Cucsa.090430 (gene) Cucumber (Gy14) v1

NameCucsa.090430
Typegene
OrganismCucumis sativus (Cucumber (Gy14) v1)
DescriptionWAT1-related protein
Locationscaffold00894 : 1730741 .. 1733143 (+)
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGAAGGAGATGATATTACAGCACTGATTGGGATGATTATATTACAAATATGTTATGCTGGTATTAACATCATCTCTAAACTTGCTATGCAATCTGGAATGAACCCTCTTGTTCTTCTCACTTATAGACAGATTTTTGGCACTTTGGCCATTGCTCCTTTTGCCTTTCTTACTGAAAGGTATACCCCTGATTCTTTAATTTCTTTGTTATGGTGTCATTTTCTTTAATAATAATAATAATAATAATAAAaGAATCTTTAGTTTCTTGGTTTATGTTTGATTTTTtCTTTTTTTttGTTTGTCATCTTGTAAAGATTCATGTAATCTTGTGTTATCTTTTtATTTTTtATAATATATTATGAGTTTTtCTGGGTGCAGGAAAACAAGGCCAAAGATCACTTTTGCAGTTTTGTTTCAAATCTTTGTGTGTTCCTTATCAGGGTGAGTCAAAACTCAAAAGGTTTCATCAGTAAGTTTTGAACAATTTTAATTCGATTACGATTTGGGTTTTTTtCTTAGTTTTCAAAATGTTTTGTTTTTtAAAAAaTAAAAaGTAAATAATTATAATTGAGAAATTGAACAAAAGAGCAGATCACCTAACCTAAACATTCTGAATGTTATGGGACGACAGAGCAACAGGGAATCAGATATTCTTCTTTGTTGGGTTAAAGTACACAAACCCAACAATTTCAAGTGCTATGGCCAATGTTCTTCCAGCTGCTACTTTTATTCTTGCTGTTCTCTTCAGGTCATTTTTCTATATGCATCATTTTCGTTCAGTTTTATGTAACGTATAATCCACTTGTTGCATCTAAGAGTCTACAATATATATAGAATGTCATATACTATATCTATTCGATATTTGACACGTCGTACGTCATCATTGAAAATATGTTAATAGTATTTTATGGGTCCAACTATAATTCCATTAAATATTGGAAATTGAGGTAATATGAAAAATGATTGTAGTACTAGAAATTGTAATATAGCTTTATAAATTATTATTATTTTTTGGATTTAGGGGATGTTTTTtAAGAAAAAaTTAAGGGTTTTTtAATTTCTTTTCATTGAAAGGGCTTGATAAATAATGTATATAATATGCAATAATTTATTTGGGCAGACAAGAGAGTGTGAGAATCAAGACCAAATCTGGTTTTGCAAAGGTAATAGGGACAATTGTGTGTGTAAGTGGGGCAATGCTACTGTCTTTCTACCATGGACACACCATCGACTTAGGTGAATCAAGAATTCATTGGTCATATGTTGAGAGATTGATCAAGGAAACAACCCCAACGAATGGTCAAGGAAAGCACGTACTCGGTTCGATTTTGTTGCTTTTGAGCTCTTTTTCATGGGCATTATGGTTTGTCATCCAAGTAAGTAATTACTTTTTAAATCACCCATTACCCTAATTTTGCTACATTTAATTTTCAATCCACTTGAGAAACTTATTATATATACTTTGTAGGCAAGATTGAGTGTCAAGTTCAAAGCTCCCTATACAAGTACTGCATTGTTGTGCTTCATGGCCTTTTTTCAATGTGGACTTATAGCTGTGATTTCAGAGCACAATATTGCTGCTTGGTCTTTGAAAAGTTCAATCCGACTCGTCGCAGCTCTTTACGCAGTATGTACCGTACTTAGTTGTCATTACGATAAACTTTTGAGAAACTATTGGATATATGATATGTTATATGTTGTTTATTGACAGGGAGTTGTGTGTTCTGCATTGACATTTAGTATCACTTCATGGACTATACAAAGGAAAGGCCCTCTCTATGTCTCCATTTTCTCCCCTTTATTGCTTATCATTGTGGCCATAATCAGTTGGGCTCTCCTCCATCAACAACTCCACGCTGGAACGTGCGTATTCTCATGCATTTTTCTTCAATTCTAAACATTATTATCGTTACCAAACGGTCATTTTAACCCTCTTACTCTTTAAAAACGTACATAAAACCTATCATATCTTAGCTTATTAGATTGATGTTTAAAATTTAATCTTAGTCTAAATTCAAGTTTCTAATATGTCTTGTTCGTAGACCCCATTTGAAAGTTATTCCTTTTGTATTTTAGTTTTTATGTTTTTTTATCAACTTTTTCTAATGTTTTAAAAAaCAAACCCCCTTTTtGTTTTtGTTTTTTTTTtAAAAAAAaaTTGATTAGGGATTTAAGTGTTTCTTATCTTCGTCCCATTGAAAATTTTGAAGAAAATAGAAAACTAGAAACAAATTTTGTATCAAGCAGCATGTTCATTtCAACGTTTCAGAGGTAGAATTTTGTGTTAATTTCTAAAATTGTTAGTTATTTGTTGTTTTTTTGGGATTCAACAGTGTTATTGGCTCTGTTTTGATAATCACTGGGCTTTATGCTGTGCTATGGGGAAAATCCAAAGAGATGAAA

mRNA sequence

ATGGAAGGAGATGATATTACAGCACTGATTGGGATGATTATATTACAAATATGTTATGCTGGTATTAACATCATCTCTAAACTTGCTATGCAATCTGGAATGAACCCTCTTGTTCTTCTCACTTATAGACAGATTTTTGGCACTTTGGCCATTGCTCCTTTTGCCTTTCTTACTGAAAGGAAAACAAGGCCAAAGATCACTTTTGCAGTTTTGTTTCAAATCTTTGTGTGTTCCTTATCAGGAGCAACAGGGAATCAGATATTCTTCTTTGTTGGGTTAAAGTACACAAACCCAACAATTTCAAGTGCTATGGCCAATGTTCTTCCAGCTGCTACTTTTATTCTTGCTGTTCTCTTCAGACAAGAGAGTGTGAGAATCAAGACCAAATCTGGTTTTGCAAAGGTAATAGGGACAATTGTGTGTGTAAGTGGGGCAATGCTACTGTCTTTCTACCATGGACACACCATCGACTTAGGTGAATCAAGAATTCATTGGTCATATGTTGAGAGATTGATCAAGGAAACAACCCCAACGAATGGTCAAGGAAAGCACGTACTCGGTTCGATTTTGTTGCTTTTGAGCTCTTTTTCATGGGCATTATGGTTTGTCATCCAAGCAAGATTGAGTGTCAAGTTCAAAGCTCCCTATACAAGTACTGCATTGTTGTGCTTCATGGCCTTTTTTCAATGTGGACTTATAGCTGTGATTTCAGAGCACAATATTGCTGCTTGGTCTTTGAAAAGTTCAATCCGACTCGTCGCAGCTCTTTACGCAGGAGTTGTGTGTTCTGCATTGACATTTAGTATCACTTCATGGACTATACAAAGGAAAGGCCCTCTCTATGTCTCCATTTTCTCCCCTTTATTGCTTATCATTGTGGCCATAATCAGTTGGGCTCTCCTCCATCAACAACTCCACGCTGGAACTGTTATTGGCTCTGTTTTGATAATCACTGGGCTTTATGCTGTGCTATGGGGAAAATCCAAAGAGATGAAA

Coding sequence (CDS)

ATGGAAGGAGATGATATTACAGCACTGATTGGGATGATTATATTACAAATATGTTATGCTGGTATTAACATCATCTCTAAACTTGCTATGCAATCTGGAATGAACCCTCTTGTTCTTCTCACTTATAGACAGATTTTTGGCACTTTGGCCATTGCTCCTTTTGCCTTTCTTACTGAAAGGAAAACAAGGCCAAAGATCACTTTTGCAGTTTTGTTTCAAATCTTTGTGTGTTCCTTATCAGGAGCAACAGGGAATCAGATATTCTTCTTTGTTGGGTTAAAGTACACAAACCCAACAATTTCAAGTGCTATGGCCAATGTTCTTCCAGCTGCTACTTTTATTCTTGCTGTTCTCTTCAGACAAGAGAGTGTGAGAATCAAGACCAAATCTGGTTTTGCAAAGGTAATAGGGACAATTGTGTGTGTAAGTGGGGCAATGCTACTGTCTTTCTACCATGGACACACCATCGACTTAGGTGAATCAAGAATTCATTGGTCATATGTTGAGAGATTGATCAAGGAAACAACCCCAACGAATGGTCAAGGAAAGCACGTACTCGGTTCGATTTTGTTGCTTTTGAGCTCTTTTTCATGGGCATTATGGTTTGTCATCCAAGCAAGATTGAGTGTCAAGTTCAAAGCTCCCTATACAAGTACTGCATTGTTGTGCTTCATGGCCTTTTTTCAATGTGGACTTATAGCTGTGATTTCAGAGCACAATATTGCTGCTTGGTCTTTGAAAAGTTCAATCCGACTCGTCGCAGCTCTTTACGCAGGAGTTGTGTGTTCTGCATTGACATTTAGTATCACTTCATGGACTATACAAAGGAAAGGCCCTCTCTATGTCTCCATTTTCTCCCCTTTATTGCTTATCATTGTGGCCATAATCAGTTGGGCTCTCCTCCATCAACAACTCCACGCTGGAACTGTTATTGGCTCTGTTTTGATAATCACTGGGCTTTATGCTGTGCTATGGGGAAAATCCAAAGAGATGAAA

Protein sequence

MEGDDITALIGMIILQICYAGINIISKLAMQSGMNPLVLLTYRQIFGTLAIAPFAFLTERKTRPKITFAVLFQIFVCSLSGATGNQIFFFVGLKYTNPTISSAMANVLPAATFILAVLFRQESVRIKTKSGFAKVIGTIVCVSGAMLLSFYHGHTIDLGESRIHWSYVERLIKETTPTNGQGKHVLGSILLLLSSFSWALWFVIQARLSVKFKAPYTSTALLCFMAFFQCGLIAVISEHNIAAWSLKSSIRLVAALYAGVVCSALTFSITSWTIQRKGPLYVSIFSPLLLIIVAIISWALLHQQLHAGTVIGSVLIITGLYAVLWGKSKEMK
BLAST of Cucsa.090430 vs. Swiss-Prot
Match: WTR2_ARATH (WAT1-related protein At1g09380 OS=Arabidopsis thaliana GN=At1g09380 PE=2 SV=1)

HSP 1 Score: 411.4 bits (1056), Expect = 9.5e-114
Identity = 191/331 (57.70%), Postives = 254/331 (76.74%), Query Frame = 1

Query: 1   MEGDDITALIGMIILQICYAGINIISKLAMQSGMNPLVLLTYRQIFGTLAIAPFAFLTER 60
           M   D+   + M+++QI YAG+NI SK+AM++GM PL+L+ YRQIF T+A  P AF  ER
Sbjct: 1   MAKSDMLPFLAMVLVQIGYAGMNITSKMAMEAGMKPLILVAYRQIFATIATFPVAFFLER 60

Query: 61  KTRPKITFAVLFQIFVCSLSGATGNQIFFFVGLKYTNPTISSAMANVLPAATFILAVLFR 120
           KTRPKIT  +L Q+F CS++GATGNQ+ +FVGL+ ++PTI+ A+ N+LPA TF+LA +FR
Sbjct: 61  KTRPKITLRILVQVFFCSITGATGNQVLYFVGLQNSSPTIACALTNLLPAVTFLLAAIFR 120

Query: 121 QESVRIKTKSGFAKVIGTIVCVSGAMLLSFYHGHTIDLGESRIHWSYVERLIKETTPTNG 180
           QE+V IK  SG AKVIGT+VCV GAM+LSFYHGHTI +GES+IHW+Y E + K  + ++G
Sbjct: 121 QETVGIKKASGQAKVIGTLVCVIGAMVLSFYHGHTIGIGESKIHWAYAENITKHGS-SSG 180

Query: 181 QGKHVLGSILLLLSSFSWALWFVIQARLSVKFKAPYTSTALLCFMAFFQCGLIAVISEHN 240
                LG  L++ ++ SWA WF+IQ ++S  F APYTST L+C M   QCG IA+IS+H 
Sbjct: 181 HSNFFLGPFLIMAAAVSWAAWFIIQTKMSETFAAPYTSTLLMCLMGSIQCGAIALISDHT 240

Query: 241 IAAWSLKSSIRLVAALYAGVVCSALTFSITSWTIQRKGPLYVSIFSPLLLIIVAIISWAL 300
           I+ WSL S +R ++ALYAGVV SAL F + SW +QRKGPLYVS+FSPLLL++VAI SWAL
Sbjct: 241 ISDWSLSSPLRFISALYAGVVASALAFCLMSWAMQRKGPLYVSVFSPLLLVVVAIFSWAL 300

Query: 301 LHQQLHAGTVIGSVLIITGLYAVLWGKSKEM 332
           L ++L+ GT +GS L++ GLY VLWGK +E+
Sbjct: 301 LEEKLYTGTFMGSALVVIGLYGVLWGKDREV 330

BLAST of Cucsa.090430 vs. Swiss-Prot
Match: WTR38_ARATH (WAT1-related protein At5g07050 OS=Arabidopsis thaliana GN=At5g07050 PE=2 SV=1)

HSP 1 Score: 264.6 bits (675), Expect = 1.4e-69
Identity = 137/322 (42.55%), Postives = 212/322 (65.84%), Query Frame = 1

Query: 12  MIILQICYAGINIISKLAMQSGMNPLVLLTYRQIFGTLAIAPFAFLTERKTRPKITFAVL 71
           MI LQ  YAG+NII+K+++ +GM+  VL+ YR    T  IAPFAF  ERK +PKITF++ 
Sbjct: 22  MISLQFGYAGMNIITKISLNTGMSHYVLVVYRHAIATAVIAPFAFFFERKAQPKITFSIF 81

Query: 72  FQIFVCSLSGATGNQIFFFVGLKYTNPTISSAMANVLPAATFILAVLFRQESVRIKTKSG 131
            Q+F+  L G   +Q F+++GLKYT+PT S AM+N+LPA TFILAVLFR E + +K    
Sbjct: 82  MQLFILGLLGPVIDQNFYYMGLKYTSPTFSCAMSNMLPAMTFILAVLFRMEMLDLKKLWC 141

Query: 132 FAKVIGTIVCVSGAMLLSFYHGHTIDLGESR-IHWSYVERLIKETTPTNGQGKHVL-GSI 191
            AK+ GT+V V+GAML++ Y G  ++L  ++ +H          ++  +   K  L GSI
Sbjct: 142 QAKIAGTVVTVAGAMLMTIYKGPIVELFWTKYMHIQDSSHANTTSSKNSSSDKEFLKGSI 201

Query: 192 LLLLSSFSWALWFVIQAR-LSVKFKAPYTSTALLCFMAFFQCGLIAVISEHNIAAWSLKS 251
           LL+ ++ +WA  FV+QA+ L    K   + T L+CF+   Q   +  + EHN +AW +  
Sbjct: 202 LLIFATLAWASLFVLQAKILKTYAKHQLSLTTLICFIGTLQAVAVTFVMEHNPSAWRIGW 261

Query: 252 SIRLVAALYAGVVCSALTFSITSWTIQRKGPLYVSIFSPLLLIIVAIISWALLHQQLHAG 311
            + L+AA Y+G+V S++++ +    ++++GP++ + FSPL+++IVA++   +L +++  G
Sbjct: 262 DMNLLAAAYSGIVASSISYYVQGIVMKKRGPVFATAFSPLMMVIVAVMGSFVLAEKIFLG 321

Query: 312 TVIGSVLIITGLYAVLWGKSKE 331
            VIG+VLI+ GLYAVLWGK KE
Sbjct: 322 GVIGAVLIVIGLYAVLWGKQKE 343

BLAST of Cucsa.090430 vs. Swiss-Prot
Match: WTR24_ARATH (WAT1-related protein At3g30340 OS=Arabidopsis thaliana GN=At3g30340 PE=2 SV=1)

HSP 1 Score: 257.7 bits (657), Expect = 1.8e-67
Identity = 125/323 (38.70%), Postives = 209/323 (64.71%), Query Frame = 1

Query: 8   ALIGMIILQICYAGINIISKLAMQSGMNPLVLLTYRQIFGTLAIAPFAFLTERKTRPKIT 67
           A++ M ++ I  + +N++ K  +  G+N +V  TYR   GTL + PFA   ER  RPK+T
Sbjct: 11  AVLMMSMINIGLSVVNVMFKKMIDEGLNRMVATTYRLAVGTLFLIPFAIFLERHNRPKLT 70

Query: 68  FAVLFQIFVCSLSGATGNQIFFFVGLKYTNPTISSAMANVLPAATFILAVLFRQESVRIK 127
             +L  +F  +L G +  Q FF +GL+YT+ T S A +N++P+ TF LA++FRQE++ IK
Sbjct: 71  GRILCSLFFSALLGTSLVQYFFLIGLEYTSSTFSLAFSNMVPSVTFALALVFRQETLNIK 130

Query: 128 TKSGFAKVIGTIVCVSGAMLLSFYHGHTIDLGESRIHWSYVERLIKETTPTNGQGKHVLG 187
           +  G AK++GT++C+ GA++L+ Y G  +    SR H +++E   +  +      K  +G
Sbjct: 131 SNVGRAKLLGTMICICGALVLTLYKGTAL----SREHSTHMETHTRTDSTGAMTQKWAMG 190

Query: 188 SILLLLSSFSWALWFVIQARLSVKFKAPYTSTALLCFMAFFQCGLIAVISEHNIAAWSLK 247
           SI+L++S   W+ WF++QA++S  +   YTST +L F    Q  L+++ISE + + W +K
Sbjct: 191 SIMLVISIIIWSSWFIVQAKISRVYPCQYTSTTILSFFGVIQSALLSLISERSTSMWVVK 250

Query: 248 SSIRLVAALYAGVVCSALTFSITSWTIQRKGPLYVSIFSPLLLIIVAIISWALLHQQLHA 307
              +++A LY+G+V S L +   SW ++++G ++ S F PL+ +  AI S++ LH+Q++ 
Sbjct: 251 DKFQVLALLYSGIVGSGLCYVGMSWCLRQRGAVFTSSFIPLIQVFAAIFSFSFLHEQIYC 310

Query: 308 GTVIGSVLIITGLYAVLWGKSKE 331
           G+VIGS++II GLY +LWGKSK+
Sbjct: 311 GSVIGSMVIIVGLYILLWGKSKD 329

BLAST of Cucsa.090430 vs. Swiss-Prot
Match: WTR15_ARATH (WAT1-related protein At2g40900 OS=Arabidopsis thaliana GN=At2g40900 PE=2 SV=1)

HSP 1 Score: 248.4 bits (633), Expect = 1.1e-64
Identity = 127/316 (40.19%), Postives = 204/316 (64.56%), Query Frame = 1

Query: 12  MIILQICYAGINIISKLAMQSGMNPLVLLTYRQIFGTLAIAPFAFLTERKTRPKITFAVL 71
           M+ LQ  YAG+N+++K  +  GM+  VL+ YR  F T AIAPFA L+ERK R K+TF + 
Sbjct: 15  MVCLQFGYAGMNLVTKTVLDRGMSHYVLVAYRNAFATAAIAPFALLSERKVRSKMTFPIF 74

Query: 72  FQIFVCSLSGATGNQIFFFVGLKYTNPTISSAMANVLPAATFILAVLFRQESVRIKTKSG 131
            +IF+ +L G   +Q  +++GLK T+PT SSA++N++PA T ILA LFR E V ++    
Sbjct: 75  MRIFLLALLGPVIDQNLYYIGLKLTSPTFSSAVSNIVPAITIILATLFRMEKVEMRKVRC 134

Query: 132 FAKVIGTIVCVSGAMLLSFYHGHTIDLGESRIHWSYVERLIKETTPTNGQGKHVLGSILL 191
             KV+GT+V V G++L+ FY G  I+   S +           + PT     ++  ++ L
Sbjct: 135 LVKVMGTLVTVVGSILMIFYKGPFINFFRSHL-------TAASSPPT---ADYLKAAVFL 194

Query: 192 LLSSFSWALWFVIQARLSVKFKAPYTSTALLCFMAFFQCGLIAVISEHNIAAWSLKSSIR 251
           LL+S SWA +FV+QA    K+ A  + + ++CFM   Q   +A + EHN +A ++   + 
Sbjct: 195 LLASLSWASFFVLQAATLKKYSAHLSMSTMVCFMGTLQSLALAFVMEHNPSALNIGFDMN 254

Query: 252 LVAALYAGVVCSALTFSITSWTIQRKGPLYVSIFSPLLLIIVAIISWALLHQQLHAGTVI 311
           L+A+ YAG++ S++ + +    +QRKGP++V+ F+PL+++IV+I+S+ +L Q ++ G VI
Sbjct: 255 LLASAYAGIMSSSIAYYVQGLMMQRKGPVFVTAFNPLIVVIVSIMSFFVLGQGIYLGGVI 314

Query: 312 GSVLIITGLYAVLWGK 328
           G V+++ G+YAVLWGK
Sbjct: 315 GVVVLMVGVYAVLWGK 320

BLAST of Cucsa.090430 vs. Swiss-Prot
Match: WTR1_ARATH (WAT1-related protein At1g01070 OS=Arabidopsis thaliana GN=At1g01070 PE=2 SV=1)

HSP 1 Score: 246.1 bits (627), Expect = 5.3e-64
Identity = 126/323 (39.01%), Postives = 192/323 (59.44%), Query Frame = 1

Query: 9   LIGMIILQICYAGINIISKLAMQSGMNPLVLLTYRQIFGTLAIAPFAFLTERKTRPKITF 68
           +I M++  +    +N + K A+  G+N +V+  YR     L + PFA++ ERKTRP+ITF
Sbjct: 17  VIVMVMSNVAMGSVNALVKKALDVGVNHMVIGAYRMAISALILVPFAYVLERKTRPQITF 76

Query: 69  AVLFQIFVCSLSGATGNQIFFFVGLKYTNPTISSAMANVLPAATFILAVLFRQESVRI-K 128
            ++   FV  L GA+  Q FF +GL YT+ T+S A+ ++LPA TF LA++FR E+V+I K
Sbjct: 77  RLMVDHFVSGLLGASLMQFFFLLGLSYTSATVSCALVSMLPAITFALALIFRTENVKILK 136

Query: 129 TKSGFAKVIGTIVCVSGAMLLSFYHGHTIDLGESRIHWSYVERLIKETTPTNGQGKHVLG 188
           TK+G  KVIGT++C+SGA+ L+FY G  I    S  H         +    N     +LG
Sbjct: 137 TKAGMLKVIGTLICISGALFLTFYKGPQISNSHSHSHGGASHNNNDQDKANNW----LLG 196

Query: 189 SILLLLSSFSWALWFVIQARLSVKFKAPYTSTALLCFMAFFQCGLIAVISEHNIAAWSLK 248
            + L + +   +LW + Q  LS+K+   Y+ST L+   A FQC L+++    ++  W + 
Sbjct: 197 CLYLTIGTVLLSLWMLFQGTLSIKYPCKYSSTCLMSIFAAFQCALLSLYKSRDVNDWIID 256

Query: 249 SSIRLVAALYAGVVCSALTFSITSWTIQRKGPLYVSIFSPLLLIIVAIISWALLHQQLHA 308
               +   +YAGVV  A+T   T+W I++ G ++ S F PL LI   +  + +LH  L+ 
Sbjct: 257 DRFVITVIIYAGVVGQAMTTVATTWGIKKLGAVFASAFFPLTLISATLFDFLILHTPLYL 316

Query: 309 GTVIGSVLIITGLYAVLWGKSKE 331
           G+VIGS++ ITGLY  LWGK+KE
Sbjct: 317 GSVIGSLVTITGLYMFLWGKNKE 335

BLAST of Cucsa.090430 vs. TrEMBL
Match: A0A0A0LW49_CUCSA (WAT1-related protein OS=Cucumis sativus GN=Csa_1G181350 PE=3 SV=1)

HSP 1 Score: 429.1 bits (1102), Expect = 4.9e-117
Identity = 212/331 (64.05%), Postives = 265/331 (80.06%), Query Frame = 1

Query: 3   GDDITALIGMIILQICYAGINIISKLAMQSGMNPLVLLTYRQIFGTLAIAPFAFLTERKT 62
           GD +  L+ M+++Q  YAG+NI SKLAM+ GMNPLVL+ YRQ+F T+AIAP A+  ERK 
Sbjct: 5   GDFLPPLV-MLVVQFLYAGLNITSKLAMEFGMNPLVLVAYRQMFATIAIAPCAYWFERKG 64

Query: 63  RPKITFAVLFQIFVCSLSGATGNQIFFFVGLKYTNPTISSAMANVLPAATFILAVLFRQE 122
           RPKIT  +LFQI +CSL+GAT NQ+F++VGLKY+ PTI+ A+ NVLPAATF+LAVLFRQE
Sbjct: 65  RPKITKPILFQILLCSLTGATANQVFYYVGLKYSTPTIACALTNVLPAATFVLAVLFRQE 124

Query: 123 SVRIKTKSGFAKVIGTIVCVSGAMLLSFYHGHTIDLGESRIHWSYVERLIKETTPTNGQG 182
           SV IKT  G AKVIGT+VCV GAMLLSFY G TI+LG+S IHW Y E +  E+  ++ QG
Sbjct: 125 SVGIKTSPGAAKVIGTVVCVGGAMLLSFYRGQTIELGKSGIHWKYAELMRGES--SSNQG 184

Query: 183 KHVLGSILLLLSSFSWALWFVIQARLSVKFKAPYTSTALLCFMAFFQCGLIAVISEH-NI 242
             + GS+ L++SS +WA WFVIQAR++ KF APYTSTAL+ FMA  QCG IAV  EH  +
Sbjct: 185 SSIWGSLCLIISSVAWAAWFVIQARVNEKFPAPYTSTALMTFMATIQCGAIAVGVEHKTL 244

Query: 243 AAWSLKSSIRLVAALYAGVVCSALTFSITSWTIQRKGPLYVSIFSPLLLIIVAIISWALL 302
           AAWSLKSSIRLV ALYAGV CS + F +TSW+IQ++GPLY S+FSP LL+IVAI SWA  
Sbjct: 245 AAWSLKSSIRLVGALYAGVACSGMAFCLTSWSIQKRGPLYASVFSPFLLVIVAIFSWAFF 304

Query: 303 HQQLHAGTVIGSVLIITGLYAVLWGKSKEMK 333
            ++L+ GTV+GS+LI+ GLY+VLWGK+KE+K
Sbjct: 305 QEKLYVGTVVGSLLIVVGLYSVLWGKTKEVK 332

BLAST of Cucsa.090430 vs. TrEMBL
Match: M5W6P9_PRUPE (WAT1-related protein (Fragment) OS=Prunus persica GN=PRUPE_ppa018179mg PE=3 SV=1)

HSP 1 Score: 427.2 bits (1097), Expect = 1.9e-116
Identity = 198/326 (60.74%), Postives = 264/326 (80.98%), Query Frame = 1

Query: 5   DITALIGMIILQICYAGINIISKLAMQSGMNPLVLLTYRQIFGTLAIAPFAFLTERKTRP 64
           D    + M+++Q+ YAG+NIISKLA++S MNPLVL+ YRQ+F TL+IAPFA+  E KTRP
Sbjct: 6   DYLPFLAMVLVQMSYAGMNIISKLAIESDMNPLVLVAYRQVFATLSIAPFAYWMEWKTRP 65

Query: 65  KITFAVLFQIFVCSLSGATGNQIFFFVGLKYTNPTISSAMANVLPAATFILAVLFRQESV 124
           +IT  +LFQ F+CSL+GAT NQ+F+FVGLK + PTI+ A+ N LPA TFILA++FRQES 
Sbjct: 66  RITMPILFQTFLCSLTGATANQVFYFVGLKTSTPTIACALTNTLPAMTFILALIFRQESA 125

Query: 125 RIKTKSGFAKVIGTIVCVSGAMLLSFYHGHTIDLGESRIHWSYVERLIKETTPTNGQGKH 184
           +IK+K G +KV+GT+VCVSGAMLLSFYHGH I LGES+IHW+Y +R+  E   ++  G  
Sbjct: 126 KIKSKPGLSKVMGTVVCVSGAMLLSFYHGHIIGLGESKIHWAYAQRM-GEQANSSSNGSS 185

Query: 185 VLGSILLLLSSFSWALWFVIQARLSVKFKAPYTSTALLCFMAFFQCGLIAVISEHNIAAW 244
            +G + +++S+  WA WF+IQA++   F APYTST L+C MA F+CG+IAVI++H ++AW
Sbjct: 186 FVGPLCVIISTLGWAFWFIIQAKVGENFPAPYTSTTLMCLMASFECGIIAVIADHKVSAW 245

Query: 245 SLKSSIRLVAALYAGVVCSALTFSITSWTIQRKGPLYVSIFSPLLLIIVAIISWALLHQQ 304
           SLK+ +RL++ALY G++ SAL F ++SW+IQRKGPLYVS+FSPLLLIIVAI SWALL ++
Sbjct: 246 SLKNPMRLISALYCGILGSALAFFLSSWSIQRKGPLYVSVFSPLLLIIVAISSWALLEEK 305

Query: 305 LHAGTVIGSVLIITGLYAVLWGKSKE 331
           L+ GT IGS+LI+ GLY VLWGK+KE
Sbjct: 306 LYLGTAIGSILIVCGLYLVLWGKNKE 330

BLAST of Cucsa.090430 vs. TrEMBL
Match: W9R2P6_9ROSA (WAT1-related protein OS=Morus notabilis GN=L484_018875 PE=3 SV=1)

HSP 1 Score: 425.6 bits (1093), Expect = 5.4e-116
Identity = 198/330 (60.00%), Postives = 264/330 (80.00%), Query Frame = 1

Query: 3   GDDITALIGMIILQICYAGINIISKLAMQSGMNPLVLLTYRQIFGTLAIAPFAFLTERKT 62
           GD +  L+ M+ +Q+CYAG+NI SK+A++SGMNPLVL+ YRQIF T+AI PF+++ E KT
Sbjct: 9   GDCVPFLV-MVFVQVCYAGMNITSKVALESGMNPLVLVAYRQIFATMAIGPFSYIMEWKT 68

Query: 63  RPKITFAVLFQIFVCSLSGATGNQIFFFVGLKYTNPTISSAMANVLPAATFILAVLFRQE 122
            PKITF +LFQIF+CSL+GA  NQ+F+F+GLKY++  I+SA++N+LPA TF+LAV+FRQE
Sbjct: 69  MPKITFPILFQIFLCSLTGAVANQVFYFLGLKYSSAIIASALSNILPAVTFLLAVIFRQE 128

Query: 123 SVRIKTKSGFAKVIGTIVCVSGAMLLSFYHGHTIDLGESRIHWSYVERLIKETTPTNGQG 182
           S  I+ KSG AK++GTI+CV GAM+LSFY G  I LGE  ++WSY++++ + TT      
Sbjct: 129 SAEIRKKSGIAKIVGTILCVGGAMMLSFYQGKVIGLGEPNMNWSYLQKVRESTTSNTVNS 188

Query: 183 KHVLGSILLLLSSFSWALWFVIQARLSVKFKAPYTSTALLCFMAFFQCGLIAVISEHNIA 242
             +LG I ++LS+  WA WF+IQA +S KF APYTST L+CFMA  +CG I  + EH I+
Sbjct: 189 NPLLGPIFIILSTVGWAAWFIIQAGMSEKFPAPYTSTTLMCFMATVECGAIGFVLEHKIS 248

Query: 243 AWSLKSSIRLVAALYAGVVCSALTFSITSWTIQRKGPLYVSIFSPLLLIIVAIISWALLH 302
           AWSL++  RLVA+LYAG++ SA+ F +TSW+IQRKGPLYVS+FSPLLLIIVAI+SWALL 
Sbjct: 249 AWSLRNDTRLVASLYAGIMGSAIAFFLTSWSIQRKGPLYVSVFSPLLLIIVAILSWALLK 308

Query: 303 QQLHAGTVIGSVLIITGLYAVLWGKSKEMK 333
           ++++ GTV+GS  II GLYAVLWGK KEM+
Sbjct: 309 EKIYIGTVLGSTFIIAGLYAVLWGKGKEME 337

BLAST of Cucsa.090430 vs. TrEMBL
Match: A0A0A0LGK0_CUCSA (WAT1-related protein OS=Cucumis sativus GN=Csa_2G034730 PE=3 SV=1)

HSP 1 Score: 425.2 bits (1092), Expect = 7.1e-116
Identity = 222/249 (89.16%), Postives = 227/249 (91.16%), Query Frame = 1

Query: 82  ATGNQIFFFVGLKYTNPTISSAMANVLPAATFILAVLFRQESVRIKTKSGFAKVIGTIVC 141
           ATGNQIFFFVGLKYTNPTISSAMANVLPAATFILAVLFRQESVRIKTKSGFAKVIGTIVC
Sbjct: 7   ATGNQIFFFVGLKYTNPTISSAMANVLPAATFILAVLFRQESVRIKTKSGFAKVIGTIVC 66

Query: 142 VSGAMLLSFYHGHTIDLGESRIHWSYVERLIKETTPTNGQGKHVLGSILLLLSSFSWALW 201
           VSGAMLLSFYHGHTIDLGESRIHWSYVERLIKETTPTNGQGKHVLGSILLLLSSFSWALW
Sbjct: 67  VSGAMLLSFYHGHTIDLGESRIHWSYVERLIKETTPTNGQGKHVLGSILLLLSSFSWALW 126

Query: 202 FVIQARLSVKFKAPYTSTALLCFMAFFQCGLIAVISEHNIAAWSLKSSIRLVAALYAGVV 261
           FVIQARLSVKFKAPYTSTALLCFMAFFQCGLIAVISEHNIAAWSLKSSIRLVAALYA  V
Sbjct: 127 FVIQARLSVKFKAPYTSTALLCFMAFFQCGLIAVISEHNIAAWSLKSSIRLVAALYA--V 186

Query: 262 CSALTFS-------------ITSWTIQRKGPLYVSIFSPLLLIIVAIISWALLHQQLHAG 318
           C+ L+               ITSWTIQRKGPLYVSIFSPLLLIIVAIISWALLHQQLHAG
Sbjct: 187 CTVLSCHYDKLLRNYWIYDIITSWTIQRKGPLYVSIFSPLLLIIVAIISWALLHQQLHAG 246

BLAST of Cucsa.090430 vs. TrEMBL
Match: A0A061F4Z0_THECC (WAT1-related protein OS=Theobroma cacao GN=TCM_026803 PE=3 SV=1)

HSP 1 Score: 423.3 bits (1087), Expect = 2.7e-115
Identity = 199/328 (60.67%), Postives = 265/328 (80.79%), Query Frame = 1

Query: 5   DITALIGMIILQICYAGINIISKLAMQSGMNPLVLLTYRQIFGTLAIAPFAFLTERKTRP 64
           D    +  +++Q+ YAG+NI SKLAM+SGM PL+L+ YRQIF TLAIAPFA+  ERKTRP
Sbjct: 4   DFVPFLANVLVQLGYAGMNITSKLAMESGMKPLILVAYRQIFATLAIAPFAYFLERKTRP 63

Query: 65  KITFAVLFQIFVCSLSGATGNQIFFFVGLKYTNPTISSAMANVLPAATFILAVLFRQESV 124
           KIT  +LFQIF+CSL+GAT NQ+F+FVGL+ ++ T++ A+ NVLPAATF LA L RQE+V
Sbjct: 64  KITKHILFQIFLCSLTGATANQVFYFVGLENSSATVACALNNVLPAATFALAALCRQEAV 123

Query: 125 RIKTKSGFAKVIGTIVCVSGAMLLSFYHGHTIDLGESRIHWSYVERLIKETTPTNGQGKH 184
            IK  SG AKV+GT+VCV GAMLLSFYHGHTI +G+S IHW+Y +++  +++ +NG    
Sbjct: 124 GIKKASGQAKVLGTLVCVGGAMLLSFYHGHTIGIGDSSIHWAYADKMTSKSS-SNG-SNF 183

Query: 185 VLGSILLLLSSFSWALWFVIQARLSVKFKAPYTSTALLCFMAFFQCGLIAVISEHNIAAW 244
            LG  L++ S+ +WA+W +IQ + S  F APYT TAL+CFMA  +C +I + S+H I+AW
Sbjct: 184 FLGPFLVMASAVAWAVWLIIQGQTSKNFPAPYTCTALMCFMASIECTIIGIFSDHKISAW 243

Query: 245 SLKSSIRLVAALYAGVVCSALTFSITSWTIQRKGPLYVSIFSPLLLIIVAIISWALLHQQ 304
           SL SS+RL+AALYAG+VC+A+TF + SW+IQ+KGPLYVS+FSPLLL+IVA++SWALL ++
Sbjct: 244 SLSSSMRLIAALYAGIVCNAMTFCVLSWSIQKKGPLYVSVFSPLLLVIVAVLSWALLREK 303

Query: 305 LHAGTVIGSVLIITGLYAVLWGKSKEMK 333
           L+ GTV+GSVLI+ GLYAVLWGK +E+K
Sbjct: 304 LYVGTVVGSVLIVGGLYAVLWGKDREIK 329

BLAST of Cucsa.090430 vs. TAIR10
Match: AT1G09380.1 (AT1G09380.1 nodulin MtN21 /EamA-like transporter family protein)

HSP 1 Score: 411.4 bits (1056), Expect = 5.4e-115
Identity = 191/331 (57.70%), Postives = 254/331 (76.74%), Query Frame = 1

Query: 1   MEGDDITALIGMIILQICYAGINIISKLAMQSGMNPLVLLTYRQIFGTLAIAPFAFLTER 60
           M   D+   + M+++QI YAG+NI SK+AM++GM PL+L+ YRQIF T+A  P AF  ER
Sbjct: 1   MAKSDMLPFLAMVLVQIGYAGMNITSKMAMEAGMKPLILVAYRQIFATIATFPVAFFLER 60

Query: 61  KTRPKITFAVLFQIFVCSLSGATGNQIFFFVGLKYTNPTISSAMANVLPAATFILAVLFR 120
           KTRPKIT  +L Q+F CS++GATGNQ+ +FVGL+ ++PTI+ A+ N+LPA TF+LA +FR
Sbjct: 61  KTRPKITLRILVQVFFCSITGATGNQVLYFVGLQNSSPTIACALTNLLPAVTFLLAAIFR 120

Query: 121 QESVRIKTKSGFAKVIGTIVCVSGAMLLSFYHGHTIDLGESRIHWSYVERLIKETTPTNG 180
           QE+V IK  SG AKVIGT+VCV GAM+LSFYHGHTI +GES+IHW+Y E + K  + ++G
Sbjct: 121 QETVGIKKASGQAKVIGTLVCVIGAMVLSFYHGHTIGIGESKIHWAYAENITKHGS-SSG 180

Query: 181 QGKHVLGSILLLLSSFSWALWFVIQARLSVKFKAPYTSTALLCFMAFFQCGLIAVISEHN 240
                LG  L++ ++ SWA WF+IQ ++S  F APYTST L+C M   QCG IA+IS+H 
Sbjct: 181 HSNFFLGPFLIMAAAVSWAAWFIIQTKMSETFAAPYTSTLLMCLMGSIQCGAIALISDHT 240

Query: 241 IAAWSLKSSIRLVAALYAGVVCSALTFSITSWTIQRKGPLYVSIFSPLLLIIVAIISWAL 300
           I+ WSL S +R ++ALYAGVV SAL F + SW +QRKGPLYVS+FSPLLL++VAI SWAL
Sbjct: 241 ISDWSLSSPLRFISALYAGVVASALAFCLMSWAMQRKGPLYVSVFSPLLLVVVAIFSWAL 300

Query: 301 LHQQLHAGTVIGSVLIITGLYAVLWGKSKEM 332
           L ++L+ GT +GS L++ GLY VLWGK +E+
Sbjct: 301 LEEKLYTGTFMGSALVVIGLYGVLWGKDREV 330

BLAST of Cucsa.090430 vs. TAIR10
Match: AT5G07050.1 (AT5G07050.1 nodulin MtN21 /EamA-like transporter family protein)

HSP 1 Score: 264.6 bits (675), Expect = 8.1e-71
Identity = 137/322 (42.55%), Postives = 212/322 (65.84%), Query Frame = 1

Query: 12  MIILQICYAGINIISKLAMQSGMNPLVLLTYRQIFGTLAIAPFAFLTERKTRPKITFAVL 71
           MI LQ  YAG+NII+K+++ +GM+  VL+ YR    T  IAPFAF  ERK +PKITF++ 
Sbjct: 22  MISLQFGYAGMNIITKISLNTGMSHYVLVVYRHAIATAVIAPFAFFFERKAQPKITFSIF 81

Query: 72  FQIFVCSLSGATGNQIFFFVGLKYTNPTISSAMANVLPAATFILAVLFRQESVRIKTKSG 131
            Q+F+  L G   +Q F+++GLKYT+PT S AM+N+LPA TFILAVLFR E + +K    
Sbjct: 82  MQLFILGLLGPVIDQNFYYMGLKYTSPTFSCAMSNMLPAMTFILAVLFRMEMLDLKKLWC 141

Query: 132 FAKVIGTIVCVSGAMLLSFYHGHTIDLGESR-IHWSYVERLIKETTPTNGQGKHVL-GSI 191
            AK+ GT+V V+GAML++ Y G  ++L  ++ +H          ++  +   K  L GSI
Sbjct: 142 QAKIAGTVVTVAGAMLMTIYKGPIVELFWTKYMHIQDSSHANTTSSKNSSSDKEFLKGSI 201

Query: 192 LLLLSSFSWALWFVIQAR-LSVKFKAPYTSTALLCFMAFFQCGLIAVISEHNIAAWSLKS 251
           LL+ ++ +WA  FV+QA+ L    K   + T L+CF+   Q   +  + EHN +AW +  
Sbjct: 202 LLIFATLAWASLFVLQAKILKTYAKHQLSLTTLICFIGTLQAVAVTFVMEHNPSAWRIGW 261

Query: 252 SIRLVAALYAGVVCSALTFSITSWTIQRKGPLYVSIFSPLLLIIVAIISWALLHQQLHAG 311
            + L+AA Y+G+V S++++ +    ++++GP++ + FSPL+++IVA++   +L +++  G
Sbjct: 262 DMNLLAAAYSGIVASSISYYVQGIVMKKRGPVFATAFSPLMMVIVAVMGSFVLAEKIFLG 321

Query: 312 TVIGSVLIITGLYAVLWGKSKE 331
            VIG+VLI+ GLYAVLWGK KE
Sbjct: 322 GVIGAVLIVIGLYAVLWGKQKE 343

BLAST of Cucsa.090430 vs. TAIR10
Match: AT3G30340.1 (AT3G30340.1 nodulin MtN21 /EamA-like transporter family protein)

HSP 1 Score: 257.7 bits (657), Expect = 9.9e-69
Identity = 125/323 (38.70%), Postives = 209/323 (64.71%), Query Frame = 1

Query: 8   ALIGMIILQICYAGINIISKLAMQSGMNPLVLLTYRQIFGTLAIAPFAFLTERKTRPKIT 67
           A++ M ++ I  + +N++ K  +  G+N +V  TYR   GTL + PFA   ER  RPK+T
Sbjct: 11  AVLMMSMINIGLSVVNVMFKKMIDEGLNRMVATTYRLAVGTLFLIPFAIFLERHNRPKLT 70

Query: 68  FAVLFQIFVCSLSGATGNQIFFFVGLKYTNPTISSAMANVLPAATFILAVLFRQESVRIK 127
             +L  +F  +L G +  Q FF +GL+YT+ T S A +N++P+ TF LA++FRQE++ IK
Sbjct: 71  GRILCSLFFSALLGTSLVQYFFLIGLEYTSSTFSLAFSNMVPSVTFALALVFRQETLNIK 130

Query: 128 TKSGFAKVIGTIVCVSGAMLLSFYHGHTIDLGESRIHWSYVERLIKETTPTNGQGKHVLG 187
           +  G AK++GT++C+ GA++L+ Y G  +    SR H +++E   +  +      K  +G
Sbjct: 131 SNVGRAKLLGTMICICGALVLTLYKGTAL----SREHSTHMETHTRTDSTGAMTQKWAMG 190

Query: 188 SILLLLSSFSWALWFVIQARLSVKFKAPYTSTALLCFMAFFQCGLIAVISEHNIAAWSLK 247
           SI+L++S   W+ WF++QA++S  +   YTST +L F    Q  L+++ISE + + W +K
Sbjct: 191 SIMLVISIIIWSSWFIVQAKISRVYPCQYTSTTILSFFGVIQSALLSLISERSTSMWVVK 250

Query: 248 SSIRLVAALYAGVVCSALTFSITSWTIQRKGPLYVSIFSPLLLIIVAIISWALLHQQLHA 307
              +++A LY+G+V S L +   SW ++++G ++ S F PL+ +  AI S++ LH+Q++ 
Sbjct: 251 DKFQVLALLYSGIVGSGLCYVGMSWCLRQRGAVFTSSFIPLIQVFAAIFSFSFLHEQIYC 310

Query: 308 GTVIGSVLIITGLYAVLWGKSKE 331
           G+VIGS++II GLY +LWGKSK+
Sbjct: 311 GSVIGSMVIIVGLYILLWGKSKD 329

BLAST of Cucsa.090430 vs. TAIR10
Match: AT2G40900.1 (AT2G40900.1 nodulin MtN21 /EamA-like transporter family protein)

HSP 1 Score: 248.4 bits (633), Expect = 6.0e-66
Identity = 127/316 (40.19%), Postives = 204/316 (64.56%), Query Frame = 1

Query: 12  MIILQICYAGINIISKLAMQSGMNPLVLLTYRQIFGTLAIAPFAFLTERKTRPKITFAVL 71
           M+ LQ  YAG+N+++K  +  GM+  VL+ YR  F T AIAPFA L+ERK R K+TF + 
Sbjct: 15  MVCLQFGYAGMNLVTKTVLDRGMSHYVLVAYRNAFATAAIAPFALLSERKVRSKMTFPIF 74

Query: 72  FQIFVCSLSGATGNQIFFFVGLKYTNPTISSAMANVLPAATFILAVLFRQESVRIKTKSG 131
            +IF+ +L G   +Q  +++GLK T+PT SSA++N++PA T ILA LFR E V ++    
Sbjct: 75  MRIFLLALLGPVIDQNLYYIGLKLTSPTFSSAVSNIVPAITIILATLFRMEKVEMRKVRC 134

Query: 132 FAKVIGTIVCVSGAMLLSFYHGHTIDLGESRIHWSYVERLIKETTPTNGQGKHVLGSILL 191
             KV+GT+V V G++L+ FY G  I+   S +           + PT     ++  ++ L
Sbjct: 135 LVKVMGTLVTVVGSILMIFYKGPFINFFRSHL-------TAASSPPT---ADYLKAAVFL 194

Query: 192 LLSSFSWALWFVIQARLSVKFKAPYTSTALLCFMAFFQCGLIAVISEHNIAAWSLKSSIR 251
           LL+S SWA +FV+QA    K+ A  + + ++CFM   Q   +A + EHN +A ++   + 
Sbjct: 195 LLASLSWASFFVLQAATLKKYSAHLSMSTMVCFMGTLQSLALAFVMEHNPSALNIGFDMN 254

Query: 252 LVAALYAGVVCSALTFSITSWTIQRKGPLYVSIFSPLLLIIVAIISWALLHQQLHAGTVI 311
           L+A+ YAG++ S++ + +    +QRKGP++V+ F+PL+++IV+I+S+ +L Q ++ G VI
Sbjct: 255 LLASAYAGIMSSSIAYYVQGLMMQRKGPVFVTAFNPLIVVIVSIMSFFVLGQGIYLGGVI 314

Query: 312 GSVLIITGLYAVLWGK 328
           G V+++ G+YAVLWGK
Sbjct: 315 GVVVLMVGVYAVLWGK 320

BLAST of Cucsa.090430 vs. TAIR10
Match: AT1G01070.1 (AT1G01070.1 nodulin MtN21 /EamA-like transporter family protein)

HSP 1 Score: 246.1 bits (627), Expect = 3.0e-65
Identity = 126/323 (39.01%), Postives = 192/323 (59.44%), Query Frame = 1

Query: 9   LIGMIILQICYAGINIISKLAMQSGMNPLVLLTYRQIFGTLAIAPFAFLTERKTRPKITF 68
           +I M++  +    +N + K A+  G+N +V+  YR     L + PFA++ ERKTRP+ITF
Sbjct: 17  VIVMVMSNVAMGSVNALVKKALDVGVNHMVIGAYRMAISALILVPFAYVLERKTRPQITF 76

Query: 69  AVLFQIFVCSLSGATGNQIFFFVGLKYTNPTISSAMANVLPAATFILAVLFRQESVRI-K 128
            ++   FV  L GA+  Q FF +GL YT+ T+S A+ ++LPA TF LA++FR E+V+I K
Sbjct: 77  RLMVDHFVSGLLGASLMQFFFLLGLSYTSATVSCALVSMLPAITFALALIFRTENVKILK 136

Query: 129 TKSGFAKVIGTIVCVSGAMLLSFYHGHTIDLGESRIHWSYVERLIKETTPTNGQGKHVLG 188
           TK+G  KVIGT++C+SGA+ L+FY G  I    S  H         +    N     +LG
Sbjct: 137 TKAGMLKVIGTLICISGALFLTFYKGPQISNSHSHSHGGASHNNNDQDKANNW----LLG 196

Query: 189 SILLLLSSFSWALWFVIQARLSVKFKAPYTSTALLCFMAFFQCGLIAVISEHNIAAWSLK 248
            + L + +   +LW + Q  LS+K+   Y+ST L+   A FQC L+++    ++  W + 
Sbjct: 197 CLYLTIGTVLLSLWMLFQGTLSIKYPCKYSSTCLMSIFAAFQCALLSLYKSRDVNDWIID 256

Query: 249 SSIRLVAALYAGVVCSALTFSITSWTIQRKGPLYVSIFSPLLLIIVAIISWALLHQQLHA 308
               +   +YAGVV  A+T   T+W I++ G ++ S F PL LI   +  + +LH  L+ 
Sbjct: 257 DRFVITVIIYAGVVGQAMTTVATTWGIKKLGAVFASAFFPLTLISATLFDFLILHTPLYL 316

Query: 309 GTVIGSVLIITGLYAVLWGKSKE 331
           G+VIGS++ ITGLY  LWGK+KE
Sbjct: 317 GSVIGSLVTITGLYMFLWGKNKE 335

BLAST of Cucsa.090430 vs. NCBI nr
Match: gi|449454123|ref|XP_004144805.1| (PREDICTED: WAT1-related protein At1g09380 [Cucumis sativus])

HSP 1 Score: 628.2 bits (1619), Expect = 7.9e-177
Identity = 321/321 (100.00%), Postives = 321/321 (100.00%), Query Frame = 1

Query: 12  MIILQICYAGINIISKLAMQSGMNPLVLLTYRQIFGTLAIAPFAFLTERKTRPKITFAVL 71
           MIILQICYAGINIISKLAMQSGMNPLVLLTYRQIFGTLAIAPFAFLTERKTRPKITFAVL
Sbjct: 1   MIILQICYAGINIISKLAMQSGMNPLVLLTYRQIFGTLAIAPFAFLTERKTRPKITFAVL 60

Query: 72  FQIFVCSLSGATGNQIFFFVGLKYTNPTISSAMANVLPAATFILAVLFRQESVRIKTKSG 131
           FQIFVCSLSGATGNQIFFFVGLKYTNPTISSAMANVLPAATFILAVLFRQESVRIKTKSG
Sbjct: 61  FQIFVCSLSGATGNQIFFFVGLKYTNPTISSAMANVLPAATFILAVLFRQESVRIKTKSG 120

Query: 132 FAKVIGTIVCVSGAMLLSFYHGHTIDLGESRIHWSYVERLIKETTPTNGQGKHVLGSILL 191
           FAKVIGTIVCVSGAMLLSFYHGHTIDLGESRIHWSYVERLIKETTPTNGQGKHVLGSILL
Sbjct: 121 FAKVIGTIVCVSGAMLLSFYHGHTIDLGESRIHWSYVERLIKETTPTNGQGKHVLGSILL 180

Query: 192 LLSSFSWALWFVIQARLSVKFKAPYTSTALLCFMAFFQCGLIAVISEHNIAAWSLKSSIR 251
           LLSSFSWALWFVIQARLSVKFKAPYTSTALLCFMAFFQCGLIAVISEHNIAAWSLKSSIR
Sbjct: 181 LLSSFSWALWFVIQARLSVKFKAPYTSTALLCFMAFFQCGLIAVISEHNIAAWSLKSSIR 240

Query: 252 LVAALYAGVVCSALTFSITSWTIQRKGPLYVSIFSPLLLIIVAIISWALLHQQLHAGTVI 311
           LVAALYAGVVCSALTFSITSWTIQRKGPLYVSIFSPLLLIIVAIISWALLHQQLHAGTVI
Sbjct: 241 LVAALYAGVVCSALTFSITSWTIQRKGPLYVSIFSPLLLIIVAIISWALLHQQLHAGTVI 300

Query: 312 GSVLIITGLYAVLWGKSKEMK 333
           GSVLIITGLYAVLWGKSKEMK
Sbjct: 301 GSVLIITGLYAVLWGKSKEMK 321

BLAST of Cucsa.090430 vs. NCBI nr
Match: gi|659070066|ref|XP_008453225.1| (PREDICTED: WAT1-related protein At1g09380 [Cucumis melo])

HSP 1 Score: 610.5 bits (1573), Expect = 1.7e-171
Identity = 309/321 (96.26%), Postives = 315/321 (98.13%), Query Frame = 1

Query: 12  MIILQICYAGINIISKLAMQSGMNPLVLLTYRQIFGTLAIAPFAFLTERKTRPKITFAVL 71
           MIILQICYAGINIISKLAMQSGMNPLVLLTYRQIFGTLAIAPFAF TERKTRPKITF VL
Sbjct: 1   MIILQICYAGINIISKLAMQSGMNPLVLLTYRQIFGTLAIAPFAFFTERKTRPKITFTVL 60

Query: 72  FQIFVCSLSGATGNQIFFFVGLKYTNPTISSAMANVLPAATFILAVLFRQESVRIKTKSG 131
           FQIFVCSLSGATGNQIFFFVGLKYTNPTISSAMANVLPAATFILAVLFRQESVRIKTKSG
Sbjct: 61  FQIFVCSLSGATGNQIFFFVGLKYTNPTISSAMANVLPAATFILAVLFRQESVRIKTKSG 120

Query: 132 FAKVIGTIVCVSGAMLLSFYHGHTIDLGESRIHWSYVERLIKETTPTNGQGKHVLGSILL 191
           FAKVIGTIVCVSGAMLLSFYHGH I+LGES+IHWSYVERLIKETTPTNGQGKHVLGSILL
Sbjct: 121 FAKVIGTIVCVSGAMLLSFYHGHIINLGESKIHWSYVERLIKETTPTNGQGKHVLGSILL 180

Query: 192 LLSSFSWALWFVIQARLSVKFKAPYTSTALLCFMAFFQCGLIAVISEHNIAAWSLKSSIR 251
           LLSSF+WALWFVIQARLSVKFKAPYTSTALLCFMAFFQCGLIAVISEHNIAAWSLKS+IR
Sbjct: 181 LLSSFAWALWFVIQARLSVKFKAPYTSTALLCFMAFFQCGLIAVISEHNIAAWSLKSTIR 240

Query: 252 LVAALYAGVVCSALTFSITSWTIQRKGPLYVSIFSPLLLIIVAIISWALLHQQLHAGTVI 311
           L+AALYAGVVCSALTFSITSW IQRKGPLYVSIFSPLLLIIVAI+SWALLHQQLH GTVI
Sbjct: 241 LIAALYAGVVCSALTFSITSWAIQRKGPLYVSIFSPLLLIIVAILSWALLHQQLHVGTVI 300

Query: 312 GSVLIITGLYAVLWGKSKEMK 333
           GSVLII GLYAVLWGKSKEMK
Sbjct: 301 GSVLIIIGLYAVLWGKSKEMK 321

BLAST of Cucsa.090430 vs. NCBI nr
Match: gi|659127231|ref|XP_008463595.1| (PREDICTED: WAT1-related protein At1g09380-like [Cucumis melo])

HSP 1 Score: 432.2 bits (1110), Expect = 8.3e-118
Identity = 214/322 (66.46%), Postives = 262/322 (81.37%), Query Frame = 1

Query: 12  MIILQICYAGINIISKLAMQSGMNPLVLLTYRQIFGTLAIAPFAFLTERKTRPKITFAVL 71
           M+++Q  YAG+NI SKLAM+ GMNPLVL+ YRQ+F T+AIAP A+  ERK RPKIT  +L
Sbjct: 14  MLVVQFLYAGLNITSKLAMEFGMNPLVLVAYRQMFATIAIAPCAYWFERKGRPKITKPIL 73

Query: 72  FQIFVCSLSGATGNQIFFFVGLKYTNPTISSAMANVLPAATFILAVLFRQESVRIKTKSG 131
           FQI +CSL+GATGNQ+F++VGLKY+ PTI+ A+ NVLPAATF+LAVLFRQESVRIKT  G
Sbjct: 74  FQILLCSLTGATGNQVFYYVGLKYSTPTIACALTNVLPAATFVLAVLFRQESVRIKTSPG 133

Query: 132 FAKVIGTIVCVSGAMLLSFYHGHTIDLGESRIHWSYVERLIKETTPTNGQGKHVLGSILL 191
            AKVIGT+VCV GAMLLSFY G TI+LGES IHW Y   L++  + +N QG  + GS+ L
Sbjct: 134 AAKVIGTVVCVGGAMLLSFYRGQTIELGESGIHWKYA-GLMRGGSSSN-QGSSIWGSLCL 193

Query: 192 LLSSFSWALWFVIQARLSVKFKAPYTSTALLCFMAFFQCGLIAVISEH-NIAAWSLKSSI 251
           ++SS +WA WFVIQAR++ KF APYTSTAL+ FMA  QCG IAV  EH  +AAWSLKSSI
Sbjct: 194 IISSVAWAAWFVIQARVNEKFPAPYTSTALMTFMATIQCGAIAVGIEHKTLAAWSLKSSI 253

Query: 252 RLVAALYAGVVCSALTFSITSWTIQRKGPLYVSIFSPLLLIIVAIISWALLHQQLHAGTV 311
           RLV ALYAGV CS + F +TSW+IQ+KGPLY S+FSP LL+IVAI SWA   ++L+ GTV
Sbjct: 254 RLVGALYAGVACSGMAFCLTSWSIQKKGPLYASVFSPFLLVIVAIFSWAFFQEKLYVGTV 313

Query: 312 IGSVLIITGLYAVLWGKSKEMK 333
           +GS+LI+ GLYAVLWGK+KE+K
Sbjct: 314 VGSLLIVVGLYAVLWGKTKEVK 333

BLAST of Cucsa.090430 vs. NCBI nr
Match: gi|743890239|ref|XP_011038989.1| (PREDICTED: WAT1-related protein At1g09380 [Populus euphratica])

HSP 1 Score: 429.1 bits (1102), Expect = 7.0e-117
Identity = 201/332 (60.54%), Postives = 262/332 (78.92%), Query Frame = 1

Query: 1   MEGDDITALIGMIILQICYAGINIISKLAMQSGMNPLVLLTYRQIFGTLAIAPFAFLTER 60
           M+  D+   + M I+Q  YAG+NI SKLAM SGM PLVL+ YRQIF T+A+ PFA+  E 
Sbjct: 1   MDMADVLPFLAMAIVQFGYAGMNITSKLAMDSGMKPLVLVGYRQIFATIAMVPFAYFFEW 60

Query: 61  KTRPKITFAVLFQIFVCSLSGATGNQIFFFVGLKYTNPTISSAMANVLPAATFILAVLFR 120
           +TRPKIT ++L QIF+CSL+G TGNQ+F+F+GL+ + PTI  A+ N+LPA TFILAVLFR
Sbjct: 61  RTRPKITMSLLLQIFICSLTGVTGNQVFYFIGLENSTPTIGCALTNILPAVTFILAVLFR 120

Query: 121 QESVRIKTKSGFAKVIGTIVCVSGAMLLSFYHGHTIDLGESRIHWSYVERLIKETTPTNG 180
           QESV IK  SG AK++GTIVCV GAMLLSFYHGH I++GES IHW+Y +     T  TN 
Sbjct: 121 QESVGIKKASGQAKLLGTIVCVGGAMLLSFYHGHMINIGESSIHWNYADSTGNST--TNK 180

Query: 181 QGKHVLGSILLLLSSFSWALWFVIQARLSVKFKAPYTSTALLCFMAFFQCGLIAVISEHN 240
           +   VLGS+ ++ S+ SWA+WF +QA++S+KF APYT T L+CFM   +C +I + + H 
Sbjct: 181 KSNFVLGSLCIIASAISWAIWFTVQAKVSLKFPAPYTCTLLMCFMGSIECVVIGIGANHK 240

Query: 241 IAAWSLKSSIRLVAALYAGVVCSALTFSITSWTIQRKGPLYVSIFSPLLLIIVAIISWAL 300
           ++ WSL+S  RL+AALYAG+VCSAL FS+TSW+IQRKG LYVS+FSPLLL+IVA++SWAL
Sbjct: 241 VSEWSLRSPGRLIAALYAGIVCSALAFSLTSWSIQRKGALYVSVFSPLLLVIVAVLSWAL 300

Query: 301 LHQQLHAGTVIGSVLIITGLYAVLWGKSKEMK 333
           LH++++ GT +GS+LI+ GLYAVLWGK KE+K
Sbjct: 301 LHEKIYVGTAVGSILIVAGLYAVLWGKDKELK 330

BLAST of Cucsa.090430 vs. NCBI nr
Match: gi|449443520|ref|XP_004139525.1| (PREDICTED: WAT1-related protein At1g09380-like [Cucumis sativus])

HSP 1 Score: 429.1 bits (1102), Expect = 7.0e-117
Identity = 212/331 (64.05%), Postives = 265/331 (80.06%), Query Frame = 1

Query: 3   GDDITALIGMIILQICYAGINIISKLAMQSGMNPLVLLTYRQIFGTLAIAPFAFLTERKT 62
           GD +  L+ M+++Q  YAG+NI SKLAM+ GMNPLVL+ YRQ+F T+AIAP A+  ERK 
Sbjct: 5   GDFLPPLV-MLVVQFLYAGLNITSKLAMEFGMNPLVLVAYRQMFATIAIAPCAYWFERKG 64

Query: 63  RPKITFAVLFQIFVCSLSGATGNQIFFFVGLKYTNPTISSAMANVLPAATFILAVLFRQE 122
           RPKIT  +LFQI +CSL+GAT NQ+F++VGLKY+ PTI+ A+ NVLPAATF+LAVLFRQE
Sbjct: 65  RPKITKPILFQILLCSLTGATANQVFYYVGLKYSTPTIACALTNVLPAATFVLAVLFRQE 124

Query: 123 SVRIKTKSGFAKVIGTIVCVSGAMLLSFYHGHTIDLGESRIHWSYVERLIKETTPTNGQG 182
           SV IKT  G AKVIGT+VCV GAMLLSFY G TI+LG+S IHW Y E +  E+  ++ QG
Sbjct: 125 SVGIKTSPGAAKVIGTVVCVGGAMLLSFYRGQTIELGKSGIHWKYAELMRGES--SSNQG 184

Query: 183 KHVLGSILLLLSSFSWALWFVIQARLSVKFKAPYTSTALLCFMAFFQCGLIAVISEH-NI 242
             + GS+ L++SS +WA WFVIQAR++ KF APYTSTAL+ FMA  QCG IAV  EH  +
Sbjct: 185 SSIWGSLCLIISSVAWAAWFVIQARVNEKFPAPYTSTALMTFMATIQCGAIAVGVEHKTL 244

Query: 243 AAWSLKSSIRLVAALYAGVVCSALTFSITSWTIQRKGPLYVSIFSPLLLIIVAIISWALL 302
           AAWSLKSSIRLV ALYAGV CS + F +TSW+IQ++GPLY S+FSP LL+IVAI SWA  
Sbjct: 245 AAWSLKSSIRLVGALYAGVACSGMAFCLTSWSIQKRGPLYASVFSPFLLVIVAIFSWAFF 304

Query: 303 HQQLHAGTVIGSVLIITGLYAVLWGKSKEMK 333
            ++L+ GTV+GS+LI+ GLY+VLWGK+KE+K
Sbjct: 305 QEKLYVGTVVGSLLIVVGLYSVLWGKTKEVK 332

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
WTR2_ARATH9.5e-11457.70WAT1-related protein At1g09380 OS=Arabidopsis thaliana GN=At1g09380 PE=2 SV=1[more]
WTR38_ARATH1.4e-6942.55WAT1-related protein At5g07050 OS=Arabidopsis thaliana GN=At5g07050 PE=2 SV=1[more]
WTR24_ARATH1.8e-6738.70WAT1-related protein At3g30340 OS=Arabidopsis thaliana GN=At3g30340 PE=2 SV=1[more]
WTR15_ARATH1.1e-6440.19WAT1-related protein At2g40900 OS=Arabidopsis thaliana GN=At2g40900 PE=2 SV=1[more]
WTR1_ARATH5.3e-6439.01WAT1-related protein At1g01070 OS=Arabidopsis thaliana GN=At1g01070 PE=2 SV=1[more]
Match NameE-valueIdentityDescription
A0A0A0LW49_CUCSA4.9e-11764.05WAT1-related protein OS=Cucumis sativus GN=Csa_1G181350 PE=3 SV=1[more]
M5W6P9_PRUPE1.9e-11660.74WAT1-related protein (Fragment) OS=Prunus persica GN=PRUPE_ppa018179mg PE=3 SV=1[more]
W9R2P6_9ROSA5.4e-11660.00WAT1-related protein OS=Morus notabilis GN=L484_018875 PE=3 SV=1[more]
A0A0A0LGK0_CUCSA7.1e-11689.16WAT1-related protein OS=Cucumis sativus GN=Csa_2G034730 PE=3 SV=1[more]
A0A061F4Z0_THECC2.7e-11560.67WAT1-related protein OS=Theobroma cacao GN=TCM_026803 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
AT1G09380.15.4e-11557.70 nodulin MtN21 /EamA-like transporter family protein[more]
AT5G07050.18.1e-7142.55 nodulin MtN21 /EamA-like transporter family protein[more]
AT3G30340.19.9e-6938.70 nodulin MtN21 /EamA-like transporter family protein[more]
AT2G40900.16.0e-6640.19 nodulin MtN21 /EamA-like transporter family protein[more]
AT1G01070.13.0e-6539.01 nodulin MtN21 /EamA-like transporter family protein[more]
Match NameE-valueIdentityDescription
gi|449454123|ref|XP_004144805.1|7.9e-177100.00PREDICTED: WAT1-related protein At1g09380 [Cucumis sativus][more]
gi|659070066|ref|XP_008453225.1|1.7e-17196.26PREDICTED: WAT1-related protein At1g09380 [Cucumis melo][more]
gi|659127231|ref|XP_008463595.1|8.3e-11866.46PREDICTED: WAT1-related protein At1g09380-like [Cucumis melo][more]
gi|743890239|ref|XP_011038989.1|7.0e-11760.54PREDICTED: WAT1-related protein At1g09380 [Populus euphratica][more]
gi|449443520|ref|XP_004139525.1|7.0e-11764.05PREDICTED: WAT1-related protein At1g09380-like [Cucumis sativus][more]
The following terms have been associated with this gene:
Vocabulary: INTERPRO
TermDefinition
IPR000620EamA_dom
Vocabulary: Cellular Component
TermDefinition
GO:0016021integral component of membrane
GO:0016020membrane
Vocabulary: Molecular Function
TermDefinition
GO:0022857transmembrane transporter activity
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0055085 transmembrane transport
biological_process GO:0008150 biological_process
cellular_component GO:0016021 integral component of membrane
cellular_component GO:0016020 membrane
molecular_function GO:0022857 transmembrane transporter activity

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Cucsa.090430.1Cucsa.090430.1mRNA


Analysis Name: InterPro Annotations of cucumber (Gy14)
Date Performed: 2017-01-17
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR000620EamA domainPFAMPF00892EamAcoord: 9..148
score: 1.1E-14coord: 186..324
score: 3.4
NoneNo IPR availablePANTHERPTHR31218:SF11SUBFAMILY NOT NAMEDcoord: 3..332
score: 3.8E
NoneNo IPR availableunknownSSF103481Multidrug resistance efflux transporter EmrEcoord: 248..329
score: 1.11E-8coord: 42..150
score: 3.