Cucsa.102780 (gene) Cucumber (Gy14) v1

Overview
NameCucsa.102780
Typegene
OrganismCucumis sativus (Cucumber (Gy14) v1)
DescriptionCytochrome P450
Locationscaffold00927 : 1736080 .. 1737698 (-)
Genome Viewer
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ACACTTACGTCCTCTATTCTTCTCTCTCACTTTTCTTTCTTCTTCTTGCTTTCAAATTCATCTCTCGATCTCTTCGACTTCGCAATAACTTACCGCCAAGTCCACCCTCTTTTCCGGTCATCGGACATCTCCATCTCTTGAAAAAGCCAATCCACCGAACTTTCAGCAACCTCTCTGCCAAATATGGCCCTGTTATGTCTCTCCGCCTTGGATCCAAGCTTGCAGTCGTTTTATCCTCCTCGGCGGCGGTGGAGGACTGCTTCACTAAAAACGACGTTGTTCTCGCCAACCGTCCACGTTTGCTCGTTGGAAAATACATCGGCTATAATTACACTACCATGATTGGGGCTCCATACGGCGACCATTGGCGGAACCTTCGTCGAATTGGCGCCATTGAGATCTTCTCTATATCTCGAATCAACAAATTCACGGACATCCGAAGGGATGAAGTGATGCGAATTGTTCGAAAACTGTCACATAATTCGATTCATCAATTCTCGAAAGTAGAAATTCAATCGGCGATGTCGGAGCTGACGTTCAACATCACGATGAGAATGGCGGCTGGTAAAAGGTATTATGGCGAGGACGTGACGAATGAGGAAGAGGCGAGGAGGTTTAGGGAGTTGATTAAAGAGATTGTGGCGGTAGGAGGAGTATCAAATCCAGGGGATTTCTTACCTGTATTGAATTGGATGTCGAAGGGTATTGAGAGGAAATTAATACAACTGGGGAAGAAAGTGGATGCGTTCTTGCAAGGGCTAATTGATGACCATCGTAAGACGAAGGAAGAAGGAAGGAACACGATGATCGATCACTTGCTTTCTCTACAACAATCGGAACCCGATCACTATAACGATCAGATAATCAAAGGATTTATATTGGTAATTAATAAATTCTCCCACTTCTCTATTTTATTTATCATTTTTTTTTGGAAGAAAGGTGGATTTCCACAAATTTTTAGTTAAAAAGTTGTAATAAACTTACCAGAAATATAATAAATTTTGTAGATATTACTAACGGCTGGATCCGACACATCCGCGGTGACAATGGAATGGGCCTTGAGTCATCTCCTAAACAATCCTCAAGTGTTAAAAAAGGCAAAAGAGGAGATAGACACTCAAATTGGACAAGAAAGACTCGTGGAGGAATCAGACATTTCTAAACTACCTTATCTTCAAAGAATCGTCTCTGAGACTCTTAGGTTGAATCCTGCGGCACCCATGTTGGTTCCCCATTATGCAACCAATGACTGCAAAATATGTGGGTATGATGTGCCACGAGACACAATGGTACTAGTCAATGCTTGGGCCATACATAGAGATTCAAACGAGTGGGAGGATTGCATGAGTTTTAAACCAGAGCGATACGAAAAATCAGAAGCTGTAGAGATACACAAGTTTTTGCCTTTTGGATTAGGAAGGCGTGCATGTCCTGGTTCTGCCATGGCACATAGAGTGATGGGTCTTACTTTAGCAACTCTGATTCAATGTTTTGAGTGGAAGAAAATTGGAGAAGAAGACATTGACATGAAGGAGTTGTCTGGTGCCATAATGACCAAGATGGTGCCATTGGAGGCCATGTGCAAAGCTCGACCCATCATCAACAACATTTTTTACTAA

mRNA sequence

ACACTTACGTCCTCTATTCTTCTCTCTCACTTTTCTTTCTTCTTCTTGCTTTCAAATTCATCTCTCGATCTCTTCGACTTCGCAATAACTTACCGCCAAGTCCACCCTCTTTTCCGGTCATCGGACATCTCCATCTCTTGAAAAAGCCAATCCACCGAACTTTCAGCAACCTCTCTGCCAAATATGGCCCTGTTATGTCTCTCCGCCTTGGATCCAAGCTTGCAGTCGTTTTATCCTCCTCGGCGGCGGTGGAGGACTGCTTCACTAAAAACGACGTTGTTCTCGCCAACCGTCCACGTTTGCTCGTTGGAAAATACATCGGCTATAATTACACTACCATGATTGGGGCTCCATACGGCGACCATTGGCGGAACCTTCGTCGAATTGGCGCCATTGAGATCTTCTCTATATCTCGAATCAACAAATTCACGGACATCCGAAGGGATGAAGTGATGCGAATTGTTCGAAAACTGTCACATAATTCGATTCATCAATTCTCGAAAGTAGAAATTCAATCGGCGATGTCGGAGCTGACGTTCAACATCACGATGAGAATGGCGGCTGGTAAAAGGTATTATGGCGAGGACGTGACGAATGAGGAAGAGGCGAGGAGGTTTAGGGAGTTGATTAAAGAGATTGTGGCGGTAGGAGGAGTATCAAATCCAGGGGATTTCTTACCTGTATTGAATTGGATGTCGAAGGGTATTGAGAGGAAATTAATACAACTGGGGAAGAAAGTGGATGCGTTCTTGCAAGGGCTAATTGATGACCATCGTAAGACGAAGGAAGAAGGAAGGAACACGATGATCGATCACTTGCTTTCTCTACAACAATCGGAACCCGATCACTATAACGATCAGATAATCAAAGGATTTATATTGATATTACTAACGGCTGGATCCGACACATCCGCGGTGACAATGGAATGGGCCTTGAGTCATCTCCTAAACAATCCTCAAGTGTTAAAAAAGGCAAAAGAGGAGATAGACACTCAAATTGGACAAGAAAGACTCGTGGAGGAATCAGACATTTCTAAACTACCTTATCTTCAAAGAATCGTCTCTGAGACTCTTAGGTTGAATCCTGCGGCACCCATGTTGGTTCCCCATTATGCAACCAATGACTGCAAAATATGTGGGTATGATGTGCCACGAGACACAATGGTACTAGTCAATGCTTGGGCCATACATAGAGATTCAAACGAGTGGGAGGATTGCATGAGTTTTAAACCAGAGCGATACGAAAAATCAGAAGCTGTAGAGATACACAAGTTTTTGCCTTTTGGATTAGGAAGGCGTGCATGTCCTGGTTCTGCCATGGCACATAGAGTGATGGGTCTTACTTTAGCAACTCTGATTCAATGTTTTGAGTGGAAGAAAATTGGAGAAGAAGACATTGACATGAAGGAGTTGTCTGGTGCCATAATGACCAAGATGGTGCCATTGGAGGCCATGTGCAAAGCTCGACCCATCATCAACAACATTTTTTACTAA

Coding sequence (CDS)

ATGTCTCTCCGCCTTGGATCCAAGCTTGCAGTCGTTTTATCCTCCTCGGCGGCGGTGGAGGACTGCTTCACTAAAAACGACGTTGTTCTCGCCAACCGTCCACGTTTGCTCGTTGGAAAATACATCGGCTATAATTACACTACCATGATTGGGGCTCCATACGGCGACCATTGGCGGAACCTTCGTCGAATTGGCGCCATTGAGATCTTCTCTATATCTCGAATCAACAAATTCACGGACATCCGAAGGGATGAAGTGATGCGAATTGTTCGAAAACTGTCACATAATTCGATTCATCAATTCTCGAAAGTAGAAATTCAATCGGCGATGTCGGAGCTGACGTTCAACATCACGATGAGAATGGCGGCTGGTAAAAGGTATTATGGCGAGGACGTGACGAATGAGGAAGAGGCGAGGAGGTTTAGGGAGTTGATTAAAGAGATTGTGGCGGTAGGAGGAGTATCAAATCCAGGGGATTTCTTACCTGTATTGAATTGGATGTCGAAGGGTATTGAGAGGAAATTAATACAACTGGGGAAGAAAGTGGATGCGTTCTTGCAAGGGCTAATTGATGACCATCGTAAGACGAAGGAAGAAGGAAGGAACACGATGATCGATCACTTGCTTTCTCTACAACAATCGGAACCCGATCACTATAACGATCAGATAATCAAAGGATTTATATTGATATTACTAACGGCTGGATCCGACACATCCGCGGTGACAATGGAATGGGCCTTGAGTCATCTCCTAAACAATCCTCAAGTGTTAAAAAAGGCAAAAGAGGAGATAGACACTCAAATTGGACAAGAAAGACTCGTGGAGGAATCAGACATTTCTAAACTACCTTATCTTCAAAGAATCGTCTCTGAGACTCTTAGGTTGAATCCTGCGGCACCCATGTTGGTTCCCCATTATGCAACCAATGACTGCAAAATATGTGGGTATGATGTGCCACGAGACACAATGGTACTAGTCAATGCTTGGGCCATACATAGAGATTCAAACGAGTGGGAGGATTGCATGAGTTTTAAACCAGAGCGATACGAAAAATCAGAAGCTGTAGAGATACACAAGTTTTTGCCTTTTGGATTAGGAAGGCGTGCATGTCCTGGTTCTGCCATGGCACATAGAGTGATGGGTCTTACTTTAGCAACTCTGATTCAATGTTTTGAGTGGAAGAAAATTGGAGAAGAAGACATTGACATGAAGGAGTTGTCTGGTGCCATAATGACCAAGATGGTGCCATTGGAGGCCATGTGCAAAGCTCGACCCATCATCAACAACATTTTTTACTAA

Protein sequence

MSLRLGSKLAVVLSSSAAVEDCFTKNDVVLANRPRLLVGKYIGYNYTTMIGAPYGDHWRNLRRIGAIEIFSISRINKFTDIRRDEVMRIVRKLSHNSIHQFSKVEIQSAMSELTFNITMRMAAGKRYYGEDVTNEEEARRFRELIKEIVAVGGVSNPGDFLPVLNWMSKGIERKLIQLGKKVDAFLQGLIDDHRKTKEEGRNTMIDHLLSLQQSEPDHYNDQIIKGFILILLTAGSDTSAVTMEWALSHLLNNPQVLKKAKEEIDTQIGQERLVEESDISKLPYLQRIVSETLRLNPAAPMLVPHYATNDCKICGYDVPRDTMVLVNAWAIHRDSNEWEDCMSFKPERYEKSEAVEIHKFLPFGLGRRACPGSAMAHRVMGLTLATLIQCFEWKKIGEEDIDMKELSGAIMTKMVPLEAMCKARPIINNIFY*
Homology
[Close]
BLAST of Cucsa.102780 vs. Swiss-Prot
Match: C81E8_MEDTR (Cytochrome P450 81E8 OS=Medicago truncatula GN=CYP81E8 PE=2 SV=1)

HSP 1 Score: 511.5 bits (1316), Expect = 8.8e-144
Identity = 253/435 (58.16%), Postives = 330/435 (75.86%), Query Frame = 1 

Query: 2   SLRLGSKLAVVLSSSAAVEDCFTKNDVVLANRPRLLVGKYIGYNYTTMIGAPYGDHWRNL 61
           SL  GS+L VV+SS    ++CFTKND+VLANRP  L GKYIGYN TT+  +PYGDHWRNL
Sbjct: 68  SLWFGSRLVVVVSSLTIAQECFTKNDIVLANRPHFLTGKYIGYNNTTVAQSPYGDHWRNL 127

Query: 62  RRIGAIEIFSISRINKFTDIRRDEVMRIVRKLSHNSIHQFSKVEIQSAMSELTFNITMRM 121
           RRI +IEI S  R+N F +IRRDE+MR+++KL+  S + F++VE++   SE+TFN  MRM
Sbjct: 128 RRILSIEILSSHRLNSFLEIRRDEIMRLIQKLAQKSYNGFTEVELRPMFSEMTFNTIMRM 187

Query: 122 AAGKRYYGED--VTNEEEARRFRELIKEIVAVGGVSNPGDFLPVLNWMS-KGIERKLIQL 181
            +GKRYYG D  V++ EEAR FR +IKE+V++GG +N GDFL  L W    G+E++L ++
Sbjct: 188 VSGKRYYGNDCDVSDVEEARLFRGIIKEVVSLGGANNVGDFLGFLRWFDFDGLEKRLKKI 247

Query: 182 GKKVDAFLQGLIDDHRKTKEEGRNTMIDHLLSLQQSEPDHYNDQIIKGFILILLTAGSDT 241
            K+ DAFLQGLID+HR  K    NTMIDHLL+ QQS+P++Y DQIIKG ++++L AG+DT
Sbjct: 248 SKRTDAFLQGLIDEHRFGKRNS-NTMIDHLLTQQQSQPEYYTDQIIKGLMVVMLLAGTDT 307

Query: 242 SAVTMEWALSHLLNNPQVLKKAKEEIDTQIGQERLVEESDISKLPYLQRIVSETLRLNPA 301
           S+VT+EWA+S+LLN+P+++KKAK E+DT IG +R V+E DISKLPYLQ IV ETLRL+ A
Sbjct: 308 SSVTIEWAMSNLLNHPEIMKKAKNELDTHIGHDRQVDEHDISKLPYLQSIVYETLRLHAA 367

Query: 302 APMLVPHYATNDCKICGYDVPRDTMVLVNAWAIHRDSNEWEDCMSFKPERYEKSEAVEIH 361
           AP+LVPH ++ D  + GY++P++T+++VNAW IHRD N W D   FKPER+EK    E++
Sbjct: 368 APLLVPHLSSEDFSLGGYNIPQNTILMVNAWVIHRDPNLWSDPTCFKPERFEKEG--EVN 427

Query: 362 KFLPFGLGRRACPGSAMAHRVMGLTLATLIQCFEWKKIGEEDIDMKELSGAIMTKMVPLE 421
           K L FGLGRRACPG  ++ R  GLTL  LIQCFEWK+IGEE IDM E  G    K   L 
Sbjct: 428 KLLSFGLGRRACPGENLSQRTEGLTLGLLIQCFEWKRIGEEKIDMVEAKGITAGKKTSLN 487

Query: 422 AMCKAR-PI-INNIF 432
           AMCK R P+ IN++F
Sbjct: 488 AMCKVRHPLKINDVF 499

[Close]
BLAST of Cucsa.102780 vs. Swiss-Prot
Match: C8D11_ARATH (Cytochrome P450 81D11 OS=Arabidopsis thaliana GN=CYP81D11 PE=2 SV=1)

HSP 1 Score: 498.4 bits (1282), Expect = 7.7e-140
Identity = 247/430 (57.44%), Postives = 320/430 (74.42%), Query Frame = 1 

Query: 2   SLRLGSKLAVVLSSSAAVEDCFTKNDVVLANRPRLLVGKYIGYNYTTMIGAPYGDHWRNL 61
           SL LGS+L  V+SS A  E+CFTKNDVVLANRP  LVGK+IGYN TTM+GA YGD WRNL
Sbjct: 78  SLSLGSRLVFVVSSHAVAEECFTKNDVVLANRPEFLVGKHIGYNSTTMVGAAYGDSWRNL 137

Query: 62  RRIGAIEIFSISRINKFTDIRRDEVMRIVRKLSHNSIHQFSKVEIQSAMSELTFNITMRM 121
           RRIG IEIFS  R+N F  IR+DE+ R++  L+ NS H F KVE++     LT N  +RM
Sbjct: 138 RRIGTIEIFSSLRLNSFVSIRQDEIRRLIICLAKNSQHGFVKVEMKPLFMCLTINNIIRM 197

Query: 122 AAGKRYYGEDVTNEEEARRFRELIKEIVAVGGVSNPGDFLPVLNWMSKGIERKLIQLGKK 181
            AGKR+YG+   N+ EA+  R+LI E+V  GG  N  D+ P+L +++   E+ + +L  +
Sbjct: 198 VAGKRFYGDGTENDNEAKHVRQLIAEVVVSGGAGNAADYFPILRYVTN-YEKHVKKLAGR 257

Query: 182 VDAFLQGLIDDHRKTKEEGRNTMIDHLLSLQQSEPDHYNDQIIKGFILILLTAGSDTSAV 241
           VD FLQ L+++ R  K +G NTMIDHLLSLQ+++PD+Y D IIKG IL+++ AG+DTSA 
Sbjct: 258 VDEFLQSLVNEKRVEKVKG-NTMIDHLLSLQETQPDYYTDVIIKGIILVMILAGTDTSAG 317

Query: 242 TMEWALSHLLNNPQVLKKAKEEIDTQIGQERLVEESDISKLPYLQRIVSETLRLNPAAPM 301
           T+EWA+S+LLN+P+VL+KAK EID QIG +RLVEE DI KLPYLQ IVSETLRL P APM
Sbjct: 318 TLEWAMSNLLNHPEVLRKAKTEIDDQIGVDRLVEEQDIVKLPYLQHIVSETLRLYPVAPM 377

Query: 302 LVPHYATNDCKICGYDVPRDTMVLVNAWAIHRDSNEWEDCMSFKPERYEKSEAVEIHKFL 361
           L+PH A+ DC + GYDVPR T++LVNAWAIHRD   WE+   FKPER+EK    E  K +
Sbjct: 378 LLPHLASEDCIVDGYDVPRGTIILVNAWAIHRDPKLWEEPEKFKPERFEKKG--EDKKLM 437

Query: 362 PFGLGRRACPGSAMAHRVMGLTLATLIQCFEWKKIGEEDIDMKEL-SGAIMTKMVPLEAM 421
           PFG+GRR+CPGS +A R++ L L +L+QCFEW+++ E+ +DM+E   G  M K   L+AM
Sbjct: 438 PFGIGRRSCPGSGLAQRLVTLALGSLVQCFEWERVEEKYLDMRESEKGTTMRKATSLQAM 497

Query: 422 CKARPIINNI 431
           CKARPI++ +
Sbjct: 498 CKARPIVHKV 503

[Close]
BLAST of Cucsa.102780 vs. Swiss-Prot
Match: C81D1_ARATH (Cytochrome P450 81D1 OS=Arabidopsis thaliana GN=CYP81D1 PE=2 SV=1)

HSP 1 Score: 498.4 bits (1282), Expect = 7.7e-140
Identity = 246/431 (57.08%), Postives = 328/431 (76.10%), Query Frame = 1 

Query: 1   MSLRLGSKLAVVLSS-SAAVEDCFTKNDVVLANRPRLLVGKYIGYNYTTMIGAPYGDHWR 60
           MSLRLGS+L  V+SS   A E+CF KNDVVLANRP++++GK++GYN T MI APYGDHWR
Sbjct: 74  MSLRLGSRLVYVVSSHKVAAEECFGKNDVVLANRPQVIIGKHVGYNNTNMIAAPYGDHWR 133

Query: 61  NLRRIGAIEIFSISRINKFTDIRRDEVMRIVRKLSHNSIHQFSKVEIQSAMSELTFNITM 120
           NLRR+  IEIFS  R+N F  +R DEV R++ +LS  +  + + VE++  + +LTFN  M
Sbjct: 134 NLRRLCTIEIFSTHRLNCFLYVRTDEVRRLISRLSRLAGTKKTVVELKPMLMDLTFNNIM 193

Query: 121 RMAAGKRYYGEDVTNEEEARRFRELIKEIVAVGGVSNPGDFLPVLNWMSKGIERKLIQLG 180
           RM  GKRYYGE+ T+EEEA+R R+L+ ++ A     N  D++P+L   S   E ++ +LG
Sbjct: 194 RMMTGKRYYGEETTDEEEAKRVRKLVADVGANTSSGNAVDYVPILRLFSS-YENRVKKLG 253

Query: 181 KKVDAFLQGLIDDHRKTKEEGRNTMIDHLLSLQQSEPDHYNDQIIKGFILILLTAGSDTS 240
           ++ D FLQGLIDD R  +E G  TMIDHLL LQ+S+ ++Y DQIIKG ILI++ AG++TS
Sbjct: 254 EETDKFLQGLIDDKRGQQETG-TTMIDHLLVLQKSDIEYYTDQIIKGIILIMVIAGTNTS 313

Query: 241 AVTMEWALSHLLNNPQVLKKAKEEIDTQIGQERLVEESDISKLPYLQRIVSETLRLNPAA 300
           AVT+EWALS+LLN+P V+ KA++EID ++G +RL+EE+D+S+LPYL+ IV ETLRL+PA 
Sbjct: 314 AVTLEWALSNLLNHPDVISKARDEIDNRVGLDRLIEEADLSELPYLKNIVLETLRLHPAT 373

Query: 301 PMLVPHYATNDCKICGYDVPRDTMVLVNAWAIHRDSNEWEDCMSFKPERYEKSEAVEIHK 360
           P+LVPH A+ DCKI  YD+PR T +LVNAWAIHRD N W+D  SFKPER+EK E  E  K
Sbjct: 374 PLLVPHMASEDCKIGSYDMPRGTTLLVNAWAIHRDPNTWDDPDSFKPERFEKEE--EAQK 433

Query: 361 FLPFGLGRRACPGSAMAHRVMGLTLATLIQCFEWKKIGEEDIDMKELSGAIMTKMVPLEA 420
            L FGLGRRACPGS +A R++GL L +LIQCFEW+++G  ++DMKE  G  + K +PL+A
Sbjct: 434 LLAFGLGRRACPGSGLAQRIVGLALGSLIQCFEWERVGNVEVDMKEGVGNTVPKAIPLKA 493

Query: 421 MCKARPIINNI 431
           +CKARP ++ I
Sbjct: 494 ICKARPFLHKI 500

[Close]
BLAST of Cucsa.102780 vs. Swiss-Prot
Match: C81E1_GLYEC (Isoflavone 2'-hydroxylase OS=Glycyrrhiza echinata GN=CYP81E1 PE=1 SV=2)

HSP 1 Score: 493.4 bits (1269), Expect = 2.5e-138
Identity = 233/433 (53.81%), Postives = 330/433 (76.21%), Query Frame = 1 

Query: 2   SLRLGSKLAVVLSSSAAVEDCFTKNDVVLANRPRLLVGKYIGYNYTTMIGAPYGDHWRNL 61
           SL  GS+L VV+SS++  + CFTKNDVVLANRPR L GKYI YNYTT+    YG+HWRNL
Sbjct: 68  SLWFGSRLVVVVSSASEFQQCFTKNDVVLANRPRFLSGKYIFYNYTTLGSTSYGEHWRNL 127

Query: 62  RRIGAIEIFSISRINKFTDIRRDEVMRIVRKLSHNSIHQFSKVEIQSAMSELTFNITMRM 121
           RRI A+++ S  RIN F+ IRRDE  R++ +L+ +S   F+++E+ S + ++TFN  MRM
Sbjct: 128 RRITALDVLSNHRINSFSGIRRDETQRLITRLADDSSTNFAEMELSSRLYDMTFNNIMRM 187

Query: 122 AAGKRYYGED--VTNEEEARRFRELIKEIVAVGGVSNPGDFLPVLNWMS-KGIERKLIQL 181
            +GKRYYGED   ++ +EA +FR+++ E++ + G +N  DF+P+L ++  + +E++L  +
Sbjct: 188 ISGKRYYGEDCDTSDLQEASQFRDMVSELLQLSGANNKTDFMPLLRFLDFENLEKRLKDI 247

Query: 182 GKKVDAFLQGLIDDHRKTKEEGRNTMIDHLLSLQQSEPDHYNDQIIKGFILILLTAGSDT 241
             K DAFL+GLI++HR TK+E  NTMIDHLL+LQ S+P++Y DQIIKG  L +L AG+D+
Sbjct: 248 SGKTDAFLRGLIEEHR-TKKERANTMIDHLLNLQDSQPEYYTDQIIKGLALAMLLAGTDS 307

Query: 242 SAVTMEWALSHLLNNPQVLKKAKEEIDTQIGQERLVEESDISKLPYLQRIVSETLRLNPA 301
           SAVT+EW++S+LLN+P+VLKK K+E+DT +GQ+RLV+ESD+ KL YL+ +++ETLRL   
Sbjct: 308 SAVTLEWSMSNLLNHPEVLKKVKDELDTHVGQDRLVDESDLPKLTYLKNVINETLRLYTP 367

Query: 302 APMLVPHYATNDCKICGYDVPRDTMVLVNAWAIHRDSNEWEDCMSFKPERYEKSEAVEIH 361
           AP+L+PH  +++C I GY VP+DT+VL+NAWAIHRD   W +  +FKPER+EK    E+ 
Sbjct: 368 APLLLPHSTSDECNIGGYKVPQDTIVLINAWAIHRDPELWTEATTFKPERFEKKG--ELE 427

Query: 362 KFLPFGLGRRACPGSAMAHRVMGLTLATLIQCFEWKKIGEEDIDMKELSGAIMTKMVPLE 421
           K + FG+GRRACPG  +A R + +TLA LIQCF+WK I  + ID+ E  G  +TK+VPL+
Sbjct: 428 KLIAFGMGRRACPGEGLAIRAISMTLALLIQCFDWKLINGDKIDLAERDGFTLTKLVPLK 487

Query: 422 AMCKARPIINNIF 432
           AMCK+RP+IN +F
Sbjct: 488 AMCKSRPVINKVF 497

[Close]
BLAST of Cucsa.102780 vs. Swiss-Prot
Match: C81E9_MEDTR (Isoflavone 3'-hydroxylase (Fragment) OS=Medicago truncatula GN=CYP81E9 PE=1 SV=1)

HSP 1 Score: 492.3 bits (1266), Expect = 5.5e-138
Identity = 235/436 (53.90%), Postives = 327/436 (75.00%), Query Frame = 1 

Query: 2   SLRLGSKLAVVLSSSAAVEDCFTKNDVVLANRPRLLVGKYIGYNYTTMIGAPYGDHWRNL 61
           SL  GS+L VV+SS +   +CFTKND++LANRPR L GKYI YNYTT+  A YGDHWRNL
Sbjct: 66  SLWFGSRLVVVVSSPSLAHECFTKNDIILANRPRFLTGKYIFYNYTTLGSASYGDHWRNL 125

Query: 62  RRIGAIEIFSISRINKFTDIRRDEVMRIVRKLSHNSIHQ---FSKVEIQSAMSELTFNIT 121
           RRI  I++ S +R+N F  +RRDE  R+++KL  + + +   F+KVE++  ++E+TFN  
Sbjct: 126 RRITTIDVLSNNRLNSFLGVRRDETNRLIQKLLKDVVSEGFGFTKVELRPRLTEMTFNAM 185

Query: 122 MRMAAGKRYYGED--VTNEEEARRFRELIKEIVAVGGVSNPGDFLPVLNWMS-KGIERKL 181
           MRM +GKRYYG+D  V++ EEA++FRE+I E++++ G +N GDFLP+L  +    +E++ 
Sbjct: 186 MRMISGKRYYGDDGDVSDVEEAKQFREIISEMMSLLGANNKGDFLPLLRVVDLDNLEKRC 245

Query: 182 IQLGKKVDAFLQGLIDDHRKTK-EEGRNTMIDHLLSLQQSEPDHYNDQIIKGFILILLTA 241
            ++ K+ +AFL+GLI++HR+        TMIDHLL L +S+P++Y+D +IKG I  +L A
Sbjct: 246 KRIAKRSNAFLEGLIEEHRRGNIHSDGGTMIDHLLKLSESQPEYYSDHLIKGLIQGMLLA 305

Query: 242 GSDTSAVTMEWALSHLLNNPQVLKKAKEEIDTQIGQERLVEESDISKLPYLQRIVSETLR 301
           G+DTSAVT+EW +S LLN+P+VLKKAKEE+DTQIG+ +LV+E D+SKLPYLQ I+SETLR
Sbjct: 306 GTDTSAVTIEWVMSELLNHPEVLKKAKEELDTQIGKNKLVDEQDLSKLPYLQNIISETLR 365

Query: 302 LNPAAPMLVPHYATNDCKICGYDVPRDTMVLVNAWAIHRDSNEWEDCMSFKPERYEKSEA 361
           L+P AP+L+PHY++ DC I  ++VP+DT++L N W IHRD   W D +SFKPER+EK E 
Sbjct: 366 LHPPAPLLLPHYSSEDCTIGEFNVPKDTIILTNVWGIHRDPKHWNDALSFKPERFEKEE- 425

Query: 362 VEIHKFLPFGLGRRACPGSAMAHRVMGLTLATLIQCFEWKKIGEEDIDMKELSGAIMTKM 421
            E++K + FGLGRRACPG ++A R +G T+  LIQCFEW++  EE +DM E  G  M   
Sbjct: 426 -EVNKVMAFGLGRRACPGLSLAQRTVGFTVGLLIQCFEWERESEEKLDMMEGKGITMPMK 485

Query: 422 VPLEAMCKARPIINNI 431
           +PL AMCKA PI N++
Sbjct: 486 IPLRAMCKALPIANDV 499

[Close]
BLAST of Cucsa.102780 vs. TrEMBL
Match: A0A0A0KFV2_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_6G492250 PE=3 SV=1)

HSP 1 Score: 865.9 bits (2236), Expect = 2.0e-248
Identity = 432/432 (100.00%), Postives = 432/432 (100.00%), Query Frame = 1 

Query: 1   MSLRLGSKLAVVLSSSAAVEDCFTKNDVVLANRPRLLVGKYIGYNYTTMIGAPYGDHWRN 60
           MSLRLGSKLAVVLSSSAAVEDCFTKNDVVLANRPRLLVGKYIGYNYTTMIGAPYGDHWRN
Sbjct: 69  MSLRLGSKLAVVLSSSAAVEDCFTKNDVVLANRPRLLVGKYIGYNYTTMIGAPYGDHWRN 128

Query: 61  LRRIGAIEIFSISRINKFTDIRRDEVMRIVRKLSHNSIHQFSKVEIQSAMSELTFNITMR 120
           LRRIGAIEIFSISRINKFTDIRRDEVMRIVRKLSHNSIHQFSKVEIQSAMSELTFNITMR
Sbjct: 129 LRRIGAIEIFSISRINKFTDIRRDEVMRIVRKLSHNSIHQFSKVEIQSAMSELTFNITMR 188

Query: 121 MAAGKRYYGEDVTNEEEARRFRELIKEIVAVGGVSNPGDFLPVLNWMSKGIERKLIQLGK 180
           MAAGKRYYGEDVTNEEEARRFRELIKEIVAVGGVSNPGDFLPVLNWMSKGIERKLIQLGK
Sbjct: 189 MAAGKRYYGEDVTNEEEARRFRELIKEIVAVGGVSNPGDFLPVLNWMSKGIERKLIQLGK 248

Query: 181 KVDAFLQGLIDDHRKTKEEGRNTMIDHLLSLQQSEPDHYNDQIIKGFILILLTAGSDTSA 240
           KVDAFLQGLIDDHRKTKEEGRNTMIDHLLSLQQSEPDHYNDQIIKGFILILLTAGSDTSA
Sbjct: 249 KVDAFLQGLIDDHRKTKEEGRNTMIDHLLSLQQSEPDHYNDQIIKGFILILLTAGSDTSA 308

Query: 241 VTMEWALSHLLNNPQVLKKAKEEIDTQIGQERLVEESDISKLPYLQRIVSETLRLNPAAP 300
           VTMEWALSHLLNNPQVLKKAKEEIDTQIGQERLVEESDISKLPYLQRIVSETLRLNPAAP
Sbjct: 309 VTMEWALSHLLNNPQVLKKAKEEIDTQIGQERLVEESDISKLPYLQRIVSETLRLNPAAP 368

Query: 301 MLVPHYATNDCKICGYDVPRDTMVLVNAWAIHRDSNEWEDCMSFKPERYEKSEAVEIHKF 360
           MLVPHYATNDCKICGYDVPRDTMVLVNAWAIHRDSNEWEDCMSFKPERYEKSEAVEIHKF
Sbjct: 369 MLVPHYATNDCKICGYDVPRDTMVLVNAWAIHRDSNEWEDCMSFKPERYEKSEAVEIHKF 428

Query: 361 LPFGLGRRACPGSAMAHRVMGLTLATLIQCFEWKKIGEEDIDMKELSGAIMTKMVPLEAM 420
           LPFGLGRRACPGSAMAHRVMGLTLATLIQCFEWKKIGEEDIDMKELSGAIMTKMVPLEAM
Sbjct: 429 LPFGLGRRACPGSAMAHRVMGLTLATLIQCFEWKKIGEEDIDMKELSGAIMTKMVPLEAM 488

Query: 421 CKARPIINNIFY 433
           CKARPIINNIFY
Sbjct: 489 CKARPIINNIFY 500

[Close]
BLAST of Cucsa.102780 vs. TrEMBL
Match: A0A0A0KJM0_CUCSA (Cytochrome P450 OS=Cucumis sativus GN=Csa_6G492230 PE=3 SV=1)

HSP 1 Score: 647.9 bits (1670), Expect = 8.8e-183
Identity = 306/433 (70.67%), Postives = 380/433 (87.76%), Query Frame = 1 

Query: 1   MSLRLGSKLAVVLSSSAAVEDCFTKNDVVLANRPRLLVGKYIGYNYTTMIGAPYGDHWRN 60
           +SL  GS+L VV+SS  AVE+CFTKND+VLANRPRLLVGK++GYN+TTM+ +PYGDHWRN
Sbjct: 64  ISLWFGSRLVVVVSSLPAVEECFTKNDIVLANRPRLLVGKHVGYNHTTMVASPYGDHWRN 123

Query: 61  LRRIGAIEIFSISRINKFTDIRRDEVMRIVRKLSHNSIHQFSKVEIQSAMSELTFNITMR 120
           LRRIGAIEIFS SR+NKF   R+DEV R+++KLS NS++ FSKVE+QS++SELTFNI+MR
Sbjct: 124 LRRIGAIEIFSASRLNKFAGTRKDEVERLLKKLSRNSMYGFSKVEMQSSISELTFNISMR 183

Query: 121 MAAGKRYYGEDVTNEEEARRFRELIKEIVAVGGVSNPGDFLPVLNWMSKGIERKLIQLGK 180
           MAAGKRY+GE+V + EEAR+ RELIK+IV++GGVSNPGDF+P++NW+  G +RK+ ++ K
Sbjct: 184 MAAGKRYFGEEVRDVEEARQSRELIKQIVSMGGVSNPGDFIPMMNWIPNGFKRKVWRVAK 243

Query: 181 KVDAFLQGLIDDHRKTKEEGRNTMIDHLLSLQQSEPDHYNDQIIKGFILILLTAGSDTSA 240
           ++D FLQGLID+HR  KEE RNTMIDHLLSLQ++EP++Y DQIIKG IL+LL AG+DTSA
Sbjct: 244 RMDGFLQGLIDEHRSNKEEERNTMIDHLLSLQETEPEYYGDQIIKGIILVLLLAGTDTSA 303

Query: 241 VTMEWALSHLLNNPQVLKKAKEEIDTQIGQERLVEESDISKLPYLQRIVSETLRLNPAAP 300
           VT+EWAL+HLLNNP+VLKKA+EE+DTQIG++RLVEESD+SKLPYLQ I+SETLRLNPAAP
Sbjct: 304 VTIEWALAHLLNNPEVLKKAREELDTQIGEKRLVEESDVSKLPYLQGIISETLRLNPAAP 363

Query: 301 MLVPHYATNDCKICGYDVPRDTMVLVNAWAIHRDSNEWEDCMSFKPERYEKSEAVEIH-- 360
           MLVPH  +NDC I GY +PRDT+VLVNAWAIHRD ++WE+   FKPER++KSE+++ H  
Sbjct: 364 MLVPHLTSNDCTISGYKIPRDTIVLVNAWAIHRDPHQWEEPTLFKPERHQKSESIDHHIS 423

Query: 361 KFLPFGLGRRACPGSAMAHRVMGLTLATLIQCFEWKKIGEEDIDMKELSGAIMTKMVPLE 420
           K +PFG+GRRACPGS MA RV+GLTLA LIQC+EW++IG+E +DM E  G  M KMVPLE
Sbjct: 424 KLIPFGVGRRACPGSGMAQRVVGLTLAALIQCYEWERIGDEKVDMSEGRGVTMPKMVPLE 483

Query: 421 AMCKARPIINNIF 432
           AMCK RPII+NIF
Sbjct: 484 AMCKPRPIIHNIF 496

[Close]
BLAST of Cucsa.102780 vs. TrEMBL
Match: A0A0A0KLF2_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_6G492220 PE=3 SV=1)

HSP 1 Score: 636.3 bits (1640), Expect = 2.6e-179
Identity = 299/434 (68.89%), Postives = 378/434 (87.10%), Query Frame = 1 

Query: 1   MSLRLGSKLAVVLSSSAAVEDCFTKNDVVLANRPRLLVGKYIGYNYTTMIGAPYGDHWRN 60
           +SLR GS+L VV+SS  AVE+CFTKND+VLANRPRLL GK+IGYN+TTM+G+PYGDHWRN
Sbjct: 64  ISLRFGSRLVVVVSSLPAVEECFTKNDIVLANRPRLLFGKHIGYNHTTMVGSPYGDHWRN 123

Query: 61  LRRIGAIEIFSISRINKFTDIRRDEVMRIVRKLSHNSIHQFSKVEIQSAMSELTFNITMR 120
           LRRIGAIEIFS SR+NKF   R+DEV R+++KLS NS+H +SKVE+QS++SELTFNI+MR
Sbjct: 124 LRRIGAIEIFSASRLNKFAGTRKDEVERLLKKLSRNSMHGYSKVEMQSSISELTFNISMR 183

Query: 121 MAAGKRYYGEDVTNEEEARRFRELIKEIVAVGGVSNPGDFLPVLNWMSKGIERKLIQLGK 180
           MAAGKRY+G++V + EEAR+ RELIK+IV++GGVSNPGDF+P++NW+  G +RK+ +LGK
Sbjct: 184 MAAGKRYFGDEVRDVEEARQSRELIKQIVSMGGVSNPGDFIPMMNWVPNGYKRKVSRLGK 243

Query: 181 KVDAFLQGLIDDHRKTKEEGRNTMIDHLLSLQQSEPDHYNDQIIKGFILILLTAGSDTSA 240
           ++D FLQGLID+HR  KEE RNTMIDHLLSLQ++EP++Y D+IIKG +L+LL AG+DTSA
Sbjct: 244 RMDRFLQGLIDEHRSNKEEERNTMIDHLLSLQETEPEYYGDEIIKGIVLVLLLAGTDTSA 303

Query: 241 VTMEWALSHLLNNPQVLKKAKEEIDTQIGQERLVEESDISKLPYLQRIVSETLRLNPAAP 300
           VT+EWAL+HLLNNP+VLKKA+EE+D QIG+++L EE D+SKLPYLQ I+ ETLRLNPAAP
Sbjct: 304 VTIEWALAHLLNNPEVLKKAREELDNQIGEKQLAEELDVSKLPYLQGIIYETLRLNPAAP 363

Query: 301 MLVPHYATNDCKICGYDVPRDTMVLVNAWAIHRDSNEWEDCMSFKPERYEKSEAVEIH-- 360
           MLVPH  +N+C I  Y++PRDT+VLVNAWAIHRD ++WE+   FKPER++  E+ + H  
Sbjct: 364 MLVPHLTSNNCTISEYEIPRDTIVLVNAWAIHRDPSQWEEPTLFKPERHQNLESSDHHQI 423

Query: 361 -KFLPFGLGRRACPGSAMAHRVMGLTLATLIQCFEWKKIGEEDIDMKELSGAIMTKMVPL 420
            K +PFG+GRRACPGS+MA RV+GLTLATLIQC+EW++IGEE +DM E  G  M K+VPL
Sbjct: 424 PKMIPFGVGRRACPGSSMAQRVVGLTLATLIQCYEWERIGEEKVDMTEGRGVTMPKVVPL 483

Query: 421 EAMCKARPIINNIF 432
           EAMCKARPI++NIF
Sbjct: 484 EAMCKARPIVHNIF 497

[Close]
BLAST of Cucsa.102780 vs. TrEMBL
Match: A0A061EFX4_THECC (Cytochrome P450, family 81, subfamily D, polypeptide 8, putative OS=Theobroma cacao GN=TCM_019137 PE=3 SV=1)

HSP 1 Score: 573.5 bits (1477), Expect = 2.1e-160
Identity = 281/433 (64.90%), Postives = 354/433 (81.76%), Query Frame = 1 

Query: 2   SLRLGSKLAVVLSSSAAVEDCFTKNDVVLANRPRLLVGKYIGYNYTTMIGAPYGDHWRNL 61
           SLR GS+L VV+SS  A  +CFTKND+VLANRP+LL+GKY+GYN++T++G+PYGDHWRNL
Sbjct: 70  SLRFGSRLVVVVSSPTAAGECFTKNDIVLANRPKLLIGKYLGYNWSTVVGSPYGDHWRNL 129

Query: 62  RRIGAIEIFSISRINKFTDIRRDEVMRIVRKLSH--NSIHQFSKVEIQSAMSELTFNITM 121
           RRI AIEIFS SR+N F  IR+DEV R++ KLS   NS  +F+KVE++S   +LTFN  M
Sbjct: 130 RRISAIEIFSSSRLNAFLSIRKDEVKRLLLKLSSDDNSGQEFTKVELRSLFVDLTFNNIM 189

Query: 122 RMAAGKRYYGED-VTNEEEARRFRELIKEIVAVGGVSNPGDFLPVLNWMS-KGIERKLIQ 181
           RM AGKRYYG D VT+E EA+ FREL+KE +A GGV++PGDFLP+LNW+  +   +KL+ 
Sbjct: 190 RMVAGKRYYGGDSVTDEGEAKEFRELMKESLASGGVAHPGDFLPILNWIGGRSYLKKLMN 249

Query: 182 LGKKVDAFLQGLIDDHRKTKEEGRNTMIDHLLSLQQSEPDHYNDQIIKGFILILLTAGSD 241
           LG+++D FLQ LID+ R  K +G NTMIDHLLSLQQ+EPD+Y DQ+IKG IL++L AG+D
Sbjct: 250 LGERMDRFLQKLIDEVR-AKRQG-NTMIDHLLSLQQTEPDYYTDQLIKGLILVMLLAGTD 309

Query: 242 TSAVTMEWALSHLLNNPQVLKKAKEEIDTQIGQERLVEESDISKLPYLQRIVSETLRLNP 301
           TSAVT+EWA+S+LLNNP VLKKA+ E+D+QIGQE L++E D+SKL YLQ I+ ETLRLNP
Sbjct: 310 TSAVTLEWAMSNLLNNPDVLKKARTELDSQIGQENLIDEPDVSKLQYLQSIIFETLRLNP 369

Query: 302 AAPMLVPHYATNDCKICGYDVPRDTMVLVNAWAIHRDSNEWEDCMSFKPERYEKSEAVEI 361
           AAP+L+PH A+ DCKIC YDVPRDT+VL+NAWAIHRDS  WED  SFKPERY   E  E 
Sbjct: 370 AAPLLLPHMASTDCKICEYDVPRDTIVLINAWAIHRDSTLWEDPTSFKPERYGNGER-ES 429

Query: 362 HKFLPFGLGRRACPGSAMAHRVMGLTLATLIQCFEWKKIGEEDIDMKELSGAIMTKMVPL 421
           +K +PFGLGRRACPG+ +A RV+GLTL +LIQCFEW+++ E+++DM E  G  M K+V L
Sbjct: 430 NKLMPFGLGRRACPGAGLAQRVVGLTLGSLIQCFEWERVSEKEVDMAEGDGTTMPKVVAL 489

Query: 422 EAMCKARPIINNI 431
           EAMCKARPI+N +
Sbjct: 490 EAMCKARPIVNKV 499

[Close]
BLAST of Cucsa.102780 vs. TrEMBL
Match: W9R984_9ROSA (Putative receptor-like protein kinase OS=Morus notabilis GN=L484_009298 PE=4 SV=1)

HSP 1 Score: 568.9 bits (1465), Expect = 5.2e-159
Identity = 265/427 (62.06%), Postives = 347/427 (81.26%), Query Frame = 1 

Query: 2   SLRLGSKLAVVLSSSAAVEDCFTKNDVVLANRPRLLVGKYIGYNYTTMIGAPYGDHWRNL 61
           SLR GS+  VV+SS +AVE+C TKNDVVLANRP L+  K+I YNYTT++ +PYGDHWRNL
Sbjct: 70  SLRFGSRRVVVVSSPSAVEECCTKNDVVLANRPPLITAKHISYNYTTIVSSPYGDHWRNL 129

Query: 62  RRIGAIEIFSISRINKFTDIRRDEVMRIVRKLSHNSIHQFSKVEIQSAMSELTFNITMRM 121
           RRIG ++IFS SR+N F  +RRDEV R++R++S NS+    KVE++S +SELT N+ MRM
Sbjct: 130 RRIGTLQIFSSSRLNVFRGLRRDEVKRLLRRISQNSLRGSGKVELKSMLSELTLNVIMRM 189

Query: 122 AAGKRYYGEDVTNEEEARRFRELIKEIVAVGGVSNPGDFLPVLNWMSKGIERKLIQLGKK 181
            AGKRYYG+DV++++EA++F+E+IKE+   GG +NP DF+P+LNW+  G E+K+ +L  +
Sbjct: 190 VAGKRYYGDDVSDQDEAKKFKEVIKEVSENGGGANPADFVPILNWIWGGFEKKMKRLAMR 249

Query: 182 VDAFLQGLIDDHRKTKEEGRNTMIDHLLSLQQSEPDHYNDQIIKGFILILLTAGSDTSAV 241
            D  LQ LID+HR +++E +NTMIDHLLSLQ+S+P++Y DQIIKGF LI+L AG+DTS+V
Sbjct: 250 TDTILQSLIDEHR-SRKESKNTMIDHLLSLQKSQPEYYTDQIIKGFALIMLLAGTDTSSV 309

Query: 242 TMEWALSHLLNNPQVLKKAKEEIDTQIGQERLVEESDISKLPYLQRIVSETLRLNPAAPM 301
           T+EWA+S+LLN+P +LKKAK E+D Q+GQ+ LVEESD+SKL YLQ I+SETLRL PA P+
Sbjct: 310 TLEWAMSNLLNHPSILKKAKAELDAQVGQQHLVEESDLSKLNYLQNIISETLRLYPAVPL 369

Query: 302 LVPHYATNDCKICGYDVPRDTMVLVNAWAIHRDSNEWEDCMSFKPERYEKSEAVEIHKFL 361
           LVPHY++ DC + GYDVPR T++ +NAWAIHRD   WED  SFKPER+E  E+ E +K  
Sbjct: 370 LVPHYSSGDCTVGGYDVPRGTVLFINAWAIHRDPKLWEDAESFKPERFENGES-ESYKLF 429

Query: 362 PFGLGRRACPGSAMAHRVMGLTLATLIQCFEWKKIGEEDIDMKELSGAIMTKMVPLEAMC 421
           PFGLGRR+CPG  +A RV+GLTL +LIQCFEW++IGEE++DM E  G  M K VPLEA+C
Sbjct: 430 PFGLGRRSCPGIGLAQRVVGLTLGSLIQCFEWERIGEEEVDMAEGKGLTMPKAVPLEALC 489

Query: 422 KARPIIN 429
           KARPI+N
Sbjct: 490 KARPIMN 494

[Close]
BLAST of Cucsa.102780 vs. TAIR10
Match: AT4G37370.1 (AT4G37370.1 cytochrome P450, family 81, subfamily D, polypeptide 8)

HSP 1 Score: 525.0 bits (1351), Expect = 4.3e-149
Identity = 257/430 (59.77%), Postives = 334/430 (77.67%), Query Frame = 1 

Query: 2   SLRLGSKLAVVLSSSAAVEDCFTKNDVVLANRPRLLVGKYIGYNYTTMIGAPYGDHWRNL 61
           SLRLG++L  V SS +  E+CFTKNDVVLANRP  ++ K++ Y+YTTMI A YGDHWRNL
Sbjct: 69  SLRLGNRLVFVNSSHSIAEECFTKNDVVLANRPNFILAKHVAYDYTTMIAASYGDHWRNL 128

Query: 62  RRIGAIEIFSISRINKFTDIRRDEVMRIVRKLSHNSIHQFSKVEIQSAMSELTFNITMRM 121
           RRIG++EIFS  R+N F  IR+DE+ R+V +LS N   +F KV+++S +S+LTFN  +RM
Sbjct: 129 RRIGSVEIFSNHRLNSFLSIRKDEIRRLVFRLSRNFSQEFVKVDMKSMLSDLTFNNILRM 188

Query: 122 AAGKRYYGEDVTNEEEARRFRELIKEIVAVGGVSNPGDFLPVLNWMSKGIERKLIQLGKK 181
            AGKRYYG+ V ++ EA+R R+LI ++VA  G  N  D+LPVL  +S   E ++ +L  +
Sbjct: 189 VAGKRYYGDGVEDDPEAKRVRQLIADVVACAGAGNAVDYLPVLRLVSD-YETRVKKLAGR 248

Query: 182 VDAFLQGLIDDHRKTKEEGRNTMIDHLLSLQQSEPDHYNDQIIKGFILILLTAGSDTSAV 241
           +D FLQGL+D+ R+ KE+G NTMIDHLL+LQ+S+PD++ D+IIKG +L L+ AG+DTSAV
Sbjct: 249 LDEFLQGLVDEKREAKEKG-NTMIDHLLTLQESQPDYFTDRIIKGNMLALILAGTDTSAV 308

Query: 242 TMEWALSHLLNNPQVLKKAKEEIDTQIGQERLVEESDISKLPYLQRIVSETLRLNPAAPM 301
           T+EWALS++LN+P VL KA++EID +IG +RL++ESDIS LPYLQ IVSETLRL PAAPM
Sbjct: 309 TLEWALSNVLNHPDVLNKARDEIDRKIGLDRLMDESDISNLPYLQNIVSETLRLYPAAPM 368

Query: 302 LVPHYATNDCKICGYDVPRDTMVLVNAWAIHRDSNEWEDCMSFKPERYEKSEAVEIHKFL 361
           L+PH A+ DCK+ GYD+PR T++L N WAIHRD   W+D MSFKPER+EK    E  K +
Sbjct: 369 LLPHVASEDCKVAGYDMPRGTILLTNVWAIHRDPQLWDDPMSFKPERFEKEG--EAQKLM 428

Query: 362 PFGLGRRACPGSAMAHRVMGLTLATLIQCFEWKKIGEEDIDMKELSGAIMTKMVPLEAMC 421
           PFGLGRRACPGS +AHR++ LTL +LIQC EW+KIGEE +DM E  G  M K  PLEAMC
Sbjct: 429 PFGLGRRACPGSGLAHRLINLTLGSLIQCLEWEKIGEE-VDMSEGKGVTMPKAKPLEAMC 488

Query: 422 KARPIINNIF 432
           +ARP +  IF
Sbjct: 489 RARPSVVKIF 493

[Close]
BLAST of Cucsa.102780 vs. TAIR10
Match: AT4G37340.1 (AT4G37340.1 cytochrome P450, family 81, subfamily D, polypeptide 3)

HSP 1 Score: 510.4 bits (1313), Expect = 1.1e-144
Identity = 248/431 (57.54%), Postives = 326/431 (75.64%), Query Frame = 1 

Query: 1   MSLRLGSKLAVVLSSSAAVEDCFTKNDVVLANRPRLLVGKYIGYNYTTMIGAPYGDHWRN 60
           +SLRLG++L  V+SS +  E+CFTKNDVVLANR   L  K+I Y  TT++ A YGDHWRN
Sbjct: 68  ISLRLGNRLVFVVSSHSLAEECFTKNDVVLANRFNSLASKHISYGCTTVVTASYGDHWRN 127

Query: 61  LRRIGAIEIFSISRINKFTDIRRDEVMRIVRKLSHNSIHQFSKVEIQSAMSELTFNITMR 120
           LRRIGA+EIFS  R+N F+ IRRDE+ R++  LS NS  +F+KVE++S  S LTFN  +R
Sbjct: 128 LRRIGAVEIFSAHRLNSFSSIRRDEIHRLIACLSRNSSLEFTKVEMKSMFSNLTFNNIIR 187

Query: 121 MAAGKRYYGEDVTNEEEARRFRELIKEIVAVGGVSNPGDFLPVLNWMSKGIERKLIQLGK 180
           M AGK YYG+   ++ EA+R RELI E +   G  N  D+LP+L W++ G E+++ ++  
Sbjct: 188 MLAGKCYYGDGAEDDPEAKRVRELIAEGMGCFGAGNTADYLPILTWIT-GSEKRIKKIAS 247

Query: 181 KVDAFLQGLIDDHRKTKEEGRNTMIDHLLSLQQSEPDHYNDQIIKGFILILLTAGSDTSA 240
           ++D FLQGL+D+ R+ KE+ +NTM+DHLL LQ+++P++Y D IIKG +L L+ AG+DTSA
Sbjct: 248 RLDEFLQGLVDERREGKEKRQNTMVDHLLCLQETQPEYYTDNIIKGIMLSLILAGTDTSA 307

Query: 241 VTMEWALSHLLNNPQVLKKAKEEIDTQIGQERLVEESDISKLPYLQRIVSETLRLNPAAP 300
           VT+EW LS LLN+PQ+L KA++EID ++G  RLVEESD+S LPYLQ IVSE+LRL PA+P
Sbjct: 308 VTLEWTLSALLNHPQILSKARDEIDNKVGLNRLVEESDLSHLPYLQNIVSESLRLYPASP 367

Query: 301 MLVPHYATNDCKICGYDVPRDTMVLVNAWAIHRDSNEWEDCMSFKPERYEKSEAVEIHKF 360
           +LVPH A+ DCK+ GY +PR TM+L NAWAIHRD   W+D  SFKPER+EK    E  K 
Sbjct: 368 LLVPHVASEDCKVGGYHMPRGTMLLTNAWAIHRDPKIWDDPTSFKPERFEKEG--EAQKL 427

Query: 361 LPFGLGRRACPGSAMAHRVMGLTLATLIQCFEWKKIGEEDIDMKE-LSGAIMTKMVPLEA 420
           L FGLGRRACPGS +A R+  LT+ +LIQCFEW++IGEE++DM E   G IM K +PL A
Sbjct: 428 LGFGLGRRACPGSGLAQRLASLTIGSLIQCFEWERIGEEEVDMTEGGGGVIMPKAIPLVA 487

Query: 421 MCKARPIINNI 431
           MCKARP++  I
Sbjct: 488 MCKARPVVGKI 495

[Close]
BLAST of Cucsa.102780 vs. TAIR10
Match: AT4G37360.1 (AT4G37360.1 cytochrome P450, family 81, subfamily D, polypeptide 2)

HSP 1 Score: 498.4 bits (1282), Expect = 4.3e-141
Identity = 241/430 (56.05%), Postives = 323/430 (75.12%), Query Frame = 1 

Query: 1   MSLRLGSKLAVVLSSSAAVEDCFTKNDVVLANRPRLLVGKYIGYNYTTMIGAPYGDHWRN 60
           +SLRLG++L  V+SS +  E+CFTKNDV+LANR   +  K+I Y  +T++ A Y +HWRN
Sbjct: 68  ISLRLGNRLLFVVSSHSIAEECFTKNDVILANRQTTISTKHISYGNSTVVSASYSEHWRN 127

Query: 61  LRRIGAIEIFSISRINKFTDIRRDEVMRIVRKLSHNSIHQFSKVEIQSAMSELTFNITMR 120
           LRRIGA+EIFS  R+N F+ IRRDE+ R++ +L  NS + F+KVE++S  S+LTFN  +R
Sbjct: 128 LRRIGALEIFSAHRLNSFSSIRRDEIRRLIGRLLRNSSYGFTKVEMKSMFSDLTFNNIIR 187

Query: 121 MAAGKRYYGEDVTNEEEARRFRELIKEIVAVGGVSNPGDFLPVLNWMSKGIERKLIQLGK 180
           M AGK YYG+   ++ EA+R R LI E ++  G  N  D++P+L W++   E ++ +L  
Sbjct: 188 MLAGKCYYGDGKEDDPEAKRVRTLIAEAMSSSGPGNAADYIPILTWITYS-ETRIKKLAG 247

Query: 181 KVDAFLQGLIDDHRKTKEEGRNTMIDHLLSLQQSEPDHYNDQIIKGFILILLTAGSDTSA 240
           ++D FLQGL+D+ R+ KE+  NTM+DHLL LQ+++P++Y D+IIKG +L L+  G+DT+A
Sbjct: 248 RLDEFLQGLVDEKREGKEKKENTMVDHLLCLQETQPEYYMDRIIKGTMLSLIAGGTDTTA 307

Query: 241 VTMEWALSHLLNNPQVLKKAKEEIDTQIGQERLVEESDISKLPYLQRIVSETLRLNPAAP 300
           VT+EWALS LLNNP+VL KA++EID  IG +RL+EESDI  LPYLQ IVSETLRL PAAP
Sbjct: 308 VTLEWALSSLLNNPEVLNKARDEIDRMIGVDRLLEESDIPNLPYLQNIVSETLRLYPAAP 367

Query: 301 MLVPHYATNDCKICGYDVPRDTMVLVNAWAIHRDSNEWEDCMSFKPERYEKSEAVEIHKF 360
           ML+PH A+ DCK+ GYD+PR TM+L NAWAIHRD   W+D  SFKPER+EK    E  K 
Sbjct: 368 MLLPHVASKDCKVGGYDMPRGTMLLTNAWAIHRDPLLWDDPTSFKPERFEKEG--EAKKL 427

Query: 361 LPFGLGRRACPGSAMAHRVMGLTLATLIQCFEWKKIGEEDIDMKELSGAIMTKMVPLEAM 420
           +PFGLGRRACPGS +A R++ L+L +LIQCFEW++IGEE++DM E  G  M K  PLEAM
Sbjct: 428 MPFGLGRRACPGSGLAQRLVTLSLGSLIQCFEWERIGEEEVDMTEGPGLTMPKARPLEAM 487

Query: 421 CKARPIINNI 431
           C+AR  +  I
Sbjct: 488 CRARDFVGKI 494

[Close]
BLAST of Cucsa.102780 vs. TAIR10
Match: AT3G28740.1 (AT3G28740.1 Cytochrome P450 superfamily protein)

HSP 1 Score: 498.4 bits (1282), Expect = 4.3e-141
Identity = 247/430 (57.44%), Postives = 320/430 (74.42%), Query Frame = 1 

Query: 2   SLRLGSKLAVVLSSSAAVEDCFTKNDVVLANRPRLLVGKYIGYNYTTMIGAPYGDHWRNL 61
           SL LGS+L  V+SS A  E+CFTKNDVVLANRP  LVGK+IGYN TTM+GA YGD WRNL
Sbjct: 78  SLSLGSRLVFVVSSHAVAEECFTKNDVVLANRPEFLVGKHIGYNSTTMVGAAYGDSWRNL 137

Query: 62  RRIGAIEIFSISRINKFTDIRRDEVMRIVRKLSHNSIHQFSKVEIQSAMSELTFNITMRM 121
           RRIG IEIFS  R+N F  IR+DE+ R++  L+ NS H F KVE++     LT N  +RM
Sbjct: 138 RRIGTIEIFSSLRLNSFVSIRQDEIRRLIICLAKNSQHGFVKVEMKPLFMCLTINNIIRM 197

Query: 122 AAGKRYYGEDVTNEEEARRFRELIKEIVAVGGVSNPGDFLPVLNWMSKGIERKLIQLGKK 181
            AGKR+YG+   N+ EA+  R+LI E+V  GG  N  D+ P+L +++   E+ + +L  +
Sbjct: 198 VAGKRFYGDGTENDNEAKHVRQLIAEVVVSGGAGNAADYFPILRYVTN-YEKHVKKLAGR 257

Query: 182 VDAFLQGLIDDHRKTKEEGRNTMIDHLLSLQQSEPDHYNDQIIKGFILILLTAGSDTSAV 241
           VD FLQ L+++ R  K +G NTMIDHLLSLQ+++PD+Y D IIKG IL+++ AG+DTSA 
Sbjct: 258 VDEFLQSLVNEKRVEKVKG-NTMIDHLLSLQETQPDYYTDVIIKGIILVMILAGTDTSAG 317

Query: 242 TMEWALSHLLNNPQVLKKAKEEIDTQIGQERLVEESDISKLPYLQRIVSETLRLNPAAPM 301
           T+EWA+S+LLN+P+VL+KAK EID QIG +RLVEE DI KLPYLQ IVSETLRL P APM
Sbjct: 318 TLEWAMSNLLNHPEVLRKAKTEIDDQIGVDRLVEEQDIVKLPYLQHIVSETLRLYPVAPM 377

Query: 302 LVPHYATNDCKICGYDVPRDTMVLVNAWAIHRDSNEWEDCMSFKPERYEKSEAVEIHKFL 361
           L+PH A+ DC + GYDVPR T++LVNAWAIHRD   WE+   FKPER+EK    E  K +
Sbjct: 378 LLPHLASEDCIVDGYDVPRGTIILVNAWAIHRDPKLWEEPEKFKPERFEKKG--EDKKLM 437

Query: 362 PFGLGRRACPGSAMAHRVMGLTLATLIQCFEWKKIGEEDIDMKEL-SGAIMTKMVPLEAM 421
           PFG+GRR+CPGS +A R++ L L +L+QCFEW+++ E+ +DM+E   G  M K   L+AM
Sbjct: 438 PFGIGRRSCPGSGLAQRLVTLALGSLVQCFEWERVEEKYLDMRESEKGTTMRKATSLQAM 497

Query: 422 CKARPIINNI 431
           CKARPI++ +
Sbjct: 498 CKARPIVHKV 503

[Close]
BLAST of Cucsa.102780 vs. TAIR10
Match: AT5G36220.1 (AT5G36220.1 cytochrome p450 81d1)

HSP 1 Score: 498.4 bits (1282), Expect = 4.3e-141
Identity = 246/431 (57.08%), Postives = 328/431 (76.10%), Query Frame = 1 

Query: 1   MSLRLGSKLAVVLSS-SAAVEDCFTKNDVVLANRPRLLVGKYIGYNYTTMIGAPYGDHWR 60
           MSLRLGS+L  V+SS   A E+CF KNDVVLANRP++++GK++GYN T MI APYGDHWR
Sbjct: 74  MSLRLGSRLVYVVSSHKVAAEECFGKNDVVLANRPQVIIGKHVGYNNTNMIAAPYGDHWR 133

Query: 61  NLRRIGAIEIFSISRINKFTDIRRDEVMRIVRKLSHNSIHQFSKVEIQSAMSELTFNITM 120
           NLRR+  IEIFS  R+N F  +R DEV R++ +LS  +  + + VE++  + +LTFN  M
Sbjct: 134 NLRRLCTIEIFSTHRLNCFLYVRTDEVRRLISRLSRLAGTKKTVVELKPMLMDLTFNNIM 193

Query: 121 RMAAGKRYYGEDVTNEEEARRFRELIKEIVAVGGVSNPGDFLPVLNWMSKGIERKLIQLG 180
           RM  GKRYYGE+ T+EEEA+R R+L+ ++ A     N  D++P+L   S   E ++ +LG
Sbjct: 194 RMMTGKRYYGEETTDEEEAKRVRKLVADVGANTSSGNAVDYVPILRLFSS-YENRVKKLG 253

Query: 181 KKVDAFLQGLIDDHRKTKEEGRNTMIDHLLSLQQSEPDHYNDQIIKGFILILLTAGSDTS 240
           ++ D FLQGLIDD R  +E G  TMIDHLL LQ+S+ ++Y DQIIKG ILI++ AG++TS
Sbjct: 254 EETDKFLQGLIDDKRGQQETG-TTMIDHLLVLQKSDIEYYTDQIIKGIILIMVIAGTNTS 313

Query: 241 AVTMEWALSHLLNNPQVLKKAKEEIDTQIGQERLVEESDISKLPYLQRIVSETLRLNPAA 300
           AVT+EWALS+LLN+P V+ KA++EID ++G +RL+EE+D+S+LPYL+ IV ETLRL+PA 
Sbjct: 314 AVTLEWALSNLLNHPDVISKARDEIDNRVGLDRLIEEADLSELPYLKNIVLETLRLHPAT 373

Query: 301 PMLVPHYATNDCKICGYDVPRDTMVLVNAWAIHRDSNEWEDCMSFKPERYEKSEAVEIHK 360
           P+LVPH A+ DCKI  YD+PR T +LVNAWAIHRD N W+D  SFKPER+EK E  E  K
Sbjct: 374 PLLVPHMASEDCKIGSYDMPRGTTLLVNAWAIHRDPNTWDDPDSFKPERFEKEE--EAQK 433

Query: 361 FLPFGLGRRACPGSAMAHRVMGLTLATLIQCFEWKKIGEEDIDMKELSGAIMTKMVPLEA 420
            L FGLGRRACPGS +A R++GL L +LIQCFEW+++G  ++DMKE  G  + K +PL+A
Sbjct: 434 LLAFGLGRRACPGSGLAQRIVGLALGSLIQCFEWERVGNVEVDMKEGVGNTVPKAIPLKA 493

Query: 421 MCKARPIINNI 431
           +CKARP ++ I
Sbjct: 494 ICKARPFLHKI 500

[Close]
BLAST of Cucsa.102780 vs. NCBI nr
Match: gi|778718524|ref|XP_004141996.2| (PREDICTED: cytochrome P450 81E8-like [Cucumis sativus])

HSP 1 Score: 865.9 bits (2236), Expect = 2.9e-248
Identity = 432/432 (100.00%), Postives = 432/432 (100.00%), Query Frame = 1 

Query: 1   MSLRLGSKLAVVLSSSAAVEDCFTKNDVVLANRPRLLVGKYIGYNYTTMIGAPYGDHWRN 60
           MSLRLGSKLAVVLSSSAAVEDCFTKNDVVLANRPRLLVGKYIGYNYTTMIGAPYGDHWRN
Sbjct: 69  MSLRLGSKLAVVLSSSAAVEDCFTKNDVVLANRPRLLVGKYIGYNYTTMIGAPYGDHWRN 128

Query: 61  LRRIGAIEIFSISRINKFTDIRRDEVMRIVRKLSHNSIHQFSKVEIQSAMSELTFNITMR 120
           LRRIGAIEIFSISRINKFTDIRRDEVMRIVRKLSHNSIHQFSKVEIQSAMSELTFNITMR
Sbjct: 129 LRRIGAIEIFSISRINKFTDIRRDEVMRIVRKLSHNSIHQFSKVEIQSAMSELTFNITMR 188

Query: 121 MAAGKRYYGEDVTNEEEARRFRELIKEIVAVGGVSNPGDFLPVLNWMSKGIERKLIQLGK 180
           MAAGKRYYGEDVTNEEEARRFRELIKEIVAVGGVSNPGDFLPVLNWMSKGIERKLIQLGK
Sbjct: 189 MAAGKRYYGEDVTNEEEARRFRELIKEIVAVGGVSNPGDFLPVLNWMSKGIERKLIQLGK 248

Query: 181 KVDAFLQGLIDDHRKTKEEGRNTMIDHLLSLQQSEPDHYNDQIIKGFILILLTAGSDTSA 240
           KVDAFLQGLIDDHRKTKEEGRNTMIDHLLSLQQSEPDHYNDQIIKGFILILLTAGSDTSA
Sbjct: 249 KVDAFLQGLIDDHRKTKEEGRNTMIDHLLSLQQSEPDHYNDQIIKGFILILLTAGSDTSA 308

Query: 241 VTMEWALSHLLNNPQVLKKAKEEIDTQIGQERLVEESDISKLPYLQRIVSETLRLNPAAP 300
           VTMEWALSHLLNNPQVLKKAKEEIDTQIGQERLVEESDISKLPYLQRIVSETLRLNPAAP
Sbjct: 309 VTMEWALSHLLNNPQVLKKAKEEIDTQIGQERLVEESDISKLPYLQRIVSETLRLNPAAP 368

Query: 301 MLVPHYATNDCKICGYDVPRDTMVLVNAWAIHRDSNEWEDCMSFKPERYEKSEAVEIHKF 360
           MLVPHYATNDCKICGYDVPRDTMVLVNAWAIHRDSNEWEDCMSFKPERYEKSEAVEIHKF
Sbjct: 369 MLVPHYATNDCKICGYDVPRDTMVLVNAWAIHRDSNEWEDCMSFKPERYEKSEAVEIHKF 428

Query: 361 LPFGLGRRACPGSAMAHRVMGLTLATLIQCFEWKKIGEEDIDMKELSGAIMTKMVPLEAM 420
           LPFGLGRRACPGSAMAHRVMGLTLATLIQCFEWKKIGEEDIDMKELSGAIMTKMVPLEAM
Sbjct: 429 LPFGLGRRACPGSAMAHRVMGLTLATLIQCFEWKKIGEEDIDMKELSGAIMTKMVPLEAM 488

Query: 421 CKARPIINNIFY 433
           CKARPIINNIFY
Sbjct: 489 CKARPIINNIFY 500

[Close]
BLAST of Cucsa.102780 vs. NCBI nr
Match: gi|659079577|ref|XP_008440331.1| (PREDICTED: isoflavone 2'-hydroxylase-like [Cucumis melo])

HSP 1 Score: 826.2 bits (2133), Expect = 2.6e-236
Identity = 405/432 (93.75%), Postives = 422/432 (97.69%), Query Frame = 1 

Query: 1   MSLRLGSKLAVVLSSSAAVEDCFTKNDVVLANRPRLLVGKYIGYNYTTMIGAPYGDHWRN 60
           MSLRLGSKLAVVLSSSAAVE+CFTKNDVVLANRPRLL+GKYIGYNYTTM+ APYGDHWRN
Sbjct: 69  MSLRLGSKLAVVLSSSAAVEECFTKNDVVLANRPRLLIGKYIGYNYTTMVAAPYGDHWRN 128

Query: 61  LRRIGAIEIFSISRINKFTDIRRDEVMRIVRKLSHNSIHQFSKVEIQSAMSELTFNITMR 120
           LRRIGAIEIFSISRINKFTDIRRDEVMRIVRKLSHNSIHQFSKVEIQSAMSELTFNITMR
Sbjct: 129 LRRIGAIEIFSISRINKFTDIRRDEVMRIVRKLSHNSIHQFSKVEIQSAMSELTFNITMR 188

Query: 121 MAAGKRYYGEDVTNEEEARRFRELIKEIVAVGGVSNPGDFLPVLNWMSKGIERKLIQLGK 180
           MAAGKRYYGEDVTNEEEARRFR LIKEIVAVGGVSNPGDFLPVLNWMSKGIERKLIQLGK
Sbjct: 189 MAAGKRYYGEDVTNEEEARRFRALIKEIVAVGGVSNPGDFLPVLNWMSKGIERKLIQLGK 248

Query: 181 KVDAFLQGLIDDHRKTKEEGRNTMIDHLLSLQQSEPDHYNDQIIKGFILILLTAGSDTSA 240
           KVDAFLQGLIDDHR TKEEGRNTMIDHLLSLQQSEPDHYNDQIIKGFIL+LLTAGSDTSA
Sbjct: 249 KVDAFLQGLIDDHRNTKEEGRNTMIDHLLSLQQSEPDHYNDQIIKGFILVLLTAGSDTSA 308

Query: 241 VTMEWALSHLLNNPQVLKKAKEEIDTQIGQERLVEESDISKLPYLQRIVSETLRLNPAAP 300
           VTMEWAL HLLNNPQVLK A++E+DTQIGQERLVEESDI KLPYLQRI+ ETLRLNPAAP
Sbjct: 309 VTMEWALCHLLNNPQVLKMARDELDTQIGQERLVEESDIPKLPYLQRIIYETLRLNPAAP 368

Query: 301 MLVPHYATNDCKICGYDVPRDTMVLVNAWAIHRDSNEWEDCMSFKPERYEKSEAVEIHKF 360
           MLVPHYA+NDCKICGYDVPR+TMVL+NAWAIHRDSNEWED MSFKPERYEKS+A+E+HKF
Sbjct: 369 MLVPHYASNDCKICGYDVPRNTMVLINAWAIHRDSNEWEDHMSFKPERYEKSKAMEVHKF 428

Query: 361 LPFGLGRRACPGSAMAHRVMGLTLATLIQCFEWKKIGEEDIDMKELSGAIMTKMVPLEAM 420
           LPFG+GRRACPGSAMAHRVMGLTLATLIQCFEWKKIGEEDIDM+ELSGA+MTKMVPLEAM
Sbjct: 429 LPFGVGRRACPGSAMAHRVMGLTLATLIQCFEWKKIGEEDIDMRELSGALMTKMVPLEAM 488

Query: 421 CKARPIINNIFY 433
           CK RPIINNI Y
Sbjct: 489 CKTRPIINNILY 500

[Close]
BLAST of Cucsa.102780 vs. NCBI nr
Match: gi|778718513|ref|XP_011657880.1| (PREDICTED: cytochrome P450 81E8 [Cucumis sativus])

HSP 1 Score: 647.9 bits (1670), Expect = 1.3e-182
Identity = 306/433 (70.67%), Postives = 380/433 (87.76%), Query Frame = 1 

Query: 1   MSLRLGSKLAVVLSSSAAVEDCFTKNDVVLANRPRLLVGKYIGYNYTTMIGAPYGDHWRN 60
           +SL  GS+L VV+SS  AVE+CFTKND+VLANRPRLLVGK++GYN+TTM+ +PYGDHWRN
Sbjct: 64  ISLWFGSRLVVVVSSLPAVEECFTKNDIVLANRPRLLVGKHVGYNHTTMVASPYGDHWRN 123

Query: 61  LRRIGAIEIFSISRINKFTDIRRDEVMRIVRKLSHNSIHQFSKVEIQSAMSELTFNITMR 120
           LRRIGAIEIFS SR+NKF   R+DEV R+++KLS NS++ FSKVE+QS++SELTFNI+MR
Sbjct: 124 LRRIGAIEIFSASRLNKFAGTRKDEVERLLKKLSRNSMYGFSKVEMQSSISELTFNISMR 183

Query: 121 MAAGKRYYGEDVTNEEEARRFRELIKEIVAVGGVSNPGDFLPVLNWMSKGIERKLIQLGK 180
           MAAGKRY+GE+V + EEAR+ RELIK+IV++GGVSNPGDF+P++NW+  G +RK+ ++ K
Sbjct: 184 MAAGKRYFGEEVRDVEEARQSRELIKQIVSMGGVSNPGDFIPMMNWIPNGFKRKVWRVAK 243

Query: 181 KVDAFLQGLIDDHRKTKEEGRNTMIDHLLSLQQSEPDHYNDQIIKGFILILLTAGSDTSA 240
           ++D FLQGLID+HR  KEE RNTMIDHLLSLQ++EP++Y DQIIKG IL+LL AG+DTSA
Sbjct: 244 RMDGFLQGLIDEHRSNKEEERNTMIDHLLSLQETEPEYYGDQIIKGIILVLLLAGTDTSA 303

Query: 241 VTMEWALSHLLNNPQVLKKAKEEIDTQIGQERLVEESDISKLPYLQRIVSETLRLNPAAP 300
           VT+EWAL+HLLNNP+VLKKA+EE+DTQIG++RLVEESD+SKLPYLQ I+SETLRLNPAAP
Sbjct: 304 VTIEWALAHLLNNPEVLKKAREELDTQIGEKRLVEESDVSKLPYLQGIISETLRLNPAAP 363

Query: 301 MLVPHYATNDCKICGYDVPRDTMVLVNAWAIHRDSNEWEDCMSFKPERYEKSEAVEIH-- 360
           MLVPH  +NDC I GY +PRDT+VLVNAWAIHRD ++WE+   FKPER++KSE+++ H  
Sbjct: 364 MLVPHLTSNDCTISGYKIPRDTIVLVNAWAIHRDPHQWEEPTLFKPERHQKSESIDHHIS 423

Query: 361 KFLPFGLGRRACPGSAMAHRVMGLTLATLIQCFEWKKIGEEDIDMKELSGAIMTKMVPLE 420
           K +PFG+GRRACPGS MA RV+GLTLA LIQC+EW++IG+E +DM E  G  M KMVPLE
Sbjct: 424 KLIPFGVGRRACPGSGMAQRVVGLTLAALIQCYEWERIGDEKVDMSEGRGVTMPKMVPLE 483

Query: 421 AMCKARPIINNIF 432
           AMCK RPII+NIF
Sbjct: 484 AMCKPRPIIHNIF 496

[Close]
BLAST of Cucsa.102780 vs. NCBI nr
Match: gi|659079573|ref|XP_008440328.1| (PREDICTED: isoflavone 2'-hydroxylase [Cucumis melo])

HSP 1 Score: 637.1 bits (1642), Expect = 2.2e-179
Identity = 305/434 (70.28%), Postives = 375/434 (86.41%), Query Frame = 1 

Query: 1   MSLRLGSKLAVVLSSSAAVEDCFTKNDVVLANRPRLLVGKYIGYNYTTMIGAPYGDHWRN 60
           +SL  GS+L VV+SS  AVE+CFTKND+VLANRPRLLVGK++GYN+TTM+ +PYGDHWRN
Sbjct: 64  ISLWFGSRLVVVVSSLPAVEECFTKNDIVLANRPRLLVGKHVGYNHTTMVASPYGDHWRN 123

Query: 61  LRRIGAIEIFSISRINKFTDIRRDEVMRIVRKLSHNSIHQFSKVEIQSAMSELTFNITMR 120
           LRRIGAIEIFS SR+NKF   R+DEV R+++KLS NS++ FSKVE+QSA++ELTFNI+MR
Sbjct: 124 LRRIGAIEIFSASRLNKFAGTRKDEVERLLKKLSRNSMNGFSKVEMQSAIAELTFNISMR 183

Query: 121 MAAGKRYYGEDVTNEEEARRFRELIKEIVAVGGVSNPGDFLPVLNWMSKGIERKLIQLGK 180
           MAAGKRY+GE+VT+ EEAR+ RELIK+IV++GGVSNPGDF+P++NW+  G  RK+ +L K
Sbjct: 184 MAAGKRYFGEEVTDVEEARQSRELIKQIVSLGGVSNPGDFIPLMNWIPNGFRRKVSRLAK 243

Query: 181 KVDAFLQGLIDDHRKTKEEGRNTMIDHLLSLQQSEPDHYNDQIIKGFILILLTAGSDTSA 240
           ++D FLQGLID+HR  K E RNTMIDHLLSLQ+SEP++Y DQIIKG IL+LL AG+DTSA
Sbjct: 244 RMDGFLQGLIDEHRSNKCEERNTMIDHLLSLQESEPEYYGDQIIKGIILVLLLAGTDTSA 303

Query: 241 VTMEWALSHLLNNPQVLKKAKEEIDTQIGQERLVEESDISKLPYLQRIVSETLRLNPAAP 300
           VT+EWAL+HLLNN +VLKKA+EE+DTQIG++RLVEESDI KLPYLQ I+SETLRLNPAAP
Sbjct: 304 VTIEWALAHLLNNLEVLKKAREELDTQIGEKRLVEESDILKLPYLQGIISETLRLNPAAP 363

Query: 301 MLVPHYATNDCKICGYDVPRDTMVLVNAWAIHRDSNEWEDCMSFKPERYEKSEAVEIH-- 360
           MLVPH  +NDC I GY +PRDT+VLVNAWAIHR+ N+WE+   FKPER++KSE+   H  
Sbjct: 364 MLVPHLTSNDCTISGYKIPRDTIVLVNAWAIHREPNQWEEPTLFKPERHQKSESSSHHQI 423

Query: 361 -KFLPFGLGRRACPGSAMAHRVMGLTLATLIQCFEWKKIGEEDIDMKELSGAIMTKMVPL 420
            K +PFG+GRRACPGS MA RV+GLTLA LIQC+EW++IGEE +DM E  G  M K++PL
Sbjct: 424 PKLIPFGIGRRACPGSGMAQRVVGLTLAALIQCYEWERIGEEKVDMAEGRGVTMPKLLPL 483

Query: 421 EAMCKARPIINNIF 432
           EAMCK RPII++IF
Sbjct: 484 EAMCKPRPIIHHIF 497

[Close]
BLAST of Cucsa.102780 vs. NCBI nr
Match: gi|778718517|ref|XP_004141998.2| (PREDICTED: cytochrome P450 81E8-like [Cucumis sativus])

HSP 1 Score: 636.3 bits (1640), Expect = 3.8e-179
Identity = 299/434 (68.89%), Postives = 378/434 (87.10%), Query Frame = 1 

Query: 1   MSLRLGSKLAVVLSSSAAVEDCFTKNDVVLANRPRLLVGKYIGYNYTTMIGAPYGDHWRN 60
           +SLR GS+L VV+SS  AVE+CFTKND+VLANRPRLL GK+IGYN+TTM+G+PYGDHWRN
Sbjct: 64  ISLRFGSRLVVVVSSLPAVEECFTKNDIVLANRPRLLFGKHIGYNHTTMVGSPYGDHWRN 123

Query: 61  LRRIGAIEIFSISRINKFTDIRRDEVMRIVRKLSHNSIHQFSKVEIQSAMSELTFNITMR 120
           LRRIGAIEIFS SR+NKF   R+DEV R+++KLS NS+H +SKVE+QS++SELTFNI+MR
Sbjct: 124 LRRIGAIEIFSASRLNKFAGTRKDEVERLLKKLSRNSMHGYSKVEMQSSISELTFNISMR 183

Query: 121 MAAGKRYYGEDVTNEEEARRFRELIKEIVAVGGVSNPGDFLPVLNWMSKGIERKLIQLGK 180
           MAAGKRY+G++V + EEAR+ RELIK+IV++GGVSNPGDF+P++NW+  G +RK+ +LGK
Sbjct: 184 MAAGKRYFGDEVRDVEEARQSRELIKQIVSMGGVSNPGDFIPMMNWVPNGYKRKVSRLGK 243

Query: 181 KVDAFLQGLIDDHRKTKEEGRNTMIDHLLSLQQSEPDHYNDQIIKGFILILLTAGSDTSA 240
           ++D FLQGLID+HR  KEE RNTMIDHLLSLQ++EP++Y D+IIKG +L+LL AG+DTSA
Sbjct: 244 RMDRFLQGLIDEHRSNKEEERNTMIDHLLSLQETEPEYYGDEIIKGIVLVLLLAGTDTSA 303

Query: 241 VTMEWALSHLLNNPQVLKKAKEEIDTQIGQERLVEESDISKLPYLQRIVSETLRLNPAAP 300
           VT+EWAL+HLLNNP+VLKKA+EE+D QIG+++L EE D+SKLPYLQ I+ ETLRLNPAAP
Sbjct: 304 VTIEWALAHLLNNPEVLKKAREELDNQIGEKQLAEELDVSKLPYLQGIIYETLRLNPAAP 363

Query: 301 MLVPHYATNDCKICGYDVPRDTMVLVNAWAIHRDSNEWEDCMSFKPERYEKSEAVEIH-- 360
           MLVPH  +N+C I  Y++PRDT+VLVNAWAIHRD ++WE+   FKPER++  E+ + H  
Sbjct: 364 MLVPHLTSNNCTISEYEIPRDTIVLVNAWAIHRDPSQWEEPTLFKPERHQNLESSDHHQI 423

Query: 361 -KFLPFGLGRRACPGSAMAHRVMGLTLATLIQCFEWKKIGEEDIDMKELSGAIMTKMVPL 420
            K +PFG+GRRACPGS+MA RV+GLTLATLIQC+EW++IGEE +DM E  G  M K+VPL
Sbjct: 424 PKMIPFGVGRRACPGSSMAQRVVGLTLATLIQCYEWERIGEEKVDMTEGRGVTMPKVVPL 483

Query: 421 EAMCKARPIINNIF 432
           EAMCKARPI++NIF
Sbjct: 484 EAMCKARPIVHNIF 497

The following BLAST results are available for this feature:
BLAST of Cucsa.102780 vs. Swiss-Prot
Match NameE-valueIdentityDescription
C81E8_MEDTR8.8e-14458.16Cytochrome P450 81E8 OS=Medicago truncatula GN=CYP81E8 PE=2 SV=1[more]
C8D11_ARATH7.7e-14057.44Cytochrome P450 81D11 OS=Arabidopsis thaliana GN=CYP81D11 PE=2 SV=1[more]
C81D1_ARATH7.7e-14057.08Cytochrome P450 81D1 OS=Arabidopsis thaliana GN=CYP81D1 PE=2 SV=1[more]
C81E1_GLYEC2.5e-13853.81Isoflavone 2'-hydroxylase OS=Glycyrrhiza echinata GN=CYP81E1 PE=1 SV=2[more]
C81E9_MEDTR5.5e-13853.90Isoflavone 3'-hydroxylase (Fragment) OS=Medicago truncatula GN=CYP81E9 PE=1 SV=1[more]
BLAST of Cucsa.102780 vs. TrEMBL
Match NameE-valueIdentityDescription
A0A0A0KFV2_CUCSA2.0e-248100.00Uncharacterized protein OS=Cucumis sativus GN=Csa_6G492250 PE=3 SV=1[more]
A0A0A0KJM0_CUCSA8.8e-18370.67Cytochrome P450 OS=Cucumis sativus GN=Csa_6G492230 PE=3 SV=1[more]
A0A0A0KLF2_CUCSA2.6e-17968.89Uncharacterized protein OS=Cucumis sativus GN=Csa_6G492220 PE=3 SV=1[more]
A0A061EFX4_THECC2.1e-16064.90Cytochrome P450, family 81, subfamily D, polypeptide 8, putative OS=Theobroma ca... [more]
W9R984_9ROSA5.2e-15962.06Putative receptor-like protein kinase OS=Morus notabilis GN=L484_009298 PE=4 SV=... [more]
BLAST of Cucsa.102780 vs. TAIR10
Match NameE-valueIdentityDescription
AT4G37370.14.3e-14959.77 cytochrome P450, family 81, subfamily D, polypeptide 8[more]
AT4G37340.11.1e-14457.54 cytochrome P450, family 81, subfamily D, polypeptide 3[more]
AT4G37360.14.3e-14156.05 cytochrome P450, family 81, subfamily D, polypeptide 2[more]
AT3G28740.14.3e-14157.44 Cytochrome P450 superfamily protein[more]
AT5G36220.14.3e-14157.08 cytochrome p450 81d1[more]
BLAST of Cucsa.102780 vs. NCBI nr
Match NameE-valueIdentityDescription
gi|778718524|ref|XP_004141996.2|2.9e-248100.00PREDICTED: cytochrome P450 81E8-like [Cucumis sativus][more]
gi|659079577|ref|XP_008440331.1|2.6e-23693.75PREDICTED: isoflavone 2'-hydroxylase-like [Cucumis melo][more]
gi|778718513|ref|XP_011657880.1|1.3e-18270.67PREDICTED: cytochrome P450 81E8 [Cucumis sativus][more]
gi|659079573|ref|XP_008440328.1|2.2e-17970.28PREDICTED: isoflavone 2'-hydroxylase [Cucumis melo][more]
gi|778718517|ref|XP_004141998.2|3.8e-17968.89PREDICTED: cytochrome P450 81E8-like [Cucumis sativus][more]
Annotated Terms
The following terms have been associated with this gene:
Vocabulary: INTERPRO
TermDefinition
IPR001128Cyt_P450
IPR002401Cyt_P450_E_grp-I
IPR017972Cyt_P450_CS
Vocabulary: Molecular Function
TermDefinition
GO:0016705oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen
GO:0005506iron ion binding
GO:0020037heme binding
Vocabulary: Biological Process
TermDefinition
GO:0055114oxidation-reduction process
GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0055114 oxidation-reduction process
biological_process GO:0006468 protein phosphorylation
cellular_component GO:0005575 cellular_component
molecular_function GO:0005524 ATP binding
molecular_function GO:0020037 heme binding
molecular_function GO:0005506 iron ion binding
molecular_function GO:0004497 monooxygenase activity
molecular_function GO:0016705 oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen
molecular_function GO:0004672 protein kinase activity
Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Cucsa.102780.1Cucsa.102780.1mRNA


InterPro
Analysis Name: InterPro Annotations of cucumber (Gy14)
Date Performed: 2017-01-17
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001128Cytochrome P450PRINTSPR00385P450coord: 287..298
score: 2.9E-11coord: 370..381
score: 2.9E-11coord: 361..370
score: 2.9E-11coord: 234..251
score: 2.9
IPR001128Cytochrome P450GENE3DG3DSA:1.10.630.10coord: 1..424
score: 3.6E
IPR001128Cytochrome P450PFAMPF00067p450coord: 2..413
score: 2.5
IPR001128Cytochrome P450unknownSSF48264Cytochrome P450coord: 2..424
score: 1.7E
IPR002401Cytochrome P450, E-class, group IPRINTSPR00463EP450Icoord: 243..269
score: 1.0E-36coord: 327..351
score: 1.0E-36coord: 18..39
score: 1.0E-36coord: 223..240
score: 1.0E-36coord: 286..304
score: 1.0E-36coord: 370..393
score: 1.0E-36coord: 113..131
score: 1.0E-36coord: 360..370
score: 1.0
IPR017972Cytochrome P450, conserved sitePROSITEPS00086CYTOCHROME_P450coord: 363..372
scor
NoneNo IPR availablePANTHERPTHR24298FAMILY NOT NAMEDcoord: 1..428
score: 3.9E
NoneNo IPR availablePANTHERPTHR24298:SF61CYTOCHROME P450 81D1-RELATEDcoord: 1..428
score: 3.9E

Synteny
The following gene(s) are orthologous to this gene:
GeneOrthologueOrganismBlock
Cucsa.102780CmaCh20G009770Cucurbita maxima (Rimu)cgycmaB0227
The following gene(s) are paralogous to this gene:

None

The following block(s) are covering this gene:
GeneOrganismBlock
Cucsa.102780Cucurbita pepo (Zucchini)cgycpeB0211
Cucsa.102780Cucurbita pepo (Zucchini)cgycpeB0213
Cucsa.102780Cucurbita pepo (Zucchini)cgycpeB0215
Cucsa.102780Cucurbita pepo (Zucchini)cgycpeB0217
Cucsa.102780Cucurbita pepo (Zucchini)cgycpeB0218
Cucsa.102780Cucurbita pepo (Zucchini)cgycpeB0220
Cucsa.102780Cucurbita pepo (Zucchini)cgycpeB0223
Cucsa.102780Cucurbita pepo (Zucchini)cgycpeB0227
Cucsa.102780Bottle gourd (USVL1VR-Ls)cgylsiB125
Cucsa.102780Bottle gourd (USVL1VR-Ls)cgylsiB129
Cucsa.102780Melon (DHL92) v3.6.1cgymedB124
Cucsa.102780Melon (DHL92) v3.6.1cgymedB125
Cucsa.102780Melon (DHL92) v3.6.1cgymedB131
Cucsa.102780Silver-seed gourdcarcgyB0219
Cucsa.102780Silver-seed gourdcarcgyB0433
Cucsa.102780Silver-seed gourdcarcgyB0467
Cucsa.102780Silver-seed gourdcarcgyB0918
Cucsa.102780Cucumber (Chinese Long) v3cgycucB117
Cucsa.102780Cucumber (Chinese Long) v3cgycucB123
Cucsa.102780Cucumber (Chinese Long) v3cgycucB126
Cucsa.102780Cucumber (Chinese Long) v3cgycucB127
Cucsa.102780Watermelon (97103) v2cgywmbB127
Cucsa.102780Watermelon (97103) v2cgywmbB133
Cucsa.102780Wax gourdcgywgoB146
Cucsa.102780Wax gourdcgywgoB151
Cucsa.102780Cucumber (Gy14) v1cgycgyB044
Cucsa.102780Cucumber (Gy14) v1cgycgyB052
Cucsa.102780Cucurbita maxima (Rimu)cgycmaB0228
Cucsa.102780Cucurbita maxima (Rimu)cgycmaB0226
Cucsa.102780Cucurbita maxima (Rimu)cgycmaB0221
Cucsa.102780Cucurbita maxima (Rimu)cgycmaB0231
Cucsa.102780Cucurbita maxima (Rimu)cgycmaB0232
Cucsa.102780Cucurbita maxima (Rimu)cgycmaB0234
Cucsa.102780Cucurbita maxima (Rimu)cgycmaB0235
Cucsa.102780Cucurbita moschata (Rifu)cgycmoB0215
Cucsa.102780Cucurbita moschata (Rifu)cgycmoB0219
Cucsa.102780Cucurbita moschata (Rifu)cgycmoB0222
Cucsa.102780Cucurbita moschata (Rifu)cgycmoB0224
Cucsa.102780Cucurbita moschata (Rifu)cgycmoB0227
Cucsa.102780Cucurbita moschata (Rifu)cgycmoB0228
Cucsa.102780Cucurbita moschata (Rifu)cgycmoB0231
Cucsa.102780Cucurbita moschata (Rifu)cgycmoB0233
Cucsa.102780Wild cucumber (PI 183967)cgycpiB115
Cucsa.102780Wild cucumber (PI 183967)cgycpiB121
Cucsa.102780Wild cucumber (PI 183967)cgycpiB122
Cucsa.102780Wild cucumber (PI 183967)cgycpiB123
Cucsa.102780Cucumber (Chinese Long) v2cgycuB113
Cucsa.102780Cucumber (Chinese Long) v2cgycuB120
Cucsa.102780Cucumber (Chinese Long) v2cgycuB121
Cucsa.102780Cucumber (Chinese Long) v2cgycuB122
Cucsa.102780Melon (DHL92) v3.5.1cgymeB125
Cucsa.102780Melon (DHL92) v3.5.1cgymeB126
Cucsa.102780Melon (DHL92) v3.5.1cgymeB130
Cucsa.102780Melon (DHL92) v3.5.1cgymeB131
Cucsa.102780Watermelon (Charleston Gray)cgywcgB134
Cucsa.102780Watermelon (Charleston Gray)cgywcgB137
Cucsa.102780Watermelon (Charleston Gray)cgywcgB138
Cucsa.102780Watermelon (97103) v1cgywmB132
Cucsa.102780Watermelon (97103) v1cgywmB136