDDMEEKDVVLW 357

Query: 61  AATVIGLTRAVISAKSLRFLKAVHAIGIETGLDADTSVSNTWIAAYSKCGELQLAKMVFH 120
            A + G  +A     +L   + +      +    D       ++A S+ G L +   +  
Sbjct: 358 NAMIGGSVQAKRGQDALALFQEMQT----SNTKPDEITMIHCLSACSQLGALDVGIWIHR 417

Query: 121 GIQKTARS--SVSWNSLIACYAHFGKYVDAVKSYKGLLCDGFKPDARCDSDISLINTLIS 180
            I+K + S       SL+  YA  G   +A+  + G+            +  SL  T I 
Sbjct: 418 YIEKYSLSLNVALGTSLVDMYAKCGNISEALSVFHGI-----------QTRNSLTYTAI- 477

Query: 181 MYSSGYSEVGRVDDALVLFNAMEETGEKPDIVTVLSLISGCGKTGALGLGH-WIDNYASL 240
               G +  G    A+  FN M + G  PD +T + L+S C   G +  G  +     S 
Sbjct: 478 --IGGLALHGDASTAISYFNEMIDAGIAPDEITFIGLLSACCHGGMIQTGRDYFSQMKSR 537

Query: 241 HELKKDVVVCNALIDMYAKCGSLNDAREIFYSLP-NRTVVSWTAMIAACALNGEFREALD 300
             L   +   + ++D+  + G L +A  +  S+P       W A++  C ++G       
Sbjct: 538 FNLNPQLKHYSIMVDLLGRAGLLEEADRLMESMPMEADAAVWGALLFGCRMHG------- 597

Query: 301 LFSLLSESGIEPNNITLTRAYGVHCLVLVRFTTTWKSPRVAVSFVEMANIYASVGRWDEV 360
                        N+ L        L L         P  +  +V +  +Y     W++ 
Sbjct: 598 -------------NVELGEKAAKKLLEL--------DPSDSGIYVLLDGMYGEANMWEDA 657

Query: 361 AAMRKTMRSNQMRKSPGKSVVQVNGMSHVFFVEDRSHHDSLLIYEALGNLAMQMK 412
              R+ M    + K PG S ++VNG+   F V D+S  +S  IY+ L  L   M+
Sbjct: 658 KRARRMMNERGVEKIPGCSSIEVNGIVCEFIVRDKSRPESEKIYDRLHCLGRHMR 666


HSP 3 Score: 102.4 bits (254), Expect = 6.9e-22
Identity = 89/304 (29.28%), Postives = 142/304 (46.71%), Query Frame = 1

Query: 25  NIASWNAMIIGFSQIGSLDRVFNLFMGMRLVG---TRPDAAT--VIGLTRAVISAKSLRF 84
           NI SWN  I GFS+  +    F L+  M   G   +RPD  T  V+    A +   SL  
Sbjct: 117 NIFSWNVTIRGFSESENPKESFLLYKQMLRHGCCESRPDHFTYPVLFKVCADLRLSSLGH 176

Query: 85  LKAVHAIGIETGLDADTSVSNTWIAAYSKCGELQLAKMVFHGIQKTARSSVSWNSLIACY 144
           +   H + +   L+  + V N  I  ++ CG+++ A+ VF   +   R  VSWN LI  Y
Sbjct: 177 MILGHVLKLR--LELVSHVHNASIHMFASCGDMENARKVFD--ESPVRDLVSWNCLINGY 236

Query: 145 AHFGKYVDAVKSYKGLLCDGFKPDARCDSDISLINTLISMYSSGYSEVGRVDDALVLFNA 204
              G+   A+  YK +  +G KPD     D+++I  +     S  S +G ++     +  
Sbjct: 237 KKIGEAEKAIYVYKLMESEGVKPD-----DVTMIGLV-----SSCSMLGDLNRGKEFYEY 296

Query: 205 MEETGEKPDIVTVLSLISGCGKTGALGLGHWI-DNYASLHELKKDVVVCNALIDMYAKCG 264
           ++E G +  I  V +L+    K G +     I DN       K+ +V    +I  YA+CG
Sbjct: 297 VKENGLRMTIPLVNALMDMFSKCGDIHEARRIFDNLE-----KRTIVSWTTMISGYARCG 356

Query: 265 SLNDAREIFYSLPNRTVVSWTAMIAACALNGEFREALDLFSLLSESGIEPNNITLTRAYG 323
            L+ +R++F  +  + VV W AMI         ++AL LF  +  S  +P+ IT+     
Sbjct: 357 LLDVSRKLFDDMEEKDVVLWNAMIGGSVQAKRGQDALALFQEMQTSNTKPDEITM----- 396

BLAST of Cucsa.104830 vs. TAIR10
Match: AT2G29760.1 (AT2G29760.1 Tetratricopeptide repeat (TPR)-like superfamily protein)

HSP 1 Score: 175.6 bits (444), Expect = 6.4e-44
Identity = 97/318 (30.50%), Postives = 172/318 (54.09%), Query Frame = 1

Query: 1   MVDMYVKCGKVDDAYNLFDKMPVRNIASWNAMIIGFSQIGSLDRVFNLFMGMRLVGTRPD 60
           ++  Y  CG +D A  +F  +  +++ SWN+MI GF Q GS D+   LF  M     +  
Sbjct: 172 LIHCYFSCGDLDSACKVFTTIKEKDVVSWNSMINGFVQKGSPDKALELFKKMESEDVKAS 231

Query: 61  AATVIGLTRAVISAKSLRFLKAVHAIGIETGLDADTSVSNTWIAAYSKCGELQLAKMVFH 120
             T++G+  A    ++L F + V +   E  ++ + +++N  +  Y+KCG ++ AK +F 
Sbjct: 232 HVTMVGVLSACAKIRNLEFGRQVCSYIEENRVNVNLTLANAMLDMYTKCGSIEDAKRLFD 291

Query: 121 GIQKTARSSVSWNSLIACYAHFGKYVDAVKSYKGLLCDGFKPDARCDSDISLINTLISMY 180
            +++  + +V+W +++  YA    Y  A +    +             DI   N LIS Y
Sbjct: 292 AMEE--KDNVTWTTMLDGYAISEDYEAAREVLNSMP----------QKDIVAWNALISAY 351

Query: 181 SSGYSEVGRVDDALVLFNAME-ETGEKPDIVTVLSLISGCGKTGALGLGHWIDNYASLHE 240
                  G+ ++AL++F+ ++ +   K + +T++S +S C + GAL LG WI +Y   H 
Sbjct: 352 EQN----GKPNEALIVFHELQLQKNMKLNQITLVSTLSACAQVGALELGRWIHSYIKKHG 411

Query: 241 LKKDVVVCNALIDMYAKCGSLNDAREIFYSLPNRTVVSWTAMIAACALNGEFREALDLFS 300
           ++ +  V +ALI MY+KCG L  +RE+F S+  R V  W+AMI   A++G   EA+D+F 
Sbjct: 412 IRMNFHVTSALIHMYSKCGDLEKSREVFNSVEKRDVFVWSAMIGGLAMHGCGNEAVDMFY 471

Query: 301 LLSESGIEPNNITLTRAY 318
            + E+ ++PN +T T  +
Sbjct: 472 KMQEANVKPNGVTFTNVF 473


HSP 2 Score: 104.0 bits (258), Expect = 2.4e-22
Identity = 103/441 (23.36%), Postives = 189/441 (42.86%), Query Frame = 1

Query: 1   MVDMYVKCGKVDDAYNLFDKMPVRNIASWNAMIIGFSQIGSLDRVFNLFMGMRLVGTRPD 60
           M+DMY KCG ++DA  LFD M  ++  +W  M+ G++     +    +   M      P 
Sbjct: 273 MLDMYTKCGSIEDAKRLFDAMEEKDNVTWTTMLDGYAISEDYEAAREVLNSM------PQ 332

Query: 61  AATVIGLTRAVISA-----KSLRFLKAVHAIGIETGLDADTSVSNTWIAAYSKCGELQLA 120
              V     A+ISA     K    L   H + ++  +  +     + ++A ++ G L+L 
Sbjct: 333 KDIVAW--NALISAYEQNGKPNEALIVFHELQLQKNMKLNQITLVSTLSACAQVGALELG 392

Query: 121 KMVF-----HGIQKTARSSVSWNSLIACYAHFGKYVDAVKSYKGLLCDGFKPDARCDSDI 180
           + +      HGI+     +   ++LI  Y+  G    + + +  +             D+
Sbjct: 393 RWIHSYIKKHGIRMNFHVT---SALIHMYSKCGDLEKSREVFNSVE----------KRDV 452

Query: 181 SLINTLISMYSSGYSEVGRVDDALVLFNAMEETGEKPDIVTVLSLISGCGKTG----ALG 240
            + + +I     G +  G  ++A+ +F  M+E   KP+ VT  ++   C  TG    A  
Sbjct: 453 FVWSAMIG----GLAMHGCGNEAVDMFYKMQEANVKPNGVTFTNVFCACSHTGLVDEAES 512

Query: 241 LGHWID-NYASLHELKKDVVVCNALIDMYAKCGSLNDAREIFYSLPNRTVVS-WTAMIAA 300
           L H ++ NY  + E K    +    +D+  + G L  A +   ++P     S W A++ A
Sbjct: 513 LFHQMESNYGIVPEEKHYACI----VDVLGRSGYLEKAVKFIEAMPIPPSTSVWGALLGA 572

Query: 301 CALNGEFREALDLFSLLSESGIEPNNITLTRAYGVHCLVLVRFTTTWKSPRVAVSFVEMA 360
           C ++     A    + L E  +EP N       G H L                    ++
Sbjct: 573 CKIHANLNLAEMACTRLLE--LEPRND------GAHVL--------------------LS 632

Query: 361 NIYASVGRWDEVAAMRKTMRSNQMRKSPGKSVVQVNGMSHVFFVEDRSHHDSLLIYEALG 420
           NIYA +G+W+ V+ +RK MR   ++K PG S ++++GM H F   D +H  S  +Y  L 
Sbjct: 633 NIYAKLGKWENVSELRKHMRVTGLKKEPGCSSIEIDGMIHEFLSGDNAHPMSEKVYGKLH 656

Query: 421 NLAMQMKQKEFSSHAQRWVEL 426
            +  ++K   +     + +++
Sbjct: 693 EVMEKLKSNGYEPEISQVLQI 656


HSP 3 Score: 93.6 bits (231), Expect = 3.2e-19
Identity = 83/316 (26.27%), Postives = 145/316 (45.89%), Query Frame = 1

Query: 14  AYNLFDKMPVRNIASWNAMIIGFSQIGSLDRVFNLFMGMRLVGTR---PDAATVIGLTRA 73
           A  +FD++P  N  +WN +I  ++     D V +++  + +V      P+  T   L +A
Sbjct: 83  ARKVFDEIPKPNSFAWNTLIRAYAS--GPDPVLSIWAFLDMVSESQCYPNKYTFPFLIKA 142

Query: 74  VISAKSLRFLKAVHAIGIETGLDADTSVSNTWIAAYSKCGELQLAKMVFHGIQKTARSSV 133
                SL   +++H + +++ + +D  V+N+ I  Y  CG+L  A  VF  I++  +  V
Sbjct: 143 AAEVSSLSLGQSLHGMAVKSAVGSDVFVANSLIHCYFSCGDLDSACKVFTTIKE--KDVV 202

Query: 134 SWNSLIACYAHFGKYVDAVKSYKGLLCDGFKPDARCDSDISLINTLISMYS--------- 193
           SWNS+I  +   G    A++ +K +  +  K      S ++++  L +            
Sbjct: 203 SWNSMINGFVQKGSPDKALELFKKMESEDVKA-----SHVTMVGVLSACAKIRNLEFGRQ 262

Query: 194 -SGYSEVGRVDDALVLFNAMEETGEKPDIVTVLSLISGCGKTGALGLGHWIDNYASLHEL 253
              Y E  RV+  L L NAM            L + + CG          I++   L + 
Sbjct: 263 VCSYIEENRVNVNLTLANAM------------LDMYTKCGS---------IEDAKRLFDA 322

Query: 254 --KKDVVVCNALIDMYAKCGSLNDAREIFYSLPNRTVVSWTAMIAACALNGEFREALDLF 313
             +KD V    ++D YA       ARE+  S+P + +V+W A+I+A   NG+  EAL +F
Sbjct: 323 MEEKDNVTWTTMLDGYAISEDYEAAREVLNSMPQKDIVAWNALISAYEQNGKPNEALIVF 368

BLAST of Cucsa.104830 vs. TAIR10
Match: AT4G14820.1 (AT4G14820.1 Pentatricopeptide repeat (PPR) superfamily protein)

HSP 1 Score: 171.0 bits (432), Expect = 1.6e-42
Identity = 96/312 (30.77%), Postives = 168/312 (53.85%), Query Frame = 1

Query: 2   VDMYVKCGKVDDAYNLFDKMPVRNIASWNAMIIGFSQIGSLDRVFNLFMGMRLVGTRPDA 61
           +DMY  CG+++ A N+FD+M  R++ +WN MI  + + G +D  F LF  M+     PD 
Sbjct: 153 MDMYASCGRINYARNVFDEMSHRDVVTWNTMIERYCRFGLVDEAFKLFEEMKDSNVMPDE 212

Query: 62  ATVIGLTRAVISAKSLRFLKAVHAIGIETGLDADTSVSNTWIAAYSKCGELQLAKMVFHG 121
             +  +  A     ++R+ +A++   IE  +  DT +    +  Y+  G + +A+  F  
Sbjct: 213 MILCNIVSACGRTGNMRYNRAIYEFLIENDVRMDTHLLTALVTMYAGAGCMDMAREFFR- 272

Query: 122 IQKTARSSVSWNSLIACYAHFGKYVDAVKSYKGLLCDGFKPDARCDSDISLINTLISMY- 181
            + + R+     ++++ Y+  G+  DA   +          D     D+    T+IS Y 
Sbjct: 273 -KMSVRNLFVSTAMVSGYSKCGRLDDAQVIF----------DQTEKKDLVCWTTMISAYV 332

Query: 182 SSGYSEVGRVDDALVLFNAMEETGEKPDIVTVLSLISGCGKTGALGLGHWIDNYASLHEL 241
            S Y +     +AL +F  M  +G KPD+V++ S+IS C   G L    W+ +   ++ L
Sbjct: 333 ESDYPQ-----EALRVFEEMCCSGIKPDVVSMFSVISACANLGILDKAKWVHSCIHVNGL 392

Query: 242 KKDVVVCNALIDMYAKCGSLNDAREIFYSLPNRTVVSWTAMIAACALNGEFREALDLFSL 301
           + ++ + NALI+MYAKCG L+  R++F  +P R VVSW++MI A +++GE  +AL LF+ 
Sbjct: 393 ESELSINNALINMYAKCGGLDATRDVFEKMPRRNVVSWSSMINALSMHGEASDALSLFAR 447

Query: 302 LSESGIEPNNIT 313
           + +  +EPN +T
Sbjct: 453 MKQENVEPNEVT 447


HSP 2 Score: 112.8 bits (281), Expect = 5.1e-25
Identity = 96/412 (23.30%), Postives = 172/412 (41.75%), Query Frame = 1

Query: 1   MVDMYVKCGKVDDAYNLFDKMPVRNIASWNAMIIGFSQIGSLDRVFNLFMGMRLVGTRPD 60
           MV  Y KCG++DDA  +FD+   +++  W  MI  + +         +F  M   G +PD
Sbjct: 284 MVSGYSKCGRLDDAQVIFDQTEKKDLVCWTTMISAYVESDYPQEALRVFEEMCCSGIKPD 343

Query: 61  AATVIGLTRAVISAKSLRFLKAVHAIGIETGLDADTSVSNTWIAAYSKCGELQLAKMVFH 120
             ++  +  A  +   L   K VH+     GL+++ S++N  I  Y+KCG L   + VF 
Sbjct: 344 VVSMFSVISACANLGILDKAKWVHSCIHVNGLESELSINNALINMYAKCGGLDATRDVFE 403

Query: 121 GIQKTARSSVSWNSLIACYAHFGKYVDAVKSYKGLLCDGFKPDARCDSDISLINTLISMY 180
            + +  R+ VSW+S+I   +  G+  DA+  +  +  +  +P     ++++ +  L    
Sbjct: 404 KMPR--RNVVSWSSMINALSMHGEASDALSLFARMKQENVEP-----NEVTFVGVLY--- 463

Query: 181 SSGYSEVGRVDDALVLFNAM-EETGEKPDIVTVLSLISGCGKTGALGLGHWIDNYASLHE 240
             G S  G V++   +F +M +E    P +     ++   G+   L          +L  
Sbjct: 464 --GCSHSGLVEEGKKIFASMTDEYNITPKLEHYGCMVDLFGRANLL--------REALEV 523

Query: 241 LKKDVVVCNALIDMYAKCGSLNDAREIFYSLPNRTVVSWTAMIAACALNGEFREALDLFS 300
           ++   V  N +I                          W ++++AC ++GE    L  F+
Sbjct: 524 IESMPVASNVVI--------------------------WGSLMSACRIHGELE--LGKFA 583

Query: 301 LLSESGIEPNNITLTRAYGVHCLVLVRFTTTWKSPRVAVSFVEMANIYASVGRWDEVAAM 360
                 +EP++                            + V M+NIYA   RW++V  +
Sbjct: 584 AKRILELEPDH--------------------------DGALVLMSNIYAREQRWEDVRNI 621

Query: 361 RKTMRSNQMRKSPGKSVVQVNGMSHVFFVEDRSHHDSLLIYEALGNLAMQMK 412
           R+ M    + K  G S +  NG SH F + D+ H  S  IY  L  +  ++K
Sbjct: 644 RRVMEEKNVFKEKGLSRIDQNGKSHEFLIGDKRHKQSNEIYAKLDEVVSKLK 621


HSP 3 Score: 87.8 bits (216), Expect = 1.8e-17
Identity = 83/378 (21.96%), Postives = 156/378 (41.27%), Query Frame = 1

Query: 14  AYNLFDKMPVR-NIASWNAMIIGFSQIGSLDRVFNLFMGMRLVGTRPDAATVIGLTRAVI 73
           A N+F  +P       +N  +   S+          +  +R VG R D  + + + +AV 
Sbjct: 63  ALNVFSSIPSPPESIVFNPFLRDLSRSSEPRATILFYQRIRHVGGRLDQFSFLPILKAVS 122

Query: 74  SAKSLRFLKAVHAIGIETGLDADTSVSNTWIAAYSKCGELQLAKMVFHGIQKTARSSVSW 133
              +L     +H +  +     D  V   ++  Y+ CG +  A+ VF   + + R  V+W
Sbjct: 123 KVSALFEGMELHGVAFKIATLCDPFVETGFMDMYASCGRINYARNVFD--EMSHRDVVTW 182

Query: 134 NSLIACYAHFGKYVDAVKSYKGLLCDGFKPDARCDSDISLINTLISMYSSGYSEVGRVDD 193
           N++I  Y  FG   +A K ++ +      PD     ++ L N + +   +G     R   
Sbjct: 183 NTMIERYCRFGLVDEAFKLFEEMKDSNVMPD-----EMILCNIVSACGRTGNMRYNRA-- 242

Query: 194 ALVLFNAMEETGEKPDIVTVLSLISGCGKTGALGLGHWIDNYASLHELKKDVVVCNALID 253
              ++  + E   + D   + +L++     G + +        S+  L     V  A++ 
Sbjct: 243 ---IYEFLIENDVRMDTHLLTALVTMYAGAGCMDMAREFFRKMSVRNL----FVSTAMVS 302

Query: 254 MYAKCGSLNDAREIFYSLPNRTVVSWTAMIAACALNGEFREALDLFSLLSESGIEPNNIT 313
            Y+KCG L+DA+ IF     + +V WT MI+A   +   +EAL +F  +  SGI+P+ ++
Sbjct: 303 GYSKCGRLDDAQVIFDQTEKKDLVCWTTMISAYVESDYPQEALRVFEEMCCSGIKPDVVS 362

Query: 314 LTRAYGVHCLVLVRFTTTWKSPRVAVSFVE--------MANIYASVGRWDEVAAMRKTMR 373
           +         + +     W    + V+ +E        + N+YA  G  D          
Sbjct: 363 MFSVISACANLGILDKAKWVHSCIHVNGLESELSINNALINMYAKCGGLDAT-------- 416

Query: 374 SNQMRKSPGKSVVQVNGM 383
            +   K P ++VV  + M
Sbjct: 423 RDVFEKMPRRNVVSWSSM 416

BLAST of Cucsa.104830 vs. TAIR10
Match: AT3G15930.1 (AT3G15930.1 Pentatricopeptide repeat (PPR) superfamily protein)

HSP 1 Score: 164.1 bits (414), Expect = 1.9e-40
Identity = 96/314 (30.57%), Postives = 158/314 (50.32%), Query Frame = 1

Query: 1   MVDMYVKCGKVDDAYNLFDKMPVRNIASWNAMIIGFSQIGSLDRVFNLFMGMRLVGTRPD 60
           +V MY  CG +D A  +FD+    ++ SWN MI G++++   +    L + M      P 
Sbjct: 176 LVKMYSLCGLMDMARGVFDRRCKEDVFSWNLMISGYNRMKEYEESIELLVEMERNLVSPT 235

Query: 61  AATVIGLTRAVISAKSLRFLKAVHAIGIETGLDADTSVSNTWIAAYSKCGELQLAKMVFH 120
           + T++ +  A    K     K VH    E   +    + N  + AY+ CGE+ +A  +F 
Sbjct: 236 SVTLLLVLSACSKVKDKDLCKRVHEYVSECKTEPSLRLENALVNAYAACGEMDIAVRIFR 295

Query: 121 GIQKTARSSVSWNSLIACYAHFGKYVDAVKSYKGLLCDGFKPDARCDSDISLINTLIS-- 180
            ++  AR  +SW S++                KG +  G    AR   D   +   IS  
Sbjct: 296 SMK--ARDVISWTSIV----------------KGYVERGNLKLARTYFDQMPVRDRISWT 355

Query: 181 MYSSGYSEVGRVDDALVLFNAMEETGEKPDIVTVLSLISGCGKTGALGLGHWIDNYASLH 240
           +   GY   G  +++L +F  M+  G  PD  T++S+++ C   G+L +G WI  Y   +
Sbjct: 356 IMIDGYLRAGCFNESLEIFREMQSAGMIPDEFTMVSVLTACAHLGSLEIGEWIKTYIDKN 415

Query: 241 ELKKDVVVCNALIDMYAKCGSLNDAREIFYSLPNRTVVSWTAMIAACALNGEFREALDLF 300
           ++K DVVV NALIDMY KCG    A+++F+ +  R   +WTAM+   A NG+ +EA+ +F
Sbjct: 416 KIKNDVVVGNALIDMYFKCGCSEKAQKVFHDMDQRDKFTWTAMVVGLANNGQGQEAIKVF 471

Query: 301 SLLSESGIEPNNIT 313
             + +  I+P++IT
Sbjct: 476 FQMQDMSIQPDDIT 471


HSP 2 Score: 107.8 bits (268), Expect = 1.6e-23
Identity = 101/413 (24.46%), Postives = 179/413 (43.34%), Query Frame = 1

Query: 1   MVDMYVKCGKVDDAYNLFDKMPVRNIASWNAMIIGFSQIGSLDRVFNLFMGMRLVGTRPD 60
           +V+ Y  CG++D A  +F  M  R++ SW +++ G+ + G+L      F  M +      
Sbjct: 277 LVNAYAACGEMDIAVRIFRSMKARDVISWTSIVKGYVERGNLKLARTYFDQMPVRDRISW 336

Query: 61  AATVIGLTRAVISAKSLRFLKAVHAIGIETGLDADTSVSNTWIAAYSKCGELQLAKMVFH 120
              + G  RA    +SL   + + + G+    D  T VS   + A +  G L++ + +  
Sbjct: 337 TIMIDGYLRAGCFNESLEIFREMQSAGMIP--DEFTMVSV--LTACAHLGSLEIGEWIKT 396

Query: 121 GIQKTARSS--VSWNSLIACYAHFGKYVDAVKSYKGLLCDGFKPDARCDSDISLINTLIS 180
            I K    +  V  N+LI  Y   G    A K +              D D     T  +
Sbjct: 397 YIDKNKIKNDVVVGNALIDMYFKCGCSEKAQKVFH-------------DMDQRDKFTWTA 456

Query: 181 MYSSGYSEVGRVDDALVLFNAMEETGEKPDIVTVLSLISGCGKTGALGLGH-WIDNYASL 240
           M   G +  G+  +A+ +F  M++   +PD +T L ++S C  +G +     +     S 
Sbjct: 457 MVV-GLANNGQGQEAIKVFFQMQDMSIQPDDITYLGVLSACNHSGMVDQARKFFAKMRSD 516

Query: 241 HELKKDVVVCNALIDMYAKCGSLNDAREIFYSLP-NRTVVSWTAMIAACALNGEFREALD 300
           H ++  +V    ++DM  + G + +A EI   +P N   + W A++ A  L+ +  E + 
Sbjct: 517 HRIEPSLVHYGCMVDMLGRAGLVKEAYEILRKMPMNPNSIVWGALLGASRLHND--EPMA 576

Query: 301 LFSLLSESGIEPNNITLTRAYGVHCLVLVRFTTTWKSPRVAVSFVEMANIYASVGRWDEV 360
             +      +EP+N  +   Y + C                       NIYA   RW ++
Sbjct: 577 ELAAKKILELEPDNGAV---YALLC-----------------------NIYAGCKRWKDL 636

Query: 361 AAMRKTMRSNQMRKSPGKSVVQVNGMSHVFFVEDRSHHDSLLIYEALGNLAMQ 410
             +R+ +    ++K+PG S+++VNG +H F   D+SH  S  IY  L  LA +
Sbjct: 637 REVRRKIVDVAIKKTPGFSLIEVNGFAHEFVAGDKSHLQSEEIYMKLEELAQE 643


HSP 3 Score: 58.2 bits (139), Expect = 1.5e-08
Identity = 41/148 (27.70%), Postives = 73/148 (49.32%), Query Frame = 1

Query: 167 DSDISLINTLISMYSSGYSEVGRVDDALVLFNAMEETGEKPDIVTVLSLISGCGKTG-AL 226
           + D+ + N +I     G+S+V    + + L+  M + G  PD  T   L++G  + G AL
Sbjct: 96  EPDVVVWNNMIK----GWSKVDCDGEGVRLYLNMLKEGVTPDSHTFPFLLNGLKRDGGAL 155

Query: 227 GLGHWIDNYASLHELKKDVVVCNALIDMYAKCGSLNDAREIFYSLPNRTVVSWTAMIAAC 286
             G  +  +     L  ++ V NAL+ MY+ CG ++ AR +F       V SW  MI+  
Sbjct: 156 ACGKKLHCHVVKFGLGSNLYVQNALVKMYSLCGLMDMARGVFDRRCKEDVFSWNLMISGY 215

Query: 287 ALNGEFREALDLFSLLSESGIEPNNITL 314
               E+ E+++L   +  + + P ++TL
Sbjct: 216 NRMKEYEESIELLVEMERNLVSPTSVTL 239

BLAST of Cucsa.104830 vs. NCBI nr
Match: gi|449451649|ref|XP_004143574.1| (PREDICTED: pentatricopeptide repeat-containing protein At4g19191, mitochondrial [Cucumis sativus])

HSP 1 Score: 468.8 bits (1205), Expect = 1.0e-128
Identity = 261/381 (68.50%), Postives = 275/381 (72.18%), Query Frame = 1

Query: 1   MVDMYVKCGKVDDAYNLFDKMPVRNIASWNAMIIGFSQIGSLDRVFNLFMGMRLVGTRPD 60
           MVDMYVKCGKVDDAYNLFDKMPVRNIASWNAMIIGFSQIGSLDRVFNLFMGMRLVGTRPD
Sbjct: 93  MVDMYVKCGKVDDAYNLFDKMPVRNIASWNAMIIGFSQIGSLDRVFNLFMGMRLVGTRPD 152

Query: 61  AATVIGLTRAVISAKSLRFLKAVHAIGIETGLDADTSVSNTWIAAYSKCGELQLAKMVFH 120
           AATVIGLTRAVISAKSLRFLKAVHAIGIETGLDADTSVSNTWIAAYSKCGELQLAKMVFH
Sbjct: 153 AATVIGLTRAVISAKSLRFLKAVHAIGIETGLDADTSVSNTWIAAYSKCGELQLAKMVFH 212

Query: 121 GIQKTARSSVSWNSLIACYAHFGKYVDAVKSYKGLLCDGFKPDAR--------------- 180
           GIQKTARSSVSWNSLIACYAHFGKYVDAVKSYKGLLCDGFKPDA                
Sbjct: 213 GIQKTARSSVSWNSLIACYAHFGKYVDAVKSYKGLLCDGFKPDASTIISLLSSCQQPEAL 272

Query: 181 --------------CDSDISLINTLISMYSSGYSEVGRVDDALVLFNAM----------- 240
                         CDSDISLINTLISM    YS  G +  A +LF+ M           
Sbjct: 273 IYGFLIHGHGFQLGCDSDISLINTLISM----YSRCGDISSATILFDGMSIRTCVSWTAM 332

Query: 241 ----EETGEKPDIVTVLSLISGCGKTGAL--------GLGH----WIDNYASLHELKKD- 300
                E G   D + + + +   G+   +        G G      + ++   +    + 
Sbjct: 333 ISGYSEVGRVDDALVLFNAMEETGEKPDIVTVLSLISGCGKTGALGLGHWIDNYASLHEL 392

Query: 301 ---VVVCNALIDMYAKCGSLNDAREIFYSLPNRTVVSWTAMIAACALNGEFREALDLFSL 322
              VVVCNALIDMYAKCGSLNDAREIFYSLPNRTVVSWTAMIAACALNGEFREALDLFSL
Sbjct: 393 KKDVVVCNALIDMYAKCGSLNDAREIFYSLPNRTVVSWTAMIAACALNGEFREALDLFSL 452

BLAST of Cucsa.104830 vs. NCBI nr
Match: gi|449451649|ref|XP_004143574.1| (PREDICTED: pentatricopeptide repeat-containing protein At4g19191, mitochondrial [Cucumis sativus])

HSP 1 Score: 232.6 bits (592), Expect = 1.3e-57
Identity = 165/432 (38.19%), Postives = 214/432 (49.54%), Query Frame = 1

Query: 1   MVDMYVKCGKVDDAYNLFDKMPVRNIASWNAMIIGFSQIGSLDRVFNLFMGMRLVGTRPD 60
           ++ MY +CG +  A  LFD M +R   SW AMI                 G   VG   D
Sbjct: 297 LISMYSRCGDISSATILFDGMSIRTCVSWTAMI----------------SGYSEVGRVDD 356

Query: 61  AATVIGLTRAVISAKSLRFLKAVHAIGIETGLDADTSVSNTWIAAYSKCGELQLAKMV-- 120
           A  +                        ETG   D     + I+   K G L L   +  
Sbjct: 357 ALVLFNAME-------------------ETGEKPDIVTVLSLISGCGKTGALGLGHWIDN 416

Query: 121 FHGIQKTARSSVSWNSLIACYAHFGKYVDAVKSYKGLLCDGFKPDARCDSDISLINTLIS 180
           +  + +  +  V  N+LI  YA  G   DA + +  L      P+    S  ++I     
Sbjct: 417 YASLHELKKDVVVCNALIDMYAKCGSLNDAREIFYSL------PNRTVVSWTAMI----- 476

Query: 181 MYSSGYSEVGRVDDALVLFNAMEETGEKPDIVTVLSLISGCGKTGALGLGH-WIDNYASL 240
              +  +  G   +AL LF+ + E+G +P+ +T L+++  C   G L  G          
Sbjct: 477 ---AACALNGEFREALDLFSLLSESGIEPNNITFLAVLQACCHGGYLEKGRECFMMMTER 536

Query: 241 HELKKDVVVCNALIDMYAKCGSLNDAREIFYSLPNRTVVS-WTAMIAACALNGEFREALD 300
           + +   +   + +ID+  + G L +A E+   +P +     W A++ AC ++        
Sbjct: 537 YGINPGLDHYSCMIDLLGRKGKLIEALEVIQDMPMKPDEGIWGALLGACKIH-------- 596

Query: 301 LFSLLSESGIEPNNITLTRAYGVHCLVLVRFTTTWKSPRVAVSFVEMANIYASVGRWDEV 360
                       NN+ +      +   L         PRVAVSFVEMANIYASVGRWDEV
Sbjct: 597 ------------NNMEIGEYVSRYLFEL--------QPRVAVSFVEMANIYASVGRWDEV 651

Query: 361 AAMRKTMRSNQMRKSPGKSVVQVNGMSHVFFVEDRSHHDSLLIYEALGNLAMQMKQKEFS 420
           AAMRKTMRSNQMRKSPGKSVVQVNGMSHVFFVEDRSHHDSLLIYEALGNLAMQMKQKEFS
Sbjct: 657 AAMRKTMRSNQMRKSPGKSVVQVNGMSHVFFVEDRSHHDSLLIYEALGNLAMQMKQKEFS 651

Query: 421 SHAQRWVELDTI 429
           SHAQRWVELDTI
Sbjct: 717 SHAQRWVELDTI 651


HSP 2 Score: 69.7 bits (169), Expect = 1.4e-08
Identity = 48/151 (31.79%), Postives = 68/151 (45.03%), Query Frame = 1

Query: 171 SLINTLISMYSS--GYSEVGRVDDALVLFNAMEETGEKPDIVTVLSLISGCGKTGALGLG 230
           S ++TL    SS  G    G    AL LF+ ++  G +P+  T   L   C K   L   
Sbjct: 12  SKLSTLTWWNSSIRGAVNQGNASKALALFHQLKLNGLQPNNFTFPFLSKACAKLSHLTNS 71

Query: 231 HWIDNYASLHELKKDVVVCNALIDMYAKCGSLNDAREIFYSLPNRTVVSWTAMIAACALN 290
             I  +        D+ V  A++DMY KCG ++DA  +F  +P R + SW AMI   +  
Sbjct: 72  QIIHTHVVKSPFYSDIYVQTAMVDMYVKCGKVDDAYNLFDKMPVRNIASWNAMIIGFSQI 131

Query: 291 GEFREALDLFSLLSESGIEPNNIT---LTRA 317
           G      +LF  +   G  P+  T   LTRA
Sbjct: 132 GSLDRVFNLFMGMRLVGTRPDAATVIGLTRA 162


HSP 3 Score: 452.6 bits (1163), Expect = 7.7e-124
Identity = 253/381 (66.40%), Postives = 268/381 (70.34%), Query Frame = 1

Query: 1   MVDMYVKCGKVDDAYNLFDKMPVRNIASWNAMIIGFSQIGSLDRVFNLFMGMRLVGTRPD 60
           MVDMYVKCGK +DAYNLFDKMPVRNIASWNAMI+GFSQIGSLDRVFNLFMGMRLVGTRPD
Sbjct: 93  MVDMYVKCGKAEDAYNLFDKMPVRNIASWNAMIMGFSQIGSLDRVFNLFMGMRLVGTRPD 152

Query: 61  AATVIGLTRAVISAKSLRFLKAVHAIGIETGLDADTSVSNTWIAAYSKCGELQLAKMVFH 120
           AATVIGLTRAV+SAKSLRFLKAVHAIGIETGLDAD SVSNTWI+AYSKCGELQLAKMVFH
Sbjct: 153 AATVIGLTRAVLSAKSLRFLKAVHAIGIETGLDADNSVSNTWISAYSKCGELQLAKMVFH 212

Query: 121 GIQKTARSSVSWNSLIACYAHFGKYVDAVKSYKGLLCDGFKPDA---------------- 180
           GIQK ARSSVSWNSLIACYAHFGK VDAV SYKGLLCDGFKPDA                
Sbjct: 213 GIQKNARSSVSWNSLIACYAHFGKNVDAVNSYKGLLCDGFKPDACTIISLLCSCQQPEAL 272

Query: 181 -------------RCDSDISLINTLISMYSSGYSEVGRVDDALVLFNAM----------- 240
                         CDSDISLINTLISM    YS  G +  A +LF+ M           
Sbjct: 273 IYGSLIHGHGFQLGCDSDISLINTLISM----YSRCGDISSATILFDGMSIRTCVSWTAM 332

Query: 241 ----EETGEKPDIVTVLSLISGCGK----TGALGLGHWIDNYASL---HELKKD------ 300
                E G   D + + + +   G+       L L        +L   H +         
Sbjct: 333 ISGYSEVGRVDDALVLFNAMEETGEKPDIVTVLSLISGCGKTGALGLGHWIDNYASLHGL 392

Query: 301 ---VVVCNALIDMYAKCGSLNDAREIFYSLPNRTVVSWTAMIAACALNGEFREALDLFSL 322
              VVVCNALIDMYAKCGSLNDARE+FYSLPNRTVVSWTAMIAACALNGEFREALDLFSL
Sbjct: 393 KKDVVVCNALIDMYAKCGSLNDAREVFYSLPNRTVVSWTAMIAACALNGEFREALDLFSL 452

BLAST of Cucsa.104830 vs. NCBI nr
Match: gi|659080216|ref|XP_008440673.1| (PREDICTED: pentatricopeptide repeat-containing protein At4g19191, mitochondrial isoform X2 [Cucumis melo])

HSP 1 Score: 222.6 bits (566), Expect = 1.3e-54
Identity = 163/435 (37.47%), Postives = 211/435 (48.51%), Query Frame = 1

Query: 1   MVDMYVKCGKVDDAYNLFDKMPVRNIASWNAMIIGFSQIGSLDRVFNLFMGMRLVGTRPD 60
           ++ MY +CG +  A  LFD M +R   SW AMI                 G   VG   D
Sbjct: 297 LISMYSRCGDISSATILFDGMSIRTCVSWTAMI----------------SGYSEVGRVDD 356

Query: 61  AATVIGLTRAVISAKSLRFLKAVHAIGIETGLDADTSVSNTWIAAYSKCGELQLAKMV-- 120
           A  +                        ETG   D     + I+   K G L L   +  
Sbjct: 357 ALVLFNAME-------------------ETGEKPDIVTVLSLISGCGKTGALGLGHWIDN 416

Query: 121 ---FHGIQKTARSSVSWNSLIACYAHFGKYVDAVKSYKGLLCDGFKPDARCDSDISLINT 180
               HG++K     V  N+LI  YA  G   DA + +  L      P+    S  ++I  
Sbjct: 417 YASLHGLKKDV---VVCNALIDMYAKCGSLNDAREVFYSL------PNRTVVSWTAMI-- 476

Query: 181 LISMYSSGYSEVGRVDDALVLFNAMEETGEKPDIVTVLSLISGCGKTGALGLGH-WIDNY 240
                 +  +  G   +AL LF+ + E+G +P+ +T L+++  C   G L  G       
Sbjct: 477 ------AACALNGEFREALDLFSLLSESGIEPNNITFLAVLQACCHGGYLEKGRECFMMM 536

Query: 241 ASLHELKKDVVVCNALIDMYAKCGSLNDAREIFYSLPNRTVVS-WTAMIAACALNGEFRE 300
              + +   +   + +ID+  + G L +A E+   +P +     W A++ AC ++     
Sbjct: 537 TERYGINPGLDHYSCMIDLLGRKGKLIEALEVIQDMPMKPDEGIWGALLGACKIH----- 596

Query: 301 ALDLFSLLSESGIEPNNITLTRAYGVHCLVLVRFTTTWKSPRVAVSFVEMANIYASVGRW 360
                          NN+ +      H   L         PRVA SFVEMANIYA VGRW
Sbjct: 597 ---------------NNMEIGEYVSRHLFEL--------QPRVAASFVEMANIYALVGRW 651

Query: 361 DEVAAMRKTMRSNQMRKSPGKSVVQVNGMSHVFFVEDRSHHDSLLIYEALGNLAMQMKQK 420
           DEVAAMRK MRSNQMRKSPGKSVVQVNGMSHVFFVEDRSHHDSLLIYEALGNLAMQMKQK
Sbjct: 657 DEVAAMRKRMRSNQMRKSPGKSVVQVNGMSHVFFVEDRSHHDSLLIYEALGNLAMQMKQK 651

Query: 421 EFSSHAQRWVELDTI 429
           EFSSHAQR +ELDT+
Sbjct: 717 EFSSHAQRIIELDTM 651


HSP 2 Score: 452.6 bits (1163), Expect = 7.7e-124
Identity = 253/381 (66.40%), Postives = 268/381 (70.34%), Query Frame = 1

Query: 1   MVDMYVKCGKVDDAYNLFDKMPVRNIASWNAMIIGFSQIGSLDRVFNLFMGMRLVGTRPD 60
           MVDMYVKCGK +DAYNLFDKMPVRNIASWNAMI+GFSQIGSLDRVFNLFMGMRLVGTRPD
Sbjct: 93  MVDMYVKCGKAEDAYNLFDKMPVRNIASWNAMIMGFSQIGSLDRVFNLFMGMRLVGTRPD 152

Query: 61  AATVIGLTRAVISAKSLRFLKAVHAIGIETGLDADTSVSNTWIAAYSKCGELQLAKMVFH 120
           AATVIGLTRAV+SAKSLRFLKAVHAIGIETGLDAD SVSNTWI+AYSKCGELQLAKMVFH
Sbjct: 153 AATVIGLTRAVLSAKSLRFLKAVHAIGIETGLDADNSVSNTWISAYSKCGELQLAKMVFH 212

Query: 121 GIQKTARSSVSWNSLIACYAHFGKYVDAVKSYKGLLCDGFKPDA---------------- 180
           GIQK ARSSVSWNSLIACYAHFGK VDAV SYKGLLCDGFKPDA                
Sbjct: 213 GIQKNARSSVSWNSLIACYAHFGKNVDAVNSYKGLLCDGFKPDACTIISLLCSCQQPEAL 272

Query: 181 -------------RCDSDISLINTLISMYSSGYSEVGRVDDALVLFNAM----------- 240
                         CDSDISLINTLISM    YS  G +  A +LF+ M           
Sbjct: 273 IYGSLIHGHGFQLGCDSDISLINTLISM----YSRCGDISSATILFDGMSIRTCVSWTAM 332

Query: 241 ----EETGEKPDIVTVLSLISGCGK----TGALGLGHWIDNYASL---HELKKD------ 300
                E G   D + + + +   G+       L L        +L   H +         
Sbjct: 333 ISGYSEVGRVDDALVLFNAMEETGEKPDIVTVLSLISGCGKTGALGLGHWIDNYASLHGL 392

Query: 301 ---VVVCNALIDMYAKCGSLNDAREIFYSLPNRTVVSWTAMIAACALNGEFREALDLFSL 322
              VVVCNALIDMYAKCGSLNDARE+FYSLPNRTVVSWTAMIAACALNGEFREALDLFSL
Sbjct: 393 KKDVVVCNALIDMYAKCGSLNDAREVFYSLPNRTVVSWTAMIAACALNGEFREALDLFSL 452

BLAST of Cucsa.104830 vs. NCBI nr
Match: gi|659080214|ref|XP_008440672.1| (PREDICTED: pentatricopeptide repeat-containing protein At4g19191, mitochondrial isoform X1 [Cucumis melo])

HSP 1 Score: 222.2 bits (565), Expect = 1.7e-54
Identity = 163/434 (37.56%), Postives = 210/434 (48.39%), Query Frame = 1

Query: 1   MVDMYVKCGKVDDAYNLFDKMPVRNIASWNAMIIGFSQIGSLDRVFNLFMGMRLVGTRPD 60
           ++ MY +CG +  A  LFD M +R   SW AMI                 G   VG   D
Sbjct: 297 LISMYSRCGDISSATILFDGMSIRTCVSWTAMI----------------SGYSEVGRVDD 356

Query: 61  AATVIGLTRAVISAKSLRFLKAVHAIGIETGLDADTSVSNTWIAAYSKCGELQLAKMV-- 120
           A  +                        ETG   D     + I+   K G L L   +  
Sbjct: 357 ALVLFNAME-------------------ETGEKPDIVTVLSLISGCGKTGALGLGHWIDN 416

Query: 121 ---FHGIQKTARSSVSWNSLIACYAHFGKYVDAVKSYKGLLCDGFKPDARCDSDISLINT 180
               HG++K     V  N+LI  YA  G   DA + +  L      P+    S  ++I  
Sbjct: 417 YASLHGLKKDV---VVCNALIDMYAKCGSLNDAREVFYSL------PNRTVVSWTAMI-- 476

Query: 181 LISMYSSGYSEVGRVDDALVLFNAMEETGEKPDIVTVLSLISGCGKTGALGLGH-WIDNY 240
                 +  +  G   +AL LF+ + E+G +P+ +T L+++  C   G L  G       
Sbjct: 477 ------AACALNGEFREALDLFSLLSESGIEPNNITFLAVLQACCHGGYLEKGRECFMMM 536

Query: 241 ASLHELKKDVVVCNALIDMYAKCGSLNDAREIFYSLPNRTVVS-WTAMIAACALNGEFRE 300
              + +   +   + +ID+  + G L +A E+   +P +     W A++ AC ++     
Sbjct: 537 TERYGINPGLDHYSCMIDLLGRKGKLIEALEVIQDMPMKPDEGIWGALLGACKIH----- 596

Query: 301 ALDLFSLLSESGIEPNNITLTRAYGVHCLVLVRFTTTWKSPRVAVSFVEMANIYASVGRW 360
                          NN+ +      H   L         PRVA SFVEMANIYA VGRW
Sbjct: 597 ---------------NNMEIGEYVSRHLFEL--------QPRVAASFVEMANIYALVGRW 650

Query: 361 DEVAAMRKTMRSNQMRKSPGKSVVQVNGMSHVFFVEDRSHHDSLLIYEALGNLAMQMKQK 420
           DEVAAMRK MRSNQMRKSPGKSVVQVNGMSHVFFVEDRSHHDSLLIYEALGNLAMQMKQK
Sbjct: 657 DEVAAMRKRMRSNQMRKSPGKSVVQVNGMSHVFFVEDRSHHDSLLIYEALGNLAMQMKQK 650

Query: 421 EFSSHAQRWVELDT 428
           EFSSHAQR +ELDT
Sbjct: 717 EFSSHAQRIIELDT 650


HSP 2 Score: 339.0 bits (868), Expect = 1.2e-89
Identity = 175/369 (47.43%), Postives = 249/369 (67.48%), Query Frame = 1

Query: 1   MVDMYVKCGKVDDAYNLFDKMPVRNIASWNAMIIGFSQIGSLDRVFNLFMGMRLVGTRPD 60
           +VDMY+KC ++ DA+ +F+KMP R++A+WN+MI+G +Q+G  +RVF LF  MRL G + D
Sbjct: 51  VVDMYLKCDRLSDAHGVFEKMPARDVAAWNSMIVGLAQLGFTNRVFCLFHRMRLAGIQLD 110

Query: 61  AATVIGLTRAVIS-AKSLRFLKAVHAIGIETGLDADTSVSNTWIAAYSKCGELQLAKMVF 120
           + T++GLT+A+ S +K++  LKA+H++GI  GL+AD SV+NTWI+AY+KC +L  AK+VF
Sbjct: 111 SITIMGLTQAITSHSKNVELLKAIHSLGIRIGLEADISVANTWISAYAKCSDLDSAKVVF 170

Query: 121 HGIQKTARSSVSWNSLIACYAHFGK-----------YVDAVK----SYKGLLCDGFKPDA 180
            GI    R+ VSWNS+IA Y++F K            VD  +    +  GLL    +P A
Sbjct: 171 DGIDLDVRTVVSWNSMIAAYSNFEKSIDALNCYKRMLVDGYRPDSSTILGLLSSCAQPKA 230

Query: 181 R--------------CDSDISLINTLISMYS---------------------------SG 240
                          CDSDI++ NTLISMYS                           SG
Sbjct: 231 LLQGASVHCHGIQLGCDSDIAVANTLISMYSRCGDIFAARLLFDCVSCRTCVTWNVMISG 290

Query: 241 YSEVGRVDDALVLFNAMEETGEKPDIVTVLSLISGCGKTGALGLGHWIDNYASLHELKKD 300
           Y++ G +D+AL LF+AME  GEKPD+VT+LS+ISGC +TG+L +G WI+ YA  + L+ +
Sbjct: 291 YADKGDLDEALELFDAMETAGEKPDLVTMLSVISGCSQTGSLEVGKWINGYAFSNGLRDN 350

Query: 301 VVVCNALIDMYAKCGSLNDAREIFYSLPNRTVVSWTAMIAACALNGEFREALDLFSLLSE 313
            ++CNALIDMYAKCGS+NDAR++FY+LP +TVVSWT +I+ CALNGE +EAL+LF+ + E
Sbjct: 351 TIICNALIDMYAKCGSMNDARKLFYALPEKTVVSWTTIISGCALNGEVKEALNLFNRMLE 410

BLAST of Cucsa.104830 vs. NCBI nr
Match: gi|703111630|ref|XP_010099905.1| (hypothetical protein L484_020091 [Morus notabilis])

HSP 1 Score: 181.8 bits (460), Expect = 2.5e-42
Identity = 121/417 (29.02%), Postives = 198/417 (47.48%), Query Frame = 1

Query: 1   MVDMYVKCGKVDDAYNLFDKMPVRNIASWNAMIIGFSQIGSLDRVFNLFMGMRLVGTRPD 60
           ++ MY +CG +  A  LFD +  R   +WN MI G++  G LD    LF  M   G +PD
Sbjct: 256 LISMYSRCGDIFAARLLFDCVSCRTCVTWNVMISGYADKGDLDEALELFDAMETAGEKPD 315

Query: 61  AATVIGLTRAVISAKSLRFLKAVHAIGIETGLDADTSVSNTWIAAYSKCGELQLAKMVFH 120
             T++ +        SL   K ++      GL  +T + N  I  Y+KCG +  A+ +F+
Sbjct: 316 LVTMLSVISGCSQTGSLEVGKWINGYAFSNGLRDNTIICNALIDMYAKCGSMNDARKLFY 375

Query: 121 GIQKTARSSVSWNSLIACYAHFGKYVDAVKSYKGLLCDGFKPDARCDSDISLINTLISMY 180
            + +  ++ VSW ++I+  A  G+  +A+  +  +L  G KP     + ++ +  L +  
Sbjct: 376 ALPE--KTVVSWTTIISGCALNGEVKEALNLFNRMLESGLKP-----NHVTFLAVLQACA 435

Query: 181 SSGYSEVGRVDDALVLFNAMEETGEKPDIVTVLSLISGCGKTGALGLGHWIDNYASLHEL 240
            +G  E G                E  D++T +  I+ C           +D+Y      
Sbjct: 436 HAGLLEKGL---------------ECFDVMTKVFRINPC-----------LDHY------ 495

Query: 241 KKDVVVCNALIDMYAKCGSLNDAREIFYSLPNRTVVS-WTAMIAACALNGEFREALDLFS 300
                  + ++D+  + G + +A E+  ++P +     W+++++AC ++           
Sbjct: 496 -------SCVVDLLGRKGKIREAFELLENMPLKPDAGVWSSLLSACKIH----------- 555

Query: 301 LLSESGIEPNNITLTRAYGVHCLVLVRFTTTWKSPRVAVSFVEMANIYASVGRWDEVAAM 360
                     NI +      H   L         P  AV +VEMANIYAS G+WD VAA+
Sbjct: 556 ---------QNIEIAEYVSCHLFEL--------DPLAAVPYVEMANIYASDGKWDGVAAI 598

Query: 361 RKTMRSNQMRKSPGKSVVQVNGMSHVFFVEDRSHHDSLLIYEALGNLAMQMKQKEFS 417
           RK M+ N ++KS GKS+VQVNG  HVF VEDR H +SL IYE L +L + +K+ + S
Sbjct: 616 RKMMKCNSVKKSAGKSIVQVNGKRHVFAVEDRGHSESLCIYEMLDSLMLLLKEGDLS 598


HSP 2 Score: 312.8 bits (800), Expect = 9.5e-82
Identity = 178/373 (47.72%), Postives = 229/373 (61.39%), Query Frame = 1

Query: 1   MVDMYVKCGKVDDAYNLFDKMPVRNIASWNAMIIGFSQIGSLDRVFNLFMGMRLVGTRPD 60
           MVDMY KC ++D AY LFDKMP R++ASWNAMI+GF+Q+G L++V  LF  MRLVG + D
Sbjct: 95  MVDMYAKCDRLDCAYKLFDKMPDRDVASWNAMIVGFAQMGFLEKVLCLFYNMRLVGIQAD 154

Query: 61  AATVIGLTRAVISAKSLRFLKAVHAIGIETGLDADTSVSNTWIAAYSKCGELQLAKMVFH 120
             TV+GLT+A I AK L  LK+VH+ GI  G+DAD SV NTWI++Y+KC +L++A++VF 
Sbjct: 155 FVTVMGLTQAAIHAKHLSLLKSVHSFGIHIGVDADVSVCNTWISSYAKCDDLKMAELVFC 214

Query: 121 GIQKTARSSV-------------SWNSLIACYAHF---GKYVDAVKSYKGLLCDGFKPDA 180
           GI++  R+ V              ++  +  Y H    G  +D V +   LL     P+A
Sbjct: 215 GIEERLRTVVSWNSMVAGCTYGDKFDDSLNFYRHMMYNGFRLD-VTTVVSLLSSFVCPEA 274

Query: 181 RC--------------DSDISLINTLISMYSSGYSEVGRVDDALVLFNA----------- 240
                           D D+S+INTLISM    YS+ G +D A VLF+            
Sbjct: 275 LVQGRLVHSHGIHYGFDLDVSVINTLISM----YSKCGDIDSARVLFDGICDRTRVSWTA 334

Query: 241 --------------------MEETGEKPDIVTVLSLISGCGKTGALGLGHWIDNYASLHE 300
                               ME  GE PD+VTVLS+ISGCG++GAL LG W DNYA    
Sbjct: 335 MISGYAQKGDLDEALRLFFVMEAAGEIPDLVTVLSMISGCGQSGALELGKWFDNYACSGG 394

Query: 301 LKKDVVVCNALIDMYAKCGSLNDAREIFYSLPNRTVVSWTAMIAACALNGEFREALDLFS 313
           LK +V+VCNALIDMY+KCGS+ DARE+FY+LP +TVVSWT MIA CALNGEF EALDLF 
Sbjct: 395 LKDNVMVCNALIDMYSKCGSIGDARELFYALPEKTVVSWTTMIAGCALNGEFVEALDLFH 454

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
PP323_ARATH6.2e-8145.38Pentatricopeptide repeat-containing protein At4g19191, mitochondrial OS=Arabidop... [more]
PP169_ARATH2.1e-4430.94Pentatricopeptide repeat-containing protein At2g22410, mitochondrial OS=Arabidop... [more]
PP175_ARATH1.1e-4230.50Pentatricopeptide repeat-containing protein At2g29760, chloroplastic OS=Arabidop... [more]
PP311_ARATH2.8e-4130.77Pentatricopeptide repeat-containing protein At4g14820 OS=Arabidopsis thaliana GN... [more]
PP235_ARATH3.4e-3930.57Putative pentatricopeptide repeat-containing protein At3g15930 OS=Arabidopsis th... [more]
Match NameE-valueIdentityDescription
A0A0A0KK94_CUCSA7.2e-12968.50Uncharacterized protein OS=Cucumis sativus GN=Csa_6G501860 PE=4 SV=1[more]
A0A0A0KK94_CUCSA8.7e-5838.19Uncharacterized protein OS=Cucumis sativus GN=Csa_6G501860 PE=4 SV=1[more]
W9RJG3_9ROSA1.8e-4229.02Uncharacterized protein OS=Morus notabilis GN=L484_020091 PE=4 SV=1[more]
A0A068UX59_COFCA4.7e-2726.56Uncharacterized protein OS=Coffea canephora GN=GSCOC_T00037516001 PE=4 SV=1[more]
A0A0J8BD82_BETVU1.6e-2729.01Uncharacterized protein OS=Beta vulgaris subsp. vulgaris GN=BVRB_4g097410 PE=4 S... [more]
Match NameE-valueIdentityDescription
AT4G19191.13.5e-8245.38 Tetratricopeptide repeat (TPR)-like superfamily protein[more]
AT2G22410.11.2e-4530.94 SLOW GROWTH 1[more]
AT2G29760.16.4e-4430.50 Tetratricopeptide repeat (TPR)-like superfamily protein[more]
AT4G14820.11.6e-4230.77 Pentatricopeptide repeat (PPR) superfamily protein[more]
AT3G15930.11.9e-4030.57 Pentatricopeptide repeat (PPR) superfamily protein[more]
Match NameE-valueIdentityDescription
gi|449451649|ref|XP_004143574.1|1.0e-12868.50PREDICTED: pentatricopeptide repeat-containing protein At4g19191, mitochondrial ... [more]
gi|449451649|ref|XP_004143574.1|1.3e-5738.19PREDICTED: pentatricopeptide repeat-containing protein At4g19191, mitochondrial ... [more]
gi|659080216|ref|XP_008440673.1|1.3e-5437.47PREDICTED: pentatricopeptide repeat-containing protein At4g19191, mitochondrial ... [more]
gi|659080214|ref|XP_008440672.1|1.7e-5437.56PREDICTED: pentatricopeptide repeat-containing protein At4g19191, mitochondrial ... [more]
gi|703111630|ref|XP_010099905.1|2.5e-4229.02hypothetical protein L484_020091 [Morus notabilis][more]
The following terms have been associated with this gene:
Vocabulary: INTERPRO
TermDefinition
IPR002885Pentatricopeptide_repeat
IPR011990TPR-like_helical_dom_sf
Vocabulary: Molecular Function
TermDefinition
GO:0005515protein binding
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0008150 biological_process
cellular_component GO:0005575 cellular_component
molecular_function GO:0005515 protein binding

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Cucsa.104830.1Cucsa.104830.1mRNA


Analysis Name: InterPro Annotations of cucumber (Gy14)
Date Performed: 2017-01-17
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR002885Pentatricopeptide repeatPFAMPF01535PPRcoord: 28..53
score: 0.0027coord: 100..121
score: 0.13coord: 1..26
score: 4.8E-4coord: 245..269
score: 0.0019coord: 130..159
score: 0.
IPR002885Pentatricopeptide repeatPFAMPF13041PPR_2coord: 274..314
score: 8.7E-8coord: 171..220
score: 9.
IPR002885Pentatricopeptide repeatTIGRFAMsTIGR00756TIGR00756coord: 182..209
score: 2.2E-4coord: 1..26
score: 0.0027coord: 28..61
score: 0.0021coord: 245..269
score: 0.0011coord: 276..309
score: 2.8E-8coord: 130..164
score: 1.
IPR002885Pentatricopeptide repeatPROFILEPS51375PPRcoord: 95..125
score: 6.73coord: 336..370
score: 6.632coord: 169..207
score: 8.594coord: 128..162
score: 10.008coord: 1..24
score: 7.87coord: 243..273
score: 9.01coord: 274..308
score: 11.564coord: 25..59
score: 9
IPR011990Tetratricopeptide-like helical domainGENE3DG3DSA:1.25.40.10coord: 126..180
score: 1.8E-6coord: 246..412
score: 1.
NoneNo IPR availablePANTHERPTHR24015FAMILY NOT NAMEDcoord: 1..377
score: 1.7E
NoneNo IPR availablePANTHERPTHR24015:SF604SUBFAMILY NOT NAMEDcoord: 1..377
score: 1.7E