Cucsa.101350 (gene) Cucumber (Gy14) v1

BLAST of Cucsa.101350 vs. Swiss-Prot
Match: IRE1A_ARATH (Serine/threonine-protein kinase/endoribonuclease IRE1a OS=Arabidopsis thaliana GN=IRE1A PE=1 SV=1)

HSP 1 Score: 660.2 bits (1702), Expect = 3.3e-188
Identity = 330/522 (63.22%), Postives = 412/522 (78.93%), Query Frame = 1

Query: 420 ITMFLGLINRGGALVAKLKQFLLKEKQPSAVSSKIVSSKKKKARKLGKNGNFDKKDASAS 479
           + +   L+  G  +   +K+F     + S VS K   SKKKK RK  K+ N  ++     
Sbjct: 327 VKLLAPLVLLGVVVSVFIKKF---SSRGSDVSLKAGPSKKKKNRKSAKDTN--RQSVPRG 386

Query: 480 SENEDMVRSEGDFNNWFPPNNLIDTSGNGRQIGKLMVTNKEIAKGSNGTIVLEGVYEGRL 539
            +  +++   G     F  NN    + +GR+IGKL +++KEIAKGSNGT+V EG+YEGR 
Sbjct: 387 QDQFELIEG-GQMLLGF--NNFQSGATDGRKIGKLFLSSKEIAKGSNGTVVFEGIYEGRP 446

Query: 540 VAVKRLVKTHHDVAFKEVQNLIASDRHQNIVRWYGVEYDQDFVYLSLERCTCSLDDLIQI 599
           VAVKRLV++HH+VAFKE+QNLIASD+H NI+RWYGVEYDQDFVYLSLERCTCSLDDLI+ 
Sbjct: 447 VAVKRLVRSHHEVAFKEIQNLIASDQHTNIIRWYGVEYDQDFVYLSLERCTCSLDDLIKS 506

Query: 600 CSDPSLNSLLSLDEDAGPMIDYKLRLESLKNVISDLNLWKKNSRPAPLLLGLLRDMVAGL 659
             + S+  +L  ++    +  YK++L+SL+ VI   N WK    P+PL+L L+RD+V G+
Sbjct: 507 YLEFSMTKVLENNDSTEGVAAYKIQLDSLEGVIKGNNFWKVGGHPSPLMLKLMRDIVCGI 566

Query: 660 EHLHELGIIHRDLKPQNVLITKQKSVRAKLSDMGISKRLLKDMSSLGHHATGCGSSGWQA 719
            HLHELGI+HRDLKPQNVLI+K  ++ AKLSDMGISKR+ +DMSSLGH ATG GSSGWQA
Sbjct: 567 VHLHELGIVHRDLKPQNVLISKDMTLSAKLSDMGISKRMSRDMSSLGHLATGSGSSGWQA 626

Query: 720 PEQLLHGRQTRAIDLFSLGCVIFFCLTGGRHPFGDHFERDVNIVRNQMDLFLVEGIPEAM 779
           PEQLL GRQTRA+D+FSLGCVIF+ +TG +HPFGD  ERDVNIV+N++DLFLVE +PEA 
Sbjct: 627 PEQLLQGRQTRAVDMFSLGCVIFYTITGCKHPFGDDLERDVNIVKNKVDLFLVEHVPEAS 686

Query: 780 DLISQLLNPNPDLRPRASVVLQHPLFWSSEVRLSFLRDTSDRVELEDRETHSDLLEALES 839
           DLIS+LLNP+PDLRP A+ VL HP+FW+SE+RLSFLRD SDRVELE+RE  S++L+A+ES
Sbjct: 687 DLISRLLNPDPDLRPSATEVLLHPMFWNSEMRLSFLRDASDRVELENREADSEILKAMES 746

Query: 840 TAPLALGGKWDEKLDPAFITNIGQYRRYKYDSVRDLLRVMRNKLNHYRELPKEIQELIGS 899
           TAP+A+GGKWDEKL+P FITNIG+YRRYKYDS+RDLLRV+RNKLNH+RELP EIQEL+G+
Sbjct: 747 TAPVAIGGKWDEKLEPVFITNIGRYRRYKYDSIRDLLRVIRNKLNHHRELPPEIQELVGT 806

Query: 900 VPEGFDNYFASRFPRLLTEVYRVISQYCREEEGFWKYFKSHV 942
           VPEGFD YFA RFP+LL EVYRVIS +CREEE F KYFK  +
Sbjct: 807 VPEGFDEYFAVRFPKLLIEVYRVISLHCREEEVFRKYFKCDI 840

HSP 2 Score: 78.2 bits (191), Expect = 5.4e-13
Identity = 71/265 (26.79%), Postives = 115/265 (43.40%), Query Frame = 1

Query: 2   RCRFFRRLWLFLLLISRFRSLTAESRTYDNLKFSQLGSYGENSDELAQIRGKSLLPLSLK 61
           RC F R L+  LLL+S +          D        S G  S  + QIR +        
Sbjct: 4   RCPFLRHLFFLLLLLSPWIMSPCGGAADDVTYPIVPSSPGRRS--ILQIRREP------P 63

Query: 62  SKGNTALIAASGGDIYLVDSDSKKIIWSFASGTPIYSAYQSPTNYNKENASGSTRSPFFF 121
           ++ NT L+    G ++L     +   WSF++G+P++S YQ+P N N ENA+  TR     
Sbjct: 64  TEPNTKLVVDRDGKVFLKQQPKETPYWSFSTGSPMHSLYQAPANNNTENATEITRPHIIV 123

Query: 122 DCGDDWELYIHTEHGRTKLPR----TIDEVVRSTPYIFEDGSVMTGSRKTTVYEVNPVTG 181
           +       Y++     T +      T+ E  R  P + +DG V  GS  T+ Y V+  +G
Sbjct: 124 E-------YLNNSKAATTVDGYHNWTVQEFFRQKPLVTDDG-VTLGSETTSAYLVDGRSG 183

Query: 182 KLIRNHSSELSPSGLSNDEFSVLNGNSSTNKLENRDLIQPGLMKP-IEQRLYITRTDYFL 241
           +LI  + S                  +   K+ N  L++P   +  + + L I RTD  L
Sbjct: 184 RLIHVYKS------------------TGDTKITNA-LVKPASTEDFVNEPLLIRRTDSKL 233

Query: 242 KSSFAGSEEVSWSLNVADIGATLVC 262
           +     + ++ W+L V+   A L+C
Sbjct: 244 EHFSKTTGKLVWNLTVSHFRAALLC 233

BLAST of Cucsa.101350 vs. Swiss-Prot
Match: IRE1B_ARATH (Serine/threonine-protein kinase/endoribonuclease IRE1b OS=Arabidopsis thaliana GN=IRE1B PE=2 SV=1)

HSP 1 Score: 592.4 bits (1526), Expect = 8.6e-168
Identity = 310/570 (54.39%), Postives = 406/570 (71.23%), Query Frame = 1

Query: 378 IPMPLMKINDSSAVLGHNIGTTNVDFIAMVLNGPLGLFIALFITMFLGLINRGGALVAKL 437
           +P+P   I+  + ++    G+T     A   +G + L     +TM    ++  G    +L
Sbjct: 329 LPLPETVISQITDIID---GSTKQAGFASKFSGLIVLIFGFCVTM----LSVCGLFFYRL 388

Query: 438 KQFLLKEKQPSAVSSKIVSSKKKKARKLGKNGNFDKKDAS-ASSENEDMVRSEGDFNNWF 497
           +Q + + K+P      I + KKKK++K G      KK+    S  N+D    E +     
Sbjct: 389 RQSI-RIKEPYVSEVPIATPKKKKSKKNGTTKAVHKKENGFISGGNKDPSHEENEKRLLT 448

Query: 498 PPNNLIDTSGNGRQIGKLMVTNKEIAKGSNGTIVLEGVYEGRLVAVKRLVKTHHDVAFKE 557
               L ++S  G ++GKL V+NKEIAKGSNGT+VLEG YEGRLVAVKRLV++HHDVA KE
Sbjct: 449 AFPGLNNSSAEGYRVGKLFVSNKEIAKGSNGTVVLEGSYEGRLVAVKRLVQSHHDVAQKE 508

Query: 558 VQNLIASDRHQNIVRWYGVEYDQDFVYLSLERCTCSLDDLIQICSDPSLNSLLSLDEDAG 617
           + NL+ASD+H NIVRWYGV+ D+ F+Y+SLE C CSL+DLI   S     +LL       
Sbjct: 509 ILNLMASDKHSNIVRWYGVDQDEHFIYISLELCACSLNDLIYASS-----ALLE-----S 568

Query: 618 PMIDYKLRLESLKNVISD---LNLWKKNSRPAPLLLGLLRDMVAGLEHLHELGIIHRDLK 677
           PM    +    +  +  +   + LWK+N  P+P+LL L+RD+VAGL HLH++GI+HRDLK
Sbjct: 569 PMASSSIHSIQINPIFENGKGVELWKENGHPSPVLLKLMRDIVAGLVHLHDIGIVHRDLK 628

Query: 678 PQNVLITKQKSVRAKLSDMGISKRLLKDMSSLGHHATGC--GSSGWQAPEQLLHGRQTRA 737
           PQNVLI K  S+ AKLSDMGISKRL  D S+L  ++TG   GSSGWQAPEQL + RQTRA
Sbjct: 629 PQNVLIVKNSSLCAKLSDMGISKRLPADTSALTRNSTGLGSGSSGWQAPEQLRNERQTRA 688

Query: 738 IDLFSLGCVIFFCLTGGRHPFGDHFERDVNIVRNQMDLFLVEGIPEAMDLISQLLNPNPD 797
           +DLFSLGCV+FFC+TGG+HP+GD++ERDVN++ +Q DLFL+E +PEA+ L++ LLNP+P+
Sbjct: 689 VDLFSLGCVLFFCMTGGKHPYGDNYERDVNVLNDQKDLFLIESLPEAVHLLTGLLNPDPN 748

Query: 798 LRPRASVVLQHPLFWSSEVRLSFLRDTSDRVELEDRETHSDLLEALESTAPLALGGKWDE 857
           LRPRA  V+ HPLFW+S++RLSFLRD SDRVELE+RE  S LL ALESTA + L G+WDE
Sbjct: 749 LRPRAQDVMHHPLFWNSDMRLSFLRDASDRVELENREEGSQLLAALESTAAVTLNGRWDE 808

Query: 858 KLDPAFITNIGQYRRYKYDSVRDLLRVMRNKLNHYRELPKEIQELIGSVPEGFDNYFASR 917
           KLD  F+ NIG+YRRYK+DS+RDLLRV+RNKLNHYRELPKE+QEL+GSVPEGF+ YF+SR
Sbjct: 809 KLDSIFLDNIGRYRRYKFDSIRDLLRVIRNKLNHYRELPKELQELLGSVPEGFERYFSSR 868

Query: 918 FPRLLTEVYRVISQYCREEEGFWKYFKSHV 942
           FP+LL +VY V+  YC  EE F+KY K+ V
Sbjct: 869 FPKLLIQVYTVLFDYCNNEEFFFKYSKTTV 880

HSP 2 Score: 71.6 bits (174), Expect = 5.1e-11
Identity = 57/202 (28.22%), Postives = 97/202 (48.02%), Query Frame = 1

Query: 68  LIAASGGDIYLVDSDSKKIIWSFASGTPIYSAYQSPTNY--NKENASGSTRSPFFFDCGD 127
           L++   G I LVD  S+K+ W+F +  PIYS+YQ+P  +    E  S      F+ DC  
Sbjct: 50  LVSTVDGSISLVDMSSQKLDWTFHTNEPIYSSYQAPHYHYTTDEERSSVLGDDFYMDCDK 109

Query: 128 DWELYIHTEHGRTKLPRTID--EVVRSTPYIFEDGSVMTGSRKTTVYEVNPVTGKLIRNH 187
           DW LY  +     ++   +D  E + + PY   D  ++ G + T+V+ ++  TGKL++ +
Sbjct: 110 DWRLYNSSVRKGKRVNEIVDASEFIGTLPYTSTD-RIVLGKKDTSVFLLDWKTGKLVKRY 169

Query: 188 SSE--LSPSGLSNDEFSVLNGNSSTNKLENRDLIQPGLMK--PIEQRLYITRTDYFLK-- 247
             +   S + + ND+   +         E   L   G  K     + +YI R D+ ++  
Sbjct: 170 RMDELYSNTVVENDKEKAI-----VLSKEAPLLFGSGFKKSEDFPELVYIERKDFKIQCI 229

Query: 248 SSFAGSEEVSWSLNVADIGATL 260
           S F    +V WS++ A + A L
Sbjct: 230 SKFG---DVLWSVSYAKMEAKL 242

BLAST of Cucsa.101350 vs. Swiss-Prot
Match: IRE1L_ARATH (Inactive serine/threonine-protein kinase/endoribonuclease IRE1-like OS=Arabidopsis thaliana GN=At3g11870 PE=3 SV=1)

HSP 1 Score: 318.9 bits (816), Expect = 1.8e-85
Identity = 218/532 (40.98%), Postives = 300/532 (56.39%), Query Frame = 1

Query: 443 KEKQPSAVSSKIVSSKKKKA-RKLGKNGNFDKKDASASSENEDM-VRSEGDFNNWFP--- 502
           K   P   +S    +K ++  +K   +G   +++   +SE++ + V S+ + N   P   
Sbjct: 39  KNSAPRVSASGEDGTKNEQVEKKSDPSGGLGEENEKTNSESKVLSVPSDQNINKTLPVML 98

Query: 503 PNNLIDTSGNGRQIGK-----LMVTNKEIAKGSNGTIVLEGVYEGRL-VAVKRLVKTHHD 562
           P+  +         GK     L+V+  E+  G NG  V +GVY  R  VAVK L   H  
Sbjct: 99  PSLELRKYDENETPGKVVNRRLLVSTNEMKYGRNGYEVFQGVYGRRSSVAVKCLDLAHTT 158

Query: 563 VAF--KEVQNLIASDRHQNIVRWYGVEYDQDFVYLSLERCTCSLDDLIQICSDPSLNSLL 622
            AF   E+ N    D H NI+R++G+E DQ F Y+ LE   CSLDDLI++    S+    
Sbjct: 159 EAFIQNEIDNHCLCDDHSNIIRFHGLEQDQSFAYICLEPWKCSLDDLIKL----SVRRTK 218

Query: 623 SLDEDAGPMIDYKLRLESLKNVISDLNLWKKNSRPAPL--LLGLLRDMVAGLEHLHELGI 682
              +   P+ D       L+ V+  +  WK+  +P PL  +L L+RD+V GL HLH+L  
Sbjct: 219 RDTQAVAPVDD-------LEKVMKRIKFWKEKGKPLPLTPMLKLMRDVVCGLAHLHKLKT 278

Query: 683 IHRDLKPQNVLI-TKQKSVRAKLSDMGISKRLLKDMSSLGHHATGCGSSGWQAPEQLLHG 742
           IHR+L PQNVLI  K  ++ AK+SDM +SK L    SS  H AT  GSSGWQAPEQL   
Sbjct: 279 IHRNLNPQNVLIIVKDMTLTAKISDMSLSKHLGGKKSSYKHLATCSGSSGWQAPEQLNKD 338

Query: 743 RQTRA---IDLFSLGCVIFFCLTGGRHPFGDHFERDVNI-VRNQMDLFLVEGIPEAMDLI 802
           ++ +     D+F+ GC++ + + G  HPFG   ERD NI   N+ +L LV  + EA++LI
Sbjct: 339 KKKKEDFPADMFNFGCLLHYAVMG-THPFGSPSERDTNIKTNNKTNLSLVTNL-EAINLI 398

Query: 803 SQLLNPNPDLRPRASVVLQHPLFWSSEVRLSFLRDTSDRVELEDRETHSDLLEALESTAP 862
            QLLN  PDLRP A+ VL HPLFW SE RL FLR+ SDR+EL D     DL + +   AP
Sbjct: 399 EQLLNYKPDLRPSATQVLLHPLFWDSEKRLFFLREASDRIEL-DITMWGDLNKTI---AP 458

Query: 863 LALGGK--WDEKLDPAFITNI------------GQYRR-YKYDSVRDLLRVMRNKLNHYR 922
             LG    W  KL   FIT+I             QY R YKY S+R LLR++RN L+H+R
Sbjct: 459 RVLGESKDWASKLGKTFITHIENLAQAQPGQESRQYNRSYKYWSLRHLLRLIRNILSHHR 518

Query: 923 EL--PKEIQELIGSVPEGFDNYFASRFPRLLTEVYRVISQYCREEEGFWKYF 938
           E+    +I+E++G VPEG D +F +RFP L+ E+Y  IS +C+ EE F KYF
Sbjct: 519 EILDDPKIKEMVGKVPEGLDIFFTARFPNLMMEIYAFISMHCKGEEAFEKYF 553

BLAST of Cucsa.101350 vs. Swiss-Prot
Match: IREA_DICDI (Probable serine/threonine-protein kinase ireA OS=Dictyostelium discoideum GN=ireA PE=3 SV=1)

HSP 1 Score: 281.6 bits (719), Expect = 3.2e-74
Identity = 187/527 (35.48%), Postives = 271/527 (51.42%), Query Frame = 1

Query: 446 QPSAVSSKIVSSKKKKARKLGKNGNFDKKDASASSEN----------------------- 505
           Q + +++K    KKKK +K   N N + +D     EN                       
Sbjct: 482 QNNNINNKKTPKKKKKKQKNKNNKNNNDEDDENEIENYNDNQNDLIDEFISTNSVIQQQQ 541

Query: 506 ---EDMVRSEGDFNNWFPPNNLIDTSGNGRQIGKL-MVTNKEIAKGSNGTIVLEGVYEGR 565
              + ++ S    NN      +I  +GN + IGKL ++TNK +  GS GTIV EG  EGR
Sbjct: 542 QQQQQLINSSNKINNG--SGKIILDNGNVK-IGKLEIITNKILGTGSCGTIVYEGKMEGR 601

Query: 566 LVAVKRLVKTHHDVAFKEVQNLIASDRHQNIVRWYGVEYDQDFVYLSLERCTCSLDDLIQ 625
            VAVKR++      A +EV  LI SD H N+VR+Y  E D +F+YL++  C  SLD  +Q
Sbjct: 602 KVAVKRMLSQFVKFADREVSILIHSDEHTNVVRYYAKEEDDEFIYLAISFCQKSLDMYVQ 661

Query: 626 ICSDPSLNSLLSLDEDAGPMIDYKLRLESLKNVISDLNLWKKNSRPAPLLLGLLRDMVA- 685
                     L +     P I          N   + N    N+    ++    + M+  
Sbjct: 662 QTLS------LQISPTDSPSIQSS-------NNNGNGNNGNNNNNNQIIIDNKTKQMILE 721

Query: 686 ---GLEHLHELGIIHRDLKPQNVLITKQKSVRAKLSDMGISKRLLKDMSSLGHHATGCGS 745
              GLEHLH L I+HRD+KP NVLI      R K+SDMG+ K L  D  SL   +    S
Sbjct: 722 LFKGLEHLHSLNIVHRDIKPHNVLIDPNN--RVKISDMGLGKLLDNDDQSLTFTSD---S 781

Query: 746 SGWQAPEQLLHG--RQTRAIDLFSLGCVIFFCLTGGRHPFGDHFERDVNIVRNQMDLFLV 805
            GWQ P + L+G  R T+ +D+FSLGCV+++ LTG  HPFG  + R+ N+++ + D+  +
Sbjct: 782 HGWQ-PAEYLNGTNRNTKKVDIFSLGCVVYYLLTGA-HPFGHRYNREKNVLKGKFDIDQI 841

Query: 806 EGIPEAMDLISQLLNPNPDLRPRASVVLQHPLFWSSEVRLSFLRDTSDRVELEDRETHSD 865
           + +P+   L+  ++   P+ RP     + HP FW    +LSFL   SD +E E  +  S 
Sbjct: 842 KHLPDIHQLVHSMIQFEPEKRPDIGECINHPFFWEVHKKLSFLVAASDYLEFE--KPTSP 901

Query: 866 LLEALESTAPLALGGK--WDEKLDPAFITNIGQYRRYKYDSVRDLLRVMRNKLNHYRELP 925
           L   ++S   L   G   W  K+D   I NIG+YR+Y   S+RDLLRV+RNK NHYR+L 
Sbjct: 902 LNLEIDSHVDLVTDGSGDWWLKIDQVLIDNIGRYRKYNGKSIRDLLRVIRNKFNHYRDLS 961

Query: 926 KEIQELIGSVPEGFDNYFASRFPRLLTEVYRVISQYCREEEGFWKYF 938
            E Q  +G +P+GF NYF  +FP+L    Y  I +  + ++ F +Y+
Sbjct: 962 PEEQTCLGILPDGFFNYFDLKFPQLFIVTYLFILKNLKNDQYFVQYY 983

BLAST of Cucsa.101350 vs. Swiss-Prot
Match: ERN2_HUMAN (Serine/threonine-protein kinase/endoribonuclease IRE2 OS=Homo sapiens GN=ERN2 PE=1 SV=4)

HSP 1 Score: 262.7 bits (670), Expect = 1.6e-68
Identity = 166/438 (37.90%), Postives = 234/438 (53.42%), Query Frame = 1

Query: 511 IGKLMVTNKEI-AKGSNGTIVLEGVYEGRLVAVKRLVKTHHDVAFKEVQNLIASDRHQNI 570
           +GK+    K++  +G+ GT V  G +EGR VAVKRL++    +  +EVQ L  SDRH N+
Sbjct: 515 VGKISFNPKDVLGRGAGGTFVFRGQFEGRAVAVKRLLRECFGLVRREVQLLQESDRHPNV 574

Query: 571 VRWYGVEYDQDFVYLSLERCTCSLDDLIQICSDPSLNSLLSLDEDAGPMIDYKLRLESLK 630
           +R++  E    F Y++LE C  SL + ++           + D D G  ++ ++ L+ L 
Sbjct: 575 LRYFCTERGPQFHYIALELCRASLQEYVE-----------NPDLDRGG-LEPEVVLQQLM 634

Query: 631 NVISDLNLWKKNSRPAPLLLGLLRDMVAGLEHLHELGIIHRDLKPQNVLIT---KQKSVR 690
                                      +GL HLH L I+HRDLKP N+LIT    Q   R
Sbjct: 635 ---------------------------SGLAHLHSLHIVHRDLKPGNILITGPDSQGLGR 694

Query: 691 AKLSDMGISKRLLKDMSSLGHHATGCGSSGWQAPE--QLL-HGRQTRAIDLFSLGCVIFF 750
             LSD G+ K+L     S   H+   G+ GW APE  QLL     T A+D+FS GCV ++
Sbjct: 695 VVLSDFGLCKKLPAGRCSFSLHSGIPGTEGWMAPELLQLLPPDSPTSAVDIFSAGCVFYY 754

Query: 751 CLTGGRHPFGDHFERDVNIVRNQMDL-FLVEGIPE---AMDLISQLLNPNPDLRPRASVV 810
            L+GG HPFGD   R  NI+     L  L E + +   A DL+  +L+P P  RP A  V
Sbjct: 755 VLSGGSHPFGDSLYRQANILTGAPCLAHLEEEVHDKVVARDLVGAMLSPLPQPRPSAPQV 814

Query: 811 LQHPLFWSSEVRLSFLRDTSDRVELEDRETHSDLLEALESTAPLALGGKWDEKLDPAFIT 870
           L HP FWS   +L F +D SD +E E  +    L+ ALE+     +   W E +     T
Sbjct: 815 LAHPFFWSRAKQLQFFQDVSDWLEKESEQ--EPLVRALEAGGCAVVRDNWHEHISMPLQT 874

Query: 871 NIGQYRRYKYDSVRDLLRVMRNKLNHYRELPKEIQELIGSVPEGFDNYFASRFPRLLTEV 930
           ++ ++R YK  SVRDLLR +RNK +HYRELP E+++ +G VP+GF  YF +RFPRLL   
Sbjct: 875 DLRKFRSYKGTSVRDLLRAVRNKKHHYRELPVEVRQALGQVPDGFVQYFTNRFPRLLLHT 910

Query: 931 YRVISQYCREEEGFWKYF 938
           +R + + C  E  F  Y+
Sbjct: 935 HRAM-RSCASESLFLPYY 910

BLAST of Cucsa.101350 vs. TrEMBL
Match: A0A0A0KFU7_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_6G486760 PE=4 SV=1)

HSP 1 Score: 1883.6 bits (4878), Expect = 0.0e+00
Identity = 942/942 (100.00%), Postives = 942/942 (100.00%), Query Frame = 1

Query: 1   MRCRFFRRLWLFLLLISRFRSLTAESRTYDNLKFSQLGSYGENSDELAQIRGKSLLPLSL 60
           MRCRFFRRLWLFLLLISRFRSLTAESRTYDNLKFSQLGSYGENSDELAQIRGKSLLPLSL
Sbjct: 1   MRCRFFRRLWLFLLLISRFRSLTAESRTYDNLKFSQLGSYGENSDELAQIRGKSLLPLSL 60

Query: 61  KSKGNTALIAASGGDIYLVDSDSKKIIWSFASGTPIYSAYQSPTNYNKENASGSTRSPFF 120
           KSKGNTALIAASGGDIYLVDSDSKKIIWSFASGTPIYSAYQSPTNYNKENASGSTRSPFF
Sbjct: 61  KSKGNTALIAASGGDIYLVDSDSKKIIWSFASGTPIYSAYQSPTNYNKENASGSTRSPFF 120

Query: 121 FDCGDDWELYIHTEHGRTKLPRTIDEVVRSTPYIFEDGSVMTGSRKTTVYEVNPVTGKLI 180
           FDCGDDWELYIHTEHGRTKLPRTIDEVVRSTPYIFEDGSVMTGSRKTTVYEVNPVTGKLI
Sbjct: 121 FDCGDDWELYIHTEHGRTKLPRTIDEVVRSTPYIFEDGSVMTGSRKTTVYEVNPVTGKLI 180

Query: 181 RNHSSELSPSGLSNDEFSVLNGNSSTNKLENRDLIQPGLMKPIEQRLYITRTDYFLKSSF 240
           RNHSSELSPSGLSNDEFSVLNGNSSTNKLENRDLIQPGLMKPIEQRLYITRTDYFLKSSF
Sbjct: 181 RNHSSELSPSGLSNDEFSVLNGNSSTNKLENRDLIQPGLMKPIEQRLYITRTDYFLKSSF 240

Query: 241 AGSEEVSWSLNVADIGATLVCPDGENPTNSVPLDSQNNGSFEFDFTLPLSCQSEVLVYRE 300
           AGSEEVSWSLNVADIGATLVCPDGENPTNSVPLDSQNNGSFEFDFTLPLSCQSEVLVYRE
Sbjct: 241 AGSEEVSWSLNVADIGATLVCPDGENPTNSVPLDSQNNGSFEFDFTLPLSCQSEVLVYRE 300

Query: 301 RSHVLTESSGHKMLSDSHNTDIMLPASASSLMLPSQPSVKHSNIHPERLMLPGPAANIAS 360
           RSHVLTESSGHKMLSDSHNTDIMLPASASSLMLPSQPSVKHSNIHPERLMLPGPAANIAS
Sbjct: 301 RSHVLTESSGHKMLSDSHNTDIMLPASASSLMLPSQPSVKHSNIHPERLMLPGPAANIAS 360

Query: 361 LLEPNAISQLNDDSQALIPMPLMKINDSSAVLGHNIGTTNVDFIAMVLNGPLGLFIALFI 420
           LLEPNAISQLNDDSQALIPMPLMKINDSSAVLGHNIGTTNVDFIAMVLNGPLGLFIALFI
Sbjct: 361 LLEPNAISQLNDDSQALIPMPLMKINDSSAVLGHNIGTTNVDFIAMVLNGPLGLFIALFI 420

Query: 421 TMFLGLINRGGALVAKLKQFLLKEKQPSAVSSKIVSSKKKKARKLGKNGNFDKKDASASS 480
           TMFLGLINRGGALVAKLKQFLLKEKQPSAVSSKIVSSKKKKARKLGKNGNFDKKDASASS
Sbjct: 421 TMFLGLINRGGALVAKLKQFLLKEKQPSAVSSKIVSSKKKKARKLGKNGNFDKKDASASS 480

Query: 481 ENEDMVRSEGDFNNWFPPNNLIDTSGNGRQIGKLMVTNKEIAKGSNGTIVLEGVYEGRLV 540
           ENEDMVRSEGDFNNWFPPNNLIDTSGNGRQIGKLMVTNKEIAKGSNGTIVLEGVYEGRLV
Sbjct: 481 ENEDMVRSEGDFNNWFPPNNLIDTSGNGRQIGKLMVTNKEIAKGSNGTIVLEGVYEGRLV 540

Query: 541 AVKRLVKTHHDVAFKEVQNLIASDRHQNIVRWYGVEYDQDFVYLSLERCTCSLDDLIQIC 600
           AVKRLVKTHHDVAFKEVQNLIASDRHQNIVRWYGVEYDQDFVYLSLERCTCSLDDLIQIC
Sbjct: 541 AVKRLVKTHHDVAFKEVQNLIASDRHQNIVRWYGVEYDQDFVYLSLERCTCSLDDLIQIC 600

Query: 601 SDPSLNSLLSLDEDAGPMIDYKLRLESLKNVISDLNLWKKNSRPAPLLLGLLRDMVAGLE 660
           SDPSLNSLLSLDEDAGPMIDYKLRLESLKNVISDLNLWKKNSRPAPLLLGLLRDMVAGLE
Sbjct: 601 SDPSLNSLLSLDEDAGPMIDYKLRLESLKNVISDLNLWKKNSRPAPLLLGLLRDMVAGLE 660

Query: 661 HLHELGIIHRDLKPQNVLITKQKSVRAKLSDMGISKRLLKDMSSLGHHATGCGSSGWQAP 720
           HLHELGIIHRDLKPQNVLITKQKSVRAKLSDMGISKRLLKDMSSLGHHATGCGSSGWQAP
Sbjct: 661 HLHELGIIHRDLKPQNVLITKQKSVRAKLSDMGISKRLLKDMSSLGHHATGCGSSGWQAP 720

Query: 721 EQLLHGRQTRAIDLFSLGCVIFFCLTGGRHPFGDHFERDVNIVRNQMDLFLVEGIPEAMD 780
           EQLLHGRQTRAIDLFSLGCVIFFCLTGGRHPFGDHFERDVNIVRNQMDLFLVEGIPEAMD
Sbjct: 721 EQLLHGRQTRAIDLFSLGCVIFFCLTGGRHPFGDHFERDVNIVRNQMDLFLVEGIPEAMD 780

Query: 781 LISQLLNPNPDLRPRASVVLQHPLFWSSEVRLSFLRDTSDRVELEDRETHSDLLEALEST 840
           LISQLLNPNPDLRPRASVVLQHPLFWSSEVRLSFLRDTSDRVELEDRETHSDLLEALEST
Sbjct: 781 LISQLLNPNPDLRPRASVVLQHPLFWSSEVRLSFLRDTSDRVELEDRETHSDLLEALEST 840

Query: 841 APLALGGKWDEKLDPAFITNIGQYRRYKYDSVRDLLRVMRNKLNHYRELPKEIQELIGSV 900
           APLALGGKWDEKLDPAFITNIGQYRRYKYDSVRDLLRVMRNKLNHYRELPKEIQELIGSV
Sbjct: 841 APLALGGKWDEKLDPAFITNIGQYRRYKYDSVRDLLRVMRNKLNHYRELPKEIQELIGSV 900

Query: 901 PEGFDNYFASRFPRLLTEVYRVISQYCREEEGFWKYFKSHVE 943
           PEGFDNYFASRFPRLLTEVYRVISQYCREEEGFWKYFKSHVE
Sbjct: 901 PEGFDNYFASRFPRLLTEVYRVISQYCREEEGFWKYFKSHVE 942

BLAST of Cucsa.101350 vs. TrEMBL
Match: A0A0A0KJP8_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_6G509680 PE=4 SV=1)

HSP 1 Score: 1094.7 bits (2830), Expect = 0.0e+00
Identity = 591/947 (62.41%), Postives = 705/947 (74.45%), Query Frame = 1

Query: 1   MRCRFFRRLWLFLLLISRFRSLTAESRTYDNLKFSQLGSYGENSDELAQIRGKSLLPLSL 60
           M CRFF    L LLLI RF S    S    N + S L  YG NS+ + +I G+SLL L L
Sbjct: 1   MNCRFF---CLLLLLILRFWSFLVSS----NSELSVLNLYGGNSEGVTRIGGRSLLSLPL 60

Query: 61  KSKGNTALIAASGGDIYLVDSDSKKIIWSFASGTPIYSAYQSPTNY--NKENASGSTRSP 120
           K K +TALIAA  G I+LVDS+S KIIWSF+SG PIYS+YQ+  N+  N+ENASG   S 
Sbjct: 61  KGKSSTALIAALDGAIHLVDSNSMKIIWSFSSGPPIYSSYQANINHEPNQENASG-VGSS 120

Query: 121 FFFDCGDDWELYIHTEHGRTKLPRTIDEVVRSTPYIFEDGSVMTGSRKTTVYEVNPVTGK 180
           FFFDCGDDWELYIHTEHG+ KLP TIDEVVR+TPYIFEDG+VMTGSRKT V+EV+ VTG+
Sbjct: 121 FFFDCGDDWELYIHTEHGKMKLPSTIDEVVRNTPYIFEDGAVMTGSRKTAVFEVDLVTGE 180

Query: 181 LIRNHSSELSPSGLSNDEFSVLNGNSSTNKLENRDLIQPGLMKPIEQRLYITRTDYFLKS 240
           LIRNH S+   SGLSN+E        S + ++ +DL+Q   M  +E RLYITRTDY LKS
Sbjct: 181 LIRNHMSKFLSSGLSNEEQV---SYKSKHNMDIKDLMQS--MNSVEPRLYITRTDYSLKS 240

Query: 241 SFAGSEEVSWSLNVADIGATLVCPDGENPTNSVPLDSQNNGSFEFDFTLPLSCQSEVLVY 300
           SF+ SEE SWSLNVA+IGATL+CPD ENP   +P   QNN SF  D+ +PLSCQS+ LV+
Sbjct: 241 SFSNSEEASWSLNVAEIGATLLCPDVENPIEGIPWTLQNNNSFGIDYGVPLSCQSKALVF 300

Query: 301 RERSHVLTESSGHKMLS-DSHNTDIMLPASASSLMLPSQPSV-KHSNIHPERLMLPGPAA 360
           R+RSH L+  SG+K+LS ++H++D M     S   LPSQ  + KH N    + M  G   
Sbjct: 301 RDRSHFLSGPSGYKILSSEAHDSDNM-----SGSFLPSQLKIGKHINAKSGKFMFHG--- 360

Query: 361 NIASLLEPNAISQLNDDSQALIPMPLMKINDSSAVLGHNIGTTNVDFIAMVLNGPLGLFI 420
                        +N+ S A+ P+P MKIN+S+ +    +G         +L    GLF 
Sbjct: 361 ------------LVNNTSYAVDPLPSMKINESNIIQKQKMG---------ILPEAFGLFF 420

Query: 421 ALFITMFLGLINRGGALVAKLKQFLLKEKQPSAVS-SKIVSSKKKKARKLGKNGNFDKKD 480
              +TM +GL+  G  L  K+KQFLLKEK     S S+  SSKK K RKL K+    K++
Sbjct: 421 VFLLTMLVGLMRYGRTLTEKVKQFLLKEKLSLGTSNSRDNSSKKNKPRKLKKSSG--KRE 480

Query: 481 ASASSENEDMV-RSEGDFNNWFPPNNLIDTSGNGRQIGKLMVTNKEIAKGSNGTIVLEGV 540
            S SSE EDM+ + E + N+ F  NNLI    +GR IGKL +TNK+IA GSNGT++LEG+
Sbjct: 481 VSISSEIEDMLLQRENNLNSGFHGNNLI----SGRHIGKLWITNKKIATGSNGTVILEGI 540

Query: 541 YEGRLVAVKRLVKTHHDVAFKEVQNLIASDRHQNIVRWYGVEYDQDFVYLSLERCTCSLD 600
           YEGR VAVKRLVKTHHDVA KEVQNLI SDRH NIVRWYG+E DQDFVYLSLERCTC+L 
Sbjct: 541 YEGRPVAVKRLVKTHHDVASKEVQNLIVSDRHPNIVRWYGMESDQDFVYLSLERCTCNLY 600

Query: 601 DLIQICSDPSLNSLLSLDEDAGPMIDYKLRLESLKNVISDLNLWKKNSRPAPLLLGLLRD 660
           DLIQI SD   N +L LD D+G M  Y + LES+K  + +L LW +N RP+ +LL L+RD
Sbjct: 601 DLIQIYSDLPKNFVLGLDRDSGHMDGYNIHLESIKVALPNLKLWNENGRPSSILLKLMRD 660

Query: 661 MVAGLEHLHELGIIHRDLKPQNVLITKQKSVRAKLSDMGISKRLLKDMSSLGHHATGCGS 720
           +V GL+HLHELGIIHRDLKPQNVLI KQKS+ +KLSDMGISKRL  ++SSLGHHATGCGS
Sbjct: 661 IVVGLKHLHELGIIHRDLKPQNVLILKQKSICSKLSDMGISKRLPANVSSLGHHATGCGS 720

Query: 721 SGWQAPEQLLHGRQTRAIDLFSLGCVIFFCLTGGRHPFGDHFERDVNIVRNQMDLFLVEG 780
           SGWQAPEQLLH RQTRA+DLFSLGCV+FFC+TGGRHPFGD  ERDVNIV N+M+L LV+ 
Sbjct: 721 SGWQAPEQLLHERQTRAVDLFSLGCVLFFCITGGRHPFGDSLERDVNIVNNKMNLLLVDN 780

Query: 781 IPEAMDLISQLLNPNPDLRPRASVVLQHPLFWSSEVRLSFLRDTSDRVELEDRETHSDLL 840
           IPE +DLI +LLNPNP LRP+AS VLQHPLFWS E+RLSFLRDTSDR+ELEDRE  S+LL
Sbjct: 781 IPEVVDLICRLLNPNPGLRPKASKVLQHPLFWSPEMRLSFLRDTSDRIELEDRE--SNLL 840

Query: 841 EALESTAPLALGGKWDEKLDPAFITNIGQYRRYKYDSVRDLLRVMRNKLNHYRELPKEIQ 900
           +ALESTA +ALG KW+EKL+P FI NIG+YRRYKYDSVRDLLRVMRNKLNHYRELPKEIQ
Sbjct: 841 KALESTAQIALGIKWNEKLEPIFIANIGRYRRYKYDSVRDLLRVMRNKLNHYRELPKEIQ 897

Query: 901 ELIGSVPEGFDNYFASRFPRLLTEVYRVISQYCREEEGFWKYFKSHV 942
           EL+GS+PEGFD+YF +RFP+LL EVY+V S +CR EE F KYFKSHV
Sbjct: 901 ELVGSIPEGFDDYFTTRFPKLLIEVYKVTSCFCRHEECFKKYFKSHV 897

BLAST of Cucsa.101350 vs. TrEMBL
Match: F6H4D4_VITVI (Putative uncharacterized protein OS=Vitis vinifera GN=VIT_07s0031g02180 PE=4 SV=1)

HSP 1 Score: 921.0 bits (2379), Expect = 1.2e-264
Identity = 492/894 (55.03%), Postives = 639/894 (71.48%), Query Frame = 1

Query: 58  LSLKSKGNTALIAASGGDIYLVDSDSKKIIWSFASGTPIYSAYQSPTNYNKENASGSTRS 117
           LSL +K +TAL+AA  G I+LV+S+S K++WSF SG  IYS+YQ+P + +     GS   
Sbjct: 52  LSLPNKNDTALVAALNGTIHLVESNSMKVLWSFTSGPSIYSSYQAPLDQDNATDWGSG-- 111

Query: 118 PFFFDCGDDWELYIHTEH-GRTKLPRTIDEVVRSTPYIFEDGSVMTGSRKTTVYEVNPVT 177
            FF DCG+DWELY+H  H G+ KLP T +E + STP++ EDG V+ GS++TTV+ +N  T
Sbjct: 112 -FFVDCGEDWELYMHGRHFGKVKLPMTAEEFISSTPHVSEDGGVILGSKQTTVFLLNAKT 171

Query: 178 GKLIRNHSS-ELSPSGLSNDEFSVLNGNSSTNKLENRDLIQPGLMKPIEQRLYITRTDYF 237
           GKLI ++ S E  P+ LSN E SV++       +++        +  +E RLYITRTDY 
Sbjct: 172 GKLIHSYRSLESPPTPLSNKEESVVHDKDIEEWVDSGSTN----LNIVEPRLYITRTDYS 231

Query: 238 LKSSFAGSEEVSWSLNVADIGATLVCPDGENPTNSVPLD--SQNNGSFEFDFTLPLSCQS 297
           L+S   GS++V W++ VA+IGA  +C   EN  +  PL+   +    +  DF +PL CQS
Sbjct: 232 LQSFAQGSDKVLWNMTVAEIGAAFLCQGTENLFSRPPLNLGCELGPEYNCDFEMPLPCQS 291

Query: 298 EVLVYRERSHVLTES-SGHKMLSDSHNTDIMLPASASSLMLPSQPSV-KHSNIHPERLML 357
           + +VYR R H + E    H  L ++H  D +L           QP++ K  + HP+ +ML
Sbjct: 292 KAVVYRYRGHTMLEPFPRHDRLQEAHQEDRLL----------LQPNIDKTLDFHPQDMML 351

Query: 358 PGPAANIASLLEP-NAIS---QLNDDSQALIPMPLMKINDSSAVLGHNIGTTNVDFIAMV 417
           P    N     EP + IS   Q N+DS+A++P+   KI +S  +   N+     D ++M 
Sbjct: 352 PAVVPNHMLPSEPKDEISLNFQDNNDSEAVLPLSPPKIKNSG-ISDQNVQMPYNDGLSMF 411

Query: 418 LNGPLGLFIALFITMFLGLINRGGALVAKLKQFLLKEKQPSAVSSKIVSSKKKKARKLGK 477
             G +   + +FI + L  +      VA  +  +   KQP+   S  V SKK+K RK  K
Sbjct: 412 SGGSILFSLIVFIVILLVSVIYCCTPVAGEQGEM--NKQPNDSDSNSVPSKKRKIRKSAK 471

Query: 478 NG-NFDKKDASASSENED-MVRSEGDFNNWFPPNNLIDTSGNGRQIGKLMVTNKEIAKGS 537
           N  +  KKD    SEN+D       D + W   N L+D   NGR +GKL V+N  IAKGS
Sbjct: 472 NNISSGKKDEHVLSENKDGSAHIASDNSPWLNLNGLVDGDTNGRIVGKLFVSNIVIAKGS 531

Query: 538 NGTIVLEGVYEGRLVAVKRLVKTHHDVAFKEVQNLIASDRHQNIVRWYGVEYDQDFVYLS 597
           NGTIVLEG++EGR VAVKRLV+ HHDVAFKE+QNLIASDRH NIVRWYGVEYDQDFVYLS
Sbjct: 532 NGTIVLEGIHEGRSVAVKRLVRAHHDVAFKEIQNLIASDRHPNIVRWYGVEYDQDFVYLS 591

Query: 598 LERCTCSLDDLIQICSDPSLNSLLSLDEDAGPMIDYKLRLESLKNVISDLNLWKKNSRPA 657
           LERCTCSL+DL+QI S+ S N   S+D+    M++Y+++L+S+K ++ D+ LWK N  P+
Sbjct: 592 LERCTCSLNDLLQIHSNSSQNPGFSMDQATKAMMEYRIQLDSVKCIVQDIKLWKSNGYPS 651

Query: 658 PLLLGLLRDMVAGLEHLHELGIIHRDLKPQNVLITKQKSVRAKLSDMGISKRLLKDMSSL 717
            +LL L+RD+V+GL HLH+LGIIHRDLKPQNVLI K+KS+ AKLSDMGISKRL+ DMSSL
Sbjct: 652 SVLLSLMRDVVSGLVHLHDLGIIHRDLKPQNVLIIKEKSLCAKLSDMGISKRLVGDMSSL 711

Query: 718 GHHATGCGSSGWQAPEQLLHGRQTRAIDLFSLGCVIFFCLTGGRHPFGDHFERDVNIVRN 777
           GHHATG GSSGWQAPEQLLHGRQTRA+DLFSLGC++F C+TGGRHPFGD  ERDVNIV+N
Sbjct: 712 GHHATGYGSSGWQAPEQLLHGRQTRAVDLFSLGCILFSCITGGRHPFGDPLERDVNIVKN 771

Query: 778 QMDLFLVEGIPEAMDLISQLLNPNPDLRPRASVVLQHPLFWSSEVRLSFLRDTSDRVELE 837
           + DLFLVE IPEA+DL ++LL+P P+LRP+AS VL HPLFWSSE+RLSFLRD SDRVELE
Sbjct: 772 KPDLFLVEFIPEALDLFARLLDPKPELRPKASEVLYHPLFWSSELRLSFLRDASDRVELE 831

Query: 838 DRETHSDLLEALESTAPLALGGKWDEKLDPAFITNIGQYRRYKYDSVRDLLRVMRNKLNH 897
           DRE++S +L+ALE TAP ALGGKW+EK++PAF+ +IG+YRRYK+DSVRDLLRV+RNK NH
Sbjct: 832 DRESNSHVLKALEGTAPTALGGKWNEKMEPAFLADIGRYRRYKFDSVRDLLRVIRNKWNH 891

Query: 898 YRELPKEIQELIGSVPEGFDNYFASRFPRLLTEVYRVISQYCREEEGFWKYFKS 940
           YRELP+EIQE++GSVPEGFD+YF+SRFPRLL EVY+V+S++C+ EE F KYFK+
Sbjct: 892 YRELPREIQEILGSVPEGFDSYFSSRFPRLLIEVYKVVSRHCKGEECFQKYFKA 925

BLAST of Cucsa.101350 vs. TrEMBL
Match: A0A061DF13_THECC (Endoribonuclease/protein kinase IRE1-like, putative OS=Theobroma cacao GN=TCM_000044 PE=4 SV=1)

HSP 1 Score: 905.2 bits (2338), Expect = 6.6e-260
Identity = 507/952 (53.26%), Postives = 652/952 (68.49%), Query Frame = 1

Query: 9   LWLFLLLISRFRSLTAESRTYDNLKFSQLGSYGENSDELAQIRGKSLLPLSLKSKGNTAL 68
           L   LLL S F + TA     + +  ++  +   ++    ++ G+SLL L    +  T L
Sbjct: 5   LCFLLLLASAFSTFTA-----NYMSAAENSAILLSTSPTPRVGGRSLLYLP---EHETEL 64

Query: 69  IAASGGDIYLVDSDSKKIIWSFASGTPIYSAYQSP--TNYNKENASGSTRSPFFFDCGDD 128
            A + G I L    SK++IWSFAS +PIYS+YQ+P  ++   ENAS  T + FF DCGDD
Sbjct: 65  AARADGTIVLRTKKSKRVIWSFASESPIYSSYQAPPPSDNGNENASQPTAA-FFIDCGDD 124

Query: 129 WELYIHTEHG-RTKLPRTIDEVVRSTPYIFEDGSVMTGSRKTTVYEVNPVTGKLIRNHSS 188
           WELY H  H  + KL  T++E V+  P++ EDG++  GS++TTVY V+ ++G+L+  + S
Sbjct: 125 WELYAHATHSNKMKLSVTVEEFVKHMPHVSEDGAITLGSKRTTVYVVDAMSGRLLHVYRS 184

Query: 189 ELSPSGLSND--EFSVLNGNSSTNKLENRDLIQPGLMKPIEQRLYITRTDYFLKSSFAGS 248
             SPS L +D  E S+ + ++      N++L++     P +QR +ITRTDY L+S    S
Sbjct: 185 PDSPSMLESDKKETSLYDNDNG-----NKELLKSAAANPAQQRFHITRTDYTLQSFHPNS 244

Query: 249 EEVSWSLNVADIGATLVCPDGENPTNSVPLDSQNNGSFEF-----DFTLPLSCQSEVLVY 308
           ++++WSL VA+IGA L+C D + P     + S  N S+E      DF LP  CQS+ +V 
Sbjct: 245 DKIAWSLMVAEIGAALLCQDVDVPF----ITSALNSSYELPEIGSDFDLPFPCQSKGVVI 304

Query: 309 RERSHVLTESSGHKMLSDSHNTDIMLPASASSLMLPSQPSVKHSNIHPERLMLPGPAANI 368
           RE+          + ++ SH+ D MLP  AS +          S+ H  R M    A   
Sbjct: 305 REQDT-------SENITTSHHHDPMLPLPASHVPTLQANLGWSSDDHHNRKMHLAAAPEA 364

Query: 369 ASLLEPNA--ISQLND--DSQALIPMPLMKINDSSAVLGHNI----GTTNVDFIAMVLNG 428
              L+P    +S L+D  D++  + +P ++ NDS     H+     G  N     +VL  
Sbjct: 365 KLQLQPKVDNLSNLSDKSDNKTTVLLPPLENNDSRIADVHDSRITDGQRNFSKYLVVLP- 424

Query: 429 PLGLFIALFITMFLGLINRGGALVAKLKQFLLKEKQPSAVSSKIVSSKKKKARKLGK-NG 488
               FI  FI + +G +     LVAK    L   K     +  +  SK+KK+R+LGK NG
Sbjct: 425 ----FILFFIIL-VGFVTYRHILVAKELTAL---KDQPGTNLNVRPSKRKKSRRLGKSNG 484

Query: 489 NFDKKDASASSENEDMVRS-EGDFNNWFPPNNLIDTSGNGRQIGKLMVTNKEIAKGSNGT 548
             +KKD   SSE+ED      GD       N  +D   +GR+IGKL++ + EIAKGSNGT
Sbjct: 485 PVEKKDKHTSSESEDGFSPIYGDNKMLLDLNKFVDGGTDGRRIGKLVLFSTEIAKGSNGT 544

Query: 549 IVLEGVYEGRLVAVKRLVKTHHDVAFKEVQNLIASDRHQNIVRWYGVEYDQDFVYLSLER 608
           IVLEG+YEGR VAVKRLV+ HHDVAFKE+QNLIASDRH NIVRWYGVEYDQDFVYL+LER
Sbjct: 545 IVLEGLYEGRAVAVKRLVQAHHDVAFKEIQNLIASDRHPNIVRWYGVEYDQDFVYLALER 604

Query: 609 CTCSLDDLIQICSDPSLNSLLSLDEDAGPMIDYKLRLESLKNVISDLNLWKKNSRPAPLL 668
           CTCSL DL+Q+ SD S N +LS D+    MI++K+RL+S+K +++D+NLWK N  P+PLL
Sbjct: 605 CTCSLGDLVQMYSDTSQNPVLSEDQATSAMIEHKIRLDSVKGIMTDVNLWKPNGHPSPLL 664

Query: 669 LGLLRDMVAGLEHLHELGIIHRDLKPQNVLITKQKSVRAKLSDMGISKRLLKDMSSLGHH 728
           L L+RD+V+GL HLH+LGIIHRDLKPQNVLI K+K+V AKLSDMGISKRLL+D SSLGH+
Sbjct: 665 LKLMRDVVSGLAHLHDLGIIHRDLKPQNVLIIKEKTVCAKLSDMGISKRLLEDRSSLGHY 724

Query: 729 ATGCGSSGWQAPEQLLHGRQTRAIDLFSLGCVIFFCLTGGRHPFGDHFERDVNIVRNQMD 788
           AT CGSSGWQAPEQLLHGRQTRAIDLFSLGCV+FFC+T GRHPFG+H ERD+N+V NQ++
Sbjct: 725 ATACGSSGWQAPEQLLHGRQTRAIDLFSLGCVLFFCITRGRHPFGNHLERDINVVNNQVN 784

Query: 789 LFLVEGIPEAMDLISQLLNPNPDLRPRASVVLQHPLFWSSEVRLSFLRDTSDRVELEDRE 848
           LFLVE IPEA+DLIS LL P P+LRP A  VL+HPLFWSSE+RLSFLRDTSDRVELEDRE
Sbjct: 785 LFLVEQIPEAVDLISCLLKPEPELRPSALEVLRHPLFWSSEMRLSFLRDTSDRVELEDRE 844

Query: 849 THSDLLEALESTAPLALGGKWDEKLDPAFITNIGQYRRYKYDSVRDLLRVMRNKLNHYRE 908
             SD+L+ALES A +AL GKW EK++PAFI NIG YRRYK+DSVRDLLRVMRNKLNHYRE
Sbjct: 845 ADSDILKALESIATVALCGKWTEKMEPAFIANIGYYRRYKFDSVRDLLRVMRNKLNHYRE 904

Query: 909 LPKEIQELIGSVPEGFDNYFASRFPRLLTEVYRVISQYCREEEGFWKYFKSH 941
           LPKEIQ+L+G VPEGFD YFA+RFPRL  EVY+V+ ++CREEE F KYFKS+
Sbjct: 905 LPKEIQKLVGPVPEGFDGYFATRFPRLFIEVYKVVYRHCREEESFQKYFKSN 922

BLAST of Cucsa.101350 vs. TrEMBL
Match: A0A059AIR2_EUCGR (Uncharacterized protein OS=Eucalyptus grandis GN=EUGRSUZ_J02541 PE=4 SV=1)

HSP 1 Score: 900.6 bits (2326), Expect = 1.6e-258
Identity = 502/958 (52.40%), Postives = 651/958 (67.95%), Query Frame = 1

Query: 1   MRCRFFRRLWLFLLLISRFRSLTAESRTYDNLKFSQLGSYGENSDELAQIRGKSLLPL-S 60
           MR    RRLW  L ++     L+A     ++ + S  G +          RG + LP+ S
Sbjct: 1   MRGGIRRRLWAPLSIVGWLLGLSAVLSRSESSEVSVPGPWD---------RGLTRLPVRS 60

Query: 61  LKS---KGNTALIAASGGDIYLVDSDSKKIIWSFASGTPIYSAYQSPTNYNKENASGS-T 120
           LKS     +TAL+AA  G I+LVDSDS+K+IWS  SG PI+S+Y +P N + EN S S  
Sbjct: 61  LKSIQPNHDTALVAALDGTIHLVDSDSRKVIWSVTSGPPIHSSYHAPINKDNENESSSGP 120

Query: 121 RSPFFFDCGDD-WELYIHTEH-GRTKLPRTIDEVVRSTPYIFEDGSVMTGSRKTTVYEVN 180
            S +F DCGDD WELYIH EH G+ KL   IDE V++TP + EDGSV  GSRK+TV+EV+
Sbjct: 121 SSGYFIDCGDDDWELYIHNEHFGKMKLGMNIDEFVKNTPLVSEDGSVTLGSRKSTVFEVD 180

Query: 181 PVTGKLIRNHSSELSPSGLSND-EFSVLNGNSSTNKLE-NRDLIQPGLMKPIEQRLYITR 240
            +TG++IR +     PS   ND E + L    + +  E N D  QP        RL I R
Sbjct: 181 ALTGRVIRTYGLSDFPSTSPNDIEQTALQDKIANDLAESNSDTAQP--------RLKIMR 240

Query: 241 TDYFLKSSFAGSEEVSWSLNVADIGATLVCPDGENPTNSVPLDSQNNGSFEF--DFTLPL 300
           TDY L+S  A S +V W+++VA+IGA L+C   +N  +   ++S++    E     T+PL
Sbjct: 241 TDYSLQSFAATSNKVLWNVSVAEIGAALLCRGDDNSFSGSSVNSRHQLGLESGNSITMPL 300

Query: 301 SCQSEVLVYRERSHVLTESSGHKMLSDSHNTDIMLPA-SASSLMLPSQ----PSVKHSNI 360
           SCQS  +VYR R   + ++     L D       LP+ S    +LPSQ    PS+     
Sbjct: 301 SCQSRAIVYRFRKRDVYDTFSRPQLHDGRK----LPSPSPKQQLLPSQDEINPSLNFQLE 360

Query: 361 HPE-RLMLPGPAANIASLLEPNAISQLNDDSQALIPMPLMKINDSSAVLGHNIGTTNVDF 420
           +P   LMLP P   I ++      S     +  ++P+P  +I+D+  V   N  T   D 
Sbjct: 361 NPSSELMLP-PLTKIDNMFN----SHDGKAAGVILPLPAPEIHDTVVVRSDNNATVYADG 420

Query: 421 IAMVLNGPLGLFIALFITMFLGLINRGGALVAKLKQFLLKEKQPSAVSSKIVSSKKKKAR 480
            +   +      + + + +F+  +   G +V +  +   ++ + +  SS    SK+KK R
Sbjct: 421 -SRNTSSEWSATLYVILVVFMAAV---GLIVRRFFRVPKEQAKQAGDSSLKTPSKRKKMR 480

Query: 481 KLGK-NGNFDKKDASASSENEDMV-RSEGDFNNWFPPNNLIDTSGNGRQIGKLMVTNKEI 540
           K GK NG+ D+K+   SSENE  +  S  + + W   NN +D    GR+IGKL+V + EI
Sbjct: 481 KYGKSNGSPDRKENHISSENEGRLDNSRSNDDAWMKLNNFLDGGIAGRRIGKLLVLSTEI 540

Query: 541 AKGSNGTIVLEGVYEGRLVAVKRLVKTHHDVAFKEVQNLIASDRHQNIVRWYGVEYDQDF 600
           AKGSNGTIVLEG+YEGR VAVKRLV+ HHDVA KE+QNLIASDRH NIVRWYGVEYDQDF
Sbjct: 541 AKGSNGTIVLEGIYEGRPVAVKRLVQAHHDVALKEIQNLIASDRHANIVRWYGVEYDQDF 600

Query: 601 VYLSLERCTCSLDDLIQICSDPSLNSLLSLDEDAGPMIDYKLRLESLKNVISDLNLWKKN 660
           VYL+LERCTCSLDDLIQ+ SD S NS+     D   M++YK++L+S++ ++ D+NLW+ +
Sbjct: 601 VYLALERCTCSLDDLIQVQSDASNNSVFP--NDPASMVEYKIKLDSVRGMVEDVNLWRAD 660

Query: 661 SRPAPLLLGLLRDMVAGLEHLHELGIIHRDLKPQNVLITKQKSVRAKLSDMGISKRLLKD 720
             P+PLLL L+RD+V+GL HLH+LGIIHRDLKPQNVLITK +S+ AKLSDMGISKRL  D
Sbjct: 661 GHPSPLLLKLMRDVVSGLVHLHDLGIIHRDLKPQNVLITKNRSLCAKLSDMGISKRLHGD 720

Query: 721 MSSLGHHATGCGSSGWQAPEQLLHGRQTRAIDLFSLGCVIFFCLTGGRHPFGDHFERDVN 780
            SSLG+HATGCGSSGWQAPEQLLHGRQTR +D+FSLGCV+FFC+TGGRHPFG+  ERD+N
Sbjct: 721 TSSLGYHATGCGSSGWQAPEQLLHGRQTRTVDVFSLGCVLFFCITGGRHPFGERLERDIN 780

Query: 781 IVRNQMDLFLVEGIPEAMDLISQLLNPNPDLRPRASVVLQHPLFWSSEVRLSFLRDTSDR 840
           IV+N+MDLFLVE IPEA+DL S LLNPNP+LRP+A  VL HPLFWSSE+RLSFLRD SDR
Sbjct: 781 IVKNKMDLFLVEFIPEAVDLFSHLLNPNPELRPKALEVLHHPLFWSSEMRLSFLRDVSDR 840

Query: 841 VELEDRETHSDLLEALESTAPLALGGKWDEKLDPAFITNIGQYRRYKYDSVRDLLRVMRN 900
           VELEDR  +SDLL+A+ESTAP+  GGKWDEK++P F+ +IG++RRY Y  VRDLLRV+RN
Sbjct: 841 VELEDRALNSDLLKAVESTAPMVFGGKWDEKIEPEFMADIGRFRRYNYSCVRDLLRVVRN 900

Query: 901 KLNHYRELPKEIQELIGSVPEGFDNYFASRFPRLLTEVYRVISQYCREEEGFWKYFKS 940
           KL+HYRELP+ +QEL+G VPEG DNYFA RFPRLL E Y+V+S++C+EEE F KYF+S
Sbjct: 901 KLSHYRELPERVQELLGPVPEGCDNYFAKRFPRLLIESYKVVSKFCKEEECFQKYFRS 926

BLAST of Cucsa.101350 vs. TAIR10
Match: AT2G17520.1 (AT2G17520.1 Endoribonuclease/protein kinase IRE1-like)

HSP 1 Score: 660.2 bits (1702), Expect = 1.9e-189
Identity = 330/522 (63.22%), Postives = 412/522 (78.93%), Query Frame = 1

Query: 420 ITMFLGLINRGGALVAKLKQFLLKEKQPSAVSSKIVSSKKKKARKLGKNGNFDKKDASAS 479
           + +   L+  G  +   +K+F     + S VS K   SKKKK RK  K+ N  ++     
Sbjct: 327 VKLLAPLVLLGVVVSVFIKKF---SSRGSDVSLKAGPSKKKKNRKSAKDTN--RQSVPRG 386

Query: 480 SENEDMVRSEGDFNNWFPPNNLIDTSGNGRQIGKLMVTNKEIAKGSNGTIVLEGVYEGRL 539
            +  +++   G     F  NN    + +GR+IGKL +++KEIAKGSNGT+V EG+YEGR 
Sbjct: 387 QDQFELIEG-GQMLLGF--NNFQSGATDGRKIGKLFLSSKEIAKGSNGTVVFEGIYEGRP 446

Query: 540 VAVKRLVKTHHDVAFKEVQNLIASDRHQNIVRWYGVEYDQDFVYLSLERCTCSLDDLIQI 599
           VAVKRLV++HH+VAFKE+QNLIASD+H NI+RWYGVEYDQDFVYLSLERCTCSLDDLI+ 
Sbjct: 447 VAVKRLVRSHHEVAFKEIQNLIASDQHTNIIRWYGVEYDQDFVYLSLERCTCSLDDLIKS 506

Query: 600 CSDPSLNSLLSLDEDAGPMIDYKLRLESLKNVISDLNLWKKNSRPAPLLLGLLRDMVAGL 659
             + S+  +L  ++    +  YK++L+SL+ VI   N WK    P+PL+L L+RD+V G+
Sbjct: 507 YLEFSMTKVLENNDSTEGVAAYKIQLDSLEGVIKGNNFWKVGGHPSPLMLKLMRDIVCGI 566

Query: 660 EHLHELGIIHRDLKPQNVLITKQKSVRAKLSDMGISKRLLKDMSSLGHHATGCGSSGWQA 719
            HLHELGI+HRDLKPQNVLI+K  ++ AKLSDMGISKR+ +DMSSLGH ATG GSSGWQA
Sbjct: 567 VHLHELGIVHRDLKPQNVLISKDMTLSAKLSDMGISKRMSRDMSSLGHLATGSGSSGWQA 626

Query: 720 PEQLLHGRQTRAIDLFSLGCVIFFCLTGGRHPFGDHFERDVNIVRNQMDLFLVEGIPEAM 779
           PEQLL GRQTRA+D+FSLGCVIF+ +TG +HPFGD  ERDVNIV+N++DLFLVE +PEA 
Sbjct: 627 PEQLLQGRQTRAVDMFSLGCVIFYTITGCKHPFGDDLERDVNIVKNKVDLFLVEHVPEAS 686

Query: 780 DLISQLLNPNPDLRPRASVVLQHPLFWSSEVRLSFLRDTSDRVELEDRETHSDLLEALES 839
           DLIS+LLNP+PDLRP A+ VL HP+FW+SE+RLSFLRD SDRVELE+RE  S++L+A+ES
Sbjct: 687 DLISRLLNPDPDLRPSATEVLLHPMFWNSEMRLSFLRDASDRVELENREADSEILKAMES 746

Query: 840 TAPLALGGKWDEKLDPAFITNIGQYRRYKYDSVRDLLRVMRNKLNHYRELPKEIQELIGS 899
           TAP+A+GGKWDEKL+P FITNIG+YRRYKYDS+RDLLRV+RNKLNH+RELP EIQEL+G+
Sbjct: 747 TAPVAIGGKWDEKLEPVFITNIGRYRRYKYDSIRDLLRVIRNKLNHHRELPPEIQELVGT 806

Query: 900 VPEGFDNYFASRFPRLLTEVYRVISQYCREEEGFWKYFKSHV 942
           VPEGFD YFA RFP+LL EVYRVIS +CREEE F KYFK  +
Sbjct: 807 VPEGFDEYFAVRFPKLLIEVYRVISLHCREEEVFRKYFKCDI 840

HSP 2 Score: 78.2 bits (191), Expect = 3.1e-14
Identity = 71/265 (26.79%), Postives = 115/265 (43.40%), Query Frame = 1

Query: 2   RCRFFRRLWLFLLLISRFRSLTAESRTYDNLKFSQLGSYGENSDELAQIRGKSLLPLSLK 61
           RC F R L+  LLL+S +          D        S G  S  + QIR +        
Sbjct: 4   RCPFLRHLFFLLLLLSPWIMSPCGGAADDVTYPIVPSSPGRRS--ILQIRREP------P 63

Query: 62  SKGNTALIAASGGDIYLVDSDSKKIIWSFASGTPIYSAYQSPTNYNKENASGSTRSPFFF 121
           ++ NT L+    G ++L     +   WSF++G+P++S YQ+P N N ENA+  TR     
Sbjct: 64  TEPNTKLVVDRDGKVFLKQQPKETPYWSFSTGSPMHSLYQAPANNNTENATEITRPHIIV 123

Query: 122 DCGDDWELYIHTEHGRTKLPR----TIDEVVRSTPYIFEDGSVMTGSRKTTVYEVNPVTG 181
           +       Y++     T +      T+ E  R  P + +DG V  GS  T+ Y V+  +G
Sbjct: 124 E-------YLNNSKAATTVDGYHNWTVQEFFRQKPLVTDDG-VTLGSETTSAYLVDGRSG 183

Query: 182 KLIRNHSSELSPSGLSNDEFSVLNGNSSTNKLENRDLIQPGLMKP-IEQRLYITRTDYFL 241
           +LI  + S                  +   K+ N  L++P   +  + + L I RTD  L
Sbjct: 184 RLIHVYKS------------------TGDTKITNA-LVKPASTEDFVNEPLLIRRTDSKL 233

Query: 242 KSSFAGSEEVSWSLNVADIGATLVC 262
           +     + ++ W+L V+   A L+C
Sbjct: 244 EHFSKTTGKLVWNLTVSHFRAALLC 233

BLAST of Cucsa.101350 vs. TAIR10
Match: AT5G24360.2 (AT5G24360.2 inositol requiring 1-1)

HSP 1 Score: 592.4 bits (1526), Expect = 4.8e-169
Identity = 310/570 (54.39%), Postives = 406/570 (71.23%), Query Frame = 1

Query: 378 IPMPLMKINDSSAVLGHNIGTTNVDFIAMVLNGPLGLFIALFITMFLGLINRGGALVAKL 437
           +P+P   I+  + ++    G+T     A   +G + L     +TM    ++  G    +L
Sbjct: 335 LPLPETVISQITDIID---GSTKQAGFASKFSGLIVLIFGFCVTM----LSVCGLFFYRL 394

Query: 438 KQFLLKEKQPSAVSSKIVSSKKKKARKLGKNGNFDKKDAS-ASSENEDMVRSEGDFNNWF 497
           +Q + + K+P      I + KKKK++K G      KK+    S  N+D    E +     
Sbjct: 395 RQSI-RIKEPYVSEVPIATPKKKKSKKNGTTKAVHKKENGFISGGNKDPSHEENEKRLLT 454

Query: 498 PPNNLIDTSGNGRQIGKLMVTNKEIAKGSNGTIVLEGVYEGRLVAVKRLVKTHHDVAFKE 557
               L ++S  G ++GKL V+NKEIAKGSNGT+VLEG YEGRLVAVKRLV++HHDVA KE
Sbjct: 455 AFPGLNNSSAEGYRVGKLFVSNKEIAKGSNGTVVLEGSYEGRLVAVKRLVQSHHDVAQKE 514

Query: 558 VQNLIASDRHQNIVRWYGVEYDQDFVYLSLERCTCSLDDLIQICSDPSLNSLLSLDEDAG 617
           + NL+ASD+H NIVRWYGV+ D+ F+Y+SLE C CSL+DLI   S     +LL       
Sbjct: 515 ILNLMASDKHSNIVRWYGVDQDEHFIYISLELCACSLNDLIYASS-----ALLE-----S 574

Query: 618 PMIDYKLRLESLKNVISD---LNLWKKNSRPAPLLLGLLRDMVAGLEHLHELGIIHRDLK 677
           PM    +    +  +  +   + LWK+N  P+P+LL L+RD+VAGL HLH++GI+HRDLK
Sbjct: 575 PMASSSIHSIQINPIFENGKGVELWKENGHPSPVLLKLMRDIVAGLVHLHDIGIVHRDLK 634

Query: 678 PQNVLITKQKSVRAKLSDMGISKRLLKDMSSLGHHATGC--GSSGWQAPEQLLHGRQTRA 737
           PQNVLI K  S+ AKLSDMGISKRL  D S+L  ++TG   GSSGWQAPEQL + RQTRA
Sbjct: 635 PQNVLIVKNSSLCAKLSDMGISKRLPADTSALTRNSTGLGSGSSGWQAPEQLRNERQTRA 694

Query: 738 IDLFSLGCVIFFCLTGGRHPFGDHFERDVNIVRNQMDLFLVEGIPEAMDLISQLLNPNPD 797
           +DLFSLGCV+FFC+TGG+HP+GD++ERDVN++ +Q DLFL+E +PEA+ L++ LLNP+P+
Sbjct: 695 VDLFSLGCVLFFCMTGGKHPYGDNYERDVNVLNDQKDLFLIESLPEAVHLLTGLLNPDPN 754

Query: 798 LRPRASVVLQHPLFWSSEVRLSFLRDTSDRVELEDRETHSDLLEALESTAPLALGGKWDE 857
           LRPRA  V+ HPLFW+S++RLSFLRD SDRVELE+RE  S LL ALESTA + L G+WDE
Sbjct: 755 LRPRAQDVMHHPLFWNSDMRLSFLRDASDRVELENREEGSQLLAALESTAAVTLNGRWDE 814

Query: 858 KLDPAFITNIGQYRRYKYDSVRDLLRVMRNKLNHYRELPKEIQELIGSVPEGFDNYFASR 917
           KLD  F+ NIG+YRRYK+DS+RDLLRV+RNKLNHYRELPKE+QEL+GSVPEGF+ YF+SR
Sbjct: 815 KLDSIFLDNIGRYRRYKFDSIRDLLRVIRNKLNHYRELPKELQELLGSVPEGFERYFSSR 874

Query: 918 FPRLLTEVYRVISQYCREEEGFWKYFKSHV 942
           FP+LL +VY V+  YC  EE F+KY K+ V
Sbjct: 875 FPKLLIQVYTVLFDYCNNEEFFFKYSKTTV 886

HSP 2 Score: 71.6 bits (174), Expect = 2.9e-12
Identity = 57/202 (28.22%), Postives = 97/202 (48.02%), Query Frame = 1

Query: 68  LIAASGGDIYLVDSDSKKIIWSFASGTPIYSAYQSPTNY--NKENASGSTRSPFFFDCGD 127
           L++   G I LVD  S+K+ W+F +  PIYS+YQ+P  +    E  S      F+ DC  
Sbjct: 56  LVSTVDGSISLVDMSSQKLDWTFHTNEPIYSSYQAPHYHYTTDEERSSVLGDDFYMDCDK 115

Query: 128 DWELYIHTEHGRTKLPRTID--EVVRSTPYIFEDGSVMTGSRKTTVYEVNPVTGKLIRNH 187
           DW LY  +     ++   +D  E + + PY   D  ++ G + T+V+ ++  TGKL++ +
Sbjct: 116 DWRLYNSSVRKGKRVNEIVDASEFIGTLPYTSTD-RIVLGKKDTSVFLLDWKTGKLVKRY 175

Query: 188 SSE--LSPSGLSNDEFSVLNGNSSTNKLENRDLIQPGLMK--PIEQRLYITRTDYFLK-- 247
             +   S + + ND+   +         E   L   G  K     + +YI R D+ ++  
Sbjct: 176 RMDELYSNTVVENDKEKAI-----VLSKEAPLLFGSGFKKSEDFPELVYIERKDFKIQCI 235

Query: 248 SSFAGSEEVSWSLNVADIGATL 260
           S F    +V WS++ A + A L
Sbjct: 236 SKFG---DVLWSVSYAKMEAKL 248

BLAST of Cucsa.101350 vs. TAIR10
Match: AT3G11870.1 (AT3G11870.1 Endoribonuclease/protein kinase IRE1-like)

HSP 1 Score: 318.9 bits (816), Expect = 1.0e-86
Identity = 218/532 (40.98%), Postives = 300/532 (56.39%), Query Frame = 1

Query: 443 KEKQPSAVSSKIVSSKKKKA-RKLGKNGNFDKKDASASSENEDM-VRSEGDFNNWFP--- 502
           K   P   +S    +K ++  +K   +G   +++   +SE++ + V S+ + N   P   
Sbjct: 39  KNSAPRVSASGEDGTKNEQVEKKSDPSGGLGEENEKTNSESKVLSVPSDQNINKTLPVML 98

Query: 503 PNNLIDTSGNGRQIGK-----LMVTNKEIAKGSNGTIVLEGVYEGRL-VAVKRLVKTHHD 562
           P+  +         GK     L+V+  E+  G NG  V +GVY  R  VAVK L   H  
Sbjct: 99  PSLELRKYDENETPGKVVNRRLLVSTNEMKYGRNGYEVFQGVYGRRSSVAVKCLDLAHTT 158

Query: 563 VAF--KEVQNLIASDRHQNIVRWYGVEYDQDFVYLSLERCTCSLDDLIQICSDPSLNSLL 622
            AF   E+ N    D H NI+R++G+E DQ F Y+ LE   CSLDDLI++    S+    
Sbjct: 159 EAFIQNEIDNHCLCDDHSNIIRFHGLEQDQSFAYICLEPWKCSLDDLIKL----SVRRTK 218

Query: 623 SLDEDAGPMIDYKLRLESLKNVISDLNLWKKNSRPAPL--LLGLLRDMVAGLEHLHELGI 682
              +   P+ D       L+ V+  +  WK+  +P PL  +L L+RD+V GL HLH+L  
Sbjct: 219 RDTQAVAPVDD-------LEKVMKRIKFWKEKGKPLPLTPMLKLMRDVVCGLAHLHKLKT 278

Query: 683 IHRDLKPQNVLI-TKQKSVRAKLSDMGISKRLLKDMSSLGHHATGCGSSGWQAPEQLLHG 742
           IHR+L PQNVLI  K  ++ AK+SDM +SK L    SS  H AT  GSSGWQAPEQL   
Sbjct: 279 IHRNLNPQNVLIIVKDMTLTAKISDMSLSKHLGGKKSSYKHLATCSGSSGWQAPEQLNKD 338

Query: 743 RQTRA---IDLFSLGCVIFFCLTGGRHPFGDHFERDVNI-VRNQMDLFLVEGIPEAMDLI 802
           ++ +     D+F+ GC++ + + G  HPFG   ERD NI   N+ +L LV  + EA++LI
Sbjct: 339 KKKKEDFPADMFNFGCLLHYAVMG-THPFGSPSERDTNIKTNNKTNLSLVTNL-EAINLI 398

Query: 803 SQLLNPNPDLRPRASVVLQHPLFWSSEVRLSFLRDTSDRVELEDRETHSDLLEALESTAP 862
            QLLN  PDLRP A+ VL HPLFW SE RL FLR+ SDR+EL D     DL + +   AP
Sbjct: 399 EQLLNYKPDLRPSATQVLLHPLFWDSEKRLFFLREASDRIEL-DITMWGDLNKTI---AP 458

Query: 863 LALGGK--WDEKLDPAFITNI------------GQYRR-YKYDSVRDLLRVMRNKLNHYR 922
             LG    W  KL   FIT+I             QY R YKY S+R LLR++RN L+H+R
Sbjct: 459 RVLGESKDWASKLGKTFITHIENLAQAQPGQESRQYNRSYKYWSLRHLLRLIRNILSHHR 518

Query: 923 EL--PKEIQELIGSVPEGFDNYFASRFPRLLTEVYRVISQYCREEEGFWKYF 938
           E+    +I+E++G VPEG D +F +RFP L+ E+Y  IS +C+ EE F KYF
Sbjct: 519 EILDDPKIKEMVGKVPEGLDIFFTARFPNLMMEIYAFISMHCKGEEAFEKYF 553

BLAST of Cucsa.101350 vs. TAIR10
Match: AT3G06030.1 (AT3G06030.1 NPK1-related protein kinase 3)

HSP 1 Score: 90.1 bits (222), Expect = 7.8e-18
Identity = 76/281 (27.05%), Postives = 137/281 (48.75%), Query Frame = 1

Query: 537 GRLVAVKRLV---------KTH-HDVAFKEVQNLIASDRHQNIVRWYGVEYDQDFVYLSL 596
           G L+A+K+++         KT  H    +E   L+ +  H NIVR           YL  
Sbjct: 91  GELLAIKQVLIAPSSASKEKTQGHIRELEEEVQLLKNLSHPNIVR-----------YLGT 150

Query: 597 ERCTCSLDDLIQICSDPSLNSLLSLDEDAGPMIDYKLRLESLKNVISDLNLWKKNSRPAP 656
            R + SL+ L++     S++SLL                             K  S P P
Sbjct: 151 VRESDSLNILMEFVPGGSISSLLE----------------------------KFGSFPEP 210

Query: 657 LLLGLLRDMVAGLEHLHELGIIHRDLKPQNVLITKQKSVRAKLSDMGISKRLLKDMSSLG 716
           +++   + ++ GLE+LH  GI+HRD+K  N+L+  +  +R  L+D G SK+++ +++++ 
Sbjct: 211 VIIMYTKQLLLGLEYLHNNGIMHRDIKGANILVDNKGCIR--LADFGASKKVV-ELATVN 270

Query: 717 HHATGCGSSGWQAPEQLLHGRQTRAIDLFSLGCVIFFCLTGGRHPFGDHFER---DVNIV 776
              +  G+  W APE +L    + + D++S+GC +    T G+ P+ + +++    ++I 
Sbjct: 271 GAKSMKGTPYWMAPEVILQTGHSFSADIWSVGCTVIEMAT-GKPPWSEQYQQFAAVLHIG 328

Query: 777 RNQMDLFLVEGI-PEAMDLISQLLNPNPDLRPRASVVLQHP 804
           R +    + E + PEA D + + L+  P LR  A+ +LQHP
Sbjct: 331 RTKAHPPIPEDLSPEAKDFLMKCLHKEPSLRLSATELLQHP 328

BLAST of Cucsa.101350 vs. TAIR10
Match: AT1G69220.1 (AT1G69220.1 Protein kinase superfamily protein)

HSP 1 Score: 83.2 bits (204), Expect = 9.5e-16
Identity = 99/440 (22.50%), Postives = 183/440 (41.59%), Query Frame = 1

Query: 435 AKLKQFLLKEKQPSAVSSKIVSSKKKKARKLGKNGNFDKKDASASSENEDMVRSEGDFNN 494
           +K K+   K+K+    +     +  +K+   GK    D   +S+    ED  + +   N+
Sbjct: 171 SKDKKGKKKDKEIDMTTMGRAVASMQKSNFGGKTRKLDPSSSSSKLHGEDNRKMQQQ-NS 230

Query: 495 WFPPNNLIDTSGNGRQIGKLMVTNKEIAKGSNGTIV-LEGVYEGRLVAVKRLVKTHHDVA 554
                +L D+        K    N E+ KGS G++     +    +VAVK +  T  +  
Sbjct: 231 KMSTTSLPDSITREDPTTKYEFLN-ELGKGSYGSVYKARDLKTSEIVAVKVISLTEGEEG 290

Query: 555 FKEVQN---LIASDRHQNIVRWYGVEYDQDFVYLSLERCTCSLDDLIQICSDPSLNSLLS 614
           ++E++    ++    H N+VR+ G    +D++++           +++ C   S+  L++
Sbjct: 291 YEEIRGEIEMLQQCNHPNVVRYLGSYQGEDYLWI-----------VMEYCGGGSVADLMN 350

Query: 615 LDEDAGPMIDYKLRLESLKNVISDLNLWKKNSRPAPLLLGLLRDMVAGLEHLHELGIIHR 674
           + E+A  + +Y++                           + R+ + GL +LH +  +HR
Sbjct: 351 VTEEA--LEEYQIAY-------------------------ICREALKGLAYLHSIYKVHR 410

Query: 675 DLKPQNVLITKQKSVRAKLSDMGISKRLLKDMSSLGHHATGCGSSGWQAPEQLLHGRQTR 734
           D+K  N+L+T+Q  V  KL D G++ +L + MS      T  G+  W APE +   R   
Sbjct: 411 DIKGGNILLTEQGEV--KLGDFGVAAQLTRTMSK---RNTFIGTPHWMAPEVIQENRYDG 470

Query: 735 AIDLFSLGCVIFFCLTGGRHPFGDHFERDVNIVRNQMDLFLVEGIPEAM----------- 794
            +D+++LG V    +  G  P           V     LF++   P  M           
Sbjct: 471 KVDVWALG-VSAIEMAEGLPPRSS--------VHPMRVLFMISIEPAPMLEDKEKWSLVF 530

Query: 795 -DLISQLLNPNPDLRPRASVVLQHPLFWSSEVRLSFLRDTSDRVELEDRETHSDLLEALE 854
            D +++ L   P LRP A+ +L+H      +   S +   S ++E   +   +  L+A  
Sbjct: 531 HDFVAKCLTKEPRLRPTAAEMLKHKFVERCKTGASAM---SPKIEKSRQIRATMALQAQS 553

BLAST of Cucsa.101350 vs. NCBI nr
Match: gi|449448588|ref|XP_004142048.1| (PREDICTED: serine/threonine-protein kinase/endoribonuclease IRE1a [Cucumis sativus])

HSP 1 Score: 1883.6 bits (4878), Expect = 0.0e+00
Identity = 942/942 (100.00%), Postives = 942/942 (100.00%), Query Frame = 1

Query: 1   MRCRFFRRLWLFLLLISRFRSLTAESRTYDNLKFSQLGSYGENSDELAQIRGKSLLPLSL 60
           MRCRFFRRLWLFLLLISRFRSLTAESRTYDNLKFSQLGSYGENSDELAQIRGKSLLPLSL
Sbjct: 1   MRCRFFRRLWLFLLLISRFRSLTAESRTYDNLKFSQLGSYGENSDELAQIRGKSLLPLSL 60

Query: 61  KSKGNTALIAASGGDIYLVDSDSKKIIWSFASGTPIYSAYQSPTNYNKENASGSTRSPFF 120
           KSKGNTALIAASGGDIYLVDSDSKKIIWSFASGTPIYSAYQSPTNYNKENASGSTRSPFF
Sbjct: 61  KSKGNTALIAASGGDIYLVDSDSKKIIWSFASGTPIYSAYQSPTNYNKENASGSTRSPFF 120

Query: 121 FDCGDDWELYIHTEHGRTKLPRTIDEVVRSTPYIFEDGSVMTGSRKTTVYEVNPVTGKLI 180
           FDCGDDWELYIHTEHGRTKLPRTIDEVVRSTPYIFEDGSVMTGSRKTTVYEVNPVTGKLI
Sbjct: 121 FDCGDDWELYIHTEHGRTKLPRTIDEVVRSTPYIFEDGSVMTGSRKTTVYEVNPVTGKLI 180

Query: 181 RNHSSELSPSGLSNDEFSVLNGNSSTNKLENRDLIQPGLMKPIEQRLYITRTDYFLKSSF 240
           RNHSSELSPSGLSNDEFSVLNGNSSTNKLENRDLIQPGLMKPIEQRLYITRTDYFLKSSF
Sbjct: 181 RNHSSELSPSGLSNDEFSVLNGNSSTNKLENRDLIQPGLMKPIEQRLYITRTDYFLKSSF 240

Query: 241 AGSEEVSWSLNVADIGATLVCPDGENPTNSVPLDSQNNGSFEFDFTLPLSCQSEVLVYRE 300
           AGSEEVSWSLNVADIGATLVCPDGENPTNSVPLDSQNNGSFEFDFTLPLSCQSEVLVYRE
Sbjct: 241 AGSEEVSWSLNVADIGATLVCPDGENPTNSVPLDSQNNGSFEFDFTLPLSCQSEVLVYRE 300

Query: 301 RSHVLTESSGHKMLSDSHNTDIMLPASASSLMLPSQPSVKHSNIHPERLMLPGPAANIAS 360
           RSHVLTESSGHKMLSDSHNTDIMLPASASSLMLPSQPSVKHSNIHPERLMLPGPAANIAS
Sbjct: 301 RSHVLTESSGHKMLSDSHNTDIMLPASASSLMLPSQPSVKHSNIHPERLMLPGPAANIAS 360

Query: 361 LLEPNAISQLNDDSQALIPMPLMKINDSSAVLGHNIGTTNVDFIAMVLNGPLGLFIALFI 420
           LLEPNAISQLNDDSQALIPMPLMKINDSSAVLGHNIGTTNVDFIAMVLNGPLGLFIALFI
Sbjct: 361 LLEPNAISQLNDDSQALIPMPLMKINDSSAVLGHNIGTTNVDFIAMVLNGPLGLFIALFI 420

Query: 421 TMFLGLINRGGALVAKLKQFLLKEKQPSAVSSKIVSSKKKKARKLGKNGNFDKKDASASS 480
           TMFLGLINRGGALVAKLKQFLLKEKQPSAVSSKIVSSKKKKARKLGKNGNFDKKDASASS
Sbjct: 421 TMFLGLINRGGALVAKLKQFLLKEKQPSAVSSKIVSSKKKKARKLGKNGNFDKKDASASS 480

Query: 481 ENEDMVRSEGDFNNWFPPNNLIDTSGNGRQIGKLMVTNKEIAKGSNGTIVLEGVYEGRLV 540
           ENEDMVRSEGDFNNWFPPNNLIDTSGNGRQIGKLMVTNKEIAKGSNGTIVLEGVYEGRLV
Sbjct: 481 ENEDMVRSEGDFNNWFPPNNLIDTSGNGRQIGKLMVTNKEIAKGSNGTIVLEGVYEGRLV 540

Query: 541 AVKRLVKTHHDVAFKEVQNLIASDRHQNIVRWYGVEYDQDFVYLSLERCTCSLDDLIQIC 600
           AVKRLVKTHHDVAFKEVQNLIASDRHQNIVRWYGVEYDQDFVYLSLERCTCSLDDLIQIC
Sbjct: 541 AVKRLVKTHHDVAFKEVQNLIASDRHQNIVRWYGVEYDQDFVYLSLERCTCSLDDLIQIC 600

Query: 601 SDPSLNSLLSLDEDAGPMIDYKLRLESLKNVISDLNLWKKNSRPAPLLLGLLRDMVAGLE 660
           SDPSLNSLLSLDEDAGPMIDYKLRLESLKNVISDLNLWKKNSRPAPLLLGLLRDMVAGLE
Sbjct: 601 SDPSLNSLLSLDEDAGPMIDYKLRLESLKNVISDLNLWKKNSRPAPLLLGLLRDMVAGLE 660

Query: 661 HLHELGIIHRDLKPQNVLITKQKSVRAKLSDMGISKRLLKDMSSLGHHATGCGSSGWQAP 720
           HLHELGIIHRDLKPQNVLITKQKSVRAKLSDMGISKRLLKDMSSLGHHATGCGSSGWQAP
Sbjct: 661 HLHELGIIHRDLKPQNVLITKQKSVRAKLSDMGISKRLLKDMSSLGHHATGCGSSGWQAP 720

Query: 721 EQLLHGRQTRAIDLFSLGCVIFFCLTGGRHPFGDHFERDVNIVRNQMDLFLVEGIPEAMD 780
           EQLLHGRQTRAIDLFSLGCVIFFCLTGGRHPFGDHFERDVNIVRNQMDLFLVEGIPEAMD
Sbjct: 721 EQLLHGRQTRAIDLFSLGCVIFFCLTGGRHPFGDHFERDVNIVRNQMDLFLVEGIPEAMD 780

Query: 781 LISQLLNPNPDLRPRASVVLQHPLFWSSEVRLSFLRDTSDRVELEDRETHSDLLEALEST 840
           LISQLLNPNPDLRPRASVVLQHPLFWSSEVRLSFLRDTSDRVELEDRETHSDLLEALEST
Sbjct: 781 LISQLLNPNPDLRPRASVVLQHPLFWSSEVRLSFLRDTSDRVELEDRETHSDLLEALEST 840

Query: 841 APLALGGKWDEKLDPAFITNIGQYRRYKYDSVRDLLRVMRNKLNHYRELPKEIQELIGSV 900
           APLALGGKWDEKLDPAFITNIGQYRRYKYDSVRDLLRVMRNKLNHYRELPKEIQELIGSV
Sbjct: 841 APLALGGKWDEKLDPAFITNIGQYRRYKYDSVRDLLRVMRNKLNHYRELPKEIQELIGSV 900

Query: 901 PEGFDNYFASRFPRLLTEVYRVISQYCREEEGFWKYFKSHVE 943
           PEGFDNYFASRFPRLLTEVYRVISQYCREEEGFWKYFKSHVE
Sbjct: 901 PEGFDNYFASRFPRLLTEVYRVISQYCREEEGFWKYFKSHVE 942

BLAST of Cucsa.101350 vs. NCBI nr
Match: gi|659080799|ref|XP_008440986.1| (PREDICTED: serine/threonine-protein kinase/endoribonuclease IRE1a-like [Cucumis melo])

HSP 1 Score: 1826.2 bits (4729), Expect = 0.0e+00
Identity = 910/942 (96.60%), Postives = 924/942 (98.09%), Query Frame = 1

Query: 1   MRCRFFRRLWLFLLLISRFRSLTAESRTYDNLKFSQLGSYGENSDELAQIRGKSLLPLSL 60
           MRCRFFRRLWLFLLLISRFRSLTAESRTYDNLKFSQLGSYGENSDELA+I GKSLLPLSL
Sbjct: 1   MRCRFFRRLWLFLLLISRFRSLTAESRTYDNLKFSQLGSYGENSDELAEIGGKSLLPLSL 60

Query: 61  KSKGNTALIAASGGDIYLVDSDSKKIIWSFASGTPIYSAYQSPTNYNKENASGSTRSPFF 120
           KSKGNTALIAASGGDIYLVDSDSKKIIWSF+SGTPIYS+YQSPTNYNKENASGSTRSPFF
Sbjct: 61  KSKGNTALIAASGGDIYLVDSDSKKIIWSFSSGTPIYSSYQSPTNYNKENASGSTRSPFF 120

Query: 121 FDCGDDWELYIHTEHGRTKLPRTIDEVVRSTPYIFEDGSVMTGSRKTTVYEVNPVTGKLI 180
           FDCGDDWELYIHTEHGRTKLPRTIDEVVRSTPYIFEDGSVMTGSRKTTVYEVNPVTGKLI
Sbjct: 121 FDCGDDWELYIHTEHGRTKLPRTIDEVVRSTPYIFEDGSVMTGSRKTTVYEVNPVTGKLI 180

Query: 181 RNHSSELSPSGLSNDEFSVLNGNSSTNKLENRDLIQPGLMKPIEQRLYITRTDYFLKSSF 240
           RNHSSELSPSGLSN++FSVLNGNSS N L NRDLIQPGLMKPIEQRLYITRTDYFLKSSF
Sbjct: 181 RNHSSELSPSGLSNEDFSVLNGNSSKNNLANRDLIQPGLMKPIEQRLYITRTDYFLKSSF 240

Query: 241 AGSEEVSWSLNVADIGATLVCPDGENPTNSVPLDSQNNGSFEFDFTLPLSCQSEVLVYRE 300
           AGSEEVSWSLNVADIGATLVCPDGENPTNSVPLDSQNNGSFEFDFT PLSCQSEVLVYRE
Sbjct: 241 AGSEEVSWSLNVADIGATLVCPDGENPTNSVPLDSQNNGSFEFDFTPPLSCQSEVLVYRE 300

Query: 301 RSHVLTESSGHKMLSDSHNTDIMLPASASSLMLPSQPSVKHSNIHPERLMLPGPAANIAS 360
           RSHVLTES GHKMLSDSHNTDI+LP SASSLMLPSQPSVKHSNIHPERLMLPGPAANIAS
Sbjct: 301 RSHVLTESFGHKMLSDSHNTDIVLPTSASSLMLPSQPSVKHSNIHPERLMLPGPAANIAS 360

Query: 361 LLEPNAISQLNDDSQALIPMPLMKINDSSAVLGHNIGTTNVDFIAMVLNGPLGLFIALFI 420
           LLEPN ISQLNDDSQAL+P+PLMKINDSSAV GHNIGT NVDFIAMVLNGPLGLFIALFI
Sbjct: 361 LLEPNTISQLNDDSQALVPLPLMKINDSSAVQGHNIGTANVDFIAMVLNGPLGLFIALFI 420

Query: 421 TMFLGLINRGGALVAKLKQFLLKEKQPSAVSSKIVSSKKKKARKLGKNGNFDKKDASASS 480
           TMFLGLINRGGALVAKLKQFL KEKQPS VSSKIVSSKKKKARKLGKNGNFDKKDAS SS
Sbjct: 421 TMFLGLINRGGALVAKLKQFLFKEKQPSTVSSKIVSSKKKKARKLGKNGNFDKKDASVSS 480

Query: 481 ENEDMVRSEGDFNNWFPPNNLIDTSGNGRQIGKLMVTNKEIAKGSNGTIVLEGVYEGRLV 540
           ENEDMVRSEGDFNNWFPPNNLIDT+GNGRQIGKLMVTN EIAKGSNGTIVLEGVYEGRLV
Sbjct: 481 ENEDMVRSEGDFNNWFPPNNLIDTTGNGRQIGKLMVTNTEIAKGSNGTIVLEGVYEGRLV 540

Query: 541 AVKRLVKTHHDVAFKEVQNLIASDRHQNIVRWYGVEYDQDFVYLSLERCTCSLDDLIQIC 600
           AVKRLVKTHHDVAFKEVQNLIASDRHQNIVRWYGVEYDQDFVYLSLERCTCSLDDLIQIC
Sbjct: 541 AVKRLVKTHHDVAFKEVQNLIASDRHQNIVRWYGVEYDQDFVYLSLERCTCSLDDLIQIC 600

Query: 601 SDPSLNSLLSLDEDAGPMIDYKLRLESLKNVISDLNLWKKNSRPAPLLLGLLRDMVAGLE 660
           SDPSLN+LLSLDEDAGPMIDYKLRLESLKNVISDLNLWKKNSRP+PLLLGL+RDMVAGLE
Sbjct: 601 SDPSLNTLLSLDEDAGPMIDYKLRLESLKNVISDLNLWKKNSRPSPLLLGLMRDMVAGLE 660

Query: 661 HLHELGIIHRDLKPQNVLITKQKSVRAKLSDMGISKRLLKDMSSLGHHATGCGSSGWQAP 720
           HLHELGIIHRDLKPQNVLITKQKSVRAKLSDMGISKRLLKDMSSLGHHATGCGSSGWQAP
Sbjct: 661 HLHELGIIHRDLKPQNVLITKQKSVRAKLSDMGISKRLLKDMSSLGHHATGCGSSGWQAP 720

Query: 721 EQLLHGRQTRAIDLFSLGCVIFFCLTGGRHPFGDHFERDVNIVRNQMDLFLVEGIPEAMD 780
           EQLLHGRQTRAIDLFSLGCVIFFCLTGGRHPFGD FERDVNIVRNQMDLFLVEGIPEA+D
Sbjct: 721 EQLLHGRQTRAIDLFSLGCVIFFCLTGGRHPFGDRFERDVNIVRNQMDLFLVEGIPEAVD 780

Query: 781 LISQLLNPNPDLRPRASVVLQHPLFWSSEVRLSFLRDTSDRVELEDRETHSDLLEALEST 840
           LISQLLNPNPD+RPRAS VLQHPLFWSSEVRLSFLRDTSDRVELEDRE HSDLLEALEST
Sbjct: 781 LISQLLNPNPDVRPRASEVLQHPLFWSSEVRLSFLRDTSDRVELEDRENHSDLLEALEST 840

Query: 841 APLALGGKWDEKLDPAFITNIGQYRRYKYDSVRDLLRVMRNKLNHYRELPKEIQELIGSV 900
           APLALGGKWDEKLDPAFITNIGQYRRYKYDSVRDLLRVMRNKLNHYRELPKEIQELIGSV
Sbjct: 841 APLALGGKWDEKLDPAFITNIGQYRRYKYDSVRDLLRVMRNKLNHYRELPKEIQELIGSV 900

Query: 901 PEGFDNYFASRFPRLLTEVYRVISQYCREEEGFWKYFKSHVE 943
           PEGFDNYFASRFPRLLTEVYRVISQYCREEEGF KYFKSHVE
Sbjct: 901 PEGFDNYFASRFPRLLTEVYRVISQYCREEEGFRKYFKSHVE 942

BLAST of Cucsa.101350 vs. NCBI nr
Match: gi|659080666|ref|XP_008440915.1| (PREDICTED: serine/threonine-protein kinase/endoribonuclease IRE1a-like isoform X1 [Cucumis melo])

HSP 1 Score: 1159.8 bits (2999), Expect = 0.0e+00
Identity = 603/948 (63.61%), Postives = 727/948 (76.69%), Query Frame = 1

Query: 1   MRCRFFRRLWLFLLLISRFRSLTAESRTYDNLKFSQLGSYGENSDELAQIRGKSLLPLSL 60
           M+CRFF    L LLLI RF S    S++++N +FS L  YG NS+ + +I G+SLL L L
Sbjct: 1   MKCRFF---CLLLLLILRFWSFLVSSKSFENSEFSVLNPYGVNSEGVTRIGGRSLLSLPL 60

Query: 61  KSKGNTALIAASGGDIYLVDSDSKKIIWSFASGTPIYSAYQSPTNY--NKENASGSTRSP 120
           K K +TALIAA  G I+LVDS+S KIIWSF+SG PIYS+YQ+  N+  N+ENASG   S 
Sbjct: 61  KGKSSTALIAALDGTIHLVDSNSMKIIWSFSSGPPIYSSYQANINHEHNQENASG-VGSS 120

Query: 121 FFFDCGDDWELYIHTEHGRTKLPRTIDEVVRSTPYIFEDGSVMTGSRKTTVYEVNPVTGK 180
           FFFDCGDDWELYIHTEHG+ KLP TIDEVVR+TPYIFEDG+VMTGSRKTTV+EV+ VTG+
Sbjct: 121 FFFDCGDDWELYIHTEHGKMKLPSTIDEVVRNTPYIFEDGAVMTGSRKTTVFEVDLVTGE 180

Query: 181 LIRNHSSELSPSGLSNDEFSVLNGNSSTNKLENRDLIQPGLMKPIEQRLYITRTDYFLKS 240
           LIRNH S+   S LSN+E        S   ++ +DL+Q  +M P+E RLYITRTDY LKS
Sbjct: 181 LIRNHMSKFLSSELSNEE---PGSYKSKQNMDIKDLMQSSMMNPVEPRLYITRTDYSLKS 240

Query: 241 SFAGSEEVSWSLNVADIGATLVCPDGENPTNSVPLDSQNNGSFEFDFTLPLSCQSEVLVY 300
           SF+ SEE SWSLNVA+IGATL+CPD ENP   +P   QNN SF  DF +PLSCQS+ LV+
Sbjct: 241 SFSNSEEASWSLNVAEIGATLLCPDVENPIEGIPWTLQNNNSFGIDFAMPLSCQSKALVF 300

Query: 301 RERSHVLTESSGHKML-SDSHNTDIMLPASASSLMLPSQPSV-KHSNIHPERLMLPGPAA 360
           R+RSH L+  SG+K L S++HN++ +   SAS   LPSQ +V KH N   E+ ML GP  
Sbjct: 301 RDRSHFLSGPSGYKRLPSEAHNSNNISLVSASGSFLPSQLTVGKHINTGSEKFMLTGP-- 360

Query: 361 NIASLLEPNAISQLNDDSQALIPMPLMKINDSSAVLGHNIGTTNVDFIAMVLNGPLGLFI 420
                        +N+ S  ++P+P MKIN+S+ +    +GT         L G  GLF 
Sbjct: 361 -------------VNNTSYRVVPLPSMKINESNIIQEQKMGT---------LPGAFGLFF 420

Query: 421 ALFITMFLGLINRGGALVAKLKQFLLKEKQPSAVS-SKIVSSKKKKARKLGKNGNFDKKD 480
              +TM +GL+  G  L  K+KQ  LKE+     S S+++SSKK K RK  K+G+  K++
Sbjct: 421 VFLLTMLVGLMKYGRTLAVKVKQSFLKEQSSLGTSNSRVISSKKNKPRKSKKSGSSGKRE 480

Query: 481 ASASSENEDMV-RSEGDFNNWFPPNNLIDTSGNGRQIGKLMVTNKEIAKGSNGTIVLEGV 540
            S SSE EDM+ + E + NN F  NNL + +G+GR++GKL VTNKEIA GSNGTI+LEG+
Sbjct: 481 VSISSEIEDMLLQREDNLNNGFHDNNLTNIAGSGRRVGKLWVTNKEIATGSNGTIILEGI 540

Query: 541 YEGRLVAVKRLVKTHHDVAFKEVQNLIASDRHQNIVRWYGVEYDQDFVYLSLERCTCSLD 600
           YEGR VAVKRLVKTHHD+  KEVQNLI SDRH NIVRWYG++ DQDFVYLSLERCTCSL 
Sbjct: 541 YEGRPVAVKRLVKTHHDIGSKEVQNLIVSDRHPNIVRWYGMDSDQDFVYLSLERCTCSLY 600

Query: 601 DLIQICSDPSLNSLLSLDEDAGPMIDYKLRLESLKNVISDLNLWKKNSRPAPLLLGLLRD 660
           DLI+I SD  +NS+L LD+D G M +Y + LES+K  + +L LW +N RP+ +LL L+RD
Sbjct: 601 DLIKIYSDLPMNSMLGLDQDTGRMDEYNIHLESIKVAMPNLKLWNENGRPSSVLLKLMRD 660

Query: 661 MVAGLEHLHELGIIHRDLKPQNVLITKQKSVRAKLSDMGISKRLLKDMSSLGHHATGCGS 720
           +VAGLEHLHELGIIHRDLKPQNVLI KQ+S+ +KLSDMGISKRL  ++SSLGH ATGCGS
Sbjct: 661 IVAGLEHLHELGIIHRDLKPQNVLIVKQQSICSKLSDMGISKRLPANVSSLGHRATGCGS 720

Query: 721 SGWQAPEQLLHGRQTRAIDLFSLGCVIFFCLTGGRHPFGDHFERDVNIVRNQMDLFLVEG 780
           SGWQAPEQLLHGRQTRAIDLFSLGCV+FFC+TGGRHPFGD  ERDVNIV N+M+L LV+ 
Sbjct: 721 SGWQAPEQLLHGRQTRAIDLFSLGCVLFFCVTGGRHPFGDSLERDVNIVNNKMNLLLVDN 780

Query: 781 IPEAMDLISQLLNPNPDLRPRASVVLQHPLFWSSEVRLSFLRDTSDRVELEDRETHSDLL 840
           IPE +DLISQLLNPNP LRP+AS VLQHPLFWS E+RLSFLRDTSDR+ELEDRET  DLL
Sbjct: 781 IPEVVDLISQLLNPNPGLRPKASEVLQHPLFWSPEMRLSFLRDTSDRIELEDRET--DLL 840

Query: 841 EALESTAPLALGGKWDEKLDPAFITNIGQYRRYKYDSVRDLLRVMRNKLNHYRELPKEIQ 900
           +ALESTA +ALG KW+EKL+P FI NIG+YRRYKYDSVRDLLRVMRNKLNHYRELPKEIQ
Sbjct: 841 KALESTAQIALGLKWNEKLEPIFIANIGRYRRYKYDSVRDLLRVMRNKLNHYRELPKEIQ 900

Query: 901 ELIGSVPEGFDNYFASRFPRLLTEVYRVISQYCREEEGFWKYFKSHVE 943
           EL+GS+P GFD+YF  RFP+LL EVY+VIS +CR+EE F KYFKSHV+
Sbjct: 901 ELVGSIPVGFDDYFTRRFPKLLIEVYKVISCFCRQEECFQKYFKSHVD 915

BLAST of Cucsa.101350 vs. NCBI nr
Match: gi|449434428|ref|XP_004134998.1| (PREDICTED: serine/threonine-protein kinase/endoribonuclease IRE1a-like isoform X1 [Cucumis sativus])

HSP 1 Score: 1094.7 bits (2830), Expect = 0.0e+00
Identity = 591/947 (62.41%), Postives = 705/947 (74.45%), Query Frame = 1

Query: 1   MRCRFFRRLWLFLLLISRFRSLTAESRTYDNLKFSQLGSYGENSDELAQIRGKSLLPLSL 60
           M CRFF    L LLLI RF S    S    N + S L  YG NS+ + +I G+SLL L L
Sbjct: 1   MNCRFF---CLLLLLILRFWSFLVSS----NSELSVLNLYGGNSEGVTRIGGRSLLSLPL 60

Query: 61  KSKGNTALIAASGGDIYLVDSDSKKIIWSFASGTPIYSAYQSPTNY--NKENASGSTRSP 120
           K K +TALIAA  G I+LVDS+S KIIWSF+SG PIYS+YQ+  N+  N+ENASG   S 
Sbjct: 61  KGKSSTALIAALDGAIHLVDSNSMKIIWSFSSGPPIYSSYQANINHEPNQENASG-VGSS 120

Query: 121 FFFDCGDDWELYIHTEHGRTKLPRTIDEVVRSTPYIFEDGSVMTGSRKTTVYEVNPVTGK 180
           FFFDCGDDWELYIHTEHG+ KLP TIDEVVR+TPYIFEDG+VMTGSRKT V+EV+ VTG+
Sbjct: 121 FFFDCGDDWELYIHTEHGKMKLPSTIDEVVRNTPYIFEDGAVMTGSRKTAVFEVDLVTGE 180

Query: 181 LIRNHSSELSPSGLSNDEFSVLNGNSSTNKLENRDLIQPGLMKPIEQRLYITRTDYFLKS 240
           LIRNH S+   SGLSN+E        S + ++ +DL+Q   M  +E RLYITRTDY LKS
Sbjct: 181 LIRNHMSKFLSSGLSNEEQV---SYKSKHNMDIKDLMQS--MNSVEPRLYITRTDYSLKS 240

Query: 241 SFAGSEEVSWSLNVADIGATLVCPDGENPTNSVPLDSQNNGSFEFDFTLPLSCQSEVLVY 300
           SF+ SEE SWSLNVA+IGATL+CPD ENP   +P   QNN SF  D+ +PLSCQS+ LV+
Sbjct: 241 SFSNSEEASWSLNVAEIGATLLCPDVENPIEGIPWTLQNNNSFGIDYGVPLSCQSKALVF 300

Query: 301 RERSHVLTESSGHKMLS-DSHNTDIMLPASASSLMLPSQPSV-KHSNIHPERLMLPGPAA 360
           R+RSH L+  SG+K+LS ++H++D M     S   LPSQ  + KH N    + M  G   
Sbjct: 301 RDRSHFLSGPSGYKILSSEAHDSDNM-----SGSFLPSQLKIGKHINAKSGKFMFHG--- 360

Query: 361 NIASLLEPNAISQLNDDSQALIPMPLMKINDSSAVLGHNIGTTNVDFIAMVLNGPLGLFI 420
                        +N+ S A+ P+P MKIN+S+ +    +G         +L    GLF 
Sbjct: 361 ------------LVNNTSYAVDPLPSMKINESNIIQKQKMG---------ILPEAFGLFF 420

Query: 421 ALFITMFLGLINRGGALVAKLKQFLLKEKQPSAVS-SKIVSSKKKKARKLGKNGNFDKKD 480
              +TM +GL+  G  L  K+KQFLLKEK     S S+  SSKK K RKL K+    K++
Sbjct: 421 VFLLTMLVGLMRYGRTLTEKVKQFLLKEKLSLGTSNSRDNSSKKNKPRKLKKSSG--KRE 480

Query: 481 ASASSENEDMV-RSEGDFNNWFPPNNLIDTSGNGRQIGKLMVTNKEIAKGSNGTIVLEGV 540
            S SSE EDM+ + E + N+ F  NNLI    +GR IGKL +TNK+IA GSNGT++LEG+
Sbjct: 481 VSISSEIEDMLLQRENNLNSGFHGNNLI----SGRHIGKLWITNKKIATGSNGTVILEGI 540

Query: 541 YEGRLVAVKRLVKTHHDVAFKEVQNLIASDRHQNIVRWYGVEYDQDFVYLSLERCTCSLD 600
           YEGR VAVKRLVKTHHDVA KEVQNLI SDRH NIVRWYG+E DQDFVYLSLERCTC+L 
Sbjct: 541 YEGRPVAVKRLVKTHHDVASKEVQNLIVSDRHPNIVRWYGMESDQDFVYLSLERCTCNLY 600

Query: 601 DLIQICSDPSLNSLLSLDEDAGPMIDYKLRLESLKNVISDLNLWKKNSRPAPLLLGLLRD 660
           DLIQI SD   N +L LD D+G M  Y + LES+K  + +L LW +N RP+ +LL L+RD
Sbjct: 601 DLIQIYSDLPKNFVLGLDRDSGHMDGYNIHLESIKVALPNLKLWNENGRPSSILLKLMRD 660

Query: 661 MVAGLEHLHELGIIHRDLKPQNVLITKQKSVRAKLSDMGISKRLLKDMSSLGHHATGCGS 720
           +V GL+HLHELGIIHRDLKPQNVLI KQKS+ +KLSDMGISKRL  ++SSLGHHATGCGS
Sbjct: 661 IVVGLKHLHELGIIHRDLKPQNVLILKQKSICSKLSDMGISKRLPANVSSLGHHATGCGS 720

Query: 721 SGWQAPEQLLHGRQTRAIDLFSLGCVIFFCLTGGRHPFGDHFERDVNIVRNQMDLFLVEG 780
           SGWQAPEQLLH RQTRA+DLFSLGCV+FFC+TGGRHPFGD  ERDVNIV N+M+L LV+ 
Sbjct: 721 SGWQAPEQLLHERQTRAVDLFSLGCVLFFCITGGRHPFGDSLERDVNIVNNKMNLLLVDN 780

Query: 781 IPEAMDLISQLLNPNPDLRPRASVVLQHPLFWSSEVRLSFLRDTSDRVELEDRETHSDLL 840
           IPE +DLI +LLNPNP LRP+AS VLQHPLFWS E+RLSFLRDTSDR+ELEDRE  S+LL
Sbjct: 781 IPEVVDLICRLLNPNPGLRPKASKVLQHPLFWSPEMRLSFLRDTSDRIELEDRE--SNLL 840

Query: 841 EALESTAPLALGGKWDEKLDPAFITNIGQYRRYKYDSVRDLLRVMRNKLNHYRELPKEIQ 900
           +ALESTA +ALG KW+EKL+P FI NIG+YRRYKYDSVRDLLRVMRNKLNHYRELPKEIQ
Sbjct: 841 KALESTAQIALGIKWNEKLEPIFIANIGRYRRYKYDSVRDLLRVMRNKLNHYRELPKEIQ 897

Query: 901 ELIGSVPEGFDNYFASRFPRLLTEVYRVISQYCREEEGFWKYFKSHV 942
           EL+GS+PEGFD+YF +RFP+LL EVY+V S +CR EE F KYFKSHV
Sbjct: 901 ELVGSIPEGFDDYFTTRFPKLLIEVYKVTSCFCRHEECFKKYFKSHV 897

BLAST of Cucsa.101350 vs. NCBI nr
Match: gi|659080670|ref|XP_008440917.1| (PREDICTED: serine/threonine-protein kinase/endoribonuclease IRE1a-like isoform X3 [Cucumis melo])

HSP 1 Score: 1008.1 bits (2605), Expect = 1.0e-290
Identity = 520/809 (64.28%), Postives = 625/809 (77.26%), Query Frame = 1

Query: 138 TKLPRTIDEVVRSTPYIFEDGSVMTGSRKTTVYEVNPVTGKLIRNHSSELSPSGLSNDEF 197
           TKLP TIDEVVR+TPYIFEDG+VMTGSRKTTV+EV+ VTG+LIRNH S+   S LSN+E 
Sbjct: 31  TKLPSTIDEVVRNTPYIFEDGAVMTGSRKTTVFEVDLVTGELIRNHMSKFLSSELSNEE- 90

Query: 198 SVLNGNSSTNKLENRDLIQPGLMKPIEQRLYITRTDYFLKSSFAGSEEVSWSLNVADIGA 257
                  S   ++ +DL+Q  +M P+E RLYITRTDY LKSSF+ SEE SWSLNVA+IGA
Sbjct: 91  --PGSYKSKQNMDIKDLMQSSMMNPVEPRLYITRTDYSLKSSFSNSEEASWSLNVAEIGA 150

Query: 258 TLVCPDGENPTNSVPLDSQNNGSFEFDFTLPLSCQSEVLVYRERSHVLTESSGHKML-SD 317
           TL+CPD ENP   +P   QNN SF  DF +PLSCQS+ LV+R+RSH L+  SG+K L S+
Sbjct: 151 TLLCPDVENPIEGIPWTLQNNNSFGIDFAMPLSCQSKALVFRDRSHFLSGPSGYKRLPSE 210

Query: 318 SHNTDIMLPASASSLMLPSQPSV-KHSNIHPERLMLPGPAANIASLLEPNAISQLNDDSQ 377
           +HN++ +   SAS   LPSQ +V KH N   E+ ML GP               +N+ S 
Sbjct: 211 AHNSNNISLVSASGSFLPSQLTVGKHINTGSEKFMLTGP---------------VNNTSY 270

Query: 378 ALIPMPLMKINDSSAVLGHNIGTTNVDFIAMVLNGPLGLFIALFITMFLGLINRGGALVA 437
            ++P+P MKIN+S+ +    +GT         L G  GLF    +TM +GL+  G  L  
Sbjct: 271 RVVPLPSMKINESNIIQEQKMGT---------LPGAFGLFFVFLLTMLVGLMKYGRTLAV 330

Query: 438 KLKQFLLKEKQPSAVS-SKIVSSKKKKARKLGKNGNFDKKDASASSENEDMV-RSEGDFN 497
           K+KQ  LKE+     S S+++SSKK K RK  K+G+  K++ S SSE EDM+ + E + N
Sbjct: 331 KVKQSFLKEQSSLGTSNSRVISSKKNKPRKSKKSGSSGKREVSISSEIEDMLLQREDNLN 390

Query: 498 NWFPPNNLIDTSGNGRQIGKLMVTNKEIAKGSNGTIVLEGVYEGRLVAVKRLVKTHHDVA 557
           N F  NNL + +G+GR++GKL VTNKEIA GSNGTI+LEG+YEGR VAVKRLVKTHHD+ 
Sbjct: 391 NGFHDNNLTNIAGSGRRVGKLWVTNKEIATGSNGTIILEGIYEGRPVAVKRLVKTHHDIG 450

Query: 558 FKEVQNLIASDRHQNIVRWYGVEYDQDFVYLSLERCTCSLDDLIQICSDPSLNSLLSLDE 617
            KEVQNLI SDRH NIVRWYG++ DQDFVYLSLERCTCSL DLI+I SD  +NS+L LD+
Sbjct: 451 SKEVQNLIVSDRHPNIVRWYGMDSDQDFVYLSLERCTCSLYDLIKIYSDLPMNSMLGLDQ 510

Query: 618 DAGPMIDYKLRLESLKNVISDLNLWKKNSRPAPLLLGLLRDMVAGLEHLHELGIIHRDLK 677
           D G M +Y + LES+K  + +L LW +N RP+ +LL L+RD+VAGLEHLHELGIIHRDLK
Sbjct: 511 DTGRMDEYNIHLESIKVAMPNLKLWNENGRPSSVLLKLMRDIVAGLEHLHELGIIHRDLK 570

Query: 678 PQNVLITKQKSVRAKLSDMGISKRLLKDMSSLGHHATGCGSSGWQAPEQLLHGRQTRAID 737
           PQNVLI KQ+S+ +KLSDMGISKRL  ++SSLGH ATGCGSSGWQAPEQLLHGRQTRAID
Sbjct: 571 PQNVLIVKQQSICSKLSDMGISKRLPANVSSLGHRATGCGSSGWQAPEQLLHGRQTRAID 630

Query: 738 LFSLGCVIFFCLTGGRHPFGDHFERDVNIVRNQMDLFLVEGIPEAMDLISQLLNPNPDLR 797
           LFSLGCV+FFC+TGGRHPFGD  ERDVNIV N+M+L LV+ IPE +DLISQLLNPNP LR
Sbjct: 631 LFSLGCVLFFCVTGGRHPFGDSLERDVNIVNNKMNLLLVDNIPEVVDLISQLLNPNPGLR 690

Query: 798 PRASVVLQHPLFWSSEVRLSFLRDTSDRVELEDRETHSDLLEALESTAPLALGGKWDEKL 857
           P+AS VLQHPLFWS E+RLSFLRDTSDR+ELEDRET  DLL+ALESTA +ALG KW+EKL
Sbjct: 691 PKASEVLQHPLFWSPEMRLSFLRDTSDRIELEDRET--DLLKALESTAQIALGLKWNEKL 750

Query: 858 DPAFITNIGQYRRYKYDSVRDLLRVMRNKLNHYRELPKEIQELIGSVPEGFDNYFASRFP 917
           +P FI NIG+YRRYKYDSVRDLLRVMRNKLNHYRELPKEIQEL+GS+P GFD+YF  RFP
Sbjct: 751 EPIFIANIGRYRRYKYDSVRDLLRVMRNKLNHYRELPKEIQELVGSIPVGFDDYFTRRFP 810

Query: 918 RLLTEVYRVISQYCREEEGFWKYFKSHVE 943
           +LL EVY+VIS +CR+EE F KYFKSHV+
Sbjct: 811 KLLIEVYKVISCFCRQEECFQKYFKSHVD 810