Cucsa.101200 (gene) Cucumber (Gy14) v1 | Cucurbit Genomics Database (CuGenDB)

Overview


Name	Cucsa.101200
Type	gene
Organism	Cucumis sativus (Cucumber (Gy14) v1)
Description	WAT1-related protein
Location	scaffold00927 : 458655 .. 461021 (+)

Genome Viewer

Gene Expression

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGAATTCAAAGAAGCCATATTTTGCTGCACTCCTTTGTCAAACAATATTTGCAGGGATGAGTTTACTCTCAAAAGCTTCCTATTCTTCGGGTATGAATACTTTTATTTTCTTTTTCTACCGGCAAGCGGTTGGCACTATCTTCTTACTTCCCCTCACCATTTATTTCTCAAGGTAAAATACTCCATATTATTACTAAATTCATTTCCTAACTCCTTCTATCCTTTAATTAACTACAATTGTGTACTCATGAGTCTGTAGTCATTCTTTTTTGGAGAAACCAAAACCAATTGAGAAAATAGCTAAAAAAAaCAGATCTAACCGATTTGTTTGTTTTTGTTTTCATGGATGATTTCTGTAGAAATGAAATGGCAAGTTTATCAAAAGGAGATCTGTTGAAAATTTTCATGCAGGCCTTCTTAGGGTTTGTGCCATTAATTATATATTGCAAAGTTGTTGAATTCTCTTAATTTTATTAAGATTATATTTAATTTGATTGAAAATTTTACTTGCGTAGATGAAATTAAAGTGCAGGTTTACGTTTGGTTTTAATGCTTATGGATTAGGGGTTAAGTATACATCCGCCACCTTAGGAGCTGCTGCATTTAATTGTATTCCTGTCACCACGTTCTTCTTTGCTTTTATTTTAAGGTTTGTTAACATTATGTTATTTGAATTTATTTACTTTTCTCCTAAATAATTTAAAGAAACAGTTCAAAACTATATCTTAATAAAAGTTATAGGCAAACTAACAAAACTCATCATCATGTAGGTCTCTGCTTTTTTAAAATTAAGGTATCTTTGTTTATATTTTTGTTTTACTTTTGAAAATTTGTTTAATTCACCCTCGTTAAGATACTTCAAAATTCAAAGAATATGTTCTGAGAACTACAAAGAGTAAGTTTGTTATATTTCTTTTTATTTTTTtAATTAAGAAATCAAGTGCTACTTAAGAAAATATATAAAAATCATTATATAAAGAACGGTGAGAAAAATAGGCATAATTTTTAAAATAGAAATGATTACAAGATGAATTTAAATTCCTAGTTTTTtGAAATTGGAATTTAGAATTTGGCAAATCGTATTGGTAAAAAGTAGTAATTCTGATTAACCATCCAAAATGGGTAAGAAGTTTATACCAAATGCACTACTTTAATTAAATCAATAACTTCTAAAAGAAAATAATAGAATATCTAATTGGTTTGGTTATTCAAGCCCTGAATAATGCAACTTGTATGGATAAATTATTCATAAATTACTAGTTGAAGTGAATGAAGATAAAATAACATTTAAGAAGTTTGAACTCAAGATCTCCAGTATGGACTAACCTACTCTAGTATCATTTTAATTGATCCATAAACATAAACTCTATCGATATATATTTGGTATTGATAGGGTGGAGAAAGTAAATACAAAGAAAGCAAGTGGAATGGCAAAGGTTGGAGGCATAATGTTGTGCATTACTGGTGTTGCGGTTATTACATTTTACAAAGGTCCTTATTTAAAACCACTCAACAACGTTCACCTTTTTCAAACCCAACAAGCTCATGTTTCTTCCAAAAAaGAATGGATTCTTGGCTGTTCCTTGCTCCTCCTTTCTTCCCTTGCAGTGGGTTTGTGGTTTGTTCTCCAGGTTAATTTTCAACATTATCATGAATCATGTGTGGTTTATGTTATTGTTGTATGAGTAATCCTTTGATATAATATTTTATTTTCAGGTTTGGGTTTTGAGAACTTGTCCATCTCCACTTGTAGTAACATTTGGACAGACATTTTCAAGTAGCATTCAGTCCTTTGTTGTGGCTATTGCAATCGAAAGAAACCCATCTCAATGGAAGCTGGCTTGGAACATTTCCCTAGCTGCAATTCTTTATTGTGTATGATTTCTTATTTAACAATATACGGAGAATGCGGATTTGAACCTCTAGCTAACTATTAATTTGAGTCATTCAAATAGATAGCACTTATCGATTACTTCTAAACTATAATCTTCTTTTATTGTGCAGGGAGTTTTTGTAGTTTCCATTGGAAACTATTTGTCAAGCTGGGTGGTTAAGAAAAAGGGTCCAGTTTTTCAAGCTGTTACTACACCCTTCAATCTCATTGTTACACTTATTGGTTCAGAATTTCTTTTTAAGTATGGAATTTGCTTGGGAAGGTAATTATGAAATTAATTGATGGATTATTCATTGTTAATGATTTAAAATTGTTTAAACCAAATATTTGACTTTTTTGTTTTTGGTGGGTGAACAGCGGAATTGGTGCTATATTGCTAGTTTTGAGTCTTTATAGCGTTTTATGGGGAAAAAAGAAGGAAGCATGTTGTCATGATGCATCAAACAACAACAGCATCACCAATTCTGTTCAG

mRNA sequence

ATGAATTCAAAGAAGCCATATTTTGCTGCACTCCTTTGTCAAACAATATTTGCAGGGATGAGTTTACTCTCAAAAGCTTCCTATTCTTCGGGTATGAATACTTTTATTTTCTTTTTCTACCGGCAAGCGGTTGGCACTATCTTCTTACTTCCCCTCACCATTTATTTCTCAAGAAATGAAATGGCAAGTTTATCAAAAGGAGATCTGTTGAAAATTTTCATGCAGGCCTTCTTAGGGTTTTGCAGGTTTACGTTTGGTTTTAATGCTTATGGATTAGGGGTTAAGTATACATCCGCCACCTTAGGAGCTGCTGCATTTAATTGTATTCCTGTCACCACGTTCTTCTTTGCTTTTATTTTAAGGGTGGAGAAAGTAAATACAAAGAAAGCAAGTGGAATGGCAAAGGTTGGAGGCATAATGTTGTGCATTACTGGTGTTGCGGTTATTACATTTTACAAAGGTCCTTATTTAAAACCACTCAACAACGTTCACCTTTTTCAAACCCAACAAGCTCATGTTTCTTCCAAAAAAGAATGGATTCTTGGCTGTTCCTTGCTCCTCCTTTCTTCCCTTGCAGTGGGTTTGTGGTTTGTTCTCCAGGTTTGGGTTTTGAGAACTTGTCCATCTCCACTTGTAGTAACATTTGGACAGACATTTTCAAGTAGCATTCAGTCCTTTGTTGTGGCTATTGCAATCGAAAGAAACCCATCTCAATGGAAGCTGGCTTGGAACATTTCCCTAGCTGCAATTCTTTATTGTGGAGTTTTTGTAGTTTCCATTGGAAACTATTTGTCAAGCTGGGTGGTTAAGAAAAAGGGTCCAGTTTTTCAAGCTGTTACTACACCCTTCAATCTCATTGTTACACTTATTGTTTTGAGTCTTTATAGCGTTTTATGGGGAAAAAAGAAGGAAGCATGTTGTCATGATGCATCAAACAACAACAGCATCACCAATTCTGTTCAG

Coding sequence (CDS)

ATGAATTCAAAGAAGCCATATTTTGCTGCACTCCTTTGTCAAACAATATTTGCAGGGATGAGTTTACTCTCAAAAGCTTCCTATTCTTCGGGTATGAATACTTTTATTTTCTTTTTCTACCGGCAAGCGGTTGGCACTATCTTCTTACTTCCCCTCACCATTTATTTCTCAAGAAATGAAATGGCAAGTTTATCAAAAGGAGATCTGTTGAAAATTTTCATGCAGGCCTTCTTAGGGTTTTGCAGGTTTACGTTTGGTTTTAATGCTTATGGATTAGGGGTTAAGTATACATCCGCCACCTTAGGAGCTGCTGCATTTAATTGTATTCCTGTCACCACGTTCTTCTTTGCTTTTATTTTAAGGGTGGAGAAAGTAAATACAAAGAAAGCAAGTGGAATGGCAAAGGTTGGAGGCATAATGTTGTGCATTACTGGTGTTGCGGTTATTACATTTTACAAAGGTCCTTATTTAAAACCACTCAACAACGTTCACCTTTTTCAAACCCAACAAGCTCATGTTTCTTCCAAAAAaGAATGGATTCTTGGCTGTTCCTTGCTCCTCCTTTCTTCCCTTGCAGTGGGTTTGTGGTTTGTTCTCCAGGTTTGGGTTTTGAGAACTTGTCCATCTCCACTTGTAGTAACATTTGGACAGACATTTTCAAGTAGCATTCAGTCCTTTGTTGTGGCTATTGCAATCGAAAGAAACCCATCTCAATGGAAGCTGGCTTGGAACATTTCCCTAGCTGCAATTCTTTATTGTGGAGTTTTTGTAGTTTCCATTGGAAACTATTTGTCAAGCTGGGTGGTTAAGAAAAAGGGTCCAGTTTTTCAAGCTGTTACTACACCCTTCAATCTCATTGTTACACTTATTGTTTTGAGTCTTTATAGCGTTTTATGGGGAAAAAAGAAGGAAGCATGTTGTCATGATGCATCAAACAACAACAGCATCACCAATTCTGTTCAG

Protein sequence

MNSKKPYFAALLCQTIFAGMSLLSKASYSSGMNTFIFFFYRQAVGTIFLLPLTIYFSRNEMASLSKGDLLKIFMQAFLGFCRFTFGFNAYGLGVKYTSATLGAAAFNCIPVTTFFFAFILRVEKVNTKKASGMAKVGGIMLCITGVAVITFYKGPYLKPLNNVHLFQTQQAHVSSKKEWILGCSLLLLSSLAVGLWFVLQVWVLRTCPSPLVVTFGQTFSSSIQSFVVAIAIERNPSQWKLAWNISLAAILYCGVFVVSIGNYLSSWVVKKKGPVFQAVTTPFNLIVTLIVLSLYSVLWGKKKEACCHDASNNNSITNSVQ

Homology

[Close]

BLAST of Cucsa.101200 vs. Swiss-Prot
Match: WTR45_ARATH (WAT1-related protein At5g64700 OS=Arabidopsis thaliana GN=At5g64700 PE=2 SV=1)

HSP 1 Score: 256.5 bits (654), Expect = 3.8e-67
Identity = 139/333 (41.74%), Postives = 187/333 (56.16%), Query Frame = 1


Query: 1   MNSKKPYFAALLCQTIFAGMSLLSKASYSSGMNTFIFFFYRQAVGTIFLLPLTIYFSRNE 60
           M SKKPY    + Q I+  M L+SKA ++ GMNTF+F FYRQA  TIFL PL  +F R  
Sbjct: 3   MESKKPYLMVTIIQVIYTIMFLISKAVFNGGMNTFVFVFYRQAFATIFLAPLAFFFERKS 62

Query: 61  MASLSKGDLLKIFMQAFLGFCRFTFGFNAYGLGVKYTSATLGAAAFNCIPVTTFFFAFIL 120
              LS    +KIFM +  G    T   +  G+ + YTSATL AA    +P  TFF A + 
Sbjct: 63  APPLSFVTFIKIFMLSLFGV---TLSLDLNGIALSYTSATLAAATTASLPAITFFLALLF 122

Query: 121 RVEKVNTKKASGMAKVGGIMLCITGVAVITFYKGPYLKPLNNVHLFQTQQ-------AHV 180
            +E++  K   G AK+ GI +C+ GV ++  YKGP LK     H +  Q+        HV
Sbjct: 123 GMERLKVKSIQGTAKLVGITVCMGGVIILAIYKGPLLKLPLCPHFYHGQEHPHRNNPGHV 182

Query: 181 SS-KKEWILGCSLLLLSSLAVGLWFVLQVWVLRTCPSPLVVTFGQTFSSSIQSFVVAIAI 240
           S     W+ GC L++ S++  GLW VLQ  VL+  PS L  T      SSIQSFV+AIA+
Sbjct: 183 SGGSTSWLKGCVLMITSNILWGLWLVLQGRVLKVYPSKLYFTTLHCLLSSIQSFVIAIAL 242

Query: 241 ERNPSQWKLAWNISLAAILYCGVFVVSIGNYLSSWVVKKKGPVFQAVTTPFNLIVT---- 300
           ER+ S WKL WN+ L A++YCG  V  +  YL SWV++K+GPVF ++ TP +L+ T    
Sbjct: 243 ERDISAWKLGWNLRLVAVIYCGFIVTGVAYYLQSWVIEKRGPVFLSMFTPLSLLFTLLSS 302

Query: 301 -----------------LIVLSLYSVLWGKKKE 305
                            L+++ LY VLWGK +E
Sbjct: 303 AILLCEIISLGSIVGGLLLIIGLYCVLWGKSRE 332

[Close]

BLAST of Cucsa.101200 vs. Swiss-Prot
Match: WTR7_ARATH (WAT1-related protein At1g43650 OS=Arabidopsis thaliana GN=At1g43650 PE=2 SV=1)

HSP 1 Score: 200.3 bits (508), Expect = 3.2e-50
Identity = 119/325 (36.62%), Postives = 168/325 (51.69%), Query Frame = 1


Query: 1   MNSKKPYFAALLCQTIFAGMSLLSKASYSSGMNTFIFFFYRQAVGTIFLLPLTIYFSRNE 60
           M   K   A +  Q ++AGM LLSK + S G N F+F FYRQA   + L P   +   ++
Sbjct: 2   MMEHKANMAMVFVQIVYAGMPLLSKVAISQGTNPFVFVFYRQAFAALALSPFAFFLESSK 61

Query: 61  MASLSKGDLLKIFMQAFLGFCRFTFGFNAYGLGVKYTSATLGAAAFNCIPVTTFFFAFIL 120
            + LS   LLKIF   F+  C  T   N Y + ++ T+AT  AA  N IP  TF  A + 
Sbjct: 62  SSPLSFILLLKIF---FISLCGLTLSLNLYYVAIENTTATFAAATTNAIPSITFVLALLF 121

Query: 121 RVEKVNTKKASGMAKVGGIMLCITGVAVITFYKGPYLKPLNNVHLFQTQQAHVSSKKEWI 180
           R+E V  KK+ G+AKV G M+ + G  V  F KGP L  +N+ +        V S K  +
Sbjct: 122 RLETVTLKKSHGVAKVTGSMVGMLGALVFAFVKGPSL--INHYNSSTIPNGTVPSTKNSV 181

Query: 181 LGCSLLLLSSLAVGLWFVLQVWVLRTCPSPLVVTFGQTFSSSIQSFVVAIAIERNPSQWK 240
            G   +L ++    LW ++Q  V++  P+ L +   Q   S IQS V A+A+ RNPS WK
Sbjct: 182 KGSITMLAANTCWCLWIIMQSKVMKEYPAKLRLVALQCLFSCIQSAVWAVAVNRNPSVWK 241

Query: 241 LAWNISLAAILYCGVFVVSIGNYLSSWVVKKKGPVFQAVTTPFNLIVT------------ 300
           + + + L ++ YCG+ V  +  +L  W ++KKGPVF A+ TP  LI+T            
Sbjct: 242 IEFGLPLLSMAYCGIMVTGLTYWLQVWAIEKKGPVFTALYTPLALILTCIVSSFLFKETF 301

Query: 301 ---------LIVLSLYSVLWGKKKE 305
                    L+V  LY  LWGK KE
Sbjct: 302 YLGSVGGAVLLVCGLYLGLWGKTKE 321

[Close]

BLAST of Cucsa.101200 vs. Swiss-Prot
Match: WTR38_ARATH (WAT1-related protein At5g07050 OS=Arabidopsis thaliana GN=At5g07050 PE=2 SV=1)

HSP 1 Score: 191.8 bits (486), Expect = 1.1e-47
Identity = 116/357 (32.49%), Postives = 182/357 (50.98%), Query Frame = 1


Query: 1   MNSKKPYFAALLCQTIFAGMSLLSKASYSSGMNTFIFFFYRQAVGTIFLLPLTIYFSRNE 60
           + S KPYFA +  Q  +AGM++++K S ++GM+ ++   YR A+ T  + P   +F R  
Sbjct: 13  LTSSKPYFAMISLQFGYAGMNIITKISLNTGMSHYVLVVYRHAIATAVIAPFAFFFERKA 72

Query: 61  MASLSKGDLLKIFMQAF-LGFCRFTFGFNAYGLGVKYTSATLGAAAFNCIPVTTFFFAFI 120
              ++      IFMQ F LG        N Y +G+KYTS T   A  N +P  TF  A +
Sbjct: 73  QPKIT----FSIFMQLFILGLLGPVIDQNFYYMGLKYTSPTFSCAMSNMLPAMTFILAVL 132

Query: 121 LRVEKVNTKKASGMAKVGGIMLCITGVAVITFYKGP--------YLKPLNNVHLFQTQQA 180
            R+E ++ KK    AK+ G ++ + G  ++T YKGP        Y+   ++ H   T   
Sbjct: 133 FRMEMLDLKKLWCQAKIAGTVVTVAGAMLMTIYKGPIVELFWTKYMHIQDSSHANTTSSK 192

Query: 181 HVSSKKEWILGCSLLLLSSLAVGLWFVLQVWVLRT-CPSPLVVTFGQTFSSSIQSFVVAI 240
           + SS KE++ G  LL+ ++LA    FVLQ  +L+T     L +T    F  ++Q+  V  
Sbjct: 193 NSSSDKEFLKGSILLIFATLAWASLFVLQAKILKTYAKHQLSLTTLICFIGTLQAVAVTF 252

Query: 241 AIERNPSQWKLAWNISLAAILYCGVFVVSIGNYLSSWVVKKKGPVFQAVTTPFNLIV--- 300
            +E NPS W++ W+++L A  Y G+   SI  Y+   V+KK+GPVF    +P  +++   
Sbjct: 253 VMEHNPSAWRIGWDMNLLAAAYSGIVASSISYYVQGIVMKKRGPVFATAFSPLMMVIVAV 312

Query: 301 ------------------TLIVLSLYSVLWGKKKE---ACCHDA--SNNNSITNSVQ 322
                              LIV+ LY+VLWGK+KE     C  A   +N+ +T  V+
Sbjct: 313 MGSFVLAEKIFLGGVIGAVLIVIGLYAVLWGKQKENQVTICELAKIDSNSKVTEDVE 365

[Close]

BLAST of Cucsa.101200 vs. Swiss-Prot
Match: WTR14_ARATH (WAT1-related protein At2g39510 OS=Arabidopsis thaliana GN=At2g39510 PE=2 SV=1)

HSP 1 Score: 169.1 bits (427), Expect = 8.0e-41
Identity = 105/323 (32.51%), Postives = 159/323 (49.23%), Query Frame = 1


Query: 5   KPYFAALLCQTIFAGMSLLSKASYSSGMNTFIFFFYRQAVGTIFLLPLTIYFSRNEMASL 64
           KP+   +  Q  +AG+S+++K + + GM+  +   YR  V TIF+ P   +  R     +
Sbjct: 7   KPFITVVSLQFGYAGLSIIAKFALNQGMSPHVLASYRHIVATIFIAPFAYFLDRKIRPKM 66

Query: 65  SKGDLLKIFMQAFLGFCRFTFGFNAYGLGVKYTSATLGAAAFNCIPVTTFFFAFILRVEK 124
           +     KI +   LG    T   N Y  G+KYTSAT  AA  N +P   F  A+I R+EK
Sbjct: 67  TLSIFFKILL---LGLLEPTIDQNLYYTGMKYTSATFTAAMTNVLPAFAFIMAWIFRLEK 126

Query: 125 VNTKKASGMAKVGGIMLCITGVAVITFYKGPYLK-PLNNVHLFQTQQAHVSSKKEWILGC 184
           VN KK    AK+ G ++ + G  ++T  KGP +  P  N H      ++   K++   G 
Sbjct: 127 VNVKKIHSQAKILGTIVTVGGAMLMTVVKGPLIPLPWANPHDIHQDSSNTGVKQDLTKGA 186

Query: 185 SLLLLSSLAVGLWFVLQVWVLRTCPSPLVVTFGQTFSSSIQSFVVAIAIER-NPSQWKLA 244
           SL+ +  +    +  LQ   L++ P  L +T    F  SI+S +VA+ IER NPS W + 
Sbjct: 187 SLIAIGCICWAGFINLQAITLKSYPVELSLTAYICFLGSIESTIVALFIERGNPSAWAIH 246

Query: 245 WNISLAAILYCGVFVVSIGNYLSSWVVKKKGPVFQAVTTPFNLIVT-------------- 304
            +  L A +Y GV    IG Y+   ++K +GPVF     P ++++               
Sbjct: 247 LDSKLLAAVYGGVICSGIGYYVQGVIMKTRGPVFVTAFNPLSMVIVAILGSIILAEVMFL 306

[Close]

BLAST of Cucsa.101200 vs. Swiss-Prot
Match: WTR37_ARATH (WAT1-related protein At4g30420 OS=Arabidopsis thaliana GN=At4g30420 PE=3 SV=1)

HSP 1 Score: 166.8 bits (421), Expect = 3.9e-40
Identity = 95/335 (28.36%), Postives = 162/335 (48.36%), Query Frame = 1


Query: 9   AALLCQTIFAGMSLLSKASYSSGMNTFIFFFYRQAVGTIFLLPLTIYFSRNEMASLSKGD 68
           A  + Q  +AG++L ++A+   G++  +F  YRQA  TIF+ P      R    ++S  D
Sbjct: 2   AMTMIQLCYAGVTLFARATLVHGLSPRVFILYRQAFATIFIFPFLYLSRRKSKIAISSLD 61

Query: 69  LLKIFMQAFLGFCRFTFGFNAYGLGVKYTSATLGAAAFNCIPVTTFFFAFILRVEKVNTK 128
           L    +   +     T   N Y  G+  TS+++G+A  N IP  TF  +F+   EK+N +
Sbjct: 62  LKSFSLIFLVSLIGITINQNLYLEGLYLTSSSMGSAVGNIIPAITFLISFLAGYEKLNLR 121

Query: 129 KASGMAKVGGIMLCITGVAVITFYKGP-YLKPLNNVHLFQTQQAHVSSKKEWILGCSLLL 188
              G+AK+ G +LC+ G   +T  +GP  L   + + + ++   H+  +  W++GC  L 
Sbjct: 122 DIRGLAKIAGTILCVAGAISMTLLRGPKILNSESALPIAKSVLGHLKDQNTWLIGCLFLF 181

Query: 189 LSSLAVGLWFVLQVWVLRTCPSPLVVTFGQTFSSSIQSFVVAIAIERNPSQWKLAWNISL 248
            S+L    W +LQV +    P  L ++       +IQ  VV   +E++P+ W L      
Sbjct: 182 SSTLCWSFWLILQVPISAYYPDNLSLSAWMCLFGTIQCAVVTFFLEKDPNAWILHSYSEF 241

Query: 249 AAILYCGVFVVSIGNYLSSWVVKKKGPVFQAVTTPF-NLIVTL----------------- 308
           A  LY G+   ++   + +W + K+GPVF A+  P   +IVT+                 
Sbjct: 242 ATCLYAGIGASALSFTVQAWAIAKRGPVFSALFNPLCTVIVTILAALFFHEEIYTGSLIG 301

Query: 309 ---IVLSLYSVLWGKKKEACCHDASNNNSITNSVQ 322
              ++L LY+VLWGK K+   +    +N   + V+
Sbjct: 302 GLGVILGLYTVLWGKAKDVMMNQDQRDNDQKSEVK 336

[Close]

BLAST of Cucsa.101200 vs. TrEMBL
Match: A0A0A0KF97_CUCSA (WAT1-related protein OS=Cucumis sativus GN=Csa_6G485120 PE=3 SV=1)

HSP 1 Score: 617.8 bits (1592), Expect = 7.2e-174
Identity = 318/342 (92.98%), Postives = 318/342 (92.98%), Query Frame = 1


Query: 1   MNSKKPYFAALLCQTIFAGMSLLSKASYSSGMNTFIFFFYRQAVGTIFLLPLTIYFSRNE 60
           MNSKKPYFAALLCQTIFAGMSLLSKASYSSGMNTFIFFFYRQAVGTIFLLPLTIYFSRNE
Sbjct: 5   MNSKKPYFAALLCQTIFAGMSLLSKASYSSGMNTFIFFFYRQAVGTIFLLPLTIYFSRNE 64

Query: 61  MASLSKGDLLKIFMQAFLGFCRFTFGFNAYGLGVKYTSATLGAAAFNCIPVTTFFFAFIL 120
           MASLSKGDLLKIFMQAFLG   FTFGFNAYGLGVKYTSATLGAAAFNCIPVTTFFFAFIL
Sbjct: 65  MASLSKGDLLKIFMQAFLG---FTFGFNAYGLGVKYTSATLGAAAFNCIPVTTFFFAFIL 124

Query: 121 RVEKVNTKKASGMAKVGGIMLCITGVAVITFYKGPYLKPLNNVHLFQTQQAHVSSKKEWI 180
           RVEKVNTKKASGMAKVGGIMLCITGVAVITFYKGPYLKPLNNVHLFQTQQAHVSSKKEWI
Sbjct: 125 RVEKVNTKKASGMAKVGGIMLCITGVAVITFYKGPYLKPLNNVHLFQTQQAHVSSKKEWI 184

Query: 181 LGCSLLLLSSLAVGLWFVLQVWVLRTCPSPLVVTFGQTFSSSIQSFVVAIAIERNPSQWK 240
           LGCSLLLLSSLAVGLWFVLQVWVLRTCPSPLVVTFGQTFSSSIQSFVVAIAIERNPSQWK
Sbjct: 185 LGCSLLLLSSLAVGLWFVLQVWVLRTCPSPLVVTFGQTFSSSIQSFVVAIAIERNPSQWK 244

Query: 241 LAWNISLAAILYCGVFVVSIGNYLSSWVVKKKGPVFQAVTTPFNLIVTLI---------- 300
           LAWNISLAAILYCGVFVVSIGNYLSSWVVKKKGPVFQAVTTPFNLIVTLI          
Sbjct: 245 LAWNISLAAILYCGVFVVSIGNYLSSWVVKKKGPVFQAVTTPFNLIVTLIGSEFLFKYGI 304

Query: 301 -----------VLSLYSVLWGKKKEACCHDASNNNSITNSVQ 322
                      VLSLYSVLWGKKKEACCHDASNNNSITNSVQ
Sbjct: 305 CLGSGIGAILLVLSLYSVLWGKKKEACCHDASNNNSITNSVQ 343

[Close]

BLAST of Cucsa.101200 vs. TrEMBL
Match: A0A0A0KHU9_CUCSA (WAT1-related protein OS=Cucumis sativus GN=Csa_6G485110 PE=3 SV=1)

HSP 1 Score: 469.2 bits (1206), Expect = 4.1e-129
Identity = 234/318 (73.58%), Postives = 259/318 (81.45%), Query Frame = 1


Query: 20  MSLLSKASYSSGMNTFIFFFYRQAVGTIFLLPLTIYFSRNEMASLSKGDLLKIFMQAFLG 79
           MSL SKA++ SGMNT+IF FYRQA G+I LLPLTIYF+R EM SLSK D++KIF+Q  LG
Sbjct: 1   MSLFSKAAFVSGMNTYIFLFYRQAFGSIVLLPLTIYFARKEMRSLSKADVMKIFLQGLLG 60

Query: 80  FCRFTFGFNAYGLGVKYTSATLGAAAFNCIPVTTFFFAFILRVEKVNTKKASGMAKVGGI 139
           F   T G N YGLGVKYTSATLGAAAFNC+PVTTFFFA + R+EKVN +KASGMAKVGGI
Sbjct: 61  F---TIGLNVYGLGVKYTSATLGAAAFNCVPVTTFFFALLSRMEKVNIRKASGMAKVGGI 120

Query: 140 MLCITGVAVITFYKGPYLKPLNNVHLFQTQQAHVSSKKEWILGCSLLLLSSLAVGLWFVL 199
           +LCI GVAV+ FYKGPYLKPL N HLFQTQQ+HVSSKKEWILGC LL L+ L  GLW+VL
Sbjct: 121 ILCIAGVAVLAFYKGPYLKPLFNFHLFQTQQSHVSSKKEWILGCFLLFLTCLTWGLWYVL 180

Query: 200 QVWVLRTCPSPLVVTFGQTFSSSIQSFVVAIAIERNPSQWKLAWNISLAAILYCGVFVVS 259
           Q WVLR+CPSPLV+TFGQTFSS+IQSFVVAIAIERNPS+WKL WNISLAAILYCG FV+ 
Sbjct: 181 QAWVLRSCPSPLVLTFGQTFSSAIQSFVVAIAIERNPSEWKLGWNISLAAILYCGTFVIP 240

Query: 260 IGNYLSSWVVKKKGPVFQAVTTPFNLIVTLI---------------------VLSLYSVL 317
           +GNYLSSWVVKKKGPVFQAVTTPFNLI TLI                     VLSLYSVL
Sbjct: 241 VGNYLSSWVVKKKGPVFQAVTTPFNLIFTLIGSEFLLNDGISLGSIIGAILLVLSLYSVL 300

[Close]

BLAST of Cucsa.101200 vs. TrEMBL
Match: A0A0A0KFR8_CUCSA (WAT1-related protein OS=Cucumis sativus GN=Csa_6G484610 PE=3 SV=1)

HSP 1 Score: 375.2 bits (962), Expect = 8.1e-101
Identity = 197/350 (56.29%), Postives = 242/350 (69.14%), Query Frame = 1


Query: 1   MNSKKPYFAALLCQTIFAGMSLLSKASYSSGMNTFIFFFYRQAVGTIFLLPLTIYFSRNE 60
           M  KKP   A++ Q  ++GMS+L+KA+++SGMN FIF FYRQA GT+FL+P TI F R E
Sbjct: 1   MELKKPLIGAIVVQITYSGMSILAKAAFTSGMNNFIFIFYRQAFGTLFLIPPTILFKRKE 60

Query: 61  MASLSKGDLLKIFMQAFLGFCRFTFGFNAYGLGVKYTSATLGAAAFNCIPVTTFFFAFIL 120
           +A LS GD+ KIFM A LG    T    AYGLGVKYTSA  GAAAFN +PVTTF FA +L
Sbjct: 61  VACLSIGDMFKIFMLALLGR---TLVLIAYGLGVKYTSAVSGAAAFNALPVTTFLFALLL 120

Query: 121 RVEKVNTKKASGMAKVGGIMLCITGVAVITFYKGPYLKPLNNVHLFQT---QQAHVSSKK 180
           R+EK+  KKASGMAKVGG+MLC+ GV+++ FYKGP++KPL N HL +T      H S+  
Sbjct: 121 RMEKLKVKKASGMAKVGGLMLCVVGVSILAFYKGPFMKPLFNFHLLETPHHNNPHPSNSS 180

Query: 181 E------WILGCSLLLLSSLAVGLWFVLQVWVLR-TCPSPLVVTFGQTFSSSIQSFVVAI 240
                  W LGC +LL+SS+  GLW VLQ  VL+ +CPSPLV+T GQT SS+ Q+FVVAI
Sbjct: 181 PQPPQHTWALGCFMLLVSSICSGLWLVLQALVLKHSCPSPLVLTCGQTLSSAFQTFVVAI 240

Query: 241 AIERNPSQWKLAWNISLAAILYCGVFVVSIGNYLSSWVVKKKGPVFQAVTTPFNLIVTLI 300
           A+E NPS+WKL WNI L ++LYCG+FV+  GNYL+ WV+KKKGPVF A TTP NLI TLI
Sbjct: 241 AVESNPSEWKLGWNIRLFSVLYCGIFVICTGNYLACWVIKKKGPVFLAATTPLNLIATLI 300

Query: 301 ---------------------VLSLYSVLWGKKKEACCHDASNNNSITNS 320
                                VLSLYSVLWG+ KE  C + +  N I NS
Sbjct: 301 ASQFLLTDGTSLGSLIGGTLLVLSLYSVLWGQSKEKDCEN-TQINLINNS 346

[Close]

BLAST of Cucsa.101200 vs. TrEMBL
Match: A0A0A0KJ33_CUCSA (WAT1-related protein OS=Cucumis sativus GN=Csa_6G485670 PE=3 SV=1)

HSP 1 Score: 328.9 bits (842), Expect = 6.7e-87
Identity = 162/329 (49.24%), Postives = 218/329 (66.26%), Query Frame = 1


Query: 1   MNSKKPYFAALLCQTIFAGMSLLSKASYSSGMNTFIFFFYRQAVGTIFLLPLTIYFSRNE 60
           M+SKKPY  A+  Q  FAGMSLLSKA++++GMNT+IF FYRQA G++ L+PLT+     E
Sbjct: 1   MDSKKPYLVAIFIQITFAGMSLLSKAAFATGMNTYIFLFYRQAAGSLILIPLTLLLKGKE 60

Query: 61  MASLSKGDLLKIFMQAFLGFCRFTFGFNAYGLGVKYTSATLGAAAFNCIPVTTFFFAFIL 120
              LS   L + F  + +G    T   NAYG+ V YTSATLGAAAFNC+PV+TF FA + 
Sbjct: 61  KRPLSFKQLCQCFFISLIGI---TLAMNAYGVAVDYTSATLGAAAFNCLPVSTFIFAVLF 120

Query: 121 RVEKVNTKKASGMAKVGGIMLCITGVAVITFYKGPYLKPLNNVHLFQTQQAHV----SSK 180
           R+E+VN KKA+G+AKVGG+M+C+ G A++ FYKGPYLKP+ +  +F  +++      +S+
Sbjct: 121 RMERVNLKKAAGIAKVGGMMICVGGAAILAFYKGPYLKPIISHPIFHIEESETDITTTSQ 180

Query: 181 KEWILGCSLLLLSSLAVGLWFVLQVWVLRTCPSPLVVTFGQTFSSSIQSFVVAIAIERNP 240
           K W+LGC  LL++++  G+WFV Q   L+  P P+     QT  S +Q FVVAI +ER+P
Sbjct: 181 KSWLLGCFFLLVATVGWGIWFVFQAKFLKGYPHPVEFMCAQTVMSVVQCFVVAIIVERDP 240

Query: 241 SQWKLAWNISLAAILYCGVFVVSIGNYLSSWVVKKKGPVFQAVTTPFNLIVT-------- 300
           S+WKL WN+ L A+LYCG+ V+ I N    WV+K+KGPVFQA+  P NL+ T        
Sbjct: 241 SEWKLGWNVRLYAVLYCGILVIGIANNAQCWVIKEKGPVFQAMMMPLNLVATIIGSQLFL 300

Query: 301 -------------LIVLSLYSVLWGKKKE 305
                        L+V SLYSVLWGK KE
Sbjct: 301 AEGIYLGSVIGAILLVTSLYSVLWGKNKE 326

[Close]

BLAST of Cucsa.101200 vs. TrEMBL
Match: A0A0A0KFA3_CUCSA (WAT1-related protein OS=Cucumis sativus GN=Csa_6G485160 PE=3 SV=1)

HSP 1 Score: 325.1 bits (832), Expect = 9.7e-86
Identity = 165/329 (50.15%), Postives = 219/329 (66.57%), Query Frame = 1


Query: 1   MNSKKPYFAALLCQTIFAGMSLLSKASYSSGMNTFIFFFYRQAVGTIFLLPLTIYFSRNE 60
           M S KPY      Q  FAGMSL+SKA++++GMNT+IF FYRQA G+I L+PLT+     E
Sbjct: 1   MGSNKPYIVVFFIQITFAGMSLMSKAAFAAGMNTYIFLFYRQAAGSIILVPLTLILKGKE 60

Query: 61  MASLSKGDLLKIFMQAFLGFCRFTFGFNAYGLGVKYTSATLGAAAFNCIPVTTFFFAFIL 120
              LS   L +IF+ + +G    T   +AYG+ + YTSAT+GAAAFNC+PVTTFFFA +L
Sbjct: 61  KRPLSLKHLCQIFVISLIGI---TLALDAYGVAINYTSATIGAAAFNCVPVTTFFFAVLL 120

Query: 121 RVEKVNTKKASGMAKVGGIMLCITGVAVITFYKGPYLKPLNNVHLFQ---TQQAH-VSSK 180
           R+EKVN KKA+G+AKV GIM+CITG +++  YKGPYLKPL    LF    +Q  H ++S 
Sbjct: 121 RMEKVNLKKAAGIAKVVGIMICITGASILALYKGPYLKPLFTHQLFHHIHSQPHHSLNSH 180

Query: 181 KEWILGCSLLLLSSLAVGLWFVLQVWVLRTCPSPLVVTFGQTFSSSIQSFVVAIAIERNP 240
            +W++GC  LL++S++ G+WFVLQ   L+  P P+     QT  S +QSF+VAI +ER+P
Sbjct: 181 TKWMIGCFFLLVTSISWGIWFVLQARFLKGYPHPMEFMCMQTLMSMVQSFIVAIVMERDP 240

Query: 241 SQWKLAWNISLAAILYCGVFVVSIGNYLSSWVVKKKGPVFQAVTTPFNLIVT-------- 300
            +WKL +NI L A+LYCG+ V+ + N    WV+++KGPVFQA+TTP NLI T        
Sbjct: 241 FEWKLGFNIRLFAVLYCGILVIGVANNAQCWVIREKGPVFQAMTTPLNLIATIIGSQFLF 300

Query: 301 -------------LIVLSLYSVLWGKKKE 305
                        L+V SLY VLWGK KE
Sbjct: 301 PDGTYLGSIIGAFLLVTSLYCVLWGKNKE 326

[Close]

BLAST of Cucsa.101200 vs. TAIR10
Match: AT5G64700.1 (AT5G64700.1 nodulin MtN21 /EamA-like transporter family protein)

HSP 1 Score: 256.5 bits (654), Expect = 2.1e-68
Identity = 139/333 (41.74%), Postives = 187/333 (56.16%), Query Frame = 1


Query: 1   MNSKKPYFAALLCQTIFAGMSLLSKASYSSGMNTFIFFFYRQAVGTIFLLPLTIYFSRNE 60
           M SKKPY    + Q I+  M L+SKA ++ GMNTF+F FYRQA  TIFL PL  +F R  
Sbjct: 3   MESKKPYLMVTIIQVIYTIMFLISKAVFNGGMNTFVFVFYRQAFATIFLAPLAFFFERKS 62

Query: 61  MASLSKGDLLKIFMQAFLGFCRFTFGFNAYGLGVKYTSATLGAAAFNCIPVTTFFFAFIL 120
              LS    +KIFM +  G    T   +  G+ + YTSATL AA    +P  TFF A + 
Sbjct: 63  APPLSFVTFIKIFMLSLFGV---TLSLDLNGIALSYTSATLAAATTASLPAITFFLALLF 122

Query: 121 RVEKVNTKKASGMAKVGGIMLCITGVAVITFYKGPYLKPLNNVHLFQTQQ-------AHV 180
            +E++  K   G AK+ GI +C+ GV ++  YKGP LK     H +  Q+        HV
Sbjct: 123 GMERLKVKSIQGTAKLVGITVCMGGVIILAIYKGPLLKLPLCPHFYHGQEHPHRNNPGHV 182

Query: 181 SS-KKEWILGCSLLLLSSLAVGLWFVLQVWVLRTCPSPLVVTFGQTFSSSIQSFVVAIAI 240
           S     W+ GC L++ S++  GLW VLQ  VL+  PS L  T      SSIQSFV+AIA+
Sbjct: 183 SGGSTSWLKGCVLMITSNILWGLWLVLQGRVLKVYPSKLYFTTLHCLLSSIQSFVIAIAL 242

Query: 241 ERNPSQWKLAWNISLAAILYCGVFVVSIGNYLSSWVVKKKGPVFQAVTTPFNLIVT---- 300
           ER+ S WKL WN+ L A++YCG  V  +  YL SWV++K+GPVF ++ TP +L+ T    
Sbjct: 243 ERDISAWKLGWNLRLVAVIYCGFIVTGVAYYLQSWVIEKRGPVFLSMFTPLSLLFTLLSS 302

Query: 301 -----------------LIVLSLYSVLWGKKKE 305
                            L+++ LY VLWGK +E
Sbjct: 303 AILLCEIISLGSIVGGLLLIIGLYCVLWGKSRE 332

[Close]

BLAST of Cucsa.101200 vs. TAIR10
Match: AT1G43650.1 (AT1G43650.1 nodulin MtN21 /EamA-like transporter family protein)

HSP 1 Score: 200.3 bits (508), Expect = 1.8e-51
Identity = 119/325 (36.62%), Postives = 168/325 (51.69%), Query Frame = 1


Query: 1   MNSKKPYFAALLCQTIFAGMSLLSKASYSSGMNTFIFFFYRQAVGTIFLLPLTIYFSRNE 60
           M   K   A +  Q ++AGM LLSK + S G N F+F FYRQA   + L P   +   ++
Sbjct: 2   MMEHKANMAMVFVQIVYAGMPLLSKVAISQGTNPFVFVFYRQAFAALALSPFAFFLESSK 61

Query: 61  MASLSKGDLLKIFMQAFLGFCRFTFGFNAYGLGVKYTSATLGAAAFNCIPVTTFFFAFIL 120
            + LS   LLKIF   F+  C  T   N Y + ++ T+AT  AA  N IP  TF  A + 
Sbjct: 62  SSPLSFILLLKIF---FISLCGLTLSLNLYYVAIENTTATFAAATTNAIPSITFVLALLF 121

Query: 121 RVEKVNTKKASGMAKVGGIMLCITGVAVITFYKGPYLKPLNNVHLFQTQQAHVSSKKEWI 180
           R+E V  KK+ G+AKV G M+ + G  V  F KGP L  +N+ +        V S K  +
Sbjct: 122 RLETVTLKKSHGVAKVTGSMVGMLGALVFAFVKGPSL--INHYNSSTIPNGTVPSTKNSV 181

Query: 181 LGCSLLLLSSLAVGLWFVLQVWVLRTCPSPLVVTFGQTFSSSIQSFVVAIAIERNPSQWK 240
            G   +L ++    LW ++Q  V++  P+ L +   Q   S IQS V A+A+ RNPS WK
Sbjct: 182 KGSITMLAANTCWCLWIIMQSKVMKEYPAKLRLVALQCLFSCIQSAVWAVAVNRNPSVWK 241

Query: 241 LAWNISLAAILYCGVFVVSIGNYLSSWVVKKKGPVFQAVTTPFNLIVT------------ 300
           + + + L ++ YCG+ V  +  +L  W ++KKGPVF A+ TP  LI+T            
Sbjct: 242 IEFGLPLLSMAYCGIMVTGLTYWLQVWAIEKKGPVFTALYTPLALILTCIVSSFLFKETF 301

Query: 301 ---------LIVLSLYSVLWGKKKE 305
                    L+V  LY  LWGK KE
Sbjct: 302 YLGSVGGAVLLVCGLYLGLWGKTKE 321

[Close]

BLAST of Cucsa.101200 vs. TAIR10
Match: AT5G07050.1 (AT5G07050.1 nodulin MtN21 /EamA-like transporter family protein)

HSP 1 Score: 191.8 bits (486), Expect = 6.5e-49
Identity = 116/357 (32.49%), Postives = 182/357 (50.98%), Query Frame = 1


Query: 1   MNSKKPYFAALLCQTIFAGMSLLSKASYSSGMNTFIFFFYRQAVGTIFLLPLTIYFSRNE 60
           + S KPYFA +  Q  +AGM++++K S ++GM+ ++   YR A+ T  + P   +F R  
Sbjct: 13  LTSSKPYFAMISLQFGYAGMNIITKISLNTGMSHYVLVVYRHAIATAVIAPFAFFFERKA 72

Query: 61  MASLSKGDLLKIFMQAF-LGFCRFTFGFNAYGLGVKYTSATLGAAAFNCIPVTTFFFAFI 120
              ++      IFMQ F LG        N Y +G+KYTS T   A  N +P  TF  A +
Sbjct: 73  QPKIT----FSIFMQLFILGLLGPVIDQNFYYMGLKYTSPTFSCAMSNMLPAMTFILAVL 132

Query: 121 LRVEKVNTKKASGMAKVGGIMLCITGVAVITFYKGP--------YLKPLNNVHLFQTQQA 180
            R+E ++ KK    AK+ G ++ + G  ++T YKGP        Y+   ++ H   T   
Sbjct: 133 FRMEMLDLKKLWCQAKIAGTVVTVAGAMLMTIYKGPIVELFWTKYMHIQDSSHANTTSSK 192

Query: 181 HVSSKKEWILGCSLLLLSSLAVGLWFVLQVWVLRT-CPSPLVVTFGQTFSSSIQSFVVAI 240
           + SS KE++ G  LL+ ++LA    FVLQ  +L+T     L +T    F  ++Q+  V  
Sbjct: 193 NSSSDKEFLKGSILLIFATLAWASLFVLQAKILKTYAKHQLSLTTLICFIGTLQAVAVTF 252

Query: 241 AIERNPSQWKLAWNISLAAILYCGVFVVSIGNYLSSWVVKKKGPVFQAVTTPFNLIV--- 300
            +E NPS W++ W+++L A  Y G+   SI  Y+   V+KK+GPVF    +P  +++   
Sbjct: 253 VMEHNPSAWRIGWDMNLLAAAYSGIVASSISYYVQGIVMKKRGPVFATAFSPLMMVIVAV 312

Query: 301 ------------------TLIVLSLYSVLWGKKKE---ACCHDA--SNNNSITNSVQ 322
                              LIV+ LY+VLWGK+KE     C  A   +N+ +T  V+
Sbjct: 313 MGSFVLAEKIFLGGVIGAVLIVIGLYAVLWGKQKENQVTICELAKIDSNSKVTEDVE 365

[Close]

BLAST of Cucsa.101200 vs. TAIR10
Match: AT2G39510.1 (AT2G39510.1 nodulin MtN21 /EamA-like transporter family protein)

HSP 1 Score: 169.1 bits (427), Expect = 4.5e-42
Identity = 105/323 (32.51%), Postives = 159/323 (49.23%), Query Frame = 1


Query: 5   KPYFAALLCQTIFAGMSLLSKASYSSGMNTFIFFFYRQAVGTIFLLPLTIYFSRNEMASL 64
           KP+   +  Q  +AG+S+++K + + GM+  +   YR  V TIF+ P   +  R     +
Sbjct: 7   KPFITVVSLQFGYAGLSIIAKFALNQGMSPHVLASYRHIVATIFIAPFAYFLDRKIRPKM 66

Query: 65  SKGDLLKIFMQAFLGFCRFTFGFNAYGLGVKYTSATLGAAAFNCIPVTTFFFAFILRVEK 124
           +     KI +   LG    T   N Y  G+KYTSAT  AA  N +P   F  A+I R+EK
Sbjct: 67  TLSIFFKILL---LGLLEPTIDQNLYYTGMKYTSATFTAAMTNVLPAFAFIMAWIFRLEK 126

Query: 125 VNTKKASGMAKVGGIMLCITGVAVITFYKGPYLK-PLNNVHLFQTQQAHVSSKKEWILGC 184
           VN KK    AK+ G ++ + G  ++T  KGP +  P  N H      ++   K++   G 
Sbjct: 127 VNVKKIHSQAKILGTIVTVGGAMLMTVVKGPLIPLPWANPHDIHQDSSNTGVKQDLTKGA 186

Query: 185 SLLLLSSLAVGLWFVLQVWVLRTCPSPLVVTFGQTFSSSIQSFVVAIAIER-NPSQWKLA 244
           SL+ +  +    +  LQ   L++ P  L +T    F  SI+S +VA+ IER NPS W + 
Sbjct: 187 SLIAIGCICWAGFINLQAITLKSYPVELSLTAYICFLGSIESTIVALFIERGNPSAWAIH 246

Query: 245 WNISLAAILYCGVFVVSIGNYLSSWVVKKKGPVFQAVTTPFNLIVT-------------- 304
            +  L A +Y GV    IG Y+   ++K +GPVF     P ++++               
Sbjct: 247 LDSKLLAAVYGGVICSGIGYYVQGVIMKTRGPVFVTAFNPLSMVIVAILGSIILAEVMFL 306

[Close]

BLAST of Cucsa.101200 vs. TAIR10
Match: AT4G30420.1 (AT4G30420.1 nodulin MtN21 /EamA-like transporter family protein)

HSP 1 Score: 166.8 bits (421), Expect = 2.2e-41
Identity = 95/335 (28.36%), Postives = 162/335 (48.36%), Query Frame = 1


Query: 9   AALLCQTIFAGMSLLSKASYSSGMNTFIFFFYRQAVGTIFLLPLTIYFSRNEMASLSKGD 68
           A  + Q  +AG++L ++A+   G++  +F  YRQA  TIF+ P      R    ++S  D
Sbjct: 2   AMTMIQLCYAGVTLFARATLVHGLSPRVFILYRQAFATIFIFPFLYLSRRKSKIAISSLD 61

Query: 69  LLKIFMQAFLGFCRFTFGFNAYGLGVKYTSATLGAAAFNCIPVTTFFFAFILRVEKVNTK 128
           L    +   +     T   N Y  G+  TS+++G+A  N IP  TF  +F+   EK+N +
Sbjct: 62  LKSFSLIFLVSLIGITINQNLYLEGLYLTSSSMGSAVGNIIPAITFLISFLAGYEKLNLR 121

Query: 129 KASGMAKVGGIMLCITGVAVITFYKGP-YLKPLNNVHLFQTQQAHVSSKKEWILGCSLLL 188
              G+AK+ G +LC+ G   +T  +GP  L   + + + ++   H+  +  W++GC  L 
Sbjct: 122 DIRGLAKIAGTILCVAGAISMTLLRGPKILNSESALPIAKSVLGHLKDQNTWLIGCLFLF 181

Query: 189 LSSLAVGLWFVLQVWVLRTCPSPLVVTFGQTFSSSIQSFVVAIAIERNPSQWKLAWNISL 248
            S+L    W +LQV +    P  L ++       +IQ  VV   +E++P+ W L      
Sbjct: 182 SSTLCWSFWLILQVPISAYYPDNLSLSAWMCLFGTIQCAVVTFFLEKDPNAWILHSYSEF 241

Query: 249 AAILYCGVFVVSIGNYLSSWVVKKKGPVFQAVTTPF-NLIVTL----------------- 308
           A  LY G+   ++   + +W + K+GPVF A+  P   +IVT+                 
Sbjct: 242 ATCLYAGIGASALSFTVQAWAIAKRGPVFSALFNPLCTVIVTILAALFFHEEIYTGSLIG 301

Query: 309 ---IVLSLYSVLWGKKKEACCHDASNNNSITNSVQ 322
              ++L LY+VLWGK K+   +    +N   + V+
Sbjct: 302 GLGVILGLYTVLWGKAKDVMMNQDQRDNDQKSEVK 336

[Close]

BLAST of Cucsa.101200 vs. NCBI nr
Match: gi|778717857|ref|XP_004148667.2| (PREDICTED: WAT1-related protein At5g64700 isoform X1 [Cucumis sativus])

HSP 1 Score: 617.8 bits (1592), Expect = 1.0e-173
Identity = 318/342 (92.98%), Postives = 318/342 (92.98%), Query Frame = 1


Query: 1   MNSKKPYFAALLCQTIFAGMSLLSKASYSSGMNTFIFFFYRQAVGTIFLLPLTIYFSRNE 60
           MNSKKPYFAALLCQTIFAGMSLLSKASYSSGMNTFIFFFYRQAVGTIFLLPLTIYFSRNE
Sbjct: 5   MNSKKPYFAALLCQTIFAGMSLLSKASYSSGMNTFIFFFYRQAVGTIFLLPLTIYFSRNE 64

Query: 61  MASLSKGDLLKIFMQAFLGFCRFTFGFNAYGLGVKYTSATLGAAAFNCIPVTTFFFAFIL 120
           MASLSKGDLLKIFMQAFLG   FTFGFNAYGLGVKYTSATLGAAAFNCIPVTTFFFAFIL
Sbjct: 65  MASLSKGDLLKIFMQAFLG---FTFGFNAYGLGVKYTSATLGAAAFNCIPVTTFFFAFIL 124

Query: 121 RVEKVNTKKASGMAKVGGIMLCITGVAVITFYKGPYLKPLNNVHLFQTQQAHVSSKKEWI 180
           RVEKVNTKKASGMAKVGGIMLCITGVAVITFYKGPYLKPLNNVHLFQTQQAHVSSKKEWI
Sbjct: 125 RVEKVNTKKASGMAKVGGIMLCITGVAVITFYKGPYLKPLNNVHLFQTQQAHVSSKKEWI 184

Query: 181 LGCSLLLLSSLAVGLWFVLQVWVLRTCPSPLVVTFGQTFSSSIQSFVVAIAIERNPSQWK 240
           LGCSLLLLSSLAVGLWFVLQVWVLRTCPSPLVVTFGQTFSSSIQSFVVAIAIERNPSQWK
Sbjct: 185 LGCSLLLLSSLAVGLWFVLQVWVLRTCPSPLVVTFGQTFSSSIQSFVVAIAIERNPSQWK 244

Query: 241 LAWNISLAAILYCGVFVVSIGNYLSSWVVKKKGPVFQAVTTPFNLIVTLI---------- 300
           LAWNISLAAILYCGVFVVSIGNYLSSWVVKKKGPVFQAVTTPFNLIVTLI          
Sbjct: 245 LAWNISLAAILYCGVFVVSIGNYLSSWVVKKKGPVFQAVTTPFNLIVTLIGSEFLFKYGI 304

Query: 301 -----------VLSLYSVLWGKKKEACCHDASNNNSITNSVQ 322
                      VLSLYSVLWGKKKEACCHDASNNNSITNSVQ
Sbjct: 305 CLGSGIGAILLVLSLYSVLWGKKKEACCHDASNNNSITNSVQ 343

[Close]

BLAST of Cucsa.101200 vs. NCBI nr
Match: gi|659081076|ref|XP_008441137.1| (PREDICTED: WAT1-related protein At5g64700-like [Cucumis melo])

HSP 1 Score: 503.8 bits (1296), Expect = 2.2e-139
Identity = 259/338 (76.63%), Postives = 282/338 (83.43%), Query Frame = 1


Query: 1   MNSKKPYFAALLCQTIFAGMSLLSKASYSSGMNTFIFFFYRQAVGTIFLLPLTIYFSRNE 60
           MNS KP FAAL+CQ  FAGM+LLSKA++S GMNTFIFFFYRQAVGTIFLLPLTIYFSR +
Sbjct: 5   MNSIKPCFAALVCQITFAGMNLLSKAAFSFGMNTFIFFFYRQAVGTIFLLPLTIYFSRKD 64

Query: 61  MASLSKGDLLKIFMQAFLGFCRFTFGFN-AYGLGVKYTSATLGAAAFNCIPVTTFFFAFI 120
           MASLSKGD+LK FMQA LGF   TFG N AYGLG+KYTSAT+GAAA+NC+PVTTFFFA +
Sbjct: 65  MASLSKGDMLKNFMQAILGF---TFGLNIAYGLGIKYTSATMGAAAYNCVPVTTFFFALV 124

Query: 121 LRVEKVNTKKASGMAKVGGIMLCITGVAVITFYKGPYLKPLNNVHLFQT--QQAHVSSKK 180
            R+EKVN KKASG+AKVGG +LCI GVAV+ FYKGP+   L   HLFQT  QQ+HVSSKK
Sbjct: 125 SRMEKVNLKKASGIAKVGGSILCIAGVAVLAFYKGPHFNTLFKFHLFQTDHQQSHVSSKK 184

Query: 181 EWILGCSLLLLSSLAVGLWFVLQVWVLRTCPSPLVVTFGQTFSSSIQSFVVAIAIERNPS 240
           EWILGC LL L+ L+ GLWFVLQ WVLR+CPSPLVVTFGQTF S+IQSF VAIAIERNPS
Sbjct: 185 EWILGCFLLFLTCLSWGLWFVLQAWVLRSCPSPLVVTFGQTFLSAIQSFFVAIAIERNPS 244

Query: 241 QWKLAWNISLAAILYCGVFVVSIGNYLSSWVVKKKGPVFQAVTTPFNLIVTLI------- 300
           QWKLAWNISLAAILYCGVFVVSIGNYLSSWVVKKKGPVFQAVTTPFNLI+TLI       
Sbjct: 245 QWKLAWNISLAAILYCGVFVVSIGNYLSSWVVKKKGPVFQAVTTPFNLIITLIGSEFLLK 304

Query: 301 --------------VLSLYSVLWGKKKEACCHDASNNN 315
                         VLSLYS+LWGKKKEA CHDASNNN
Sbjct: 305 DGISLGSTIGALLLVLSLYSILWGKKKEASCHDASNNN 339

[Close]

BLAST of Cucsa.101200 vs. NCBI nr
Match: gi|778717861|ref|XP_011657770.1| (PREDICTED: WAT1-related protein At5g64700 isoform X2 [Cucumis sativus])

HSP 1 Score: 500.0 bits (1286), Expect = 3.1e-138
Identity = 258/282 (91.49%), Postives = 258/282 (91.49%), Query Frame = 1


Query: 61  MASLSKGDLLKIFMQAFLGFCRFTFGFNAYGLGVKYTSATLGAAAFNCIPVTTFFFAFIL 120
           MASLSKGDLLKIFMQAFLG   FTFGFNAYGLGVKYTSATLGAAAFNCIPVTTFFFAFIL
Sbjct: 1   MASLSKGDLLKIFMQAFLG---FTFGFNAYGLGVKYTSATLGAAAFNCIPVTTFFFAFIL 60

Query: 121 RVEKVNTKKASGMAKVGGIMLCITGVAVITFYKGPYLKPLNNVHLFQTQQAHVSSKKEWI 180
           RVEKVNTKKASGMAKVGGIMLCITGVAVITFYKGPYLKPLNNVHLFQTQQAHVSSKKEWI
Sbjct: 61  RVEKVNTKKASGMAKVGGIMLCITGVAVITFYKGPYLKPLNNVHLFQTQQAHVSSKKEWI 120

Query: 181 LGCSLLLLSSLAVGLWFVLQVWVLRTCPSPLVVTFGQTFSSSIQSFVVAIAIERNPSQWK 240
           LGCSLLLLSSLAVGLWFVLQVWVLRTCPSPLVVTFGQTFSSSIQSFVVAIAIERNPSQWK
Sbjct: 121 LGCSLLLLSSLAVGLWFVLQVWVLRTCPSPLVVTFGQTFSSSIQSFVVAIAIERNPSQWK 180

Query: 241 LAWNISLAAILYCGVFVVSIGNYLSSWVVKKKGPVFQAVTTPFNLIVTLI---------- 300
           LAWNISLAAILYCGVFVVSIGNYLSSWVVKKKGPVFQAVTTPFNLIVTLI          
Sbjct: 181 LAWNISLAAILYCGVFVVSIGNYLSSWVVKKKGPVFQAVTTPFNLIVTLIGSEFLFKYGI 240

Query: 301 -----------VLSLYSVLWGKKKEACCHDASNNNSITNSVQ 322
                      VLSLYSVLWGKKKEACCHDASNNNSITNSVQ
Sbjct: 241 CLGSGIGAILLVLSLYSVLWGKKKEACCHDASNNNSITNSVQ 279

[Close]

BLAST of Cucsa.101200 vs. NCBI nr
Match: gi|659080837|ref|XP_008441006.1| (PREDICTED: WAT1-related protein At5g64700-like isoform X1 [Cucumis melo])

HSP 1 Score: 500.0 bits (1286), Expect = 3.1e-138
Identity = 252/339 (74.34%), Postives = 278/339 (82.01%), Query Frame = 1


Query: 3   SKKPYFAALLCQTIFAGMSLLSKASYSSGMNTFIFFFYRQAVGTIFLLPLTIYFSRNEMA 62
           SKKPYF AL+CQ  FAGMSL SKA+++SGMNT+IF FYRQA+GTI LLPLTIYF+R EM 
Sbjct: 7   SKKPYFGALVCQITFAGMSLFSKAAFASGMNTYIFLFYRQALGTIVLLPLTIYFARKEMG 66

Query: 63  SLSKGDLLKIFMQAFLGFCRFTFGFNAYGLGVKYTSATLGAAAFNCIPVTTFFFAFILRV 122
           SLSKGD++KIF+QA LGF   T G N YGLGVKYTSATLGAAAFNC+PVTTFFFA I R+
Sbjct: 67  SLSKGDVMKIFVQAILGF---TVGLNVYGLGVKYTSATLGAAAFNCVPVTTFFFALISRM 126

Query: 123 EKVNTKKASGMAKVGGIMLCITGVAVITFYKGPYLKPLNNVHLFQTQQAHVSSKKEWILG 182
           EKVN +KASGMAK+GG++LCI GVAV+ FYKGPYLKPL   HLFQTQQ+HVSSKKEWILG
Sbjct: 127 EKVNIRKASGMAKIGGLILCIAGVAVLAFYKGPYLKPLFKFHLFQTQQSHVSSKKEWILG 186

Query: 183 CSLLLLSSLAVGLWFVLQVWVLRTCPSPLVVTFGQTFSSSIQSFVVAIAIERNPSQWKLA 242
           C LL L+ L  GLW+VLQ WVLR+CPSPLV+TFGQTFSS+IQSF VAIAIERNPS+WKLA
Sbjct: 187 CFLLFLTCLTWGLWYVLQAWVLRSCPSPLVLTFGQTFSSAIQSFFVAIAIERNPSEWKLA 246

Query: 243 WNISLAAILYCGVFVVSIGNYLSSWVVKKKGPVFQAVTTPFNLIVTLI------------ 302
           WNISLAAILYCG FV+ +GNYLSSWVVKKKGPVFQAVTTPFNLI TLI            
Sbjct: 247 WNISLAAILYCGAFVIPVGNYLSSWVVKKKGPVFQAVTTPFNLIFTLIGSEFLLNDGIGL 306

Query: 303 ---------VLSLYSVLWGKKKEACCHDASNNNSITNSV 321
                    VLSLYSVLWGKKKEA C D  NN   TNSV
Sbjct: 307 GSIIGAILLVLSLYSVLWGKKKEASCLDTKNN---TNSV 339

[Close]

BLAST of Cucsa.101200 vs. NCBI nr
Match: gi|449461821|ref|XP_004148640.1| (PREDICTED: WAT1-related protein At5g64700 [Cucumis sativus])

HSP 1 Score: 498.8 bits (1283), Expect = 7.0e-138
Identity = 248/337 (73.59%), Postives = 275/337 (81.60%), Query Frame = 1


Query: 1   MNSKKPYFAALLCQTIFAGMSLLSKASYSSGMNTFIFFFYRQAVGTIFLLPLTIYFSRNE 60
           M+SKKPYF AL+CQ  FAGMSL SKA++ SGMNT+IF FYRQA G+I LLPLTIYF+R E
Sbjct: 10  MDSKKPYFGALVCQLTFAGMSLFSKAAFVSGMNTYIFLFYRQAFGSIVLLPLTIYFARKE 69

Query: 61  MASLSKGDLLKIFMQAFLGFCRFTFGFNAYGLGVKYTSATLGAAAFNCIPVTTFFFAFIL 120
           M SLSK D++KIF+Q  LGF   T G N YGLGVKYTSATLGAAAFNC+PVTTFFFA + 
Sbjct: 70  MRSLSKADVMKIFLQGLLGF---TIGLNVYGLGVKYTSATLGAAAFNCVPVTTFFFALLS 129

Query: 121 RVEKVNTKKASGMAKVGGIMLCITGVAVITFYKGPYLKPLNNVHLFQTQQAHVSSKKEWI 180
           R+EKVN +KASGMAKVGGI+LCI GVAV+ FYKGPYLKPL N HLFQTQQ+HVSSKKEWI
Sbjct: 130 RMEKVNIRKASGMAKVGGIILCIAGVAVLAFYKGPYLKPLFNFHLFQTQQSHVSSKKEWI 189

Query: 181 LGCSLLLLSSLAVGLWFVLQVWVLRTCPSPLVVTFGQTFSSSIQSFVVAIAIERNPSQWK 240
           LGC LL L+ L  GLW+VLQ WVLR+CPSPLV+TFGQTFSS+IQSFVVAIAIERNPS+WK
Sbjct: 190 LGCFLLFLTCLTWGLWYVLQAWVLRSCPSPLVLTFGQTFSSAIQSFVVAIAIERNPSEWK 249

Query: 241 LAWNISLAAILYCGVFVVSIGNYLSSWVVKKKGPVFQAVTTPFNLIVTLI---------- 300
           L WNISLAAILYCG FV+ +GNYLSSWVVKKKGPVFQAVTTPFNLI TLI          
Sbjct: 250 LGWNISLAAILYCGTFVIPVGNYLSSWVVKKKGPVFQAVTTPFNLIFTLIGSEFLLNDGI 309

Query: 301 -----------VLSLYSVLWGKKKEACCHDASNNNSI 317
                      VLSLYSVLWGKKKEA C D  NN ++
Sbjct: 310 SLGSIIGAILLVLSLYSVLWGKKKEASCFDTENNTNV 343

The following BLAST results are available for this feature:

BLAST of Cucsa.101200 vs. Swiss-Prot


Match Name	E-value	Identity	Description
WTR45_ARATH	3.8e-67	41.74	WAT1-related protein At5g64700 OS=Arabidopsis thaliana GN=At5g64700 PE=2 SV=1	[more]
WTR7_ARATH	3.2e-50	36.62	WAT1-related protein At1g43650 OS=Arabidopsis thaliana GN=At1g43650 PE=2 SV=1	[more]
WTR38_ARATH	1.1e-47	32.49	WAT1-related protein At5g07050 OS=Arabidopsis thaliana GN=At5g07050 PE=2 SV=1	[more]
WTR14_ARATH	8.0e-41	32.51	WAT1-related protein At2g39510 OS=Arabidopsis thaliana GN=At2g39510 PE=2 SV=1	[more]
WTR37_ARATH	3.9e-40	28.36	WAT1-related protein At4g30420 OS=Arabidopsis thaliana GN=At4g30420 PE=3 SV=1	[more]

BLAST of Cucsa.101200 vs. TrEMBL


Match Name	E-value	Identity	Description
A0A0A0KF97_CUCSA	7.2e-174	92.98	WAT1-related protein OS=Cucumis sativus GN=Csa_6G485120 PE=3 SV=1	[more]
A0A0A0KHU9_CUCSA	4.1e-129	73.58	WAT1-related protein OS=Cucumis sativus GN=Csa_6G485110 PE=3 SV=1	[more]
A0A0A0KFR8_CUCSA	8.1e-101	56.29	WAT1-related protein OS=Cucumis sativus GN=Csa_6G484610 PE=3 SV=1	[more]
A0A0A0KJ33_CUCSA	6.7e-87	49.24	WAT1-related protein OS=Cucumis sativus GN=Csa_6G485670 PE=3 SV=1	[more]
A0A0A0KFA3_CUCSA	9.7e-86	50.15	WAT1-related protein OS=Cucumis sativus GN=Csa_6G485160 PE=3 SV=1	[more]

BLAST of Cucsa.101200 vs. TAIR10


Match Name	E-value	Identity	Description
AT5G64700.1	2.1e-68	41.74	nodulin MtN21 /EamA-like transporter family protein	[more]
AT1G43650.1	1.8e-51	36.62	nodulin MtN21 /EamA-like transporter family protein	[more]
AT5G07050.1	6.5e-49	32.49	nodulin MtN21 /EamA-like transporter family protein	[more]
AT2G39510.1	4.5e-42	32.51	nodulin MtN21 /EamA-like transporter family protein	[more]
AT4G30420.1	2.2e-41	28.36	nodulin MtN21 /EamA-like transporter family protein	[more]

BLAST of Cucsa.101200 vs. NCBI nr


Match Name	E-value	Identity	Description
gi\|778717857\|ref\|XP_004148667.2\|	1.0e-173	92.98	PREDICTED: WAT1-related protein At5g64700 isoform X1 [Cucumis sativus]	[more]
gi\|659081076\|ref\|XP_008441137.1\|	2.2e-139	76.63	PREDICTED: WAT1-related protein At5g64700-like [Cucumis melo]	[more]
gi\|778717861\|ref\|XP_011657770.1\|	3.1e-138	91.49	PREDICTED: WAT1-related protein At5g64700 isoform X2 [Cucumis sativus]	[more]
gi\|659080837\|ref\|XP_008441006.1\|	3.1e-138	74.34	PREDICTED: WAT1-related protein At5g64700-like isoform X1 [Cucumis melo]	[more]
gi\|449461821\|ref\|XP_004148640.1\|	7.0e-138	73.59	PREDICTED: WAT1-related protein At5g64700 [Cucumis sativus]	[more]

Annotated Terms

The following terms have been associated with this gene:

Vocabulary: **INTERPRO**
Term	Definition
IPR000620	EamA_dom

Vocabulary: **Cellular Component**
Term	Definition
GO:0016021	integral component of membrane
GO:0016020	membrane

Vocabulary: **Molecular Function**
Term	Definition
GO:0022857	transmembrane transporter activity

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0055085	transmembrane transport
cellular_component	GO:0016021	integral component of membrane
cellular_component	GO:0016020	membrane
molecular_function	GO:0022857	transmembrane transporter activity

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Cucsa.101200.1	Cucsa.101200.1	mRNA

InterPro

Analysis Name: InterPro Annotations of cucumber (Gy14)
Date Performed: 2017-01-17


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR000620	EamA domain	PFAM	PF00892	EamA	coord: 5..150 score: 1.2
None	No IPR available	PANTHER	PTHR31218:SF15	SUBFAMILY NOT NAMED	coord: 1..304 score: 3.1E
None	No IPR available	unknown	SSF103481	Multidrug resistance efflux transporter EmrE	coord: 40..153 score: 1.0

Synteny

The following gene(s) are orthologous to this gene:


Gene	Orthologue	Organism	Block
Cucsa.101200	Cla008457	Watermelon (97103) v1	cgywmB137

The following gene(s) are paralogous to this gene:

None

The following block(s) are covering this gene:


Gene	Organism	Block
Cucsa.101200	Wax gourd	cgywgoB150
Cucsa.101200	Wax gourd	cgywgoB153
Cucsa.101200	Cucumber (Gy14) v1	cgycgyB046
Cucsa.101200	Cucurbita maxima (Rimu)	cgycmaB0229
Cucsa.101200	Cucurbita maxima (Rimu)	cgycmaB0231
Cucsa.101200	Cucurbita maxima (Rimu)	cgycmaB0235
Cucsa.101200	Cucurbita moschata (Rifu)	cgycmoB0225
Cucsa.101200	Cucurbita moschata (Rifu)	cgycmoB0227
Cucsa.101200	Cucurbita moschata (Rifu)	cgycmoB0233
Cucsa.101200	Wild cucumber (PI 183967)	cgycpiB117
Cucsa.101200	Wild cucumber (PI 183967)	cgycpiB122
Cucsa.101200	Cucumber (Chinese Long) v2	cgycuB115
Cucsa.101200	Cucumber (Chinese Long) v2	cgycuB121
Cucsa.101200	Melon (DHL92) v3.5.1	cgymeB132
Cucsa.101200	Watermelon (Charleston Gray)	cgywcgB134
Cucsa.101200	Watermelon (Charleston Gray)	cgywcgB135
Cucsa.101200	Watermelon (97103) v1	cgywmB140
Cucsa.101200	Cucurbita pepo (Zucchini)	cgycpeB0211
Cucsa.101200	Cucurbita pepo (Zucchini)	cgycpeB0220
Cucsa.101200	Cucurbita pepo (Zucchini)	cgycpeB0224
Cucsa.101200	Bottle gourd (USVL1VR-Ls)	cgylsiB125
Cucsa.101200	Melon (DHL92) v3.6.1	cgymedB132
Cucsa.101200	Silver-seed gourd	carcgyB0280
Cucsa.101200	Silver-seed gourd	carcgyB0918
Cucsa.101200	Cucumber (Chinese Long) v3	cgycucB126
Cucsa.101200	Watermelon (97103) v2	cgywmbB129
Cucsa.101200	Watermelon (97103) v2	cgywmbB127