Cucsa.088400 (gene) Cucumber (Gy14) v1

BLAST of Cucsa.088400 vs. Swiss-Prot
Match: PUS3_ARATH (RNA pseudouridine synthase 3, mitochondrial OS=Arabidopsis thaliana GN=At1g78910 PE=2 SV=1)

HSP 1 Score: 655.2 bits (1689), Expect = 5.4e-187
Identity = 321/473 (67.86%), Postives = 389/473 (82.24%), Query Frame = 1

Query: 8   HLLLATRCYSRISPPPPAYAKPIIRVSNNIAQLGSPKEGPKARQLLSLPPFPGYPLPGKN 67
           +L +  R YSR++PPP +    +IRV+NN+A LG PK+GP  RQL+SLPPFPG+PLPGKN
Sbjct: 12  YLTVLIRRYSRVAPPPSS----VIRVTNNVAHLGPPKQGPLPRQLISLPPFPGHPLPGKN 71

Query: 68  QVGVSGEST----HVTAIRWVKYYFDEIQDSQIQSHFRKGLVQVECP--QLGGLDEKGNL 127
             G  G+      HVTAI WVKYYF+EI D  IQ+HF KGLVQ+E    +    +++   
Sbjct: 72  -AGADGDDGDSGGHVTAISWVKYYFEEIYDKAIQTHFTKGLVQMEFRGRRDASREKEDGA 131

Query: 128 KPMRKIRSGEIMETGARIHLPVSVAEMKVARRFDTIPSGTLYPNADEIKYLQRLVKYKDS 187
            PMRKI+  E+M+ G +I LPVS+AEM++++R+DTIPSGTLYPNADEI YLQRLV++KDS
Sbjct: 132 IPMRKIKHNEVMQIGDKIWLPVSIAEMRISKRYDTIPSGTLYPNADEIAYLQRLVRFKDS 191

Query: 188 AIIVLNKPPKLPVKGTLPVHNSMDALADAALSYDYDDGPKLVHRLDRESSGILLMGRTKE 247
           AIIVLNKPPKLPVKG +P+HNSMDALA AALS+  D+GP+LVHRLDRE+SG+L+MGRTKE
Sbjct: 192 AIIVLNKPPKLPVKGNVPIHNSMDALAAAALSFGNDEGPRLVHRLDRETSGLLVMGRTKE 251

Query: 248 SVDHLQWLFSNIRNAKFTCKAWEEACNATYQKYWALVIGCPREREGVISAPLSKVLLDDG 307
           S+D+L  +FS+ +    +CKAW +AC A YQ+YWALVIG P+E+EG+ISAPLSKVLLDDG
Sbjct: 252 SIDYLHSVFSDYKGRNSSCKAWNKACEAMYQQYWALVIGSPKEKEGLISAPLSKVLLDDG 311

Query: 308 KTERVILANKASIEASQEAITEYRVLGPTINGCSWIELCPRTSRKHQLRIHCAEALGTPI 367
           KT+RV+LA  +  EASQ+AITEY+VLGP INGCSW+EL P TSRKHQLR+HCAEALGTPI
Sbjct: 312 KTDRVVLAQGSGFEASQDAITEYKVLGPKINGCSWVELRPITSRKHQLRVHCAEALGTPI 371

Query: 368 VGDYKYGWFVHRRWKQMPRVDVEPISGRPYKLRRPEGLDVQKGSVLSKVPLLHLHCRELV 427
           VGDYKYGWFVH+RWKQMP+VD+EP +G+PYKLRRPEGLDVQKGSVLSKVPLLHLHCRE+V
Sbjct: 372 VGDYKYGWFVHKRWKQMPQVDIEPTTGKPYKLRRPEGLDVQKGSVLSKVPLLHLHCREMV 431

Query: 428 LPNIAKFLNVFYGKGPNRPVSS---FDSDLLRFVARMPSHMRISWNLMSSYLV 472
           LPNIAKFL+V   +    P+ +      DLLRFVA MPSHM+ISWNLMSSYLV
Sbjct: 432 LPNIAKFLHVM-NQQETEPLHTGIIDKPDLLRFVASMPSHMKISWNLMSSYLV 478

BLAST of Cucsa.088400 vs. Swiss-Prot
Match: PUS3_ORYSJ (RNA pseudouridine synthase 3, mitochondrial OS=Oryza sativa subsp. japonica GN=Os08g0520100 PE=2 SV=1)

HSP 1 Score: 586.6 bits (1511), Expect = 2.3e-166
Identity = 294/475 (61.89%), Postives = 362/475 (76.21%), Query Frame = 1

Query: 6   RRHLLLATRCYSRISPPPPAYAKPII-RVS-NNIAQLGSPKEGPKARQLLSLPPFPGY-- 65
           RR +  A R  SR++PP PA A P++ RV  +N+A+LG PK GP+ RQLLSLPPFPG   
Sbjct: 5   RRRVGAAVRWLSRLAPPAPAEADPVVVRVDGSNVARLGKPKPGPRPRQLLSLPPFPGGGD 64

Query: 66  --PLPGKNQVGVSGESTHVTAIRWVKYYFDEIQDSQIQSHFRKGLVQVECP--QLGGLDE 125
             PLPG+     +     VTA+ WVK+Y  ++    +Q+HF K LV  EC   ++     
Sbjct: 65  GDPLPGRK----AAAPRRVTAVSWVKHYLADVPQEVVQAHFNKRLVYSECSDHEVSVETI 124

Query: 126 KGNLKPMRKIRSGEIMETGARIHLPVSVAEMKVARRFDTIPSGTLYPNADEIKYLQRLVK 185
           K     ++KI+  ++ME G RIHLPVSVAE ++ +R++TIP+ TL+PN DEI+YL+RLV 
Sbjct: 125 KSQKHHLKKIKHNDVMEPGMRIHLPVSVAEGEIKKRYETIPTATLHPNKDEIEYLRRLVI 184

Query: 186 YKDSAIIVLNKPPKLPVKGTLPVHNSMDALADAALSYDYDDGPKLVHRLDRESSGILLMG 245
           +KDSAI+VLNKPPK+P+KG LPVHNSMD LA AALSY  ++GPKLVHRLDRESSG+LL G
Sbjct: 185 HKDSAILVLNKPPKVPMKGNLPVHNSMDVLAAAALSYGNEEGPKLVHRLDRESSGLLLFG 244

Query: 246 RTKESVDHLQWLFSNIRNAKFTCKAWEEACNATYQKYWALVIGCPREREGVISAPLSKVL 305
           RTKES   L WLF+++  AK   + W  AC A  Q+YWALVIG P+EREG+ISAPLSKVL
Sbjct: 245 RTKESFTRLHWLFTSVNLAKTNSQVWNAACEAYMQRYWALVIGTPKEREGIISAPLSKVL 304

Query: 306 LDDGKTERVILANKASIEASQEAITEYRVLGPTINGCSWIELCPRTSRKHQLRIHCAEAL 365
           LDDGK ERVILA+ + I+ +QEA+T YRV+GPTI+GCSWIEL P T RKHQLR+HCAEAL
Sbjct: 305 LDDGKAERVILAHPSGIDGAQEAVTAYRVMGPTIHGCSWIELRPLTGRKHQLRVHCAEAL 364

Query: 366 GTPIVGDYKYGWFVHRRWKQMPRVDVEPISGRPYKLRRPEGLDVQKGSVLSKVPLLHLHC 425
           GTPIVGDYKYGWFVH+RWKQ P+ D EP +G PYKLRRPEGL++QKGSVLSKVPLLHLHC
Sbjct: 365 GTPIVGDYKYGWFVHQRWKQNPQPDFEPFTGEPYKLRRPEGLEIQKGSVLSKVPLLHLHC 424

Query: 426 RELVLPNIAKFLNVFYGKGPN-RPVSSFDSDLLRFVARMPSHMRISWNLMSSYLV 472
           RE+V+PNIAKFL+       N  P S    +LLRF+A MP+HM+ISWN+MSSYLV
Sbjct: 425 REMVIPNIAKFLSSNGEWHENGAPWSKEKPNLLRFIAPMPAHMKISWNIMSSYLV 475

BLAST of Cucsa.088400 vs. Swiss-Prot
Match: PUS4_ARATH (RNA pseudouridine synthase 4, mitochondrial OS=Arabidopsis thaliana GN=At3g19440 PE=2 SV=1)

HSP 1 Score: 260.0 bits (663), Expect = 5.0e-68
Identity = 160/425 (37.65%), Postives = 241/425 (56.71%), Query Frame = 1

Query: 53  LSLPPFPGYP---------LPGKNQVGVSGESTHVTAIRWVKYYFDEIQDSQIQSHFRKG 112
           L+LPPF             L   + V  S +++  TA+RW+     ++  + +Q  FR  
Sbjct: 56  LTLPPFSPTIDGTAVGKDLLSDGDSVKSSTDNSKTTALRWILRCRPDLPRTLVQKLFRLR 115

Query: 113 LVQVECPQLGGLDEKGNLKPMRKIRSGEIMETGARIHLPVSVAEMKVARRFDTIPSGTLY 172
            V+ E       DE    + ++++ + E +  G RI+LP+SV       +       +  
Sbjct: 116 QVRREMSMSVDGDELQRSQ-LKRVAAKESLNVGDRIYLPLSVDND--TPQTPPAKKESFQ 175

Query: 173 PNADEIKYLQRLVKYKDSAIIVLNKPPKLPVKGTLPVHNSMDALADAALSYDYDDGPKLV 232
            + +E K++  LV YKD AIIVLNKP  L V+G   +  S+D LA + L +D  + P+LV
Sbjct: 176 CSDEERKFVCSLVLYKDPAIIVLNKPHGLAVQGGSGIKTSIDELAASCLKFDKSESPRLV 235

Query: 233 HRLDRESSGILLMGRTKESVDHLQWLFSNIRNAKFTCKAWEEACNATYQKYWALVIGCPR 292
           HRLDR+ SG+L++ RT+ +   L  +F   +    +    ++   +  +KY ALVIGCP 
Sbjct: 236 HRLDRDCSGLLVLARTQTAATVLHSIFRE-KTTGASAYGVKKNVKSLKRKYMALVIGCPP 295

Query: 293 EREGVISAPLSKVLLDDGKTERVILANKASIEASQEAITEYRVLGPTINGCSWIELCPRT 352
            + G ISAPL KV++DDGK+ER+ + +   + ++Q AITEYRV+  + +G +W+EL P T
Sbjct: 296 RQRGQISAPLRKVVVDDGKSERITVNDNGELVSTQHAITEYRVIESSPHGYTWLELRPLT 355

Query: 353 SRKHQLRIHCAEALGTPIVGDYKYGWFVHRRWKQMPRVDVEPISGRPYKLRRPEGLDVQK 412
            RKHQLR+HCAE LGTPIVGDYKYGW  H+  +     +  P      +   P GLD+  
Sbjct: 356 GRKHQLRVHCAEVLGTPIVGDYKYGWQAHKAREPFVSSENNPTK----QSSSPFGLDLDG 415

Query: 413 GSVLSKVPLLHLHCRELVLPNIAKFLNVFYGKGPNRPVSSFDSDLLRFVARMPSHMRISW 469
           G V SK P LHLH +++ LPNI++ L        +  +S  DS  L+F A +PSHM++S+
Sbjct: 416 GDVSSKQPHLHLHSKQIDLPNISQLLEKMQ-VSSDSDISDLDS--LKFDAPLPSHMQLSF 469

BLAST of Cucsa.088400 vs. Swiss-Prot
Match: PUS4_ORYSJ (RNA pseudouridine synthase 4, mitochondrial OS=Oryza sativa subsp. japonica GN=Os09g0103500 PE=2 SV=1)

HSP 1 Score: 174.5 bits (441), Expect = 2.8e-42
Identity = 111/305 (36.39%), Postives = 168/305 (55.08%), Query Frame = 1

Query: 53  LSLPPFPGYPLPGKNQV-----GVSGESTH-VTAIRWVKYYFDEIQDSQIQSHFRKGLVQ 112
           L LPPF         +      G  G+     TAI+WV+    ++  S +Q  FR   V+
Sbjct: 53  LQLPPFAPLDAAAAARAISRGGGEGGDGEQGATAIKWVRRCCPDLPTSLVQKLFRLRKVK 112

Query: 113 --VECPQLGGLDEKGNLKPMRKIRSGEIMETGARIHLPVSVAEMKVARRFDTIPSGTLYP 172
             V   ++   D       +R++ + + +  G  + LPV++ E  VA +         + 
Sbjct: 113 KNVVTAEISSADASAEQHRLRRVSAKDQLMPGDILFLPVNLKESSVAEKTKK------FD 172

Query: 173 NADEIKYLQRLVKYKDSAIIVLNKPPKLPVKGTLPVHNSMDALADAALSYDYDDGPKLVH 232
           N +EI +L+ L  YKD AIIV+NKPP +PV+G + + NS+D LA +    +  + P+LVH
Sbjct: 173 NRNEINFLRGLEIYKDEAIIVVNKPPGMPVQGGVGIKNSIDVLA-SMFEENSSEAPRLVH 232

Query: 233 RLDRESSGILLMGRTKESVDHLQWLFSNIRNAKFTCKAWEEACNATYQ-KYWALVIGCPR 292
           RLDR+ SGIL++GR + S   L  +F      + T  A  +      Q KY ALVIG PR
Sbjct: 233 RLDRDCSGILVLGRNQLSTSMLHAIFR-----EKTADALADGTQHVLQRKYVALVIGTPR 292

Query: 293 EREGVISAPLSKVLLDDGKTERVILANKASIEASQEAITEYRVLGPTINGCSWIELCPRT 349
             +G++SAPL+K+LL DGK+ER+ +   ++  + Q+A+TEYRV+     G +W+EL PRT
Sbjct: 293 HPKGLLSAPLAKILLQDGKSERLTIRASSNAASVQDALTEYRVIESCPQGYTWLELFPRT 345

BLAST of Cucsa.088400 vs. Swiss-Prot
Match: RUSD4_XENTR (RNA pseudouridylate synthase domain-containing protein 4 OS=Xenopus tropicalis GN=rpusd4 PE=2 SV=1)

HSP 1 Score: 116.7 bits (291), Expect = 6.9e-25
Identity = 101/313 (32.27%), Postives = 145/313 (46.33%), Query Frame = 1

Query: 136 GARIHLPVSVAEMKVARRFDTIPSGTLYPNADEIKYLQRL---VKYKDSAIIVLNKPPKL 195
           GAR+      AE KV    +     T   NA  I YL+ L   V  +D  ++++NKP  L
Sbjct: 11  GARMLAERIRAERKV---LEGAARETSRKNAPTIVYLRELKSQVVREDPELVLVNKPHGL 70

Query: 196 PVKGTLPVHNSMDALADAALSYDYD---DGPKLVHRLDRESSGILLMGRTKESVDHLQWL 255
           PV G   V  S+ +L  A   + +    +  KL HRLDR+++G L++ RT E+ + +Q  
Sbjct: 71  PVHGGPTVERSVASLLPALAKHHFGWKAEPLKLCHRLDRDTTGALILARTTEAAERVQ-- 130

Query: 256 FSNIRNAKFTCKAWEEACNATYQKYWALVIGCPREREGVISAPLSKV------------- 315
                      +A  E     ++ YWAL +G P  REG++  P+ +              
Sbjct: 131 -----------QALRE--REVHRVYWALCLGTPSPREGILDIPIMEKETSGPQKHYKMAL 190

Query: 316 -----LLDDGKTERVILANKASIEASQEAITEYRVLGPTINGCSWIELCPRTSRKHQLRI 375
                + ++G  ERV +   A      EA+T YR LG   +G S +EL P T  KHQLR+
Sbjct: 191 SPRFRVSEEGAVERVRVPRSA-----HEAVTRYRTLGAA-SGASLVELHPITGVKHQLRV 250

Query: 376 HCAEALGTPIVGDYKYGWFVHRRWKQMPRVDVEPISGRPYKLRRPEGLDVQKGSVLSKVP 425
           H A  L  PI+GD+KY  +  R   Q P          P  + R  GL V +   LS   
Sbjct: 251 HLALGLNCPILGDHKYSHW-GRLAPQKP----------PDSVLRALGLTVPQARTLS--- 284

BLAST of Cucsa.088400 vs. TrEMBL
Match: A0A0A0LQ04_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_2G402020 PE=4 SV=1)

HSP 1 Score: 962.6 bits (2487), Expect = 1.8e-277
Identity = 470/471 (99.79%), Postives = 471/471 (100.00%), Query Frame = 1

Query: 1   MQRSMRRHLLLATRCYSRISPPPPAYAKPIIRVSNNIAQLGSPKEGPKARQLLSLPPFPG 60
           MQRSMRRHLLLATRCYSRISPPPPAYAKPIIRVSNNIAQLGSPKEGPKARQLLSLPPFPG
Sbjct: 73  MQRSMRRHLLLATRCYSRISPPPPAYAKPIIRVSNNIAQLGSPKEGPKARQLLSLPPFPG 132

Query: 61  YPLPGKNQVGVSGESTHVTAIRWVKYYFDEIQDSQIQSHFRKGLVQVECPQLGGLDEKGN 120
           YPLPGKNQVGVSGESTHVTAIRWVKYYFDEIQDSQIQSHFRKGLVQVECPQLGGLDEKGN
Sbjct: 133 YPLPGKNQVGVSGESTHVTAIRWVKYYFDEIQDSQIQSHFRKGLVQVECPQLGGLDEKGN 192

Query: 121 LKPMRKIRSGEIMETGARIHLPVSVAEMKVARRFDTIPSGTLYPNADEIKYLQRLVKYKD 180
           LKPMRKIRSGEIMETGARIHLPVSVAEMKVARRFDTIPSGTLYPNADEIKYLQRLVKYKD
Sbjct: 193 LKPMRKIRSGEIMETGARIHLPVSVAEMKVARRFDTIPSGTLYPNADEIKYLQRLVKYKD 252

Query: 181 SAIIVLNKPPKLPVKGTLPVHNSMDALADAALSYDYDDGPKLVHRLDRESSGILLMGRTK 240
           SAIIVLNKPPKLPVKGTLPVHNSMDALADAALSYDYDDGPKLVHRLDRESSGILLMGRTK
Sbjct: 253 SAIIVLNKPPKLPVKGTLPVHNSMDALADAALSYDYDDGPKLVHRLDRESSGILLMGRTK 312

Query: 241 ESVDHLQWLFSNIRNAKFTCKAWEEACNATYQKYWALVIGCPREREGVISAPLSKVLLDD 300
           ESVDHLQWLFSNIRNAKFTCKAWEEACNATYQKYWALVIGCPREREGVISAPLSKVLLDD
Sbjct: 313 ESVDHLQWLFSNIRNAKFTCKAWEEACNATYQKYWALVIGCPREREGVISAPLSKVLLDD 372

Query: 301 GKTERVILANKASIEASQEAITEYRVLGPTINGCSWIELCPRTSRKHQLRIHCAEALGTP 360
           GKTERV+LANKASIEASQEAITEYRVLGPTINGCSWIELCPRTSRKHQLRIHCAEALGTP
Sbjct: 373 GKTERVVLANKASIEASQEAITEYRVLGPTINGCSWIELCPRTSRKHQLRIHCAEALGTP 432

Query: 361 IVGDYKYGWFVHRRWKQMPRVDVEPISGRPYKLRRPEGLDVQKGSVLSKVPLLHLHCREL 420
           IVGDYKYGWFVHRRWKQMPRVDVEPISGRPYKLRRPEGLDVQKGSVLSKVPLLHLHCREL
Sbjct: 433 IVGDYKYGWFVHRRWKQMPRVDVEPISGRPYKLRRPEGLDVQKGSVLSKVPLLHLHCREL 492

Query: 421 VLPNIAKFLNVFYGKGPNRPVSSFDSDLLRFVARMPSHMRISWNLMSSYLV 472
           VLPNIAKFLNVFYGKGPNRPVSSFDSDLLRFVARMPSHMRISWNLMSSYLV
Sbjct: 493 VLPNIAKFLNVFYGKGPNRPVSSFDSDLLRFVARMPSHMRISWNLMSSYLV 543

BLAST of Cucsa.088400 vs. TrEMBL
Match: B9SLG9_RICCO (Ribosomal pseudouridine synthase, putative OS=Ricinus communis GN=RCOM_0686660 PE=4 SV=1)

HSP 1 Score: 721.8 bits (1862), Expect = 5.2e-205
Identity = 348/466 (74.68%), Postives = 393/466 (84.33%), Query Frame = 1

Query: 7   RHLLLATRCYSRISPPPPAYAKPIIRVSNNIAQLGSPKEGPKARQLLSLPPFPGYPLPGK 66
           R ++   R YSRI+PPPPAYA P+IRVSNN+A LGSPK+GPK RQLLSLPPFPG PLPG+
Sbjct: 8   RRVVSQGRSYSRIAPPPPAYANPVIRVSNNVAHLGSPKDGPKPRQLLSLPPFPGLPLPGR 67

Query: 67  NQVGVSGESTHVTAIRWVKYYFDEIQDSQIQSHFRKGLVQVECPQLGGLDEKGNLKPMRK 126
           N +     ++HVTAI WVKYYFDEI DS IQSHF KGL  +ECP   G  E G    +RK
Sbjct: 68  NSM-----ASHVTAISWVKYYFDEILDSAIQSHFNKGLAHIECPNGSGSIEYGGQMSLRK 127

Query: 127 IRSGEIMETGARIHLPVSVAEMKVARRFDTIPSGTLYPNADEIKYLQRLVKYKDSAIIVL 186
           I+  +IME GAR+H+ VSVAE ++++RFDTIPSGTLYPNADEI+YLQRLVKYKDSAIIVL
Sbjct: 128 IKHNDIMEAGARVHVAVSVAETRISKRFDTIPSGTLYPNADEIQYLQRLVKYKDSAIIVL 187

Query: 187 NKPPKLPVKGTLPVHNSMDALADAALSYDYDDGPKLVHRLDRESSGILLMGRTKESVDHL 246
           NKPP+LPVKG LP+HNSMDALA AALSYDYD+GPKLV RLDRESSG+LLMGRTKES+DHL
Sbjct: 188 NKPPRLPVKGNLPLHNSMDALAAAALSYDYDEGPKLVQRLDRESSGLLLMGRTKESIDHL 247

Query: 247 QWLFSNIRNAKFTCKAWEEACNATYQKYWALVIGCPREREGVISAPLSKVLLDDGKTERV 306
           QWLFS++  +   CKAW +AC ATYQ+YWALVIG P+E+EG+I APLSKVLL+DGKTERV
Sbjct: 248 QWLFSDVNKSNSRCKAWNDACKATYQRYWALVIGSPKEKEGLIRAPLSKVLLNDGKTERV 307

Query: 307 ILANKASIEASQEAITEYRVLGPTINGCSWIELCPRTSRKHQLRIHCAEALGTPIVGDYK 366
           +LA  + +EASQEA+TEYRVLGP INGCSWIEL P TSRKHQLR+HCAEALGTPIVGDYK
Sbjct: 308 VLAQHSGLEASQEALTEYRVLGPRINGCSWIELRPLTSRKHQLRVHCAEALGTPIVGDYK 367

Query: 367 YGWFVHRRWKQMPRVDVEPISGRPYKLRRPEGLDVQKGSVLSKVPLLHLHCRELVLPNIA 426
           YGW VH+RWKQMPRVD+EP SG+PYKLRRP GLDVQKGSVLSKVPLLHLHCRE VLPNIA
Sbjct: 368 YGWCVHQRWKQMPRVDIEPFSGKPYKLRRPAGLDVQKGSVLSKVPLLHLHCREFVLPNIA 427

Query: 427 KFLNVFYGKGPN-RPVSSFDSDLLRFVARMPSHMRISWNLMSSYLV 472
           KFL V   K  N  P      DLLRFVA MP+HM+ISWNLMSSYLV
Sbjct: 428 KFLAVLNEKSENIHPALDAKPDLLRFVASMPTHMKISWNLMSSYLV 468

BLAST of Cucsa.088400 vs. TrEMBL
Match: D7SS16_VITVI (Putative uncharacterized protein OS=Vitis vinifera GN=VIT_09s0054g00780 PE=4 SV=1)

HSP 1 Score: 717.2 bits (1850), Expect = 1.3e-203
Identity = 348/470 (74.04%), Postives = 403/470 (85.74%), Query Frame = 1

Query: 9   LLLATRCYSRISPPPPAYAKPIIRVSNNIAQLGSPKEGPKARQLLSLPPFPGYPLPGKNQ 68
           L    R YSRI+PP PA A P+IRVSNN+AQLGSPKEGPK RQLLSLPPFP +PLPGK+ 
Sbjct: 12  LFPVVRHYSRIAPPRPASAAPVIRVSNNVAQLGSPKEGPKPRQLLSLPPFPAHPLPGKSL 71

Query: 69  VGVSGESTHVTAIRWVKYYFDEIQDSQIQSHFRKGLVQVECPQL--GGLDEKGNLKPMRK 128
           V VSG  THVTAI W+KYYFDEI  S +QSHF KGLV +E   L    L+++G +KP+RK
Sbjct: 72  VNVSGLPTHVTAISWIKYYFDEIPGSVVQSHFNKGLVHMERSNLDDSSLEKEGQIKPLRK 131

Query: 129 IRSGEIMETGARIHLPVSVAEMKVARRFDTIPSGTLYPNADEIKYLQRL--VKYKDSAII 188
           I+  E+ME G RI++PVSVAE ++++RFDTIPSGTL+PNADEI+YLQRL  +K++D AI+
Sbjct: 132 IKPNEVMEAGTRIYVPVSVAEARISKRFDTIPSGTLHPNADEIEYLQRLWLLKFQDPAIL 191

Query: 189 VLNKPPKLPVKGTLPVHNSMDALADAALSYDYDDGPKLVHRLDRESSGILLMGRTKESVD 248
           VLNKPPK+P+KG LPVHNSMDALA AAL++DY++GPKLVHRLDRESSG+LLMGRTKESV 
Sbjct: 192 VLNKPPKVPLKGNLPVHNSMDALAAAALTFDYEEGPKLVHRLDRESSGLLLMGRTKESVA 251

Query: 249 HLQWLFSNIRNAKFTCKAWEEACNATYQKYWALVIGCPREREGVISAPLSKVLLDDGKTE 308
           HL WLFS+I  AK   KAW++AC AT Q+YWALVIG P+++EGVI APLSKVLLDDGKTE
Sbjct: 252 HLHWLFSDINKAKSLSKAWDDACEATCQRYWALVIGSPKQKEGVIHAPLSKVLLDDGKTE 311

Query: 309 RVILANKASIEASQEAITEYRVLGPTINGCSWIELCPRTSRKHQLRIHCAEALGTPIVGD 368
           RVILAN++ +EASQEAITEYRVLGP INGCSWIELCPRTSRKHQLR+HCAEALGTPIVGD
Sbjct: 312 RVILANQSGLEASQEAITEYRVLGPMINGCSWIELCPRTSRKHQLRVHCAEALGTPIVGD 371

Query: 369 YKYGWFVHRRWKQMPRVDVEPISGRPYKLRRPEGLDVQKGSVLSKVPLLHLHCRELVLPN 428
           YKYGWFVHRRWKQMPRVD+EP +G+PYK+RRPEGLDVQKGSVLSKVPLLHLHCREL+LPN
Sbjct: 372 YKYGWFVHRRWKQMPRVDLEPTTGKPYKMRRPEGLDVQKGSVLSKVPLLHLHCRELILPN 431

Query: 429 IAKFLNVFYGKGPNR---PVSSFDSDLLRFVARMPSHMRISWNLMSSYLV 472
           IAKFL +   K  N+   P  S   D+LRFVA MP+HM+ISWNLMSSYLV
Sbjct: 432 IAKFLPILDPKPKNQNQYPKLSLKPDILRFVASMPNHMKISWNLMSSYLV 481

BLAST of Cucsa.088400 vs. TrEMBL
Match: A0A067JP47_JATCU (Uncharacterized protein OS=Jatropha curcas GN=JCGZ_21770 PE=4 SV=1)

HSP 1 Score: 716.5 bits (1848), Expect = 2.2e-203
Identity = 346/466 (74.25%), Postives = 396/466 (84.98%), Query Frame = 1

Query: 7   RHLLLATRCYSRISPPPPAYAKPIIRVSNNIAQLGSPKEGPKARQLLSLPPFPGYPLPGK 66
           + +L   R YSRI+ PPPAYA+P+IRVSNN+A LGSPK+GPK RQLLSLPPFPG PLPGK
Sbjct: 8   KRVLSKVRSYSRIAAPPPAYAQPVIRVSNNVAYLGSPKDGPKPRQLLSLPPFPGLPLPGK 67

Query: 67  NQVGVSGESTHVTAIRWVKYYFDEIQDSQIQSHFRKGLVQVECPQLGGLDEKGNLKPMRK 126
             V     ++ VTAI WVKYYFDEI DS IQSHF +GLVQ+EC Q GG         MRK
Sbjct: 68  TSV-----ASRVTAISWVKYYFDEIPDSVIQSHFNRGLVQMECMQHGGETF------MRK 127

Query: 127 IRSGEIMETGARIHLPVSVAEMKVARRFDTIPSGTLYPNADEIKYLQRLVKYKDSAIIVL 186
           I+  ++ME GAR+ +PVSVAE ++++RFDTIPSGTLYPNADEI+YLQRLVKYKDSAIIVL
Sbjct: 128 IKPSDVMEVGARVLIPVSVAETRISKRFDTIPSGTLYPNADEIEYLQRLVKYKDSAIIVL 187

Query: 187 NKPPKLPVKGTLPVHNSMDALADAALSYDYDDGPKLVHRLDRESSGILLMGRTKESVDHL 246
           NKPPKLP+KG LPVHNSMDALA AAL+YDYD+GPKLV RLDRESSG++LMGRTKESVDHL
Sbjct: 188 NKPPKLPIKGNLPVHNSMDALAAAALTYDYDEGPKLVQRLDRESSGLILMGRTKESVDHL 247

Query: 247 QWLFSNIRNAKFTCKAWEEACNATYQKYWALVIGCPREREGVISAPLSKVLLDDGKTERV 306
           QW+FS+ + +   CK W +AC AT Q+YWAL+IG P+E+EG+I APLSKVLL+DGKTER+
Sbjct: 248 QWMFSDQKKSNSYCKDWNDACQATCQRYWALIIGSPKEKEGLIRAPLSKVLLNDGKTERI 307

Query: 307 ILANKASIEASQEAITEYRVLGPTINGCSWIELCPRTSRKHQLRIHCAEALGTPIVGDYK 366
           +LA  +  EASQEA+TEYRVLGPTINGCSW+ELCP TSRKHQLR+HCAEALGTPIVGDYK
Sbjct: 308 VLAQNSGFEASQEALTEYRVLGPTINGCSWVELCPLTSRKHQLRVHCAEALGTPIVGDYK 367

Query: 367 YGWFVHRRWKQMPRVDVEPISGRPYKLRRPEGLDVQKGSVLSKVPLLHLHCRELVLPNIA 426
           YGWF+HRRWKQMPRVD+EP SG+PYKLRRPEG+DVQKGSVLSKVPLLHLHCRELVLPNIA
Sbjct: 368 YGWFMHRRWKQMPRVDIEPFSGKPYKLRRPEGIDVQKGSVLSKVPLLHLHCRELVLPNIA 427

Query: 427 KFLNVFYGKGPN-RPVSSFDSDLLRFVARMPSHMRISWNLMSSYLV 472
           KFL+V   K  N  P  S   DLLRFVA MP+HM+ISWNLMSSYLV
Sbjct: 428 KFLDVLNQKSKNIHPALSAKPDLLRFVASMPTHMKISWNLMSSYLV 462

BLAST of Cucsa.088400 vs. TrEMBL
Match: A0A061GAV8_THECC (Pseudouridine synthase family protein OS=Theobroma cacao GN=TCM_027759 PE=4 SV=1)

HSP 1 Score: 707.6 bits (1825), Expect = 1.0e-200
Identity = 345/475 (72.63%), Postives = 395/475 (83.16%), Query Frame = 1

Query: 1   MQRS-MRRHLLLATRCYSRISPPPPAYAKPIIRVSNNIAQLGSPKEGPKARQLLSLPPFP 60
           MQ+S +    L   R YSR++PPPPAYA P++RVSN++A LG+PK+GPK RQLLSLPPFP
Sbjct: 1   MQKSRLGWQFLSLVRHYSRVAPPPPAYADPVLRVSNSVAYLGAPKQGPKPRQLLSLPPFP 60

Query: 61  GYPLPGKNQVGVSGESTHVTAIRWVKYYFDEIQDSQIQSHFRKGLVQVECPQLGG--LDE 120
           GYPLPGKN     G +  VTAI W+KYYFDEI DS IQSHF KGLVQ+E    G   ++ 
Sbjct: 61  GYPLPGKNP----GGTARVTAISWLKYYFDEIPDSAIQSHFNKGLVQMESSNAGDTCIER 120

Query: 121 KGNLKPMRKIRSGEIMETGARIHLPVSVAEMKVARRFDTIPSGTLYPNADEIKYLQRLVK 180
            G +KP+RKI+  E+ME G R+ +PVS+AE ++++RFD IPSGTLYPN DEI YLQRLVK
Sbjct: 121 DGLMKPLRKIKHNEVMEVGTRVWVPVSIAETRISKRFDCIPSGTLYPNKDEIDYLQRLVK 180

Query: 181 YKDSAIIVLNKPPKLPVKGTLPVHNSMDALADAALSYDYDDGPKLVHRLDRESSGILLMG 240
           YKD AI+VLNKPPKLPVKG+LPVHNSMDALA AALSYD+D+GPKLVHRLDRESSG+LLMG
Sbjct: 181 YKDYAILVLNKPPKLPVKGSLPVHNSMDALAAAALSYDHDEGPKLVHRLDRESSGLLLMG 240

Query: 241 RTKESVDHLQWLFSNIRNAKFTCKAWEEACNATYQKYWALVIGCPREREGVISAPLSKVL 300
           RTKESV HL WLF+++   K +CKAW +AC+  YQ++WALVIG P+E+EG+I APLSK+L
Sbjct: 241 RTKESVAHLHWLFNDLNTVKSSCKAWNDACDVKYQRFWALVIGTPKEKEGLICAPLSKIL 300

Query: 301 LDDGKTERVILANKASIEASQEAITEYRVLGPTINGCSWIELCPRTSRKHQLRIHCAEAL 360
           LDDGKTERVILA    +EASQEAITEYRVLGP INGCSWIEL P TSRKHQLR+HCAEAL
Sbjct: 301 LDDGKTERVILAQNVGLEASQEAITEYRVLGPMINGCSWIELRPLTSRKHQLRVHCAEAL 360

Query: 361 GTPIVGDYKYGWFVHRRWKQMPRVDVEPISGRPYKLRRPEGLDVQKGSVLSKVPLLHLHC 420
           GTPIVGDYKYGWFVHRRWKQMPRVD+EP +G+PYKLRRPEGLDVQKGSVLSKVPLLHLHC
Sbjct: 361 GTPIVGDYKYGWFVHRRWKQMPRVDIEPTTGKPYKLRRPEGLDVQKGSVLSKVPLLHLHC 420

Query: 421 RELVLPNIAKFLNVFYGKGPNRPVS-SFDSDLLRFVARMPSHMRISWNLMSSYLV 472
           RELVLPNIAKFL V        P + S   DLLRFVA MP HM+ISWNLMSSYLV
Sbjct: 421 RELVLPNIAKFLPVLNKNTETLPPALSMKPDLLRFVATMPKHMKISWNLMSSYLV 471

BLAST of Cucsa.088400 vs. TAIR10
Match: AT1G78910.1 (AT1G78910.1 Pseudouridine synthase family protein)

HSP 1 Score: 655.2 bits (1689), Expect = 3.0e-188
Identity = 321/473 (67.86%), Postives = 389/473 (82.24%), Query Frame = 1

Query: 8   HLLLATRCYSRISPPPPAYAKPIIRVSNNIAQLGSPKEGPKARQLLSLPPFPGYPLPGKN 67
           +L +  R YSR++PPP +    +IRV+NN+A LG PK+GP  RQL+SLPPFPG+PLPGKN
Sbjct: 12  YLTVLIRRYSRVAPPPSS----VIRVTNNVAHLGPPKQGPLPRQLISLPPFPGHPLPGKN 71

Query: 68  QVGVSGEST----HVTAIRWVKYYFDEIQDSQIQSHFRKGLVQVECP--QLGGLDEKGNL 127
             G  G+      HVTAI WVKYYF+EI D  IQ+HF KGLVQ+E    +    +++   
Sbjct: 72  -AGADGDDGDSGGHVTAISWVKYYFEEIYDKAIQTHFTKGLVQMEFRGRRDASREKEDGA 131

Query: 128 KPMRKIRSGEIMETGARIHLPVSVAEMKVARRFDTIPSGTLYPNADEIKYLQRLVKYKDS 187
            PMRKI+  E+M+ G +I LPVS+AEM++++R+DTIPSGTLYPNADEI YLQRLV++KDS
Sbjct: 132 IPMRKIKHNEVMQIGDKIWLPVSIAEMRISKRYDTIPSGTLYPNADEIAYLQRLVRFKDS 191

Query: 188 AIIVLNKPPKLPVKGTLPVHNSMDALADAALSYDYDDGPKLVHRLDRESSGILLMGRTKE 247
           AIIVLNKPPKLPVKG +P+HNSMDALA AALS+  D+GP+LVHRLDRE+SG+L+MGRTKE
Sbjct: 192 AIIVLNKPPKLPVKGNVPIHNSMDALAAAALSFGNDEGPRLVHRLDRETSGLLVMGRTKE 251

Query: 248 SVDHLQWLFSNIRNAKFTCKAWEEACNATYQKYWALVIGCPREREGVISAPLSKVLLDDG 307
           S+D+L  +FS+ +    +CKAW +AC A YQ+YWALVIG P+E+EG+ISAPLSKVLLDDG
Sbjct: 252 SIDYLHSVFSDYKGRNSSCKAWNKACEAMYQQYWALVIGSPKEKEGLISAPLSKVLLDDG 311

Query: 308 KTERVILANKASIEASQEAITEYRVLGPTINGCSWIELCPRTSRKHQLRIHCAEALGTPI 367
           KT+RV+LA  +  EASQ+AITEY+VLGP INGCSW+EL P TSRKHQLR+HCAEALGTPI
Sbjct: 312 KTDRVVLAQGSGFEASQDAITEYKVLGPKINGCSWVELRPITSRKHQLRVHCAEALGTPI 371

Query: 368 VGDYKYGWFVHRRWKQMPRVDVEPISGRPYKLRRPEGLDVQKGSVLSKVPLLHLHCRELV 427
           VGDYKYGWFVH+RWKQMP+VD+EP +G+PYKLRRPEGLDVQKGSVLSKVPLLHLHCRE+V
Sbjct: 372 VGDYKYGWFVHKRWKQMPQVDIEPTTGKPYKLRRPEGLDVQKGSVLSKVPLLHLHCREMV 431

Query: 428 LPNIAKFLNVFYGKGPNRPVSS---FDSDLLRFVARMPSHMRISWNLMSSYLV 472
           LPNIAKFL+V   +    P+ +      DLLRFVA MPSHM+ISWNLMSSYLV
Sbjct: 432 LPNIAKFLHVM-NQQETEPLHTGIIDKPDLLRFVASMPSHMKISWNLMSSYLV 478

BLAST of Cucsa.088400 vs. TAIR10
Match: AT3G19440.1 (AT3G19440.1 Pseudouridine synthase family protein)

HSP 1 Score: 260.0 bits (663), Expect = 2.8e-69
Identity = 160/425 (37.65%), Postives = 241/425 (56.71%), Query Frame = 1

Query: 53  LSLPPFPGYP---------LPGKNQVGVSGESTHVTAIRWVKYYFDEIQDSQIQSHFRKG 112
           L+LPPF             L   + V  S +++  TA+RW+     ++  + +Q  FR  
Sbjct: 56  LTLPPFSPTIDGTAVGKDLLSDGDSVKSSTDNSKTTALRWILRCRPDLPRTLVQKLFRLR 115

Query: 113 LVQVECPQLGGLDEKGNLKPMRKIRSGEIMETGARIHLPVSVAEMKVARRFDTIPSGTLY 172
            V+ E       DE    + ++++ + E +  G RI+LP+SV       +       +  
Sbjct: 116 QVRREMSMSVDGDELQRSQ-LKRVAAKESLNVGDRIYLPLSVDND--TPQTPPAKKESFQ 175

Query: 173 PNADEIKYLQRLVKYKDSAIIVLNKPPKLPVKGTLPVHNSMDALADAALSYDYDDGPKLV 232
            + +E K++  LV YKD AIIVLNKP  L V+G   +  S+D LA + L +D  + P+LV
Sbjct: 176 CSDEERKFVCSLVLYKDPAIIVLNKPHGLAVQGGSGIKTSIDELAASCLKFDKSESPRLV 235

Query: 233 HRLDRESSGILLMGRTKESVDHLQWLFSNIRNAKFTCKAWEEACNATYQKYWALVIGCPR 292
           HRLDR+ SG+L++ RT+ +   L  +F   +    +    ++   +  +KY ALVIGCP 
Sbjct: 236 HRLDRDCSGLLVLARTQTAATVLHSIFRE-KTTGASAYGVKKNVKSLKRKYMALVIGCPP 295

Query: 293 EREGVISAPLSKVLLDDGKTERVILANKASIEASQEAITEYRVLGPTINGCSWIELCPRT 352
            + G ISAPL KV++DDGK+ER+ + +   + ++Q AITEYRV+  + +G +W+EL P T
Sbjct: 296 RQRGQISAPLRKVVVDDGKSERITVNDNGELVSTQHAITEYRVIESSPHGYTWLELRPLT 355

Query: 353 SRKHQLRIHCAEALGTPIVGDYKYGWFVHRRWKQMPRVDVEPISGRPYKLRRPEGLDVQK 412
            RKHQLR+HCAE LGTPIVGDYKYGW  H+  +     +  P      +   P GLD+  
Sbjct: 356 GRKHQLRVHCAEVLGTPIVGDYKYGWQAHKAREPFVSSENNPTK----QSSSPFGLDLDG 415

Query: 413 GSVLSKVPLLHLHCRELVLPNIAKFLNVFYGKGPNRPVSSFDSDLLRFVARMPSHMRISW 469
           G V SK P LHLH +++ LPNI++ L        +  +S  DS  L+F A +PSHM++S+
Sbjct: 416 GDVSSKQPHLHLHSKQIDLPNISQLLEKMQ-VSSDSDISDLDS--LKFDAPLPSHMQLSF 469

BLAST of Cucsa.088400 vs. TAIR10
Match: AT1G76050.2 (AT1G76050.2 Pseudouridine synthase family protein)

HSP 1 Score: 52.4 bits (124), Expect = 9.0e-07
Identity = 58/243 (23.87%), Postives = 98/243 (40.33%), Query Frame = 1

Query: 162 LYPNADEIKYLQRLVKYKDSAIIVLNKPPKLPVK-------GTLP---VHNSMDALADAA 221
           L P   E + +   + Y+D  ++V+NKP  + V        GTL    +H+      D +
Sbjct: 136 LQPLKAEAEDIPLDIVYEDKHVLVVNKPAHMVVHPAPGNPTGTLVNGILHHCSLPCVDYS 195

Query: 222 LSYDYDDG-----------------------PKLVHRLDRESSGILLMGRTKESVDHLQW 281
            S + DD                        P +VHRLD+ ++G+L++ + + S  HL  
Sbjct: 196 NSEEDDDSDEETFSDDEEMTTSPSSYAASVRPGIVHRLDKGTTGLLVVAKDEHSHAHLAE 255

Query: 282 LFSNIRNAKFTCKAWEEACNATYQKYWALVIGCPREREGVISAPLSKVLLDDGKTERVIL 341
            F                 +   + Y +L  G P   +G I  P+ +    D      + 
Sbjct: 256 QFK---------------LHTIERVYVSLTTGVPSPPQGRIEIPIGR----DSSNRIRMA 315

Query: 342 ANKASIEA--SQEAITEYRVLGPTINGCS-WIELCPRTSRKHQLRIHCAEALGTPIVGDY 369
           A    +    ++ A + Y+V+     G S  +E    T R HQ+R H A+ +G P++GD 
Sbjct: 316 AIPGGVRGGRARHAASRYKVIETFAGGGSALVEWRLETGRTHQIRAH-AKYMGVPLLGDE 358

BLAST of Cucsa.088400 vs. NCBI nr
Match: gi|778672934|ref|XP_011649894.1| (PREDICTED: RNA pseudouridine synthase 3, mitochondrial isoform X1 [Cucumis sativus])

HSP 1 Score: 962.6 bits (2487), Expect = 2.5e-277
Identity = 470/471 (99.79%), Postives = 471/471 (100.00%), Query Frame = 1

Query: 1   MQRSMRRHLLLATRCYSRISPPPPAYAKPIIRVSNNIAQLGSPKEGPKARQLLSLPPFPG 60
           MQRSMRRHLLLATRCYSRISPPPPAYAKPIIRVSNNIAQLGSPKEGPKARQLLSLPPFPG
Sbjct: 1   MQRSMRRHLLLATRCYSRISPPPPAYAKPIIRVSNNIAQLGSPKEGPKARQLLSLPPFPG 60

Query: 61  YPLPGKNQVGVSGESTHVTAIRWVKYYFDEIQDSQIQSHFRKGLVQVECPQLGGLDEKGN 120
           YPLPGKNQVGVSGESTHVTAIRWVKYYFDEIQDSQIQSHFRKGLVQVECPQLGGLDEKGN
Sbjct: 61  YPLPGKNQVGVSGESTHVTAIRWVKYYFDEIQDSQIQSHFRKGLVQVECPQLGGLDEKGN 120

Query: 121 LKPMRKIRSGEIMETGARIHLPVSVAEMKVARRFDTIPSGTLYPNADEIKYLQRLVKYKD 180
           LKPMRKIRSGEIMETGARIHLPVSVAEMKVARRFDTIPSGTLYPNADEIKYLQRLVKYKD
Sbjct: 121 LKPMRKIRSGEIMETGARIHLPVSVAEMKVARRFDTIPSGTLYPNADEIKYLQRLVKYKD 180

Query: 181 SAIIVLNKPPKLPVKGTLPVHNSMDALADAALSYDYDDGPKLVHRLDRESSGILLMGRTK 240
           SAIIVLNKPPKLPVKGTLPVHNSMDALADAALSYDYDDGPKLVHRLDRESSGILLMGRTK
Sbjct: 181 SAIIVLNKPPKLPVKGTLPVHNSMDALADAALSYDYDDGPKLVHRLDRESSGILLMGRTK 240

Query: 241 ESVDHLQWLFSNIRNAKFTCKAWEEACNATYQKYWALVIGCPREREGVISAPLSKVLLDD 300
           ESVDHLQWLFSNIRNAKFTCKAWEEACNATYQKYWALVIGCPREREGVISAPLSKVLLDD
Sbjct: 241 ESVDHLQWLFSNIRNAKFTCKAWEEACNATYQKYWALVIGCPREREGVISAPLSKVLLDD 300

Query: 301 GKTERVILANKASIEASQEAITEYRVLGPTINGCSWIELCPRTSRKHQLRIHCAEALGTP 360
           GKTERV+LANKASIEASQEAITEYRVLGPTINGCSWIELCPRTSRKHQLRIHCAEALGTP
Sbjct: 301 GKTERVVLANKASIEASQEAITEYRVLGPTINGCSWIELCPRTSRKHQLRIHCAEALGTP 360

Query: 361 IVGDYKYGWFVHRRWKQMPRVDVEPISGRPYKLRRPEGLDVQKGSVLSKVPLLHLHCREL 420
           IVGDYKYGWFVHRRWKQMPRVDVEPISGRPYKLRRPEGLDVQKGSVLSKVPLLHLHCREL
Sbjct: 361 IVGDYKYGWFVHRRWKQMPRVDVEPISGRPYKLRRPEGLDVQKGSVLSKVPLLHLHCREL 420

Query: 421 VLPNIAKFLNVFYGKGPNRPVSSFDSDLLRFVARMPSHMRISWNLMSSYLV 472
           VLPNIAKFLNVFYGKGPNRPVSSFDSDLLRFVARMPSHMRISWNLMSSYLV
Sbjct: 421 VLPNIAKFLNVFYGKGPNRPVSSFDSDLLRFVARMPSHMRISWNLMSSYLV 471

BLAST of Cucsa.088400 vs. NCBI nr
Match: gi|700207975|gb|KGN63094.1| (hypothetical protein Csa_2G402020 [Cucumis sativus])

HSP 1 Score: 962.6 bits (2487), Expect = 2.5e-277
Identity = 470/471 (99.79%), Postives = 471/471 (100.00%), Query Frame = 1

Query: 1   MQRSMRRHLLLATRCYSRISPPPPAYAKPIIRVSNNIAQLGSPKEGPKARQLLSLPPFPG 60
           MQRSMRRHLLLATRCYSRISPPPPAYAKPIIRVSNNIAQLGSPKEGPKARQLLSLPPFPG
Sbjct: 73  MQRSMRRHLLLATRCYSRISPPPPAYAKPIIRVSNNIAQLGSPKEGPKARQLLSLPPFPG 132

Query: 61  YPLPGKNQVGVSGESTHVTAIRWVKYYFDEIQDSQIQSHFRKGLVQVECPQLGGLDEKGN 120
           YPLPGKNQVGVSGESTHVTAIRWVKYYFDEIQDSQIQSHFRKGLVQVECPQLGGLDEKGN
Sbjct: 133 YPLPGKNQVGVSGESTHVTAIRWVKYYFDEIQDSQIQSHFRKGLVQVECPQLGGLDEKGN 192

Query: 121 LKPMRKIRSGEIMETGARIHLPVSVAEMKVARRFDTIPSGTLYPNADEIKYLQRLVKYKD 180
           LKPMRKIRSGEIMETGARIHLPVSVAEMKVARRFDTIPSGTLYPNADEIKYLQRLVKYKD
Sbjct: 193 LKPMRKIRSGEIMETGARIHLPVSVAEMKVARRFDTIPSGTLYPNADEIKYLQRLVKYKD 252

Query: 181 SAIIVLNKPPKLPVKGTLPVHNSMDALADAALSYDYDDGPKLVHRLDRESSGILLMGRTK 240
           SAIIVLNKPPKLPVKGTLPVHNSMDALADAALSYDYDDGPKLVHRLDRESSGILLMGRTK
Sbjct: 253 SAIIVLNKPPKLPVKGTLPVHNSMDALADAALSYDYDDGPKLVHRLDRESSGILLMGRTK 312

Query: 241 ESVDHLQWLFSNIRNAKFTCKAWEEACNATYQKYWALVIGCPREREGVISAPLSKVLLDD 300
           ESVDHLQWLFSNIRNAKFTCKAWEEACNATYQKYWALVIGCPREREGVISAPLSKVLLDD
Sbjct: 313 ESVDHLQWLFSNIRNAKFTCKAWEEACNATYQKYWALVIGCPREREGVISAPLSKVLLDD 372

Query: 301 GKTERVILANKASIEASQEAITEYRVLGPTINGCSWIELCPRTSRKHQLRIHCAEALGTP 360
           GKTERV+LANKASIEASQEAITEYRVLGPTINGCSWIELCPRTSRKHQLRIHCAEALGTP
Sbjct: 373 GKTERVVLANKASIEASQEAITEYRVLGPTINGCSWIELCPRTSRKHQLRIHCAEALGTP 432

Query: 361 IVGDYKYGWFVHRRWKQMPRVDVEPISGRPYKLRRPEGLDVQKGSVLSKVPLLHLHCREL 420
           IVGDYKYGWFVHRRWKQMPRVDVEPISGRPYKLRRPEGLDVQKGSVLSKVPLLHLHCREL
Sbjct: 433 IVGDYKYGWFVHRRWKQMPRVDVEPISGRPYKLRRPEGLDVQKGSVLSKVPLLHLHCREL 492

Query: 421 VLPNIAKFLNVFYGKGPNRPVSSFDSDLLRFVARMPSHMRISWNLMSSYLV 472
           VLPNIAKFLNVFYGKGPNRPVSSFDSDLLRFVARMPSHMRISWNLMSSYLV
Sbjct: 493 VLPNIAKFLNVFYGKGPNRPVSSFDSDLLRFVARMPSHMRISWNLMSSYLV 543

BLAST of Cucsa.088400 vs. NCBI nr
Match: gi|659081205|ref|XP_008441208.1| (PREDICTED: RNA pseudouridine synthase 3, mitochondrial [Cucumis melo])

HSP 1 Score: 917.1 bits (2369), Expect = 1.2e-263
Identity = 453/473 (95.77%), Postives = 460/473 (97.25%), Query Frame = 1

Query: 1   MQRSMRRHLLLATRCYSRISPPPPAYAKPIIRVSNNIAQLGSPKEGPKARQLLSLPPFPG 60
           MQRS+RRHLLLATR YSRISPPPPAYAKPIIRVSNNIAQLGSPKEGPKARQLLSLPPFPG
Sbjct: 1   MQRSIRRHLLLATRYYSRISPPPPAYAKPIIRVSNNIAQLGSPKEGPKARQLLSLPPFPG 60

Query: 61  YPLPGKNQVGVSGESTHVTAIRWVKYYFDEIQDSQIQSHFRKGLVQVECPQLGGLDEKGN 120
           +PLPGKNQVGVSGESTHVTAIRWVKYYFDEIQDSQIQS FRKGLVQVECPQLGG DE GN
Sbjct: 61  HPLPGKNQVGVSGESTHVTAIRWVKYYFDEIQDSQIQSLFRKGLVQVECPQLGGFDENGN 120

Query: 121 LKPMRKIRSGEIMETGARIHLPVSVAEMKVARRFDTIPSGTLYPNADEIKYLQRLVKYKD 180
           LKPMRKIRSGEIME GARIHLPVSVAEMKV+RRFDTIPSGTLYPNADEIKYLQRLVKYKD
Sbjct: 121 LKPMRKIRSGEIMEIGARIHLPVSVAEMKVSRRFDTIPSGTLYPNADEIKYLQRLVKYKD 180

Query: 181 SAIIVLNKPPKLPVKGTLPVHNSMDALADAALSYDYDDGPKLVHRLDRESSGILLMGRTK 240
           SAIIVLNKPPKLPVKGTLPVHNSMDALA AALSYDYD GPKLVHRLDRESSGILLMGRTK
Sbjct: 181 SAIIVLNKPPKLPVKGTLPVHNSMDALAAAALSYDYDYGPKLVHRLDRESSGILLMGRTK 240

Query: 241 ESVDHLQWLFSNIRNAKFTCKAWEEACNATYQKYWALVIGCPREREGVISAPLSKVLLDD 300
           ESVDHLQWLFSNIRN KFTCKAWEEACNATYQKYWALVIG PREREG+ISAPLSKVLLDD
Sbjct: 241 ESVDHLQWLFSNIRNGKFTCKAWEEACNATYQKYWALVIGRPREREGLISAPLSKVLLDD 300

Query: 301 GKTERVILANKASIEASQEAITEYRVLGPTINGCSWIELCPRTSRKHQLRIHCAEALGTP 360
           GKTERV+LANKAS+EASQEAITEYRVLGPTINGCSWIELCPRTSRKHQLRIHCAEALGTP
Sbjct: 301 GKTERVVLANKASLEASQEAITEYRVLGPTINGCSWIELCPRTSRKHQLRIHCAEALGTP 360

Query: 361 IVGDYKYGWFVHRRWKQMPRVDVEPISGRPYKLRRPEGLDVQKGSVLSKVPLLHLHCREL 420
           IVGDYKYGWFVHRRWKQMPRVDVEPISGRPYKLRRPEGLDVQKGSVLSKVPLLHLHCREL
Sbjct: 361 IVGDYKYGWFVHRRWKQMPRVDVEPISGRPYKLRRPEGLDVQKGSVLSKVPLLHLHCREL 420

Query: 421 VLPNIAKFLNVFYGKGP--NRPVSSFDSDLLRFVARMPSHMRISWNLMSSYLV 472
           VLPNIAKFLNVFY KGP  +RPVSSFD DLLRFVARMPSHMRISWNLMSSYLV
Sbjct: 421 VLPNIAKFLNVFYDKGPISHRPVSSFDFDLLRFVARMPSHMRISWNLMSSYLV 473

BLAST of Cucsa.088400 vs. NCBI nr
Match: gi|778672937|ref|XP_011649895.1| (PREDICTED: RNA pseudouridine synthase 3, mitochondrial isoform X2 [Cucumis sativus])

HSP 1 Score: 892.9 bits (2306), Expect = 2.4e-256
Identity = 443/471 (94.06%), Postives = 444/471 (94.27%), Query Frame = 1

Query: 1   MQRSMRRHLLLATRCYSRISPPPPAYAKPIIRVSNNIAQLGSPKEGPKARQLLSLPPFPG 60
           MQRSMRRHLLLATRCYSRISPPPPAYAKPIIRVSNNIAQLGSPKEGPKARQLLSLPPFPG
Sbjct: 1   MQRSMRRHLLLATRCYSRISPPPPAYAKPIIRVSNNIAQLGSPKEGPKARQLLSLPPFPG 60

Query: 61  YPLPGKNQVGVSGESTHVTAIRWVKYYFDEIQDSQIQSHFRKGLVQVECPQLGGLDEKGN 120
           YPLPGKNQVGVSGESTHVTAIRWVKYYFDEIQDSQIQSHFRKGLVQVECPQLGGLDEKGN
Sbjct: 61  YPLPGKNQVGVSGESTHVTAIRWVKYYFDEIQDSQIQSHFRKGLVQVECPQLGGLDEKGN 120

Query: 121 LKPMRKIRSGEIMETGARIHLPVSVAEMKVARRFDTIPSGTLYPNADEIKYLQRLVKYKD 180
           LKPMRKIRSGEIMETGARIHLPVSVAEMKVARRFDTIPSGTLYPNADEIKYLQRLVKYKD
Sbjct: 121 LKPMRKIRSGEIMETGARIHLPVSVAEMKVARRFDTIPSGTLYPNADEIKYLQRLVKYKD 180

Query: 181 SAIIVLNKPPKLPVKGTLPVHNSMDALADAALSYDYDDGPKLVHRLDRESSGILLMGRTK 240
           SAIIVLNKPPKLPVK                           VHRLDRESSGILLMGRTK
Sbjct: 181 SAIIVLNKPPKLPVK---------------------------VHRLDRESSGILLMGRTK 240

Query: 241 ESVDHLQWLFSNIRNAKFTCKAWEEACNATYQKYWALVIGCPREREGVISAPLSKVLLDD 300
           ESVDHLQWLFSNIRNAKFTCKAWEEACNATYQKYWALVIGCPREREGVISAPLSKVLLDD
Sbjct: 241 ESVDHLQWLFSNIRNAKFTCKAWEEACNATYQKYWALVIGCPREREGVISAPLSKVLLDD 300

Query: 301 GKTERVILANKASIEASQEAITEYRVLGPTINGCSWIELCPRTSRKHQLRIHCAEALGTP 360
           GKTERV+LANKASIEASQEAITEYRVLGPTINGCSWIELCPRTSRKHQLRIHCAEALGTP
Sbjct: 301 GKTERVVLANKASIEASQEAITEYRVLGPTINGCSWIELCPRTSRKHQLRIHCAEALGTP 360

Query: 361 IVGDYKYGWFVHRRWKQMPRVDVEPISGRPYKLRRPEGLDVQKGSVLSKVPLLHLHCREL 420
           IVGDYKYGWFVHRRWKQMPRVDVEPISGRPYKLRRPEGLDVQKGSVLSKVPLLHLHCREL
Sbjct: 361 IVGDYKYGWFVHRRWKQMPRVDVEPISGRPYKLRRPEGLDVQKGSVLSKVPLLHLHCREL 420

Query: 421 VLPNIAKFLNVFYGKGPNRPVSSFDSDLLRFVARMPSHMRISWNLMSSYLV 472
           VLPNIAKFLNVFYGKGPNRPVSSFDSDLLRFVARMPSHMRISWNLMSSYLV
Sbjct: 421 VLPNIAKFLNVFYGKGPNRPVSSFDSDLLRFVARMPSHMRISWNLMSSYLV 444

BLAST of Cucsa.088400 vs. NCBI nr
Match: gi|1009156134|ref|XP_015896084.1| (PREDICTED: RNA pseudouridine synthase 3, mitochondrial [Ziziphus jujuba])

HSP 1 Score: 726.1 bits (1873), Expect = 4.0e-206
Identity = 347/469 (73.99%), Postives = 400/469 (85.29%), Query Frame = 1

Query: 6   RRHLLLATRCYSRISPPPPAYAKPIIRVSNNIAQLGSPKEGPKARQLLSLPPFPGYPLPG 65
           +R + L+ R YSRISPPPP   KP+IRVSNN+A+LG PKEGPK RQLLSLPPFPG+PLPG
Sbjct: 7   KRPIWLSIRKYSRISPPPPVNVKPVIRVSNNVARLGCPKEGPKPRQLLSLPPFPGFPLPG 66

Query: 66  KNQVGVSGESTHVTAIRWVKYYFDEIQDSQIQSHFRKGLVQVECPQLGG--LDEKGNLKP 125
           KN V V G S HVTAI WVKYYF  ++D  IQSHF KGLV  EC  +G   ++++G +KP
Sbjct: 67  KNMVTVPGRSPHVTAISWVKYYFYGVKDDVIQSHFNKGLVHRECSNMGESIVEKEGQMKP 126

Query: 126 MRKIRSGEIMETGARIHLPVSVAEMKVARRFDTIPSGTLYPNADEIKYLQRLVKYKDSAI 185
           MR+++  E+M+ G R+H+PVSVAE +++RRFDTIPSGTLYPNADEIKYLQRLVKY+D AI
Sbjct: 127 MREVKPSEVMKVGDRVHVPVSVAESRISRRFDTIPSGTLYPNADEIKYLQRLVKYRDWAI 186

Query: 186 IVLNKPPKLPVKGTLPVHNSMDALADAALSYDYDDGPKLVHRLDRESSGILLMGRTKESV 245
           IVLNKPP+LPVKG LP+HNSMDALA AALSY Y++GPKLVHRLDRESSG+++MGRTKES+
Sbjct: 187 IVLNKPPRLPVKGHLPIHNSMDALAAAALSYGYNEGPKLVHRLDRESSGLIIMGRTKESI 246

Query: 246 DHLQWLFSNIRNAKFTCKAWEEACNATYQKYWALVIGCPREREGVISAPLSKVLLDDGKT 305
           DHL WLFSN +      KAW +AC ATYQKYWALVIG P+E+EG+I APLSKVLLDDGKT
Sbjct: 247 DHLNWLFSNYKEVNTPHKAWNDACEATYQKYWALVIGSPKEKEGLICAPLSKVLLDDGKT 306

Query: 306 ERVILANKASIEASQEAITEYRVLGPTINGCSWIELCPRTSRKHQLRIHCAEALGTPIVG 365
           ERVILA+++  E SQ+AITEYRVLGP INGCSW+EL P TSRKHQLR+HCAEALGTPIVG
Sbjct: 307 ERVILAHQSGFEPSQKAITEYRVLGPMINGCSWVELHPHTSRKHQLRVHCAEALGTPIVG 366

Query: 366 DYKYGWFVHRRWKQMPRVDVEPISGRPYKLRRPEGLDVQKGSVLSKVPLLHLHCRELVLP 425
           DYKYGWFVHRRWKQMPRVD+E  +G+PYKLRRP+GLDVQKGSVLSKVPLLHLHCRELVLP
Sbjct: 367 DYKYGWFVHRRWKQMPRVDIEATTGKPYKLRRPDGLDVQKGSVLSKVPLLHLHCRELVLP 426

Query: 426 NIAKFLNVFYGKGPNRPVS-SFDSDLLRFVARMPSHMRISWNLMSSYLV 472
           NIAKFL VF GK   +    S + D+LRFVA MPSHM+ISWNLMSSYLV
Sbjct: 427 NIAKFLKVFNGKSEYQSTEVSLERDVLRFVASMPSHMKISWNLMSSYLV 475