Cucsa.095190 (gene) Cucumber (Gy14) v1

Overview
NameCucsa.095190
Typegene
OrganismCucumis sativus (Cucumber (Gy14) v1)
DescriptionEnoyl CoA hydratase-like protein
Locationscaffold00919 : 1114841 .. 1116242 (+)
Genome Viewer
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
CACAAGTTCACTTCTCAAAGTATACACTTTCCAATTCTCCAAAATTATAACCCACCAATTTCCGCCGAAGGCCAATGGAGGACTACAAATGCATAAAAATCGAGCGACAAAACCCAAACTCCGGAGTCTTCCATCTCCGCCTCCATCGTCCATCACACTACAACGCTCTCACAACCCAACTCTTCACCGAACTTCGTCAAGCCTTCTCACATCTAGACCAAAACCCCGATGTCCATGTCATCATCCTATCTGGATCCGGCAAACACTTCTGCGCCGGAATCGACCTCAAATCCACAGCCTCTAACTTCGAGAAGCATCTATCGGAGGAACGGGGACGAGCCGGGGAAAGAATCCGACGGGAAATCAAGTGGATGCAAGAGTCAATCACGGCGATTGAGGAGTGCCGGAAGCCGGTGATAGCAAGCATCCATGGAGGGTGTGTTGGAGGGGGAATTGATATAGTGACGGCATGTGATTTGAGGTATTGCACGGCGGAGGCGGTGTTTTCGGTGAGGGAGGTTAAATTGGCGATTACGGCGGATCTTGGGACTTTACAGAGGCTGCCGAGAATCGTAGGGTATGGGAAGGCGGCGGAGTTGGCGTTGACGGGCCGGAATTTTTCGGGCTTGGAGGCTAAGGAATTGGGCTTGGTTTCTCGAACTTTTGATTCTATATCGAAGCTTCAAGATGGAGTCCTCAAAATCGCTGAAGGTAATGAAATTCTCTATACTAACCAATTTTCCTATGCCTTCTCTTTTAGTACCACTCTCATTTCTTACTAGTTTAATATCTTCTGATTAATCTAGTTAAATTAGTGGTTAAAGCATATGATAGATTGTTGTGTAATGTGTATGAGATTGGGAAAAaGGAATGAAAAGTGGATGGTGAATGCAGAAATAGCATCATCATCTCCATTGGCTGTGGTGGGAACAAAGGCAGTGCTACTAAAAAGCAGAGACCTAAATGTAGAACAAGGGTTAGATTATGTTGCAACTTGGAACTCAGGGACTCTACTCTCCGATGATCTCAAAGAAGCCATCTCCGCTCAAGCTAACAAACGCAAACCCAAGTTTTCCAAGCTTTAAGATGGAGAGAGACGTGAACACTTTATCTCTTCATCAAGAAACTCAAGTTACGCCTCTTGCTCTCAAAGCAGCTACATCCTACATTTTATTCTTGATTTCTTGTTCTCAATAATGACATGAAATACTATCGTGTATGTGCCTTTTCTTTTCTCAATCACCTTTGGTTTTtCTTCTTCTTAATAAAAACCAAAAaGTAAAATTGAATATTTCAAGATCCATACTATGTACATTCATTACATAGGACGATAAACGATGCAAGATCACATCGATTGTATTTATCATTCATTACATGGGACGATAAACGATGCAAGATCACA

mRNA sequence

CACAAGTTCACTTCTCAAAGTATACACTTTCCAATTCTCCAAAATTATAACCCACCAATTTCCGCCGAAGGCCAATGGAGGACTACAAATGCATAAAAATCGAGCGACAAAACCCAAACTCCGGAGTCTTCCATCTCCGCCTCCATCGTCCATCACACTACAACGCTCTCACAACCCAACTCTTCACCGAACTTCGTCAAGCCTTCTCACATCTAGACCAAAACCCCGATGTCCATGTCATCATCCTATCTGGATCCGGCAAACACTTCTGCGCCGGAATCGACCTCAAATCCACAGCCTCTAACTTCGAGAAGCATCTATCGGAGGAACGGGGACGAGCCGGGGAAAGAATCCGACGGGAAATCAAGTGGATGCAAGAGTCAATCACGGCGATTGAGGAGTGCCGGAAGCCGGTGATAGCAAGCATCCATGGAGGGTGTGTTGGAGGGGGAATTGATATAGTGACGGCATGTGATTTGAGGTATTGCACGGCGGAGGCGGTGTTTTCGGTGAGGGAGGTTAAATTGGCGATTACGGCGGATCTTGGGACTTTACAGAGGCTGCCGAGAATCGTAGGGTATGGGAAGGCGGCGGAGTTGGCGTTGACGGGCCGGAATTTTTCGGGCTTGGAGGCTAAGGAATTGGGCTTGGTTTCTCGAACTTTTGATTCTATATCGAAGCTTCAAGATGGAGTCCTCAAAATCGCTGAAGAAATAGCATCATCATCTCCATTGGCTGTGGTGGGAACAAAGGCAGTGCTACTAAAAAGCAGAGACCTAAATGTAGAACAAGGGTTAGATTATGTTGCAACTTGGAACTCAGGGACTCTACTCTCCGATGATCTCAAAGAAGCCATCTCCGCTCAAGCTAACAAACGCAAACCCAAGTTTTCCAAGCTTTAAGATGGAGAGAGACGTGAACACTTTATCTCTTCATCAAGAAACTCAAGTTACGCCTCTTGCTCTCAAAGCAGCTACATCCTACATTTTATTCTTGATTTCTTGTTCTCAATAATGACATGAAATACTATCGTGTATGTGCCTTTTCTTTTCTCAATCACCTTTGGTTTTTCTTCTTCTTAATAAAAACCAAaaagtaaaattgaatatttcaagatccatactatgtacattcattacataggacgataaacgatgcaagatcacatcgattgtattTATCATTCATTACATGGGACGATAAACGATGCAAGATCACA

Coding sequence (CDS)

ATGGAGGACTACAAATGCATAAAAATCGAGCGACAAAACCCAAACTCCGGAGTCTTCCATCTCCGCCTCCATCGTCCATCACACTACAACGCTCTCACAACCCAACTCTTCACCGAACTTCGTCAAGCCTTCTCACATCTAGACCAAAACCCCGATGTCCATGTCATCATCCTATCTGGATCCGGCAAACACTTCTGCGCCGGAATCGACCTCAAATCCACAGCCTCTAACTTCGAGAAGCATCTATCGGAGGAACGGGGACGAGCCGGGGAAAGAATCCGACGGGAAATCAAGTGGATGCAAGAGTCAATCACGGCGATTGAGGAGTGCCGGAAGCCGGTGATAGCAAGCATCCATGGAGGGTGTGTTGGAGGGGGAATTGATATAGTGACGGCATGTGATTTGAGGTATTGCACGGCGGAGGCGGTGTTTTCGGTGAGGGAGGTTAAATTGGCGATTACGGCGGATCTTGGGACTTTACAGAGGCTGCCGAGAATCGTAGGGTATGGGAAGGCGGCGGAGTTGGCGTTGACGGGCCGGAATTTTTCGGGCTTGGAGGCTAAGGAATTGGGCTTGGTTTCTCGAACTTTTGATTCTATATCGAAGCTTCAAGATGGAGTCCTCAAAATCGCTGAAGAAATAGCATCATCATCTCCATTGGCTGTGGTGGGAACAAAGGCAGTGCTACTAAAAAGCAGAGACCTAAATGTAGAACAAGGGTTAGATTATGTTGCAACTTGGAACTCAGGGACTCTACTCTCCGATGATCTCAAAGAAGCCATCTCCGCTCAAGCTAACAAACGCAAACCCAAGTTTTCCAAGCTTTAA

Protein sequence

MEDYKCIKIERQNPNSGVFHLRLHRPSHYNALTTQLFTELRQAFSHLDQNPDVHVIILSGSGKHFCAGIDLKSTASNFEKHLSEERGRAGERIRREIKWMQESITAIEECRKPVIASIHGGCVGGGIDIVTACDLRYCTAEAVFSVREVKLAITADLGTLQRLPRIVGYGKAAELALTGRNFSGLEAKELGLVSRTFDSISKLQDGVLKIAEEIASSSPLAVVGTKAVLLKSRDLNVEQGLDYVATWNSGTLLSDDLKEAISAQANKRKPKFSKL*
Homology
[Close]
BLAST of Cucsa.095190 vs. Swiss-Prot
Match: DCI1_ARATH (Delta(3,5)-Delta(2,4)-dienoyl-CoA isomerase, peroxisomal OS=Arabidopsis thaliana GN=DCI1 PE=2 SV=1)

HSP 1 Score: 357.5 bits (916), Expect = 1.4e-97
Identity = 178/276 (64.49%), Postives = 220/276 (79.71%), Query Frame = 1 

Query: 1   MEDYKCIKIERQNPNSGVFHLRLHRPSHYNALTTQLFTELRQAFSHLDQNPDVHVIILSG 60
           ME YK ++I R+N +S VFHL ++RPSH NAL+   F E  +A S LDQNPDV VIILSG
Sbjct: 3   MESYKTLEIIRKNTDSSVFHLIINRPSHLNALSLDFFIEFPKALSSLDQNPDVSVIILSG 62

Query: 61  SGKHFCAGIDLKSTAS-NFEKHLSEERGRAGERIRREIKWMQESITAIEECRKPVIASIH 120
           +GKHFC+GIDL S +S + +     +RGR+ E++RR+IK MQ +ITAIE+CRKPVIA+IH
Sbjct: 63  AGKHFCSGIDLNSLSSISTQSSSGNDRGRSSEQLRRKIKSMQAAITAIEQCRKPVIAAIH 122

Query: 121 GGCVGGGIDIVTACDLRYCTAEAVFSVREVKLAITADLGTLQRLPRIVGYGKAAELALTG 180
           G C+GGG+D++TACD+RYC+ +A FS++EV LAI ADLGTLQRLP IVGY  A ELALT 
Sbjct: 123 GACIGGGVDLITACDIRYCSEDAFFSIKEVDLAIVADLGTLQRLPSIVGYANAMELALTA 182

Query: 181 RNFSGLEAKELGLVSRTFDSISKLQDGVLKIAEEIASSSPLAVVGTKAVLLKSRDLNVEQ 240
           R FSG EAK+LGLVS+ F S S+L +GV  IAE I   SPLAV GTKAVLL+SR+++VEQ
Sbjct: 183 RRFSGSEAKDLGLVSKVFGSKSELDNGVTTIAEGIGGKSPLAVTGTKAVLLRSREVSVEQ 242

Query: 241 GLDYVATWNSGTLLSDDLKEAISAQANKRKPKFSKL 276
           GLDYVATWNS  L+SDDL EA+SAQ  KRKP+F+KL
Sbjct: 243 GLDYVATWNSAMLISDDLNEAVSAQMMKRKPRFAKL 278

[Close]
BLAST of Cucsa.095190 vs. Swiss-Prot
Match: ECH1_MOUSE (Delta(3,5)-Delta(2,4)-dienoyl-CoA isomerase, mitochondrial OS=Mus musculus GN=Ech1 PE=1 SV=1)

HSP 1 Score: 215.3 bits (547), Expect = 8.3e-55
Identity = 110/279 (39.43%), Postives = 169/279 (60.57%), Query Frame = 1 

Query: 1   MEDYKCIKIERQNPNSGVFHLRLHRPSHYNALTTQLFTELRQAFSHLDQNPDVHVIILSG 60
           + D+    I+  +    V H++L+RP   NA+    + EL + F  + ++ D   +++SG
Sbjct: 49  VSDHNYESIQVTSAQKHVLHVQLNRPEKRNAMNRAFWRELVECFQKISKDSDCRAVVVSG 108

Query: 61  SGKHFCAGIDLKSTASNFEKHLSEERGRAGERIRREIKWMQESITAIEECRKPVIASIHG 120
           +GK F +GIDL   AS   +   ++  R    +R  I   Q++ T IE+C KPVIA+IHG
Sbjct: 109 AGKMFTSGIDLMDMASELMQPSGDDAARIAWYLRDLISKYQKTFTVIEKCPKPVIAAIHG 168

Query: 121 GCVGGGIDIVTACDLRYCTAEAVFSVREVKLAITADLGTLQRLPRIVG-YGKAAELALTG 180
           GC+GGG+D+V+ACD+RYCT +A F ++EV + + AD+GTLQRLP+++G      EL  + 
Sbjct: 169 GCIGGGVDLVSACDIRYCTQDAFFQIKEVDMGLAADVGTLQRLPKVIGNQSLVNELTFSA 228

Query: 181 RNFSGLEAKELGLVSRTFDSISKLQDGVLKIAEEIASSSPLAVVGTKAVLLKSRDLNVEQ 240
           R     EA + GLVSR F     + +    +A +I+S SP+AV G+K  L+ SRD +V++
Sbjct: 229 RKMMADEALDSGLVSRVFQDKDAMLNAAFALAADISSKSPVAVQGSKINLIYSRDHSVDE 288

Query: 241 GLDYVATWNSGTLLSDDLKEAISAQANKRKPK---FSKL 276
            LDY+ATWN   L + D+ +++ A   KR  K   FSKL
Sbjct: 289 SLDYMATWNMSMLQTQDIIKSVQAAMEKRDTKSITFSKL 327

[Close]
BLAST of Cucsa.095190 vs. Swiss-Prot
Match: ECH1_RAT (Delta(3,5)-Delta(2,4)-dienoyl-CoA isomerase, mitochondrial OS=Rattus norvegicus GN=Ech1 PE=1 SV=2)

HSP 1 Score: 213.4 bits (542), Expect = 3.2e-54
Identity = 110/279 (39.43%), Postives = 170/279 (60.93%), Query Frame = 1 

Query: 1   MEDYKCIKIERQNPNSGVFHLRLHRPSHYNALTTQLFTELRQAFSHLDQNPDVHVIILSG 60
           + D+    I+  +    V H++L+RP   NA+    + EL + F  + ++ D   +++SG
Sbjct: 49  VSDHNYESIQVTSAQKHVLHVQLNRPEKRNAMNRAFWRELVECFQKISKDSDCRAVVVSG 108

Query: 61  SGKHFCAGIDLKSTASNFEKHLSEERGRAGERIRREIKWMQESITAIEECRKPVIASIHG 120
           +GK F +GIDL   AS+  +   ++  R    +R  I   Q++ T IE+C KPVIA+IHG
Sbjct: 109 AGKMFTSGIDLMDMASDILQPPGDDVARIAWYLRDLISRYQKTFTVIEKCPKPVIAAIHG 168

Query: 121 GCVGGGIDIVTACDLRYCTAEAVFSVREVKLAITADLGTLQRLPRIVGYGKAA-ELALTG 180
           GC+GGG+D+++ACD+RYCT +A F V+EV + + AD+GTLQRLP+++G      EL  T 
Sbjct: 169 GCIGGGVDLISACDIRYCTQDAFFQVKEVDVGLAADVGTLQRLPKVIGNRSLVNELTFTA 228

Query: 181 RNFSGLEAKELGLVSRTFDSISKLQDGVLKIAEEIASSSPLAVVGTKAVLLKSRDLNVEQ 240
           R     EA + GLVSR F     + +    +A +I+S SP+AV G+K  L+ SRD +V++
Sbjct: 229 RKMMADEALDSGLVSRVFPDKDVMLNAAFALAADISSKSPVAVQGSKINLIYSRDHSVDE 288

Query: 241 GLDYVATWNSGTLLSDDLKEAISAQANKRKPK---FSKL 276
            LDY+ATWN   L + D+ +++ A   K+  K   FSKL
Sbjct: 289 SLDYMATWNMSMLQTQDIIKSVQAAMEKKDSKSITFSKL 327

[Close]
BLAST of Cucsa.095190 vs. Swiss-Prot
Match: ECH1_PONAB (Delta(3,5)-Delta(2,4)-dienoyl-CoA isomerase, mitochondrial OS=Pongo abelii GN=ECH1 PE=2 SV=1)

HSP 1 Score: 213.4 bits (542), Expect = 3.2e-54
Identity = 111/262 (42.37%), Postives = 163/262 (62.21%), Query Frame = 1 

Query: 18  VFHLRLHRPSHYNALTTQLFTELRQAFSHLDQNPDVHVIILSGSGKHFCAGIDLKSTASN 77
           V H++L+RP+  NA+    + E+ + F+ + ++ D   +++SG+GK F AG+DL   AS+
Sbjct: 67  VLHVQLNRPNKRNAMNKVFWREMVECFNKISRDADCRAVVISGAGKMFTAGVDLMDMASD 126

Query: 78  FEKHLSEERGRAGERIRREIKWMQESITAIEECRKPVIASIHGGCVGGGIDIVTACDLRY 137
             +   ++  R    +R  I   QE+   IE+C KPVIA++HGGC+GGG+D+VTACD+RY
Sbjct: 127 ILQPKGDDVARISWYLRDIITRYQETFNVIEKCPKPVIAAVHGGCIGGGVDLVTACDIRY 186

Query: 138 CTAEAVFSVREVKLAITADLGTLQRLPRIVG-YGKAAELALTGRNFSGLEAKELGLVSRT 197
           C  +A F V+EV + + AD+GTLQRLP+++G      ELA T R     EA   GLVSR 
Sbjct: 187 CAQDAFFQVKEVDVGLAADVGTLQRLPKVIGNQSLVNELAFTARKMMADEALGSGLVSRV 246

Query: 198 FDSISKLQDGVLKIAEEIASSSPLAVVGTKAVLLKSRDLNVEQGLDYVATWNSGTLLSDD 257
           F     + D  L +A EI+S SP+AV  TK  LL SRD +V + L+YVA+WN   L + D
Sbjct: 247 FPDKEVMLDAALALAAEISSKSPVAVQSTKVNLLYSRDHSVAESLNYVASWNMSMLQTQD 306

Query: 258 LKEAISAQANKRKPK---FSKL 276
           L +++ A    ++ K   FSKL
Sbjct: 307 LMKSVQAATENKELKSVTFSKL 328

[Close]
BLAST of Cucsa.095190 vs. Swiss-Prot
Match: ECH1_HUMAN (Delta(3,5)-Delta(2,4)-dienoyl-CoA isomerase, mitochondrial OS=Homo sapiens GN=ECH1 PE=1 SV=2)

HSP 1 Score: 213.0 bits (541), Expect = 4.1e-54
Identity = 112/262 (42.75%), Postives = 162/262 (61.83%), Query Frame = 1 

Query: 18  VFHLRLHRPSHYNALTTQLFTELRQAFSHLDQNPDVHVIILSGSGKHFCAGIDLKSTASN 77
           V H++L+RP+  NA+    + E+ + F+ + ++ D   +++SG+GK F AGIDL   AS+
Sbjct: 67  VLHVQLNRPNKRNAMNKVFWREMVECFNKISRDADCRAVVISGAGKMFTAGIDLMDMASD 126

Query: 78  FEKHLSEERGRAGERIRREIKWMQESITAIEECRKPVIASIHGGCVGGGIDIVTACDLRY 137
             +   ++  R    +R  I   QE+   IE C KPVIA++HGGC+GGG+D+VTACD+RY
Sbjct: 127 ILQPKGDDVARISWYLRDIITRYQETFNVIERCPKPVIAAVHGGCIGGGVDLVTACDIRY 186

Query: 138 CTAEAVFSVREVKLAITADLGTLQRLPRIVG-YGKAAELALTGRNFSGLEAKELGLVSRT 197
           C  +A F V+EV + + AD+GTLQRLP+++G      ELA T R     EA   GLVSR 
Sbjct: 187 CAQDAFFQVKEVDVGLAADVGTLQRLPKVIGNQSLVNELAFTARKMMADEALGSGLVSRV 246

Query: 198 FDSISKLQDGVLKIAEEIASSSPLAVVGTKAVLLKSRDLNVEQGLDYVATWNSGTLLSDD 257
           F     + D  L +A EI+S SP+AV  TK  LL SRD +V + L+YVA+WN   L + D
Sbjct: 247 FPDKEVMLDAALALAAEISSKSPVAVQSTKVNLLYSRDHSVAESLNYVASWNMSMLQTQD 306

Query: 258 LKEAISAQANKRKPK---FSKL 276
           L +++ A    ++ K   FSKL
Sbjct: 307 LVKSVQATTENKELKTVTFSKL 328

[Close]
BLAST of Cucsa.095190 vs. TrEMBL
Match: A0A0A0KXB8_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_4G011790 PE=3 SV=1)

HSP 1 Score: 541.2 bits (1393), Expect = 7.4e-151
Identity = 274/275 (99.64%), Postives = 275/275 (100.00%), Query Frame = 1 

Query: 1   MEDYKCIKIERQNPNSGVFHLRLHRPSHYNALTTQLFTELRQAFSHLDQNPDVHVIILSG 60
           MEDYKCIKIERQNPNSGVFHLRLHRPSHYNALTTQLFTELRQAFSHLDQNPDVHVIILSG
Sbjct: 1   MEDYKCIKIERQNPNSGVFHLRLHRPSHYNALTTQLFTELRQAFSHLDQNPDVHVIILSG 60

Query: 61  SGKHFCAGIDLKSTASNFEKHLSEERGRAGERIRREIKWMQESITAIEECRKPVIASIHG 120
           SGKHFCAGIDLKSTASNFEKHLSEERGRAGERIRREIKWMQESITAIEECRKPVIASIHG
Sbjct: 61  SGKHFCAGIDLKSTASNFEKHLSEERGRAGERIRREIKWMQESITAIEECRKPVIASIHG 120

Query: 121 GCVGGGIDIVTACDLRYCTAEAVFSVREVKLAITADLGTLQRLPRIVGYGKAAELALTGR 180
           GCVGGGIDIVTACDLRYCTAEAVFSVREVKLAITADLGTLQRLPRIVGYGKAAELALTGR
Sbjct: 121 GCVGGGIDIVTACDLRYCTAEAVFSVREVKLAITADLGTLQRLPRIVGYGKAAELALTGR 180

Query: 181 NFSGLEAKELGLVSRTFDSISKLQDGVLKIAEEIASSSPLAVVGTKAVLLKSRDLNVEQG 240
           NFSGLEAKELGLVSRTFDSISKLQDGVLKIAEEIASSSPLAVVGTKAVLLKSRDLNVEQG
Sbjct: 181 NFSGLEAKELGLVSRTFDSISKLQDGVLKIAEEIASSSPLAVVGTKAVLLKSRDLNVEQG 240

Query: 241 LDYVATWNSGTLLSDDLKEAISAQANKRKPKFSKL 276
           LDYVATWNSGTLLSDDLKEAISAQA+KRKPKFSKL
Sbjct: 241 LDYVATWNSGTLLSDDLKEAISAQASKRKPKFSKL 275

[Close]
BLAST of Cucsa.095190 vs. TrEMBL
Match: A0A0V0I419_SOLCH (Putative delta(3,5)-Delta(2,4)-dienoyl-CoA isomerase, mitochondrial-like OS=Solanum chacoense PE=3 SV=1)

HSP 1 Score: 370.5 bits (950), Expect = 1.7e-99
Identity = 184/275 (66.91%), Postives = 229/275 (83.27%), Query Frame = 1 

Query: 1   MEDYKCIKIERQNPNSGVFHLRLHRPSHYNALTTQLFTELRQAFSHLDQNPDVHVIILSG 60
           ME+YK +KI +++PNS V++L L+RP+H NAL+   FTE  +A S LDQNP+V VIIL+G
Sbjct: 1   MEEYKSLKIIQKSPNSLVYYLYLNRPTHLNALSRDFFTEFPKAISSLDQNPNVAVIILAG 60

Query: 61  SGKHFCAGIDLKSTASNFEKHLSEERGRAGERIRREIKWMQESITAIEECRKPVIASIHG 120
           SGKHFC+GIDL++    F++  S + GR  E +RR IK++QE+ITA+E CRKPVIA++HG
Sbjct: 61  SGKHFCSGIDLQTLGDIFKESDSVDCGRKVETLRRHIKFLQEAITALECCRKPVIAAVHG 120

Query: 121 GCVGGGIDIVTACDLRYCTAEAVFSVREVKLAITADLGTLQRLPRIVGYGKAAELALTGR 180
            C+GG IDI+TACD+RYC+++A FSV+EV LAITADLGTLQRLP IVG+G A ELALTGR
Sbjct: 121 ACIGGAIDIITACDIRYCSSDAFFSVKEVDLAITADLGTLQRLPSIVGFGNAMELALTGR 180

Query: 181 NFSGLEAKELGLVSRTFDSISKLQDGVLKIAEEIASSSPLAVVGTKAVLLKSRDLNVEQG 240
            F+G EAK+LGLVS+ F S   L++GV  +AEEIA+ SPLAV+GTKAVLL+SRDL VEQG
Sbjct: 181 RFTGSEAKDLGLVSKLFTSKEALEEGVKVVAEEIATKSPLAVIGTKAVLLRSRDLTVEQG 240

Query: 241 LDYVATWNSGTLLSDDLKEAISAQANKRKPKFSKL 276
           LDYVATWNSGTLLSDDLKEAISA + KRKPKF+KL
Sbjct: 241 LDYVATWNSGTLLSDDLKEAISAHSQKRKPKFAKL 275

[Close]
BLAST of Cucsa.095190 vs. TrEMBL
Match: M5Y9B9_PRUPE (Uncharacterized protein OS=Prunus persica GN=PRUPE_ppa009837mg PE=3 SV=1)

HSP 1 Score: 369.8 bits (948), Expect = 2.9e-99
Identity = 187/275 (68.00%), Postives = 221/275 (80.36%), Query Frame = 1 

Query: 1   MEDYKCIKIERQNPNSGVFHLRLHRPSHYNALTTQLFTELRQAFSHLDQNPDVHVIILSG 60
           M +Y  ++I ++NPNS VF+L L+RPSH NAL+   FTE  +A S LDQN DV+VI+LSG
Sbjct: 1   MANYTTLEIVQENPNSAVFYLYLNRPSHRNALSRDFFTEFPRALSSLDQNSDVNVIVLSG 60

Query: 61  SGKHFCAGIDLKSTASNFEKHLSEERGRAGERIRREIKWMQESITAIEECRKPVIASIHG 120
           +G HFCAGIDLK+  S  ++ +S +RGRAGE+ RREIK +QE+ITAIE CRKPV+ASIHG
Sbjct: 61  AGNHFCAGIDLKTLTSISDQFVSGDRGRAGEKFRREIKELQEAITAIERCRKPVVASIHG 120

Query: 121 GCVGGGIDIVTACDLRYCTAEAVFSVREVKLAITADLGTLQRLPRIVGYGKAAELALTGR 180
            C+GGG+DIVTACD+RYC+ +A FSV+EV LAITADLGTLQRLP IVGYG A ELALTGR
Sbjct: 121 ACIGGGVDIVTACDIRYCSKDAFFSVKEVDLAITADLGTLQRLPSIVGYGNALELALTGR 180

Query: 181 NFSGLEAKELGLVSRTFDSISKLQDGVLKIAEEIASSSPLAVVGTKAVLLKSRDLNVEQG 240
            FS  EAKELGLVSR F S  +L +GV  +AE I   SPLAV GTKAVL +SR+LNVEQG
Sbjct: 181 TFSSSEAKELGLVSRVFGSREELDNGVRVVAEGIGLKSPLAVRGTKAVLQRSRELNVEQG 240

Query: 241 LDYVATWNSGTLLSDDLKEAISAQANKRKPKFSKL 276
           LDYVATWNS  LLSDDL EA+SA A KRKP F+KL
Sbjct: 241 LDYVATWNSAMLLSDDLSEAVSAYAQKRKPIFAKL 275

[Close]
BLAST of Cucsa.095190 vs. TrEMBL
Match: A0A078H7T1_BRANA (BnaA09g16350D protein OS=Brassica napus GN=BnaA09g16350D PE=3 SV=1)

HSP 1 Score: 360.9 bits (925), Expect = 1.4e-96
Identity = 182/276 (65.94%), Postives = 222/276 (80.43%), Query Frame = 1 

Query: 1   MEDYKCIKIERQNPNSGVFHLRLHRPSHYNALTTQLFTELRQAFSHLDQNPDVHVIILSG 60
           ME YK ++I R+N +S VFHL L+RPS  NAL+   F EL +A S LDQNPDV VIILSG
Sbjct: 1   MESYKTLEIVRKNTDSSVFHLILNRPSQLNALSLDFFDELPEALSSLDQNPDVSVIILSG 60

Query: 61  SGKHFCAGIDLKSTAS-NFEKHLSEERGRAGERIRREIKWMQESITAIEECRKPVIASIH 120
           +GKHFC+GIDL S +S + +     +RGR+ E +RR IK MQ +ITA+EECRKPVIA++H
Sbjct: 61  AGKHFCSGIDLASLSSISAQASSGGDRGRSSEGLRRRIKSMQAAITAVEECRKPVIAAVH 120

Query: 121 GGCVGGGIDIVTACDLRYCTAEAVFSVREVKLAITADLGTLQRLPRIVGYGKAAELALTG 180
           G C+GGG+D+VTACD+RYC+ EA FS++EV LAI ADLGTLQRLPRIVG+ KA ELALT 
Sbjct: 121 GACIGGGVDLVTACDVRYCSEEAFFSIKEVDLAIVADLGTLQRLPRIVGHAKAMELALTA 180

Query: 181 RNFSGLEAKELGLVSRTFDSISKLQDGVLKIAEEIASSSPLAVVGTKAVLLKSRDLNVEQ 240
           R FSG EAK+LGLVS+ F S S+L  GV  IAE IA+ SPLAV GTKAVLL+SR+++VEQ
Sbjct: 181 RRFSGSEAKDLGLVSQVFGSKSELDKGVTMIAERIAAKSPLAVTGTKAVLLRSREMSVEQ 240

Query: 241 GLDYVATWNSGTLLSDDLKEAISAQANKRKPKFSKL 276
           GLDYVATWNSG L+SDDL E++SAQ  KR+P+FSKL
Sbjct: 241 GLDYVATWNSGMLISDDLNESVSAQMMKRQPRFSKL 276

[Close]
BLAST of Cucsa.095190 vs. TrEMBL
Match: W9QLC1_9ROSA (Delta(3,5)-Delta(2,4)-dienoyl-CoA isomerase OS=Morus notabilis GN=L484_017491 PE=3 SV=1)

HSP 1 Score: 360.9 bits (925), Expect = 1.4e-96
Identity = 179/275 (65.09%), Postives = 218/275 (79.27%), Query Frame = 1 

Query: 1   MEDYKCIKIERQNPNSGVFHLRLHRPSHYNALTTQLFTELRQAFSHLDQNPDVHVIILSG 60
           ME +K ++I ++NPNS VFHL L+RPSH NAL+   F+E  +A + LDQNP  +V+IL G
Sbjct: 1   MEKFKTLEIVQKNPNSSVFHLHLNRPSHRNALSPDFFSEFPRALASLDQNPSANVVILYG 60

Query: 61  SGKHFCAGIDLKSTASNFEKHLSEERGRAGERIRREIKWMQESITAIEECRKPVIASIHG 120
           +G HFC+GIDLKS  S  E+ LS +RGR GE +RR IK +Q+++TAIE CRKPVIA+IHG
Sbjct: 61  AGDHFCSGIDLKSLNSIAERALSGDRGRGGEWLRRHIKGLQDAVTAIERCRKPVIAAIHG 120

Query: 121 GCVGGGIDIVTACDLRYCTAEAVFSVREVKLAITADLGTLQRLPRIVGYGKAAELALTGR 180
            C+GGG+DIVTACD+R+CT +A FSV+EV LAITADLGTLQRLP IVG+G A ELALTGR
Sbjct: 121 ACIGGGVDIVTACDIRFCTKDAFFSVKEVDLAITADLGTLQRLPSIVGFGNAMELALTGR 180

Query: 181 NFSGLEAKELGLVSRTFDSISKLQDGVLKIAEEIASSSPLAVVGTKAVLLKSRDLNVEQG 240
             SG EAK++GLVS  F S  ++ D V  +AE IAS SPLAV GTKAVLL+SRD+N+EQG
Sbjct: 181 RLSGQEAKQMGLVSGVFGSKQEMDDAVGFVAEGIASKSPLAVSGTKAVLLRSRDMNLEQG 240

Query: 241 LDYVATWNSGTLLSDDLKEAISAQANKRKPKFSKL 276
           LDYVATWNS  LLSDDL E +SAQ  KRKP F+KL
Sbjct: 241 LDYVATWNSSMLLSDDLNETVSAQIQKRKPTFAKL 275

[Close]
BLAST of Cucsa.095190 vs. TAIR10
Match: AT5G43280.1 (AT5G43280.1 delta(3,5),delta(2,4)-dienoyl-CoA isomerase 1)

HSP 1 Score: 357.5 bits (916), Expect = 7.6e-99
Identity = 178/276 (64.49%), Postives = 220/276 (79.71%), Query Frame = 1 

Query: 1   MEDYKCIKIERQNPNSGVFHLRLHRPSHYNALTTQLFTELRQAFSHLDQNPDVHVIILSG 60
           ME YK ++I R+N +S VFHL ++RPSH NAL+   F E  +A S LDQNPDV VIILSG
Sbjct: 3   MESYKTLEIIRKNTDSSVFHLIINRPSHLNALSLDFFIEFPKALSSLDQNPDVSVIILSG 62

Query: 61  SGKHFCAGIDLKSTAS-NFEKHLSEERGRAGERIRREIKWMQESITAIEECRKPVIASIH 120
           +GKHFC+GIDL S +S + +     +RGR+ E++RR+IK MQ +ITAIE+CRKPVIA+IH
Sbjct: 63  AGKHFCSGIDLNSLSSISTQSSSGNDRGRSSEQLRRKIKSMQAAITAIEQCRKPVIAAIH 122

Query: 121 GGCVGGGIDIVTACDLRYCTAEAVFSVREVKLAITADLGTLQRLPRIVGYGKAAELALTG 180
           G C+GGG+D++TACD+RYC+ +A FS++EV LAI ADLGTLQRLP IVGY  A ELALT 
Sbjct: 123 GACIGGGVDLITACDIRYCSEDAFFSIKEVDLAIVADLGTLQRLPSIVGYANAMELALTA 182

Query: 181 RNFSGLEAKELGLVSRTFDSISKLQDGVLKIAEEIASSSPLAVVGTKAVLLKSRDLNVEQ 240
           R FSG EAK+LGLVS+ F S S+L +GV  IAE I   SPLAV GTKAVLL+SR+++VEQ
Sbjct: 183 RRFSGSEAKDLGLVSKVFGSKSELDNGVTTIAEGIGGKSPLAVTGTKAVLLRSREVSVEQ 242

Query: 241 GLDYVATWNSGTLLSDDLKEAISAQANKRKPKFSKL 276
           GLDYVATWNS  L+SDDL EA+SAQ  KRKP+F+KL
Sbjct: 243 GLDYVATWNSAMLISDDLNEAVSAQMMKRKPRFAKL 278

[Close]
BLAST of Cucsa.095190 vs. TAIR10
Match: AT4G16800.1 (AT4G16800.1 ATP-dependent caseinolytic (Clp) protease/crotonase family protein)

HSP 1 Score: 125.2 bits (313), Expect = 6.4e-29
Identity = 81/269 (30.11%), Postives = 130/269 (48.33%), Query Frame = 1 

Query: 7   IKIER-QNPNSGVFHLRLHRPSHYNALTTQLFTELRQAFSHLDQNPDVHVIILSGSGKH- 66
           +K+ R    +SG+  + L RP   NA+  ++   L+ AF  + Q+    V+++       
Sbjct: 43  VKLNRLSGSDSGIIEVNLDRPVTKNAINKEMLKSLQNAFESIHQDNSARVVMIRSLVPGV 102

Query: 67  FCAGIDLKSTASNFEKHLSEERGRAGERIRREIKWMQESITAIEECRKPVIASIHGGCVG 126
           FCAG DLK           E R  +   +   +  ++   + IE    P IA+I G  +G
Sbjct: 103 FCAGADLK-----------ERRTMSPSEVHTYVNSLRYMFSFIEALSIPTIAAIEGAALG 162

Query: 127 GGIDIVTACDLRYCTAEAVFSVREVKLAITADLGTLQRLPRIVGYGKAAELALTGRNFSG 186
           GG+++  ACDLR C   AVF + E  LAI    G  QRL R+VG   + EL  TGR    
Sbjct: 163 GGLEMALACDLRICGENAVFGLPETGLAIIPGAGGTQRLSRLVGRSVSKELIFTGRKIDA 222

Query: 187 LEAKELGLVSRTFDSISKLQDGVLKIAEEIASSSPLAVVGTKAVLLKSRDLNVEQGLDYV 246
           +EA   GLV+    +  +  +  +++A++I    PLA+   K  + +  + N+  GL+  
Sbjct: 223 IEAANKGLVNICV-TAGEAHEKAIEMAQQINEKGPLAIKMAKKAIDEGIETNMASGLEVE 282

Query: 247 ATWNSGTLLSDDLKEAISAQANKRKPKFS 274
                  L + D  E ++A A KRKP ++
Sbjct: 283 EMCYQKLLNTQDRLEGLAAFAEKRKPLYT 299

[Close]
BLAST of Cucsa.095190 vs. TAIR10
Match: AT4G16210.1 (AT4G16210.1 enoyl-CoA hydratase/isomerase A)

HSP 1 Score: 102.8 bits (255), Expect = 3.4e-22
Identity = 63/233 (27.04%), Postives = 113/233 (48.50%), Query Frame = 1 

Query: 9   IERQNPNSGVFHLRLHRPSHYNALTTQLFTELRQAFSHLDQNPDVHVIILSGSGKHFCAG 68
           I+ +  + G+  + ++RP   N+LT  +  +L +AF  +D +  V V+I +GSG+ FC+G
Sbjct: 10  IQVKKESGGIAVITINRPKSLNSLTRAMMVDLAKAFKDMDSDESVQVVIFTGSGRSFCSG 69

Query: 69  IDLKSTASNFEKHLSEERGRAGERIRREIKWMQESITAIEECRKPVIASIHGGCVGGGID 128
           +DL +  S F+  + +                 + +  +E  RKP+I +I+G  +  G +
Sbjct: 70  VDLTAAESVFKGDVKDPE--------------TDPVVQMERLRKPIIGAINGFAITAGFE 129

Query: 129 IVTACDLRYCTAEAVFSVREVKLAITADLGTLQRLPRIVGYGKAAELALTGRNFSGLEAK 188
           +  ACD+   +  A F     +  I    G  Q+L RI+G  KA E++LT    +   A 
Sbjct: 130 LALACDILVASRGAKFMDTHARFGIFPSWGLSQKLSRIIGANKAREVSLTSMPLTADVAG 189

Query: 189 ELGLVSRTFDSISKLQDGVLKIAEEIASSSPLAVVGTKAVLLKSRDLNVEQGL 242
           +LG V+   +    L+    +IAE I  +    V+  K+V+     L++   L
Sbjct: 190 KLGFVNHVVEEGEALKK-AREIAEAIIKNEQGMVLRIKSVINDGLKLDLGHAL 227

[Close]
BLAST of Cucsa.095190 vs. TAIR10
Match: AT4G29010.1 (AT4G29010.1 Enoyl-CoA hydratase/isomerase family)

HSP 1 Score: 89.4 bits (220), Expect = 3.9e-18
Identity = 58/211 (27.49%), Postives = 102/211 (48.34%), Query Frame = 1 

Query: 5   KCIKIERQNPNSGVFHLRLHRPSHYNALTTQLFTELRQAFSHLDQNPDVHVIILSGSGKH 64
           K I +  +  N GV  + +  P   N+L + + + L++ F   +Q  DV  I+L G+   
Sbjct: 3   KKIGVTMEVGNDGVAVITISNPP-VNSLASPIISGLKEKFRDANQRNDVKAIVLIGNNGR 62

Query: 65  FCAGIDLKSTASNFEKHLSEERGRAGERIRREIKWMQESITAIEECRKPVIASIHGGCVG 124
           F  G D+         ++ ++  + G+        ++     +E+ RKPV+A++ G  +G
Sbjct: 63  FSGGFDI---------NVFQQVHKTGDLSLMPEVSVELVCNLMEDSRKPVVAAVEGLALG 122

Query: 125 GGIDIVTACDLRYCTAEAVFSVREVKLAITADLGTLQRLPRIVGYGKAAELALTGRNFSG 184
           GG+++  AC  R    +A   + E+ L +    G  QRLPR+VG  KA ++ L  ++ S 
Sbjct: 123 GGLELAMACHARVAAPKAQLGLPELTLGVIPGFGGTQRLPRLVGLAKATDMILLSKSISS 182

Query: 185 LEAKELGLVSRTF---DSISKLQDGVLKIAE 213
            E  +LGL+       D +S  +   L IAE
Sbjct: 183 EEGHKLGLIDALVPPGDVLSTSRKWALDIAE 203

[Close]
BLAST of Cucsa.095190 vs. TAIR10
Match: AT1G60550.1 (AT1G60550.1 enoyl-CoA hydratase/isomerase D)

HSP 1 Score: 83.2 bits (204), Expect = 2.8e-16
Identity = 80/266 (30.08%), Postives = 123/266 (46.24%), Query Frame = 1 

Query: 15  NSGVFHLRLHRPSHYNALTTQLFTELRQAFSHLDQNPDVHVIILSGSG-KHFCAGIDLKS 74
           + G+  + ++RP   NA   Q   EL +AF+    +  V VIIL+G G K FC+G D   
Sbjct: 84  DEGIAKITINRPERRNAFRPQTVKELMRAFNDARDDSSVGVIILTGKGTKAFCSGGD--- 143

Query: 75  TASNFEKHLSEERGRAGERIRREIKWMQESITAIEECRKPVIASIHGGCVGGGIDIVTAC 134
                 + L  + G A       +  +   +  I    KPVIA + G  VGGG  +   C
Sbjct: 144 ------QALRTQDGYADPNDVGRLNVLDLQV-QIRRLPKPVIAMVAGYAVGGGHILHMVC 203

Query: 135 DLRYCTAEAVFSVREVKL-AITADLGTLQRLPRIVGYGKAAELALTGRNFSGLEAKELGL 194
           DL      A+F     K+ +  A  G+   + R+VG  KA E+    R ++  EA+++GL
Sbjct: 204 DLTIAADNAIFGQTGPKVGSFDAGYGS-SIMSRLVGPKKAREMWFMTRFYTASEAEKMGL 263

Query: 195 VSRTFDSISKLQDGVLKIAEEIASSSPLAVVGTKAVLLKSRDLNVE-QGLDYVATWNSGT 254
           ++ T   +  L+   +K   EI  +SP A+   KA L    D +   QGL         T
Sbjct: 264 IN-TVVPLEDLEKETVKWCREILRNSPTAIRVLKAALNAVDDGHAGLQGLG-----GDAT 323

Query: 255 LL---SDDLKEAISAQANKRKPKFSK 275
           LL   +++  E  +A  ++R P FSK
Sbjct: 324 LLFYGTEEATEGRTAYMHRRPPDFSK 332

[Close]
BLAST of Cucsa.095190 vs. NCBI nr
Match: gi|449468826|ref|XP_004152122.1| (PREDICTED: delta(3,5)-Delta(2,4)-dienoyl-CoA isomerase, mitochondrial [Cucumis sativus])

HSP 1 Score: 541.2 bits (1393), Expect = 1.1e-150
Identity = 274/275 (99.64%), Postives = 275/275 (100.00%), Query Frame = 1 

Query: 1   MEDYKCIKIERQNPNSGVFHLRLHRPSHYNALTTQLFTELRQAFSHLDQNPDVHVIILSG 60
           MEDYKCIKIERQNPNSGVFHLRLHRPSHYNALTTQLFTELRQAFSHLDQNPDVHVIILSG
Sbjct: 1   MEDYKCIKIERQNPNSGVFHLRLHRPSHYNALTTQLFTELRQAFSHLDQNPDVHVIILSG 60

Query: 61  SGKHFCAGIDLKSTASNFEKHLSEERGRAGERIRREIKWMQESITAIEECRKPVIASIHG 120
           SGKHFCAGIDLKSTASNFEKHLSEERGRAGERIRREIKWMQESITAIEECRKPVIASIHG
Sbjct: 61  SGKHFCAGIDLKSTASNFEKHLSEERGRAGERIRREIKWMQESITAIEECRKPVIASIHG 120

Query: 121 GCVGGGIDIVTACDLRYCTAEAVFSVREVKLAITADLGTLQRLPRIVGYGKAAELALTGR 180
           GCVGGGIDIVTACDLRYCTAEAVFSVREVKLAITADLGTLQRLPRIVGYGKAAELALTGR
Sbjct: 121 GCVGGGIDIVTACDLRYCTAEAVFSVREVKLAITADLGTLQRLPRIVGYGKAAELALTGR 180

Query: 181 NFSGLEAKELGLVSRTFDSISKLQDGVLKIAEEIASSSPLAVVGTKAVLLKSRDLNVEQG 240
           NFSGLEAKELGLVSRTFDSISKLQDGVLKIAEEIASSSPLAVVGTKAVLLKSRDLNVEQG
Sbjct: 181 NFSGLEAKELGLVSRTFDSISKLQDGVLKIAEEIASSSPLAVVGTKAVLLKSRDLNVEQG 240

Query: 241 LDYVATWNSGTLLSDDLKEAISAQANKRKPKFSKL 276
           LDYVATWNSGTLLSDDLKEAISAQA+KRKPKFSKL
Sbjct: 241 LDYVATWNSGTLLSDDLKEAISAQASKRKPKFSKL 275

[Close]
BLAST of Cucsa.095190 vs. NCBI nr
Match: gi|659108122|ref|XP_008454026.1| (PREDICTED: delta(3,5)-Delta(2,4)-dienoyl-CoA isomerase, mitochondrial [Cucumis melo])

HSP 1 Score: 526.9 bits (1356), Expect = 2.1e-146
Identity = 266/275 (96.73%), Postives = 272/275 (98.91%), Query Frame = 1 

Query: 1   MEDYKCIKIERQNPNSGVFHLRLHRPSHYNALTTQLFTELRQAFSHLDQNPDVHVIILSG 60
           ME Y+CIKIERQ+PNSGVFHLRLHRPSHYNALTTQLFTELRQAFS LDQNPDVHVIILSG
Sbjct: 1   MEGYECIKIERQSPNSGVFHLRLHRPSHYNALTTQLFTELRQAFSRLDQNPDVHVIILSG 60

Query: 61  SGKHFCAGIDLKSTASNFEKHLSEERGRAGERIRREIKWMQESITAIEECRKPVIASIHG 120
           SGKHFCAGIDLKSTASNFEKHLSEERGRAGERIRREIKWMQESITAIEECRKPVIASIHG
Sbjct: 61  SGKHFCAGIDLKSTASNFEKHLSEERGRAGERIRREIKWMQESITAIEECRKPVIASIHG 120

Query: 121 GCVGGGIDIVTACDLRYCTAEAVFSVREVKLAITADLGTLQRLPRIVGYGKAAELALTGR 180
           GC+GGGIDIVTACDLRYCTAEAVFSVREVKLAITADLGTLQRLPRIVGYGKAAELALTGR
Sbjct: 121 GCIGGGIDIVTACDLRYCTAEAVFSVREVKLAITADLGTLQRLPRIVGYGKAAELALTGR 180

Query: 181 NFSGLEAKELGLVSRTFDSISKLQDGVLKIAEEIASSSPLAVVGTKAVLLKSRDLNVEQG 240
           +FSGLEAKELGLVSRTFDS+S+LQDGVLKIAEEIAS SPLAVVGTKAVLLKSRDLNVEQG
Sbjct: 181 DFSGLEAKELGLVSRTFDSMSELQDGVLKIAEEIASRSPLAVVGTKAVLLKSRDLNVEQG 240

Query: 241 LDYVATWNSGTLLSDDLKEAISAQANKRKPKFSKL 276
           LDYVATWNSGTLLSDDLKEAISAQANKRKPKFSKL
Sbjct: 241 LDYVATWNSGTLLSDDLKEAISAQANKRKPKFSKL 275

[Close]
BLAST of Cucsa.095190 vs. NCBI nr
Match: gi|694416672|ref|XP_009336455.1| (PREDICTED: delta(3,5)-Delta(2,4)-dienoyl-CoA isomerase, mitochondrial-like [Pyrus x bretschneideri])

HSP 1 Score: 377.9 bits (969), Expect = 1.5e-101
Identity = 191/275 (69.45%), Postives = 223/275 (81.09%), Query Frame = 1 

Query: 1   MEDYKCIKIERQNPNSGVFHLRLHRPSHYNALTTQLFTELRQAFSHLDQNPDVHVIILSG 60
           M  YK ++I ++NP S VF+L L+RPSH NAL+   FTE  +A S LDQNP+V+VI+LSG
Sbjct: 1   MAIYKSLEIFQKNPKSSVFYLHLNRPSHRNALSRDFFTEFPEALSSLDQNPNVNVIVLSG 60

Query: 61  SGKHFCAGIDLKSTASNFEKHLSEERGRAGERIRREIKWMQESITAIEECRKPVIASIHG 120
           +G HFCAGIDLK+  S  EK +S +RGR GE+ RREIKW+Q++ITAIE CRKPVIASIHG
Sbjct: 61  TGDHFCAGIDLKTLTSISEKFVSVDRGRVGEQFRREIKWLQDAITAIERCRKPVIASIHG 120

Query: 121 GCVGGGIDIVTACDLRYCTAEAVFSVREVKLAITADLGTLQRLPRIVGYGKAAELALTGR 180
            C+GGGIDIVTACD+RYC+ +A FSV+EV LAITADLGTLQRLP IVGYG A ELALTGR
Sbjct: 121 ACIGGGIDIVTACDIRYCSKDAFFSVKEVDLAITADLGTLQRLPGIVGYGNAIELALTGR 180

Query: 181 NFSGLEAKELGLVSRTFDSISKLQDGVLKIAEEIASSSPLAVVGTKAVLLKSRDLNVEQG 240
            FSG EAK+LGLVS  FDS  +L++GV  IAE I   SPLAV GTKAVL +SR+LNVEQG
Sbjct: 181 RFSGPEAKQLGLVSGVFDSRDELENGVRLIAEGIGLKSPLAVTGTKAVLQRSRELNVEQG 240

Query: 241 LDYVATWNSGTLLSDDLKEAISAQANKRKPKFSKL 276
           LDYVATWNS  LLSDDL EA+SA A KRKP F+KL
Sbjct: 241 LDYVATWNSAMLLSDDLTEAVSASAQKRKPIFAKL 275

[Close]
BLAST of Cucsa.095190 vs. NCBI nr
Match: gi|657995196|ref|XP_008389923.1| (PREDICTED: delta(3,5)-Delta(2,4)-dienoyl-CoA isomerase, mitochondrial [Malus domestica])

HSP 1 Score: 375.6 bits (963), Expect = 7.7e-101
Identity = 191/275 (69.45%), Postives = 222/275 (80.73%), Query Frame = 1 

Query: 1   MEDYKCIKIERQNPNSGVFHLRLHRPSHYNALTTQLFTELRQAFSHLDQNPDVHVIILSG 60
           M  YK ++I ++NP S VF+L L+RPSH NAL+   FTE  +A S LDQNP+V+VI+LSG
Sbjct: 1   MAIYKSLEIFQKNPKSSVFYLHLNRPSHRNALSRDFFTEFPEALSSLDQNPNVNVIVLSG 60

Query: 61  SGKHFCAGIDLKSTASNFEKHLSEERGRAGERIRREIKWMQESITAIEECRKPVIASIHG 120
           +G HFCAGIDLK+  S  E  +S +RGR GE+ RREIKW+Q++ITAIE CRKPVIASIHG
Sbjct: 61  TGDHFCAGIDLKTLTSISENFVSVDRGRVGEQFRREIKWLQDAITAIERCRKPVIASIHG 120

Query: 121 GCVGGGIDIVTACDLRYCTAEAVFSVREVKLAITADLGTLQRLPRIVGYGKAAELALTGR 180
            C+GGGIDIVTACD+RYC+ +A FSV+EV LAITADLGTLQRLP IVGYG A ELALTGR
Sbjct: 121 ACIGGGIDIVTACDIRYCSKDAFFSVKEVDLAITADLGTLQRLPGIVGYGNAIELALTGR 180

Query: 181 NFSGLEAKELGLVSRTFDSISKLQDGVLKIAEEIASSSPLAVVGTKAVLLKSRDLNVEQG 240
            FSG EAK+LGLVS  FDS  +L++GV  IAE I   SPLAV GTKAVL +SR+LNVEQG
Sbjct: 181 RFSGPEAKQLGLVSGVFDSRDELENGVRLIAEGIGLKSPLAVTGTKAVLQRSRELNVEQG 240

Query: 241 LDYVATWNSGTLLSDDLKEAISAQANKRKPKFSKL 276
           LDYVATWNS  LLSDDL EAISA A KRKP F+KL
Sbjct: 241 LDYVATWNSAMLLSDDLTEAISAFAQKRKPVFAKL 275

[Close]
BLAST of Cucsa.095190 vs. NCBI nr
Match: gi|645231067|ref|XP_008222222.1| (PREDICTED: delta(3,5)-Delta(2,4)-dienoyl-CoA isomerase, mitochondrial [Prunus mume])

HSP 1 Score: 372.1 bits (954), Expect = 8.5e-100
Identity = 187/275 (68.00%), Postives = 222/275 (80.73%), Query Frame = 1 

Query: 1   MEDYKCIKIERQNPNSGVFHLRLHRPSHYNALTTQLFTELRQAFSHLDQNPDVHVIILSG 60
           M +Y  ++I ++NPNS VF+L L+RP+H NAL+   FTE  +A S LDQNPDV+VI+LSG
Sbjct: 1   MANYTTLEIVQENPNSAVFYLYLNRPNHRNALSRDFFTEFPRALSSLDQNPDVNVIVLSG 60

Query: 61  SGKHFCAGIDLKSTASNFEKHLSEERGRAGERIRREIKWMQESITAIEECRKPVIASIHG 120
           +G HFCAGIDLK+  S  ++ +S +RGRAGE+ RREIK +QE+ITAIE CRKPV+ASIHG
Sbjct: 61  AGNHFCAGIDLKTLTSISDQFVSGDRGRAGEKFRREIKELQEAITAIERCRKPVVASIHG 120

Query: 121 GCVGGGIDIVTACDLRYCTAEAVFSVREVKLAITADLGTLQRLPRIVGYGKAAELALTGR 180
            C+GGG+DIVTACD+RYC+ +A FSV+EV LAITADLGTLQRLP IVGYG A ELALTGR
Sbjct: 121 ACIGGGVDIVTACDIRYCSKDAFFSVKEVDLAITADLGTLQRLPSIVGYGNALELALTGR 180

Query: 181 NFSGLEAKELGLVSRTFDSISKLQDGVLKIAEEIASSSPLAVVGTKAVLLKSRDLNVEQG 240
            FS  EAKELGLVSR F S  +L +GV  +AE I   SPLAV GTKAVL +SR+LNVEQG
Sbjct: 181 TFSSSEAKELGLVSRVFGSREELDNGVRVVAEGIGLKSPLAVRGTKAVLQRSRELNVEQG 240

Query: 241 LDYVATWNSGTLLSDDLKEAISAQANKRKPKFSKL 276
           LDYVATWNS  LLSDDL EA+SA A KRKP F+KL
Sbjct: 241 LDYVATWNSAMLLSDDLSEAVSAYAQKRKPVFAKL 275

The following BLAST results are available for this feature:
BLAST of Cucsa.095190 vs. Swiss-Prot
Match NameE-valueIdentityDescription
DCI1_ARATH1.4e-9764.49Delta(3,5)-Delta(2,4)-dienoyl-CoA isomerase, peroxisomal OS=Arabidopsis thaliana... [more]
ECH1_MOUSE8.3e-5539.43Delta(3,5)-Delta(2,4)-dienoyl-CoA isomerase, mitochondrial OS=Mus musculus GN=Ec... [more]
ECH1_RAT3.2e-5439.43Delta(3,5)-Delta(2,4)-dienoyl-CoA isomerase, mitochondrial OS=Rattus norvegicus ... [more]
ECH1_PONAB3.2e-5442.37Delta(3,5)-Delta(2,4)-dienoyl-CoA isomerase, mitochondrial OS=Pongo abelii GN=EC... [more]
ECH1_HUMAN4.1e-5442.75Delta(3,5)-Delta(2,4)-dienoyl-CoA isomerase, mitochondrial OS=Homo sapiens GN=EC... [more]
BLAST of Cucsa.095190 vs. TrEMBL
Match NameE-valueIdentityDescription
A0A0A0KXB8_CUCSA7.4e-15199.64Uncharacterized protein OS=Cucumis sativus GN=Csa_4G011790 PE=3 SV=1[more]
A0A0V0I419_SOLCH1.7e-9966.91Putative delta(3,5)-Delta(2,4)-dienoyl-CoA isomerase, mitochondrial-like OS=Sola... [more]
M5Y9B9_PRUPE2.9e-9968.00Uncharacterized protein OS=Prunus persica GN=PRUPE_ppa009837mg PE=3 SV=1[more]
A0A078H7T1_BRANA1.4e-9665.94BnaA09g16350D protein OS=Brassica napus GN=BnaA09g16350D PE=3 SV=1[more]
W9QLC1_9ROSA1.4e-9665.09Delta(3,5)-Delta(2,4)-dienoyl-CoA isomerase OS=Morus notabilis GN=L484_017491 PE... [more]
BLAST of Cucsa.095190 vs. TAIR10
Match NameE-valueIdentityDescription
AT5G43280.17.6e-9964.49 delta(3,5),delta(2,4)-dienoyl-CoA isomerase 1[more]
AT4G16800.16.4e-2930.11 ATP-dependent caseinolytic (Clp) protease/crotonase family protein[more]
AT4G16210.13.4e-2227.04 enoyl-CoA hydratase/isomerase A[more]
AT4G29010.13.9e-1827.49 Enoyl-CoA hydratase/isomerase family[more]
AT1G60550.12.8e-1630.08 enoyl-CoA hydratase/isomerase D[more]
BLAST of Cucsa.095190 vs. NCBI nr
Match NameE-valueIdentityDescription
gi|449468826|ref|XP_004152122.1|1.1e-15099.64PREDICTED: delta(3,5)-Delta(2,4)-dienoyl-CoA isomerase, mitochondrial [Cucumis s... [more]
gi|659108122|ref|XP_008454026.1|2.1e-14696.73PREDICTED: delta(3,5)-Delta(2,4)-dienoyl-CoA isomerase, mitochondrial [Cucumis m... [more]
gi|694416672|ref|XP_009336455.1|1.5e-10169.45PREDICTED: delta(3,5)-Delta(2,4)-dienoyl-CoA isomerase, mitochondrial-like [Pyru... [more]
gi|657995196|ref|XP_008389923.1|7.7e-10169.45PREDICTED: delta(3,5)-Delta(2,4)-dienoyl-CoA isomerase, mitochondrial [Malus dom... [more]
gi|645231067|ref|XP_008222222.1|8.5e-10068.00PREDICTED: delta(3,5)-Delta(2,4)-dienoyl-CoA isomerase, mitochondrial [Prunus mu... [more]
Annotated Terms
The following terms have been associated with this gene:
Vocabulary: INTERPRO
TermDefinition
IPR001753Enoyl-CoA_hydra/iso
IPR014748Enoyl-CoA_hydra_C
IPR018376Enoyl-CoA_hyd/isom_CS
Vocabulary: Molecular Function
TermDefinition
GO:0003824catalytic activity
Vocabulary: Biological Process
TermDefinition
GO:0008152metabolic process
GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0008152 metabolic process
cellular_component GO:0005777 peroxisome
cellular_component GO:0005575 cellular_component
molecular_function GO:0051750 delta3,5-delta2,4-dienoyl-CoA isomerase activity
molecular_function GO:0003824 catalytic activity
Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Cucsa.095190.1Cucsa.095190.1mRNA


InterPro
Analysis Name: InterPro Annotations of cucumber (Gy14)
Date Performed: 2017-01-17
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001753Crotonase superfamilyPFAMPF00378ECH_1coord: 15..273
score: 1.5
IPR014748Crontonase, C-terminalGENE3DG3DSA:1.10.12.10coord: 218..275
score: 5.3
IPR018376Enoyl-CoA hydratase/isomerase, conserved sitePROSITEPS00166ENOYL_COA_HYDRATASEcoord: 115..135
scor
NoneNo IPR availablePANTHERPTHR11941ENOYL-COA HYDRATASE-RELATEDcoord: 1..272
score: 2.8E
NoneNo IPR availablePANTHERPTHR11941:SF43DELTA(3,5)-DELTA(2,4)-DIENOYL-COA ISOMERASE, MITOCHONDRIALcoord: 1..272
score: 2.8E